Datasets:

EMBO
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SourceData

<article doi="10.1038/emboj.2009.113" abstract="Correction to:The EMBO Journal (2009) 28, 677–685. doi:10.1038/emboj.2009.8"><fig id="1840"><title></title><label>Figure 1</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1840"/><sd-panel panel_id="4710">(A) <sd-tag id="sdTag1" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag>-<sd-tag id="sdTag2" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag3" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> cells were left untreated (Control) or treated with 5 μM <sd-tag id="sdTag4" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="rapamycin" ext_ids="CHEBI:9168" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="sirolimus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">rapamycin</sd-tag> (Rapa), 500 ng/ml <sd-tag id="sdTag5" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> or 10 ng/ml <sd-tag id="sdTag6" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TNF" ext_ids="P01375" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNF" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TNF</sd-tag> for 24 h. When indicated, 10 mM <sd-tag id="sdTag8" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="3‐MA" ext_ids="CHEBI:38635" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="3-methyladenine" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">3‐MA</sd-tag> or 5 μg/ml <sd-tag id="sdTag9">TRAIL‐R2 antagonist</sd-tag> antibody was added 1 h before the drugs. Representative confocal images (20 μm scale bars) and the percentages of cells with <sd-tag id="sdTag11" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9BXW4///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3C///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> translocation are shown.<graphic href="https://api.sourcedata.io/file.php?panel_id=4710"/></sd-panel><sd-panel panel_id="4711">(B) <sd-tag id="sdTag13" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag>-<sd-tag id="sdTag14" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag15" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9BXW4///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3C///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> cells were transfected with the indicated siRNAs for 48 h and analysed by <sd-tag id="sdTag19" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag> (left), or stimulated with 500 ng/ml <sd-tag id="sdTag16" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for 24 h and analysed for <sd-tag id="sdTag18" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="81631///84557///440738" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3B///MAP1LC3A///MAP1LC3C" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> translocation (right).<graphic href="https://api.sourcedata.io/file.php?panel_id=4711"/></sd-panel><sd-panel panel_id="4712">(C) <sd-tag id="sdTag28" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag> cells left untreated (Control) or treated with 500 ng/ml <sd-tag id="sdTag29" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> or 5 μM <sd-tag id="sdTag30" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="rapamycin" ext_ids="CHEBI:9168" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="sirolimus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">rapamycin</sd-tag> (Rapa) for 24 h were analysed for <sd-tag id="sdTag32" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9BXW4///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3C///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>‐I, <sd-tag id="sdTag33" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9BXW4///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3C///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>‐II and <sd-tag id="sdTag35" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="tubulin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">tubulin</sd-tag> by <sd-tag id="sdTag34" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>. <sd-tag id="sdTag36" source="sdapi" category="entity" entity_type="molecule" role="component" text="Pepstatin A" ext_ids="CHEBI:7989" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="Pepstatin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Pepstatin A</sd-tag> and <sd-tag id="sdTag37" source="sdapi" category="entity" entity_type="molecule" role="component" text="E64d" ext_ids="CHEBI:30270" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="E64" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">E64d</sd-tag> were added to the cells 4 h before harvesting. Similar results were obtained in two independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=4712"/></sd-panel><sd-panel panel_id="4713">(D) <sd-tag id="sdTag39" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag> cells were treated with 5 μM <sd-tag id="sdTag40" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="rapamycin" ext_ids="CHEBI:9168" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="sirolimus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">rapamycin</sd-tag> (Rapa) or indicated concentrations of <sd-tag id="sdTag41" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for 24 h, and the increase in the degradation of long‐lived proteins as compared with the untreated cultures (Control) was measured.<graphic href="https://api.sourcedata.io/file.php?panel_id=4713"/></sd-panel><sd-panel panel_id="4714">(E) <sd-tag id="sdTag45" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag>-<sd-tag id="sdTag46" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag47" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> cells transfected with the indicated siRNAs were left untreated 48 h later (Control) or treated with 500 ng/ml <sd-tag id="sdTag49" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for 24 h and analysed for the DNA content by <sd-tag id="sdTag54" source="sdapi" category="assay" entity_type="" role="" text="flow cytometry" ext_ids="BAO_0000005" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="flow cytometry" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">flow cytometry</sd-tag>. The percentage of apoptotic cells with sub‐G1 DNA content is shown. The values represent mean±s.d. for three (A, D) or four (B, E) independent experiments. *P‐value 0.05, **P‐value 0.01 and ***P‐value 0.001 as compared with control sample (A-left panel and D), with <sd-tag id="sdTag51" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag>‐treated cells without anti‐<sd-tag id="sdTag55" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag>‐R2 (A-right panel) or with <sd-tag id="sdTag56" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag>‐treated cells without siRNA (B, E).<graphic href="https://api.sourcedata.io/file.php?panel_id=4714"/></sd-panel></fig><fig id="1841"><title></title><label>Figure 2</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1841"/><sd-panel panel_id="4715">(A) Protein lysates from <sd-tag id="sdTag63" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag> cells left untreated or treated with 500 ng/ml <sd-tag id="sdTag64" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> or 5 μM <sd-tag id="sdTag65" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="rapamycin" ext_ids="CHEBI:9168" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="sirolimus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">rapamycin</sd-tag> (Rapa, positive control) for 24 h were analysed by <sd-tag id="sdTag67" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag> for phosphorylated p70S6K (P‐p70S6K), total <sd-tag id="sdTag61" source="sdapi" category="entity" entity_type="protein" role="assayed" text="70S6K" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">p70S6K</sd-tag> and <sd-tag id="sdTag62" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="GAPDH" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GAPDH</sd-tag> (loading control).<graphic href="https://api.sourcedata.io/file.php?panel_id=4715"/></sd-panel><sd-panel panel_id="28538">(B) <sd-tag id="sdTag307" source="sdapi" category="assay" entity_type="" role="" text="Immunoblot" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">Immunoblot</sd-tag> analysis of the levels of P‐<sd-tag id="sdTag308" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="O00763///Q13085" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACB///ACACA" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag> and <sd-tag id="sdTag309" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="tubulin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">tubulin</sd-tag> (loading control) in lysates from <sd-tag id="sdTag310" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag> cells left untreated or treated with 500 ng/ml <sd-tag id="sdTag311" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for 24 h, 1 mM <sd-tag id="sdTag313" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="AICAR" ext_ids="CHEBI:18406" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="AICA ribonucleotide" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">AICAR</sd-tag> for 4 h (positive control) or 5 μM <sd-tag id="sdTag312" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="rapamycin" ext_ids="CHEBI:9168" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="sirolimus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">rapamycin</sd-tag> for 24 h (negative control).<graphic href="https://api.sourcedata.io/file.php?panel_id=28538"/></sd-panel><sd-panel panel_id="4717">(C) <sd-tag id="sdTag78" source="sdapi" category="assay" entity_type="" role="" text="Immunoblot" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">Immunoblot</sd-tag> analysis of the level of P‐<sd-tag id="sdTag295" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="Q13085///O00763" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACA///ACACB" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag> and <sd-tag id="sdTag80" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="GAPDH" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GAPDH</sd-tag> (loading control) in lysates from <sd-tag id="sdTag81" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag> cells treated with 500 ng/ml <sd-tag id="sdTag82" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for the indicated times.<graphic href="https://api.sourcedata.io/file.php?panel_id=4717"/></sd-panel><sd-panel panel_id="4718">(D) <sd-tag id="sdTag83" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag> cells were transfected with indicated siRNAs for 48 h and analysed by <sd-tag id="sdTag85" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag> for the indicated proteins immediately (upper panel) or after additional 24 h (middle panel) or 2 h (lower panel) incubation with (+) or without (−) 500 ng/ml <sd-tag id="sdTag87" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=4718"/></sd-panel><sd-panel panel_id="4719">(E) <sd-tag id="sdTag95" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag>-<sd-tag id="sdTag96" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag97" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9BXW4///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3C///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> cells transfected with indicated siRNAs for 48 h and incubated for an additional 24 h with or without 500 ng/ml <sd-tag id="sdTag100" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> were analysed for <sd-tag id="sdTag101" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> translocation (top) and the DNA content (bottom). The values represent mean±s.d. for four independent experiments. *P‐value 0.05 and ***P‐value 0.001 as compared with <sd-tag id="sdTag102" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag>‐treated cells without siRNA. Similar results were obtained in two (A, B and D) or three (C, D) independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=4719"/></sd-panel></fig><fig id="1842"><title></title><label>Figure 3</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1842"/><sd-panel panel_id="4720">(A) <sd-tag id="sdTag107" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag>-<sd-tag id="sdTag108" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag109" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> cells were transfected with indicated siRNAs for 48 h and analysed for the indicated mRNAs (top) and proteins (bottom by <sd-tag id="sdTag106" source="sdapi" category="assay" entity_type="" role="" text="RT–PCR" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RT-PCR</sd-tag> and <sd-tag id="sdTag111" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>, respectively. Similar results were obtained in three independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=4720"/></sd-panel><sd-panel panel_id="4721">(B) <sd-tag id="sdTag316" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag>-<sd-tag id="sdTag122" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag123" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> cells transfected with indicated siRNAs for 48 h, and left untreated or treated with 500 ng/ml <sd-tag id="sdTag125" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for 2 h, 10 μM <sd-tag id="sdTag118" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="ionomycin" ext_ids="CHEBI:63954" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ionomycin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ionomycin</sd-tag> for 24 h or starved for <sd-tag id="sdTag119" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="amino acids" ext_ids="CHEBI:33709" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="amino acid" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">amino acids</sd-tag> and <sd-tag id="sdTag120" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> for 24 h (starvation) were analysed for P‐<sd-tag id="sdTag296" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="Q13085///O00763" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACA///ACACB" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag> and <sd-tag id="sdTag129" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="tubulin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">tubulin</sd-tag> (loading control) expression by <sd-tag id="sdTag130" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>. Similar results were obtained in two independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=4721"/></sd-panel><sd-panel panel_id="4722">(C) Cells transfected as in (B) and left untreated or treated with 500 ng/ml <sd-tag id="sdTag134" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag>, 10 μM <sd-tag id="sdTag135" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="ionomycin" ext_ids="CHEBI:63954" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ionomycin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ionomycin</sd-tag> or starved for <sd-tag id="sdTag136" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="amino acids" ext_ids="CHEBI:33709" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="amino acid" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">amino acids</sd-tag> and <sd-tag id="sdTag137" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> for 24 h were analysed for <sd-tag id="sdTag139" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9BXW4///Q9GZQ8///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> translocation. The values represent mean±s.d. of three independent experiments. ***P‐value 0.001 as compared with cells treated in a same way, but transfected without siRNA.<graphic href="https://api.sourcedata.io/file.php?panel_id=4722"/></sd-panel></fig><fig id="1843"><title></title><label>Figure 4</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1843"/><sd-panel panel_id="4723">(A) The lysates of <sd-tag id="sdTag151" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag> cells treated with 500 ng/ml <sd-tag id="sdTag152" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for the indicated times were analysed by <sd-tag id="sdTag153" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>. The control lane (0 min <sd-tag id="sdTag154" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag>) originates from the same blot and has been treated and exposed identically. Similar results were obtained in three independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=4723"/></sd-panel><sd-panel panel_id="4724">(B, C) The lysates of <sd-tag id="sdTag161" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag>-<sd-tag id="sdTag162" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag163" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> cells transfected with indicated siRNAs for 48 h and left untreated (−) or treated with 500 ng/ml <sd-tag id="sdTag165" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for 2 h were analysed by <sd-tag id="sdTag167" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>. (C, lower panel) <sd-tag id="sdTag168" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag>-<sd-tag id="sdTag169" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag170" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> cells were transfected with indicated siRNAs for 48 h and analysed for the indicated mRNAs by <sd-tag id="sdTag172" source="sdapi" category="assay" entity_type="" role="" text="RT–PCR" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RT-PCR</sd-tag>. Similar results were obtained in two independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=4724"/></sd-panel><sd-panel panel_id="4725">(D, E) <sd-tag id="sdTag318" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag>-<sd-tag id="sdTag185" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag186" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> cells were transfected with indicated siRNAs for 48 h, treated as in Figure 3C and analysed for <sd-tag id="sdTag188" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9BXW4///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3C///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> translocation (left) and sub‐G1 DNA content (right). The values represent mean±s.d. of a minimum of three independent experiments except for the right panel in E (two experiments). *P‐value 0.05 and **P‐value 0.01 as compared with cells treated in a same way, but transfected without siRNA.<graphic href="https://api.sourcedata.io/file.php?panel_id=4725"/></sd-panel></fig><fig id="1844"><title></title><label>Figure 5</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1844"/><sd-panel panel_id="4726">(A) Protein lysates from <sd-tag id="sdTag201" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag> cells left untreated (0) or treated with 50 ng/ml <sd-tag id="sdTag202" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TNF" ext_ids="P01375" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNF" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TNF</sd-tag>, 500 ng/ml <sd-tag id="sdTag203" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> (positive control) or 10 ng/ml <sd-tag id="sdTag198" source="sdapi" category="entity" entity_type="protein" role="intervention" text="IL‐1β" ext_ids="P01584" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="IL1B" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">IL‐1β</sd-tag> for indicated times were analysed by <sd-tag id="sdTag204" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag> for phosphorylated <sd-tag id="sdTag297" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="O00763///Q13085" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACB///ACACA" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag> (P‐<sd-tag id="sdTag298" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="O00763///Q13085" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACB///ACACA" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag>), phosphorylated <sd-tag id="sdTag302" source="sdapi" category="entity" entity_type="protein" role="assayed" text="IκB" ext_ids="Q9BYH8///Q8NI38///O00221///Q15653///P25963" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="NFKBIZ///NFKBID///NFKBIE///NFKBIB///NFKBIA" ext_tax_ids="9606///9606///9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">IκB</sd-tag> (P‐IκB) and <sd-tag id="sdTag205" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="GAPDH" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GAPDH</sd-tag> (loading control). The values show the P‐<sd-tag id="sdTag299" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="O00763///Q13085" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACB///ACACA" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag>/<sd-tag id="sdTag206" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="GAPDH" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GAPDH</sd-tag> and P‐IkB/<sd-tag id="sdTag207" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="GAPDH" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GAPDH</sd-tag> ratios as percentages of the ratios in untreated cells (left lanes) and are representative of two independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=4726"/></sd-panel><sd-panel panel_id="4727">(B, C) <sd-tag id="sdTag209" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="MCF10A" ext_ids="CVCL_0598" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="MCF-10A" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">MCF10A</sd-tag>-<sd-tag id="sdTag210" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag211" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> cells left untreated or treated with 500 ng/ml <sd-tag id="sdTag212" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag>, 50 ng/ml <sd-tag id="sdTag213" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TNF" ext_ids="P01375" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNF" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TNF</sd-tag> or 10 ng/ml <sd-tag id="sdTag214" source="sdapi" category="entity" entity_type="protein" role="intervention" text="IL‐1β" ext_ids="P01584" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="IL1B" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">IL‐1β</sd-tag> for 24 h were analysed for <sd-tag id="sdTag208" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9BXW4///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3C///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> translocation (B) and the DNA content (C). The values represent mean±s.d. for two independent experiments. **P‐value 0.01 and ***P‐value 0.001 as compared with untreated cells.<graphic href="https://api.sourcedata.io/file.php?panel_id=4727"/></sd-panel></fig><fig id="1845"><title></title><label>Figure 6</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1845"/><sd-panel panel_id="4728">(A) The lysates of <sd-tag id="sdTag218" source="sdapi" category="entity" entity_type="cell_line" role="component" text="hTERT–RPE1" ext_ids="CVCL_4388" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="hTERT-RPE1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">hTERT-RPE1</sd-tag> cells transfected with indicated siRNAs for 48 h and left untreated or treated with 500 ng/ml <sd-tag id="sdTag220" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for 2 h were analysed by <sd-tag id="sdTag222" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=4728"/></sd-panel><sd-panel panel_id="4729">(B) <sd-tag id="sdTag228" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="hTERT–RPE1" ext_ids="CVCL_4388" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="hTERT-RPE1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">hTERT-RPE1</sd-tag>-<sd-tag id="sdTag229" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag230" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> cells transfected with indicated siRNAs for 48 h were left untreated or treated with 500 ng/ml <sd-tag id="sdTag232" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for 24 h and analysed for <sd-tag id="sdTag234" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9BXW4///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3C///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> translocation (left) and sub‐G1 DNA content (middle) and <sd-tag id="sdTag235" source="sdapi" category="entity" entity_type="protein" role="assayed" text="AMPKα" ext_ids="P54646///Q13131" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="PRKAA2///PRKAA1" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AMPKα</sd-tag> expression (right).<graphic href="https://api.sourcedata.io/file.php?panel_id=4729"/></sd-panel><sd-panel panel_id="4730">(C) <sd-tag id="sdTag242" source="sdapi" category="entity" entity_type="cell_line" role="component" text="hTERT–RPE1" ext_ids="CVCL_4388" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="hTERT-RPE1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">hTERT-RPE1</sd-tag>-<sd-tag id="sdTag243" source="sdapi" category="entity" entity_type="protein" role="reporter" text="eGFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">eGFP</sd-tag>-<sd-tag id="sdTag244" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="440738///81631///84557" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="MAP1LC3C///MAP1LC3B///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> cells were transfected with indicated siRNAs for 48 h and analysed for the indicated mRNAs (top). After 48 h, cells were left untreated or treated with 500 ng/ml <sd-tag id="sdTag247" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> for 2 h, and analysed for P‐<sd-tag id="sdTag300" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="O00763///Q13085" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACB///ACACA" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag> and GAPDH (loading control) expression by <sd-tag id="sdTag251" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>. Similar results were obtained in two independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=4730"/></sd-panel><sd-panel panel_id="4731">(D) Cells transfected as described in (C) and left untreated or treated with 500 ng/ml <sd-tag id="sdTag258" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50591" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="TNFSF10" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag>, 10 μM <sd-tag id="sdTag259" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="ionomycin" ext_ids="CHEBI:63954" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ionomycin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ionomycin</sd-tag> or starved for <sd-tag id="sdTag260" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="amino acids" ext_ids="CHEBI:33709" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="amino acid" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">amino acids</sd-tag> and <sd-tag id="sdTag261" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> for 24 h were analysed for <sd-tag id="sdTag263" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9BXW4///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3C///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> translocation.<graphic href="https://api.sourcedata.io/file.php?panel_id=4731"/></sd-panel><sd-panel panel_id="4732">(E) Lysates of wild‐type (WT) <sd-tag id="sdTag279" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MEFs" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">MEFs</sd-tag> and <sd-tag id="sdTag281" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MEFs" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">MEFs</sd-tag> with an inactive <sd-tag id="sdTag280" source="sdapi" category="entity" entity_type="gene" role="intervention" text="TAK1" ext_ids="26409" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Map3k7" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">TAK1</sd-tag> knock‐in (<sd-tag id="sdTag282" source="sdapi" category="entity" entity_type="gene" role="intervention" text="TAK1" ext_ids="26409" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Map3k7" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">TAK1</sd-tag>Δ) treated with 500 ng/ml <sd-tag id="sdTag283" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50592" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Tnfsf10" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> were analysed by <sd-tag id="sdTag284" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>. Similar results were obtained in two independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=4732"/></sd-panel><sd-panel panel_id="4733">(F) WT and <sd-tag id="sdTag289" source="sdapi" category="entity" entity_type="gene" role="intervention" text="TAK1" ext_ids="26409" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Map3k7" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">TAK1</sd-tag>Δ <sd-tag id="sdTag290" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MEFs" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">MEFs</sd-tag> left untreated and treated with 250 or 500 ng/ml <sd-tag id="sdTag291" source="sdapi" category="entity" entity_type="protein" role="intervention" text="TRAIL" ext_ids="P50592" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Tnfsf10" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">TRAIL</sd-tag> were analysed for cell death by the LDH release assay. Similar results were obtained in two independent experiments (A, C and E). The values represent mean±s.d. for two (D) or a minimum of three independent experiments (B, F). *P‐value 0.05, **P‐value 0.01 and ***P‐value 0.001 as compared with cells treated in the same way, but transfected without siRNA (B, D) or the wild‐type cells (F).<graphic href="https://api.sourcedata.io/file.php?panel_id=4733"/></sd-panel></fig><fig id="1846"><title></title><label>Figure 7</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1846"/></fig></article>