token_classification/10-1038_emboj-2009-349.xml

<?xml version='1.0' encoding='UTF-8'?>
<article doi="10.1038/emboj.2009.349" abstract="Correction to:The EMBO Journal (2009) 28, 2244–2258. doi:10.1038/emboj.2009.159"><fig id="2440"><title></title><label>Figure 1</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2440"/><sd-panel panel_id="6885"><b>(B)</b> <sd-tag id="sdTag1" source="sdapi" category="entity" entity_type="cell_line" role="component" text="RAW 264.7 cells" ext_ids="CVCL_0493" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="RAW 264.7" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">RAW 264.7 cells</sd-tag> transfected for 48 h with control (sc), <sd-tag id="sdTag3" source="sdapi" category="entity" entity_type="gene" role="intervention" text="MTMR6" ext_ids="219135" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr6" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">MTMR6</sd-tag> or <sd-tag id="sdTag4" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA, were pretreated for 30 min with 100 nM <sd-tag id="sdTag6" source="sdapi" category="entity" entity_type="molecule" role="component" text="Bafilomycin A1" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Bafilomycin A1</sd-tag> (<sd-tag id="sdTag7" source="sdapi" category="entity" entity_type="molecule" role="component" text="BafA1" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">BafA1</sd-tag>), 10 μg/ml <sd-tag id="sdTag8" source="sdapi" category="entity" entity_type="molecule" role="component" text="E64d" ext_ids="CHEBI:30270" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="E64" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">E64d</sd-tag> and 10 μg/ml <sd-tag id="sdTag9" source="sdapi" category="entity" entity_type="molecule" role="component" text="pepstatin" ext_ids="CHEBI:7989" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="Pepstatin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">pepstatin</sd-tag>, then incubated for 30 min in full or starvation media in the presence of <sd-tag id="sdTag15" source="sdapi" category="entity" entity_type="molecule" role="component" text="BafA1" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">BafA1</sd-tag>, <sd-tag id="sdTag16" source="sdapi" category="entity" entity_type="molecule" role="component" text="E64d" ext_ids="CHEBI:30270" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="E64" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">E64d</sd-tag> and <sd-tag id="sdTag17" source="sdapi" category="entity" entity_type="molecule" role="component" text="pepstatin" ext_ids="CHEBI:7989" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="Pepstatin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">pepstatin</sd-tag>. Cells were lysed and analysed by <sd-tag id="sdTag442" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag> with anti-<sd-tag id="sdTag10" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> or anti‐<sd-tag id="sdTag11" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag>. Densitometric <span><sd-tag id="sdTag444" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>‐II</span>/<sd-tag id="sdTag18" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> ratios are shown underneath the blot. Graph: <span><sd-tag id="sdTag443" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>‐II</span>/<sd-tag id="sdTag19" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> ratio for <sd-tag id="sdTag20" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA in full medium is equal to or exceeds <span><sd-tag id="sdTag445" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>‐II</span>/<sd-tag id="sdTag21" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> ratio for control siRNA (sc) in starvation medium.<graphic href="https://api.sourcedata.io/file.php?panel_id=6885"/></sd-panel><sd-panel panel_id="6886"><b>(C, D)</b> <sd-tag id="sdTag24" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="RAW 264.7 cells" ext_ids="CVCL_0493" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="RAW 264.7" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">RAW 264.7 cells</sd-tag> were transfected for 36 h with control (scramble) or <sd-tag id="sdTag25" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA, transfected once more with corresponding siRNA and m<sd-tag id="sdTag36" source="sdapi" category="entity" entity_type="protein" role="reporter" text="RFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RFP</sd-tag>‐<sd-tag id="sdTag37" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag26" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="66734///67443" norm_text="" ext_dbs="NCBI gene///NCBI gene" in_caption="True" ext_names="Map1lc3a///Map1lc3b" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> DNA construct overnight and incubated for 2 h in full or starvation media. Cells were fixed and <sd-tag id="sdTag27" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> puncta analysed by <sd-tag id="sdTag33" source="sdapi" category="assay" entity_type="" role="" text="confocal fluorescence microscopy" ext_ids="BAO_0000453" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="confocal microscopy" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">confocal fluorescence microscopy</sd-tag>. (C) Representative confocal images of <sd-tag id="sdTag28" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="RAW 264.7 cells" ext_ids="CVCL_0493" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="RAW 264.7" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">RAW 264.7 cells</sd-tag> in full or starvation media after transfection with control (scramble) or <sd-tag id="sdTag29" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA (siJumpy). Red and yellow arrows indicate <sd-tag id="sdTag34" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag><sup>−</sup><sd-tag id="sdTag35" source="sdapi" category="entity" entity_type="protein" role="reporter" text="RFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RFP</sd-tag><sup>+</sup> and <sd-tag id="sdTag38" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag><sup>+</sup><sd-tag id="sdTag39" source="sdapi" category="entity" entity_type="protein" role="reporter" text="RFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RFP</sd-tag><sup>+</sup> puncta, respectively. (D) Quantitation of number of <sd-tag id="sdTag30" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> puncta per cell, total puncta per cell (<sd-tag id="sdTag40" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag><sup>+</sup><sd-tag id="sdTag41" source="sdapi" category="entity" entity_type="protein" role="reporter" text="RFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RFP</sd-tag><sup>+</sup> and <sd-tag id="sdTag42" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag><sup>−</sup><sd-tag id="sdTag43" source="sdapi" category="entity" entity_type="protein" role="reporter" text="RFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RFP</sd-tag><sup>+</sup> puncta) <sd-tag id="sdTag44" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag><sup>+</sup><sd-tag id="sdTag45" source="sdapi" category="entity" entity_type="protein" role="reporter" text="RFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RFP</sd-tag><sup>+</sup> puncta per cell and <sd-tag id="sdTag46" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag><sup>−</sup>RFP<sup>+</sup> puncta per cell. Data, mean±s.e.m. <i>n</i>=5 independent experiments, 30 cells per experiments. <sup>*</sup><i>P</i>0.05, <sup>**</sup><i>P</i>0.01, <sup>***</sup><i>P</i>0.001 (<i>t</i>‐test). Scale bars, 5 μm.<graphic href="https://api.sourcedata.io/file.php?panel_id=6886"/></sd-panel><sd-panel panel_id="6887"><b>(E, F)</b> <span><sd-tag id="sdTag448" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="C2C12" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">C2C12</sd-tag></span> myoblasts were transfected for 48 h with control (scramble) or <sd-tag id="sdTag50" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA, followed by a second transfection overnight with corresponding siRNA and m<sd-tag id="sdTag58" source="sdapi" category="entity" entity_type="protein" role="reporter" text="RFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RFP</sd-tag>‐<sd-tag id="sdTag51" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag52" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="67443///66734" norm_text="" ext_dbs="NCBI gene///NCBI gene" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> DNA construct. Cells were fixed and <sd-tag id="sdTag53" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> puncta were counted by <sd-tag id="sdTag54" source="sdapi" category="assay" entity_type="" role="" text="confocal fluorescence microscopy" ext_ids="BAO_0000453" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="confocal microscopy" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">confocal fluorescence microscopy</sd-tag>. (E) Representative confocal images of <sd-tag id="sdTag59" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465441920" parent-tag-id="475">C2C12</sd-pretag> cells</sd-tag> in full media after transfection with control (scramble) or <sd-tag id="sdTag55" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA (siJumpy). (F) Quantification of the number of <sd-tag id="sdTag56" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> puncta per cell. Data, mean±SEM for <i>n</i>=3 (independent experiments), 30 cells per experiments. <sup>*</sup><i>P</i> 0.05 (<i>t</i>-test). Scale bars, 10 μm.<graphic href="https://api.sourcedata.io/file.php?panel_id=6887"/></sd-panel><sd-panel panel_id="6888"><b>(G, H)</b> <sd-tag id="sdTag61" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465539430" parent-tag-id="475">C2C12</sd-pretag> cells</sd-tag> were transfected for 48 h with control (sc) (lanes 1, 2, 5, 6) or <sd-tag id="sdTag72" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Atg5" ext_ids="11793" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Atg5" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Atg5</sd-tag> siRNA (lanes 3, 4, 6, 7) followed by a second transfection for 48 h with same siRNA and control (lanes 1, 3, 5, 7) or <sd-tag id="sdTag64" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA (lanes 2, 4, 6, 8). Cells were incubated for 1 h with or without 100 nM <sd-tag id="sdTag75" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Baf A1" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Baf A1</sd-tag>, 10 μg/ml <sd-tag id="sdTag65" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="E64d" ext_ids="CHEBI:30270" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="E64" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">E64d</sd-tag> and 10 μg/ml <sd-tag id="sdTag66" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="pepstatin" ext_ids="CHEBI:7989" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="Pepstatin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">pepstatin</sd-tag> in full media, lysed and analysed by <sd-tag id="sdTag78" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag> with anti‐<sd-tag id="sdTag67" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> or anti‐<sd-tag id="sdTag68" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> (G). (H) Densitometric <span><sd-tag id="sdTag449" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>‐II</span>/<sd-tag id="sdTag70" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> ratios for samples treated with <sd-tag id="sdTag71" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="BafA1" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">BafA1</sd-tag> and protease inhibitors from G (lanes 5-8). Inset shows <sd-tag id="sdTag79" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg5" ext_ids="Q99J83" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Atg5" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Atg5</sd-tag> knock‐down by <sd-tag id="sdTag76" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=6888"/></sd-panel></fig><fig id="2441"><title></title><label>Figure 2</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2441"/><sd-panel panel_id="6889"><b>(A)</b> Proteolysis of long‐lived proteins in <sd-tag id="sdTag80" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 myoblasts" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">C2C12 myoblasts</sd-tag>. <sd-tag id="sdTag81" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">C2C12 cells</sd-tag> were transfected with control (scramble) or <sd-tag id="sdTag82" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA (siJumpy), labelled overnight in media containing <sd-tag id="sdTag84" source="sdapi" category="entity" entity_type="molecule" role="reporter" text="[3H] leucine" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">[<sup>3</sup>H] leucine</sd-tag>, washed, incubated for 2 h in complete media (containing cold leucine) and incubated for 4 h in full or starvation media. <sd-tag id="sdTag86" source="sdapi" category="entity" entity_type="molecule" role="reporter" text="Leucine" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Leucine</sd-tag> release was calculated from radioactivity in the tricarboxylic acid‐soluble form relative to total cell radioactivity. Results shown represent mean±s.e.m. for combined data from three independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=6889"/></sd-panel><sd-panel panel_id="6890"><b>(B)</b> Quantitation of <sd-tag id="sdTag93" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> protein levels. <sd-tag id="sdTag88" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465041970" parent-tag-id="475">C2C12</sd-pretag> cells</sd-tag> were transfected for 48 h with control (sc) or <sd-tag id="sdTag90" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA and <sd-tag id="sdTag92" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> levels analysed by <sd-tag id="sdTag91" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>, quantitated by densitometry and represented as a percentage of control. Data are mean±s.e.m. (<i>n</i>=4 independent experiments).<graphic href="https://api.sourcedata.io/file.php?panel_id=6890"/></sd-panel><sd-panel panel_id="6891"><b>(C)</b> <sd-tag id="sdTag95" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465456510" parent-tag-id="475">C2C12</sd-pretag> cells</sd-tag> were transfected for 48 h with control (sc) or <sd-tag id="sdTag97" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA, incubated for 4 h with or without 100 nM <sd-tag id="sdTag99" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Bafilomycin A1" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Bafilomycin A1</sd-tag> in full media, lysed, probed for endogenous <sd-tag id="sdTag100" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> and <sd-tag id="sdTag101" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> by <sd-tag id="sdTag102" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag> and percentage of <sd-tag id="sdTag103" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> were quantitated (mean±s.e.m., <i>n</i>=3).<graphic href="https://api.sourcedata.io/file.php?panel_id=6891"/></sd-panel><sd-panel panel_id="6892"><b>(D)</b> <sd-tag id="sdTag105" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465186720" parent-tag-id="475">C2C12</sd-pretag> cells</sd-tag> were transfected for 48 h with control (sc) (lanes 1, 2) or <sd-tag id="sdTag113" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin" ext_ids="56208" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Becn1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin</sd-tag> siRNA (lanes 3, 4) followed by a second transfection for 48 h with same siRNA and control (lanes 1, 3) or <sd-tag id="sdTag108" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA (lanes 2, 4). Cells were lysed, probed for <sd-tag id="sdTag109" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag>, <sd-tag id="sdTag114" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin" ext_ids="O88597" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Becn1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Beclin</sd-tag> and <sd-tag id="sdTag110" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> by <sd-tag id="sdTag111" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag> and percentage of <sd-tag id="sdTag112" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> were quantitated (mean±s.e.m., <i>n</i>=5).<graphic href="https://api.sourcedata.io/file.php?panel_id=6892"/></sd-panel><sd-panel panel_id="6893"><b>(E-M)</b> <sd-tag id="sdTag116" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465425130" parent-tag-id="475">C2C12</sd-pretag> cells</sd-tag> were transfected for 48 h with <span><sd-tag id="sdTag451" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag></span>, <sd-tag id="sdTag119" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag120" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> (<sd-tag id="sdTag121" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag>), <sd-tag id="sdTag122" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag134" source="sdapi" category="entity" entity_type="gene" role="intervention" text="MTM1" ext_ids="17772" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">MTM1</sd-tag> (<sd-tag id="sdTag136" source="sdapi" category="entity" entity_type="protein" role="assayed" text="MTM1" ext_ids="Q9Z2C5" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">MTM1</sd-tag>) or <sd-tag id="sdTag123" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag135" source="sdapi" category="entity" entity_type="gene" role="intervention" text="MTMR2" ext_ids="77116" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr2" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">MTMR2</sd-tag> (<sd-tag id="sdTag137" source="sdapi" category="entity" entity_type="protein" role="assayed" text="MTMR2" ext_ids="Q9Z2D1" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr2" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">MTMR2</sd-tag><span>), fixed and <sd-pretag id="sdPretag14395464273220" parent-tag-id="473">immunostained</sd-pretag> with anti‐</span><sd-tag id="sdTag124" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> antibody (red). <sd-tag id="sdTag125" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> puncta were quantitated by <sd-tag id="sdTag126" source="sdapi" category="assay" entity_type="" role="" text="confocal fluorescence microscopy" ext_ids="BAO_0000453" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="confocal microscopy" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">confocal fluorescence microscopy</sd-tag>. Representative confocal images of <sd-tag id="sdTag127" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> (E), <sd-tag id="sdTag128" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag129" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> (F), <sd-tag id="sdTag130" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag138" source="sdapi" category="entity" entity_type="gene" role="intervention" text="MTM1" ext_ids="17772" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">MTM1</sd-tag> (G), <sd-tag id="sdTag131" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag139" source="sdapi" category="entity" entity_type="gene" role="intervention" text="MTMR2" ext_ids="77116" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr2" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">MTMR2</sd-tag> <span>(H) transfected cells, <sd-pretag id="sdPretag14395464247310" parent-tag-id="473">immunostained</sd-pretag> for</span> <sd-tag id="sdTag132" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> (I), (J), (K) and (L), respectively. Scale bars, 5 μm. White lines represent outline of the cells. Quantitation of <sd-tag id="sdTag133" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> puncta per cell (M). Bars, SEM (<i>n</i>=3 independent experiments, with an average of 30 cells per experiments).<graphic href="https://api.sourcedata.io/file.php?panel_id=6893"/></sd-panel><sd-panel panel_id="6894"><b>(N, O)</b> <sd-tag id="sdTag140" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465762770" parent-tag-id="475">C2C12</sd-pretag> cells</sd-tag> were transfected for 48 h with <sd-pretag id="sdPretag14395452149790" parent-tag-id="464">GFP</sd-pretag>, <sd-tag id="sdTag143" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag144" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> (<sd-tag id="sdTag145" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag>), <sd-tag id="sdTag146" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag147" source="sdapi" category="entity" entity_type="gene" role="intervention" text="MTM1" ext_ids="17772" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">MTM1</sd-tag> (<sd-tag id="sdTag157" source="sdapi" category="entity" entity_type="protein" role="assayed" text="MTM1" ext_ids="Q9Z2C5" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">MTM1</sd-tag>) or <sd-tag id="sdTag158" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag159" source="sdapi" category="entity" entity_type="gene" role="intervention" text="MTMR2" ext_ids="77116" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr2" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">MTMR2</sd-tag> (<sd-tag id="sdTag160" source="sdapi" category="entity" entity_type="protein" role="assayed" text="MTMR2" ext_ids="Q9Z2D1" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr2" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">MTMR2</sd-tag>), lysed, analysed for <sd-tag id="sdTag161" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag>, <sd-tag id="sdTag162" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> and <sd-tag id="sdTag163" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> by <sd-tag id="sdTag164" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag> (N) and percentage of <sd-tag id="sdTag165" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> were quantitated (mean±s.e.m., <i>n</i>=3) (O).<graphic href="https://api.sourcedata.io/file.php?panel_id=6894"/></sd-panel><sd-panel panel_id="6895"><b>(P, Q)</b> <sd-tag id="sdTag166" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465319390" parent-tag-id="475">C2C12</sd-pretag> cells</sd-tag> were transfected for 48 h with <sd-pretag id="sdPretag14395451425010" parent-tag-id="464">GFP</sd-pretag> or <sd-tag id="sdTag169" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag170" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> (<sd-tag id="sdTag171" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag>), incubated with or without 100 nM <sd-tag id="sdTag172" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Bafilomycin A1" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Bafilomycin A1</sd-tag> (<sd-tag id="sdTag173" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="BafA1" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">BafA1</sd-tag>) for 4 h, lysed, analysed for <sd-tag id="sdTag175" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag>, <sd-tag id="sdTag176" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> and <sd-tag id="sdTag177" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> by <sd-tag id="sdTag178" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag> (P) and percentage of <sd-tag id="sdTag179" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> were quantitated (mean±s.e.m., <i>n</i>=4) (Q). <sup>*</sup><i>P</i>0.05, <sup>***</sup><i>P</i>0.001, †ns (<i>t</i>‐test).<graphic href="https://api.sourcedata.io/file.php?panel_id=6895"/></sd-panel></fig><fig id="2442"><title></title><label>Figure 3</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2442"/><sd-panel panel_id="6896"><b>(A)</b> Transient association of <sd-tag id="sdTag180" source="sdapi" category="entity" entity_type="gene" role="component" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> WT with <sd-tag id="sdTag181" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q91VR7///Q9CQV6" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3a///Map1lc3b" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag><sup>+</sup> organelles. Time lapse sequence of <sd-tag id="sdTag182" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 myoblasts" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465542310" parent-tag-id="475" class="component cell">C2C12</sd-pretag> myoblasts</sd-tag> transfected for 24 h with <sd-tag id="sdTag183" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag184" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag> WT and <sd-tag id="sdTag196" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Cherry" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Cherry</sd-tag>‐<sd-tag id="sdTag185" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q91VR7///Q9CQV6" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3a///Map1lc3b" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> <span>and analysed by <sd-tag id="sdTag454" source="sdapi" category="assay" entity_type="" role="" text="live confocal microscopy" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">live confocal microscopy</sd-tag> in a 5LIVE Zeiss microscope.</span> <sd-tag id="sdTag195" source="sdapi" category="entity" entity_type="undefined" role="component" text="EBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">EBSS</sd-tag> was added to the cells and <i>z</i>‐stacks collected at 3‐min intervals for a total of 45 min. The collected images were processed to generate a maximum projection (collapsing a 3D image into an x-y projection) for each time point. Arrows indicate colocalization of <sd-tag id="sdTag186" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag187" source="sdapi" category="entity" entity_type="gene" role="component" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> WT with <sd-tag id="sdTag188" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q91VR7///Q9CQV6" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3a///Map1lc3b" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>. Graph: Plots of relative fluorescence intensity (<sd-tag id="sdTag189" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> fluorescence pixel intensity) over time of <sd-tag id="sdTag190" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag191" source="sdapi" category="entity" entity_type="gene" role="component" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> WT colocalizing with <sd-tag id="sdTag197" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Cherry" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Cherry</sd-tag>‐<sd-tag id="sdTag192" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="67443///66734" norm_text="" ext_dbs="NCBI gene///NCBI gene" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag><sup>+</sup>‐positive puncta indicated by arrows.<graphic href="https://api.sourcedata.io/file.php?panel_id=6896"/></sd-panel><sd-panel panel_id="6897"><b>(B-I)</b> Association of <sd-tag id="sdTag198" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS mutant with <sd-tag id="sdTag434" source="sdapi" category="entity" entity_type="subcellular" role="component" text="autophagic organelles" ext_ids="GO:0005776" norm_text="" ext_dbs="Gene Ontology" in_caption="True" ext_names="autophagic vacuole" ext_tax_ids="" ext_tax_names="" ext_urls="http://amigo.geneontology.org/amigo/term/">autophagic organelles</sd-tag>. <sd-tag id="sdTag456" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">C2C12</sd-tag> cells were transfected for 24 h with <sd-tag id="sdTag201" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag457" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> C330S (<sd-tag id="sdTag458" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS) and <sd-tag id="sdTag219" source="sdapi" category="entity" entity_type="protein" role="reporter" text="tdTomato" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">tdTomato</sd-tag>‐<sd-tag id="sdTag204" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (<sd-tag id="sdTag205" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag><span>), fixed and <sd-tag id="sdTag495" source="sdapi" category="assay" entity_type="" role="" text="immunostained" ext_ids="BAO_0002426" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunofluorescent labeling" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunostained</sd-tag> with anti‐</span>G58K (<sd-tag id="sdTag220" source="sdapi" category="assay" entity_type="" role="" text="Alexa 648" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Alexa 648</sd-tag>‐labelled secondary antibody). <sd-tag id="sdTag206" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag> CS (green), <sd-tag id="sdTag207" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (red) and G58K (white in D, H or blue in E, I). Boxed areas (B-E) are shown at higher magnification in the corresponding panel below (F-I). Scale bars, 2 μm. White arrows indicate colocalization between <sd-tag id="sdTag208" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS, <sd-tag id="sdTag209" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> but not G58K, blue arrow indicates colocalization between <sd-tag id="sdTag459" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS and G58K but not <sd-tag id="sdTag211" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="67443///66734" norm_text="" ext_dbs="NCBI gene///NCBI gene" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=6897"/></sd-panel></fig><fig id="2443"><title></title><label>Figure 4</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2443"/><sd-panel panel_id="6898"><b>(A-H)</b> <sd-tag id="sdTag460" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">C2C12</sd-tag> cells were transfected for 24 h with <sd-tag id="sdTag223" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag461" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> C330S (<sd-tag id="sdTag462" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS) and <sd-tag id="sdTag226" source="sdapi" category="entity" entity_type="protein" role="reporter" text="tdTomato" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">tdTomato</sd-tag>‐<sd-tag id="sdTag227" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="67443///66734" norm_text="" ext_dbs="NCBI gene///NCBI gene" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> (<sd-tag id="sdTag228" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag><span>), fixed and <sd-pretag id="sdPretag14395464070440" parent-tag-id="473">immunostained</sd-pretag> with anti‐</span><sd-tag id="sdTag242" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg16" ext_ids="Q8C0J2///Q6KAU8" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Atg16l1///Atg16l2" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">Atg16</sd-tag> (<sd-tag id="sdTag229" source="sdapi" category="assay" entity_type="" role="" text="Alexa 648" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Alexa 648</sd-tag>‐labelled secondary antibody). <sd-tag id="sdTag230" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS (green), <sd-tag id="sdTag231" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (red) and <sd-tag id="sdTag243" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg16" ext_ids="Q8C0J2///Q6KAU8" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Atg16l1///Atg16l2" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">Atg16</sd-tag> (white in B and F or blue in D and H). Boxed areas (A-D) are shown at higher magnification in the corresponding panel below (E-H). White arrows indicate colocalization among <sd-tag id="sdTag463" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS, <sd-tag id="sdTag233" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> and <sd-tag id="sdTag241" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg16" ext_ids="Q8C0J2///Q6KAU8" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Atg16l1///Atg16l2" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">Atg16</sd-tag>, yellow arrow indicates colocalization between <sd-tag id="sdTag234" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS and <sd-tag id="sdTag235" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> but not <sd-tag id="sdTag244" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg16" ext_ids="Q8C0J2///Q6KAU8" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Atg16l1///Atg16l2" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">Atg16</sd-tag>, blue arrow indicates colocalization between <sd-tag id="sdTag236" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag> CS and <sd-tag id="sdTag245" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg16" ext_ids="Q8C0J2///Q6KAU8" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Atg16l1///Atg16l2" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">Atg16</sd-tag> but not <sd-tag id="sdTag237" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=6898"/></sd-panel><sd-panel panel_id="6899"><b>(I, J)</b> <sd-tag id="sdTag248" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465314410" parent-tag-id="475">C2C12</sd-pretag> cells</sd-tag> were transfected for 24 h with <span><sd-tag id="sdTag464" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag></span> <span>(I), fixed and <sd-pretag id="sdPretag14395464514400" parent-tag-id="473">immunostained</sd-pretag> with anti‐</span><sd-tag id="sdTag251" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg16" ext_ids="Q8C0J2///Q6KAU8" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Atg16l1///Atg16l2" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">Atg16</sd-tag> (J).<graphic href="https://api.sourcedata.io/file.php?panel_id=6899"/></sd-panel><sd-panel panel_id="6900"><b>(K-R)</b> <sd-tag id="sdTag466" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">C2C12</sd-tag> cells were transfected for 24 h with <sd-tag id="sdTag254" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag467" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> C330S (<sd-tag id="sdTag468" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS) and <sd-tag id="sdTag257" source="sdapi" category="entity" entity_type="protein" role="reporter" text="tdTomato" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">tdTomato</sd-tag>‐<sd-tag id="sdTag258" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="67443///66734" norm_text="" ext_dbs="NCBI gene///NCBI gene" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> (<sd-tag id="sdTag259" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag><span>), fixed and <sd-pretag id="sdPretag14395464068600" parent-tag-id="473">immunostained</sd-pretag> with anti‐</span><sd-tag id="sdTag272" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg12" ext_ids="Q9CQY1" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Atg12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Atg12</sd-tag>(M) antibody. <sd-tag id="sdTag260" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS (green), <sd-tag id="sdTag261" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (red) and <sd-tag id="sdTag273" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg12" ext_ids="Q9CQY1" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Atg12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Atg12</sd-tag> (white in L, P or blue in N, R). Boxed areas (K-N) are shown at higher magnification in the corresponding panel below (O-R). White arrows indicate colocalization among <sd-tag id="sdTag262" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS, <sd-tag id="sdTag263" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> and <sd-tag id="sdTag271" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg12" ext_ids="Q9CQY1" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Atg12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Atg12</sd-tag><span>, yellow arrow indicates colocalization between <sd-tag id="sdTag469" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag> CS and</span> <sd-tag id="sdTag265" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> but not <sd-tag id="sdTag274" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg12" ext_ids="Q9CQY1" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Atg12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Atg12</sd-tag>, blue arrow indicates colocalization between <span><sd-tag id="sdTag465" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag></span> CS and <sd-tag id="sdTag275" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg12" ext_ids="Q9CQY1" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Atg12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Atg12</sd-tag> but not <sd-tag id="sdTag267" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=6900"/></sd-panel><sd-panel panel_id="29638"><p>(<strong>S</strong>-<strong>ZA</strong>) C2C12 cells were transfected for 24 h with <sd-tag id="sdTag501" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS and <sd-tag id="sdTag502" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Cherry" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Cherry</sd-tag>‐<sd-tag id="sdTag503" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (<sd-tag id="sdTag504" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>), fixed and <sd-tag id="sdTag505" source="sdapi" category="assay" entity_type="" role="" text="immunostained" ext_ids="BAO_0002426" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunofluorescent labeling" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunostained</sd-tag> with anti‐<sd-tag id="sdTag506" source="sdapi" category="entity" entity_type="protein" role="assayed" text="lamp1" ext_ids="P11438" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Lamp1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">lamp1</sd-tag>. <sd-tag id="sdTag507" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS (green), <sd-tag id="sdTag508" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (red) and <sd-tag id="sdTag509" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Lamp1" ext_ids="P11438" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Lamp1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Lamp1</sd-tag> (white in T, X or blue in V, ZA). Boxed areas (S-V) are shown at higher magnification in the corresponding panel below (W-ZA). Yellow arrows indicate colocalization between <sd-tag id="sdTag510" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS and <sd-tag id="sdTag511" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> but not <sd-tag id="sdTag512" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Lamp1" ext_ids="P11438" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Lamp1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Lamp1</sd-tag>. Scale bars, 2 μm.</p><graphic href="https://api.sourcedata.io/file.php?panel_id=29638"/></sd-panel></fig><fig id="2444"><title></title><label>Figure 5</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2444"/><sd-panel panel_id="6902"><b>(A-C)</b> <sd-tag id="sdTag305" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">C2C12</sd-tag> were transfected for 48 h with <sd-tag id="sdTag296" source="sdapi" category="entity" entity_type="gene" role="component" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA, followed by a second transfection overnight with <sd-tag id="sdTag297" source="sdapi" category="entity" entity_type="gene" role="component" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA and <sd-tag id="sdTag298" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag306" source="sdapi" category="entity" entity_type="gene" role="component" text="WIPI‐1" ext_ids="52639" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Wipi1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">WIPI‐1</sd-tag>, incubated for 4 h in starvation media, fixed and immunostained with anti‐<sd-tag id="sdTag300" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg16" ext_ids="Q8C0J2///Q6KAU8" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Atg16l1///Atg16l2" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">Atg16</sd-tag> antibody (red). <sd-tag id="sdTag301" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag307" source="sdapi" category="entity" entity_type="protein" role="assayed" text="WIPI1" ext_ids="Q8R3E3" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Wipi1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">WIPI1</sd-tag>‐transfected cell (A), endogenous <sd-tag id="sdTag302" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg16" ext_ids="Q8C0J2///Q6KAU8" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Atg16l1///Atg16l2" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">Atg16</sd-tag> <sd-tag id="sdTag310" source="sdapi" category="assay" entity_type="" role="" text="immunostaining" ext_ids="BAO_0002426" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunofluorescent labeling" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunostaining</sd-tag> (B), colocalization of <sd-tag id="sdTag303" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag308" source="sdapi" category="entity" entity_type="gene" role="component" text="WIPI‐1" ext_ids="52639" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Wipi1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">WIPI‐1</sd-tag> and <sd-tag id="sdTag304" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg16" ext_ids="Q8C0J2///Q6KAU8" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Atg16l1///Atg16l2" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">Atg16</sd-tag> is indicated in yellow in merge image (C). Boxed areas are shown at higher magnification as inset. Scale bars, 10 and 5 μm in insets.<graphic href="https://api.sourcedata.io/file.php?panel_id=6902"/></sd-panel><sd-panel panel_id="6903"><b>(D-I)</b> <sd-tag id="sdTag311" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12 cells" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:"><sd-pretag id="sdPretag14395465418360" parent-tag-id="475">C2C12</sd-pretag> cells</sd-tag> were transfected for 48 h with control (scramble) (D, E) or <sd-tag id="sdTag313" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> siRNA (F-H), followed by a second transfection overnight with corresponding siRNA and <sd-tag id="sdTag314" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag315" source="sdapi" category="entity" entity_type="gene" role="component" text="WIPI‐1" ext_ids="52639" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Wipi1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">WIPI‐1</sd-tag>. Cells were incubated for 4 h in full (D, G) or starvation media (E, F, H) in presence (F) or absence (E-H) of 100 nM <sd-tag id="sdTag319" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="wortmannin" ext_ids="CHEBI:52289" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="wortmannin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">wortmannin</sd-tag>, fixed and analysed by <sd-tag id="sdTag317" source="sdapi" category="assay" entity_type="" role="" text="confocal fluorescence microscopy" ext_ids="BAO_0000453" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="confocal microscopy" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">confocal fluorescence microscopy</sd-tag>. Quantitation of <sd-tag id="sdTag318" source="sdapi" category="entity" entity_type="protein" role="assayed" text="WIPI‐1" ext_ids="Q8R3E3" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Wipi1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">WIPI‐1</sd-tag> puncta per cell (I). Bars, s.e.m. (<i>n</i>=3 independent experiments, with an average of 30 cells per experiment). <sup>*</sup><i>P</i>0.05, <sup>**</sup><i>P</i>0.01 (<i>t</i>‐test).<graphic href="https://api.sourcedata.io/file.php?panel_id=6903"/></sd-panel></fig><fig id="2445"><title></title><label>Figure 6</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2445"/><sd-panel panel_id="6904"><b>(A-T)</b> <sd-tag id="sdTag475" source="sdapi" category="entity" entity_type="cell_line" role="component" text="C2C12" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">C2C12</sd-tag> cells were transfected for 24 h with GFP, <sd-tag id="sdTag329" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag330" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> WT or <sd-tag id="sdTag331" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>‐<sd-tag id="sdTag476" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> C330S (<sd-tag id="sdTag477" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS) and <sd-tag id="sdTag439" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Tomato" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Tomato</sd-tag>‐<sd-tag id="sdTag332" source="sdapi" category="entity" entity_type="gene" role="component" text="LC3" ext_ids="67443///66734" norm_text="" ext_dbs="NCBI gene///NCBI gene" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">LC3</sd-tag> (<sd-tag id="sdTag333" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>) and <sd-tag id="sdTag325" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>‐<sd-tag id="sdTag326" source="sdapi" category="entity" entity_type="gene" role="component" text="Atg9" ext_ids="245860" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Atg9a" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Atg9</sd-tag>, fixed and <sd-tag id="sdTag478" source="sdapi" category="assay" entity_type="" role="" text="immunostained" ext_ids="BAO_0002426" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunofluorescent labeling" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunostained</sd-tag> with anti‐<sd-tag id="sdTag334" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>. <sd-tag id="sdTag335" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> (I), <sd-tag id="sdTag336" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag> WT (M), <sd-tag id="sdTag479" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS (green) (A, E, Q), <sd-tag id="sdTag338" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (red) (C, G, J, N, R) and <sd-tag id="sdTag339" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Atg9" ext_ids="Q68FE2///Q6EBV9" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Atg9a///Atg9b" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">Atg9</sd-tag> (white in B, F, K, O, S or blue in D, H, I, P, T). Boxed areas (A-D) are shown at higher magnification in the corresponding panel below (E-H). White arrows indicate colocalization between <sd-tag id="sdTag340" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS, <sd-tag id="sdTag341" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> and <sd-tag id="sdTag342" source="sdapi" category="entity" entity_type="gene" role="component" text="Atg9" ext_ids="245860" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Atg9a" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Atg9</sd-tag>, blue arrow indicates colocalization between <sd-tag id="sdTag343" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS and <sd-tag id="sdTag344" source="sdapi" category="entity" entity_type="gene" role="component" text="Atg9" ext_ids="245860" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Atg9a" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Atg9</sd-tag> but not <sd-tag id="sdTag345" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> and yellow arrow indicates colocalization between <sd-tag id="sdTag346" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> CS and <sd-tag id="sdTag347" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> but not <sd-tag id="sdTag348" source="sdapi" category="entity" entity_type="gene" role="component" text="Atg9" ext_ids="245860" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Atg9a" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Atg9</sd-tag>. White boxes (I-T) show <sd-tag id="sdTag349" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> puncta location. Scale bars, 5 μm.<graphic href="https://api.sourcedata.io/file.php?panel_id=6904"/></sd-panel><sd-panel panel_id="6905"><b>(U)</b> Percentage of <sd-tag id="sdTag352" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> puncta colocalizing with <sd-tag id="sdTag353" source="sdapi" category="entity" entity_type="gene" role="component" text="Atg9" ext_ids="245860" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Atg9a" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Atg9</sd-tag>. <i>n</i>, number of puncta counted.<graphic href="https://api.sourcedata.io/file.php?panel_id=6905"/></sd-panel></fig><fig id="2446"><title></title><label>Figure 7</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2446"/><sd-panel panel_id="6906"><b>(A-K)</b> <sd-tag id="sdTag480" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="C2C12" ext_ids="CVCL_0188" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="C2C12" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">C2C12</sd-tag> <span>cells were transfected for 48 h with <sd-pretag id="sdPretag14961408039910" parent-tag-id="514">YFP</sd-pretag>,</span> <sd-tag id="sdTag382" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag><span>‐</span><sd-tag id="sdTag383" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>WT (</span><sd-tag id="sdTag384" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>WT),</span> <sd-tag id="sdTag369" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag><span>‐</span><sd-tag id="sdTag481" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>C330S (</span><sd-tag id="sdTag482" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>CS),</span> <sd-tag id="sdTag387" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag><span>‐</span><sd-tag id="sdTag483" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>Y462C (</span><sd-tag id="sdTag484" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>YC) or <sd-tag id="sdTag514">YFP</sd-tag>‐</span><sd-tag id="sdTag485" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>R336Q (</span><sd-tag id="sdTag486" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>RQ), fixed and immunostained with anti‐</span><sd-tag id="sdTag403" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> <span>antibody (red).</span> <sd-tag id="sdTag394" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> <span>puncta were quantitated by</span> <sd-tag id="sdTag395" source="sdapi" category="assay" entity_type="" role="" text="confocal fluorescence microscopy" ext_ids="BAO_0000453" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="confocal microscopy" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">confocal fluorescence microscopy</sd-tag><span>. Representative confocal images of</span> <sd-tag id="sdTag396" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag> <span>(A),</span> <sd-tag id="sdTag397" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag><span>‐</span><sd-tag id="sdTag377" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag> <span>WT (B),</span> <sd-tag id="sdTag398" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag><span>‐</span><sd-tag id="sdTag488" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>C330S (C),</span> <sd-tag id="sdTag399" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag><span>‐</span><sd-tag id="sdTag489" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>Y462C (D) and</span> <sd-tag id="sdTag400" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag><span>‐</span><sd-tag id="sdTag490" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="97287" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> <span>R336Q (E) transfected cells, immunostained for</span> <sd-tag id="sdTag401" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> <span>(F), (G), (H), (I) and (J), respectively. Scale bars, 5 μm. Quantitation of</span> <sd-tag id="sdTag402" source="sdapi" category="entity" entity_type="protein" role="assayed" text="p62" ext_ids="Q64337" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">p62</sd-tag> <span>puncta per cell (K). Bars, s.e.m. (</span><i>n</i><span>=3 independent experiments, 30 cells per experiments).</span> <sup>*</sup><i>P</i><span>0.05 (</span><i>t</i><span>‐test),</span> <i>ns</i><span>: nonsignificant.</span><graphic href="https://api.sourcedata.io/file.php?panel_id=6906"/></sd-panel><sd-panel panel_id="6907"><b>(L)</b> <sd-tag id="sdTag407" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa cells" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa cells</sd-tag> were transfected for 24 h with <sd-pretag id="sdPretag14961407194930" parent-tag-id="514">YFP</sd-pretag>, <sd-tag id="sdTag412" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag>‐<sd-tag id="sdTag413" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="64419" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="MTMR14" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> WT (<sd-tag id="sdTag414" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="64419" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="MTMR14" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> WT) or <sd-tag id="sdTag415" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag>‐<span><sd-tag id="sdTag492" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Jumpy" ext_ids="Q8VEL2" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Mtmr14" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Jumpy</sd-tag></span> R336Q (<sd-tag id="sdTag494" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Jumpy" ext_ids="64419" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="MTMR14" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Jumpy</sd-tag> RQ), incubated with or without 50 ng/μl <sd-tag id="sdTag426" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="rapamycin" ext_ids="CHEBI:9168" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="sirolimus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">rapamycin</sd-tag> (<sd-tag id="sdTag416" source="sdapi" category="entity" entity_type="molecule" role="component" text="BafA1" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">BafA1</sd-tag> (100 nM) was present in both control and <sd-tag id="sdTag424" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="rapamycin" ext_ids="CHEBI:9168" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="sirolimus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">rapamycin</sd-tag> treated cells) for 2 h, lysed and analysed for <sd-tag id="sdTag418" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>, <sd-tag id="sdTag419" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag> and <sd-tag id="sdTag420" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> by <sd-tag id="sdTag421" source="sdapi" category="assay" entity_type="" role="" text="immunoblotting" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&amp;conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotting</sd-tag>. Densitometric <span><sd-tag id="sdTag493" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9GZQ8///Q9BXW4" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3B///MAP1LC3C" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag></span>‐II/<sd-tag id="sdTag423" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">actin</sd-tag> ratios are shown underneath the blot.<graphic href="https://api.sourcedata.io/file.php?panel_id=6907"/></sd-panel></fig></article>