Figure 6 ( A ) Primary root length of Col - 0 wild type plants or eir1 - 1 mutant plants was measured using the ImageJ program 3 days after 5 mM KNO3 or KCl treatments . Tukey box plot show results from 3 independent biological replicates per experimental condition ( n = 10 - 15 roots each replicate ) . The box plot shows the data within the interquartile range ( 25th and 75th percentiles ) and a solid black line represents the median . Whiskers show maximum and minimum values no further than 1 . 5x IQR ( interquartile range ) . Outlier data are plotted individually . Asterisk indicates statistically significant difference between analyzed by unpaired , two - tailed and assuming equal variance t - test ( * * p < 0 . 01 ) . ( B ) Number of initiating and emerging lateral roots of Col - 0 or eir1 - 1 mutant plants were counted using light microscopy 3 days after 5 mM KNO3 or KCl treatments . Tukey box plot show results from 3 independent biological replicates per experimental condition ( n = 10 - 15 roots each replicate ) . The box plot shows the data within the interquartile range ( 25th and 75th percentiles ) and a solid black line represents the median . Whiskers show maximum and minimum values no further than 1 . 5x IQR ( interquartile range ) . Asterisk indicates statistically significant difference between analyzed by unpaired , two - tailed and assuming equal variance t - test ( * * p < 0 . 01 ) .

O O B-PANEL_START O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B-PANEL_START O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O

Figure 6(A) Primary root length of Col-0 wild type plants or eir1-1 mutant plants was measured using the ImageJ program 3 days after 5 mM KNO3 or KCl treatments. Tukey box plot show results from 3 independent biological replicates per experimental condition (n = 10-15 roots each replicate). The box plot shows the data within the interquartile range (25th and 75th percentiles) and a solid black line represents the median. Whiskers show maximum and minimum values no further than 1.5x IQR (interquartile range). Outlier data are plotted individually. Asterisk indicates statistically significant difference between analyzed by unpaired, two-tailed and assuming equal variance t-test (** p < 0.01).(B) Number of initiating and emerging lateral roots of Col-0 or eir1-1 mutant plants were counted using light microscopy 3 days after 5 mM KNO3 or KCl treatments. Tukey box plot show results from 3 independent biological replicates per experimental condition (n = 10-15 roots each replicate). The box plot shows the data within the interquartile range (25th and 75th percentiles) and a solid black line represents the median. Whiskers show maximum and minimum values no further than 1.5x IQR (interquartile range). Asterisk indicates statistically significant difference between analyzed by unpaired, two-tailed and assuming equal variance t-test (** p < 0.01).


Figure 7 ( A ) Primary root length of the different genotypes was measured using the ImageJ program 3 days after 5 mM KNO3 or KCl treatments . Tukey box plot show results from 3 independent biological replicates per experimental condition ( n = 8 - 10 roots each replicate ) . The box plot shows the data within the interquartile range ( 25th and 75th percentiles ) and a solid black line represents the median . Whiskers show maximum and minimum values no further than 1 . 5x IQR ( interquartile range ) . Outlier data are plotted individually . Asterisk indicate statistically significant difference between means analyzed by unpaired , two - tailed and assuming equal variance t - test ( * p < 0 . 05 , * * p < 0 . 01 ) . ( B ) Number of initiating and emerging lateral roots for all genotypes were counted using light microscopy 3 days after 5 mM KNO3 or KCl treatments . Tukey box plot show results from 3 independent biological replicates per experimental condition ( n = 8 - 10 roots each replicate ) . The box plot shows the data within the interquartile range ( 25th and 75th percentiles ) and a solid black line represents the median . Whiskers show maximum and minimum values no further than 1 . 5x IQR ( interquartile range ) . Outlier data are plotted individually . Asterisk indicate statistically significant difference between means analyzed by unpaired , two - tailed and assuming equal variance t - test ( * p < 0 . 05 , * * p < 0 . 01 ) .

O O B-PANEL_START O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B-PANEL_START O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O

Figure 7(A) Primary root length of the different genotypes was measured using the ImageJ program 3 days after 5 mM KNO3 or KCl treatments. Tukey box plot show results from 3 independent biological replicates per experimental condition (n = 8-10 roots each replicate). The box plot shows the data within the interquartile range (25th and 75th percentiles) and a solid black line represents the median. Whiskers show maximum and minimum values no further than 1.5x IQR (interquartile range). Outlier data are plotted individually. Asterisk indicate statistically significant difference between means analyzed by unpaired, two-tailed and assuming equal variance t-test (*p < 0.05, **p < 0.01).(B) Number of initiating and emerging lateral roots for all genotypes were counted using light microscopy 3 days after 5 mM KNO3 or KCl treatments. Tukey box plot show results from 3 independent biological replicates per experimental condition (n = 8-10 roots each replicate). The box plot shows the data within the interquartile range (25th and 75th percentiles) and a solid black line represents the median. Whiskers show maximum and minimum values no further than 1.5x IQR (interquartile range). Outlier data are plotted individually. Asterisk indicate statistically significant difference between means analyzed by unpaired, two-tailed and assuming equal variance t-test (*p < 0.05, **p < 0.01).


Figure 8 ( A ) Confocal microscopy using Zeiss Airyscan microscope and Zeiss - blue3 . 1 software was used to visualize PIN2 in Arabidopsis roots . " e " denotes epidermis and " c " cortex . Scale bar = 20 µm . ( B - D ) Tuckey box plots show PIN2 - GFP fluorescence intensity quantification ( number of roots analyzed per experimental conditions = 8 - 10 ; arbitrary units , a . u . ) at the total cell membrane in roots ( B ) , and from epidermal ( C ) and cortex ( D ) plasma membrane in the different genotypes . The box plot shows the data within the interquartile range ( 25th and 75th percentiles ) and a solid black line represents the median . Whiskers show maximum and minimum values no further than 1 . 5x IQR ( interquartile range ) . Outlier data are plotted individually . Letters denote statistically significant difference between means as determined by one - way ANOVA analysis followed by Tukey HSD post hoc test ( p < 0 . 05 ) .

O O B-PANEL_START O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O

Figure 8(A) Confocal microscopy using Zeiss Airyscan microscope and Zeiss-blue3.1 software was used to visualize PIN2 in Arabidopsis roots. "e" denotes epidermis and "c" cortex. Scale bar = 20 µm. (B-D) Tuckey box plots show PIN2-GFP fluorescence intensity quantification (number of roots analyzed per experimental conditions = 8-10; arbitrary units, a.u.) at the total cell membrane in roots (B), and from epidermal (C) and cortex (D) plasma membrane in the different genotypes. The box plot shows the data within the interquartile range (25th and 75th percentiles) and a solid black line represents the median. Whiskers show maximum and minimum values no further than 1.5x IQR (interquartile range). Outlier data are plotted individually. Letters denote statistically significant difference between means as determined by one-way ANOVA analysis followed by Tukey HSD post hoc test (p < 0.05).