| point,technique,relationship/description/definition/use,factor/label/component/method/mechanism | |
| Migration rate dependency,Electrophoresis,Directly proportional to rate of movement,Charge of the molecule | |
| Migration rate dependency,Electrophoresis,Inversely proportional to rate of movement,Size of the molecule | |
| Migration rate dependency,Electrophoresis,Increased current increases migration rate,Electrical field | |
| Migration rate dependency,Electrophoresis,Increased ionic strength decreases migration rate,Ionic strength of buffer | |
| Migration rate dependency,Electrophoresis,Decreased pH slows migration,pH of buffer | |
| Migration rate dependency,Electrophoresis,Inversely proportional to migration rate,Viscosity of supporting medium | |
| Migration rate dependency,Electrophoresis,High temperature can denature protein and slow migration,System temperature | |
| Analytic electrophoretic procedures,Electrophoresis,Proteins are amphoteric...,Protein electrophoresis | |
| Analytic electrophoretic procedures,Electrophoresis,Similar to protein electrophoresis...,Isoenzyme electrophoresis | |
| Components,Immunoassay,A substance that can elicit an immune response...,Antigen | |
| Components,Immunoassay,An immunoglobulin formed in response to a foreign substance...,Antibody | |
| Immunochemical labels,Immunoassay,"Attached to the antibody, allowing colorimetric quantitation...",Enzyme labels | |
| Immunochemical labels,Immunoassay,"Emit photons when excited, used in fluorescence-based detection...",Fluorescent labels | |
| Immunochemical labels,Immunoassay,Release energy in the form of visible light upon returning to ground state...,Chemiluminescent labels | |
| Immunochemical labels,Immunoassay,"Emit radiation (gamma rays) upon decay, detected for quantitation...",Radioisotope labels | |
| Methodologies,Immunoassay,Enzyme-based,ELISA | |
| Methodologies,Immunoassay,Enzyme-based,EIA/EMIT | |
| Methodologies,Immunoassay,Fluorescence-based,FPIA | |
| Methodologies,Immunoassay,Fluorescence-based,FIA | |
| Methodologies,Immunoassay,Radiation-based,RIA | |
| Methodologies,Immunoassay,Radiation-based,IRMA | |
| Mechanisms of separation,Chromatography,Based on the competition between the sample and the mobile phase for binding sites...,Adsorption chromatography | |
| Mechanisms of separation,Chromatography,Depends on the solubility of the solute in nonpolar or polar solvents...,Partition chromatography | |
| Mechanisms of separation,Chromatography,Involves separation of solutes by their size and charge of the ionic species present...,Ion-exchange chromatography | |
| Chromatographic procedures,Chromatography,Semi-quantitative screening test...,Thin-layer chromatography (TLC) | |
| Chromatographic procedures,Chromatography,Provides quantitative results...,High-performance liquid chromatography (HPLC) |