point,technique,relationship/description/definition/use,factor/label/component/method/mechanism
Migration rate dependency,Electrophoresis,Directly proportional to rate of movement,Charge of the molecule
Migration rate dependency,Electrophoresis,Inversely proportional to rate of movement,Size of the molecule
Migration rate dependency,Electrophoresis,Increased current increases migration rate,Electrical field
Migration rate dependency,Electrophoresis,Increased ionic strength decreases migration rate,Ionic strength of buffer
Migration rate dependency,Electrophoresis,Decreased pH slows migration,pH of buffer
Migration rate dependency,Electrophoresis,Inversely proportional to migration rate,Viscosity of supporting medium
Migration rate dependency,Electrophoresis,High temperature can denature protein and slow migration,System temperature
Analytic electrophoretic procedures,Electrophoresis,Proteins are amphoteric...,Protein electrophoresis
Analytic electrophoretic procedures,Electrophoresis,Similar to protein electrophoresis...,Isoenzyme electrophoresis
Components,Immunoassay,A substance that can elicit an immune response...,Antigen
Components,Immunoassay,An immunoglobulin formed in response to a foreign substance...,Antibody
Immunochemical labels,Immunoassay,"Attached to the antibody, allowing colorimetric quantitation...",Enzyme labels
Immunochemical labels,Immunoassay,"Emit photons when excited, used in fluorescence-based detection...",Fluorescent labels
Immunochemical labels,Immunoassay,Release energy in the form of visible light upon returning to ground state...,Chemiluminescent labels
Immunochemical labels,Immunoassay,"Emit radiation (gamma rays) upon decay, detected for quantitation...",Radioisotope labels
Methodologies,Immunoassay,Enzyme-based,ELISA
Methodologies,Immunoassay,Enzyme-based,EIA/EMIT
Methodologies,Immunoassay,Fluorescence-based,FPIA
Methodologies,Immunoassay,Fluorescence-based,FIA
Methodologies,Immunoassay,Radiation-based,RIA
Methodologies,Immunoassay,Radiation-based,IRMA
Mechanisms of separation,Chromatography,Based on the competition between the sample and the mobile phase for binding sites...,Adsorption chromatography
Mechanisms of separation,Chromatography,Depends on the solubility of the solute in nonpolar or polar solvents...,Partition chromatography
Mechanisms of separation,Chromatography,Involves separation of solutes by their size and charge of the ionic species present...,Ion-exchange chromatography
Chromatographic procedures,Chromatography,Semi-quantitative screening test...,Thin-layer chromatography (TLC)
Chromatographic procedures,Chromatography,Provides quantitative results...,High-performance liquid chromatography (HPLC)