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1
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ุงู„ุงู† ุดุงุก ุงู„ู„ู‡ ุจุฏู†ุง ู†ุญูƒูŠ ููŠู‡ ุงู„ูุญูˆุตุงุช ุงู„ู…ุณุชุฎุฏู…ุฉ
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ู„ู„ูƒุดู ุนู† ุงู„ุฃุญู…ุงุถ ุงู„ุฃู…ูŠู†ูŠุฉ qualitative test of
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amino acid ุจุฏู†ุง ู†ุญูƒูŠ ุนู† ุงู„ูุญูˆุตุงุช ุงู„ู†ูˆุนูŠุฉ ุงู„ู…ุณุชุฎุฏู…ุฉ
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ู„ู„ูƒุดู ุนู† ุงู„ุฃุญู…ุงุถ ุงู„ุฃู…ูŠู†ูŠุฉ qualitative ุชุนู†ูŠ ู†ูˆุนูŠุฉ
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ูŠุนู†ูŠ ู‡ุฐู‡ ุงู„ูุญูˆุตุงุช ุฃูˆ ุงู„ู†ุชูŠุฌุฉ ุงู„ู„ูŠ ู‡ุชุธู‡ุฑ ุนู†ุฏูŠ ู‡ุชูƒูˆู†
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ุนุจุงุฑุฉ ุนู† positive ุฃูˆ negative ูŠูˆุฌุฏ ุฃูˆ ู„ุง ูŠูˆุฌุฏ
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qualitative ู…ุนู†ุงู‡ุง ูุญูˆุตุงุช ู†ูˆุนูŠุฉ ุฃูˆ ุฌูˆุฏุฉ ุชุนุทูŠ ู†ุชูŠุฌุฉ
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positive ุฃูˆ negative ุธู‡ูˆุฑ ู„ูˆู† ุฃูˆ ุนุฏู… ุธู‡ูˆุฑ ู„ูˆู†ุŒ ู„ุง
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ุชุนุทูŠ ุฑู‚ู… ููŠ ุนู†ุฏู†ุง ู†ูˆุนุชุงู† ู…ู† ุงู„ูุญูˆุตุงุช ุงู„ู„ูŠ ู‡ูˆ
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quantitative testQuantitative ู…ุนู†ุงู‡ุง ูƒู…ูŠุฉ ุชุญู„ูŠู„ ูƒู…
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ูŠุนู†ูŠ ุจุฏูŠุทู„ุน ุนู†ุฏูƒ ูƒู…ูŠุฉ ุฃูˆ ู…ู‚ุฏุงุฑ ูŠุนู†ูŠ ู‡ูŠุทู„ุน ุนู†ุฏูƒ ููŠ
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ุงู„ุขุฎุฑ ุนุฏุฏ ุชุฑูƒูŠุฒ ุจุณ ุงู„ู„ูŠ ุงุญู†ุง ู‡ู†ุชุนุฑู ุนู„ูŠู‡ ุงู„ูŠูˆู… ู‡ูˆ
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qualitative test for amino acid ูุญูˆุตุงุช ู†ูˆุนูŠุฉ ู„ู„ูƒุดู
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ุนู† ุงู„ุฃุญู…ุถ ุงู„ุฃู…ูŠู†ูŠุฉููŠ ุงู„ุจุฏุงูŠุฉ ู†ุญูƒูŠ ู…ู‚ุฏู…ุฉ ุนู† ุงู„ุฃุญู…ุงุถ
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ุงู„ุฃู…ูŠู†ูŠุฉ ูˆ ู‡ุฐู‡ ุงู„ุฃุดูŠุงุก ุญูƒูŠู†ุงู‡ุง ููŠ ุงู„ู…ุนู…ู„ ุงู„ู…ุงุถูŠ
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00:01:17,130 --> 00:01:21,850
ุญูƒูŠู†ุง ุฅู† ุงู„ุทุนุงู… ูŠู†ู‚ุณู… ุฅู„ู‰ ุชู„ุงุชุฉ ุฅู„ู‰ carbohydrate ูˆ
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lipid ูˆ protein ุญูƒูŠู†ุง ุงู„ carbohydrate ู‡ูˆ ู…ุตุฏุฑ
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00:01:25,270 --> 00:01:29,470
ุงู„ุทุงู‚ุฉ ุงู„ lipid ู‡ูˆ ู…ุฎุฒู† ุงู„ุทุงู‚ุฉ ูˆ ุงู„ protein ู‡ูˆ
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00:01:29,470 --> 00:01:34,250
ุทุงู‚ุฉ ุงู„ุฎู„ุงูŠุง ู„ุจู†ุงุก ุงู„ุฎู„ุงูŠุง ูˆ ู„ู„ุชุฌุฏ ุญูƒูŠู†ุงู‡ุง ุงู„ู…ุฑุฉ
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00:01:34,250 --> 00:01:39,900
ุงู„ู…ุงุถูŠุฉุจุฑุถู‡ ุญูƒูŠู†ุง ุนู† ุงู„ุฃุญู…ุงุถ ุงู„ุฃู…ูŠู†ูŠุฉ ุฅู†ู‡ ู‡ูŠ ุนุจุงุฑุฉ
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00:01:39,900 --> 00:01:44,180
ุนู† ุงู„ูˆุญุฏุฉ ุงู„ุจู†ุงุฆูŠุฉ ู„ู„ุจุฑูˆุชูŠู† ูˆุญูƒูŠู†ุง ุฅู†ู‡ุง ุจุชุฑุชุจุท ู…ุน
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ุจุนุถ ุจู€protein bond ุญูƒูŠู†ุง ุงู„ุตูŠุบุฉ ุงู„ุนุงู…ุฉ ู„ู„ุฃุญู…ุงุถ
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00:01:47,680 --> 00:01:51,240
ุงู„ุฃู…ูŠู†ูŠุฉ ุนุจุงุฑุฉ ุนู† H ูˆุฅู† ูƒู„ ุญู…ุถ ุฃู…ูŠู† ุจูŠุฎุชู„ู ุนู†
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00:01:51,240 --> 00:01:57,580
ุงู„ุขุฎุฑ ุจุงู„ R ุงู„ side ุงู„ chain ุญูƒูŠู†ุง ูƒู…ุงู† ุงู„ู…ุฑุฉ
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00:01:57,580 --> 00:01:59,780
ุงู„ู…ุงุถูŠุฉ ุฅู† ุงู„ุฃุญู…ุงุถ ุงู„ุฃู…ูŠู†ูŠุฉ
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00:02:03,760 --> 00:02:06,880
ูˆูŠู† ุจุชูŠุฌูŠ ุงู„ุฑุงุจุท ุงู„ู„ูŠ ุจูŠุจุชุฏูŠู‡ุงุŸ ุจูŠู† ู…ุฌู…ูˆุน ูƒุฑุจูˆูƒุณูŠู„
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00:02:06,880 --> 00:02:11,700
ู…ู† ุญู…ุถ ุฃู…ูŠู†ูŠ ูˆู…ุฌู…ูˆุน ุฃู…ูŠู† ู…ู† ุญู…ุถ ุฃู…ูŠู†ูŠ ุขุฎุฑ ูŠุชู… ู†ุฒุน
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00:02:11,700 --> 00:02:15,960
ุฌุฒุก ู…ุงุก ููŠ ู‡ุฐู‡ ุงู„ุนู…ู„ูŠุฉ ูุณู…ูŠู†ุง ุงู„ุชูุงุนู„ุงุช ุชูƒูˆู†
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00:02:15,960 --> 00:02:20,800
ุงู„ุฑุงุจุท ุงู„ู„ูŠ ุจูŠุจุชุฏูŠู‡ุง ุจู€ Condensation Reaction ู„ุฃู†ู‡
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ุจูŠู†ุชุฌ ุนู†ู‡ุง ู…ุงุก ุญูƒูŠู†ุง ุนู„ู‰ ุงู„ุฃุทุฑุงู ุจูŠุธู„ ุนู†ุฏ Free
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00:02:24,500 --> 00:02:28,920
Amino Group ุจู†ุณู…ูŠู‡ุง ุงู„ู€ N Terminus ูˆ Free Carboxyl
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Group ุณู…ูŠู†ุงู‡ุง ุงู„ู€ C Terminusู‡ู†ุง ูƒู…ุงู† ุฅู† ุงู„ุฃุญู…ุงุถ
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ุงู„ุฃู…ู†ูŠุฉ ุชู†ู‚ุณู… ุญุณุจ ุงู„ู…ุตุฏุฑ ุฅู„ู‰ essential ูˆ non
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-essential ุงู„ essential ุงู„ุฌุณู… ุบูŠุฑ ู‚ุงุฏุฑ ุนู„ู‰ ุชุตู†ูŠุนู‡ุง
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00:02:43,320 --> 00:02:47,040
ูŠุฌุจ ุชูˆููŠุฑู‡ุง ููŠ ุงู„ูˆุงุฌุจุงุช ุงู„ non-essential ุงู„ุฌุณู…
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00:02:47,040 --> 00:02:51,420
ู‚ุงุฏุฑ ุนู„ู‰ ุชุตู†ูŠุญู‡ุง ูู…ุด ุถุฑูˆุฑ ุชูƒูˆู† ู…ูˆุฌูˆุฏุฉ ููŠ ุงู„ูˆุงุฌุจุงุช
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00:02:51,420 --> 00:02:58,610
ุงู„ูŠูˆู…ูŠุฉูˆ ู‡ู†ุง ุญูƒูŠู†ุง ุฃู† ุฃุจุณุท ุญู…ุถ ุฃู…ูŠู†ูŠ ู‡ูˆ ุงู„ glycine
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00:02:58,610 --> 00:03:02,590
ู„ูŠุดุŸ ู„ุฃู† ุงู„ side-chain ุฃูˆ ุงู„ R-group ู‡ูŠ ุนุจุงุฑุฉ ุนู†
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00:03:02,590 --> 00:03:06,730
ุทุฑุฏ ู‡ูŠุฏุฑูˆุฌูŠู† ูู‚ุท ูˆ ุญูƒูŠู†ุง ูƒู…ุงู† ุฃู† ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†
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00:03:06,730 --> 00:03:10,990
ุงู„ุดุงุฒ ุฃูˆ ุงู„ูุฑูŠุฏ ุจุงุฎุชู„ู ุนู† ุบูŠุฑู‡ ู…ู† ุงู„ุฃุญู…ุงุถ ุงู„ุฃู…ูŠู†ูŠุฉ
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00:03:10,990 --> 00:03:16,430
ู‡ูˆ ุงู„ุจุฑูˆู„ูŠู†ู„ุฃู†ู‡ ู…ุงุนู†ุฏู‡ูˆุด amino group ุญุฑุฉ ุงู„ู„ูŠ
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00:03:16,430 --> 00:03:19,890
ู…ุชุตู„ุฉ ููŠ ุงู„ู€ central carbon ุณู…ู†ุงู‡ุง primary amino
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00:03:19,890 --> 00:03:24,530
group ููŠ ูƒู„ ุงู„ุฃุญู…ุงุถ ุงู„ุฃู…ูŠู†ูŠุฉ ุชุณู…ู‰ ู…ุฌู…ูˆุนุฉ ุงู„ู€ amino
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00:03:24,530 --> 00:03:30,030
group ู‡ูŠ primary amino group ุฃูˆ free alpha amino
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00:03:30,030 --> 00:03:35,030
group ุฅู„ุง ููŠ ุญุงู„ุฉ ุงู„ุจุฑูˆู„ูŠู† ุชุณู…ู‰ secondary amino
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00:03:35,030 --> 00:03:39,270
group ุฃูˆ amino group ู„ุฃู†ู‡ุง ู…ุชุตู„ุฉ ุจุงู„ู€ central
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00:03:39,270 --> 00:03:47,470
carbon ู…ู† ุฌู‡ุฉ ุจุงู„ู€ sided chainู…ู† ุฌู‡ุฉ ุฃุฎุฑู‰ ุญูƒูŠู†ุง ุนู†
48
00:03:47,470 --> 00:03:51,670
ุงู„ุฃุญู…ุงุถ ุงู„ุฃู…ู†ูŠุฉ ูˆุชู‚ุณูŠู…ู‡ุง ุญุณุจ ุงู„ polarity ุฅู„ู‰ polar
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ูˆ non polar
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00:03:56,470 --> 00:04:00,650
charge ูˆ ุงู„ charge ููŠ ู…ู†ู‡ุง positive charge ูˆ ููŠ
51
00:04:00,650 --> 00:04:04,790
ู…ู†ู‡ุง negative charge ูู‡ุฐู‡ ุงู„ุชู‚ุณูŠู…ุฉ ูƒู„ู‡ุง ุณูˆุงุก polar
52
00:04:04,790 --> 00:04:09,510
ุงูˆ non polar ุชุนุชู…ุฏ ุนู„ู‰ ุงู„ side chain ุจุชุทู„ุน ุนู„ู‰ ุงู„
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00:04:09,510 --> 00:04:13,430
side chain ุญูƒูŠู†ุง ุญูƒูŠ ุงู† ุงุฐุง ููŠ ุจุณ ูƒุฑุจูˆู† ูˆ ู‡ูŠุฏุฑูˆุฌูŠู†
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00:04:13,430 --> 00:04:18,930
ุจุชูƒูˆู† non polar ุงุฐุง ููŠู‡ุง ู…ุฌู…ูˆุนุงุช ู‚ุทุจูŠุฉ ุจุชูƒูˆู† polar
55
00:04:18,930 --> 00:04:22,670
ุจุชุทู„ุน ุงุฐุง ูƒุงู†ุช ู…ุดุญูˆู†ุฉ ุจุชูƒูˆู† charge ูˆ ุงุฐุง ุบูŠุฑ
56
00:04:22,670 --> 00:04:25,390
ู…ุดุญูˆู†ุฉ ุจุชูƒูˆู† uncharged
57
00:04:27,110 --> 00:04:31,130
ุญูƒูŠู†ุง ุนู† ู‡ุฐู‡ ุงู„ุตูˆุฑุฉ ุงู„ู„ูŠ ุจุชู‚ุณู… ู„ู„ุฃุญู…ุงุถ ุงู„ุฃู…ูŠู†ูŠุฉ
58
00:04:31,130 --> 00:04:35,610
ุฅู„ู‰ non-polar ูˆ polar ุงู„ polar ููŠ ู…ู†ู‡ุง uncharged ูˆ
59
00:04:35,610 --> 00:04:39,950
ููŠ ู…ู†ู‡ุง charged ูˆ ููŠ positive ูˆ ููŠ negative ุญูƒูŠู†ุง
60
00:04:39,950 --> 00:04:43,510
ููŠ ุฃุญู…ุงุถ ุฃู…ูŠู†ูŠุฉ ุจู†ุณู…ูŠู‡ุง aromatic amino acid
61
00:04:43,510 --> 00:04:47,070
aromatic ุงู„ู„ูŠ ู‡ูŠ ุจุชุญุชูˆูŠ ููŠ ุงู„ side chain ุนู„ู‰ ุญู„ู‚ุฉ
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00:04:47,070 --> 00:04:50,650
ุจู†ุฒูŠู† ุฅุฐุง ูƒุงู† ููŠ ุงู„ side chain ููŠ ุญู„ู‚ุฉ ุจู†ุณู…ูŠู‡ุง
63
00:04:50,650 --> 00:04:53,290
aromatic amino acid
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00:04:56,910 --> 00:05:00,770
ูˆูŠุฌูŠ ู†ุญูƒูŠ ุนู† ุญุงุฌุฉ ุงุณู…ู‡ุง Ionization Ionization
65
00:05:00,770 --> 00:05:05,110
ู…ุนู†ุงู‡ุง ูƒูŠู ูŠุชุตุฑู ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ูŠ ููŠ ุงู„ุฃูˆุณุงุท
66
00:05:05,110 --> 00:05:10,190
ุงู„ู…ุฎุชู„ูุฉุŒ ููŠ ูˆุณุท ู‚ุงุนุฏูŠุŒ ููŠ ูˆุณุท ุญู…ุถูŠุŒ ููŠ neutralุŒ
67
00:05:10,190 --> 00:05:15,330
ููŠ ุงุชุดุŒ ููŠ ูˆุณุท ุงู„ู…ุชุนุงู†ูŠู‡ู„ุฌุช ู„ูˆ ุฌูŠู†ุง ูˆ ุงุชูุฑุฌ ุนู„ู‰
68
00:05:15,330 --> 00:05:19,210
ุงู„ู…ุนุงุฏู„ุฉ ู‡ุงูŠ ุงู„ู„ูŠ ููˆู‚ ููŠ ุงู„ูˆุณุท ู‡ุฐุง ุญู…ุถ ุฃู…ูŠู†ูŠ ู…ูˆุฌูˆุฏ
69
00:05:19,210 --> 00:05:24,350
ููŠ ูˆุณุท ู…ุชุนุฏุฏ ุงู„ุตูŠุบุฉ ุงู„ุนุงู…ุฉ ู„ุญู…ุถ ุฃู…ูŠู†ูŠ ู…ูˆุฌูˆุฏ ููŠ ูˆุณุท
70
00:05:24,350 --> 00:05:29,790
ู…ุชุนุฏุฏ ุจุชูƒูˆู† ุนุฏุฏ ุงู„ุดุญู†ุงุช ุงู„ู…ูˆุฌุจุฉ ุจุชุณุงูˆูŠ ุนุฏุฏ ุงู„ุดุญู†ุงุช
71
00:05:29,790 --> 00:05:33,690
ุงู„ุณุงู„ู…ุฉ ุงู† ู‡ู†ุง ู…ูƒุชูˆุจ CH2 ู‡ูˆ ุงู„ุตูŠุบุฉ ุงู„ุนุงู…ุฉ ู„ู„ุญู…ุถ
72
00:05:33,690 --> 00:05:38,150
ุงู„ุฃู…ูŠู†ูŠ Central Carbon ูˆ Hydrogen ูˆ ุงู„ R group ู‡ูŠ
73
00:05:38,150 --> 00:05:41,250
ุนุจุงุฑุฉ ุนู† ุฐุฑุฉ ุงู„ู‡ูŠุฏุฑูˆุฌูŠู† ุงู„ู…ุนุชุจุฑูŠู†ู‡ุง ู‡ู†ุงูˆุงู†ู‚ุฏุด
74
00:05:41,250 --> 00:05:45,670
three plus ูˆ COO minus ู‡ูŠ ุงู„ุตูŠุบุฉ ุงู„ุนุงู…ุฉ ู„ู„ุญู…ุฏ
75
00:05:45,670 --> 00:05:49,090
ุงู„ุฃู…ูŠู†ูŠ ููŠ ุงู„ูˆุณุท ุงู„ู…ุชุนุงุฏู„ ุชูƒูˆู† ุงู„ุดุญู†ุงุช ุงู„ู…ูˆุฌุจุฉ
76
00:05:49,090 --> 00:05:54,830
ุจุชุณุงูˆูŠ ุงู„ุดุญู†ุงุช ุงู„ุณุงู„ู…ุฉุงู„ุฌู‡ุงุฏ ุงู„ู€ net charge ุจุชูƒูˆู†
77
00:05:54,830 --> 00:05:59,330
ุฒูŠู‡ ู„ูˆ ุฌูŠุช ุฃุฎุฏุช ู‡ุฐุง ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ูŠ ุญุงุทูŠุชู‡ ููŠ ูˆุณุท
78
00:05:59,330 --> 00:06:07,010
ุญู…ุถูŠ ูˆุณุท ุญู…ุถูŠ ูŠุนู†ูŠ ุฌู„ุงู„ุฉ ุงู„ู€ pH ูŠุนู†ูŠ ุฃู†ู‡ ูƒุงู† ููŠู‡
79
00:06:07,010 --> 00:06:11,130
ุฒูŠุงุฏุฉ ููŠ ุฃูŠูˆู†ุงุช ุงู„ู‡ูŠุฏุฑูˆุฌูŠู† ูŠุนู†ูŠ ู‡ุฐุง ุงู„ูˆุณุท ู…ู„ูŠุก ุจู€
80
00:06:11,130 --> 00:06:16,620
H plus ุฒูŠุงุฏุฉูุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ู‰ ุฏุฎู„ุชู‡ ู…ู† ุงู„ู…ุชุนุฏู„ ูˆุญุทูŠุชู‡
81
00:06:16,620 --> 00:06:20,120
ููŠ ูˆุณุท ุงู„ุญู…ุถูŠ ูˆุณุท ุงู„ุญู…ุถูŠ ููŠู‡ ุฒูŠุงุฏุฉ ููŠ ุฃูŠูˆู†ุงุช
82
00:06:20,120 --> 00:06:24,400
ุงู„ู‡ูŠุฏุฑูˆุฌูŠู† ุงู„ุฌุงุชู…ูŠู† ุจุฏู‡ ูŠุญุชุงุฌ ุงู„ู‡ูŠุฏุฑูˆุฌูŠู† ู‡ูŠุงุฎุฏ ู…ู†
83
00:06:24,400 --> 00:06:28,920
ุงู„ูˆุณุท COO- ู…ุญุชุงุฌ ุงู„ู‡ูŠุฏุฑูˆุฌูŠู† ูุจุชุณุชุญุจ ู…ู† ุงู„ูˆุณุท
84
00:06:28,920 --> 00:06:37,990
ู‡ูŠุฏุฑูˆุฌูŠู† ูˆ ุจุชุชุดุจุน ุจ CRCOOHูุจุชุตูŠุฑ CH2 COOH NH3 Plus
85
00:06:37,990 --> 00:06:44,990
ุจุตูŠุฑ ุงู„ุดูƒู„ ุงู„ุนุงู… ู„ู‡ CH2 COOH NH3 Plus ูุฃุฎุฐ ุฐุฑุฉ
86
00:06:44,990 --> 00:06:49,960
ู‡ูŠุฏุฑูˆุฌูŠู†ูŠ ุตุงุฑุช ุงู„ู†ุช ุชุดุงุฑุฌ ุนู„ูŠู‡ positive chargeุงู„ู„ูŠ
87
00:06:49,960 --> 00:06:53,040
ู…ูˆุฌูˆุฏุฉ ููŠ ุงู„ู…ุนุงุฏู„ุฉ ุนู„ู‰ ุงู„ูŠุณุงุฑ ู‡ุฐุง ุงู„ู„ูŠ ู‡ูˆ ุงู„ุตูŠุบุฉ
88
00:06:53,040 --> 00:06:59,480
ุงู„ุนุงู…ุฉ ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ูŠ ููŠ ูˆุณุท ุญู…ุถูŠ CH2COHNH3Plus
89
00:06:59,480 --> 00:07:03,580
ุฅู†ู‡ ุงู„ูƒุฑุจูˆูƒุณูŠู„ ุฃุฎุฏุช ุฃูŠูˆู† ู‡ูŠุฏุฑูˆุฌูŠู† ุฃูˆ ุจุฑูˆุชูˆู†
90
00:07:03,580 --> 00:07:08,720
ู‡ูŠุฏุฑูˆุฌูŠู† ู…ู† ุงู„ูˆุณุท ุงู„ุญู…ุถูŠ ุงู„ู„ูŠ ู‡ูŠ ููŠู‡ ูุตุงุฑุช COH ู†ุช
91
00:07:08,720 --> 00:07:13,960
ุชุดุงุฑุฌ ููŠ ุงู„ูˆุณุท ุงู„ุญู…ุถูŠ ุตุงุฑุชPositive charge ุทุจุนุง ู„ูˆ
92
00:07:13,960 --> 00:07:18,060
ุฃุฎุฏุชู‡ุง ุงู„ูˆุณุท ู…ู† ุงู„ูˆุณุท ุงู„ู…ุชุนุฏุฏ ูˆุญุทุชู‡ ููŠ ูˆุณุท ู‚ุงุนุฏูŠ
93
00:07:18,060 --> 00:07:25,280
ุงู„ูˆุณุท ุงู„ู‚ุงุนุฏูŠ ู…ู„ูŠุก ุจู€ OH- ูˆุณุท ุจูŠูƒูˆู† ู…ู„ูŠุก ุจู€ OH- OH
94
00:07:25,280 --> 00:07:30,140
- ู‡ูŠ ุชุญุชุงุฌ ุฅู„ู‰ ุฐุฑุฉ ู‡ูŠุฏุฑูˆุฌูŠู† ุนุดุงู† ุชุชูƒูˆู† ุฌุฒุฆู†ุง
95
00:07:30,140 --> 00:07:35,920
ูุจุชุฑูˆุญ ุนู„ู‰ ุฃูŠ ุฐุฑ ุฌุฒูŠุฉ ุจุชู†ุฒุน ู…ู†ู‡ ู‡ูŠุฏุฑูˆุฌูŠู† ุนุดุงู†
96
00:07:35,920 --> 00:07:40,320
ุชุชูƒูˆู†ู†ุง ู…ู† ุนู†ุฏู†ุง ุจู†ู‚ุฏุฑ ู†ู†ุฒุน ู…ู†ู‡ ู‡ูŠุฏุฑูˆุฌูŠู† ู„ุฃู† ุงุชุด 3
97
00:07:40,320 --> 00:07:46,960
plusุชุงุฎุฏ ู…ู†ู‡ ู‡ูŠุฏุฑูˆุฌูŠู† ูุจุตูŠุฑ NH2 COO minus ู‡ุชุชุบูŠุฑุŸ
98
00:07:46,960 --> 00:07:52,300
ู„ุฃ ู…ุด ู‡ุชุชุบูŠุฑ ูุงู„ูˆุณุท ุงู„ู…ุชุนุฏู„ CH2 NH3 plus COO minus
99
00:07:52,300 --> 00:07:57,360
ู„ู…ุง ูŠุฑูˆุญ ุนู„ู‰ ุงู„ู‚ุงุนุฏุฉ ุงู„ู„ูŠ ู‡ูŠ ุนู„ู‰ ุงู„ูŠู…ูŠู† ุจูŠุตูŠุฑ NH2
100
00:07:57,360 --> 00:08:02,860
ูˆ COO minus ุงุชุบูŠุฑุช ู…ู† ุงู„ู€ NH3 ู†ุฒุนู†ุง ู…ู†ู‡ุง ู‡ูŠุฏุฑูˆุฌูŠู†
101
00:08:02,860 --> 00:08:07,600
ูˆ ุตุงุฑุช NH2ุงู„ู€ net charge ุฅูŠู‡ ู„ูˆ ุจูŠูƒูˆู† minus ูููŠ
102
00:08:07,600 --> 00:08:11,920
ุญุงู„ุฉ ุงู„ูˆุณุท ุงู„ุญู…ุถูŠ .. ููŠ ุญุงู„ุฉ ูˆุณุท ู…ุชุนุงุฏู„ ุงู„ net
103
00:08:11,920 --> 00:08:16,360
charge ุจุชูƒูˆู† zero ูุญุงู„ุฉ ูˆุณุท ุญู…ุถูŠ ุจูŠูƒูˆู† ุงู„ net
104
00:08:16,360 --> 00:08:20,100
charge positive ููŠ ุญุงู„ุฉ ุงู„ูˆุณุท ุงู„ู‚ุงุนุฏูŠ ุงู„ net
105
00:08:20,100 --> 00:08:24,700
charge negativeู‡ู„ุฌุฏุŸ ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ูŠ ุงู„ู„ูŠ ุจุชูƒูˆู†
106
00:08:24,700 --> 00:08:29,120
ู…ุชุณุงูˆูŠ ุนู„ูŠู‡ ุงู„ุดุญู†ุงุช ุงู„ู…ูˆุฌุจุฉ ูˆ ุงู„ุดุญู†ุงุช ุงู„ุณุงู„ุจุฉ ูˆ ุงู„
107
00:08:29,120 --> 00:08:32,960
net charge ุจุชูƒูˆู† ุจุชุณุงูˆูŠ zero ุจุณู…ูŠู‡ Zwitterion
108
00:08:32,960 --> 00:08:37,580
Zwitterion ู‡ูˆ ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ูŠ ุงู„ู„ูŠ ุชุชุณุงูˆูŠ ุนู„ูŠู‡
109
00:08:37,580 --> 00:08:42,180
ุงู„ุดุญู†ุฉ ุงู„ู…ูˆุฌุจุฉ ูˆ ุงู„ุดุญู†ุฉ ุงู„ุณุงู„ุจุฉ ูŠุนู†ูŠ ุงู„ุดุญู†ุฉ ุงู„ูƒู„ูŠุฉ
110
00:08:42,180 --> 00:08:46,280
ุชูƒูˆู† ุตูุฑ ูˆูŠู† ู…ูˆุฌูˆุฏ ุงู„ ZwitterionุŸ ููŠ ุงู„ูˆุณุท
111
00:08:46,280 --> 00:08:50,650
ุงู„ู…ุชุนุงุฏู„ ูƒุงู† ู…ูˆุฌูˆุฏุจุงู„ูˆุณุท ุงู„ู…ุชุนุฏู„ ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ูŠ
112
00:08:50,650 --> 00:08:54,790
ุจูŠูƒูˆู† ุงู„ุดุญู† ุงู„ู…ูˆุฌุจุฉ ุจุชุณุงูˆูŠ ุงู„ุดุญู† ุงู„ุณุงู„ูŠ ูŠุนู†ูŠ ุงู„ุดุญู†
113
00:08:54,790 --> 00:08:59,950
ุงู„ูƒู„ูŠุฉ ุจุชุณุงูˆูŠ ุณูุฑ ูˆู‡ุฐุง ุจุณู…ูŠู‡ Zwitterion Zwitterion
114
00:08:59,950 --> 00:09:03,870
ู‡ูˆ ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ูŠ ุงู„ุฐูŠ ุชุชุณุงูˆูŠ ุนู„ูŠู‡ ุงู„ุดุญู† ุงู„ู…ูˆุฌุจุฉ
115
00:09:03,870 --> 00:09:10,520
ูˆุงู„ุดุญู† ุงู„ุณุงู„ูŠ ูˆู†ุฏุช ุดู‡ุฑ Zeroุนู†ุฏ ุงู„ูˆุณุท ุงู„ู…ุชุนุงุฏู„ ู‡ุฐุงุŒ
116
00:09:10,520 --> 00:09:13,240
ุนู†ุฏ ุชูƒูˆู† ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ูŠ ุงู„ู„ูŠ ุจุชูƒูˆู† ุดุญู†ุงุช ุงู„ู…ูˆุฌุจุฉ
117
00:09:13,240 --> 00:09:16,340
ุงู„ุณุงู„ุจุฉ ุจุชุณุงูˆูŠ ุงู„ู…ูˆุฌุจุฉุŒ ููŠ ุญุงุฌุฉ ุจู†ุชุณู…ูŠู‡ุง
118
00:09:16,340 --> 00:09:21,100
isoelectric point ุฃูˆ ุงู„ู€ PR ุงู„ู€ PI ุฃูˆ ุงู„ู€
119
00:09:21,100 --> 00:09:26,540
isoelectric point ู‡ูŠ ู‚ูŠู…ุฉ BH ุจุชุชุณุงูˆูŠ ุนู†ุฏ ุงู„ุดุญู†
120
00:09:26,540 --> 00:09:32,320
ุงู„ู…ูˆุฌุจุฉ ูˆุงู„ุณุงู„ุจุฉ ุนู„ู‰ ุณุทุญ ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ูŠูˆุจุงู„ุชุงู„ูŠุŒ
121
00:09:32,320 --> 00:09:38,620
ุดุญู†ุฉ ุงู„ูƒู„ูŠุฉ ุตูุฑ ู‚ูŠู…ุฉ BH ุชุชุณุงูˆู‰ ุนู†ุฏู‡ุง ุดุญู†ุฉ ุงู„ู…ูˆุฌุจุฉ
122
00:09:38,620 --> 00:09:43,760
ูˆุงู„ุณุงู„ุจุฉ ุนู„ู‰ ุณุทุญ ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู† ูˆุจุงู„ุชุงู„ูŠุŒ ุดุญู†ุฉ
123
00:09:43,760 --> 00:09:47,640
ุงู„ูƒู„ูŠุฉ ุจุชุณุงูˆูŠ ุตูุฑ ู‡ุงูŠ ุงู„ู€ Isoelectric pointุŒ
124
00:09:47,640 --> 00:09:53,260
ุงู„ุฅูุชุตุงุฑ ุจู€I ุชู…ุงู…ุŒ ููŠ ุญุงุฌุฉ ุจู†ุณู…ูŠู‡ุง protonated
125
00:09:53,260 --> 00:09:57,740
ู…ุนู†ุงู‡ุง ุฅู†ู‡ ุงูƒุชุณุงุจ ุจุฑูˆุชูˆู† ุฃูˆ H plus ููŠ ุญุงุฌุฉ ุจู†ุณู…ูŠู‡ุง
126
00:09:57,740 --> 00:10:02,780
Deprotonated ูŠุนู†ูŠ ูู‚ุฏ ุจุฑูˆุชูˆู†ุงู„ู„ูŠ ุจูŠุงุฎุฏ H plus ูŠุนู†ูŠ
127
00:10:02,780 --> 00:10:07,800
ููŠ ุงู„ูˆุณุท ุงู„ุญู…ุถ ุฒูŠ COO minus ุงูƒุชุณุจุช H ูู‡ุฐู‡ gain of
128
00:10:07,800 --> 00:10:12,800
ุงู„ุจุฑูˆุชูˆู† ูŠุนู†ูŠ ุตุงุฑู„ู‡ุง protonated Deprotonated ูŠุนู†ูŠ
129
00:10:12,800 --> 00:10:16,760
loss of ุงู„ุจุฑูˆุชูˆู† ุฒูŠ ุงู„ู€ NH3 plus ููŠ ุงู„ูˆุณุท ุงู„ู‚ุงุนุฏุฉ
130
00:10:16,760 --> 00:10:22,060
ู„ู…ุง ุญุถูŠุชู‡ุง ุตุงุฑุช NH2 ู†ุฒุนุช ู…ู†ู‡ุง H ูุจุณู…ูŠู‡ุง .. ุจู‚ูˆู„
131
00:10:22,060 --> 00:10:26,600
ุนู†ู‡ุง ู†ุตุงุฑู„ู‡ุง Deprotonated ูู‚ุฏุช ุงู„ุจุฑูˆุชูˆู†
132
00:10:30,550 --> 00:10:34,490
ู‡ู†ุง ุจูŠุญูƒูŠู„ูŠ ุฃู† ุงู„ู€ Isoelectric point ุฃูˆ ุงู„ู€ PI ู‡ูŠ
133
00:10:34,490 --> 00:10:39,090
ู‚ูŠู…ุฉ BH ุงู„ู„ูŠ ุจูŠูƒูˆู† ููŠู‡ุง ุงู„ุงู†ูŠูˆู†ูƒ ุจุชุณุงูˆูŠ ุงู„ูƒุงุชูŠูˆู†ูƒ
134
00:10:39,090 --> 00:10:45,350
ุงู„ู†ุช ุชุดุงุฑุฌ ุจุชูƒูˆู† Zero ูƒู„ ุญู…ุถ ุฃู…ูŠู†ูŠ ู„ู‡ PI ุฃูˆ
135
00:10:45,350 --> 00:10:49,490
Isoelectric point ุจุชุฎุชู„ู ุนู† ุบูŠุฑู‡ ุญุณุจ ุดูˆุŸ ุญุณุจ ุงู„
136
00:10:49,490 --> 00:10:52,830
side chain ูู‡ูˆ ุญุงูƒูŠ ุฃู† ูƒู„ ุญู…ุถ ุฃู…ูŠู†ูŠ ุจูŠู…ูŠุฒู‡ ุนู† ุบูŠุฑู‡
137
00:10:52,830 --> 00:10:54,110
side chain
138
00:10:56,690 --> 00:11:01,790
ู†ุญูƒูŠ ููŠ ุงู„ุฌุฒุก ุงู„ุนู…ู„ูŠ ู„ู„ู…ุนู…ู„ ุงู„ุฃูˆู„ ุฌุฒุก ุงู„ุนู…ู„ูŠ ุจูŠุญูƒูŠ
139
00:11:01,790 --> 00:11:06,930
ุนู† qualitative test for amino acid ุจุญูˆุตุงุช ุฌูˆุฏุฉ
140
00:11:06,930 --> 00:11:10,710
ู„ุงู„ูƒุดู ุนู† ุงู„ุฃุญู…ุงุถ ุงู„ุฃู…ูŠู†ูŠุฉ ุงู„ู†ุชูŠุฌุฉ ุนู†ุฏู†ุง ู‡ุชูƒูˆู†
141
00:11:10,710 --> 00:11:16,330
positive ุฃูˆ negativeยซุธู‡ูˆุฑ ู„ูˆู†ยป ุฃูˆ ยซุนุฏู… ุธู‡ูˆุฑ ู„ูˆู†ยป
142
00:11:16,330 --> 00:11:19,450
ููŠ ุนู†ุฏู†ุง number of tests to detect the presence of
143
00:11:19,450 --> 00:11:23,210
amino acid ู‡ุฐู‡ ุงู„ูุญูˆุตุงุช ุงู„ูƒุงุดู ุนู† ุงู„ุฃุญู…ุงู† ุงู„ุฃู…ูŠู†ูŠุฉ
144
00:11:23,210 --> 00:11:26,830
largely depend on the nature of sided chain ุนู„ุดุงู†
145
00:11:26,830 --> 00:11:29,770
ุจูŠุนุชู…ุฏูˆุง ุนู„ู‰ ุงู„ู€sided chain ู„ุฃู†ู‡ ุญูƒูŠ ุฃู† ุงู„ุจูŠู…ูŠุฒ
146
00:11:29,770 --> 00:11:34,090
ุญู…ุถ ุฃู…ูŠู† ุนู† ุงู„ุขุฎุฑ ู‡ูˆ ุงู„ู€sided chain ุงู„ุฌุฏ ุงุญู†ุง
147
00:11:34,090 --> 00:11:38,210
ุงู„ูุญูˆุตุงุช ุงู„ู„ูŠ ู‡ู†ุดุชุบู„ู‡ุง ุจูŠุทู„ุน ู…ุนู†ุง ู„ูˆู† ูŠุชุบูŠุฑ ุฃูˆ
148
00:11:38,210 --> 00:11:44,060
ู…ุงูŠุชุบูŠุฑู‡ุฐุง ุงู„ู„ูˆู† ู„ู‡ ุดุฏุฉ intensity of color ุดุฏุฉ
149
00:11:44,060 --> 00:11:47,940
ุงู„ู„ูˆู† ู‡ูŠ ุจุชูƒูˆู† ุชุฎุชู„ู ูŠุนู†ูŠ ู…ู…ูƒู† ู‡ูˆ ูŠุชู„ูˆู† ุจุณ ุนู„ู‰
150
00:11:47,940 --> 00:11:52,580
ุฏุฑุฌุงุช ู…ู† ุญุงุฌุฉ ู„ุชุงู†ูŠุฉ ุชูƒูˆู† ุฏุฑุฌุฉ ุจุชุฎุชู„ู ุดุฏุฉ ุงู„ู„ูˆู† ู‡ูŠ
151
00:11:52,580 --> 00:11:56,860
ุนู„ุดุงู† ุจุชุนุชู…ุฏ ุจุชุนุชู…ุฏ ุนู„ู‰ ุฃูˆู„ ุดูŠ number of reacting
152
00:11:56,860 --> 00:12:00,900
unit number of reacting unit ุงู„ู„ูŠ ู‡ูŠ ุงู„ reacting
153
00:12:00,900 --> 00:12:05,040
unit ู‡ูŠ ุนุจุงุฑุฉ ุนู† ุงู„ู…ุงุฏุฉ ุงู„ูุนุงู„ุฉู‚ุฏุงุด ุนุฏุฏ ุงู„ู…ุงุฏุฉ
154
00:12:05,040 --> 00:12:08,780
ุงู„ูุนุงู„ุฉ ุงู„ู…ูˆุฌูˆุฏุฉุŸ ุฅูŠุด ู‡ูŠ ุงู„ู…ุงุฏุฉ ุงู„ูุนุงู„ุฉุŸ ุฏูŠ ุงู„ุงุดูŠ
155
00:12:08,780 --> 00:12:12,940
ุงู„ู„ูŠ ุจุฏูˆุฑ ุนู„ูŠู‡ุง ุงู„ูุญุต ุฃู†ุง ุจุดุชุบู„ ูุญุต ู…ุซู„ุง ุจูŠุฏูˆุฑ ุนู„ู‰
156
00:12:12,940 --> 00:12:15,980
peptide bond ุฅุฐุง ู…ูˆุฌูˆุฏุฉ ุงู„ peptide bondุŒ ูŠุนุทูŠู†ูŠ
157
00:12:15,980 --> 00:12:19,900
ู„ูˆู† ุฅุฐุง ู…ุด ู…ูˆุฌูˆุฏุฉุŒ ู…ุด ู‡ูŠุชุบูŠุฑ ุงู„ู„ูˆู† ุทุจ ุงู„ peptide
158
00:12:19,900 --> 00:12:23,160
bond ู‡ุฐูŠ ู…ู…ูƒู† ุชูƒูˆู† ุจุณ ุฑุงุจุทุชูŠู†ุŒ ูˆูŠุนุทูŠู†ูŠ ู„ูˆู† ู…ู…ูƒู†
159
00:12:23,160 --> 00:12:26,400
ุชูƒูˆู† ุชู„ุงุชุฉ ูˆูŠุนุทูŠู†ูŠ ู„ูˆู† ุจุณ ู‡ู„ ุงู„ู„ูˆู† ู‡ูŠูƒูˆู† ู†ูุณู‡ ุฅุฐุง
160
00:12:26,400 --> 00:12:30,080
ูƒุงู†ูˆุง ุชู†ุชูŠู† ูˆุฅุฐุง ูƒุงู†ูˆุง ุชู„ุงุชุฉุŸ ุดุฏุฉ ุงู„ู„ูˆู† ู‡ุชุฎุชู„ู
161
00:12:30,080 --> 00:12:34,810
ุจู†ุงุก ุนู„ู‰ number of reacting unitุงู„ุญุงุฌุฉ ุงู„ุชุงู†ูŠุฉ
162
00:12:34,810 --> 00:12:37,890
ุงู„ู„ูŠ ุจุชุนุชู…ุฏ ุนู„ูŠู‡ุง ุดุฏุฉ ุงู„ู„ูˆู† ู‡ูŠ ุงู„ู€ accessibility
163
00:12:37,890 --> 00:12:42,050
to reaction ู‡ู„ ู‡ุฐู‡ ุงู„ู…ุงุฏุฉ ุงู„ูุนุงู„ุฉ ุงู„ู€ acting unit
164
00:12:42,050 --> 00:12:46,350
ู‚ุฑูŠุจุฉ ู…ู† ุงู„ุชูุงุนู„ุŒ ู‡ู„ ู‡ูŠ ุญุฑุฉุŒ freeุŒ ุจุชู‚ุฏุฑ ุชูŠุฌูŠ
165
00:12:46,350 --> 00:12:48,990
ุชุฑุชุจุทุŒ ุชุดุชุบู„ ู…ุน ุงู„ุชูุงุนู„ุŒ ู…ุน ุงู„ู€ agent ุงู„ู„ูŠ ุฃู†ุง
166
00:12:48,990 --> 00:12:52,910
ุญุทูŠุชู‡ุŒ ุชุดุชุบู„ ูˆ ุชุชุบูŠุฑ ุงู„ู„ูˆู†ุŒ ูˆู„ุง ุฅู†ู‡ุง ู…ุดุบูˆู„ุฉุŒ ู…ุด
167
00:12:52,910 --> 00:12:56,930
ู‡ุชู‚ุฏุฑ ููŠ ุญูˆุงุฌุฒุŒ ู…ุด ู‡ุชู‚ุฏุฑ ุชูŠุฌูŠ ุชุฑุชุจุทู‡ูŠ ุจุชุณู…ูŠู‡ุง
168
00:12:56,930 --> 00:13:00,810
accessibility to reaction ุฅุฐุง ุชุดุฏุช ุงู„ู„ูˆู† ุชุนุชู…ุฏ ุนู„ู‰
169
00:13:00,810 --> 00:13:04,450
ุญุงุดุชูŠู† ุนู„ู‰ ุงู„ number of reacting unit ูˆุนู„ู‰
170
00:13:04,450 --> 00:13:10,470
accessibility to reaction ุฅูŠุด
171
00:13:10,470 --> 00:13:13,570
ู‡ูŠ ุงู„ูุญุตุงุช ุงู„ู„ูŠ ู‡ู†ุณุชุฎุฏู…ู‡ุง ู„ู„ูƒุดู ุนู† ุงู„ุฃุญู…ุงุถ
172
00:13:13,570 --> 00:13:19,070
ุงู„ุฃู…ูŠู†ูŠุฉุŸ ู‡ู†ุง ูุญุต ุงุณู…ู‡ ninhydrin test for 3-alpha
173
00:13:19,070 --> 00:13:24,160
amino groupุนู„ู‰ Free Alpha Amino Group ุฅุฐุง ู…ูˆุฌูˆุฏุฉ
174
00:13:24,160 --> 00:13:27,280
ุจูŠุชุบูŠุฑ ุงู„ู„ูˆู† ูˆุจูŠุนุทู‰ positive ุฅุฐุง ู…ุด ู…ูˆุฌูˆุฏุฉ ู…ุด
175
00:13:27,280 --> 00:13:30,880
ู‡ูŠุชุบูŠุฑ ุงู„ู„ูˆู† ูุงู„ู€ Free Alpha Amino Group ู‡ุงูŠ
176
00:13:30,880 --> 00:13:35,000
ุจุณู…ูŠู‡ุง ุงู„ู€ Reacting Unit ูุญุต ุงู„ุชุงู†ูŠ ุงุณู…ู‡ Bio-Red
177
00:13:35,000 --> 00:13:39,240
Test ุจุชุฏูˆุฑ ุนู„ู‰ ุงู„-peptide bond ู„ุฅู†ู‡ ูุญุต ุงู„
178
00:13:39,240 --> 00:13:45,040
-xanthoproteic testZanto Proteic Test for Aromatic
179
00:13:45,040 --> 00:13:51,240
Amino Acid ู„ุฃ
180
00:13:51,240 --> 00:13:55,300
ุงู„ุฃุญู…ุงุถ ุงู„ุฃู…ู†ูŠุฉ ุงู„ู„ูŠ ุชุญุชูˆูŠ ุนู„ู‰ ุญู„ู‚ุฉ ุจู†ุฒูŠู† ู†ุดุทุฉ
181
00:13:55,300 --> 00:14:00,720
ู†ุถูŠู ู‡ู†ุง Activated Aromatic Amino Acidู‡ู†ุง ูุญุต ุงุณู…ู‡
182
00:14:00,720 --> 00:14:05,240
hopkincol ุฃูˆ glycosilic acid reaction ุงู„ู„ูŠ ู‡ูˆ ุงุณู…ู‡
183
00:14:05,240 --> 00:14:09,580
hopkincolุŒ glycosilic acid reaction ุจูŠุฏูˆุฑ ุนู„ู‰ ุงู„ู€
184
00:14:09,580 --> 00:14:13,920
Indole group ุงู„ู€ melons test for amino acid
185
00:14:13,920 --> 00:14:18,800
containing hydroxylููŠู†ุงู„ ุฌุฑูˆุจ ู„ู„ููŠู†ูˆู„ ุฌุฑูˆุจ ุงู„ุฃู…ูŠู†ูˆ
186
00:14:18,800 --> 00:14:23,100
ุฃุณุฏ ุงู„ู„ูŠ ุจูŠุญุชูˆูŠ ุนู„ู‰ ููŠู†ูˆู„ ุฌุฑูˆุจ ุณุงูƒูŠุฌูˆุดูŠ test for
187
00:14:23,100 --> 00:14:28,160
ู„ูŠุฌูˆุงู†ูŠุฏูŠุงู… ุฌุฑูˆุจ ู„ู„ูŠุฌูˆุงู†ูŠุฏูŠุงู… ุฌุฑูˆุจ ู„ูŠุฏ ุณุงู„ูุงูŠุช
188
00:14:28,160 --> 00:14:33,080
test ุจูŠุฏูˆุฑ ุนู„ู‰ ุงู„ self-hydraile group ู„ู„ุญู…ุถ ุงู„ุฃู…ูŠู†
189
00:14:33,080 --> 00:14:36,240
ุงู„ู„ูŠ ูŠุญุชูˆูŠ ุนู„ู‰ self-hydraile group ู‡ูˆ ุงู„ู„ูŠ ู‡ูŠุนุทูŠู†ูŠ
190
00:14:36,240 --> 00:14:39,360
positive ุบูŠุฑ ู‡ูŠูƒ ู…ุด ู‡ูŠุนุทูŠู†ูŠ positive
191
00:14:42,170 --> 00:14:46,290
ู†ุฌูŠ ู†ุญูƒูŠ ุนู† ุฃูˆู„ ูุญุตุŒ ุฃูˆู„ ูุญุต ู‡ูˆ ุงู„ู€ Nenhedrine ุงู„ู€
192
00:14:46,290 --> 00:14:49,010
Nenhedrine ุฅูŠุด ุงู„ objective ู„ู„ูุญุตุŒ ุฅูŠุด ุงู„ู‡ุฏู ู…ู†
193
00:14:49,010 --> 00:14:52,730
ุงู„ูุญุตุŸ to detect the presence of free alpha amino
194
00:14:52,730 --> 00:14:56,950
groupุŒ ุจูŠุฏูˆุฑ ุนู„ู‰ ุงู„ู€ free alpha amino group ุฅุฐุง
195
00:14:56,950 --> 00:15:00,970
ู…ูˆุฌูˆุฏ free alpha amino groupุŒ ู‡ูŠุชุบูŠุฑ ุงู„ู„ูˆู†ุŒ
196
00:15:00,970 --> 00:15:04,330
ุจูŠุนุทูŠู†ูŠ ู„ูˆู† ุจุงุฑุฏุŒ ููŠ ุญุงู„ุงุช ุงู„ู„ูŠ ู…ูˆุฌูˆุฏุฉ ููŠ free
197
00:15:04,330 --> 00:15:07,930
alpha amino groupู…ูŠู† ุนู†ุฏู‡ free alpha amino groupุŸ
198
00:15:07,930 --> 00:15:11,390
ุฅุฐุง ุฐุงูƒุฑูŠู† ุงู„ุญู…ุถ ุงู„ุฃู…ูŠู†ูŠุŒ ุฃูŠุด ุงู„ุตูŠุบุฉ ุงู„ุนุงู…ุฉ ู„ู‡ุŸ
199
00:15:11,390 --> 00:15:14,750
ุชูƒูˆู† ู…ู† center carbon ูˆู…ู† ุงู„ู‡ูŠุฏุฑูˆุฌูŠู†ุฉ ูˆู…ู† ุงู„ side
200
00:15:14,750 --> 00:15:18,530
chain ูˆู…ู† carboxyl group ูˆู…ู† amino group amino
201
00:15:18,530 --> 00:15:23,350
group ู‡ุงูŠ ุณู…ูŠู†ุงู‡ุง primary amino group ุฃูˆ alpha
202
00:15:23,350 --> 00:15:27,530
amino group ู…ุนุงุฏุฉ ููŠ ุญุงู„ุฉ ุงู„ุจุฑูˆู„ูŠู† ุณู…ูŠู†ุงู‡ุง amino
203
00:15:27,530 --> 00:15:32,840
groupูŠุนู†ูŠ ุงู„ุฃุญู…ุงุถ ุงู„ุฃู…ูŠู†ูŠุฉ ูƒู„ู‡ุง ุชุญุชูˆูŠ ุนู„ู‰ free
204
00:15:32,840 --> 00:15:37,700
alpha amino group ู…ุง ุนุฏุง ู„ุจุฑูˆู„ูŠู† ุนู†ุฏู‡ amino group
205
00:15:37,700 --> 00:15:40,760
ูŠุนู†ูŠ ู…ูŠู† ู‡ูŠุนุทูŠู†ูŠ positive ู…ุน ู‡ุฐุง ุงู„ูุญุต ู„ูˆ ุฃู†ุง
206
00:15:40,760 --> 00:15:44,440
ุงุดุชุบู„ุช ู…ุฌู…ูˆุนุฉ ุฃุญู…ุงุถ ุฃู…ูŠู†ูŠุฉ ุนู„ู‰ ู‡ุฐุง ุงู„ูุญุตุŸ ูƒู„ู‡ู…
207
00:15:44,440 --> 00:15:47,540
ู‡ูŠุนุทูˆู†ูŠ positive ู„ุฃู†ู‡ู… ูƒู„ู‡ู… ุจูŠุญุชูˆูŠ ุนู„ู‰ free alpha
208
00:15:47,540 --> 00:15:53,040
amino group ุชู…ุงู…ุŸุทูŠุจุŒ ุฅุฐุง ุงู„ objective to detect
209
00:15:53,040 --> 00:15:55,520
the presence of free alpha amino group ู‡ู„ ู…ูˆุฌูˆุฏ
210
00:15:55,520 --> 00:15:58,420
free alpha amino group ุฃูˆ ู„ุงุŸ ุฅุฐุง ุชุบูŠุฑ ุงู„ู„ุบุฉ ูŠุนู†ูŠ
211
00:15:58,420 --> 00:16:01,560
ู…ูˆุฌูˆุฏุŒ ุฅุฐุง ู…ุง ุชุบูŠุฑุด ูŠุนู†ูŠ ู…ุด ู…ูˆุฌูˆุฏ ุทูŠุจ ุฅูŠุด ุงู„
212
00:16:01,560 --> 00:16:05,620
principleุŸ ู…ุจุชุฏุฃ ุงู„ุนู…ู„ ู„ู„ูุญุตุŸ ูƒูŠู ูŠุดุชุบู„ ุงู„ูุญุตุŸ ุงู„
213
00:16:05,620 --> 00:16:08,520
principle ู„ูˆ ู†ุนู…ู„ู‡ุง ุนู„ู‰ ู†ุธุงู… ู…ุนุงุฏู„ุงุช ุจูŠุณู‡ู„ ุนู„ูŠู‡
214
00:16:08,520 --> 00:16:12,380
ูƒูŠู ู‡ุฐุง ุงู„ูุญุต ู„ู‡ ุงู„ principleุŸ ุฅู†ู‡ ุนู†ุฏู†ุง amino
215
00:16:12,380 --> 00:16:15,940
acid ุฃู†ุง ุจุถูŠู ุญู…ุถ ุฃู…ูŠู†ูŠุŒ ุจุถูŠู ุนู„ูŠู‡ ู†ูŠู†ู‡ูŠุฏุฑูŠู†ุฑูŠุงุดุง
216
00:16:15,940 --> 00:16:17,240
ุฅูŠุด ุจูŠุนู…ู„ ุงู„