diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/4u9lal8-mc4.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/4u9lal8-mc4.srt new file mode 100644 index 0000000000000000000000000000000000000000..b3e9f445cc5abb631375c46b6a8a8d2aff205e7b --- /dev/null +++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/4u9lal8-mc4.srt @@ -0,0 +1,1157 @@ +1 +00:00:04,890 --> 00:00:07,490 +أعوذ بالله من الشيطان الرجيم بسم الله الرحمن + +2 +00:00:07,490 --> 00:00:13,690 +الرحيم نستكمل إن شاء الله في هذه المحاضرة الحديث + +3 +00:00:13,690 --> 00:00:20,670 +عن العوارض السماوية وكنا قد بدأنا في الحديث عن أول + +4 +00:00:20,670 --> 00:00:24,850 +العوارض السماوية وهو الصغر واليوم إن شاء الله + +5 +00:00:24,850 --> 00:00:29,510 +سنتحدث في هذه المحاضرة عن باقي العوارض السماوية + +6 +00:00:29,510 --> 00:00:37,720 +عندنا عارض الجنون، الجنون هو عبارة عن آفة تصيب + +7 +00:00:37,720 --> 00:00:43,220 +القوة العقلية للإنسان فتؤدي إلى اختلال في القوة + +8 +00:00:43,220 --> 00:00:47,960 +المميزة عنده بحيث يصبح غير قادر على التمييز بين + +9 +00:00:47,960 --> 00:00:53,320 +الأمور الحسنة والقبيحة على نهج العقل السليم + +10 +00:00:53,320 --> 00:01:00,240 +وعندي كمان تعريف هو للجنون طبعا اختلال العقل بحيث + +11 +00:01:00,240 --> 00:01:04,380 +يمنع جريان الأفعال والأقوال على نهج العقل الذي + +12 +00:01:04,380 --> 00:01:07,980 +نادرا طبعا عندك التعريف الأول أو التعريف الثاني + +13 +00:01:07,980 --> 00:01:13,160 +أنت عندك حرية الخيار أنك تحفظه التعريف الأول أو + +14 +00:01:13,160 --> 00:01:20,380 +التعريف الثاني للجنون أما بالنسبة لحكم الجنون فإن + +15 +00:01:20,380 --> 00:01:28,440 +الجنون الأصلي الممتد يسقط التكاليف اتفاقًا وهو مزيل + +16 +00:01:28,440 --> 00:01:34,260 +لأهلية الأداء بنوعيها التي هي أهلية الأداء + +17 +00:01:34,260 --> 00:01:41,660 +المنعدمة أقصد وأهلية الأداء القاصرة وأهلية الأداء + +18 +00:01:41,660 --> 00:01:48,900 +الكاملة فلا يجب عليه شيء من العبادات ولا يصح منه + +19 +00:01:48,900 --> 00:01:54,800 +أي تصرف من التصرفات ولكنه لا ينافي أهلية الوجوب + +20 +00:01:54,800 --> 00:02:01,220 +فيارث ويملك لبقاء ذمته طبعا زي ما شوفنا عندنا + +21 +00:02:01,220 --> 00:02:05,480 +الجنون الأصلي الممتد يسقط التكاليف باتفاق العلماء + +22 +00:02:05,480 --> 00:02:10,340 +لِيش؟ لأنه مزيل لأهلية الأداء ولا يجب عليه أي شيء + +23 +00:02:10,340 --> 00:02:15,360 +من العبادات ولا يصح منه أي تصرف من التصرفات في + +24 +00:02:15,360 --> 00:02:20,780 +المعاملات من عقود وغيرها، ولكنّه لا ينافي أهلية + +25 +00:02:20,780 --> 00:02:25,840 +الوجوب ليش؟ لأن أساس أهلية الوجوب الحياة وحياته + +26 +00:02:25,840 --> 00:02:34,100 +المجنون طبعا موجودة، والجنون العارضي غير الممتد لا + +27 +00:02:34,100 --> 00:02:40,220 +يسقط التكاليف برضه باتفاق العلماء أما خلاف العلماء + +28 +00:02:40,220 --> 00:02:45,540 +في الجنون العارضي بعد البلوغ سواء كان الممتد + +29 +00:02:45,540 --> 00:02:52,150 +والأصلي غير الممتد على أقوال وهذا طبعا مش .. إحنا + +30 +00:02:52,150 --> 00:02:56,630 +مش معنيين أن نفصل هذه الأقوال كل ما علينا نعرف أن + +31 +00:02:56,630 --> 00:03:01,850 +هناك جنون أصلي ممتد وعنا جنون عارضي غير ممتد + +32 +00:03:01,850 --> 00:03:06,130 +الجنون الأصلي الممتد يسقط التكاليف اتفاقًا، والجنون + +33 +00:03:06,130 --> 00:03:11,600 +العارضي غير الممتد لا يسقطوها اتفاقًا أما خلاف + +34 +00:03:11,600 --> 00:03:16,420 +العلماء ده في الجنون العارضي اللي هو بعد البلوغ + +35 +00:03:16,420 --> 00:03:20,960 +الممتد والأصلي غير الممتد طبعا إحنا مش معنيين بفصل + +36 +00:03:20,960 --> 00:03:26,320 +في هذا الخلاف فقط للمعرفة أن في هذه النقطة يوجد + +37 +00:03:26,320 --> 00:03:32,240 +خلاف عند العلماء إذا اللي اللي اللي بدناياهو + +38 +00:03:32,240 --> 00:03:37,380 +والمطلوب هو الجنون الأصلي الممتد والجنون العارضي + +39 +00:03:37,380 --> 00:03:41,160 +غير الممتد اللي هو محل اتفاق عند العلماء أنهم + +40 +00:03:41,160 --> 00:03:48,440 +يسقطون التكاليف باتفاق العلماء طبعا ممكن تسألوا + +41 +00:03:48,440 --> 00:03:53,540 +أنتوا إيش يعني الممتد؟ الممتد هذا يختلف امتداده + +42 +00:03:53,540 --> 00:03:59,820 +بحسب كل عبادة، الامتداد في الصلاة يزيد على يوم + +43 +00:03:59,820 --> 00:04:05,580 +وليلة، والامتداد في الصيام يستغرق شهر رمضان طبعا + +44 +00:04:05,580 --> 00:04:10,380 +الامتداد اللي هو الوقت، وقت الجنون هذا، الجنون هذا + +45 +00:04:10,380 --> 00:04:15,740 +امتداده بحسب كل عبادة، لما نيجي لها الصلاة يزيد + +46 +00:04:15,740 --> 00:04:19,240 +على يوم وليلة، أما بالصيام يستغرق شهر رمضان، أما + +47 +00:04:19,240 --> 00:04:25,380 +الامتداد في الزكاة مفترض آخر واحدة فيها خطأ، التي + +48 +00:04:25,380 --> 00:04:30,280 +هي استغراق الحول، يعني هذا بتصححوها الامتداد آخر + +49 +00:04:30,280 --> 00:04:34,580 +واحدة مش في الصلاة، الامتداد في الزكاة اللي هو إيه؟ + +50 +00:04:34,580 --> 00:04:40,700 +استغراق الحول، يعني مرور الحول كامل على نأتي + +51 +00:04:40,700 --> 00:04:45,260 +الآن للحديث عن العته، العته هو آفة توجب خللًا في + +52 +00:04:45,260 --> 00:04:50,840 +العقل فيصير صاحبه مختلط الكلام، فيشبه بعضه كلام + +53 +00:04:50,840 --> 00:04:54,720 +العقلاء وبعضه كلام المجانين وكذا سائر أموره، + +54 +00:04:54,720 --> 00:04:58,800 +يمكن أقول لكم بتعرفوا ليش أو أدا اللي بيجي في باب + +55 +00:04:58,800 --> 00:05:04,910 +الحارة، يعني تقريبًا هو بيشبه هذه الحالة حكم المعتوه + +56 +00:05:04,910 --> 00:05:10,730 +كالصبي المميز في جميع ما ذكرنا له لو سألتك سؤال ما + +57 +00:05:10,730 --> 00:05:16,610 +هو حكم المعتوه مش هتكتبي لي طبعًا المعتوه كالصبي + +58 +00:05:16,610 --> 00:05:20,570 +المميز لأ في جميع أحوال وما ذكرناه سابقًا بديك + +59 +00:05:20,570 --> 00:05:24,190 +تذكر الأحوال التي ذكرناها للصبي المميز وتكتبيها + +60 +00:05:24,190 --> 00:05:29,830 +هنا في حكم المعتوه وهو اختيار عامة المتأخرين من + +61 +00:05:29,830 --> 00:05:36,420 +الحنفية بمعنى أن له أهلية أداء قاصرة أما بالنسبة لي + +62 +00:05:36,420 --> 00:05:43,980 +النسيان هو غفلة من الإنسان أو غفلة الإنسان وذهوله + +63 +00:05:43,980 --> 00:05:48,760 +عن بعض معلوماته من غير آفة في عقله ولا في تمييزه + +64 +00:05:48,760 --> 00:05:54,600 +وكلنا بننسى هذا أمر يعني موجود عند الإنسان إذا + +65 +00:05:54,600 --> 00:05:59,800 +النسيان هو غفلة الإنسان وذهوله عن بعض معلوماته من + +66 +00:05:59,800 --> 00:06:05,870 +غير آفة في عقله ولا في تمييزه هذه أسماء بتضحك قاعدة + +67 +00:06:05,870 --> 00:06:12,770 +وتقول أنا بخبي الجوال تبعي وأنسى وين خبيته وهذا + +68 +00:06:12,770 --> 00:06:16,630 +النسيان شيء طبيعي موجود في الإنسان وهنا كمان + +69 +00:06:16,630 --> 00:06:21,010 +تعريف هو عدم استحضار الشيء وقت الحاجة اه وفي ناس + +70 +00:06:21,010 --> 00:06:25,950 +هناك كمان بتضحك بتقول يعني كمان في الامتحان بنسى + +71 +00:06:25,950 --> 00:06:30,120 +أنا معلومات كنت درستها ومع أني درستها كويس لكن + +72 +00:06:30,120 --> 00:06:35,440 +سبحان الله عند الامتحان بنساها وساعة ما يخلص وقت + +73 +00:06:35,440 --> 00:06:41,000 +الامتحان بتذكر كل المعلومات التي ما كنتش مستحضراها + +74 +00:06:41,000 --> 00:06:46,400 +وقت الإجابة إذن النسيان هو عدم استحضار الشيء وقت + +75 +00:06:46,400 --> 00:06:51,120 +الحاجة أما بالنسبة لحكم النسيان هو سبب يعجز به + +76 +00:06:51,120 --> 00:06:56,350 +الإنسان عن الأداء كسائر الأعذار ولكنه لا يمنع وجوب + +77 +00:06:56,350 --> 00:07:01,330 +الحقوق لعدم إخلاله بأهلية الإنسان وهو عذر عارضي + +78 +00:07:01,330 --> 00:07:09,850 +يغير بعض الأحكام ولا يسقطها طيب نسأل الآن سؤال ما + +79 +00:07:09,850 --> 00:07:14,330 +هو تأثير النسيان مزال إحنا حكينا لا يمنع أهلية + +80 +00:07:14,330 --> 00:07:19,550 +الوجوب ولا أهلية الأداء طيب ففي ماذا يؤثر تعالوا + +81 +00:07:19,550 --> 00:07:24,790 +نجاوب مع بعض على هذا السؤال الإجابة هنقسمها لقسمين + +82 +00:07:24,790 --> 00:07:29,930 +قسم الأول في حقوق العباد في حقوق العباد لا يصح أن + +83 +00:07:29,930 --> 00:07:33,990 +يعتبر نسيان عذرًا فكل تصرف يصدر من الشخص من بيع + +84 +00:07:33,990 --> 00:07:38,870 +وشراء ورهن وإجارة وغيره تترتب عليه الآثار الشرعية + +85 +00:07:38,870 --> 00:07:44,350 +فلا يصح الاعتذار بأنه كان ناسيا غافلًا لأن حقوق + +86 +00:07:44,350 --> 00:07:48,930 +الناس محترمة لحاجة الناس إليها وفتح هذا الباب يؤدي + +87 +00:07:48,930 --> 00:07:55,940 +إلى ضياع حقوق الناس وعرض تصرفاتهم للعبث والاضطراب + +88 +00:07:55,940 --> 00:08:00,600 +أما في حقوق الله تعالى فالنسيان يكون فيها عذرًا + +89 +00:08:00,600 --> 00:08:04,660 +بإيش بالنسبة له ترتب الإثم القول النبي صلى الله + +90 +00:08:04,660 --> 00:08:09,140 +عليه وسلم رفع عن أمتي الخطأ والنسيان طبعًا مش الخطأ + +91 +00:08:09,140 --> 00:08:13,000 +المرفوع الذي هو فيه تقدير محذوف هنا الذي هو إثم + +92 +00:08:13,000 --> 00:08:18,580 +الخطأ تمامًا وهو عند عدم التقصير من الشخص يعني نسيان + +93 +00:08:18,580 --> 00:08:24,240 +يكون عذرًا عند إيه؟ عند عدم التقصير من الشخص وذلك في + +94 +00:08:24,240 --> 00:08:30,760 +الحالات التي ليس فيها ما يذكره بالعبادة كأكل صائم + +95 +00:08:30,760 --> 00:08:35,620 +وشرب ونسيان لأن طبيعة الإنسان تنزع إلى الأكل و + +96 +00:08:35,620 --> 00:08:40,920 +الشرب كثير في أول شهر رمضان وهي كلكوا بتحكوا إنه + +97 +00:08:40,920 --> 00:08:47,240 +كثير أول ما نبدأ الصيام بننسى وبناكل أو بننسى و + +98 +00:08:47,240 --> 00:08:52,090 +بنشرب وهن ربنا سبحانه وتعالى في هذا الحق سامحنا فيه + +99 +00:08:52,090 --> 00:08:57,810 +لِيش؟ لأن ما فيش حاجة بتذكرنا أننا صايمين ليش؟ لأن + +100 +00:08:57,810 --> 00:09:02,090 +طبيعة الإنسان يعني بيأكل وبيشرب ولما يبدأ يصوم + +101 +00:09:02,090 --> 00:09:06,710 +بينسى وبيفكر حاله أنه مش صائم أنا نفسي أنسى في + +102 +00:09:06,710 --> 00:09:11,710 +رمضان ولا مرة نسيت الحمد لله أما إذا كان النسيان + +103 +00:09:11,710 --> 00:09:17,410 +عَمْ تقصير فلا يكون عذرًا كيف يعني؟ كالأكل في الصلاة + +104 +00:09:17,410 --> 00:09:20,650 +بنفع الواحد هو يصلي يجيب ساندوتش ويقعد يأكل؟ لأ + +105 +00:09:20,650 --> 00:09:24,030 +أصلًا مش عادة الإنسان أنه عندما يبدأ يصلي يشرب أو + +106 +00:09:24,030 --> 00:09:31,150 +يأكل فوجود هيئة الصلاة تعتبر مذكر للإنسان أنه لا + +107 +00:09:31,150 --> 00:09:37,190 +يصح له الأكل والشرب في هذه الحالة إذن النسيان يكون + +108 +00:09:37,190 --> 00:09:44,010 +عذرًا في حقوق الله تعالى والنسيان لا يكون عذرًا عند + +109 +00:09:44,010 --> 00:09:49,070 +التقصير يعني عند عدم التقصير يكون عذرًا كمان زي ما + +110 +00:09:49,070 --> 00:09:53,410 +حكينا في الصيام لكن عند عدم التقصير عند التقصير لا + +111 +00:09:53,410 --> 00:09:57,470 +يكون عذرًا كالأكل في الصلاة فلا يعتد به الوجود + +112 +00:09:57,470 --> 00:10:03,390 +المذكر وهو هيئة الصلاة نلاحظ الآن حقوق الله تعالى + +113 +00:10:03,390 --> 00:10:08,850 +شرعت للابتلاء فتحتاج إلى قصد والنسيان يعدم القصد + +114 +00:10:08,850 --> 00:10:13,800 +فيكون فيكون عذرًا زي ما حكينا في المفارقة السابقة + +115 +00:10:13,800 --> 00:10:18,860 +نأتي الآن إلى النوم طبعًا كلنا بنحكي النوم فروحتوا + +116 +00:10:18,860 --> 00:10:23,360 +كلكوا إيه؟ اتذكرتوا النوم ونعستوا؟ لأ صح صحوا + +117 +00:10:23,360 --> 00:10:28,800 +معايا وخلينا نستمع إلى الأحكام التي تتعلق بالنوم + +118 +00:10:28,800 --> 00:10:33,760 +أول حاجة تعريف النوم زي ما اتعودنا في كل العوارض + +119 +00:10:33,760 --> 00:10:39,650 +زي ما اتفقنا في الأول أنك هتعرفي التعريف العارض وكل + +120 +00:10:39,650 --> 00:10:44,110 +ما يتعلق به من أحكام طبعًا الأحكام التي تؤثر على + +121 +00:10:44,110 --> 00:10:47,850 +أهلية المكلف سواء أهلية الوجوب أو أهلية الأداء + +122 +00:10:47,850 --> 00:10:52,430 +النوم هو فطور طبيعي يحدث في الإنسان باختيار منه + +123 +00:10:52,430 --> 00:10:58,890 +وتمنع الحواس الظاهرة والباطنة عن العمل مع سلامتها + +124 +00:10:58,890 --> 00:11:06,470 +وتمنع استعمال العقل مع بقائه يعني أثناء النوم لأ + +125 +00:11:06,470 --> 00:11:11,790 +العقل ما بيشتغلش والحواس كمان الظاهرة والباطنة + +126 +00:11:11,790 --> 00:11:19,070 +لا تعمل طبعا مع وجود هذه الحواس، النوم طبعا لا يصيب + +127 +00:11:19,070 --> 00:11:24,710 +الإنسان بخلل في ذمته وسلامته، إذ كلاهما باقٍ لديه + +128 +00:11:24,710 --> 00:11:28,830 +ولكنه عارض يحول بينه وبين الأداء، حالة النوم + +129 +00:11:28,830 --> 00:11:33,030 +لامتناع الفهم من ناحية وعدم إمكان إيجاد الفعل من + +130 +00:11:33,030 --> 00:11:37,440 +جهة أخرى، يعني لو الإنسان نام وقدّن الظهر وإجا يقدّن + +131 +00:11:37,440 --> 00:11:43,620 +العصر وهو يعني نايم، هل يستطيع أداء الصلاة وهو + +132 +00:11:43,620 --> 00:11:48,620 +نايم؟ لا، لإيش؟ لأنه منع الفهم ومنع إمكان أصلا + +133 +00:11:48,620 --> 00:11:56,460 +إيجاد الفعل، وهو إيه؟ وهو نايم الآن، بالنسبة لحكم + +134 +00:11:56,460 --> 00:12:01,460 +النائم، يستلزم تأخير مخاطبة النائم بالأداء حتى + +135 +00:12:01,460 --> 00:12:05,640 +يستيقظ ويتمكن من أداء الفعل حقيقة بالانتباه قبل + +136 +00:12:05,640 --> 00:12:09,860 +الفوات، وإن لم ينتبه، لازم يلقيا به عن طريق القضاء + +137 +00:12:09,860 --> 00:12:13,280 +لقول النبي صلى الله عليه وسلم: إنه ليس في النوم + +138 +00:12:13,280 --> 00:12:19,260 +تفريط، إنما تفريطه في اليقظة، فإذا نسي أحدكم صلاة أو + +139 +00:12:19,260 --> 00:12:24,920 +نام عنها فليؤدها إذا ذكرها، ولقوله عز وجل: "وأقم الصلاة لذكري" + +140 +00:12:24,920 --> 00:12:30,170 +ولذلك فإنما يصدر عن النائم من عبادات + +141 +00:12:30,170 --> 00:12:34,490 +فلا يعتبر فيها القصد والإرادة، فيعتبر باطلا، يعني لو + +142 +00:12:34,490 --> 00:12:38,530 +كنت بتحلم أنك بتصلي، الصلاة هذه غير صحيحة لأنها لا + +143 +00:12:38,530 --> 00:12:45,590 +فيها نية ولا فيها قصد، فتكون أيضا باطلة، هذه في + +144 +00:12:45,590 --> 00:12:51,650 +العبادات، أما في المعاملات لو الإنسان هو نايم، حلم + +145 +00:12:51,650 --> 00:12:59,910 +أنه ببيع البيت أو ببيع السيارة، فهذا طبعا بيعه غير + +146 +00:12:59,910 --> 00:13:06,370 +صحيح، لأنه ما في قصد ولا إرادة صحيحة لهذا البيع + +147 +00:13:06,370 --> 00:13:11,370 +وكذلك الشراء، وكذلك الرد والإسلام والعتق والطلاق + +148 +00:13:11,370 --> 00:13:15,990 +كله لفقد النائم للاختيار والإرادة، وقول النبي صلى + +149 +00:13:15,990 --> 00:13:21,230 +الله عليه وسلم: "رفع القلم عن ثلاثة، ومنهم النائم" فإن + +150 +00:13:21,230 --> 00:13:25,170 +النائم لا يسأل عن أفعاله ولا عن تصرفاته إلا ما + +151 +00:13:25,170 --> 00:13:29,050 +كان من باب الإتلاف لمال الغير أو الاعتداء عليه + +152 +00:13:29,050 --> 00:13:33,870 +فيلزمه الضمان في ماله، للتعلق حق الغير به ورفع + +153 +00:13:33,870 --> 00:13:38,070 +الدرر عنه، يعني بصحّش أنه يقول: والله كنت نايم فأنا + +154 +00:13:38,070 --> 00:13:42,750 +مسكت جوال إسراء ورميته من الشبك، وبقولها: آسفة والله كنت نايمة مش صاحية، وأنا بمشي وأنا نايمة طبعا + +155 +00:13:42,750 --> 00:13:46,380 +على طول عليا إيه؟ يلزمني الضمان في المال، وأرجعلها + +156 +00:13:46,380 --> 00:13:50,960 +بدل الجوال أو أدفع قيمة هذا الجوال، طبعا + +157 +00:13:50,960 --> 00:13:58,400 +في بعض يعني المسائل قرروها العلماء، انقلاب النائم + +158 +00:13:58,400 --> 00:14:04,720 +على غيره انقلابا يؤدي إلى قتله، يلزمه بالدية كالقتل + +159 +00:14:04,720 --> 00:14:10,560 +الخطأ، ولا يلزمه القصاص، لنعدام الإرادة والقصد لدى + +160 +00:14:10,560 --> 00:14:15,440 +النائم، نجي الآن لعارض الإغماء، انتبهوا عاد في عارض + +161 +00:14:15,440 --> 00:14:21,460 +الإغماء، منيح عشان تشوف إيش الأحكام اللي بتتعلق في + +162 +00:14:21,460 --> 00:14:25,780 +المغمي عليها، طبعا هذا من العوارض السماوية، والإغماء + +163 +00:14:25,780 --> 00:14:31,290 +هو ذا معطل لقوة الإدراك عند الإنسان مما يجعله + +164 +00:14:31,290 --> 00:14:36,310 +عاجزا عن استعمال قواه العقلية، وإن كان العقل موجودا + +165 +00:14:36,310 --> 00:14:41,750 +لأن عاجزة عن استعمال الشيء لا يعني عدم وجوده + +166 +00:14:41,750 --> 00:14:48,550 +بالنسبة لي، حكم الإغماء هو عارض يمنع فهم الخطاب حال + +167 +00:14:48,550 --> 00:14:53,070 +وقوعه، مش معقول المغمي عليه يكون بيفهم، ولا يمنع + +168 +00:14:53,070 --> 00:14:57,170 +أهلية الوجوب، لأنه يتنفس وحي وموجود، لأن الأهلية + +169 +00:14:57,170 --> 00:15:01,630 +تبقى ببقاء الحياة، ولكنه يفقد أهلية الأداء عند + +170 +00:15:01,630 --> 00:15:05,250 +الوقوع، فلا يطالب بالتكاليف كلها، حالة غيبوبة فلا + +171 +00:15:05,250 --> 00:15:12,270 +تلزمه الصلاة ولا الصيام، وإن لازمه ذلك + +172 +00:15:12,270 --> 00:15:20,010 +بعد زوالها، يعني بعد زوال عارض الإغماء، ولهذا + +173 +00:15:20,410 --> 00:15:25,410 +فالإغماء عارض مؤخر لأحكام الأهلية، يعني وقت وقوعه + +174 +00:15:25,410 --> 00:15:30,830 +ما بيقدرش المغمي عليه ينفذ الصلاة، لكن مطالب بيها + +175 +00:15:30,830 --> 00:15:35,030 +وبأدائها بعد زوال حالة الإغماء، بيكون طبعا أداءه + +176 +00:15:35,030 --> 00:15:40,870 +إذا كان في حالة في وجود وقت الصلاة، بيكون أداء، أما + +177 +00:15:40,870 --> 00:15:45,210 +في أول صيام طبعا، أما في حالة خروج الوقت فبيكون قضاء + +178 +00:15:45,210 --> 00:15:53,460 +بالنسبة لي، القضاء فإن الإغماء يختلف طولا وقصرا، فإن + +179 +00:15:53,460 --> 00:16:00,140 +كان قصيرا فحكم النوم لا يسقط القضاء، وإن كان طويلا + +180 +00:16:00,140 --> 00:16:05,720 +سقط القضاء به، كما يسقط بالجنون والصغر والحيض + +181 +00:16:05,720 --> 00:16:11,020 +والنفاذ، بخصوص الصلاة، نقطة الآن للحديث عن المرض، صار + +182 +00:16:11,020 --> 00:16:17,450 +أنتوا فاهمين أنه كل عارض من هذه العوارض، نعرفه و + +183 +00:16:17,450 --> 00:16:21,730 +نشوف الأحكام اللي بتتعلق فيه، وأنتِ عليك زي ما حكيت + +184 +00:16:21,730 --> 00:16:25,970 +في الأول، بدك تجيبي ورقة وتسجلي كل عارض من هذه + +185 +00:16:25,970 --> 00:16:30,010 +العوارض، تسجلي الأحكام اللي بتتعلق فيه مع تعريفه + +186 +00:16:30,010 --> 00:16:33,310 +تدرسيهم، وهيك بسهّل عليك أمر الدراسة واستيعاب + +187 +00:16:33,310 --> 00:16:38,490 +الموضوع، وأي سؤال بعد ما أتحدث عن كل العوارض، هنعمل + +188 +00:16:38,490 --> 00:16:44,060 +محاضرة إن شاء الله online عشان نجاوب على كل + +189 +00:16:44,060 --> 00:16:48,560 +الأسئلة اللي بتتعلق بهذا الموضوع، عشان هيك إذا + +190 +00:16:48,560 --> 00:16:51,900 +اعترض إليك أي سؤال، بتسجليه في ال note عندك سواء + +191 +00:16:51,900 --> 00:16:56,720 +note الجوال أو ال note اللي عندك في الدفتر أو غيره + +192 +00:16:56,720 --> 00:17:02,680 +وتسأليني يا إن شاء الله في المحاضرة اللي هتكون + +193 +00:17:02,680 --> 00:17:06,540 +قريبة بإذن الله، دعانًا، بالنسبة للمرض هو حالة غير + +194 +00:17:06,540 --> 00:17:12,880 +طبيعية تصيب بدن الإنسان فتوهن قواه وتخرجه عن حاله + +195 +00:17:12,880 --> 00:17:18,880 +الطبيعي، إذ هو حال يزول به اعتدال الطبيعة عند + +196 +00:17:18,880 --> 00:17:25,020 +الإنسان، بالنسبة لحكم المرض، المرض لا ينافي لأهلية + +197 +00:17:25,020 --> 00:17:30,140 +الأجوبة ولا أهلية الأدواء، سواء كانت هذه الأهلية مما + +198 +00:17:30,140 --> 00:17:33,440 +يتعلق بحقوق الله تعالى أو حقوق العباد، لأنه لا يزيل + +199 +00:17:33,440 --> 00:17:36,920 +قدرة المرض، لا الظاهرة ولا الباطنة، ولا يترتب عليه + +200 +00:17:36,920 --> 00:17:41,140 +فساد في الذمة أو العقل أو النطق، بما أن المرض من + +201 +00:17:41,140 --> 00:17:44,700 +أسباب العجز، فقد رخصها أو رخصة للمريض بالفطر وقضاء + +202 +00:17:44,700 --> 00:17:50,270 +الفرض بعد زوال المرض، لقول الله تعالى: "فمن كان منكم مريضا أو على سفر فعدد من أيام أخر" وشرعت الصلاة + +203 +00:17:50,270 --> 00:17:54,990 +على المريض بقدر ما يتمكن به من الأداء، فيصل قاعدا + +204 +00:17:54,990 --> 00:18:00,210 +أو مستلقيا أو مضجعا أو على جنب أو حسب مقدرته، ولو + +205 +00:18:00,210 --> 00:18:05,070 +كان إيماء لطيفا، أما إذا اتصل المرض بالموت، فكان + +206 +00:18:05,070 --> 00:18:09,970 +عارضا تتغير به بعض الأحكام، فلا تصح مثلا هبته ولا + +207 +00:18:09,970 --> 00:18:16,350 +تبرعه إلا في حدود الثرثرة طبعا، اللي بيحدد الأطباء هم + +208 +00:18:16,350 --> 00:18:21,210 +اللي بيحددوا إذا كان هذا المرض متصل بالموت، وهذا + +209 +00:18:21,210 --> 00:18:26,870 +علمه عند الله، لكن في بعض الإشارات الأطباء يعلمونها + +210 +00:18:26,870 --> 00:18:29,890 +أنها هذا المرض يؤدي إلى الوفاة، فإذا تصرف المريض مرض + +211 +00:18:29,890 --> 00:18:34,490 +الموت متصل بالوفاة يعني، وأصدر بعض الأحكام، فهذه + +212 +00:18:34,490 --> 00:18:44,340 +الأحكام لا تصح منه، نجي الآن لعارض الحيض والنفساء + +213 +00:18:44,340 --> 00:18:49,780 +الحيض هو دم ينفضه رحم المرأة السليمة عن الدأي + +214 +00:18:49,780 --> 00:18:58,920 +والصغر، يعني الدم هذا بيخرج من المرأة الطبيعية في كل + +215 +00:18:58,920 --> 00:19:03,840 +شهر لا عن مرض ولا في حالة الصغر، النفاس هو الدم + +216 +00:19:03,840 --> 00:19:08,020 +الخارج من قبل المرأة بعد الولادة، بالنسبة لي حكم + +217 +00:19:08,020 --> 00:19:14,100 +الحيض والنفاس، طبعا وإن كان دم الحيض يختلف عن أو + +218 +00:19:14,100 --> 00:19:19,540 +سبب خروج دم الحيض يختلف عن سبب خروج دم النفاس، إلا + +219 +00:19:19,540 --> 00:19:25,760 +أنهم مشتركان في الصورة والحكم، إذ كلاهما لا يثبت + +220 +00:19:25,760 --> 00:19:34,960 +أهلية الوجوب ولا الأداء، لبقاء الذمة والعقل وقوة + +221 +00:19:34,960 --> 00:19:39,380 +البدن، إلا أنهم يثبت بالنص أنهم من النجاسات، أي + +222 +00:19:39,380 --> 00:19:43,180 +أنهم مبطلان بأنفسهم، ولما كانت الطهارة شرطا + +223 +00:19:43,180 --> 00:19:48,660 +لأداء العبادات، وفي فوات هذا الشرط فوات الأداء، إلا + +224 +00:19:48,660 --> 00:19:53,540 +أنهم قد دلت نصوص على عدم وجوب قضاء الصلاة على + +225 +00:19:53,540 --> 00:19:58,290 +الحائض والنفساء، دفعا للحرج، لأنه حيكون عليها صلوات + +226 +00:19:58,290 --> 00:20:03,290 +كتيرة، حيصير الأمر يعني في مشقة للحائض والنفساء + +227 +00:20:03,290 --> 00:20:08,070 +فالمشرع عنه بدرق هذه المشقة، مع طول الزمن وتكرار + +228 +00:20:08,070 --> 00:20:13,590 +الصلاة، في حين دلت النصوص على وجود قضاء الصوم على + +229 +00:20:13,590 --> 00:20:19,270 +الحائض والنفساء، وذلك لعدم الحرش في قضائه بالنسبة + +230 +00:20:19,270 --> 00:20:24,170 +لهم، فإن الحيض لا يستغرق الشهر كله، ووقوع النفساء من + +231 +00:20:24,170 --> 00:20:27,810 +النوادر التي لا يبنى عليها حكم، عليها نيجي الآن + +232 +00:20:27,810 --> 00:20:32,350 +لآخر العوارض السماوية وهو الموت، طبعا الموت يعتبر + +233 +00:20:32,350 --> 00:20:38,190 +من أهم عوارض الأهلية، لأنه عجز كامل لا تشبه أي قدرة + +234 +00:20:38,190 --> 00:20:45,330 +ولذلك كان منافيا لجميع أنواع الأهلية، سواء كانت + +235 +00:20:45,330 --> 00:20:51,750 +أهلية الأداء أو أهلية الوجوب، فالموت هو انقطاع تعلق + +236 +00:20:51,750 --> 00:20:57,890 +الروح بالبدن ومفارقته، وتبدو الحال وانتقال من + +237 +00:20:57,890 --> 00:21:04,610 +دار إلى دار، بالنسبة لحكم الموت طبعا الموت منافي + +238 +00:21:04,610 --> 00:21:09,150 +لأهلية أحكام الدنيا من كل ما فيه تكليف، من أجل ذلك + +239 +00:21:09,150 --> 00:21:15,910 +تسقط جميع العبادات عن الميت كالصلاة والذكاة والصوم + +240 +00:21:15,910 --> 00:21:21,610 +والحج، خلاص انتهت الحياة، هدمت هذه الحياة، فبالتالي + +241 +00:21:21,610 --> 00:21:25,610 +تسقط جميع العبادات ويبقى عليه اسم قصر فيه أو تركه + +242 +00:21:25,610 --> 00:21:32,590 +في الدنيا، وهذا عند الله تعالى طبعا، اسم قصر فيه أو + +243 +00:21:32,590 --> 00:21:37,510 +تركه من تكاليف بلا عذر شرعي، والأحكام بالنسبة للميت + +244 +00:21:37,510 --> 00:21:42,450 +نوعا أحكام أخروية تتعلق بالآخرة، وأحكام إيه؟ أحكام + +245 +00:21:42,450 --> 00:21:48,730 +دنيوية، أما بالنسبة لأحكام الأخروية، ما يجب له على + +246 +00:21:48,730 --> 00:21:55,330 +الغير من الحقوق والمظالم التي تقول إلى النفس + +247 +00:21:55,330 --> 00:22:00,150 +والعرض، ما يجب عليه من الحقوق والمظالم، ما يلقاه من + +248 +00:22:00,150 --> 00:22:04,470 +الثواب والكرام بوسط الإيمان، وما يكسبه من طعام كله + + + +251 +00:22:15,970 --> 00:22:21,290 +طبعا هذا أحكام في الآخرة وما يلقاه من عقاب لتقصيره + +252 +00:22:21,290 --> 00:22:25,350 +في القيام بالعبادات وفعله لـ المعاصر كل هذه الأحكام + +253 +00:22:25,350 --> 00:22:28,810 +الأخروية عند الله سبحانه وتعالى احنا في الدنيا + +254 +00:22:28,810 --> 00:22:32,310 +مالناش دعوة فيها أما الأحكام الدنياوية اللي هي + +255 +00:22:32,310 --> 00:22:36,250 +منها ما كان من باب التكليف وهذا يسقط عن الميت + +256 +00:22:36,250 --> 00:22:41,650 +بمجرد موته ولا يبقى عليه اسم إلا إثم ما قصر فيه + +257 +00:22:41,650 --> 00:22:48,750 +لأن الإثم من أحكام الآخرة أما ما كان مشروعا لحاجة + +258 +00:22:48,750 --> 00:22:53,490 +غير الميت وهذا القسم منوعين أما أن يكون حقا متعلق + +259 +00:22:53,490 --> 00:22:58,170 +بالعين يعني في وديعة عند الميت والحاجات المرصودة + +260 +00:22:58,170 --> 00:23:03,140 +والمأجورة والمقصودة والأمانات وفيه إما أن يكون + +261 +00:23:03,140 --> 00:23:07,500 +أن يتعلق بالدين أما إذا كان متعلق بالعين فإنه يبقى + +262 +00:23:07,500 --> 00:23:10,840 +ببقاء العين لتعلق الحق فيها بذات العين لا بفعل + +263 +00:23:10,840 --> 00:23:16,640 +مكلف وحقوق العباد تتعلق بالمال أما الدين فلا يبقى + +264 +00:23:16,640 --> 00:23:21,720 +بمجرد الذمة لأن الذمة هي انضعفت وخربت بالموت ولكنه + +265 +00:23:21,720 --> 00:23:26,480 +يبقى إذا تقوت الذمة بمال تركه الميت أو كفيل بالدين + +266 +00:23:26,480 --> 00:23:32,860 +قبل الموت يعني إذا كان الميت عليه دين، الدين هذا + +267 +00:23:32,860 --> 00:23:37,940 +ينتقل من ذمته لأنها أصلا مات وما فيش له ذمة الآن، + +268 +00:23:37,940 --> 00:23:41,720 +ينتقل من الذمة ويتعلق بماله اللي هو مال تركته + +269 +00:23:41,720 --> 00:23:47,240 +الميت فيقضى منه أو إذا كان له كفيل بهذا المال، + +270 +00:23:47,240 --> 00:23:52,600 +طبعا هذا الكفيل كان موجود قبل الموت، بقى واجب عليه + +271 +00:23:52,600 --> 00:23:57,120 +لحاجة تغيره مشروعا بطريق الصلة وهذا الواجب طبعا + +272 +00:23:57,120 --> 00:24:03,620 +ينتهي بالموت لأن الموت أنهى الذمة عصفها بالذمة + +273 +00:24:03,620 --> 00:24:10,700 +فمنع وجوب الصلاة مثال صدقة الفطر والزكاة ونفقة + +274 +00:24:10,700 --> 00:24:16,490 +المحارم إلا أن يوصي فتصح وصيته لأن الشرع أجاز ذلك + +275 +00:24:16,490 --> 00:24:22,150 +فتنفذ وصيته في حدود الثلث أما ما شرع للميت بناء + +276 +00:24:22,150 --> 00:24:26,830 +على حاجته له يبقى له مقدار ما يندفع به هذه الحاجة + +277 +00:24:26,830 --> 00:24:31,170 +كأفراد بقاء التركة على حكم ملك الميت حتى تؤدى + +278 +00:24:31,170 --> 00:24:38,660 +منها الحقوق وهي مؤن التجهيز والدفن وقضاء الديون ثم + +279 +00:24:38,660 --> 00:24:42,700 +تنفذ الوصية في حدود ثلث التركة يعني تقوم التركة + +280 +00:24:42,700 --> 00:24:48,180 +مكان ذمة الميت كأنه الميت يعني حيوة له وجود وهذا + +281 +00:24:48,180 --> 00:24:52,840 +الحياة والوجود تعلقت بالمال ولا بتتعلق بالروح عشان + +282 +00:24:52,840 --> 00:24:59,250 +هيك بيبدوا ينفذوا ما يتعلق أو ما يحتاج له الميت بعد + +283 +00:24:59,250 --> 00:25:05,230 +موته كمقنط تجهيزه ودفنه وقضاء ديونه وتنفيذ وصيته + +284 +00:25:05,230 --> 00:25:10,610 +في حدود ثلث التاريخ نكتفي بهذا القدر في هذه + +285 +00:25:10,610 --> 00:25:17,870 +المحاضرة وكنا قد تحدثنا فيها عن العوارض السماوية + +286 +00:25:18,430 --> 00:25:21,190 +وزي ما حكينا هيك مطلوب منكم بيكون عشر أوراق كل + +287 +00:25:21,190 --> 00:25:27,310 +ورقة مكتوب فيها ما يتعلق بكل عارض من العوارض في + +288 +00:25:27,310 --> 00:25:31,070 +المحاضرة القادمة إن شاء الله سنتحدث عن العوارض + +289 +00:25:31,070 --> 00:25:37,310 +المكتسبة وننهي هذه المحاضرة بإذن الله تعالى على + +290 +00:25:37,310 --> 00:25:41,390 +أمل أن ألقاكم في المحاضرة القادمة بإذن الله تعالى + +291 +00:25:41,390 --> 00:25:44,010 +والسلام عليكم ورحمة الله وبركاته diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/8y70ll-Ykzg_raw.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/8y70ll-Ykzg_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..9f00ee90654c1e95f5506bebd9b8eee9703254d1 --- /dev/null +++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/8y70ll-Ykzg_raw.srt @@ -0,0 +1,792 @@ +1 +00:00:08,000 --> 00:00:10,560 +أعوذ بالله من الشيطان الرجيم بسم الله الرحمن + +2 +00:00:10,560 --> 00:00:14,200 +الرحيم الحمد لله الواحد الأحد أفرز الصمت الذي لم + +3 +00:00:14,200 --> 00:00:18,040 +يلد ولم يولد ولم يكن له غفور أحد والصلاة والسلام + +4 +00:00:18,040 --> 00:00:23,220 +على خير الأنام محمد وعلى آله وصحبه الكرام وبقى + +5 +00:00:23,220 --> 00:00:27,420 +بداية أخوات الحبيبات وبنات الطيبات أحييكم بتحية + +6 +00:00:27,420 --> 00:00:31,100 +طيبة مباركة من عند الله السلام عليكم ورحمة الله + +7 +00:00:31,100 --> 00:00:34,960 +وبركاته اللهم علمنا ماينفعنا وانفعنا بما علمتنا + +8 +00:00:34,960 --> 00:00:40,910 +وزدنا علما اللهم آميننستكمل ما بدأناه في المحاضرة + +9 +00:00:40,910 --> 00:00:46,010 +السابقة والحديث عن العوارض المكتسبة، اليوم ان شاء + +10 +00:00:46,010 --> 00:00:51,930 +الله هنتكلم عن عارض السفر، طبعا السفر هو الخروج + +11 +00:00:51,930 --> 00:00:58,310 +المديد، الطويل يعني، وادناه ثلاثة أيام ولياليها + +12 +00:00:59,380 --> 00:01:07,800 +يقدر بالمقادير المعاصرة 88 كم عند الأحداث 96 كم + +13 +00:01:07,800 --> 00:01:11,940 +السفر طبعا لا ينافي الأهلية سواء كانت أهلية الوجوب + +14 +00:01:11,940 --> 00:01:17,720 +أو أهلية الأداء ولا يمنع شيئا من الأحكام لكنه جعله + +15 +00:01:17,720 --> 00:01:21,740 +في الشرعي من أسباب التخفيف بنفسه مطلقا من غير نظر + +16 +00:01:21,740 --> 00:01:28,680 +إلى مشقة أو عدمهايعني هذا السفر سبب لتخفيف أعباء + +17 +00:01:28,680 --> 00:01:35,300 +بعض العبادات، تمام؟ لكن هذا السفر سواء كان فيه + +18 +00:01:35,300 --> 00:01:40,100 +متعب، يعني مشقة أو مافيش فيه مشقة، مافيش فيه تعب، + +19 +00:01:40,100 --> 00:01:44,780 +سيئا، يعني لو المسافر طلع في طيارة، درجة أولى، + +20 +00:01:44,780 --> 00:01:48,160 +برضه بدنا نسميه مسافر، لو المسافر طلع ماشي على + +21 +00:01:48,160 --> 00:01:51,500 +رجلي، برضه بدنا نسميه مسافر، سواء كان فيه مشقة أو + +22 +00:01:51,500 --> 00:01:56,750 +غير .. أو ماكانش في السفر هذا مشقةويثبت حق السفر + +23 +00:01:56,750 --> 00:02:00,710 +لمن سافروا مجرد إنشاء السفر بعد الخروج من عمران + +24 +00:02:00,710 --> 00:02:04,230 +البلد كما هو معروف في السنة لأن الرسول صلى الله + +25 +00:02:04,230 --> 00:02:09,930 +عليه وسلم لما خرجا للسفر رخص للمسافرين بمجاوزاتهم + +26 +00:02:09,930 --> 00:02:16,550 +العمران يعني مجرد ما يتجاوزوا العمران بطبعا + +27 +00:02:16,550 --> 00:02:21,350 +المسافر اللي حددوها العلماء بيبدأ يعني يطلق عليه + +28 +00:02:21,350 --> 00:02:26,940 +اسم مسافروليس من اللازم اتمامين اليوم او تلاثة + +29 +00:02:26,940 --> 00:02:31,220 +أيام عند القائلين بذلك لأن الرخصة قصد بها الترفيه + +30 +00:02:31,220 --> 00:02:38,400 +في جميع مدة السفر نقتل الآن للعارض القبل الأخير + +31 +00:02:38,400 --> 00:02:45,980 +وهو الجهل الجهل ضد العلم عند تصوره واحتماله أما عن + +32 +00:02:45,980 --> 00:02:50,720 +أثر الجهل في أهلية الإنسانالجهل لا ينافي أهلية + +33 +00:02:50,720 --> 00:02:57,920 +المكلف، لا أهلية الوجوب ولا الأداء إذ أنه متعلق + +34 +00:02:57,920 --> 00:03:05,200 +الأهلين للأهليتين هو الحياة والعقل والتمهيز طيب + +35 +00:03:05,200 --> 00:03:11,500 +خلينا نشوف الجهل يعتبر من الأمور الأصلية في المكلف + +36 +00:03:11,500 --> 00:03:17,900 +يعني بنولدش الإنسان متعلملكنه اعتبره المشرع من + +37 +00:03:17,900 --> 00:03:22,340 +العوارض، ليش؟ لأنه أمر زائد على حقيقة الإنسان + +38 +00:03:22,340 --> 00:03:28,800 +وثابت في حال كالصغر طيب السؤال اللي بيترح نفسه لان + +39 +00:03:28,800 --> 00:03:34,240 +لماذا اعتبر الجهل من العوارض المكتسبة؟ طبعا هذه + +40 +00:03:34,240 --> 00:03:38,880 +نقطة مهمة و يتم السؤال فيها ليش اعتبرنا عارض مكتسب + +41 +00:03:38,880 --> 00:03:45,570 +مع أنه أمر أصلي في الإنسان؟أه الإجابة صحيحة لأن + +42 +00:03:45,570 --> 00:03:51,330 +إزالته باكتساب العلم في قدرة المكلف فكأنه ترك + +43 +00:03:51,330 --> 00:03:56,070 +تحصيل العلم منه اختيارا بمنزلة اكتساب الجهل + +44 +00:03:56,070 --> 00:04:02,410 +باختيار إبقائه فكان مكتسبا من هذا الوجب ولأن العلم + +45 +00:04:02,410 --> 00:04:06,090 +نعمة من الله سبحانه وتعالى متنى بيها على عباده بعد + +46 +00:04:06,090 --> 00:04:14,060 +أن خلقهم وهم لا يعلمون شيءطبعا عندنا آيات كتير، + +47 +00:04:14,060 --> 00:04:20,000 +آيات كريمة بتدل على هذا الأمر لذلك فإن الجهل في + +48 +00:04:20,000 --> 00:04:25,700 +عمومه من العوارض المكتسبة وحددنا هنا السببين ثابت + +49 +00:04:25,700 --> 00:04:29,600 +في حال دون حال، سبب الأول والثاني لأن إزالته + +50 +00:04:29,600 --> 00:04:34,780 +باكتساب العلم في قدرة العبدفكأنه ترك تحصيل العلم + +51 +00:04:34,780 --> 00:04:40,660 +منه بمنزلة اكتساب الجهل باختياري بابقى يعني + +52 +00:04:40,660 --> 00:04:45,820 +الإنسان قادر على أن يتعلم و يزيل هذا الجهل لذلك + +53 +00:04:45,820 --> 00:04:51,760 +كان سببا في جعل الجهل من العوارض الممتزة أما مع + +54 +00:04:51,760 --> 00:04:59,400 +العارض الأخير وهو الإكره طبعا الإكره يعني الإجبار + +55 +00:05:00,200 --> 00:05:05,540 +إجبار الغير أو حمل الغير على أمر يمنع عنه بتخوف + +56 +00:05:05,540 --> 00:05:10,760 +يعني هو ماكانش هيعمل هذا الأمر لو لا أنه وقع عليه + +57 +00:05:10,760 --> 00:05:17,660 +التخويف و الإجراء على فعل هذا الأمر نكمل التعريف + +58 +00:05:17,660 --> 00:05:25,540 +يقدر الحامل على إيقاعه ويصير الغير خائفا فائط + +59 +00:05:25,540 --> 00:05:31,630 +الرضا المباشرةإذا خد بالك معايا عشان نطلع أركان + +60 +00:05:31,630 --> 00:05:35,550 +الإكراه الأربعة اللي هو الحامل المكره و الفاعل + +61 +00:05:35,550 --> 00:05:41,950 +المكره و المكره عليها و المكره به طبعا أهم شرط من + +62 +00:05:41,950 --> 00:05:47,370 +شروط الإكراه و انه .. خد بالك معايا من التعريف حمل + +63 +00:05:47,370 --> 00:05:51,090 +الغير يعني إجبار الغير على أمر يمتنع عنه بتخويف + +64 +00:05:53,680 --> 00:05:58,280 +أشرب خمرة و لا بضربك و اللي معايا قلم رصاص، ده + +65 +00:05:58,280 --> 00:06:04,740 +أضربك قلم رصاص، هل هذا الدرب يفوت رضاء الإنسان من + +66 +00:06:04,740 --> 00:06:12,820 +مباشرة العمل؟ طيب، واحد حامل برودة و هذه البرودة + +67 +00:06:12,820 --> 00:06:19,660 +أو المسدس لعبةو بقول اشرب خمر و إلا باطخك، هل هذا + +68 +00:06:19,660 --> 00:06:26,840 +يستطيع يعني الفعل؟ لابد أن لا يستطيع هذا الفعل طيب + +69 +00:06:26,840 --> 00:06:30,920 +تمام مرة نرجع، اللي كراه إذن هو إجبار الغير أو + +70 +00:06:30,920 --> 00:06:36,680 +حامل الغير على أمر يمتنع عنه بتخويفيقدر الحامل على + +71 +00:06:36,680 --> 00:06:43,560 +إيقاعه، تمام هذه نقطة، أن الحامل هو المكره، يقدر + +72 +00:06:43,560 --> 00:06:50,280 +على المكره أو على فعل الأمر الذي يريد أن يكره به + +73 +00:06:50,280 --> 00:06:55,730 +الطرف الآخرو يصير الغير فائد الرضا، خلاص، انتهي + +74 +00:06:55,730 --> 00:06:59,590 +الرضا من عنده، و يصير هو عامل إشي مش راضي عنه، و + +75 +00:06:59,590 --> 00:07:06,130 +هذا هو شرط المكره، أن يكون قادرا على يقاع ما خوف + +76 +00:07:06,130 --> 00:07:11,110 +به المكره، من شروط المكره أن يصير خائفا، من أن يقع + +77 +00:07:11,110 --> 00:07:15,490 +به ما يعني أن أنتفى القصد و الإرادة عنده، صير + +78 +00:07:15,490 --> 00:07:20,250 +خايف، خلاص، من شرط المكره عليه أن يفعل المكره و هو + +79 +00:07:20,250 --> 00:07:25,830 +غير راضي بهالإيمان بيكفر واكن قلبه مطمئن بالإيمان + +80 +00:07:25,830 --> 00:07:32,470 +فهو ممتنع عنه لو لا حمله عليه، من شرط المكره به أن + +81 +00:07:32,470 --> 00:07:39,190 +يكون مؤثرا في دفع الفاعل أو المكره إلى تنفيذ ما + +82 +00:07:39,190 --> 00:07:45,840 +أمره به المكرهنأتي الآن لأنواع الإكراه عند العلماء + +83 +00:07:45,840 --> 00:07:52,900 +هذا أمر مهم جدا جدا جدا وهو قضية تقسيم الإكراه عند + +84 +00:07:52,900 --> 00:08:00,240 +الشافعية وعند الأحناف طبعا الإكراه جاء تنويعه عند + +85 +00:08:00,240 --> 00:08:04,680 +العلماء بحسب الأساس الذي تم عليه تقسيم الإكراه + +86 +00:08:07,180 --> 00:08:12,760 +تم تقسيم عليه الإكرار عند الشافعية، يختلف عنه عند + +87 +00:08:12,760 --> 00:08:19,380 +الحانف، الحانفية في كل لهم نظرة في تقسيم الإكرار + +88 +00:08:19,380 --> 00:08:25,470 +إلى أنواعففي الشافعية طبعا بيقسموا الإكراح إلى + +89 +00:08:25,470 --> 00:08:31,490 +قسمين إكراح بحق وإكراح بغير حق أما الحنفية ومن + +90 +00:08:31,490 --> 00:08:37,550 +وافقهم يقسمونه إلى ثلاثة أقسام طبعا في خلاف بينهم + +91 +00:08:37,550 --> 00:08:42,890 +في القسم الثالث من هذه الأقسام بيقسموا طبعا إكراح + +92 +00:08:42,890 --> 00:08:49,050 +ملجئ وإكراح غير ملجئ وما يكون به التهديد بأذىيصيب + +93 +00:08:49,050 --> 00:08:53,230 +أصوله أو فروعه أو أهله، ما يسمى بالإجراء الأدبي + +94 +00:08:53,230 --> 00:08:56,330 +عند البعض، الآن هنشوف هذا الكلام إن شاء الله + +95 +00:08:56,330 --> 00:09:02,210 +بالتفصيل خلينا يعني قبل ما تشوف تفصيل ومعنى + +96 +00:09:02,210 --> 00:09:07,370 +الإجراء الملجئ وغير الملجئ خلينا نشوف ما هو الأساس + +97 +00:09:07,370 --> 00:09:13,030 +الذي قسم عليه علماء الحنفية الإجراء وأيضا الأساس + +98 +00:09:13,030 --> 00:09:17,800 +الذي قسم عليه الشافعية الإجراءأما بالنسبالي + +99 +00:09:17,800 --> 00:09:23,260 +الأحناف كان الأساس في تقسيمهم للإجراء أنهم اعتبروا + +100 +00:09:23,260 --> 00:09:29,460 +المكره به سواء كان قولا أو فعلا طبعا هذا الأصل + +101 +00:09:29,460 --> 00:09:33,860 +تدور عليه الأحكام عند علماء الحنافية أما الأساس + +102 +00:09:33,860 --> 00:09:40,660 +الذيقام عليه تقسيم الإكراه عند الشافعية تبروا طبعا + +103 +00:09:40,660 --> 00:09:44,840 +المكره عليه هناك اعتبروا المكره به و هان المكره + +104 +00:09:44,840 --> 00:09:49,160 +عليه سواء كان قولا أو فعلا وهذا أصل برضه تدور عليه + +105 +00:09:49,160 --> 00:09:55,600 +الأحكام عند الشافعية نيجي للأمر المهم اللي هو + +106 +00:09:55,600 --> 00:09:59,780 +التقسيمات و نشوف معنا كل قسم من هذه الأقسام + +107 +00:09:59,780 --> 00:10:05,000 +الإكراه الملجئأو التام وهو ما يكون بالتهديد باطلاف + +108 +00:10:05,000 --> 00:10:12,200 +النفس أو العضو بحيث يحضن فيه الرضا للمكره ويفسد + +109 +00:10:12,200 --> 00:10:17,380 +فيه اختياره فيصبح المكره في يد المكرهكالقالة + +110 +00:10:17,380 --> 00:10:22,700 +المحضة و ذلك نجيب مثال يعنيه كأن يقول المكره + +111 +00:10:22,700 --> 00:10:27,460 +للمكره إن لم تفعل كذا لأقتلنك أو لأقطعن .. لأقطعن + +112 +00:10:27,460 --> 00:10:31,020 +يدك أو رجلك أو أي عضو من أعضاء جسمه فمثل هذا + +113 +00:10:31,020 --> 00:10:36,140 +التهديد يؤدي إلى انعدام رضا المكره حتى كما يفسد + +114 +00:10:36,140 --> 00:10:41,120 +إيه اختياره وهذا ما يسمى بالإكراه الكامل، الإكراه + +115 +00:10:41,120 --> 00:10:47,400 +التام أو الإكراه الملجبنجي للإكراح اللي غير ملجأ + +116 +00:10:47,400 --> 00:10:51,060 +أو الناقص، طب مين تحكيلنا يلعب سريع؟ احنا بنتكلم + +117 +00:10:51,060 --> 00:10:55,900 +عن تقسيمات الإكراح عند مين؟ أيوة صح عند الشافعية، + +118 +00:10:55,900 --> 00:11:00,760 +في ناس مصحصح معايا، إكراح اللي غير ملجأ أو الناقص، + +119 +00:11:00,760 --> 00:11:04,680 +وهو الذي يعدم فيه الرضا للمكره ولا يفسد اختياره، + +120 +00:11:04,680 --> 00:11:10,270 +هو مش راضي لكنلا يفسد اختياره ولا تنعدم قدرته وهذا + +121 +00:11:10,270 --> 00:11:15,050 +الإكراح يتحقق بالقيد أو الحبس مدة طويلة أو الإكراح + +122 +00:11:15,050 --> 00:11:20,630 +والضرب الذي لا يخاف منه على نفسه التلف لأنه يبقى + +123 +00:11:20,630 --> 00:11:25,290 +اختياره حينئذ ولكن لا يرضى به وهذا النوع من + +124 +00:11:25,290 --> 00:11:30,150 +الإكراح سمناه الإكراح وسمّاه العلماء بالإكراح + +125 +00:11:30,150 --> 00:11:34,530 +الناقص لأن الإنسان يفقد فيه الرضا ولا يفقد + +126 +00:11:34,530 --> 00:11:36,070 +الإختيار + +127 +00:11:37,930 --> 00:11:43,690 +تمام؟ يعني هو مش راضي ينحبس، بس قادر يختار، قادر + +128 +00:11:43,690 --> 00:11:46,830 +يختار يعمل هذا الأمر و يطلع من السجن ولا لأ عشان + +129 +00:11:46,830 --> 00:11:54,430 +هيك سموه إيه إكراه ناقص الإكراه بما يكون به + +130 +00:11:54,430 --> 00:11:58,390 +التهديد بأذى يصيب الأصول أو الفروع من أهله وهذا + +131 +00:11:58,390 --> 00:12:04,850 +اللي سموه العلماء اللي هو الإكراه الأدبيطبعا فيه + +132 +00:12:04,850 --> 00:12:10,050 +لا يعدى فيه رضا لإنه مش واقع عليه هو الإكراه واقع + +133 +00:12:10,050 --> 00:12:14,050 +على أصل من أصوله ولد من ولاده، بنت من بناته أو + +134 +00:12:14,050 --> 00:12:21,630 +أقصد أبوه وامه اللي هي أصوله وفروعه أحد أبنهم طبعا + +135 +00:12:21,630 --> 00:12:29,020 +إلا أن هذا الإكراه يكون المكره بحبس أبيهأو ابنه أو + +136 +00:12:29,020 --> 00:12:33,500 +زوجته أو أخته، هذا النوع محل يعني خلاف بين علماء + +137 +00:12:33,500 --> 00:12:39,500 +الهدفين نيجي لتقسيم الإكراف، طبعا عند الشافعية + +138 +00:12:39,500 --> 00:12:46,300 +الإكراف بحق وإكراف بغير حق، وهو ما يكون على أمر + +139 +00:12:46,300 --> 00:12:52,560 +واجب شرعانويصح الإقدام عليه، وهذا القسم لا تنقطع + +140 +00:12:52,560 --> 00:12:57,580 +فيه نسبة الفعل عن الفاعل، فيصح بيع المديون القادر + +141 +00:12:57,580 --> 00:13:03,240 +لماله وفاء لدينه، ويصح طلاق المولى من زوجته بعد + +142 +00:13:03,240 --> 00:13:10,610 +انقضاء مدة الإهلاءأحنا يعني خلّينا نقول أن القسم + +143 +00:13:10,610 --> 00:13:16,270 +الأول أن هناك حقًا الذي بقف عليه علماء الشفعية + +144 +00:13:16,270 --> 00:13:20,230 +اللي هو الإقرار بحق أن هناك حقًا وهذا الحق يعترف + +145 +00:13:20,230 --> 00:13:28,590 +به المشرعهذا المديون مماطل و عنده مال، فبيحجزوا + +146 +00:13:28,590 --> 00:13:33,330 +على ماله، ببيعوه عشان يوفوا هذا الدين، هذا بيسموه + +147 +00:13:33,330 --> 00:13:37,910 +إكراه بحق أما الإكراه بغير حق، وهو ما لا يصح + +148 +00:13:37,910 --> 00:13:42,970 +الإقدام عليه، وهو الإكراه على أمر منهي، عنده شرعا + +149 +00:13:42,970 --> 00:13:49,120 +كإكراه على القتل أو الزنا أو شرب الخمرالان نجي + +150 +00:13:49,120 --> 00:13:53,220 +لأثر الإكراح على الأهلية، الإكراح طبعا لا ينافي + +151 +00:13:53,220 --> 00:13:58,520 +الأهلية لأهلية الوجوب ولا أهلية الأداء وذلك لكمال + +152 +00:13:58,520 --> 00:14:02,560 +العقل والبدن وعدم الإخلال بهم بالإكراح كما أن + +153 +00:14:02,560 --> 00:14:06,780 +الإكراح لا يوجب سقوط الخطاب على المجرح بأي حالة من + +154 +00:14:06,780 --> 00:14:12,700 +الأحوال سواء كان ملجئ أو غير ملجئ نجي لأثر الإكراح + +155 +00:14:12,700 --> 00:14:17,980 +في الأقوال والأفعالطبعا كل ما يصدر عن المكره إما + +156 +00:14:17,980 --> 00:14:23,720 +أن يكون قولا أو فعلا، خدنا نشوف القول أول الأقوال + +157 +00:14:23,720 --> 00:14:29,960 +هذه منها ما يقبل الفسخ ومنها ما لا يقبل الفسخما لا + +158 +00:14:29,960 --> 00:14:33,180 +يقبل فسخ من الأقوال إذا كان المكره عليه قولا غير + +159 +00:14:33,180 --> 00:14:37,460 +قابل للفسخ ولا يتوقف على الرضا، زي الطلاق، الزواج + +160 +00:14:37,460 --> 00:14:43,340 +و العدو، فإن حكمه لا يبطل بالإجراء وينفذ إذ لا + +161 +00:14:43,340 --> 00:14:48,580 +تأثير للإجراء عليه، فإن هذه التصرفات لا تحتمل فسخ + +162 +00:14:48,580 --> 00:14:52,660 +تتوقف على الإختيار دون الرضا، حتى لو طلقة أو تزوج + +163 +00:14:52,660 --> 00:14:59,000 +أو أعتقى بالإجراء تصح لأنها لا تبطل بالهزلوهذا هو + +164 +00:14:59,000 --> 00:15:02,660 +قول الأحناف خلف في الجمهور الذين رأوا عدم تحميل + +165 +00:15:02,660 --> 00:15:06,820 +القائل تبع قول لم يتلفظ به راضيا مختارا بل فرض + +166 +00:15:06,820 --> 00:15:15,280 +عليه بالقوة ولا يجد حيلة في دفعهوالراجح من القولين + +167 +00:15:15,280 --> 00:15:20,680 +هو قول الجمهور نقول بعدم تحميل القائل وهو طبعا هذا + +168 +00:15:20,680 --> 00:15:27,380 +الراجح تبعت قول صدرة منه دون رغبة أو اختيار لأننا + +169 +00:15:27,380 --> 00:15:30,980 +وجدنا فيه النصوص الشرعية وأقول الصحابة والتابعين + +170 +00:15:30,980 --> 00:15:32,980 +ما يؤيد ذلك + +171 +00:15:35,800 --> 00:15:45,440 +يعني الآن الأحناف بدهم يحملوا المكرع نتيجة ما أكره + +172 +00:15:45,440 --> 00:15:50,540 +عليه لكن جمهور العلماء رفضوا تحميل القائل تبعت قول + +173 +00:15:50,540 --> 00:15:55,020 +لم يتلفظ به وهو راد المختار وخصوصا قضية الطلاق + +174 +00:15:55,020 --> 00:16:03,150 +وقضية أزواج و لا عدقما يقبل الفسخ من الأقوال إذا + +175 +00:16:03,150 --> 00:16:07,450 +كان المكره عليه قولا قابلا للفسخ ويتوقف على الرضا، + +176 +00:16:07,450 --> 00:16:10,910 +هنا فرق الأحناف بين هذا القسم وسابقه، وقالوا أن + +177 +00:16:10,910 --> 00:16:15,190 +التصرفات التي تقبل الفسخ تتوقف على الرضا، لا تعتبر + +178 +00:16:15,190 --> 00:16:20,470 +صحيحة مع الإجراء، بعقد البيع وغيره من العقود التي + +179 +00:16:20,470 --> 00:16:27,200 +تقبل الفسخ والإجراء يفسدهافينعقد البيع فاسدا وقول + +180 +00:16:27,200 --> 00:16:31,460 +المكره لاغيا لأن هذا كلام من؟ كلام الأحناف أما + +181 +00:16:31,460 --> 00:16:35,980 +كلام الجمهور لما يفرقوا بين هذا القسم وسابقه فإن + +182 +00:16:35,980 --> 00:16:41,200 +جميع التصرفات الواقعة تحت تأثير الإكراه باطلة سواء + +183 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اتفقنا سابقا أن كل عارض من + +195 +00:17:48,390 --> 00:17:55,440 +العوارض تعريفهوأثروا على الأهلية أو الأحكام اللى + +196 +00:17:55,440 --> 00:18:00,660 +بتتعلق فيه بالاهلية نسأل الله سبحانه و تعالى لكم + +197 +00:18:00,660 --> 00:18:06,340 +التوفيق والسداد والنجاح والسلام عليكم ورحمة الله + +198 +00:18:06,340 --> 00:18:07,460 +وبركاته + diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/BJLKf__lPPc_raw.json b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/BJLKf__lPPc_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..02c3362a3b168e524f26089bde899d23cd111412 --- /dev/null +++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/BJLKf__lPPc_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 3322, "start": 6.06, "end": 33.22, "text": "أعوذ بالله من الشيطان الرجيم بسم الله الرحمن الرحيم أسعد الله أوقاتكم بالخير واليمني والسعادة بناتي الطيبات ربنا يبارك فيكم وفي أوقاتكم أهلا وسهلا بكم جميعا في لقائنا المتجدد دوما سنكون اليوم مع موضوع مهم من مواضيع علم أصول الفقه وهو الصياغ التي تعرف بها 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00:00:15,230 +الحمد لله الواحد الأحد الفرد الصمد لم يلد ولم يولد + +3 +00:00:15,230 --> 00:00:18,370 +ولم يكن له كفوا أحد، وصلاة وسلام على خير الأنام + +4 +00:00:18,370 --> 00:00:22,230 +محمد وعلى آله وصحبه أجمعين، وبعد بداية أخواتي + +5 +00:00:22,230 --> 00:00:25,830 +الحبيبات وبنات الطيبات، أحيكم بتحية الإسلام العظيم + +6 +00:00:25,830 --> 00:00:29,410 +السلام عليكم ورحمة الله وبركاته، اليوم إن شاء الله + +7 +00:00:29,410 --> 00:00:37,080 +هنكون مع آخر ما يتعلق بمساق أصول الفقه واحد، وكنا قد + +8 +00:00:37,080 --> 00:00:42,140 +توقفنا مع المصدر الثالث من مصادر التشريع، والمجمع + +9 +00:00:42,140 --> 00:00:47,960 +عليها طبعًا وهو الإجماع، وظل عندنا القياس، أما + +10 +00:00:47,960 --> 00:00:52,540 +بالنسبة لي الإجماع يعني هنمشي زي ما مشينا في القرآن + +11 +00:00:52,540 --> 00:00:57,100 +والسنة نفس الحاجة، هنمشي في الإجماع والقياس اللي هو + +12 +00:00:57,100 --> 00:01:01,580 +التعريف، معرفة ما يتعلق به، ما من أحكام بأول عجلة + +13 +00:01:01,580 --> 00:01:06,100 +هنتعرف على معنى الإجماع في اللغة، والإصطلاح في + +14 +00:01:06,100 --> 00:01:11,200 +اللغة يطلق عليه معنيين، المعنى الأول العزم على الشيء + +15 +00:01:11,200 --> 00:01:15,840 +والتصميم عليه، يقال أجمع فلان على الأمر أي عزم + +16 +00:01:15,840 --> 00:01:20,520 +عليه، ومنه قول الرسول صلى الله عليه وسلم: "صيامُ من لم يُجمع صيامُه من الليل" أي يعزم أو ينوي، أما المعنى + +17 +00:01:20,520 --> 00:01:25,830 +الثاني في اللغة الاتفاق، يقال أجمع القوم على كذا أي + +18 +00:01:25,830 --> 00:01:32,150 +اتفقوا عليه، يعني المعنى الأول العزم، والثاني + +19 +00:01:32,150 --> 00:01:35,710 +الاتفاق، والفرق بين المعنى الأول والثاني أن الأول + +20 +00:01:35,710 --> 00:01:38,750 +يطلق على عزم الواحد، نفر الإنسان بذاته يعني، والثاني + +21 +00:01:38,750 --> 00:01:43,510 +لابد فيه من متعدد، أما الإجماع في الإصطلاح هو اتفاق + +22 +00:01:43,510 --> 00:01:50,380 +المجتهدين من أمة محمد صلى الله عليه وسلم بعد + +23 +00:01:50,380 --> 00:01:53,280 +وفاته في أي عصر من العصور على حكم شرعي، يعني لو + +24 +00:01:53,280 --> 00:01:58,320 +جبت لك العبارة هذه في صح و غلط، وكتبت لك الإجماع + +25 +00:01:58,320 --> 00:02:02,240 +هو اتفاق المجتهدين من أمة محمد صلى الله عليه وسلم + +26 +00:02:02,240 --> 00:02:06,180 +قبل وفاته في عصر من العصور على حكم شرعي، أيوة يا + +27 +00:02:06,180 --> 00:02:14,470 +أسماء كلامك مظبوط، خطأ، العبارة هيك بتكون لأن + +28 +00:02:14,470 --> 00:02:18,690 +الإجماع لا يكون إلا بعد وفاة الرسول صلى الله عليه + +29 +00:02:18,690 --> 00:02:23,470 +وسلم، أما في حياته ما فيش إجماع، لأنه المشرع موجود + +30 +00:02:23,470 --> 00:02:26,570 +وعندنا تعريف آخر للإجماع طبعًا، أنت عندك الحرية تحفظ + +31 +00:02:27,690 --> 00:02:32,370 +التعريف الأول أو الثاني، هو اتفاق المجتهدين من + +32 +00:02:32,370 --> 00:02:36,350 +المسلمين في أي عصر من العصور بعد وفاة الرسول صلى + +33 +00:02:36,350 --> 00:02:40,310 +الله عليه وسلم على حكم شرعي في قضية لم يرد بحكمها + +34 +00:02:40,310 --> 00:02:44,870 +نص، أيضًا خلينا نتكلم عن أركان الإجماع، عندنا أول + +35 +00:02:44,870 --> 00:02:52,260 +ركن صدور الاتفاق، معناه أن يكون رأي كل واحد وفق + +36 +00:02:52,260 --> 00:02:56,740 +الآخر، يعني كلهم متفقين على رأي واحد، وصدورها دا + +37 +00:02:56,740 --> 00:03:01,670 +من المجتهدين، إذ لا عبرة بوجود غيرهم، يعني ما ينفعش + +38 +00:03:01,670 --> 00:03:05,250 +غير أهل الاجتهاد يكونوا هم اللي بدهم يتفقوا على + +39 +00:03:05,250 --> 00:03:11,270 +أمر شرعي معين، والمجتهد هو الذي حصلت له ملكة يقدر + +40 +00:03:11,270 --> 00:03:16,330 +بيها على استنباط الأحكام من مصادرها، ويُشترط في + +41 +00:03:16,330 --> 00:03:20,270 +المجتهد عدة شروط، العلم بالقرآن الكريم والسنة + +42 +00:03:20,270 --> 00:03:23,450 +ومسائل الاجتهاد، أن يكون عالمًا بأصوله، أن يكون ملمًا + +43 +00:03:23,450 --> 00:03:27,230 +باللغة العربية وغيرها، من الشرط أن يكون المجمعون من + +44 +00:03:27,230 --> 00:03:31,970 +أمة محمد صلى الله عليه وسلم، يُشترط أن يكون أهل + +45 +00:03:31,970 --> 00:03:37,610 +الإجماع الذي نعنيه من المسلمين، وهو كل من أجاب دعوة + +46 +00:03:37,610 --> 00:03:42,690 +رسول الله صلى الله عليه وسلم، وأمن بما جاء به، وقد + +47 +00:03:42,690 --> 00:03:47,270 +اتفق العلماء أنه لا عبرة بالكافر في هذا الموضوع + +48 +00:03:47,270 --> 00:03:50,430 +ولا عبرة أيضًا باتفاق الأمم السابقة، أيضًا اتفاق + +49 +00:03:50,430 --> 00:03:56,010 +المجتهدين بعد وفاة الرسول صلى الله عليه وسلم لا + +50 +00:03:56,010 --> 00:03:59,130 +عبرة في الاتفاق الحاصل في عصره، لأنه وفق قول + +51 +00:03:59,130 --> 00:04:03,150 +المجتمعين قول النبي صلى الله عليه وسلم، فالحجة هي + +52 +00:04:03,150 --> 00:04:07,510 +قول النبي وليست أقوال المجمعين، بمعنى إن الواقعة + +53 +00:04:07,510 --> 00:04:13,020 +التي يحصل الإجماع عليها يجب أن تكون بعد وفاة + +54 +00:04:13,020 --> 00:04:18,980 +النبي صلى الله عليه وسلم، بحيث لم يرد فيها نص من + +55 +00:04:18,980 --> 00:04:23,480 +القرآن أو سنة، اتفاق المجتهدين في عصر من العصور + +56 +00:04:23,480 --> 00:04:27,900 +والمقصود هنا اتفاق من هم أهل الاتفاق اللي هم + +57 +00:04:27,900 --> 00:04:31,180 +العلماء المجتهدين، والمراد بالعصر هو عصر من كان من + +58 +00:04:31,180 --> 00:04:36,480 +أهل الاجتهاد في الوقت الذي حدثت فيه المسائل + +59 +00:04:36,480 --> 00:04:39,760 +الجديدة التي تتطلب حكمًا شرعيًا، الاتفاق على حكم شرعي + +60 +00:04:39,760 --> 00:04:45,530 +فالمقصود بالإجماع اللي لديه يعنيه هنا هو ما يختص + +61 +00:04:45,530 --> 00:04:48,850 +بالحكم الشرعي لحل البيع وحرمة الخمر، وليس ما يتعلق + +62 +00:04:48,850 --> 00:04:53,010 +باللغة والعلوم وقراء الناس في الحال، أما عن أنواع + +63 +00:04:53,010 --> 00:04:58,870 +الإجماع عندنا إجماع صريح وإجماع سكوتي، أما الإجماع + +64 +00:04:58,870 --> 00:05:03,930 +الصريح هو ما اتفق فيه جميع المجتهدين أي عصر على حكم + +65 +00:05:03,930 --> 00:05:09,410 +واقعة بإبداء كل واحد منهم رأيه صراحة بفتوى أو قضاء + +66 +00:05:09,410 --> 00:05:15,060 +وهذا النوع من الإجماع أداة قاطعة لا يحكم في واقعة + +67 +00:05:15,060 --> 00:05:19,960 +في واقعة بخلافه، ويجب العمل به، مثل الإجماع على حد + +68 +00:05:19,960 --> 00:05:25,940 +شارب الخمر، 80 جدعان، عندنا الإجماع السكوتي وهو + +69 +00:05:25,940 --> 00:05:31,370 +التسمية الثاني، وهو ما يراه بعض المشتغلين في عصر حول + +70 +00:05:31,370 --> 00:05:35,010 +حادثة أو واقعة معينة بفتوى أو قضاء، فيسكت الآخرون + +71 +00:05:35,010 --> 00:05:39,830 +فيسكت الآخرون دون عذر يمنعهم من الموافقة أو + +72 +00:05:39,830 --> 00:05:43,490 +المخالفة، تمام؟ زي ما بيقول السكوت علامة الرضا، وهذا + +73 +00:05:43,490 --> 00:05:47,290 +النوع إن الإجماع مختلف فيه لعدم الجزم بموافقة + +74 +00:05:47,290 --> 00:05:50,970 +الساكت، مثل الإجماع على تحريم بعض المطعمات قبل + +75 +00:05:50,970 --> 00:05:55,650 +قبضها، أما بالنسبة لمرتبة الإجماع وحجيته يأتي + +76 +00:05:55,650 --> 00:06:01,330 +الإجماع في المرتبة الثالثة من بين مصادر التشريع + +77 +00:06:01,330 --> 00:06:04,370 +الإسلامي، طيب إيش المرتبة الأولى؟ أه صح القرآن + +78 +00:06:04,370 --> 00:06:07,730 +الكريم، والثانية السنة النبوية، والثالثة اللي هي + +79 +00:06:07,730 --> 00:06:12,030 +الإجماع، إذا توفرت فيه الشروط المطلوبة شرحًا، أنه يثبت + +80 +00:06:12,030 --> 00:06:17,150 +المراد به على سبيل اليقين بأن يكون موجبًا للحكم + +81 +00:06:17,150 --> 00:06:21,330 +قاطعًا كالكتاب والسنة، وهذا هو قول عامة المسلمين + +82 +00:06:21,330 --> 00:06:26,090 +وجمهور العلماء، بقوله تعالى: ﴿وكذلك جعلناكم أمة وسطًا﴾ + +83 +00:06:26,090 --> 00:06:31,530 +لتكونوا شهداء على الناس، ودليل ذلك أيضًا سنة المصطفى + +84 +00:06:31,530 --> 00:06:37,070 +في قوله: "لا تجتمع أمتي على الخطأ"، وفي رواية "على + +85 +00:06:37,070 --> 00:06:40,830 +الضلالة"، نأتي الآن بالحديث عن المرتبة أو الدليل + +86 +00:06:40,830 --> 00:06:51,910 +الرابع أو المصدر الرابع من المصادر الأصلية المتفق + +87 +00:06:51,910 --> 00:06:56,950 +عليها وهو القياس، وهو آخر مصدر، يعني المصادر اللي + +88 +00:06:56,950 --> 00:07:01,730 +بيطلعوا عنها أربعة: قرآن وسنة وإجماع، والمصدر الأخير + +89 +00:07:01,730 --> 00:07:06,790 +في المصادر المتفق عليها القياس، القياس طبعًا معناه في + +90 +00:07:06,790 --> 00:07:13,450 +اللغة وفي الإصطلاح، القياس في اللغة تقدير شيء على + +91 +00:07:13,450 --> 00:07:17,930 +مثال شيء آخر وتسويته به، ولذلك سمي الميكانيكي قياسًا + +92 +00:07:17,930 --> 00:07:24,710 +ويقال: فلان لا يقاس بفلان، أي لا يساوي، وقيل هو + +93 +00:07:25,250 --> 00:07:29,950 +مصدر قسط الشاي، إذا اعتبرته أقيسه قيسًا وقياسًا، ومن + +94 +00:07:29,950 --> 00:07:35,530 +ذلك اتضح لنا أن القياس له استعمالات ثلاثة في اللغة + +95 +00:07:35,530 --> 00:07:38,450 +الأول للتقدير الحسي، والثاني للتسوية، والثالث + +96 +00:07:38,450 --> 00:07:43,230 +للاعتبار والنظر، للاعتبار والنظر، أما الأول للتقدير + +97 +00:07:43,230 --> 00:07:48,690 +الحسي، يقول: قستُ ثوبًا بالذراع، إذا قدرته بإيه، وقست + +98 +00:07:48,690 --> 00:07:53,870 +الأرض بالمتر، هذا تقدره بسميه حسي، أما للتسوية، + +99 +00:07:53,870 --> 00:07:59,350 +المعنى الثاني، وهو مفهوم معنوي على أغلب استعمالاته + +100 +00:07:59,350 --> 00:08:03,010 +كما يقول: فلان يسوي فلانًا فضلًا وأشرفًا ومكانة، أما + +101 +00:08:03,010 --> 00:08:08,490 +الاستعمال الثالث، ده باعتبار ونظر، باعتبار يا + +102 +00:08:08,490 --> 00:08:13,790 +أولي الأبصار بمعنى: قيسوا حالكم على حالهم، أما في + +103 +00:08:13,790 --> 00:08:20,670 +الإصطلاح الحق أمر غير منصوص عليه حكمه بأمر منصوص على + +104 +00:08:20,670 --> 00:08:27,290 +حكم اشتراكهما في علّة الحكم، كمان تعريف عندنا، وزي + +105 +00:08:27,290 --> 00:08:32,610 +ما حكينا إذا تعددت التعريفات احفظيه تعريف واحد + +106 +00:08:32,610 --> 00:08:35,870 +فقط، الحق واقع لم يرد نص شرعي على حكمها بواقع ورد + +107 +00:08:35,870 --> 00:08:40,990 +نص شرعي بحكمها لإشتراك الواقعتين في علّة هذا الحكم + +108 +00:08:40,990 --> 00:08:47,030 +أما بالنسبة لأركان القياس أربعة: أصل وفرع وحكم الأصل وعلّة + +109 +00:08:47,030 --> 00:08:51,310 +الأصل، ولا إله إلا الله، طبعًا الأصل وهو ما ورد بحكمه نص + +110 +00:08:51,310 --> 00:08:56,500 +ويسمى أيضًا المقيس عليه، الفرع هو الشيء الذي لم يرد + +111 +00:08:56,500 --> 00:09:00,500 +بحكمه نص، ويراد تسويته بالأصل في حكمه، ويسمى + +112 +00:09:00,500 --> 00:09:04,980 +بالمقيس له، حكم الأصل هو الحكم الشرعي الذي ورد به نص + +113 +00:09:04,980 --> 00:09:10,500 +في الأصل، ويراد أن يكون مثله في الفرع، أما العلة وهي + +114 +00:09:10,500 --> 00:09:16,410 +الوصف أو السبب الذي بني عليه حكم الأصل، وجوده في + +115 +00:09:16,410 --> 00:09:22,210 +الفرع، هذه الأركان الأربعة للقياس، كثير هنا أمثلة + +116 +00:09:22,210 --> 00:09:27,630 +للقياس، خلينا نشوف بعض منها، قول النبي صلى الله عليه + +117 +00:09:27,630 --> 00:09:31,310 +وسلم: "ليرث القاتل القتل"، يمنع الميراث إذا حصلها من + +118 +00:09:31,310 --> 00:09:37,110 +الوارث، العلة في ذلك هو استعجال الشيء قبل أوانه + +119 +00:09:37,110 --> 00:09:40,650 +فيعاقب بحرمانه، قيس عليه الموظف له إذا قتل الموظف، + +120 +00:09:40,650 --> 00:09:46,330 +فالأصل حكمه منع الميراث، ولما تحققت نفس العلة + +121 +00:09:46,330 --> 00:09:50,070 +المذكورة في الفرع، الموظف له أخذ نفس الحكم، نيجي + +122 +00:09:50,070 --> 00:09:57,370 +لأقسام القياس وأنواعه، بالنسبة للقياس كما قلنا هو + +123 +00:09:57,370 --> 00:10:05,470 +اشتراك الفرع مع الأصل فيه العلة، إلا أن العلة قد + +124 +00:10:05,470 --> 00:10:09,810 +تكون في الفرع أقوى منها في الأصل، بنسميها ده قياس + +126 +00:10:13,530 --> 00:10:19,490 +الأولى وقد تكون في الفرع مساوية لما فيه الأصل وهو + +127 +00:10:19,490 --> 00:10:23,810 +القياس المساوي وقد تكون في الفرع أضعف منها في + +128 +00:10:23,810 --> 00:10:29,090 +الأصل وهو قياس الأدنى يعني بيكون عندنا تلت أنواع + +129 +00:10:29,090 --> 00:10:35,130 +قياس الأولى والقياس المساوي والقياس الأدنى خلّينا + +130 +00:10:35,130 --> 00:10:40,130 +نشوف كل نوع من هذه الأنواع ونفصل الحديث فيه + +131 +00:10:40,130 --> 00:10:44,630 +والقياس الأولى هو ما كانت علّة الفرع أقوى منها في + +132 +00:10:44,630 --> 00:10:48,790 +علّة الأصل فيكون ثبوت الحكم للفرع أولى من ثبوته + +133 +00:10:48,790 --> 00:10:55,090 +للأصل بطريقة الأولى ومعنى الكلام هذا خلّينا نشوفه + +134 +00:10:55,090 --> 00:11:00,000 +من خلال المثال تحريم الضرب قياسا على التأفيف + +135 +00:11:00,000 --> 00:11:04,960 +والعلّة الجامعة هي ذا تمام؟ وذلك في قوله تعالى + +136 +00:11:04,960 --> 00:11:09,960 +فلا تقول لهم أف فالعلّة موجودة في ضرب الوالدين + +137 +00:11:09,960 --> 00:11:14,560 +بشكل أقوى وأشد مما في الأصل فيكون تحريم ضرب + +138 +00:11:14,560 --> 00:11:19,280 +الوالدين بالقياس على موضع النص بطريق قياس الأولى + +139 +00:11:19,280 --> 00:11:25,360 +يعني هو يحرم الاعتداء على الوالدين بالأف فما بالك + +140 +00:11:25,360 --> 00:11:30,590 +بالضرب؟ إذاً هذا بنسميه قياس الأولى إذا وضرب أولى + +141 +00:11:30,590 --> 00:11:36,350 +من كلمة أف فيكون أيضًا محرمة قياسًا على قوله تعالى + +142 +00:11:36,350 --> 00:11:41,330 +فلا تقولهم أف أما النوع الثاني وهو القياس المساوي + +143 +00:11:41,330 --> 00:11:45,070 +وهو ما كانت العلة التي بني عليها الحكم في الأصل + +144 +00:11:45,070 --> 00:11:49,250 +موجودة في الفرع بقدر ما هي متحققة أيضًا في الأصل + +145 +00:11:49,250 --> 00:11:53,750 +زي إيش زي قياس حرمة أكل مال اليتيم ظلمًا على + +146 +00:11:53,750 --> 00:11:57,920 +إحراقه كما في قوله تعالى إن الذين يأكلون أموال + +147 +00:11:57,920 --> 00:12:01,740 +اليتامة ظلمًا إنما يأكلون في بطونهم نارًا فعلّة + +148 +00:12:01,740 --> 00:12:05,200 +الحكم هي الاعتداء على مال اليتيم واتلافه عليه + +149 +00:12:05,200 --> 00:12:10,180 +وإحراق مال اليتيم يساوي واقعة النص في الحرمة فيكون + +150 +00:12:10,180 --> 00:12:14,600 +حكمه حكم أكله ظلمًا أي تحريمه يعني أكل مال اليتيم + +151 +00:12:14,600 --> 00:12:18,180 +وإحراق مال اليتيم واحد ما هو الإحراق نفس الأكل كله + +152 +00:12:18,180 --> 00:12:25,750 +اتلافه أما القياس الأدنى قوله تعالى فاعتبروا يا + +153 +00:12:25,750 --> 00:12:31,430 +أولي الأبصار و أما السنة بحديث معاذ رضي الله عنه + +154 +00:12:31,430 --> 00:12:36,530 +المعروف لما أرسله الرسول صلى الله عليه و سلم قاضيا + +155 +00:12:36,530 --> 00:12:44,370 +إلى اليمن هنا خلّينا نقول إن هذه المصادر الأربع + +156 +00:12:44,370 --> 00:12:47,910 +اللي اتحدثنا عنها القرآن الكريم والسنة النبوية + +157 +00:12:47,910 --> 00:12:52,190 +والإجماع والقياس هذه المصادر الأربع هي المصادر + +158 +00:12:52,190 --> 00:12:57,230 +المتفق عليها بين علماء الجمهور طيب في حد هنا بيسأل + +159 +00:12:57,230 --> 00:13:01,510 +إن في مصادر غير هذه المصادر؟ آه أحنا الآن المصادر + +160 +00:13:01,510 --> 00:13:05,830 +الأصلية اللي هي الأربعة دول المتفق عليها عند + +161 +00:13:05,830 --> 00:13:10,950 +العلماء قرآن، سنة، 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+00:00:22,290 --> 00:00:26,350 +بداية أخوات الحبيبات وبنات الطيبات وحيكم بتحية + +6 +00:00:26,350 --> 00:00:30,510 +طيبة مباركة من عند الله السلام عليكم ورحمة الله + +7 +00:00:30,510 --> 00:00:34,130 +وبركاته اللهم علمنا ماينفعنا وانفعنا بما علمتنا + +8 +00:00:34,130 --> 00:00:38,090 +وازدنا علما اللهم آميناليوم إن شاء الله هتكلم + +9 +00:00:38,090 --> 00:00:43,970 +معاكم في هذه المحاضرة عن التكليف وكيف تعمل التكليف + +10 +00:00:43,970 --> 00:00:49,270 +المطلوب منك طبعاً في صفحة الـ Model عندك بالنسبة + +11 +00:00:49,270 --> 00:00:55,570 +لطالبات أصول الفقه المصادر الأصلية تدخلي على صفحتك + +12 +00:00:55,570 --> 00:00:59,750 +في الأيقونات اللي موجودة عندك زي ما أنت شايفها + +13 +00:00:59,750 --> 00:01:08,510 +معايا هتلاقي حاجة عندك هنا اسمهانموذج لبطاقة كتاب + +14 +00:01:08,510 --> 00:01:15,190 +كمثال على التكليف نموذج لبطاقة كتاب كمثال على + +15 +00:01:15,190 --> 00:01:23,310 +التكليف طبعا التكليف خلنا نقول عبارة عن بطاقة كتاب + +16 +00:01:23,310 --> 00:01:28,270 +أو بدنا نسميه بطاقة كتاب البطاقة هذه إيش بدى أكتب + +17 +00:01:28,270 --> 00:01:32,530 +فيها؟ زي مثلا بطاقة الهوية إيش بدى يكون مكتوب في + +18 +00:01:32,530 --> 00:01:38,130 +بطاقة الهوية؟ إسمك و سنكوعنوانك البيانات تبعتك + +19 +00:01:38,130 --> 00:01:42,810 +يعني الآن أنا في بطاقة الكتاب بدي البيانات اللي + +20 +00:01:42,810 --> 00:01:49,050 +بتتعلق بهذا الكتاب محتاجة بس البيانات اللي موجودة + +21 +00:01:49,050 --> 00:01:54,410 +على بطاقة الكتاب الخارجية مش محتاجة يعني افتح + +22 +00:01:54,410 --> 00:02:00,050 +الكتاب واخد منه من جوا معلومات فقط محتاجة بس + +23 +00:02:00,050 --> 00:02:07,600 +البطاقة التعريفية لهذا الكتاب مثلا عندىكتابة اسمه + +24 +00:02:07,600 --> 00:02:13,540 +أصول السراخسية تمام؟ أصول السراخسية على طول هنا + +25 +00:02:13,540 --> 00:02:21,820 +طلعت الكتاب هي زي ما انتوا شايفين اسم الكتاب تحته + +26 +00:02:21,820 --> 00:02:30,440 +على طول اسم مؤلف الكتاب زي ما احنا شايفين أحمد بن + +27 +00:02:30,440 --> 00:02:38,330 +أبي سهل السراخسيفيه هندق هذا الكتاب عبارة عن + +28 +00:02:38,330 --> 00:02:42,830 +مجلدين أو معبارة عن جزئين اللي قدامي الجزء الأول + +29 +00:02:42,830 --> 00:02:48,550 +بروح أنا كاتبة عدد أجزاء الكتاب جزئين بشوف دار + +30 +00:02:48,550 --> 00:02:53,570 +النشر أو رقم الطبعة أو سنت الطبعة حسب ما هو موجود + +31 +00:02:53,570 --> 00:03:02,330 +هاي كل اللي مطلوب بنزل تحت في عند ايقونةمكتوب فيها + +32 +00:03:02,330 --> 00:03:07,810 +كتب أصول الفقه للمساعدة في عمل التكليف موجود عندك + +33 +00:03:07,810 --> 00:03:14,230 +هنا، هاي ملف، تضغط عليه هذا الملف، هاي هنا، تنزيل + +34 +00:03:14,230 --> 00:03:20,390 +الملف، هذا ملف اكسل هذا الملف في كل الكتب، هاي نزل + +35 +00:03:20,390 --> 00:03:28,490 +عندي أنا تمام، + +36 +00:03:28,490 --> 00:03:34,530 +هذا الملف مكتوب فيكل أسامي الكتب اللي موجودين في + +37 +00:03:34,530 --> 00:03:39,550 +الجامعة الإسلامية، في مكتبة الجامعة الإسلامية + +38 +00:03:39,550 --> 00:03:47,490 +تمام؟ هو شكله هايهم، هفتحلكم الملف زي ما أنتوا + +39 +00:03:47,490 --> 00:03:52,470 +شايفين، هاي كل الكتب اللي موجودين في مكتبة الجامعة + +40 +00:03:52,470 --> 00:03:58,210 +ماعليك إلا تختاري اسم كتاب من هدول الكتبوتروح تاخد + +41 +00:03:58,210 --> 00:04:03,830 +اسم المؤلف تبعه وانت عاد تدخلي على ال net أو انت + +42 +00:04:03,830 --> 00:04:08,170 +بتاخد تستكمل باقى البيانات من الملف هاي في عندي + +43 +00:04:08,170 --> 00:04:14,370 +هان سنة الطبع وفي عندي مكان الطبع وإن هان موجود + +44 +00:04:14,370 --> 00:04:18,270 +عندى في الجامعة في إشي في المكتبة المركزية وفي إشي + +45 +00:04:18,270 --> 00:04:25,490 +موجودفي مكتبة الجنوب في اشي موجود في مكتبة المراجع + +46 +00:04:25,490 --> 00:04:31,350 +تمام؟ اذا هذا التكليف زي ما حكينا بتستعيني في + +47 +00:04:31,350 --> 00:04:35,250 +تستعيني في عمل التكليف هذا من خلال الكتب اللي + +48 +00:04:35,250 --> 00:04:40,730 +موجودين عندك في ملف اكسل بتختار كتاب و بتعمله الآن + +49 +00:04:40,730 --> 00:04:46,310 +هتلاقي في عندك ايقونة وهذا الايقونة موجود او مكتوب + +50 +00:04:46,310 --> 00:04:55,660 +عليهاتسليم التكليف، مكتوب عليها هي تسليم التكليف، + +51 +00:04:55,660 --> 00:05:00,320 +هي عندي هذه الـ Icona مكتوب عليها تسليم التكليف، + +52 +00:05:00,320 --> 00:05:06,760 +بتروح ضغط على المجلد اللي موجود فيه تسليم التكليفو + +53 +00:05:06,760 --> 00:05:12,120 +بتروحي محملة الملف تبعك اما على word أو عبدي اف و + +54 +00:05:12,120 --> 00:05:16,660 +بكون هيك انت سلمتيني التكليف بيوصلني انك سلمتي + +55 +00:05:16,660 --> 00:05:21,540 +التكليف طبعا هيكون موعد تسليم التكليف هيبدأ من + +56 +00:05:21,540 --> 00:05:26,940 +اليوم ان شاء الله لغاية انتهاء الامتحانات النصفية + +57 +00:05:27,510 --> 00:05:31,790 +هديكي فرصة كافية انك تعملي هذا التكليف طبعا + +58 +00:05:31,790 --> 00:05:36,190 +بتكتبيه على word بتخزنيه و بتحمليه على الملف اللي + +59 +00:05:36,190 --> 00:05:41,030 +موجود فيه هنا تسليم التكليف هذا كل ما يتعلق + +60 +00:05:41,030 --> 00:05:48,830 +بالتكليف سهل و بسيط و ميسور على أساس انه ما تتعبيش + +61 +00:05:48,830 --> 00:05:54,710 +و برضه تحصلي على ان شاء الله الدرجة كاملة و + +62 +00:05:54,710 --> 00:05:59,050 +يعطيكوا العافيةالسلام عليكم ورحمة الله وبركاته + diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/b74Uqmmp7mw_raw.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/b74Uqmmp7mw_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..0a87b6a6c61b946a9a677a04eba24da2fdedf6c4 --- /dev/null +++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/b74Uqmmp7mw_raw.srt @@ -0,0 +1,600 @@ +1 +00:00:07,640 --> 00:00:10,360 +أعوذ بالله من الشيطان الرجيم بسم الله الرحمن + +2 +00:00:10,360 --> 00:00:15,460 +الرحيم إن الحمد لله نحمده ونستعينه ونستغفره ونعوذ + +3 +00:00:15,460 --> 00:00:19,560 +بالله من شرور أنفسنا وسيئات أعمالنا ما يهدي الله + +4 +00:00:19,560 --> 00:00:23,160 +فلا مضل له وما يضلل فلا هادي له وأشهد أن لا إله + +5 +00:00:23,160 --> 00:00:28,310 +إلا الله وأشهد أن محمدا عبده ورسوله وبعدبداية + +6 +00:00:28,310 --> 00:00:32,410 +أخوات الحبيبات وبنات الطيبات أحييكم بتحية طيبة + +7 +00:00:32,410 --> 00:00:36,730 +مباركة من عند الله السلام عليكم ورحمة الله وبركاته + +8 +00:00:36,730 --> 00:00:39,810 +اللهم أعلمنا ما ينفعنا وانفعنا بما علمتنا وازدنا + +9 +00:00:39,810 --> 00:00:45,570 +علما اللهم آمين كنا قد تحدثنا في المحاضرة السابقة + +10 +00:00:45,570 --> 00:00:50,450 +عن أهلية الوجوب وأحوال أهلية الوجوب وأقسام أهلية + +11 +00:00:50,450 --> 00:00:56,160 +الوجوب وعرفنا أن أساس أهلية الوجوب هي الحياةاليوم + +12 +00:00:56,160 --> 00:01:01,280 +ان شاء الله هنتكلم عن أهلية الأداء بالنسبالي أهلية + +13 +00:01:01,280 --> 00:01:06,860 +الأداء تنقسم إلى ثلاثة أقسام عندي أهلية أداء + +14 +00:01:06,860 --> 00:01:14,000 +منعدمة أهلية أداء ناقصة أهلية أداء كاملة بالنسبالي + +15 +00:01:14,000 --> 00:01:18,980 +حقيقة أهلية الأداء يعني تعريف أهلية الأداء أو معنى + +16 +00:01:18,980 --> 00:01:24,050 +أهلية الأداء أهلية الأداء صلاحية الإنسانلصدور + +17 +00:01:24,050 --> 00:01:30,330 +الأفعال والأقوال منه على وجه يعتد به شرعا ويترتب + +18 +00:01:30,330 --> 00:01:36,510 +عليه آثاره الشرعية وعلى هذا فإن أهلية الأداء كما + +19 +00:01:36,510 --> 00:01:42,030 +يقول العلماء هي أهلية المعاملة يعني أهلية الأداء + +20 +00:01:42,030 --> 00:01:47,470 +كمان مرة هي صلاحية الإنسان لصدور الأفعال والأقوال + +21 +00:01:47,470 --> 00:01:54,050 +منهعلى وجه يعتد به شرعا خد بالك من العبارة هذه على + +22 +00:01:54,050 --> 00:02:00,030 +وجه يعتد به شرعا ويترتب عليه آثاره الشرعية عشان + +23 +00:02:00,030 --> 00:02:04,470 +هيك قالوا أن أهلية الأداء هذه هي أهلية المعاملة + +24 +00:02:04,470 --> 00:02:08,590 +حيث يكون الإنسان صالحا لاكتساب حقوق فيما يقوم به + +25 +00:02:08,590 --> 00:02:14,370 +من تصرفات وصالحا لأن ينشئ حقوق لغيره بتلك التصرفات + +26 +00:02:14,370 --> 00:02:20,200 +وهذه اللي بنسميها الواجباتمن خلال التعريفات + +27 +00:02:20,200 --> 00:02:25,020 +السابقة نجد أن أهلية الأداء تعوم المعاملة + +28 +00:02:25,020 --> 00:02:30,240 +والمعاملات والعبادات تعوم المعاملات والعبادات يعني + +29 +00:02:30,240 --> 00:02:37,760 +الإنسان لابد أن يكون ليه يصلي أهلاً لصدور فعل + +30 +00:02:37,760 --> 00:02:44,270 +الصلاة منه حتى يعتدواهذا التصرف منه ويترتب عليه + +31 +00:02:44,270 --> 00:02:50,650 +آثاره وهو براء الذمة من الصلاة وكذلك في المعاملات + +32 +00:02:50,650 --> 00:02:56,730 +إذا أنشأ عقدا لابد أن يكون أهلا أو صالحا لصدور هذا + +33 +00:02:56,730 --> 00:03:02,130 +العقد منه على وجه يعترف به المشرع وتترتب عليه + +34 +00:03:02,130 --> 00:03:08,110 +آثاره من تسليم المبيع واستلام الثمن + +35 +00:03:09,740 --> 00:03:15,800 +نجي لأساس أهلية الأداء، قلنا أساس أهلية الوجوبية + +36 +00:03:15,800 --> 00:03:20,700 +الحياة، متفق العلماء على أن أساس أهلية الأداء هو + +37 +00:03:20,700 --> 00:03:26,380 +التمييز والعقل، إذا لو كتبنا أهلية الوجوب تساوي أو + +38 +00:03:26,380 --> 00:03:31,350 +أساسها تساوي الحياةأهلية الأداء أساسها التمييز + +39 +00:03:31,350 --> 00:03:36,510 +والعقل فأهلية الأداء لا تثبت إلا بالعقل وقدرة + +40 +00:03:36,510 --> 00:03:42,090 +العمل به وبدون العقل لا توجد أهلية أداء نيجي الآن + +41 +00:03:42,090 --> 00:03:46,110 +لأحوال أهلية الأداء طبعا الكلام السابق اللي حكيناه + +42 +00:03:46,110 --> 00:03:51,890 +كلام مهم جدا جدا جدا جدا الآن الأحوال عندي تلت + +43 +00:03:51,890 --> 00:03:58,730 +حالات حالة عدم أهلية الأداء مطلقاحالة وجود أهلية + +44 +00:03:58,730 --> 00:04:03,970 +أداء لكن هذه الأهلية ناقصة حالة وجود أهلية أداء + +45 +00:04:03,970 --> 00:04:09,270 +لكن هذه الأهلية تعتبر كاملة خلينا نشوف الحالة + +46 +00:04:09,270 --> 00:04:15,490 +الأولى وهي حالة انعدام أهلية الأداء مطلقاو ذلك + +47 +00:04:15,490 --> 00:04:21,590 +متحقق في الطفل اللذي لا يميز و أيضا في المجنون إذا + +48 +00:04:21,590 --> 00:04:26,990 +ما استمر جنونه إذا كلاهما عديم العقل إذا إحنا + +49 +00:04:26,990 --> 00:04:31,730 +حكينا أهلية الأداء أساسها إيش وجود العقل و التمييز + +50 +00:04:31,730 --> 00:04:35,910 +في حالة أهلية الأداء المنعدمة إذا لا في عقل ولا في + +51 +00:04:35,910 --> 00:04:39,230 +تميز و هذا الكلام متحقق في الطفل الصغير اللذي لا + +52 +00:04:39,230 --> 00:04:46,130 +يميز و في المجنونفكلهما عديم العقل، ومن لا عقل له، + +53 +00:04:46,130 --> 00:04:50,730 +لا أهلية له المجنون + +54 +00:04:50,730 --> 00:04:55,730 +والصخير غير المميز، لو أجرى عقدا من العقود، إجراء + +55 +00:04:55,730 --> 00:05:00,130 +هذا تبعه باطل، وعقد هذا تبعه باطل، وتصرفاته كلها + +56 +00:05:00,130 --> 00:05:05,270 +الشرعية باطلة، ولا يترتب على قوله أو فعله أي أثر + +57 +00:05:05,270 --> 00:05:10,390 +شرعيفجنايتهما اللي هم المجنون والطفل وهذا كله + +58 +00:05:10,390 --> 00:05:14,790 +هنفصل فيه لما نتكلم عن عوارض الأهلية جنايتهما على + +59 +00:05:14,790 --> 00:05:19,030 +النفس أو المال تترتب عليها المؤاخذة المالية وحدها + +60 +00:05:19,030 --> 00:05:23,890 +زي ما حكينا أن الطفل الصغير يرعاه الولي والمجنون + +61 +00:05:23,890 --> 00:05:28,760 +يرعاه الوليوقتل الطفل أو المجنون أو إتلافه حق + +62 +00:05:28,760 --> 00:05:32,820 +الغير يترتب عليه ضمان الديا في القتل وضمان التلف + +63 +00:05:32,820 --> 00:05:37,560 +فيما أتلفه ولا ينبني علافي عليهم قصاص لأنه مترتب + +64 +00:05:37,560 --> 00:05:42,440 +على العمد ووجود القصد وكلهما قد انعدب من عدم + +65 +00:05:42,440 --> 00:05:49,480 +القدرتين والإنسان في أحواله عديم القدرتين العقل + +66 +00:05:49,480 --> 00:05:55,340 +والبدن لكن فيه استعداد وصلاحية لأنتوجد فيه كل + +67 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أداء ناقصة لأن هذا النقصان + +79 +00:07:05,910 --> 00:07:10,650 +منبني على القدرة القاصرة والناقصة من العقلالقاصر + +80 +00:07:10,650 --> 00:07:16,570 +والبدن القاصر فالطفل الصبي صح هو مميز لكن لسه لم + +81 +00:07:16,570 --> 00:07:22,670 +يكتمل نموه ليكتمل تمييزه ويكتمل عقله أيضا المعتوه + +82 +00:07:22,670 --> 00:07:28,150 +عنده خلل وضعف في الإدراك يعني موجود في عقل لكن هذا + +83 +00:07:28,150 --> 00:07:34,390 +العقل ناقص وضعيف فهنا تثبت الأهلية لهما لثبوت أصل + +84 +00:07:34,390 --> 00:07:38,390 +الأهلية بالتمييز لكنها تثبت ناقصها عشان هيك تصح + +85 +00:07:38,390 --> 00:07:45,710 +منهمابعض التصرفات وتكون موقوفة على إذن الولي وطبطل + +86 +00:07:45,710 --> 00:07:51,210 +بعض التصرفات حتى وإن أجازها الولي عشان هيك لو جينا + +87 +00:07:51,210 --> 00:07:57,110 +نعرف أهلية الأداء الناقصة هي صلاحية الشخص لصدور + +88 +00:07:57,110 --> 00:08:02,750 +بعض التصرفات منهم دون البعض الآخرأو لصدور أفعال و + +89 +00:08:02,750 --> 00:08:07,550 +تصرفات يتوقف نفاذها على رأي غيره زي ما حكينا قبل + +90 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00:00:31,820 +بداية أخوات الحبيبات وبنات الطيبات أحييكم بتحية + +7 +00:00:31,820 --> 00:00:36,420 +طيبة مباركة من عند الله السلام عليكم ورحمة الله + +8 +00:00:36,420 --> 00:00:42,980 +وبركاته سنتحدث اليوم في هذه المحاضرة عن المحكوم + +9 +00:00:42,980 --> 00:00:51,660 +فيه أولا المحكوم فيه وهو فعل المكلف طبعا حقيقة + +10 +00:00:51,660 --> 00:00:58,520 +المحكوم فيه هو الفعل الذي تعلقبه خطاب الشارع زي + +11 +00:00:58,520 --> 00:01:05,840 +الإجاب متعلق الواجب الندب متعلق المندوب التحريم + +12 +00:01:05,840 --> 00:01:12,480 +متعلق الحرام أو المحرم الكراهة ومتعلقها المكروه + +13 +00:01:12,480 --> 00:01:18,920 +الإباحة ومتعلقها المباح وسبق أن تحدثنا عن الإجاب + +14 +00:01:18,920 --> 00:01:24,600 +وتعريفه والواجب وتعريفه واستعرضنا الأمثلة التي + +15 +00:01:25,120 --> 00:01:30,960 +تتعلق بحقيقة المحكوم فيه أو حقيقة الفعل الذي تعلق + +16 +00:01:30,960 --> 00:01:36,220 +به خطاب الشارع وهذا الكلام كمان اللي بدي أحكيه + +17 +00:01:36,220 --> 00:01:42,080 +الآن مش جديد لكن خلّينا احنا نستعرضه المحكوم فيه + +18 +00:01:42,080 --> 00:01:46,720 +إذا كان خطاب الشارع من قبيل الحكم التكليفي فإن + +19 +00:01:46,720 --> 00:01:51,440 +المحكوم فيه لا يكون إلا فعلا المكلف زي الصلاة + +20 +00:01:51,440 --> 00:01:58,450 +الصومكتابة الدين القتل العمد العدوان طبعا هذا + +21 +00:01:58,450 --> 00:02:03,810 +الكلام له أدلة كثيرة كقول الله تعالى وأقيموا + +22 +00:02:03,810 --> 00:02:09,090 +الصلاة واتوا الذكاء فيه طبعا إيجاب تعلق بفعل وهو + +23 +00:02:09,090 --> 00:02:14,270 +إقامة الصلاة فجعله واجبا وهي كمان مرة بنقول أن + +24 +00:02:14,270 --> 00:02:20,550 +الكلام هذا مش جديد هذا كلام يعني سبق أن تحدثنا به + +25 +00:02:21,310 --> 00:02:24,710 +وقال الله تعالى يا أيها الذين آمنوا إذا تدايمتم + +26 +00:02:24,710 --> 00:02:30,850 +بدين إلى أجل المسمى، مسما فاكتبوه طبعا فيه ندب + +27 +00:02:30,850 --> 00:02:35,770 +تعلق بفعل وهو كتابة الدين فجعله مندوبا فعل مين + +28 +00:02:35,770 --> 00:02:42,110 +طبعا فعل المكلف وقوله أيضا وقول الله تعالى ولا + +29 +00:02:42,110 --> 00:02:47,870 +تقتلوا نفس التحريم المستفاد منه تعلق بفعل هو قتل + +30 +00:02:47,870 --> 00:02:52,990 +النفس فجعله محرماوقوله عز وجل فإذا قضيت الصلاة + +31 +00:02:52,990 --> 00:02:56,830 +فانتشره في الأرض فيه إباحة وهو الانتشار في الأرض + +32 +00:02:56,830 --> 00:03:04,030 +وذلك متعلق بفعل المكلف فجعله مباحا أما إذا كان من + +33 +00:03:04,030 --> 00:03:08,390 +قبيل الحكم الوضعي فقد يكون المحكم فيه فعل المكلف + +34 +00:03:08,390 --> 00:03:13,830 +وقد لا يكون فعل المكلف ولكن له ارتباط بفعله إذا + +35 +00:03:13,830 --> 00:03:17,350 +كان المحكم فيه فعل المكلف زي الطهارة التي جعلها + +36 +00:03:17,350 --> 00:03:22,930 +المشرع شرطا للصلاةوهي فعل للمكلف مين اللي بده يعمل + +37 +00:03:22,930 --> 00:03:25,550 +التهارة هذه؟ مين اللي بده يتوضى؟ اللي هو مين؟ + +38 +00:03:25,550 --> 00:03:32,530 +المكلف والزنا الذي جعله الشارع سببا للوجوب طبعا + +39 +00:03:32,530 --> 00:03:38,950 +الحد وهو فعل للمكلف قتل الوارث مورسه الذي جعله + +40 +00:03:38,950 --> 00:03:43,050 +الشارع مانع من الميراث وهو فعل للمكلف أيضا البيع + +41 +00:03:43,050 --> 00:03:49,950 +جعله الشارع سببا للملكية وهو فعل للمكلفوقد لا يكون + +42 +00:03:49,950 --> 00:03:57,730 +فعلًا للمكلف ولكن له ارتباط بفعله كدلوق الشمس فإنه + +43 +00:03:57,730 --> 00:04:01,350 +ليس فعل من أفعال المكلف و لا نستطيع نطلع الشمس و + +44 +00:04:01,350 --> 00:04:05,930 +نرجع الشمس نخليها تشرق و نخليها تغيب، لأ طبعًا هذه + +45 +00:04:05,930 --> 00:04:11,090 +ليست من أفعال المكلفين لكن مرتبطة بفعل المكلف من + +46 +00:04:11,090 --> 00:04:15,550 +ناحية أن الدلوق سبب لوجوب الصلاة التي هي من أفعال + +47 +00:04:15,550 --> 00:04:21,730 +المكلفأيضًا بلوغ الحلم الذي جعله الشارع شرطان + +48 +00:04:21,730 --> 00:04:26,330 +للتكليف ليس فعلا من أفعال المكلف ولكنه مرتبط بفعل + +49 +00:04:26,330 --> 00:04:32,930 +المكلف من ناحية أن بلوغ الحلم سببًا لأهلية التكليف + +50 +00:04:32,930 --> 00:04:38,270 +وكذلك الحيد والنفاس اللذان جعلهم الشارع مانعين من + +51 +00:04:38,270 --> 00:04:43,070 +صحة العبادة من هنا نفهم أنه لا تكليف إلا بفعل كما + +52 +00:04:43,070 --> 00:04:49,330 +يقول علماء الأصولفإذا كان حكم الشارع إيجابا أو + +53 +00:04:49,330 --> 00:04:53,730 +ندبا فمتعلق الإيجاب هو فعل الواجب على سبيل الحتم + +54 +00:04:53,730 --> 00:04:58,850 +ومتعلق الندب هو فعل المندوب لعل سبيل الحتم ففي + +55 +00:04:58,850 --> 00:05:03,950 +الحالتين يكون تكليفا بفعل وكذلك التحريم والكرهة + +56 +00:05:03,950 --> 00:05:09,430 +فالمكلف بيه فيهما فعل لأنه عبارة عن كف النفس عن + +57 +00:05:09,430 --> 00:05:17,580 +الفعل المحرم أو ال macroوضع العلماء شروط للمحكوم + +58 +00:05:17,580 --> 00:05:22,960 +فيه خلّينا نستعرض هذه الشروط عندنا اشترطوا لصحة + +59 +00:05:22,960 --> 00:05:27,620 +التكليف بالفعل تلت شروط وهناك شرط رابع مختلف فيه + +60 +00:05:27,620 --> 00:05:33,120 +مش هنتكلم عنه أما الشرط الأول يشترط أن يكون الفعل + +61 +00:05:33,120 --> 00:05:39,800 +معلوما للمبلغ علما داما حتى يتصور قصده إليه ويؤديه + +62 +00:05:39,800 --> 00:05:45,160 +على الوجه المطلوب منهفلا يكلف الشخص بالصلاة حتى + +63 +00:05:45,160 --> 00:05:49,820 +يعرف أركان الصلاة وشروط الصلاة وكيفيت أدا الصلاة + +64 +00:05:49,820 --> 00:05:54,580 +حيث أن الأمر + +65 +00:05:54,580 --> 00:06:00,460 +بالصلاة في القرآن جاء مجملا قال تعالى وأقيموا + +66 +00:06:00,460 --> 00:06:04,300 +الصلاة ومن الذي بين هذا المجمل؟ النبي صلى الله + +67 +00:06:04,300 --> 00:06:10,820 +عليه وسلم بينه بفعله في الصلاة كما قال للصحابة رضي + +68 +00:06:10,820 --> 00:06:15,830 +الله عنهمصلوا كما رأيتموني و صليت و هيك علمهم و + +69 +00:06:15,830 --> 00:06:21,390 +صارت الصلاة معلومة لديهم من حيث أركانها و شروطها و + +70 +00:06:21,390 --> 00:06:28,210 +كيفيتها و كذلك الذكاء و الصوم و الحج فكل هذه أمور + +71 +00:06:28,210 --> 00:06:32,990 +جاءت مجملة و النبي صلى الله عليه و سلم بين حقيقتها + +72 +00:06:32,990 --> 00:06:38,190 +و أركانها و شروطها و مقدارها و وقتها حتى أصبحت + +73 +00:06:38,190 --> 00:06:44,310 +معلومة للمكلف علما تمامإذا شروط المحكم فيها أن + +74 +00:06:44,310 --> 00:06:50,850 +يكون الفعل معلوما للمكلف علما تاما حتى يتصور قصده + +75 +00:06:50,850 --> 00:06:55,550 +إليه ويؤديه على الوجه المطلوب مش معقول واحد حاجة + +76 +00:06:55,550 --> 00:07:00,590 +ما بعرفاش ييجي تقوله اعملها ويقدر ينفذها لازم يكون + +77 +00:07:00,590 --> 00:07:06,830 +يتوضح له هذا الأمروزي ما النبي صلى الله عليه وسلم + +78 +00:07:06,830 --> 00:07:12,250 +فعل مع الصحابة وضح لهم كيفية الصلاة وأركان الصلاة + +79 +00:07:12,250 --> 00:07:19,050 +وشروطها وكيفية أداءه وبعد ذلك طالبهم بالصلاة + +80 +00:07:19,050 --> 00:07:26,210 +السؤال الآن ما المراد بعلم المكلف بما كلف به؟ طبعا + +81 +00:07:26,210 --> 00:07:32,630 +هنا المراد هو يمكن علمه به وليس المراد علمه به + +82 +00:07:32,630 --> 00:07:39,320 +فعلاإيش يعني؟ يعني الإنسان الذي يصل إلى سن البلوغ + +83 +00:07:39,320 --> 00:07:45,320 +هو عاقل قادر على معرفة الأحكام الشرعية بنفسه وإذا + +84 +00:07:45,320 --> 00:07:50,580 +مش قادر هو بنفسه يعرف الأحكام هذه، متوجه للعلماء + +85 +00:07:50,580 --> 00:07:56,900 +بالسؤال هنا يعتبر عالمًا بما كلف به وتنفذ عليه + +86 +00:07:56,900 --> 00:08:02,780 +الأحكام ويلزم بآثارها ولا يقبل منه الاعتذار بالجهل + +87 +00:08:03,200 --> 00:08:07,760 +يعني مينفعش واحد نقوله صلي يقول بعرفش أصلي لأ بدك + +88 +00:08:07,760 --> 00:08:13,580 +تروح تقرا عن الصلاة كيف أحكامها و تتعلمها بنفسك و + +89 +00:08:13,580 --> 00:08:18,160 +مش تعرفش من حالك بتروح إيش بتتوجه للعلماء و + +90 +00:08:18,160 --> 00:08:21,900 +بتسألهم عشان هيك قال الفقهاء لا يقبل في دار + +91 +00:08:21,900 --> 00:08:26,780 +الإسلام عذر بالجهل للحكم الشرعي وبهذا الإمكان + +92 +00:08:26,780 --> 00:08:32,340 +يستطيع المكلف القيام بما كلف بهوالسبب في الاكتفاء + +93 +00:08:32,340 --> 00:08:37,520 +بإمكان العلم بالحكم هو أنه لو شرطه لصحة التكليف + +94 +00:08:37,520 --> 00:08:42,180 +علم المكلف بالفعل لا إمكانه لتسعى طبعا مجال + +95 +00:08:42,180 --> 00:08:47,220 +الاعتذار بجهل الأحكام ولا مستقامة التكليف وفي هذا + +96 +00:08:47,220 --> 00:08:54,940 +تعطيل للأحكم أما بالنسبة للشرط التاني طبعا احنا + +97 +00:08:54,940 --> 00:08:59,570 +عشان نذكركم احنا بنتكلم في شروط المحكم فيهخلصنا + +98 +00:08:59,570 --> 00:09:04,770 +الشرط الأول، ننتقل الآن إلى الشرط الثاني أن يعلم + +99 +00:09:04,770 --> 00:09:10,810 +المكلف طلب الله تعالى للفعل حتى يعتبر طاعة وامتثال + +100 +00:09:10,810 --> 00:09:15,430 +لأوامر الله تعالى علشان هيك المسلم لا يقبل حكما + +101 +00:09:15,430 --> 00:09:19,950 +إلا إذا علم أن الله سبحانه وتعالى قد طلبه ليتصور + +102 +00:09:19,950 --> 00:09:25,430 +قصد الطاعة وامتثال لله تعالى فيه أما هذا طبعا + +103 +00:09:25,430 --> 00:09:30,970 +الشرط واضح، مافيدهشيعني شرح كتير أما الشرط التالث + +104 +00:09:30,970 --> 00:09:36,670 +أن يكون الفعل المكلف به ممكن بحيث يستطيع المكلف أن + +105 +00:09:36,670 --> 00:09:41,970 +يفعله أو يتركه إيش يعني أن يكون الفعل المكلف به + +106 +00:09:41,970 --> 00:09:48,610 +ممكن يعني يستطيع المكلف فعله بدون مشقة يعني لو + +107 +00:09:48,610 --> 00:09:56,050 +قلنا إن صلاة الفجر لازم نصليها على صور الحيط تمام؟ + +108 +00:09:56,380 --> 00:10:00,760 +أكم واحد حيصلّي على سور الحيط صلاة الفجر؟ قليل، + +109 +00:10:00,760 --> 00:10:04,940 +واللي حيصلّي يوم عن يوم هيتعب، هيبطل يصلّي، إذا + +110 +00:10:04,940 --> 00:10:08,940 +عشان هيك، بدو يكون الفعل إيه؟ بدو يكون ممكن، يعني + +111 +00:10:08,940 --> 00:10:13,860 +يستطيع المكلف أن ينفذه بدون كلفة، بدون مشقة وبدون + +112 +00:10:13,860 --> 00:10:20,460 +تعب طبعا، مجال التكليف الشرعي للأفعال التي لتخرج + +113 +00:10:20,460 --> 00:10:27,290 +عن طاعة المكلفين، طاقة المكلفين ووصهموما ليس فيه + +114 +00:10:27,290 --> 00:10:31,450 +من المشقة والعناء ما يخرج عن المعهود في الأعمال + +115 +00:10:31,450 --> 00:10:35,790 +العادية طبعا لما ربنا سبحانه وتعالى يطلب مننا معنى + +116 +00:10:35,790 --> 00:10:40,890 +الكلام هذا يطلب مننا الصلاة صلاة سهلة أني أنا كل + +117 +00:10:40,890 --> 00:10:46,270 +ما علي أني أطهر للصلاة هذه و أبدأ في فعل الصلاة + +118 +00:10:46,270 --> 00:10:52,950 +مرات بتقول يمكن هذا الفعل تقيل وفي مشقة اه المشقة + +119 +00:10:52,950 --> 00:10:59,130 +نوعين مشقةممكنة ومشقة غير ممكنة مشقة ممكنة يعني + +120 +00:10:59,130 --> 00:11:05,110 +يستطيع المكلف أن يفعلها لكن مشقة غير ممكنة هذه لا + +121 +00:11:05,110 --> 00:11:10,830 +يستطيع المكلف أن يفعلها وبالتالي سيترك هذه العبادة + +122 +00:11:10,830 --> 00:11:17,410 +لكن المشرع يعني أن المكلف يقوم بالعبادة وينفذ + +123 +00:11:17,410 --> 00:11:22,890 +العبادة حتى يقوم بإعمار الأرض طبعا يمكن الفعل يكون + +124 +00:11:22,890 --> 00:11:28,680 +ثقيل على النفسإن بدي أصحى من النوم و أتودى و أصلي + +125 +00:11:28,680 --> 00:11:33,640 +هذا فعل يعني ممكن يكون أي فعل يصير ممكن يكون ثقيل + +126 +00:11:33,640 --> 00:11:41,280 +على النفس لكن هنا وإن أحس المكلف بعبق لم يكن يحس + +127 +00:11:41,280 --> 00:11:46,040 +به قبل التكليف هذا الشعور بنسميه شعور فطري لا يمكن + +128 +00:11:46,040 --> 00:11:52,480 +رفعه شعور فطري يعني كل واحد ممكن يتعبمن أنه أو يجد + +129 +00:11:52,480 --> 00:11:58,980 +مشقة من أنه يقوم للصلاة ويتوضى ويصلي لكن هذا + +130 +00:11:58,980 --> 00:12:04,480 +الشعور المشقة بسيط ويمكن يتغلب عليه الإنسان وهو + +131 +00:12:04,480 --> 00:12:10,990 +شعور فطري لا يمكن يعني إزالته من نفس الإنسانإن نفس + +132 +00:12:10,990 --> 00:12:16,990 +الإنسان حب الإنسان الراحة والخلود إلى يعني العدم + +133 +00:12:16,990 --> 00:12:24,290 +القيم من النوم وعدم فعل أي أمر يسبب له تعب هذا + +134 +00:12:24,290 --> 00:12:29,450 +التعب وإن كان بسيط لكن المشارع يعلم أن المكلف ممكن + +135 +00:12:29,450 --> 00:12:33,290 +أن يتغلب على هذا التعب وعلى هذا الشعور الفطري + +136 +00:12:33,290 --> 00:12:39,750 +ويحقق العبادةهنا عندنا بالنسبة لأقسام المحكم فيه + +137 +00:12:39,750 --> 00:12:44,290 +مش هناخده بالتفصيل لكن هنستعرضه استعراض الحنفية + +138 +00:12:44,290 --> 00:12:49,090 +قسم وفعل المكلف من حيث صلته بأحكام الله تعالى + +139 +00:12:49,090 --> 00:12:53,990 +وحقوق العباد إلى أربع أقسام حق خالص لله تعالى، حق + +140 +00:12:53,990 --> 00:12:58,830 +خالص للعبد، مجتمع فيه الحقان وحق الله غالب، مجتمع + +141 +00:12:58,830 --> 00:13:03,800 +فيه الحقان وحق العبد غالبهذه تقسيم الحقوق أو تقسيم + +142 +00:13:03,800 --> 00:13:09,460 +الأقصام المحكوم فيها عند الحنافية بس يعني إعرفوهم + +143 +00:13:09,460 --> 00:13:14,520 +معرفة بدون تفصيل بدون تعريف لكل حق من الحقوق هذه + +144 +00:13:14,520 --> 00:13:20,600 +بدون أن نستعرض أمثلة على هذه الحقوق هذا بس للإعلان + +145 +00:13:20,600 --> 00:13:25,000 +فقط الآن + +146 +00:13:25,000 --> 00:13:31,560 +سنتحدث عن المحكوم عليه المحكوم عليه الآن هو المكلف + +147 +00:13:32,040 --> 00:13:38,220 +ومن هنا بدنا نبدأ طبعا الكلام المهم الذي يتعلق + +148 +00:13:38,220 --> 00:13:44,640 +بالمكلف المكلف هو الشخص البلغ العقل الذي تعلق خطاب + +149 +00:13:44,640 --> 00:13:51,060 +الشارع بفعله ووجه إليه الأحكام وطالبه بتنفيذها شرط + +150 +00:13:51,060 --> 00:13:56,120 +صحة التكليف أو شرط صحة التكليف المكلف طبعا عندي + +151 +00:13:56,120 --> 00:14:00,400 +شرطين ومهمين هذول الشرطين الشرط الأول اللي هو + +152 +00:14:00,400 --> 00:14:04,320 +العقل والفهموالشرط الثاني اللي هو الأهلية اللي + +153 +00:14:04,320 --> 00:14:09,820 +هنتكلم عنها ان شاء الله بتفصيل كبير في الفصل + +154 +00:14:09,820 --> 00:14:15,100 +الأخير اللي هو الفصل التالت ان شاء الله تعالى أما + +155 +00:14:15,100 --> 00:14:19,040 +بالنسبة ليه الشرط الأول العقل والفهم؟ يعني أن يكون + +156 +00:14:19,040 --> 00:14:23,360 +المكلف قادرا على فهم دليل التكليف بأن يفهم الكلام + +157 +00:14:23,360 --> 00:14:27,140 +الذي وجهه إليه والمراد بإن الفهم استطاعت فهم + +158 +00:14:27,140 --> 00:14:32,600 +المكلف للخطاب وتصوره وليس تصديق بهعشان هيك بنقول + +159 +00:14:32,600 --> 00:14:37,080 +الكفار مخاطبون بالإيمان وإن لم يقع منهم تصديق + +160 +00:14:37,080 --> 00:14:40,580 +باتفاق العلماء لأن الرسول صلى الله عليه وسلم مبعوث + +161 +00:14:40,580 --> 00:14:44,960 +إلى جميع الخلق عشان هيك يقول أحد علماء الأصول وهو + +162 +00:14:44,960 --> 00:14:49,300 +الشوكاني لا خلافة في أنهم مخاطبون يعني الكفار بأمر + +163 +00:14:49,300 --> 00:14:53,080 +الإيمان لأنه النبي صلى الله عليه وسلم مبعوث إلى + +164 +00:14:53,080 --> 00:14:59,720 +الكافة و بالمعاملات أيضا دليل هذا الشرطلزوم المحال + +165 +00:14:59,720 --> 00:15:05,480 +عند عدم اعتباره في التكليف كيف؟ لأن طلب حصول الفعل + +166 +00:15:05,480 --> 00:15:09,960 +من المكلف يقصد به الامتثال والابتلاء وذلك مستحيل + +167 +00:15:09,960 --> 00:15:15,640 +عادة وشرعا ممن لا شعور له ولأنه لو صح ذلك التكليف + +168 +00:15:15,640 --> 00:15:20,520 +لكل فترة البهائم التي حالة عدم الفهم بينها وبين + +169 +00:15:20,520 --> 00:15:26,630 +الخطاب يعني لابد أن يوجد العقل والفهم حتىيتم تكليف + +170 +00:15:26,630 --> 00:15:29,970 +المكلف بترتب على هذا الشرط عدة أمور هذه الأمور + +171 +00:15:29,970 --> 00:15:33,650 +اللي هنستعرضها سريعا لها تفصيل في الحديث عن + +172 +00:15:33,650 --> 00:15:39,650 +الأهلية عدم تكليف طبعا المجنون والصبي هذا إشي يعني + +173 +00:15:39,650 --> 00:15:46,550 +بديهي كيف نكلف من لا عقل له ومن لا إدراك له وهذا + +174 +00:15:46,550 --> 00:15:50,890 +هنتكلم ان شاء الله بالتفصيل عنه عدم تكليف النائم + +175 +00:15:50,890 --> 00:15:56,300 +والغافل والسكرانبرضه لنا حديث طويل عنهم طبعا عدم + +176 +00:15:56,300 --> 00:16:02,480 +تكليفهم بالعبادة لكن النفقة للأقارب والزكاة وجوب + +177 +00:16:02,480 --> 00:16:05,940 +الضمان فيما يتلفه الصبي والمجنون ليس من خطاب + +178 +00:16:05,940 --> 00:16:09,900 +التكليف إنما من خطاب الوضع اللي هو ربط الأسباب + +179 +00:16:09,900 --> 00:16:14,140 +بالمسببات لأن هذه الواجبات لم تتعلق بفعل الصبي + +180 +00:16:14,140 --> 00:16:19,000 +والمجنون إنما تعلقت بالمال أو الذمة وهذا برضه + +181 +00:16:19,000 --> 00:16:23,670 +هنوضحه أكتر لما نتكلم عنأهلية الصبي وأهلية المجنون + +182 +00:16:23,670 --> 00:16:30,810 +ليش؟ لأنهم صالحان بما ثبت لهم من إنسانية تهيئتهم + +183 +00:16:30,810 --> 00:16:36,310 +لفهم الخطاب عند حدوث البلوغ والإفاقة ومتول الأداء + +184 +00:16:36,310 --> 00:16:42,550 +هو الولي بعد بلوغ الصبي وإفاقة المجنون كمان مرة + +185 +00:16:42,550 --> 00:16:48,690 +بقول إن هذا الكلام هنقوم بتفصيلهإن شاء الله عندها + +186 +00:16:48,690 --> 00:16:52,730 +في الفصل الثالث وهو الأخير معناه بيكون إن شاء الله + +187 +00:16:52,730 --> 00:16:57,170 +الشرط التاني الأهلية طبعاً أن يكون المكلف أهلا + +188 +00:16:57,170 --> 00:17:01,330 +للتكليف وأهلية المكلف لا تتحقق إلا بالعقل والفهم + +189 +00:17:01,330 --> 00:17:07,550 +لأن العقل شرط للتكليف برضه موضوع الأهلية إن شاء + +190 +00:17:07,550 --> 00:17:13,740 +الله نفصل الحديث فيه بتعريف الأهليةوسنتكلم عن + +191 +00:17:13,740 --> 00:17:19,060 +عوارض الأهلية السماوية والمكتسبة بإذن الله تعالى + +192 +00:17:19,060 --> 00:17:25,140 +كما قلنا في الفصل الثالث بإذن الله تعالى نكتفي + +193 +00:17:25,140 --> 00:17:29,740 +بهذا القدر في هذه المحاضرة على أن نلقاكم في + +194 +00:17:29,740 --> 00:17:34,180 +المحاضرة القادمة السلام عليكم ورحمة الله وبركاته + diff --git a/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zJ_ZuDD_IQs_postprocess.srt b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zJ_ZuDD_IQs_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..fc6cafd3897da70cac66509b8df0e921653ebde2 --- /dev/null +++ b/PL9fwy3NUQKwa696OVQhdP6KIv_ixbCaej/zJ_ZuDD_IQs_postprocess.srt @@ -0,0 +1,596 @@ +1 +00:00:11,240 --> 00:00:13,920 +أعوذ بالله من الشيطان الرجيم بسم الله الرحمن + +2 +00:00:13,920 --> 00:00:17,560 +الرحيم الحمد لله الواحد الأحد الفرد الصمد الذي لم + +3 +00:00:17,560 --> 00:00:21,480 +يلد ولم يولد ولم يكن له كفوا أحد وصلاة والسلام على + +4 +00:00:21,480 --> 00:00:27,160 +خير الأنام محمد وعلى آله وصحبه الكرام وبعد بداية + +5 +00:00:27,160 --> 00:00:31,040 +أخوات الحبيبات وبنات الطيبات أحييكم بتحية الإسلام + +6 +00:00:31,040 --> 00:00:35,150 +العظيم السلام عليكم ورحمة الله وبركاتهكنا قد + +7 +00:00:35,150 --> 00:00:40,330 +انتهينا في المحاضرة السابقة من الحديث عن أقسام + +8 +00:00:40,330 --> 00:00:46,770 +الأهلية وقولنا أن أقسام الأهلية قسمين الأول أهلية + +9 +00:00:46,770 --> 00:00:50,990 +الوجوب والثاني أهلية الأداء طبعا أهلية الوجوب + +10 +00:00:50,990 --> 00:00:56,590 +أهلية ناقصة وكاملة وأهلية الأداء منعدمة وناقصة + +11 +00:00:56,590 --> 00:01:01,540 +وكاملة وفصلنا الحديث عن ذلكاليوم ان شاء الله + +12 +00:01:01,540 --> 00:01:08,880 +هنتكلم عن عوارض الأهلية بالنسبة لمعنى العوارض في + +13 +00:01:08,880 --> 00:01:14,940 +اللغة العارض هو المانع أو الحائل ومعنى اعترض الشيء + +14 +00:01:14,940 --> 00:01:20,760 +صار عارضا واعترض دونه حالة واعترض له أي منعه + +15 +00:01:20,760 --> 00:01:26,300 +والعارض أيضا ما اعترض في الأفق فسده من سحاب او نحل + +16 +00:01:26,300 --> 00:01:31,760 +او جراد او نحو ذلك ومنه قول اللهتعالى عن قوم عاد + +17 +00:01:31,760 --> 00:01:37,620 +هذا عارض ممطرنا والـ A فتعرض في الشيء أي تحول + +18 +00:01:37,620 --> 00:01:44,800 +وتمنع ويسمى السحاب بالعارض لمنعه أثر الشمس وشعاعها + +19 +00:01:44,800 --> 00:01:51,320 +معنى العوارض في الإصطلاح هي أحوال تطرق على الإنسان + +20 +00:01:51,320 --> 00:01:57,760 +المكلف في أهليته دون أن تكون ملازمة له هذه الأمور + +21 +00:01:57,760 --> 00:02:03,640 +التي لها تأثيرفي تغيير الأحكام عوارض لمنعها + +22 +00:02:03,640 --> 00:02:09,660 +الأحكام التي تتعلق بأهلية الوجوب وأهلية الأداء عن + +23 +00:02:09,660 --> 00:02:15,760 +الثبوت بالنسبة لتأثير العوارض طبعا ما سبق أن + +24 +00:02:15,760 --> 00:02:20,380 +تحدثنا عنه في الأهلية هو بناء على الحالة الطبيعية + +25 +00:02:20,380 --> 00:02:24,500 +التي يكون فيها الشخص سليما غير أنه قد يترأ على + +26 +00:02:24,500 --> 00:02:28,900 +الأشخاص عوارض وهذه العوارضذات تأثير على أهلية + +27 +00:02:28,900 --> 00:02:33,520 +الإنسان فإما أن تكون مزيلة لأهلية الوجوب كما في + +28 +00:02:33,520 --> 00:02:39,300 +الموت فالموت عاجزة منافق لأهلية أحكام الدنيا من كل + +29 +00:02:39,300 --> 00:02:44,700 +ما فيه تكليف وهذه العوارض تزيل أحيانا أهلية الأداء + +30 +00:02:44,700 --> 00:02:48,760 +كحالتين النوم والاغماء وأحيانا أخرى لا تزيل هذه + +31 +00:02:48,760 --> 00:02:53,140 +العوارض لأهلية الوجوب ولا أهلية الأداء لكنها تغير + +32 +00:02:53,140 --> 00:02:58,700 +بعض الأحكام مع باقائهممع بقاء أهلية الوجوه و أهلية + +33 +00:02:58,700 --> 00:03:03,240 +الأداء كما في حالتي السفر والجهل في دار الحرب كل + +34 +00:03:03,240 --> 00:03:09,400 +هذا طبعا هيجي الحديث عن التفصيل فيه بالنسبة لعوارض + +35 +00:03:09,400 --> 00:03:15,600 +علماء الوصول يقسم العوارض إلى قسمين رئيسيين هما + +36 +00:03:15,600 --> 00:03:20,260 +العوارض السماوية و العوارض المكتسبة خلينا نتحدث + +37 +00:03:20,260 --> 00:03:27,830 +اليوم عن العوارض السماويةو قبل أن نبدأ بهذه + +38 +00:03:27,830 --> 00:03:34,710 +العوارض خلّينا نقف على حقيقة العوارض السماوية طبعا + +39 +00:03:34,710 --> 00:03:39,530 +العوارض السماوية ما ثبت من قبل صاحب الشرع بدون + +40 +00:03:39,530 --> 00:03:46,170 +اختيار للشخص فيه أي الإنسان ليس له كسب ولا اختيار + +41 +00:03:46,170 --> 00:03:52,730 +في هذا الأمر طبعا هذه العوارض لا دخلة للإنسان فيها + +42 +00:03:53,300 --> 00:03:57,020 +ونصبت إلى السماء لنزولها من السماء وهي خارجة عن + +43 +00:03:57,020 --> 00:04:00,840 +قدرة الإنسان وقدم العلماء الحديث عن العوارض + +44 +00:04:00,840 --> 00:04:05,280 +السماوية على العوارض المكتسبة ليش؟ لأنها أظهر في + +45 +00:04:05,280 --> 00:04:09,920 +العارضية لخروجها عن اختيار الإنسان وأشد تأثيرا في + +46 +00:04:09,920 --> 00:04:16,880 +الأحكام المكتسبة طبعا بالنسبة للعوارض السماوية + +47 +00:04:16,880 --> 00:04:24,220 +قسموها العلماء إلى 11 قسمالصغر والجنون والعته + +48 +00:04:24,220 --> 00:04:29,120 +والنسيان والنوم والاغماء والرق اللي مش هنتحدث عنه + +49 +00:04:29,120 --> 00:04:35,560 +والمرض والحيد والنفس والموت طبعا قبل بنبدأ بالحديث + +50 +00:04:35,560 --> 00:04:40,560 +عن هذه العوارض كل عارض من هذه العوارض بدك تعمل + +51 +00:04:40,560 --> 00:04:46,620 +حسابك أنك بدك تعمليله صفحة لوحدها و تبدأ بتلخيص كل + +52 +00:04:46,620 --> 00:04:52,120 +عارض لوحده بإيدك بقلمك تمسك القلم و بتبدأالصغر + +53 +00:04:52,120 --> 00:04:56,120 +مثلا تعريفه و الأحكام اللي متعلقة فيه تأثيره على + +54 +00:04:56,120 --> 00:05:01,440 +ال .. ال .. طبعا الأحكام التكلفية أو عقود البيع + +55 +00:05:01,440 --> 00:05:06,420 +مدى تأثيره على أهلية الوجوب أهلية الأداء على حسب + +56 +00:05:06,420 --> 00:05:11,400 +يعني ما احنا هنشرح عنه معناه ذلك انك انت الآن + +57 +00:05:11,400 --> 00:05:16,100 +بالنسبة للأعراض العوارض السماوية العشر عوارض هدول + +58 +00:05:16,100 --> 00:05:19,220 +لإنه احنا هنستثنى الرق لإنه مافيش إله داعي لحديث + +59 +00:05:19,220 --> 00:05:23,950 +عنه اليوم لإنه مش موجودهيكون عندك عشر ورقات من + +60 +00:05:23,950 --> 00:05:27,570 +دفتر أبو أربعين، أدول العشر ورقات فيهم تلخيص + +61 +00:05:27,570 --> 00:05:32,930 +للعوارض السماوية زي ما هنحكي كل عارض إيش هيتعلق + +62 +00:05:32,930 --> 00:05:37,630 +فيه و هيك بتكون سهلت عليك و يسرطلك الأمر في + +63 +00:05:37,630 --> 00:05:43,930 +الدراسة، للاختبار، إن هي إيه بإذن الله تعالى نبدأ + +64 +00:05:43,930 --> 00:05:48,370 +الحديث عن العارض الأول وهو الصغر، زي ما اتفقنا + +65 +00:05:48,370 --> 00:05:52,620 +بدنا نبدأ بتعريف الصغرطبعا زي ما انتوا شايفين + +66 +00:05:52,620 --> 00:05:58,160 +التعريف لازم يكون صغير مش كبير هذا بيسموه العلماء + +67 +00:05:58,160 --> 00:06:05,480 +تعريف بالرسم أو التعريف بالأثر بالنسبالي الصغر هو + +68 +00:06:05,480 --> 00:06:10,760 +المرحلة اللتي تسبق طور البلوغ سواء كان هذا البلوغ + +69 +00:06:10,760 --> 00:06:14,640 +طبيعيا عن طريق العلامات الطبيعية كخروج المنو و + +70 +00:06:14,640 --> 00:06:22,050 +انبات الشعر أو الحمل أو نزول الدمالحيد او تقديريا + +71 +00:06:22,050 --> 00:06:28,410 +اي عن طريق تقدير العمر كتقديره بخمسة عشرة عامة اذا + +72 +00:06:28,410 --> 00:06:33,150 +البلوغ هو عبارة عن مرحلة تسبق طور البلوغ الصغر + +73 +00:06:33,150 --> 00:06:38,770 +اسفة الصغر هو عبارة عن مرحلة تسبق طور البلوغ طبعا + +74 +00:06:38,770 --> 00:06:43,170 +ايش البلوغ؟ البلوغ ده ممكن يكون طبيعي بظهور + +75 +00:06:43,170 --> 00:06:48,830 +العلامات الطبيعية على ال .. ال .. الولد او البنت + +76 +00:06:49,030 --> 00:06:55,330 +حسب يعني المعداد أو تقديريا يعني مافيش عندي علامات + +77 +00:06:55,330 --> 00:07:00,530 +طبيعية بروح بعمل تقدير عن طريق تقدير العمر و أحدده + +78 +00:07:00,530 --> 00:07:08,330 +العلماء بخمسة عشرة عامة نيجي لمراحل الصغر و هان + +79 +00:07:08,330 --> 00:07:12,930 +احنا الحديث هيكون المرحلة الأولى ما قبل التمييز و + +80 +00:07:12,930 --> 00:07:16,630 +المرحلة التانية اللي هي مرحلة التمييز طبعا كل + +81 +00:07:16,630 --> 00:07:22,710 +مرحلة من هذه المراحللها أحكام تتعلق بها مرحلة ما + +82 +00:07:22,710 --> 00:07:28,790 +قبل التمييز تبدأ هذه المرحلة من حين مولد الصبي إلى + +83 +00:07:28,790 --> 00:07:34,410 +أن يقارب سن السابعة يعني من حين الولادة لسن سبعة + +84 +00:07:34,410 --> 00:07:37,930 +سنين في هذه المرحلة هذه الأحكام اللي بتتعلق بيها + +85 +00:07:37,930 --> 00:07:42,850 +تسقط عنه العبادات لا يصح تكليفه لفقده العقل + +86 +00:07:42,850 --> 00:07:48,460 +والتمييز معهلكن يؤخذ بضمان الأفعال في المطلفات + +87 +00:07:48,460 --> 00:07:53,460 +وحكينا مثال يمكن أنتم متذكرينه حكيناه مرة في + +88 +00:07:53,460 --> 00:08:00,320 +المحادرات السابقة أن جدتنا زمان إيش كانت يقول أو + +89 +00:08:00,320 --> 00:08:04,520 +جدادنا الكبار إيش كانوا يحكوا لصغير ورا إيش ورا + +90 +00:08:04,520 --> 00:08:09,940 +كبير هي معناة ضمان المطلفات مش معقول الصبي يطلف + +91 +00:08:09,940 --> 00:08:17,730 +مال الغيروالتلف هذا يكون مسامح فيه، لأ هذا لابد أن + +92 +00:08:17,730 --> 00:08:21,350 +يأخذ بالضمان حتى يحافظ على ممتلكات الآخرين يعني + +93 +00:08:21,350 --> 00:08:25,210 +حكمة المشرع من ضمان المتلفات اللي هي إيه المحافظة + +94 +00:08:25,210 --> 00:08:30,040 +على ضمان الآخرين وإذا بتتذكروا لما حكيتلكمالولد + +95 +00:08:30,040 --> 00:08:35,460 +اللي أمه ماخدها معاها على محل .. و هذا المحل بيبيع + +96 +00:08:35,460 --> 00:08:39,940 +فناجين و كبيات و إزاز و كذا و راح كصرف من الفناجين + +97 +00:08:39,940 --> 00:08:43,980 +هذه بنفع أمه تقول لصاحب المحل أسفة و تمشي و لا + +98 +00:08:43,980 --> 00:08:49,500 +بتدفع بدل المطلف هذا؟ هيدفعها بدل المطلف هذا المرة + +99 +00:08:49,500 --> 00:08:54,300 +الجاية مش هتاخد ابنها معاها أو ه .. هي تمسكه في + +100 +00:08:54,300 --> 00:08:58,490 +إيدها و مش هتسيبهيتلف أموال الآخرين وان هنا تظهر + +101 +00:08:58,490 --> 00:09:02,550 +حكمة المشرع في المحافظة على الأموال الآخرين بهذا + +102 +00:09:02,550 --> 00:09:07,050 +الضمان إذن مرحلة ما قبل التمييز تبدأ من حين + +103 +00:09:07,050 --> 00:09:12,350 +الولادة إلى سن السابعة هذه المرحلة تسقط عنه + +104 +00:09:12,350 --> 00:09:17,150 +العبادات ولا يصح تكلفه لفقده العقل والتمييز معا + +105 +00:09:17,150 --> 00:09:22,950 +ولكن يؤخذ بضمان الأفعال في المطلفات فزي ما قلنا لو + +106 +00:09:22,950 --> 00:09:28,100 +أطلف مال غيره ضمنأما بالنسبة لأقواله فلا تعتبر + +107 +00:09:28,100 --> 00:09:33,400 +شرعا، يعني لو باع، لو رهن، لو أجر، كل هذه أقواله + +108 +00:09:33,400 --> 00:09:37,860 +باطلة وغير معتبرة، لعدم تعقل المعاني، وعلى هذا فلا + +109 +00:09:37,860 --> 00:09:43,220 +تصح لا عقوده ولا إكراراته، وحتى إن أجازها الولي في + +110 +00:09:43,220 --> 00:09:48,840 +مرحلة ما قبل التمييز، معناه هذاإنه الصغير في هذه + +111 +00:09:48,840 --> 00:09:53,580 +الحالة كالمجنون بل هو أدنى منه لأن المجنون قد يميز + +112 +00:09:53,580 --> 00:09:58,980 +وإن فقد العقل أما الصغير ففاقد للعقل والتمييز معاه + +113 +00:09:58,980 --> 00:10:05,020 +نيجي لمرحلة ثانية من مراحل التمييز تبدأ طبعا هان + +114 +00:10:05,020 --> 00:10:09,900 +من حيث انتهينا من المرحلة الأولى وهي سن السابعة + +115 +00:10:09,900 --> 00:10:15,800 +وتنتهي بالبلوغفي هذه المرحلة يرقى الطفل بيكبر تبدو + +116 +00:10:15,800 --> 00:10:20,980 +عليه آثار العقل أصبح صاحب أهلية وكان ينبغي أن يثبت + +117 +00:10:20,980 --> 00:10:26,500 +في حقه وجوب الأداء بحسب وجود الأهلية لكن الشارع + +118 +00:10:26,500 --> 00:10:31,340 +اعتبره اعتبر صغره عذرا لأن الأهلية التي نالها + +119 +00:10:31,340 --> 00:10:37,040 +أهلية قاسرة لقصور عقله بسبب بقاء صغره وعدم بلوغ + +120 +00:10:37,040 --> 00:10:41,840 +عقله غير الاعتدال هنا الأحكام اللي بتتعلق فيهتسقط + +121 +00:10:41,840 --> 00:10:45,500 +عن المميز ما يحتمل السقوط من حقوق الله تعالى + +122 +00:10:45,500 --> 00:10:50,260 +كالصوم والصلاة وسائر الإعبادات فإنها محتملة للسقوط + +123 +00:10:50,260 --> 00:10:54,980 +عند وجود الأعذار أصلا يعني ولا يسقط عنه ما لا + +124 +00:10:54,980 --> 00:11:00,620 +يحتمل السقوط كعقيدة الإيمان لأنه فرض دائم لا يقبل + +125 +00:11:00,620 --> 00:11:05,680 +السقوط لأن الله تعالى إله دائم منزه عن الزوال + +126 +00:11:05,680 --> 00:11:12,330 +والتغيير فأصبح وجوب التوحيد دائما بدوامالألوهية و + +127 +00:11:12,330 --> 00:11:17,010 +لو سألناكوا ما سبب اختيار الفقهاء لأن تكون سن + +128 +00:11:17,010 --> 00:11:20,930 +السابعة بداية مرحلة التمييز عند الصغير على طول + +129 +00:11:20,930 --> 00:11:25,310 +الجواب بيكون اه صح يا أسماء bravo عليك يا صفية + +130 +00:11:25,310 --> 00:11:30,580 +دايما بتسبق ايه؟في الإجابة وهو قول النبي صلى الله + +131 +00:11:30,580 --> 00:11:34,460 +عليه وسلم قروصوا بيانكم بالصلاة لسبع سنين واضربوهم + +132 +00:11:34,460 --> 00:11:38,720 +عليها لعشر سنين وفرقوا بينهم في المضاجع فكان هذا + +133 +00:11:38,720 --> 00:11:44,520 +دليل على اختيار الفقهة أو الوقوف على سن السابع وهي + +134 +00:11:44,520 --> 00:11:49,320 +بداية لمرحلة التمييز عند الصغير طبعا هنا لا يخفى + +135 +00:11:49,320 --> 00:11:51,700 +عليكم أن الأمر بالصلاة في حديث الرسول صلى الله + +136 +00:11:51,700 --> 00:11:56,540 +عليه وسلمفي هذه السنة من العمر بمثابة الأمر + +137 +00:11:56,540 --> 00:12:01,180 +بالتمرين والتدريب للصبي لأجل أن يعتاد أداء الصلاة + +138 +00:12:01,180 --> 00:12:07,700 +عند غلوغه واكتمال عقله وسمي الصبي مميزا لأنه أصبح + +139 +00:12:07,700 --> 00:12:12,160 +الأن يميز بين الأقوال والأفعال بيفرق عن المرحلة + +140 +00:12:12,160 --> 00:12:16,060 +قبل التمييز قبل السن السابع ماكانش يفهم ولا كان + +141 +00:12:16,060 --> 00:12:21,620 +يميز لكن هنايعني بدأ عقله ينمو و يكبر فأصبح يميز + +142 +00:12:21,620 --> 00:12:26,820 +بين الأقوال و الأفعال و بين الخير و الشر و بين + +143 +00:12:26,820 --> 00:12:33,640 +الجيد و الراضي هذا الحديث كان عن مرح عن الصغر إذا + +144 +00:12:33,640 --> 00:12:42,310 +لو سألتك سؤالعرفي الصغر واتحدثي عن المراحل التي + +145 +00:12:42,310 --> 00:12:48,150 +يمروا بها الصغير وما يتعلق بها من أحكام إجابتك + +146 +00:12:48,150 --> 00:12:52,730 +بتكون الكلام اللي حكيناه سابقا أكتفي بهذا القدر في + +147 +00:12:52,730 --> 00:12:57,090 +هذه المحاضرة على أن أستكمل باقي العوارض السماوية + +148 +00:12:57,090 --> 00:13:00,950 +في المحاضرة القادمة بإذن الله و السلام عليكم و + +149 +00:13:00,950 --> 00:13:02,570 +رحمة الله وبركاته + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/-3skSIY6DA0.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/-3skSIY6DA0.srt new file mode 100644 index 0000000000000000000000000000000000000000..9f6fb62ad5198149e769a10b796e3be9bcd7d953 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/-3skSIY6DA0.srt @@ -0,0 +1,2579 @@ +1 +00:00:21,110 --> 00:00:24,130 +بسم الله الرحمن الرحيم Chapter جديد و احنا ممكن + +2 +00:00:24,130 --> 00:00:27,410 +اتحدثنا سابقا أن عنوان الـ course تبعنا + +3 +00:00:27,410 --> 00:00:30,330 +essential medical microbiology كل مدرستناها في + +4 +00:00:30,330 --> 00:00:33,110 +السابق هو essential و اليوم بنتكلم عن الـ medical + +5 +00:00:33,110 --> 00:00:37,130 +microbiology هنبدأ في عنوان ضخم اسمه biogenic + +6 +00:00:37,130 --> 00:00:44,410 +cocci وهي المكورات المقيحة بمعنى أنه بيصدر عنها أو + +7 +00:00:44,410 --> 00:00:47,590 +عن الـ infection تبعتها قيح أو اللي احنا بسميه صديد + +8 +00:00:47,590 --> 00:00:56,270 +فهي biogenic معناها قيحية أو صديدية الصورة + +9 +00:00:56,270 --> 00:01:00,390 +اللي أمامكم عبارة عن تشكيلة من الكوكساي اللي + +10 +00:01:00,390 --> 00:01:06,510 +موجودة اللون الأزرق أو الـ violet اللي واضح هنا هي + +11 +00:01:06,510 --> 00:01:10,850 +Staphylococcus واضحة إنها grape-like clusters الـ + +12 +00:01:10,850 --> 00:01:14,490 +arrangement تبعتها اللي أسفل منها هي عبارة عن + +13 +00:01:14,490 --> 00:01:18,730 +Staphylococci لاحظوا أنه الـ chains very long طويلة + +14 +00:01:18,730 --> 00:01:23,720 +جدا السلاسل اللي على اليسار في إندها نة Diplococci + +15 +00:01:23,720 --> 00:01:28,800 +لكن الشريحة مش pure زي الأولانية و التانية و في + +16 +00:01:28,800 --> 00:01:31,760 +إيها خلفية و الخلفية هذه حقيقة اللون الأحمر اللي + +17 +00:01:31,760 --> 00:01:36,160 +شايفينه هذا و اللي جواته أنوية هي عبارة عن pus + +18 +00:01:36,160 --> 00:01:40,420 +cells خلايا صديدية، إيش هي الـ pus cells؟ هي عبارة + +19 +00:01:40,420 --> 00:01:44,440 +عن phagocytes، طب إيش الـ phagocytes؟ هي neutral + +20 +00:01:44,440 --> 00:01:50,520 +fields النيتروفيلز الاسم الثاني polymorphonuclear + +21 +00:01:50,520 --> 00:01:55,340 +cells كلمة polymorphonuclear nuclear يعني أنوية و + +22 +00:01:55,340 --> 00:02:00,760 +لا لأ polymorpho يعني متعددة الأشكال الأنوية طلعوا + +23 +00:02:00,760 --> 00:02:08,560 +الأنوية تبعتها تمام بالمناسبة + +24 +00:02:08,560 --> 00:02:14,340 +إيش لونها كل خلايا الـ human لو صبغناها gram + +25 +00:02:14,340 --> 00:02:19,160 +negative يعني خلايانا احنا gram negative يعني بقى + +26 +00:02:19,160 --> 00:02:25,200 +بتاخد اللون الأحمر تاخد اللون الأحمر إذا اللي + +27 +00:02:25,200 --> 00:02:30,900 +بنطلع من اللوحة دي معلومتان صغار أن البكتيريا ممكن + +28 +00:02:30,900 --> 00:02:35,320 +نشوفها في عينات مباشرة و أنا المحاضرة الماضية + +29 +00:02:35,320 --> 00:02:38,520 +اتشرحت قلت أن ممكن ناخد عينات CSF ونعملها + +30 +00:02:38,520 --> 00:02:42,660 +centrifugation ونعمل staining إليها مباشرة وبالتالي + +31 +00:02:42,660 --> 00:02:46,960 +هشوف بكتيريا إذا موجودة و هشوف أيضا pus cells إذا + +32 +00:02:46,960 --> 00:02:52,740 +موجودة هذا الترتيب تبع الخلايا هنا هو عبارة عن + +33 +00:02:52,740 --> 00:02:58,220 +Diplococci Diplococci يعني two cocci بالمناسبة + +34 +00:02:58,220 --> 00:03:02,880 +الـ Diplococci تصنف عليها streptococci يعني كل + +35 +00:03:02,880 --> 00:03:07,000 +اللوحة streptococci معالمين مع الـ clusters اللي + +36 +00:03:07,000 --> 00:03:10,340 +على اليمين اللي هي staphylococci + +37 +00:03:13,840 --> 00:03:18,620 +هذه كمان صورة بنرجع فيها شوية، لحظة الصورة بتوضح + +38 +00:03:18,620 --> 00:03:22,600 +لنا stuff، العنوان تبع الـ stuff الكوكاية، لحظة + +39 +00:03:22,600 --> 00:03:28,260 +أنها موجودة على background خضرة وفيها ثقوب، هي + +40 +00:03:28,260 --> 00:03:30,980 +عبارة عن membrane filter، الثقوب هذه عبارة عن + +41 +00:03:30,980 --> 00:03:32,900 +membrane filter، فاكرين الـ membrane filter اللي + +42 +00:03:32,900 --> 00:03:36,880 +استخدمناه في عملية التنقية، في الـ sterilization، + +43 +00:03:36,880 --> 00:03:40,890 +في الـ physical removal من الـ micro organism لما + +44 +00:03:40,890 --> 00:03:45,470 +قلنا أن المسامات بتكون أصغر من حجم البكتيريا، مش + +45 +00:03:45,470 --> 00:03:49,330 +هذه الفكرة اللي أنا بدي أوضحها، بدي أوضح أن شكل + +46 +00:03:49,330 --> 00:03:53,530 +الـ Staphylococci بيعطيني إيحاء بأن الإنقسامات + +47 +00:03:53,530 --> 00:03:58,910 +بتكون في كل الاتجاهات ممكن في هذا البعد، في هذا + +48 +00:03:58,910 --> 00:04:03,570 +البعد، البعد الأسفل، اليمين إلى اليسار بينما لو + +49 +00:04:03,570 --> 00:04:07,630 +البكتيريا Streptococci هتكون الانقسام في اتجاه + +50 +00:04:07,630 --> 00:04:12,390 +واحد ما الذي يحدد طريقة الانقسام؟ أيضا هو الـ + +51 +00:04:12,390 --> 00:04:17,230 +genetic makeup، يعني التكوين الوراثي للبكتيريا + +52 +00:04:19,680 --> 00:04:24,220 +morphology and identification للاستثمار اللي كوكا، + +53 +00:04:24,220 --> 00:04:27,360 +they are gram positive و هذه حقيقة خلاص هي ثابتة + +54 +00:04:27,360 --> 00:04:31,980 +الآن، يعني ما فيش داعي نضل نكررها، بس أساسية في + +55 +00:04:31,980 --> 00:04:35,960 +التعرف، فكنت دي في أنا أيروب، يعني أنا مجانا نميها + +56 +00:04:35,960 --> 00:04:40,220 +هوائيا ولا هوائيا، grape-like clusters كعناقيد + +57 +00:04:40,220 --> 00:04:45,630 +لاقينا بشكل البكتيريا تحت الـ microscope كتاليز + +58 +00:04:45,630 --> 00:04:52,550 +بوستف وهذه صفة محورية إيه صفة محورية يعني احنا + +59 +00:04:52,550 --> 00:05:00,490 +هندرس تحت الـ biogenic cocci to two genuses من + +60 +00:05:00,490 --> 00:05:03,030 +الجرام بوستف في كوكساية الاستفيل الكوكاية + +61 +00:05:03,030 --> 00:05:05,990 +والاستريبت الكوكاية يعني في يوم من الأيام لو أنا + +62 +00:05:05,990 --> 00:05:08,850 +عزلت جرام بوستف في كوكساية فهي واحدة من إثنتين + +63 +00:05:12,020 --> 00:05:15,700 +الصفر اللي بيبقى مميزة بينهم الكتاليز الستفيل + +64 +00:05:15,700 --> 00:05:18,600 +الكوكاية كتاليز بوستف بينما الستفيل الكوكاية + +65 +00:05:18,600 --> 00:05:21,900 +كتاليز نيجاتيف هذه نقطة محورية كمان مرة في الـ + +66 +00:05:21,900 --> 00:05:26,460 +diagnosis major component of normal flora of the + +67 +00:05:26,460 --> 00:05:30,440 +nose and skin وكلمة major component يعني هي جزء + +68 +00:05:30,440 --> 00:05:34,080 +أساسي أو مكون أساسي من الـ normal flora في الأنفة + +69 +00:05:34,080 --> 00:05:38,260 +اللي هو الـ الجلد المعلومات هذه مهمة عشان نحن نحن + +70 +00:05:38,260 --> 00:05:43,780 +نرجعلها أنها skin و nose هذه أيضا صورة بتوضح أن الـ + +71 +00:05:43,780 --> 00:05:46,580 +... الـ ... اليسار الـ gram-positive-cocci مأخودة من + +72 +00:05:46,580 --> 00:05:49,800 +culture، يعني من مستعمرة، تمام؟ وبتاع الـ ... الـ + +73 +00:05:49,800 --> 00:05:52,800 +background تبعتها واضحة، ما فيش فيها أي شيء، بينما + +74 +00:05:52,800 --> 00:05:57,660 +الأخرى هذي، إيش هتحب تكتب عليها؟ بس، دلوقت + +75 +00:05:57,660 --> 00:06:03,320 +أنها مأخودة من عينة فيها صديد وصبغناها، الآن هذا + +76 +00:06:03,320 --> 00:06:09,360 +المشكلة هل نستطيع بدقة و حرافية أن نميز الاستفادة + +77 +00:06:09,360 --> 00:06:12,240 +من الكوكاية عن الاسترابات من الكوكاية من خلال + +78 +00:06:12,240 --> 00:06:14,560 +الشكل اللي احنا بقوله هذيك grape-like clusters و + +79 +00:06:14,560 --> 00:06:19,880 +التانية سلاسة لكن في واقع الأمر لو خدنا عنقود عينة + +80 +00:06:19,880 --> 00:06:29,110 +و ضربناها في الحقيق هيتفتت إلى حبات منفصلة أو حبتين + +81 +00:06:29,110 --> 00:06:32,550 +أو حبتين ييجي في جنب حبتين خدوا بالكوا الأشكال + +82 +00:06:32,550 --> 00:06:35,890 +اللي أنا حاكيتهم مختلفين تماما حبة single cell + +83 +00:06:35,890 --> 00:06:40,290 +حبتين Diplococci تنتهي إثنتين سلسلة Streptococci + +84 +00:06:40,290 --> 00:06:44,490 +الكوكاي أربع لو كانوا تكوّموا في بعض Diplococci طب + +85 +00:06:44,490 --> 00:06:50,010 +أنا كيف بدي أعرف أن المدولة عنقود على فرفط انعقد + +86 +00:06:50,010 --> 00:06:56,400 +الحبات تبعتها راحت ولا هو واقع الأمر هيك فببدأ أستخدم + +87 +00:06:56,400 --> 00:07:00,620 +مين؟ الكتاليز إذا you cannot identify أو + +88 +00:07:00,620 --> 00:07:03,740 +differentiate Staphylococci from streptococci with + +89 +00:07:03,740 --> 00:07:07,080 +certainty unless you perform the test اللي اسمه + +90 +00:07:07,080 --> 00:07:12,180 +كتاليز و الكتاليز من أسهل الفحوصات بندرجها من ضمن + +91 +00:07:12,180 --> 00:07:15,880 +الـ one minute test one minute test يعني يعني + +92 +00:07:15,880 --> 00:07:21,220 +بيستغرقش معانا دقيقة حتى هذه الصورة اللي أمامنا + +93 +00:07:21,220 --> 00:07:24,440 +بتوضح لنا اللي هي فكرة الفحص وهو عبارة عن قطرة من + +94 +00:07:24,440 --> 00:07:27,240 +hydrogen peroxide 3% بنضيف عليها الـ + +95 +00:07:27,240 --> 00:07:31,260 +micro organism إذا الـ micro organism بيحتوي على + +96 +00:07:31,260 --> 00:07:36,920 +الكتاليز هيحلل الـ hydrogen peroxide و oxygen الآن + +97 +00:07:36,920 --> 00:07:40,700 +الـ oxygen تكونه ضمن الـ water اللي هو الـ hydrogen + +98 +00:07:40,700 --> 00:07:44,980 +peroxide فبتطلع بشكل فقاعة خذوا بالكم من النقطة + +99 +00:07:44,980 --> 00:07:48,160 +كمان شوية هروح على step 2 الكوكاي أو مش كمان شوية + +100 +00:07:48,160 --> 00:07:52,660 +المحاضرة اللي قادمة فحلاجيها catalase negative لكن + +101 +00:07:52,660 --> 00:07:57,200 +حلاجيها facultative anaerobic facultative + +102 +00:07:57,200 --> 00:08:03,440 +anaerobic مؤشر على أنه عندها mechanism by which + +103 +00:08:03,440 --> 00:08:11,220 +they can defend themselves against oxygen مثلا الـ + +104 +00:08:11,220 --> 00:08:16,100 +catalase هنا هو المصب هو اللي حمانه من آثار الـ + +105 +00:08:16,100 --> 00:08:20,080 +hydrogen peroxide طب هناك مين بيحميها من الهيدرولين + +106 +00:08:20,080 --> 00:08:23,540 +بيروكسايد فاكرين يقولنا الأن دي متباعتنا معتمد على + +107 +00:08:23,540 --> 00:08:30,980 +super oxide dismutase و catalase أو peroxidase الآن الـ + +108 +00:08:30,980 --> 00:08:34,240 +Streptococcus فيها peroxidase ما فيش فيها catalase + +109 +00:08:34,240 --> 00:08:35,860 +عشان يسمينا catalase negative + +110 +00:08:39,900 --> 00:08:42,620 +قبل ما ندخل في بعض الـ Genesis و الـ immunity لـ الـ + +111 +00:08:42,620 --> 00:08:46,600 +Staphylococcus species نظهر على الـ cell wall و + +112 +00:08:46,600 --> 00:08:49,000 +نتذكر مع بعض بعض الـ components اللي موجودة في الـ + +113 +00:08:49,000 --> 00:08:52,920 +cell wall capsule or polysaccharide slime layer + +114 +00:08:52,920 --> 00:09:02,320 +ليش or؟ كلمة or معناها أن بعض السلالات تحتوي عليها + +115 +00:09:02,320 --> 00:09:06,640 +و سلالة أخرى تحتوي عليها كلمة سلالة بدنا نفهمها + +116 +00:09:06,640 --> 00:09:10,220 +اليوم لأن هو كثير و كثير مهمة أنتم طالبات سنة + +117 +00:09:10,220 --> 00:09:13,320 +ثانية بيتعامل معاكم بهذا الأنواع أنتم طالبات سنة + +118 +00:09:13,320 --> 00:09:16,780 +ثانية أنتم كلكم واحد لكن الـ ... الـ ... الـ ... الـ + +119 +00:09:16,780 --> 00:09:17,160 +الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ + +120 +00:09:17,160 --> 00:09:18,420 +... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... + +121 +00:09:18,420 --> 00:09:18,860 +... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... + +122 +00:09:18,860 --> 00:09:19,580 +... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... + +123 +00:09:19,580 --> 00:09:19,660 +الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ + +124 +00:09:19,660 --> 00:09:20,440 +... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... الـ ... + +125 +00:09:20,440 --> 00:09:29,320 +... الـ ... الـ ... الـ ... الـ ... + +126 +00:09:29,320 --> 00:09:30,920 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +127 +00:09:30,920 --> 00:09:34,380 +.. ال .. ال + +128 +00:09:36,580 --> 00:09:39,820 +بس لازم يطر على بالك مجموعة زي اللي رحجته طلاب + +129 +00:09:39,820 --> 00:09:43,760 +سنة تانية مع الحاجة إن ستافيلو كوكاس أورس ليش + +130 +00:09:43,760 --> 00:09:48,520 +فيها strains مختلفة هي species لكن فيها strains + +131 +00:09:48,520 --> 00:09:53,180 +مختلفة قد يحتوي ال strain على توكسين X و ال strain + +132 +00:09:53,180 --> 00:09:56,660 +بتاعي ليه يحتوي على هذا التوكسين لكن لما أنا بدي + +133 +00:09:56,660 --> 00:10:00,520 +بدرسهم إلكوا و بحطوكوا كل ال toxins اللي في كل ال + +134 +00:10:00,520 --> 00:10:05,850 +strains ولا لأ؟ طيب بيبتدش lie can layer إيه أهمية + +135 +00:10:05,850 --> 00:10:11,030 +بالنسبة للبروتين نفسه اللي مانوش أهمية؟ إيش أهميته في + +136 +00:10:11,030 --> 00:10:14,810 +البعض نفسه؟ لما اتحدثنا عن ال gram negative بس ال + +137 +00:10:14,810 --> 00:10:20,690 +lie، اتحدثت أنا عن حاجة اسمها gram negative ليبو + +138 +00:10:20,690 --> 00:10:24,210 +بوليساكرايد و اتحدثنا عن مصائب ليبو بوليساكرايد و + +139 +00:10:24,210 --> 00:10:29,060 +سئلنا سؤال بريء، هل هناك في الإجراء مبسطة في الـ + +140 +00:10:29,060 --> 00:10:32,760 +counter part اللي اللي يقول سكراي و الجواب نعم و + +141 +00:10:32,760 --> 00:10:37,600 +اللي هو اللي بيقلده is like إذن إيه أهمية في + +142 +00:10:37,600 --> 00:10:41,100 +الفعالية الجنسية للستافيلو كوكاس؟ في هذا التيكويك + +143 +00:10:41,100 --> 00:10:44,840 +أسد كنا قلنا أيضا إله أهمية في الفعالية الجنسية و + +144 +00:10:44,840 --> 00:10:50,560 +البروتين E اللي كان يمسك ال Antibody كنا قلنا + +145 +00:10:50,560 --> 00:10:53,700 +عملينه two antigen binding site و من تحت اللي هو + +146 +00:10:53,700 --> 00:10:56,700 +ال Fc portion كان بدل من ارتبط زي هيك في + +147 +00:10:56,700 --> 00:10:59,480 +البكتيريا، البكتيريا تربطه من تحت، هذا البروتين E + +148 +00:10:59,480 --> 00:11:02,800 +كنا قلنا عنه من أعظم ال viral instructors في + +149 +00:11:02,800 --> 00:11:08,610 +الستافيلو كوكاس فينا طبعا الـ cell membrane ده + +150 +00:11:08,610 --> 00:11:11,790 +component طبيعي موجودة، ال clamping factor أو ما + +151 +00:11:11,790 --> 00:11:17,190 +يسمى بال coagulase و مارو معانا ذكره اللي بيحول ال + +152 +00:11:17,190 --> 00:11:23,000 +fibrinogen إلى fibrin هذا الشكل دراساناه وشوفناه هو + +153 +00:11:23,000 --> 00:11:26,260 +ال protein A بقولنا إنه بيرتبط ال .. ال .. هي ال + +154 +00:11:26,260 --> 00:11:29,640 +immunoglobulin و هو ال protein A بيرتبط منين؟ + +155 +00:11:29,640 --> 00:11:33,300 +بيرتبط بال Fc portion و ال Fc portion هذا مخصص + +156 +00:11:33,300 --> 00:11:36,600 +للارتباط بال macro phage و بال phagocyte so + +157 +00:11:36,600 --> 00:11:39,700 +بالتالي ال macro phage و ال phagocyte لن تجد شيء + +158 +00:11:39,700 --> 00:11:43,820 +ترتبط فيه وبالتالي لن تستطيع الانتقام للبكتيريا + +159 +00:11:43,820 --> 00:11:48,010 +ليها Staphylococcus aureus من بعض ال toxins و ال + +160 +00:11:48,010 --> 00:11:52,150 +enzymes اللي متوفرة في حضرة ال Staphylococci و + +161 +00:11:52,150 --> 00:11:56,370 +Aureus بالذات ال Catalase و يعرفنا إيش الأهمية + +162 +00:11:56,370 --> 00:11:59,550 +تبعته ال Coagulase ماعرفناش الأهمية .. أنا + +163 +00:11:59,550 --> 00:12:03,930 +قولتلكوا و نزلتكوا فيديو على الصفحة اسمه virulence + +164 +00:12:03,930 --> 00:12:08,230 +factors of staphylococcus aureus هرجعوا لحضراتكم + +165 +00:12:08,230 --> 00:12:12,790 +إنكوا تطلعوا عليه و عشر دقائق أقل من عشر دقائق + +166 +00:12:12,790 --> 00:12:18,380 +تسعة أو أشهر تمام يعني هم بتطلعوا عليهو تشوفوا ال + +167 +00:12:18,380 --> 00:12:21,120 +.. ال .. ال .. ال violence factor وقتش بيلزم + +168 +00:12:21,120 --> 00:12:25,520 +البكتيريا من دخولها لحد وصولها للدمخ و كيف ال + +169 +00:12:25,520 --> 00:12:29,320 +coagulase بيساعدها كيف بيساعدها ال .. ال .. ال .. + +170 +00:12:29,320 --> 00:12:31,780 +ال .. ال hyaluronidase على سبيل المثال كل هذه + +171 +00:12:31,780 --> 00:12:36,180 +الأشياء من .. يعني موجودة في هذا البيت catalase, + +172 +00:12:36,300 --> 00:12:40,440 +coagulase, hyaluronidase and lipase hemolysin أو + +173 +00:12:40,440 --> 00:12:44,080 +حاجة بيسموها sphingomyelinase-c و كل .. كل كنت + +174 +00:12:44,080 --> 00:12:49,620 +بتعرف هيموليسين يعني Toxin that will disrupt the + +175 +00:12:49,620 --> 00:12:51,960 +cell membrane of red blood cell و بيعملها + +176 +00:12:51,960 --> 00:12:55,260 +destruction كيف كهو؟ بيعمل destruction ل red blood + +177 +00:12:55,260 --> 00:12:59,120 +cell ل cell membrane و بدلا بيصير lysis كلمة lysis + +178 +00:12:59,120 --> 00:13:04,280 +لدم hemolysis همولايسيز تبعها هذا مؤشر أن + +179 +00:13:04,280 --> 00:13:07,700 +الستافيلو كوكاس أوريس اللي بتمتلك هذه ال toxin لو + +180 +00:13:07,700 --> 00:13:11,380 +زرعناها ع بلد أجارها بنات هتعمل hemolysis حول + +181 +00:13:11,380 --> 00:13:16,620 +المستعمرة تبعها و ال hemolysis صفة من الصفات المهمة + +182 +00:13:16,620 --> 00:13:21,100 +جدا اللي نستخدمها بالتعرف على البكتير Exfoliative + +183 +00:13:21,100 --> 00:13:27,040 +toxin أول مرة شفتوا طفل بتجشر أطفال الجيل تبعه + +184 +00:13:27,040 --> 00:13:34,210 +بتجشر، ماشفتوش؟ بتجشروا؟ أه ده طبيعي لكن لما يكون + +185 +00:13:34,210 --> 00:13:38,950 +أو بلاش أطفال راحوا على البحر و من الشمس طبعا + +186 +00:13:38,950 --> 00:13:43,710 +صاروا طبقات من الجلد يمكن نزعها من هؤلاء الأطفال + +187 +00:13:43,710 --> 00:13:47,450 +أو حتى من الأكبر ال Exfoliative toxin اللي + +188 +00:13:47,450 --> 00:13:50,590 +البكتيريا عملت infection لهذا الإنسان بصير جلده + +189 +00:13:50,590 --> 00:13:55,770 +يتقشر كما و إنه بالفعل يعني قشرة خارجي toxic shock + +190 +00:13:55,770 --> 00:14:01,700 +syndrome توكسين و بينبوسين super antigen و ال .. ال + +191 +00:14:01,700 --> 00:14:07,420 +enterotoxin أنا بتركز على آخر اتنين و أخد منهم آخر + +192 +00:14:07,420 --> 00:14:14,260 +واحد ال enterotoxin إيش معنى entero؟ معوي لأ لك + +193 +00:14:14,260 --> 00:14:18,900 +أندو entero enteric معوي و إحنا حاكيها تقولنا إنه + +194 +00:14:18,900 --> 00:14:22,720 +تصنف ال toxins بحسب ال site ال euro أو ال terro + +195 +00:14:22,720 --> 00:14:28,200 +إذا أنا مابشتل هذا؟ في ال intestine طيب اسمعوا هل + +196 +00:14:28,200 --> 00:14:31,730 +الكلام موضوع حد مبسوط عليه خالص؟ اللي هو علاقة + +197 +00:14:31,730 --> 00:14:36,110 +بالمفتول؟ أظن أن كلكم بتعرفوا المفتول و لا بتعرفوش + +198 +00:14:36,110 --> 00:14:41,510 +المفتول و لا ليهم برضه من فرنسا من غزة هذكركم + +199 +00:14:41,510 --> 00:14:44,770 +الخطوات اللي .. اللي .. المعمول بيها بالمفتول عشان + +200 +00:14:44,770 --> 00:14:50,010 +نعرف .. عشان نعرف وين .. وين الخفق ممكن يحدث إنتوا + +201 +00:14:50,010 --> 00:14:54,630 +بتاخدوا دقيق بتخلطوا بالماء و بعدين بتعملوا كُريات + +202 +00:14:54,630 --> 00:15:00,410 +عبر ضغطه في شبكة و من ثم تاخدوا هذه الكُريات و + +203 +00:15:00,410 --> 00:15:05,450 +تحطوها في وعاء مثقب و تحطوها على ماء أو whatsoever + +204 +00:15:05,450 --> 00:15:10,870 +سائل و بتغلو السائل فالسائل بخار تبقى و بدخل عبر + +205 +00:15:10,870 --> 00:15:16,970 +المفتول و بينضجه بينضجه يعني بيصل حرارته إلى حرارة + +206 +00:15:16,970 --> 00:15:20,410 +عالية جدا لو كان فيه ميكروبات من أي نوع غالبا + +207 +00:15:20,410 --> 00:15:24,830 +هتموت في العملية اللي هو ال cooking خلصنا من النقطة + +208 +00:15:24,830 --> 00:15:30,050 +دي، الآن نزلنا و كبنا، مش هيك صح الطريقة؟ بيكون كل + +209 +00:15:30,050 --> 00:15:33,830 +واحدة مفلوطة عن بعضها ولا مكتل؟ مكتل .. مكتل .. + +210 +00:15:33,830 --> 00:15:36,170 +مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. + +211 +00:15:36,170 --> 00:15:36,210 +مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. + +212 +00:15:36,210 --> 00:15:38,150 +مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. + +213 +00:15:38,150 --> 00:15:39,130 +مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. + +214 +00:15:39,130 --> 00:15:47,050 +مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. + +215 +00:15:47,050 --> 00:15:54,350 +مكتل .. مكتل .. زي المفتول اللي طبخناه قبل شوية + +216 +00:15:54,350 --> 00:15:59,490 +و أخذنا لوب و حطناه في عينا و زرعنا البكتيريا + +217 +00:15:59,490 --> 00:16:03,290 +أصابعنا هم اللوب تمام و أظافرنا هم البكتيريا + +218 +00:16:03,290 --> 00:16:07,090 +حطناهم في المفتول و زرعنا المفتول، الآن بكتيريا، + +219 +00:16:07,090 --> 00:16:12,960 +كويس؟ خدنا بالكم للنقطتين النقطة الأولى إن هذا + +220 +00:16:12,960 --> 00:16:17,520 +معقّم و أضفنا له بكتيريا واحدة من نوع واحد و ذكرت + +221 +00:16:17,520 --> 00:16:23,140 +أنا مكان البكتيريا قبل قليل في الأنف وين؟ في الجلد + +222 +00:16:23,140 --> 00:16:28,220 +فبالتالي إحنا زرعنا البكتيريا في مكان معقّم، إيش + +223 +00:16:28,220 --> 00:16:30,700 +يعني كلمة مكان معقّم؟ يعني لو مش معقّم أحسن، اه لو + +224 +00:16:30,700 --> 00:16:33,440 +مش معقّم أحسن فاكرين لما اتكلمنا عن normal + +225 +00:16:33,440 --> 00:16:37,960 +microbiota؟ اتكلمنا عن إنه من صفاتها إنها بتعمل + +226 +00:16:37,960 --> 00:16:41,580 +اتزان بينها وبين بعض، لإن في منها منافسة، بس ال + +227 +00:16:41,580 --> 00:16:45,300 +Staphylococcus aureus الآن، هل لها منافس؟ مالاش + +228 +00:16:45,300 --> 00:16:49,020 +منافس، هذا نقطة، نقطة تانية، الدقيق لما كان + +229 +00:16:49,020 --> 00:16:53,020 +uncooked، يعني غير مطبوخ، غير مطهي، ما كان فيه + +230 +00:16:53,020 --> 00:16:56,940 +لطوبة أصلا جافة، وبالتالي كان غير صارح لمين؟ لنمو + +231 +00:16:56,940 --> 00:17:01,110 +البكتيريا، الآن فيه ماء في ال .. في ال .. في الغذاء + +232 +00:17:01,110 --> 00:17:06,130 +و أصبح إيه .. ملائم و خاصة إن بعض ال nutrients + +233 +00:17:06,130 --> 00:17:10,810 +نزلت من الخلايا و صارت موجودة الآن متاحة للبكتيريا + +234 +00:17:10,810 --> 00:17:18,050 +فخلال 6 إلى 8 ساعات ابتنموا البكتيريا إلى أعداد + +235 +00:17:18,050 --> 00:17:21,990 +quorum sensing إذا وصلت البكتيريا ل quorum sensing + +236 +00:17:21,990 --> 00:17:25,150 +عشرة لقص خمسة لكل gram تبدأ تنتج ال toxin تبعها + +237 +00:17:25,150 --> 00:17:28,390 +اللي هو ال enterotoxin طبعا إحنا بنعمل .. بنعمل + +238 +00:17:28,390 --> 00:17:31,850 +مفتول كأكل شعبية، بنعملش للغدا و بس، بنعمل للغدا و + +239 +00:17:31,850 --> 00:17:36,890 +للعشاء و بنوزع الجيران و على الحباب .. المساء جينا + +240 +00:17:36,890 --> 00:17:41,010 +سخنا المفتول، بدنا نتعشى من المفتول، بعد ساعتين + +241 +00:17:41,010 --> 00:17:44,870 +صارنا مغص، vomiting اعرف إن إنت صار عندك مفتولهيتس + +242 +00:17:44,870 --> 00:17:49,410 +اللي هي food poisoning .. food poisoning .. food + +243 +00:17:49,410 --> 00:17:52,810 +poisoning .. تسموه من غذائي من ال Staphylococcus + +244 +00:17:52,810 --> 00:17:53,570 +aureus + +245 +00:17:59,010 --> 00:18:04,070 +بنقف عندها جماليا أنا قلت سخننا الأكل سخننا + +246 +00:18:04,070 --> 00:18:09,090 +الغذاء هذا بتدل على إنه لو البكتيريا ماتت مش أنا + +247 +00:18:09,090 --> 00:18:12,690 +مش هي المشكلة اللي عندي أنا عندي ال toxin و ال + +248 +00:18:12,690 --> 00:18:17,750 +toxin heat stable يعني لو سخننا و غلينا هضله زي ما + +249 +00:18:17,750 --> 00:18:23,470 +هو هو الوحيد اللي heat stable من كل ال x-toxin إيش + +250 +00:18:23,470 --> 00:18:27,860 +رأيكم؟ ال foodpoisoning enterotoxin تبع + +251 +00:18:27,860 --> 00:18:30,900 +الـ*Staphylococcus aureus* هو الوحيد اللي هو heat + +252 +00:18:30,900 --> 00:18:36,320 +stable من كل الـ exotoxins استفدنا من المعلومة هذه + +253 +00:18:36,320 --> 00:18:39,000 +مجموعة معلومة تاخدوا بالك وأن القصة مش هي اللي + +254 +00:18:39,000 --> 00:18:43,420 +بتعنيني قصة أنه أنا عندي الـ post cooking + +255 +00:18:43,420 --> 00:18:49,450 +contamination اخطر من الـ pre cookingعندي مشكلة + +256 +00:18:49,450 --> 00:18:53,950 +تانية أنه كونه الـ .. الـ .. الـ .. الـ .. الـ toxin + +257 +00:18:53,950 --> 00:18:58,210 +أتكون داخل الغذاء معناه اللي حاكي أنه أنا لم أحفظه + +258 +00:18:58,210 --> 00:19:01,670 +على temperature غير ملائمة لنمو الـ micro organism + +259 +00:19:01,670 --> 00:19:05,570 +وبالتالي هنغير نظرتنا على موضوع تخزين الأكل اللي + +260 +00:19:05,570 --> 00:19:09,050 +من ضمنها الأكل الشعبية المعروفة بالمفطول أنا ذكرت + +261 +00:19:09,050 --> 00:19:13,980 +المفطول خد بالكوا كنموذج بس هناك أغذية كثيرة قد + +262 +00:19:13,980 --> 00:19:17,340 +يحدث بها نفس الـ .. أعطاكم مثال تاني تاعة الـ cake + +263 +00:19:17,340 --> 00:19:20,640 +اللي بتفور والأشياء اللي دلوقت مرات في الاجتماعات + +264 +00:19:20,640 --> 00:19:26,940 +احنا بيجيبون لنا عجة عصير وقطعة cake أحد المرات + +265 +00:19:26,940 --> 00:19:32,480 +أنا شخصيا عندي سكر يعني فمش كتير مغرم لكن يبدو أني + +266 +00:19:32,480 --> 00:19:36,500 +كنت جاعان فأكلت .. متكلم مع اللي جان فأكلت يعني + +267 +00:19:36,500 --> 00:19:40,040 +بعد بالفعل أقل من ساعتين كل الأعراض اللي هتكلم + +268 +00:19:40,040 --> 00:19:44,000 +عنها بالنسبة لها كانت موجودة دلوقت لأنه برضه هو + +269 +00:19:44,000 --> 00:19:48,240 +food poisoning لكن + +270 +00:19:48,240 --> 00:19:51,420 +لو جينا على المستشفيات وعلى الـ medical records و + +271 +00:19:51,420 --> 00:19:55,740 +عدينا كم حالة مسجلة عندهم مشخصة رأينا food + +272 +00:19:55,740 --> 00:19:57,720 +poisoning بالـ food poisoning سأولياس هلاجيها zero + +273 +00:19:59,980 --> 00:20:03,640 +أول حاجة الـ onset اللي هي الـ disease نفسه بيستغرق + +274 +00:20:03,640 --> 00:20:08,480 +ساعات بسيطة و self-limited إيش يعني self-limited؟ + +275 +00:20:08,480 --> 00:20:12,640 +يعني لحاله والـ resolve فاحنا ما عطيناهوش أهمية كبيرة + +276 +00:20:12,640 --> 00:20:19,400 +لكن تخيلوا كتاب حضرتك 6 الصبح أو الظهر whatsoever + +277 +00:20:19,400 --> 00:20:21,860 +الموعد اللي غيرتي فيه وبعد ساعتين صار عندك اللي + +278 +00:20:21,860 --> 00:20:25,600 +هو gastroenteritis وعندك امتحان أو عندك جامعة أو + +279 +00:20:25,600 --> 00:20:30,720 +عندك lecture محاضرة أنت مش هتروح على هذا المكان في + +280 +00:20:30,720 --> 00:20:34,080 +خلال فترة الـ .. الـ .. الـ onset لن تستطيعوا بتالي + +281 +00:20:34,080 --> 00:20:38,480 +لو جينا حسبنا التكلفة الاقتصادية الناجمة عن الـ + +282 +00:20:38,480 --> 00:20:41,200 +food poisoning بستفيل الكوكس أو الهلجيهة ضخمة جدا + +283 +00:20:41,200 --> 00:20:43,940 +تخيلوا أن الوضع صار معايا أنا وغيبت أنا عن + +284 +00:20:43,940 --> 00:20:47,780 +المحاضرة هذه أو غيبت اليوم كله عن المحاضرات كم + +285 +00:20:47,780 --> 00:20:51,420 +طالبة عندي ستين طالبة ستين في مواصلاتهم اللي اجوا + +286 +00:20:51,420 --> 00:20:54,600 +فيهم والساعة الزمنية اللي استغرقوها ما بتكلم عن .. + +287 +00:20:54,600 --> 00:20:59,110 +أنا .. أنا فلوس هذا كله كان مخسر ومش مخسر، + +288 +00:20:59,110 --> 00:21:02,510 +بالإضافة إنه ممكن أنا أروح على العيادة وأدفع ثمن + +289 +00:21:02,510 --> 00:21:06,510 +علاج وهم يعطوني علاج، أنام في المستشفى، فالتكلفة + +290 +00:21:06,510 --> 00:21:10,470 +الاقتصادية لإنه بتكلم عن food والـ food غالبا هو + +291 +00:21:10,470 --> 00:21:14,130 +social occasion، إيش أنا social occasion؟ يعني وضع + +292 +00:21:14,130 --> 00:21:17,810 +اجتماعي، ما بناكلش لحالنا بالغالب، ما بناكل مع أفراد + +293 +00:21:17,810 --> 00:21:21,390 +آخرين صح أو لا؟ وبالتالي لو صار فيه food poisoning + +294 +00:21:21,390 --> 00:21:25,490 +ما نسمعش عن شخص واحد، نسمع عنه .. عن مجموعة كبيرة من + +295 +00:21:25,490 --> 00:21:29,230 +الناس، لا قدر الله احنا في .. في .. في جامعة و + +296 +00:21:29,230 --> 00:21:34,030 +الكافتيريا صار فيها غذاء ملوث ومن العشر تلاف طالبة + +297 +00:21:34,030 --> 00:21:38,470 +ثلاث تلاف كانوا وسط الكافتيريا وأكل من هذا الغذاء + +298 +00:21:39,360 --> 00:21:42,580 +أنا بحاجة كده بتكلم عن مصائب ولا لأ؟ ف الـ food + +299 +00:21:42,580 --> 00:21:46,040 +poisoning اللي .. اللي .. اللي بتسبب استفادة + +300 +00:21:46,040 --> 00:21:49,920 +الـ*Staphylococcus aureus* أولي الصحيح mild لكن بيفتح أمامنا آفاق أنه + +301 +00:21:49,920 --> 00:21:52,280 +في حد اسمه food poisoning والـ food poisoning + +302 +00:21:52,280 --> 00:21:56,020 +بيختلف عن الـ food infection الـ food infection هو + +303 +00:21:56,020 --> 00:21:58,880 +أنه أنا عايز أنور الغذاء وفيه الميكروب والميكروب + +304 +00:21:58,880 --> 00:22:03,860 +يدخل جوا ويعمل توكسين جوا بينما الـ food + +305 +00:22:03,860 --> 00:22:07,730 +poisoning الـ toxin أنصار Externally في الغذاء نفسه، + +306 +00:22:07,730 --> 00:22:10,210 +طب ما هذا نقطة كتير مهمة نجي للي جابله، هذا اللي + +307 +00:22:10,210 --> 00:22:13,710 +جابله محرج شوية اللي هو toxic shock syndrome toxin + +308 +00:22:13,710 --> 00:22:20,090 +لكن لابد أن نتطرق له بشكل جيد الفتيات عند البلوغ + +309 +00:22:20,090 --> 00:22:23,990 +بيصبح عندهم الحالة المعروفة بالدورة الشهرية، + +310 +00:22:23,990 --> 00:22:29,190 +مظبوط؟ وطبعا البنت بتخرج أو الست بتخرج إلى العمل + +311 +00:22:29,190 --> 00:22:34,710 +فبالتالي بتستخدم ما يسمى بالحفاضات النسائية الحفاضات + +312 +00:22:34,710 --> 00:22:40,130 +النسائية الآن business رائج كتير على كوكب الأرض من + +313 +00:22:40,130 --> 00:22:44,930 +الستات وبالتالي في استهلاك فالشركات تتنافس مين + +314 +00:22:44,930 --> 00:22:50,850 +ينتج أفضل أريح واللي أهم من ذلك اللي بيطول أكثر + +315 +00:22:51,670 --> 00:22:54,890 +most absorbent اللي بتمتص كمية أكبر من الدم + +316 +00:22:54,890 --> 00:22:59,350 +أذكركوا الدم ضفناه على الـ media في المعمل عشان + +317 +00:22:59,350 --> 00:23:03,030 +نعمل blood agar ليش ضفنا الـ blood؟ to enrich the + +318 +00:23:03,030 --> 00:23:07,910 +media معناه الحاجة أن الدم غني جدا بالغذاء فكمية + +319 +00:23:07,910 --> 00:23:11,050 +الدم كبيرة موجودة دقان في الـ .. في الـ .. في الـ + +320 +00:23:11,050 --> 00:23:14,990 +tampons تمام؟ والـ*Staphylococcus aureus* موجودة على + +321 +00:23:14,990 --> 00:23:19,570 +الجلد تنمو البكتيريا في الدم وتصل كوراسينسينج + +322 +00:23:19,570 --> 00:23:22,990 +وتنتج الـ super antigen تبعها اللي هو الـ toxic + +323 +00:23:22,990 --> 00:23:29,150 +shock syndrome toxin أو TSSD that will be absorbed + +324 +00:23:29,150 --> 00:23:32,590 +through the vagina of the mucous membrane. الآن + +325 +00:23:32,590 --> 00:23:36,730 +فاكرين كلمة super antigen كيف بقى بتشتغل؟ هذكركوا + +326 +00:23:39,250 --> 00:23:43,310 +اللوحة اللي قدامنا هذه بتوضح لنا أن الـ T-cell + +327 +00:23:43,310 --> 00:23:46,270 +ترتبط مع الـ macrophage الـ antigen presenting cell + +328 +00:23:46,270 --> 00:23:50,310 +specifically على الـ antigen وأنا قلت لكم أنه .. + +329 +00:23:50,310 --> 00:23:55,190 +شايفين الإشارة اللي موجودة على الـ .. لما يكون فيه + +330 +00:23:55,190 --> 00:23:57,510 +لسه trial بيشوفوا بعض أنه والله المناسبين مش + +331 +00:23:57,510 --> 00:24:00,830 +المناسبين هو بيروح بربطهم مع بعض فبيعمل non + +332 +00:24:00,830 --> 00:24:04,810 +specific activation .. non specific activation للـ + +333 +00:24:04,810 --> 00:24:08,130 +T cells إذا عمل non specific activation للـ T cells + +334 +00:24:08,130 --> 00:24:14,170 +هيكون عندي ردة فعل مناعية مش طبيعية exaggerated وبـ + +335 +00:24:14,170 --> 00:24:17,330 +يصير فيها مجموعة من الأعراض ممكن أتكلم عنهم بيجي + +336 +00:24:17,330 --> 00:24:20,470 +بس fever + +337 +00:24:22,820 --> 00:24:25,140 +يجب أن نركز على اللوحة لأنها برضه فيها بعض + +338 +00:24:25,140 --> 00:24:29,300 +التعبيرات هذه الكرتون مهم جدا أن الاتنين يلزقون مع + +339 +00:24:29,300 --> 00:24:32,020 +بعض يعني الـ T-cell والـ antigen-presenting cell + +340 +00:24:32,020 --> 00:24:34,460 +وهو بتاع اللي بيعمل non specific activation لكن + +341 +00:24:34,460 --> 00:24:37,920 +بينتج على هذا disease fever, rashes, desquamation + +342 +00:24:37,920 --> 00:24:41,640 +و desquamation يعني برضه موت في خلايا الجلد، + +343 +00:24:42,280 --> 00:24:46,300 +vomiting, diarrhea و myalgias myalgias اللي هي ألم + +344 +00:24:46,300 --> 00:24:50,180 +في العضلات ألم في العضلات طبعا أنا بتوقع أنه كونه + +345 +00:24:50,180 --> 00:24:54,980 +الـ toxin ومطص عبر الـ mucous membrane بجراع الدم ومدى + +346 +00:24:54,980 --> 00:25:00,520 +مجراع الدم الأعراض هتكون إيه؟ Systemic خد بالك الـ + +347 +00:25:00,520 --> 00:25:01,860 +.. الـ stuff اللي الـ*Staphylococcus aureus* + +348 +00:25:07,590 --> 00:25:10,750 +locally in the intestine عشانك الأعراض وين كانت؟ + +349 +00:25:10,750 --> 00:25:14,770 +في الـ intestinal tract، diarrhea زاد vomiting، بينما + +350 +00:25:14,770 --> 00:25:19,890 +الـ super antigen أو الـ toxic shock syndrome toxin + +351 +00:25:19,890 --> 00:25:22,750 +كان super antigen وين موجود في الدم وبالتالي + +352 +00:25:22,750 --> 00:25:26,150 +الأعراض كانت إيه؟ systemic طبعا الصورة اللي قدامنا + +353 +00:25:26,150 --> 00:25:31,930 +هنا هي box لـ tampons وcartoon لـ .. اللي هو قلنا + +354 +00:25:31,930 --> 00:25:35,570 +الـ super antigen كيف بيربط الخلايا بعض البعض و + +355 +00:25:35,570 --> 00:25:39,950 +الأخرى هي T-cell مع antigen presenting cell برضه + +356 +00:25:39,950 --> 00:25:43,110 +باين مراجعة سريعة على اللوحة catalase, coagulase, + +357 +00:25:43,330 --> 00:25:45,810 +hyaluronidase and lipase, hemolysine or + +358 +00:25:45,810 --> 00:25:49,090 +sphingomyelinase C و leukocidin exfoliative + +359 +00:25:49,090 --> 00:25:54,320 +toxin, toxic shock syndrome toxin و enterotoxin هذه + +360 +00:25:54,320 --> 00:26:00,900 +*Staphylococcus aureus* لما أنا بتكلم عن بكتيريا + +361 +00:26:00,900 --> 00:26:05,020 +موجودة على الجلد وموجودة في الأنف تتوقعوا أنها + +362 +00:26:05,020 --> 00:26:09,060 +تسبب لنا أمراض كثيرة ولا ما تسبب لنا أمراض كثيرة؟ + +363 +00:26:09,060 --> 00:26:14,980 +لو حكينا الجلد تبعنا، شعرنا يتحكى لو حكيناه، ما احنا + +364 +00:26:14,980 --> 00:26:17,540 +ممكن نخدش الجلد وما احنا مش شايفين أننا خدشنا الجلد + +365 +00:26:17,540 --> 00:26:21,080 +يدخل *Staphylococcus aureus* في بصيلة الشعر، + +366 +00:26:21,080 --> 00:26:25,590 +هتعمل لنا infection هنا كل الدمامل أو 90% من الدمامل + +367 +00:26:25,590 --> 00:26:29,430 +والخراجات اللي بتصيب الناس اللي في المجتمع mostly + +368 +00:26:29,430 --> 00:26:33,050 +*staphylococcus aureus* *staphylococcus aureus* + +369 +00:26:33,050 --> 00:26:38,750 +المكورات العنقودية الذهبية اسمها جذاب لكن واقع + +370 +00:26:38,750 --> 00:26:42,910 +الأمر هي من أخطر أنواع الميكروبات وبالتالي يجب أن + +371 +00:26:42,910 --> 00:26:46,070 +تتوقعوا في الـ clinical practice في الـ medical + +372 +00:26:46,070 --> 00:26:51,200 +laboratory أنكم تعزلوا *Staphylococcus aureus* طار + +373 +00:26:51,200 --> 00:26:55,000 +من أي ميكروب آخر من صنف الـ Gram-positive bacteria + +374 +00:26:55,000 --> 00:26:57,940 +يبقى الـ *Staphylococcus aureus* هتكون number one الـ + +375 +00:26:57,940 --> 00:27:04,060 +super star تبع الـ Gram-positive cocci من + +376 +00:27:04,060 --> 00:27:08,580 +الـ diseases الفرنكلز وهي قُبْعَة دماغ من .. يعني + +377 +00:27:08,580 --> 00:27:12,040 +قريبة من سطح الجلد دماغ من الخروجات .. قريبة من + +378 +00:27:12,040 --> 00:27:17,540 +سطح الجلد الـ Stye اللي هو التهاب بوصيلة الشعر اللي في + +379 +00:27:17,540 --> 00:27:21,200 +العين اللي بالعامية بتلقاها الشحات..نعرفها إنه شحات + +380 +00:27:21,200 --> 00:27:23,720 +يعني حبة في الـ .. في الـ .. في بوصيلة شعر العين + +381 +00:27:23,720 --> 00:27:29,020 +impetigo اسمها الحصف وحنشوف صور واقلها كمان شوية + +382 +00:27:29,020 --> 00:27:33,840 +معظم أطفالنا أصيبوا فيها في يوم من الأيام very + +383 +00:27:33,840 --> 00:27:37,920 +contagious معني جدا إذا كان طفل في المنزل مصاب + +384 +00:27:37,920 --> 00:27:40,560 +غالبا الأطفال كلهم اللي موجودين بيصابوا وغالبا + +385 +00:27:40,560 --> 00:27:45,210 +اللي كبار immune لـ .. لـ .. لـ هذه الـ bacteriaالـ + +386 +00:27:45,210 --> 00:27:48,370 +Epidermal Necrolysis والـ Exfoliative Dermatitis + +387 +00:27:48,370 --> 00:27:51,450 +بيشبهوا بعض فيه تنتين فيهم موت في خلايا الجلد + +388 +00:27:51,450 --> 00:27:55,910 +مستاكس التهاب الثدي أو التهاب الضرع عند الحيوانات + +389 +00:27:55,910 --> 00:28:01,290 +تمام الـ abscess هو عبارة عن دُمل بس deep inside زي + +390 +00:28:01,290 --> 00:28:06,590 +ما يقول brain abscess liver abscess تمام هذا بيكون + +391 +00:28:06,590 --> 00:28:13,000 +وين؟ داخلبالنسبة لها ده على الجلد، في الجلد الحبوب + +392 +00:28:13,000 --> 00:28:16,860 +اللي بتطلع على الناس اللي بحلجوا دجنوا مثلا بتطلع + +393 +00:28:16,860 --> 00:28:20,160 +الحبوب تاني يوم أو في نفس اليوم غالبا في الـ cocas + +394 +00:28:20,160 --> 00:28:24,320 +أو جسد يعني بيوصفوها بإنها it causes infection + +395 +00:28:24,320 --> 00:28:28,500 +from head to toe من أعلى الرأس لأسفل القدم نظرا + +396 +00:28:28,500 --> 00:28:32,100 +لانتشارها على الـ .. على الجلد اللي هو أكبر عضو في + +397 +00:28:32,100 --> 00:28:36,700 +جسم الإنسان اللي هو أيضا عرضة للجروح و الإصابات + +398 +00:28:36,700 --> 00:28:40,710 +بسبب احتكاكه مع البيئة الخارجية من الـ systemic + +399 +00:28:40,710 --> 00:28:45,370 +diseases بكتيريميا و غالبا بتكون من abscess أو + +400 +00:28:45,370 --> 00:28:49,490 +wound أو burn wounds اللي هي الجروح و الـ burns + +401 +00:28:49,490 --> 00:28:52,230 +اللي هي الطلوق و الخروجات أو الـ dynamite زي ما + +402 +00:28:52,230 --> 00:28:56,530 +حكينا بكتيريميا اذكركوا بس بـ .. ببعض الـ .. كل + +403 +00:28:56,530 --> 00:29:01,270 +كنتوا أصغار طلالكوا حد في الوجه و طبعا كانت نهاية + +404 +00:29:01,270 --> 00:29:04,850 +العالم انه كيف يعني حد بطل ع جاية و لا بالـ .. + +405 +00:29:04,850 --> 00:29:11,230 +فبننزل نحاول نـ .. نـ .. نزيل أعطارهالما .. لأ و + +406 +00:29:11,230 --> 00:29:17,710 +انتوا كبار افهم أنا .. أنا ناسر الان الـ .. الـ .. + +407 +00:29:17,710 --> 00:29:22,510 +الحب هذه اللي احنا حاولنا نفرغ محتواهها غالبا + +408 +00:29:22,510 --> 00:29:26,230 +بنضغط عليها بأصابعنا وبالتالي آخر مرحلة بيكون نزول + +409 +00:29:26,230 --> 00:29:30,110 +دم معاها نزول دم يعني ذلك ان احنا كسرنا الـ + +410 +00:29:30,110 --> 00:29:33,050 +capillaries في المنطقة كسرنا الـ capillaries يعني + +411 +00:29:33,050 --> 00:29:37,980 +دخلنا البكتيريا داخل الدم فقد تنتقل من مكانها إلى + +412 +00:29:37,980 --> 00:29:42,580 +أماكن أخرى بدل حب بصير لنا حبوب أو الحب نفسه تكبر + +413 +00:29:42,580 --> 00:29:45,840 +أو إنه تنتقل إلى الدم و تعملنا سكتسيميا و + +414 +00:29:45,840 --> 00:29:50,100 +بكتيريميا و تعملنا مشكلة كبيرة إذا ما حاولش إنه + +415 +00:29:50,100 --> 00:29:54,060 +نتخلص من الحبوب بيها نفسنا على الرغم من أن واحد من + +416 +00:29:54,060 --> 00:29:59,700 +طرق العلاج هو to drain .. drain يعني سحب اللي هو + +417 +00:29:59,700 --> 00:30:05,200 +السائل اللي موجود بس يسحب من طبيب بيعرف كيف يسحبوا + +418 +00:30:05,200 --> 00:30:08,860 +هذا رقم واحد، رقم اثنين بيعطينا antibiotic قبل ما + +419 +00:30:08,860 --> 00:30:13,420 +يسحبوا، عشان لو بكتيريا هربت و راحت على الدم الـ + +420 +00:30:13,420 --> 00:30:16,960 +antibiotic أيه، يقتلها، فالـ .. الـ .. الـ systemic + +421 +00:30:16,960 --> 00:30:19,680 +disease غالبا في الـ Staphylococcus aureus بتنشأ + +422 +00:30:19,680 --> 00:30:24,500 +من فوكي، من مكان محدد، زي جرح أو حبة أو الآخر، + +423 +00:30:24,500 --> 00:30:29,310 +osteomyelitis، التهاب العظم والعضلات نيومونيا + +424 +00:30:29,310 --> 00:30:32,990 +اللي هي التهاب الرئة وبنسمي الـ staphylococcus + +425 +00:30:32,990 --> 00:30:37,550 +نيومونيا باسم خاص بنسميها staphylococcus cocci pneumonia + +426 +00:30:37,550 --> 00:30:42,170 +عشان نميزها عن باقي أنواع النيومونيا هذه الـ + +427 +00:30:42,170 --> 00:30:48,430 +impetigo اللي حكينا عنها هي عبارة عن skin infection + +428 +00:30:48,430 --> 00:30:52,790 +طبعا هذه .. هذه ألطف صورة ممكن نلاقيها لو دورتوا + +429 +00:30:52,790 --> 00:30:55,330 +على الـ .. على الـ google على impetigo هتشوفوا صور + +430 +00:30:55,330 --> 00:30:57,430 +كثيرة mosaically disturbing أنا جيبت صورة يعني + +431 +00:30:57,430 --> 00:31:02,290 +نوعا ما مقبولة طبعا الـ .. الـ .. الـ .. الـ .. الـ اللي + +432 +00:31:02,290 --> 00:31:05,070 +عارفينها في الـ chin هذه بعد فترة بيصيرلها white + +433 +00:31:05,070 --> 00:31:08,550 +crustacean يعني أو .. أو golden crustacean حتى بكل + +434 +00:31:08,550 --> 00:31:11,870 +لونها ذهبي اللي هي القشرة اللي .. أو القشور وزي ما + +435 +00:31:11,870 --> 00:31:15,290 +حكيتلكوا very contagious والسلالة اللي بتسيلف هذا + +436 +00:31:15,290 --> 00:31:18,850 +النوع غالبا بتكون antimicrobial resistance يعني + +437 +00:31:18,850 --> 00:31:22,090 +انها مقاومة antimicrobial يعني ذلك بيلاقي ان + +438 +00:31:22,090 --> 00:31:26,030 +البنسالين اللي كان مناسب للدمام للخلالات غير مناسب + +439 +00:31:26,030 --> 00:31:30,190 +لعلاج اللي هو الـ MBT وهذا مؤشر بيأكد الكلام اللي + +440 +00:31:30,190 --> 00:31:34,330 +حكيناه إنه بيتكلم عن سلالات مختلفة صحيح اسم واحد + +441 +00:31:34,330 --> 00:31:38,440 +لكن سلالات مختلفة هذه الصفحة دي خلصناها تقريبا، + +442 +00:31:38,440 --> 00:31:41,280 +هتكلمنا عن الـ food poisoning وميزنا بين كلمة food + +443 +00:31:41,280 --> 00:31:44,840 +poisoning و food infection الـ poisoning إن الـ + +444 +00:31:44,840 --> 00:31:49,520 +toxin was formed in the food before eating it + +445 +00:31:49,520 --> 00:31:54,360 +بينما الـ food infection هو acted as a carrier for + +446 +00:31:54,360 --> 00:31:58,720 +the infectious agent usually food is contaminated + +447 +00:31:58,720 --> 00:32:01,640 +from humans و بصير له growth و بعدين بيصير فيه إنه + +448 +00:32:01,640 --> 00:32:02,920 +enterotoxin + +449 +00:32:05,350 --> 00:32:10,190 +بتكونوا تخيلوا موضوع .. أما موضوع food .. + +450 +00:32:10,190 --> 00:32:14,490 +poisoning و food infection هيحتل معانا course كامل + +451 +00:32:14,490 --> 00:32:15,530 +سنة الجاية + +452 +00:32:21,620 --> 00:32:24,140 +الـ Food Microbiology .. الـ Food Microbiology + +453 +00:32:24,140 --> 00:32:27,100 +صرعنا course بالخطط .. بخطط التحقيق الطبيب والتالي + +454 +00:32:27,100 --> 00:32:31,180 +بنعرف إنه إله أهمية كبرى و خلينا ناخد الـ medical + +455 +00:32:31,180 --> 00:32:35,920 +microbiology منطلق لفهم أعمق يعني بمعنى آخر التخيل + +456 +00:32:35,920 --> 00:32:40,400 +اللي أنا ذكرته في صناع غذاء محدد اللي هو المفتول + +457 +00:32:40,400 --> 00:32:45,320 +من .. من خطواته من ألف إلى الياء تمام؟ ممكن نطبق + +458 +00:32:45,320 --> 00:32:48,200 +هذه المفهومة على أي غذاء احنا بنصنعه و نشوف وين + +459 +00:32:48,200 --> 00:32:55,160 +ممكن يـ .. يصير تلوث منـ .. لـ human لأن أنظمة الجودة + +460 +00:32:55,160 --> 00:32:59,100 +بتصنيع الغذاء اليوم بتتنش على إن المنتج النهائي + +461 +00:32:59,100 --> 00:33:04,320 +يكون جيد و بس لازم من الـ raw material من المواد + +462 +00:33:04,320 --> 00:33:09,100 +الخامة إلى المنتج يكون في عملية مراقبة لخط الإنتاج + +463 +00:33:09,100 --> 00:33:11,720 +و أنا بقصد خط الإنتاج مش ضروري مصنع ممكن في البيت + +464 +00:33:11,720 --> 00:33:16,290 +كمان ما هو في الآخر خطوات ولا لأ، أي تصنيع، أي حاجة + +465 +00:33:16,290 --> 00:33:18,890 +بنعملها هي عبارة عن خطوات، تجاربنا في المختبر + +466 +00:33:18,890 --> 00:33:22,470 +عبارة عن خطوات، فمن أول خطوة إلى آخر خطوة لازم + +467 +00:33:22,470 --> 00:33:25,110 +يكون في أندي مقدرة على أنه أسيطر على العملية بحيث + +468 +00:33:25,110 --> 00:33:28,970 +أنه وين في احتمالية contamination أعمل إجراءات + +469 +00:33:28,970 --> 00:33:30,670 +تمنع اللي هو الـ contamination + +470 +00:33:35,890 --> 00:33:38,830 +الـ baby with scalded skin syndrome اللي هنقول عنهم + +471 +00:33:38,830 --> 00:33:44,090 +exfoliating اللي هو بيعمل epidermal lysis لـ الـ + +472 +00:33:44,090 --> 00:33:48,270 +skin وبالتالي إذا بتلاحظوا الصورة واضح جدا إنه في + +473 +00:33:48,270 --> 00:33:55,390 +عندنا لونين لون و لون وفي عندنا حواف لجلد بدأت + +474 +00:33:55,390 --> 00:33:59,630 +برضه كمان بيكون continuous shedding لـ الـ skin + +475 +00:33:59,630 --> 00:34:03,630 +laboratory + +476 +00:34:04,370 --> 00:34:09,030 +هناخدوا بالكوا في الـ medical microbiology هعمل من + +477 +00:34:09,030 --> 00:34:12,510 +الآن وصاعدا هعمل لوحة واحدة للـ laboratory + +478 +00:34:12,510 --> 00:34:17,810 +diagnosis عشان الـ diagnostic microbiology هو أصلا + +479 +00:34:17,810 --> 00:34:21,050 +الشغل اللي فيها الـ diagnosis وبتاع مش هنلاقي الـ + +480 +00:34:21,050 --> 00:34:25,650 +course الثاني هنا لكن معنيين إنه ناخد فكرة عامة عن + +481 +00:34:25,650 --> 00:34:28,190 +الـ laboratory diagnosis أي تفاصيل بعد اللوحة هذه + +482 +00:34:28,190 --> 00:34:33,300 +هي للشرح فقط لغاية يعني ليس مطالبين إلا باللوحة + +483 +00:34:33,300 --> 00:34:35,540 +الرئيسية اللي مكتوب عليها laboratory diagnosis لأن + +484 +00:34:35,540 --> 00:34:43,360 +تزيد عن صفحة لأن تزيد عن لوحة واحد Staphylococcus + +485 +00:34:43,360 --> 00:34:46,380 +aureus إيش سميناها؟ احنا جاب شوية من ناحية حرية الـ + +486 +00:34:46,380 --> 00:34:52,360 +oxygen في عقل تاتر عنوغرخص نعمل incubation هوائي + +487 +00:34:52,360 --> 00:34:57,000 +ولا لا هوائي؟ هوائي اللي لا هوائي مع الحاجة دي بيزيل + +488 +00:34:57,000 --> 00:35:01,720 +الهواء، إذا توفير بيئة لا هوائية هو بيكلفنا فلوس + +489 +00:35:01,720 --> 00:35:04,340 +لإن احنا بدنا نضطر إنه ننزع الهواء اللي هو أصلا + +490 +00:35:04,340 --> 00:35:10,460 +موجود بشكل natural ممكن .. قولنالكوا العينة اللي + +491 +00:35:10,460 --> 00:35:13,340 +فيها صديد لو عملناها gram stain مباشرة نشوف + +492 +00:35:13,340 --> 00:35:17,720 +البكتيريا، لكن هل يمكن من خلالها إنه نفتي أن + +493 +00:35:17,720 --> 00:35:20,980 +البكتيريا استفيد من cocas aureas؟ لأ، بتساعدنا + +494 +00:35:21,910 --> 00:35:26,470 +زراعتنا على مين نزرع؟ على الـ blood agar كثير بتنمو + +495 +00:35:26,470 --> 00:35:29,850 +بشكل جيد على الـ blood agar وإنها مميزات على الـ + +496 +00:35:29,850 --> 00:35:33,830 +blood agar في المحاضرة الماضية وأنا قاعد بشرح على + +497 +00:35:33,830 --> 00:35:38,370 +أهمية شكل المستعمرة حاولت أوصف المستعمرة تبعت الـ + +498 +00:35:38,370 --> 00:35:42,190 +Staphylococcus aureus على الـ blood agar فقول large + +499 +00:35:42,190 --> 00:35:45,850 +two to four millimeter rounded golden yellow + +500 +00:35:45,850 --> 00:35:52,240 +pigmented colonies on blood agar واليوم المضيف انها + +501 +00:35:52,240 --> 00:35:55,720 +hemolytic فاكرين الـ hemolycine؟ يعني بتاع ما الـ + +502 +00:35:55,720 --> 00:35:59,520 +beta hemolysis الآن خليني أتكلم عن الـ beta + +503 +00:35:59,520 --> 00:36:02,720 +hemolysis والـ alpha hemolysis والـ no hemolysis + +504 +00:36:02,720 --> 00:36:06,640 +راجعوا معايا في الكلمات اللي بتحكيها من مولودة + +505 +00:36:06,640 --> 00:36:13,100 +على هذا السبورة الـ blood agar الـ blood agar إذا + +506 +00:36:13,100 --> 00:36:17,920 +حطينا عليه بكتيريا فهناك ثلاث احتمالات تتعلق بالـ + +507 +00:36:17,920 --> 00:36:23,900 +hemolysis الاحتمال الأول أن يكون هناك تحلل كامل + +508 +00:36:23,900 --> 00:36:30,920 +للدم حول المستعمرة ويطلق على هذا النوع من التحلل + +509 +00:36:30,920 --> 00:36:35,660 +Beta Hemolysis Complete Hemolysis Beta Hemolysis + +510 +00:36:35,660 --> 00:36:39,360 +كيف بنعرف إنه Beta Hemolysis لون الـ blood agar + +511 +00:36:39,360 --> 00:36:42,960 +بيكون أحمر بيصير colorless يعني المستعمرة بيصير + +512 +00:36:42,960 --> 00:36:48,920 +حولها منطقة خالية من اللون خالي من اللون للمستعمرة + +513 +00:36:48,920 --> 00:36:54,740 +هذا بيتا هموليسيس الاحتمال الثاني incomplete + +514 +00:36:54,740 --> 00:37:00,860 +hemolysis أو partial طبعا مصطلحها كويس partial أو + +515 +00:37:00,860 --> 00:37:06,800 +incomplete hemolysis كيف بنصدر عليه بيصير لون الدم + +516 +00:37:06,800 --> 00:37:11,460 +بدل ما هو أحمر أخضر نعم greenish لأنه incomplete + +517 +00:37:11,460 --> 00:37:12,180 +hemolysis + +518 +00:37:15,870 --> 00:37:20,990 +وبنسميها Alpha الاحتمال + +519 +00:37:20,990 --> 00:37:26,410 +الثالث أن لا يتغير شيء يعني لا هموليسيس لكمبيوتر + +520 +00:37:26,410 --> 00:37:29,710 +لإن كمبيوتر بنسميها No Hemolysis ويلها اسم عشان ما + +521 +00:37:29,710 --> 00:37:33,970 +تزعلش Gamma Hemolysis يبقى عندنا بنقول Gamma + +522 +00:37:33,970 --> 00:37:38,630 +Hemolysis جاهزة ترضية جاهزة ترضية للبكتيريا اللي + +523 +00:37:38,630 --> 00:37:43,310 +بتعملش Hemolysis إذا Beta Alpha Gamma + +524 +00:37:49,370 --> 00:37:52,510 +ماعندناش هذه المعلومات الهامش، هستفيد منها لاحقا إن + +525 +00:37:52,510 --> 00:37:56,070 +شاء الله لما أتكلم عن الـ laboratory diagnosis الـ + +526 +00:37:56,070 --> 00:37:59,810 +differential test منطول كواجوليز و DNA هاي + +527 +00:37:59,810 --> 00:38:02,350 +الفيصولات اللي هتستخدمها في التفريق بينها وبين + +528 +00:38:02,350 --> 00:38:07,350 +السللات الأخرى أو اللي اتقفن الـ spaces الأخرى من + +529 +00:38:07,350 --> 00:38:11,330 +الـ staphylococcus بينما إذا بدي أميزها عن + +530 +00:38:11,330 --> 00:38:15,250 +الـ streptococci إيش بدي كتاليز + +531 +00:38:26,690 --> 00:38:31,470 +الفحص في مكانه المناسب لا يلزم أن أول فحص في غير + +532 +00:38:31,470 --> 00:38:34,730 +مكان المناسب يعني أنت تعلمت في المختبر عشرين فحص + +533 +00:38:34,730 --> 00:38:40,950 +أو ثلاثين فحص مش تجيبهم كلهم اختيارهم مبني على + +534 +00:38:40,950 --> 00:38:45,810 +مبني على معلومات تتبع معلومات، أعطيكم مثال، عزرت + +535 +00:38:45,810 --> 00:38:49,270 +بكتيريا أنا على طبق اسمه بلداجات، عملتلها gram + +536 +00:38:49,270 --> 00:38:54,410 +stain، طلعت gram-positive cocci، what is next؟ + +537 +00:38:54,410 --> 00:38:59,970 +كتاليز، طلعت negative، كتاليز negative، يعني إيش + +538 +00:38:59,970 --> 00:39:00,050 +الـ + +539 +00:39:13,400 --> 00:39:18,640 +اختياري للفحوصات كان معتمد على مين؟ على تسلسل .. + +540 +00:39:18,640 --> 00:39:24,240 +تسلسل .. أحداث معينة ما بجيش أنا أقول الـ + +541 +00:39:24,240 --> 00:39:28,700 +Staphylococcus aureus جلوكوز فيرمنتر والـ E.coli + +542 +00:39:28,700 --> 00:39:30,580 +جلوكوز فيرمنتر كيف ده نميزوا من بعض؟ + +543 +00:39:34,680 --> 00:39:37,520 +طب واحدة كوكساية واحدة بسالاية يعني هم أساسا + +544 +00:39:37,520 --> 00:39:40,900 +مختلفين ليش أنا .. أنا .. ليش أخطأت وجبت صفة + +545 +00:39:40,900 --> 00:39:43,980 +تتشارك مع بعض فيهم عشان أميزهم مع بعض أساسا هم + +546 +00:39:43,980 --> 00:39:48,520 +المميزين الأبيض والأسود تمام؟ هم + +547 +00:39:48,520 --> 00:39:51,700 +أصلا مميزين وبالتالي ما فيش داعي أن أعتمد على صفات + +548 +00:39:51,700 --> 00:39:56,600 +أخرى أعقدهم الموضوع قد تتشارك الميكروبات في صفات + +549 +00:39:56,600 --> 00:39:59,900 +معينة لكن من الأساس أنا أميزهم بالـ Gram Stain Gram + +550 +00:39:59,900 --> 00:40:03,470 +Positive والـ Gram Negative بسالاية أو كوكساية إذن + +551 +00:40:03,470 --> 00:40:07,090 +عشان أنا أقدر أقول إن البكتيريا هي استفيد لكوكاين + +552 +00:40:07,090 --> 00:40:10,290 +لازم يكون عندي معلومتين إنها gram-positive cocci + +553 +00:40:10,290 --> 00:40:17,310 +إذا اعتبرتوها معلومتين لحالهم فالتلت معلومات gram + +554 +00:40:17,310 --> 00:40:19,150 +-positive cocci + +555 +00:40:21,680 --> 00:40:26,160 +التلت معلومات لحد الآن لحد اللحظة هذه بيشيروا إن + +556 +00:40:26,160 --> 00:40:29,420 +البكتيريا هي Staphylococcus مين من الـ + +557 +00:40:29,420 --> 00:40:33,880 +Staphylococci؟ المانيتول والـ coagulase والـ DNA's + +558 +00:40:33,880 --> 00:40:36,240 +اللي مردها الصبورة ده اللي هم اللي بيميزوه منهم + +559 +00:40:36,240 --> 00:40:44,660 +الـ phage typing فاكرين الفاش؟ البكتيريا فاج البكتيريا فاج + +560 +00:40:44,660 --> 00:40:49,140 +كان فيه منه نوع سميناه lytic phage اللي بيخش على + +561 +00:40:49,140 --> 00:40:53,880 +البكتيريا وبيحللها اللوحة اللي قدامكوا هذه الصورة + +562 +00:40:53,880 --> 00:40:58,560 +طبق هو عبارة عن طبقة من البكتيريا نامية وفي مناطق + +563 +00:40:58,560 --> 00:41:04,860 +فارغة من النمو black plaques اللي هي اللي هي الأثر + +564 +00:41:04,860 --> 00:41:08,460 +اللي بتركه البكتيريا فاج من تحلل الخلايا منها + +565 +00:41:08,460 --> 00:41:12,520 +plaques وإذا بتذكروا كنا نعدل الفيروسات عن طريق الـ + +566 +00:41:12,520 --> 00:41:17,330 +plaque بنقول plaque warming unit الآن لو أنا عندي + +567 +00:41:17,330 --> 00:41:21,290 +بكتيريا مجهولة اسمها staphylococcus تمام؟ وبدي + +568 +00:41:21,290 --> 00:41:25,650 +أعرفها aureus ولا epidermis وأنا عندي في التلاجة + +569 +00:41:25,650 --> 00:41:28,730 +bacteriophage للـ aureus وواحد للـ epidermis وواحد + +570 +00:41:28,730 --> 00:41:32,830 +للـ saprophytic والبكتيريا اللي قدامي هذه بدي أعرف + +571 +00:41:32,830 --> 00:41:36,610 +إنها aureus ولا لا حاطط عليها bacteriophage زي ما + +572 +00:41:36,610 --> 00:41:41,090 +تشوفين في الصورة تمام؟ حاطط عليها bacteriophage + +573 +00:41:41,400 --> 00:41:44,200 +تاني يوم البكتيريا نمت لك اللي جت فيها ثقوب معناه + +574 +00:41:44,200 --> 00:41:48,080 +اللي حكى أشهر إن البكتيريا فاج specific لها معناه + +575 +00:41:48,080 --> 00:41:52,460 +اللي حكى إن البكتيريا فاج specific هي مجهولة هو + +576 +00:41:52,460 --> 00:41:55,920 +معلوم الآن التفاعل كمان بتتكلم عن التفاعل + +577 +00:41:55,920 --> 00:41:59,700 +specific التفاعل المجهول مع المعلوم أصبح المجهول + +578 +00:41:59,700 --> 00:42:04,080 +علمه وتاني من جثصنا يبقى عملنا بالفعل معرفة أو + +579 +00:42:04,080 --> 00:42:08,680 +تعرف على البكتيريا من خلال اللي هو البكتيريا فاج + +580 +00:42:08,680 --> 00:42:14,920 +هنا الـ antibiotic sensitivity testing لما أنا بشرح + +581 +00:42:14,920 --> 00:42:20,560 +محاضرات عامة عن الـ antimicrobial resistance أول + +582 +00:42:20,560 --> 00:42:26,700 +قصة ببدأ فيها كنموذج عن الـ resistance بتكلم عن الـ + +583 +00:42:26,700 --> 00:42:31,060 +Staphylococcus aureus في أربعينيات القرن الماضي + +584 +00:42:31,060 --> 00:42:35,580 +كانت الـ Staphylococcus aureus وزي ما ذكرتلكوا الـ + +585 +00:42:35,580 --> 00:42:38,020 +wound infection والـ birth infection والدنامل والـ + +586 +00:42:38,020 --> 00:42:42,680 +الخرجات يعني منتشرة في العالم بشكل كبير جدا إيجي + +587 +00:42:42,680 --> 00:42:48,660 +البنسلين وكان الـ magic drug فاش + +588 +00:42:48,660 --> 00:42:50,880 +يستفيد الكوكا الـ infection كانت تصمد قدام + +589 +00:42:50,880 --> 00:42:57,020 +البنسلين بعد 15 سنة بدأت تظهر نتائج سيئة للغاية + +590 +00:42:57,020 --> 00:43:00,320 +للبنسالين البنسالين بطل صناعة الـ injection والـ + +591 +00:43:00,320 --> 00:43:04,600 +double injection على الفاضي resistance اكتشفنا + +592 +00:43:04,600 --> 00:43:08,060 +عملنا modification للبنسالين عملنا وكسلين وأمبيسلين + +593 +00:43:08,060 --> 00:43:12,500 +هدولا semisynthetic حاليا فيهم بعض الإشكاليات بعد + +594 +00:43:12,500 --> 00:43:16,120 +سنتين تلاتة بس بدأ يكون Resistance كوّننا Oxycelin + +595 +00:43:16,120 --> 00:43:18,400 +وميثيسيلين كوّنت Resistance صار اسمها + +596 +00:43:18,400 --> 00:43:21,140 +Methicillin Resistance Staphylococcus Aureus أو + +597 +00:43:21,140 --> 00:43:24,920 +MRSA مشهور جدا هذه البكتيريا إجرينا Vancomycin + +598 +00:43:24,920 --> 00:43:30,440 +وقلنا هذا الحل Vancomycin هو الحل فبعد عدد سنوات + +599 +00:43:30,440 --> 00:43:34,120 +لجينا VISA VISA Vancomycin Intermediate + +600 +00:43:34,120 --> 00:43:36,320 +Staphylococcus Aureus بعد كام من سنة لجينا + +601 +00:43:36,320 --> 00:43:40,780 +Vancomycin Resistance Staphylococcus Aureus يبقى + +602 +00:43:40,780 --> 00:43:44,880 +البكتيريا دي شمالها يعني إيش أنتو بتعملوا + +603 +00:43:44,880 --> 00:43:48,320 +antibiotics تمام؟ هي عند المقضرة عينها تكون + +604 +00:43:48,320 --> 00:43:51,920 +resistance وبالتالي أنا صرت أخاف كتير من هذه + +605 +00:43:51,920 --> 00:43:55,060 +البكتيريا باعتبرها من الميكروبات الخطيرة جدا وكتير + +606 +00:43:55,060 --> 00:43:58,540 +من الدراسات اللي أجريناها الصحيح أجريناها على الـ + +607 +00:43:58,540 --> 00:44:01,920 +Staphylococcus aureus لجينا على سبيل المثال عند + +608 +00:44:01,920 --> 00:44:05,160 +الأخوة والأخوات اللي عاملين في مستشفى الشفاء لما + +609 +00:44:05,160 --> 00:44:09,260 +خدنا الموبايلات بتاعتهم لجينا نسبة منهم عندهم MRSA + +610 +00:44:09,260 --> 00:44:12,060 +على الموبايلات يعني بيروحوها على الدار معاهم + +611 +00:44:12,060 --> 00:44:16,240 +وبيعطوها للولاد وولادهم بيعطوها للناس تاني فبتصيب + +612 +00:44:16,240 --> 00:44:20,100 +المستشفى كائن هي مجتمع فاكرين لما قسمنا احنا كنا + +613 +00:44:20,100 --> 00:44:23,580 +hospital infection وcommunity acquired infection + +614 +00:44:23,580 --> 00:44:28,280 +اللي احنا بتكلم على إنه مجتمع شبيه بمين ببيت طبعا + +615 +00:44:28,280 --> 00:44:31,660 +ماوصلنا احنا للمستشفى لكن على الأقل المستشفى واضح + +616 +00:44:31,660 --> 00:44:35,100 +أنه بيعمل دخول لمايكروبات تبعته في الـ community + +617 +00:44:35,100 --> 00:44:39,060 +تبعته الـ antibiotic sensitivity أنا اختبرت فيها + +618 +00:44:39,060 --> 00:44:43,750 +المحاضرة اللي فاتت لما أتكلمت عن الـ diagnosis أو الـ + +619 +00:44:43,750 --> 00:44:46,230 +detection وprevention أو prophylaxis and + +620 +00:44:46,230 --> 00:44:49,630 +treatment قلتلكوا أنا احنا مسؤوليتنا في المختبر + +621 +00:44:49,630 --> 00:44:53,210 +نعزل الميكروب اللي هو الـ pathogen من العينة و + +622 +00:44:53,210 --> 00:44:57,550 +نتعرف عليه ونعمل له antimicrobial sensitivity أو + +623 +00:44:57,550 --> 00:45:01,010 +susceptibility نفس المعنى testing اختيارنا للـ + +624 +00:45:01,010 --> 00:45:05,730 +antibiotic اللي هنستخدمهم منطبق اسم البكتيريا منطبق + +625 +00:45:05,730 --> 00:45:10,170 +الأمر تبع المريض يعني أعطيكم مثال على الأمر تعرف + +626 +00:45:10,170 --> 00:45:14,710 +تتراسيكلين؟ تتراسيكلين، antibiotic مشهور جدا و + +627 +00:45:14,710 --> 00:45:18,910 +very effective أو على الأقل كان very effective لكن + +628 +00:45:18,910 --> 00:45:25,450 +لا يجوز إعطاؤه لا للطفل ولا للمرأة الحامل ولا + +629 +00:45:25,450 --> 00:45:30,170 +للمرأة المرضى، فالمرضى إحنا خايفين على مش الأم + +630 +00:45:30,170 --> 00:45:36,390 +خايفين على الجنين وفي الحامل خايفين على الجنين وال + +631 +00:45:36,390 --> 00:45:40,830 +طفل خايفين على الطفل طبعا يعني ما بنقدرش نعطيه، + +632 +00:45:40,830 --> 00:45:42,790 +فأنا أقول يا عزازة أنا استفيد الكوكس سيل من + +633 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0.9736328125}, {"start": 2134.47, "end": 2135.21, "word": " المستعمرة", "probability": 0.97265625}, {"start": 2135.21, "end": 2136.23, "word": " حاولت", "probability": 0.975830078125}, {"start": 2136.23, "end": 2136.87, "word": " أوصف", "probability": 0.9446614583333334}, {"start": 2136.87, "end": 2137.99, "word": " المستعمرة", "probability": 0.9793701171875}, {"start": 2137.99, "end": 2138.27, "word": " تبعت", "probability": 0.707763671875}, {"start": 2138.27, "end": 2138.37, "word": " ال", "probability": 0.50537109375}, {"start": 2138.37, "end": 2139.09, "word": " Staphylococcus", "probability": 0.823486328125}, {"start": 2139.09, "end": 2139.57, "word": " aureus", "probability": 0.8173828125}, {"start": 2139.57, "end": 2139.81, "word": " على", "probability": 0.90380859375}, {"start": 2139.81, "end": 2140.11, "word": " ال", "probability": 0.912109375}, {"start": 2140.11, "end": 2140.27, "word": " blood", "probability": 0.95361328125}, {"start": 2140.27, "end": 2140.57, "word": " agar", "probability": 0.986083984375}, {"start": 2140.57, "end": 2140.91, "word": " فقول", "probability": 0.80908203125}, {"start": 2140.91, "end": 2142.19, "word": " large", "probability": 0.33837890625}, {"start": 2142.19, "end": 2142.43, "word": " two", "probability": 0.447509765625}, {"start": 2142.43, "end": 2142.59, "word": " to", "probability": 0.9423828125}, {"start": 2142.59, "end": 2142.87, "word": " four", "probability": 0.94921875}, {"start": 2142.87, "end": 2143.47, "word": " millimeter", "probability": 0.387939453125}, {"start": 2143.47, "end": 2144.61, "word": " rounded", "probability": 0.6591796875}, {"start": 2144.61, "end": 2145.61, "word": " golden", "probability": 0.8037109375}, {"start": 2145.61, "end": 2145.85, "word": " yellow", "probability": 0.66552734375}, {"start": 2145.85, "end": 2146.53, "word": " pigmented", "probability": 0.947265625}, {"start": 2146.53, "end": 2148.53, "word": " colonies", "probability": 0.8623046875}, {"start": 2148.53, "end": 2148.75, "word": " on", "probability": 0.86328125}, {"start": 2148.75, "end": 2148.93, "word": " blood", "probability": 0.92626953125}, {"start": 2148.93, "end": 2149.25, "word": " agar", "probability": 0.975830078125}], "temperature": 1.0}, {"id": 85, "seek": 217848, "start": 2150.46, "end": 2178.48, "text": "واليوم المضيف انها hemolytic فاكريني ال hemolycine؟ يعني بتاع ما ال beta hemolysis الان خليني اتكلم عن ال beta hemolysis و ال alpha hemolysis و ال no hemolysis راجزوا معايا في الكلامات اللي بتحكيها من مولودة على هذا السبورة ال blood agar ال blood agar إذا زولت عليه بكتيريا فهناك ثلاث احتمالات تتعلق بال hemolysis", "tokens": [2407, 6027, 1829, 20498, 9673, 11242, 33911, 16472, 11296, 415, 3280, 356, 40907, 6156, 995, 37983, 9957, 1829, 2423, 415, 3280, 356, 66, 533, 22807, 37495, 22653, 39894, 45761, 19446, 2423, 9861, 415, 3280, 356, 17122, 2423, 7649, 16490, 1211, 9957, 1829, 1975, 2655, 4117, 19528, 18871, 2423, 9861, 415, 3280, 356, 17122, 4032, 2423, 8961, 415, 3280, 356, 17122, 4032, 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"probability": 0.77001953125}, {"start": 2153.54, "end": 2153.62, "word": " ال", "probability": 0.96044921875}, {"start": 2153.62, "end": 2154.56, "word": " hemolycine؟", "probability": 0.610107421875}, {"start": 2154.56, "end": 2155.28, "word": " يعني", "probability": 0.7269287109375}, {"start": 2155.28, "end": 2155.46, "word": " بتاع", "probability": 0.6934814453125}, {"start": 2155.46, "end": 2155.56, "word": " ما", "probability": 0.27685546875}, {"start": 2155.56, "end": 2155.72, "word": " ال", "probability": 0.91552734375}, {"start": 2155.72, "end": 2156.02, "word": " beta", "probability": 0.83642578125}, {"start": 2156.02, "end": 2156.76, "word": " hemolysis", "probability": 0.84521484375}, {"start": 2156.76, "end": 2157.18, "word": " الان", "probability": 0.50579833984375}, {"start": 2157.18, "end": 2157.74, "word": " خليني", "probability": 0.78594970703125}, {"start": 2157.74, "end": 2157.96, "word": " اتكلم", "probability": 0.8961181640625}, {"start": 2157.96, "end": 2158.22, "word": " عن", "probability": 0.78466796875}, {"start": 2158.22, "end": 2158.64, "word": " ال", "probability": 0.9609375}, {"start": 2158.64, "end": 2159.52, "word": " beta", "probability": 0.84716796875}, {"start": 2159.52, "end": 2160.06, "word": " hemolysis", "probability": 0.9267578125}, {"start": 2160.06, "end": 2160.38, "word": " و", "probability": 0.7607421875}, {"start": 2160.38, "end": 2160.62, "word": " ال", "probability": 0.7666015625}, {"start": 2160.62, "end": 2160.82, "word": " alpha", "probability": 0.85986328125}, {"start": 2160.82, "end": 2161.3, "word": " hemolysis", "probability": 0.9456787109375}, {"start": 2161.3, "end": 2161.46, "word": " و", "probability": 0.9033203125}, {"start": 2161.46, "end": 2161.56, "word": " ال", "probability": 0.9208984375}, {"start": 2161.56, "end": 2161.78, "word": " no", "probability": 0.78466796875}, {"start": 2161.78, "end": 2162.72, "word": " hemolysis", "probability": 0.95361328125}, {"start": 2162.72, "end": 2164.96, "word": " راجزوا", "probability": 0.79412841796875}, {"start": 2164.96, "end": 2165.22, "word": " معايا", "probability": 0.90478515625}, {"start": 2165.22, "end": 2165.48, "word": " في", "probability": 0.7724609375}, {"start": 2165.48, "end": 2166.0, "word": " الكلامات", "probability": 0.809326171875}, {"start": 2166.0, "end": 2166.1, "word": " اللي", "probability": 0.91357421875}, {"start": 2166.1, "end": 2166.32, "word": " بتحكيها", "probability": 0.824169921875}, {"start": 2166.32, "end": 2166.4, "word": " من", "probability": 0.53369140625}, {"start": 2166.4, "end": 2166.64, "word": " مولودة", "probability": 0.59417724609375}, {"start": 2166.64, "end": 2166.72, "word": " على", "probability": 0.7529296875}, {"start": 2166.72, "end": 2166.86, "word": " هذا", "probability": 0.0963134765625}, {"start": 2166.86, "end": 2168.12, "word": " السبورة", "probability": 0.783935546875}, {"start": 2168.12, "end": 2169.76, "word": " ال", "probability": 0.81884765625}, {"start": 2169.76, "end": 2169.98, "word": " blood", "probability": 0.8916015625}, {"start": 2169.98, "end": 2170.46, "word": " agar", "probability": 0.829833984375}, {"start": 2170.46, "end": 2170.88, "word": " ال", "probability": 0.431396484375}, {"start": 2170.88, "end": 2171.22, "word": " blood", "probability": 0.97509765625}, {"start": 2171.22, "end": 2171.76, "word": " agar", "probability": 0.980224609375}, {"start": 2171.76, "end": 2173.1, "word": " إذا", "probability": 0.776123046875}, {"start": 2173.1, "end": 2173.36, "word": " زولت", "probability": 0.7875162760416666}, {"start": 2173.36, "end": 2173.54, "word": " عليه", "probability": 0.9345703125}, {"start": 2173.54, "end": 2174.18, "word": " بكتيريا", "probability": 0.9736328125}, {"start": 2174.18, "end": 2175.44, "word": " فهناك", "probability": 0.9569091796875}, {"start": 2175.44, "end": 2175.68, "word": " ثلاث", "probability": 0.83154296875}, {"start": 2175.68, "end": 2176.54, "word": " احتمالات", "probability": 0.95537109375}, {"start": 2176.54, "end": 2177.78, "word": " تتعلق", "probability": 0.9642333984375}, {"start": 2177.78, "end": 2177.92, "word": " بال", "probability": 0.98583984375}, {"start": 2177.92, "end": 2178.48, "word": " hemolysis", "probability": 0.8743896484375}], "temperature": 1.0}, {"id": 86, "seek": 220536, "start": 2179.84, "end": 2205.36, "text": "الاحتمال الاول ان يكون هناك تحلل كامل للدم حول المستعمرة ويطلق على هذا النوع من التحلل Beta Hemolysis Complete Hemolysis Beta Hemolysis كيف بنعرف انه Beta Hemolysis لون ال blood agar بيكون أحمر بيصير colorless يعني المستعمرة بيصير حولها منطقة خالية من اللون", "tokens": [6027, 995, 33753, 2304, 6027, 42963, 12610, 16472, 7251, 30544, 34105, 4117, 6055, 5016, 1211, 1211, 9122, 10943, 1211, 24976, 40448, 11331, 12610, 9673, 14851, 25957, 25720, 4032, 1829, 9566, 1211, 4587, 15844, 23758, 28239, 45367, 9154, 16712, 5016, 1211, 1211, 33286, 18568, 401, 4642, 34687, 18568, 401, 4642, 33286, 18568, 401, 4642, 9122, 33911, 44945, 3615, 28480, 16472, 3224, 33286, 18568, 401, 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للدم", "probability": 0.91162109375}, {"start": 2185.08, "end": 2185.5, "word": " حول", "probability": 0.9443359375}, {"start": 2185.5, "end": 2186.16, "word": " المستعمرة", "probability": 0.981201171875}, {"start": 2186.16, "end": 2188.7, "word": " ويطلق", "probability": 0.85869140625}, {"start": 2188.7, "end": 2188.98, "word": " على", "probability": 0.8984375}, {"start": 2188.98, "end": 2189.3, "word": " هذا", "probability": 0.83740234375}, {"start": 2189.3, "end": 2189.58, "word": " النوع", "probability": 0.963134765625}, {"start": 2189.58, "end": 2189.8, "word": " من", "probability": 0.98046875}, {"start": 2189.8, "end": 2190.92, "word": " التحلل", "probability": 0.99072265625}, {"start": 2190.92, "end": 2191.66, "word": " Beta", "probability": 0.447021484375}, {"start": 2191.66, "end": 2192.68, "word": " Hemolysis", "probability": 0.6931966145833334}, {"start": 2192.68, "end": 2193.64, "word": " Complete", "probability": 0.495361328125}, {"start": 2193.64, "end": 2194.36, "word": " Hemolysis", "probability": 0.9669596354166666}, {"start": 2194.36, "end": 2195.08, "word": " Beta", "probability": 0.297607421875}, {"start": 2195.08, "end": 2195.66, "word": " Hemolysis", "probability": 0.9723307291666666}, {"start": 2195.66, "end": 2196.5, "word": " كيف", "probability": 0.93212890625}, {"start": 2196.5, "end": 2196.98, "word": " بنعرف", "probability": 0.8767903645833334}, {"start": 2196.98, "end": 2197.22, "word": " انه", "probability": 0.78515625}, {"start": 2197.22, "end": 2197.4, "word": " Beta", "probability": 0.76513671875}, {"start": 2197.4, "end": 2197.96, "word": " Hemolysis", "probability": 0.9788411458333334}, {"start": 2197.96, "end": 2198.86, "word": " لون", "probability": 0.697509765625}, {"start": 2198.86, "end": 2198.96, "word": " ال", "probability": 0.599609375}, {"start": 2198.96, "end": 2199.1, "word": " blood", "probability": 0.7431640625}, {"start": 2199.1, "end": 2199.36, "word": " agar", "probability": 0.52911376953125}, {"start": 2199.36, 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هو ال pathogen من العينة و نتعرف عليه و نعمله antimicrobial sensitivity او susceptibility نفس المعنى testing اختيارنا لل antibiotic اللي هنستخدمهم منطب اسم البكتيريا منطب الأمر تبع المريض يعني اعطيكم مثال على الأمر", "tokens": [1211, 15042, 1975, 2655, 4117, 19528, 2655, 18871, 2423, 15217, 1975, 2407, 2423, 17784, 4032, 14630, 1975, 2407, 17051, 5088, 578, 271, 293, 5032, 12174, 1211, 2655, 23275, 14407, 1975, 8315, 1975, 5016, 8315, 47524, 19986, 12610, 1829, 36081, 8315, 8978, 9673, 46456, 26890, 8717, 3615, 11622, 1211, 9673, 1829, 4117, 32887, 3555, 13672, 1829, 31439, 2423, 3100, 8799, 9154, 18863, 9957, 3660, 4032, 8717, 2655, 3615, 28480, 47356, 4032, 8717, 25957, 43761, 46141, 299, 16614, 831, 19392, 1975, 2407, 26104, 2841, 8717, 36178, 9673, 3615, 1863, 7578, 4997, 1975, 9778, 31371, 9640, 8315, 24976, 37828, 13672, 1829, 8032, 1863, 14851, 9778, 40448, 16095, 9154, 9566, 3555, 24525, 2304, 29739, 4117, 2655, 13546, 25528, 9154, 9566, 3555, 16247, 29973, 6055, 3555, 3615, 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a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/-3skSIY6DA0_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/-3skSIY6DA0_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..668643d9e1cc8b5bacf06f0d1e8459cd804ebda6 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/-3skSIY6DA0_raw.srt @@ -0,0 +1,2588 @@ +1 +00:00:21,110 --> 00:00:24,130 +بسم الله الرحمن الرحيم chapter جديد و احنا ممكن + +2 +00:00:24,130 --> 00:00:27,410 +اتحدثنا سابق سابقا انه عنوان ال course تبعنا + +3 +00:00:27,410 --> 00:00:30,330 +essential medical microbiology كل مدرستناها في + +4 +00:00:30,330 --> 00:00:33,110 +السابق هو essential و اليوم بنتكلم عن ال medical + +5 +00:00:33,110 --> 00:00:37,130 +microbiology هنبدأ في عنوان ضخم اسمه biogenic + +6 +00:00:37,130 --> 00:00:44,410 +cocci وهي المكورات المقيحة بمعنى انه بيصدر عنها او + +7 +00:00:44,410 --> 00:00:47,590 +عن ال infection تبعتها قيح أو اللي احنا بسميه صديق + +8 +00:00:47,590 --> 00:00:56,270 +فهي biogenic معناهاطيحية أو صديدية الصورة + +9 +00:00:56,270 --> 00:01:00,390 +اللي أمامكم عبارة عن تشكيلة من الكوكساي اللي + +10 +00:01:00,390 --> 00:01:06,510 +موجودة اللون الأزرق أو ال violet اللي واضح هنا هي + +11 +00:01:06,510 --> 00:01:10,850 +Staphylococcus واضحة إنها grape-like clusters ال + +12 +00:01:10,850 --> 00:01:14,490 +arrangement تبعتها اللي أسفل منها هي عبارة عن + +13 +00:01:14,490 --> 00:01:18,730 +Staphylococci لاحظوا إنه ال chains very long طويل + +14 +00:01:18,730 --> 00:01:23,720 +جدا السلاسلاللي على اليسار في اندهانة Diplococci + +15 +00:01:23,720 --> 00:01:28,800 +لكن الشريحة مش pure زي الأولانية و التانية و في + +16 +00:01:28,800 --> 00:01:31,760 +إيها خلفية و الخلفية هذه حقيقة اللون الأحمر اللي + +17 +00:01:31,760 --> 00:01:36,160 +شايفينه هذا و اللي جواته أنوية هي عبارة عن pus + +18 +00:01:36,160 --> 00:01:40,420 +cells خلايا صدرية، إيش هي ال pus cells؟ هي عبارة + +19 +00:01:40,420 --> 00:01:44,440 +عن phagocytes، طب إيش ال phagocytes؟ هي neutral + +20 +00:01:44,440 --> 00:01:50,520 +fieldsالنيتروفيلز الاسم تاني polymorphonuclear + +21 +00:01:50,520 --> 00:01:55,340 +cells كلمة polymorphonuclear nuclear يعني أنوية و + +22 +00:01:55,340 --> 00:02:00,760 +لا لأ polymorpho يعني متعددة الأشكال الأنوية طلعوا + +23 +00:02:00,760 --> 00:02:08,560 +الأنوية تبعتها تمام بالمناسبة + +24 +00:02:08,560 --> 00:02:14,340 +إيش لونها كل خلايا ال human لو صبغناها gram + +25 +00:02:14,340 --> 00:02:19,160 +نيجاتيكيعني خلايانا احنا gram negative يعني بقى + +26 +00:02:19,160 --> 00:02:25,200 +بتاخد اللون الأحمر تاخد اللون الأحمر اذا اللي + +27 +00:02:25,200 --> 00:02:30,900 +بنطلع من اللوحة دي معلومتان صغار ان البكتيريا ممكن + +28 +00:02:30,900 --> 00:02:35,320 +نشوفها في عينات مباشرة وانا المحاضرة الماضية + +29 +00:02:35,320 --> 00:02:38,520 +اتشرحت قلت ان ممكن ناخد عينات CSF ونعملها + +30 +00:02:38,520 --> 00:02:42,660 +centrifigation ونعمل staining إليها مباشرةوبالتالي + +31 +00:02:42,660 --> 00:02:46,960 +هشوف بكتيريا إذا موجودة و هشوف أيضا bus cells إذا + +32 +00:02:46,960 --> 00:02:52,740 +موجودة هذا الترتيب تبع الخلايا هنا هو عبارة عن + +33 +00:02:52,740 --> 00:02:58,220 +Diplococci Diplococci يعني two coccyge بالمناسبة + +34 +00:02:58,220 --> 00:03:02,880 +ال Diplococci تصنف عليها streptococci يعني كل + +35 +00:03:02,880 --> 00:03:07,000 +اللوحة streptococci معالمين مع ال clusters اللي + +36 +00:03:07,000 --> 00:03:10,340 +علي اليمين اللي هي staphylococci + +37 +00:03:13,840 --> 00:03:18,620 +هذه كمان صورة بنرجع فيها شوية، لحظة الصورة بتوضح + +38 +00:03:18,620 --> 00:03:22,600 +لنا stuff، العنوان تبع الـstuff الكوكاية، لحظة + +39 +00:03:22,600 --> 00:03:28,260 +إنها موجودة على background خضرة وفيها ثقوب، هي + +40 +00:03:28,260 --> 00:03:30,980 +عبارة عن membrane filter، الثقوب هذه عبارة عن + +41 +00:03:30,980 --> 00:03:32,900 +membrane filter، فاكرين ال membrane filter اللي + +42 +00:03:32,900 --> 00:03:36,880 +استخدمناه في عملية التنقية، في ال sterilization، + +43 +00:03:36,880 --> 00:03:40,890 +في ال physical removal من ال micro organismلما + +44 +00:03:40,890 --> 00:03:45,470 +قلنا إن المسامات بتكون أصغر عن حجم البكتيري، مش + +45 +00:03:45,470 --> 00:03:49,330 +هذه الفكرة اللي انا بدي أوضحها، بدي أوضح إن شكل + +46 +00:03:49,330 --> 00:03:53,530 +الـ Staphylococci بيعطيني إحاء بإن الإنقسامات + +47 +00:03:53,530 --> 00:03:58,910 +بتكون في كل الاتجاهاتممكن في هذا البُعد، في هذا + +48 +00:03:58,910 --> 00:04:03,570 +البُعد، البُعد الأسفل، الليمين إلى اليسار بينما لو + +49 +00:04:03,570 --> 00:04:07,630 +البكتيريا Striptococci هتكون الانقسام في اتجاه + +50 +00:04:07,630 --> 00:04:12,390 +واحد ما الذي يحدد طريقة الانقسام؟ أيضا هو ال + +51 +00:04:12,390 --> 00:04:17,230 +genetic makeup، يعني التكوين الوراثي للبكتيريا + +52 +00:04:19,680 --> 00:04:24,220 +morphology and identification للاستثمار اللي كوكا، + +53 +00:04:24,220 --> 00:04:27,360 +they are ground positive و هذه حقيقة خلاص هي ثابتة + +54 +00:04:27,360 --> 00:04:31,980 +الآن، يعني مافيش داعي نضل نكررها، بس أساسية في + +55 +00:04:31,980 --> 00:04:35,960 +التعرف، فكنت دي في أنا أيروب، يعني أنا مجانا نميها + +56 +00:04:35,960 --> 00:04:40,220 +هوايا ولا هوايا، grape-like clusters كعناقيد + +57 +00:04:40,220 --> 00:04:45,630 +لاقينا بشكلالبكتيريا تحت ال microscope كتاليز + +58 +00:04:45,630 --> 00:04:52,550 +بوستر وهذه صفة محورية ايه صفة محورية يعني احنا + +59 +00:04:52,550 --> 00:05:00,490 +هندرس تحت ال biogenic cocci to two genuses من + +60 +00:05:00,490 --> 00:05:03,030 +الجرام بوستر في كوكساية الاستفيل الكوكاية + +61 +00:05:03,030 --> 00:05:05,990 +والاستريبت الكوكاية يعني في يوم من الأيام لو انا + +62 +00:05:05,990 --> 00:05:08,850 +عزلت جرام بوستر في كوكساية فهي واحدة من تنتين + +63 +00:05:12,020 --> 00:05:15,700 +الصفر اللي بيبقى مميزة بينهم الكتاليز الستفيل + +64 +00:05:15,700 --> 00:05:18,600 +الكوكاية كتاليز بوستف بينما الستفيل الكوكاية + +65 +00:05:18,600 --> 00:05:21,900 +كتاليز نجلد هذه نقطة محورية كمان مرة في ال + +66 +00:05:21,900 --> 00:05:26,460 +diagnosis major component of normal flora of the + +67 +00:05:26,460 --> 00:05:30,440 +nose and skin وكلمة major component يعني هي جزء + +68 +00:05:30,440 --> 00:05:34,080 +أساسي أو مكون أساسي من ال normal flora في الأنفة + +69 +00:05:34,080 --> 00:05:38,260 +اللي هو ال الجلد المعلومات هذه مهمة عشان نحن نحن + +70 +00:05:38,260 --> 00:05:43,780 +نرجعلها أنها skin و noseهذه ايضا صورة بتوضح ان ال + +71 +00:05:43,780 --> 00:05:46,580 +.. ال .. اليسار ال gram-boster-cuxi مأخودة من + +72 +00:05:46,580 --> 00:05:49,800 +culture، يعني من مستعمرة، تمام؟ وبتاع ال .. ال + +73 +00:05:49,800 --> 00:05:52,800 +background تبعتها واضح، مافيش فيها اي شيء، بينما + +74 +00:05:52,800 --> 00:05:57,660 +الأخرى هذي، ايش هتحب تبتب اكتبت عليها؟ بس، دلوقت + +75 +00:05:57,660 --> 00:06:03,320 +انها مأخودة من عينة فيها صديق وصبغناها، الان هذا + +76 +00:06:03,320 --> 00:06:09,360 +المشكلةهل نستطيع بدقة و حرافية أن نميز الاستفادة + +77 +00:06:09,360 --> 00:06:12,240 +من الكوكاية عن الاسترابات من الكوكاية من خلال + +78 +00:06:12,240 --> 00:06:14,560 +الشكل اللي احنا بقوله هذيك grape-like clusters و + +79 +00:06:14,560 --> 00:06:19,880 +التانية سلاسة لكن في واقع الأمر لو خدنا عنقود عينة + +80 +00:06:19,880 --> 00:06:29,110 +و ضربناها في الحقق هيتفتتالى حبات منفصلة او حبتين + +81 +00:06:29,110 --> 00:06:32,550 +او حبتين ييجى فى جنب حبتين خدوا بالكوا الأشكال + +82 +00:06:32,550 --> 00:06:35,890 +اللى انا حاكيتهم مختلفين تماما حبه single cell + +83 +00:06:35,890 --> 00:06:40,290 +حبتين دبلو كوكاى تنتهوا تنتين سهروا stripped + +84 +00:06:40,290 --> 00:06:44,490 +الكوكاى اربع لو كانوا تكوّموا فى بعض دبلو كوكاى طب + +85 +00:06:44,490 --> 00:06:50,010 +انا كيف بدي اعرف ان المدولة انقود على فرفط انعقد + +86 +00:06:50,010 --> 00:06:56,400 +الحبات تبعته راحت ولا هو واقع الامر هيكفبدأ أستخدم + +87 +00:06:56,400 --> 00:07:00,620 +مين؟ الكتاليز إذا you cannot identify أو + +88 +00:07:00,620 --> 00:07:03,740 +differentiate Staphylococci from streptococci with + +89 +00:07:03,740 --> 00:07:07,080 +certainty unless you perform the test اللي اسمه + +90 +00:07:07,080 --> 00:07:12,180 +كتاليز و الكتاليز من أسهل الفحوصات بندرجه من ضمن + +91 +00:07:12,180 --> 00:07:15,880 +ال one minute test one minute test يعني يعني + +92 +00:07:15,880 --> 00:07:21,220 +بيستغلقش معانا دقيقة حتىهذه الصورة اللي أمامنا + +93 +00:07:21,220 --> 00:07:24,440 +بتوضح لنا اللي هي فكرة الفحص وهو عبارة عن قطرة من + +94 +00:07:24,440 --> 00:07:27,240 +hydrogen peroxide تلاتة في المية بنضيف عليها ال + +95 +00:07:27,240 --> 00:07:31,260 +micro organism إذا ال micro organism بيحتوي على + +96 +00:07:31,260 --> 00:07:36,920 +الكتاليز هيحلله ال hydrogen peroxide و oxygen الآن + +97 +00:07:36,920 --> 00:07:40,700 +ال oxygen تكونه ضمن ال water اللي هو ال hydrogen + +98 +00:07:40,700 --> 00:07:44,980 +peroxide فبتطلع بشكل فقعة خولوا بالكم من النقطة + +99 +00:07:44,980 --> 00:07:48,160 +كمان شوية هروح على step 2 kokai أو مش كمان شوية + +100 +00:07:48,160 --> 00:07:52,660 +المحاضرة اللي قادمةفحلاجيها catalase negative لكن + +101 +00:07:52,660 --> 00:07:57,200 +حلاجيها facultative anaerobic facultative + +102 +00:07:57,200 --> 00:08:03,440 +anaerobic مؤشر على أنه عندها mechanism by which + +103 +00:08:03,440 --> 00:08:11,220 +they can defend themselves against oxygen مثلا ال + +104 +00:08:11,220 --> 00:08:16,100 +catalase هنا هو المصبوب هو اللي حمانه من اثيرات ال + +105 +00:08:16,100 --> 00:08:20,080 +hydrogen peroxide طب هناك مين بيحميهامن الهيدرولين + +106 +00:08:20,080 --> 00:08:23,540 +بيروكسايد فاكرين يقولنا الأن دي متباعتنا معتمد على + +107 +00:08:23,540 --> 00:08:30,980 +super oxide بسم تاز وكتاليز او بيروكسدايز الان ال + +108 +00:08:30,980 --> 00:08:34,240 +striptococcus فيها بيروكسدايز مافيش فيها كتاليز + +109 +00:08:34,240 --> 00:08:35,860 +عشان يسمينا كتاليز نيجاتي + +110 +00:08:39,900 --> 00:08:42,620 +قبل ما ندخل في البعض Genesis و ال immunity ل ال + +111 +00:08:42,620 --> 00:08:46,600 +Staphylococcus species نظهر على ال cell wall و + +112 +00:08:46,600 --> 00:08:49,000 +نتذكر مع بعض بعض ال components اللي موجودة في ال + +113 +00:08:49,000 --> 00:08:52,920 +cell wall capsule or polysaccharide slime layer + +114 +00:08:52,920 --> 00:09:02,320 +ليش or؟ كلمة or معناها ان بعض السلالة تحتوي عليها + +115 +00:09:02,320 --> 00:09:06,640 +و سلالة أخرى تحتوي عليها كلمة سلالة بدنا نفهمها + +116 +00:09:06,640 --> 00:09:10,220 +اليوم لإن هو كتير و كتير مهمةانتوا طالبات سنة + +117 +00:09:10,220 --> 00:09:13,320 +تانية بيتعامل معاكم بهذا الأنوان انتوا طالبات سنة + +118 +00:09:13,320 --> 00:09:16,780 +تانية انتوا كلكوا واحد لكن ال .. ال .. ال .. ال .. + +119 +00:09:16,780 --> 00:09:17,160 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +120 +00:09:17,160 --> 00:09:18,420 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +121 +00:09:18,420 --> 00:09:18,860 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +122 +00:09:18,860 --> 00:09:19,580 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +123 +00:09:19,580 --> 00:09:19,660 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +124 +00:09:19,660 --> 00:09:20,440 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +125 +00:09:20,440 --> 00:09:29,320 +ال .. ال .. ال .. ال .. ال .. + +126 +00:09:29,320 --> 00:09:29,320 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +127 +00:09:29,320 --> 00:09:29,320 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +128 +00:09:29,320 --> 00:09:30,920 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +129 +00:09:30,920 --> 00:09:34,380 +.. ال .. ال + +130 +00:09:36,580 --> 00:09:39,820 +بس لازم يقطر على بالك مجموعة زي اللي رحجته طلاب + +131 +00:09:39,820 --> 00:09:43,760 +سنة تانية مع الحاجة إن أستافيل الكوكاس أورس ليش + +132 +00:09:43,760 --> 00:09:48,520 +فيها strains مختلفة هي species لكن فيها strains + +133 +00:09:48,520 --> 00:09:53,180 +مختلفة قد يحتوي ال strain على تكسين X و ال strain + +134 +00:09:53,180 --> 00:09:56,660 +بتاعي ليه يحتوي على هذا التكسين لكن لما أنا بدي + +135 +00:09:56,660 --> 00:10:00,520 +بدرسهم إلكوا و بحطوكوا كل ال toxins اللي في كل ال + +136 +00:10:00,520 --> 00:10:05,850 +strains ولا لأ؟ طيببيبتدش lie can layer إيه أهمية + +137 +00:10:05,850 --> 00:10:11,030 +بالنسبة للبعض نفسه اللي مانوش أهمية؟ إيش أهميته في + +138 +00:10:11,030 --> 00:10:14,810 +البعض نفسه؟ لما اتحدثنا عن ال gram negative بس ال + +139 +00:10:14,810 --> 00:10:20,690 +lie، اتحدثت انا عن حاجة اسمها gram negative ليبو + +140 +00:10:20,690 --> 00:10:24,210 +بوليسا كريت و اتحدثنا عن مصائب ليبو بوليسا كريت و + +141 +00:10:24,210 --> 00:10:29,060 +سئلنا سؤال بريء، هل هناكفي الاجراءة مبسطة في الـ + +142 +00:10:29,060 --> 00:10:32,760 +counter part اللي اللي يقول سكراي و الجواب نعم و + +143 +00:10:32,760 --> 00:10:37,600 +اللي هو اللي بيقليده is likeإذن إيه أهمية في + +144 +00:10:37,600 --> 00:10:41,100 +الفعطة الجنسية للستافيلو كوكاس؟ في هذا التيكويك + +145 +00:10:41,100 --> 00:10:44,840 +أسد كنا قلنا أيضا إله أهمية في الفعطة الجنسية و + +146 +00:10:44,840 --> 00:10:50,560 +البروتين E اللي كان يمسك ال Antibody كنا قلنا + +147 +00:10:50,560 --> 00:10:53,700 +عملينه two antigen binding site و من تحت اللي هو + +148 +00:10:53,700 --> 00:10:56,700 +ال Fc portion كان بدل من ارتبط زي هيك في + +149 +00:10:56,700 --> 00:10:59,480 +البكتيريا، البكتيريا تربطه من تحت، هذا البروتين E + +150 +00:10:59,480 --> 00:11:02,800 +كنا قلنا عنه من أعظم ال viral instructors في + +151 +00:11:02,800 --> 00:11:08,610 +الستافيلو كوكاسفينا طبعا الـ cell membrane ده + +152 +00:11:08,610 --> 00:11:11,790 +component طبيعي موجودة، ال clamping factor او ما + +153 +00:11:11,790 --> 00:11:17,190 +يسمى بال coagulase و مارو معانا ذكره اللي بيحول ال + +154 +00:11:17,190 --> 00:11:23,000 +fibrinogen إلى fibrinهذا الشكل دراساناه وشوفناه هو + +155 +00:11:23,000 --> 00:11:26,260 +ال protein A بقولنا انه بيرتبط ال .. ال .. هي ال + +156 +00:11:26,260 --> 00:11:29,640 +immunoglobulin و هو ال protein A بيرتبط منين؟ + +157 +00:11:29,640 --> 00:11:33,300 +بيرتبط بال Fc portion و ال Fc portion هذا مخصص + +158 +00:11:33,300 --> 00:11:36,600 +للارتباط بال macro phage و بال phagocyte so + +159 +00:11:36,600 --> 00:11:39,700 +بالتالي ال macro phage و ال phagocyte لن تجد شيء + +160 +00:11:39,700 --> 00:11:43,820 +ترتبط فيه وبالتالي لن تستطيع الانتقام للبكتيريا + +161 +00:11:43,820 --> 00:11:48,010 +ليها Staphylococcus aureusمن بعض ال toxins و ال + +162 +00:11:48,010 --> 00:11:52,150 +enzymes اللي متوفرة في حضرة ال Staphylococci و + +163 +00:11:52,150 --> 00:11:56,370 +Aureus بالذات ال Catalase و يعرفنا إيش الأهمية + +164 +00:11:56,370 --> 00:11:59,550 +تبعته ال Coagulase ماعرفناش الأهمية .. أنا + +165 +00:11:59,550 --> 00:12:03,930 +قولتلكوا و نزلتكوا فيديو على الصفحة اسمه virulence + +166 +00:12:03,930 --> 00:12:08,230 +factors of staphylococcus aureus هرجعوا لحضراتكم + +167 +00:12:08,230 --> 00:12:12,790 +إنكوا تطلعوا عليه و عشر دقائق أقل من عشر دقائق + +168 +00:12:12,790 --> 00:12:18,380 +تسعة أو اشهر تمام يعني هم بتطلعوا عليهو تشوفوا ال + +169 +00:12:18,380 --> 00:12:21,120 +.. ال .. ال .. ال violence factor وقتش بيلزم + +170 +00:12:21,120 --> 00:12:25,520 +البكتيريا من دخولها لحد وصولها للدمخ و كيف ال + +171 +00:12:25,520 --> 00:12:29,320 +coagulase بيساعدها كيف بيساعدها ال .. ال .. ال .. + +172 +00:12:29,320 --> 00:12:31,780 +ال .. ال hyaluridase على سبيل المثال كل هذه + +173 +00:12:31,780 --> 00:12:36,180 +الأشياء من .. يعني موجودة في هذا البيت catalase, + +174 +00:12:36,300 --> 00:12:40,440 +coagulase, hyaluridase and lipase hemolysin أو + +175 +00:12:40,440 --> 00:12:44,080 +حاجة بيسموها sphingomyelinase-c و كل .. كل كنت + +176 +00:12:44,080 --> 00:12:49,620 +بتعرف هيموليسين يعنيToxin that will disrupt the + +177 +00:12:49,620 --> 00:12:51,960 +cell membrane of red blood cell و بيعملها + +178 +00:12:51,960 --> 00:12:55,260 +destruction كيف كهو؟ بيعمل destruction ل red blood + +179 +00:12:55,260 --> 00:12:59,120 +cell ل cell membrane و بدلا بيصير lysis كلمة lysis + +180 +00:12:59,120 --> 00:13:04,280 +لدم hemolysis همولايسيز تبعها هذا مؤشر أن + +181 +00:13:04,280 --> 00:13:07,700 +الستافيلو كوكاس أوريس اللي بتمتلك هذه ال toxin لو + +182 +00:13:07,700 --> 00:13:11,380 +زرعناها ع بلد أجارها بنات هتعمل hemolysis حول + +183 +00:13:11,380 --> 00:13:16,620 +المستعمرة تبعهاو ال hemolysis صفة من الصفات المهمة + +184 +00:13:16,620 --> 00:13:21,100 +جدا اللي نستخدمها بالتعرف على البكتير Exfoliative + +185 +00:13:21,100 --> 00:13:27,040 +toxin أول مرة شفتوا طفل بتجشر أطفال الجيل تبعه + +186 +00:13:27,040 --> 00:13:34,210 +بتجشر، ماشفتوش؟ بتجشروا؟ أه ده طبيعيلكن لما يكون + +187 +00:13:34,210 --> 00:13:38,950 +او بلاش اطفال راحوا على البحر و من الشمس طبعا + +188 +00:13:38,950 --> 00:13:43,710 +صاروا طبقات من الجلد يمكن نزعها من هؤلاء الأطفال + +189 +00:13:43,710 --> 00:13:47,450 +او حتى من الأكبر ال Exfoliative toxin اللي + +190 +00:13:47,450 --> 00:13:50,590 +البكتيريا عملت infection لهذا الإنسان بصير جلده + +191 +00:13:50,590 --> 00:13:55,770 +يتقشر كما و انه بالفعل يعني قشرة خارجي toxic shock + +192 +00:13:55,770 --> 00:14:01,700 +syndrome توكسين وبينبوسين super antigenو ال .. ال + +193 +00:14:01,700 --> 00:14:07,420 +enterotoxin انا بتركز على اخر اتنين و اخد منهم اخر + +194 +00:14:07,420 --> 00:14:14,260 +واحد ال enterotoxin ايش معنى entero؟ معوي لأ لك + +195 +00:14:14,260 --> 00:14:18,900 +اندو entero enteric معوي و احنا حاكيها تقولنا انه + +196 +00:14:18,900 --> 00:14:22,720 +تصنف ال toxins بحسب ال site ال euro او ال terro + +197 +00:14:22,720 --> 00:14:28,200 +اذا انا مابشتل هذا؟ في ال intestine طيب اسمعوا هل + +198 +00:14:28,200 --> 00:14:31,730 +الكلام موضوع حد مبسوط عليه خالص؟اللي هو علاقة + +199 +00:14:31,730 --> 00:14:36,110 +بالمفتول؟ أظن أن كلكم بتعرفوا المفتول و لا بتعرفوش + +200 +00:14:36,110 --> 00:14:41,510 +المفتول و لا ليهم برضه من فرنسا من غزة هذكركم + +201 +00:14:41,510 --> 00:14:44,770 +الخطوات اللي .. اللي .. المعمول بيها بالمفتول عشان + +202 +00:14:44,770 --> 00:14:50,010 +نعرف .. عشان نعرف وين .. وين الخفق ممكن يحدث انتوا + +203 +00:14:50,010 --> 00:14:54,630 +بتاخدوا دقيق بتخلطوا بالماء و بعدين بتعملوا كوريات + +204 +00:14:54,630 --> 00:15:00,410 +عبر ضغطه في شبكةو من ثم تاخدوا هذه الكوريات و + +205 +00:15:00,410 --> 00:15:05,450 +تحطوها في وعاء مثقب و تحطوها على ماء او whatsoever + +206 +00:15:05,450 --> 00:15:10,870 +سائل و بتغلو السائل فالسائل بخار تبقى و بدخل عبر + +207 +00:15:10,870 --> 00:15:16,970 +المفتول و بينضجه بينضجه يعني بيصل حرارته الى حرارة + +208 +00:15:16,970 --> 00:15:20,410 +عالية جدا لو كان فيه مايكروبات من اي نوع غالبا + +209 +00:15:20,410 --> 00:15:24,830 +هتموت في العملية اللي هو ال cookingخلصنا من النقطة + +210 +00:15:24,830 --> 00:15:30,050 +دي، الأن نزلنا و كبنا، مش هيك صح الطريقة؟ بيكون كل + +211 +00:15:30,050 --> 00:15:33,830 +واحدة مفلوطة عن بعضها ولا مكتل؟ مكتل .. مكتل .. + +212 +00:15:33,830 --> 00:15:36,170 +مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. + +213 +00:15:36,170 --> 00:15:36,210 +مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. + +214 +00:15:36,210 --> 00:15:38,150 +مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. + +215 +00:15:38,150 --> 00:15:39,130 +مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. + +216 +00:15:39,130 --> 00:15:47,050 +مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. مكتل .. + +217 +00:15:47,050 --> 00:15:54,350 +مكتل .. مكتل ..زي المفتول اللي طبخناه قبل شوية + +218 +00:15:54,350 --> 00:15:59,490 +واخدنا لوب و حطناه في عينا و زراعنا البكتيريا + +219 +00:15:59,490 --> 00:16:03,290 +أصابعنا هم اللوب تمام و أظافرنا هم البكتيريا + +220 +00:16:03,290 --> 00:16:07,090 +حطناهم في المفتول و زرعنا المفتول، الآن بكتيريا، + +221 +00:16:07,090 --> 00:16:12,960 +كويس؟ خدنا بالكم النقطتينالنقطة الأولى إن هذا + +222 +00:16:12,960 --> 00:16:17,520 +معقّم و أضفنا له بكتيريا واحدة من نوع واحد و ذكرت + +223 +00:16:17,520 --> 00:16:23,140 +أنا مكان البكتيريا قبل قليل في الأنف وين؟ في الجلد + +224 +00:16:23,140 --> 00:16:28,220 +فبالتالي احنا زرعنا البكتيريا في مكان معقّم، إيش + +225 +00:16:28,220 --> 00:16:30,700 +يعني كلمة مكان معقّم؟ يعني لو مش معقّم أحسن، اه لو + +226 +00:16:30,700 --> 00:16:33,440 +مش معقّم أحسنفاكرين لما اتكلمنا عن normal + +227 +00:16:33,440 --> 00:16:37,960 +microbiota؟ اتكلمنا عن انه من صفاتها انها بتعمل + +228 +00:16:37,960 --> 00:16:41,580 +اتزان بينها وبين بعض، لإن في منها منافسة، بس ال + +229 +00:16:41,580 --> 00:16:45,300 +Staphylococcus aureus الآن، هل لها منافس؟ مالاش + +230 +00:16:45,300 --> 00:16:49,020 +منافس، هذا نقطة، نقطة تانية، الدقيق لما كان + +231 +00:16:49,020 --> 00:16:53,020 +uncooked، يعني غير مطبوخ، غير مطهي، ما كان فيه + +232 +00:16:53,020 --> 00:16:56,940 +لطوبة أصلا جافة، وبالتالي كان غير صارح لمين؟ لنمو + +233 +00:16:56,940 --> 00:17:01,110 +البكتيرا، الآن فيه ماءفي ال .. في ال .. في الغذاء + +234 +00:17:01,110 --> 00:17:06,130 +و أصبح ايه .. ملائم و خاصة ان بعض ال nutrients + +235 +00:17:06,130 --> 00:17:10,810 +نزلت من الخلايا و صارت موجودة الآن متاحة للبكتيريا + +236 +00:17:10,810 --> 00:17:18,050 +فخلال 6 إلى 8 ساعات ابتنموا البكتيريا إلى أعداد + +237 +00:17:18,050 --> 00:17:21,990 +quorum sensing إذا وصلت البكتيريا لquorum sensing + +238 +00:17:21,990 --> 00:17:25,150 +عشرة لقص خمسة لكل gram تبدأ تنتج ال toxin تبعها + +239 +00:17:25,150 --> 00:17:28,390 +اللي هو ال enterotoxinطبعا احنا بنعمل .. بنعمل + +240 +00:17:28,390 --> 00:17:31,850 +مفتول كأكل شعبية، بنعملش للغدا و بس، بنعمل للغدا و + +241 +00:17:31,850 --> 00:17:36,890 +للعشاء و بنوزع الجيران و على الحباب .. المساء جينا + +242 +00:17:36,890 --> 00:17:41,010 +سخنا المفتول، بدنا نتعشى من المفتول، بعد ساعتين + +243 +00:17:41,010 --> 00:17:44,870 +صارنا مغص، vomiting اعرف ان انت صار عندك مفتولهيتس + +244 +00:17:44,870 --> 00:17:49,410 +اللي هي food poisoning .. food poisoning .. food + +245 +00:17:49,410 --> 00:17:52,810 +poisoning .. تسموه من غذائي من ال Staphylococcus + +246 +00:17:52,810 --> 00:17:53,570 +aureus + +247 +00:17:59,010 --> 00:18:04,070 +بنقف عندها جئماليا انا قولت سخننا الأكل سخننا + +248 +00:18:04,070 --> 00:18:09,090 +الغذاء هذا بتدل على انه لو البكتيريا ماتت مش انا + +249 +00:18:09,090 --> 00:18:12,690 +مش هي المشكلة اللي عندي انا عندي ال toxin و ال + +250 +00:18:12,690 --> 00:18:17,750 +toxin heat stable يعني لو سخننا و غلينا هضله زي ما + +251 +00:18:17,750 --> 00:18:23,470 +هو هو الوحيد اللي heat stable من كل ال x-toxin ايش + +252 +00:18:23,470 --> 00:18:27,860 +رأيكم؟ ال foodpoisoning enterotoxin تبع + +253 +00:18:27,860 --> 00:18:30,900 +الcephalococcus aureus هو الوحيد اللي هو heat + +254 +00:18:30,900 --> 00:18:36,320 +stable من كل ال exotoxins استفدنا من المعلومة هذه + +255 +00:18:36,320 --> 00:18:39,000 +مجموعة معلومة تاخدوا بالك وانا القصة مش هي اللي + +256 +00:18:39,000 --> 00:18:43,420 +بتعنيني قصة انه انا عندي ال post cooking + +257 +00:18:43,420 --> 00:18:49,450 +contamination اخطر من ال pre cookingعندي مشكلة + +258 +00:18:49,450 --> 00:18:53,950 +تانية انه كونه ال .. ال .. ال .. ال .. ال toxin + +259 +00:18:53,950 --> 00:18:58,210 +اتكون داخل الغذاء معناه اللي حاكي انه انا لم احفظه + +260 +00:18:58,210 --> 00:19:01,670 +على temperature غير ملائمة لنمو ال micro organism + +261 +00:19:01,670 --> 00:19:05,570 +وبالتالي هنغير نظرتنا على موضوع تخزين الأكل اللي + +262 +00:19:05,570 --> 00:19:09,050 +من ضمنها الأكل الشعبية المعروفة بالمفطول انا ذكرت + +263 +00:19:09,050 --> 00:19:13,980 +المفطول خد بالكوا كنموذجبس هناك أغذية كثيرة قد + +264 +00:19:13,980 --> 00:19:17,340 +يحدث بيها نفس ال .. اعطاكم مثال تاني تاعة ال cake + +265 +00:19:17,340 --> 00:19:20,640 +اللي بتفور و الأشياء اللي دلوقت مرات في الاجتماعات + +266 +00:19:20,640 --> 00:19:26,940 +احنا بيجيبوننا عجزاة عصير و قطعة cake احد المرات + +267 +00:19:26,940 --> 00:19:32,480 +انا شخصيا عندي سكر يعني فمش كتير مغرم لكن يبدو اني + +268 +00:19:32,480 --> 00:19:36,500 +كنت جاعان فأكلت .. متكلم مع اللي جان فأكلت يعنيو + +269 +00:19:36,500 --> 00:19:40,040 +بعد بالفعل أقل من ساعتين كل الأراضي اللى هتكلم + +270 +00:19:40,040 --> 00:19:44,000 +عنها بالنسبة لها كانت موجودة دلوقت لأنه برضه هو + +271 +00:19:44,000 --> 00:19:48,240 +food poisoning لكن + +272 +00:19:48,240 --> 00:19:51,420 +لو جينا على المستشفيات و على ال medical records و + +273 +00:19:51,420 --> 00:19:55,740 +عدينا أكم حالة مسجلة عندهم مشخصة رأينا food + +274 +00:19:55,740 --> 00:19:57,720 +poisoning بال food poisoning سأولياس هلاجيها zero + +275 +00:19:59,980 --> 00:20:03,640 +أول حاجة ال onset اللي هي ال disease نفسه بيستغرق + +276 +00:20:03,640 --> 00:20:08,480 +ساعات بسيطة و self-limited ايش يعني self-limited؟ + +277 +00:20:08,480 --> 00:20:12,640 +يعني لحاله و ال resolve فاحنا ماعطيهوش أهمية كبيرة + +278 +00:20:12,640 --> 00:20:19,400 +لكن تخيلوا كتاب حضرتك 6 الصبح او الظهر whatsoever + +279 +00:20:19,400 --> 00:20:21,860 +المواعد اللي غيرتي فيه و بعد ساعتين صار عندك اللي + +280 +00:20:21,860 --> 00:20:25,600 +هو gastroenteritis و عندك امتحان او عندك جامعة او + +281 +00:20:25,600 --> 00:20:30,720 +عندك lecture محاضرة انت مش هتروح على هذا المكانفي + +282 +00:20:30,720 --> 00:20:34,080 +خلال فترة ال .. ال .. ال onset لن تستطيعوا بتالي + +283 +00:20:34,080 --> 00:20:38,480 +لو جينا حسبنا التكلفة الاقتصادية الناجمة عن ال + +284 +00:20:38,480 --> 00:20:41,200 +food poisoning بستفيل الكوكس أو الهلجيهة ضخمة جدا + +285 +00:20:41,200 --> 00:20:43,940 +تخيلوا ان الوضع صار معايا أنا و غيبت انا عن + +286 +00:20:43,940 --> 00:20:47,780 +المحاضرة هذه او غيبت اليوم كله عن المحاضرات اكم + +287 +00:20:47,780 --> 00:20:51,420 +طالبة عندى ستين طالبة ستين في مواصلاتهم اللى اجوا + +288 +00:20:51,420 --> 00:20:54,600 +فيهم و الساعة الزمنية اللى استغلقوها مابتكلم عن .. + +289 +00:20:54,600 --> 00:20:59,110 +انا .. انا فلوسهذا كله كان مخسر و مش مخسر، + +290 +00:20:59,110 --> 00:21:02,510 +بالإضافة إنه ممكن أنا أروح على الإيادة و أدفع ثمن + +291 +00:21:02,510 --> 00:21:06,510 +علاج و هم يعطوني علاج، أنام في المستشفى، فالتكلفة + +292 +00:21:06,510 --> 00:21:10,470 +الاقتصادية لإنه بتكلم عن food و ال food غالبا هو + +293 +00:21:10,470 --> 00:21:14,130 +social occasion، إيش أنا social occasion؟يعني وضع + +294 +00:21:14,130 --> 00:21:17,810 +اجتماعي، مابناكلش لحالنا بالغالب، مابناكل مع أفراد + +295 +00:21:17,810 --> 00:21:21,390 +أخرين صح أو لا؟ وبالتالي لو صار فيه food poisoning + +296 +00:21:21,390 --> 00:21:25,490 +نسمعش عن شخص واحد، نسمع عنه .. عن مجموعة كبيرة من + +297 +00:21:25,490 --> 00:21:29,230 +الناس، لا قدر الله احنا في .. في .. في جامعة و + +298 +00:21:29,230 --> 00:21:34,030 +الكفتيريا صار فيها غذاء ملوى و من العشر تلاف طالبة + +299 +00:21:34,030 --> 00:21:38,470 +تلت تلاف كانوا وسط الكفتيريا و أكل من هذا الغذاء + +300 +00:21:39,360 --> 00:21:42,580 +أنا بحاجة كده بتكلم عن مصائب ولا لأ ف ال food + +301 +00:21:42,580 --> 00:21:46,040 +poisoning اللي .. اللي .. اللي بتسبب استفادة + +302 +00:21:46,040 --> 00:21:49,920 +الكوكس أولي الصحيح mild لكن بيفتح أمامنا أفاق أنه + +303 +00:21:49,920 --> 00:21:52,280 +في حد اسمه food poisoning و ال food poisoning + +304 +00:21:52,280 --> 00:21:56,020 +بيختلف عن ال food infection ال food infection هو + +305 +00:21:56,020 --> 00:21:58,880 +أنه أنا عايز أناور الغذاء و فيه الميكروب و + +306 +00:21:58,880 --> 00:22:03,860 +الميكروب يدخل جوا و يعمل تكسين جوا بينما ال food + +307 +00:22:03,860 --> 00:22:07,730 +poisoning التكسين وانصارExternally في الغداء نفسه، + +308 +00:22:07,730 --> 00:22:10,210 +طب ما هذا نقطة كتير مهمة نجي للي جابله، هذا اللي + +309 +00:22:10,210 --> 00:22:13,710 +جابله محرج شوية اللي هو toxic shock syndrome toxin + +310 +00:22:13,710 --> 00:22:20,090 +لكن لابد أن نتطرقله بشكل جيد الفتيات عند البلوغ + +311 +00:22:20,090 --> 00:22:23,990 +بيصبح عندهم الحالة المعروفة بالدورة الشهرية، + +312 +00:22:23,990 --> 00:22:29,190 +مظبوط؟ و طبعا البنت بتخرج أو الست بتخرج إلى العمل + +313 +00:22:29,190 --> 00:22:34,710 +فبالتالي بتستخدم ما يسمى بالحفاضات النسائيةالحفظات + +314 +00:22:34,710 --> 00:22:40,130 +النسائية الآن business رائج كتير على كوكب الأرض من + +315 +00:22:40,130 --> 00:22:44,930 +الستات وبالتالي في استهلاك فالشركات تتنافس مين + +316 +00:22:44,930 --> 00:22:50,850 +ينتج أفضل أريح واللي أهم من ذلك اللي بيطول أكتر + +317 +00:22:51,670 --> 00:22:54,890 +most absorbent اللي بتمتص كمية أكبر من الدم + +318 +00:22:54,890 --> 00:22:59,350 +أذكركوا الدم ضفناه على ال media في المعمل عشان + +319 +00:22:59,350 --> 00:23:03,030 +نعمل blood agar ليش ضفنا ال blood؟ to enrich the + +320 +00:23:03,030 --> 00:23:07,910 +media معناه الحاجة أن الدم غاني جدا بالغدا فكمية + +321 +00:23:07,910 --> 00:23:11,050 +الدم كبيرة موجودة دقان في ال .. في ال .. في ال + +322 +00:23:11,050 --> 00:23:14,990 +tampons تمام؟ و الcephalococcus aureus موجودة على + +323 +00:23:14,990 --> 00:23:19,570 +الجلدهتنمو البكتيريا فى الدم و تصل كورام سينسينج + +324 +00:23:19,570 --> 00:23:22,990 +وتنتج ال super antigen تبعها اللى هو ال toxic + +325 +00:23:22,990 --> 00:23:29,150 +shock syndrome toxin أو TSSD that will be absorbed + +326 +00:23:29,150 --> 00:23:32,590 +through the vagina of the mucous membrane.الان + +327 +00:23:32,590 --> 00:23:36,730 +فاكرين كلمة super antigen كيف بقى بتشتغل؟ هذكركوا + +328 +00:23:39,250 --> 00:23:43,310 +اللوحة اللي قدامنا هذه بتوضح لنا ان ال T-cell + +329 +00:23:43,310 --> 00:23:46,270 +ترتبط مع ال macrophage ال antigen presenting cell + +330 +00:23:46,270 --> 00:23:50,310 +specifically على ال antigen وانا قولتلكوا انه .. + +331 +00:23:50,310 --> 00:23:55,190 +شايفين الإشارة اللي موجودة على ال ..لما يكون فيه + +332 +00:23:55,190 --> 00:23:57,510 +لسه trial بيشوفوا بعض انه والله المناسبين مش + +333 +00:23:57,510 --> 00:24:00,830 +المناسبين هو بروح بربطهم مع بعض فبيعمل non + +334 +00:24:00,830 --> 00:24:04,810 +specific activation .. non specific activation لل + +335 +00:24:04,810 --> 00:24:08,130 +T cells إذا عمل non specific activation لل T cells + +336 +00:24:08,130 --> 00:24:14,170 +هيكون عندي ردة فاعل منعية مش طبيعية exaggerated و + +337 +00:24:14,170 --> 00:24:17,330 +بصير فيها مجموعة من الأعراض ممكن اتكلم عنهم بيجي + +338 +00:24:17,330 --> 00:24:20,470 +بس fever + +339 +00:24:22,820 --> 00:24:25,140 +يجب أن نركز على اللوحة لأنها برضه فيها بعض + +340 +00:24:25,140 --> 00:24:29,300 +التعبيرات هذه الكرتون مهم جدا ان الاتنين يلزقون مع + +341 +00:24:29,300 --> 00:24:32,020 +بعض يعني ال T-cell و ال antigen-presenting cell + +342 +00:24:32,020 --> 00:24:34,460 +وهو بتاع اللي بيعمل non specific activation لكن + +343 +00:24:34,460 --> 00:24:37,920 +بينتج على هذا disease fever, rashes, desquamation + +344 +00:24:37,920 --> 00:24:41,640 +وdesquamation يعني برضه موت في خلايا الجلب, + +345 +00:24:42,280 --> 00:24:46,300 +vomiting, diarrhea و myelias myelias اللي هي ألم + +346 +00:24:46,300 --> 00:24:50,180 +في العضلات ألم في العضلات طبعا انا بتوقع انه كونه + +347 +00:24:50,180 --> 00:24:54,980 +ال toxinومطص عبر الميوكاس ممبراني بجراع الدم ومدى + +348 +00:24:54,980 --> 00:25:00,520 +مجراع الدم الأعراض هتكون إيه؟ Systemic خد بالك ال + +349 +00:25:00,520 --> 00:25:01,860 +.. ال stuff اللي الكوكاس + +350 +00:25:07,590 --> 00:25:10,750 +locally in the intestine عشانك الأعراض وين كانت؟ + +351 +00:25:10,750 --> 00:25:14,770 +في ال intestinal tract، دياريا زاد vomiting، بينما + +352 +00:25:14,770 --> 00:25:19,890 +ال super antigen أو ال toxic shock syndrome تكسين + +353 +00:25:19,890 --> 00:25:22,750 +كان super antigen وين موجود في الدم وبالتالي + +354 +00:25:22,750 --> 00:25:26,150 +الأعراض كانت ايه؟ systemطبعا الصورة اللي قدامنا + +355 +00:25:26,150 --> 00:25:31,930 +هنا هي box ل تمبونز و cartoon ل .. اللي هو قولنا + +356 +00:25:31,930 --> 00:25:35,570 +ال super antigen كيف بيربط الخلايا بعض البعض و + +357 +00:25:35,570 --> 00:25:39,950 +الأخرى هي T-cell مع antigen presenting cell برضه + +358 +00:25:39,950 --> 00:25:43,110 +باين مراجعة سريعة على اللوحة catalase, coagulase, + +359 +00:25:43,330 --> 00:25:45,810 +hyaluronidase and lipase, hemolysine or + +360 +00:25:45,810 --> 00:25:49,090 +sphingomyelinase C و lyococidine exfoliative + +361 +00:25:49,090 --> 00:25:54,320 +toxin, toxic shock syndrome toxin و enterotoxinهذه + +362 +00:25:54,320 --> 00:26:00,900 +Staphylococcus aureus لما أنا بتكلم عن بكتيريا + +363 +00:26:00,900 --> 00:26:05,020 +موجودة على الإدان و مورودة في الأنف تتوقعوا أنها + +364 +00:26:05,020 --> 00:26:09,060 +تسبب لنا أمراض كثيرة و لا ماتسببلنا أمراض كثيرة؟ + +365 +00:26:09,060 --> 00:26:14,980 +لو حكينا الجلد تبعنا، شعرنا يتحكى لو حكيناه، ماحنا + +366 +00:26:14,980 --> 00:26:17,540 +ممكن نخدش الجلد و ماحنا مش شايفين أننا خدشنا الجلد + +367 +00:26:17,540 --> 00:26:21,080 +اندخل Staphylococcus aureus في بوصيلة الشعر، + +368 +00:26:21,080 --> 00:26:25,590 +هتعملنا infection هناكل الدمامل أو 90% من الدمامل + +369 +00:26:25,590 --> 00:26:29,430 +والخراجات اللى بتصيب الناس اللى فى المجتمع mostly + +370 +00:26:29,430 --> 00:26:33,050 +staphylococcus aureus staphylococcus aureus + +371 +00:26:33,050 --> 00:26:38,750 +المكورات الأنقوضية الذهبية اسمها جذاب لكن واقع + +372 +00:26:38,750 --> 00:26:42,910 +الأمر هي من أخطر أنواع الميكروبات وبالتالي يجب أن + +373 +00:26:42,910 --> 00:26:46,070 +تتوقعوا فى ال clinical practice فى ال medical + +374 +00:26:46,070 --> 00:26:51,200 +laboratory انكوا تعزلوا staphylococcus aureusطار + +375 +00:26:51,200 --> 00:26:55,000 +من أي ميكروب أخر من صنف الـ Gram-bostev bacteria + +376 +00:26:55,000 --> 00:26:57,940 +يبقى الـ Staphylococcus aureus هتكون number one ال + +377 +00:26:57,940 --> 00:27:04,060 +super star تبع ال Gram-bostev coccine من + +378 +00:27:04,060 --> 00:27:08,580 +ال diseases الفرنكلز وهي قبعا دماغ من .. يعني + +379 +00:27:08,580 --> 00:27:12,040 +قريبة من سطح الجلد دماغ من الخروجات .. قريبة من + +380 +00:27:12,040 --> 00:27:17,540 +سطح الجلد Stye اللي هو التهاب بوصيلة الشعر اللي في + +381 +00:27:17,540 --> 00:27:21,200 +العين اللي بالعامية بتلقاها الشحاتسنعرفها إنه شحات + +382 +00:27:21,200 --> 00:27:23,720 +يعني حبة في ال .. في ال .. في بوصية شعر العين + +383 +00:27:23,720 --> 00:27:29,020 +impetigo اسمها الحصف وحنشوف صور واقلها كمان شوية + +384 +00:27:29,020 --> 00:27:33,840 +معظم أطفالنا أصيبوا فيها في يوم من الأيام very + +385 +00:27:33,840 --> 00:27:37,920 +contagious معني جدا إذا كان طفل في المنزل مصاب + +386 +00:27:37,920 --> 00:27:40,560 +غالبا الأطفال كلهم اللي موجودين بيصابوا وغالبا + +387 +00:27:40,560 --> 00:27:45,210 +اللي كبار immune ل .. ل .. ل هذه ال bacteriaالـ + +388 +00:27:45,210 --> 00:27:48,370 +Epidermal Necrolysis والـ Exfoliative Dermatitis + +389 +00:27:48,370 --> 00:27:51,450 +بيشبهوا بعض فيه تنتين فيهم موت في خلايا الجلب + +390 +00:27:51,450 --> 00:27:55,910 +مستاكس التهاب الثدي أو التهاب الضرع عند الحيوانات + +391 +00:27:55,910 --> 00:28:01,290 +تمام ال abscess هو عبارة عن دُمل بس deep inside زي + +392 +00:28:01,290 --> 00:28:06,590 +ما يقول brain abscess liver abscess تمام هذا بيكون + +393 +00:28:06,590 --> 00:28:13,000 +وين؟ داخلبالنسبة لها ده على الجلد، في الجلد الحبوب + +394 +00:28:13,000 --> 00:28:16,860 +اللي بتطلع على الناس اللي بحلجوا دجنوا مثلا بتطلع + +395 +00:28:16,860 --> 00:28:20,160 +الحبوب تاني يوم أو في نفس اليوم غالبا في الكوكاس + +396 +00:28:20,160 --> 00:28:24,320 +أو جسد يعني بيوصفوها بإنها it causes infection + +397 +00:28:24,320 --> 00:28:28,500 +from head to toe من أعلى الرأس لأسفل القدم نظرا + +398 +00:28:28,500 --> 00:28:32,100 +لانتشارها على ال .. على الجلد اللي هو أكبر عضو في + +399 +00:28:32,100 --> 00:28:36,700 +جسم الإنسان اللي هو أيضا عرضة للجروح و الإصابات + +400 +00:28:36,700 --> 00:28:40,710 +بسبب احتكاكه مع البيئة الخارجةمن ال systemic + +401 +00:28:40,710 --> 00:28:45,370 +diseases بكتيريميا و غالبا بتكون من abscess او + +402 +00:28:45,370 --> 00:28:49,490 +wound او burn wounds اللي هي الجروح و ال burns + +403 +00:28:49,490 --> 00:28:52,230 +اللي هي الطلوق و الخروجات او ال dynamite زي ما + +404 +00:28:52,230 --> 00:28:56,530 +حكينا بكتيريميا اذكركوا بس ب .. ببعض ال .. كل + +405 +00:28:56,530 --> 00:29:01,270 +كنتوا أصغار طلالكوا حد في الوجه و طبعا كانت نهاية + +406 +00:29:01,270 --> 00:29:04,850 +العالم انه كيف يعني حد بطل ع جاية و لا بال .. + +407 +00:29:04,850 --> 00:29:11,230 +فبننزل نحاول ن .. ن .. نزيل أعطارهالما .. لأ و + +408 +00:29:11,230 --> 00:29:17,710 +انتوا كبار افهم انا .. انا ناسر الان ال .. ال .. + +409 +00:29:17,710 --> 00:29:22,510 +الحب هذه اللي احنا حاولنا نفرغ محتواهها غالبا + +410 +00:29:22,510 --> 00:29:26,230 +بنضغط عليها بأصابعنا وبالتالي اخر مرحلة بيكون نزول + +411 +00:29:26,230 --> 00:29:30,110 +دم معاها نزول دم يعني ذلك ان احنا كسرنا ال + +412 +00:29:30,110 --> 00:29:33,050 +capillaries في المنطقة كسرنا ال capillaries يعني + +413 +00:29:33,050 --> 00:29:37,980 +دخلنا البكتيريا داخل الدمفقد تنتقل من مكانها الى + +414 +00:29:37,980 --> 00:29:42,580 +اماكن اخرى بدل حب بصير لنا حبوب او الحب نفسه تكبر + +415 +00:29:42,580 --> 00:29:45,840 +او انه تنتقل الى الدم و تعملنا سكتسيميا و + +416 +00:29:45,840 --> 00:29:50,100 +بكتيريميا و تعملنا مشكلة كبيرة اذا ما حاولش انه + +417 +00:29:50,100 --> 00:29:54,060 +نتخلص من الحبوب بيها نفسنا على الرغم من ان واحد من + +418 +00:29:54,060 --> 00:29:59,700 +طرق العلاج هو to drain .. drain يعني سحب اللي هو + +419 +00:29:59,700 --> 00:30:05,200 +السائل اللي موجود بس يسحب من طبيببيعرف كيف يسحبوا + +420 +00:30:05,200 --> 00:30:08,860 +هذا رقم واحد، رقم اتنين بيعطينا antibiotic قبل ما + +421 +00:30:08,860 --> 00:30:13,420 +يسحبوا، عشان لو بكتيريا تهت و راحت على الدم ال + +422 +00:30:13,420 --> 00:30:16,960 +antibiotic ايه، يقتلها، فال .. ال .. ال systemic + +423 +00:30:16,960 --> 00:30:19,680 +disease غالبا في الـ Staphylococcus aureus بتنشأ + +424 +00:30:19,680 --> 00:30:24,500 +من فوكي، من مكان محدد، زي جرح أو حبة أو الاخر، + +425 +00:30:24,500 --> 00:30:29,310 +osteomyelitis، اليلتهابالعضم والعضلات نيومونيا + +426 +00:30:29,310 --> 00:30:32,990 +اللى هى التهاب الرئى وبنسمى الستافيلو كوكاس + +427 +00:30:32,990 --> 00:30:37,550 +نيومونيا باسم خاص بنسميها استافيلو كوكل نيومونيا + +428 +00:30:37,550 --> 00:30:42,170 +عشان نميزها عن باقي أنواع النيومونيا هذه ال + +429 +00:30:42,170 --> 00:30:48,430 +impetigo اللى حكينا عنهاهى عبارة عن skin infection + +430 +00:30:48,430 --> 00:30:52,790 +طبعا هدى .. هدى ألطف صورة ممكن نلاقيها لو دورتوا + +431 +00:30:52,790 --> 00:30:55,330 +على ال .. على ال google على impetigo هتشوفوا صور + +432 +00:30:55,330 --> 00:30:57,430 +كثيرة mosiacally disturbing أنا جيبت صورة يعني + +433 +00:30:57,430 --> 00:31:02,290 +نوعا ما مقبولة طبعا ال .. ال .. ال .. ال .. اللى + +434 +00:31:02,290 --> 00:31:05,070 +عارفينها في ال chin هدى بعد فترة بيصيرلها white + +435 +00:31:05,070 --> 00:31:08,550 +crustaceanيعني او .. او golden crustacean حتى بكل + +436 +00:31:08,550 --> 00:31:11,870 +لونها ذهبي اللي هي القشرة اللي .. او القشور وزي ما + +437 +00:31:11,870 --> 00:31:15,290 +حكيتلكوا very contagious والسلالة اللي بتسيلف هذا + +438 +00:31:15,290 --> 00:31:18,850 +النوع غالبا بتكون antimicrobial resistance يعني + +439 +00:31:18,850 --> 00:31:22,090 +انها مقاومة antimicrobial يعني ذلك بيلاقي ان + +440 +00:31:22,090 --> 00:31:26,030 +البنسالين اللي كان مناسب للدمام للخلالات غير مناسب + +441 +00:31:26,030 --> 00:31:30,190 +لعلاج اللي هو ال MBT وهذا مؤشر بيأكد الكلام اللي + +442 +00:31:30,190 --> 00:31:34,330 +حكيناه انه بيتكلم عن سلالات مختلفة صحيح اسم واحد + +443 +00:31:34,330 --> 00:31:38,440 +لكن سلالات مختلفةهذه الصفحة دي خلصناها تقريبا، + +444 +00:31:38,440 --> 00:31:41,280 +هتكلمنا عن ال food poisoning وميزنا بين كلمة food + +445 +00:31:41,280 --> 00:31:44,840 +poisoning و food infection ال poisoning إن ال + +446 +00:31:44,840 --> 00:31:49,520 +toxin was formed in the food before eating it + +447 +00:31:49,520 --> 00:31:54,360 +بينما ال food infection هو acted as a carrier for + +448 +00:31:54,360 --> 00:31:58,720 +the infectious agent usually food is contaminated + +449 +00:31:58,720 --> 00:32:01,640 +from humans و بصير له growth و بعدين بيصير فيه انه + +450 +00:32:01,640 --> 00:32:02,920 +enterotoxin + +451 +00:32:05,350 --> 00:32:10,190 +بتكونوا تخيلوا موضوع .. أما موضوع food .. + +452 +00:32:10,190 --> 00:32:14,490 +poisoning و food infection هيحتل معانا course كامل + +453 +00:32:14,490 --> 00:32:15,530 +سنة الجاية + +454 +00:32:21,620 --> 00:32:24,140 +الـ Food Microbiology .. الـ Food Microbiology + +455 +00:32:24,140 --> 00:32:27,100 +صرعنا course بالخطط .. بخطط التحقيق الطبيب والتالي + +456 +00:32:27,100 --> 00:32:31,180 +بنعرف إنه إله أهمية كبرى و خلينا ناخد ال medical + +457 +00:32:31,180 --> 00:32:35,920 +microbiology منطلق لفهم أعمق يعني بمعنى عخر التخيل + +458 +00:32:35,920 --> 00:32:40,400 +اللي أنا ذكرته في صناع غذاء محدد اللي هو المفتول + +459 +00:32:40,400 --> 00:32:45,320 +من .. من خطواته من ألف إلى الياء تمام؟ ممكن نطبق + +460 +00:32:45,320 --> 00:32:48,200 +هذه المفهومة على أي غذاء احنا بنصنعه و نشوف وين + +461 +00:32:48,200 --> 00:32:55,160 +ممكن ي .. يصير تلوث من ..لـ human لأن أنظمة الجودة + +462 +00:32:55,160 --> 00:32:59,100 +بتصنيع الغذاء اليوم بتتنش على إن المنتج النهائي + +463 +00:32:59,100 --> 00:33:04,320 +يكون جيد و بس لازم من ال raw material من المواد + +464 +00:33:04,320 --> 00:33:09,100 +الخالة إلى المنتج يكون في عملية مراقبة لخط الإنتاج + +465 +00:33:09,100 --> 00:33:11,720 +و أنا بقصد خط الإنتاج مش ضروري مصنع ممكن في البيت + +466 +00:33:11,720 --> 00:33:16,290 +كمانما هو في الأخر خطوات ولا لأ، أي تصنيع، أي حاجة + +467 +00:33:16,290 --> 00:33:18,890 +بنعملها هي عبارة عن خطوات، تجاربنا في المختبر + +468 +00:33:18,890 --> 00:33:22,470 +عبارة عن خطوات، فمن أول خطوة إلى آخر خطوة لازم + +469 +00:33:22,470 --> 00:33:25,110 +يكون في أندي مقدرة على أنه أسيطر على العملية بحيث + +470 +00:33:25,110 --> 00:33:28,970 +أنه وين في احتمالية contamination أعمل إجراءات + +471 +00:33:28,970 --> 00:33:30,670 +تمنع اللي هو ال contamination + +472 +00:33:35,890 --> 00:33:38,830 +ال baby with scalded skin syndrome اللي هنقول عنهم + +473 +00:33:38,830 --> 00:33:44,090 +exfoliating اللي هو بيعمل epidermal lysis ل ال + +474 +00:33:44,090 --> 00:33:48,270 +skin وبالتالي إذا بتلاحظوا الصورة واضح جدا أنه في + +475 +00:33:48,270 --> 00:33:55,390 +عندنا لونين لون و لون وفي عندنا حواف لجلد بدأي + +476 +00:33:55,390 --> 00:33:59,630 +برضه كمان بيكون continuous shedding ل ال skin + +477 +00:33:59,630 --> 00:34:03,630 +laboratory + +478 +00:34:04,370 --> 00:34:09,030 +هناخدوا بالكوا في ال medical microbiology هعمل من + +479 +00:34:09,030 --> 00:34:12,510 +الان وصاعدا هعمل لوحة واحدة لل laboratory + +480 +00:34:12,510 --> 00:34:17,810 +diagnosis عشان ال diagnostic microbiology هو أصلا + +481 +00:34:17,810 --> 00:34:21,050 +الشغل اللي فيها ال diagnosis وبتاع مش هنلقى ال + +482 +00:34:21,050 --> 00:34:25,650 +course التاني هنا لكن معنيين أنه ناخد فكرة عامة عن + +483 +00:34:25,650 --> 00:34:28,190 +ال laboratory diagnosis أي تفاصيل بعد اللوحة هذه + +484 +00:34:28,190 --> 00:34:33,300 +هي للشرح فقط لغايةيعني ليس مطالبين إلا باللوحة + +485 +00:34:33,300 --> 00:34:35,540 +الرئيسية اللي مكتوب عليها laboratory diagnosis لان + +486 +00:34:35,540 --> 00:34:43,360 +تزيد عن صفحة لان تزيد عن لوحة واحد Staphylococcus + +487 +00:34:43,360 --> 00:34:46,380 +aureus إيش سمناها؟ احنا جاب شوية من ناحية حرية ال + +488 +00:34:46,380 --> 00:34:52,360 +oxygen في عقل تاتر عنوغرخص نعمل incubation هوائي + +489 +00:34:52,360 --> 00:34:57,000 +ولا لهوائي؟ هوائي اللي لا هوائيمع الحاجة دي بيزيل + +490 +00:34:57,000 --> 00:35:01,720 +الهواء، اذا توفير بيئة لا هوائية هو بيكلفنا فلوس + +491 +00:35:01,720 --> 00:35:04,340 +لإن احنا بدنا نضطر إنه ننزع الهواء اللي هو أصلا + +492 +00:35:04,340 --> 00:35:10,460 +موجود بشكل natural ممكن .. قولنالكوا العينة اللي + +493 +00:35:10,460 --> 00:35:13,340 +فيها صديد لو عملناها gram stain مباشرة نشوف + +494 +00:35:13,340 --> 00:35:17,720 +البكتيريا، لكن هل يمكن من خلالها أنه نفتي أن + +495 +00:35:17,720 --> 00:35:20,980 +البكتيريا أستفيد من cocas aureas؟ لأ، بتساعدنا + +496 +00:35:21,910 --> 00:35:26,470 +زراعتنا على مين نزرع؟ على ال blood agar كتير بتنمو + +497 +00:35:26,470 --> 00:35:29,850 +بشكل جيد على ال blood agar وإنها مميزات على ال + +498 +00:35:29,850 --> 00:35:33,830 +blood agar في المحاضرة الماضية وانا قاعد بشرح على + +499 +00:35:33,830 --> 00:35:38,370 +أهمية شكل المستعمرة حاولت أوصف المستعمرة تبعت ال + +500 +00:35:38,370 --> 00:35:42,190 +Staphylococcus aureus على ال blood agar فقول large + +501 +00:35:42,190 --> 00:35:45,850 +two to four millimeter rounded golden yellow + +502 +00:35:45,850 --> 00:35:52,240 +pigmented colonies on blood agarواليوم المضيف انها + +503 +00:35:52,240 --> 00:35:55,720 +hemolytic فاكريني ال hemolycine؟ يعني بتاع ما ال + +504 +00:35:55,720 --> 00:35:59,520 +beta hemolysis الان خليني اتكلم عن ال beta + +505 +00:35:59,520 --> 00:36:02,720 +hemolysis و ال alpha hemolysis و ال no hemolysis + +506 +00:36:02,720 --> 00:36:06,640 +راجزوا معايا في الكلامات اللي بتحكيها من مولودة + +507 +00:36:06,640 --> 00:36:13,100 +على هذا السبورة ال blood agar ال blood agar إذا + +508 +00:36:13,100 --> 00:36:17,920 +زولت عليه بكتيريا فهناك ثلاث احتمالات تتعلق بال + +509 +00:36:17,920 --> 00:36:23,900 +hemolysisالاحتمال الاول ان يكون هناك تحلل كامل + +510 +00:36:23,900 --> 00:36:30,920 +للدم حول المستعمرة ويطلق على هذا النوع من التحلل + +511 +00:36:30,920 --> 00:36:35,660 +Beta Hemolysis Complete Hemolysis Beta Hemolysis + +512 +00:36:35,660 --> 00:36:39,360 +كيف بنعرف انه Beta Hemolysis لون ال blood agar + +513 +00:36:39,360 --> 00:36:42,960 +بيكون أحمر بيصير colorless يعني المستعمرة بيصير + +514 +00:36:42,960 --> 00:36:48,920 +حولها منطقة خالية من اللونخلّي من اللون للمستعمرة + +515 +00:36:48,920 --> 00:36:54,740 +هذا بيتا هموليسيس الاحتمال التاني incomplete + +516 +00:36:54,740 --> 00:37:00,860 +hemolysis او partial طبعا مصطلحها كويس partial او + +517 +00:37:00,860 --> 00:37:06,800 +incomplete hemolysis كيف نصدر عليه بصير اللون الدم + +518 +00:37:06,800 --> 00:37:11,460 +بدل ما هو أحمر أخضر نعم greenish لأنه incomplete + +519 +00:37:11,460 --> 00:37:12,180 +hemolysis + +520 +00:37:15,870 --> 00:37:20,990 +وبنسميها Alpha الاحتمال + +521 +00:37:20,990 --> 00:37:26,410 +التالت ان لا يتغير شيء يعني لا هموليسيس لكمبيوتر + +522 +00:37:26,410 --> 00:37:29,710 +لإن كمبيوتر بنسميها No Hemolysis ويلها اسم عشان ما + +523 +00:37:29,710 --> 00:37:33,970 +تزعلش Gamma Hemolysis يبقى عندنا بنقول Gamma + +524 +00:37:33,970 --> 00:37:38,630 +Hemolysis جاهزة ترضية جاهزة ترضية للبكتيرى اللي + +525 +00:37:38,630 --> 00:37:43,310 +بتمالش Hemolysis إذا Beta Alpha Gamma + +526 +00:37:49,370 --> 00:37:52,510 +ماعشها هذه المعلومات الهامش، هستفيد منها لاحقا ان + +527 +00:37:52,510 --> 00:37:56,070 +شاء الله لما اتكلم عن ال laboratory diagnosis ال + +528 +00:37:56,070 --> 00:37:59,810 +differential test منطول كواجوليز و DNA هاي + +529 +00:37:59,810 --> 00:38:02,350 +الفيصولات اللي هتستخدمها في التفريق بينها وبين + +530 +00:38:02,350 --> 00:38:07,350 +السللات الأخرى او اللي اتقفن ال spaces الأخرى من + +531 +00:38:07,350 --> 00:38:11,330 +الstaphylococcus بينما إذا بدى ميزها عن + +532 +00:38:11,330 --> 00:38:15,250 +الstriptococci إيش بدي كتاليز + +533 +00:38:26,690 --> 00:38:31,470 +الفحص في مكان المناسب لا يلوز ان اول فحص في غير + +534 +00:38:31,470 --> 00:38:34,730 +مكان المناسب يعني انت اتعلمت في المختبر عشرين فحص + +535 +00:38:34,730 --> 00:38:40,950 +او تلاتين فحص مش تجيبهم كلاتهم اختيارهم مبني على + +536 +00:38:40,950 --> 00:38:45,810 +مينعلى معلومات تتبع معلومات، أعطيكم مثال، عزرت + +537 +00:38:45,810 --> 00:38:49,270 +بكتيريا أنا على طبق اسمه بلداجات، عملتلها gram + +538 +00:38:49,270 --> 00:38:54,410 +stain، طلعت gram-bosticoxide، what is next؟ + +539 +00:38:54,410 --> 00:38:59,970 +كتاليز، طلعت negative، كتاليز negative، يعني ايش + +540 +00:38:59,970 --> 00:39:00,050 +ال + +541 +00:39:13,400 --> 00:39:18,640 +اختياري للفحوصات كان معتمد على مين؟ على تسلسل .. + +542 +00:39:18,640 --> 00:39:24,240 +تسلسل .. أحداث معينة مابجيش انا اقول ال + +543 +00:39:24,240 --> 00:39:28,700 +Staphylococcus aureus جلوكوز فيرمنتر و ال E.coli + +544 +00:39:28,700 --> 00:39:30,580 +جلوكوز فيرمنتر كيف ده ميزوا من بعض؟ + +545 +00:39:34,680 --> 00:39:37,520 +طب واحدة كوكساية واحدة بسالاية يعني هم أساسا + +546 +00:39:37,520 --> 00:39:40,900 +المختلفين ليش انا .. انا .. ليش أخطأت و جيبت صفة + +547 +00:39:40,900 --> 00:39:43,980 +تتشارك مع بعض فيهم عشان أميزهم مع بعض أساسا هم + +548 +00:39:43,980 --> 00:39:48,520 +المميزين الأحسان الأبيض و الأحسان الأسود تمام؟ هم + +549 +00:39:48,520 --> 00:39:51,700 +أصلا مميزين وبالتالي مافيش داعي أن أدوى على صفات + +550 +00:39:51,700 --> 00:39:56,600 +أخرى أعقدهم الموضوع قد تتشارك الميكروبات في صفات + +551 +00:39:56,600 --> 00:39:59,900 +معينة لكن من الأساس أنا أميزهم بال Gram Stain Gram + +552 +00:39:59,900 --> 00:40:03,470 +Positive و Gram Negative بسالاية أو كوكسايةإذن + +553 +00:40:03,470 --> 00:40:07,090 +عشان انا اقدر أقول ان البكتيريا هي استفيد ل كوكاين + +554 +00:40:07,090 --> 00:40:10,290 +لازم يكون عندى معلومتين إنها gram-bostev-coccyte + +555 +00:40:10,290 --> 00:40:17,310 +إذا اعتبرتوها معلومتين لحالهم فتلت معلومات gram + +556 +00:40:17,310 --> 00:40:19,150 +-bostev-coccyte + +557 +00:40:21,680 --> 00:40:26,160 +التلت معلومات لحد الآن لحد اللحظة هذه بيشيروا ان + +558 +00:40:26,160 --> 00:40:29,420 +البكتيريا هي Staphylococcus مين من ال + +559 +00:40:29,420 --> 00:40:33,880 +Staphylococci؟ المانيتول و ال coagulase و ال DNA's + +560 +00:40:33,880 --> 00:40:36,240 +اللي مردها الصبورة ده اللي هم اللي بيميزوه منهم + +561 +00:40:36,240 --> 00:40:44,660 +الفاش typing فاكرين الفاش؟ البكتيروفاج البكتيروفاج + +562 +00:40:44,660 --> 00:40:49,140 +كان فيه منه نوع سمناه lightic phage اللي بيخش على + +563 +00:40:49,140 --> 00:40:53,880 +البكتيريا و بيحللهااللوحة اللي قدامكوا هذه الصورة + +564 +00:40:53,880 --> 00:40:58,560 +طبق هو عبارة عن طبقة من البكتيريا نامية وفي مناطق + +565 +00:40:58,560 --> 00:41:04,860 +فارغة من النمو black blacks اللي هي اللي هي الأثر + +566 +00:41:04,860 --> 00:41:08,460 +اللي بتركه البكتيريا فارج من تحلل الخلازة منها + +567 +00:41:08,460 --> 00:41:12,520 +blacks وإذا بتذكروا كنا نعدل الفيروسات عن طريق ال + +568 +00:41:12,520 --> 00:41:17,330 +black بنقول black warming unitالآن لو أنا عندي + +569 +00:41:17,330 --> 00:41:21,290 +بكتيريا مجهولة اسمها staphylococcus تمام؟ و بدى + +570 +00:41:21,290 --> 00:41:25,650 +أعرفها aureus ولا epidermis و أنا عندي في التلاجة + +571 +00:41:25,650 --> 00:41:28,730 +bacteriophage لل aureus و واحد لل epidermis و واحد + +572 +00:41:28,730 --> 00:41:32,830 +لل saprophytic و البكتيرى اللى قدامى هذه بدى أعرف + +573 +00:41:32,830 --> 00:41:36,610 +إنها aureus ولا لا حاطط عليها bacteriophage زى ما + +574 +00:41:36,610 --> 00:41:41,090 +تشوفين في الصورة تمام؟ حاطط عليها bacteriophage + +575 +00:41:41,400 --> 00:41:44,200 +تانى يوم البكتيريا نمت لك اللى جت فيها ثقوب معناه + +576 +00:41:44,200 --> 00:41:48,080 +الحاكى اشهر ان البكتيريا فاج specific لها معناه + +577 +00:41:48,080 --> 00:41:52,460 +الحاكى ان البكتيريا فاج specific هى مجهولة هو + +578 +00:41:52,460 --> 00:41:55,920 +معلوم الان الاتفاعل كمان بتتكلم عن التفاعل + +579 +00:41:55,920 --> 00:41:59,700 +specific الاتفاعل المجهول مع المعلوم أصبح المجهول + +580 +00:41:59,700 --> 00:42:04,080 +علمه و تانى من جثصنا يبجي عملنا بالفعل معارفة او + +581 +00:42:04,080 --> 00:42:08,680 +تعرف على البكتيريا من خلال اللى هو البكتيريوم فيه + +582 +00:42:08,680 --> 00:42:14,920 +هنا ال antibiotic sensitivityTesting لما أنا بشرح + +583 +00:42:14,920 --> 00:42:20,560 +محاضرات عامة عن ال antimicrobial resistance أول + +584 +00:42:20,560 --> 00:42:26,700 +قصة ببدأ فيها كنموذج عن ال resistance بتكلم عن ال + +585 +00:42:26,700 --> 00:42:31,060 +Staphylococcus aureus في أربعينيات القرن الماضي + +586 +00:42:31,060 --> 00:42:35,580 +كانت ال Staphylococcus aureus وزي ما ذكرتلكوا ال + +587 +00:42:35,580 --> 00:42:38,020 +wound infection و ال birth infection و الدنامل و + +588 +00:42:38,020 --> 00:42:42,680 +الخرجات يعني منتشرة في العالم بشكل كبير جداإيجي + +589 +00:42:42,680 --> 00:42:48,660 +البنسالين وكان ال magic drug فاش + +590 +00:42:48,660 --> 00:42:50,880 +يستفيد الكوكا ال infection كانت تصمد قدام + +591 +00:42:50,880 --> 00:42:57,020 +البنسالين بعد 15 سنة بدأت تظهر نتائج سيئة للغاية + +592 +00:42:57,020 --> 00:43:00,320 +للبنسالين البنسالين بطل صناعة ال injection و + +593 +00:43:00,320 --> 00:43:04,600 +double injection على الفاضي resistance اكتشفنا + +594 +00:43:04,600 --> 00:43:08,060 +عملنا modification للبنسالين عملنا وكسلين وامبسلين + +595 +00:43:08,060 --> 00:43:12,500 +هدولا semisyntheticحالينا فيهم بعض الإشكاليات بعد + +596 +00:43:12,500 --> 00:43:16,120 +سنتين تلاتة بس بدأ يكون Resistance كوّننا Oxycelin + +597 +00:43:16,120 --> 00:43:18,400 +و Methicillin كوّنت Resistance صار اسمها + +598 +00:43:18,400 --> 00:43:21,140 +Methicillin Resistance Staphylococcus Aureus أو + +599 +00:43:21,140 --> 00:43:24,920 +MRSA مشهور جدا هذه البكتيريا إجرينا Vancomycin + +600 +00:43:24,920 --> 00:43:30,440 +وقلنا هذا الحل Vancomycin هو الحل فبعد أدت سنوات + +601 +00:43:30,440 --> 00:43:34,120 +لجينا visa visa Vancomycin Intermediate + +602 +00:43:34,120 --> 00:43:36,320 +Staphylococcus Aureus بعد كام من سنة لجينا + +603 +00:43:36,320 --> 00:43:40,780 +Vancomycin Resistance Staphylococcus Aureusيبجي + +604 +00:43:40,780 --> 00:43:44,880 +البكتيريا دي شمالها يعني إيش أنتوا بتعملوا + +605 +00:43:44,880 --> 00:43:48,320 +antibiotics تمام؟ هي عند المقضرة عينها تكون + +606 +00:43:48,320 --> 00:43:51,920 +resistance وبالتالي أنا صرت أخاف كتير من هذه + +607 +00:43:51,920 --> 00:43:55,060 +البكتيريا باعتبرها من الميكروبات الخطيرة جدا وكتير + +608 +00:43:55,060 --> 00:43:58,540 +من الدراسات اللي أجرينها الصحيح أجرينها على الـ + +609 +00:43:58,540 --> 00:44:01,920 +Staphylococcus aureus لجينا على سبيل المثال عند + +610 +00:44:01,920 --> 00:44:05,160 +الأخوة و الأخوات اللي عاملين في مستشفى الشفاء لما + +611 +00:44:05,160 --> 00:44:09,260 +خدنا الموبايلات بتاعتهم لجينا نسبة منهم عندهم MRSC + +612 +00:44:09,260 --> 00:44:12,060 +على الموبايلاتيعني بيروحوها على الدار معاهم + +613 +00:44:12,060 --> 00:44:16,240 +وبعطوها للولاد وولادهم بيعطوها للناس تانين فبتصيب + +614 +00:44:16,240 --> 00:44:20,100 +المستشفى كائن هى مجتمع فاكرين لما قسمنا احنا كنا + +615 +00:44:20,100 --> 00:44:23,580 +hospital infection و community acquired infection + +616 +00:44:23,580 --> 00:44:28,280 +اللي احنا بتكلم على انه مجتمع شبيه بمين ببيت طبعا + +617 +00:44:28,280 --> 00:44:31,660 +ماوصلنا احنا للمستشفى لكن على الأقل المستشفى واضح + +618 +00:44:31,660 --> 00:44:35,100 +انه بيعمل دخل لمايكروبات تبعته في ال community + +619 +00:44:35,100 --> 00:44:39,060 +تبعته ال antibiotic sensitivity أنا اختبت فيها + +620 +00:44:39,060 --> 00:44:43,750 +المحاضرة اللى فاتتلما اتكلمت عن ال diagnosis او ال + +621 +00:44:43,750 --> 00:44:46,230 +detection و prevention او prophylactis and + +622 +00:44:46,230 --> 00:44:49,630 +treatment قلتلكوا انا احنا مسئولييتنا في المختبر + +623 +00:44:49,630 --> 00:44:53,210 +نعزل الميكروب اللي هو ال pathogen من العينة و + +624 +00:44:53,210 --> 00:44:57,550 +نتعرف عليه و نعمله antimicrobial sensitivity او + +625 +00:44:57,550 --> 00:45:01,010 +susceptibility نفس المعنى testing اختيارنا لل + +626 +00:45:01,010 --> 00:45:05,730 +antibiotic اللي هنستخدمهم منطب اسم البكتيريا منطب + +627 +00:45:05,730 --> 00:45:10,170 +الأمر تبع المريض يعني اعطيكم مثال على الأمرتعرف + +628 +00:45:10,170 --> 00:45:14,710 +تترا ساكلين؟ تترا ساكلين، antibiotic مشهور جدا و + +629 +00:45:14,710 --> 00:45:18,910 +very effective أو على الأقل كان very effective لكن + +630 +00:45:18,910 --> 00:45:25,450 +لا يلوز إعطاءه لا للطفل ولا للمرأة الحامل ولا + +631 +00:45:25,450 --> 00:45:30,170 +للمرأة المرضى، فالمرضى احنا خايفين على مش الأم + +632 +00:45:30,170 --> 00:45:36,390 +خايفين على الجنين و في الحامل خايفين على التنين و + +633 +00:45:36,390 --> 00:45:40,830 +الطفل خايفين على الطفل طبعايعني مابنقدرش نعطيه، + +634 +00:45:40,830 --> 00:45:42,790 +فانا اقول يا عزالة انا استفيد الكوكس اورس من + +635 +00:45:42,790 --> 00:45:46,090 +الطفل، مابحطلهوش قُرص مين تترا ساكين على سمين + +636 +00:45:46,090 --> 00:45:49,710 +مثال، فاختيارنا ال antibiotic مرتبط لمين قلناه؟ مش + +637 +00:45:49,710 --> 00:45:53,590 +.. هذا واحد، ذاري أقول، بين واحد بكتيريا بالعمر + +638 +00:45:53,590 --> 00:45:56,750 +male ولا female، physiological state، هل ال + +639 +00:45:56,750 --> 00:45:59,930 +pregnancy .. he safe لل pregnancy و he not safe لل + +640 +00:45:59,930 --> 00:46:04,710 +pregnancy؟المكان تبع الإصابة هل من ال prostate ولا + +641 +00:46:04,710 --> 00:46:08,150 +من الدم بفرق لإنه مش كل ال antibiotic بتصل ل ال + +642 +00:46:08,150 --> 00:46:11,870 +prostate تمام فاختيارنا ال antibiotic مرتبط بمين + +643 +00:46:11,870 --> 00:46:16,030 +بمجموعة من العوامل طبعا هتعلمهم في ال .. ال .. ال + +644 +00:46:16,030 --> 00:46:18,090 +.. العامل القادم أو في الفصل القادم ان شاء الله في + +645 +00:46:18,090 --> 00:46:21,510 +ال diagnostic لكن في 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{"start": 1115.91, "end": 1116.41, "word": " معقد،", "probability": 0.8876953125}, {"start": 1116.41, "end": 1116.67, "word": " بطل", "probability": 0.87451171875}, {"start": 1116.67, "end": 1117.03, "word": " جلسل", "probability": 0.7132568359375}, {"start": 1117.03, "end": 1117.41, "word": " أكبر", "probability": 0.8893229166666666}, {"start": 1117.41, "end": 1118.89, "word": " فصابغنا", "probability": 0.75771484375}, {"start": 1118.89, "end": 1119.77, "word": " و", "probability": 0.7333984375}, {"start": 1119.77, "end": 1120.07, "word": " بعدين", "probability": 0.760986328125}, {"start": 1120.07, "end": 1120.63, "word": " ثبتنا", "probability": 0.9691162109375}, {"start": 1120.63, "end": 1121.07, "word": " اللون", "probability": 0.927734375}, {"start": 1121.07, "end": 1121.85, "word": " بالأيودين", "probability": 0.83037109375}], "temperature": 1.0}, {"id": 40, "seek": 115171, "start": 1121.93, "end": 1151.71, "text": "الأيودين الشمالي هنا مثبت لللون خطوة التالتة الحاسمة ضفنا مادة decolorizer مادة مزيل لللون وهي عبارة عن إيثانول إيثانول بتعرفوه ال إيثانول بينجح بإزالة اللون من ال gram negative ولا ينجح بإزالة اللون من مين؟ من ال gram positive أحنا ماكنناش اللون الأصلي للبكتيريا شفافة يبقى هتش صار فيها اللون هنا رجعت شفافة", "tokens": [6027, 10721, 1829, 23328, 9957, 25124, 2304, 6027, 1829, 34105, 50113, 3555, 2655, 24976, 1211, 11536, 16490, 9566, 2407, 3660, 16712, 6027, 2655, 3660, 21542, 32277, 46007, 48812, 5172, 8315, 3714, 18513, 3660, 979, 401, 284, 6545, 3714, 18513, 3660, 3714, 11622, 26895, 24976, 1211, 11536, 37037, 1829, 6225, 3555, 9640, 3660, 18871, 11933, 1829, 12984, 7649, 12610, 11933, 1829, 12984, 7649, 12610, 39894, 3615, 28480, 2407, 3224, 2423, 11933, 1829, 12984, 7649, 12610, 4724, 9957, 7435, 5016, 4724, 28814, 11622, 6027, 3660, 13672, 11536, 9154, 2423, 21353, 3671, 49429, 7251, 1863, 7435, 5016, 4724, 28814, 11622, 6027, 3660, 13672, 11536, 9154, 3714, 9957, 22807, 9154, 2423, 21353, 3353, 5551, 5016, 8315, 19446, 19452, 8315, 8592, 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{"start": 1501.07, "end": 1501.63, "word": " جدا", "probability": 0.79541015625}, {"start": 1501.63, "end": 1502.07, "word": " في", "probability": 0.953125}, {"start": 1502.07, "end": 1502.55, "word": " ال", "probability": 0.83447265625}, {"start": 1502.55, "end": 1504.33, "word": " gram", "probability": 0.7802734375}, {"start": 1504.33, "end": 1504.77, "word": "-bosted", "probability": 0.9490966796875}, {"start": 1504.77, "end": 1505.37, "word": " بكتيريا", "probability": 0.9482421875}, {"start": 1505.37, "end": 1505.81, "word": " حارس", "probability": 0.755126953125}, {"start": 1505.81, "end": 1506.03, "word": " انها", "probability": 0.55267333984375}, {"start": 1506.03, "end": 1506.89, "word": " تايكويكاسيد", "probability": 0.6001325334821429}], "temperature": 1.0}, {"id": 54, "seek": 153684, "start": 1507.44, "end": 1536.84, "text": "والـ Tychoic Acid زي ما مقوله في اللوحة ماخد أو جاي من مكانين واحد جاي من ال cell membrane من تحته، شايفينه؟ اللي دول تبعته؟ واحد جاي من ال cell wall اللي جاي من ال cell wall بنطلق عليه هكذا، Tychoic Acid التاني اللي جاي من ال cell membrane بنطلق عليه Lipo-Tychoic Acid Lipo لإنه جاى من ال lipids تمام، Lipo-Tychoic Acid", "tokens": [2407, 6027, 39184, 5569, 5738, 299, 5097, 327, 30767, 1829, 19446, 3714, 39648, 3224, 8978, 13672, 2407, 5016, 3660, 3714, 47283, 3215, 34051, 10874, 47302, 9154, 3714, 41361, 9957, 36764, 24401, 10874, 47302, 9154, 2423, 2815, 19651, 9154, 6055, 33753, 3224, 12399, 13412, 995, 33911, 9957, 3224, 22807, 13672, 1829, 11778, 12610, 6055, 3555, 34268, 3224, 22807, 36764, 24401, 10874, 47302, 9154, 2423, 2815, 2929, 13672, 1829, 10874, 47302, 9154, 2423, 2815, 2929, 44945, 9566, 1211, 4587, 47356, 8032, 4117, 15730, 12399, 5569, 5738, 299, 5097, 327, 16712, 7649, 1829, 13672, 1829, 10874, 47302, 9154, 2423, 2815, 19651, 44945, 9566, 1211, 4587, 47356, 27475, 78, 12, 22464, 5738, 299, 5097, 327, 27475, 78, 5296, 28814, 1863, 3224, 10874, 995, 7578, 9154, 2423, 8280, 3742, 6055, 15042, 2304, 12399, 27475, 78, 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"text": "يعني مثلا صلب زي الورقة يعني اللي بنمزعه و لا زي الجلد تبعنا طري و لا ايش؟ كيف القوام تبعه؟ سننبراني اوضحلكوا كيف القوام تبعه، دي بصحن زيت زيتون، بحط أصبعك في الصحن و امسكيه هك، ايش بيصير في زيت زيتون؟ بنكسبه نصين، تمام؟ و بعد ما احنا أصبعنا يمر، ايش بيعمل؟ ترجع تاني", "tokens": [40228, 22653, 50113, 15040, 20328, 46152, 30767, 1829, 2423, 13063, 28671, 37495, 22653, 13672, 1829, 44945, 2304, 11622, 3615, 3224, 4032, 20193, 30767, 1829, 25724, 1211, 3215, 6055, 3555, 3615, 8315, 23032, 16572, 4032, 20193, 1975, 1829, 8592, 22807, 9122, 33911, 25062, 2407, 10943, 6055, 3555, 3615, 3224, 22807, 8608, 1863, 1863, 26890, 7649, 1829, 1975, 2407, 11242, 5016, 23275, 14407, 9122, 33911, 25062, 2407, 10943, 6055, 3555, 3615, 3224, 12399, 11778, 1829, 4724, 9381, 5016, 1863, 30767, 36081, 30767, 36081, 11536, 12399, 4724, 5016, 9566, 5551, 9381, 3555, 3615, 4117, 8978, 31767, 5016, 1863, 4032, 1975, 2304, 3794, 4117, 1829, 3224, 8032, 4117, 12399, 1975, 1829, 8592, 4724, 1829, 9381, 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جديد هنبدأ فيه إن شاء الله + +2 +00:00:23,840 --> 00:00:28,120 +الله اليوم اللي هو الـ gram negative بس اللهم إني من + +3 +00:00:28,120 --> 00:00:30,700 +أول ما بتكلم عن الـ gram stain قولتلكوا الـ gram + +4 +00:00:30,700 --> 00:00:35,900 +stain بيقسم البكتيريا إلى أربع مجموعات إما gram + +5 +00:00:35,900 --> 00:00:40,880 +negative أو gram positive وكل من هما باس الله أو + +6 +00:00:40,880 --> 00:00:44,100 +كوكساي وأعتقد أنه في المحاضرات السابقة تعرّفنا + +7 +00:00:44,100 --> 00:00:48,960 +لنوعين احنا اللي هم الـ gram negative كوكساي متمثلة + +8 +00:00:48,960 --> 00:00:52,900 +في النيسيريا وgram positive كوكساي متمثلة في + +9 +00:00:52,900 --> 00:00:57,080 +الاستافيلوكوكس والاسترپتوكوكس والانتروكوكس لكن + +10 +00:00:57,080 --> 00:01:00,720 +من أول المحاضرات وأنا حضرتها وأنت تلاقي أضخم + +11 +00:01:00,720 --> 00:01:04,540 +مجموعة على الإطلاق هي الـ gram negative بس الـ lie + +12 +00:01:04,540 --> 00:01:08,320 +اللي احنا بتكلمنا اليوم فتوقع أنه برضه يعني يكون + +13 +00:01:08,320 --> 00:01:13,820 +في كم كبير من المعلومات متعلقة بهذه المجموعة عندنا + +14 +00:01:13,820 --> 00:01:17,320 +كمان مرة gram stain gram negative باللون الزهري والأحمر وgram positive الـ gram negative زي ما + +15 +00:01:17,320 --> 00:01:20,860 +انتوا شايفين من قسم الـ + +16 +00:01:20,860 --> 00:01:22,840 +طبعا احنا هنتكلم على الـ gram negative بس الله نطلع + +17 +00:01:27,750 --> 00:01:30,990 +طبعا احنا هنتكلم على الـ gram negative بس الله نطلع + +18 +00:01:30,990 --> 00:01:35,230 +على الـ diagram برضه تاني هذا كتير مهم إذا بتلاحظوا + +19 +00:01:35,230 --> 00:01:40,350 +أنه الـ .. الـ diagram مقسم البكتيريا إلى قسمين اللي + +20 +00:01:40,350 --> 00:01:44,170 +هم الـ oxidized positive بالـ verbal color والـ + +21 +00:01:44,170 --> 00:01:47,390 +oxidized negative اللي هي colorless سواء كده + +22 +00:01:47,390 --> 00:01:51,370 +positive أو negative برضه مجسمينها جسمين بالاعتماد على + +23 +00:01:51,370 --> 00:01:58,020 +الـ glucose fermentation خديني على اللوحة ناخد + +24 +00:01:58,020 --> 00:02:02,080 +الأسماء الأربعة اللي بيقولوا الأخطر، ناخد أي واحدة + +25 +00:02:02,080 --> 00:02:03,900 +منهم، enterobacterias على سبيل المثال، الـ + +26 +00:02:03,900 --> 00:02:07,740 +enterobacterias هي، نبدأ من تحت ونطلع فوق، هي + +27 +00:02:07,740 --> 00:02:11,620 +positive للـ glucose fermentation، negative + +28 +00:02:11,620 --> 00:02:15,680 +للـ oxidase، ولأن هو الرئيسي gram negative، بس الله، + +29 +00:02:15,680 --> 00:02:20,000 +ناخد Sodomaurus It is negative glucose fermentation + +30 +00:02:20,000 --> 00:02:24,920 +positive for oxidized gram-negative bacilli إذا + +31 +00:02:24,920 --> 00:02:29,520 +استطعنا من خلال تلت صفات تلت صفات الـ gram-negative + +32 +00:02:29,520 --> 00:02:33,580 +bacilli والـ oxidized والـ glucose fermentation أن + +33 +00:02:33,580 --> 00:02:38,340 +نقسم كل gram-negative bacilli إلى أربع مجموعات + +34 +00:02:38,340 --> 00:02:42,510 +مشهورة جدا اللي هي الاسينيتوفاكتات negative + +35 +00:02:42,510 --> 00:02:46,370 +negative الـ enterobacterial negative positive الـ + +36 +00:02:46,370 --> 00:02:49,690 +vibrio positive positive الـ pseudomonas positive + +37 +00:02:49,690 --> 00:02:53,050 +negative أعتقد مش صعبين نحفظهم وضروري نحفظهم + +38 +00:02:53,050 --> 00:03:00,030 +التصنيف هذا بالذات .. التصنيف هذا بالذات كتير مهم + +39 +00:03:00,030 --> 00:03:03,090 +لأنه practical في الآخر بتجيني ground negative بس + +40 +00:03:03,090 --> 00:03:06,750 +الـ like بتعرف عليها أي من الأربع بالفحصين اللي أنا + +41 +00:03:06,750 --> 00:03:08,050 +ذكرتهم بكل سهولة + +42 +00:03:10,720 --> 00:03:16,240 +هنبدأ بالمجموعة الأولى انتروباكتريياسي عشان نقول إن + +43 +00:03:16,240 --> 00:03:19,640 +بكتيريا X هي انتروباكتريياسي لازم تبدأ بخمس صفات + +44 +00:03:19,640 --> 00:03:24,980 +وبنحفظهم الآن في المحاضرة سهلين جدا it is gram + +45 +00:03:24,980 --> 00:03:28,540 +negative بس الله هي واحدة رقم اتنين oxidase + +46 +00:03:28,540 --> 00:03:31,780 +negative هذي مهمة جدا تخيل ما عاملنا الآن على + +47 +00:03:31,780 --> 00:03:35,180 +انتهاء الوقت بكتريياسي oxidase negative تلاتة + +48 +00:03:35,180 --> 00:03:39,440 +جلوكوز في الفرمنتيشن طب هدول التلاتة أنا شفتهم في الـ + +49 +00:03:39,440 --> 00:03:44,840 +diagram اللي جابل صح أضاف لهم كمان صفتين الصفة + +50 +00:03:44,840 --> 00:03:47,500 +الأولى they are all facultative anaerobic they can + +51 +00:03:47,500 --> 00:03:53,430 +grow aerobically and anaerobically رقم خمسة they can + +52 +00:03:53,430 --> 00:03:57,610 +reduce nitrate اللي هو الـ NO3 to nitrite اللي هو + +53 +00:03:57,610 --> 00:04:05,070 +الـ NO2 إذا عندي خمس صفات رئيسية إذا توفرت في أي + +54 +00:04:05,070 --> 00:04:08,170 +ميكروب بطلق عليه لقب enterobacteris + +55 +00:04:10,970 --> 00:04:13,710 +فاكولتاتيف أناروبيك they reduce nitrate to + +56 +00:04:13,710 --> 00:04:17,210 +nitrite, they are all glucose fermenter, they are + +57 +00:04:17,210 --> 00:04:19,970 +all oxidase negative, they are all gram negative + +58 +00:04:19,970 --> 00:04:24,430 +بس اللي لأن هناك صفات أخرى إضافية قد تساعدنا + +59 +00:04:24,430 --> 00:04:28,750 +بالتعرف عليها if motile للـ enterobacterias إذا + +60 +00:04:28,750 --> 00:04:31,270 +كانت متحركة هل معناها الحاجة إن في منها متحرك + +61 +00:04:31,270 --> 00:04:35,310 +ومنها غير متحرك؟ متحرك فإذا كانت متحركة فهي متحركة + +62 +00:04:35,310 --> 00:04:40,120 +بـ peritricus flagella يعني توزيع الفلاجلة على الخلية + +63 +00:04:40,120 --> 00:04:45,120 +اللي بيكون على كل المناطق رقم اتنين they are non + +64 +00:04:45,120 --> 00:04:50,940 +-spore-forming bacteria أنا يعني المعلومة دي مهتمش + +65 +00:04:50,940 --> 00:04:55,500 +فيها كتير ليش؟ لأن أنا أصلا ما بعرفش في كل الـ .. في + +66 +00:04:55,500 --> 00:04:58,380 +كل الـ medical important bacteria إلا اتنين بيكونوا + +67 +00:04:58,380 --> 00:05:02,260 +non-spore-forming وبالتالي ما عدا هم خلاص هم non + +68 +00:05:02,260 --> 00:05:06,890 +-spore-forming هذا الـ diagram عشان يسهل يعني أنا + +69 +00:05:06,890 --> 00:05:10,730 +أحفظه هو نفس المعلومات اللي اللي هو حاسسها يقوله + +70 +00:05:10,730 --> 00:05:14,930 +كمان مرة بنطلع نطلع حافظينهم it is gram negative + +71 +00:05:14,930 --> 00:05:18,390 +بس الله it is oxidase negative because fermented + +72 +00:05:18,390 --> 00:05:22,890 +مش كمان؟ فاكولتاتيف فاكولتاتيف أناروبيك motile non + +73 +00:05:22,890 --> 00:05:26,130 +motile motile peritrichous flagella وقلنا + +74 +00:05:26,130 --> 00:05:29,190 +produces + +75 +00:05:29,190 --> 00:05:32,750 +nitrate to nitrite هذه المعلومة بدنا نتكلم عنها + +76 +00:05:32,750 --> 00:05:38,520 +شوية الـ N O 3 N O 3 بتحول إلى N O 2 + +77 +00:05:38,520 --> 00:05:43,220 +بالانتروباكتريياسي فقط لا غير لكن هناك بعض أنواع من + +78 +00:05:43,220 --> 00:05:47,920 +البكتيريا قد تستطيع تحويل الـ nitrate إلى nitrogen + +79 +00:05:47,920 --> 00:05:55,420 +تتحول من N O 3 إلى N O 2 ومن ثم إلى N 2 بس + +80 +00:05:55,420 --> 00:06:02,900 +الانتروباكتريياسي فقط N O 2 هذه الصفة بتميزنا إن + +81 +00:06:02,900 --> 00:06:06,720 +انتقل الوقت ليها ساعات الأخريات اللي قادرين إن هم + +82 +00:06:06,720 --> 00:06:14,480 +يحولوها إنتوا طيب نجي لجزء الصعب دائما في الـ + +83 +00:06:14,480 --> 00:06:18,120 +micro اللي هو الـ classification قولنا حوالي 29 + +84 +00:06:18,120 --> 00:06:22,440 +جنوس يعني احنا زمان كنا بنجي نقول 14 جنوس أربع عشرة + +85 +00:06:22,440 --> 00:06:27,520 +صاروا ديهم 29 يعني طبعا أي ميكروب نكتشفه وبيمتلك + +86 +00:06:27,520 --> 00:06:31,040 +الخمس صفات اللي احنا حكينا عنهم بنحطهم في تارو + +87 +00:06:31,040 --> 00:06:35,820 +بردر لكن هناك ميكروبات أشهر من ميكروبات بمعنى + +88 +00:06:35,820 --> 00:06:40,460 +أنه أكثر شيوعا أو احنا اتعرفنا عليها منذ فترة + +89 +00:06:40,460 --> 00:06:44,640 +طويلة ودول أصلا بشكل جيد أمراضها واضحة أنهي اللي + +90 +00:06:44,640 --> 00:06:48,400 +اتعاملنا معاها فبالغالب فبالغالب بتكون أشهر من + +91 +00:06:48,400 --> 00:06:54,610 +مجموعات أشهر من مجموعات أخرى على اللوحة مكتوب تلت + +92 +00:06:54,610 --> 00:06:59,130 +ميكروبات أو تلت جينوس باللون الأحمر أو اللون + +93 +00:06:59,130 --> 00:07:04,330 +البني يعني اللون مميزة Shigella Salmonella Yersinia + +94 +00:07:04,330 --> 00:07:09,230 +طب احنا يعني حاطين لهم بلون مختلف نعم لكن قبل ما + +95 +00:07:09,230 --> 00:07:12,170 +نتكلم عن اللون المختلف خلينا نتكلم عن فكرة معينة + +96 +00:07:14,340 --> 00:07:17,280 +سميناها enterobacteriasi، بالك وليه؟ لأنهم + +97 +00:07:17,280 --> 00:07:23,180 +انتيروباتوجينيك، يعني موجودين في القناة الهضمية أو بيعملوا + +98 +00:07:23,180 --> 00:07:26,560 +أمراض في القناة الهضمية، يبقى واحد من اتنين السبب، إما + +99 +00:07:26,560 --> 00:07:29,000 +إنهم موجودين naturally في القناة الهضمية أو إنهم بيعملوا + +100 +00:07:29,000 --> 00:07:32,200 +infection في القناة الهضمية، التلاتة اللي ملونين هدول مش + +101 +00:07:32,200 --> 00:07:36,020 +natural، بيعملوا infection في القناة الهضمية، زي السالمونيلا وال + +102 +00:07:36,020 --> 00:07:37,060 +الشيجيلا وال + +103 +00:07:49,700 --> 00:07:53,940 +الإشريكية، سيتروباكتر، كليبسيلة وانتروباكتر، يا + +104 +00:07:53,940 --> 00:07:59,360 +ريت هدول انحط جنبهم حرف الـ L لكتوز فرمنتر، لكتوز + +105 +00:07:59,360 --> 00:08:07,600 +فرمنتر الـ F الباقي + +106 +00:08:07,600 --> 00:08:12,080 +إيش مانو الباقي؟ أول حاجة إنهم لكتوز فرمنتر كلهم، + +107 +00:08:12,080 --> 00:08:14,420 +كلهم، سبع كهر الأب اللي هو الأحمر وليه غير الأحمر + +108 +00:08:15,080 --> 00:08:17,700 +لكن البعض .. non-lactose fermenters البعض منهم + +109 +00:08:17,700 --> 00:08:23,920 +normal والبعض منهم بقى فور مشي نقرأهم بشكل سريع + +110 +00:08:23,920 --> 00:08:28,340 +عشان يصيروا familiar ونبطل نتغلب في حفظهم طبعا + +111 +00:08:28,340 --> 00:08:31,160 +أنا بتأكد إنه صاروا familiar أصلا عندهم من المعنى + +112 +00:08:31,160 --> 00:08:36,460 +اللي نظر الشيليشيا شيجيلة إدوارد سيلى والكلمة + +113 +00:08:36,460 --> 00:08:39,600 +الكبيرة اللي ممكنش ننطقها بشكل جيد ونقطعها ده بس + +114 +00:08:39,600 --> 00:08:42,520 +إدوارد .. إدوارد اسمه معروف إدوارد سيلى + +115 +00:08:50,380 --> 00:08:54,780 +السيراتيا عارفينها السيراتيا الحابرة بروتيوس + +116 +00:08:54,780 --> 00:08:57,840 +بروفيدنسيا + +117 +00:08:57,840 --> 00:09:05,820 +مورجانيلا يرسينيا إيروينيا بكتينوباكتيريا بروتيوس + +118 +00:09:05,820 --> 00:09:12,740 +بروفيدنسيا مورجانيلا PPM PPM parts per million + +119 +00:09:16,200 --> 00:09:19,880 +هدول الـ PPM هم groups صحيح؟ group بمعنى إن هم .. + +120 +00:09:19,880 --> 00:09:22,960 +أنا أخلاق أنا قلت إنه كويس إن نحطهم مع بعض و + +121 +00:09:22,960 --> 00:09:26,800 +نسميهم PPM ده إن هم بيسموهم Protease group مجموعة + +122 +00:09:26,800 --> 00:09:30,440 +الـ Proteus والـ + +123 +00:09:30,440 --> 00:09:35,820 +Providentia والمورجانيلا بتميزوا + +124 +00:09:35,820 --> 00:09:40,940 +بميزة مهمة جدا اسمها Urease producers they produce + +125 +00:09:40,940 --> 00:09:45,320 +large amount of Ureasestrong urease producers + +126 +00:09:45,320 --> 00:09:52,320 +المصطلح الدقيق strong urease producers طيب عرفنا + +127 +00:09:52,320 --> 00:09:56,440 +بعض الأسماء في الـ enterobacteriaceae الآن نتكلم عن + +128 +00:09:56,440 --> 00:09:59,880 +شكل سريع عن الـ unclinic structure طبعًا الكلام اللي + +129 +00:09:59,880 --> 00:10:05,220 +على اللوحة ما فيش كلمة فيه جديدة كل الآن بدنا ندخل + +130 +00:10:05,220 --> 00:10:09,100 +لمرحلة التكرار مرحلة الاستفادة من المعلومات اللي + +131 +00:10:09,100 --> 00:10:11,880 +درسناها لما تكلمنا عن الـ structure أو عن الـ + +132 +00:10:11,880 --> 00:10:12,300 +metabolism + +133 +00:10:20,190 --> 00:10:25,430 +عشان نبدأ نتكلم في الـ medical microbiome مالذي + +134 +00:10:25,430 --> 00:10:27,590 +يميز الـ gram negatives الـ wall عن الـ gram + +135 +00:10:27,590 --> 00:10:30,090 +positives الـ wall؟ موجود أوتّر من بين، مضبوط لو + +136 +00:10:30,090 --> 00:10:32,050 +موجود أوتّر من بين ومن اللي مضمن اللي هو الـ + +137 +00:10:32,050 --> 00:10:37,210 +polysaccharide اللي هو الـ polysaccharide فاكرينه + +138 +00:10:37,210 --> 00:10:42,790 +اللي بيتقيل؟ الـ o-sided chain الـ o-sided chain و + +139 +00:10:42,790 --> 00:10:48,460 +الـ o-الـ H-antigen طبعًا أنا مرتبط الآن وجوده بمين؟ + +140 +00:10:48,460 --> 00:10:53,120 +بوجود الفلاجلة، لأن هو الفلاجلة الانتجين، إحنا لسه + +141 +00:10:53,120 --> 00:10:56,560 +قبل الحلقة ذكرنا، كنا F-motile، motile by + +142 +00:10:56,560 --> 00:11:01,560 +perifricous flagella، إذا البكتيريا متحركة، ففيها + +143 +00:11:01,560 --> 00:11:03,380 +flagella يعني فيها H-antigen + +144 +00:11:07,620 --> 00:11:13,280 +الـ H هو common لكل إجراء من الـ negative بس الـ + +145 +00:11:13,280 --> 00:11:16,700 +lie قاطبة انترو بكترياسي أو لا انترو بكترياسي + +146 +00:11:16,700 --> 00:11:20,120 +common لكن الـ H موجود فقط في تلك التي تحتاج + +147 +00:11:20,120 --> 00:11:24,520 +عليها اللي هو السمنة كذلك الأول بالنسبة للتالت + +148 +00:11:24,520 --> 00:11:29,900 +اللي هو الـ capsular antigen أو الـ K انتجن ويطلق + +149 +00:11:29,900 --> 00:11:32,700 +عليه لقب VI في حدث سمنة لا + +150 +00:11:39,210 --> 00:11:41,990 +فكان مسمين الكابسولة الكهربائي كهربائي كهربائي + +151 +00:11:41,990 --> 00:11:42,050 +كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي + +152 +00:11:42,050 --> 00:11:44,830 +كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي + +153 +00:11:44,830 --> 00:11:48,510 +كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي + +154 +00:11:48,510 --> 00:11:51,810 +كهربائي + +155 +00:11:51,810 --> 00:11:53,250 +كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي + +156 +00:11:53,250 --> 00:11:53,950 +كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي + +157 +00:11:53,950 --> 00:11:57,350 +كهربائي كهربائي كهربائي كهربائي كهربائي كهربائي + +158 +00:11:57,350 --> 00:12:09,110 +كهربائي كهربائي كهربائ + +159 +00:12:09,620 --> 00:12:12,080 +يعني الـ O أنتجين هي الدول الموجودة للـ O أنتجين + +160 +00:12:12,080 --> 00:12:18,340 +فإن + +161 +00:12:18,340 --> 00:12:21,840 +هو أنا معاه Antibody وبدي أكشف عن الـ O أنتجين في + +162 +00:12:21,840 --> 00:12:25,810 +الصورة هذه، ممكن أكشف عنه؟ لازم نشيل الـ capsule، + +163 +00:12:25,810 --> 00:12:30,110 +لازم نشيل الـ capsule، إذا وجود capsule will + +164 +00:12:30,110 --> 00:12:33,430 +interfere، إيش هالـ interfere؟ يتفاعل سلبًا، + +165 +00:12:33,430 --> 00:12:37,430 +هيمنعنا من الكشف عن الـ O-antigen، إذا بدنا .. بدنا + +166 +00:12:37,430 --> 00:12:41,430 +نلاقي طريقة نجشر، نشيل فيها الـ capsule، نمسك + +167 +00:12:41,430 --> 00:12:44,830 +المكشف اللي بطاطا ونمسك الـ bacteria اللي بدنا + +168 +00:12:44,830 --> 00:12:47,950 +نجشر فيها، ليش رأيكم؟ إحنا قادرين نمسكها الباتريا و + +169 +00:12:47,950 --> 00:12:54,710 +نشوفها أصلًا ففيها عملية تسخين لمدة نصف ساعة على + +170 +00:12:54,710 --> 00:12:58,310 +ستين درجة روية بتدمر اللي هو الـ capsule بتدمر الـ + +171 +00:12:58,310 --> 00:13:02,550 +capsule لإنه يبتكشف عن الـ O أنتجين فالكلام مرة + +172 +00:13:02,550 --> 00:13:07,370 +صورة اللي قدامنا فيها توضيح لثلاث أنواع من + +173 +00:13:07,370 --> 00:13:08,130 +الانتجينات + +174 +00:13:12,080 --> 00:13:15,160 +الـ Somatic Antigen أو الـ O Antigen وهو مكوّن من + +175 +00:13:15,160 --> 00:13:18,960 +الـ Lipid-A و Polysaccharide core و الـ O side + +176 +00:13:18,960 --> 00:13:22,300 +chain و الـ O Antigen بالإضافة إلى قلنا مين اللي + +177 +00:13:22,300 --> 00:13:28,020 +هو الـ Calcium طيب إحنا ذكرنا ثلاث انتيجينات + +178 +00:13:28,020 --> 00:13:33,060 +رئيسية OHK + +179 +00:13:33,060 --> 00:13:39,140 +هل هناك انتيجينات أخرى؟ نعم أقل أهمية لكن مين المهم + +180 +00:13:39,140 --> 00:13:43,530 +أكتر؟ جبنا إن هم التلات هدولة فهن مثلًا أعطر من + +181 +00:13:43,530 --> 00:13:48,150 +brain proteins كتيرة متنوعة برضه بتشكل كانتيجينات + +182 +00:13:48,150 --> 00:13:53,750 +داخل جسم الإنسان الـ pillar فاكرين الـ pillar في + +183 +00:13:53,750 --> 00:13:58,270 +محاضرة في محاضرة نيسيريا جونورية فقلناها بالخط + +184 +00:13:58,270 --> 00:14:02,390 +العريض لإنه كنا هي الأكثر يعني أو أكثر عامل شراسة + +185 +00:14:02,390 --> 00:14:08,130 +للبكتيريا واستغلت البكتيريا في تكوين أنواع مختلفة + +186 +00:14:08,130 --> 00:14:09,990 +صح؟ من الـ antigens + +187 +00:14:15,140 --> 00:14:18,700 +بقولها some are encoded by plasmids يعني البعض + +188 +00:14:18,700 --> 00:14:22,320 +منها مش محمولة على كروموزومة وإنما محمولة على الـ + +189 +00:14:22,320 --> 00:14:26,080 +plasmid اللي طبعًا كانوا كدرسين للمتر بتعرف إنّها + +190 +00:14:26,080 --> 00:14:29,400 +extra chromosomal self-replicating أو + +191 +00:14:29,400 --> 00:14:37,340 +independently replicated نعني messenger طيب + +192 +00:14:37,340 --> 00:14:41,760 +قبل ما أقفز على النواحي هذه بدنا نحاول ناخد بعض + +193 +00:14:44,370 --> 00:14:48,130 +المعلومات اللي قد تفيدنا لماذا ندرس إحنا أصلًا اللي + +194 +00:14:48,130 --> 00:14:50,890 +هو antigens ولا يوجد انتجين أو capsule انتجين + +195 +00:14:50,890 --> 00:14:55,310 +دايمًا المعلومات المعلومات أي نوع من المعلومات + +196 +00:14:55,310 --> 00:14:58,490 +موجودة قدامنا على الطاولة لو كان في عندي واحد + +197 +00:14:58,490 --> 00:15:03,630 +فيزيائي واحد كيميائي واحد طبيب واحد مريض واحد من + +198 +00:15:03,630 --> 00:15:08,670 +مجال مختلف كل واحد بينظر للمعلومة من وجه نظرّه + +199 +00:15:08,670 --> 00:15:12,830 +الخاصة يعني لو كنا ما فاقت الانتيجينات هنقول من وجه + +200 +00:15:12,830 --> 00:15:19,110 +نظر مين؟ المريض ولا البكتيريا ولا التشخيص اللي هو الـ + +201 +00:15:19,110 --> 00:15:22,050 +medical microbiology ولا الطبيب كل واحد اللي هو + +202 +00:15:22,050 --> 00:15:26,870 +أهداف مختلفة من المعلومة المتعلقة بمين؟ بالانتجين + +203 +00:15:26,870 --> 00:15:32,450 +إحنا نبدأ بحالنا microbiologist إيش بنستفيد من + +204 +00:15:32,450 --> 00:15:37,510 +الانتجين؟ تقول إيه كلها؟ إيش قلت عنها قبل شوية + +205 +00:15:37,510 --> 00:15:42,890 +أنت؟ موجودة بشكل natural فلقا معاها طب لو قلتلكوا + +206 +00:15:42,890 --> 00:15:45,670 +أنا أيكوا والله one five seven eight seven .. أي + +207 +00:15:45,670 --> 00:15:49,270 +كم مرة حكيتها هي اليوم اللي كانت؟ كتير صح؟ سابقين؟ + +208 +00:15:49,270 --> 00:15:52,130 +تلقى معاها برضه؟ طيب لو عزلنا .. إذا أنا واحد + +209 +00:15:52,130 --> 00:15:55,690 +معاه أسهل وعزلنا من الـ stool تبقى أي شي ليه شك + +210 +00:15:55,690 --> 00:16:00,030 +والله .. ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون + +211 +00:16:00,030 --> 00:16:02,990 +نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ + +212 +00:16:02,990 --> 00:16:03,390 +ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن + +213 +00:16:03,390 --> 00:16:03,450 +نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ ممكن تكون نوم؟ + +214 +00:16:03,450 --> 00:16:04,230 +ممكن تكون نوم + +215 +00:16:09,080 --> 00:16:12,060 +الـ Strain أو السلالة more specifically اتعرفنا + +216 +00:16:12,060 --> 00:16:15,860 +عليها الآن المرحلة اللي نقلناها للطبيب إيش فيها من + +217 +00:16:15,860 --> 00:16:21,700 +هو الـ body pathogen إنّها مش normal flora بيس يعني + +218 +00:16:21,700 --> 00:16:26,580 +أنا استفدت في المختبر إن اتعرفت بشكل أدق على بنا + +219 +00:16:26,580 --> 00:16:31,720 +الـ species E coli and strain E coli O157H7 نحن + +220 +00:16:31,720 --> 00:16:37,530 +مثال ثاني في أواخر التسعينات للقرن الماضي صار لنا + +221 +00:16:37,530 --> 00:16:43,810 +وضع كلها في غزة يعني كلها .. كلها .. الآن لو أنا + +222 +00:16:43,810 --> 00:16:46,630 +بتعمل investigation للـ source كلكم بتعرفوا إن كلها + +223 +00:16:46,630 --> 00:16:49,650 +تنتقل عن طريق الـ water pollution يعني ماء ملوثة + +224 +00:16:49,650 --> 00:16:51,870 +المياه .. مش كلها مصدر + +225 +00:16:56,530 --> 00:17:01,010 +من بلديات، وحتى البلدية الواحدة في المدينة الواحدة + +226 +00:17:01,010 --> 00:17:08,870 +قد تزود السكان بمياه ليست مصدر واحد، يعني هما + +227 +00:17:08,870 --> 00:17:10,930 +كانوا مصادر مختلفة ومتنوعة، يعني حتى المنطقة اللي + +228 +00:17:10,930 --> 00:17:16,810 +كانت غزة كبيرة جالها المنطقة مثلًا المخيم أو كده، + +229 +00:17:16,810 --> 00:17:20,940 +ففي النهاية أنا بدي أعرف مصدر الماء الملوث لا يعني + +230 +00:17:20,940 --> 00:17:26,220 +إن أنا عزلت Vibrio من ما يجهي نفس الـ Vibrio لعمل + +231 +00:17:26,220 --> 00:17:33,380 +الوباء لازم أعرف الانتيجينات للبكتيريا اللي معزولة + +232 +00:17:33,380 --> 00:17:38,100 +من الوضع من المرضى يعني واعرف الانتيجينات اللي + +233 +00:17:38,100 --> 00:17:42,800 +معزولة من العينات البيئية بها من ضمنها إنّها إذا + +234 +00:17:42,800 --> 00:17:48,140 +توافقت عينات كانتيجينات اللي معزولة من البيئة مع + +235 +00:17:48,140 --> 00:17:52,480 +تلك اللي توزنت من المرضى فبيقول هذا most likely is + +236 +00:17:52,480 --> 00:17:54,320 +a source of this disease + +237 +00:17:58,180 --> 00:18:02,780 +أو نوحي للناس بإنهم يستخدموا أو بنكلوا إحنا بنقول + +238 +00:18:02,780 --> 00:18:05,800 +بإجراءات مثلًا بنكلوا هذا الماء اللي بنحط عليه + +239 +00:18:05,800 --> 00:18:09,360 +الكلور يعني بناخد إجراء بين عالمين على مالماتنا من + +240 +00:18:09,360 --> 00:18:13,280 +الانتجار هذا الشيء نسميه هنا source tracking إيش + +241 +00:18:13,280 --> 00:18:17,200 +أنا source tracking؟ track تتبقى source المصدر + +242 +00:18:17,200 --> 00:18:20,920 +يبقى تبقى المصدر في المستشفى .. في المستشفى صاروا + +243 +00:18:20,920 --> 00:18:24,720 +بقى في أحد الأقسام والله افترض الحضارة على أحد + +244 +00:18:24,720 --> 00:18:28,440 +الأسافر اللي بيحدث بعض الأحيان، في الـ nursery، + +245 +00:18:28,440 --> 00:18:31,340 +حبارة اللي هم بيحطوا فيها الأطفال الحديثين الولادة + +246 +00:18:31,340 --> 00:18:33,780 +اللي هم غالبًا الخدد اللي يقولوا إنهم premature + +247 +00:18:33,780 --> 00:18:39,720 +babies، أطفال اللي أعمارهم حمير أقل من 9 شهور أو + +248 +00:18:39,720 --> 00:18:44,020 +اللي يقولوا إنهم عندهم إشكالية، فصار النوبة + +249 +00:18:44,020 --> 00:18:48,560 +ببكتيريا X، الآن بكتيريا X زي Staphylococcus مثلًا + +250 +00:18:48,560 --> 00:18:53,160 +أو السيرافية جينا بنعرف مصدر تبعها خدنا من العاملين + +251 +00:18:53,160 --> 00:18:56,180 +لإجينا سيراتيا و سيراتيا و سيراتيا بس ماجاش عندك + +252 +00:18:56,180 --> 00:18:59,360 +أنت سيراتيا أنت مش متهانة خالص لكن ماجاش تهيام + +253 +00:18:59,360 --> 00:19:03,520 +واحد اتنين تلاتة ما عندهم سيراتيا تفهم إلا إلا + +254 +00:19:03,520 --> 00:19:07,040 +اتأكدنا أن السيراتيا اللي عزلناها من المريض هي نفس + +255 +00:19:07,040 --> 00:19:10,100 +السيراتيا اللي عزلناها ولما بقينا نعرف عن طريق + +256 +00:19:10,100 --> 00:19:13,960 +غالباً الانتجينات نعرف الانتجينات بتاعت البقية إيه + +257 +00:19:13,960 --> 00:19:17,460 +ده Source tracking، Source tracking is very + +258 +00:19:17,460 --> 00:19:21,310 +importantميزنا في المثال الأول ميزنا بين Pathogen + +259 +00:19:21,310 --> 00:19:25,150 +و Non pathogen لما قولنا إيه شريش يا كولاي السؤال + +260 +00:19:25,150 --> 00:19:28,010 +على طول كان So what ماهي Natural لك لما قولنا إيه + +261 +00:19:28,010 --> 00:19:32,450 +كولاي O157H7 يبقى ده أنا صافيا معرفة بين أن هذا + +262 +00:19:32,450 --> 00:19:38,270 +السؤال هي Pathogen إذا بنا نفهم إنه احنا بالنسبة + +263 +00:19:38,270 --> 00:19:43,170 +إنه في الـ Microbiology نتعرف على الـ .. الـ .. الـ .. + +264 +00:19:47,020 --> 00:19:55,040 +أدق لهوية الكائن لأسباب متنوعة مختلفة + +265 +00:19:55,040 --> 00:20:01,240 +الـ Enterobacteriaceae ممكن احنا جوناها يعني محترمة في + +266 +00:20:01,240 --> 00:20:03,660 +الـ Intestinal tract أو مواطنة محترمة في الـ + +267 +00:20:03,660 --> 00:20:07,260 +Intestinal tract موجودة بشكل طبيعي بالعكس يعني مش + +268 +00:20:07,260 --> 00:20:09,740 +بس محترمة لها دور كويس في الـ Intestinal tract + +269 +00:20:14,560 --> 00:20:19,040 +لا تعوي ولا إنها Normal microbiota ستقوم بعمل + +270 +00:20:19,040 --> 00:20:23,660 +Infection وأهم واحدة انزل تحت أول واحدة من تحت + +271 +00:20:23,660 --> 00:20:27,540 +You Urinary tract infection لو تعرف أن E.coli + +272 +00:20:27,540 --> 00:20:30,540 +هي Number one cause of Urinary tract infection + +273 +00:20:33,510 --> 00:20:37,130 +لقيها Number one في شغل أكتر من شغل بس من ضمنها It + +274 +00:20:37,130 --> 00:20:41,690 +May cause Septicemia، It may cause Pneumonia، It may + +275 +00:20:41,690 --> 00:20:45,530 +cause Meningitis ولا الصحيح اللوحي دي مابدى نفهم + +276 +00:20:45,530 --> 00:20:48,730 +منها إن بس هدول هم اللي بيسبوا أوراق ولا مابدى + +277 +00:20:48,730 --> 00:20:50,950 +نفهم إن بس هدول هم الأوراق اللي بيسبوا هم هدول + +278 +00:20:50,950 --> 00:20:54,910 +المجموعة بس هتعطينا نموذج بقى إنه Outside the + +279 +00:20:54,910 --> 00:20:58,760 +Intestinal tract They may cause disease ومنها إنه هو + +280 +00:20:58,760 --> 00:21:01,600 +خطير قد يصل إلى اللي هقولنا الـ Meningitis و + +281 +00:21:01,600 --> 00:21:03,880 +الـ Cysticemia والـ Pneumonia والـ Urinary tract + +282 +00:21:03,880 --> 00:21:06,640 +Infection طب حتى الـ Urinary tract infection في + +283 +00:21:06,640 --> 00:21:10,120 +منها الـ Pyelonephritis والـ Salpingitis is a very + +284 +00:21:10,120 --> 00:21:13,280 +dangerous one وبالتالي بدنا نكون عارفين إن الـ UTI + +285 +00:21:13,280 --> 00:21:15,680 +مش حاجة سهلة طيب + +286 +00:21:18,110 --> 00:21:21,890 +الـ Enterobacteriaceae سنة قولت إنه نجسمها جسمين، جسم كان + +287 +00:21:21,890 --> 00:21:25,910 +Reside normally، يعني هو Normal flora، وجسم آخر + +288 +00:21:25,910 --> 00:21:28,710 +كان Cause infection في الـ Gas في الـ Intestine، من + +289 +00:21:28,710 --> 00:21:31,830 +الـ Intestine .. طبعاً كل الـ Intestine اللي كانوا موالين + +290 +00:21:31,830 --> 00:21:34,590 +باللون البرني فاكرينهم؟ رفارامين + +291 +00:21:37,900 --> 00:21:42,880 +مقنع خوفينا هناك لإنه احنا يعني موزعين بين نسميها + +292 +00:21:42,880 --> 00:21:46,720 +Normal flora ولا البعض .. البعض تلاتة منها اللي + +293 +00:21:46,720 --> 00:21:51,320 +بتسبب Infection نسميها Pathogen فهي الآن صرنا بحق + +294 +00:21:51,320 --> 00:21:55,320 +إنه لازم نجوانها Pathogen في الـ Intestinal tract + +295 +00:21:55,320 --> 00:21:58,660 +Salmonella، Shigella، Yersinia، Entero، Coelum + +296 +00:22:01,160 --> 00:22:03,860 +بنعرف إن الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +297 +00:22:03,860 --> 00:22:03,900 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +298 +00:22:03,900 --> 00:22:03,960 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +299 +00:22:03,960 --> 00:22:04,620 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +300 +00:22:04,620 --> 00:22:05,120 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +301 +00:22:05,120 --> 00:22:05,800 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +302 +00:22:05,800 --> 00:22:06,380 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +303 +00:22:06,380 --> 00:22:11,360 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +304 +00:22:11,360 --> 00:22:15,940 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +305 +00:22:15,940 --> 00:22:16,260 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +306 +00:22:16,260 --> 00:22:16,340 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +307 +00:22:21,210 --> 00:22:25,490 +وجود أي سيروتاعو .. وجود أي سيراتيا أو السلمونيلا في + +308 +00:22:25,490 --> 00:22:28,390 +الغداء يجعل هذا الغداء غير صالح للاستهلاك الآدمي + +309 +00:22:28,390 --> 00:22:36,370 +ما نحاكي عليه على الإطلاق السلمونيلا Pathogenic وجودها + +310 +00:22:36,370 --> 00:22:41,810 +.. وجودها يجعل الغداء والشراب والطعام أيًا كان غير + +311 +00:22:41,810 --> 00:22:47,070 +صالح للاستهلاك الآدمي السلمونيلا العقب اللي + +312 +00:22:47,070 --> 00:22:49,930 +هحكيلكوا هو إنه نبذة صغيرة جابنا وصلنا عن + +313 +00:22:49,930 --> 00:22:56,130 +السلمونيلا وعن الشيجيلة، هم لما بنقول سلمونيلا أصلاً + +314 +00:22:56,130 --> 00:23:01,460 +بنقول شيجيلة، سلمونيلا And شيجيلة Both has very + +315 +00:23:01,460 --> 00:23:04,380 +similar characteristics أو يعني Very common + +316 +00:23:04,380 --> 00:23:07,760 +characteristics منها إنهم Gram-negative بسلعي، + +317 +00:23:07,760 --> 00:23:09,900 +They are Enterobacteriaceae وكلمة Enterobacteriaceae + +318 +00:23:09,900 --> 00:23:12,800 +يعني كل الصفات المشتركة موجودة، They are non + +319 +00:23:12,800 --> 00:23:16,420 +-lactose fermenter، Non-lactose fermenter، لكن + +320 +00:23:16,420 --> 00:23:19,520 +بتبعتنا بسبب Intestinal tract infection، لكن + +321 +00:23:19,520 --> 00:23:23,360 +برضه هناك أوجه اختلاف كبيرة، كبيرة، كبيرة، كبيرة، + +322 +00:23:23,360 --> 00:23:26,960 +متعاطف الـ Disease الشيجيلة Has never been isolated + +323 +00:23:26,960 --> 00:23:30,580 +from sites other than the Intestinal tract بمعنى + +324 +00:23:30,580 --> 00:23:34,420 +أنه لم .. ما بتسيبش غير الـ Intestinal tract بس + +325 +00:23:34,420 --> 00:23:37,340 +السلمونيلا ممكن أجلبها من One infection، من Urinary + +326 +00:23:37,340 --> 00:23:40,980 +tract، من الـ Blood، من CSF، من أماكن مختلفة في جسم + +327 +00:23:40,980 --> 00:23:45,800 +الإنسان، أمام؟ هذا واحد رقم اتنين يعني بمعنى آخر + +328 +00:23:45,800 --> 00:23:50,420 +أنه الشيجيلة بتسيب One disease اللي هو اللي هو + +329 +00:23:50,420 --> 00:23:53,000 +Intestinal tract infection بس Salmonella بتسبب + +330 +00:23:53,000 --> 00:23:56,140 +بالإضافة إلى Intestinal tract infection أمور أخرى + +331 +00:23:56,140 --> 00:24:03,360 +كثيرة الشيجيلة ما عرفناهاش غير في الإنسان ما تعرفش يعني + +332 +00:24:03,360 --> 00:24:08,760 +مخلوق آخر يصاب بالشيجيلة غير الإنسان يعني نظرياً لو + +333 +00:24:08,760 --> 00:24:12,640 +دورنا على كل البشر اللي مصابين بالشيجيلة لو علاجناهم + +334 +00:24:12,640 --> 00:24:16,740 +سيأخذ على مين؟ على الشيجيلة نفسه على الدزيسة على + +335 +00:24:16,740 --> 00:24:23,110 +الشيجيلة نظرياً بينما السلمونيلا وجدت من العوائل + +336 +00:24:23,110 --> 00:24:29,470 +نسميها واحدة Reservoir، مخازن، من العوائل ما يعني + +337 +00:24:29,470 --> 00:24:36,270 +لا تتخيله، شفت الحردون، السحلية، التمساح، السلاحف، + +338 +00:24:36,270 --> 00:24:41,510 +الدواجن، البيض اللي احنا ممكن ناكله ممكن يكون + +339 +00:24:41,510 --> 00:24:45,770 +جواه سلمونيلا المؤامرة بينها وبين الدواجن كبيرة يا + +340 +00:24:45,770 --> 00:24:50,250 +بنات ووصلت لإن الدواجن تسمح للسلمونيلا تروح الـ + +341 +00:24:50,250 --> 00:24:55,030 +Ovary وتحقر البيض بخلية أو خليتين من السلمونيلا + +342 +00:24:55,030 --> 00:25:01,190 +وبالتالي أنتم عارفين البيض الآن بيدخل كمنتج في + +343 +00:25:01,190 --> 00:25:07,490 +شغلات كتيرة صح ولا لأ طب الدواجن جنة وفيها + +344 +00:25:07,490 --> 00:25:14,580 +سلمونيلا الدواجن الآن بنطبخها احنا والطبخ بتاعنا + +345 +00:25:14,580 --> 00:25:19,140 +مش على هوا البارد بنسخنها بظبط، بنسخنها أما + +346 +00:25:19,140 --> 00:25:22,520 +بالشواية أو بالقلي أو بالسلق، بالغلي، بالغلي + +347 +00:25:22,520 --> 00:25:26,080 +الميه وهذه غالباً الحرارة بتكون كافية لقتل + +348 +00:25:26,080 --> 00:25:30,920 +السلمونيلا إذا الدجاجة نفسها اللي أكلناها حتى لو + +349 +00:25:30,920 --> 00:25:34,000 +كان فيها سلمونيلا، غالباً We are protected إلا إذا + +350 +00:25:34,000 --> 00:25:37,140 +كان Undercooked، Undercooked يعني مقلي لم تتعرف + +351 +00:25:37,140 --> 00:25:39,780 +لدرجة حرارة كويسة، وأنا أتكلم في الشواية وفي + +352 +00:25:39,780 --> 00:25:40,860 +القلي، غالباً + +353 +00:25:44,080 --> 00:25:47,720 +السطح بتكون عالية لكن في الـ Center بتكون .. عشانك + +354 +00:25:47,720 --> 00:25:51,280 +لو بقى بتفتح حرطة لو لسه .. لسه بنزل دم مش .. يعني + +355 +00:25:51,280 --> 00:25:55,580 +مش مستوية الـ .. طب وين المشكلة؟ المشكلة في المطبخ + +356 +00:25:55,580 --> 00:26:00,820 +واحدة من القطة في اللحم تمام بيحدث ما يسمى Cross + +357 +00:26:00,820 --> 00:26:04,680 +contamination السكين اللي استخدمناها والخشب + +358 +00:26:04,680 --> 00:26:08,000 +التقطيع الـ Chopping board اللي استخدمناها هذه الآن + +359 +00:26:08,000 --> 00:26:11,750 +أصبحت عليها سالمونيلا احنا أخذنا الجولة وحطيناها في + +360 +00:26:11,750 --> 00:26:15,290 +الغابة، ما مات سبن اللي هناك بس إنها لها .. لها + +361 +00:26:15,290 --> 00:26:18,950 +سكينة ولها Chopping board ما ماتر، غسلت السكينة + +362 +00:26:18,950 --> 00:26:22,090 +لسه ليك خفيف أو الـ Chopping board ما غسلتيها كويس + +363 +00:26:22,090 --> 00:26:27,890 +وجيت وعملت بقدونس ولا شو .. يعني أي نوع من الأغدى + +364 +00:26:27,890 --> 00:26:31,990 +اللي .. اللي لا .. لا يطهر، يعني سلطات، تمام؟ + +365 +00:26:31,990 --> 00:26:36,770 +فأخذنا السلمونيلا .. أخذنا السلمونيلا من الـ .. الـ + +366 +00:26:36,770 --> 00:26:39,910 +.. الـ Chopping board أو من السكين وحطيناها في غذاء + +367 +00:26:39,910 --> 00:26:43,390 +لن يتعرض لحرارة عالية بتاعي سينقذ لنا السلمونيلا، + +368 +00:26:43,390 --> 00:26:46,570 +هذا بيسميها حالة Cross contamination وهذه الأخطر + +369 +00:26:46,570 --> 00:26:53,270 +الصحيح في السلمونيلا، هذا كان اليوم محاضرة تثقيفية + +370 +00:26:53,270 --> 00:26:53,850 +في المطبخ + +371 +00:26:56,610 --> 00:27:00,030 +بس هذا .. هذا الكلام .. هذا الكلام بيحتم علينا إنه + +372 +00:27:00,030 --> 00:27:04,770 +دايماً فصل الخضروات والـ .. الـ .. عن اللحوم، يعني + +373 +00:27:04,770 --> 00:27:07,430 +أي شيء متعمد فيه في الـ .. في المطبخ، خضروات علينا + +374 +00:27:07,430 --> 00:27:11,950 +نفصلها عن اللحوم والأسماك وما شابه ده يعني، + +375 +00:27:11,950 --> 00:27:15,670 +فـ E.coli، سلمونيلا، شيجيلا، يرسينيا، انترو، + +376 +00:27:15,670 --> 00:27:16,050 +كل شيء + +377 +00:27:19,320 --> 00:27:22,660 +الآن الـ Enterobacteriaceae بدنا ننظر إليها من + +378 +00:27:22,660 --> 00:27:27,080 +منظور كمان مرة إن حتى في الـ community في المجتمع + +379 +00:27:27,080 --> 00:27:32,780 +ممكن نروح المستشفى احنا بأمراض اكتسبناها من + +380 +00:27:32,780 --> 00:27:37,480 +المجتمع بتكون نتيجة عن الـ Enterobacteriaceae مثلا + +381 +00:27:37,480 --> 00:27:42,560 +Klebsiella pneumoniae Klebsiella pneumoniae بتسبب من + +382 +00:27:42,560 --> 00:27:50,430 +اسمها Pneumonia وطبعا بتسبب Pneumonia وإليها مؤهل + +383 +00:27:50,430 --> 00:27:53,170 +للـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +384 +00:27:53,170 --> 00:27:53,190 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +385 +00:27:53,190 --> 00:27:53,390 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +386 +00:27:53,390 --> 00:27:54,250 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +387 +00:27:54,250 --> 00:27:55,790 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +388 +00:27:55,790 --> 00:27:55,810 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +389 +00:27:55,810 --> 00:27:57,910 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +390 +00:27:57,910 --> 00:27:58,990 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +391 +00:27:58,990 --> 00:28:00,790 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +392 +00:28:00,790 --> 00:28:01,730 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +393 +00:28:01,730 --> 00:28:02,830 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +394 +00:28:02,830 --> 00:28:08,990 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +395 +00:28:08,990 --> 00:28:17,210 +الـ .. الـ .. + +396 +00:28:20,240 --> 00:28:25,940 +Polysaccharide Capsule الورداني ترد انفكشن واللي + +397 +00:28:25,940 --> 00:28:28,860 +بتنتج عن غرب على الفيكال Contamination could be + +398 +00:28:28,860 --> 00:28:35,600 +caused by E.coli E.coli + +399 +00:28:35,600 --> 00:28:39,240 +Klebsiella Enterobacter and Proteus البروتياس إلها + +400 +00:28:39,240 --> 00:28:42,560 +ميزة على الواقع فاكرين قبل شوية احنا قولنا بروتياس + +401 +00:28:42,560 --> 00:28:47,460 +جروب PPM بروتياس مورجانيلا و البروفيدنشيا They are + +402 +00:28:47,460 --> 00:28:52,540 +all strong Ureasestrong urea is يعني they can + +403 +00:28:52,540 --> 00:28:57,360 +convert urea to ammonia and CO2 ammonia is + +404 +00:28:57,360 --> 00:29:02,180 +alkaline يبقى الـ urine .. الـ urine عند الناس + +405 +00:29:02,180 --> 00:29:07,540 +المصابين بالبروتيا هيكون الـ pH تبعهم alkaline لما + +406 +00:29:07,540 --> 00:29:09,840 +تدرسوا الـ urine analysis انتوا ان شاء الله هتعرفوا + +407 +00:29:09,840 --> 00:29:13,740 +ان الـ pH تبع الـ urine بيقاس بنجيس الـ pH تبع الـ + +408 +00:29:13,740 --> 00:29:15,920 +urine بيفرق لما يكون acidic على neutral لما يكون + +409 +00:29:15,920 --> 00:29:19,660 +ايه؟ alkaline بالـ strip بالـ strip صحيح + +410 +00:29:27,060 --> 00:29:33,500 +الآن الـ pH تبع الـ urine is essential for some + +411 +00:29:33,500 --> 00:29:34,960 +salts + +412 +00:29:37,510 --> 00:29:42,130 +اللي احنا عشان بسميهم احنا الأملاح to precipitate + +413 +00:29:42,130 --> 00:29:45,030 +في البعض منهم بيصير الـ precipitation على high pH + +414 +00:29:45,030 --> 00:29:48,050 +والبعض الآخر بيصير الـ precipitation على low pH + +415 +00:29:48,050 --> 00:29:51,730 +فبالتالي لو كان الأملاح دي موجودة و الـ pH alkaline + +416 +00:29:51,730 --> 00:29:55,130 +عمال الترسيب بتكون واحدة وبالتالي تكوين حصوات أصلا + +417 +00:29:55,130 --> 00:29:59,310 +ككانوكيا بتكون أيضا يعني فنقدر نقول إن الـ Proteus + +418 +00:29:59,310 --> 00:30:04,130 +قد تساهم في تكوين بعض أنواع الـ stones في الـ + +419 +00:30:04,130 --> 00:30:05,910 +urinary tract + +420 +00:30:08,950 --> 00:30:13,050 +ولا مرة ولا بأي حال من الأحوال اللوحة اللي قدامنا + +421 +00:30:13,050 --> 00:30:18,330 +هي فقط التدليل على ان الـ antibacterial بتسبب + +422 +00:30:18,330 --> 00:30:21,710 +community acquired infections اقولكوا من أشهر + +423 +00:30:21,710 --> 00:30:24,090 +community acquired infections wound infections + +424 +00:30:25,070 --> 00:30:29,090 +تمام؟ بتعرفوا أطفال إن احنا بنحبوا فصل الشتاء و + +425 +00:30:29,090 --> 00:30:33,370 +بيكفّوه لما تكون الشرارة لسه يعني مليئة بـ الميه لكن + +426 +00:30:33,370 --> 00:30:37,350 +للأسف الـ sewage system انا في قطاع درست زي شفته + +427 +00:30:37,350 --> 00:30:41,810 +يعني في شوية أنطار بسيطة يعني صار في بعض المناطق + +428 +00:30:41,810 --> 00:30:45,830 +فيها يعني عدم قدرة على الأنظمة الـ sewage من + +429 +00:30:45,830 --> 00:30:49,730 +الستوار و لتاني صار فينا طوفان في بعض المناطق فلما + +430 +00:30:49,730 --> 00:30:54,500 +الأطفال تمشي في الـ .. الـ .. هذه المياه اللي الآن + +431 +00:30:54,500 --> 00:30:57,500 +اختلطت بالـ sewage اللي من ضمن الـ sewage stool .. + +432 +00:30:57,500 --> 00:31:01,080 +stool يعني انتروه بكترياسي من ضمنها إيشيريشيا كولاي + +433 +00:31:01,080 --> 00:31:04,280 +لو عنده اي scratch في الـ .. في الـ .. في المناطق + +434 +00:31:04,280 --> 00:31:06,880 +اللي اتعرضت للماء ممكن يصيب عنده wound infection + +435 +00:31:06,880 --> 00:31:10,100 +wound infection إذا لان بيبقى عالم ممكن يصيب عنده + +436 +00:31:10,100 --> 00:31:13,530 +septicemia إذا لم يعرفوا إنهم لديهم meningitis + +437 +00:31:13,530 --> 00:31:17,690 +فنعم يعني بمعنى آخر إن الـ .. الـ .. الـ + +438 +00:31:17,690 --> 00:31:21,970 +Enterobacteriaceae مصدر مهم للعدوى في الـ community كما + +439 +00:31:21,970 --> 00:31:27,610 +هي مصدر مهم للعدوى في المستشفى نبدأ بالمايكروب + +440 +00:31:27,610 --> 00:31:33,790 +الأول اللي هو الـ إيشيريشيا كولاي نبدأ + +441 +00:31:33,790 --> 00:31:36,450 +بـ بعض الـ .. الـ .. + +442 +00:31:42,130 --> 00:31:45,390 +غير إنكم تستحضروا الاحترام والتقديم للبكتيريا، + +443 +00:31:45,390 --> 00:31:48,210 +إنكم تذكروا إن ما بتتكلم عن .. ما بتتكلم عن + +444 +00:31:48,210 --> 00:31:53,550 +سلالة، بتتكلم عن سلالات، قد واحدة منهم تحتوي على + +445 +00:31:53,550 --> 00:31:58,750 +واحد أو أكثر من هذه الأراسل، تمام؟ وقد تحتوي على + +446 +00:31:58,750 --> 00:32:03,810 +اتنين أو تلاتة، أربعة، حسب النوع اللي هو السلالة، + +447 +00:32:03,810 --> 00:32:09,990 +بتكونوا تعرفوا إن كل الـ Enterobacteriaceae بدون الـ + +448 +00:32:09,990 --> 00:32:15,670 +Lipopolysaccharide ما بتسوش حاجة بمعنى والكلام تم + +449 +00:32:15,670 --> 00:32:19,150 +تجذيبه إن في بعض الميكروبات أزيل منها الـ + +450 +00:32:19,150 --> 00:32:23,650 +Lipopolysaccharide فقدت قدرتها على الـ pathogenesis + +451 +00:32:23,650 --> 00:32:28,170 +فالـ Lipopolysaccharide هذا أمر مهم جدا اللي احنا + +452 +00:32:28,170 --> 00:32:32,610 +أخدنا من ضمنه أخدنا منه مين فقد الـ endotoxin في + +453 +00:32:32,610 --> 00:32:36,610 +هذه الحالة كبيرة بالنسبة لك لكن البكتيريا + +454 +00:32:37,700 --> 00:32:40,800 +طب .. طب بدي أخلص على النص شوية في المحاضرة و + +455 +00:32:40,800 --> 00:32:45,720 +نحاول إن .. برضه نثبت بعض الـ .. أو مش نثبت .. + +456 +00:32:45,720 --> 00:32:50,660 +نحاول إن يعني نقرب بعض الأفكار اللي كانت غريبة + +457 +00:32:50,660 --> 00:32:56,300 +لزمان الشيشة كلها طول عمرها مسالمة، natural في الـ + +458 +00:32:56,300 --> 00:33:00,900 +intestinal flora فجأة لجناها منها سلالات بتنتج أو + +459 +00:33:00,900 --> 00:33:04,400 +تمال ديارية ولما دورنا على الـ toxin تبعها على + +460 +00:33:04,400 --> 00:33:09,520 +جناب بيشبه الشيجل، فسموه Shigella ومرة رأى تكسين + +461 +00:33:09,520 --> 00:33:14,000 +مشهور كتير اسمه LT toxin بيشبه الكوليرات، لحظة + +462 +00:33:14,000 --> 00:33:19,100 +بيشبه الكوليرات، بيشبه الشجلة، ففاكرين إن الفكرة + +463 +00:33:19,100 --> 00:33:24,300 +تبع إن gene transfer إن HGT إن ممكن + +464 +00:33:24,300 --> 00:33:27,720 +بكتيريا HGT لما بيقولوا Conjugation + +465 +00:33:27,720 --> 00:33:31,460 +غالبا بيقولوا Species لغير Species مع Species + +466 +00:33:31,460 --> 00:33:35,640 +بنفس الـ Species يعني يعني E.coli مع E.coli بس الآن + +467 +00:33:35,640 --> 00:33:40,020 +واضح من إن .. إن في .. إن في إمكانية لانتقال من + +468 +00:33:40,020 --> 00:33:43,320 +Genus لـ Genus من Genus لـ Genus يعني مثلا ممكن من + +469 +00:33:43,320 --> 00:33:47,300 +Shigella انتقل لمين؟ لـ Escherichia coli من + +470 +00:33:47,300 --> 00:33:54,370 +Salmonella لـ .. أو من Vibrio لـ Escherichia coli وإن + +471 +00:33:54,370 --> 00:34:00,070 +في ممكن مكان يلتقي فيه ميكروفات ومناسب و تتبادل مع + +472 +00:34:00,070 --> 00:34:03,450 +بعض الانتجنات، Swish systems، أنظمة المجال الـ + +473 +00:34:03,450 --> 00:34:06,190 +intestinal tract، تمام؟ لو واحد أصيب بسلامة من + +474 +00:34:06,190 --> 00:34:10,130 +الله، ما هو الـ E.coli موجودة، إمكانية إن يصير فيه + +475 +00:34:10,130 --> 00:34:13,590 +تبادل، الموقع بيكون عالي، قد تنجح وقد تفشل، لذلك + +476 +00:34:13,590 --> 00:34:19,250 +كل فترة وفترة بنلاقي سلالات جديدة من الـ E.coli يعني + +477 +00:34:19,250 --> 00:34:23,930 +جامد ممكن تلت سنوات طلعت سلالة في ألمانيا وصار + +478 +00:34:23,930 --> 00:34:27,850 +حتى يعني زعل من إسبانيا همت .. ألمانيا همت + +479 +00:34:27,850 --> 00:34:30,730 +إسبانيا إن من الـ .. طماطم المستوردة .. أو من + +480 +00:34:30,730 --> 00:34:33,690 +الخيار المستورد حتى المصريين أطلقوا عليها إعلاميا + +481 +00:34:33,690 --> 00:34:36,910 +بكتيريا الخيار، اه المصريين بيحبوا يـ .. لأ وفي + +482 +00:34:36,910 --> 00:34:42,210 +الآخر طلعت ملخ حراقة بالـ .. بإسبانيا، أوروبا + +483 +00:34:44,500 --> 00:34:49,540 +أوروبا مرتع لظهور صورة جديدة من اللي هو الـ إيشيريشيا + +484 +00:34:49,540 --> 00:34:51,920 +كولاي، احنا بالنسبة لإنها يمكن يكون مولود هناك + +485 +00:34:51,920 --> 00:34:56,240 +لإنها واخشة يعني، بس هم عندهم أدوات الـ diagnosis + +486 +00:34:56,240 --> 00:35:00,390 +دقيقة وبتالي بيكون في عندهم تتبع يبقى أنا اللي + +487 +00:35:00,390 --> 00:35:04,150 +بتواصله من الكلام الأخير هذا إن احتمالية انتقال + +488 +00:35:04,150 --> 00:35:06,830 +جينات من microbe لـ microbe واردة وهذا أحد أهم + +489 +00:35:06,830 --> 00:35:09,850 +الأسباب اللي دائما بتقولنا إن في الـ microbiology + +490 +00:35:09,850 --> 00:35:15,230 +مافيش عنها ثبات إن هي بتتغير باستمرار فمثلا عن + +491 +00:35:15,230 --> 00:35:20,210 +Toxins زي Shiga type toxin و enteroaggregative ST + +492 +00:35:20,210 --> 00:35:23,210 +like toxin ST يعني stable toxin like + +493 +00:35:27,240 --> 00:35:33,340 +Hemolysins, endotoxins, type 3 secretion system هو + +494 +00:35:33,340 --> 00:35:40,020 +عبارة عن نظام حقن بوصف اسمه molecular syringes + +495 +00:35:45,600 --> 00:35:51,220 +فهو عبارة عن سيرنج سيرنج أو مايعش بالـ syringe لكن صغيرة + +496 +00:35:51,220 --> 00:35:54,560 +جدا ده يسموها molecular syringes البكتيريا + +497 +00:35:54,560 --> 00:36:00,920 +بتستخدمها لحق مركبات إما في بكتيريا أخرى أو في + +498 +00:36:00,920 --> 00:36:07,560 +host cell المركبات هذه بتاع بقى تخيل معايا الـ E + +499 +00:36:07,560 --> 00:36:10,840 +.coli موجودة في الـ intestinal tract على enterocyte + +500 +00:36:10,840 --> 00:36:15,570 +على enterocyte خلية من خلال الـ intestinal tract ال + +501 +00:36:15,570 --> 00:36:19,250 +enterocyte مش معنية بإنها تعمل phagocytosis لل + +502 +00:36:19,250 --> 00:36:25,250 +الإشريشيا كولاي مش معنية، فالإشريشيا كولاي بتحقق أن + +503 +00:36:25,250 --> 00:36:28,910 +فيها مادة بتقولها .. بتوشوشها، المادة بتقولها زي + +504 +00:36:28,910 --> 00:36:33,410 +الإيبليسيا بتقولها: "يعني أنتِ حاجتك ناجس، طب ما أنتِ ما + +505 +00:36:33,410 --> 00:36:36,010 +شاء الله تمتلكين مقومات الـ macrophage ومبتملكيش + +506 +00:36:36,010 --> 00:36:40,780 +مقومات الـ phagocyte خالص، أنا غذاء جيد، تخيلوا + +507 +00:36:40,780 --> 00:36:45,640 +بكتيريا جاية خالية كليني، ايش معناها؟ eat me، ايش + +508 +00:36:45,640 --> 00:36:49,920 +معناها؟ أنّها بدها تدخل إلى الداخل، بدها تدخل إلى + +509 +00:36:49,920 --> 00:36:54,880 +الداخل، ولو عارفة حالها هتموت في الداخل، كان + +510 +00:36:54,880 --> 00:36:59,480 +مبادرهاش كليني، كان المبادر تـ exist intracellularly + +511 +00:37:00,510 --> 00:37:05,330 +فالآن ايش اللي بيصير؟ أنّ السيرنج بتحكم الخلية اللي + +512 +00:37:05,330 --> 00:37:09,930 +هو السلب بمواد تعمل حد يسموها polymerization of + +513 +00:37:09,930 --> 00:37:13,010 +أكتين، بتعمل أكتين، تخيلوا معايا الخلية بهذا الشكل + +514 +00:37:13,010 --> 00:37:18,950 +اللي مخليها واقفة ومنتفخة ومختل شكلها هو الـ + +515 +00:37:18,950 --> 00:37:22,650 +cytoskeleton، عارفين مما يتكون الـ cytoskeleton؟ من + +516 +00:37:22,650 --> 00:37:26,870 +الأكتين polymer، فبتروح تعمل polymerization للأكتين + +517 +00:37:26,870 --> 00:37:30,300 +where الخلية الموجودة هي الموجودة هنا، الخلية تخيلوا + +518 +00:37:30,300 --> 00:37:34,820 +البوطن هذه الصغيرة، تمام؟ فبيروح بالأكتين بيرفع + +519 +00:37:34,820 --> 00:37:38,140 +السجف منها، و بيرفع السجف منها، و بيصير عنده كذا + +520 +00:37:38,140 --> 00:37:42,640 +أقدام كاملة، والبكتيريا بتصير في النص، بيبلعها من + +521 +00:37:42,640 --> 00:37:48,620 +داخل إلى داخل زي الفجوزوم، وهي في الفجوزوم الآن، + +522 +00:37:48,620 --> 00:37:52,020 +هي موجودة هنا في الفجوزوم، لما البكتيريا تقوم في + +523 +00:37:52,020 --> 00:37:56,440 +الفجوزوم، ايش أول خطوة بيجي الآن؟ الـ lysosome + +524 +00:37:56,440 --> 00:38:00,830 +عشان يصير الفجوزوم الـ lysosome، على طول بتطلع الـ + +525 +00:38:00,830 --> 00:38:04,210 +syringe تبعها وبتحقن الـ membrane وبتقول + +526 +00:38:04,210 --> 00:38:09,910 +الفجوزوم false alarm، يعني جرس إنذار خاطر، أنت خليك + +527 +00:38:09,910 --> 00:38:14,590 +مطرحك، ما في عنا مشاكل، خليك مكانك، طبعًا لما + +528 +00:38:14,590 --> 00:38:18,330 +الفجوزوم ما يرتبطش .. يا عفوًا، الفجوزوم ما يرتبطش + +529 +00:38:18,330 --> 00:38:18,990 +في الفجوزوم +530 +00:38:22,190 --> 00:38:25,770 +بعد ذلك يبدأ + +531 +00:38:25,770 --> 00:38:30,010 +البكتيريا تنقسم داخل الخلية، انقسام البكتيريا داخل + +532 +00:38:30,010 --> 00:38:36,850 +الخلية، خد بالك، له معاني كثيرة في جهاز المناعة، بعيد + +533 +00:38:36,850 --> 00:38:37,150 +عن +534 +00:38:41,140 --> 00:38:45,980 +بعيد عن الـ antibodies، إذا في antibodies، لأن جوا + +535 +00:38:45,980 --> 00:38:48,320 +الخلية، الـ antibiotics بتدخل جوا الخلية، مش هتدخل جوا + +536 +00:38:48,320 --> 00:38:51,700 +الخلية، صح ولا لأ؟ تركيز الـ antibiotics لو في + +537 +00:38:51,700 --> 00:38:55,680 +antibiotics في الخلايا، أكيد هيكون أقل من تركيزه في + +538 +00:38:55,680 --> 00:39:01,940 +الدم، فهي محصورة نوعًا ما داخل الخلايا اللي بعتناها، و + +539 +00:39:01,940 --> 00:39:07,180 +تتكاثر، وتستخدم الخلية كمادة غذائية لها في التكاثر + +540 +00:39:07,180 --> 00:39:12,880 +إذا الـ type 3 secretion system نظام رائع جدا لخداع + +541 +00:39:12,880 --> 00:39:21,380 +الخلية، ومن ثم حق الملكبات، يعني لتطويع الـ host + +542 +00:39:21,380 --> 00:39:23,840 +للصلاة +543 +00:39:25,600 --> 00:39:29,460 +الـ .. الـ adhesins، وطلع منها أكثر من نوع من الـ .. + +544 +00:39:29,460 --> 00:39:33,820 +من الـ adhesins موجودين في الـ ecotype، وأن في حوالي + +545 +00:39:33,820 --> 00:39:39,860 +واحد وعشرين different types، أو الا كيف بدنا نفسر + +546 +00:39:39,860 --> 00:39:43,690 +.. كيف بدنا نفسر urinary tract infection، البكتيريا + +547 +00:39:43,690 --> 00:39:49,350 +دخلت من الـ urethra، من مكان منخفض، وبيطلع فوق عكس + +548 +00:39:49,350 --> 00:39:52,310 +التيار، التيار مين؟ الـ urethra اللي نزل تحت، صعبة + +549 +00:39:52,310 --> 00:39:55,390 +بقت، تطلع وتصل للكلية، وتعمل pyelonephritis، كيف + +550 +00:39:55,390 --> 00:39:59,370 +بينفسر الكلام؟ بوجود قدرة هائلة على الـ + +551 +00:39:59,370 --> 00:40:01,550 +attachment من قبل اللي هو البكتيريا اللي اسمها + +552 +00:40:01,550 --> 00:40:05,180 +Escherichia coli، الـ virulence factors that protect the + +553 +00:40:05,180 --> 00:40:07,980 +bacteria from most defenses زي الكبسول والـ iron + +554 +00:40:07,980 --> 00:40:10,920 +capturing ability، يعني عندها مقدرة أنّها تبرض + +555 +00:40:10,920 --> 00:40:14,760 +ترتبط بمين؟ بالحديد، ولا بيساعد على المنافسة معاها + +556 +00:40:14,760 --> 00:40:18,300 +اللي هو اللي هو أصلاً أيضًا في تبتلك outer membrane + +557 +00:40:18,300 --> 00:40:21,120 +proteins، إذا أنّ في مجموعة كبيرة من الـ virulence + +558 +00:40:21,120 --> 00:40:25,080 +factors موجودة في الإشريشيا كولاي، البعض بقرّنها وبعض + +559 +00:40:25,080 --> 00:40:28,880 +آخر حد هتكررها بالتفصيل، هتكرر كل نوع من الإشريشيا + +560 +00:40:34,010 --> 00:40:38,550 +بيجاوبنا على أسئلة كثيرة قد تساعدنا في فهم بعضنا + +561 +00:40:38,550 --> 00:40:42,230 +البعض +562 +00:40:42,230 --> 00:40:44,910 +أنا براحة قبل أنّ أحضر قطار، أنا حاطط فيديو على + +563 +00:40:44,910 --> 00:40:51,320 +الفيسبوك بمضحك الليستيريا، الليستيريا كيف تخترق + +564 +00:40:51,320 --> 00:40:57,060 +الخلية إلى خلية أخرى بدل أنّها تطلع برة، تمام؟ وتعمل + +565 +00:40:57,060 --> 00:41:00,360 +infection لخلية تانية؟ ده بيعمل حاجة اسمها actin + +566 +00:41:00,360 --> 00:41:04,180 +rockets، اللي هي صواريخ أكتين، وبتضرب حاجة بتروح + +567 +00:41:04,180 --> 00:41:08,860 +لها على الخلية التانية، ياريت تراجعوا الحسابات تبعنا في + +568 +00:41:08,860 --> 00:41:11,440 +الـ essential medical micro، وفي الفيسبوك لإني أنا + +569 +00:41:11,440 --> 00:41:15,100 +كل فترة فترة بضيف يعني بعض الأشياء الجاية المناسبة + +570 +00:41:15,480 --> 00:41:18,160 +هذه الصورة اللي كتبتها قبل هيك، اللي اتكلمنا عنه + +571 +00:41:18,160 --> 00:41:21,600 +اللي هو الارتباط بالمانوز والجالاكتوز بالنسبة + +572 +00:41:21,600 --> 00:41:27,120 +للفهم اللي هي type 1 و type B، هذه الصورة اللي + +573 +00:41:27,120 --> 00:41:31,280 +بتوضح أنّنا قلنا الـ .. الـ urinary tract infection + +574 +00:41:31,280 --> 00:41:35,480 +وقلنا في شكلين من الـ UTI اللي هو الـ cystitis وكل + +575 +00:41:35,480 --> 00:41:39,260 +كثير هو الـ cystitis، لما درسنا الـ Staphylococcus + +576 +00:41:39,260 --> 00:41:42,740 +saprophyticus اللي بتعمل cystitis اللي هي الـ UTI + +577 +00:41:44,600 --> 00:41:48,460 +لكن اللي أول مرة بنحكيله هو الـ pyelonephritis، وكلمة + +578 +00:41:48,460 --> 00:41:52,000 +بيولو جاية من كلمة pus، معناها الحكي أنّ الـ urine .. + +579 +00:41:52,000 --> 00:41:56,940 +الـ urine هنلاقيه مليان صديد .. مليان صديد، يعني في + +580 +00:41:56,940 --> 00:41:59,780 +معنى آخر، لما نعمل microscopic analysis of urine + +581 +00:42:06,920 --> 00:42:10,760 +واحد لتلاتة، ممكن تلاقي عشرين، ممكن تلاقي مية، + +582 +00:42:10,760 --> 00:42:15,600 +ممكن تلاقي full، بمعنى ما نستطيع حتى قراءة العينة، + +583 +00:42:15,600 --> 00:42:17,660 +ممكن تلاقي ما فيه خلايا صدرية + +584 +00:42:20,180 --> 00:42:22,960 +it is a leading cause of urinary tract infection + +585 +00:42:22,960 --> 00:42:26,860 +which can cause acute cystitis أو pyelo .. pyelo + +586 +00:42:26,860 --> 00:42:30,500 +.. pyelonephritis، إذا أنّ هي يعني من .. واحنا عملنا + +587 +00:42:30,500 --> 00:42:34,900 +حقيقة دراستين في غزة، أنا شخصيًا ساهمت فيهم، وأنا + +588 +00:42:34,900 --> 00:42:39,600 +شاركت فيهم، وفي الدراستين بالفعل وجدنا أنّ الـ E.coli + +589 +00:42:39,600 --> 00:42:47,480 +هي number one، ويعني من الـ .. الـ .. طبيعي أنّ ده جهة + +590 +00:42:47,480 --> 00:42:50,380 +number one في كل العالم تقريبًا، برضه كل الدول اللي + +591 +00:42:50,380 --> 00:42:54,480 +هنا فيانهم فيانهم طبعًا number two اختلف عن بعض + +592 +00:42:54,480 --> 00:42:58,860 +الدول، لكن number one هو بالفعل الاشريشيا كولاي + +593 +00:42:58,860 --> 00:43:06,040 +كمان leading cause، أنا بقول UTI كمان leading cause + +594 +00:43:06,040 --> 00:43:11,890 +of neonatal، مش أي meningitis، neonatal meningitis + +595 +00:43:11,890 --> 00:43:13,910 +leading cause of neonatal meningitis and + +596 +00:43:13,910 --> 00:43:17,090 +septicemia with a high mortality rate، يبقى نسبة + +597 +00:43:17,090 --> 00:43:21,190 +الوفيات في حالة الـ neonatal meningitis بتكون عالية + +598 +00:43:21,190 --> 00:43:25,170 +despite intensive treatment، حتى ورغم أنّنا بنعمل + +599 +00:43:25,170 --> 00:43:29,370 +علاج بالـ antibiotic، إلا أنّ الحالات + +600 +00:43:33,860 --> 00:43:37,740 +مميزات السلالة اللي بتعمل neonatal meningitis و + +601 +00:43:37,740 --> 00:43:41,900 +septicemia أنّها capsulated وبتحتوي على K1، فاكرين K1 + +602 +00:43:41,900 --> 00:43:43,880 +أو الـ K antigens اللي هو الكبسولات + +603 +00:43:56,380 --> 00:44:02,100 +الـ E.coli capsulated تكون عاملة large colonies و + +604 +00:44:02,100 --> 00:44:07,700 +mucoid colonies، تشبه مين؟ تشبه الـ Klebsiella + +605 +00:44:07,700 --> 00:44:14,920 +لذلك يا بنات، أنا بتحكي لكم قصة صغيرة توضح كيف احنا + +606 +00:44:14,920 --> 00:44:20,820 +مش لازم نعتمد على الشكل فقط، لغير في عملية التعرف + +607 +00:44:20,820 --> 00:44:25,370 +على البكتيريا، لما اشتغلت بالجامعة كان فينا دراسة وكان + +608 +00:44:25,370 --> 00:44:30,210 +عندنا عزل .. يعني بنعمل دراسة .. بنعمل يعني عزل + +609 +00:44:30,210 --> 00:44:36,870 +لميكروبات كثيرة من عينات PE، فالزملاء + +610 +00:44:36,870 --> 00:44:42,910 +اللي كانوا معانا في الـ .. في الـ .. في القسم يعني + +611 +00:44:42,910 --> 00:44:46,190 +بيقولوا: "طب هذه ليش بتاعتها الـ API بتاعتها الـ API + +612 +00:44:46,190 --> 00:44:50,030 +الـ environment identification system تبع البكتيريا + +613 +00:44:50,030 --> 00:44:52,850 +فبقول لهم: "ده أنّ أنا مش عارف هل هي E.coli ولا K + +614 +00:44:52,850 --> 00:44:55,170 +.pneumoniae ولا E.enterobacter، لأ واضح أنّها E.coli + +615 +00:44:55,170 --> 00:44:59,570 +واحنا هذا الرد بهم، واضح أنّها E.coli، هي K.pneumoniae + +616 +00:44:59,570 --> 00:45:08,060 +هي K.pneumoniae، فحتى أثبت اللي هم أخدت منهم تعريفاتهم + +617 +00:45:08,060 --> 00:45:10,880 +أنتِ بتقولي عنها هذا يعني رقم واحد، ايش بتقولي عنها + +618 +00:45:10,880 --> 00:45:13,440 +equalize، هذي ايش بتقولي عنها Klebsiella، هذي بتقول + +619 +00:45:13,440 --> 00:45:17,720 +عنها كذا كذا كذا، طبعًا سجلتهم وعملت لهم API، twenty + +620 +00:45:17,720 --> 00:45:23,600 +eight، تسعين بالمية راب كان الكلام كان غلط، يبقى + +621 +00:45:23,600 --> 00:45:27,640 +الاعتماد على الـ morphology لوحده، هذا هو السبب + +622 +00:45:27,640 --> 00:45:30,260 +اللي بوصلكم إليه، أنّ الاعتماد على الـ morphology + +623 +00:45:30,260 --> 00:45:35,860 +لوحده تمام، مش بيقولوا له أنّ سيء، لكن ليس هو + +624 +00:45:35,860 --> 00:45:40,010 +الأساس في التعرف، هو بساعدني في التعرف كـ clue + +625 +00:45:40,010 --> 00:45:45,630 +يعني مغزة أو كإشارة، لكن ليس هو التعرف الدقيق اللي + +626 +00:45:45,630 --> 00:45:49,010 +بيبقى نتم بيه الـ biochemical and physiological + +627 +00:45:49,010 --> 00:45:54,230 +testing إما بدونه صعب هاي شوفنا مثلا Klebsiella و + +628 +00:45:54,230 --> 00:45:56,390 +احنا فاكرين إن Klebsiella طلعت إشريشيا كلها والعكس + +629 +00:45:56,390 --> 00:46:02,370 +صحيح قد نجد بعض الأحيان إشريشيا انفكاش تطلع + +630 +00:46:02,370 --> 00:46:05,990 +Klebsiella وبالتالي كويس إنه نعمل على الأقل الـ + +631 +00:46:05,990 --> 00:46:10,760 +minimum biochemical test طيب المرة الجاي إن شاء + +632 +00:46:10,760 --> 00:46:14,420 +الله بنبدأ بالـ .. الميكروبات اللي متعلقة بالـ E + +633 +00:46:14,420 --> 00:46:16,440 +.coli ال تمام infection بالـ intestinal diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..af2b95ef65cf1a0d831528fd07c2aafe9850f3ae --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/5rnHqFi4ziI_postprocess.srt @@ -0,0 +1,2436 @@ +1 +00:00:20,670 --> 00:00:22,550 +بسم الله الرحمن الرحيم انا هتكلم اليوم موضوع جديد + +2 +00:00:22,550 --> 00:00:26,330 +ان شاء الله بعنوان bacterial vaccines هو الأنوان + +3 +00:00:26,330 --> 00:00:30,850 +جديد لكن انا متأكد المعلومات اللي هنشوفها ضمن هذا + +4 +00:00:30,850 --> 00:00:36,110 +ال chapter هتلاقي ان جزء الكبير منها تم دراسته كنا + +5 +00:00:36,110 --> 00:00:39,190 +بيعرف على سبيل المثال انه من الميكروبات اللي + +6 +00:00:39,190 --> 00:00:42,770 +دراسناهم Salmonella كنت prevented by ال vaccine + +7 +00:00:42,770 --> 00:00:47,860 +Shigella كنت prevented by ال vaccineكثير من + +8 +00:00:47,860 --> 00:00:50,300 +الأمراض اللي اضطررتنا إليها قلنا vaccine + +9 +00:00:50,300 --> 00:00:53,400 +preventable disease قلتلي هل أنا بعرفها في بعض + +10 +00:00:53,400 --> 00:00:57,820 +الأمراض هذه اليوم هحنحاول إنه نعمل categorization + +11 +00:00:57,820 --> 00:01:02,480 +عملية تصنيف لل vaccine و تعريف قلنا ال process + +12 +00:01:02,480 --> 00:01:06,710 +immunizationاللي هم نقولها دعاها بالتطاين او + +13 +00:01:06,710 --> 00:01:10,450 +التوقيع could be active و could be passive يعني + +14 +00:01:10,450 --> 00:01:14,970 +ممكن نعطي انتجينات و ممكن نعطي antibodies إذا + +15 +00:01:14,970 --> 00:01:18,930 +أعطينا انتجينات الجسم بيكون antibodies و أنا بسميه + +16 +00:01:18,930 --> 00:01:23,590 +active immunization إذا أعطينا ال universe جهزة + +17 +00:01:23,590 --> 00:01:27,870 +برا بيسميها passive immunization و الحالتين + +18 +00:01:27,870 --> 00:01:32,110 +مطموضين إنه نعمل active و نعمل passiveناخد أول + +19 +00:01:32,110 --> 00:01:36,130 +مثال أكتر immunity is induced by vaccines prepared + +20 +00:01:36,130 --> 00:01:39,070 +from bacteria or their products يبقى ال vaccines + +21 +00:01:39,070 --> 00:01:44,930 +في اللوحة هذه بتقول كلمتين مهمين إما ال vaccine هي + +22 +00:01:44,930 --> 00:01:48,710 +عبارة عن إما البكتيريا نفسها أو منتجاتها + +23 +00:01:48,710 --> 00:01:53,810 +والبكتيريا إما killed or attenuated يعني إما ميتة + +24 +00:01:53,810 --> 00:02:00,320 +أو مضاعفةأو منتجاتها ايش هنا منتجاتها؟ ممكن + +25 +00:02:00,320 --> 00:02:05,100 +proteins ممكن fragments من ال proteins او من + +26 +00:02:05,100 --> 00:02:09,300 +الخلية إذا في الآخر مش الخلية كلها و ده أجزاء من + +27 +00:02:09,300 --> 00:02:13,020 +الخلية ال passive زي ما حكينا it is preformed + +28 +00:02:13,020 --> 00:02:17,460 +antibodies يعني أجسام مضادة مجهزة في الخارج قاطعة + +29 +00:02:17,460 --> 00:02:22,040 +للمريض لحمايته من مرض معين أو يبادله من مرض معين + +30 +00:02:23,030 --> 00:02:26,950 +رقم تلاتة ليلة غريبة active and passive at the + +31 +00:02:26,950 --> 00:02:31,610 +same time بنعطي vaccine وبنعطي immunoglobulin وفي + +32 +00:02:31,610 --> 00:02:35,490 +مثلان الصحيح في الطب مشهورين جدا في هذا المجال + +33 +00:02:35,490 --> 00:02:41,670 +نبدأ فيهم انت منهم تلعب تيتا نص؟ تيتا نص و ال + +34 +00:02:41,670 --> 00:02:44,710 +rabies ال rabies يمكن يكون أسهل للشرح خلنا نشرحها + +35 +00:02:44,710 --> 00:02:49,120 +ال rabies ال rabies هو يبقى عبارة عن دا الكلاباللي + +36 +00:02:49,120 --> 00:02:53,980 +لو عض .. كلمة عض يعني .. لأنه طريقة الانتقال عن + +37 +00:02:53,980 --> 00:02:57,560 +طريق الكلاب اتضالة و الغالب اللي هم صورة فبعض + +38 +00:02:57,560 --> 00:03:01,020 +البلدان لإن الكلاب تم تطيمها و خلصنا من الكلاب + +39 +00:03:01,020 --> 00:03:04,040 +اتضالة فصار الانتقال عن طريق أشياء أخرى زي + +40 +00:03:04,040 --> 00:03:07,380 +الخفافيش على سبيل المثال و عن طريق السناجب .. + +41 +00:03:07,380 --> 00:03:09,720 +بتعرف السناجب الحلوين هدولة؟ هدولة اللي ممكن + +42 +00:03:09,720 --> 00:03:13,380 +تنقلونها ال .. في حيوانات كثيرة ممكن تنقل اللي هو + +43 +00:03:13,380 --> 00:03:18,520 +ال rapistال animal بيسموه rabid animal، rabid + +44 +00:03:18,520 --> 00:03:22,900 +animal يعني حيوان عنده مرض اللي هو rabies، rabid + +45 +00:03:22,900 --> 00:03:28,480 +animal ال rabid animal لو عرض إنسان، فالأن في + +46 +00:03:28,480 --> 00:03:33,200 +احتمالية ان هذا الشخص صار .. لأن هو exposed، صار + +47 +00:03:33,200 --> 00:03:37,680 +ايش انه exposed؟ تعرض، فالحاجة الأحسن اللي احنا + +48 +00:03:37,680 --> 00:03:41,380 +نعطيه، نعطيه vaccine و نعطيه antibodies، هو تعرض + +49 +00:03:41,380 --> 00:03:46,830 +alreadyمظبوط نعطيها antibodies لكن لإنه .. لإنه ال + +50 +00:03:46,830 --> 00:03:51,470 +virus اللي هو رحلة من الجلد إلى ال central nervous + +51 +00:03:51,470 --> 00:03:55,610 +system بتاخد أيام هذه الفترة الزمنية .. هذه الفترة + +52 +00:03:55,610 --> 00:04:02,190 +الزمنية كافية إنه أنا أشتغل بالطريقتينبقى احكم + +53 +00:04:02,190 --> 00:04:07,070 +المكان المصاب ب immunoglobulins immunoglobulins هل + +54 +00:04:07,070 --> 00:04:10,930 +بيقبل neutralization؟ لأ اللي هو ال anti .. ال + +55 +00:04:10,930 --> 00:04:17,190 +vaccine .. ال virus .. ال virus نفسه و على الجهة + +56 +00:04:17,190 --> 00:04:21,930 +التانية مثلا اللي هو العضة في رجله الشمال تمام؟ + +57 +00:04:21,930 --> 00:04:25,680 +بروح على أيديه اليمين و بعطيه vaccineليش بعضهم مش + +58 +00:04:25,680 --> 00:04:28,800 +في نفس المكان؟ عشان ال vaccine إيش هو؟ Antigen + +59 +00:04:28,800 --> 00:04:31,580 +وهذا اللي حطيناها بالأول Antibody، لو التقل + +60 +00:04:31,580 --> 00:04:34,100 +Antibody مع ال Antigen هي Value Neutralization، صح + +61 +00:04:34,100 --> 00:04:37,580 +ولا لأ؟ فبعطي ال vaccine في جهة و بعطي ال + +62 +00:04:37,580 --> 00:04:40,520 +immunoglobulin في جهة تانية كمان، ليش عطينا + +63 +00:04:40,520 --> 00:04:44,480 +vaccine في هذه الحالة؟ لإن في فترة زمنية طويلة ما + +64 +00:04:44,480 --> 00:04:48,680 +بين ال site of entry لإنه يصل ل center of impulse + +65 +00:04:48,680 --> 00:04:52,960 +system ففي فرصة إن الاسم يكون أسام مضادةالى هاي + +66 +00:04:52,960 --> 00:04:55,280 +مثلها ال active passive immunization في نفس اللي + +67 +00:04:55,280 --> 00:04:58,160 +هو الوقت لكن في غير بالأمر ايه هتعطيه منه بيبولين + +68 +00:04:58,160 --> 00:05:03,500 +ايه هتعطيه فاكسينه ده على حساب الحلالـ Active + +69 +00:05:03,500 --> 00:05:06,780 +Immunity ممكن تقسم الفاكسين إلى تلات أنواع أو تلات + +70 +00:05:06,780 --> 00:05:09,720 +أصحاب من الفاكسين الصندوق الأول الـ capsular + +71 +00:05:09,720 --> 00:05:12,580 +polysaccharide فستجدوا حالكوا بتاعه فوق كويس + +72 +00:05:12,580 --> 00:05:16,000 +Inactivated Protein Toxins اللي هي الـ Toxites + +73 +00:05:16,000 --> 00:05:20,540 +وبتاعه فوق وضبط كويس تلاتة killed bacteria or live + +74 +00:05:20,540 --> 00:05:23,540 +attenuated bacteria كلمة live يعني + +75 +00:05:29,820 --> 00:05:34,280 +بالنسبة للمعالجات المطلوبة، لكن البكتيريا اتعرضت + +76 +00:05:34,280 --> 00:05:38,340 +له معالجات جعلتها غير قادرة على إحداث طعام + +77 +00:05:38,340 --> 00:05:41,620 +الgenesis وبالتالي هي attenuated قرّى لزمى ال + +78 +00:05:41,620 --> 00:05:44,820 +infection، لكن غير قادرة على إتحاد ال disease + +79 +00:05:44,820 --> 00:05:50,810 +وبالتالي المريض بيحتك بمجموعة من البكتيرياو + +80 +00:05:50,810 --> 00:05:55,490 +بيقتلها معناه حاكيش انه عنده experience، كلمة + +81 +00:05:55,490 --> 00:05:58,710 +experience يعني عنده memory و عنده antibodies لو + +82 +00:05:58,710 --> 00:06:01,790 +البكتيريا يخلق مرة أخرى بطريقة natural، بيقدر + +83 +00:06:01,790 --> 00:06:04,570 +يقاوم، إذن captured by polysaccharide، inactivated + +84 +00:06:04,570 --> 00:06:08,790 +proteins تكسوك، تلقى a killed bacteria or live + +85 +00:06:08,790 --> 00:06:16,060 +attenuated bacteriaنجوم الـ meningitis فاكرينهم؟ + +86 +00:06:16,060 --> 00:06:19,620 +هم نفسهم النجوم اللي يلهم الكابسول صح ولا لا؟ + +87 +00:06:19,620 --> 00:06:23,540 +وبالتالي كون الكابسول is the major the main + +88 +00:06:23,540 --> 00:06:26,660 +violence factor of this organism يبقى المتوقع ان + +89 +00:06:26,660 --> 00:06:30,960 +ال vaccine هو هيستهدف أيضا اللي هو الكابسول فأول + +90 +00:06:30,960 --> 00:06:35,520 +واحدة استبتت كوكاس ميمونيا vaccine وأول صحية ينتمي + +91 +00:06:35,520 --> 00:06:39,400 +إلى صنفين في السوق موجود منه vaccines واحد بحتوى + +92 +00:06:39,400 --> 00:06:43,880 +على 23 serotypesفاكريني الوقت قبل ما اشرفتش؟ تلاتة + +93 +00:06:43,880 --> 00:06:46,640 +و تمانين؟ اربعة و تمانين اتكلمت عنها على طول خلاص + +94 +00:06:46,640 --> 00:06:50,680 +اربعة و تمانين .. اربعة و تمانين stereotypes بس + +95 +00:06:50,680 --> 00:06:54,160 +احنا لازم نجد ايش منهم؟ تلاتة و عشرين هذا ايش + +96 +00:06:54,160 --> 00:06:57,740 +واصفه تلاتة و عشرين؟ the most common the most + +97 +00:06:57,740 --> 00:07:01,900 +prevalent الاكتر شيوعا في المنطق ده مين اللي + +98 +00:07:01,900 --> 00:07:06,020 +بيعطوا هدوله؟ بيعطوا لناس اللي فوق الستين سنة + +99 +00:07:06,020 --> 00:07:11,450 +تمام؟ اللي هم normal ماعنفش اي مشكلةللي أعمارهم + +100 +00:07:11,450 --> 00:07:14,990 +أقل من ستين بس بيمتلكوا واحد أو أكتر من هذه الشروط + +101 +00:07:14,990 --> 00:07:20,730 +اللي احنا راح نتكلم عنها مثلا عنده diabetes، عنده + +102 +00:07:20,730 --> 00:07:25,110 +serosis، تلايف، عنده compromised spleen function، + +103 +00:07:25,110 --> 00:07:27,810 +يعني عنده مشكلة في وظيفة الـspleen، أو عنده + +104 +00:07:27,810 --> 00:07:31,350 +sphenectomy، يعني الـspleen تم استئثاره، تم + +105 +00:07:31,350 --> 00:07:35,390 +استئثاره وبطوحاه، تم استئثاره، هدول الناس لأ، ايه + +106 +00:07:35,390 --> 00:07:39,590 +ربما هم بياخدوا اللي هو ال vaccineالنوع التاني من + +107 +00:07:39,590 --> 00:07:43,530 +ال vaccines بيحتوي على عدد أقل بيحتوي على عدد أقل + +108 +00:07:43,530 --> 00:07:48,610 +وهم سبعة بس خدوا باركم it is coupled with a + +109 +00:07:48,610 --> 00:07:50,790 +carrier protein ايش يعني coupled with a carrier + +110 +00:07:50,790 --> 00:07:53,770 +protein؟ انا بقول دالما انه + +111 +00:07:56,090 --> 00:08:00,850 +لو هذا ال antigen هو ال protein بالمناسبة او ال + +112 +00:08:00,850 --> 00:08:02,770 +polysaccharide و ال polysaccharide او ال protein + +113 +00:08:02,770 --> 00:08:07,090 +they are antigenic لكن لأن الحجم matters يعني بيهم + +114 +00:08:07,090 --> 00:08:10,850 +الحجم في ال antigenات زي ما تتدرسوا في المناعة + +115 +00:08:10,850 --> 00:08:15,410 +ابتراحوا ان لو ركّبت انا هذا على carrier protein + +116 +00:08:15,410 --> 00:08:19,950 +بصير حجمه اكبر صحيح هذا مابش اي قيمة من ناحية ال + +117 +00:08:19,950 --> 00:08:24,210 +antigenات لكن القيمة لمين؟بالانتجين نفسه، لكن + +118 +00:08:24,210 --> 00:08:29,170 +كموليكيل كله كبر، فصوت ال immunogenicity بعته أعلى + +119 +00:08:29,170 --> 00:08:32,950 +وهذه عملية معروفة ومشهورة بنسمي المركب إما + +120 +00:08:32,950 --> 00:08:38,010 +conjugate أو adjuvant اللي هو مادة محفزة على ال + +121 +00:08:38,010 --> 00:08:41,110 +antigenicity نفهم الحالة على فكرة نستخدم ال + +122 +00:08:41,110 --> 00:08:44,730 +diphtheria toxoid كadjuvant أو كconjugate يعني + +123 +00:08:44,730 --> 00:08:49,130 +بنركب المركب اللي بنيها رام عالمين على diphtheria + +124 +00:08:49,130 --> 00:08:53,580 +toxoidيبقى الأولاني ال conjugated والثاني + +125 +00:08:53,580 --> 00:08:58,320 +conjugated أكم واحد الأولاني بيحتوي ايه؟ التاني + +126 +00:08:58,320 --> 00:09:05,860 +لمن يبقى في التاني لأن أطفال اللي لم يستجيبوا بشكل + +127 +00:09:05,860 --> 00:09:10,800 +جيد لل vaccine الأولاني يبقى بتوقع أن ال vaccine + +128 +00:09:10,800 --> 00:09:15,740 +التاني الاستجابة اللي هو أفضل ليش أفضل؟ لأنه كما + +129 +00:09:15,740 --> 00:09:19,340 +حكينا conjugated with diphtheria + +130 +00:09:23,030 --> 00:09:27,230 +في مصطلح بنا يستخدمه الآن هنفهمه كويس قدر نركز مع + +131 +00:09:27,230 --> 00:09:33,110 +بعض و بدون كتاب المراجع انتوا اربعة و تمانين اربعة + +132 +00:09:33,110 --> 00:09:37,170 +و تمانين لما انا ماسألش او ماعطيش محاضرة او + +133 +00:09:37,170 --> 00:09:43,010 +مااهتمش الا بتلاتة و عشرين تمام؟ او بلاش بتلاتة و + +134 +00:09:43,010 --> 00:09:47,830 +عشرين صار مضمونين مين اللي مش مضمونين؟ المبنيين + +135 +00:09:48,430 --> 00:09:53,470 +بطلنا نهتم بالـ23 فبقعدنا نهتمنا فيهم يردبوا و + +136 +00:09:53,470 --> 00:09:58,830 +يدوبوا و يخلصوا من المجتمع لكن الباقي موجودين + +137 +00:09:58,830 --> 00:10:03,290 +صحيح؟ يعني اللي لم نقتلهم بال vaccine وبالتالي + +138 +00:10:03,290 --> 00:10:07,470 +بنخلق من ما يسمى بـ Serotype Replacement ان اللي + +139 +00:10:07,470 --> 00:10:12,270 +مااهتملاش فيهم بال vaccine يحلوا محل اللي اهتمنا + +140 +00:10:12,270 --> 00:10:16,330 +فيهم بال vaccine واضحة ممكن و مش واضحة ان اوضحها + +141 +00:10:16,330 --> 00:10:21,850 +كمان مرةعندي 23 نوع او سلالة من البكتيريا كوضع لهم + +142 +00:10:21,850 --> 00:10:24,850 +فاكسين هدولة هارفوا البلد كلهم مالوجلهم مش سوء او + +143 +00:10:24,850 --> 00:10:29,410 +مكان يقعدوا فيه شهر perfection صح؟ ايش مصيرهم؟ الى + +144 +00:10:29,410 --> 00:10:36,130 +زوالة مين اللي بيضاروا؟ الباجين اللي مالهمش + +145 +00:10:36,130 --> 00:10:40,970 +antivirus في أجسامنا ممكن اي من هؤلاء يحلوا محل + +146 +00:10:40,970 --> 00:10:45,430 +الواحد من التلاتة وعشرين بظاهرة نسميها Serotype + +147 +00:10:45,430 --> 00:10:48,750 +Replacement نطمنكمفي السطر الأخير أو الليلة + +148 +00:10:48,750 --> 00:10:53,870 +الأخيرة من اللوحة بتقول التالت إن هذا التخوف اللي + +149 +00:10:53,870 --> 00:10:58,070 +هو Zero Type Replacement هو تخوف نظري ولم نجد أي + +150 +00:10:58,070 --> 00:11:02,670 +بيانات لحد الآن تدعم هذا التخوف النظري لكن هي + +151 +00:11:02,670 --> 00:11:08,010 +ظاهرة منطقية .. منطقية متوقعة وما زالت to be + +152 +00:11:08,010 --> 00:11:11,870 +evaluated يعني باستمرار الأرواح ده بتطلعوا على هل + +153 +00:11:11,870 --> 00:11:16,050 +صار انتشار لسوريات جديدة غير ال 23 أو غير السبعة + +154 +00:11:16,050 --> 00:11:19,040 +اللي احنا استخدمناهم أو لالكن لحد ما مافيش بيانات + +155 +00:11:19,040 --> 00:11:25,140 +تشير إلى انه ظاهرة مهمة و بتاعة نعترف من ال + +156 +00:11:25,140 --> 00:11:28,660 +capsular polysaccharide neisseria meningitidis + +157 +00:11:29,610 --> 00:11:35,310 +ناسيريا meningitis احنا اتكلمنا عنها و كنا بتعمل + +158 +00:11:35,310 --> 00:11:38,570 +explosive .. explosive يعني مفجري .. explosive + +159 +00:11:38,570 --> 00:11:45,130 +epidemics و ذكرنا بعض النقاط الحساسة اللي وين ممكن + +160 +00:11:45,130 --> 00:11:49,250 +تنتشر فيها من ضمن اننا كنا college students طلاب + +161 +00:11:49,250 --> 00:11:53,830 +الجامعة اللي بيعيشوا في سكن داخلي و المجننين الجدر + +162 +00:11:53,830 --> 00:11:59,340 +و هتلاحظوا ان اكتر فئات لابنقصح إننا نعطيهم ال + +163 +00:11:59,340 --> 00:12:02,340 +vaccine، هم الفئتين اللي ذكرتهم الآن، احنا كمسلمين + +164 +00:12:02,340 --> 00:12:06,520 +لخاصية جمهنين .. مجتمع الو السودان .. مجتمع الو + +165 +00:12:06,520 --> 00:12:12,200 +السودان و احنا كمسلمين النيزة ليه الحج، إنه قبل + +166 +00:12:12,200 --> 00:12:16,750 +الحج بناخد هذا أيضا ال vaccineاللي بيحتوي + +167 +00:12:16,750 --> 00:12:20,310 +الفاكسينة على أربع cellotypes من الكبسول ولازم + +168 +00:12:20,310 --> 00:12:28,950 +نحفظهم الآن ACW سهلين ACW 139 والأخيرة Y ACW 135 + +169 +00:12:28,950 --> 00:12:32,330 +اللي هو Y هلأ الأربعة اللي موجودة برضه ليه منه + +170 +00:12:32,330 --> 00:12:38,310 +شكلين شكل الأول conjugated والتاني non conjugated + +171 +00:12:38,310 --> 00:12:39,570 +لإن اللي بيحتوي على ال + +172 +00:12:44,820 --> 00:12:47,460 +it is given when there is a high risk of + +173 +00:12:47,460 --> 00:12:51,280 +meningitis when + +174 +00:12:51,280 --> 00:12:56,900 +students enter college and are living in dormitory + +175 +00:12:56,900 --> 00:13:01,640 +when military recruits enter boot camp اللي هو + +176 +00:13:01,640 --> 00:13:05,000 +المعسكرات التدريب والتجنيد or for travelers + +177 +00:13:06,390 --> 00:13:08,590 +المسافرين اللي احنا من ضمنهم الحجار اللي بيبقى + +178 +00:13:08,590 --> 00:13:12,310 +مسافرين to areas where meningitis الان في مصطلح + +179 +00:13:12,310 --> 00:13:16,930 +اول مرضاه هنا المحاضر الماضي مر او اللي جابنا كلمة + +180 +00:13:16,930 --> 00:13:21,750 +endemic endemic ميزنها عن epidemic المرض هو انه + +181 +00:13:21,750 --> 00:13:25,950 +hyper endemic wherein a disease incidence is + +182 +00:13:25,950 --> 00:13:30,790 +constantly high in one area يعني نسبة حدوث المرض + +183 +00:13:30,790 --> 00:13:32,210 +عالية في + +184 +00:13:35,630 --> 00:13:40,690 +نقول نسبة الإصابة سنوية مثلا 20 ألف إصابة لكل 100 + +185 +00:13:40,690 --> 00:13:44,450 +ألف شخص هذه نسبة عالية جدا لكن سنويا بتحدث هذي + +186 +00:13:44,450 --> 00:13:49,150 +بيسموها hyper endemic hyper endemic يعني مستوطن + +187 +00:13:49,150 --> 00:13:53,490 +المرض و يحدث بنسبة عالية النوع التالت بره من نجوم + +188 +00:13:53,490 --> 00:13:58,490 +ال meningitis Hemophilus influenza و من ميزاتها ان + +189 +00:13:58,490 --> 00:14:02,890 +ال capsule تبعتها is poorly immunogenic + +190 +00:14:05,990 --> 00:14:11,930 +لو حطيناها لحالها عند الإنسان للأسف بيكونش الإنسان + +191 +00:14:11,930 --> 00:14:15,970 +إلها أجسام مضادة بكميات كبيرة، لذا مافيش خيار + +192 +00:14:15,970 --> 00:14:20,010 +قدامها لأنه يكون conjugated و unconjugated، لازم + +193 +00:14:20,010 --> 00:14:25,310 +يكون مفلوس influenza vaccine contains the type B + +194 +00:14:25,310 --> 00:14:29,790 +polysaccharideالـ type B polysaccharide يبقى فيه + +195 +00:14:29,790 --> 00:14:34,210 +لنا strand واحد conjugated to diphtheria toxoid or + +196 +00:14:34,210 --> 00:14:39,130 +other carrier proteins يبقى فيه لنا conjugation + +197 +00:14:39,130 --> 00:14:42,510 +لإما diphtheria اللي هي اللي conjugated أو other + +198 +00:14:42,510 --> 00:14:46,130 +proteins it is given to children between the ages + +199 +00:14:46,130 --> 00:14:47,950 +of two to fifteen months + +200 +00:14:51,880 --> 00:14:56,700 +و قلتلكوا إن العمر حتى جدول ال vaccines في العالم + +201 +00:14:56,700 --> 00:15:02,180 +بتغير كل ما بتتراكم لنا بيانات جديدة بتحسن من + +202 +00:15:02,180 --> 00:15:05,820 +فهمنا لبعض الأمور ممكن نغيّر في جناح ال + +203 +00:15:05,820 --> 00:15:12,480 +vaccination فلما أنا أقول البداية من شهرين لحد 15 + +204 +00:15:12,480 --> 00:15:15,380 +شهر يمكن هذه الفترة اللي بتسبق + +205 +00:15:17,970 --> 00:15:21,990 +الزمن الملائم للبكتيريا ان تحدث فيه أمراض يعني + +206 +00:15:21,990 --> 00:15:25,870 +أكتر ناس بيصاروا بالأمراض هم الأطفال اللي فيها + +207 +00:15:25,870 --> 00:15:30,170 +السن و بعدها بشوية انها تابتنها قبل ما تبدأ هذه + +208 +00:15:30,170 --> 00:15:36,870 +المرحلة الخطرة بنعطي ال vaccine زي ما حكينا قبل + +209 +00:15:36,870 --> 00:15:39,590 +لحظات انها poorly immunogenic و it should be + +210 +00:15:39,590 --> 00:15:42,810 +coupled to بقولنا كده protein to enhance + +211 +00:15:42,810 --> 00:15:44,150 +immunogenicity + +212 +00:15:46,100 --> 00:15:51,120 +الان احنا بنعرف فاكسين اسمه DPT صح؟ DPT ثلاثي، + +213 +00:15:51,120 --> 00:15:54,680 +Diphtheria Pertussis وTetanus، فهي فاكسين الان + +214 +00:15:54,680 --> 00:16:01,240 +رباعي في الأسواق، بيشمل التلاتة بالاضافة إلى ال + +215 +00:16:01,240 --> 00:16:06,040 +Hemophilus influenza type B فصار رباعي، صار عشانها + +216 +00:16:06,040 --> 00:16:11,450 +فاكسين رباعيخلصنا .. خدوا بالكوا دقا .. خلصنا + +217 +00:16:11,450 --> 00:16:16,250 +المعلومات المتعلقة بتلت ميكروبيرات Spetacoccus + +218 +00:16:16,250 --> 00:16:20,410 +pneumonia, Muflis influenza و Nacinamide bites + +219 +00:16:20,410 --> 00:16:27,020 +اللي بتكلم فيه مقصود عن البكتيرا الرقم تلتبتعرفوا + +220 +00:16:27,020 --> 00:16:30,140 +السيرمونيلا التعيفى؟ السيرمونيلا التعيفى إلها + +221 +00:16:30,140 --> 00:16:33,520 +vaccine و لو رجعتوا للمحاضرة تبعتنا هنقولوا ال + +222 +00:16:33,520 --> 00:16:37,260 +prevention by vaccine طبعا الآن ال vaccine طبعا + +223 +00:16:37,260 --> 00:16:41,420 +السيرمونيلا التعيفى في منه صينفين واحد بيستهدف + +224 +00:16:41,420 --> 00:16:46,480 +الكابسول فنحطه مع مين؟ مع الكابسول، مع دولة واحد + +225 +00:16:46,480 --> 00:16:50,300 +لكل البكتيريا كل البكتيريا اللي هي killed bacteria + +226 +00:16:50,300 --> 00:16:55,620 +killed bacteria يعني السيرمونيلا ممكن نكون واحد أو + +227 +00:16:55,620 --> 00:16:56,180 +ندمجها + +228 +00:17:02,020 --> 00:17:07,740 +طبعا تسمونه طيفي vaccine is indicated يوصف ليه أو + +229 +00:17:07,740 --> 00:17:12,820 +يوصى بيه لمين to people who are traveling to areas + +230 +00:17:12,820 --> 00:17:17,620 +where typhoid fever is common يعني الحمض تيفؤيد + +231 +00:17:17,620 --> 00:17:24,240 +كان موجود أو منتشر بشكل كبير أو لأشخاص who are in + +232 +00:17:24,240 --> 00:17:28,420 +contact with typhoid fever patients or carriers + +233 +00:17:29,860 --> 00:17:36,620 +عشان احنا عارفين انه الانتقال في كالقول و الاحتكاك + +234 +00:17:36,620 --> 00:17:41,080 +المباشر مع المرضى قد يعني انت عارفين given aseptic + +235 +00:17:41,080 --> 00:17:47,520 +technique لو راح تخيلها عملية معقدة جداو مهما ببنى + +236 +00:17:47,520 --> 00:17:50,540 +من جهد .. مهما ببنى من جهد في الacidic technique + +237 +00:17:50,540 --> 00:17:55,300 +إلا يصير فيه breach خلل معين وبالتالي قد ينتقل + +238 +00:17:55,300 --> 00:17:59,960 +المرض فلما أنا أكون بتعامل مع مرضى و أنا at high + +239 +00:17:59,960 --> 00:18:05,590 +risk لما ممكن أاخد ايه؟ vaccine في هذه الحلقةالنوع + +240 +00:18:05,590 --> 00:18:07,710 +التالت من ال vaccine دو اللي عنه هو ال toxoid + +241 +00:18:07,710 --> 00:18:11,090 +vaccine و ال toxoid زي ما حكينا هي عبارة عن toxin + +242 +00:18:11,090 --> 00:18:15,010 +that has been modified كلمة modified قد تكون ال + +243 +00:18:15,010 --> 00:18:19,170 +modification chemical قد تكون physical قد تكون + +244 +00:18:19,170 --> 00:18:22,050 +genetic engineering يعني ممكن نعمل modification + +245 +00:18:22,050 --> 00:18:27,750 +بالهندسة الوراقية ناخد أول مثال Cornebacterium + +246 +00:18:27,750 --> 00:18:32,870 +diphtheria vaccine حد سمع منكوا أن فلان أصيب بال + +247 +00:18:32,870 --> 00:18:37,400 +diphtheriaمافيش، ليش مافيش؟ لأن الطلاب العربية + +248 +00:18:37,400 --> 00:18:41,320 +indicated يعني immunization against diphtheria is + +249 +00:18:41,320 --> 00:18:44,940 +indicated for every child، يعني حضراتكم لما كنتوا + +250 +00:18:44,940 --> 00:18:49,520 +child، كنتوا children، أخدتها للفاكسينكل العالم + +251 +00:18:49,520 --> 00:18:54,860 +اللي بيصلوا .. اللي بيفططوا الحضارة بياخد الـDPT + +252 +00:18:54,860 --> 00:18:59,100 +كنزه من الفكس، لذلك نسبة الدفتيريا تقريبا .. مش + +253 +00:18:59,100 --> 00:19:03,540 +تقريبا في غزة، إلها من سنوات صفر، إلها من سنوات + +254 +00:19:03,540 --> 00:19:10,560 +صفر، يعني الـToxoid لحاله كان كفيل بإنه يحمينا من + +255 +00:19:10,560 --> 00:19:13,240 +ال .. ال .. ال .. ال .. هذا .. هذا نقطة من فكرة + +256 +00:19:24,760 --> 00:19:28,480 +أنا بحكي ك تكسويد مش ك fragment من البكتيريا أخرى، + +257 +00:19:28,480 --> 00:19:30,840 +بس لأه .. لإن هو أصلا راح نكون نستخدمها ككاريا، + +258 +00:19:30,840 --> 00:19:34,840 +فهي كتيريا كبيرة، هو كبير، فبتاعي immunogenic جيد، + +259 +00:19:34,840 --> 00:19:39,700 +immunogenic جيد، أنا فهمت سؤالك لأه متاخرة، إنه .. + +260 +00:19:39,700 --> 00:19:43,680 +هو أنا ب .. ب .. بتكلم على ال fragment، إنه أنا + +261 +00:19:43,680 --> 00:19:48,020 +خدت تكسويد لحاله، ماخدتش معاه مراكبات أخرى كبسول + +262 +00:19:48,020 --> 00:19:50,340 +ولا خدت معاه أنتجلات تانية + +263 +00:19:54,090 --> 00:19:57,070 +كده المرة عرفنا انه بيعطل منين؟ for every child + +264 +00:19:57,070 --> 00:20:02,010 +على تلت جرعات اتنين اربع اشتهور من الامر و بعد سنة + +265 +00:20:02,010 --> 00:20:05,570 +من اخر جرعة يعني بيسموها booster dose اللي هي + +266 +00:20:05,570 --> 00:20:09,250 +الجرعة الداعمة او الجرعة التنشيطية اللي هي اسمها + +267 +00:20:09,250 --> 00:20:13,710 +باللغة العربية اما داعمة وهي الصحيحة الترجمة + +268 +00:20:13,710 --> 00:20:17,030 +الحرفية هي ال boost معناها دعم فbooster dose لكن + +269 +00:20:17,030 --> 00:20:21,190 +احنا برضه طبيا بيطلق على الجرعة التنشيطية اللي + +270 +00:20:21,190 --> 00:20:27,490 +بتذكر جهاز المناعرقم اتنان طبعا لحظه الأولاني اللي + +271 +00:20:27,490 --> 00:20:31,810 +هو في الجوجل الأولى it is formaldehyde treated ايش + +272 +00:20:31,810 --> 00:20:35,730 +يعني formaldehyde treated يعني اولج بمادة اللي هو + +273 +00:20:35,730 --> 00:20:39,990 +ال formaldehyde رقم اتنان Clostridium Tetani او + +274 +00:20:39,990 --> 00:20:43,150 +Clostridium Tetani vaccine contains tetanus toxoid + +275 +00:20:43,150 --> 00:20:46,970 +and is given to everyone زي نفسي ما هو الدول + +276 +00:20:46,970 --> 00:20:52,160 +الفلافيةearly in life وممكن بعد فترة وكل فترة يعني + +277 +00:20:52,160 --> 00:20:54,820 +هو غالبا بيعطي protection لخمس سنوات لو أعطينا + +278 +00:20:54,820 --> 00:21:00,540 +كمان جوع على خمس سنوات فبينشط اللي لازم الان + +279 +00:21:00,540 --> 00:21:04,340 +لاحظوا الأول والتاني تكسويت تكسويت وكانوا بالفعل + +280 +00:21:04,340 --> 00:21:08,100 +تتنسع اننا تقريبا شبه zero بياخدوا شبه zero تيجي + +281 +00:21:08,100 --> 00:21:10,560 +للقه تلاتة اللي هي ال borderline laparotosis + +282 +00:21:10,560 --> 00:21:10,940 +vaccine + +283 +00:21:13,620 --> 00:21:17,240 +but includes other proteins as well، خدوا بالكم، + +284 +00:21:17,240 --> 00:21:23,700 +اللي في غزة DBT، و ال DBT هي Deuterium Pertosis + +285 +00:21:23,700 --> 00:21:27,980 +Tetanus والتلاتة دوكسول فقط لالي، we are protected + +286 +00:21:27,980 --> 00:21:32,040 +من ال vaccine هذا، يعني حمينا من ال Deuterium و + +287 +00:21:32,040 --> 00:21:35,260 +حمينا من ال Tetanus، لكن we are not totally + +288 +00:21:35,260 --> 00:21:41,020 +protected من اللي هو ال ال ال ال Pertosisو أكبر دي + +289 +00:21:41,020 --> 00:21:43,920 +عيبالك ان كل اللي عالم اللي بيستخدم هذا ال vaccine + +290 +00:21:43,920 --> 00:21:47,380 +برضه مش protected against this disease وكان في + +291 +00:21:47,380 --> 00:21:51,620 +الكيان المحتر للغاصب كان قبل عدة سنوات ممكن + +292 +00:21:51,620 --> 00:21:55,840 +خمسمائة حالة سجلوه من ال pertosis وان في غزة في + +293 +00:21:55,840 --> 00:22:00,140 +symptoms typical pertosis لكن الطبان يبدو انهم مش + +294 +00:22:00,140 --> 00:22:03,920 +مقتنعين ان في انا ذات بقى ال pertosisفطبعا هو + +295 +00:22:03,920 --> 00:22:08,260 +الpertosis self-limiting على الرغم من خطورته + +296 +00:22:08,260 --> 00:22:12,780 +وصعوبته إلا إنه حتى لو ما أعطينا علار غالبا + +297 +00:22:12,780 --> 00:22:16,120 +بالنسبالي لو كنت فيه ضعيفة جدا، قادرة جدا استاذ + +298 +00:22:16,120 --> 00:22:19,900 +بالي متعرف بينا، شو هو الpertosis؟ هو السعالة + +299 +00:22:19,900 --> 00:22:22,840 +الديكي، السعالة ديكي، بعمل كل شي أنا بسموه السعالة + +300 +00:22:22,840 --> 00:22:28,790 +الديكي مانتش كذا؟ال .. ال disease بيتكون .. عشان + +301 +00:22:28,790 --> 00:22:32,310 +ذلك لازم نشرح أولاد مولودة من فهم أعمق يا بنات فهم + +302 +00:22:32,310 --> 00:22:36,590 +أعمق للمتباعة ال genesis أو لأي مسألة في الدنيا + +303 +00:22:36,590 --> 00:22:41,330 +بيقدر يتخذ قرار أفضل و أنا دايما بقول مثال بقول + +304 +00:22:41,330 --> 00:22:48,210 +أنه الشخص في المنزل قد يعني يتعرض لقرار يعتبره + +305 +00:22:48,210 --> 00:22:54,870 +ظالم من رب الأسرة من الوالد يعتبره ظالم و غير راضي + +306 +00:22:54,870 --> 00:22:59,160 +عن هذا القرارو انا بقول انه لأ يعني لو انت عرفت + +307 +00:22:59,160 --> 00:23:03,560 +البعلومات اللي عند رب الأسرة ربما اتخذت نفس القرار + +308 +00:23:03,560 --> 00:23:07,480 +و غالبا .. ده بت .. ممكن بتلاحظوا ال .. الامهات + +309 +00:23:07,480 --> 00:23:13,410 +بتعلم البنات و بيقولهم غلط و بيقولهم صح تمام؟طبعا + +310 +00:23:13,410 --> 00:23:17,230 +لو رجعنا بالتاريخ ما يلقوم كافية من أية بنت كانت + +311 +00:23:17,230 --> 00:23:20,170 +أما تقولها نفس الكلام اللي هي بتقولكواياه و هي ما + +312 +00:23:20,170 --> 00:23:22,970 +تسمعش الكلام و هي ماكانتش يسمع الكلام يعني سلسلة + +313 +00:23:22,970 --> 00:23:26,730 +من إيه لكن اللي أنا بوصلهياه أنه من يمتلك + +314 +00:23:26,730 --> 00:23:30,850 +المعلومات يمتلك المعلومات في شكل عام مش ضرور فيه + +315 +00:23:30,850 --> 00:23:32,970 +دراسة يعني و عندها كل المعلومات موجودة لكن بتاخد + +316 +00:23:32,970 --> 00:23:37,320 +قرار خاطرلكن هم اللي بيمتلك معلومات كثيرة غالبا + +317 +00:23:37,320 --> 00:23:42,880 +بياخد قراره مبني على قاعد أوسع من المعرفة فبالتالي + +318 +00:23:42,880 --> 00:23:48,680 +بيكون أفضل، ليش بنحك الكلام هذا؟ بنحك الكلام هذا + +319 +00:23:48,680 --> 00:23:52,880 +جاب ال .. طلبات اتكلمت عن ال bacteroidis + +320 +00:24:02,710 --> 00:24:07,310 +أنا عندي نية إن أنا أقوم بمحاضرة عامة عن ال normal + +321 +00:24:07,310 --> 00:24:11,030 +flora و عن الخير اللي بيحصل منها، لحظة تخيلوا + +322 +00:24:11,030 --> 00:24:15,830 +فصائل الدم is modified by normal flora، diabetes، + +323 +00:24:15,830 --> 00:24:19,630 +بلد السكر قد ينطقه، فيه دراسات ومؤشرات تقول إن + +324 +00:24:19,630 --> 00:24:23,650 +normal flora لها علاقة بهلوته، و نسبة انتشاره + +325 +00:24:24,440 --> 00:24:28,300 +المزاج تبعنا، إذا كان كويس أو مش كويس، إليه علاقة + +326 +00:24:28,300 --> 00:24:32,240 +بالنظر في الورق، نعم، فيعني إذا كانت تلعب بقى + +327 +00:24:32,240 --> 00:24:36,700 +بميرتنا و بتغير من ال mood تبعتنا، فبتجرب اللي .. + +328 +00:24:36,700 --> 00:24:40,340 +اللي .. اللي .. اللي بيخليك كده من الكلام إن + +329 +00:24:40,340 --> 00:24:46,650 +الإنسانغالبا بي .. بيحاول يقطف ثمار المعرفة مبكرا، + +330 +00:24:46,650 --> 00:24:50,550 +بمعنى انه بس يعرف معرفة جديدة، بيحاول يستغلها، + +331 +00:24:50,550 --> 00:24:54,470 +فمثلا، عرفنا ان البنتين هي اللي بتسيب الأمراض، + +332 +00:24:54,470 --> 00:25:01,020 +kill them all، اقتلوها99% بتستخدمها في 99.9% اللي + +333 +00:25:01,020 --> 00:25:05,580 +بتقول تمام؟ طهر إيدك، طهر إجليك، أهمال أكتر، خلاء + +334 +00:25:05,580 --> 00:25:09,020 +انتباطيك، وفي الآخر نكتشف إنه هذه الإجراءات + +335 +00:25:09,020 --> 00:25:13,260 +بتسببنا عمرها، مصبوط أو مش مصبوط؟ طبعاً الخير .. + +336 +00:25:13,260 --> 00:25:17,500 +الخير في Noble Flora ناتج عن التغيرات اللي .. اللي + +337 +00:25:17,500 --> 00:25:19,940 +.. اللي الإنسان في الغدا و التغيرات في الأدوية و + +338 +00:25:19,940 --> 00:25:22,540 +في العلاجات و في .. في .. في ..وفي طريق ال life + +339 +00:25:22,540 --> 00:25:25,140 +style تبعه وانا بتقولكوا مرة مهتمة انا بتقولكوا + +340 +00:25:25,140 --> 00:25:29,940 +انه تغيير النظام اللي يده إلى الأبقار أدى .. ده + +341 +00:25:29,940 --> 00:25:33,060 +الأبقار .. أدى كده انه يصير جزء من النظام اللي هو + +342 +00:25:33,060 --> 00:25:39,380 +تلاتة equalized أو 15787يبقى فعلا من العميق + +343 +00:25:39,380 --> 00:25:47,220 +بيعطينا إمكانية التدخل بدون ما نقضي ال .. ال .. ال + +344 +00:25:47,220 --> 00:25:49,860 +.. ال .. ال .. البيئة و نقضي ال .. ال .. ال .. ال + +345 +00:25:49,860 --> 00:25:53,220 +.. الbalance اللي على مدار القرون موجود + +346 +00:25:53,220 --> 00:25:58,120 +البورميلابيكوس كنا فاكرين إن الـtoxoid أو الـtoxin + +347 +00:25:58,120 --> 00:26:02,220 +هو ال major phytonic component of the bacteria + +348 +00:26:05,100 --> 00:26:08,800 +في الدفتيرك كرابنا صحيح في التتروس كرابنا صحيح لكن + +349 +00:26:08,800 --> 00:26:13,020 +في كرابنا مش صحيح لإنه لجهر البكتيريا اللي ها هيم + +350 +00:26:13,020 --> 00:26:17,920 +أجلوتينين بيتصق في السيليا اللي موجودة في التراكيا + +351 +00:26:17,920 --> 00:26:21,100 +وبتنقل خلال ما زمينا قلنا في الحركة السيليا + +352 +00:26:21,100 --> 00:26:25,920 +reaction اللي هو أساس طرد السبيوتم اللي بيخرج من + +353 +00:26:25,920 --> 00:26:30,520 +رسم الإنسان وبالتالي الفاكسين الجديد بيحتوي على + +354 +00:26:31,260 --> 00:26:35,820 +بيحتوي على فلامنتاس هم أجلوتنين و البرتكتين و + +355 +00:26:35,820 --> 00:26:39,460 +دلوقتي المركبين اللي هم علاقة بالبعض في نفس + +356 +00:26:39,460 --> 00:26:43,340 +الثرائح البكية فالان ال vaccine اللي موجود في + +357 +00:26:43,340 --> 00:26:50,960 +أمريكا بيحتوي على التكسور بقى كما هو زائد اللي هو + +358 +00:26:50,960 --> 00:26:54,660 +ال فلامنتاس هم أجلوتنين زائد اللي هو البرتكتين + +359 +00:26:54,660 --> 00:26:56,840 +اللي هم ال components of the cell wall + +360 +00:27:00,320 --> 00:27:04,300 +هنبدأ ان شاء الله مشوار السيمنارات للطالبات، كل + +361 +00:27:04,300 --> 00:27:08,600 +طالبة هتقدم خمس دقائق موضوع ان شاء الله يعني جديد + +362 +00:27:08,600 --> 00:27:12,120 +في ال microbiology ليس بضرورة يكونوا علاقة في ال + +363 +00:27:12,120 --> 00:27:15,260 +medical microbiology، لكن على الأقل بوسع مداركنا + +364 +00:27:15,260 --> 00:27:19,080 +ومفاهيمنا وهنبدأ ان شاء الله اليوم مع نور الصفة + +365 +00:27:19,080 --> 00:27:24,320 +دي، أول presentation اللي هتحاول تقدمها خلال خمس + +366 +00:27:24,320 --> 00:27:24,380 +دقائق + +367 +00:27:29,230 --> 00:27:32,850 +أول اسم البكتيريا موجودة في كل مكان، موجودة في + +368 +00:27:32,850 --> 00:27:36,150 +أجسامنا، في النبات، في الحيوانة، في كل مكان، منها + +369 +00:27:36,150 --> 00:27:39,450 +ما هو مفيد، منه ما هو ضاد اليوم راح أحكيلكم عن + +370 +00:27:39,450 --> 00:27:42,610 +إحدى فوائد البكتيريا إن البكتيريا بتنتج لنا + +371 +00:27:42,610 --> 00:27:46,150 +إلكترونات عن طريق هذه الإلكترونات بتستطيع إنولد + +372 +00:27:46,150 --> 00:27:50,050 +كهربا، فسموها هذه البكتيريا fuel microorganism + +373 +00:27:50,050 --> 00:27:56,470 +buttery bacteriaهذه البكتيريا من .. من أنواع هذه + +374 +00:27:56,470 --> 00:27:59,190 +البكتيريا نسأل على هذه البكتيريا اللى هى بكتيريا + +375 +00:27:59,190 --> 00:28:02,830 +ال Rodiferrix ferradinaceae هذه البكتيريا هى من + +376 +00:28:02,830 --> 00:28:06,030 +عائلة ال common denisius هذه من ال genius + +377 +00:28:06,030 --> 00:28:10,590 +Rodiferrix species ferradinaceae هذه البكتيريا أول + +378 +00:28:10,590 --> 00:28:13,290 +مرة عزلوها من رواسف Vanguela + +379 +00:28:17,760 --> 00:28:21,760 +هذه البكتيريا إلها خصائص تخلّيها مختلفة عن باقي + +380 +00:28:21,760 --> 00:28:25,660 +البكتيريات، هادي أن أثناء عملية تنفسها تستخدم + +381 +00:28:25,660 --> 00:28:29,280 +الحديد في التنفس تبعها، و بتتغذى زي أي نوع من + +382 +00:28:29,280 --> 00:28:35,500 +أنواع البكتيريا على الجليكوز، سيليلوز، استكاروزهذه + +383 +00:28:35,500 --> 00:28:39,520 +البكتيريا انه زى ما قولت قبلها انه بتنتج إلكتروناط + +384 +00:28:39,520 --> 00:28:43,740 +عن طريقة تستطيع انها تولد كهربا من خلالها هلقيت + +385 +00:28:43,740 --> 00:28:48,520 +كيف هي راح تولد الكهربا؟ قولت انه هي بتستخدم أثناء + +386 +00:28:48,520 --> 00:28:52,880 +عملية التنفس الحالية و بتستخدم كمان الجليكوز كمصدر + +387 +00:28:52,880 --> 00:28:56,180 +للغذاء لها، بتروح عن طريقهم، بتكسلهم فبتنتج + +388 +00:28:56,180 --> 00:29:00,310 +إلكتروناط، هذه الإلكتروناطبتعطينا كهربا وبتفضل انه + +389 +00:29:00,310 --> 00:29:03,930 +تعمل هذه العملية عن درجة حرارة من 4 درجة سيليزيا ل + +390 +00:29:03,930 --> 00:29:07,830 +30 درجة سيليزيا ولكن ال optimum لها 25 درجة + +391 +00:29:07,830 --> 00:29:12,890 +سيليزيا كيف انه نكشف عنها هى مبدأ العمل سكر جليكوز + +392 +00:29:12,890 --> 00:29:17,410 +مع ميه بوجود الحديد رح يعطينا CO2 مع الحديد نفس + +393 +00:29:17,410 --> 00:29:20,910 +الشغل مع ايونات الهيدروجين وحيكون الالكترونات هي + +394 +00:29:20,910 --> 00:29:25,390 +عبارة عن اولى عشان نفهم مبدأ العمل رح اورجيكم + +395 +00:29:25,390 --> 00:29:25,790 +فيديو + +396 +00:29:29,330 --> 00:29:32,610 +أول شغلة أن النبات راح يعمل عمليات بناء الطوائف + +397 +00:29:32,610 --> 00:29:49,490 +راح ينتج من خلاله سكر ال jericots ال + +398 +00:29:49,490 --> 00:29:52,030 +macro organism نعتبرها اللي هي ال Rodoflax في ال + +399 +00:29:52,030 --> 00:29:56,030 +ردينيز راح تتغذى عليها فرح تنتجلنا ال CO2 و رح + +400 +00:29:56,030 --> 00:29:57,530 +تنتجلنا إلكترونات + +401 +00:30:08,420 --> 00:30:11,520 +هلقيت رح نحطها تعتبر ان احنا حطيناها في جهاز + +402 +00:30:11,520 --> 00:30:14,120 +بيحتوي على انود و على كاتوب و على ال membrane + +403 +00:30:14,120 --> 00:30:17,040 +المادة اللي جرافيك ال membrane المادة اللي جرافيك + +404 +00:30:17,040 --> 00:30:20,340 +وظيفته ان يسهل عملية نقل الألوانات الهاروجين من + +405 +00:30:20,340 --> 00:30:24,060 +ناحية الانود لناحية الكاتوب هلقيت الإلكترونات بما + +406 +00:30:24,060 --> 00:30:28,240 +انها positive charge رح .. negative charge رح + +407 +00:30:28,240 --> 00:30:32,700 +تنتقل عن الانود لمصباح الكهربائي عشان نشوف إذا هي + +408 +00:30:32,700 --> 00:30:34,600 +هتولد من الكهربا ولا مش هتولد + +409 +00:30:54,390 --> 00:30:57,810 +بما أن الضواط يعني أش تحتوى على إلكترونات و إنها + +410 +00:30:57,810 --> 00:31:06,110 +فعلا بتولد كهربا راح ترجع طبعا على ال cathode و + +411 +00:31:06,110 --> 00:31:09,930 +راح تربط ما بين ال oxygen و ال hydrogen اللي انتقل + +412 +00:31:09,930 --> 00:31:13,910 +من الأنوال لل cathode و بتنتج لنا من خلاله ميه هذه + +413 +00:31:13,910 --> 00:31:15,770 +المبدأ العملية بشكل مختصر + +414 +00:31:23,740 --> 00:31:26,720 +وهي شكل العملية مرة تانية أنه قانون .. إجت + +415 +00:31:26,720 --> 00:31:29,860 +البكتيريا أنتجتنا إلكينات من القانون لنصبح + +416 +00:31:29,860 --> 00:31:34,380 +الكهربائي للكيثود فانتجتنا بعدها ميه شو المستقبل + +417 +00:31:34,380 --> 00:31:37,300 +هذه البكتيريا؟ هذه البكتيريا لو إجلنا بدنا نقارنها + +418 +00:31:37,300 --> 00:31:40,920 +بينها و بين كمية الكهربا اللي بتنتج في العالم طبعا + +419 +00:31:40,920 --> 00:31:44,140 +و بين الشركات طبعا الكمية بالنسبة لها كمية قليلة + +420 +00:31:44,140 --> 00:31:48,600 +بس المش .. المهم أنه هي البكتيريا هاي بتساعد على + +421 +00:31:48,600 --> 00:31:54,040 +إنتاج الكهربابنتخيل شوية ان هذه الكهربا وصلت عنا + +422 +00:31:54,040 --> 00:31:57,300 +لغزة .. اه هذه البكتيريا وصلت عنا لغزة و احنا + +423 +00:31:57,300 --> 00:32:00,420 +املنا ان الكهربا بدل ما تشير تجينا 8 ساعات تشير + +424 +00:32:00,420 --> 00:32:04,300 +على الأقل تجينا 10 ساعات، 12 ساعة فهذه البكتيريا + +425 +00:32:04,300 --> 00:32:09,040 +وصلت عنا وقدرنا نستغلها بشكل صحيح ووفرنا الأجهزة و + +426 +00:32:09,040 --> 00:32:12,360 +الإمكانيات من خلالها فانتجتنا الكهرباو في نفس + +427 +00:32:12,360 --> 00:32:17,580 +الوقت في بكتيريا تانية اسمها Chuanella Undecinens + +428 +00:32:17,580 --> 00:32:21,360 +هذه البكتيريا برضه موجودة في مياه المجاري هذه + +429 +00:32:21,360 --> 00:32:25,300 +البكتيريا لو ايضا اقدرنا نعزلها بتشتغل بنفس مبدأ + +430 +00:32:25,300 --> 00:32:30,560 +العمل و بتستطيع انه تنتجنا كهربا فلو وصلت لغزة مرة + +431 +00:32:30,560 --> 00:32:34,700 +تانية و استغلناها بشكل صحيح هترجع مرة تانية لغزة + +432 +00:32:34,700 --> 00:32:40,470 +تكون موضوع شكرابسم الله الرحمن الرحيم اليوم + +433 +00:32:40,470 --> 00:32:43,930 +موضوعنا هيتحدث عن نوع من أنواع البكتيريا بعمل على + +434 +00:32:43,930 --> 00:32:50,550 +تحويل فصائل الدم المختلفة من a او b او ab إلى + +435 +00:32:50,550 --> 00:32:53,890 +الفصيلة o باستعمال نوعين من أنواع البكتيريا ال + +436 +00:32:53,890 --> 00:32:57,990 +bacterium fragilis and النوع التاني هو عبارة عن + +437 +00:32:57,990 --> 00:33:01,210 +الفلافوبكتيريا meningospectum ويلها اسم اخر وهي + +438 +00:33:01,210 --> 00:33:03,730 +عبارة او الاسم بتاعنا مشهورة فيه هي + +439 +00:33:03,730 --> 00:33:06,710 +elizabethkingia meningospectum هذا هو النوع الاول + +440 +00:33:06,710 --> 00:33:12,330 +من البكتيرياbacteria it's pressure loss هى الأنواع + +441 +00:33:12,330 --> 00:33:15,810 +او الخصائص اللى تتمتع بيها هذا النوع من البكتيريا + +442 +00:33:15,810 --> 00:33:19,810 +طبعا obligate aerobic واخدنا في مصابق عن معنى + +443 +00:33:19,810 --> 00:33:22,470 +obligate aerobic و قعدين ال gram negative بصلاي + +444 +00:33:22,470 --> 00:33:25,610 +وهى الصورة موضحالنا تحت ال microscope بعد ما عملنا + +445 +00:33:25,610 --> 00:33:28,990 +لها stain و بتأكدنا انها negative بصلاي و هي عبارة + +446 +00:33:28,990 --> 00:33:31,810 +عن normal flora موجودة in intestinal attract in + +447 +00:33:31,810 --> 00:33:36,270 +human كولونز بتكون في حالتها الطبيعيةبتبقى flora + +448 +00:33:36,270 --> 00:33:39,410 +microflora أو normal flora لكن في حالة ال abnormal + +449 +00:33:39,410 --> 00:33:42,570 +conditions فورا بتتحول الى infections بتسبب ال + +450 +00:33:42,570 --> 00:33:46,130 +endogenous infections طبعا هي غير متحركة و ليست من + +451 +00:33:46,130 --> 00:33:48,230 +أنواع ال endosporous لأن ال endosporous هي كانت + +452 +00:33:48,230 --> 00:33:51,210 +لها نوعين مشهورين، فيهم هي عبارة عن الفاسيغاس + +453 +00:33:51,210 --> 00:33:54,990 +والبنستريديا طبعا هي أيضا من أنواع البكتيرا + +454 +00:33:54,990 --> 00:33:58,990 +المقاومة للبنسلين حيث تمت على إنتاج لل beta + +455 +00:33:58,990 --> 00:34:04,220 +-lactamaseأيضًا نستطيع عزلها عن طريق نوع من أنواع + +456 +00:34:04,220 --> 00:34:07,140 +ال agar هو عبارة عن ال bacterium spill esculin + +457 +00:34:07,140 --> 00:34:11,020 +agar يرمز بالرمز BBA هذا النوع من البكتيريا بتروح + +458 +00:34:11,020 --> 00:34:16,600 +بتحولي أو بتحللي ال esculin بتحوله ل dextrose and + +459 +00:34:16,600 --> 00:34:20,580 +esculetin فبتروح تتفاعل هذه المكونات لنتجات مع + +460 +00:34:20,580 --> 00:34:24,980 +الفرق iodines أو الفرق ions هذا الفرق موجود بشكل + +461 +00:34:24,980 --> 00:34:28,300 +طبيعي في داخل هذا النوع من ال mediaتمام بعد هيك + +462 +00:34:28,300 --> 00:34:31,520 +بيروح بيتحول للوسط حوالين ال coolness من dark + +463 +00:34:31,520 --> 00:34:34,680 +brown إلى black color و طبعا هذا النوع من + +464 +00:34:34,680 --> 00:34:37,880 +البكتيريا يعاد selective media إلى ال isolation + +465 +00:34:37,880 --> 00:34:41,600 +and identification of this microbe هلا ال + +466 +00:34:41,600 --> 00:34:44,220 +pathogens هي اللي هتكوّنها، حكينا هي موجودة في شكل + +467 +00:34:44,220 --> 00:34:47,480 +طبيعي ال normal flora intestinal tract في حالة ال + +468 +00:34:47,480 --> 00:34:50,320 +abnormal conditions بتعملي pathogensهذه ال + +469 +00:34:50,320 --> 00:34:52,900 +pathogens ممكن تتطور و تصل الى أنحاء عديدة في + +470 +00:34:52,900 --> 00:34:56,800 +الجسم ممكن تصل ل cns ال cns هو عبارة عن ال central + +471 +00:34:56,800 --> 00:35:00,040 +nervous system و ممكن تصل ل ال head لنيك ل ال + +472 +00:35:00,040 --> 00:35:04,950 +tissues soft tissues ل ال skinالتيترا عندى طبعا هى + +473 +00:35:04,950 --> 00:35:08,590 +قادرة او هى اساسا من احد مكوناتها ال capsule ال + +474 +00:35:08,590 --> 00:35:11,610 +capsule تبعها يعاد polysaccharides هذا بده يحميها + +475 +00:35:11,610 --> 00:35:15,550 +من الفاجوسيتوسس انها تكون بمهاجمتها و كذلك بتعمل + +476 +00:35:15,550 --> 00:35:18,430 +على إنتاج ال abscess formations اللي هي الخراج + +477 +00:35:18,430 --> 00:35:24,030 +اضافة ان ذلك بتسبب للبكتيريا في حالة انها وصلت على + +478 +00:35:24,030 --> 00:35:29,250 +الدم بشكل غير طبيعى و ايضا بتسبب لألم فى البطن هى + +479 +00:35:29,250 --> 00:35:35,320 +الصورة المضحكة عنها و بتسبب لبعض من الدياريالل + +480 +00:35:35,320 --> 00:35:37,880 +treatment، العلاج من هذا النوع من البكتيريا، طبعا + +481 +00:35:37,880 --> 00:35:40,980 +عندي ال antimicrobial متروناغازولز and + +482 +00:35:40,980 --> 00:35:45,480 +كوليمفينيكول، بدي أعمل inhibitor لل produce اللي + +483 +00:35:45,480 --> 00:35:48,000 +هي بتنتجها اللي هي ال beta-lactamase أو ال beta + +484 +00:35:48,000 --> 00:35:52,460 +-lactam، لو روحنا جمعنا بين ال beta-lactamase + +485 +00:35:52,460 --> 00:35:55,980 +inhibitions and ال beta-lactamase antibiotic، بهذا + +486 +00:35:55,980 --> 00:35:59,900 +الطريقة بقدر أنا أمنع من إنتاج هذا النوع عشان ما + +487 +00:35:59,900 --> 00:36:02,760 +تسببليش ال infections الموجودةاللأ ال medical + +488 +00:36:02,760 --> 00:36:05,480 +research حكيمها بداية أنها بتعمل على تحويل فصائل + +489 +00:36:05,480 --> 00:36:11,320 +الدم المختلفة من A أو B أو AB إلى فصيلة O فبالتالي + +490 +00:36:11,320 --> 00:36:13,800 +ال medical راح نستعملها في ال blood طبعا هي + +491 +00:36:13,800 --> 00:36:16,440 +بتستعمل في مجالات تانية زي ال cancer لكن اختصارا + +492 +00:36:16,440 --> 00:36:18,920 +للمقتر هنتحدث فقط عن ال blood ال + +493 +00:36:18,920 --> 00:36:22,700 +bacteriophagelosis بتعملي على إنتاج أنزيم معين + +494 +00:36:22,700 --> 00:36:26,620 +بتقدر فيه تحويل فصيلة الدم بالتحديد الفصيلة O إلى + +495 +00:36:26,620 --> 00:36:32,740 +الفصيلة A الفصيلة B إلى الفصيلة Oهذه الطريقة بتكون + +496 +00:36:32,740 --> 00:36:36,260 +عن طريق تكسير لل antigenats اللى موجودة على ال + +497 +00:36:36,260 --> 00:36:39,980 +surface لل RBCs الإنزيم اللى بتمل على إنتاجه هو + +498 +00:36:39,980 --> 00:36:43,200 +عبارة عن Alpha Galactosidase لإن أحد مكونات ال + +499 +00:36:43,200 --> 00:36:47,120 +antigen اللى موجود على ال RBCs اللى خاص بالفصيلة B + +500 +00:36:47,120 --> 00:36:52,260 +هو عبارة عن Galactose فانا لو جبتله نوع يعمل لي + +501 +00:36:52,260 --> 00:36:55,740 +Galactase اللى هو إنزعيم يكسر هذا نوع فطرقيا رح + +502 +00:36:55,740 --> 00:36:59,430 +تتحول إلى فصيلة O لإن فصيلة O خاليةتماما من أي + +503 +00:36:59,430 --> 00:37:02,410 +واحد من ال antigenats هذا بتحدث ليه؟ لإنه طبعا ال + +504 +00:37:02,410 --> 00:37:06,190 +goodbye A ب O blood group راح يتحدث بشكل عام بيحكي + +505 +00:37:06,190 --> 00:37:09,570 +لإنه الناس الفاصلة دمهم O عندهم antigenats على ال + +506 +00:37:09,570 --> 00:37:12,810 +RBCs من نوع A الفاصلة دمهم A عندهم antigenats من + +507 +00:37:12,810 --> 00:37:16,550 +نوع A والفاصلة دمهم B عندهم antigenats من نوع B + +508 +00:37:16,550 --> 00:37:20,250 +والفاصلة دمهم AB عندهم antigenats من نوع A و من + +509 +00:37:20,250 --> 00:37:24,500 +نوع B أما ال Oاللي هو الناعي فرقية نوربيه يعني لا + +510 +00:37:24,500 --> 00:37:28,640 +عندهم هذا ولا عندهم هذا النوع التكنيك بشكل مختصر و + +511 +00:37:28,640 --> 00:37:33,060 +بشكل سريع طبعا بتعملي على إنتاج كما حكينا نوع من + +512 +00:37:33,060 --> 00:37:35,840 +أنواع الأنظمات هو عبارة عن القلاد أنفا + +513 +00:37:35,840 --> 00:37:39,120 +-galactosidase بيروح بتعملي cut of the sugars in + +514 +00:37:39,120 --> 00:37:42,850 +surface of RBCs الخاص في دينالنوع التاني من + +515 +00:37:42,850 --> 00:37:46,510 +البكتيريا مابتصلناش فيه لإنه اقتصرا للوقت هو عبارة + +516 +00:37:46,510 --> 00:37:49,610 +عن ال Elizabeth King Young Hingospectrum أو الاسم + +517 +00:37:49,610 --> 00:37:51,950 +التاني المتعرف عليه أكتر هو عبارة عن + +518 +00:37:51,950 --> 00:37:54,610 +الفلافوبكتيريا من Hingospectrum النوع التاني من + +519 +00:37:54,610 --> 00:37:59,280 +البكتيريا بيحول فصلة الدم A إلى فصلة Oكيف؟ طبعا + +520 +00:37:59,280 --> 00:38:02,880 +نفس الطريقة بتعملى على إنتاج إنزيم خاص اسمه N + +521 +00:38:02,880 --> 00:38:06,480 +-acetylgalactamalanase + +522 +00:38:06,480 --> 00:38:10,320 +بتحول فاصلة الدم A إلى الفاصلة O بتخلص من ال + +523 +00:38:10,320 --> 00:38:14,240 +sugars الموجودة على ال surface لل RBCs و فورا + +524 +00:38:14,240 --> 00:38:18,280 +تتحول إلى الفاصلة Oهنا طبعا بشكل مختصر حكالة عن + +525 +00:38:18,280 --> 00:38:22,940 +البكتيريا desfragilosa بتعملى على إزالة أنتجين بيه + +526 +00:38:22,940 --> 00:38:25,000 +و بالنسبة للنوع التانى هو عبارة عن ال Elizabeth + +527 +00:38:25,000 --> 00:38:29,340 +King-Yamingospectrum بتتخلص من الفصيلة A طبعا هذا + +528 +00:38:29,340 --> 00:38:32,740 +النوع من البكتيريا برضه موجود in normal flora in + +529 +00:38:32,740 --> 00:38:35,180 +the intestinal tract و في حالة ال abnormal + +530 +00:38:35,180 --> 00:38:39,540 +conditions بتكون endogenous أو بتسببلى يعني أمراض + +531 +00:38:39,540 --> 00:38:43,210 +و infectionsقولنا كيف بدنا نستفيد او إيش السبب من + +532 +00:38:43,210 --> 00:38:47,330 +إنه اكتشفوا هذه الأنزيمات في تحويل فصائل لدم لإن + +533 +00:38:47,330 --> 00:38:50,750 +في عندي مثلا أنا مريض فصيلة دم بيه و الفصيلة + +534 +00:38:50,750 --> 00:38:53,190 +موجودة عندك في التلاجة مثلا أو المحفوظة عندك + +535 +00:38:53,190 --> 00:38:56,690 +فصيلتها ايه طب أنا كيف بدي أوصل ايه لبيه و هذا + +536 +00:38:56,690 --> 00:38:59,710 +الشخص في حالة مثلا مرض شديد أو لازم يعني أني + +537 +00:38:59,710 --> 00:39:03,570 +أجيبله فصيلة دم و إليه حيموت فكورا بستعمل أنزيمات + +538 +00:39:03,570 --> 00:39:06,770 +هذا النوع من البكتيريا و بحطها على هذا الدم طبعا + +539 +00:39:06,770 --> 00:39:10,310 +مش من شغلنا احنا من شغل تكاترةو فبتتحول تلقائيًا + +540 +00:39:10,310 --> 00:39:13,210 +فاصلة الدم إلى O متعارفة عليه طبعًا هي عبارة عن ال + +541 +00:39:13,210 --> 00:39:17,490 +universe blood cells يعني انها عالمية انها بتقدر + +542 +00:39:17,490 --> 00:39:22,290 +توزعلي الدم لجميع الفصائل التالية لإن O بتاعة B و + +543 +00:39:22,290 --> 00:39:26,790 +بتاعة A بتاعة AB طبعا فهيك تلقائيًا بتتحول فاصلة + +544 +00:39:26,790 --> 00:39:30,550 +الدم إلى O و بقدرزيتها أنا اسعف هذا المريض فبعطيه + +545 +00:39:30,550 --> 00:39:34,850 +من هذا الدم فبالتالي بتكون safety of clinical + +546 +00:39:34,850 --> 00:39:37,690 +transfusion شكرا لكم اسمي اسمي + +547 +00:39:41,820 --> 00:39:45,020 +طبعا اليوم هنحكي عن بكتيريا مهمة و تم اكتشافها + +548 +00:39:45,020 --> 00:39:50,280 +حديثا اسمها ال humans bacteria اول + +549 +00:39:50,280 --> 00:39:52,960 +شغل قبل ما نتعرف عن خصائص هذه البكتيريا اننا نعرف + +550 +00:39:52,960 --> 00:39:55,660 +ان هذه البكتيريا في عندها ميكانيزم خاص بتقوم فيه + +551 +00:39:55,660 --> 00:39:59,100 +اللي هو ال bioluminescence اول اشي هذه البكتيريا + +552 +00:39:59,100 --> 00:40:05,080 +هذه الخاصية بتقوم ببعث ضوء بتعمل emission للضوء + +553 +00:40:05,080 --> 00:40:08,540 +طبعا تعمل emission للضوء بوسط انزيم اسمه ال + +554 +00:40:08,540 --> 00:40:13,900 +luciferaseبحكي إن هذه البكتيريا بتكون موجودة + +555 +00:40:13,900 --> 00:40:17,820 +بأشياء كتيرة زي الفنجاي و طبعا المسكرون بتكون + +556 +00:40:17,820 --> 00:40:21,560 +موجودة بالبكتيريا و بتكون موجودة على ال gut tract + +557 +00:40:21,560 --> 00:40:23,920 +و ال skin surface لبعض ال marine اللي موجودة في + +558 +00:40:23,920 --> 00:40:29,840 +المحيطات و البحار هذه صور بتوضح لنا كيف البكتيريا + +559 +00:40:29,840 --> 00:40:30,700 +بتعطي ضوء + +560 +00:40:34,050 --> 00:40:37,290 +طبعا هذه البكتيريا منشورة بشكل واسع زي ما حكينا في + +561 +00:40:37,290 --> 00:40:40,190 +ال sea water و في ال sheens المحيطات و بال + +562 +00:40:40,190 --> 00:40:44,370 +freshwater كمان بيقول إن هذه البكتيريا قادرة على + +563 +00:40:44,370 --> 00:40:48,390 +إنها تعمل علاقة تكافلية مع بعض الكائنات البحرية زي + +564 +00:40:48,390 --> 00:40:52,390 +ال fish و ال scutes اللي هي السرطانات ال crepes و + +565 +00:40:52,390 --> 00:40:58,470 +الحبار و ال dolphins بعض خصائص البكتيريا إن هي + +566 +00:40:58,470 --> 00:41:02,610 +root sap و كمان gram negative و كمان هي البكتيريا + +567 +00:41:02,610 --> 00:41:09,000 +ديموتايو طبعا نوع المتايل flattened motion و طبعا + +568 +00:41:09,000 --> 00:41:19,760 +هي facultative in aerobics هذا + +569 +00:41:19,760 --> 00:41:22,020 +ال mechanism اللي بتقوم فيه البكتيريا فيه نسط + +570 +00:41:22,020 --> 00:41:26,940 +بسترات اسمها ال luciferin بوجود الأكسوجين بانزيم + +571 +00:41:26,940 --> 00:41:34,180 +اسمه ال luciferase بيعطينا ال lightهذه الميكانيزم + +572 +00:41:34,180 --> 00:41:37,340 +تبعتها طبعا بيحكي لإن كل بكتيريا .. هذه البكتيريا + +573 +00:41:37,340 --> 00:41:41,620 +عندها قدرة على إنتاج ال .. ال auto-inducer كل + +574 +00:41:41,620 --> 00:41:44,560 +بكتيريا بتعطيني auto-inducer طبعا ال auto-inducer + +575 +00:41:44,560 --> 00:41:48,940 +هذا احنا لما أخدنا خاصية ال biofilm في البكتيريا + +576 +00:41:48,940 --> 00:41:52,660 +عندها القدرة على عمل keram sensing فكل بكتيريا + +577 +00:41:52,660 --> 00:41:55,760 +بتعطيني auto-inducer واحد واللي بنظم هذه العملية + +578 +00:41:55,760 --> 00:42:01,980 +في عندنا gene اسمه Lux operin geneطبعا بيحكيلي ال + +579 +00:42:01,980 --> 00:42:05,820 +substrate بتاعة البكتيريا هذه في عندنا اللي هي + +580 +00:42:05,820 --> 00:42:10,780 +flavin mononucleotide مع ال O2 و بوجود ال + +581 +00:42:10,780 --> 00:42:15,520 +longitude من ال fatty aldehyde طبعا بالنهاية + +582 +00:42:15,520 --> 00:42:18,860 +بيعطيني طاقة و بيعطيني ضوء و طبعا الضوء اللي + +583 +00:42:18,860 --> 00:42:22,680 +بتعطيني هذه البكتيريا اللي هو blue green و ممكن + +584 +00:42:22,680 --> 00:42:29,620 +نشوفه على طول موج 490 nanometerهذا الكلام حكيناه + +585 +00:42:29,620 --> 00:42:34,020 +طوال طبعا الأصناف تبعت هذه البكتيريا تلت أصناف تم + +586 +00:42:34,020 --> 00:42:37,260 +اكتشافها اللي هي ال vibro و ال photo photo + +587 +00:42:37,260 --> 00:42:42,340 +bacterium و ال photo hydrous طبعا ال vibro في منها + +588 +00:42:42,340 --> 00:42:45,760 +نوعين ال fisher و ال .. و ال hearty + +589 +00:42:48,270 --> 00:42:51,050 +الميديات اللى بنستخدمها عشان ننمي هذه البكتيريا + +590 +00:42:51,050 --> 00:42:53,310 +الميديا مرت علينا الثيوسالفيتسايتريت + +591 +00:42:53,310 --> 00:42:57,550 +بايلسولتساكاروز ال TCPC-Agar والميديا التانية ال + +592 +00:42:57,550 --> 00:43:00,930 +modified sea water complete وطبعا بتاعتها لون أخضر + +593 +00:43:00,930 --> 00:43:06,270 +و أزرق على الميديا هاي صورة للميديا بنمي عليها + +594 +00:43:06,270 --> 00:43:10,510 +البكتيريا و هاي الألوان زى ما بتشايفين مش واضح + +595 +00:43:10,510 --> 00:43:13,290 +كتير أزرق و أخضر بيكون اللون + +596 +00:43:15,630 --> 00:43:18,530 +بعض التطبيقات لهذه البكتيريا بيقول إنهم استخدموها + +597 +00:43:18,530 --> 00:43:22,150 +في مجال ال food microbiology كمان استخدموها ك + +598 +00:43:22,150 --> 00:43:25,230 +biotechnology و استخدموها في ال drug diagnosis و + +599 +00:43:25,230 --> 00:43:29,050 +كمان هذه البكتيريا بيقول إنها بتعمل تقليل على ال + +600 +00:43:29,050 --> 00:43:34,390 +toxicity للwater و كمان تم اكتشافها في .. طبعا هذه + +601 +00:43:34,390 --> 00:43:38,090 +البكتيريا بتكشف عن موجود ال fossil fuels اللي هو + +602 +00:43:38,090 --> 00:43:41,670 +الوقود الوحفوري و بتستخدم في ال cold war اللي هي + +603 +00:43:41,670 --> 00:43:45,920 +الحرب الباردةطبعا هذه من مساويها و كمان بيقولي في + +604 +00:43:45,920 --> 00:43:48,720 +دراسة حديثة بتحكي أنهم استخدموا هذه البكتيريا في + +605 +00:43:48,720 --> 00:43:52,340 +إنارة الطرق و الشوارع و يعني هذا تطبيق جيد لو إحنا + +606 +00:43:52,340 --> 00:43:56,040 +استخدمناه و في دراسات كتير تمت على هذه البكتيريا و + +607 +00:43:56,040 --> 00:43:59,480 +ان شاء الله بيتم هنا دراسة بغزة و بيلقوا في البحر + +608 +00:43:59,480 --> 00:44:02,620 +تبعنا و بيستخلصوا البكتيريا و بنقدر إنه نضيق + +609 +00:44:02,620 --> 00:44:05,200 +الشوارع بدون .. وش كده؟ + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/D_0va0lT7Sw_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/D_0va0lT7Sw_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..a8beb5d77f3f6257a5e67a3d168d4dea6f13b01a --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/D_0va0lT7Sw_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 4182, "start": 20.62, "end": 41.82, "text": "بسم الله الرحمن الرحيم اتوقفنا المرة الماضية عند listetrogasnemonia شرحنا بعض النقاط المتعلقة فيها لبقص انه يتمور عليها مروسيا ان شاء الله قولنا هى gram-bostef-deblogokai زيها زي 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{"start": 90.16, "end": 90.34, "word": " بال", "probability": 0.923828125}, {"start": 90.34, "end": 90.94, "word": " vaccination", "probability": 0.87060546875}, {"start": 90.94, "end": 92.06, "word": " و", "probability": 0.990234375}, {"start": 92.06, "end": 92.14, "word": " في", "probability": 0.689453125}, {"start": 92.14, "end": 92.28, "word": " ال", "probability": 0.98681640625}, {"start": 92.28, "end": 93.26, "word": " identification", "probability": 0.96875}, {"start": 93.26, "end": 93.68, "word": " برضه", "probability": 0.9775390625}, {"start": 93.68, "end": 94.18, "word": " بنساعده", "probability": 0.87314453125}, {"start": 94.18, "end": 94.6, "word": " من؟", "probability": 0.556884765625}, {"start": 94.6, "end": 95.42, "word": " الكابسول", "probability": 0.916259765625}], "temperature": 1.0}, {"id": 4, "seek": 12072, "start": 96.12, "end": 120.72, "text": "في ال vaccination قلنا اننا مشكلة حقيقية 84 serotype فينا وبالتالي مش منطقي نعطي واحد vaccine بيحتوي على 84 serotype لذلك 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"text": "هذا اللي هو الشائع اللي هو الصورة هذه هي مستعمرات للبكتيريا على الطباط اضافنا قطرة من ال bio على الطباط اللي هو blood agar اختفت المستعمرات تماما ذهبت مع ذلك الحكي انها bio soluble bio soluble اذكركوا بال in terracotta بالستر كوكاس فيريدانس بما عنها انها negative for all other tests", "tokens": [3224, 15730, 13672, 1829, 31439, 25124, 16373, 3615, 13672, 1829, 31439, 31767, 13063, 3660, 29538, 39896, 3714, 14851, 25957, 2288, 9307, 24976, 3555, 4117, 2655, 13546, 25528, 15844, 41950, 3555, 41193, 1975, 11242, 31845, 8315, 12174, 9566, 25720, 9154, 2423, 12198, 15844, 41950, 3555, 41193, 13672, 1829, 31439, 3390, 623, 289, 1975, 46456, 5172, 2655, 9673, 14851, 25957, 2288, 9307, 46811, 10943, 995, 29910, 3224, 3555, 2655, 20449, 29910, 23275, 21542, 4117, 1829, 16472, 11296, 12198, 24807, 638, 12198, 24807, 638, 1975, 8848, 37983, 4117, 14407, 20666, 294, 1796, 12080, 22967, 20666, 14851, 2288, 9122, 2407, 4117, 32277, 8978, 16572, 3215, 7649, 3794, 4724, 15042, 18871, 11296, 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بكتيريا النستاتين نستخدمه لمنع الفطريات", "tokens": [53, 282, 1112, 88, 20021, 383, 12610, 1829, 14851, 9957, 4032, 1863, 14851, 9307, 9957, 23032, 3555, 3615, 995, 2423, 8979, 1112, 88, 20021, 8717, 14851, 9778, 40448, 3224, 5296, 27842, 3615, 25724, 2288, 10943, 3353, 4724, 4117, 2655, 13546, 25528, 33251, 12610, 1829, 14851, 9957, 8717, 14851, 9778, 40448, 3224, 5296, 27842, 3615, 25724, 2288, 10943, 3671, 4724, 4117, 2655, 13546, 25528, 28239, 14851, 9307, 9957, 8717, 14851, 9778, 40448, 3224, 5296, 27842, 3615, 27188, 9566, 16572, 9307], "avg_logprob": -0.289453112706542, "compression_ratio": 1.963768115942029, "no_speech_prob": 0.0, "words": [{"start": 1556.7899999999997, "end": 1557.9499999999998, "word": "Vancomycin", "probability": 0.6517578125}, {"start": 1557.9499999999998, "end": 1559.11, "word": " Cوليستين", "probability": 0.55384521484375}, {"start": 1559.11, "end": 1560.85, "word": " ونستاتين", "probability": 0.75439453125}, {"start": 1560.85, "end": 1561.81, "word": " 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+00:00:46,140 --> 00:00:48,700 +الشكل الكوكاى اللى عن طبق عليها حقيقة قلنا انها + +8 +00:00:48,700 --> 00:00:53,140 +تشبه الكوكوبسلاي أكتر لكن يعني شبهنها بكلة القدم + +9 +00:00:53,140 --> 00:00:55,880 +الأمريكية اللى هي البيضاوية فنتين جانب بعض + +10 +00:00:55,880 --> 00:01:00,900 +بالاضافة إلى وجود capsule قضينا + +11 +00:01:00,900 --> 00:01:04,560 +يعني جل المحاضرة السابقة اتحدثت عن الكبسول وعن + +12 +00:01:04,560 --> 00:01:07,600 +اليومالايسين وبعض ال service protein والتيكويك أسد + +13 +00:01:08,060 --> 00:01:11,160 +و ال .. بيبتدوا why كان fragments و كيف تأثيرهم + +14 +00:01:11,160 --> 00:01:15,580 +على جهاز المناعة و اتحدثنا عن حاجة اسمها auto + +15 +00:01:15,580 --> 00:01:19,740 +-license auto-license بعدين أفرضنا في ال .. ال .. + +16 +00:01:19,740 --> 00:01:24,600 +الصفحة كاملة لحديث عن الكابسول واستفدنا من + +17 +00:01:24,600 --> 00:01:27,480 +الكابسول المجموعة من المعلومات الصحية قولنا انه ال + +18 +00:01:27,480 --> 00:01:30,940 +vaccination احنا بنساعد بالكابسول بال vaccination + +19 +00:01:30,940 --> 00:01:36,300 +و في ال identification برضه بنساعده من؟ الكابسولفي + +20 +00:01:36,300 --> 00:01:39,860 +ال vaccination قلنا اننا مشكلة حقيقية 84 serotype + +21 +00:01:39,860 --> 00:01:44,540 +فينا وبالتالي مش منطقي نعطي واحد vaccine بيحتوي + +22 +00:01:44,540 --> 00:01:50,900 +على 84 serotype لذلك بندور في البلد ال most common + +23 +00:01:50,900 --> 00:01:54,020 +.. بالظبط ال most common serotypes و بنجمعهم و + +24 +00:01:54,020 --> 00:01:56,880 +بنعطي vaccine لهم فانا في واحد مشهور ب 14 + +25 +00:01:56,880 --> 00:02:01,830 +serotypes يعني يحتوي على 14 serotypesفي ال + +26 +00:02:01,830 --> 00:02:05,310 +identification عشان انا اعرف مين ال common مش لازم + +27 +00:02:05,310 --> 00:02:09,290 +برضه نعمل لما بعزل استيطوكوس نيمونية اتعرف على + +28 +00:02:09,290 --> 00:02:12,710 +zero types وبالتالي لازم استخدم برضه specific + +29 +00:02:12,710 --> 00:02:16,830 +antiserum لتعرف على اللي هو ال capsular zero types + +30 +00:02:16,830 --> 00:02:22,470 +اخر جملة كانت اللي دججنا عليها ودلوقت اللي هي only + +31 +00:02:22,470 --> 00:02:26,390 +for susceptible population ما معنى susceptible + +32 +00:02:26,390 --> 00:02:31,230 +population خليني اوضح بس المفهوم عشان منا مانسوش + +33 +00:02:32,300 --> 00:02:37,660 +تسمع بمرض يصيب الأطفال، مرض يصيب الكبار السن، مرض + +34 +00:02:37,660 --> 00:02:42,120 +يصيب فقط مرض ال aids، مرض لا يصيب اللي عندهم + +35 +00:02:42,120 --> 00:02:48,710 +anemiaالملاريا مثلا مابتصيبش الناس ان عندهم أنيميا + +36 +00:02:48,710 --> 00:02:52,550 +الخلايا المنجلية sickles الانيميا تمام؟ يبقى في + +37 +00:02:52,550 --> 00:02:56,090 +susceptible groups و في non susceptible groups + +38 +00:02:56,090 --> 00:03:00,750 +اللي احنا المرة قولناها يعني زي ال host + +39 +00:03:00,750 --> 00:03:04,430 +specificity على سبب المثال لكن هنا بنتكلم عن + +40 +00:03:04,430 --> 00:03:09,090 +الإنسان هو كله susceptible لكن في فئات أكتر أرض + +41 +00:03:09,090 --> 00:03:12,750 +للإصابة مثلا احنا في مجال التحديات الطبيةفي + +42 +00:03:12,750 --> 00:03:17,190 +المستشفيات are susceptable group to be infected + +43 +00:03:17,190 --> 00:03:22,770 +with blood-borne viruses زي مثلا ال hepatitis B و + +44 +00:03:22,770 --> 00:03:25,710 +ال hepatitis C على سبيل المثال تمام؟ blood-borne + +45 +00:03:25,710 --> 00:03:29,410 +diseases مش أكتر وضع من الغاز والإيبارا بتاع ليه؟ + +46 +00:03:29,410 --> 00:03:33,150 +احنا أكتر وضع للإصابة تكلمة susceptable population + +47 +00:03:33,150 --> 00:03:37,630 +هم للفئة الأكتر عرضة الأكتر عرضة قد يكون الأطفال + +48 +00:03:37,630 --> 00:03:39,430 +قد يكون مرضى بورض معين الاخر + +49 +00:03:42,990 --> 00:03:45,770 +احنا تحدثنا عن ال Structococcus pneumonia و عن ال + +50 +00:03:45,770 --> 00:03:48,350 +violence factors قبل أن نتحدث عن الأمور تباعتها هي + +51 +00:03:48,350 --> 00:03:52,210 +المسمى تبعها ال disease pneumonia و looper + +52 +00:03:52,210 --> 00:03:54,810 +pneumonia فاكرين looper pneumonia لكن بتكونوا + +53 +00:03:54,810 --> 00:03:58,790 +تعرفوا إنها غالبا مش هي ال primary cause of + +54 +00:03:58,790 --> 00:04:04,010 +pneumonia إيش هي ال primary cause؟ يعني في بعض + +55 +00:04:04,010 --> 00:04:07,410 +الحاجة إنه فيها ممكن يكون secondary cause أعطيكم + +56 +00:04:07,410 --> 00:04:11,810 +مثال بسيطالان عندنا واحد مرض بال influenza و ال + +57 +00:04:11,810 --> 00:04:15,330 +influenza هي upper respiratory tract infection و + +58 +00:04:15,330 --> 00:04:17,770 +قد تصل إلى اللي هو ال respiratory tract في ال + +59 +00:04:17,770 --> 00:04:19,890 +upper respiratory tract بيصير فيه destruction لل + +60 +00:04:19,890 --> 00:04:22,870 +epithelial cells إذا صارت destruction لل + +61 +00:04:22,870 --> 00:04:25,710 +epithelial cells ل scriptococcus pneumonia اللي + +62 +00:04:25,710 --> 00:04:30,490 +كانت موجودة normal flora في أفواهكم ممكن تعمل باطل + +63 +00:04:30,490 --> 00:04:31,290 +بعدين + +64 +00:04:42,240 --> 00:04:46,240 +مين ممكن يصاب أو يسلم ال destruction لل + +65 +00:04:46,240 --> 00:04:51,960 +respiratory epithelial cells ممكن اللي عندهم viral + +66 +00:04:51,960 --> 00:04:55,540 +infection اللي عندهم viral infection المدخنين + +67 +00:04:55,540 --> 00:04:58,180 +يسمونهم ال chain smokers اللي هم ال heavy smokers + +68 +00:04:58,180 --> 00:05:03,920 +اللي بدخنوا بشكل كبيرمن يعملون في مصانع فيها + +69 +00:05:03,920 --> 00:05:07,700 +irritants إيش يعني irritants؟ موارد مهيجة بتسمعوا + +70 +00:05:07,700 --> 00:05:11,160 +ال .. ال .. ال boya ال formal .. ال formal .. ال + +71 +00:05:11,160 --> 00:05:16,080 +formaldehyde كتير من المركبات قد تسبب destruction + +72 +00:05:16,080 --> 00:05:19,040 +للخلايا وإن لابتكن ال virus دولة لابتكن إيه؟ + +73 +00:05:19,040 --> 00:05:22,960 +بكتيريا كل هؤلاء الناس بيكونوا أكثر أرض الإصابة من + +74 +00:05:22,960 --> 00:05:25,360 +اللي هم الناس العاديين اللي ما عندهم أي إشكالية في + +75 +00:05:25,360 --> 00:05:30,120 +ال upper respiratory tract ممكن تسبب إنهاMiddle + +76 +00:05:30,120 --> 00:05:34,000 +Ear Infection ومشهور جدا كـ Middle Ear Infection + +77 +00:05:34,000 --> 00:05:38,480 +وبنسميها احنا دي Otitis Media Otitis Media التهاب + +78 +00:05:38,480 --> 00:05:43,580 +الأذن الوسطى التهاب الأذن الوسطى في أن ال .. ال .. + +79 +00:05:43,580 --> 00:05:49,240 +ال بكتيريميا تنتج بعد إصابة أولية مثلا pneumonia + +80 +00:05:49,240 --> 00:05:53,800 +أو Otitis Media إذا صار في بكتيريميا و الرسم مجري + +81 +00:05:53,800 --> 00:05:59,040 +بتخلص منها أو يتخلص من الميكروباتتتطور إلى + +82 +00:05:59,040 --> 00:06:03,760 +meningitis انا قلتلكوا المحاضرة الماضية ان ال + +83 +00:06:03,760 --> 00:06:10,660 +vaccines اللي استخدمت عملت انها حاجة إيجابية انها + +84 +00:06:10,660 --> 00:06:13,380 +قلرت الإصابة بمرض ال meningitis due to + +85 +00:06:13,380 --> 00:06:16,960 +streptococcus pneumonia نعيد من اللوحة نفسها ال + +86 +00:06:16,960 --> 00:06:21,960 +clinical findingsقلنا إنه أشهر disease بسببه هو + +87 +00:06:21,960 --> 00:06:25,800 +pneumonia وقلنا غالبا هو عبارة عن secondary to + +88 +00:06:25,800 --> 00:06:28,760 +viral infections أو destruction by chemicals أو + +89 +00:06:28,760 --> 00:06:32,200 +irritants أخرىلكن هناك أمراض أخرى بتسببها + +90 +00:06:32,200 --> 00:06:35,200 +استرتكوكاس نيمونيا اللي من ضمنها ال autitis media + +91 +00:06:35,200 --> 00:06:39,360 +و ال .. ال bacterium and meningitis و قلنا إن ال + +92 +00:06:39,360 --> 00:06:43,640 +vaccine .. ال vaccine اللي وظف في ال 2011 في قطاع + +93 +00:06:43,640 --> 00:06:48,600 +غزة سيعمل على خفض حالات من ال meningitis اللي هي + +94 +00:06:48,600 --> 00:06:51,500 +أصلا غالبا نجم عن من؟ عن ال pneumonia أو عن + +95 +00:06:51,500 --> 00:06:57,230 +autitis media في الأطفالمن أشهر الفحوصات اللى + +96 +00:06:57,230 --> 00:07:00,970 +بنستخدمها لتعريف البكتيريا فحص mobile solubility + +97 +00:07:00,970 --> 00:07:05,590 +فاكرين ال auto lysin لما البكتيريا بتشعر بتهديد + +98 +00:07:05,590 --> 00:07:08,970 +بتموت لحالها تقولنا كفى الله اللي ممكن نقتل ماحدش + +99 +00:07:08,970 --> 00:07:13,710 +يعني يعني يحاول أنا لحالي بدأت بالأمور ال ال ال + +100 +00:07:13,710 --> 00:07:18,810 +bio معروف عنه معروف عنهإنه عنده قدرة على قتل + +101 +00:07:18,810 --> 00:07:22,590 +البكتيريا و الدليل على درجة أننا أضفناه على + +102 +00:07:22,590 --> 00:07:27,750 +المقنكي to inhibit the growth of gram poster طبعا + +103 +00:07:27,750 --> 00:07:29,650 +مش كل ال gram poster شوفت في المحاضرة الماضية ال + +104 +00:07:29,650 --> 00:07:32,550 +enterococcus تنمو في ال bio تمام؟ بتقاوم ال bio + +105 +00:07:32,550 --> 00:07:36,090 +لذلك كل من ينمو في الأمعاق غالبا لازم يكون عنده + +106 +00:07:36,090 --> 00:07:40,370 +نوع من المقاومة له ال bio البكتيريا التنهيه مالهاش + +107 +00:07:40,370 --> 00:07:44,300 +أي مقاومةالصورتين اللي قد .. او .. او الأنبوبتين + +108 +00:07:44,300 --> 00:07:47,060 +اللي قدامكوا على اللوحة يا تركز على اللوحة واحدة + +109 +00:07:47,060 --> 00:07:48,560 +مكتوبة عليها positive واحدة مكتوبة عليها negative + +110 +00:07:48,560 --> 00:07:52,180 +ال positive خالية تماما من النمو خالية تماما من ال + +111 +00:07:52,180 --> 00:07:55,820 +turbidity بينما ال negative مليئة ب turbidity + +112 +00:07:55,820 --> 00:07:59,980 +ودليل ع ذلك ان احنا شايفين ال .. ال أسلاك بتاعة ال + +113 +00:07:59,980 --> 00:08:03,860 +back من خلالها في ال positive ومش شايفين الأسلاك + +114 +00:08:03,860 --> 00:08:07,920 +في اللي هي ال .. ماعنى عنى ذلك هي فرع نبوبة ووضعنا + +115 +00:08:07,920 --> 00:08:13,380 +فيها بكتيرياواحدة من الاتنين اتنين او او او عفوا + +116 +00:08:13,380 --> 00:08:17,060 +حاطين على واحدة منهم بال و التانية اللي حاطين + +117 +00:08:17,060 --> 00:08:20,960 +عليها بال بعد ربع ساعة طلعوا يشتروا في الموبا اللي + +118 +00:08:20,960 --> 00:08:24,580 +حاطين عليها بال راحت ال turbidity دلوقتي علاش ان + +119 +00:08:24,580 --> 00:08:30,360 +البكتيريا ناتت و اتحللت و ذابت و بيسمو الفحص bi + +120 +00:08:30,360 --> 00:08:32,180 +solubility test + +121 +00:08:37,720 --> 00:08:40,860 +هذا اللي هو الشائع اللي هو الصورة هذه هي مستعمرات + +122 +00:08:40,860 --> 00:08:45,140 +للبكتيريا على الطباط اضافنا قطرة من ال bio على + +123 +00:08:45,140 --> 00:08:49,000 +الطباط اللي هو blood agar اختفت المستعمرات تماما + +124 +00:08:49,000 --> 00:08:56,500 +ذهبت مع ذلك الحكي انها bio soluble bio soluble + +125 +00:08:56,500 --> 00:09:00,820 +اذكركوا بال in terracotta بالستر كوكاس فيريدانس + +126 +00:09:00,820 --> 00:09:06,770 +بما عنها انها negative for all other testsهي + +127 +00:09:06,770 --> 00:09:10,990 +negative for bi , negative for bi-solubility هذا + +128 +00:09:10,990 --> 00:09:13,090 +الفحص ميز من التنسيق، يعني ال spirit of coca + +129 +00:09:13,090 --> 00:09:16,510 +cinnamonia بيعيش bi-soluble بينما ال viridance + +130 +00:09:16,510 --> 00:09:21,670 +group non-bi أو non-soluble طب في كمان فحص كتير + +131 +00:09:21,670 --> 00:09:24,250 +مشهور، إذا ذكرت ال spirit of coca cinnamonia أيضا + +132 +00:09:24,250 --> 00:09:28,170 +يذكر، optochain disk وoptochain disk هو عبارة عن + +133 +00:09:28,170 --> 00:09:33,930 +إيه؟ عبارة عن antibiotic diskزي اللي استخدمناه في + +134 +00:09:33,930 --> 00:09:38,690 +الـ differentiation بين Staphylococcus epidermidis + +135 +00:09:38,690 --> 00:09:41,350 +و Staphylococcus saprophytic اتعرف ايه اسمه كده + +136 +00:09:41,350 --> 00:09:47,230 +الفحص؟ ان هو بيوزي، تمام؟ هذا ابتشين بيميز بين + +137 +00:09:47,230 --> 00:09:50,770 +Staphylococcus pneumonia و Staphylococcus viridans + +138 +00:09:50,770 --> 00:09:54,470 +ال pneumonia sensitive و لاحظوا ال zone of + +139 +00:09:54,470 --> 00:09:58,550 +inhibition اللي حوالين القرص واضح ان في NDA + +140 +00:09:58,550 --> 00:10:03,950 +killing لالبكتيرياأول بكتيريا اللي هي استرددو كوكس + +141 +00:10:03,950 --> 00:10:08,470 +نيمونيا ال sensitive ال viridance group فوق مافيش + +142 +00:10:08,470 --> 00:10:11,990 +اي zone of inhibition لان هي resistance فهي فحسين + +143 +00:10:11,990 --> 00:10:16,930 +بميزة ال viridance عن مين؟ عن النيمونيا، bisoluble + +144 +00:10:16,930 --> 00:10:22,170 +وoptocine resistance طبعا، بينما ال استرددو كوكس + +145 +00:10:22,170 --> 00:10:28,460 +نيمونيا bisoluble وoptocine sensitiveتيجي بقى فحص + +146 +00:10:28,460 --> 00:10:32,340 +مشهور اسمه Quilang reaction او له اسم تاني + +147 +00:10:32,340 --> 00:10:37,340 +capsular swelling test swelling ايش معنى swelling؟ + +148 +00:10:37,340 --> 00:10:42,860 +swell انتفاق راكزوا بقى هاي الصورة اللي أمامنا مش + +149 +00:10:42,860 --> 00:10:47,180 +معبرة عن ما أريد قوله شريحة بتاع الشرايح + +150 +00:10:47,180 --> 00:10:52,220 +الميكروسكوبية العادية قطرة من ال saliva البكتيريا + +151 +00:10:52,220 --> 00:10:54,060 +المشتبه فيها mixing + +152 +00:10:58,190 --> 00:11:02,670 +تحت الميكروسكوب بنخفف الإضاءة قدر الإمكان ويعني لو + +153 +00:11:02,670 --> 00:11:06,010 +كان في ال dark field ميكروسكوبي لبأسة في ذلك هنشوف + +154 +00:11:06,010 --> 00:11:10,850 +الدبل الكوكاي و هنشوف الكابسول طبعا جنب ال cover + +155 +00:11:10,850 --> 00:11:14,250 +slip عارفين cover slip اللي فوق الشريحة بنضيف قطرة + +156 +00:11:14,250 --> 00:11:19,310 +من ال capsular antiserum إيش أنا antiserum يعني + +157 +00:11:19,310 --> 00:11:22,610 +antibodies ضد الكابسول تبعت مين تاعت ال striped + +158 +00:11:22,610 --> 00:11:27,100 +coccus pneumoniaطبعا لازم يكون هذا المحلول بيحتوي + +159 +00:11:27,100 --> 00:11:33,120 +على 84 نوع من إيه؟ من ال antibodies لأن القطر اللي + +160 +00:11:33,120 --> 00:11:37,120 +هيش بيصير فيها بتمشي تحت ال cover slip بفعل إن + +161 +00:11:37,120 --> 00:11:40,280 +المسافة اللي بين ال cover slip و الشريحة مسافة + +162 +00:11:40,280 --> 00:11:43,960 +ضئيلة فخاصية شعرية بيصير فيه انتشار لمين؟ لل + +163 +00:11:43,960 --> 00:11:48,720 +antiserum إذا ال antibody ارتبط بال capsule أنا لن + +164 +00:11:48,720 --> 00:11:53,900 +أستطيع رؤية ال antibody أساسافكيف لي ان اعلم انه + +165 +00:11:53,900 --> 00:11:56,840 +صار فيه ارتباط و لا ماصارش فيه ارتباط عن طريق + +166 +00:11:56,840 --> 00:12:00,580 +انتفاخ الكابسولة و عشان هي سموه capsular swelling + +167 +00:12:00,580 --> 00:12:05,360 +test لو صير حجم الكابسولة بناف أكبر بضعفين او ثلاث + +168 +00:12:05,360 --> 00:12:09,500 +أضعاف ال original اللي هو ايه البكتيريا اللي كانت + +169 +00:12:09,500 --> 00:12:12,180 +موجودة هذا المؤشر على ان البكتيريا هي Stetococcus + +170 +00:12:12,180 --> 00:12:16,320 +pneumonia كان مراقب اسمه Guilin reaction اذا نفحص + +171 +00:12:16,320 --> 00:12:19,360 +الان هارفته مهمين لل Stetococcus pneumonia + +172 +00:12:30,190 --> 00:12:32,490 +هذا الفحص اللي قدامنا ليس له علاقة بالـ + +173 +00:12:32,490 --> 00:12:37,870 +Streptococcus pneumonia لكن له علاقة بكل الـ + +174 +00:12:37,870 --> 00:12:41,770 +Streptococcus وذكركم بـ Rebecca Lansfield Rebecca + +175 +00:12:41,770 --> 00:12:46,150 +Lansfield قسمت البكتيريا لـ Streptococcus based on + +176 +00:12:46,150 --> 00:12:52,380 +surface antigen إلى groups A,B,C إلى آخرمحصورة في + +177 +00:12:52,380 --> 00:13:00,220 +a و b و d الان اللى بيحدث بضبط عندنا ان احنا عندنا + +178 +00:13:00,220 --> 00:13:05,720 +ست قنابيف ستة ضالة سبعة واحد اتنين تلاتة اربع خمسة + +179 +00:13:05,720 --> 00:13:09,020 +ستة والسبعة اللي اللي الأحمر دي control control ان + +180 +00:13:09,020 --> 00:13:12,940 +فيها بكتيريا بيها بكتيرياالان هدول عايش المكتوب + +181 +00:13:12,940 --> 00:13:17,880 +Anti-A وAnti-B وAnti-C وAnti-D وAnti-F وAnti-G هم + +182 +00:13:17,880 --> 00:13:20,240 +المرواه اللي احنا بقي نفسنا يبهمونا عزلنا + +183 +00:13:20,240 --> 00:13:23,200 +Striptococcus بنعرف اللي اي نوع بتنتمي هدا ال + +184 +00:13:23,200 --> 00:13:26,940 +Striptococcus فبناخدها و بنحطها على شريحة و + +185 +00:13:26,940 --> 00:13:30,540 +بندوبها في Saline و بنضيف عليها قطرة من Anti-A و + +186 +00:13:30,540 --> 00:13:34,880 +قطرة من Anti-B على .. على .. أماكن مختلفة سبب تامة + +187 +00:13:34,880 --> 00:13:38,940 +هو Grouping Anti-A و Anti-B و Rh تمام؟ و بنعمل + +188 +00:13:38,940 --> 00:13:44,050 +Good Mixing إذا كانالبكتيريا من نوع C هتتفعل مع + +189 +00:13:44,050 --> 00:13:48,370 +مين؟ مع أنتي C وهيعمل انها ايه؟ agglutination طبعا + +190 +00:13:48,370 --> 00:13:54,050 +في كتات كل antigen .. اه أفضل كل antibody بلون + +191 +00:13:54,050 --> 00:13:57,650 +فبالتالي بيسهر علينا عملية اللي هو ال .. إذا مش كل + +192 +00:13:57,650 --> 00:14:00,370 +antibody بلون هم مرتبين ترتيب alphabetically وعنا + +193 +00:14:00,370 --> 00:14:03,970 +شريحة هالمريكوا صورة عنها هي شريحة تانية برضه + +194 +00:14:03,970 --> 00:14:06,590 +مرتبة alphabetically فاحنا ممكن نحط ال + +195 +00:14:06,590 --> 00:14:12,070 +correspondingAntibody على الحرف Anti-A على دائرة A + +196 +00:14:12,070 --> 00:14:14,590 +على دائرة B و هكذا و اللي بيعطينا positive + +197 +00:14:14,590 --> 00:14:18,270 +agglutination test Clumping يعني زي اللي قلت اللي + +198 +00:14:18,270 --> 00:14:21,890 +كنا ال blood group طبعا لكن مش هتشوفوا visible + +199 +00:14:21,890 --> 00:14:26,390 +clumps بشكل واقع زي ال blood group لأن حجم + +200 +00:14:26,390 --> 00:14:30,770 +البكتيريا بقت أزرع من حجم ال blood cellsهذا ال + +201 +00:14:30,770 --> 00:14:35,330 +Latex Agglutination هو فحص معتمد في المختبرات + +202 +00:14:35,330 --> 00:14:39,690 +للتعرف على أنتيجينات اللي هي ال Streptococcus + +203 +00:14:39,690 --> 00:14:42,990 +Prevention and treatment of streptococcus + +204 +00:14:42,990 --> 00:14:49,290 +pneumonia immunity 14 capsule types mixed vaccine + +205 +00:14:49,290 --> 00:14:53,850 +طبعا هنا كلمة prevention مهمة جدا وممكن انا + +206 +00:14:53,850 --> 00:15:00,130 +هأكتلكوا جاب اليك ان ال vaccines أحدثت طورةفي منع + +207 +00:15:00,130 --> 00:15:03,530 +الأمراض المعدية اللى من ضمنها كان ال chickenpox و + +208 +00:15:03,530 --> 00:15:07,570 +ال smallpox و ال polio و ال rubella ال viral + +209 +00:15:07,570 --> 00:15:10,850 +diseases كتيرة ال mycobacterium tuberculosis كمان + +210 +00:15:10,850 --> 00:15:16,030 +اللى هي بال BCG فلما أنا بعطى فاكسين ضد ال + +211 +00:15:16,030 --> 00:15:20,190 +Stretococcus pneumonia تذكروا أنها كانت أحد أسباب + +212 +00:15:20,190 --> 00:15:24,210 +أحد نجوم ال meningitis صح هذا لأ فبالتالي أنا بقلل + +213 +00:15:24,210 --> 00:15:29,470 +نسبة الإصابة بال meningitismost strains suitable + +214 +00:15:29,470 --> 00:15:32,550 +to penicillin but resistance is common هذه النقطة + +215 +00:15:32,550 --> 00:15:39,470 +أجنجها في عندها شوية عشان نوضح زمان كان العلماء + +216 +00:15:39,470 --> 00:15:43,930 +يكتبوا مقالات و يقولوا يحبوا يفسروا لماذا ال + +217 +00:15:43,930 --> 00:15:47,610 +streptococcus ماعندهاش القدرة اللي موجودة عند + +218 +00:15:47,610 --> 00:15:52,090 +الاستفيل الكوكس في ال resistance يعني بمعنى آخر + +219 +00:15:58,180 --> 00:16:00,560 +والدليل على أن الـ tonsillitis was caused by + +220 +00:16:00,560 --> 00:16:04,180 +acetococcus بيونينسك لحد اليوم نسبة كبيرة ممكن + +221 +00:16:04,180 --> 00:16:07,080 +تنجح بعلاجها للبنزينيين العادى، بس هتقول + +222 +00:16:07,080 --> 00:16:09,580 +البنزينيين يعالج الـ staphylococcus which كان + +223 +00:16:09,580 --> 00:16:14,300 +تقريبا 99%، 95% resistant، بينما ال acetococcus + +224 +00:16:14,300 --> 00:16:19,000 +لأ، فضلنا نقول ليش ال acetococcus، طبعا صار في + +225 +00:16:19,000 --> 00:16:19,220 +نضايق + +226 +00:16:23,570 --> 00:16:26,070 +يعني مش شايفيني قدامكوا غير ال Staphylococcus + +227 +00:16:26,070 --> 00:16:29,070 +aureus او ال Staphylococci و انا كمان عندي مفضلة + +228 +00:16:29,070 --> 00:16:34,210 +تبدأ تظهر سلالات مقاومة لمين لل antibiotics لكن + +229 +00:16:34,210 --> 00:16:40,530 +بشكل عام حقيقة هي اقل resistance من مين من لواء ال + +230 +00:16:40,530 --> 00:16:46,370 +Staphylococcus الميكروب الأخير في السلسلة اللى هو + +231 +00:16:46,370 --> 00:16:50,450 +الناسية نقوله نقدر نكمله اليوم عشان نبدأ موضوع + +232 +00:16:50,450 --> 00:16:51,730 +جديد في المرة القادمة + +233 +00:16:54,730 --> 00:16:58,570 +قبل ما ندخل على الموضوع الصورة اللى قدامنا مرعبة ل + +234 +00:16:58,570 --> 00:17:03,030 +gram negative diplococci gram negative diplococci + +235 +00:17:03,030 --> 00:17:06,710 +الموضوع اللى سبقها مباشرة كان gram positive + +236 +00:17:06,710 --> 00:17:09,970 +diplococci ده اللى بيخلطه من grams 10 وبيعتبره من + +237 +00:17:09,970 --> 00:17:14,730 +grams 10 مش يعني حاجة مش تقنية الفرق بين gram + +238 +00:17:14,730 --> 00:17:17,250 +positive و gram negative انها disturbed coccus + +239 +00:17:17,250 --> 00:17:20,210 +نيومونيا وها دي نيسيريا جونريا او نيسيريا من هنا + +240 +00:17:20,210 --> 00:17:25,030 +ده فرق كبيرفرق كبير في ال diagnosis لما انا هتحشو + +241 +00:17:25,030 --> 00:17:28,450 +الكامر شوية نيسيريا مينيديدس من نجوم ال ميرنجايتس + +242 +00:17:28,450 --> 00:17:32,650 +وسترتوكوكاس نيمونيا من نجوم .. لما انا اقول gram + +243 +00:17:32,650 --> 00:17:35,510 +negative دبلو كوكايلة لما اقول gram positive دبلو + +244 +00:17:35,510 --> 00:17:41,830 +كوكايلة ال نيسيريا في ان اسمان مشهورين نيسيريا + +245 +00:17:41,830 --> 00:17:46,310 +جونورية و نيسيريا مينيديدس هم الاسمين الأشهد اطلاق + +246 +00:17:46,310 --> 00:17:49,470 +في هذا ال genus هذا لايعني انه مافيش other نيسيريا + +247 +00:17:51,240 --> 00:17:54,660 +الفيميلز بشكل عام بيحتوى في ال vagina على normal + +248 +00:17:54,660 --> 00:17:57,040 +flora وهذه نقطة بإننا نتذكرها كويس عشان لما نتكلم + +249 +00:17:57,040 --> 00:18:01,180 +عن ال diagnosis فيميلز carry neisiria-meningitis + +250 +00:18:01,180 --> 00:18:05,920 +ولا جونورية، نيسيريا سيكا، نيسيريا فلافا، نيسيريا + +251 +00:18:05,920 --> 00:18:10,240 +لاكتميكا، أسماء ليس لها علاقة بالpathogenesis لكن + +252 +00:18:10,240 --> 00:18:14,740 +موجودة ك normal flora في الفيميل فقط وبالتالي إلها + +253 +00:18:14,740 --> 00:18:16,520 +consequences في ال diagnosis اللي هتكلم عنه كمان + +254 +00:18:16,520 --> 00:18:16,880 +شوية + +255 +00:18:20,010 --> 00:18:23,470 +Diplokokai, pairs of cocci, oxidase, bostrim ال + +256 +00:18:23,470 --> 00:18:27,730 +pairs هدولة بتعرفوا حبات القهوة؟ لما نواجه حبة + +257 +00:18:27,730 --> 00:18:33,070 +قهوة مش منحني هي زي حرف ال D صح؟ حب ال D .. حرف ال + +258 +00:18:33,070 --> 00:18:36,490 +D و حرف ال D، حب القهوة و حب القهوة، ع جبال بعض أو + +259 +00:18:36,490 --> 00:18:40,710 +حبة فصولي يعني، حبة فصولي، بسموها kidney .. kidney + +260 +00:18:41,900 --> 00:18:46,340 +شيب أو kidney bean shape يعني او اللي قولنا ال D + +261 +00:18:46,340 --> 00:18:49,980 +اللي flattened at the adjacent sites يعني المناطق + +262 +00:18:49,980 --> 00:18:54,960 +المتسقة من الحرفين ال D بكونوا ايه؟ flat ايش يعني + +263 +00:18:54,960 --> 00:19:00,620 +flat؟ يعني مسطحين مش دائرين طيب البكتيريا بتنمو + +264 +00:19:00,620 --> 00:19:03,100 +على ال blood agar اه بتنمو على ال blood agar لكن + +265 +00:19:03,100 --> 00:19:07,230 +احنا بنميها غالبا على ال chocolate agarو نجف عند + +266 +00:19:07,230 --> 00:19:09,930 +كلمة chocolate agar بتتكلم عنه شوية عشان احنا + +267 +00:19:09,930 --> 00:19:14,150 +نوضّف بعض الأمور المهمة جدا في هذا المجال ال + +268 +00:19:14,150 --> 00:19:17,070 +chocolate agar ليس له علاقة بالchocolate هو بال + +269 +00:19:17,070 --> 00:19:21,350 +blood تم تعريضه لحرارة فتغير لونه وسط ال chocolate + +270 +00:19:21,350 --> 00:19:26,110 +تمام الان هو blood agar تم تسخينه على 80 درجة + +271 +00:19:26,110 --> 00:19:30,570 +مئوية فتحول اللون من اللون الأحمر المعروف إلى + +272 +00:19:30,570 --> 00:19:33,450 +اللون البني اللي بيشبه ال chocolate اللي بتسميها + +273 +00:19:33,450 --> 00:19:38,970 +جاء من اللون فقط لهطب السؤال اللى بيطرح نفسه blood + +274 +00:19:38,970 --> 00:19:43,430 +agar و chocolate agar هما نفس التركيبة الفرق فى + +275 +00:19:43,430 --> 00:19:47,070 +التحضير واحد حضرنا على 80 درجة مئوية ال blood و + +276 +00:19:47,070 --> 00:19:50,810 +التانى على 50 الى 55 درجة مئوية what is the + +277 +00:19:50,810 --> 00:19:53,390 +difference between the two؟ ليش هلأ بيستخدموا + +278 +00:19:53,390 --> 00:19:57,030 +اغراضها .. بيستخدموا اغراضها؟ بفضل المفروض ان + +279 +00:19:57,030 --> 00:20:01,170 +خلايا الدم بيكون فيها factor لدخلها لما .. لما + +280 +00:20:01,170 --> 00:20:04,350 +اتعرضت لليه طلعت لبرا اللى ماكانش قبل يستفيد منها + +281 +00:20:04,350 --> 00:20:05,730 +لما ان اتصلت + +282 +00:20:12,880 --> 00:20:15,680 +أنا بتوقع ان ال blood اللي خدناه .. خدناه من إنسان + +283 +00:20:15,680 --> 00:20:17,720 +أو خدناه من حيوان تبتعد تسمع عن sheep blood او + +284 +00:20:17,720 --> 00:20:19,980 +horse blood او حتى احنا في غزة غالبا بيستخدم ال + +285 +00:20:19,980 --> 00:20:24,360 +human blood ال human blood هو المدافر في ال .. + +286 +00:20:24,360 --> 00:20:29,360 +للمحتوى فقط اللي بيستخدموه سواء كان sheep او horse + +287 +00:20:29,360 --> 00:20:33,160 +او قولنا مين human blood نتوقع ان يكون فيه مواد + +288 +00:20:33,160 --> 00:20:38,140 +سامة للبكتيرياتوكسيك الدم هو فيه مواد و فيه + +289 +00:20:38,140 --> 00:20:41,820 +antibiotics و فيه complement صح ولا التسخين غالبا + +290 +00:20:41,820 --> 00:20:47,020 +بيعمل destruction لهذه المواد يعني بمعنى اخر بيقلل + +291 +00:20:47,020 --> 00:20:52,160 +من تأثير الدم السام او الضاق او الملل النمو تمام؟ + +292 +00:20:52,160 --> 00:20:56,680 +بيجي تسخين كويس كمان لكن في المقابل التسخين ايضا + +293 +00:20:56,680 --> 00:20:58,040 +كما بتعرفوا انه + +294 +00:21:01,100 --> 00:21:04,260 +بيعمل destruction ليش بس للمواد الضارة، قد يقوم + +295 +00:21:04,260 --> 00:21:12,160 +بعمل destruction لمواد غذائية مطلوبة لنمو + +296 +00:21:12,160 --> 00:21:12,780 +البكتيريا + +297 +00:21:20,030 --> 00:21:22,390 +بيحتاجوا على نفسي المكونات تبع ال blood agar مع + +298 +00:21:22,390 --> 00:21:28,510 +اختلافات طفيفة اللى سببها اللى هو التسخين بالحرارة + +299 +00:21:28,510 --> 00:21:31,350 +صار عندي بعض المواد متاحة فى الوسط ماكانتش متاحة + +300 +00:21:31,350 --> 00:21:36,170 +صار عندي مواد صارها inhibition رميرات وصار فى عندي + +301 +00:21:36,170 --> 00:21:40,690 +مواد ايضا خسرناها مواد غذائية الان السؤال اللى + +302 +00:21:40,690 --> 00:21:44,190 +بيطرح نفسه كمان مرة مين أحسن ال blood agar و لا ال + +303 +00:21:44,190 --> 00:21:49,380 +chocolate agar حسب البلد اللى ممكن عشان بتحتاجفي + +304 +00:21:49,380 --> 00:21:52,440 +ناس بدأت تتعلم و تقول انه مافيش امكانية على إيجاب + +305 +00:21:52,440 --> 00:21:56,080 +هذا السؤال بأبيض و أسود، إن هو أحسن من هذا، في + +306 +00:21:56,080 --> 00:22:00,840 +عندنا استخدام و استخدام آخر، بمعنى إن الـ + +307 +00:22:00,840 --> 00:22:03,000 +chocolate agar له استخدامات و ال butter agar له + +308 +00:22:03,000 --> 00:22:07,900 +استخدامات أخرى، ليش ذكرنا ال chocolate agar في + +309 +00:22:07,900 --> 00:22:12,340 +النيسيريا؟ النيسيريا جنورية بالذات is very fragile + +310 +00:22:12,340 --> 00:22:17,590 +organism، إيش يعني fragile؟ إيش يعني هشة؟يعني أي + +311 +00:22:17,590 --> 00:22:23,290 +إشي بيأثر عليها ممكن تموت ايش يعني أي إشي؟ عارفين + +312 +00:22:23,290 --> 00:22:27,490 +درجة حرارة 38 ممكن تقتلها؟ درجة حرارة 38 ممكن + +313 +00:22:27,490 --> 00:22:33,930 +تقتلها لو حطينا كحول على مكان الإصابة، تمام؟ و .. + +314 +00:22:33,930 --> 00:22:37,510 +و ده الجزء الفسيد جدا من الكحول، ممكن تموت لو + +315 +00:22:37,510 --> 00:22:39,610 +حطيناها فوق تلك الكحولة، بعد ما نخلص من شغل + +316 +00:22:39,610 --> 00:22:42,090 +البكتيرا، و ايه بحطها؟ فوق ال incubator، جنب ال + +317 +00:22:42,090 --> 00:22:45,400 +incubator في علبة، لعلينا نتخلص من ال autoclaveلو + +318 +00:22:45,400 --> 00:22:48,740 +قعدت يوم فوق ال incubator أو يومين بتموت بينما ال + +319 +00:22:48,740 --> 00:22:50,840 +E.coli لو بعد أسبوعين لاجيناها بيبقى بيبقى بيبقى + +320 +00:22:50,840 --> 00:22:52,060 +بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى + +321 +00:22:52,060 --> 00:22:52,460 +بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى + +322 +00:22:52,460 --> 00:22:52,920 +بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى + +323 +00:22:52,920 --> 00:23:00,460 +بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى بيبقى + +324 +00:23:00,460 --> 00:23:12,200 +بيبقى بيبقى + +325 +00:23:12,200 --> 00:23:18,020 +بيأحنا في بعض البكتيريا ومنها الناسيريا جونورية + +326 +00:23:18,020 --> 00:23:24,100 +بتحتاج CO2 فكيف بنوفرنا إياها؟ بنجيب إناء مغلط + +327 +00:23:24,100 --> 00:23:27,680 +محكم مغلط بنفتحه بنحط البكتيريا اللي الأطباق اللي + +328 +00:23:27,680 --> 00:23:31,560 +بنزرعها أو زرعناها و بنجيب شريحة و بنلزج عليها + +329 +00:23:31,560 --> 00:23:35,300 +شامعة و بنولع الشامعة و بننزلها في ال .. في الإناء + +330 +00:23:35,300 --> 00:23:39,320 +و بنسكن الان الشامعة هتستهلك معظم ال .. او مانيش + +331 +00:23:39,320 --> 00:23:42,520 +أقول معظم كمية كبيرة من ال oxygen و بعدين تنطفى + +332 +00:23:42,520 --> 00:23:46,850 +تستهلك ال oxygenأو جزء من الأكسجين و بتطلب منها + +333 +00:23:46,850 --> 00:23:51,330 +CO2 فبتوفر انهم يسموها CO2 jar تقنيك مشهور هتعلمه + +334 +00:23:51,330 --> 00:23:57,190 +في المختبر ان شاء الله واحد طلاب خلص الشميع فاخدنا + +335 +00:23:57,190 --> 00:24:00,910 +شميع تاني من نوع تاني قعدنا أربع خمس أيام ولا + +336 +00:24:00,910 --> 00:24:04,110 +عزلنا ناسيه رياجودرية كنا نازلها في اليوم ست سبع + +337 +00:24:04,110 --> 00:24:08,190 +مرات ناست سبع أينات يعني نازلها في الفترة اللي + +338 +00:24:08,190 --> 00:24:13,630 +غيرنا فيها الشميع zero isolates negativeتلافنا ان + +339 +00:24:13,630 --> 00:24:16,910 +الشامل اللي استخدمناه هو شامل أحمر و الشامل الأحمر + +340 +00:24:16,910 --> 00:24:20,630 +هو عبارة عن صابغة و الصابغة fumes بتطلع مع الحرق + +341 +00:24:20,630 --> 00:24:25,330 +تمام فمن كتر ملبكتيريا حساسة لم تنمو ولا واظر + +342 +00:24:25,330 --> 00:24:29,610 +عملها دلوقتي فإن سيريا جونوريا is very fragile + +343 +00:24:29,610 --> 00:24:33,870 +very sensitive فبتتعامدها وساط تكون خالي من ال + +344 +00:24:33,870 --> 00:24:37,130 +inhibitors فى وقت بتاع اسمه مضطر مارتن أجار هو + +345 +00:24:37,130 --> 00:24:42,110 +selective media يشبه في تركيبه إلى حد كبير ال + +346 +00:24:42,110 --> 00:24:44,950 +chocolate agarلكن يضاف إليها تلات أنواع من ال + +347 +00:24:44,950 --> 00:24:50,170 +antibiotics لازم أكتبلكوا إياهم عشان نعرفه ال + +348 +00:24:50,170 --> 00:24:55,470 +antibiotics بيختصرونهم هو بيختصروا بواسطة Tm اللي + +349 +00:24:55,470 --> 00:25:01,730 +هو ثانية المارة نجار Vcn ال Vcn هدولها تلات أحرف + +350 +00:25:01,730 --> 00:25:06,530 +هم تلات أسماء لتلات انتبايتك Vcn + +351 +00:25:20,280 --> 00:25:28,340 +vancomycin كوليستين نستاتين + +352 +00:25:28,340 --> 00:25:32,100 +كتبرهم ولا واضحين؟ + +353 +00:25:56,790 --> 00:26:02,610 +Vancomycin Cوليستين ونستاتين طبعا ال Vancomycin + +354 +00:26:02,610 --> 00:26:08,850 +نستخدمه لمنع الجرام positive بكتيريا الكوليستين + +355 +00:26:08,850 --> 00:26:15,930 +نستخدمه لمنع الجرام negative بكتيريا النستاتين + +356 +00:26:15,930 --> 00:26:18,010 +نستخدمه لمنع الفطريات + +357 +00:26:41,590 --> 00:26:46,430 +طبعا هنا بيكونوا يعرفوا انه ال genital tract + +358 +00:26:46,430 --> 00:26:52,090 +infection و ال urinary بيسميه genitourinary tract + +359 +00:26:52,090 --> 00:26:57,880 +يعني في اتصال بينهمفلو المرض عنده Infection بجرام + +360 +00:26:57,880 --> 00:27:01,040 +نيجاتف زي الاشريشيا كولايه هتعمل over grow على + +361 +00:27:01,040 --> 00:27:05,830 +الناس هنالو عندها enterococcus، المريض عنده + +362 +00:27:05,830 --> 00:27:09,110 +enterococcus، هتعمل overgrow على الناسيريا، لو عند + +363 +00:27:09,110 --> 00:27:11,930 +المريض infection بيكانديا albicans، برضه هعمل + +364 +00:27:11,930 --> 00:27:16,570 +overgrow على اللي هو ال .. ال .. ال .. ال .. + +365 +00:27:16,570 --> 00:27:19,130 +الناسيريا، وبالتالي احنا حاطينا ال Antibiotics + +366 +00:27:19,130 --> 00:27:24,050 +تقوله على أساس انه نمنع نمو هذه البكتيريا، + +367 +00:27:24,050 --> 00:27:28,190 +وبالتالي نسمح للناسيريا gonorrhea، لإن كمان مرة هي + +368 +00:27:28,190 --> 00:27:32,070 +حساسة وماعناش إمكانية للمنافسة مع بكتيريا أخرى + +369 +00:27:44,710 --> 00:27:47,450 +هو المشكلة أن هذا الميكروب ممكن تموت لو كان في + +370 +00:27:47,450 --> 00:27:50,510 +أماكن بيصل في هذا الحرارة كل ما عن ال genitorial + +371 +00:27:50,510 --> 00:27:53,610 +tract غالب ال external genitalia ده ال male و ال + +372 +00:27:53,610 --> 00:27:56,570 +female درجة حرارتها بيكون أقل من درجة حرارة الجسم + +373 +00:27:56,570 --> 00:27:59,810 +فحتى لو ارتفعت سبعة و تلاتين و تسعة و تلاتين و + +374 +00:27:59,810 --> 00:28:01,750 +تسعة و تلاتين و تسعة و تلاتين ممكن ما تأثرش على ال + +375 +00:28:01,750 --> 00:28:02,710 +.. ال .. ال .. ال .. ال + +376 +00:28:05,730 --> 00:28:08,710 +بس للتمييز بين نيسيريا جانورية و نيسيريا مينينجيتس + +377 +00:28:08,710 --> 00:28:11,990 +فى عندنا بعض ال virulence factors they have + +378 +00:28:11,990 --> 00:28:15,690 +similar virulence factors شوفوا ال نيسيريا + +379 +00:28:15,690 --> 00:28:18,190 +مينينجيتس احنا مركزينه على ال ليبو بوليساكرايد + +380 +00:28:18,190 --> 00:28:21,670 +مضخمينه ال ليبو بوليساكرايد و ال capsule و لأن + +381 +00:28:21,670 --> 00:28:26,830 +هلولا هم اهم عاملين للبكتيريا لنيسيريا مينينجيتس + +382 +00:28:26,830 --> 00:28:30,230 +تمام اللى بيعمله infection و بيقالولها تعمل + +383 +00:28:30,230 --> 00:28:34,370 +meningitis لكن عندها برضه مجموعة من الحوامل زى + +384 +00:28:34,370 --> 00:28:34,870 +اللى همولسين + +385 +00:28:39,920 --> 00:28:42,300 +هتعرف على إيش هتنقل بس تبروتين و أكتر من برا + +386 +00:28:42,300 --> 00:28:45,660 +بروتين كمان شوية على جهة تانية المقابلة أبرزنا + +387 +00:28:45,660 --> 00:28:49,760 +مين؟ أبرزنا ال بالاي أكتر حاجة بس أنا .. أنا .. + +388 +00:28:49,760 --> 00:28:53,020 +أنا لخاطر أكمل كمان ال IGA Protease IGA Protease + +389 +00:28:53,020 --> 00:28:56,700 +كتير مهم في ده الموضوع بس ال بالاي بالفعل هي + +390 +00:28:56,700 --> 00:29:01,240 +الليها ماعندهاش capsule ماعندهاش hemolysin بينما + +391 +00:29:01,240 --> 00:29:03,380 +ان Syria mainly likes عندها capsule + +392 +00:29:06,920 --> 00:29:10,100 +ناسيريا جونورية و بندللها بالاسم الكشائع عليها + +393 +00:29:10,100 --> 00:29:14,120 +Gonococcus الناسيريا ال mainly طيبة اسم اللي + +394 +00:29:14,120 --> 00:29:17,680 +بندللها هو هنا مينينجو كوكس الستريبتو كوكس + +395 +00:29:17,680 --> 00:29:23,620 +نيومونيا هي تلاتة صارو كوكس مينينجو و جونو كوكس و + +396 +00:29:23,620 --> 00:29:27,560 +نيومو كوكس أخدنا الشق الأول منه ال species و + +397 +00:29:27,560 --> 00:29:31,960 +أعطينا كلمة كوكس مينينجو كوكس نيومو كوكس و جونو + +398 +00:29:31,960 --> 00:29:35,620 +كوكس ال incubation period من اتنين لاربع اخر يوم + +399 +00:29:36,240 --> 00:29:39,180 +فهو بيقوللي إن مانو مانو مقصود فيها ناشر الإنسان + +400 +00:29:39,180 --> 00:29:43,500 +ليس مقصود فيها الرجل وانما الإنسان هي بتصيب البكر + +401 +00:29:43,500 --> 00:29:50,200 +و الأنفة الجملة جملة كتير مهمة is the second most + +402 +00:29:50,200 --> 00:29:53,400 +common venereal disease كلمة venereal STD sexually + +403 +00:29:53,400 --> 00:29:56,480 +transmitted disease sexually transmitted disease + +404 +00:29:56,480 --> 00:30:00,600 +كلمة + +405 +00:30:00,600 --> 00:30:07,070 +second most common يعني تتلوأو تتبع ال number one + +406 +00:30:07,070 --> 00:30:11,090 +من ال number one اللي هي ال chlamydia ال chlamydia + +407 +00:30:11,090 --> 00:30:16,510 +مشهورة بتسبب أسترية بشكل كبير تلعب الصورة اللي + +408 +00:30:16,510 --> 00:30:19,950 +قدامكوا هذه الصورة اللي قدامنا هنا مأخوذة واحدة + +409 +00:30:19,950 --> 00:30:24,310 +منهم من ال pure culture ومكبرة أكتر منها تلعب دبله + +410 +00:30:24,310 --> 00:30:30,950 +cocaine واحد اتنين واحد اتنين واحد اتنين وفي في + +411 +00:30:30,950 --> 00:30:36,400 +الصورة التانية إذا بتلاحظوافي عندي نيتروفيل، + +412 +00:30:36,400 --> 00:30:40,560 +النيتروفيل فيها نوكلياس و مليانة دبلوكوكاية، + +413 +00:30:40,560 --> 00:30:47,480 +مليانة دبلوكوكاية، هي واحدة كل هذه و هذه لكن كمان + +414 +00:30:47,480 --> 00:30:52,960 +برضه بلاقي دبلوكوكاية الموجودة في المحيط فبنجي + +415 +00:30:52,960 --> 00:30:56,640 +نقول عنها انه exists intracellular and + +416 +00:30:56,640 --> 00:30:58,160 +extracellular + +417 +00:31:00,450 --> 00:31:04,310 +it can exist, it can grow freely وده يعني ده جانب + +418 +00:31:04,310 --> 00:31:07,890 +اللي بنميها في المختبر بطريقة ال .. ال plates هذا + +419 +00:31:07,890 --> 00:31:11,050 +المعيشة عنها بتنمو إيه؟ freely، لو انها obligate + +420 +00:31:11,050 --> 00:31:13,610 +intracellular بنات مابنجرش نميها إلا على cell + +421 +00:31:13,610 --> 00:31:18,450 +culture بس لما بيقول facultative intracellular + +422 +00:31:18,450 --> 00:31:24,380 +بتجي تنمو، هانو تنمو على داخل الخلاياالمصطلح هو + +423 +00:31:24,380 --> 00:31:28,320 +polymorphonuclear cells ايه كانت polymorphonuclear + +424 +00:31:28,320 --> 00:31:31,880 +cells؟ و بيختصروها P M N S P M N S + +425 +00:31:31,880 --> 00:31:37,040 +polymorphonuclear cells هي نفسها بقية النيتروفيل + +426 +00:31:37,040 --> 00:31:40,740 +هي نفسها الفرغوسايتز يعني اللي بتكلم على P M N S + +427 +00:31:40,740 --> 00:31:44,440 +او بتكلم عن نيتروفيل او فرغوسايتز بتكلم عن نفس + +428 +00:31:44,440 --> 00:31:48,440 +الخط نفس + +429 +00:31:48,440 --> 00:31:53,060 +الصورةطيب نيجي ل ال .. ال pilli و ال proteins + +430 +00:31:53,060 --> 00:31:58,040 +pilli is important in adhering اعتقد انه حكيناها + +431 +00:31:58,040 --> 00:32:00,740 +اوكي لما .. لما كنا نتكلم عن ال variants factor في + +432 +00:32:00,740 --> 00:32:05,220 +كل عام لأ ذكرت انا ان النقطة كتير مهمة انه ال + +433 +00:32:05,220 --> 00:32:09,340 +pilli أهميتها للنسية الجنودية تختلف عن كل .. و + +434 +00:32:09,340 --> 00:32:12,380 +لذلك أبرزناها احنا بخط كبير في اللوحة لما عملنا + +435 +00:32:12,380 --> 00:32:18,130 +مقارنة بين التانيةما هي ميزتها؟ إن الرسم تحرف + +436 +00:32:18,130 --> 00:32:22,070 +أهمية البيلاي فكوّن عيساها مضادة، هي عرفت إن الرسم + +437 +00:32:22,070 --> 00:32:28,010 +فصارت عاملة genetic variation أو عفوًا gene + +438 +00:32:28,010 --> 00:32:31,570 +variation وبالتالي صارت تدخلنا برضه مرار وتكرار + +439 +00:32:31,570 --> 00:32:37,410 +بأشكال متنوعة من ال antigen تبع البيلاي تخيلوا كده + +440 +00:32:37,410 --> 00:32:43,470 +أهميتها non-pileated strains أو mutants are aviral + +441 +00:32:45,010 --> 00:32:47,210 +اللي مالهاش كابسول تبعت لسترتو كوكا سنيمونيا اللي + +442 +00:32:47,210 --> 00:32:52,170 +مالهاش كابسول مالهاش pathogenesis فاللي مالهاش + +443 +00:32:52,170 --> 00:32:57,170 +بيلايهانا مالهاش أي pathogenesis من الأوتر + +444 +00:32:57,170 --> 00:33:00,670 +proteins في عندنا protein واحد و protein اتنين و + +445 +00:33:00,670 --> 00:33:04,270 +protein تلاتة لكن كل واحد منهم له اسم أخر غير عن + +446 +00:33:04,270 --> 00:33:09,050 +الرقم المعطير فمثلا part of protein اللي بيسموه + +447 +00:33:09,050 --> 00:33:12,250 +protein واحد prevent phagolizosome fusion اظن ان + +448 +00:33:12,250 --> 00:33:16,900 +كلكوا فاكرين الفاجوزومالفاجوزوم اللى جواته + +449 +00:33:16,900 --> 00:33:22,200 +الفكتيريا تمام؟ والليزوزوم اللى بيجي بيتحد معاه + +450 +00:33:22,200 --> 00:33:26,880 +الآن العملية الضرورية فى ال normal phagocytosis + +451 +00:33:26,880 --> 00:33:31,040 +حددت ال porn protein بيمنع الالتحام ما بين + +452 +00:33:31,040 --> 00:33:37,000 +الفاجوزوم والليزوزوم بيكونش فاجوليزوزوم وبالتالي + +453 +00:33:37,000 --> 00:33:40,560 +بيبقى وانا اللى بيخلي الفكتيريا قادرة على انها + +454 +00:33:40,560 --> 00:33:41,940 +تعمل intracellular + +455 +00:33:44,120 --> 00:33:49,140 +وبست بروتين وهذا بيساعد البكتيريا بشكل جيد جدا على + +456 +00:33:49,140 --> 00:33:52,240 +ال binding يعني هو أبدا عن attachment أبدا عن + +457 +00:33:52,240 --> 00:33:56,180 +attachment في كمان هاسمها RMB او reduction + +458 +00:33:56,180 --> 00:33:59,480 +modifiable protein وهذا بيعمل عملية protection + +459 +00:33:59,480 --> 00:34:04,500 +للبكتيريا من ال bactericidal antibodies اذا على + +460 +00:34:04,500 --> 00:34:10,500 +الرغم من انها حساسة الا انها مزودة يعني بعن اخر هي + +461 +00:34:10,500 --> 00:34:18,070 +مصممةdesigned ولمّا قلت only for man موجودة فقط في + +462 +00:34:18,070 --> 00:34:22,830 +الإنسان ولا تنتقل بطرق أخرى إلا عبر الجنس إذا لمن + +463 +00:34:22,830 --> 00:34:27,350 +يمارس هنا الرديلة هي مخصصة ومصممة لهم انت هنا يعني + +464 +00:34:27,350 --> 00:34:33,290 +حتى يعني طبعا إمكانية الهياجت موجودة لكن لاحظته في + +465 +00:34:33,290 --> 00:34:39,990 +ال immunity انه انعطم عضنا لأنه في بإمكانية تغيير + +466 +00:34:39,990 --> 00:34:46,380 +ل اللي هو ال بالعيبمقدرتها على مكافحات ال .. ال .. + +467 +00:34:46,380 --> 00:34:50,420 +ال .. ال antibiotics عالي جدا، يعني أنا أذكر في .. + +468 +00:34:50,420 --> 00:34:56,210 +يمكن قبل عشرين سنة قرأت مقالة عن Hong KongHong + +469 +00:34:56,210 --> 00:35:00,730 +Kong ونسبة ال resistance لل نيسيريا الجنورية في + +470 +00:35:00,730 --> 00:35:05,150 +Hong Kong كانت النسبة أكتر من 95% resistant لل + +471 +00:35:05,150 --> 00:35:07,470 +Penicillin اللي كان drug of choice كان drug of + +472 +00:35:07,470 --> 00:35:10,410 +choice في يوم الأيام وبالتالي صحيح هي delicate + +473 +00:35:10,410 --> 00:35:15,970 +outside حتى لا تنتقل للناس العاديين تمام؟ اللي + +474 +00:35:15,970 --> 00:35:20,250 +مالومش دعوة وبالتالي تقولنا كده مرة واضح إنها + +475 +00:35:20,250 --> 00:35:23,390 +specifically designed يعني مصممة لهذا الغرض + +476 +00:35:26,420 --> 00:35:28,840 +طبعا انا لم .. انا لم .. انا لم .. يعني اجروا ان + +477 +00:35:28,840 --> 00:35:32,040 +احطلكوا صور للنسية الجانورية لان كلها بقعد + +478 +00:35:32,040 --> 00:35:35,180 +external genitalia كلها و العرض الوحيد اللي هو + +479 +00:35:35,180 --> 00:35:41,040 +عملية ال discharge اللي هي الافرازات الصدرية اللي + +480 +00:35:41,040 --> 00:35:44,520 +بتخرج من ال .. ال female organ او ال male organ + +481 +00:35:44,520 --> 00:35:50,640 +لكن دي بتصورة بتوضح الضحية اللي بيولد لأم مصابة ب + +482 +00:35:50,640 --> 00:35:55,010 +.. اللي هو ال .. ال .. الجانوريةخلال .. اظن اننا + +483 +00:35:55,010 --> 00:35:59,310 +حكينا عن قناة الولادة ان الطفل بياخد معاه ميكروبات + +484 +00:35:59,310 --> 00:36:03,330 +وهذه الميكروبات الاصل انها كويسة .. كويسة اقولكوا + +485 +00:36:03,330 --> 00:36:07,190 +بدونها .. بدونها يعني الطفل .. فاكريني لما مرة + +486 +00:36:07,190 --> 00:36:09,890 +ناقشنا قولنا انه الأطفال اللي عندهم .. بناخدهم + +487 +00:36:09,890 --> 00:36:13,330 +سيزيريا أكتر عرضة ممكن تكون السبب انهم ماناخدوش + +488 +00:36:13,330 --> 00:36:16,150 +عبر قناة الولادة ومااخدوش منه ال M normal + +489 +00:36:16,150 --> 00:36:19,910 +microbiota لكن لو يكون فيه pathogens بعض الطفل + +490 +00:36:19,910 --> 00:36:23,930 +بياخد pathogenوفي هذه الحالة هو جهاز الملاعب لم + +491 +00:36:23,930 --> 00:36:27,670 +يكتمل بعد انه ال flora تبعه لم تكتمل بعد وبالتالي + +492 +00:36:27,670 --> 00:36:30,710 +قد يصاب باتهابات في العين بنسميها ophthalmia + +493 +00:36:30,710 --> 00:36:34,450 +neonatrum ولاحظوا الصديد اللي طالع من العين كميات + +494 +00:36:34,450 --> 00:36:39,250 +بخمة من الصديد نفس اللون الصديد هذا هو اللي بنشوفه + +495 +00:36:39,250 --> 00:36:44,690 +خارج من ال male أو ال female genital area أو organ + +496 +00:36:44,690 --> 00:36:48,930 +طبعا ال ophthalmia neonatrum condition مابسبوش + +497 +00:36:48,930 --> 00:36:52,420 +بكتيريا واحدة بسبب مجموعة من ال micro organismمن + +498 +00:36:52,420 --> 00:36:55,280 +ضمنها اللي هي .. ماعرف شو هي الواضحة دي لأ صبورة + +499 +00:36:55,280 --> 00:36:58,200 +اللي هي ال clamydia اللي هي جناها نجو شوية number + +500 +00:36:58,200 --> 00:37:05,460 +one cause of asterese او venereal disease ال + +501 +00:37:05,460 --> 00:37:08,920 +gram stain it is poly .. it can be found in + +502 +00:37:08,920 --> 00:37:11,240 +polymorph vascular cells شفتوا نجو شوية في ال + +503 +00:37:11,240 --> 00:37:14,750 +nitrophil صح؟gram negative coccyte either + +504 +00:37:14,750 --> 00:37:17,910 +intracellular or extracellular either يعني ممكن + +505 +00:37:17,910 --> 00:37:21,310 +نجيها الشريحة الواحدة اللي جيها في التانين اللي + +506 +00:37:21,310 --> 00:37:25,810 +جيها intracellular و extracellular الآن هنا النقطة + +507 +00:37:25,810 --> 00:37:29,050 +أذكركم الأن أخدنا grams 10 من أنثى و أخدنا grams + +508 +00:37:29,050 --> 00:37:32,790 +10 من ذكر اختلف الوضع صح؟ إذا ولدنا gram negative + +509 +00:37:32,790 --> 00:37:35,930 +دبله كوكاية في الذكر لإن احنا بنعرف إنه ماعندوش + +510 +00:37:35,930 --> 00:37:38,590 +نيسيريا normal flora فعنده نيسيريا الجيورية + +511 +00:37:38,590 --> 00:37:41,450 +شخصناهالكن لو أخدناها من أنثا ولا جانا عندها + +512 +00:37:41,450 --> 00:37:44,930 +جراميه تدبل الكوكاية، يمكنها تكون normal flora، + +513 +00:37:44,930 --> 00:37:49,890 +لذلك أصلا في المختبرات يرفض استقبال urethral + +514 +00:37:49,890 --> 00:37:53,990 +discharge كجراميستين لنيسيريا جنورية لما تكون عينا + +515 +00:37:53,990 --> 00:37:59,180 +من 100 من أنثا، لأنها uselessفقط في ال culture + +516 +00:37:59,180 --> 00:38:02,980 +طبعا لأ أصلا يعني مقتنعش على الإجرام الستاني في + +517 +00:38:02,980 --> 00:38:06,480 +التشخيص النهائي ولكن كتشخيص مبدأ إيه؟ في الدكار + +518 +00:38:06,480 --> 00:38:09,020 +ممكن نقول إنه عندنا إي سيريا جنورية لكن في الوقت + +519 +00:38:09,020 --> 00:38:11,980 +لأ ما أستطيع إلا على طريق اللي هو وطريقة اللي هو + +520 +00:38:11,980 --> 00:38:14,920 +أصلا في الآخر culture على طير مارتن أجاروت أنت + +521 +00:38:14,920 --> 00:38:21,520 +بايتكس فاستهما لسه ال VCN-Vancomycin-Cholestin + +522 +00:38:21,520 --> 00:38:26,980 +-Nestatine لنطلع وحفظينهم يعني ويمكن نميح على ال + +523 +00:38:26,980 --> 00:38:33,020 +chocolate أجارأنا ذكرتلكوا خلال المحاضرة أنها + +524 +00:38:33,020 --> 00:38:40,640 +تتطلب الـ CO2 صح؟ و الـ CO2 بيكون فيه جار خاص + +525 +00:38:40,640 --> 00:38:45,300 +يسميها CO2 جار خلينا نربط معلومة بمعلومة و نروح + +526 +00:38:45,300 --> 00:38:49,060 +لمختبر كل الميكروبات + +527 +00:38:51,680 --> 00:38:54,840 +لأن في مشروطين كلمة مشروطين يعني بدها special + +528 +00:38:54,840 --> 00:38:57,680 +environments أو special conditions زي البروسيلة + +529 +00:38:57,680 --> 00:39:01,740 +مثلا واحدة ست أسابيع بدها مش ممكن أزرعها مع + +530 +00:39:01,740 --> 00:39:04,740 +بكتيريا تانية هاتولي طبق ممكن يصمم ست أسابيع من + +531 +00:39:04,740 --> 00:39:07,400 +غير ما ينشف في ال incubator مافيش إمكانية + +532 +00:39:07,400 --> 00:39:10,960 +فالبروسيلة بنزرعها بطريقة مختلفة تماما عن ال other + +533 +00:39:10,960 --> 00:39:15,600 +samples كل بكتيريا الروتينية اللي بتحتاج CO2 + +534 +00:39:15,600 --> 00:39:19,180 +بنزرعها على تشكرة أجار حتى وإن كانت تنمو على برض + +535 +00:39:19,180 --> 00:39:22,760 +أجارما هو اللى بتنمو على أجار اللى هتنمو على مين؟ + +536 +00:39:22,760 --> 00:39:25,940 +على ال chocolate أجار فبنزرعها و بنميها على ال + +537 +00:39:25,940 --> 00:39:28,560 +chocolate أجار و بنحطها في ال CO2 إذا غالبا في + +538 +00:39:28,560 --> 00:39:32,440 +المعمل هتكون عندكوا طبق اسمه ال chocolate أجار وين + +539 +00:39:32,440 --> 00:39:37,800 +بتتم زراعته أو وين بتتم تحضينه عفوا under CO2 + +540 +00:39:37,800 --> 00:39:42,160 +conditions طبعا هنا فيه أنظمة CO2 incubation + +541 +00:39:44,710 --> 00:39:50,170 +هو مختبر الوايكل بتشوفه فيه نان انكبيتر فور بعض + +542 +00:39:50,170 --> 00:39:56,930 +التحتاني هذا الـCO2 الكبير هذا الـCO2 انكبيتر هو + +543 +00:39:56,930 --> 00:40:01,150 +عبارة عن انكبيتر عادي موصول باستوارة بتدخله CO2 + +544 +00:40:01,150 --> 00:40:07,130 +بنسبة وفي دي water sensor إذا قلت النسبة بيشغل إذا + +545 +00:40:07,130 --> 00:40:09,110 +زالت النسبة بيوقف وانتهى الموضوع + +546 +00:40:15,080 --> 00:40:18,060 +Antibiotic therapy أنا قلتلك البكتيريا يعني من + +547 +00:40:18,060 --> 00:40:22,180 +الأنواع اللي أصبحت مقاومة لمين للبنسيلينات لذلك + +548 +00:40:22,180 --> 00:40:26,480 +بنلاجئ إنه بنستخدملها antibiotic اسمه ceftriaxone + +549 +00:40:26,480 --> 00:40:29,800 +وceftriaxone يتبع مجموعة اسمة cephalosporin + +550 +00:40:29,800 --> 00:40:36,300 +وcephalosporin هي عبارة عن drugs أو antibiotics في + +551 +00:40:36,300 --> 00:40:39,120 +جزء منها مقاوم لل Beta-lactamase إيش ال Beta + +552 +00:40:39,120 --> 00:40:39,840 +-lactamase؟ + +553 +00:40:42,170 --> 00:40:45,150 +اللي تنتج البكتيريا عشان تكسر ال beta-lactam + +554 +00:40:45,150 --> 00:40:48,170 +antibiotic تدخل البنسالين في ال beta-lactam + +555 +00:40:48,170 --> 00:40:53,210 +antibiotic البنسالين و ال cephalosporins كيميائيا + +556 +00:40:53,210 --> 00:40:57,270 +بيحتووا على ما يسمى beta-lactam ring beta-lactam + +557 +00:40:57,270 --> 00:41:03,730 +ring نايت من أول بنسالين و cephalosporins بيحتووا + +558 +00:41:03,730 --> 00:41:07,850 +على beta-lactam ring كمكون أساسي وبتم تصنيفهم على + +559 +00:41:07,850 --> 00:41:11,030 +ايه beta-lactam antibiotics beta-lactam + +560 +00:41:11,030 --> 00:41:14,020 +antibioticsChemically they are different هذا + +561 +00:41:14,020 --> 00:41:16,780 +penicillins و هذا cephalosporins حتى ال + +562 +00:41:16,780 --> 00:41:20,680 +cephalosporins في أصناع و أنواع كتيرة منها و كانت + +563 +00:41:20,680 --> 00:41:24,440 +الأولى بالنسبة لميه؟ البنسالينال البكتيريا بتقاوم + +564 +00:41:24,440 --> 00:41:28,640 +أنتبيتك ممكن تقاومه عن طريق إنتاج إنزيم يكسر + +565 +00:41:28,640 --> 00:41:33,160 +الانتبيت فال beta لك تميز لحظة بالجامعة + +566 +00:41:35,300 --> 00:41:39,300 +وهي مش مولودة هنا Beta-lactamases لما بتتكلم عن + +567 +00:41:39,300 --> 00:41:41,980 +أنزام أنزام تقول Beta-lactamases يعني بقى بتحكي + +568 +00:41:41,980 --> 00:41:47,080 +إنه هي more than one Beta-lactam enzyme فهناك + +569 +00:41:47,080 --> 00:41:50,980 +Antibiotic تتأثر بال Beta-lactamases و بتصير + +570 +00:41:50,980 --> 00:41:55,920 +ineffective فالبكتيريا بتصير الآن مقاومة عشان أقدر + +571 +00:41:55,920 --> 00:41:59,840 +أقتل البكتيريا هذه لازم أستخدم Sepharosporin أو + +572 +00:41:59,840 --> 00:42:03,540 +Penicillin مقاوم لل Beta-lactamase تبع البكتيريا + +573 +00:42:04,260 --> 00:42:06,660 +يبقى جيش اسمه الـ beta-lactamase resistant + +574 +00:42:06,660 --> 00:42:11,080 +cephalosporin مقاوم لمين؟ للـ beta-lactamase اللى + +575 +00:42:11,080 --> 00:42:14,640 +هى تستخدمه لمقاومة اللى هو الـ beta-lactam + +576 +00:42:14,640 --> 00:42:18,120 +antibiotic مثل علي safe reaction قبل تكون الصورة + +577 +00:42:18,120 --> 00:42:24,600 +واضحة انه هناك بعض ال antibiotic بدأت تتأثر سلبا + +578 +00:42:24,600 --> 00:42:27,920 +بإنزيمة تتكسر يعنى بفعل إنزيمة اسمها beta + +579 +00:42:27,920 --> 00:42:30,890 +-lactamasesعشان اقدر اعالج انا بكتيريا بيها لازم + +580 +00:42:30,890 --> 00:42:35,070 +يكون عندي مقاوم لهادي ال beta-lactam او ال beta + +581 +00:42:35,070 --> 00:42:39,430 +-lactamase ال resistance strains common they + +582 +00:42:39,430 --> 00:42:42,890 +produce beta-lactamases زي ما ذكرت beta-lactamases + +583 +00:42:42,890 --> 00:42:49,490 +map and destroy penicillin الميكروب التاني في + +584 +00:42:49,490 --> 00:42:52,530 +السلسلة اللي هو ناسيريا meningitis او meningitis + +585 +00:42:52,530 --> 00:42:58,010 +او اللي زي ما قلنا بندلع meaning و caucus وشكل + +586 +00:42:58,010 --> 00:42:58,470 +كتير حلو + +587 +00:43:05,570 --> 00:43:11,490 +بالتسجيل هتشوفوها واضحة لإن بيصوروها من الجهاز + +588 +00:43:11,490 --> 00:43:16,170 +نيسيريا مينيديز او منيديز man is the only natural + +589 +00:43:16,170 --> 00:43:21,370 +known host برضه الإنسان هو ال host الطبيعي المعروف + +590 +00:43:21,370 --> 00:43:24,710 +usually sporadic cases may cause epidemics + +591 +00:43:30,700 --> 00:43:37,700 +epidemics يعني بشكل اوبيا يعني انتشار اكتر من + +592 +00:43:37,700 --> 00:43:43,880 +المعهود mostly young children هم الستة و ال + +593 +00:43:43,880 --> 00:43:48,320 +population لكن ال outbreaks ممكن تحدث لل adults و + +594 +00:43:48,320 --> 00:43:52,140 +خاصة في ال crowded conditions مثلا army براكس + +595 +00:43:52,140 --> 00:43:56,300 +براكسات للجنود خاصة اللي بيجيبوهم جداد يسموهم + +596 +00:43:56,300 --> 00:44:01,150 +recruits المجندين الجدد المجندين الجددبره في بعض + +597 +00:44:01,150 --> 00:44:04,270 +الجامعات او هتفضل عربية في ما يسمى بسكن الطلبة، + +598 +00:44:04,270 --> 00:44:08,250 +سكان الطلبة، برضه الطلبة الجدد اللي بيجي بيسكنوا + +599 +00:44:08,250 --> 00:44:11,890 +في الجامعات و ممكن يسكنهم out in breaks طيب خلينا + +600 +00:44:11,890 --> 00:44:16,150 +نستغل الفرصة و نتكلم عن Nasirian meningitis من + +601 +00:44:16,150 --> 00:44:22,750 +خلال ال army barracks و ال college students انتوا + +602 +00:44:22,750 --> 00:44:27,450 +كلكوا سكان قطاع غز، ماحدة جاي من برا؟لمّا احنا + +603 +00:44:27,450 --> 00:44:32,510 +طلعنا برا ندرس طبعا، طلعنا كان فيه من الأردن و من + +604 +00:44:32,510 --> 00:44:35,950 +سوريا و من العراق، من دول مختلفة و جايين كلنا كلنا + +605 +00:44:35,950 --> 00:44:40,900 +على دول اسمها الفلبي، قعدنا ندرس فيهاعارفين اللي + +606 +00:44:40,900 --> 00:44:44,280 +سافر اول اللي .. اللي .. اللي بسافر و هو صغير خاصة + +607 +00:44:44,280 --> 00:44:47,460 +في حالة يسموها home sickness home sickness يعني + +608 +00:44:47,460 --> 00:44:51,680 +بيصير حن للوطن بعد ما كان بده يعني بأي وسيلة يطلع + +609 +00:44:51,680 --> 00:44:54,920 +بيصير حن و ممكن يتكلم على هذا الأوضة و يبقى بيبكي + +610 +00:44:54,920 --> 00:45:00,460 +يعني عشان هو غادر ال .. ال .. الأحد دولة فبيشعر + +611 +00:45:00,460 --> 00:45:03,740 +اول حاجة رقم واحد بيشعر انه home sickness النفسية + +612 +00:45:03,740 --> 00:45:07,400 +تبتدش بتكون في depression لما يكون في depression + +613 +00:45:08,180 --> 00:45:11,360 +بيكون فيه low immunity خدوا بالك low immunity هذا + +614 +00:45:11,360 --> 00:45:16,400 +واحد رقم اتنين جايين من مناطق مختلفة و سكننا مع + +615 +00:45:16,400 --> 00:45:20,780 +بعض سكن مع بعض مجموعة العادات الغذائية تبقاتنا + +616 +00:45:20,780 --> 00:45:24,580 +مختلفة عن المكان و عن بعضنا البعض و الميكروبات + +617 +00:45:24,580 --> 00:45:29,300 +اللي في أنوفنا او على جلبنا برضه مختلفة فغالبا في + +618 +00:45:29,300 --> 00:45:33,320 +التجمعات اللي من هذا النوع زي الجنود زي ما قلنا او + +619 +00:45:33,320 --> 00:45:37,870 +الطلبة اللي ساكنين جدات في سكن جامعيبتوفر عندهم + +620 +00:45:37,870 --> 00:45:42,670 +ظروف من ال crowdness و ال low immunity بحيث يسمح + +621 +00:45:42,670 --> 00:45:46,110 +لانتشار كتير من الأوراق، مش بس .. مش بس اللي هو ال + +622 +00:45:46,110 --> 00:45:49,770 +.. ال .. ال cavalry unit او ال army بلقس، زمان كان + +623 +00:45:49,770 --> 00:45:53,830 +الحجاج أكتر ناس إصابة بمين بال .. بالmicrobial، + +624 +00:45:53,830 --> 00:45:56,970 +لذلك أي واحد، لأن من فلسطين أو من باكرا، حاجة + +625 +00:45:56,970 --> 00:45:59,670 +عالية، مدرحش بياخد ال vaccine تبع مين، تبع ان + +626 +00:45:59,670 --> 00:46:04,450 +سوريا من ال device، تمام؟يبقى لما أنا قلت في أول + +627 +00:46:04,450 --> 00:46:08,410 +محاضرة susceptible population army recruits + +628 +00:46:08,410 --> 00:46:13,370 +college students are susceptible groups طبعا + +629 +00:46:13,370 --> 00:46:20,570 +susceptible groups طيب غالبا كيف جثة حياة + +630 +00:46:20,570 --> 00:46:24,590 +البكتيريا دي؟ هي موجودة للأسف كمان هنا فاكرين + +631 +00:46:24,590 --> 00:46:29,680 +نظرية روبرت كوخ فرضية روبرت كوخلما كنا يجب ان يكون + +632 +00:46:29,680 --> 00:46:35,220 +ال microbe في الحيوان المصاب و ليس السليم كنا هاد + +633 +00:46:35,220 --> 00:46:39,580 +المبادئ للأسف X لأن هي مثلا نيسيريا مينيف داينس + +634 +00:46:39,580 --> 00:46:44,100 +can be present in up to 15% of the healthy + +635 +00:46:44,100 --> 00:46:47,780 +population يعني لو ميا احنا هنا أخدنا من أنوفهم + +636 +00:46:47,780 --> 00:46:53,260 +مسحات هنجي على الأقل في 15% منهم نيسيريا مينيف + +637 +00:46:53,260 --> 00:46:55,600 +داينس normal microbiome + +638 +00:46:58,170 --> 00:47:00,910 +طب what .. what .. what goes wrong؟ قلنا ال immune + +639 +00:47:00,910 --> 00:47:06,550 +system بيكون انخفض، بيكون انخفض فبتدى، اول حاجة + +640 +00:47:06,550 --> 00:47:09,310 +بتعملها بتعمل colonization في ال nasopharynx، + +641 +00:47:09,310 --> 00:47:14,790 +attachment and colonization، بعدين can reach the + +642 +00:47:14,790 --> 00:47:19,050 +bloodstreamإذا وصلنا للدم في condition اسمه + +643 +00:47:19,050 --> 00:47:21,930 +septicemia بتبقى نقوله بس عشان أنميز ال septicemia + +644 +00:47:21,930 --> 00:47:24,570 +تبعتها عن أي نوع من ال septicemia بسموها + +645 +00:47:24,570 --> 00:47:32,230 +meningococcemia meningococcemia وهي من أخطر أنواع + +646 +00:47:32,230 --> 00:47:37,270 +ال septicemia و كثير من المرضى بيموتوا عند البرحلة + +647 +00:47:37,270 --> 00:47:43,290 +هذه meningococcemia بصير عنده بُقع دموية بسموها + +648 +00:47:43,290 --> 00:47:44,370 +Petricial rashes + +649 +00:47:47,040 --> 00:47:50,600 +يعني بيصير فيه disseminated intravascular .. مر + +650 +00:47:50,600 --> 00:47:53,340 +معاك بالمصطلح disseminated intravascular + +651 +00:47:53,340 --> 00:47:59,000 +coagulation تخطرات منتشرة في كل الجسم تمام؟ بتسكر + +652 +00:47:59,000 --> 00:48:02,440 +الكابليريز أو بتنفجر الكابليريز تمام؟ في عندنا + +653 +00:48:02,440 --> 00:48:06,720 +مجموعة من الأعراض اللي بتنتجها هذه البكتيريا مابتش + +654 +00:48:06,720 --> 00:48:11,160 +المريض من .. من الجوكسيميا تتطور و تروح على ال + +655 +00:48:11,160 --> 00:48:15,520 +brain و بتعملنا ال meningitis تمام؟ و تذكره دائما + +656 +00:48:16,190 --> 00:48:21,910 +إذا الـ meningitis أي خلية ماتت لن تعوض، ماعناش + +657 +00:48:21,910 --> 00:48:24,770 +regeneration اللي خلالها ال nerve cell صح ولا لأ؟ + +658 +00:48:24,770 --> 00:48:29,050 +ماعناش regeneration، هو بالتالي ال damage بيكون + +659 +00:48:29,050 --> 00:48:32,490 +يعني كبير لما المرض بيكون ميت، عشان هيك اللي بنقول + +660 +00:48:32,490 --> 00:48:35,110 +دايما عينة ال CSF يجب أن تعمل في المختبر ك + +661 +00:48:35,110 --> 00:48:39,570 +emergency، يترك العينة الأخرى، يترك أخرى، أقولكوا + +662 +00:48:40,350 --> 00:48:43,730 +في الأماكن اللي بتحترم نفسها قبل ما يسحبوا العينة + +663 +00:48:43,730 --> 00:48:46,410 +بتتصلوا في المقتبرة تقولوا هم جاهزوا حالكوا فيه أن + +664 +00:48:46,410 --> 00:48:51,990 +عينات CSF حتى يعني إذا كان من الجامعة على وصولها + +665 +00:48:51,990 --> 00:48:56,710 +كان في ده ناشي نخلصه و نتفرج انتظارا ل .. لذلك في + +666 +00:48:56,710 --> 00:49:02,430 +ال CSF بدنا نعرف كمان إنه كل ما قصرنا فترة التشخيص + +667 +00:49:02,430 --> 00:49:08,660 +كل ما كان عندنا ال .. ال damage أقللأنه تخيلوا ان + +668 +00:49:08,660 --> 00:49:13,420 +انا اعمل مزرعة بتنتظر 72 ساعة عشان اعطي مريض نتائج + +669 +00:49:13,420 --> 00:49:17,760 +و sensitivity لأ هم بياخدوا العينة و بيبدأوا علاج + +670 +00:49:17,760 --> 00:49:23,920 +based on best guess افضل تخمين .. افضل تخمين .. + +671 +00:49:23,920 --> 00:49:26,900 +تخمين الطبيب غير تخمين الراجل من الشارع التخمين + +672 +00:49:26,900 --> 00:49:30,900 +بيكون عادى عن مين؟ عن .. عن clinical picture انه + +673 +00:49:30,900 --> 00:49:33,120 +.. و غير ما بيعطوا broad spectrum في هذه الحياة + +674 +00:49:33,120 --> 00:49:39,730 +لحين ظهور من نتائج المزرعةفي عندنا دخول عبر ال + +675 +00:49:39,730 --> 00:49:41,770 +respiratory track و بعدين على ال blood stream و + +676 +00:49:41,770 --> 00:49:44,670 +بعدين على ال central nervous system في انا حاضر في + +677 +00:49:44,670 --> 00:49:49,530 +يوتيوب اتنين يوتيوب ياريت تتفرج عليهم في المنزل من + +678 +00:49:49,530 --> 00:49:52,610 +المحاضرة المصورة او من ال presentation موجودة عندك + +679 +00:49:52,610 --> 00:49:54,130 +و ده موجودة عندك تطلع عليه + +680 +00:49:57,420 --> 00:50:00,420 +ال short period تبعتها ال incubation من واحد لاربع + +681 +00:50:00,420 --> 00:50:03,360 +أيام second most common meningitis طبعا ال + +682 +00:50:03,360 --> 00:50:06,240 +pneumococcus most common بس خدوا بالكوا كلمة most + +683 +00:50:06,240 --> 00:50:10,360 +common و second most common هذه متغيرة و country + +684 +00:50:10,360 --> 00:50:13,900 +specific يعني بمعنى اخر ممكن تكون number one في + +685 +00:50:13,900 --> 00:50:18,520 +بلد number two في بلد تانية ليش انا اقولكوا الكلام + +686 +00:50:18,520 --> 00:50:22,930 +هذا لإن مثلافي الـ 2011 دخلنا ال .. ال .. ال .. ال + +687 +00:50:22,930 --> 00:50:25,310 +.. ال vaccine تبع الـ streptococcus pneumonia أكيد + +688 +00:50:25,310 --> 00:50:27,570 +ال Epidemiology تبع ال streptococcus pneumonia + +689 +00:50:27,570 --> 00:50:31,770 +هيختلف، حد بطل ال number one، إذا بدنا نعرف إن هذا + +690 +00:50:31,770 --> 00:50:36,270 +مش قوانين مائة بالمائة فاتل + +691 +00:50:36,270 --> 00:50:38,870 +if untreated، فاتل إن القاتل لا يقدر أن يتعالج، + +692 +00:50:38,870 --> 00:50:41,910 +respond well to antibiotic therapy زي اللي هو ال + +693 +00:50:41,910 --> 00:50:47,450 +penicillin طبعا معلومة و نختم فيها ال penicillin + +694 +00:50:47,450 --> 00:50:54,790 +لا يستطيع اختراقالـ block brain barrier لكن لما + +695 +00:50:54,790 --> 00:50:59,110 +يكون الـ meninges inflamed بيهارتها بأساسها + +696 +00:50:59,110 --> 00:51:04,890 +فالبنسيلين مجرد يخترق لذلك بنعالج بالبنسيلين و + +697 +00:51:04,890 --> 00:51:07,750 +بنمنع الكاريش اشعر فيه الكاريش اللي هو ال carrier + +698 +00:51:07,750 --> 00:51:11,830 +state بانتبايتك اخر بانتبايتك اخر فبنعالج + +699 +00:51:11,830 --> 00:51:15,270 +بالبنسيلين و بنمنع يعني بنضمن ان المرض هذا مايعديش + +700 +00:51:15,270 --> 00:51:18,010 +الاخرين او ماينجلش انه يسيريا من الديسن + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/EVwb_DZpLUo_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/EVwb_DZpLUo_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..2990f49d66ee74364f69e2d211ffea2ceb34226c --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/EVwb_DZpLUo_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 3486, "start": 20.64, "end": 34.86, "text": "بسم الله الرحمن الرحيم استكمال اللي مبلقناها من الـ Gram Negative Bacilli و اذكركم بالتفصيلات اللي في 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00:00:38,150 +Negative Bacilli بناء على فحصين ال Oxidaseقسمنا + +5 +00:00:38,150 --> 00:00:41,810 +البكتيريا إلى أربع أصناف الصين في الأول كان + +6 +00:00:41,810 --> 00:00:45,230 +Oxidase positive، glucose positive، fermenter + +7 +00:00:45,230 --> 00:00:48,430 +يعني، وكان هو ال Vibrio وعلى النقيد من الجهة + +8 +00:00:48,430 --> 00:00:51,210 +التانية Oxidase negative، glucose negative، + +9 +00:00:51,210 --> 00:00:55,530 +acinetobacter وفي النص Oxidase negative، glucose + +10 +00:00:55,530 --> 00:00:59,750 +positive، enterobacteriosis Oxidase positive، + +11 +00:00:59,750 --> 00:01:05,050 +glucose negative، pseudomoxالجزء الأكبر من + +12 +00:01:05,050 --> 00:01:08,130 +مفادراتنا السابق كانت مركزة كل أقل عالمين على ال + +13 +00:01:08,130 --> 00:01:12,570 +entire bacteriasis خدنا منها أذكركم أخدنا + +14 +00:01:12,570 --> 00:01:15,890 +الإشريشيا، السالمونيلا، الشيجيلا، شوية عن + +15 +00:01:15,890 --> 00:01:21,030 +الكليبسيلة و شوية عن البروتياست امام؟ اليوم هنتكلم + +16 +00:01:21,030 --> 00:01:24,930 +عن المجموعات الأخرى اللي هم ال vibrio و ال + +17 +00:01:24,930 --> 00:01:29,230 +pseudomonas و يمكن نمر على الacetobacter الصورة + +18 +00:01:29,230 --> 00:01:36,710 +اللي قدامكم يحق ببكتيريا منحنيةو في إلها flagella + +19 +00:01:36,710 --> 00:01:42,330 +و هذه الصورة غالبا نعطيها ال vibrio اللي معروف + +20 +00:01:42,330 --> 00:01:47,830 +عنها coma shape و motile bipolar flagella ال + +21 +00:01:47,830 --> 00:01:52,310 +vibrio cholera الرمز تبعها حرف C اللي هو بالفعل + +22 +00:01:52,310 --> 00:01:57,390 +كيرف بس لاي كيرف بس لاي لو طلع الصورة قدامنا + +23 +00:01:57,390 --> 00:02:03,460 +بتوضحلنا المعنى الحقيقي لكلمة choleracholera هو + +24 +00:02:03,460 --> 00:02:08,300 +مرض gastrointestinal tract infection characterized + +25 +00:02:08,300 --> 00:02:12,760 +by severe diarrhea and severe dehydration و ال + +26 +00:02:12,760 --> 00:02:16,240 +dehydration هو اللي بيقدّقنا الى الوفيات في + +27 +00:02:16,240 --> 00:02:20,380 +الأطفال هنشوف كمان شوية وين منتشر ال cholera و لحد + +28 +00:02:20,380 --> 00:02:25,600 +الآن في عالمنا الحالي وين ال cholera موجودة لكن + +29 +00:02:25,600 --> 00:02:26,460 +تذكروا دائما + +30 +00:02:32,210 --> 00:02:35,650 +والجملة الذهبية اللى قلنا عنها اللى هو ال safe أو + +31 +00:02:35,650 --> 00:02:40,850 +ال unsafe drinking water يعني مياه شروب غير آمنة + +32 +00:02:40,850 --> 00:02:45,930 +غير نظيفة توقعوا أن يكون فيه انتشار لمرض الكورة هل + +33 +00:02:45,930 --> 00:02:52,630 +فى فلسطين احنا محمين من مرض الكورة قبل يعني حوالى + +34 +00:02:52,630 --> 00:02:58,790 +تلاتاشر عام او اربعاشر عام صار هنا وباء فى قطع غزة + +35 +00:02:59,780 --> 00:03:04,160 +هذا الوباء يعني لم يطول بالزمان وحوصر الحمد لله رب + +36 +00:03:04,160 --> 00:03:07,760 +العالمين وربنا نجانا منه يعني نسبة الوفيات كانت في + +37 +00:03:07,760 --> 00:03:11,980 +اضن حالة او حالتين فقط اتوفوا وباقي الحالات شفيت + +38 +00:03:11,980 --> 00:03:15,940 +بامر الله سبحانه وتعالى ومن ثم بجهود المخلصين من + +39 +00:03:15,940 --> 00:03:19,000 +وزارة الصحة ووكالة الغواط وحتى المؤسسات التعليمية + +40 +00:03:19,000 --> 00:03:23,880 +أيامها سهمت في الحد من انتشار هذا المرض + +41 +00:03:27,460 --> 00:03:32,420 +يعني لو توفرت الظروف مرة أخرى قد يحدث مرة أخرى و + +42 +00:03:32,420 --> 00:03:35,480 +تتذكر محاضرة السبق لما اتكلمنا عن الحروب و ما هي + +43 +00:03:35,480 --> 00:03:41,520 +أصحبها من اختلاف في مصادر المياه و نوعية هذه + +44 +00:03:41,520 --> 00:03:48,140 +المياه القصة تبدأ دائما و أبدا ب drinking of dirty + +45 +00:03:48,140 --> 00:03:53,880 +أو contaminated waterو احنا طبعا الماء اللى بيصلنا + +46 +00:03:53,880 --> 00:03:57,040 +الى المنزل اللى بيصلنا مابنعرفش من مين مصدر، + +47 +00:03:57,040 --> 00:04:00,100 +عارفين من أين جاية؟ من كوكب المريخ ولا من .. مش + +48 +00:04:00,100 --> 00:04:02,940 +عارفين انتوا من أين جاية، لكن في النهاية في جهة + +49 +00:04:02,940 --> 00:04:07,490 +مسئولة عن إيصالهو هذا الجهة is responsible .. is + +50 +00:04:07,490 --> 00:04:10,570 +responsible و أنا حكيتلكوا المرموجة انه كل + +51 +00:04:10,570 --> 00:04:16,310 +البلديات تخضع لعملية فحص دورية في مختبر الصحة + +52 +00:04:16,310 --> 00:04:19,470 +العامة بمعنى ان الماء اللي بيصنع غالبا بيكون + +53 +00:04:19,470 --> 00:04:24,370 +politically checked لكن في مناطق زي الصورة اللي + +54 +00:04:24,370 --> 00:04:28,910 +بتاعة اللي مصدر مياه الشور تبعها ممكن يكون river + +55 +00:04:28,910 --> 00:04:36,160 +يعني مصدر عامو أنا بقول دايما انه الإنسان على مدار + +56 +00:04:36,160 --> 00:04:40,380 +التاريخ أينما ورد الماء ورد الإنسان وورد الحضارة و + +57 +00:04:40,380 --> 00:04:43,820 +أينما ورد الإنسان ورد الحضارة تلوث الماء معروفة + +58 +00:04:43,820 --> 00:04:47,460 +القصة لأنه بيلجها الماء الجارى فبصير يتخلص من + +59 +00:04:47,460 --> 00:04:51,040 +النفايات تبعته في المياه الجارية صحيح المياه + +60 +00:04:51,040 --> 00:04:56,640 +الجارية إلها قدر التطهير ذاتي زي البحر مثلا لكن + +61 +00:04:56,640 --> 00:05:02,770 +كمان مرة قدر التطهير محدودةبمعنى إنه لا تستطيع .. + +62 +00:05:02,770 --> 00:05:06,910 +إذا تجوزنا هذه القدرة فهيصبح هناك أثر اسمه + +63 +00:05:06,910 --> 00:05:10,950 +contamination و pollution و هيحيطش عنه أمراض تنتقل + +64 +00:05:10,950 --> 00:05:18,790 +إلى الإنسان صورة أخرى تعبر هذه الصورة اللي في + +65 +00:05:18,790 --> 00:05:23,140 +الأعلىواضح انه فيه مياه sewage و في نفس اللي وجدت + +66 +00:05:23,140 --> 00:05:26,960 +فيه مواسير او اللي هي القنابيب اللي فيها مياه + +67 +00:05:26,960 --> 00:05:29,840 +الشرب و الأطفال بيلهوا في هذه المنطقة فبيختلف اكيد + +68 +00:05:29,840 --> 00:05:33,140 +مياه الشرب مع مياه اللي هو ال sewage الصورة اللي + +69 +00:05:33,140 --> 00:05:38,400 +أسفل بتوضح انه لما يكون الاستخدام متعدد لنفس + +70 +00:05:38,400 --> 00:05:42,220 +المصدر تبع المياه في منطقة و هو المنطقة اللي قدامي + +71 +00:05:42,220 --> 00:05:44,900 +شوية بيكون بيستخدموا المياه الشرب وبالتالي ال + +72 +00:05:44,900 --> 00:05:49,960 +pollution بينتقل من المنطقة إلى منطقة أخرىطيب، + +73 +00:05:49,960 --> 00:05:52,820 +رأينا ما يكفي من الصور، نبدأ الأن نتكلم عن + +74 +00:05:52,820 --> 00:05:56,840 +المعلومات ال Vibrio في شكل عام، مش الكوليرا، هي + +75 +00:05:56,840 --> 00:06:00,920 +Gram Negative بس لاي، هنوانها Curved or Comma + +76 +00:06:00,920 --> 00:06:03,640 +Shaped Nonsense Performing، كل المعلومات لحد الآن + +77 +00:06:03,640 --> 00:06:07,500 +بسيطة ومعروفة، Highly Motile، لكن الموتوري تبعتها + +78 +00:06:07,500 --> 00:06:11,400 +الأن بدأت أن اختلف، كنا بالأول نتكلم عن Peritricus + +79 +00:06:11,400 --> 00:06:13,540 +Flagella في الفيلم Terabacterius، الآن نتكلم عن + +80 +00:06:13,540 --> 00:06:19,090 +Single Polar Flagellait is associated with salt + +81 +00:06:19,090 --> 00:06:21,870 +water أول بكتيرة من دروسها اللي لها علاقة بالمياه + +82 +00:06:21,870 --> 00:06:26,530 +المالحة يعني البحر ممكن ينجل من هنا يعني مش هتموت + +83 +00:06:26,530 --> 00:06:29,230 +في البحر مش هتموت في البحر اللي أنا قاعد بقولكوا + +84 +00:06:29,230 --> 00:06:33,470 +أنه عنده قدرة تطهير لأنه عندها tolerance كويس + +85 +00:06:33,470 --> 00:06:38,410 +للملح عندها tolerance يعني تحمل لالملوحة it is + +86 +00:06:38,410 --> 00:06:44,370 +oxidized austin يعني برضه كمان اختلاف عن ال انترو + +87 +00:06:44,370 --> 00:06:45,810 +بكتيرات اللي كنا ندرسها قبل قليل + +88 +00:06:50,030 --> 00:06:55,010 +Tolerate alkaline هذه أول صفة أو أميز صفة في ال + +89 +00:06:55,010 --> 00:06:58,550 +vibrio على الأطلاق انه it can tolerate alkaline + +90 +00:06:58,550 --> 00:07:02,850 +conditions وبالتالي وبالتالي بدنا نستغل هذه الصفة + +91 +00:07:02,850 --> 00:07:06,210 +to selectively isolate this organism انتوا عارفين + +92 +00:07:06,210 --> 00:07:10,950 +تتكلم عن sewage او stool او water وكل المصادر اللي + +93 +00:07:10,950 --> 00:07:13,630 +ذكرتهم انا بيحتووا على مش single microbe بيحتووا + +94 +00:07:13,630 --> 00:07:17,240 +على multiple microbesto selectively isolate vibrio + +95 +00:07:17,240 --> 00:07:21,120 +you should provide alkaline conditions لأن هي + +96 +00:07:21,120 --> 00:07:25,240 +الوحيدة اللي could thrive او survive و الباقية + +97 +00:07:25,240 --> 00:07:28,380 +هيموت فبالتالي بنعتبرها enrichment او selective + +98 +00:07:28,380 --> 00:07:34,540 +media لل vibrio كمان هاد المثال طبعا في تفسير كمان + +99 +00:07:34,540 --> 00:07:40,160 +شوية هيمروا معانا معلومة بتقول ان الجرعة المؤدية + +100 +00:07:40,160 --> 00:07:45,830 +فاكرهش جلة من عشرين لإمتانهذه بدها من مائة مليون + +101 +00:07:45,830 --> 00:07:51,910 +لألف مليون، مائة مليون خلية عشان نفسر عنها كلها، + +102 +00:07:51,910 --> 00:07:58,850 +طبعا؟ معناه الحكي .. بنفسر هذا الكلام لأن ال + +103 +00:07:58,850 --> 00:08:02,630 +bacteria very sensitive to the stomach acid فمعظم + +104 +00:08:02,630 --> 00:08:07,270 +الجرع اللي بناخدها بتموتعشان تعطي فرصة لل survival + +105 +00:08:07,270 --> 00:08:11,530 +كل مزارعة البكتيريا اللى داخل كل مزارعة فرصتها انه + +106 +00:08:11,530 --> 00:08:14,010 +two or three organisms to survive to the + +107 +00:08:14,010 --> 00:08:17,550 +intestinal tract فمعظم الفيبلو بتموت وين؟ فى ال + +108 +00:08:17,550 --> 00:08:21,470 +stomach من اللى هو ال acid لإن هى بتفضل الظروف من + +109 +00:08:21,470 --> 00:08:25,110 +كم مرة إلى الكره هذا واحد رقم اتنين ممكن نستفيد من + +110 +00:08:25,110 --> 00:08:30,850 +المعلومة بإن لو في زمن الكوليرا في زمن الكوليرا او + +111 +00:08:30,850 --> 00:08:33,890 +الاوبيا تبعت الكوليراإن لو بدنا نوفر مياه قامة لو + +112 +00:08:33,890 --> 00:08:36,730 +ماعناش إمكانية لقتلها بالكلورة ممكن نقتلها بال + +113 +00:08:36,730 --> 00:08:43,410 +acid، ضفنا خل على الماء أو ضفنا ليمون، قطرات من + +114 +00:08:43,410 --> 00:08:46,110 +الليمون بيخفضوا البيئات وبالتالي بنقتل اللي هو ال + +115 +00:08:46,110 --> 00:08:51,470 +vibrio كلها، البتية ده مش صعب إنه نميهايعني لما + +116 +00:08:51,470 --> 00:08:55,110 +احنا نازلها على ال selective media بناخدها و + +117 +00:08:55,110 --> 00:08:57,550 +بنزلها على نطرة أجارة عادي، على بلد أجارة عادي، + +118 +00:08:57,550 --> 00:08:59,830 +على مقومكي عادي، لكن المشكلة وين ان؟ to + +119 +00:08:59,830 --> 00:09:04,290 +selectively isolate the organism، انه نازلها من .. + +120 +00:09:04,290 --> 00:09:08,450 +من خلطة، وكنا علي هذا الغلط بدنا نستخدم اللي هو ال + +121 +00:09:08,450 --> 00:09:15,450 +alkaline pH three species من ال vibrio اللي بدنا + +122 +00:09:15,450 --> 00:09:20,490 +نتطلع عليهمطبعا احنا هنتنب القولة التانية والتالتة + +123 +00:09:20,490 --> 00:09:24,790 +هي أقصى حد من المعلومات اللي هنعطيها الفصل هذا ال + +124 +00:09:24,790 --> 00:09:28,030 +vibricular which causes gastroenteritis اللي بسميه + +125 +00:09:28,030 --> 00:09:32,410 +احنا كلنا يعني من خطب مميزة عن بقى ال + +126 +00:09:32,410 --> 00:09:34,610 +gastroenteritis لأنها بالفعل مميزة + +127 +00:09:38,160 --> 00:09:45,080 +viproparahemolyticus و هذه بتنتقل mainly من تناول + +128 +00:09:45,080 --> 00:09:50,480 +الأسماك الغير مطهية أو غير المطهية، قاعدة بيأكل + +129 +00:09:50,480 --> 00:09:55,420 +سمك نهي؟ sushi، ففي ناس بيقول sushi قاعدين، + +130 +00:09:55,420 --> 00:10:00,620 +ثقافتهم يوانيةاليابانيين وثقافتهم بالسوشي انتقلت + +131 +00:10:00,620 --> 00:10:03,980 +إلى كل العالم وصبحت عدوى الموجودة في كل الدنيا + +132 +00:10:03,980 --> 00:10:06,880 +المطاعم اليابانية اللي بتقدم اللي هو السوشي او + +133 +00:10:06,880 --> 00:10:11,580 +الطعام او الأسماك اللي غير مافيه طبعا تتذكروا ان + +134 +00:10:11,580 --> 00:10:16,480 +انا اتكلمت ان ال Vibrio is associated with marine + +135 +00:10:16,480 --> 00:10:20,380 +او salt water يعني بحرية وبالتالي ممكن تنتقل عن + +136 +00:10:20,380 --> 00:10:23,940 +طريق اللي هو الأسماك وبتعمل gastroenteritis, wound + +137 +00:10:23,940 --> 00:10:29,550 +infection and bacteriaالاخيرة Vibrio Bolnificus + +138 +00:10:29,550 --> 00:10:32,050 +بصحيح أنه هذي من ال .. يعني من الميكروباط اللي + +139 +00:10:32,050 --> 00:10:35,690 +بتعتبر يعني إيه جديد عرفنا أنها بتعمل infection، + +140 +00:10:35,690 --> 00:10:37,730 +كلمة جديدة يعني عشرين سنة، خمس و عشرين سنة يعني، + +141 +00:10:37,730 --> 00:10:39,750 +تمام؟ هي جديد معناها، لأن معظم ال micro .. + +142 +00:10:39,750 --> 00:10:44,170 +البكتيريا عرفناها في خمسينيات و قبل خمسينيات القرن + +143 +00:10:44,170 --> 00:10:48,690 +الماضي، تمام؟ بعد ذلك بتعتبروا أنها جديدVibrio + +144 +00:10:48,690 --> 00:10:52,030 +volnepicus بتامل severe wound infection وبكتيريميا + +145 +00:10:52,030 --> 00:10:55,130 +خاصة عند الناس اللي بيشتغلوا في الأسماك، إيش اللي + +146 +00:10:55,130 --> 00:10:58,350 +بيشتغلوا في الأسماك؟ إما بيصيدوا أسماك أو بيرغفوا + +147 +00:10:58,350 --> 00:11:02,070 +الأسماك، في طبعا في المطابق، في المطاعم، في + +148 +00:11:02,070 --> 00:11:04,810 +الأماكن الأسواق، من ببيع السمك، بينطفوا السمك، + +149 +00:11:04,810 --> 00:11:08,430 +فهدول الناس عرضة إنهم يصيبوا ال Vibrio volnepicus، + +150 +00:11:08,430 --> 00:11:11,530 +الأسماء مرة أخرى Vibrio choleraVibrio + +151 +00:11:11,530 --> 00:11:16,650 +Parahemolyticus وVibrio Bolivicus الأولى أساسا ال + +152 +00:11:16,650 --> 00:11:19,650 +.. أساسا ال .. ال .. ال .. ال Vibrio وهي الأشهر + +153 +00:11:19,650 --> 00:11:23,490 +على الإطلاق وهي الأخطر على الإطلاق وتأثيرها الصحي + +154 +00:11:23,490 --> 00:11:28,710 +على المجتمعات و الأخطر على مدار التاريخ في عندنا + +155 +00:11:28,710 --> 00:11:31,190 +مرحلة اسمه تعون، كلكم بتعرفوا عنه، الدراسة تعونه + +156 +00:11:31,190 --> 00:11:36,250 +في التاريخ لما فشل حملة نابليون وفي تعون مشهور + +157 +00:11:36,250 --> 00:11:41,350 +عندنا في فلسطين تعون اموازاللي استجت فيه أبو عبادة + +158 +00:11:41,350 --> 00:11:45,990 +عمر بن الجراح طعون أو مؤسس شهير إن الطعون مرض خطير + +159 +00:11:45,990 --> 00:11:50,790 +ومعروف وكانوا في .. في مناطق الزمنية اللي فيها جهة + +160 +00:11:50,790 --> 00:11:55,530 +الكبير لما يكون مرض قاتل يسموه طعون حتى لو كانش + +161 +00:11:55,530 --> 00:11:59,170 +طعون وفي أزمية من الأزمينة كمان كانوا يسموا + +162 +00:11:59,170 --> 00:12:03,670 +الأمراض القاتلة يسموها cholera لأن ال cholera كانت + +163 +00:12:03,670 --> 00:12:07,400 +تقتل أيضا نسبة كبيرة من الناسفكانوا يقولوا طعون أو + +164 +00:12:07,400 --> 00:12:10,740 +يقولوا كوليرة للدلالة على إن المرض يقتل أو نسبة + +165 +00:12:10,740 --> 00:12:13,920 +القتل فيه عالية جدا إذا مش المقصود في كل كوليرة + +166 +00:12:13,920 --> 00:12:17,640 +كانت تاريخيا إنها كوليرة وإنما ربما تكون مرض آخر + +167 +00:12:17,640 --> 00:12:21,740 +لكن نسبة القتل فيه عالية فأطلق عليه لقب كوليرة هذا + +168 +00:12:21,740 --> 00:12:24,900 +كلام قبل ما يتسجل العلم بال micro أو علم ال micro + +169 +00:12:24,900 --> 00:12:31,740 +virus طيب ال antigenic structure لل Vibrio cholera + +170 +00:12:32,790 --> 00:12:38,070 +تحتوي على two antigens مهمين جدا اللي هم ال O لإن + +171 +00:12:38,070 --> 00:12:41,790 +هي gram نيجاتيب بس لله، فيها ليبوكوليسكارات و فيها + +172 +00:12:41,790 --> 00:12:46,830 +H لإن فيها polar flare، تمام؟ و ان فيها حوالي 150 + +173 +00:12:46,830 --> 00:12:53,140 +serotype، بناء على مين؟ بناء على ال O-antigenلكن + +174 +00:12:53,140 --> 00:12:58,180 +من هذه ال 150 فقط two serotypes اللي هم ال O1 و ال + +175 +00:12:58,180 --> 00:13:04,700 +O139 هم اللي بيعملوا infection بالكلرة للإنسان و + +176 +00:13:04,700 --> 00:13:08,040 +للأسف .. للأسف احنا ظالمين الفيبروكلورة طلعنا + +177 +00:13:08,040 --> 00:13:13,000 +فكرنا الفيبروكلورة هي من راسها بتسبب الكلرة يعني + +178 +00:13:13,000 --> 00:13:16,920 +هي حابة تعمل معاها الكلرة لكن واقع أول لجينا انه + +179 +00:13:16,920 --> 00:13:22,280 +ال toxin اللي بيأهلها تعمل الكلرة هو phage encoder + +180 +00:13:22,910 --> 00:13:28,690 +إيش يعني؟ يعني هي lysogenized lysogenized cells + +181 +00:13:28,690 --> 00:13:34,310 +يبقى عشانك تفسر ان لماذا ال 150 serotypes كلهم مش + +182 +00:13:34,310 --> 00:13:38,250 +مهمين، مين المهم فقط منهم اللي هو اللي فيها ال pro + +183 +00:13:38,250 --> 00:13:43,150 +-fagin ال O1 و ال O139 هم دول التانية اللي موجودة + +184 +00:13:43,150 --> 00:13:46,150 +فيهم ال bacteriophage طبعا هنمرع على كده شوية في + +185 +00:13:46,150 --> 00:13:51,050 +أسماء حلوة هنسمعها الآن Ogawa + +186 +00:13:51,050 --> 00:13:57,330 +Inabaهيكوجيما انابا و أجاوة و هيكوجيما three + +187 +00:13:57,330 --> 00:14:05,250 +serotypes لمن؟ لل Vibrio cholera لل O1 كلمة + +188 +00:14:05,250 --> 00:14:10,930 +Serotype بقى فهمها أيش معناها أنه سلالة تختلف عن + +189 +00:14:10,930 --> 00:14:15,310 +سلالة أخرى عن طريق ال أنفجينة تبعتها صح ولا لأ؟ + +190 +00:14:15,310 --> 00:14:19,170 +الآن في وراها على طول في اسمة two biovar أو bio + +191 +00:14:19,170 --> 00:14:24,950 +type و biovar أو كلمة varVAR معناها type VAR جابني + +192 +00:14:24,950 --> 00:14:29,850 +ال variety variety معناها نوع فنوع او و ال type + +193 +00:14:29,850 --> 00:14:33,830 +نفس الشي فمرة تقوله serotype او serovar , biotype + +194 +00:14:33,830 --> 00:14:39,550 +او biovar اذا نفس المعنى احنا درسنا serotype و + +195 +00:14:39,550 --> 00:14:43,350 +درسنا phagotype صح اتعرفنا على البكتيرا من خلال + +196 +00:14:43,350 --> 00:14:47,570 +البكتيروفاج فكنا احنا phagotype الان بنتكلم عن + +197 +00:14:47,570 --> 00:14:54,040 +حاجة اسمها biotype biotype ما معناه biotypeSpecies + +198 +00:14:54,040 --> 00:14:57,440 +واحدة على سبيل المثال ال Vibrocula تركزوا معاها + +199 +00:14:57,440 --> 00:15:03,820 +عشان المفهوم واضح ماندوش كتابة Species واحدة لجينا + +200 +00:15:03,820 --> 00:15:07,420 +الفرق الأساسي مش في الأنتجينات وإنما في صفة + +201 +00:15:07,420 --> 00:15:09,580 +بيوكيمية كونكوا دراستوا ال Vocos Proscara في + +202 +00:15:09,580 --> 00:15:14,380 +المعمل ال VB تخيلوا إنه في تنتين من ال Vibrocula + +203 +00:15:14,380 --> 00:15:17,900 +اللي هي أوة اللي ممكن تكون إيناب أو ممكن تكون + +204 +00:15:17,900 --> 00:15:22,180 +أجابة أو ممكن تكون هيكوجيمةواحدة منهم positive + +205 +00:15:22,180 --> 00:15:25,720 +للوغس بلوس كوار وواحدة منهم negative للوغس بلوس + +206 +00:15:25,720 --> 00:15:30,240 +كوار فالمتجرش يقول عنهم هدولة Serotype وولا يقول + +207 +00:15:30,240 --> 00:15:32,320 +عنهم Fasciotype ايش يقول عنهم اختلفوا في + +208 +00:15:32,320 --> 00:15:34,900 +biochemical characteristics او physical + +209 +00:15:34,900 --> 00:15:38,260 +characteristics نسميهم biotypes .. biotypes فهي + +210 +00:15:38,260 --> 00:15:41,000 +معناه ال biotype في واحد اسمه classical او classic + +211 +00:15:41,000 --> 00:15:45,240 +والتانية ال tour .. ال tour واحدة classic واحدة ال + +212 +00:15:45,240 --> 00:15:48,140 +tour طبعا ال classic المفهوم ان هي ال original هي + +213 +00:15:48,140 --> 00:15:49,840 +القديمة المعروفة سابقة + +214 +00:15:52,390 --> 00:16:00,150 +هذا ال diagram بيوضح + +215 +00:16:00,150 --> 00:16:03,750 +أنه التقسيمة تبع ال viprocolera based on o-antigen + +216 +00:16:03,750 --> 00:16:08,010 +يكون فيهين two oxygen extracts فقط وهما 139 وهذا + +217 +00:16:08,010 --> 00:16:11,490 +يعني كمان أهميته مش كتير عالية، الأهمية لمين؟ لل + +218 +00:16:11,490 --> 00:16:16,280 +o, oneالـ O1 فيه منه two biotypes اللي هم الالتوار + +219 +00:16:16,280 --> 00:16:18,860 +و ال classical اللي حكينا عنهم و الفرق الأساسي + +220 +00:16:18,860 --> 00:16:21,640 +بينهم هو ال volume و الscore و فيه فروقات أخرى + +221 +00:16:21,640 --> 00:16:26,880 +هدوسها لاحقا كل واحد من التن هدولة قد يشتمل على + +222 +00:16:26,880 --> 00:16:32,020 +انابة أو أجابة أو هيكوجيمة طبعا أنا بأذكر بس قصة + +223 +00:16:32,020 --> 00:16:37,030 +ال هيكوجيمةكان فينا سؤالتين معروفين لما الإنابة و + +224 +00:16:37,030 --> 00:16:40,050 +الإنابة كيف كنا نتعرف عليها؟ كان في antiserum اسمه + +225 +00:16:40,050 --> 00:16:44,370 +anti-inaba نحطه علي البكتيريا إذا اتفاعل معها فهي + +226 +00:16:44,370 --> 00:16:48,890 +انابة و فينا antiserum اسمه أجاوة إذا حطيناه علي + +227 +00:16:48,890 --> 00:16:52,310 +البكتيريا اتفاعل معها لكن ال anti-inaba مابتفعلش + +228 +00:16:52,310 --> 00:16:55,190 +مع الأجاوة و الأجاوة مابتفعل مع مين؟ مع الإنابة + +229 +00:16:55,940 --> 00:16:59,260 +يعني كل واحد بالفعل سلالة بنفصل، يوم عزلنا، مش + +230 +00:16:59,260 --> 00:17:03,600 +احنا العلماء اللي بيشتغلوا عزلوا بكتيريا، حطوا + +231 +00:17:03,600 --> 00:17:07,340 +عليها أنتيقنابا بوستر، حطوا عليها أنتيقنابا بوستر، + +232 +00:17:07,340 --> 00:17:10,780 +فصارت هي بوستر لمين؟ للإنابة والأوجاوة، هذي معناه + +233 +00:17:10,780 --> 00:17:14,100 +حاجة إنه بتتكلم عن سلالة جديدة، طبعا تأكدوا، قعدوا + +234 +00:17:14,100 --> 00:17:16,280 +الفحص مره و مره و تكرهه، انفعوا طلعت سلالة جديدة و + +235 +00:17:16,280 --> 00:17:20,680 +سموها Hikoshi، تمام؟إذا القنابة وجاوة هيكوجيما + +236 +00:17:20,680 --> 00:17:24,160 +هدول عبارة عن zero types قدرلهم في ال classical أو + +237 +00:17:24,160 --> 00:17:27,620 +قدرلهم في الالتور و الالتور و الclassical هم bio + +238 +00:17:27,620 --> 00:17:34,080 +types لمين؟ لل O1 من zero type اتنين فقط من + +239 +00:17:34,080 --> 00:17:37,580 +الvibrio ال O1 و ال O139 + +240 +00:17:39,950 --> 00:17:43,590 +الفكل oral transmission هي ال route المشهودة + +241 +00:17:43,590 --> 00:17:48,070 +المعروفة في انتقال البكتيريا من ال faces اللي بتصل + +242 +00:17:48,070 --> 00:17:51,130 +ال water سواء كان fresh و ال fresh مياه عذبة يعني + +243 +00:17:51,130 --> 00:17:54,810 +المياه الحلوة تمام؟ و ال salt water اللي هي ال + +244 +00:17:54,810 --> 00:17:59,010 +marine water اللي زي مياه البحر لوّثت الغذاء لوّثت + +245 +00:17:59,010 --> 00:18:04,850 +المادة هنا تمام؟ هي طريقة الانتقال في مصطلحين + +246 +00:18:04,850 --> 00:18:08,460 +قدامنا هنا ال epidemic و ال endemicممكن ال endemic + +247 +00:18:08,460 --> 00:18:11,100 +أول مرة يقول معانا ولا ماروا معانا قبل هيك؟ مامر + +248 +00:18:11,100 --> 00:18:15,100 +معانا endemic طيب نميز بين المصطلحين و نعرف إيش + +249 +00:18:15,100 --> 00:18:17,960 +أساسا Epidemic معناها كتير من الناس ترجمة باللغة + +250 +00:18:17,960 --> 00:18:20,100 +العربية Epidemic معاناها واباة ترجمة باللغة + +251 +00:18:20,100 --> 00:18:23,820 +العربية معاناها واباة لكن مفهوم الوبا عند كتير من + +252 +00:18:23,820 --> 00:18:29,540 +الناس مفهوم خاطر بنصحح اليوم once and for all مرة + +253 +00:18:29,540 --> 00:18:35,390 +واحدة للأبد ما معناه واباةالوباء هو عبارة عن + +254 +00:18:35,390 --> 00:18:41,030 +increase in number of cases for certain disease عن + +255 +00:18:41,030 --> 00:18:45,810 +مثلاته في السنوات السابقة كيف يعني؟ تركزوا معايا + +256 +00:18:45,810 --> 00:18:49,790 +عشان تفهموها و الله أحسن من الكتابة احنا في شهر + +257 +00:18:49,790 --> 00:18:54,770 +إيش؟ احنا ديسمبر 2014 بنفرض ان احنا في أي أسبوع؟ + +258 +00:18:54,770 --> 00:18:57,970 +الأسبوع التاني؟ ولا لسه الأسبوع الأول؟ كده ايش + +259 +00:18:57,970 --> 00:19:03,330 +تاريخ اليوم؟ يعني بدأنا في الأسبوع التانيلو جينا + +260 +00:19:03,330 --> 00:19:07,090 +عملنا إحصائيات عن مرض ال influenza في فلسطين في + +261 +00:19:07,090 --> 00:19:11,890 +الأسبوع الأول من شهر ديسمبر عام 2014 لجينا 5000 + +262 +00:19:11,890 --> 00:19:16,510 +حالة جينا قررنا مع السنة الماضية اللي هي 2013 و + +263 +00:19:16,510 --> 00:19:23,870 +2012 لجينا أن طول الشهر تبع ديسمبر في 2013 لما + +264 +00:19:23,870 --> 00:19:27,270 +دلوقت الحالات أتت عالية بقعدة 500 يبقى في الأسبوع + +265 +00:19:27,270 --> 00:19:32,610 +الأولشهر اتناش في الفين واربعتاش أضعاف مضاعفة عن + +266 +00:19:32,610 --> 00:19:36,790 +مين؟ عن كل الشهر تبقى فهذا بنسميه هنا epidemic + +267 +00:19:36,790 --> 00:19:42,230 +طبعا؟ أما المفهوم اللي كان في أنكو سابقا عن الوباء + +268 +00:19:42,230 --> 00:19:46,450 +اسمه outbreak outbreak هو انتشار مفاجئ .. هو + +269 +00:19:46,450 --> 00:19:50,410 +انتشار مفاجئ لمرض معايف يسموه outbreak طبعا؟ + +270 +00:19:50,410 --> 00:19:54,630 +outbreak إذا الوباء وصل الصورة مش معناها نيجي تقلل + +271 +00:19:54,630 --> 00:19:59,880 +ال endemicendemic بالعربي معناها مستوطن، مش مستوطن + +272 +00:19:59,880 --> 00:20:04,760 +إسرائيل، لأ مستوطن .. المرض مستوطن، بمعنى إنه + +273 +00:20:04,760 --> 00:20:10,420 +بيحدث بنسب مستقرة في نفس المكان، يعني احنا عندما + +274 +00:20:10,420 --> 00:20:13,260 +.. احنا جونا .. متى صار عندنا كوليرا؟ في السابعة + +275 +00:20:13,260 --> 00:20:18,760 +وتسعين .. من سنة الف تسعين وسبعة تسعين، بس جابله، + +276 +00:20:18,760 --> 00:20:22,020 +بعرفش كوليرا، ممكن يحدث عندنا كمان خمس سنوات أو + +277 +00:20:22,020 --> 00:20:26,000 +سنتين، كمان سنة، بس مش كل يوم بيحدث عندناما بنعزلش + +278 +00:20:26,000 --> 00:20:29,620 +كورة بشكل روتيني في غزة، طبعا، لكن اللي روحنا على + +279 +00:20:29,620 --> 00:20:33,120 +الهند او باكستان او الفلبين او اندونسيا او او اي + +280 +00:20:33,120 --> 00:20:37,380 +مراتق زي بوليفيا، هنلاجئ انهم بيعزلوا بشكل روتيني + +281 +00:20:37,380 --> 00:20:42,180 +في غزة حالات بنسميها sporadic cases، ما نضحك ان + +282 +00:20:42,180 --> 00:20:48,190 +المرض مستوطن في تلك المنطقةقد يحدث وضع Epidemic في + +283 +00:20:48,190 --> 00:20:50,910 +نفس المنطقة اللي .. اللي ما يكونوا فيها إيه؟ + +284 +00:20:50,910 --> 00:20:55,270 +Endemic بمعنى إن السنة عدد الحالات تبع الكورونا + +285 +00:20:55,270 --> 00:20:59,430 +زاد على السنوات السابقة لكن في الآخر المرض إيه؟ + +286 +00:20:59,430 --> 00:21:02,730 +Endemic .. المرض Endemic وصلت إيش الفرق بين + +287 +00:21:02,730 --> 00:21:08,390 +Epidemic و Endemic؟ هذه الخارطة العالمية اللي + +288 +00:21:08,390 --> 00:21:13,510 +موجودة أمامكم بتوضحلنا إنه التركيز وين في أفريقيا + +289 +00:21:14,160 --> 00:21:20,660 +أفريقيا لإنه poor sanitary conditions ال source of + +290 +00:21:20,660 --> 00:21:24,640 +drinking water is scarce ندرة في مصادر مياه الشرب + +291 +00:21:24,640 --> 00:21:29,100 +تمام؟ وبالتالي بالفعل احتمالية الانتشار طلع العراق + +292 +00:21:29,100 --> 00:21:34,800 +البلد اللي كانت في يوم من الأيام من أقوى البلدان + +293 +00:21:34,800 --> 00:21:39,420 +العربية و أكترها تطورا لإنه صار فيها حروب و صار + +294 +00:21:39,420 --> 00:21:43,070 +فيها destructionمستوى المعيشة انخفض، مستوى الخدمات + +295 +00:21:43,070 --> 00:21:47,290 +انخفض، مستوى انتشار الكوليرة في العراف موجود الآن، + +296 +00:21:47,290 --> 00:21:50,250 +فاهم؟ و هاد الخارطة طلع .. اذا تطلع على الجزر + +297 +00:21:50,250 --> 00:21:55,170 +الصوفي اللي هنا، بتويل 2007 و 2009 و كل ما كبرت + +298 +00:21:55,170 --> 00:21:58,430 +الدائرة، كل ما زاد عني معناه لحكي عدد الحلال، يعني + +299 +00:21:58,430 --> 00:22:02,230 +حجم الدائرة هو تعبير عن عدد الحلال اللي هو الإصابة + +300 +00:22:02,230 --> 00:22:06,190 +طبعا تلاحظوا الهند .. الهند أو شبه القارة الهندية، + +301 +00:22:06,190 --> 00:22:09,250 +شوفوا أفغانستان أيضا، هنا في هنا .. في أمريكا + +302 +00:22:09,250 --> 00:22:13,820 +اللاتينية في هنابوليفيا، لكن تطلع أوروبا، تطلعوا + +303 +00:22:13,820 --> 00:22:18,880 +أمريكا، شمالية، مافيش، تمام؟ أسيا هي المصدر + +304 +00:22:18,880 --> 00:22:21,820 +الأساسي كانت للكويرة أسيا و أفريقيا، بس الآن + +305 +00:22:21,820 --> 00:22:24,720 +لاحظوا التركيز بالفعل وان موجود في أفريقيا، في + +306 +00:22:24,720 --> 00:22:30,440 +أفريقيا مافيش حاجة protected من الكويرة، اليوم + +307 +00:22:30,440 --> 00:22:34,080 +العالم كله يعتبر .. لما يوصفوه قرية صغيرة هو قرية + +308 +00:22:34,080 --> 00:22:38,130 +صغيرة، يعني مريضحامض للميكروب بيروح لأمريكا لو + +309 +00:22:38,130 --> 00:22:40,510 +توفرت الظروف المناسبة ممكن يصير عندهم برضه + +310 +00:22:40,510 --> 00:22:44,190 +epidemic في أمريكا يعني فالأمر مش مقتصر لكن هي + +311 +00:22:44,190 --> 00:22:47,170 +موضوع توفر و الظروف الملائمة اللي انتشرت على المرض + +312 +00:22:47,170 --> 00:22:51,650 +الفاعتogenesis جولنا لازم الإنسان يصير يعمل + +313 +00:22:51,650 --> 00:22:55,490 +ingestion ل 10 اقص 8 ل 10 اقص 10 microorganism + +314 +00:22:55,490 --> 00:22:58,710 +يعني من 100 مليون ل أكتر من حتى 1000 مليون 10 اقص + +315 +00:22:58,710 --> 00:23:03,290 +9 ل 1000 مليونNon-invasive infection of the small + +316 +00:23:03,290 --> 00:23:06,590 +intestine فقط ما بتروحها الدم يعني لم نعزلها من + +317 +00:23:06,590 --> 00:23:09,570 +مكان آخر غير ال intestinal tract يعني المكان اللي + +318 +00:23:09,570 --> 00:23:15,530 +بنعزل منه ال Vibrosamine زي الشجلة شجلة لم نعزلها + +319 +00:23:15,530 --> 00:23:19,530 +من أماكن أخرى غير ال intestinal tract Organism + +320 +00:23:19,530 --> 00:23:24,820 +secretes enterotoxinاللي هو ال Exotoxin المعروف + +321 +00:23:24,820 --> 00:23:29,380 +بال Vibrio ودرستوا انتوا لما درسنا البعاثة جنسيس و + +322 +00:23:29,380 --> 00:23:32,060 +ال virulence factors و لما درسنا ال Escherichia + +323 +00:23:32,060 --> 00:23:35,940 +coli أحد الأنواع سمينا enterotoxinic Escherichia + +324 +00:23:35,940 --> 00:23:41,360 +coli وقلنا it produces cholera-like toxin اللي هو + +325 +00:23:41,360 --> 00:23:48,160 +ال L-T-Toxin ال L-T تذكر ال L-T-Toxin it acts + +326 +00:23:49,330 --> 00:23:52,830 +in a similar way to that of Vibrio cholera هذا + +327 +00:23:52,830 --> 00:23:55,650 +الكلام اللي حكيناه يعني بما احنا نصلنا لأصل الكلام + +328 +00:23:55,650 --> 00:23:59,030 +عن ال Vibrio cholera هنتطلع على التكسين كيف بيشتغل + +329 +00:23:59,030 --> 00:24:02,990 +الآن لكن ال result للتكسين اللي هو ال water ايه ده + +330 +00:24:02,990 --> 00:24:07,850 +يعني بعض ال virulence factors اللي اختارناها طبعا + +331 +00:24:07,850 --> 00:24:11,170 +مش هي كل مش كلها لكن قائمة مقتصرة منهااللي هي + +332 +00:24:11,170 --> 00:24:13,950 +الكوليراتوكسين اللي حكينا عنه والبيلاي وفي بعض + +333 +00:24:13,950 --> 00:24:16,890 +الادهيسينز الأخرى فاكرين المحاضرة السابقة اتحدثت + +334 +00:24:16,890 --> 00:24:23,710 +أنا على فيمبريال ومصطلح فيمبريال ومصطلح فيمبريال + +335 +00:24:23,710 --> 00:24:27,310 +ومصطلح فيمبريال ومصطلح فيمبريال ومصطلح فيمبريال + +336 +00:24:27,310 --> 00:24:31,110 +ومصطلح فيمبريال ومصطلح + +337 +00:24:31,110 --> 00:24:32,890 +فيمبريال ومصطلح فيمبريال ومصطلح فيمبريال ومصطلح + +338 +00:24:32,890 --> 00:24:34,150 +فيمبريال ومصطلح فيمبريال ومصطلح فيمبريال ومصطلح + +339 +00:24:34,150 --> 00:24:37,050 +فيمبريال ومصطلح فيمبريال ومصطلح فيمبري + +340 +00:24:40,000 --> 00:24:42,640 +ماذا يعني بروتياز؟ بروتياز فاكرين انتوا قلتوا + +341 +00:24:42,640 --> 00:24:46,380 +هنسميه IGA بروتياز بس هذا .. هذا مختلف هذا مختلف + +342 +00:24:46,380 --> 00:24:50,940 +مش IGA بروتياز البروتياز + +343 +00:24:50,940 --> 00:24:54,200 +بشكل عام مصطرح لإنزيمات قدعت على تكسير البروتينات + +344 +00:24:54,200 --> 00:24:58,920 +لما أقول أنهيم أجلوتناشن بروتياز بقولنا الوظيفة .. + +345 +00:24:58,920 --> 00:25:01,980 +الوظيفة تبعته لو كلمة أجلوتناشن يعني بقى الحاجة + +346 +00:25:01,980 --> 00:25:07,870 +إنه بيجمع ال ..لكن بيساعد نفسه انه to release the + +347 +00:25:07,870 --> 00:25:11,130 +bacteria from membranes from mucosal cells + +348 +00:25:11,130 --> 00:25:16,670 +الميكوزا فيها ميوكاس انا فكيف بتتشربك البكتيريا + +349 +00:25:16,670 --> 00:25:22,830 +فهو بيسلكها من هذا لميوكاس لبروتين او لبروتياز ال + +350 +00:25:22,830 --> 00:25:24,530 +hemagglutination اللي هو جثة ال hemagglutination + +351 +00:25:24,530 --> 00:25:28,790 +معناته انه عبارة عن بروتين قادر لو انا حاططه على + +352 +00:25:28,790 --> 00:25:31,390 +blood cell يجمع blood cell مع بعض بعمله + +353 +00:25:31,390 --> 00:25:33,690 +agglutinationبيعملهم agglutination، بيسموه + +354 +00:25:33,690 --> 00:25:37,850 +hemagglutination، وهذه الصفة بنميز فيها ال + +355 +00:25:37,850 --> 00:25:40,970 +classical عن الالتار، عشان أقول إن الفوكس + +356 +00:25:40,970 --> 00:25:43,410 +بروسكاور، وهي ال hemagglutination كمان بنميزهم + +357 +00:25:43,410 --> 00:25:47,270 +فيها، لفلاجلوم طبعا organ of motility، وانا مئات + +358 +00:25:47,270 --> 00:25:51,550 +المرات ذكرت، قلت إن ال motility is a virulence + +359 +00:25:51,550 --> 00:25:55,210 +factor، لأنها بتبعدنا عن الخطر و بتقربنا من اللي + +360 +00:25:55,210 --> 00:25:59,020 +هو الغداءسيدلوف واروس بتعمل iron sequestration + +361 +00:25:59,020 --> 00:26:02,180 +وهذا أمر قلنا مطلوب جدا اللي هي ال iron capturing + +362 +00:26:02,180 --> 00:26:08,060 +اللي هي التقاط ال iron من و منافسة ال host عليه ال + +363 +00:26:08,060 --> 00:26:13,000 +كودراتوكسين انا لما بحطلكوا هنا link انا بحكي انه + +364 +00:26:13,000 --> 00:26:15,560 +لو ضغطوا على ال link هذا بتروح على اليوتيوب ان شاء + +365 +00:26:15,560 --> 00:26:18,300 +الله عشان تشوفوا كيف التوكسين بيشتغل يعني بيكون في + +366 +00:26:18,300 --> 00:26:24,900 +فيديو توضيح لهذا الموضوع طيبit is encoded by a + +367 +00:26:24,900 --> 00:26:30,460 +brofage يبقى التهمة سقطت عن مين؟ يعني غير محاسبة + +368 +00:26:30,460 --> 00:26:35,660 +البكتيريا على أفعارها لإنه هي اللي بيقوم بالمهمة + +369 +00:26:35,660 --> 00:26:39,680 +القادرة على هذه البكتيريا هو ال bacteriophage اللي + +370 +00:26:39,680 --> 00:26:42,800 +بيعطيها المقدر على إنتاج التوكسين خلاص براء ال + +371 +00:26:42,800 --> 00:26:47,200 +Bible كلنا اليوم ال molecular mass للتوكسين هو + +372 +00:26:47,200 --> 00:26:53,300 +عبارة عن 84 ألف دالتونوبيتكون من two sub units + +373 +00:26:53,300 --> 00:26:58,030 +فاكرينهم ال a sub unit و ال b sub unitالـ A اللي + +374 +00:26:58,030 --> 00:27:00,270 +هي ال active اللي هي ال toxic و ال B اللي هي ال + +375 +00:27:00,270 --> 00:27:01,910 +binding تعالى نطلع على ال binding، بيقولنا ال + +376 +00:27:01,910 --> 00:27:05,670 +binding ال receptor استبعته هي ال GM1-Ganglioside + +377 +00:27:05,670 --> 00:27:08,070 +اللي موجودة على ال enterocytes ال enterocytes دي + +378 +00:27:08,070 --> 00:27:11,450 +هي الخلايا اللي موجودة في ال intestinal tract فيها + +379 +00:27:11,450 --> 00:27:16,950 +GM Protein أو بيسموه GM1-Ganglioside Protein ال P + +380 +00:27:16,950 --> 00:27:21,330 +-subunit ترتبط فيه هذا معناه حكي أنه التكسين بيدخل + +381 +00:27:21,330 --> 00:27:24,550 +في هذه الخلايا اللي هي اسمها enterocytes إيش بيعمل + +382 +00:27:24,550 --> 00:27:30,450 +التكسين؟بيعمل حاسم ADP-ribosylation تاكرين كلمة + +383 +00:27:30,450 --> 00:27:36,130 +ADP-ribosylation أنا ذكرتها في .. تمام مثلا .. + +384 +00:27:36,130 --> 00:27:42,610 +مثلا أنا .. مثلا أنا بدي .. بدي واضح نقطة هذا أن + +385 +00:27:42,610 --> 00:27:47,130 +ال ADP-ribosylation ممكن تكون عملية من عملية تانية + +386 +00:27:47,130 --> 00:27:50,270 +يما switch on يما switch off يعني يا activation يا + +387 +00:27:50,270 --> 00:27:56,810 +دي activation بتطلع زي واحدة بتغطي زرالكبسة او + +388 +00:27:56,810 --> 00:28:00,150 +الضغط هي ال ADP-ribosylation كلامك صحيح ال ADP + +389 +00:28:00,150 --> 00:28:04,050 +-ribosylation بعمل activation لأحد المركبات اللي + +390 +00:28:04,050 --> 00:28:08,370 +بتنشط الأدنية الساكنية تنزعها فبينشط الأدنية + +391 +00:28:08,370 --> 00:28:12,350 +الساكنية تنزعها إذا نشط بيتحول في النشاط هذا من ال + +392 +00:28:12,350 --> 00:28:16,690 +ATP ال ATP لمين؟ لساكنية EMP وبالتالي + +393 +00:28:19,560 --> 00:28:24,020 +اللملة الأخيرة المشهورة اللي صار عنها دايما انفسر + +394 +00:28:24,020 --> 00:28:26,260 +فيها اللي هو ال accumulation of electrolytes اللي + +395 +00:28:26,260 --> 00:28:29,360 +diluted بيه اللي هو كميات كبيرة أو copious amount + +396 +00:28:29,360 --> 00:28:34,100 +of water ال + +397 +00:28:34,100 --> 00:28:38,020 +vibrocholera clinical manifestation بقولنا may + +398 +00:28:38,020 --> 00:28:41,100 +range from asymptomatic colonization to fatal + +399 +00:28:41,100 --> 00:28:44,940 +diarrhea يبقى فيه خشية من ال asymptomatic + +400 +00:28:44,940 --> 00:28:47,140 +colonization اللي سميناها carrier + +401 +00:28:50,600 --> 00:28:54,520 +يعني واحد career و لا يبدو عليه اي علامات انه مصاب + +402 +00:28:54,520 --> 00:28:58,880 +لكن هو بيعمل shedding بيفرز الكوليرا في ال stool + +403 +00:28:58,880 --> 00:29:04,660 +تبقى وبالتالي هو قد يشكل مصدر عدو لمين للاخرين ال + +404 +00:29:04,660 --> 00:29:07,840 +onset يعني النوبة بتحدث من اتنين لتلت أيام after + +405 +00:29:07,840 --> 00:29:11,020 +ingestion يعني incubation period قصيرة جدا من + +406 +00:29:11,020 --> 00:29:14,740 +اتنين لتلت أيام لحاجة للبكتيريا عند المقدرة تكاتب + +407 +00:29:14,740 --> 00:29:21,000 +شكل مهول في ال intestinal tractupropt يعني مفاجئ + +408 +00:29:21,000 --> 00:29:27,300 +onset of water diarrhea and vomiting في فيديو + +409 +00:29:27,300 --> 00:29:31,080 +حاطولكوا يعني مش عارف في مكاننا عن educational + +410 +00:29:31,080 --> 00:29:37,200 +فيديو بالكارتون ووضح لنا كيف أن طفل أبوه أصيب + +411 +00:29:37,200 --> 00:29:42,780 +بالكلرة و ب simple procedures ب simple توعية في + +412 +00:29:42,780 --> 00:29:49,860 +المجتمع تبعه قضى على الكلرة الفيديو حلو طلع عليههو + +413 +00:29:49,860 --> 00:29:52,360 +حاجة بس عين كيك هو يمكن يكون في كتير water دياريا + +414 +00:29:52,360 --> 00:29:55,360 +و vomiting في البيت بس تحذير يا عمينا عملكوا + +415 +00:29:55,360 --> 00:29:59,220 +warning rice water stool rice water stool يقولنا + +416 +00:29:59,220 --> 00:30:02,780 +هدى characteristic للسطول للمرضى اللى عندهم + +417 +00:30:02,780 --> 00:30:07,880 +cholera ايش هدا rice water stool يعني شكل وقوان ال + +418 +00:30:07,880 --> 00:30:08,800 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +419 +00:30:08,800 --> 00:30:09,700 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +420 +00:30:09,700 --> 00:30:11,840 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +421 +00:30:11,840 --> 00:30:12,320 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +422 +00:30:12,320 --> 00:30:12,520 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +423 +00:30:12,520 --> 00:30:12,800 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +424 +00:30:12,800 --> 00:30:12,860 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +425 +00:30:12,860 --> 00:30:19,680 +ال .. ال .. ال .. ال .. ال .. ال .. الsevere fluid + +426 +00:30:19,680 --> 00:30:24,720 +and electrolyte loss أو loss بيقدر ال dehydration، + +427 +00:30:24,720 --> 00:30:27,960 +metabolic acidosis، hypervolemic shock، + +428 +00:30:27,960 --> 00:30:31,840 +hypervolemic shock، لأن المي خلاص، الدم بصير thick + +429 +00:30:31,840 --> 00:30:36,300 +أصلا، تمام؟ و Renal failure، لأن ال Renal failure + +430 +00:30:36,300 --> 00:30:40,730 +ممكن يحدث من combination، من اللي هوالـ water loss + +431 +00:30:40,730 --> 00:30:43,010 +و ال electrolyte imbalance عدم اتزان في ال + +432 +00:30:43,010 --> 00:30:48,470 +electrolyte تخيل يا بنات نسبة الوفيات 60% if + +433 +00:30:48,470 --> 00:30:54,110 +untreated one percent if treated يعني خفضنا .. + +434 +00:30:54,110 --> 00:30:57,190 +خفضنا .. النسبة الوفيات بدون انتباه تفقد بالك + +435 +00:30:57,190 --> 00:31:03,810 +نتكلم عليا ساهمين على اللي هو فقط تعويض السوائل و + +436 +00:31:03,810 --> 00:31:07,520 +ال .. و نعنهم ال electrolyte وبالتاليالحاجة اللى + +437 +00:31:07,520 --> 00:31:12,320 +قلتلكوا ب simple instructions منعنا المرض و في نفس + +438 +00:31:12,320 --> 00:31:15,200 +الوقت ب simple procedures ممكن نعالج كمان ايه + +439 +00:31:15,200 --> 00:31:18,540 +المرض لذلك التواعي انا دايما بقول التواعي يجب ان + +440 +00:31:18,540 --> 00:31:22,260 +يستثمر فيها في كل المجتمع يجب ان يستثمر في التواعي + +441 +00:31:22,260 --> 00:31:26,000 +و التواعي الحقيقية المبنية على معلومات من مصادر + +442 +00:31:26,000 --> 00:31:31,200 +موثوقة و ليس من مصادر من ال internet بتعطيكم مثال + +443 +00:31:31,200 --> 00:31:35,380 +على مصدر غير موثوق فاكرين صرا ان في influenza ال + +444 +00:31:35,380 --> 00:31:38,590 +..الطيور او الخنازير الله ماتحق بالظبط لإن هو + +445 +00:31:38,590 --> 00:31:41,870 +الأخطر .. الأخطر كانت الطيور و هي على فكرة في + +446 +00:31:41,870 --> 00:31:46,330 +حالات تجينا من مصر وفيات ب influence الطيور لإن + +447 +00:31:46,330 --> 00:31:48,830 +اقولكوا ال influence الطيور أخطر بكثير من ال + +448 +00:31:48,830 --> 00:31:54,170 +influence الأخر كانت فكان في حد هكذا بعد ال + +449 +00:31:54,170 --> 00:31:57,850 +internet انه to protect yourself اشرب يانسون، + +450 +00:31:57,850 --> 00:31:59,250 +اليانسون فقد من البلد + +451 +00:32:02,600 --> 00:32:06,820 +في واحد كتب قرأ برضه حاجة موظف عنده في الجامعة + +452 +00:32:06,820 --> 00:32:11,760 +استخدمت مادة كيميائية مش فاكر بالظبط شو هي بس يعني + +453 +00:32:11,760 --> 00:32:15,580 +دوّبها في مياه و اعطاها لأبناء اطفال بناء على + +454 +00:32:15,580 --> 00:32:19,260 +معلومات من وين و هو موظف و هو متعلم يعني متعلم مش + +455 +00:32:19,260 --> 00:32:23,160 +في مجالنا مش في مجال العلوم الطبية ولكن في الآخر + +456 +00:32:23,160 --> 00:32:30,210 +استقاء المعلومات من مصادر موثوقة او منيعني أهم من + +457 +00:32:30,210 --> 00:32:32,850 +المعلومة نفسها، صح ولا لأ؟ تخيل .. تخيل إنه ناخد + +458 +00:32:32,850 --> 00:32:35,970 +المعلومة مهما كانت روايتها لكن في الآخر تطلع غلط + +459 +00:32:35,970 --> 00:32:41,630 +وممكن تكون مضرة وبالتالي لأ مطلوب مننا أنه نكون + +460 +00:32:41,630 --> 00:32:48,510 +يعني حذرين ما ننقل لبعض معلومات دون التوفق منها زي + +461 +00:32:48,510 --> 00:32:51,950 +حكاية العلاج بالأدوية بال antibiotics واحد بيقول + +462 +00:32:51,950 --> 00:32:54,510 +للتاني بيقول له والله أنا عندي مثلا احتقان في + +463 +00:32:54,510 --> 00:32:58,080 +الظهر وكده قبلهاقولوله أنا ماتجيب الفترة في نفس + +464 +00:32:58,080 --> 00:33:02,860 +الظروف و الدكتور وصفلي أنت بقتك حب زرجة و خضرة و + +465 +00:33:02,860 --> 00:33:07,100 +أخدت نص ال course و شوفيت .. و نص ال course بتاعي + +466 +00:33:07,100 --> 00:33:09,540 +ضايق المولد عندى، دي بليا، و كل واحد دي .. دي + +467 +00:33:09,540 --> 00:33:16,120 +بليا، المهم انه بيقول انه الوعي .. الوعي مهم كتير + +468 +00:33:16,680 --> 00:33:21,340 +في ارتقاء المجتمعات وبالتالي احنا عندنا بعض العدد + +469 +00:33:21,340 --> 00:33:23,980 +.. انا بقولش العدد كلها .. بعض العدد السيئة في هذا + +470 +00:33:23,980 --> 00:33:28,340 +المجموع يعني ان احنا كناس مثقفين ومتعلمين .. انه + +471 +00:33:28,340 --> 00:33:33,660 +ننشر ثقافة .. انه لأ في جهات + +472 +00:33:33,660 --> 00:33:37,420 +مرجعية للاشياء اللي متعلقة بالطب .. انا لما بدروح + +473 +00:33:37,420 --> 00:33:40,560 +استفتي في مسألة دينية .. بقول هنا الشيخ .. باستفتي + +474 +00:33:40,560 --> 00:33:43,440 +.. هنا مفتي الجهة .. باستفتي .. لما باستفتي شيء ب + +475 +00:33:43,440 --> 00:33:49,090 +.. ب .. ب .. بأمر بخص صحعشان بعض الأمور طبيعية لكن + +476 +00:33:49,090 --> 00:33:52,390 +مش طبيعية في النهاية لبس تفتي منى الطبيب كمان كان + +477 +00:33:52,390 --> 00:33:57,030 +ليه او لأولادي او لعيلتي لبس دايما نطلب الفتوى او + +478 +00:33:57,030 --> 00:34:02,230 +نطلب المعلومة من مصدرها الصحيح على الرغم من انفجار + +479 +00:34:02,230 --> 00:34:04,550 +المعلومات اللى متوفر لدينا على الشبكات الانترنت + +480 +00:34:04,550 --> 00:34:08,830 +اللى انه اكتب المصادر مضروبة مش صحيحة مش دقيقة + +481 +00:34:08,830 --> 00:34:12,890 +واحد بكتبانها بالظبط، بكتب، بحط، و للأسف احنا + +482 +00:34:12,890 --> 00:34:16,250 +مشهورين بال copy and paste، العالم العربي مشهورين، + +483 +00:34:16,250 --> 00:34:20,450 +بنلاقي نفس المعلومة على مئة منتدى، نفسها، منسوخة و + +484 +00:34:20,450 --> 00:34:24,170 +محطوطة، فمسكين المواطن بتطلع على المقال بجدها + +485 +00:34:24,170 --> 00:34:26,810 +انتقرها بيها مائة ألف مرة، فبقول الله يعني معلومة + +486 +00:34:26,810 --> 00:34:28,110 +حقيقية، مكتوبة في ال internet + +487 +00:34:31,620 --> 00:34:35,760 +تفقنا على أن التوعية مهمة جدا في عملية الخفض تبع + +488 +00:34:35,760 --> 00:34:41,200 +الوافيات باستخدام اللي هو المحاليل أو تعويق + +489 +00:34:41,200 --> 00:34:43,300 +الاسرائيل اللي بيفقدها المرض + +490 +00:34:46,100 --> 00:34:48,740 +شايفين الصورة هذه اللي .. اللي .. بيبدو انها من + +491 +00:34:48,740 --> 00:34:51,800 +جورنال فرنسي، تعرف الصورة اللي فوق هذا اللي طيروا + +492 +00:34:51,800 --> 00:34:56,400 +معاه عصايا و فيها منجل من تحت، هذا حصاد الأرواح، + +493 +00:34:56,400 --> 00:35:01,300 +هذا حصاد .. هذا .. هذا الشكل اللي هو ال death أو + +494 +00:35:01,300 --> 00:35:04,180 +اللي بيجلب الموت، اللي بيجلب الموت، ال cholera + +495 +00:35:04,180 --> 00:35:08,790 +كانت توصف بإن بيعدى شكلإنه ملك الموت جاي و معاه + +496 +00:35:08,790 --> 00:35:12,210 +اللي هو ال .. بتقطع الرؤوس هذي و يبمره على + +497 +00:35:12,210 --> 00:35:15,370 +المجتمعات اللي بيقطع .. بضرب فيهم اللي بيموت .. + +498 +00:35:15,370 --> 00:35:18,130 +موت اللي ميموتش .. ب .. ب .. طلعوا الصورة اللي على + +499 +00:35:18,130 --> 00:35:20,970 +الجانب تاني شوفوا ال .. dehydration شايفين إنتوا + +500 +00:35:20,970 --> 00:35:25,690 +الصورة هذه؟ شايفينها كويس إنتوا؟ في ال .. في ال .. + +501 +00:35:25,690 --> 00:35:28,570 +في ال .. في ال .. إذا حاطبتوا المحاضرة بعد التسجيل + +502 +00:35:28,570 --> 00:35:30,750 +تبعها هتشوفوا لإن هو .. هتشوفوها من ال .. من ال .. + +503 +00:35:30,750 --> 00:35:34,790 +من الكمبيوتر الجلدفاكرين في رمضان كيف كنت تعمل و + +504 +00:35:34,790 --> 00:35:38,070 +كيف صايمين و مش صايمين؟ تمام؟ طبعا كلكوا كنتوا + +505 +00:35:38,070 --> 00:35:44,970 +تكونوا صايمين، هي قد اللي راجف، من ايه؟ الجلدفلما + +506 +00:35:44,970 --> 00:35:49,330 +بيكون فيه جفاف غالبا الجلد will withstand يعني + +507 +00:35:49,330 --> 00:35:54,930 +بيظله واقف بشكل لفترة أطول لكن لما بيكون ال fluid + +508 +00:35:54,930 --> 00:35:58,690 +فيه بشكل جيد ع طول بيرجع normal زي انا كان فواضح + +509 +00:35:58,690 --> 00:36:02,150 +من مسكة الطفل هنا ان الجلد تبعه ان هو مصاب بال + +510 +00:36:02,150 --> 00:36:05,290 +dehydration بال dehydration طبعا ال goods اللي + +511 +00:36:05,290 --> 00:36:07,730 +بحكي هنا ال dehydration and death severe loss of + +512 +00:36:07,730 --> 00:36:13,330 +ions andال immunity strong immunity after recovery + +513 +00:36:13,330 --> 00:36:18,550 +و بتكون نتيجة عن اللي هو ال soluble immunoglobulin + +514 +00:36:18,550 --> 00:36:22,390 +A يعني بتعطينا immunity جيدة انا قلت strong + +515 +00:36:22,390 --> 00:36:26,070 +immunity لكن مش معناه تلحقينا long immunity برضه + +516 +00:36:26,070 --> 00:36:31,230 +كمان يعني لن تدوم اكتر من سنتين لن تدوم اكتر من + +517 +00:36:31,230 --> 00:36:38,720 +سنتين بحد اقصى ال pathological diagnosisSpacements + +518 +00:36:38,720 --> 00:36:42,440 +Stool Vomitus الستول طبعا اللي هو المعروف البراز + +519 +00:36:42,440 --> 00:36:46,660 +وال Vomitus اللي هو القيمة Stained Smear مايلها أي + +520 +00:36:46,660 --> 00:36:51,460 +قيمة إلا الحاجة واحدة Stained Smear من العينتين + +521 +00:36:51,460 --> 00:36:55,900 +نعمل Grams تاني نعمل Grams تاني انه نشوف ال curved + +522 +00:36:55,900 --> 00:36:59,560 +بس ال lie نشوف ال curved بس ال lie و ال curved بس + +523 +00:36:59,560 --> 00:37:04,320 +ال lie وجودها ليعني بالضرورةولود كوليرة وعدم + +524 +00:37:04,320 --> 00:37:08,280 +وجودها أيضا لا يعني بالضرورة إنها غير موجودة يبقى + +525 +00:37:08,280 --> 00:37:13,280 +معنا الحقيقية مش كتير له فائدة كبيرة الصحيح لكن + +526 +00:37:13,280 --> 00:37:16,080 +كانوا يعملوه الاستئناس في فترة الأوبيا كانوا يعملو + +527 +00:37:16,080 --> 00:37:21,060 +الاستئناس فقط لها ال culture هي الأهم على الإطلاق + +528 +00:37:21,060 --> 00:37:25,280 +عشان نجي نتعرف على ال microbe و نفحص مدى حساسيته + +529 +00:37:25,280 --> 00:37:29,340 +لل antibiotics في بيتنا نزرعه و المزرعة فيه نواسط + +530 +00:37:29,340 --> 00:37:32,120 +اسمه ال T-CBS إذا قلنا كوليرة + +531 +00:37:37,970 --> 00:37:42,590 +Cholera alkaline TCBS ايش ال TCBS؟ Thyosulfate + +532 +00:37:42,590 --> 00:37:51,210 +اكتبوا الاسم Thyosulfate citrate citrate + +533 +00:37:51,210 --> 00:37:56,930 +by salt sucrose + +534 +00:37:56,930 --> 00:38:02,370 +هذا الاسم مش لل salt لل sucrose مين + +535 +00:38:02,370 --> 00:38:05,410 +يقعد ليها مرة تانية؟ + +536 +00:38:11,150 --> 00:38:17,490 +هذا برضه الوصفة تعتبر مثال على selective and + +537 +00:38:17,490 --> 00:38:22,470 +differential selective and differential كتير + +538 +00:38:22,470 --> 00:38:25,430 +مره بعينة selective and differential دلوقت مكونكي + +539 +00:38:25,430 --> 00:38:30,350 +مكونكي و ال metal salt agar تمام وهي عندنا ال TCBS + +540 +00:38:30,350 --> 00:38:34,870 +طبعا احد الصفات تبعت من ال selective انه alkaline + +541 +00:38:34,870 --> 00:38:41,820 +pH طب كيف صار .. كيف صار ..differential من + +542 +00:38:41,820 --> 00:38:46,240 +الساكروز واضح من الساكروز دلوقت ايش الساكروز بتقول + +543 +00:38:46,240 --> 00:38:51,680 +بيقولوا انه البكتيريا اذا خمرت الساكروز ففيه عندنا + +544 +00:38:51,680 --> 00:38:57,540 +وسط او a pH indicator اللي .. اللي .. اللي بيخلي + +545 +00:38:57,540 --> 00:39:03,100 +الوسط لونه أخضر بيخلي الوسط لونه أخضر الآن ايش ال + +546 +00:39:03,100 --> 00:39:06,720 +.. ايش ال اسم ال .. ال .. ال indicator اللي في + +547 +00:39:06,720 --> 00:39:14,140 +السيطرةbromothymol blue عشانك + +548 +00:39:14,140 --> 00:39:19,120 +ال citrate اتحولت من الأخضر إلى أزرق نفسه هو اللي + +549 +00:39:19,120 --> 00:39:23,620 +احنا بنستخدمه bromothymol blue تركزوا معي اللي انا + +550 +00:39:23,620 --> 00:39:27,880 +اقوله نمت البكتيريا على الطبق هذا وعملت + +551 +00:39:27,880 --> 00:39:31,640 +fermentation لل ساكروس حياتي فايد بيقعدش ولا + +552 +00:39:31,640 --> 00:39:37,440 +هنقفل؟ هنقفل اللون الأصل اللون الأصل اللي وطن أخضر + +553 +00:39:38,160 --> 00:39:41,640 +لحظوا في ال citrate لما طلع فوق صار alkaline صار + +554 +00:39:41,640 --> 00:39:48,040 +أزرج لكن لو نزل تحت بصير أصفر بصير أصفر تحلج + +555 +00:39:48,040 --> 00:39:53,440 +المستعمرات تبعتنا تبعت ال CBS مستعمرات صفراء محاطة + +556 +00:39:53,440 --> 00:39:57,440 +بمنطقة صفراء as a result of sucrose fermentation + +557 +00:40:10,450 --> 00:40:16,330 +هذا الكلام على فكرة أنا قلته مستعمرة صفراء محاطة + +558 +00:40:16,330 --> 00:40:21,090 +بمنطقة صفراء as a result of Manitou fermentation + +559 +00:40:21,090 --> 00:40:25,590 +اختلف ال media و أنا قلتلك الوصف دايما المستعمر + +560 +00:40:25,590 --> 00:40:29,610 +يبقى أن يكون مرتبط بمين؟ الوصف المستعمر مرتبط بنوع + +561 +00:40:29,610 --> 00:40:35,250 +الوطن نفسهطبعا ال .. ال quick immunological method + +562 +00:40:35,250 --> 00:40:37,010 +انا شنفته المرة الماضية اللي هو ال + +563 +00:40:37,010 --> 00:40:39,430 +immunofluorescent و ال immunofluorescent انه انا + +564 +00:40:39,430 --> 00:40:43,770 +اضع شريحة .. انا اضع بكتيريا على شريحة و اعملها + +565 +00:40:43,770 --> 00:40:47,930 +fixation و اضيف عليها fluorescent antibody و بعدين + +566 +00:40:47,930 --> 00:40:51,690 +اغسل بعد الغسيل بنطلع على ال fluorescent + +567 +00:40:51,690 --> 00:40:54,730 +microscopy ال fluorescent microscopy عبارة عن + +568 +00:40:54,730 --> 00:40:58,150 +ultraviolet light تسقط على الشريحةفيه fluorescent + +569 +00:40:58,150 --> 00:41:02,610 +بيمطصها وبيشعلنا visible light نقدر نشوفه بالعين + +570 +00:41:02,610 --> 00:41:05,330 +تبعتنا، إذا نجينا visible light يعني فيه + +571 +00:41:05,330 --> 00:41:07,950 +fluorescent ليش ال fluorescent ضائل؟ لأنه فيه + +572 +00:41:07,950 --> 00:41:11,470 +antibody، ليش ال antibody ضائل؟ لأنه فيه بكتيريا، + +573 +00:41:11,470 --> 00:41:15,360 +ماشوفنا visible light، مافيش fluorescentطب انا حاط + +574 +00:41:15,360 --> 00:41:18,100 +فلوريسن راح مع الغسيل، ليش راح مع الغسيل؟ لإنه + +575 +00:41:18,100 --> 00:41:21,780 +مرتبط بالبكتيريا، مافيش بكتير البحث نيجد، تقنية + +576 +00:41:21,780 --> 00:41:24,960 +كتير ممتازة وسهلة، لكن شوية expensive لإن ال + +577 +00:41:24,960 --> 00:41:28,180 +immunofluorescent agent نفسها غالية، ال microscope + +578 +00:41:28,180 --> 00:41:31,080 +نفسه يعني تكلفته حوالي من تمان تلاف ليه عشان تلاف + +579 +00:41:31,080 --> 00:41:33,920 +دولار حسب الجودة، ممكن يوصل لعشرين ألف دولار كمان + +580 +00:41:33,920 --> 00:41:40,290 +بعض الميكروسكوبات المزودة بحواسيب وكمبيوتراتأعتقد + +581 +00:41:40,290 --> 00:41:43,470 +انه ال Vibrio prevention and control improved + +582 +00:41:43,470 --> 00:41:46,670 +sanitation لازم نحسم النظافة العامة بشكل عام في + +583 +00:41:46,670 --> 00:41:49,730 +البلد fluid and electrolyte replacement أنا مكبرها + +584 +00:41:49,730 --> 00:41:52,990 +في خط كبير عشان أقولكوا انه جزء مهم جدا من العلاج + +585 +00:41:52,990 --> 00:41:54,970 +وهذا ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +586 +00:41:54,970 --> 00:41:55,250 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +587 +00:41:55,250 --> 00:41:55,370 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +588 +00:41:55,370 --> 00:41:55,670 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +589 +00:41:55,670 --> 00:41:55,750 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +590 +00:41:55,750 --> 00:41:56,310 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +591 +00:41:56,310 --> 00:41:57,070 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +592 +00:41:57,070 --> 00:42:00,870 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +593 +00:42:00,870 --> 00:42:07,050 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +594 +00:42:08,430 --> 00:42:11,610 +كمان احنا كنا قبل شويه .. اي .. اي .. اي شئ متعلق + +595 +00:42:11,610 --> 00:42:14,310 +بنقله اهل الفكرة الأورال بدنا نشوف ال food + +596 +00:42:14,310 --> 00:42:17,410 +handlers لإن ال food handlers قد يكونوا carriers + +597 +00:42:17,410 --> 00:42:20,010 +.. قد يكونوا carriers ل .. ل .. ل .. ل .. ل .. ل + +598 +00:42:20,010 --> 00:42:20,170 +.. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل + +599 +00:42:20,170 --> 00:42:20,410 +.. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل + +600 +00:42:20,410 --> 00:42:21,110 +.. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل + +601 +00:42:21,110 --> 00:42:21,990 +.. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل + +602 +00:42:21,990 --> 00:42:23,230 +.. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل + +603 +00:42:23,230 --> 00:42:23,230 +.. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل + +604 +00:42:23,230 --> 00:42:23,230 +.. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل .. ل + +605 +00:42:23,230 --> 00:42:24,550 +.. ل .. ل .. ل + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IMdvAWrllOc_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IMdvAWrllOc_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..0d5fc46457524afa3aadf53887ab90b2deb1e12f --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IMdvAWrllOc_raw.srt @@ -0,0 +1,2444 @@ +1 +00:00:20,670 --> 00:00:25,430 +بسم الله الرحمن الرحيم احنا ال .. انتهينا اخر مرة + +2 +00:00:25,430 --> 00:00:28,490 +لما تكلمنا عن قدرة ال .. الميكروبات على ال + +3 +00:00:28,490 --> 00:00:32,810 +intracellular existence و قسمنا البكتيريا لصينتين + +4 +00:00:32,810 --> 00:00:35,470 +و قلنا اللي هم الصينة اللي obligate intracellular + +5 +00:00:35,470 --> 00:00:39,950 +و التاني اللي هو ال faculty ده شفنا مجموعة من ال + +6 +00:00:39,950 --> 00:00:44,670 +tactics خلينا نسميها او ال .. ال .. ال .. ال .. ال + +7 +00:00:44,670 --> 00:00:46,150 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +8 +00:00:46,150 --> 00:00:46,550 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +9 +00:00:46,550 --> 00:00:46,770 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +10 +00:00:46,770 --> 00:00:46,870 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +11 +00:00:46,870 --> 00:00:47,150 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +12 +00:00:47,150 --> 00:00:48,270 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +13 +00:00:48,270 --> 00:00:53,340 +.. الاللي من خلالها البكتيريا استطعت أن تقاوم + +14 +00:00:53,340 --> 00:00:58,860 +الفاغوسايتك killing، إما على مرتباط لليزوزوم أو + +15 +00:00:58,860 --> 00:01:04,340 +كانت نفسها resistant لليزوزوم ال destruction، يعني + +16 +00:01:04,340 --> 00:01:08,180 +في مجموعة كبيرة من ال tactics، اللي هو فالكلمة + +17 +00:01:08,180 --> 00:01:10,780 +معاها موضوع مختلف شوية، اللي هو ال antigenic + +18 +00:01:10,780 --> 00:01:17,050 +heterogenicityكنا متفقين كنا متفقين على انه جهاز + +19 +00:01:17,050 --> 00:01:21,950 +المناعة لديه القدرة على التعرف على بعض المكونات + +20 +00:01:21,950 --> 00:01:26,530 +البكتيرية او الفيروسية او الطفيلية كانتجينات و من + +21 +00:01:26,530 --> 00:01:31,190 +ثم تكوين أجسام مضادة ليده الانتجينات وبالتالي في + +22 +00:01:31,190 --> 00:01:35,530 +المرة القادمة بصير فيه من السهل جدا على جهاز + +23 +00:01:35,530 --> 00:01:40,290 +المناعة انه يكافح على الميكروباتلكن بالنسبة + +24 +00:01:40,290 --> 00:01:43,310 +للبكتيريا .. لكن بالنسبة للبكتيريا ايضا لديها + +25 +00:01:43,310 --> 00:01:47,310 +tactics هي كمان برضه عندها المقدرة عليها تغيير الـ + +26 +00:01:47,310 --> 00:01:52,010 +Antigenic Variation وبالتالي كل مرة بتدخل برقم + +27 +00:01:52,010 --> 00:01:58,430 +جديد او بانتجين جديد، اذا هذه المقدرة طبعا تم + +28 +00:01:58,430 --> 00:02:01,230 +اثباتها مقدرة البكتيريا تغيير الانتجينية تم + +29 +00:02:01,230 --> 00:02:05,030 +اثباتها in vitro وبتوقع تكون برضه موجودة في وين؟ + +30 +00:02:05,030 --> 00:02:11,130 +في ال in vivoكل هذا to evade the immune system ولا + +31 +00:02:11,130 --> 00:02:16,170 +أريد أن أذكركم بمثال دراسناه بشكل واقعي ال .. ال + +32 +00:02:16,170 --> 00:02:20,190 +.. ال .. ال بالاي ال attachment بالاي تحت ال + +33 +00:02:20,190 --> 00:02:26,630 +نيسيريا جونوريةو قلنا لكوا انها ال main virulence + +34 +00:02:26,630 --> 00:02:30,310 +factor لان اول خطوة في ال infection كانت ال + +35 +00:02:30,310 --> 00:02:34,710 +attachment وهي في موقع اصلا في مكان في سيول جارفة + +36 +00:02:34,710 --> 00:02:37,810 +وبالتالي حتى تستطيع ان تعمل infection لازم تبقى + +37 +00:02:37,810 --> 00:02:41,370 +هناك فجسم الإنسان تنبه لنا الموضوع كأول أجسام + +38 +00:02:41,370 --> 00:02:46,350 +مضالمين لل بلاي لكن البكتيريا تنبهتواصلت برضه تعمل + +39 +00:02:46,350 --> 00:02:52,090 +أندرينيك باريجين للبيلات في نقطة أول اللوحة المهمة + +40 +00:02:52,090 --> 00:02:58,510 +اللي بدنا نركز عليها كثير من ال viral instructors + +41 +00:03:00,830 --> 00:03:03,590 +كثير من ال virulence factors بتكون هي نفسها ال + +42 +00:03:03,590 --> 00:03:07,130 +antigen لكن في نفس الوقت ليس بالضرورة أن ال + +43 +00:03:07,130 --> 00:03:12,170 +antigen يكون هو ال virulence factor كمرة أن كثير + +44 +00:03:12,170 --> 00:03:15,770 +من ال virulence factors بحد ذاتها هي ال antigen + +45 +00:03:15,770 --> 00:03:20,710 +لكن ليس كل virulence factor هو ال antigen أو ليس + +46 +00:03:20,710 --> 00:03:25,620 +كل antigen هو virulence factor بنفس المعنىالنقطة + +47 +00:03:25,620 --> 00:03:28,260 +الأخيرة في الpart of Genesis اللي هتكلم عنها اليوم + +48 +00:03:28,260 --> 00:03:31,780 +اللي هي ال requirement of iron برضه بتدرجع فيكوا + +49 +00:03:31,780 --> 00:03:35,140 +للسابق و أذكركم بإنه لما درسنا ال structure of + +50 +00:03:35,140 --> 00:03:39,720 +bacteria ما درسناها لكي نحفظ مجوناته و بس لما + +51 +00:03:39,720 --> 00:03:43,560 +اتكلمنا عن ال pigment قلنا أحد وضاعي في ال pigment + +52 +00:03:43,560 --> 00:03:48,620 +is iron bindingفتعرفي تطلع على iron prediction + +53 +00:03:48,620 --> 00:03:52,480 +هانميته في اللوحة اللي أمامنا هلاجي انه for the + +54 +00:03:52,480 --> 00:03:56,540 +host the iron metabolism denies pathogenic + +55 +00:03:56,540 --> 00:04:00,600 +bacteria an adequate source ايش يعني؟ denies يعني + +56 +00:04:00,600 --> 00:04:07,280 +بيحرم البكتيريا من مصدر كافي لل iron بالنسبة + +57 +00:04:07,280 --> 00:04:13,410 +للبكتيريا they have developed severalsystems او + +58 +00:04:13,410 --> 00:04:16,970 +mechanism by which they can obtain iron from the + +59 +00:04:16,970 --> 00:04:21,010 +host واحد منها بيسموه الاسم high affinity iron + +60 +00:04:21,010 --> 00:04:24,590 +assimilation system high affinity iron + +61 +00:04:24,590 --> 00:04:28,530 +assimilation system انا مابدي اخد في تفاصيل + +62 +00:04:28,530 --> 00:04:33,970 +biochemistry كتيرة لكن من اللوحة القادمة مركب واحد + +63 +00:04:33,970 --> 00:04:39,120 +اسمه siderophoresالسينيروفورز هي iron binding + +64 +00:04:39,120 --> 00:04:43,280 +compounds مولودة في البكتيريا، لما تطلقها + +65 +00:04:43,280 --> 00:04:45,760 +البكتيريا زي حكيته بالظبط ال pigment اللي حكينا + +66 +00:04:45,760 --> 00:04:48,860 +عنها، لما تطلقها البكتيريا وتصير مولودة مع ال + +67 +00:04:48,860 --> 00:04:53,720 +host، فترتبط بمين؟بالايرون وبالتالي هذا الايرون is + +68 +00:04:53,720 --> 00:04:57,400 +unavailable to the host لكن available لمن؟ + +69 +00:04:57,400 --> 00:05:01,360 +للبكتيريا يبقى بنجر نقول ان مقدرة البكتيريا على + +70 +00:05:01,360 --> 00:05:06,220 +الارتباط او ال uptake للايرون هي تعتبر violence + +71 +00:05:06,220 --> 00:05:11,360 +factor لان بتحرم مين؟بتحرم ال host طب ال host .. + +72 +00:05:11,360 --> 00:05:14,140 +ال iron إله .. إله أهمية لل host .. أكيد إله أهمية + +73 +00:05:14,140 --> 00:05:19,840 +لل host و أنا بتأكد .. أنا بتأكد إنه حرمان ال host + +74 +00:05:19,840 --> 00:05:23,100 +من ال iron هيضعف جهاز المناعة، يعني بيبقى + +75 +00:05:23,100 --> 00:05:28,890 +advantage لمين؟ لأ اللي هو ال .. بأكيدبعد اللوحة + +76 +00:05:28,890 --> 00:05:32,510 +اللي هو السابقة احنا بنكون أنهينا جزء كبير الصحيح + +77 +00:05:32,510 --> 00:05:38,970 +من البعض ال diseases في البكتيريا لكن ما زال لدينا + +78 +00:05:38,970 --> 00:05:44,290 +نقطة مهمة جدا على وهي ال immune system لأنه لما + +79 +00:05:44,290 --> 00:05:48,130 +اتكلمت عن ال interaction في ال disease قلنا فيهن + +80 +00:05:48,130 --> 00:05:54,770 +تلت عناصر قلنا العامل نفسه وهو معبر عن البعض + +81 +00:05:58,690 --> 00:06:02,330 +الـ Immune System، تلت أشياء مرتبطة وقلنا ضفنا + +82 +00:06:02,330 --> 00:06:04,830 +عليها بيضر عبرها، اللي هو ال portal of impact + +83 +00:06:04,830 --> 00:06:11,050 +اليوم بتبدأ ناقش بشكل سطحي تماما، يعني معناه أخر + +84 +00:06:11,050 --> 00:06:13,730 +لن ندخل في التفاصيل، لإن في مساق كامل اسمه + +85 +00:06:13,730 --> 00:06:16,950 +immunology، تدرسوه إن شاء الله في السنة القادمة + +86 +00:06:16,950 --> 00:06:21,110 +Immunity of the host، خليني أطلع على اللوحة باللون + +87 +00:06:21,110 --> 00:06:27,940 +عربيهناك نوعان من الخلايا المنعية اللى بتخرج من ال + +88 +00:06:27,940 --> 00:06:33,320 +born marrow و بنقدر نصنفهم على انهم two lineage، + +89 +00:06:33,320 --> 00:06:37,620 +lineage يعني خطين، خط الأول myeloid و الخط التانى + +90 +00:06:37,620 --> 00:06:40,910 +اللى هو ال limbمن ال myeloid بيطلع عندنا ال + +91 +00:06:40,910 --> 00:06:42,930 +erythrocytes و ال platelets و الصحيحة دول اللي + +92 +00:06:42,930 --> 00:06:47,350 +بنتبرهم مش احنا من جهاز المناعة لكن بيطلع منهم ال + +93 +00:06:47,350 --> 00:06:51,750 +neutrophil و ال macrophages، إذا ال neutrophil و + +94 +00:06:51,750 --> 00:06:56,370 +ال macrophages هم من ال myeloid tissue بينما ال + +95 +00:06:56,370 --> 00:06:59,550 +lymphoid tissue بيطلع عندنا حاجة اسمها natural + +96 +00:06:59,550 --> 00:07:03,510 +killer، natural killer و من اسمها يعني وظيفتها + +97 +00:07:03,510 --> 00:07:09,720 +الأبدية to kill cellsو بيطلع من عندنا منها نوعين + +98 +00:07:09,720 --> 00:07:15,820 +كمان مهمين جدا اسمهم T-cells و B-cells ال T-cells + +99 +00:07:15,820 --> 00:07:19,080 +طبعا برضه احنا مصنفينها هنا صنفين فقط اللي هم ال + +100 +00:07:19,080 --> 00:07:25,760 +CD8 و CD4 و ال CD8 و CD4 هم عبارة عن markers ايش + +101 +00:07:25,760 --> 00:07:29,580 +اني markers يعني moleculesنعم من خلال المتعارف على + +102 +00:07:29,580 --> 00:07:34,060 +الخلايا فاسمه حتى الاختصار اللي هو clusters of + +103 +00:07:34,060 --> 00:07:36,980 +differentiation CD معناها cluster of + +104 +00:07:36,980 --> 00:07:42,720 +differentiation ففيها مجموعة CD8 ومجموعة CD4 الآن + +105 +00:07:42,720 --> 00:07:49,760 +ال CD8 بينبتق عنها خلايا اسمها cytotoxic لحظة + +106 +00:07:49,760 --> 00:07:54,420 +cytotoxic T cells ايش وضيفتها هذه ال cytotoxic T + +107 +00:07:54,420 --> 00:07:59,150 +cells برضه قتل يبقى فيه natural killerمن الـ + +108 +00:07:59,150 --> 00:08:04,010 +lymphoid و الـ cytotoxic T cell من اللي هو ال .. + +109 +00:08:04,010 --> 00:08:08,450 +برضه من ال lymphoid من ال .. من ال lymphoid ال CD4 + +110 +00:08:08,450 --> 00:08:13,350 +بيطلع منها بناء تهيلبر 1 و تهيلبر 2 و تهيلبر 1 و + +111 +00:08:13,350 --> 00:08:19,050 +تهيلبر 2 بيعتبرواEssential أو الـ mastermind للـ + +112 +00:08:19,050 --> 00:08:22,050 +humoral immune response يعني هم اللي بيعملوا + +113 +00:08:22,050 --> 00:08:26,670 +orchestrated action في ال immune system لذلك لما + +114 +00:08:26,670 --> 00:08:30,850 +بتنصاب الخلايا هذه من قبل فيروس ال aids، ايش بصير + +115 +00:08:30,850 --> 00:08:40,010 +لهم المريض؟ بيفقد المناعة بحسب عرض الخلايا المصابة + +116 +00:08:40,010 --> 00:08:41,190 +فال CD4 + +117 +00:08:48,390 --> 00:09:04,630 +الـ B-cells هي المسؤولة + +118 +00:09:04,630 --> 00:09:09,770 +عن إنتاج الـ Antivirals طبعا هنشوف كمان لوحة بتوضح + +119 +00:09:09,770 --> 00:09:15,030 +هذا الكلب بشكل أكتر لكن الـ B-cells إذا اتطبق بيها + +120 +00:09:15,030 --> 00:09:22,660 +أنتجينspecific إلها وكأنه إشارة يلا بيسل انقسمي، + +121 +00:09:22,660 --> 00:09:27,760 +ابتبدأ تنقسم انقسامات، فاكرين كلمة سلالة؟ clone، + +122 +00:09:27,760 --> 00:09:31,700 +فبيطلع منها clone، ال clone هذا .. ال clone يعني + +123 +00:09:31,700 --> 00:09:34,860 +السلالة كله نسخ عن مين؟ عن ال original cell اللي + +124 +00:09:34,860 --> 00:09:40,790 +صار لها activationالكلون بنقسم لقسمين، قسم بنسميه + +125 +00:09:40,790 --> 00:09:45,470 +memory cells و هذا بنقوله انت خليك ما سيركاش أي + +126 +00:09:45,470 --> 00:09:49,590 +development خليك صاحي، لو السنة الجاية اللي بعديها + +127 +00:09:49,590 --> 00:09:53,710 +نفس ال antigen دخل، انت بتشوف شغلك، الوزير التاني + +128 +00:09:53,710 --> 00:09:58,690 +لأ بينضج إلى ما يسمى ب plasma cells، ال plasma + +129 +00:09:58,690 --> 00:10:02,490 +cells هي المنتجة من ال antibodies، إذا ال + +130 +00:10:02,490 --> 00:10:07,840 +antibodies تنتج من قبل plasma cellsأحنا اللي هو + +131 +00:10:07,840 --> 00:10:12,800 +هذه تلقيص سريع لكن هناخد شوية تفاصيل ان شاء اللهزي + +132 +00:10:12,800 --> 00:10:15,980 +في اللوحة التانية عندنا cells of the immune system + +133 +00:10:15,980 --> 00:10:20,020 +بقولنا myeloid و lymphoid في ال myeloid قسمناها + +134 +00:10:20,020 --> 00:10:24,480 +لقسمين granulocytic و monocytic ال granules اللي + +135 +00:10:24,480 --> 00:10:27,980 +فاكرينهم ال granulocytes اللي هم التلاتة ال + +136 +00:10:27,980 --> 00:10:33,300 +neutrophil و ال basophil و ال eosinophil طبعا صنفه + +137 +00:10:33,300 --> 00:10:40,040 +feel eosino او baso او neutro حسب ال acidity او ال + +138 +00:10:40,040 --> 00:10:45,350 +pH تبعها اللي بتدخلالـ monocytic في منها اللي هو + +139 +00:10:45,350 --> 00:10:48,930 +ال macro phage و ال .. ال .. ال Langerhans و ال + +140 +00:10:48,930 --> 00:10:53,170 +copper cells و ال dendritic cells و ال dendritic + +141 +00:10:53,170 --> 00:10:56,070 +cells، هتلا كلهم monocytic cells، يعني من ال mono + +142 +00:10:56,070 --> 00:11:00,470 +sites، من ال mono sitesبينما ال lymphoid ال + +143 +00:11:00,470 --> 00:11:05,750 +lymphoid cells بتقسم زي ما تشوفين الى T cells وB + +144 +00:11:05,750 --> 00:11:09,490 +cells وطبعا دندراتي cells ساعتين علي علامة استثمار + +145 +00:11:09,490 --> 00:11:13,770 +على انه يعني under investigation انها تكون تابعة + +146 +00:11:13,770 --> 00:11:17,990 +ال lymphoid cells و ال T cells زي ما حكينا منها + +147 +00:11:17,990 --> 00:11:22,030 +اللي هو ال cytotoxic T cell اللي هي ال CD8 او ال + +148 +00:11:22,030 --> 00:11:25,890 +helper cell اللي هي ال CD4 ال B cells زي ما حكينا + +149 +00:11:25,890 --> 00:11:28,190 +بينبثق عنها ال plasma cells + +150 +00:11:31,740 --> 00:11:37,980 +طيب ال immune system could be classified based on + +151 +00:11:37,980 --> 00:11:44,020 +many variables وحق ال specificity انه specific و + +152 +00:11:44,020 --> 00:11:49,200 +لا non specific يعني هل بيقوم بوظيفته لإن هو عارف + +153 +00:11:49,200 --> 00:11:52,920 +هذا ال antigen كيف بيشتغل و انا كيف بتتعامل معاه + +154 +00:11:52,920 --> 00:11:58,880 +ولاأي .. بشكل .. مش بشكل عجوز .. بشكل خلينا نسميه + +155 +00:11:58,880 --> 00:12:03,520 +غير متخصص .. غير متخصص .. دخل لسن .. اتعرفنا عليه + +156 +00:12:03,520 --> 00:12:06,160 +ان هو غريب .. انتهى الموضوع .. بنا نهاجمه .. كأي + +157 +00:12:06,160 --> 00:12:11,080 +إن المان كان .. أمام؟ و تحت ال specific و non + +158 +00:12:11,080 --> 00:12:14,660 +specific لجينا أنوانين .. humoral و similar .. + +159 +00:12:14,660 --> 00:12:18,420 +تعالوا نفهم ايش معنى humoral بالأول .. humoral + +160 +00:12:18,420 --> 00:12:22,670 +معناها سائل ..سائل يعني مناعة سائلة، إيش مناعة .. + +161 +00:12:22,670 --> 00:12:26,870 +مناعة سائلة؟ يعني غير خلوية، غير خلوية، مافيش فيها + +162 +00:12:26,870 --> 00:12:30,810 +خلايا ولا مافيها ممكن antibodies، ممكن complement، + +163 +00:12:30,810 --> 00:12:33,770 +ممكن proteins، non-proteins، component، لكن في + +164 +00:12:33,770 --> 00:12:37,310 +الآخر مافيش إيه؟ خلايا، إيش بيسميها؟ humoral + +165 +00:12:37,310 --> 00:12:42,150 +immuneالتانية cellular immunity طبعا واضح من الاسم + +166 +00:12:42,150 --> 00:12:47,170 +خلوية بالدراسة وقتها نقطة عشان موضوع التصنيفات بعض + +167 +00:12:47,170 --> 00:12:53,050 +الأحيان بيحطنا في قلب معين احنا لما بيصير عنا ردت + +168 +00:12:53,050 --> 00:12:58,910 +فعل مناعية هل بيصير عنا cellular immunity ولا he + +169 +00:12:58,910 --> 00:13:01,990 +ولا ال immunity يعني ممكن بمعنى اخر ان واحدة + +170 +00:13:01,990 --> 00:13:06,310 +لحالها تصير والتانية ماتصيرش غالبا التانين بيصيروا + +171 +00:13:16,720 --> 00:13:20,780 +نقسم البكتيريا إلى Intracellular و Extracellular + +172 +00:13:20,780 --> 00:13:23,960 +والإنتراسيلولار تسيبوكوا منها إنها أبلجات ولا + +173 +00:13:23,960 --> 00:13:30,360 +فاكولتات في الآخر هي إنتراسيلولار فالإكستراسيلولار + +174 +00:13:30,360 --> 00:13:34,440 +أهم component في درجة المناعة للهيومورالإنهاتي + +175 +00:13:34,440 --> 00:13:37,420 +وأهم component في الهيومورالإنهاتي الانتيبادي + +176 +00:13:37,420 --> 00:13:41,200 +تمام؟ لما تكون البكتيريا عياشة + +177 +00:13:45,730 --> 00:13:52,570 +أهم component هو ال cellular immunity + +178 +00:13:52,570 --> 00:13:57,190 +أو إليها اسم تاني cell-mediated immunity فاكرين + +179 +00:13:57,190 --> 00:14:03,430 +كلمة cytotoxic T cell و natural killer cell؟ هذولا + +180 +00:14:03,430 --> 00:14:08,490 +تنتهينا إيش معلومة دولة؟ طبقتله خلاياطب أي خلايا + +181 +00:14:08,490 --> 00:14:12,090 +بيقتلوا الخلايا المصابة يبقى لما البكتيريا تكون + +182 +00:14:12,090 --> 00:14:17,490 +جوا داخل الخلية تبعتنا أو الفيروس يكون فبنقدرش + +183 +00:14:17,490 --> 00:14:21,370 +نقتل البكتيريا فبنقتل مين احنا اللي هو الخلية + +184 +00:14:21,370 --> 00:14:25,030 +بالبكتيريا اللي فيها اذا هيك ال T cell او ال cell + +185 +00:14:25,030 --> 00:14:27,930 +ميجد ال immunity بتكون كان الموضوع اهم + +186 +00:14:32,150 --> 00:14:34,830 +الميزان اللى موجود قدامك وهذا على الصفورة أو على + +187 +00:14:34,830 --> 00:14:37,350 +اللوحة هو الميزان اللى حكينا فيه من بداية + +188 +00:14:37,350 --> 00:14:42,090 +المحاضرات ال immunity او ال immunity ال ال + +189 +00:14:42,090 --> 00:14:44,510 +violence اللى هو ال type of microorganism و ال + +190 +00:14:44,510 --> 00:14:50,450 +number بوراس معناها الجرعة او ال numberو هؤلاء + +191 +00:14:50,450 --> 00:14:53,570 +التلات عناصر هم اللي بيحددوا إما ال frequency of + +192 +00:14:53,570 --> 00:14:56,810 +disease أو حتى ال severity of disease، إيش يعني + +193 +00:14:56,810 --> 00:15:01,930 +frequency؟ نسبة حدوثه أو شدته، تمام؟ شدة المرضى، + +194 +00:15:01,930 --> 00:15:08,690 +لكن مرارا و تكرارا أنا أكيد على مفهوم مهم جدا، وهو + +195 +00:15:08,690 --> 00:15:13,430 +إن ال immunity لم نستطع قياسها بشكل دقيق لحد الآن، + +196 +00:15:13,430 --> 00:15:18,210 +وبالتالي هذه المعادلةيعني احنا مقدرش نحل محالها + +197 +00:15:18,210 --> 00:15:21,890 +أرقام غالبا مانحطش محالها أرقام نعوض فيها و نحصل + +198 +00:15:21,890 --> 00:15:26,110 +على نسبة معينة ولكن هي logical equation يعني + +199 +00:15:26,110 --> 00:15:30,470 +خلّيني أقول معادلة منطقية أكتر من ما هي حساب طيب + +200 +00:15:30,470 --> 00:15:34,710 +أهمية الجهاز المناعة بدا منطلق لها من جانبين طبعا + +201 +00:15:34,710 --> 00:15:38,870 +لما أقول أهمية جهاز المناعة أكيد تلقائيا بيتبادل + +202 +00:15:38,870 --> 00:15:42,890 +لدهن الدور الإيجابي أصلا لأالدور الإيجابي في انه + +203 +00:15:42,890 --> 00:15:48,810 +بيعملنا حماية من الغزاة ال invaders تمام؟ و بيعمل + +204 +00:15:48,810 --> 00:15:54,050 +حتى كمان قتل و destruction للخلايا اللي مش طبيعية، + +205 +00:15:54,050 --> 00:15:57,630 +يعني مثلا تكونت خلية سرطانية، بالك منين بيهاجمها + +206 +00:15:57,630 --> 00:16:01,070 +أو لا بيهاجمها؟ جهاز المناعة، تمام؟ فجهاز المناعة + +207 +00:16:01,070 --> 00:16:05,970 +إله دورين بيحارب الغزاة، و لو صار في خلايا غريبة + +208 +00:16:05,970 --> 00:16:10,210 +جوا، يعني غريبة بمعنى أخر، نموها مش طبيعي، برضه + +209 +00:16:10,210 --> 00:16:16,120 +بيقتلهالكن فيه كمان له تأثير ضعف، determinant، إيش + +210 +00:16:16,120 --> 00:16:21,400 +تأثيره الضعف؟ إنه ال inflammation الناجمة عن شغله + +211 +00:16:21,400 --> 00:16:26,420 +لما بيشتغلأحنا بيعملوا مبنى عنا في الجامعة، المبنى + +212 +00:16:26,420 --> 00:16:29,100 +كويس ولّا .. لكن احنا قاعدين في المحاضرة تسمع + +213 +00:16:29,100 --> 00:16:34,380 +الصوت المزعج، مين مزعج؟ بما أنه بيكون بشيء إيجابي، + +214 +00:16:34,380 --> 00:16:37,700 +نفس الجثة، جثة المناعة، هو بيعمل الشيء الإيجابي + +215 +00:16:37,700 --> 00:16:43,540 +تلعبه، قد يعني يسبب إنها discomfort، اللي أهم من + +216 +00:16:43,540 --> 00:16:50,080 +ذلك رقم اتنين، damage to selfطبعا ال damage to + +217 +00:16:50,080 --> 00:16:54,400 +self احنا بنعرف انه جهاز المناعم program انه يتعرف + +218 +00:16:54,400 --> 00:16:57,960 +على خلايانا علينها self و على ال others in a non + +219 +00:16:57,960 --> 00:17:03,440 +self و هذا الطبيعي كلنا الان يعني لو جهاز المناعم + +220 +00:17:03,440 --> 00:17:06,140 +ماعنوش هذه القدرة كان ولا واحد فينا جاعل في هذه + +221 +00:17:06,140 --> 00:17:06,680 +المحاضرة + +222 +00:17:09,360 --> 00:17:13,140 +طيب جهاز المناعة بيفشل في التمييز بين self و non + +223 +00:17:13,140 --> 00:17:16,660 +-self؟ نعم بيفشل، أيش الأسباب؟ ممكن تكون كتيرة، أه + +224 +00:17:16,660 --> 00:17:21,280 +يفتكر واحد منها إيه له علاقة بالmicrobiology، الآن + +225 +00:17:21,280 --> 00:17:26,900 +لو بكتيريا لبست، فاكرين الخروف، تمام؟ لو لبست + +226 +00:17:26,900 --> 00:17:32,040 +أنتيجينات أو عليها أنتيجينات شبيهة بأنتيجيناتنا + +227 +00:17:32,040 --> 00:17:36,900 +احنا و النفط العدل تلقالي، كلمة شبيهة مش مطابقة، + +228 +00:17:36,900 --> 00:17:41,450 +يعني أتنامش بوابعض مش نسخة عن بعض،الان جسم الإنسان + +229 +00:17:41,450 --> 00:17:44,890 +تعرف أو جهاز الملعق تعرف على ال antigen تبع + +230 +00:17:44,890 --> 00:17:49,450 +البكتيريا و كوله أجسام مضادة الأجسام مضادة للأسف + +231 +00:17:49,450 --> 00:17:53,670 +ال specificity تبعتها ما جدرت تتميز الفروقات اللي + +232 +00:17:53,670 --> 00:17:57,590 +بين الاتنين فعملة أدات من تبع البكتيريا وعملة أدات + +233 +00:17:57,590 --> 00:17:58,090 +من مامين + +234 +00:18:02,680 --> 00:18:06,300 +من ال antibodies بدل ما توجه ضد البكتيريا ساسمين + +235 +00:18:06,300 --> 00:18:11,360 +لعدد القلب طبعا هي بالنسبة للبكتيريا ميزة طبعا بدل + +236 +00:18:11,360 --> 00:18:15,160 +ما تضربوا عشرة واحد بدل ما بيكفي يعني بتتحملوا لكن + +237 +00:18:15,160 --> 00:18:20,020 +بالنسبة للجسم اكيد بينضر لذلك بتعرفوا انتوا + +238 +00:18:20,020 --> 00:18:24,440 +الاتهاب اللواز اللي هو tonsillitis scriptococcus + +239 +00:18:24,440 --> 00:18:31,940 +virus عندها الحيلة هذهفالطفل اللى بيصاب مرارا و + +240 +00:18:31,940 --> 00:18:37,040 +تكرارا بال tonsillitis .. كلكم بتسمعه انه بيحاول + +241 +00:18:37,040 --> 00:18:41,800 +ان أهله يعمله عملية إزالة لازم .. لكن اللى بتتكرر + +242 +00:18:41,800 --> 00:18:45,680 +عنده الإصابة بال tonsillitis قد يكون عمره 15 سنة + +243 +00:18:45,680 --> 00:18:50,780 +لكن قلبه عمره 80 سنةالمفاصلة تكون عمرها ستين سنة، + +244 +00:18:50,780 --> 00:18:54,460 +ليش؟ من ال attack لل antibodies .. طبعا ال attack + +245 +00:18:54,460 --> 00:18:57,280 +لل antibodies شو بيقدّي يا بنات؟ هو ارتباط ال .. + +246 +00:18:57,280 --> 00:18:59,720 +ال .. ال antibody بال .. بال .. بال .. بالمكان مش + +247 +00:18:59,720 --> 00:19:04,080 +كتير يعني مضر لكن الارتباط بيقدّي لل activation لل + +248 +00:19:04,080 --> 00:19:07,830 +complementوالكملة بتعمل destruction لأنسجة اللى + +249 +00:19:07,830 --> 00:19:11,210 +مرتبط عليها ال antibody فبصير فى destruction فبصير + +250 +00:19:11,210 --> 00:19:16,130 +فى destruction وبالتالي بيظهر الأثر لذلك حتى وإن + +251 +00:19:16,130 --> 00:19:19,050 +بلد التونسوليتس بقت صديقة إن احنا صغار يعني كل شهر + +252 +00:19:19,050 --> 00:19:23,410 +شهرين مصاب مرة و احنا صغار لكن ال frequency تبع + +253 +00:19:23,410 --> 00:19:27,890 +حين ميزيد بالفعل بتأدى الى مخاطر دسيمة واحدة + +254 +00:19:27,890 --> 00:19:31,150 +بيسموها ال complication او ال sequella اللى هى + +255 +00:19:31,150 --> 00:19:37,910 +immunological والسبب فيها مين؟صحيح البكتيريا سبب + +256 +00:19:37,910 --> 00:19:40,410 +triggering، لكن ال .. اللي .. اللي أنتج ال + +257 +00:19:40,410 --> 00:19:44,110 +antibody هو من بيسمنا، فالضرر من وين؟ من البيسمنا، + +258 +00:19:44,110 --> 00:19:48,610 +و هذا يعتبر برضه autoimmune، إذن هناك وقت .. انا + +259 +00:19:48,610 --> 00:19:51,950 +في كمان ضرر تالت مش مذكور على الصبورة، بس ده كانت + +260 +00:19:51,950 --> 00:19:55,930 +الكوية، لما تكون ردد الفعل بجهة بعدية الملاعق غير + +261 +00:19:55,930 --> 00:20:00,210 +متناسبة مع حجب ال antigen، حكينا فيها زي ال super + +262 +00:20:00,210 --> 00:20:06,080 +antigenزي ما احد يتخيل انه عنده دبابة على ايده و + +263 +00:20:06,080 --> 00:20:12,920 +روحته خب مسدس مثلا تمام؟ مناسبة لرد الفعل؟ هتحدث + +264 +00:20:12,920 --> 00:20:17,100 +ضرر في ضرر؟ هتحدث ضرر اذا لما تكون ال exaggerated + +265 +00:20:17,100 --> 00:20:20,120 +immune response بالفعل يكون في ضرر في كمان ضرر + +266 +00:20:20,120 --> 00:20:25,940 +رابع اللي هو الhypersensitivity واحد جرسة نحلةو + +267 +00:20:25,940 --> 00:20:29,140 +خلال .. خلال ربع ساعة و نصف ساعة اتنفخ و صار عنده + +268 +00:20:29,140 --> 00:20:33,200 +إحمرار و ممكن الدور الدموي يسلحن في ال failure و + +269 +00:20:33,200 --> 00:20:37,440 +يموت هذا اسمه immediate type hypersensitive هذا رد + +270 +00:20:37,440 --> 00:20:42,320 +فعل مناعية برضه جهاز المناعة مسئول عنها تمام؟ إذا + +271 +00:20:42,320 --> 00:20:46,420 +هناك ب .. ب .. بمقابل الفوائد اللي بنجنها من جهاز + +272 +00:20:46,420 --> 00:20:50,160 +المناعة أيضا هناك ايه؟ مضارب ممكن تحدث + +273 +00:20:55,080 --> 00:20:59,860 +تقسيمة تانية adaptive and innate immunity أنا بدأ + +274 +00:20:59,860 --> 00:21:03,540 +أقولكوا بيها بكلمة بسيطة ال innate مابتعلمش ال + +275 +00:21:03,540 --> 00:21:04,960 +adaptive بتعلم + +276 +00:21:07,140 --> 00:21:10,180 +ليش بتعلمش ال .. ال .. ال innate؟ لأنه ماعندوش + +277 +00:21:10,180 --> 00:21:13,440 +memory ماعندوش memory يعني زي واحد نقعد نشرحله + +278 +00:21:13,440 --> 00:21:16,980 +مائة مرة microbiology وماعندوش ذاكرة ونقوله بيدي + +279 +00:21:16,980 --> 00:21:21,200 +امتحان، بنجح؟ مانتحش ولا عمرو قدر ولا عصر .. يمكن + +280 +00:21:21,200 --> 00:21:23,600 +ينسى الفصل .. ينسى المكان اللي بيروي عليه في + +281 +00:21:23,600 --> 00:21:28,520 +المحاضرة أساسا، ماعندوش .. ماعندوش ذاكرة فجهاز + +282 +00:21:28,520 --> 00:21:31,660 +المناعة .. جهاز المناعة .. جهاز المناعة بيقسم إلى + +283 +00:21:31,660 --> 00:21:35,930 +قسمين هذا لا يعني أن أنا بقلل من قيمةال innate + +284 +00:21:35,930 --> 00:21:40,810 +immune system بيقول بتعلماش لأ لكن انا بقولكوا + +285 +00:21:40,810 --> 00:21:45,050 +بوصفلكوا حالته ان هو بالفعل مابتعلمش بينما ال + +286 +00:21:45,050 --> 00:21:48,070 +adaptive immune system لإنه في عنده memory اخر + +287 +00:21:48,070 --> 00:21:52,870 +واحدة تلاتينا development of memory معناه الحاجة + +288 +00:21:52,870 --> 00:21:57,230 +انه بتعلم زي ما قلنا قبل شويه احنا بنقسم الخلايا + +289 +00:21:57,230 --> 00:21:59,530 +خلايا بتشتغل بتتحول ل plasma cell وخلايا اخر تتحول + +290 +00:21:59,530 --> 00:22:04,500 +لميه ل memory سليف بيها بيتعلم وبيتذكروبالتالي لما + +291 +00:22:04,500 --> 00:22:08,500 +ال microbe تقول كده مرة احنا we are ready وكتير من + +292 +00:22:08,500 --> 00:22:11,920 +الأمراض المعدية اللي كانت زمان قاتلة أصبحت غير + +293 +00:22:11,920 --> 00:22:19,040 +قادرة أعطيكم بس مثال بسيط كانت + +294 +00:22:19,040 --> 00:22:24,220 +لما الحصب تمر في حارة .. حارة يعني منطقة تمام؟ + +295 +00:22:24,220 --> 00:22:29,910 +تقتل نصف الأطفال اللي مولودين في المنطقةمرة تدخل + +296 +00:22:29,910 --> 00:22:34,910 +على منطقة معينة ممكن تتصل فيها لعشرين او تلاتين في + +297 +00:22:34,910 --> 00:22:41,290 +المية لما ال smallpox كان يصيب منطقة كانت نسبة + +298 +00:22:41,290 --> 00:22:44,570 +القتل والدمار والتشوه اللي ناتج عنه بقوة كونه + +299 +00:22:44,570 --> 00:22:49,470 +عالية جدا كل هذه الأمراض مُنِعت مُنِعة أو شبه + +300 +00:22:49,470 --> 00:22:55,030 +مُنِعة بال vaccination ايش ال vaccination؟ memory + +301 +00:22:56,050 --> 00:23:00,610 +انا عرضت جهاز المناعة للانتيجين فكون memory + +302 +00:23:00,610 --> 00:23:04,290 +وبالتالي يمكن كل يوم بجي ادخل انا بيروسات حصبة + +303 +00:23:04,290 --> 00:23:12,850 +وماانصبناش لأن في عندنا من يكافح اللي هو الحصب نفس + +304 +00:23:12,850 --> 00:23:18,850 +الجثة هو ان انا في الآخر بعطي immunity و memory مع + +305 +00:23:18,850 --> 00:23:24,050 +ال immunity طيب ان انا اعمل برضه فروقات الأول + +306 +00:23:28,620 --> 00:23:34,280 +antigen dependent كل جملة بنحكيها تؤدي لورقها في + +307 +00:23:34,280 --> 00:23:37,220 +الآخر مادام antigen dependent تبقى ال memory نجحت + +308 +00:23:37,220 --> 00:23:44,720 +من هذه القصة ال lag period كونه عملية دكية يبقى + +309 +00:23:44,720 --> 00:23:48,240 +فيها عملية تخطيط فيها عملية تخطيط وعملية التخطيط + +310 +00:23:48,240 --> 00:23:53,140 +بتاخد budget انا لما باجي باخد action بقعد مثلا نص + +311 +00:23:53,140 --> 00:23:58,330 +ساعة ساعة بكتب جدول و بعدين ببجي أتكلملكن إذا أنا + +312 +00:23:58,330 --> 00:24:01,290 +جاهز، خلاص، انتهينا من الموضوع، نبدأ من البداية + +313 +00:24:01,290 --> 00:24:04,810 +جهاز المناعة الأولاني اللي هو الـ Adaptive مافيش + +314 +00:24:04,810 --> 00:24:09,110 +عنده أي فترة جاهز لأن هو مجهز لهذه العملية، مابدوش + +315 +00:24:09,110 --> 00:24:12,250 +يفهم، بس نفس، تمام؟ بينما الجهاز التاني بيفهم، + +316 +00:24:12,250 --> 00:24:16,590 +فلازم يفكر ويعمل إجراءاته ثاني، فبتالي في فترة + +317 +00:24:16,590 --> 00:24:19,890 +زمنية ما بين دخول الميكروف و بين ظهور اللي هو ال + +318 +00:24:19,890 --> 00:24:24,470 +effect تبع المناعة Antigenic specific within + +319 +00:24:24,470 --> 00:24:31,240 +development of memoryطيب نجي لمقارنة بين ال + +320 +00:24:31,240 --> 00:24:34,740 +adaptive و ال innate immune system بس المرة ها دى + +321 +00:24:34,740 --> 00:24:38,920 +بناء على العواصر المكوّنة لكل كلهما العواصر الأول + +322 +00:24:38,920 --> 00:24:42,340 +ال physical barriers barriers معناها حوادث فهو + +323 +00:24:42,340 --> 00:24:48,420 +حوادث فيزيائية skin, gut, villi اللي هي ال villi + +324 +00:24:48,420 --> 00:24:53,460 +الموجودة فيها في ال intestine ال lung و ال cilia + +325 +00:24:54,360 --> 00:24:59,580 +هذه كلها عناصر physical موجودة في ال innate immune + +326 +00:24:59,580 --> 00:25:05,720 +system طول عمرنا الجلد تبعنا بنجرح و بيغتأم طول + +327 +00:25:05,720 --> 00:25:11,680 +عمرنا جلدنا موجود و ممنوع البكتيريا تدخل جوا، اذا + +328 +00:25:11,680 --> 00:25:14,680 +كيف تدخل جوا البكتيريا؟ لما يصير فيه خدش + +329 +00:25:19,690 --> 00:25:24,130 +الجلد لبعض الأحيان مش شايفين إنه جرح لكن واقعي + +330 +00:25:24,130 --> 00:25:28,450 +الأمر هو صار مجروح لكن مش .. خلّيني أقول مصطلح + +331 +00:25:28,450 --> 00:25:33,450 +تاني مش intact skin يعني مش متكامل فال skin موجود + +332 +00:25:33,450 --> 00:25:36,550 +physical barrier مهم جدا في ال innate immune + +333 +00:25:36,550 --> 00:25:39,610 +system طبعا في عامل أخرى يعني في العالم اللي أنا + +334 +00:25:39,610 --> 00:25:43,030 +هتدرسوها إن شاء الله زي ال gastric acid على سبيل + +335 +00:25:43,030 --> 00:25:46,610 +المثال في أن الشمع اللي مرود في الأذن كمان يعتبر + +336 +00:25:47,150 --> 00:25:51,110 +Innate immunity، الـ adaptive immunity مافيها + +337 +00:25:51,110 --> 00:25:55,850 +physical barriers مافيها physical barriers، + +338 +00:25:55,850 --> 00:26:01,490 +soluble factors في ال innate immunity many soluble + +339 +00:26:01,490 --> 00:26:06,970 +أو many proteins و non-protein factors، بينما في + +340 +00:26:06,970 --> 00:26:10,800 +ال adaptive immunityفيه عندى ال immunoglobulins و + +341 +00:26:10,800 --> 00:26:15,600 +خاصة اللى ذكرناه ال IGA فاكرينه ال IGA اللى قولنا + +342 +00:26:15,600 --> 00:26:18,740 +سكراتوري او solvigol و بروح على ال secretions + +343 +00:26:18,740 --> 00:26:20,860 +بنلاقيه فى اللعاب بنلاقيه فى ال nose بنلاقيه فى ال + +344 +00:26:20,860 --> 00:26:23,980 +vagina بنلاقيه فى اناكن فيها mucous membrane فى + +345 +00:26:23,980 --> 00:26:26,680 +التراكيا بنلاقيه لما هتكلمنا عن البكتيريا اللى + +346 +00:26:26,680 --> 00:26:32,340 +تنتج IGA proteases طيب ال cells اللى موجودة فى + +347 +00:26:32,340 --> 00:26:36,320 +الجهازين واحد ال phagocytes و ال natural killer و + +348 +00:26:36,320 --> 00:26:40,730 +ال eosinophil و ال K cellsبينما في ال adaptive + +349 +00:26:40,730 --> 00:26:44,930 +immunity لاحظوا معتمدة علمية على T و B lymphocytes + +350 +00:26:44,930 --> 00:26:51,750 +إذا بالفعل ال adaptive immunity معتمدة على ال + +351 +00:26:51,750 --> 00:26:56,650 +immunoglobulins و على ال T و ال B cells إذا طب + +352 +00:26:56,650 --> 00:27:00,530 +تعرف تميز ال adaptive immunity إلى تسمان رئيسي + +353 +00:27:00,530 --> 00:27:00,770 +يعني + +354 +00:27:05,200 --> 00:27:08,900 +بتحاول تلتقط اشريشك و الله يلحظوا ان ال size of ال + +355 +00:27:08,900 --> 00:27:13,660 +macrofage ضخم بالنسبة للبكتيريا فتستطيع في حركة + +356 +00:27:13,660 --> 00:27:17,460 +واحدة التقال اكثر من بكتيريا فالها كفاءة يعني اللي + +357 +00:27:17,460 --> 00:27:20,500 +هم استطيعوا ان يقللوا من أهميتها في جهاز المناع + +358 +00:27:20,500 --> 00:27:23,960 +هذا + +359 +00:27:23,960 --> 00:27:30,620 +الصورة اللي قدامنا بدنا نطلع عليها و نشوف نقطة + +360 +00:27:30,620 --> 00:27:31,200 +كتير مهمة + +361 +00:27:34,510 --> 00:27:41,210 +يارا تتركز على الخطن الخلية المصفوف بشكل عربي، + +362 +00:27:41,210 --> 00:27:46,510 +فلنعرف إن ال blood flows in vessels، في أوايا، + +363 +00:27:46,510 --> 00:27:51,050 +سميها الأواعي الدموية، إما تكون شرقين أو أوردة أو + +364 +00:27:51,050 --> 00:27:57,030 +كبلارس اللي بتوصل بين التحكم، الآن لما بيكون في + +365 +00:27:57,030 --> 00:28:02,940 +stimuli، كلمة stimuliوكلمة .. خلّينا ما ده بصراحة + +366 +00:28:02,940 --> 00:28:05,780 +نتكلم عن infection نتكلم عن مصطلح تاني اسمه + +367 +00:28:05,780 --> 00:28:11,000 +inflammation بالعربي التلاني غالبا referred to as + +368 +00:28:11,000 --> 00:28:14,620 +التهابات و واحد عنده inflammation أو عنده عدوة + +369 +00:28:14,620 --> 00:28:18,080 +بنسميها التهابات لكن العدوة هي التهاب العدو هي + +370 +00:28:18,080 --> 00:28:21,720 +التهاب لأن ليس كل التهاب هو عدوفال inflammation .. + +371 +00:28:21,720 --> 00:28:24,460 +ال .. ال .. ال infection inflammation بس مش كل + +372 +00:28:24,460 --> 00:28:28,280 +inflammation هو .. فال inflammation هو response لل + +373 +00:28:28,280 --> 00:28:31,800 +.. من الجسم ل injury و كلمة injury ممكن تفهمها جرح + +374 +00:28:31,800 --> 00:28:35,880 +بالسكين ممكن لو احنا انكبنا على ماء سخنة هذا + +375 +00:28:35,880 --> 00:28:40,300 +injury لو احنا دخل بكتيريا او فيروسات في الجسم هذا + +376 +00:28:40,300 --> 00:28:45,120 +injury chemicals ممكن تكون يعني اي عامل يحدث ضرر + +377 +00:28:45,120 --> 00:28:50,520 +بجسم اللي بيكوّله استجابة بيسميها inflammationمن + +378 +00:28:50,520 --> 00:28:54,500 +ضمن ال inflammation بعض الأحيان ما يسمى بـ ال .. + +379 +00:28:54,500 --> 00:28:58,000 +ال .. ال .. ال .. ال acute phase proteins acute + +380 +00:28:58,000 --> 00:29:02,700 +phase proteins حيمور معانا ذكرها في .. لل .. ل .. + +381 +00:29:02,700 --> 00:29:06,940 +لطلبات سنة تانية لما .. نفتقى شوية سنة لما ندرسوا + +382 +00:29:06,940 --> 00:29:10,020 +المناعة هتعرفوا إيش هي ال acute phase protein ب .. + +383 +00:29:10,020 --> 00:29:14,240 +ب .. بتفاصيل كبيرة إن شاء الله لكن بناعه إنه كمان + +384 +00:29:14,240 --> 00:29:19,370 +فيها اسمها cytokines cytokines هي ال messengersو + +385 +00:29:19,370 --> 00:29:24,130 +انا بدى انبهجكوا لنقطة معينة، ممكن انا اكون ال + +386 +00:29:24,130 --> 00:29:27,950 +messengerer ان اقولك نالي عفو لانا، هاي ال + +387 +00:29:27,950 --> 00:29:30,890 +messengerer انه واصلك انت to activate another + +388 +00:29:30,890 --> 00:29:35,480 +cellsto produce another cytokine فالعملية معقدة + +389 +00:29:35,480 --> 00:29:38,340 +ليس عملية سهلة و كل يوم نكتشف .. مش كل يوم يا ربنا + +390 +00:29:38,340 --> 00:29:43,640 +في فهم .. في فهم يوميا لأ كل سنة نكتشف واحد او + +391 +00:29:43,640 --> 00:29:48,080 +اتنين و حتى على مستوى الخلايا حتى نعرف أنواع خلايا + +392 +00:29:48,080 --> 00:29:52,000 +ماكناش نعرفها من ال immune system ال immune system + +393 +00:29:52,000 --> 00:29:56,380 +قائم على .. قائم على messages و very specific + +394 +00:29:56,380 --> 00:30:01,020 +messagesأي خلل فيها .. أي خلل فيها بيضعم جهاز + +395 +00:30:01,020 --> 00:30:04,080 +الملعنة عشانك أنا قلتلكوا دائما و أبدا يصعب جهاز + +396 +00:30:04,080 --> 00:30:08,120 +.. قياس جهاز الملعنة إذا واحد مش فاهم الجهاز كله + +397 +00:30:08,120 --> 00:30:15,580 +كب دي جسو بيكون صعب طيب صار في injury ب .. ب .. ب + +398 +00:30:15,580 --> 00:30:19,860 +.. ب بكتيريا صار في cytokines طلعت و نالتني الآن + +399 +00:30:19,860 --> 00:30:24,060 +ال neutrophil اللي هو الموجودة في الدم + +400 +00:30:26,890 --> 00:30:31,010 +نفس الـ cytokine بيعمل حزمة of vasodilatation، + +401 +00:30:31,010 --> 00:30:40,160 +توسيع للأوائل الدمويةعشان تقدر حضرة الـ netrophil + +402 +00:30:40,160 --> 00:30:47,300 +انها تخرج من الدم الى الانسجة الانسجة اللى مصابة + +403 +00:30:47,300 --> 00:30:52,520 +بعملية بيسميها diabetesis، diabetesis اللى هى غروب + +404 +00:30:52,520 --> 00:30:56,760 +كمان مرة الفاجوه صاعد من الدم او ال blood vessels + +405 +00:30:56,760 --> 00:30:58,220 +الى الانسجة + +406 +00:31:03,270 --> 00:31:08,770 +كمان مارعش معناها الكلمة خروج ال fagocytes من ال + +407 +00:31:08,770 --> 00:31:13,390 +blood vessels إلى الأنسجة الموصة و طبعا خروجها مش + +408 +00:31:13,390 --> 00:31:21,230 +عشوائي و انما نتيجة اللي هو stimuli ال + +409 +00:31:21,230 --> 00:31:35,830 +adaptive immunity اللي سمنها تتعلم صح؟وهي specific + +410 +00:31:35,830 --> 00:31:40,810 +and is mediated either by antibody or lymphoid + +411 +00:31:40,810 --> 00:31:49,190 +cell it can be active or it can be passive مهارة + +412 +00:31:49,190 --> 00:31:56,490 +الماضية قلنالكوا لو واحد يعني تعرض للذغاء من ثابان + +413 +00:31:58,110 --> 00:32:02,770 +السم اللى موجود الان فى الدم مصل الدم مافيش عندي + +414 +00:32:02,770 --> 00:32:08,110 +وسيلة ان انا اعمله اه انجل دمه برا واشيل السم منه + +415 +00:32:08,110 --> 00:32:12,190 +فوسيلة الاولى اللى بنلجألها ان يكون عندنا أجسام + +416 +00:32:12,190 --> 00:32:15,550 +مضادة وكل دولة فى العالم بتبقى متعارفة على + +417 +00:32:15,550 --> 00:32:18,190 +التعابين اللى عندها الحياة و العجارب و العناكب + +418 +00:32:18,190 --> 00:32:24,890 +السامة وعملة antibodies جاهزة فى vialsالطريقة + +419 +00:32:24,890 --> 00:32:28,970 +لعلاج الإنسان المصاب بالذبابة ان you were thabab + +420 +00:32:28,970 --> 00:32:32,930 +إذا جينا نحصل الذباب الطبيب يتعرف عليه يعطيه ال + +421 +00:32:32,930 --> 00:32:36,970 +antivenom لإنه ال antibody specificفهنا بنعطي + +422 +00:32:36,970 --> 00:32:40,250 +antibiotism جهزة هذا passive ولا active؟ passive + +423 +00:32:40,250 --> 00:32:44,850 +انه انا ما .. ماخلطوش يطمحش اعطي جهزة البناء وانما + +424 +00:32:44,850 --> 00:32:50,030 +اعطته حاجة جاهزة لكن هذا حل سريع ووحيد في هذه + +425 +00:32:50,030 --> 00:32:52,790 +الحالة لانه انا بقدرش ان انا اصلا نعطيه vaccine + +426 +00:32:52,790 --> 00:33:00,070 +لانه اصلا بيكون قام بدور بينما لما اعطته انا + +427 +00:33:00,070 --> 00:33:02,990 +vaccine زي التركسويدز اللي حكيت عنه قبل شويه هذا + +428 +00:33:02,990 --> 00:33:07,140 +يتبع ليه ايش؟ active immuneأجربلكم يا أهل الأذهان + +429 +00:33:07,140 --> 00:33:11,340 +احنا مرات انا بكون طالع مع صديق و مراهين مشوار + +430 +00:33:11,340 --> 00:33:16,960 +بسيارة هو اللي سايك انا بكون relaxed خالص ولا + +431 +00:33:16,960 --> 00:33:21,100 +بتأثر ولا بانتبه للطرق و لو بدرجع مرة تانية على + +432 +00:33:21,100 --> 00:33:25,220 +نفس المكان مابعرفش ليش؟ لأنه مش انا اللي بدرت + +433 +00:33:25,220 --> 00:33:30,240 +الجهد فيه انا وصليني جاهز بس لو العكس انا اللي سجت + +434 +00:33:30,240 --> 00:33:33,820 +و روحت و عرفت كل المناطق اللي طلعت المرة اللي جايش + +435 +00:33:33,820 --> 00:33:39,410 +بيهابكون اتعلمت فال passive ال passive بكون + +436 +00:33:39,410 --> 00:33:43,330 +already جهز برا بينما ال active احنا بنعمل + +437 +00:33:43,330 --> 00:33:47,410 +challenging للشخص او للمريض ان يكون هو الأجسام + +438 +00:33:47,410 --> 00:33:57,510 +الموجودة طب و لو بكتيري + +439 +00:33:57,510 --> 00:34:02,720 +و لو طبيعي؟يعني .. يعني انت اللي بتتكلم عنه ال + +440 +00:34:02,720 --> 00:34:06,060 +vaccine طب لو احنا انصبنا ب .. ب .. ب .. ب فيروس + +441 +00:34:06,060 --> 00:34:09,900 +او ببكتيريا معينة مش برضه هي بمثابة ال antigen + +442 +00:34:09,900 --> 00:34:14,100 +اللي لازم نتعرف عليه و .. و يكولله بس الفرق في ال + +443 +00:34:14,100 --> 00:34:19,120 +vaccine انه نمرضش بينما في العدوة العادية بنمرض + +444 +00:34:19,120 --> 00:34:23,480 +اما في المحصلة النهائية انه تحت انت منكون منع و + +445 +00:34:23,480 --> 00:34:29,920 +تصورةأو Artificial Artificial بتكون بالـ Vaccine و + +446 +00:34:29,920 --> 00:34:35,260 +Natural بتكون بال Infection طيب اللوحة اللي مدننا + +447 +00:34:35,260 --> 00:34:41,260 +هذه يعني تلخيص لبعض ال pathogens زي البكتيريا و ال + +448 +00:34:41,260 --> 00:34:46,200 +viruses و foreign proteins و fungi كتير منخصة فانا + +449 +00:34:46,200 --> 00:34:50,540 +للأسف يعني مضطر انه استخدم الصبورة على أساس انه + +450 +00:34:50,540 --> 00:34:56,580 +نوضح بعض النقاط ان شاء الله المتعلقة بالـانه في + +451 +00:34:56,580 --> 00:35:00,540 +هنا منطقة خالية الصحية في البلاد اللي ممكن نتعرف + +452 +00:35:00,540 --> 00:35:04,840 +عليها فاكرين حد اسمه macrofage؟ ايش اسمناه المرة + +453 +00:35:04,840 --> 00:35:10,520 +الماضية هنا؟ wise + +454 +00:35:10,520 --> 00:35:14,960 +man الراجل الحكيم ليش اسمه الراجل الحكيم؟ لأنه هي + +455 +00:35:14,960 --> 00:35:20,740 +اللي بتعمل عملية phagocytosis وبعدين بتقرر لما + +456 +00:35:20,740 --> 00:35:26,330 +تكسر البكتيريا بتقررمين من الانتيجينات .. مين من + +457 +00:35:26,330 --> 00:35:30,490 +الانتيجينات تمام؟ اللي موجودة على أسطحة البكتيريا + +458 +00:35:30,490 --> 00:35:35,330 +أو الفيروس بتعمله presentation فبتمسك أنتيجين أو + +459 +00:35:35,330 --> 00:35:39,830 +أكثر و بتعملهم presentation على السطح هذه حركتها + +460 +00:35:39,830 --> 00:35:44,570 +بطيئة حركتها بطيئة فبطولة بتدور على مين .. عالي + +461 +00:35:44,570 --> 00:35:50,730 +حركتهم نجيطة .. مين حركتهم نجيطة؟ ال T cells الان + +462 +00:35:50,730 --> 00:35:54,980 +مش اي T cellأنا مش قدر أقول كل شيء specific في + +463 +00:35:54,980 --> 00:35:57,620 +جهاز الملعب، كل شيء specific، مافيش اي T cell + +464 +00:35:57,620 --> 00:36:03,540 +بتروح الماكروفاية على تجمعات T cells و بتطلع مين + +465 +00:36:03,540 --> 00:36:10,860 +عندها receptor بيقابل ال antigen تبعنا ممكن تحتك + +466 +00:36:10,860 --> 00:36:16,860 +فيهم و تقرأهم و في الآخر تقرر اي T cell + +467 +00:36:22,560 --> 00:36:26,900 +الـ Receptor أو عفوًا الـ Antigen representation + +468 +00:36:26,900 --> 00:36:35,680 +مناسب إليها بينتقل على طول هذا إلى الـ T cell الـ + +469 +00:36:35,680 --> 00:36:39,560 +T cell لنا خفيفة ونشيطة أسرع من مين؟ من ال macro + +470 +00:36:39,560 --> 00:36:47,180 +phage بتروح تدور على مين؟ على ال V cell المناسبة + +471 +00:36:47,180 --> 00:36:52,460 +يبقى ال macro phage أكلت البكتيرياعامة + +472 +00:36:52,460 --> 00:36:56,300 +الفيروساكتوز أو الفيروس أو whatever و عامة ما يسمى + +473 +00:36:56,300 --> 00:37:00,360 +كما مرّت Antigen Presentation اللي بيعرفش إيش هال + +474 +00:37:00,360 --> 00:37:02,740 +Antigen Presentation بتصفره على اللي يومين هدولة + +475 +00:37:02,740 --> 00:37:09,260 +فترة دقسة بس يكون الخلية بيسموها مرّات APC Antigen + +476 +00:37:09,260 --> 00:37:15,040 +Presenting Cell نظرا للدور العظيم المناطق دي هذه + +477 +00:37:15,040 --> 00:37:21,200 +الخلية من إظهار لل Antigenات تبعها البيتين بأصول + +478 +00:37:21,200 --> 00:37:25,690 +مين اللي احنا؟لل بي سيل ال بي سيل هي على الصبورة + +479 +00:37:25,690 --> 00:37:31,890 +اللوحة تاني بتطلع what is next ال بي سيل في هذه + +480 +00:37:31,890 --> 00:37:38,550 +الحالة اللي قبلته شاملة ال بي سيل تخيل واحد بينتظر + +481 +00:37:38,550 --> 00:37:43,330 +حدث مهم في حياته لن يقوم بيه إلا مرة واحدة وإيجاه + +482 +00:37:43,330 --> 00:37:50,130 +هذا الخبرارتباط الانتجن بال B-cell وكأنه Mitogen + +483 +00:37:50,130 --> 00:37:53,230 +Mitogen فاكرين انكي ميتوجين؟ بلحك ان انا اقول ان + +484 +00:37:53,230 --> 00:37:58,430 +ال Mitogen هو compound بيعمل stimulation لل cell + +485 +00:37:58,430 --> 00:38:03,430 +division فالانتجن بمجرد انه مرتبط بال B-cell تبدأ + +486 +00:38:03,430 --> 00:38:08,220 +ال B-cell تنقسممفتاح سامناه ووصفناه كإن هو يقول لا + +487 +00:38:08,220 --> 00:38:13,540 +وكأنه ماتش، إذا بدأت الخلية تنقسم وقلنا بتنقسم إلى + +488 +00:38:13,540 --> 00:38:17,360 +بي سيلز وإلى بي سيلز، لكن بي سيلز سمناهم memory + +489 +00:38:17,360 --> 00:38:23,740 +cells والتانية سمناهم لا، نظر الولالحظوا الحجم تبع + +490 +00:38:23,740 --> 00:38:28,560 +ال plasma cell أكبر بكثير من مين من ال B cells ال + +491 +00:38:28,560 --> 00:38:33,780 +memory cells حجمها بلايه بيضلوا نفس الحجم تبع اللي + +492 +00:38:33,780 --> 00:38:37,980 +هو ال B cells بينما ال plasma cell طبعا انها بدأت + +493 +00:38:37,980 --> 00:38:43,600 +تتكون بروتينات ال minoglobulins ال antibodies من + +494 +00:38:43,600 --> 00:38:51,370 +صنف واحدالـ B-cell الواحدة اللى اتكاثرت الى عشرات + +495 +00:38:51,370 --> 00:38:55,810 +او مئات كلها هتنتج نفس الصنف من مين من ال + +496 +00:38:55,810 --> 00:39:01,890 +antibodies لذلك B-clone, clonal, monoclonal تمام؟ + +497 +00:39:01,890 --> 00:39:05,310 +اذا اكتر من B-cell اللى اشتغلت ال response بيكون + +498 +00:39:05,310 --> 00:39:10,890 +polyclonal طب عندنا نقطة هذه على فكرة انه مهمة جدا + +499 +00:39:10,890 --> 00:39:14,350 +بالنسبة لطلابنا يعرفوها الفرق بين monoclonal و + +500 +00:39:14,350 --> 00:39:15,290 +polyclonal + +501 +00:39:18,740 --> 00:39:22,860 +طبعا التخيل اللي كنتوا عن الانتجين انه هو قطعة + +502 +00:39:22,860 --> 00:39:28,740 +واحدة صح؟ كبرنا اليوم احنا صارنا في الجامعة مش في + +503 +00:39:28,740 --> 00:39:33,720 +التنوية العامة لازم نعرف ان الانتجين الواحد قد + +504 +00:39:33,720 --> 00:39:38,300 +يكون او يحمل قد يحمل اكتر من ما يسمى Epitope + +505 +00:39:38,300 --> 00:39:43,580 +Epitope يعني بيسموه Antigenic determinants + +506 +00:39:43,580 --> 00:39:47,900 +الانتجين الواحد بيبقى فيه اكتر من Epitopeفاكتر من + +507 +00:39:47,900 --> 00:39:55,640 +ايه؟ من ابيتوب طيب كل ابيتوب بينشأله antibody مثل + +508 +00:39:55,640 --> 00:39:58,520 +الاندرجين الواحد بينشأه يعني لو الاندرجين الواحد + +509 +00:39:58,520 --> 00:40:04,260 +فيه أربع ابيتوب أكم antibody بيطلعله؟ أربع طيب لو + +510 +00:40:04,260 --> 00:40:08,140 +عندي محلول .. محلول في أربع أنواع من ال antibodies + +511 +00:40:08,140 --> 00:40:12,040 +بقدر أطلعله monoclonal يا بنات؟ لأ بقدر أطلعله + +512 +00:40:12,040 --> 00:40:16,890 +monoclonalلأن هو محتوي على أربع أصناف من ال + +513 +00:40:16,890 --> 00:40:20,950 +antibodies و كل من الأربع يانيبولي .. أربع + +514 +00:40:20,950 --> 00:40:25,730 +يانيبولي .. بس لما يكون صنف واحد بنسميه monoclonal + +515 +00:40:25,730 --> 00:40:30,390 +طب إيش أهمية ال monoclonal في حياتنا؟ احنا كتلاب + +516 +00:40:30,390 --> 00:40:34,590 +علوم طبية مخبرية، عارفين إن هذا أحدث ثورة في ال + +517 +00:40:34,590 --> 00:40:38,940 +diagnostics؟ال specificity ايش هي ال specificity + +518 +00:40:38,940 --> 00:40:44,300 +التخصصية ايش هي التخصصية يعني لو انا فعلت شيء مع + +519 +00:40:44,300 --> 00:40:48,860 +شيء الاصل if هذا specific مايتفعل الا مع الشيء + +520 +00:40:48,860 --> 00:40:55,420 +اللي بديه يعني صح هذا ال specificity طبعا احنا + +521 +00:40:55,420 --> 00:40:59,560 +بفترض ان ال antibody is highly specific صح عليها + +522 +00:40:59,560 --> 00:41:03,480 +علامة استفادة عليها علامة استفادة لكن لما يكونوا + +523 +00:41:03,480 --> 00:41:10,150 +اتنينو مش highly specific او تلاتة و مش highly + +524 +00:41:10,150 --> 00:41:15,730 +specific فرصة ما يسمى بال cross reaction انا رايح + +525 +00:41:15,730 --> 00:41:20,670 +ارتبط مع X ليش ارتبط مع Y؟ اذا ارتبط مع X و مع Y + +526 +00:41:20,670 --> 00:41:26,470 +بنسميها cross reaction تفاعل مش طبيعي، يعني تفاعل + +527 +00:41:26,470 --> 00:41:30,620 +مع اتنين، اقول تفاعل مع مين؟ مع واحدطيب إذا كان هو + +528 +00:41:30,620 --> 00:41:34,660 +antibody واحد، mono فرصة إنه يعمل cross reaction + +529 +00:41:34,660 --> 00:41:39,180 +بتكون أقل من لو كانوا اتنين، همام؟ وبالتالي ال + +530 +00:41:39,180 --> 00:41:41,880 +mono-clonal antibody أحدث ثورة في ال diagnostics، + +531 +00:41:41,880 --> 00:41:45,040 +الآن معظم الفحوصات ال immunology أو ال serology + +532 +00:41:45,040 --> 00:41:48,400 +معتمدة علينا نتكلم عن مين هنا؟ عن mono-clonal + +533 +00:41:48,400 --> 00:41:54,460 +antibody لكن لو بنعمل علاج زي اللي قلنا عنه muscle + +534 +00:41:54,460 --> 00:41:58,380 +جابه شوية، بتفضلوا mono-clonal ولا polyclonal؟ + +535 +00:41:59,560 --> 00:42:03,200 +Polyclonal انه يعمل على كل ال Epitopes الموجودة + +536 +00:42:03,200 --> 00:42:05,560 +على مين على الانتجن، يعني انا مش قصده انه .. انا + +537 +00:42:05,560 --> 00:42:09,860 +مابتدهاش ناخد انطباع ان ال monoclonal هو ال top و + +538 +00:42:09,860 --> 00:42:12,720 +ال polyclonal هو ايه، لأ ممكن نجيله برضه بعض + +539 +00:42:12,720 --> 00:42:17,500 +الاستخدام طبعا ال antibody اللي بينتج لايتفاعل + +540 +00:42:17,500 --> 00:42:22,680 +المفروض لايتفاعل الا مع ال antigen اللي احدث اللي + +541 +00:42:22,680 --> 00:42:27,050 +هو انتجهو هذا اللي احنا سميناها specificity، لكن + +542 +00:42:27,050 --> 00:42:30,010 +للأسف مش hundred percent specificity، وشوفتوا لو + +543 +00:42:30,010 --> 00:42:33,590 +بشوية انتوا انه ال .. ال .. ال .. البياز المناء + +544 +00:42:33,590 --> 00:42:37,670 +هاجم بالانتباه السبع للبكتيريا، وهاجم من كمان + +545 +00:42:37,670 --> 00:42:41,410 +عضلات القلب، يبقى only specificity او وين ال + +546 +00:42:41,410 --> 00:42:45,850 +absolute specificity مش موجودة، ال absolute، احنا + +547 +00:42:45,850 --> 00:42:48,450 +بنقول في نوع من ال specificity، لكن مش absolute + +548 +00:42:50,080 --> 00:42:54,600 +طيب، هذه الجملة اللوحة ذكرناها الصحيحة، لكن لا + +549 +00:42:54,600 --> 00:42:56,920 +يبقى أسئلة أذكركم فيها إن ال immunity لل + +550 +00:42:56,920 --> 00:43:00,420 +extracellular bacterial infection و ال immunity to + +551 +00:43:00,420 --> 00:43:06,460 +intracellular mainly مين الأولانية humoral ليها ال + +552 +00:43:06,460 --> 00:43:10,060 +antibodies بينما التانية ال mainly مين ال cell + +553 +00:43:10,060 --> 00:43:14,300 +mediated أو الcellular immune اسمينها cellular + +554 +00:43:14,300 --> 00:43:16,860 +immunity أو cell mediated immune + +555 +00:43:27,870 --> 00:43:35,390 +إيش يعني inadequate؟ غير كافي، غير كافي Causes + +556 +00:43:35,390 --> 00:43:40,790 +immune suppression An example is an infection with + +557 +00:43:40,790 --> 00:43:45,270 +HIV, which alters T cell immunity and allows + +558 +00:43:45,270 --> 00:43:49,320 +further infection with opportunistic pathogensيبقى + +559 +00:43:49,320 --> 00:43:54,060 +أحد .. أحد مظاهر اللي قلفناها ان ال immune system + +560 +00:43:54,060 --> 00:43:57,580 +بيكون inadequate لما يصاب الإنسان بفيروس ال aids + +561 +00:43:57,580 --> 00:44:02,460 +ففيروس ال aids بيشتغل على مين هنا؟ على ال CD4 على + +562 +00:44:02,460 --> 00:44:05,800 +ال CD4 اللي هي ال T helper cells اللي هي عبارة عن + +563 +00:44:05,800 --> 00:44:11,480 +T cells ولو تكون ال CD4 ال CD4 cells يعني هي اللي + +564 +00:44:11,480 --> 00:44:16,100 +بتعمل orchestrating لل immune system فبالتالي + +565 +00:44:18,010 --> 00:44:23,090 +مافيش الآن orchestrated immune response يبقى ال + +566 +00:44:23,090 --> 00:44:27,190 +opportunistic pathogens will cause infection لإنه + +567 +00:44:27,190 --> 00:44:29,150 +مرض ال aids بتفكر ان انت بيموت من ال .. من ال + +568 +00:44:29,150 --> 00:44:33,310 +virus سبب ال aids؟ لا هو بيموت من إصابة الأمراض + +569 +00:44:33,310 --> 00:44:36,490 +اللي هو خلاص انت يازر عنده انخفض تماما و هو بتالس + +570 +00:44:36,490 --> 00:44:41,120 +أصبح غرض للإصابةو يمكن كأني قلتلكوا أن مرض ال aids + +571 +00:44:41,120 --> 00:44:45,980 +زاد علينا علم ال microbiology كم كبير و زاد علم .. + +572 +00:44:45,980 --> 00:44:51,340 +فاهمنا علم المناعة بشكل كبير لإن كثير من + +573 +00:44:51,340 --> 00:44:53,840 +الميكروباط اللي كنا بيقول عنها non pathogen ثابت + +574 +00:44:53,840 --> 00:44:57,560 +انها بتعمل infection في مرض مريض ال aids فصلنا + +575 +00:44:57,560 --> 00:44:59,760 +اللي كنا بيقول عنه non pathogen اللي هو + +576 +00:44:59,760 --> 00:45:06,290 +opportunistic pathogen فهذه نقطة كتير مهمةطيب، + +577 +00:45:06,290 --> 00:45:09,530 +inadequate immune response في عندنا release of + +578 +00:45:09,530 --> 00:45:12,610 +toxins that function as super antigens، فاكرين ال + +579 +00:45:12,610 --> 00:45:16,490 +super antigen؟ الصورة هذه، حلوة الصورة اللي هناك، + +580 +00:45:16,490 --> 00:45:21,130 +فاهم؟ اللي فوق على الزاوية، فاهم شايفينها؟ هذا + +581 +00:45:21,130 --> 00:45:24,470 +عبارة عن super antigen، ال super antigen، فاكرين + +582 +00:45:24,470 --> 00:45:27,070 +ما عشان بدنا نرسم كده الرسم أخيرا؟ + +583 +00:45:33,380 --> 00:45:36,820 +لما كنا نتكلم عن ال macro phage ونتكلم عن ال + +584 +00:45:36,820 --> 00:45:41,740 +antigen presenting cells ومش قولنا إننا بنتحول ال + +585 +00:45:41,740 --> 00:45:46,980 +antigen هذا ل ال T-cells الآن يفترض إن ال T-cell + +586 +00:45:46,980 --> 00:45:51,860 +يكون + +587 +00:45:51,860 --> 00:45:56,600 +لها receptor مطابق عشان تقدر تاخد مين؟ ال antigen + +588 +00:45:56,600 --> 00:46:02,720 +هذا normal binding حطرة ال super antigenبيروح + +589 +00:46:02,720 --> 00:46:06,600 +بيخلّي خليتين و اتفج يان بعض و بيلازجون مع بعض زي + +590 +00:46:06,600 --> 00:46:13,100 +حاجة فو بيصيروا كانوا إشراقان مقنعوم، binding، هذا + +591 +00:46:13,100 --> 00:46:18,380 +بتبقى هو برضه binding، لكن binding specific؟ لأ مش + +592 +00:46:18,380 --> 00:46:23,480 +specific، وبالتالي هو أي خليتين إجراب من بعض بيروح + +593 +00:46:23,480 --> 00:46:27,110 +بيلازجون ببعضفبيعملهم binding فبصير لنا non + +594 +00:46:27,110 --> 00:46:33,210 +specific activation فكرين لما قلنا قبل دقائق ان + +595 +00:46:33,210 --> 00:46:36,910 +هذا ال non specific activation بيعمل ما يسمى بال + +596 +00:46:36,910 --> 00:46:41,050 +immunological storm عصفة مناعية cytokines خلايا + +597 +00:46:41,050 --> 00:46:45,830 +وبالتالي يمكن يصير لنا shock و الشك تؤدي الى + +598 +00:46:45,830 --> 00:46:51,070 +الوفاة فممكن تؤدي الى الوفاة في أن النقطة التالتة + +599 +00:46:51,070 --> 00:46:54,810 +اللي هي ال evadethe immune defenses by altering + +600 +00:46:54,810 --> 00:46:57,230 +their antigenic structure هذا ما حكيناه قبل كده + +601 +00:46:57,230 --> 00:47:00,950 +انه قد يخدع جهاز المناعة يعني المناعة هتكون ممتازة + +602 +00:47:00,950 --> 00:47:05,230 +مافي مشكلة فيها لكن لإنه ال .. ال .. الميكروب نفسه + +603 +00:47:05,230 --> 00:47:07,970 +عنده المقدر على انه يعمل ال vision هو بتاع يفشل + +604 +00:47:07,970 --> 00:47:11,490 +انه يجيب ال response الطبيعي تبعه المرة أخرى ان + +605 +00:47:11,490 --> 00:47:15,470 +شاء الله سنكمل باقى ال chapter وينعمل عملية ال + +606 +00:47:15,470 --> 00:47:19,990 +vision فيارات تكون يعني في تحضير لأسئلة محضرين + +607 +00:47:19,990 --> 00:47:23,760 +حاجة للنقاط اللي مش فاهمينها انتواوفي نفس الوقت + +608 +00:47:23,760 --> 00:47:27,940 +يكون في عنا يعني استعداد أنه نجيب على بعض الأسئلة، + +609 +00:47:27,940 --> 00:47:30,300 +يعني لو أنا سأل بعض الأسئلة، أنه نجاوب عليها، يعني + +610 +00:47:30,300 --> 00:47:34,920 +دراسة quiz، مش quiz، لكن يعني عشان ماتنحرجوش يعني، + +611 +00:47:34,920 --> 00:47:36,740 +نجف ومانعرفش نجاوب + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IzxXy5S0TBo.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IzxXy5S0TBo.srt new file mode 100644 index 0000000000000000000000000000000000000000..4435e1effa96cd9fb2f6474c9f27b81685b9480c --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IzxXy5S0TBo.srt @@ -0,0 +1,2371 @@ +1 +00:00:20,860 --> 00:00:25,360 +بسم الله الرحمن الرحيم استكملنا **موضوعًا** كتير مهم + +2 +00:00:25,360 --> 00:00:29,480 +بدأنا فيه اللي هو موضوع المضادات الحيوية، حدثنا + +3 +00:00:29,480 --> 00:00:34,600 +عن تعريفات، حدثنا عن تصنيفات، حدثنا عن mechanisms of + +4 +00:00:34,600 --> 00:00:37,540 +action، وبعدين انتقلنا لل resistance، وبعدين + +5 +00:00:37,540 --> 00:00:41,840 +انتقلنا ل how to test these antimicrobials في + +6 +00:00:41,840 --> 00:00:44,260 +المعمل، واخذنا ال disk diffusion وال broad + +7 +00:00:44,260 --> 00:00:50,010 +solution، وعرفنا مصطلحات زي ال MIC وال MBC، ووصلنا + +8 +00:00:50,010 --> 00:00:56,170 +للبرتقالة هذه اللي قدامنا اللي فيها action و + +9 +00:00:56,170 --> 00:00:59,170 +reaction، واحد بتفعل للأمام وواحد بتفعل للخلف، و + +10 +00:00:59,170 --> 00:01:04,050 +مكانك سامة، وهذه تعبير عن علاقة ما بين two + +11 +00:01:04,050 --> 00:01:11,580 +antibiotics، واحد بيثبت عمل الآخر، واحنا ممكن درسنا + +12 +00:01:11,580 --> 00:01:17,020 +قبل ذلك حاجة اسمها تآزر، أن واحد بيساعد عمل الآخر، + +13 +00:01:17,020 --> 00:01:20,140 +هذا الكلام درسناه لما كلمنا عن ال camp test في ال + +14 +00:01:20,140 --> 00:01:23,580 +group B streptococci لـ streptococcus agalactiae، + +15 +00:01:23,580 --> 00:01:27,460 +بأن واحد هيموليسين بيعمل تآذر، synergism مع + +16 +00:01:27,460 --> 00:01:31,300 +الهيموليسين تبع الـ staphylococcus aureus، فبزيد كمية + +17 +00:01:31,300 --> 00:01:33,180 +الهيموليسيس ويعطينا ال arrowhead shape إذا + +18 +00:01:33,180 --> 00:01:36,610 +باكرين، ال arrowhead shape، يبقى العلاقات اللي + +19 +00:01:36,610 --> 00:01:39,510 +بنتكلم عنها اليوم أربع علاقات، هو يا صبورة، وأطول + +20 +00:01:39,510 --> 00:01:44,090 +تلاتة، بنتكلم عن أربع علاقات، العلاقة الأولى + +21 +00:01:44,090 --> 00:01:47,330 +indifference، indifference يعني مش فارقة، يجب أن + +22 +00:01:47,330 --> 00:01:53,390 +نطلع اللوحة طول الوقت ونشوف مستوى البكتيريا جاهز + +23 +00:01:53,390 --> 00:01:58,110 +بيطلع لما يكون فيه no drugs، صح؟ بيكبر جاهز، لو حطينا + +24 +00:01:58,110 --> 00:02:02,430 +drug A، مستوى البكتيريا بيكون لعندها أجل، لكن لسه + +25 +00:02:02,430 --> 00:02:06,970 +فيه نمو، لو حطينا drug B، شوفوا كتير بنزل.. كتير + +26 +00:02:06,970 --> 00:02:11,190 +بنزل.. لكن لو حطينا الاثنين مع بعض.. لحظة أنه + +27 +00:02:11,190 --> 00:02:15,690 +مافيش اختلاف بين drug B لحاله وبين A وB، فبالتالي + +28 +00:02:15,690 --> 00:02:20,830 +A لم يضف لـ B، فبالتالي النتيجة indifference.. + +29 +00:02:20,830 --> 00:02:25,430 +مافيش فرق.. مافيش فرق، الشيء الثاني.. اتطلع هذا + +30 +00:02:25,430 --> 00:02:31,680 +.. هو drug، اتطلعوا الـ drug C، جريد من، يعني أن ال + +31 +00:02:31,680 --> 00:02:37,040 +activity لها ضعيفة جدا خالص، الـ drug D قطر البكتيريا + +32 +00:02:37,040 --> 00:02:41,860 +لكن من مستوى معين، لكن لما حطينا الـ drug C مع دي + +33 +00:02:41,860 --> 00:02:46,040 +شوفوا كيف التأثير رائع جدا، أكبر ما يكون، وبالتالي + +34 +00:02:46,040 --> 00:02:49,820 +يجب أن نقول أن 1 زائد 1 ساوي 5، 10، مش 1 زائد 1 + +35 +00:02:49,820 --> 00:02:53,100 +ساوي 2، في ده الحالة، في ال antagonism اللي هي + +36 +00:02:53,100 --> 00:03:02,180 +التالتة، drug، no drug E، لحظة، وdrug F، هي drug E وهي drug + +37 +00:03:02,180 --> 00:03:07,820 +F، drug F ممتاز كده، خفض، لما حطينا معاه E، قلل من ال + +38 +00:03:07,820 --> 00:03:11,380 +effect هو، يبقى هذا بالفعل زي البرتقال اللي شفناها + +39 +00:03:11,380 --> 00:03:15,840 +واحد بيشتغل وواحد بيخرّب الشغل، فهو اللي بيسميه + +40 +00:03:15,840 --> 00:03:17,800 +احنا antagonism + +41 +00:03:21,840 --> 00:03:25,120 +الرابع اللي بيقول عنها addition، واحد زائد + +42 +00:03:25,120 --> 00:03:29,560 +واحد يساوي 2، واحد بقى يقتل 10، و2 بيقتل + +43 +00:03:29,560 --> 00:03:33,440 +10، الآن لما أضفناهم مع بعض يقتلوا 20، هذا + +44 +00:03:33,440 --> 00:03:37,100 +simple mathematics، مثلًا مي addition، طيب اللي مش + +45 +00:03:37,100 --> 00:03:41,440 +مضكوكه على اللوحة وفي المحاضرة بدنا نكتبه كتاب إن + +46 +00:03:41,440 --> 00:03:45,380 +شاء الله، اكتبوا الآن Seidel زائد Seidel + +47 +00:03:48,940 --> 00:03:53,520 +حطه C زائد C، يعني جملة + +48 +00:03:53,520 --> 00:03:59,140 +جديدة، static زائد static، التالتة + +49 +00:03:59,140 --> 00:04:03,860 +الجملة التالتة اللي هي sidal زائد static، الأولى + +50 +00:04:03,860 --> 00:04:08,820 +بلد for sure، يعني قاعدة، الأولى اللي بعطينا + +51 +00:04:08,820 --> 00:04:13,420 +synergism، التانية + +52 +00:04:13,420 --> 00:04:19,020 +اللي هي static مع static بتعطينا Addition أو + +53 +00:04:19,020 --> 00:04:25,840 +Indifference، Addition أو Indifference، التالتة + +54 +00:04:25,840 --> 00:04:30,840 +Antagonism، لأن أقول لكم كيف يصير Antagonism بالعربي + +55 +00:04:30,840 --> 00:04:35,180 +البسيط، بالعربي البسيط، فاكرين الـ Antibiotic كيف + +56 +00:04:35,180 --> 00:04:40,720 +تشتغل؟ الـ antibiotic بتشتغل على الـ active bacteria، لما + +57 +00:04:40,720 --> 00:04:45,120 +شفنا الـ cell wall، كنا الـ cross link يمنع، مش + +58 +00:04:45,120 --> 00:04:50,360 +بيتجسجس، بيمنع، طب هو لما يكون already موجود يبقى + +59 +00:04:50,360 --> 00:04:55,220 +ما فيش إمكانية لـ antibiotic أن تعمل أي حاجة + +60 +00:04:55,220 --> 00:05:00,110 +وبالتالي فقط bacteria actively growing are affected + +61 +00:05:00,110 --> 00:05:03,770 +طب البكتيريا static هيوقف نشاط البكتيريا، يبقى + +62 +00:05:03,770 --> 00:05:06,430 +البكتيريا صاحبة اللي قبل يكون معاه مش هيقتل + +63 +00:05:06,430 --> 00:05:08,710 +البكتيريا، وهذا اللي بيصير فيه أننا كنا فيه + +64 +00:05:08,710 --> 00:05:12,910 +antagonism، هذا الكلام مهم جدا ليش؟ لأنه + +65 +00:05:12,910 --> 00:05:18,610 +practically ممكن الطبيب يوصف للمريض two drugs إذا + +66 +00:05:18,610 --> 00:05:21,870 +واحد منهم static وواحد منهم cidal، هيصير أن ال + +67 +00:05:21,870 --> 00:05:25,150 +energy، الأهم اللي هو ال antagonism، وبالتالي بدل + +68 +00:05:25,150 --> 00:05:27,770 +ما نزيد التأثير، وأنتم عارفين بنعطي two + +69 +00:05:27,770 --> 00:05:32,110 +antibiotics حتى نحسن من الـ.. الـ.. القدرة + +70 +00:05:32,110 --> 00:05:35,250 +العلاجية للـ antibiotic ونقصر الفترة والأخرى، لكن + +71 +00:05:35,250 --> 00:05:38,070 +في هذه الحالة احنا بنكون يعني أسأنا الاختيار + +72 +00:05:40,400 --> 00:05:45,880 +الصور اللي قدامنا مش كتير واضحة، لكن بدنا نحاول أن + +73 +00:05:45,880 --> 00:05:49,840 +نطلع منها.. لأ كويسة، ميّحة، شايفين الدائرة اللي.. + +74 +00:05:49,840 --> 00:05:53,640 +اللي.. اللي بالخط المجزق وراها على طول هنا فيه + +75 +00:05:53,640 --> 00:06:00,400 +bacterial growth، صح؟ لكن استكمال الدائرة المفروض + +76 +00:06:00,400 --> 00:06:03,280 +أن أنتم عارفين أن الظنب النامشي ليش بتكون دائرية + +77 +00:06:03,280 --> 00:06:07,760 +لأن القرص دائري وال diffusion دائري، ليش في المنطقة + +78 +00:06:07,760 --> 00:06:14,230 +هذه اللي هي بعد قطر الدائرة مشي بكتيريا، ال + +79 +00:06:14,230 --> 00:06:17,970 +antibiotic التاني، ما هو هي دائرته كل واحد هيحدود + +80 +00:06:17,970 --> 00:06:23,510 +المفروض هيحدود الافتراضية لإن التقاء X مع Y عمل + +81 +00:06:23,510 --> 00:06:26,550 +سيناريو انفراد من قدرة القتل في المنطقة اللي بين + +82 +00:06:26,550 --> 00:06:31,370 +التاني، لأنه خذ بالكم هذه هي منطقة التقاء الـ two + +83 +00:06:31,370 --> 00:06:35,290 +antibiotics، هنا antibiotic الأولاني مش هيكون موجود + +84 +00:06:35,290 --> 00:06:41,940 +صح؟ وفي المنطقة الثانية فقط في منطقتها المنتصف، إذا + +85 +00:06:41,940 --> 00:06:45,120 +ممكن احنا نشوف ال scenario زي اللي ممكن نعمل + +86 +00:06:45,120 --> 00:06:48,440 +sensitivity testing بالـ disk diffusion + +87 +00:06:51,360 --> 00:06:54,000 +طيب، the future of chemotherapeutic areas، احنا + +88 +00:06:54,000 --> 00:06:58,000 +عملنا صورة سوداء قاتمة على موضوع الـ resistance و + +89 +00:06:58,000 --> 00:07:01,760 +أنه ولسه من بارحة كنا بنكتب في تقرير scientific + +90 +00:07:01,760 --> 00:07:06,020 +من جزء من الشغل اللي بنشتغله في البحر، تقرير قاتم + +91 +00:07:06,020 --> 00:07:10,040 +الصحي، يعني ال percentage of resistance للـ + +92 +00:07:10,040 --> 00:07:13,380 +enterococcus للفانكومايسين في البيئة عمالة ترتفع + +93 +00:07:13,380 --> 00:07:19,520 +بشكل مخيف، طب هل هناك من مستقبل مشرق في هذا المجال؟ + +94 +00:07:19,520 --> 00:07:23,060 +هل هناك أمل يعني الباحثين بيشتغلوا عليه مثلًا؟ + +95 +00:07:23,060 --> 00:07:27,760 +الواقع أن كلمة مستقبل، مستقبل في زماننا اللي احنا + +96 +00:07:27,760 --> 00:07:33,480 +فيه هذه كلمة فاضفاضة ولا تعني يعني بدقة أن احنا + +97 +00:07:33,480 --> 00:07:36,300 +بنتكلم عن مستقبل قريب ولا بعيد، لأن الأحداث + +98 +00:07:36,300 --> 00:07:41,580 +المتلاحقة، التطورات مش بطيئة زي القرون الماضية + +99 +00:07:41,580 --> 00:07:44,340 +تطورت الإنسانية جدا، من كان يتخيل إن الراديو اللي + +100 +00:07:44,340 --> 00:07:47,260 +كان حجمه الجد يصير تطبيق application في ال mobile + +101 +00:07:47,260 --> 00:07:51,580 +تبعنا، تمام؟ وليس يعني جهاز ضخم، التلفزيون كذلك + +102 +00:07:51,580 --> 00:07:55,040 +الأمر، الحواسيب وسرعتها، الموبايلات نفسها كيف + +103 +00:07:55,040 --> 00:08:00,030 +تطورت؟ فما نقول إن ال future قد يكون already + +104 +00:08:00,030 --> 00:08:03,950 +passed، يعني صار passed في بعض اللحظات، لكن دائما + +105 +00:08:03,950 --> 00:08:08,570 +هناك frontiers، يعني مجالات لم يطرقها الإنسان أو + +106 +00:08:08,570 --> 00:08:12,890 +طرقها ورد فيها باساس أمل، فبيشجع الناس دائما أنه + +107 +00:08:12,890 --> 00:08:16,000 +يعني يطرقوا ويُبحثوا في هذه المجالات، من هذه + +108 +00:08:16,000 --> 00:08:20,180 +المجالات، مجالات درسنا احنا حزمة بيبتايد انتيبايوتكس + +109 +00:08:20,180 --> 00:08:23,140 +بيبتايد انتيبايوتكس، اخذنا واحد اسمه بيبتايد بيبتايد + +110 +00:08:23,140 --> 00:08:25,980 +بيبتايد بيبتايد بيبتايد، واخذنا واحد اسمه جلايكو + +111 +00:08:25,980 --> 00:08:26,960 +بيبتايد، جلايكو بيبتايد، جلايكو بيبتايد، جلايكو + +112 +00:08:26,960 --> 00:08:28,000 +بيبتايد، جلايكو بيبتايد، جلايكو بيبتايد، جلايكو + +113 +00:08:28,000 --> 00:08:28,380 +بيبتايد، جلايكو بيبتايد، جلايكو بيبتايد، جلايكو + +114 +00:08:28,380 --> 00:08:32,860 +بيبتايد، جلايكو بيبتايد، جلايكو + +115 +00:08:32,860 --> 00:08:35,580 +بيب + +116 +00:08:36,040 --> 00:08:41,060 +فكويس إنه ندور على peptide احنا نصنعها، يعني نحاول + +117 +00:08:41,060 --> 00:08:43,840 +نصنع peptides جديدة بالهندسة الوراثية، اللي هو سهل + +118 +00:08:43,840 --> 00:08:48,220 +أنه نعمل ترتيبة لـ peptides عن طريق أنه نصنع DNA وبعدين + +119 +00:08:48,220 --> 00:08:53,420 +بعدين الـ RNA، دي كمان شغل ثاني، الحيوانات اللي + +120 +00:08:53,420 --> 00:09:00,090 +عايشة وبتحتك، يعني بيئات احنا.. احنا يعني الإنسان + +121 +00:09:00,090 --> 00:09:04,630 +مستوى النظافة عنده عالي جدا، لكن في بيئات الحيوانات + +122 +00:09:04,630 --> 00:09:08,470 +زي أكلمة مضال خنازير مثلًا على سبيل المثال أو + +123 +00:09:08,470 --> 00:09:12,330 +الأسماك، زي سمكة القرش في البحر بتتناول GF و + +124 +00:09:12,330 --> 00:09:15,470 +بتتناول فمليانة بتكون بكتيريا، فغالبا بيكون في + +125 +00:09:15,470 --> 00:09:21,460 +أجسامها مركبات لمكافحة، فمثلًا السكورامين أخذوه ك + +126 +00:09:21,460 --> 00:09:25,680 +antibiotic من أجسام أسماك القرش، هو الـ sharks، فيه + +127 +00:09:25,680 --> 00:09:29,240 +الـ protagreen من الـ pigs، اللي هو الخنازير + +128 +00:09:29,240 --> 00:09:31,900 +أكرا ومقوم الله، والحلس اللي ما عندك بتحكوله، + +129 +00:09:31,900 --> 00:09:38,930 +المجانين من الـ frogs، تمام؟ الآن طبعا هذه أمثلة و + +130 +00:09:38,930 --> 00:09:42,930 +ليست قائمة حصرية يعني حتى البندورة/الطماطم تمام + +131 +00:09:42,930 --> 00:09:46,950 +فيها تماتين مركبات برضه إيه بتاعة مقلة لكن إذا + +132 +00:09:46,950 --> 00:09:51,590 +بتلاحظوا الـ tomato برضه بتعفن المعنى الآخر إنه مش + +133 +00:09:51,590 --> 00:09:56,270 +لكل شيء مش لكل شيء أو الكميات بتكون قليلة فاحنا + +134 +00:09:56,270 --> 00:10:01,030 +ندرس إمكانية تحوير هذه المركبات أو إمكانية الـ + +135 +00:10:01,030 --> 00:10:03,990 +synthesis .. الـ synthesis إلها بمعنى إنه ننتجها + +136 +00:10:03,990 --> 00:10:08,500 +بكميات كبيرة، الأخيرة حلوة تقنية كويسة اسمها + +137 +00:10:08,500 --> 00:10:12,600 +antisense technology أو antisense agents ورقصه + +138 +00:10:12,600 --> 00:10:17,720 +معايا في الهندسة دائما احنا الـ protein هو اللغة + +139 +00:10:17,720 --> 00:10:21,080 +اللي بتكلم فيها الخلية بسميها الـ phenotype و + +140 +00:10:21,080 --> 00:10:25,620 +المادة الوراثية الـ genotype يعبر الـ gene عن نفسه + +141 +00:10:25,620 --> 00:10:30,740 +بإنتاج الـ protein بسميها gene expression عبر الـ + +142 +00:10:30,740 --> 00:10:34,600 +gene expression في مرحلة بسيطة بتحول الـ DNA إلى أو + +143 +00:10:34,600 --> 00:10:39,580 +بيصيله transcription بتطلع منه mRNA الـ mRNA لو أنا + +144 +00:10:39,580 --> 00:10:45,820 +جيبت قطعة to complement the RNA إيش هيصير + +145 +00:10:45,820 --> 00:10:51,000 +معاه؟ هيصير double-stranded RNA حد مش .. مصطلح مش + +146 +00:10:51,000 --> 00:10:55,580 +دارج هنا double-stranded RNA مش مصطلح دارج هنا هو + +147 +00:10:55,580 --> 00:10:59,870 +عبارة عن single strand هذا الـ .. الـ .. الـ + +148 +00:10:59,870 --> 00:11:04,010 +complementary strand احنا بنسميه antisense لإن في + +149 +00:11:04,010 --> 00:11:07,710 +مصطلح كمان إنه الـ .. الـ mRNA بيسموه positive sense + +150 +00:11:07,710 --> 00:11:10,790 +positive sense يعني جاهز للـ .. للـ .. للـ .. للـ + +151 +00:11:10,790 --> 00:11:14,090 +translation لإن هت .. في نفس المحاضرة اليوم هتكلم + +152 +00:11:14,090 --> 00:11:16,750 +عن الفيروسات هتكلم عن حالة اسمها الـ negative sense + +153 +00:11:16,750 --> 00:11:23,230 +مادة وراثية .. مادة وراثية RNA لكن مش جاهزة إنها + +154 +00:11:23,230 --> 00:11:28,230 +يصبح translation لازم تتحول لـ positive sense عموما + +155 +00:11:28,230 --> 00:11:32,830 +الآن صار في عندي complementary strand للـ mRNA بطل + +156 +00:11:32,830 --> 00:11:37,210 +الريبوزوم قدر إنه يعمله expression يعني translation + +157 +00:11:37,210 --> 00:11:42,130 +وبالتالي أنا ممكن أواجه تصنية vital protein في + +158 +00:11:42,130 --> 00:11:46,220 +الخلية البكتيرية وبالتالي أموت البكتيريا هذا tech + +159 +00:11:46,220 --> 00:11:49,860 +تقنية شغالة إلها كويس وأنتجوا بعض المنتجات لكن + +160 +00:11:49,860 --> 00:11:53,560 +بتظهر عملية إنه يعني تخيل ماناخدها oral كيف بيبقى + +161 +00:11:53,560 --> 00:11:56,840 +متص الـ .. الـ antisense كيف بيدخل الخلايا هذا موضوع + +162 +00:11:56,840 --> 00:12:00,560 +شوية معظم البيانات اللي بنكون بتاعتنا من الجزء + +163 +00:12:00,560 --> 00:12:03,340 +الأول الصحيح من المحادثة اليوم اللي هو الـ + +164 +00:12:03,340 --> 00:12:07,760 +antimicrobials هنزلكوا بعض الـ animation إضافية + +165 +00:12:07,760 --> 00:12:11,020 +كمان إن شاء الله متعلقة بهذا الموضوع + +166 +00:12:18,040 --> 00:12:23,040 +الموضوع الجديد اليوم اللي هو الفيروس هذا طبعا + +167 +00:12:23,040 --> 00:12:27,300 +أنتوا عارفين إنه بيكون كورس كامل اسمه Virology لكن + +168 +00:12:27,300 --> 00:12:31,800 +في essential medical microbiology لازم نغطي بعض + +169 +00:12:31,800 --> 00:12:34,860 +الجوانب المتعلقة بالفيروس وعلى الأقل هتكلم عن + +170 +00:12:34,860 --> 00:12:38,560 +شغلتين على الـ viral structure وعلى الـ viral + +171 +00:12:38,560 --> 00:12:42,020 +replication خلال الأسبوع هذا اللي هو اليوم نهايته + +172 +00:12:42,020 --> 00:12:44,420 +والأسبوع القادم اللي بنني فيه إن شاء الله الـ + +173 +00:12:44,420 --> 00:12:44,660 +course + +174 +00:12:50,510 --> 00:12:54,430 +ما يميز الفيروسات إنه كان فيها ليها خلاف في يوم من + +175 +00:12:54,430 --> 00:12:57,310 +الأيام، هل هي كائنات حية أو أعتقد أنهم نقلوا + +176 +00:12:57,310 --> 00:13:00,750 +الكورة في المدارس وأنا بزمي جدل عقيم، يعني الأصل + +177 +00:13:00,750 --> 00:13:04,410 +ما نجادل فيه، يعني مش دائما التصنيفات .. مش دائما + +178 +00:13:04,410 --> 00:13:07,910 +التصنيفات إنه كائن حي ولا جماد يعني مفيدة لنا، هو + +179 +00:13:07,910 --> 00:13:10,530 +يمتلك صفة من صفات الحياة اللي هي الـ replication + +180 +00:13:10,530 --> 00:13:14,410 +فقط في حال وجوده داخل الخلايا، وتانية من الجثة، + +181 +00:13:14,410 --> 00:13:19,490 +هي معلومة بتميز هذه الكائنات اللي اسمها فيروس لكن + +182 +00:13:19,490 --> 00:13:23,450 +وصفناها إنها obligate intracellular parasites لهذا + +183 +00:13:23,450 --> 00:13:29,430 +السبب إنه لا تستطيع التكاثر خارج الخلية، السؤال لماذا + +184 +00:13:29,430 --> 00:13:34,230 +لا تستطيع التكاثر خارج الخلية؟ لا .. لا .. لا تمتلك + +185 +00:13:34,230 --> 00:13:38,050 +مقوم .. لا .. لا .. لها DNA و .. لها DNA ورنية لكن + +186 +00:13:38,050 --> 00:13:42,430 +لا تمتلك الـ ribosomes لا تمتلك بظبط اللي هي الـ + +187 +00:13:42,430 --> 00:13:46,690 +organelles ما بتنتلكش الـ machinery اللي هي المعدات + +188 +00:13:46,690 --> 00:13:51,670 +اللي موجودة في الخلية العادية وبالتالي تضطر آسفة + +189 +00:13:51,670 --> 00:13:57,550 +إنها تتطفل على عوائل أخرى للحصول على هذه الأدوات + +190 +00:13:57,550 --> 00:14:02,960 +والمعدات اللي احنا حكينا عنها because they can + +191 +00:14:02,960 --> 00:14:06,260 +reproduce only within cells, viruses are obligate + +192 +00:14:06,260 --> 00:14:09,840 +intracellular parasites هذه الصفة لم نرها في أي + +193 +00:14:09,840 --> 00:14:13,020 +بكتيريا إلا في دنتين كلاميديا وريكتسيا ممتاز + +194 +00:14:13,020 --> 00:14:16,620 +كلاميديا وريكتسيا and both of them they cannot + +195 +00:14:16,620 --> 00:14:20,080 +replicate independently خدوا بالكم فرق كبير بين + +196 +00:14:20,080 --> 00:14:23,500 +الكلاميديا وريكتسيا وما بين الفيروس الكلاميديا + +197 +00:14:23,500 --> 00:14:29,020 +وريكتسيا are بكتيريا كل المكونات موجودة ما عدا + +198 +00:14:29,020 --> 00:14:35,730 +عندها مشكلة واحدة they cannot synthesize ATP يعني + +199 +00:14:35,730 --> 00:14:40,630 +ماهموش فلوس يشتروا أغراض من في الخلية فبالتالي أو + +200 +00:14:40,630 --> 00:14:44,790 +يتبادلوا الطاقة بالخلية أنتم عارفين التبادل بيكون + +201 +00:14:44,790 --> 00:14:49,570 +بالـ ATP فهم بيسموهم حتى إنهم اسم لطيف بيسموهم + +202 +00:14:49,570 --> 00:14:53,690 +energy parasites يعني بتتففر من أجل الـ energy اللي + +203 +00:14:53,690 --> 00:14:58,070 +هو الـ ATP viruses + +204 +00:14:58,070 --> 00:15:03,000 +do not replicate in a similar way to that of cells + +205 +00:15:03,000 --> 00:15:07,520 +الخلايا سواء كانت Eukaryote أو بلوكاريوت فيها إنه + +206 +00:15:07,520 --> 00:15:10,140 +simple binary vision انقسام ثنائي وآخر الخلية + +207 +00:15:10,140 --> 00:15:14,020 +تصير تنتهي وفي الـ mitosis وفي الـ meiosis برضه كمان + +208 +00:15:14,020 --> 00:15:17,620 +نفس الجثة فيها اندي انقسام واضح إنه فيه زيادة في + +209 +00:15:17,620 --> 00:15:21,720 +عدد الخلايا الواحدة بصير تنتهي لكن الفيروسات الـ + +210 +00:15:21,720 --> 00:15:25,440 +virus will enter the whole cell one single + +211 +00:15:25,440 --> 00:15:29,180 +particle ويت will be released hundreds and + +212 +00:15:29,180 --> 00:15:32,260 +thousands على حسب نوع الفيروس نفسه hundreds and + +213 +00:15:32,260 --> 00:15:34,560 +thousands of particles are released from this + +214 +00:15:34,560 --> 00:15:38,300 +cell، إذا أنت قامي تكاثرها واتبعته مختلفة تمام + +215 +00:15:41,450 --> 00:15:46,210 +general characteristics للـ viruses الـ viruses أول + +216 +00:15:46,210 --> 00:15:51,290 +هام فيها إنه يا المادة الوراثية يا إما DNA يا إما + +217 +00:15:51,290 --> 00:15:57,350 +RNA but never both اللي أعقد من هيك نوع المادة + +218 +00:15:57,350 --> 00:15:59,070 +الوراثية مش هتكلم فيها اللي احتجت لانا في لوحات + +219 +00:15:59,070 --> 00:16:02,950 +سابقة المادة البراطينية نفسها احنا كلنا نعرف DNA + +220 +00:16:02,950 --> 00:16:07,150 +double-strand والـ RNA single-strand وبالتحديد هنا + +221 +00:16:07,150 --> 00:16:10,410 +هنشوف إنه فينا double-stranded DNA وsingle + +222 +00:16:10,410 --> 00:16:13,550 +-stranded RNA و double-stranded mRNA و single + +223 +00:16:13,550 --> 00:16:17,730 +-stranded mRNA وهنشوف في الـ single-stranded + +224 +00:16:17,730 --> 00:16:23,050 +positive sense و negative sense وهنشوف لينيا أو + +225 +00:16:23,050 --> 00:16:27,930 +segmented مقطع يعني بدل من جنوب يكون قطع واحدة + +226 +00:16:27,930 --> 00:16:35,740 +يكون قدت قطعبعض الفيروسات تمتلك ممبرين حول الكبسيط + +227 +00:16:35,740 --> 00:16:39,980 +تبعتها والبعض الآخر لايمتلكها فبنقول عنها envelope + +228 +00:16:39,980 --> 00:16:44,200 +و non envelope اللي إلها بسميها envelope عشان نكون + +229 +00:16:44,200 --> 00:16:46,720 +واضحينه من البداية هذا الـ envelope من أين بيجي؟ + +230 +00:16:47,120 --> 00:16:51,900 +بييجي الفيروس هو الطالع يا إما من الـ nuclear + +231 +00:16:51,900 --> 00:16:56,720 +membrane يا من الـ cell membrane يبقى مصدر .. مصدر + +232 +00:16:56,720 --> 00:17:00,800 +الـ envelope هو من الـ nuclear membrane أو من الـ + +233 +00:17:00,800 --> 00:17:05,760 +cell membrane تبع الـ host cell مين أجواء؟ مين + +234 +00:17:05,760 --> 00:17:09,220 +أحسن؟ اللي له envelope ولا اللي ماروش envelope؟ + +235 +00:17:09,220 --> 00:17:17,240 +حسب .. حسب .. حسب إجابة .. حسب ما يتبعها تبرير طالع + +236 +00:17:17,240 --> 00:17:24,040 +عليك اللي أخد membrane استفاد حاجة إيش الحاجة هذه؟ + +237 +00:17:24,040 --> 00:17:28,640 +إنه الآن لو دخل لـ host تاني الـ membrane اللي على + +238 +00:17:28,640 --> 00:17:33,940 +جدفه اللي حامله تمام الـ envelope هو جزء من الـ .. + +239 +00:17:33,940 --> 00:17:37,380 +الـ .. الـ host وبالتالي سهل جدا إنه membrane مع + +240 +00:17:37,380 --> 00:17:41,160 +membrane zero مع fusion اه fusion فبدخل الفيروس + +241 +00:17:41,160 --> 00:17:47,150 +داخل خلية بكل سهولة يجي هي الميزة إنه مابيعانيش من + +242 +00:17:47,150 --> 00:17:51,370 +موضوع الـ penetration والـ attachment لكن خدوا + +243 +00:17:51,370 --> 00:17:57,230 +بالكم مدام اركن واعتمد على الـ envelope يدخله + +244 +00:17:57,230 --> 00:18:01,070 +الخلايا لو راح الـ envelope هذا معناه الحكي إنه مش + +245 +00:18:01,070 --> 00:18:07,650 +هاجدر يعمل infection وما أسهل أن تذهب أو يذهب هذا + +246 +00:18:07,650 --> 00:18:12,510 +الـ envelope ليش اتذكروا membrane فاكرين الـ olive + +247 +00:18:12,510 --> 00:18:16,750 +oil؟ اللي وزي الزيتون، very fragile، لو غسلنا + +248 +00:18:16,750 --> 00:18:19,430 +إيدينا بصبون، على طول الصبون بيعمل إيه؟ + +249 +00:18:19,430 --> 00:18:23,590 +Destruction للـ ممبرين، لو جاف الـ ممبرين، الفيروس + +250 +00:18:23,590 --> 00:18:27,790 +يعني جافي، الممبرين راحوا، لو بلعناها في الأمعاء، + +251 +00:18:27,790 --> 00:18:32,690 +في الـ .. في الـ .. في الصمت، هيتدمرب الجي معنى الحكي + +252 +00:18:32,690 --> 00:18:37,650 +أنه بيعطيه نوع من الحساسية وعدم المقاومة عدم + +253 +00:18:37,650 --> 00:18:41,870 +المقاومة في الـ environment لذلك لا نجد ولا + +254 +00:18:41,870 --> 00:18:44,870 +envelope virus بيعمل infection في الـ + +255 +00:18:44,870 --> 00:18:48,290 +gastrointestinal tract كلهم بيعمله في الغالب + +256 +00:18:48,290 --> 00:18:51,050 +respiratory tract infection respiratory tract + +257 +00:18:51,050 --> 00:18:55,910 +infection وهشوف طبعًا أمثلة عليهم لاحقًا لكن اللي + +258 +00:18:55,910 --> 00:18:58,950 +بدنا نفهمه أنه مش وجود .. موجود envelope يعني أنه + +259 +00:18:58,950 --> 00:19:04,750 +حاجة يعني .. مية بالمية بتعطي الفيروس حاجة قوة أو + +260 +00:19:04,750 --> 00:19:07,330 +صلابة في البيئة لأ بالعكس بتعطيه عدم الـ stability + +261 +00:19:07,330 --> 00:19:14,650 +في الـ environment طيب الفيروسات بتحتوي على مادة + +262 +00:19:14,650 --> 00:19:18,170 +وراثية وأن هي DNA أما RNA لكن واقع الأمر لا + +263 +00:19:18,170 --> 00:19:24,670 +تمتلك nucleus لا تمتلك nucleus بينما الـ eucariotes + +264 +00:19:24,670 --> 00:19:28,390 +كلها تمتلك nucleus طب والـ eucariotes؟ + +265 +00:19:33,920 --> 00:19:37,840 +الفروقات الأساسية واللي بيميز الـ eukaryotes هو + +266 +00:19:37,840 --> 00:19:41,800 +عشان احنا نكون واضحين هو فيه nucleus بس مش الـ true + +267 +00:19:41,800 --> 00:19:45,920 +nucleus سميناها لأن مافيش فيها nuclear membrane + +268 +00:19:45,920 --> 00:19:51,440 +فسميناها .. يعني مش الـ true nucleus تمام؟ بينما الـ + +269 +00:19:51,440 --> 00:19:56,260 +eukaryotes لأن فيها سميناها true nucleus لكن فيها + +270 +00:19:56,260 --> 00:20:00,300 +مادة وراثية، فيها كروموزوم صح، البكتيريا، فيها كل + +271 +00:20:00,300 --> 00:20:04,360 +الـ machinery، لكن مش فيها mitochondria، لكن الـ .. + +272 +00:20:04,360 --> 00:20:07,060 +يعني بِمَا أخر في فروقات بين الفيروسات وبين + +273 +00:20:07,060 --> 00:20:11,260 +البروكاريوتس وبين الـ .. لكن في النهاية + +274 +00:20:11,260 --> 00:20:15,080 +البروكاريوتس أقرب إلى الـ eukaryotes من مين؟ من + +275 +00:20:15,080 --> 00:20:15,900 +الفيروسات + +276 +00:20:20,350 --> 00:20:25,630 +هذا بالطبع بيلخص الأمر تمامًا صحيح types of nucleic + +277 +00:20:25,630 --> 00:20:30,070 +acid الـ virus is DNA or RNA but never both لكن الـ + +278 +00:20:30,070 --> 00:20:33,930 +cells بتكون فيها كل من الـ DNA والـ RNA الـ proteins + +279 +00:20:33,930 --> 00:20:38,110 +بعض الـ فيروسات تحتوي + +280 +00:20:38,110 --> 00:20:42,790 +على protein منها matrix protein منها منها + +281 +00:20:42,790 --> 00:20:48,390 +glycoproteins منها ما هو enzymes تخيلوا فيروس عنده + +282 +00:20:48,390 --> 00:20:53,630 +الـ genome تبعه RNA و negative sense، بده يروح على + +283 +00:20:53,630 --> 00:20:57,570 +خلية، هو مفكر، كل ما يروح، بيقول طب أنا روح على + +284 +00:20:57,570 --> 00:21:01,560 +الخلية، بدأ أجي عندهم بيلاقي عندهم enzymes أستفيد + +285 +00:21:01,560 --> 00:21:04,600 +منها في إن أنا أتحول من negative sense لـ positive + +286 +00:21:04,600 --> 00:21:09,220 +sense الجواب لأ فبيقول طب أنا بتصنع الـ enzyme تبقى + +287 +00:21:09,220 --> 00:21:12,860 +هدوء أفضل معاه وأنا رايح على الخلية فبعض الـ .. الـ + +288 +00:21:12,860 --> 00:21:16,540 +.. الـ بيروزات بتحمل معاها كما ذكرنا بعض الـ enzyme + +289 +00:21:16,540 --> 00:21:21,360 +زي الـ HIV virus بيحمل معاه reverse transcriptase + +290 +00:21:21,360 --> 00:21:25,980 +reverse transcriptase بينما الخلايا فيها حياتها + +291 +00:21:25,980 --> 00:21:28,340 +كلها proteins حياتها كلها الـ proteins + +292 +00:21:34,490 --> 00:21:41,170 +الخلايا كلها نبات، حيوان، بكتيريا، فطريات في الـ H + +293 +00:21:41,170 --> 00:21:47,040 +cell Ribosomes absent لكن في إشارة إنه في معلومة + +294 +00:21:47,040 --> 00:21:50,980 +تحت بتقول إنه بعض الفيروسات اللي اسمها Arena + +295 +00:21:50,980 --> 00:21:55,840 +viruses تحتوي على non-functional ribosomes إيش هي + +296 +00:21:55,840 --> 00:22:00,200 +non-functional ribosomes؟ يعني ريبوزومات ملاش .. + +297 +00:22:00,200 --> 00:22:04,940 +لأ غير فعالة، غير نشطة، يعني حتى لو حطنا لها mRNA + +298 +00:22:04,940 --> 00:22:09,020 +مش هتشتغل، non-functional، غير عن كلمة مش معروفة + +299 +00:22:09,020 --> 00:22:09,900 +الوظيفة + +300 +00:22:12,570 --> 00:22:17,070 +لأ كلمة مالهاش وظيفة يعني إن هي شغالة بس مش كده + +301 +00:22:17,070 --> 00:22:20,230 +تعمل حاجة، بس non-functional يعني خربانة خدنا + +302 +00:22:20,230 --> 00:22:24,170 +نقول، يعني لو حطنا لها احنا إيه معلنة إيه وكده مش + +303 +00:22:24,170 --> 00:22:24,570 +هتشتغل + +304 +00:22:28,500 --> 00:22:31,980 +قلنا الـ .. الـ .. الـ .. الـ metachondria absent + +305 +00:22:31,980 --> 00:22:35,600 +بينما في الـ .. في الـ eukaryotes بتكون موجودة الـ + +306 +00:22:35,600 --> 00:22:39,760 +enzymes none or few يا إما مافيش يا إما قليلة + +307 +00:22:39,760 --> 00:22:43,520 +بينما الخلايا كتير الـ multiplication by binary + +308 +00:22:43,520 --> 00:22:47,920 +vision الخلايا نعم بينما اللي هو الـ viruses + +309 +00:22:47,920 --> 00:22:51,960 +مابتتنقسمش بالطريقة هذه واحنا قلنا إنه one cell + +310 +00:22:51,960 --> 00:22:54,740 +one virus one particle واللي مدى هو several + +311 +00:22:54,740 --> 00:23:00,010 +hundred particles هي جيه الـ size تبقى للميروسة Size + +312 +00:23:00,010 --> 00:23:05,210 +الحجم اللي احنا شفناه في البكتيريا كنا نقوله إنه + +313 +00:23:05,210 --> 00:23:09,710 +واحد مايكرون وشحته هذا الواحد مايكرون صار الألف + +314 +00:23:09,710 --> 00:23:15,590 +نانومتر الألف لحظة أكبر فيروس 300 نانومتر يعني + +315 +00:23:15,590 --> 00:23:19,250 +البكتيريا صغيرة واحد مايكرون حجمها ثلاث أضعاف حجم + +316 +00:23:19,250 --> 00:23:19,570 +اللي هو + +317 +00:23:23,170 --> 00:23:28,890 +والبكتيريا الصغيرة خالص قد تصل بعض البروتينات في + +318 +00:23:28,890 --> 00:23:34,590 +حجمها إلى 20 نانومتر وقد تصل بعض البكتيريا في + +319 +00:23:34,590 --> 00:23:39,410 +صغرها إلى 300 نانومتر تشوف الحجم هنا range واسع + +320 +00:23:39,410 --> 00:23:47,070 +أكبر بروتين 20 نانومتر ثلاث مئة نانومتر مين أصغر + +321 +00:23:47,070 --> 00:23:51,730 +البكتيريا؟ أظن الـ micro plasma دي كمان بكتيريا + +322 +00:23:51,730 --> 00:23:53,810 +اسمها كلاميديا بس كنا بنتكلم عنها قبل شوية + +323 +00:23:53,810 --> 00:23:58,190 +الكلاميديا في إليها طورين تختلف عن باقي أنواع + +324 +00:23:58,190 --> 00:24:01,470 +البكتيريا اللي في الدنيا بأن فيها stages مين درسه + +325 +00:24:01,470 --> 00:24:07,250 +فيلياد؟ طفلياد طب بتعرفوا حاجة اسمها الـ cyst الطور + +326 +00:24:07,250 --> 00:24:11,590 +المتحوصل و Trophocyte اللي هو الطور المتغذى الخضري + +327 +00:24:11,590 --> 00:24:16,980 +هذه مرحلتين الخلية ممكن تتحول من Etrophosite إلى + +328 +00:24:16,980 --> 00:24:22,720 +Cyst الـ Cyst أكتر مقاومة من مين؟ من Etrophosite طب + +329 +00:24:22,720 --> 00:24:25,380 +هذا الكلام في البكتيريا ما نشوفهوش مافيش life cycle + +330 +00:24:25,380 --> 00:24:29,220 +في البكتيريا أساسًا مافيش life cycle إلا في + +331 +00:24:29,220 --> 00:24:34,940 +البكتيريا هذه فيها life cycle تتحول من reticulate + +332 +00:24:34,940 --> 00:24:40,520 +body إلى ما يشبه الـ sports إلى ما يشبه الـ sport + +333 +00:24:40,520 --> 00:24:44,100 +هنشوفها كمان شوية ففيها نوع من الـ life cycle خدنا + +334 +00:24:44,100 --> 00:24:48,260 +نقول وين كانت الـ life cycle بسيطة إذا الـ size + +335 +00:24:48,260 --> 00:24:52,180 +بتروح من 20 نانومتر إلى 300 نانومتر وهذا الكلام لن + +336 +00:24:52,180 --> 00:24:55,480 +نستطيع بأي حالة من الأحوال رؤيته بوضوح تحت الـ + +337 +00:24:55,480 --> 00:24:59,680 +microscope العام إذا البكتيريا الرواية اللي طولها 2 + +338 +00:24:59,680 --> 00:25:04,000 +مايكرون و3 مايكرون على قوة تكبير ألف بنشوفها شحطة + +339 +00:25:04,000 --> 00:25:07,040 +حمرة صغيرة بدون أي تفاصيل من هناك نشوف الفلاجلة + +340 +00:25:07,040 --> 00:25:13,020 +تبعها فبكل بيروسات بدنا electron microscope their + +341 +00:25:13,020 --> 00:25:16,580 +shapes are frequently referred to in colloquial + +342 +00:25:16,580 --> 00:25:22,990 +terms يعني مصطلحات تشبيهية خلينا نسميها مثلًا بنقول + +343 +00:25:22,990 --> 00:25:27,250 +شكله زي الكورة، شكله زي القضيب يعني أو العصي أو الـ + +344 +00:25:27,250 --> 00:25:31,150 +.. الـ .. الـ .. البسلاي أو الـ bullet، رصاصة مثلًا + +345 +00:25:31,150 --> 00:25:35,830 +تمام؟ يعني بيعطيهم أوصاف للشكل الخارجي كما تبدو + +346 +00:25:35,830 --> 00:25:38,650 +تحت الـ electron microscope زي الأخيرة الـ bricks + +347 +00:25:38,650 --> 00:25:44,470 +اللي هو الطوب، الـ block يعني but in reality احنا + +348 +00:25:44,470 --> 00:25:49,070 +بيعطيها للأوصاف بناء على إيش قلنا؟ على شيء خارجي لكن + +349 +00:25:49,070 --> 00:25:54,170 +in reality they are complex in structures and they + +350 +00:25:54,170 --> 00:25:58,190 +usually have symmetry، إيش هي symmetry؟ تماثل، هذا + +351 +00:25:58,190 --> 00:26:04,710 +تماثل دقيق، هندسي دقيق، geometric symmetry وكلمة + +352 +00:26:04,710 --> 00:26:11,790 +precise معناها تماثل هندسي دقيق أجمل ما في التماثل + +353 +00:26:11,790 --> 00:26:16,310 +الدقيق هنا هو المكون .. بحاجات اللي بنشوفه من + +354 +00:26:16,310 --> 00:26:21,150 +الشكل الخارجي، احنا بنشوف الـ capsule المكونة من + +355 +00:26:21,150 --> 00:26:25,510 +مين؟ من Capsomeres Capsomeres هي الوحدات البنائية + +356 +00:26:25,510 --> 00:26:30,590 +تخيلوا معايا التالية، معايا كبشة بانانير تابل جلول + +357 +00:26:30,590 --> 00:26:34,950 +أكيد عرفتوا الموسم هو ده موسم .. ماسكتهم أنا هيك + +358 +00:26:34,950 --> 00:26:40,900 +ورميتُهم على الحائط لما اصطدموا بالحائط وجههم بدأوا + +359 +00:26:40,900 --> 00:26:43,900 +يترتبوا لحالهم تلقائيًا واحد يطلع في وجه الثاني وفي + +360 +00:26:43,900 --> 00:26:47,400 +وجه الثاني واحنا بشكلي .. بعملش شكل هندسي معين + +361 +00:26:47,400 --> 00:26:52,880 +إيش ميزة .. إيش الميزة اللي أنا بحصدها أو هالجلول + +362 +00:26:52,880 --> 00:26:58,130 +كلهم حجم واحد صح؟ كلهم من نوع واحد، المصنع واحد + +363 +00:26:58,130 --> 00:27:01,730 +وبالتالي بيلزمنيش أكثر من gene، بدي gene واحد فقط + +364 +00:27:01,730 --> 00:27:06,510 +لغاية، gene واحد، يبقى القبق على الـ chromosome خف + +365 +00:27:06,510 --> 00:27:11,430 +ولا زاد، خف لما gene واحد مطلوب، هدأ إنهم يكونوا + +366 +00:27:11,430 --> 00:27:17,910 +نوع واحد، حجم واحد، طب إنهم مترتبوا لحالهم؟ بيعفونى + +367 +00:27:17,910 --> 00:27:22,150 +من مسألة إن أنا هصنع إنزيم تمسك الحقب وتحطها في + +368 +00:27:22,150 --> 00:27:25,730 +الحقب وتلزجها مظبوط أو لأ فبدل إن هم بيعملوا self + +369 +00:27:25,730 --> 00:27:29,430 +assembly يبقى كمان بيوفروا عليا energy بيوفروا + +370 +00:27:29,430 --> 00:27:32,490 +عليا كمان برضه الـ genome يعني مافيش داعي إن أنا + +371 +00:27:32,490 --> 00:27:36,230 +أصنع لهم إنزيمات ترتبهم مع بعض والـ CAPSOMERES ثبت + +372 +00:27:36,230 --> 00:27:39,990 +علميًا إنهم لما حطناها في test tube عملت self + +373 +00:27:39,990 --> 00:27:46,700 +assembly تجميع تلقائي بدون أي تدخل أو أي إنزيمات + +374 +00:27:46,700 --> 00:27:51,000 +وكأنك بتتكلم عن science fiction يعني عن .. عن خيال + +375 +00:27:51,000 --> 00:27:54,820 +علمي إن هذه الجزئيات بترتب لحدها وتكوّنها هذا + +376 +00:27:54,820 --> 00:27:59,560 +الشكل اللي سميناها precise geometric symmetry على + +377 +00:27:59,560 --> 00:28:05,260 +الرغم من صغرها وبساطتها إلا أنها بتحمل تعقيدات + +378 +00:28:05,260 --> 00:28:08,680 +كبيرة، ال repeating subunits اللي حكينا عنهم اللي + +379 +00:28:08,680 --> 00:28:13,280 +هم الكابسيومين هم اللي بيكونوا الكابسيت، تعالوا + +380 +00:28:13,280 --> 00:28:16,700 +تشوفوا السفينة في الفضاء هذه الحمراء، هذه الكورة + +381 +00:28:16,700 --> 00:28:20,740 +الدم الحمراء، وهذه البكتيريا اللي هي Titanic، + +382 +00:28:20,740 --> 00:28:24,380 +همناها؟ هذه هي إيش؟ السفينة.. السفينة الضخمة اللي + +383 +00:28:24,380 --> 00:28:29,020 +شايفينها، هذه ال eco line، بينما البكتيريا هذه بتش... + +384 +00:28:29,020 --> 00:28:32,020 +أو الفيروس، هذا بيشبه ال bullet shape + +385 +00:28:35,880 --> 00:28:39,860 +اللي هو طوب، تمام، ال block، وهذا ال bacteriophage + +386 +00:28:39,860 --> 00:28:43,400 +الشهير، عارفين من ال bacteriophage، تمام؟ و شوفوا + +387 +00:28:43,400 --> 00:28:47,880 +بعض الفيروسات الصغيرة، البعض طويل ورفيع، البعض طويل + +388 +00:28:47,880 --> 00:28:52,120 +وضخم، وهذا هو اللي مجلجل الدنيا هذه الأيام اللي + +389 +00:28:52,120 --> 00:28:56,440 +اسمه Ebola virus اللي هو بسموه worm-like، worm-like + +390 +00:28:56,440 --> 00:29:00,400 +يعني زي الدودة، عامل زي شكل الدودة، لكن لو جربنا + +391 +00:29:00,400 --> 00:29:04,880 +نركز أكثر، أكثر، أكثر هنشوف تفاصيل دقيقة رائعة جدا + +392 +00:29:04,880 --> 00:29:09,500 +وعملية ترتيب لل repeating subunits اللي سميناها + +393 +00:29:09,500 --> 00:29:14,780 +capsules بطريقة واحدة، من نتيجتها يا إما helical + +394 +00:29:14,780 --> 00:29:20,500 +حلزونية، يا إما icosahedral، إيش يعني icosahedral؟ + +395 +00:29:28,260 --> 00:29:32,020 +عشروني الوجوه، عشروني الوجوه، هنشوف صور كمان شوية + +396 +00:29:32,020 --> 00:29:37,220 +أدق، لكن خلينا نتأمل شوية في الحجوم على اللوحة + +397 +00:29:37,220 --> 00:29:41,920 +اللوحة بتعطينا إيهام بالحجوم تبعت الفيروسات + +398 +00:29:41,920 --> 00:29:46,300 +وبالتالي بتعطينا فكرة إن احنا بالفعل درسنا ورأيناهم + +399 +00:29:46,300 --> 00:29:50,700 +بالميكروسكوب الضوئي؟ سهل أو ما أبلعفش أشوفه كده + +400 +00:29:50,700 --> 00:29:55,970 +هيك أو ما أبلعفش أشوفه، آه يعني احنا حبينا في الصورة + +401 +00:29:55,970 --> 00:30:01,010 +هذه نعطي إيهام بالفروقات في الحجوم من أكبر فيروس زي + +402 +00:30:01,010 --> 00:30:05,210 +الإيبولا وال box virus، هذا بتبقى روض ضخم، هذا ال + +403 +00:30:05,210 --> 00:30:09,330 +box virus هو من أضخم الفيروسات لإن هو طول بعرض، مش + +404 +00:30:09,330 --> 00:30:12,310 +دي هنا بس، طول هنا، طول لإن هو أكثر شيء الـ ال + +405 +00:30:12,310 --> 00:30:17,570 +elongation هنا طويلة كثير ورفيع، لكن في الآخر + +406 +00:30:17,570 --> 00:30:21,900 +كلهم، كلهم كانوا يحتاجوا بجوش أو ممكن يقعدوا على ال + +407 +00:30:21,900 --> 00:30:24,940 +cell membrane أو ال cell wall تبع الإشريكية هي كلها + +408 +00:30:24,940 --> 00:30:29,640 +مؤشر على إنهم بالفعل they are small in size لكن + +409 +00:30:29,640 --> 00:30:32,520 +variable، variable في ال shapes و variable في ال + +410 +00:30:32,520 --> 00:30:37,020 +size، طيب السؤال الثاني، الصورة الثانية، تمثيل بسيط ل + +411 +00:30:37,020 --> 00:30:40,540 +two viruses، وراينا ال core، وال core اللي جوا ده إيش + +412 +00:30:40,540 --> 00:30:44,880 +بيكون، بيكون هنا يا إما DNA يا إما RNA، وهذا بيسميه + +413 +00:30:44,880 --> 00:30:48,540 +احنا كلّه collectively نوكليو كابسيد، نوكليو كابسيد + +414 +00:30:48,540 --> 00:30:52,160 +مع نوكليو كابسيد بيسميه نوكليو كابسيد، فأبسط فيروس + +415 +00:30:52,160 --> 00:30:55,760 +بتكون من نوكليو كابسيد، بعض الفيروسات يضاف إليها + +416 +00:30:55,760 --> 00:30:59,380 +اللي قلنا عنه ال envelope، تمام، فبنسميها envelope + +417 +00:30:59,380 --> 00:31:02,100 +viruses زي اللي قدامنا هاي ال envelope viruses + +418 +00:31:02,100 --> 00:31:06,280 +اللحظة طالع منها أشواك، بسميها spikes، هذه ال spikes + +419 +00:31:06,280 --> 00:31:10,760 +مكونة من glycoproteins، glycoproteins، و + +420 +00:31:10,760 --> 00:31:15,600 +بيختصروها GP، GP مهم جدا ال GP هذا جاء في الامتحان + +421 +00:31:15,600 --> 00:31:18,700 +عشان تكتبوه، جاء في الامتحان ال GP أو ال + +422 +00:31:18,700 --> 00:31:21,180 +glycoproteins لإنه في الآخر لما تدرسوا فيروسات إن + +423 +00:31:21,180 --> 00:31:25,120 +شاء الله هتعرفوا إن ال GP له دور مهم جدا جدا جدا + +424 +00:31:25,120 --> 00:31:28,460 +في عمل ال attachment to the cells، وأول خطوة من ال + +425 +00:31:28,460 --> 00:31:31,200 +viral infection هي ال attachment، بدونها ما فيش هنا + +426 +00:31:31,200 --> 00:31:36,480 +إمكانية ال attachment، كمان صورة بتعطينا أشكال + +427 +00:31:36,480 --> 00:31:43,880 +مختلفة ومتنوعة، طلعوا هنا very + +428 +00:31:43,880 --> 00:31:49,100 +very small، the smallest، وهذا اللي قلنا عنه ال box + +429 +00:31:49,100 --> 00:31:52,540 +virus، ال icosahedron، هاي عشروني الوجوه شايفينه هذا + +430 +00:31:52,540 --> 00:31:57,360 +اللي هو عبارة عن مثلثات، لكن في الآخر المثلثات هذه + +431 +00:31:57,360 --> 00:32:03,330 +بتعطيني شكل spherical، شكل spherical، هذا طبعا بيكون + +432 +00:32:03,330 --> 00:32:06,450 +إيه؟ Spiral، يبقى إيه؟ يا إما Spiral يا إما + +433 +00:32:06,450 --> 00:32:10,570 +icosahedral، في حالة ثالثة اللي هي Complex، ال + +434 +00:32:10,570 --> 00:32:15,690 +Complex غالبا بيكون مزيج بين الـ الـ الـ + +435 +00:32:15,690 --> 00:32:21,110 +icosahedral زي مثلا ال bacteriophage هذا + +436 +00:32:21,110 --> 00:32:22,330 +ال bacteriophage، شايفينه هو؟ + +437 +00:32:28,080 --> 00:32:31,840 +هذا الشيء بتاعته هي عبارة عن helical أو اسمه + +438 +00:32:31,840 --> 00:32:37,000 +complex أو أي شيء ليه انطبق عليه icosahedral أو + +439 +00:32:37,000 --> 00:32:41,560 +helical بنسميه برضه complex، إذا complex، helical + +440 +00:32:41,560 --> 00:32:43,000 +icosahedral + +441 +00:32:50,630 --> 00:32:54,790 +الـ genetic material is located internally and can be + +442 +00:32:54,790 --> 00:32:59,890 +either واحد single or double-stranded DNA or + +443 +00:32:59,890 --> 00:33:05,110 +single or double-stranded RNA، يبقى أكمنه عن أنها + +444 +00:33:05,110 --> 00:33:09,910 +دورة أربعة أربعة بدون أن نتكلم عن تفاصيل داخلية + +445 +00:33:09,910 --> 00:33:13,050 +positive، negative، double-stranded، single-stranded DNA + +446 +00:33:18,300 --> 00:33:21,120 +في ال RNA قلنا لكم في single .. في ال single + +447 +00:33:21,120 --> 00:33:25,380 +stranded في عندي positive strand أو positive sense + +448 +00:33:25,380 --> 00:33:26,460 +و negative sense + +449 +00:33:29,960 --> 00:33:32,580 +Have genetic materials composed of a single + +450 +00:33:32,580 --> 00:33:35,640 +-stranded DNA or of a single-stranded or double + +451 +00:33:35,640 --> 00:33:40,180 +-stranded RNA؟ هل درستم مخلوقات المادة الوراثية + +452 +00:33:40,180 --> 00:33:43,940 +تبعتها تتكون من single-stranded DNA قبل هيك؟ هل + +453 +00:33:43,940 --> 00:33:48,340 +درستم إن مخلوقات فيها المادة الوراثية هي single أو + +454 +00:33:48,340 --> 00:33:53,160 +double-stranded RNA فقط الفيروسات؟ إذن اليوم + +455 +00:33:53,160 --> 00:33:56,940 +هنتعرفنا على مجموعة من المخلوقات تمتلك unique + +456 +00:33:56,940 --> 00:34:01,670 +characters، صفات مميزة أو مميزة، إلا The nucleic + +457 +00:34:01,670 --> 00:34:05,630 +acids can be either linear or circular، يمكن أن + +458 +00:34:05,630 --> 00:34:13,370 +تكون ممتدة أو دائرة، ال DNA هو دائما مجموعة واحدة + +459 +00:34:13,370 --> 00:34:17,750 +ال DNA غالبا يكون جزء واحد، بينما ال RNA يمكن أن + +460 +00:34:17,750 --> 00:34:22,490 +تكون مجموعة واحدة أو في مجموعات مختلفة، الجملة اللي + +461 +00:34:22,490 --> 00:34:27,460 +بعدها بتوضح الجملة اللي قبلها، مثلا بقولنا both + +462 +00:34:27,460 --> 00:34:32,240 +influenza اللي طبعا رفيقة الدولة تبعنا منذ الصغر + +463 +00:34:32,240 --> 00:34:37,140 +وهذا أحد التفسيرات اللي الآن نتكلم لماذا ال + +464 +00:34:37,140 --> 00:34:42,060 +influenza قادرة على تغيير الجينات تبعتها بشكل + +465 +00:34:42,060 --> 00:34:49,760 +مستمر، لأن الجينوم تبعها segmented، مقطع بدل ما هو + +466 +00:34:49,760 --> 00:34:55,260 +قطعة طويلة، بيكون مقطع، هذا التقطيع إيش بيعمل؟ بيسهل + +467 +00:34:55,260 --> 00:34:59,680 +عملية التبادل، تخيلوا لما واحد اتبادل كروموزوم طويل + +468 +00:34:59,680 --> 00:35:03,520 +تمام مع فيروس ثاني أو قطعة من فيروس .. من .. من + +469 +00:35:03,520 --> 00:35:06,220 +فيروس .. من .. من جينوم، أعتقد القطعة بتكون إيه؟ + +470 +00:35:06,220 --> 00:35:10,180 +أجزاء خاصة، ده راحنا إن في عندها ثمان قطع، ال + +471 +00:35:10,180 --> 00:35:14,460 +influenza عندها ثمان قطع، لو اثنين influenza غرب عن + +472 +00:35:14,460 --> 00:35:19,200 +بعض، غرب يعني different strands دخلوا خلية واحدة + +473 +00:35:19,200 --> 00:35:19,920 +مرة واحدة + +474 +00:35:23,230 --> 00:35:27,590 +عملية ال replication أو تصنيع المادة الوراثية + +475 +00:35:27,590 --> 00:35:32,250 +بتبقى حاجة بيسموها packaging، تعبئة، تكوّر الكابسيد + +476 +00:35:32,250 --> 00:35:35,750 +بنعبي فيه المادة الوراثية، خلال عملية التعبية بيصير + +477 +00:35:35,750 --> 00:35:41,010 +في exchange، تبادل، ففيروس X بياخد من Y قطعة ولو + +478 +00:35:41,010 --> 00:35:45,530 +قطعة واحدة وفيروس Y بياخد من X قطعة وطلعنا new + +479 +00:35:45,530 --> 00:35:51,140 +viruses، قد تكون ال new viruses بتموت، لكن كل فترة و + +480 +00:35:51,140 --> 00:35:53,560 +فترة بطلعنا سلالة ناجحة .. ناجحة من وجود نظرها + +481 +00:35:53,560 --> 00:35:56,540 +مهمة .. آه .. حتى ناجحة من وجود نظرها مين؟ اللي + +482 +00:35:56,540 --> 00:36:01,760 +بتموت .. وبتعملنا مشكلة كبيرة في كوكب الأرض زي + +483 +00:36:01,760 --> 00:36:04,680 +ال influenza الطيور وال influenza الخنازير وما + +484 +00:36:04,680 --> 00:36:08,660 +شابه ذلك .. خدوا بالكم تاريخ ال influenza شهير .. + +485 +00:36:08,660 --> 00:36:14,720 +يعني في 1918 قتلت ال influenza الإسبانية أربعين + +486 +00:36:14,720 --> 00:36:18,180 +مليون إنسان، وبجوا زمان عدد السكان .. مش ده اليوم + +487 +00:36:18,680 --> 00:36:22,360 +الأربعين مليون حالة كبيرة، الأربعين مليون هلولة هم + +488 +00:36:22,360 --> 00:36:25,520 +أكبر من عدد من قتل في الحرب العالمية الأولى + +489 +00:36:25,520 --> 00:36:28,240 +والثانية والحرب الفيتنامية والحرب الأمريكية + +490 +00:36:28,240 --> 00:36:32,820 +الأهلية، إيش رأيكم؟ عشانك لما كانوا الناس خايفين + +491 +00:36:32,820 --> 00:36:36,720 +لما قادت influenza الطيور كان في مبرر، طبعا البعض + +492 +00:36:36,720 --> 00:36:41,860 +بيميل إلى نظرية المؤامرة يقول إن شركات الأدوية و + +493 +00:36:41,860 --> 00:36:47,750 +السياسة إنهم بيميلوا الناس في الـ بالـ بهذا الأخبار + +494 +00:36:47,750 --> 00:36:54,090 +لأ احنا بنقول إن لأول مرة البشر يمتلكوا فرصة إنهم + +495 +00:36:54,090 --> 00:36:58,990 +يستعدوا لوباء قادم، يستعدوا .. هذه الفرصة الوحيدة + +496 +00:36:58,990 --> 00:37:01,230 +اللي على مر التاريخ إن احنا نستعد لهذا الوباء + +497 +00:37:01,230 --> 00:37:08,310 +لأنها ترصد جيدا و .. و المشكلة إن لما الـ الـ + +498 +00:37:08,310 --> 00:37:14,590 +الإعلام نشر المعلومات، الناس صار عندها خوف كبير و + +499 +00:37:14,590 --> 00:37:17,870 +بعد ما الموجة راحت صاروا يقولوا يبقى هو مش .. مش + +500 +00:37:17,870 --> 00:37:21,290 +صحيح، يبقى كذابين، يبقى مش صادقين، يبقى بعطونا + +501 +00:37:21,290 --> 00:37:27,010 +بيخوفونا شوفوا ال .. الفكرة كانت مع إنفلوانزا + +502 +00:37:27,010 --> 00:37:30,170 +الطيور .. إنفلوانزا الطيور بيسموها highly + +503 +00:37:30,170 --> 00:37:34,590 +pathogenic strain إيش يعني highly pathogenic يعني + +504 +00:37:34,590 --> 00:37:37,980 +شدة الإمراض طبيعتها عالية جدا، نسبة الوفيات بيبقى + +505 +00:37:37,980 --> 00:37:40,660 +فيها ثلاثة وستين في المئة، ثلاثة وستين في المئة يعني لو + +506 +00:37:40,660 --> 00:37:45,180 +ألف أصيب، ثلاثة وستماية وثلاثين واحد بيموتهم + +507 +00:37:45,180 --> 00:37:50,820 +تمام؟ إيش الميزة السبعة؟ الميزة إنه ما بتنتقلش من + +508 +00:37:50,820 --> 00:37:54,460 +إنسان لإنسان، لازم من الطير لمين؟ للإنسان + +509 +00:37:54,460 --> 00:37:59,640 +وبالتالي عدد حوالي ست أو سبعة سنوات، ست سنوات بتلفلف في + +510 +00:37:59,640 --> 00:38:05,300 +الدنيا، عدد من أصيب إنسان زي، زي الإنفلوانزا ما تجاوز + +511 +00:38:05,300 --> 00:38:07,560 +ولا ألف حالة، تعالوا على الجهة الثانية، نروح على الـ + +512 +00:38:07,560 --> 00:38:12,120 +influenza الخنازير، الـ influenza الخنازير أصبحت + +513 +00:38:12,120 --> 00:38:14,360 +أكثر من الـ influenza الموسمية، وصنفت عليها الـ + +514 +00:38:14,360 --> 00:38:20,360 +influenza الموسمية، نسبة الوفيات فيها 0.005 يعني + +515 +00:38:20,360 --> 00:38:24,900 +أقل من الـ influenza الموسمية نفسها، يعني من المليون + +516 +00:38:24,900 --> 00:38:28,760 +بتموت حالة، لكن خدوا بالكم إن شاء الله لو التقى + +517 +00:38:28,760 --> 00:38:35,150 +الخنازير مع الطيور وتبادلوا، هذا أعطل، هذا المفضل على + +518 +00:38:35,150 --> 00:38:40,250 +الـ human transmission واحتفظ بالـ highly pathogenic + +519 +00:38:40,250 --> 00:38:45,470 +اللي 63% القتل، معناه اللي حكيته حيصير فيه سهولة + +520 +00:38:45,470 --> 00:38:48,990 +الانتقال، حيصير فيه قتل، تخيلوا لما يصابوا + +521 +00:38:48,990 --> 00:38:52,950 +المليارات من البشر بالـ influence الطيور المحسنة + +522 +00:38:52,950 --> 00:38:53,570 +المعدلة + +523 +00:38:57,490 --> 00:39:02,370 +الموجودة، إن يصير هذا التبادل بين الفيروسات وينتج + +524 +00:39:02,370 --> 00:39:05,770 +عنها سلالة من هذا الصنف، فلأنا بقى ناخد + +525 +00:39:05,770 --> 00:39:09,510 +المواضيع ناخدها بمنطقية وعقلانية، الفيروسات عندنا + +526 +00:39:09,510 --> 00:39:14,010 +المقدرة، وكل عشر سنوات، إذا بتلاحظوا إلى خمسة عشر سنة + +527 +00:39:14,010 --> 00:39:17,810 +بيطلع عندنا سلالات جديدة، سلالات زي مثلًا الـ SARS + +528 +00:39:19,280 --> 00:39:22,420 +حيطلع إن آخر شيء اللي هو الـ SARS أو الكورونا تبعه + +529 +00:39:22,420 --> 00:39:26,140 +من الـ east هذا اللي هو بالضبط السعودية، جاعله فيه + +530 +00:39:26,140 --> 00:39:29,860 +إن كل فترة وفترة بيطلع سلالات جديدة، أما مقاومة للـ + +531 +00:39:29,860 --> 00:39:33,680 +drugs أو إنها محسنة أو عندها بعض الصفات اللي .. + +532 +00:39:37,730 --> 00:39:41,930 +كل الفيروسات بتحتوي على single copy، كلمة single + +533 +00:39:41,930 --> 00:39:45,970 +copy إيش يعني؟ نسخة .. نسخة .. نسخة .. نسخة .. + +534 +00:39:45,970 --> 00:39:46,270 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +535 +00:39:46,270 --> 00:39:46,410 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +536 +00:39:46,410 --> 00:39:46,930 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +537 +00:39:46,930 --> 00:39:47,670 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +538 +00:39:47,670 --> 00:39:58,150 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +539 +00:39:58,150 --> 00:40:01,690 +نسخة + +540 +00:40:01,690 --> 00:40:05,650 +.. نُسَالخلية العادية بيسميها diploid والخلية + +541 +00:40:05,650 --> 00:40:08,970 +الجنسية بيسميها haploid اللي فيها نصف عدد، نصف عدد + +542 +00:40:08,970 --> 00:40:12,390 +الكروموزومات، نصف عدد الكروموزومات، إيه شهر ده في عدد + +543 +00:40:12,390 --> 00:40:15,290 +الكروموزومات؟ يعني من كل كروموزوم فيه two copies، + +544 +00:40:15,290 --> 00:40:19,510 +فـ diploid و haploid، كل الفيروسات are haploid، + +545 +00:40:19,510 --> 00:40:25,360 +معادا الـ retrovirus، نعم، الـ retro virus بيكون عبارة + +546 +00:40:25,360 --> 00:40:29,040 +عن diploid، يعني فيروس الـ aids اللي بينتمي له الـ + +547 +00:40:29,040 --> 00:40:33,220 +retro viruses بيحتاج على two copies من الجينوم، two + +548 +00:40:33,220 --> 00:40:37,980 +copies من الجينوم، هاي الـ exception في السطر الأخير + +549 +00:40:37,980 --> 00:40:42,460 +من اللوحة، بتكلم عن the exception is the retrovirus + +550 +00:40:42,460 --> 00:40:47,060 +family whose members have two copies of their RNA + +551 +00:40:47,060 --> 00:40:52,540 +genome is that they are diploid، Viral capsid and + +552 +00:40:52,540 --> 00:40:55,960 +symmetry، وهذه اللي أنا حدثت فيها المحاضرة اليوم أو + +553 +00:40:55,960 --> 00:40:59,640 +بعد الصور تبعتها، The nucleic acid is surrounded by + +554 +00:40:59,640 --> 00:41:02,680 +a protein coat called a capsid، وبينها الـ capsid + +555 +00:41:02,680 --> 00:41:06,620 +بتكون من subunits سميناها الـ capsomeres، وأنا + +556 +00:41:06,620 --> 00:41:09,660 +الصحيح الكلام اللي قلته على السبورة وشرحته الآن إن + +557 +00:41:09,660 --> 00:41:13,880 +كل الـ capsomere بتكون من one or several proteins + +558 +00:41:13,880 --> 00:41:17,280 +can be seen only in the electron microscope as + +559 +00:41:17,280 --> 00:41:21,900 +spherical particles، Sometimes with a central hole + +560 +00:41:21,900 --> 00:41:25,920 +يعني مفردة، يبدو إن الفراغ هذا يعني الـ protein اللي + +561 +00:41:25,920 --> 00:41:31,680 +مكوّن أو اللي هو الـ hole هو اللي بيساعد الـ .. الـ + +562 +00:41:31,680 --> 00:41:35,580 +.. الـ .. الـ capsules على ما يسمى بالـ self assembly + +563 +00:41:35,580 --> 00:41:41,880 +اللي هو التجميع، Is there the structure composed of + +564 +00:41:41,880 --> 00:41:46,880 +the nucleic acid genome and the capsid protein is + +565 +00:41:46,880 --> 00:41:49,040 +called the nucleocapsid، وعن هذا الكلام حكيناه + +566 +00:41:49,040 --> 00:41:52,640 +نوكليو كابسيد، قلنا عنها إنه أبسط فيروس مكوّن من + +567 +00:41:52,640 --> 00:41:56,280 +نوكليو كابسيد، The arrangement of capsomers gives + +568 +00:41:56,280 --> 00:41:59,060 +the virus structure its geometric symmetry، وإن + +569 +00:41:59,060 --> 00:42:07,300 +السيمتري تبعته اللي هي هذه إيكوزاهيدرال، والتاني + +570 +00:42:07,300 --> 00:42:10,620 +اللي يقول عنها helical، وقلنا هي في مثلها إنها لأ + +571 +00:42:10,620 --> 00:42:16,240 +هي مثلها إنها إيكوزا وهي naked، naked يعني ما لهوش + +572 +00:42:16,240 --> 00:42:20,340 +envelope، و envelope يبقى عكس envelope بنسميها أما + +573 +00:42:20,340 --> 00:42:25,260 +non envelope أو بنسميها naked غير، وأنا شرحت وأرجو + +574 +00:42:25,260 --> 00:42:29,000 +أنه يظلوا في الذاكرة إن الـ envelope إلو ميزة لكن + +575 +00:42:29,000 --> 00:42:34,500 +برضه إلو عيب، والعيب هذا يعني من .. بعدي، It + +576 +00:42:34,500 --> 00:42:38,140 +confers in أو it confirms instability of the + +577 +00:42:38,140 --> 00:42:38,860 +virus in + +578 +00:42:44,300 --> 00:42:47,740 +هذه اللوحة هي الكلام اللي حكيته عن الكبسومير، إيش + +579 +00:42:47,740 --> 00:42:50,780 +الـ advantage of building the virus particle from + +580 +00:42:50,780 --> 00:42:53,700 +identical protein subunits، أنا قلت الـ glues، + +581 +00:42:53,700 --> 00:42:56,940 +مظبوط اللي هو identical، واحد، It reduces the need + +582 +00:42:56,940 --> 00:42:59,920 +for genetic information، أعتقد كلمة genetic + +583 +00:42:59,920 --> 00:43:03,100 +information يعني مادة وراثية، يعني بدل ما نحمل .. + +584 +00:43:03,100 --> 00:43:06,400 +تخيل لو إنه مصنوعة من .. من قطع حمرا أو زرقا أو + +585 +00:43:06,400 --> 00:43:10,000 +صفرا أو أحجام مختلفة أو كده، فمعنى الحكي إنه كل + +586 +00:43:10,000 --> 00:43:13,540 +قطع هان تكون فيها جين مختلف، فهذا هيزيد من إيه؟ + +587 +00:43:13,540 --> 00:43:18,420 +فبتقلل من كفاءة الـ virus، It promotes self-assembly + +588 +00:43:18,420 --> 00:43:25,040 +يبقى بيحسن اللي هو أو بيساعد على التجميع التلقائي + +589 +00:43:25,040 --> 00:43:29,310 +أو الـ .. الجملة .. الفقرة الأخيرة بتقول إن في + +590 +00:43:29,310 --> 00:43:36,370 +الواقع تم تكوين فيروس كامل دون وجود خلية، فقط أخد + +591 +00:43:36,370 --> 00:43:38,910 +الـ capsomers وحطوها في test tube وبالفعل اتكون + +592 +00:43:38,910 --> 00:43:43,410 +فيروس كامل، وعندها اللوحة إن شاء الله من المرة + +593 +00:43:43,410 --> 00:43:43,790 +القادمة diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IzxXy5S0TBo_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IzxXy5S0TBo_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..861e7f0aa8b912ec6600f843dae2dce351640602 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/IzxXy5S0TBo_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 4806, "start": 20.86, "end": 48.06, "text": "بسم الله الرحمن الرحيم استكملنا لموضوع كتير مهم بدأنا فيه اللي هو الموضوع المضادات الحيوية حدثنا عن تعريفات حدثنا عن تصنيفات حدثنا عن mechanisms of action و بعدين انتقلنا لل resistance و بعدين انتقلنا ل how to test these antimicrobials في المعمل و خدنا ال disk diffusion و ال broad solution و عرفنا مصطلحات زي ال MIC و ال MBC", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 44713, 24793, 1211, 8315, 32767, 2407, 11242, 45367, 9122, 2655, 13546, 3714, 16095, 47525, 10721, 8315, 8978, 3224, 13672, 1829, 31439, 9673, 2407, 11242, 45367, 9673, 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حكيناه نوكليو كابسيد قلنا عنها أنه أبسط فيروس مكوّن من نوكليو كابسيد the arrangement of capsimers gives the virus structure its geometric symmetry وإن السيمتري تبعته اللي هي هذه إيكوزا هيلرال", "tokens": [474, 264, 13855, 327, 7944, 307, 1219, 264, 14962, 24035, 1878, 327, 4032, 3615, 1863, 23758, 2423, 28820, 10943, 11331, 4117, 1829, 8315, 3224, 8717, 2407, 4117, 20292, 2407, 9122, 16758, 3794, 25708, 12174, 1211, 8315, 18871, 11296, 14739, 3224, 5551, 3555, 3794, 9566, 8978, 32887, 3794, 3714, 4117, 2407, 11703, 1863, 9154, 8717, 2407, 4117, 20292, 2407, 9122, 16758, 3794, 25708, 264, 17620, 295, 13855, 332, 433, 2709, 264, 5752, 3877, 1080, 33246, 25440, 4032, 28814, 1863, 21136, 32640, 2655, 16572, 6055, 3555, 34268, 3224, 13672, 1829, 39896, 29538, 11933, 1829, 4117, 2407, 11622, 995, 8032, 26895, 2288, 6027], "avg_logprob": -0.3443688154220581, "compression_ratio": 1.5274261603375527, "no_speech_prob": 0.0, "words": [{"start": 2505.26, "end": 2505.72, "word": "and", 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+المعمل و خدنا ال disk diffusion و ال broad + +7 +00:00:44,260 --> 00:00:50,010 +solution و عرفنا مصطلحات زي ال MIC و ال MBCووصلنا + +8 +00:00:50,010 --> 00:00:56,170 +للبرتقالة هذه اللي قدامنا اللي فيها action و + +9 +00:00:56,170 --> 00:00:59,170 +reaction واحد بتفعل الأمام و واحد بتفعل الخلف و + +10 +00:00:59,170 --> 00:01:04,050 +مكانك سامة وهذه تعبير عن علاقة ما بين two + +11 +00:01:04,050 --> 00:01:11,580 +antibiotics واحد بيثبت عمل الآخرو احنا ممكن درسنا + +12 +00:01:11,580 --> 00:01:17,020 +قبل ذلك حاجة اسمها تآذر، ان واحد بيساعد عمل الآخر، + +13 +00:01:17,020 --> 00:01:20,140 +هذا الكلام درسناه لما كلمنا عن ال camp test في ال + +14 +00:01:20,140 --> 00:01:23,580 +group B streptococci ليه streptococcus a galactia، + +15 +00:01:23,580 --> 00:01:27,460 +بإن واحد هيموليسين بيعمل تآذر، synergism مع + +16 +00:01:27,460 --> 00:01:31,300 +الهيموليسين تبع الاستافيلوكوكاس اورس، فبزيد كمية + +17 +00:01:31,300 --> 00:01:33,180 +الهيموليسيس و يعطينا ال arrowhead shape إذا + +18 +00:01:33,180 --> 00:01:36,610 +باكرين، ال arrowhead shapeيبقى العلاقات اللى + +19 +00:01:36,610 --> 00:01:39,510 +بنتكلم عنها اليوم أربع علاقات هو يا صبورة و أطول + +20 +00:01:39,510 --> 00:01:44,090 +تلاتة بنتكلم عن أربع علاقات العلاقة الأولى + +21 +00:01:44,090 --> 00:01:47,330 +indifference indifference يعني مش فارقة يجب أن + +22 +00:01:47,330 --> 00:01:53,390 +نطلع اللوحة طول الوقت و نشوف مستوى البكتيريا جاهز + +23 +00:01:53,390 --> 00:01:58,110 +بيطلع لما يكون فيه no drugs صح بيكبر جاهز لو حطينا + +24 +00:01:58,110 --> 00:02:02,430 +drug A مستوى البكتيريا بيكون لعندها أجل لكن لسه + +25 +00:02:02,430 --> 00:02:06,970 +فيه نمولو حطينا drug B شوفوا كتير بنزل .. كتير + +26 +00:02:06,970 --> 00:02:11,190 +بنزل .. لكن لو حطينا التنين مع بعض .. لحظه أنه + +27 +00:02:11,190 --> 00:02:15,690 +مافيش اختلاف بين drug B لحاله و بين A و B فبالتالي + +28 +00:02:15,690 --> 00:02:20,830 +A لم يضف ل B فبالتالي النتيجة in difference .. + +29 +00:02:20,830 --> 00:02:25,430 +مافيش فرق .. مافيش فرق الشيء التاني .. اتطلع هذا + +30 +00:02:25,430 --> 00:02:31,680 +.. هو drug اتطلعهالدراج C جريد من يعني ان ال + +31 +00:02:31,680 --> 00:02:37,040 +activity لها ضعيفة جدا خالص الدراج D قطر البكتيري + +32 +00:02:37,040 --> 00:02:41,860 +لكن من مستوى معين لكن لما حطينا الدراج C مع دي + +33 +00:02:41,860 --> 00:02:46,040 +شوفوا كيف التأثير رائع جدا أكبر ما يكون وبالتالي + +34 +00:02:46,040 --> 00:02:49,820 +يجب ان نقول انها 1 زاد 1 ساوي 5 عشرة مش 1 زاد 1 + +35 +00:02:49,820 --> 00:02:53,100 +ساوي اتنين في ده الحالة في ال antagonism اللي هي + +36 +00:02:53,100 --> 00:03:02,180 +التالتة دراج نو دراجE لحظة ودرج F هي درج E وهي درج + +37 +00:03:02,180 --> 00:03:07,820 +F درج F ممتاز كده خفض لما حطينا معاه E قلل من ال + +38 +00:03:07,820 --> 00:03:11,380 +effect هو يبقى هذا بالفعل زي البرتقال اللي شفناها + +39 +00:03:11,380 --> 00:03:15,840 +واحد بيشتغل وواحد بيخرّب الشغل فهو اللي بيسميه + +40 +00:03:15,840 --> 00:03:17,800 +احنا antagonism + +41 +00:03:21,840 --> 00:03:25,120 +الان الرابع اللي بيقول عنها addition واحد زائد + +42 +00:03:25,120 --> 00:03:29,560 +واحد يساوي اتنين واحد بقى يقتل عشرة و اتنين بيقتل + +43 +00:03:29,560 --> 00:03:33,440 +عشرة الان لما أضفناهم مع بعض يقتلوا عشرين هذا + +44 +00:03:33,440 --> 00:03:37,100 +simple mathematics مثلا مي addition طيب اللي مش + +45 +00:03:37,100 --> 00:03:41,440 +مضكوه على اللوحة و في المحاضرة بدنا نكتبه كتاب ان + +46 +00:03:41,440 --> 00:03:45,380 +شاء الله اكتبوا الان Seidel زائد Seidel + +47 +00:03:48,940 --> 00:03:53,520 +حطه C زائد C يعني جملة + +48 +00:03:53,520 --> 00:03:59,140 +جديدة static زائد static التالتة + +49 +00:03:59,140 --> 00:04:03,860 +الجملة التالتة اللي هي sidal زائد static الأولى + +50 +00:04:03,860 --> 00:04:08,820 +بلد for sure يعني قاعدة الأولى اللي بعطينا + +51 +00:04:08,820 --> 00:04:13,420 +synergism التانية + +52 +00:04:13,420 --> 00:04:19,020 +اللي هي static مع static بتعطيناAddition أو + +53 +00:04:19,020 --> 00:04:25,840 +Indifference Addition أو Indifference التالتة + +54 +00:04:25,840 --> 00:04:30,840 +Antagonism لأن أقولكوا كيف يصير Antagonism بالعرب + +55 +00:04:30,840 --> 00:04:35,180 +البسيط بالعربي البسيط فاكرين ال Antibiotic كيف + +56 +00:04:35,180 --> 00:04:40,720 +تشتغل؟الانتيباتك بتشتغل على ال active bacteria لما + +57 +00:04:40,720 --> 00:04:45,120 +شوفنا ال cell wall كنا ال cross link يمنع مش + +58 +00:04:45,120 --> 00:04:50,360 +بيتجسجس بيمنع طب هو لما يكون already موجود يبقى + +59 +00:04:50,360 --> 00:04:55,220 +مانا فيش امكانية لانتيباتك ان تعمل اي حاجة + +60 +00:04:55,220 --> 00:05:00,110 +وبالتاليفقط bacteria actively growing are affected + +61 +00:05:00,110 --> 00:05:03,770 +طب البكتيريا static هيوقف نشاط البكتيريا يبقى + +62 +00:05:03,770 --> 00:05:06,430 +البكتيريا صاحبة اللي قبل يكون معاه مش هيقتل + +63 +00:05:06,430 --> 00:05:08,710 +البكتيريا وهذا اللي بيصير فيه أننا كنا فيه + +64 +00:05:08,710 --> 00:05:12,910 +antagonism هذا الكلام مهم جدا ليش؟ لأنه + +65 +00:05:12,910 --> 00:05:18,610 +practically ممكن الطبيب يوصف للمريض two drugsإذا + +66 +00:05:18,610 --> 00:05:21,870 +واحد منهم أستاتيك و واحد منهم صيدل هيصير أن ال + +67 +00:05:21,870 --> 00:05:25,150 +energy الأهم اللي هو ال antagonism و بالتالي بدل + +68 +00:05:25,150 --> 00:05:27,770 +ما نزيد التأثير و أنتوا عارفين بنعطي two + +69 +00:05:27,770 --> 00:05:32,110 +antibiotics حتى نحسن من ال .. ال .. القدرة + +70 +00:05:32,110 --> 00:05:35,250 +الألاجية لل antibiotic و نقصر الفترة و الاخرى لكن + +71 +00:05:35,250 --> 00:05:38,070 +في هذا الحالة احنا بنكون يعني أسأنا الاختيار + +72 +00:05:40,400 --> 00:05:45,880 +الصور اللي قدامنا مش كتير واضحة لكن بدنا نحاول ان + +73 +00:05:45,880 --> 00:05:49,840 +نطلع منها .. لأ كويسة ميحة شايفين الدائرة اللي .. + +74 +00:05:49,840 --> 00:05:53,640 +اللي .. اللي بالخط المجزق وراها على طول هنا فيه + +75 +00:05:53,640 --> 00:06:00,400 +bacterial growth صح؟ لكن استكمال الدائرة المفروض + +76 +00:06:00,400 --> 00:06:03,280 +ان انتوا عارفين ان الظنب النامشي ليش بتكون دائرية + +77 +00:06:03,280 --> 00:06:07,760 +لأن القرص دائر و ال diffusion دائر ليش في المنطقة + +78 +00:06:07,760 --> 00:06:14,230 +هذهاللي هي بعد قطر الدائرة مشي بكتيريا ال + +79 +00:06:14,230 --> 00:06:17,970 +antibiotic التاني ما هو هي دائرته كل واحد هيحدود + +80 +00:06:17,970 --> 00:06:23,510 +المفروض هيحدود الإفتراضية لإن التقاء X مع Y عمل + +81 +00:06:23,510 --> 00:06:26,550 +سيناريز انفزاد من قدرة القتل في المنطقة اللي بين + +82 +00:06:26,550 --> 00:06:31,370 +التاني لأنه خد بالكوا هذه هي المنطقة التقاء ال two + +83 +00:06:31,370 --> 00:06:35,290 +antibiotics هنا antibiotic الأولاني مش هيكون موجود + +84 +00:06:35,290 --> 00:06:41,940 +صح؟و في المنطقة التانية فقط في منطقتها المنتصف إذا + +85 +00:06:41,940 --> 00:06:45,120 +ممكن احنا نشوف ال scenario زي اللي ممكن نعمل + +86 +00:06:45,120 --> 00:06:48,440 +sensitivity testing بال disk diffusion + +87 +00:06:51,360 --> 00:06:54,000 +طيب the future of chemotherapeutic areas احنا + +88 +00:06:54,000 --> 00:06:58,000 +عملنا صورة سوداء قاطمة على موضوع ال resistance و + +89 +00:06:58,000 --> 00:07:01,760 +انه و لسه من بارحة كنا بنكتب في تقرير scientific + +90 +00:07:01,760 --> 00:07:06,020 +من جزء من الشغل اللي بنشتغله في البحر تقرير قاطم + +91 +00:07:06,020 --> 00:07:10,040 +الصحي يعني ال percentage of resistance لل + +92 +00:07:10,040 --> 00:07:13,380 +enterococcus للفانكومايسين في البيئة عمالة ترتفع + +93 +00:07:13,380 --> 00:07:19,520 +بشكل مخيفطب هل هناك من مستقبل مشرق في هذا المجال؟ + +94 +00:07:19,520 --> 00:07:23,060 +هل هناك أمل يعني الباحثين بيشتغلوا عليه مثلا؟ + +95 +00:07:23,060 --> 00:07:27,760 +الواقع أنه كلمة مستقبل مستقبل في زماننا اللي احنا + +96 +00:07:27,760 --> 00:07:33,480 +فيه هذا كلمة فاضفاضة ولا تعني يعني بدقة أن احنا + +97 +00:07:33,480 --> 00:07:36,300 +بنتكلم عن مستقبل قريب ولا بعيد لأن الأحداث + +98 +00:07:36,300 --> 00:07:41,580 +المتلاحقة التطوراتمش بطيئة زي القرون الماضية + +99 +00:07:41,580 --> 00:07:44,340 +تتطورت انسانية جدا، من كان يتخيل إن الراديو اللي + +100 +00:07:44,340 --> 00:07:47,260 +كان حجمه الجد يصير تطبيق application في ال mobile + +101 +00:07:47,260 --> 00:07:51,580 +تبعنا، تمام؟ و ليس يعني جهاز ضخم، التلفزيون كذلك + +102 +00:07:51,580 --> 00:07:55,040 +الأمر، الحواسيب وسرعتها، الموبايلات نفسها كيف + +103 +00:07:55,040 --> 00:08:00,030 +تتطورت؟فما نقول ان ال future قد يكون already + +104 +00:08:00,030 --> 00:08:03,950 +passed يعني صار passed في بعض اللحظات لكن دائما + +105 +00:08:03,950 --> 00:08:08,570 +هناك frontiers يعني مجالات لم يطرقها الإنسان أو + +106 +00:08:08,570 --> 00:08:12,890 +طرقها ورد فيها باساس أمل فبيشجع الناس دايما انه + +107 +00:08:12,890 --> 00:08:16,000 +يعني يطرقوا و يبحثوا في هذه المجالاتمن هذه + +108 +00:08:16,000 --> 00:08:20,180 +المجالات مجالات درسنا احنا حزمة بيبتايت انتيبايتكس + +109 +00:08:20,180 --> 00:08:23,140 +بيبتايت انتيبايتكس أخدنا واحد اسمه بيبتايت بيبتايت + +110 +00:08:23,140 --> 00:08:25,980 +بيبتايت بيبتايت بيبتايت و أخدنا واحد اسمه جلايكو + +111 +00:08:25,980 --> 00:08:26,960 +بيبتايت جلايكو بيبتايت جلايكو بيبتايت جلايكو + +112 +00:08:26,960 --> 00:08:28,000 +بيبتايت جلايكو بيبتايت جلايكو بيبتايت جلايكو + +113 +00:08:28,000 --> 00:08:28,380 +بيبتايت جلايكو بيبتايت جلايكو بيبتايت جلايكو + +114 +00:08:28,380 --> 00:08:32,860 +بيبتايت جلايكو بيبتايت جلايكو + +115 +00:08:32,860 --> 00:08:35,580 +بيب + +116 +00:08:36,040 --> 00:08:41,060 +فكويس إنه ندور على peptide احنا نصنعها، يعني نحاول + +117 +00:08:41,060 --> 00:08:43,840 +نصنع peptides جديدة بالهندسة الوراثية اللي هو سهل + +118 +00:08:43,840 --> 00:08:48,220 +إنه نعمل ترتيبة لpeptides عن طريق إنه نصنع DNA و + +119 +00:08:48,220 --> 00:08:53,420 +بعدين ال RNA، دي كمان شغل تاني الحيوانات اللي + +120 +00:08:53,420 --> 00:09:00,090 +عايشة و بتحتكبيعني بيئات احنا .. احنا يعني الإنسان + +121 +00:09:00,090 --> 00:09:04,630 +مستوى النظافة عنده عالي جدا لكن في بيئات الحيوانات + +122 +00:09:04,630 --> 00:09:08,470 +زي أكلمة مضال خنازير مثلا على سبيل المثال أو + +123 +00:09:08,470 --> 00:09:12,330 +الأسماك زي سماكة القرش في البحر بتتناول GF و + +124 +00:09:12,330 --> 00:09:15,470 +بتتناول فمليانة بتكون بكتيريا فغالبا بيكون في + +125 +00:09:15,470 --> 00:09:21,460 +أجسامها مركبات لمكافحة فمثلاالسكورامين أخدوه ك + +126 +00:09:21,460 --> 00:09:25,680 +antibiotic من أجسام أسماك القرخ، هو ال sharks، فيه + +127 +00:09:25,680 --> 00:09:29,240 +ال .. ال protagreen من ال pigs، اللي هو الخنازير + +128 +00:09:29,240 --> 00:09:31,900 +أكرا و مقوم الله، و الحلس اللي ما عندك بتحكوله، + +129 +00:09:31,900 --> 00:09:38,930 +المجانين من ال frogs، تمام؟الان طبعا هذه أمثلة و + +130 +00:09:38,930 --> 00:09:42,930 +ليست قائمة حصرية يعني حتى البندورة الطماطم تمام + +131 +00:09:42,930 --> 00:09:46,950 +فيها تماتين مركبات برضه ايه بتاعة مقلة لكن إذا + +132 +00:09:46,950 --> 00:09:51,590 +بتلاحظوا ال tomato برضه بتعفن المعنى الآخر انه مش + +133 +00:09:51,590 --> 00:09:56,270 +لكل إشي مش لكل إشي أو الكميات بتكون قليلة فاحنا + +134 +00:09:56,270 --> 00:10:01,030 +ندرس إمكانية تحوير هذه المركبات أو إمكانية ال + +135 +00:10:01,030 --> 00:10:03,990 +synthesis .. ال synthesis إلها بمعنى إنه ننتجها + +136 +00:10:03,990 --> 00:10:08,500 +بكميات كبيرةالاخيرة حلوة تقنية كويسة اسمها + +137 +00:10:08,500 --> 00:10:12,600 +antisense technology أو antisense agents ورقصه + +138 +00:10:12,600 --> 00:10:17,720 +معايا في الهندوسية دائما احنا ال protein هو اللغة + +139 +00:10:17,720 --> 00:10:21,080 +اللي بتكلم فيها الخلية بسميها ال phenotype و + +140 +00:10:21,080 --> 00:10:25,620 +المادة الوراقية ال genotype يعبر ال gene عن نفسه + +141 +00:10:25,620 --> 00:10:30,740 +بإنتاج ال protein بسميها gene expressionعبر ال + +142 +00:10:30,740 --> 00:10:34,600 +gene expression في مرحلة بسيطة بتحول ال DNA إلى أو + +143 +00:10:34,600 --> 00:10:39,580 +بيصيله transcription بتطلع منه mRNA ال mRNA لو أنا + +144 +00:10:39,580 --> 00:10:45,820 +جيبت قطعة قطعة to complement the RNA إيش هيصير + +145 +00:10:45,820 --> 00:10:51,000 +معاه؟ هيصير double-stranded RNA حد مش .. مصطلح مش + +146 +00:10:51,000 --> 00:10:55,580 +دارج هنا double-stranded RNA مش مصطلح دارج هنا هو + +147 +00:10:55,580 --> 00:10:59,870 +عبارة عن single strandهذا ال .. ال .. ال + +148 +00:10:59,870 --> 00:11:04,010 +complementary strand احنا بنسميه antisense لإن في + +149 +00:11:04,010 --> 00:11:07,710 +مصطلح كمان انه ال .. ال mRNA بيسموه positive sense + +150 +00:11:07,710 --> 00:11:10,790 +positive sense يعني جاهز لل .. لل .. لل .. لل + +151 +00:11:10,790 --> 00:11:14,090 +translation لإن هت .. في نفس المحاضرة اليوم هتكلم + +152 +00:11:14,090 --> 00:11:16,750 +عن الفيروسات هتكلم عن حالة اسمها ال negative sense + +153 +00:11:16,750 --> 00:11:23,230 +مادة وراتية .. مادة وراتية RNA لكن مش جاهزة انها + +154 +00:11:23,230 --> 00:11:28,230 +يصبح translation لازم تتحول ل positive senseعموما + +155 +00:11:28,230 --> 00:11:32,830 +الان صار في عندي complementary strand لل mRNA بطل + +156 +00:11:32,830 --> 00:11:37,210 +الريبوزوم قدر ان يعمله expression يعني translation + +157 +00:11:37,210 --> 00:11:42,130 +وبالتالي انا ممكن اواجه تصنية vital protein في + +158 +00:11:42,130 --> 00:11:46,220 +الخلية البكتيرية وبالتالي اموت البكتيريهذا tech + +159 +00:11:46,220 --> 00:11:49,860 +تقنية شغالة لها كويس و أنتجوا بعض المنتجات لكن + +160 +00:11:49,860 --> 00:11:53,560 +بتظهر عملية انه يعني تخيل ماناخدها oral كيف بيبقى + +161 +00:11:53,560 --> 00:11:56,840 +متص ال .. ال antisense كيف بيدخل الخلايا هذا موضوع + +162 +00:11:56,840 --> 00:12:00,560 +شوية معظم البيانات اللي بنكون بتاعتنا من الجزء + +163 +00:12:00,560 --> 00:12:03,340 +الأول الصحيح من المحادثة اليوم اللي هو ال + +164 +00:12:03,340 --> 00:12:07,760 +antimicrobials هنزلكوا بعض ال animation إضافية + +165 +00:12:07,760 --> 00:12:11,020 +كمان ان شاء الله متعلقة بهذا الموضوع + +166 +00:12:18,040 --> 00:12:23,040 +الموضوع الجديد اليوم اللي هو الفيروس هذا طبعا + +167 +00:12:23,040 --> 00:12:27,300 +انتوا عارفين انه بيكون كورس كامل اسمه Virology لكن + +168 +00:12:27,300 --> 00:12:31,800 +في essential medical microbiology لازم نغطي بعض + +169 +00:12:31,800 --> 00:12:34,860 +الجوانب المتعلقة بالفيروس و على الأقل هتكلم عن + +170 +00:12:34,860 --> 00:12:38,560 +شغلتين على ال viral structure و على ال viral + +171 +00:12:38,560 --> 00:12:42,020 +replication خلال الأسبوع هذا اللي هو اليوم نهايته + +172 +00:12:42,020 --> 00:12:44,420 +و الأسبوع القادم اللي بنني فيه ان شاء الله ال + +173 +00:12:44,420 --> 00:12:44,660 +course + +174 +00:12:50,510 --> 00:12:54,430 +ما يميز الفيروسات أنه كان فيها ليها خلاف في يوم من + +175 +00:12:54,430 --> 00:12:57,310 +الأيام، هل هي كائنات حية أو أعتقد أنهم نقلوا + +176 +00:12:57,310 --> 00:13:00,750 +الكورة في المدارس و أنا بزمي جدل عقيم، يعني الأصل + +177 +00:13:00,750 --> 00:13:04,410 +ما نجادل فيه، يعني مش دايما التصنيفات .. مش دايما + +178 +00:13:04,410 --> 00:13:07,910 +التصنيفات أنه كائن حي ولا جماد يعني مفيدة لنا، هو + +179 +00:13:07,910 --> 00:13:10,530 +يمتلك صفة من صفات الحياة اللي هي ال replication + +180 +00:13:10,530 --> 00:13:14,410 +فقط في حال وجوده داخل الخلايا، و تانية من الجثة، + +181 +00:13:14,410 --> 00:13:19,490 +هي معلومة بتميز هذه الكائنات اللي اسمها فيروسلكن + +182 +00:13:19,490 --> 00:13:23,450 +وصفناها إنها obligate intracellular parasites لهذا + +183 +00:13:23,450 --> 00:13:29,430 +السبب إنه لا تستطيع تكاتر خارج الخلية السؤال لماذا + +184 +00:13:29,430 --> 00:13:34,230 +لا تستطيع تكاتر خارج الخلية؟لا .. لا .. لا تنتلك + +185 +00:13:34,230 --> 00:13:38,050 +مقوم .. لا .. لا .. لها DNA و .. لها DNA ورنية لكن + +186 +00:13:38,050 --> 00:13:42,430 +لا تنتلك ال ribosomes لا تنتلك بظبط اللي هي ال + +187 +00:13:42,430 --> 00:13:46,690 +organelles ما بتنتلكش ال machinery اللي هي المعدات + +188 +00:13:46,690 --> 00:13:51,670 +اللي موجودة في الخلية العادية وبالتالي تضطر آسفة + +189 +00:13:51,670 --> 00:13:57,550 +إنها تتطفل على عوائل أخرى للحصول على هذه الأدوات + +190 +00:13:57,550 --> 00:14:02,960 +والمعدات اللي احنا حكينا عنهاbecause they can + +191 +00:14:02,960 --> 00:14:06,260 +reproduce only within cells, viruses are obligate + +192 +00:14:06,260 --> 00:14:09,840 +intracellular parasites هذه الصفة لم نراها في أي + +193 +00:14:09,840 --> 00:14:13,020 +بكتيريا إلا في دنتين كلاميديا وريكتسيا ممتاز + +194 +00:14:13,020 --> 00:14:16,620 +كلاميديا وريكتسيا and both of them they cannot + +195 +00:14:16,620 --> 00:14:20,080 +replicate independently خدوا بالكم فرق كبير بين + +196 +00:14:20,080 --> 00:14:23,500 +الكلاميديا وريكتسيا وما بين الفيروس الكلاميديا + +197 +00:14:23,500 --> 00:14:29,020 +وريكتسيا are بكتيريا كل المكونات موجودة ما عدا + +198 +00:14:29,020 --> 00:14:35,730 +عندها مشكلة واحدةthey cannot synthesize ATP يعني + +199 +00:14:35,730 --> 00:14:40,630 +ماهموش فلوس يشتروا اغراض من في الخلية فبالتالي او + +200 +00:14:40,630 --> 00:14:44,790 +يتبادلوا الطاقة بالخلية انتوا عارفين التبادل بيكون + +201 +00:14:44,790 --> 00:14:49,570 +بال ATP فهم بيسموهم حتى انهم اسم لطيف بيسموهم + +202 +00:14:49,570 --> 00:14:53,690 +energy parasites يعني بتتففر من أجل ال energy اللي + +203 +00:14:53,690 --> 00:14:58,070 +هو ال ATP viruses + +204 +00:14:58,070 --> 00:15:03,000 +do not replicateIn a similar way to that of cells + +205 +00:15:03,000 --> 00:15:07,520 +الخلايا سواء كانت Eukaryote او بلوكاريوت فيها انه + +206 +00:15:07,520 --> 00:15:10,140 +simple binary vision انقسام ثنائي و أخر الخلية + +207 +00:15:10,140 --> 00:15:14,020 +تصير تنتهي و في ال mitosis و ال meiosis برضه كمان + +208 +00:15:14,020 --> 00:15:17,620 +نفس الجثة فيها اندي انقسام واضح انه فيه زيادة في + +209 +00:15:17,620 --> 00:15:21,720 +عدد الخلايا الواحدة بصير تنتهيلكن الفيروسات ال + +210 +00:15:21,720 --> 00:15:25,440 +virus will enter the whole cell one single + +211 +00:15:25,440 --> 00:15:29,180 +particle و it will be released hundreds and + +212 +00:15:29,180 --> 00:15:32,260 +thousands على حسب نوع الفيروس نفسه hundreds and + +213 +00:15:32,260 --> 00:15:34,560 +thousands of particles are released from this + +214 +00:15:34,560 --> 00:15:38,300 +cell، إذا انت قامي اتكارته و اتبعته مختلفة تمام + +215 +00:15:41,450 --> 00:15:46,210 +general characteristics لل viruses ال viruses أول + +216 +00:15:46,210 --> 00:15:51,290 +هام فيها أنه يا المادة الوراثية يا اما DNA يا اما + +217 +00:15:51,290 --> 00:15:57,350 +RNA but never both اللي أعقد من هيك نوع المادة + +218 +00:15:57,350 --> 00:15:59,070 +الوراثية مش هتكلم فيها اللي احتجت لانا في لوحات + +219 +00:15:59,070 --> 00:16:02,950 +سابقةالمادة البراطينية نفسها احنا كلنا نعرف DNA + +220 +00:16:02,950 --> 00:16:07,150 +double-strand و ال RNA single-strand و بالظبط هنا + +221 +00:16:07,150 --> 00:16:10,410 +هنشوف انه فينا double-stranded DNA و single + +222 +00:16:10,410 --> 00:16:13,550 +-stranded RNA و double-stranded mRNA و single + +223 +00:16:13,550 --> 00:16:17,730 +-stranded mRNA و هنشوف في ال single-stranded + +224 +00:16:17,730 --> 00:16:23,050 +positive sense و negative sense و هنشوف لينيا او + +225 +00:16:23,050 --> 00:16:27,930 +segmented مقطع يعني بدل من جنوب يكون قطع واحدة + +226 +00:16:27,930 --> 00:16:35,740 +يكون قدت قطعبعض الفيروسات تمتلك ممبرين حول الكبسيط + +227 +00:16:35,740 --> 00:16:39,980 +تبعتها والبعض الآخر لايمتلكها فبنقول عنها envelope + +228 +00:16:39,980 --> 00:16:44,200 +و non envelope اللي إلها بسميها envelope عشان نكون + +229 +00:16:44,200 --> 00:16:46,720 +ماضحينه من البداية هذا ال envelope من أين بيجي؟ + +230 +00:16:47,120 --> 00:16:51,900 +بييجي الفيروس هو الطالع يا إما من ال nuclear + +231 +00:16:51,900 --> 00:16:56,720 +membrane يا من ال cell membrane يبقى مصدر .. مصدر + +232 +00:16:56,720 --> 00:17:00,800 +ال envelope هو من ال nuclear membrane أو من ال + +233 +00:17:00,800 --> 00:17:05,760 +cell membrane تبع ال host cell مين أجواء؟ مين + +234 +00:17:05,760 --> 00:17:09,220 +أحسن؟ اللي له envelope ولا اللي ماروش envelope؟ + +235 +00:17:09,220 --> 00:17:17,240 +حسب .. حسب .. حسب إجابة .. حسب ما يتبعها تبريرطالع + +236 +00:17:17,240 --> 00:17:24,040 +عليك اللي أخد membrane استفاد حاجة إيش الحاجة هذه؟ + +237 +00:17:24,040 --> 00:17:28,640 +إنه الآن لو دخل ل host تاني ال membrane اللي على + +238 +00:17:28,640 --> 00:17:33,940 +جدفه اللي حامله تمام ال envelope هو جزء من ال .. + +239 +00:17:33,940 --> 00:17:37,380 +ال .. ال host وبالتالي سهل جدا إنه membrane مع + +240 +00:17:37,380 --> 00:17:41,160 +membrane zero مع fusion اه fusion فبدخل الفيروس + +241 +00:17:41,160 --> 00:17:47,150 +داخل خلية بكل سهولةيبجي هى الميزة انه مابيعانيش من + +242 +00:17:47,150 --> 00:17:51,370 +موضوع ال penetration و ال attachment لكن خدوا + +243 +00:17:51,370 --> 00:17:57,230 +فالكوا مدام اركن واعتمد على ال envelope يدخله + +244 +00:17:57,230 --> 00:18:01,070 +الخلايا لو راح ال envelope هذا معناه الحكى انه مش + +245 +00:18:01,070 --> 00:18:07,650 +هاجدر يعمل infection وما اسهل ان تذهب او يذهب هذا + +246 +00:18:07,650 --> 00:18:12,510 +ال envelope ليش اتذكروا membraneفاكرين ال olive + +247 +00:18:12,510 --> 00:18:16,750 +oil؟ اللي وزي الزيتون، very fragile، لو غسلنا + +248 +00:18:16,750 --> 00:18:19,430 +إيدينا بصبون، على طول الصبون بيعمل إيه؟ + +249 +00:18:19,430 --> 00:18:23,590 +Destruction للممبرين، لو جاف الممبرين، الفيروس + +250 +00:18:23,590 --> 00:18:27,790 +يعني جافي، الممبرين راحوا، لو بلعناها في الأمعاء، + +251 +00:18:27,790 --> 00:18:32,690 +في ال .. في ال .. في الصمت، هيتدمريبجى معنى الحكي + +252 +00:18:32,690 --> 00:18:37,650 +انه بعطيه نوع من الحساسية و عدم المقاومة عدم + +253 +00:18:37,650 --> 00:18:41,870 +المقاومة في ال environment لذلك لا نجد ولا + +254 +00:18:41,870 --> 00:18:44,870 +envelope virus بيعمل infection في ال + +255 +00:18:44,870 --> 00:18:48,290 +gastrointestinal tract كلهم بيعمله في الغالب + +256 +00:18:48,290 --> 00:18:51,050 +respiratory tract infection respiratory tract + +257 +00:18:51,050 --> 00:18:55,910 +infection و هشوف طبعا أمثلة عليهم لحق لكن اللي + +258 +00:18:55,910 --> 00:18:58,950 +بدنا نفهمه انه مش وجود .. موجود envelope يعني انه + +259 +00:18:58,950 --> 00:19:04,750 +حاجة يعني ..مية بالمية بتعطي الفيروس حاجة قوة او + +260 +00:19:04,750 --> 00:19:07,330 +صلابة في البيئة لأ بالعكس بتعطيه عدم ال stability + +261 +00:19:07,330 --> 00:19:14,650 +في ال environment طيب الفيروسات بتحتوي على مادة + +262 +00:19:14,650 --> 00:19:18,170 +وراثية و ان هي DNA اما ارنب لكن واقع الأمر لا + +263 +00:19:18,170 --> 00:19:24,670 +تمتلك nucleus لا تمتلك nucleus بينما ال eucariotes + +264 +00:19:24,670 --> 00:19:28,390 +كلها تمتلك nucleus طب و ال eucariotes؟ + +265 +00:19:33,920 --> 00:19:37,840 +الفروقات الأساسية واللي بوقرت ال eukaryotes هو + +266 +00:19:37,840 --> 00:19:41,800 +عشان احنا نكون واضحين هو فيه nucleus بس مش ال true + +267 +00:19:41,800 --> 00:19:45,920 +nucleus سمناها لإن مافيش فيها nuclear membrane + +268 +00:19:45,920 --> 00:19:51,440 +فسمناها .. يعني مش ال true nucleus تمام؟ بينما ال + +269 +00:19:51,440 --> 00:19:56,260 +eukaryotes لإن فيها سمناها true nucleusلكن فيها + +270 +00:19:56,260 --> 00:20:00,300 +مادة وراثية، فيها كروموزوم صح، البكتيريا، فيها كل + +271 +00:20:00,300 --> 00:20:04,360 +ال machinery، لكن مش فيها mitochondria، لكن ال .. + +272 +00:20:04,360 --> 00:20:07,060 +يعني بما أخر في فروقات بين الفيروسات و بين + +273 +00:20:07,060 --> 00:20:11,260 +البروكاريوتس و بين ال .. لكن في النهاية + +274 +00:20:11,260 --> 00:20:15,080 +البروكاريوتس أقرب إلى ال eukaryotes من مين؟ من + +275 +00:20:15,080 --> 00:20:15,900 +الفيروسات + +276 +00:20:20,350 --> 00:20:25,630 +هذا بالطبع بيلخص الأمر تماما صحيح types of nucleic + +277 +00:20:25,630 --> 00:20:30,070 +acid ال virus is DNA or RNA but never both لكن ال + +278 +00:20:30,070 --> 00:20:33,930 +cells بتكون فيها كل من ال DNA و ال RNA ال proteins + +279 +00:20:33,930 --> 00:20:38,110 +بعض ال فيروسات تحتوي + +280 +00:20:38,110 --> 00:20:42,790 +على protein منها matrix protein منها منها + +281 +00:20:42,790 --> 00:20:48,390 +glycoproteins منها ما هو enzymes تخيلوا فيروس عنده + +282 +00:20:48,390 --> 00:20:53,630 +ال genome تبعه RNAو negative sense، بده يروح على + +283 +00:20:53,630 --> 00:20:57,570 +خلية، هو مفكر، كل ما روح، بقول طب أنا روح على + +284 +00:20:57,570 --> 00:21:01,560 +الخلية، بدأ أجي عندهمبيلاقي عندهم enzymes أستفيد + +285 +00:21:01,560 --> 00:21:04,600 +منها في إن أنا أتحول من negative sense ل positive + +286 +00:21:04,600 --> 00:21:09,220 +sense الجواب لأ فبقول طب أنا بتصنع ال enzyme تبقى + +287 +00:21:09,220 --> 00:21:12,860 +هدوء أفضل معاه و أنا رايح على الخليج فبعض ال .. ال + +288 +00:21:12,860 --> 00:21:16,540 +.. ال بيروزات بتحمل معاها كما ذكرنا بعض ال enzyme + +289 +00:21:16,540 --> 00:21:21,360 +زي ال HIV virus بيحمل معاه reverse transcriptase + +290 +00:21:21,360 --> 00:21:25,980 +reverse transcriptase بينما الخلايا فيها حياتها + +291 +00:21:25,980 --> 00:21:28,340 +كلها proteins حياتها كلها ال proteins + +292 +00:21:34,490 --> 00:21:41,170 +الخلايا كلها نبات، حيوان، بكتيريا، فطريات في الـH + +293 +00:21:41,170 --> 00:21:47,040 +cellRibosomes absent لكن في إشارة إنه في معلومة + +294 +00:21:47,040 --> 00:21:50,980 +تحت بتقول إنه بعض الفيروسات اللي اسمها Arena + +295 +00:21:50,980 --> 00:21:55,840 +viruses تحتوي على non-functional ribosomes إيش هي + +296 +00:21:55,840 --> 00:22:00,200 +non-functional ribosomes؟ يعني ريبوزومات ملاش .. + +297 +00:22:00,200 --> 00:22:04,940 +لأ غير فعالة، غير نشطة، يعني حتى لو حطنا لها mRNA + +298 +00:22:04,940 --> 00:22:09,020 +مش هتشتغل، non-functional، غير عن كلمة مش معروفة + +299 +00:22:09,020 --> 00:22:09,900 +الوظيفة + +300 +00:22:12,570 --> 00:22:17,070 +لأ كلمة مالهاش وظيفة يعني إن هي شغالة بس مش كده + +301 +00:22:17,070 --> 00:22:20,230 +تعمل حاجة، بس non-functional يعني خربانة خدنا + +302 +00:22:20,230 --> 00:22:24,170 +نقول، يعني لو حطنا لها إحنا إيه معلنة إيه وكده مش + +303 +00:22:24,170 --> 00:22:24,570 +هتشتغل + +304 +00:22:28,500 --> 00:22:31,980 +قولنا ال .. ال .. ال .. ال metachondria absent + +305 +00:22:31,980 --> 00:22:35,600 +بينما في ال .. في ال eukaryotes بتكون موجودة ال + +306 +00:22:35,600 --> 00:22:39,760 +enzymes none or few يا إما مافيش يا إما طاليلة + +307 +00:22:39,760 --> 00:22:43,520 +بينما الخلايا كتير ال multiplication by binary + +308 +00:22:43,520 --> 00:22:47,920 +vision الخلايا نعم بينما اللي هو ال viruses + +309 +00:22:47,920 --> 00:22:51,960 +مابتنقسمش بالطريقة هذه و احنا قولنا انه one cell + +310 +00:22:51,960 --> 00:22:54,740 +one virus one particle و اللي مدى هو several + +311 +00:22:54,740 --> 00:23:00,010 +hundred particlesهي جيه ال size تبقى للميروسة Size + +312 +00:23:00,010 --> 00:23:05,210 +الحجم اللى احنا شفناه فى البكتيريا كنا نقوله انه + +313 +00:23:05,210 --> 00:23:09,710 +واحد مايكرون وشحته هذا الواحد مايكرون صار الف + +314 +00:23:09,710 --> 00:23:15,590 +نانومتر الألف لحظه أكبر بيروس 300 نانومتر يعنى + +315 +00:23:15,590 --> 00:23:19,250 +البكتيريا صغيرة واحد مايكرون حجمها ثلاث أضعاف حجم + +316 +00:23:19,250 --> 00:23:19,570 +اللى هو + +317 +00:23:23,170 --> 00:23:28,890 +والبكتيريا الصغيرة خالص قد تصل بعض البروتينات في + +318 +00:23:28,890 --> 00:23:34,590 +حجمها الى 20 نانومتر وقد تصل بعض البكتيريا في + +319 +00:23:34,590 --> 00:23:39,410 +صغرها الى 300 نانومتر تشوف الحجم هنا range واسع + +320 +00:23:39,410 --> 00:23:47,070 +اكبر بروتين 20 نانومترتلات ميت نازنوميتا مين أزار + +321 +00:23:47,070 --> 00:23:51,730 +البكتيريا؟ أظن ال micro plasma دي كمان بكتيريا + +322 +00:23:51,730 --> 00:23:53,810 +اسمها كلاميديا بس كنا بتكلم عنها قبل شوية + +323 +00:23:53,810 --> 00:23:58,190 +الكلاميديا في إليها طورين تختلف عن باقي أنواع + +324 +00:23:58,190 --> 00:24:01,470 +البكتيريا اللي في الدنيا بإن فيها stages مين درسته + +325 +00:24:01,470 --> 00:24:07,250 +فيلياد؟ طفلياد طب بتعرفوا حاجة اسمها ال cyst الطور + +326 +00:24:07,250 --> 00:24:11,590 +المتحوصل و Trophocyte اللي هو الطور المتغذى الخضري + +327 +00:24:11,590 --> 00:24:16,980 +هذه مرحلتينالخلية ممكن تتحول من Etrophosite إلى + +328 +00:24:16,980 --> 00:24:22,720 +Cyst ال Cyst أكتر مقاوم من مين؟ من Etrophosite طب + +329 +00:24:22,720 --> 00:24:25,380 +هذا الكلام في البكتيريا ماشيفنهوش مافيش life cycle + +330 +00:24:25,380 --> 00:24:29,220 +في البكتيريا أساسا مافيش life cycle إلا في + +331 +00:24:29,220 --> 00:24:34,940 +البكتيريا هذه فيها life cycle تتحول منreticulate + +332 +00:24:34,940 --> 00:24:40,520 +body إلى ما يشبه ال sports إلى ما يشبه ال sport + +333 +00:24:40,520 --> 00:24:44,100 +هنشوفها كمان شوية ففيها نوع من ال life cycle خدنا + +334 +00:24:44,100 --> 00:24:48,260 +نقول وين كانت ال life cycle بسيطة إذا ال size + +335 +00:24:48,260 --> 00:24:52,180 +بتروح من 20 نانومتر إلى 300 نانومتر وهذا الكلام لن + +336 +00:24:52,180 --> 00:24:55,480 +نستطيع بأي حالة الأحوال رؤيته بوضوح تحت ال + +337 +00:24:55,480 --> 00:24:59,680 +microscope العامإذا البكتيريا الرواية اللي طولها 2 + +338 +00:24:59,680 --> 00:25:04,000 +مايكرون و 3 مايكرون على قوة تكبير ألف بنشوفها شحتة + +339 +00:25:04,000 --> 00:25:07,040 +حمرة صغيرة بدون أي تفاصيل من هناك نشوف الفلاجلة + +340 +00:25:07,040 --> 00:25:13,020 +تبعها فباكل بيروسات بدنا electron microscope their + +341 +00:25:13,020 --> 00:25:16,580 +shapes are frequently referred to in colloquial + +342 +00:25:16,580 --> 00:25:22,990 +terms يعنيمصطلحات تشبيهية خلينا نسميها مثلا بنقول + +343 +00:25:22,990 --> 00:25:27,250 +شكله زي الكورة، شكله زي القضيب يعني أو العصي أو ال + +344 +00:25:27,250 --> 00:25:31,150 +.. ال .. ال .. البسلاي أو ال bullet، رصاصة مثلا + +345 +00:25:31,150 --> 00:25:35,830 +تمام؟ يعني بيعطيهم أوصاف للشكل الخارجي كما تبدو + +346 +00:25:35,830 --> 00:25:38,650 +تحت ال electron microscope زي الأخيرة ال bricks + +347 +00:25:38,650 --> 00:25:44,470 +اللي هو الطوب، ال block يعني but in reality احنا + +348 +00:25:44,470 --> 00:25:49,070 +بيعطيها للأوصاف بناء على أيش قلنا؟ على شيء خارجلكن + +349 +00:25:49,070 --> 00:25:54,170 +in reality they are complex in structures and they + +350 +00:25:54,170 --> 00:25:58,190 +usually have symmetry، ايش هي symmetry؟ تماثل، هذا + +351 +00:25:58,190 --> 00:26:04,710 +تماثل دقيق، هندسيا دقيق، geometric symmetry وكلمة + +352 +00:26:04,710 --> 00:26:11,790 +precise معناها تماثل هندسي دقيقأجمل ما فى التماثل + +353 +00:26:11,790 --> 00:26:16,310 +الدقيق هنا هو المكون .. بحاجات اللى بنشوفه من + +354 +00:26:16,310 --> 00:26:21,150 +الشكل الخارجى، احنا بنشوف ال capsule المكونة من + +355 +00:26:21,150 --> 00:26:25,510 +مين؟ من Capsules Capsules هى الوحدات البنائية + +356 +00:26:25,510 --> 00:26:30,590 +تخيلوا معايا التالية، معايا كبشة بانانير تابل جلول + +357 +00:26:30,590 --> 00:26:34,950 +أكيد أخبتكوا الموسم هو ده موسم .. ماسكتهم أنا هيك + +358 +00:26:34,950 --> 00:26:40,900 +و رميتهم على الحارةلما اصدروا بالحاط وجهه بدأوا + +359 +00:26:40,900 --> 00:26:43,900 +يترتبوا لحالهم تلقائيا واحد يطلع في وجه التاني و + +360 +00:26:43,900 --> 00:26:47,400 +في وجه التاني و احنا بشكلي .. بعملش شكل هندسي معين + +361 +00:26:47,400 --> 00:26:52,880 +ايش ميزة .. ايش الميزة اللي انا بحصدها او هالجلول + +362 +00:26:52,880 --> 00:26:58,130 +كلهم حجم واحد صح؟كلهم من نوع واحد، المصنع واحد + +363 +00:26:58,130 --> 00:27:01,730 +وبالتالي بيلزمنيش اكتر من gene، بدي gene واحد فقط + +364 +00:27:01,730 --> 00:27:06,510 +لغاية، gene واحد، يبقى القبق على ال chromosome خف + +365 +00:27:06,510 --> 00:27:11,430 +ولا زاد، خف لما gene واحد مطلوب، هدأ انهم يكونوا + +366 +00:27:11,430 --> 00:27:17,910 +نوع واحد، حجم واحد، طب انهم مترتبوا لحالهم؟بيعفونى + +367 +00:27:17,910 --> 00:27:22,150 +من مسألة ان انا هصنع انزايم تمسك الحقب وتحطها فى + +368 +00:27:22,150 --> 00:27:25,730 +الحقب وتلزجها مظبوط او لأ فبدل ان هم بيعملوا self + +369 +00:27:25,730 --> 00:27:29,430 +assembly يبقى كمان بيوفروا عليا energy بيوفروا + +370 +00:27:29,430 --> 00:27:32,490 +عليا كمان برضه ال genome يعنى مافيش داعى ان انا + +371 +00:27:32,490 --> 00:27:36,230 +اصنع لهم انزيمات ترتبهم مع بعض وال CAPSOMERES ثبت + +372 +00:27:36,230 --> 00:27:39,990 +علميا انهم لما حطناها فى test tube عملت self + +373 +00:27:39,990 --> 00:27:46,700 +assembly تجميع تلقائى بدون اىتدخل أو أي إنزيمات + +374 +00:27:46,700 --> 00:27:51,000 +وكأنك بتتكلم عن science fiction يعني عن .. عن خيال + +375 +00:27:51,000 --> 00:27:54,820 +علمى أن هذه الجزئيات بترتب لحدها و تكوّنها هذا + +376 +00:27:54,820 --> 00:27:59,560 +الشكل اللي سمناها precise geometric symmetry على + +377 +00:27:59,560 --> 00:28:05,260 +الرغم من صغرها بساطتها أه إلا أنها بتحمل تعقيدات + +378 +00:28:05,260 --> 00:28:08,680 +كبيرة ال repeating subunits اللي هحكينا عنهم اللي + +379 +00:28:08,680 --> 00:28:13,280 +هم الكابسيومين هم اللي بيكونوا الكابسيتعالوا + +380 +00:28:13,280 --> 00:28:16,700 +تشوفوا السفينة في الفضاء هذه الحمراء، هذه الكورة + +381 +00:28:16,700 --> 00:28:20,740 +الدم حمراء، وهذه البكتيريا اللي هي Titanic، + +382 +00:28:20,740 --> 00:28:24,380 +همناها؟ هذه هي إيش؟ السفينة .. السفينة الضخمة اللي + +383 +00:28:24,380 --> 00:28:29,020 +شايفينها، هذه ال eco line، بينما البكتيريا هذه بتش + +384 +00:28:29,020 --> 00:28:32,020 +.. أو الفيروس، هذا بيشبه ال bullet shape + +385 +00:28:35,880 --> 00:28:39,860 +اللي هو طوب تمام ال block و هذا ال bacteriophage + +386 +00:28:39,860 --> 00:28:43,400 +الشهير عارفين من ال bacteriophage تمام؟ و شوفوا + +387 +00:28:43,400 --> 00:28:47,880 +بعض الفيروسات الصغيرة البعض طويل و رفية البعض طويل + +388 +00:28:47,880 --> 00:28:52,120 +و ضخم و هذا هو اللي مجلج الدنيا هذه الأيام اللي + +389 +00:28:52,120 --> 00:28:56,440 +اسمه Ebola virus اللي هو بسموه worm-like worm-like + +390 +00:28:56,440 --> 00:29:00,400 +يعني زي الدودة عامل زي شكل الدودة لكن لو جربنا + +391 +00:29:00,400 --> 00:29:04,880 +أكتراكتر اكتر اكتر هنشوف تفاصيل دقيقة رائعة جدا + +392 +00:29:04,880 --> 00:29:09,500 +وعملية ترتيب لل repeating subunits اللي سميناها + +393 +00:29:09,500 --> 00:29:14,780 +capsules بطريقة واحدة من تنتيجة يا اما helical + +394 +00:29:14,780 --> 00:29:20,500 +حلزونية يا اما eicosahedral ايش يعني eicosahedral + +395 +00:29:28,260 --> 00:29:32,020 +عشروني الوجوه عشروني الوجوه هنشوف صور كمان شويه + +396 +00:29:32,020 --> 00:29:37,220 +أدق لكن خلينا نتأمل شويه في الحجوم على اللوحة + +397 +00:29:37,220 --> 00:29:41,920 +اللوحة بتعطينا إيهاء بالحجوم تبعت الفيروسات + +398 +00:29:41,920 --> 00:29:46,300 +وبالتالي بتعطينا فكرة إن احنا بالفعل درست و رأيتهم + +399 +00:29:46,300 --> 00:29:50,700 +بالمايكروسكوب تبتضؤي؟ سهل او مابلعفش أشوفيه كده + +400 +00:29:50,700 --> 00:29:55,970 +هيك او مابلعفش أشوفيهأه يعني احنا حبينا في الصورة + +401 +00:29:55,970 --> 00:30:01,010 +هذه نعطي إيها بالفروقات في الحجوم من أكبر فيروس زي + +402 +00:30:01,010 --> 00:30:05,210 +الابولا و ال box virus هذا بتبقى روض ضخم هذا ال + +403 +00:30:05,210 --> 00:30:09,330 +box virus هو من أضخم الفيروسات لإن هو طول بعرض مش + +404 +00:30:09,330 --> 00:30:12,310 +دي هنا بس طول هنا طول لإن هو أكتر شي ال ال + +405 +00:30:12,310 --> 00:30:17,570 +elongation هنا طويلة كتير و رافعة لكن في الآخر + +406 +00:30:17,570 --> 00:30:21,900 +كلهمكلهم كانوا يحتي بجوش أو ممكن يقعدوا على ال + +407 +00:30:21,900 --> 00:30:24,940 +cell membrane أو ال cell wall تبع الإشريش هي كولة + +408 +00:30:24,940 --> 00:30:29,640 +مؤشر على إنهم بالفعل they are small in size لكن + +409 +00:30:29,640 --> 00:30:32,520 +variable variable في ال shapes و variable في ال + +410 +00:30:32,520 --> 00:30:37,020 +size طيب السؤال التانيالصورة التانية تمثيل بسيط ل + +411 +00:30:37,020 --> 00:30:40,540 +two viruses وراينا ال core و ال core اللي جوا دهاش + +412 +00:30:40,540 --> 00:30:44,880 +ديكون ديكون هنا يا إما DNA يا إما RNA و هذا بيسميه + +413 +00:30:44,880 --> 00:30:48,540 +احنا كله collectively نوكليو كابسيد نوكليو كابسيد + +414 +00:30:48,540 --> 00:30:52,160 +مع نوكليو كابسيد بيسميه نوكليو كابسيد فأبسط فيروس + +415 +00:30:52,160 --> 00:30:55,760 +بتكون من نوكليو كابسيد بعض الفيروسات يضاف إليها + +416 +00:30:55,760 --> 00:30:59,380 +اللي قلنا عنه ال envelope تمام فبنسميها envelope + +417 +00:30:59,380 --> 00:31:02,100 +viruses زي اللي قدامنا هاي ال envelope viruses + +418 +00:31:02,100 --> 00:31:06,280 +اللحظة طالع منها أشواك بسميها spikesهذه ال spikes + +419 +00:31:06,280 --> 00:31:10,760 +مكوّنة من إشلايكوبروتينز إشلايكوبروتينز و + +420 +00:31:10,760 --> 00:31:15,600 +بيختصروها GP GP مهم جدا ال GP هذا جاء في الامتحان + +421 +00:31:15,600 --> 00:31:18,700 +عشان تكتبوه جاء في الامتحان ال GP أو ال + +422 +00:31:18,700 --> 00:31:21,180 +إشلايكوبروتينز لإنه في الآخر لما تدرسوا فيروسات ان + +423 +00:31:21,180 --> 00:31:25,120 +شاء الله هتعرفوا إن ال GP له دور مهم جدا جدا جدا + +424 +00:31:25,120 --> 00:31:28,460 +في عمل ال attachment to the cells و أول خطوة من ال + +425 +00:31:28,460 --> 00:31:31,200 +viral infection هي ال attachment بدونها مافيش هنا + +426 +00:31:31,200 --> 00:31:36,480 +إمكانية ال attachmentكمان صورة بتعطينا أشكال + +427 +00:31:36,480 --> 00:31:43,880 +مختلفة ومتنومية طلعوا هنا very + +428 +00:31:43,880 --> 00:31:49,100 +very small the smallest وهذا اللي قلنا عنه ال box + +429 +00:31:49,100 --> 00:31:52,540 +virus ال dollarist هاي عشرون الوجوه شايفينه هذا + +430 +00:31:52,540 --> 00:31:57,360 +اللي هو عبارة عن مثلثات لكن في الآخر المثلثات هذه + +431 +00:31:57,360 --> 00:32:03,330 +بتعطيني شكل spherical شكل sphericalهذا طبعا بيكون + +432 +00:32:03,330 --> 00:32:06,450 +ايه؟ Spiral يبقى ايه؟ يا إما Spiral يا إما + +433 +00:32:06,450 --> 00:32:10,570 +الكوزاهيدرال في حالة تالتة اللي هي Complex ال + +434 +00:32:10,570 --> 00:32:15,690 +Complex غالبا بيكون مزيج بين ال .. ال .. ال + +435 +00:32:15,690 --> 00:32:21,110 +الكوزاهيدرال زي مثلا ال bacteriophage هذا + +436 +00:32:21,110 --> 00:32:22,330 +ال bacteriophage شايفين هو؟ + +437 +00:32:28,080 --> 00:32:31,840 +هذا الشيء بتبعته هي عبارة عن helical أو اسمه + +438 +00:32:31,840 --> 00:32:37,000 +complex أو أي شيء ليه انطبق عليه ايكوزا هيدرال او + +439 +00:32:37,000 --> 00:32:41,560 +helical بنسميه برضه complex إذا complex helical + +440 +00:32:41,560 --> 00:32:43,000 +ايكوزا هيدرال + +441 +00:32:50,630 --> 00:32:54,790 +اليه الجنون is located internally and can be + +442 +00:32:54,790 --> 00:32:59,890 +either واحد single or double-stranded DNA or + +443 +00:32:59,890 --> 00:33:05,110 +single or double-stranded RNA يبجي أكمنه عن أنها + +444 +00:33:05,110 --> 00:33:09,910 +دورة أربعة أربعة بدون أن نتكلم عن تفاصيل داخلية + +445 +00:33:09,910 --> 00:33:13,050 +بوستفسترفر double-stranded single-stranded DNA + +446 +00:33:18,300 --> 00:33:21,120 +في ال R&A قولنا لكوا في single .. في ال single + +447 +00:33:21,120 --> 00:33:25,380 +stranded في عندي positive strand أو positive sense + +448 +00:33:25,380 --> 00:33:26,460 +و negative sense + +449 +00:33:29,960 --> 00:33:32,580 +Have genetic materials composed of a single + +450 +00:33:32,580 --> 00:33:35,640 +-stranded DNA or of a single-stranded or double + +451 +00:33:35,640 --> 00:33:40,180 +-stranded RNA؟ هل درستم مخلوقات المادة الوراثية + +452 +00:33:40,180 --> 00:33:43,940 +تبعتها تتكون من single-stranded DNA قبل هيك؟ هل + +453 +00:33:43,940 --> 00:33:48,340 +درستم إن مخلوقات فيها المادة الوراثية هي single أو + +454 +00:33:48,340 --> 00:33:53,160 +double-stranded RNA فقط الفيروسة؟ إذن اليوم + +455 +00:33:53,160 --> 00:33:56,940 +هنتعرفنا على مجموعة من المخلوقات تمتلك unique + +456 +00:33:56,940 --> 00:34:01,670 +characters صفرات مميزة أو مميزة إلاThe nucleic + +457 +00:34:01,670 --> 00:34:05,630 +acids can be either linear or circular يمكن أن + +458 +00:34:05,630 --> 00:34:13,370 +تكون ممتدة أو دائمة ال DNA هو دائما مجموعة واحدة + +459 +00:34:13,370 --> 00:34:17,750 +ال DNA غالبا يكون جزء واحد بينما ال RNA يمكن أن + +460 +00:34:17,750 --> 00:34:22,490 +تكون مجموعة واحدة أو في مجموعات مختلفة الجملة اللي + +461 +00:34:22,490 --> 00:34:27,460 +بعدها بتوضح الجملة اللي قبلها مثلا بقولناboth + +462 +00:34:27,460 --> 00:34:32,240 +influenza اللي طبعا رفيقة الدولة تبعنا منذ الصغر + +463 +00:34:32,240 --> 00:34:37,140 +وهذا أحد التفسيرات اللي الآن نتكلمه لماذا ال + +464 +00:34:37,140 --> 00:34:42,060 +influenza قادرة على تغيير الاندرجينات تبعتها بشكل + +465 +00:34:42,060 --> 00:34:49,760 +مستمر لأن الجينوب تبعها segmented مقطع بدل ما هو + +466 +00:34:49,760 --> 00:34:55,260 +قطعة طويلة بيكون مقطع هذا التقطيع إيش بيعمل بيسهل + +467 +00:34:55,260 --> 00:34:59,680 +عملية التبادلتخيلوا لما واحد اتبادل كروموزوم طويل + +468 +00:34:59,680 --> 00:35:03,520 +تمام مع بيروز تاني او قطعة من بيروز .. من .. من + +469 +00:35:03,520 --> 00:35:06,220 +بيروز .. من .. من جينوم اعتقد القطعة بتكون ايه؟ + +470 +00:35:06,220 --> 00:35:10,180 +أسس خاصة ده راحنا ان في عندها تمان قطع ال + +471 +00:35:10,180 --> 00:35:14,460 +influenza عندها تمان قطع لو اتنين influenza غرب عن + +472 +00:35:14,460 --> 00:35:19,200 +بعض غرب يعني different strands دخلوا خلية واحدة + +473 +00:35:19,200 --> 00:35:19,920 +مرة واحدة + +474 +00:35:23,230 --> 00:35:27,590 +عملية ال replication أو تصنيع المادة الوراطية + +475 +00:35:27,590 --> 00:35:32,250 +بتبقى حاجة بيسموها packaging تعبية تكوّر الكبسيد + +476 +00:35:32,250 --> 00:35:35,750 +بنعبي فيه المادة الوراطية خلال عملية التعبية بيصير + +477 +00:35:35,750 --> 00:35:41,010 +في exchange تبادل ففيروس X بياخد من Y قطعة و لو + +478 +00:35:41,010 --> 00:35:45,530 +قطعة واحدة و فيروس Y بياخد من X قطعة و طلعنا new + +479 +00:35:45,530 --> 00:35:51,140 +viruses قد تكون ال new viruses بتموتلكن كل فترة و + +480 +00:35:51,140 --> 00:35:53,560 +فترة بطلعنا سورية ناجحة .. ناجحة من وجود نظرها + +481 +00:35:53,560 --> 00:35:56,540 +مهمة .. اه .. حتى ناجحة من وجود نظرها مين؟ اللي + +482 +00:35:56,540 --> 00:36:01,760 +بتموت .. و بتعملنا مشكلة اكبيرة في كوكب الأرض زي + +483 +00:36:01,760 --> 00:36:04,680 +ال influenza الطيور و ال influenza الخاتير و ما + +484 +00:36:04,680 --> 00:36:08,660 +شابه دالك .. خدوا بالكم تاريخ ال influenza شهير .. + +485 +00:36:08,660 --> 00:36:14,720 +يعني في 1918 قتلت ال influenza الإسبانية اربعين + +486 +00:36:14,720 --> 00:36:18,180 +مليون إنسان و بجوا زمان عدد السكان .. مش ده اليوم + +487 +00:36:18,680 --> 00:36:22,360 +الاربعين مليون حالة كبيرة الاربعين مليون هلولة هم + +488 +00:36:22,360 --> 00:36:25,520 +اكبر من عدد من قتل في الحرب العالمية الأولى + +489 +00:36:25,520 --> 00:36:28,240 +والثانية و الحرب الفيتنامية و الحرب الأمريكية + +490 +00:36:28,240 --> 00:36:32,820 +الأهلية ايش رايكم؟ عشانك لما كانوا الناس خايفين + +491 +00:36:32,820 --> 00:36:36,720 +لما قادت influenza الطيور كان في مبرر طبعا البعض + +492 +00:36:36,720 --> 00:36:41,860 +بيميل الى نظرية المؤامرة يقول انه شركات الأدوية و + +493 +00:36:41,860 --> 00:36:47,750 +سياسة انه بيميله الناس في ال ..بال .. بهذا الأخبار + +494 +00:36:47,750 --> 00:36:54,090 +لأ احنا بنقول انه لأول مرة البشر يمتلكوا فرصة انهم + +495 +00:36:54,090 --> 00:36:58,990 +يستعدوا لوباء قادم يستعدوا .. هذه الفرصة الوحيدة + +496 +00:36:58,990 --> 00:37:01,230 +اللي على مر التاريخ ان احنا نستعد لهذا الوباء + +497 +00:37:01,230 --> 00:37:08,310 +لأنها ترصد جيد و .. و المشكلة انه لما ال .. ال .. + +498 +00:37:08,310 --> 00:37:14,590 +الإعلامنشر المعلومات الناس صار عندها خوف كبير و + +499 +00:37:14,590 --> 00:37:17,870 +بعد ما الموجة راحت صاروا يقولوا يبقى هو مش .. مش + +500 +00:37:17,870 --> 00:37:21,290 +صحيح يبقى كذبين يبقى مش صادقين يبقى بعطونا + +501 +00:37:21,290 --> 00:37:27,010 +بيخوفونا شوفوا ال .. الفكرة كانت ويه انفلوانزة + +502 +00:37:27,010 --> 00:37:30,170 +الطيور .. انفلوانزة الطيور بيسموها highly + +503 +00:37:30,170 --> 00:37:34,590 +pathogenic strain ايش يعني highly pathogenic يعني + +504 +00:37:34,590 --> 00:37:37,980 +شدة الإمراض طبيعتها عالي جدانسبة الوفيات بيبقى + +505 +00:37:37,980 --> 00:37:40,660 +فيها تلات ستين في المية تلات ستين في المية يعني لو + +506 +00:37:40,660 --> 00:37:45,180 +ألف أصيبه تلات ستمية و تلاتين واحد بيدهم موته + +507 +00:37:45,180 --> 00:37:50,820 +تمام؟ إيش الميزة سبعتها؟ الميزة إنه مابتنتقلش من + +508 +00:37:50,820 --> 00:37:54,460 +إنسان لإنسان لازم من الطير لمين ده الإنسان + +509 +00:37:54,460 --> 00:37:59,640 +وبالتالي عدد لحوالي ست سبعة سنة ست سنات بتلفلف في + +510 +00:37:59,640 --> 00:38:05,300 +الدنيا عدد من أصيبه لإنسان زي وزي الألفانلا تجاوزه + +511 +00:38:05,300 --> 00:38:07,560 +ولا الفان تتعالوا على الجهة التانية لروح على ال + +512 +00:38:07,560 --> 00:38:12,120 +influenza الخنازير ال influenza الخنازير أصبحت + +513 +00:38:12,120 --> 00:38:14,360 +أكتر من ال influenza الموسمية وصنفت عليها ال + +514 +00:38:14,360 --> 00:38:20,360 +influenza موسمية نسبة الوفيات فيها point 005 يعني + +515 +00:38:20,360 --> 00:38:24,900 +أقل من ال influenza الموسمية نفسها يعني من المليون + +516 +00:38:24,900 --> 00:38:28,760 +بموتله لكن خدوا بالكم ان شاء الله لو التقى + +517 +00:38:28,760 --> 00:38:35,150 +الخنازير مع الطيور وتبادلواهذا أعطل هذا المفضل على + +518 +00:38:35,150 --> 00:38:40,250 +ال human transmission واحتفظ بالـhighly pathogenic + +519 +00:38:40,250 --> 00:38:45,470 +اللي 63% القتل معناه اللي حاكيه حيصير فيه سهولة + +520 +00:38:45,470 --> 00:38:48,990 +الانتقال وحيصير فيه قتل تخيلوا لما يصابوا + +521 +00:38:48,990 --> 00:38:52,950 +المليارات من البشر بال influence الطيور المحسنة + +522 +00:38:52,950 --> 00:38:53,570 +المعدلة + +523 +00:38:57,490 --> 00:39:02,370 +الموجودة ان يصير هذا الاتبادل بين الفيروسات وينتج + +524 +00:39:02,370 --> 00:39:05,770 +عنها سلالة من هذا الصندوق فلأ انا بقى ناخد + +525 +00:39:05,770 --> 00:39:09,510 +المواضيع ناخدها بمنطقية وعقلانية الفيروسات عندنا + +526 +00:39:09,510 --> 00:39:14,010 +المقدرة وكل عشر سنوات إذا بتلاحظوا إلى خمساشر سنة + +527 +00:39:14,010 --> 00:39:17,810 +بيطلع عندنا سلالات جديدة سلالات زي مثلا ال SARS + +528 +00:39:19,280 --> 00:39:22,420 +هيطلع ان اخر اشي اللي هو ال SARS او الكورونا تبعه + +529 +00:39:22,420 --> 00:39:26,140 +من ال east هذا اللي هو بالظبط السعودية جاعله ففيه + +530 +00:39:26,140 --> 00:39:29,860 +ان كل فترة و فترة بيطلع سلالات جديدة اما مقاومة لل + +531 +00:39:29,860 --> 00:39:33,680 +drugs او انها محسنة او عندها بعض الصفات اللي .. + +532 +00:39:37,730 --> 00:39:41,930 +كل الفيروسات بتحتوي على single copy كلمة single + +533 +00:39:41,930 --> 00:39:45,970 +copy إيش يعني؟ نسخة .. نسخة .. نسخة .. نسخة .. + +534 +00:39:45,970 --> 00:39:46,270 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +535 +00:39:46,270 --> 00:39:46,270 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +536 +00:39:46,270 --> 00:39:46,410 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +537 +00:39:46,410 --> 00:39:46,930 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +538 +00:39:46,930 --> 00:39:47,670 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +539 +00:39:47,670 --> 00:39:58,150 +نسخة .. نسخة .. نسخة .. نسخة .. نسخة .. + +540 +00:39:58,150 --> 00:40:01,690 +نسخة + +541 +00:40:01,690 --> 00:40:05,650 +.. نسالخلية العادية بيسميها diploid و الخلية + +542 +00:40:05,650 --> 00:40:08,970 +الجنسية بيسميها haploid اللي فيها نص عد نص عد + +543 +00:40:08,970 --> 00:40:12,390 +الكروموزومات، نص عد الكروموزومات، إيه شهر ده في عد + +544 +00:40:12,390 --> 00:40:15,290 +الكروموزومات؟ يعني من كل كروموزوم فيه two covers، + +545 +00:40:15,290 --> 00:40:19,510 +فdiploid و haploid، كل الفيروسات are haploid، + +546 +00:40:19,510 --> 00:40:25,360 +معادةال retrovirus نعم ال retro virus بيكون عبارة + +547 +00:40:25,360 --> 00:40:29,040 +عن deploy يعني فيروس ال aids اللي بينتمي له ال + +548 +00:40:29,040 --> 00:40:33,220 +retro viruses بيحتاج على two copies من الجينوم two + +549 +00:40:33,220 --> 00:40:37,980 +copies من الجينوم هاي ال exception في السطر الأخير + +550 +00:40:37,980 --> 00:40:42,460 +من اللوحة بتكلم عن the exception is the retrovirus + +551 +00:40:42,460 --> 00:40:47,060 +family whose members have two copies of their RNA + +552 +00:40:47,060 --> 00:40:52,540 +genome is that they are deployedViral capsid and + +553 +00:40:52,540 --> 00:40:55,960 +symmetry وهذه اللي انا حدثت فيها المحاضرة اليوم او + +554 +00:40:55,960 --> 00:40:59,640 +بعد الصور تبعتها The nucleic acid is surrounded by + +555 +00:40:59,640 --> 00:41:02,680 +a protein coat called a capsid وبينها ال capsid + +556 +00:41:02,680 --> 00:41:06,620 +بتكون من subunits سميناها ال capsomeres وانا + +557 +00:41:06,620 --> 00:41:09,660 +الصحيح الكلام اللي قولته على الصبور وشرحته الآن ان + +558 +00:41:09,660 --> 00:41:13,880 +كل ال capsomere بتكون من one or several proteins + +559 +00:41:13,880 --> 00:41:17,280 +can be seen only in the electron microscope as + +560 +00:41:17,280 --> 00:41:21,900 +spherical particlesSometimes with a central hole + +561 +00:41:21,900 --> 00:41:25,920 +يعني مفردة يبدو أن الفراغ هذا يعني ال protein اللي + +562 +00:41:25,920 --> 00:41:31,680 +مكوّن أو اللي هو ال hole هو اللي بيساعد ال .. ال + +563 +00:41:31,680 --> 00:41:35,580 +.. ال .. ال capsules على ما يسمى بال self assembly + +564 +00:41:35,580 --> 00:41:41,880 +اللي هو التجميع is there the structure composed of + +565 +00:41:41,880 --> 00:41:46,880 +the nucleic acid genomeand the capsid protein is + +566 +00:41:46,880 --> 00:41:49,040 +called the nucleocapsid وعن هذا الكلام حكيناه + +567 +00:41:49,040 --> 00:41:52,640 +نوكليو كابسيد قلنا عنها أنه أبسط فيروس مكوّن من + +568 +00:41:52,640 --> 00:41:56,280 +نوكليو كابسيد the arrangement of capsimers gives + +569 +00:41:56,280 --> 00:41:59,060 +the virus structure its geometric symmetry وإن + +570 +00:41:59,060 --> 00:42:07,300 +السيمتري تبعته اللي هي هذه إيكوزا هيلرالوالتاني + +571 +00:42:07,300 --> 00:42:10,620 +اللي يقول عنها helical وقلنا هي في مثلها انها لأ + +572 +00:42:10,620 --> 00:42:16,240 +هي مثلها انها اكوزا و هي naked naked يعني مالوش + +573 +00:42:16,240 --> 00:42:20,340 +envelope و envelope يبقى عكس envelope بنسميها اما + +574 +00:42:20,340 --> 00:42:25,260 +non envelope او بنسميها naked غير وانا شرحت وارجو + +575 +00:42:25,260 --> 00:42:29,000 +انه يظلوا في الذاكره ان ال envelope يلو ميزةلكن + +576 +00:42:29,000 --> 00:42:34,500 +برضه إلو عيب .. و العيب هذا يعني من .. بعدي it + +577 +00:42:34,500 --> 00:42:38,140 +confirms in أو it confirms instability of the + +578 +00:42:38,140 --> 00:42:38,860 +virus in + +579 +00:42:44,300 --> 00:42:47,740 +هذه اللوحة هي الكلام اللي حكيته عن الكبسومير، إيش + +580 +00:42:47,740 --> 00:42:50,780 +ال advantage of building the virus particle from + +581 +00:42:50,780 --> 00:42:53,700 +identical protein separates، أنا قلت ال glules، + +582 +00:42:53,700 --> 00:42:56,940 +مظبوط اللي هو identical، واحد it reduces the need + +583 +00:42:56,940 --> 00:42:59,920 +for genetic information، أعتقد كلمة genetic + +584 +00:42:59,920 --> 00:43:03,100 +information يعني مادة وراثية، يعني بدل ما نحمل .. + +585 +00:43:03,100 --> 00:43:06,400 +تخيل لو إنه مصنوعة من ..من قطع حمرا أو زرقا أو + +586 +00:43:06,400 --> 00:43:10,000 +صفرا أو أحجام مختلفة أو كده، فمعنى الحكي أنه كل + +587 +00:43:10,000 --> 00:43:13,540 +قطع بان تكون فيها جين مختلف، فهذا هيزيد من إيه؟ + +588 +00:43:13,540 --> 00:43:18,420 +فبتقلل من كفاءة ال virus It promotes self-assembly + +589 +00:43:18,420 --> 00:43:25,040 +يبقى بيحسن اللي هو أو بيساعد على التجميع التلقائي + +590 +00:43:25,040 --> 00:43:29,310 +أو ال ..الجملة .. الفقرة الأخيرة بتقول إن في + +591 +00:43:29,310 --> 00:43:36,370 +الواقع تم تكوين فيروس كامل دون وجود خلية فقط أخد + +592 +00:43:36,370 --> 00:43:38,910 +ال capsomers و حطوها في test tube و بالفعل اتكون + +593 +00:43:38,910 --> 00:43:43,410 +فيروس كامل، و عندها اللوحة ان شاء الله من المرة + +594 +00:43:43,410 --> 00:43:43,790 +القادمة + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/KvmHhaw5nZI_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/KvmHhaw5nZI_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..ce0eb6203bcade2b622f9bff2325e4d8c98bb3bd --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/KvmHhaw5nZI_raw.srt @@ -0,0 +1,2160 @@ +1 +00:00:20,640 --> 00:00:24,800 +بسم الله الرحمن الرحيم استكمالا للموضوع اللى + +2 +00:00:24,800 --> 00:00:26,420 +بدأناه المحاضرة السابقة اللى هو ال bacterial + +3 +00:00:26,420 --> 00:00:30,700 +genetics هنمره مره سريع على كل النقاط اللى مرناها + +4 +00:00:30,700 --> 00:00:34,280 +المحاضرة السابقة لكن بشكل سريع جدا تذكروا أن + +5 +00:00:34,280 --> 00:00:36,720 +المادة اللى قرار فيها بالبكتيريا هي واحدة من ثلاثة + +6 +00:00:36,720 --> 00:00:40,440 +يا إما كروموزوم يا إما بلازمت يا إما إبيزوم + +7 +00:00:40,440 --> 00:00:45,060 +والإبيزوم هو حقا بلازمت يعني يا كروموزوم يا بلازمت + +8 +00:00:45,060 --> 00:00:50,270 +في وقتهالما اتكلمنا عن ال mutation تحدثنا عن نقطة + +9 +00:00:50,270 --> 00:00:55,790 +مهمة جدا كنا انه يعني تغيير طفيف في تركيب كائن + +10 +00:00:55,790 --> 00:00:58,330 +واحد زي الموجود اللي هو حالمنا أعطانا خمس + +11 +00:00:58,330 --> 00:01:03,550 +varieties خمس أصناف اختاروا في اللون ممكن في الشكل + +12 +00:01:03,550 --> 00:01:06,110 +ممكن في المواصفات ممكن في القدر على ال survival + +13 +00:01:06,110 --> 00:01:12,150 +فالمutation أساس مهم جدا في حياة ال micro organism + +14 +00:01:12,150 --> 00:01:16,560 +لأنه single chromosome إذا تتذكرواوقلنا only one + +15 +00:01:16,560 --> 00:01:20,560 +copy of the gene فإذا صار فيه طفرة فستظهر عليها ال + +16 +00:01:20,560 --> 00:01:24,820 +phenotype سبعتها احنا ال eukaryotes لو صار طفرة + +17 +00:01:24,820 --> 00:01:27,200 +على كروموزوم على سبيل المثال على ال gene الجيش + +18 +00:01:27,200 --> 00:01:29,980 +التاني لسه مازال موجود وبتالي يمكن يغطي على تأثير + +19 +00:01:29,980 --> 00:01:33,800 +الطفرة الأولاني فظهور الطفرات في البكتيريا بيكون + +20 +00:01:33,800 --> 00:01:38,760 +أسرع قلنا عن ال genetic variation في البكتيريا + +21 +00:01:38,760 --> 00:01:42,060 +وقلنا إنه ال genetic variation تنشأ عن إما + +22 +00:01:42,060 --> 00:01:45,610 +mutationو ذكرنا ال mutation كنا فيه منها صنفين + +23 +00:01:45,610 --> 00:01:49,890 +spontaneous و induced و اتكلمنا عن ال gene + +24 +00:01:49,890 --> 00:01:52,130 +transfer و بدأنا في الحديث عن ال transformation + +25 +00:01:52,130 --> 00:01:56,030 +وصفناه بأنه ال free take up of DNA او uptake لل + +26 +00:01:56,030 --> 00:01:59,530 +DNA من ال surroundings ال conjugation هو الاقتران + +27 +00:01:59,530 --> 00:02:02,550 +الجنسي عبر البلاي و ال transduction درسناه من + +28 +00:02:02,550 --> 00:02:06,010 +انواعين اللي هو ال generalized و ال specialized + +29 +00:02:06,010 --> 00:02:09,750 +حنمره بشوية تفاصيل كلمنا عن مصطلح اسمه lysogenic + +30 +00:02:09,750 --> 00:02:12,970 +conversion البكتيريا صار جواها profage + +31 +00:02:13,980 --> 00:02:16,640 +البكتيريوفاجي اللى جوا سمنها Profagia البكتيرى + +32 +00:02:16,640 --> 00:02:20,080 +اللى حاملة ال Profagia سمنها Lysogenized cell او + +33 +00:02:20,080 --> 00:02:24,020 +Lysogenized bacteria واخر وقت اتكلمنا عنها فى ال + +34 +00:02:24,020 --> 00:02:26,760 +gene فى ال genetic variation اللى ال Protoplast + +35 +00:02:26,760 --> 00:02:32,480 +fusion wherein you unite two cells into one single + +36 +00:02:32,480 --> 00:02:36,280 +cell اكيد هيحصل فى ال combination من ال genome تبع + +37 +00:02:36,280 --> 00:02:41,700 +الخلية الأولى و ال genome تبع الخلية الثانيةوبدأنا + +38 +00:02:41,700 --> 00:02:44,440 +بالحوار أو بحوارنا المرة الماضية عن ال mutation + +39 +00:02:44,440 --> 00:02:48,140 +وكنا برضه هي صنفان نوعا ما اللي هي ال base + +40 +00:02:48,140 --> 00:02:50,820 +substitution و ال frame shift و ال frame shift + +41 +00:02:50,820 --> 00:02:55,740 +نوعا ما إما insertion أو deletion لكن في المحصلة + +42 +00:02:55,740 --> 00:02:59,580 +النهائية ال mutation هي التغيير في ال sequence ايش + +43 +00:02:59,580 --> 00:03:04,140 +هي ال sequence؟ التعبر أو ال structure تبع اللي هو + +44 +00:03:04,140 --> 00:03:05,740 +المادة الوراثة + +45 +00:03:08,000 --> 00:03:11,200 +base substitution أو frame shift وللـ frame shift + +46 +00:03:11,200 --> 00:03:15,160 +قلنا insertion أو deletion اللوحة اللي ما اتكلمنا + +47 +00:03:15,160 --> 00:03:21,500 +عنها اللي هي ال mutagenes ال مواد المطفرة المنتجة + +48 +00:03:21,500 --> 00:03:25,960 +للطفرات طبعا + +49 +00:03:25,960 --> 00:03:29,340 +اللوحة اللي قدامكوا هي نموذج مثال بسيط مثال بسيط + +50 +00:03:29,340 --> 00:03:36,540 +على مركبات أو مواد قد تسبب طفرة وسميناها ميوتاجين + +51 +00:03:37,480 --> 00:03:40,480 +chemicals زي نيتروز أسد وهو يتبع لمجموعة اسمها + +52 +00:03:40,480 --> 00:03:44,680 +alkalating agent حيمور ذكرها معانا لاحقا five + +53 +00:03:44,680 --> 00:03:49,080 +bromioracil five bromioracil هو structural analog + +54 +00:03:49,080 --> 00:03:52,720 +وبدأ أشرح إيش يعني structural analog لإنه هذا + +55 +00:03:52,720 --> 00:03:57,060 +المفهوم كتير مهم بالنسبة إلنا في مجالات متنوعة في + +56 +00:03:57,060 --> 00:04:02,600 +ال micro biome لو عندي أنا توقم طفلين توقم تمام + +57 +00:04:02,600 --> 00:04:08,740 +identical أنا مش جادر أميز بينهمواحد منهم بنعطيه + +58 +00:04:08,740 --> 00:04:15,660 +مبلغ معين من المال، تمام؟ فبيصرفوه على المنزل، + +59 +00:04:15,660 --> 00:04:20,680 +بدبّر فيه، بشتري فيه أكل، ملابس، إلى أخرى، التاني + +60 +00:04:20,680 --> 00:04:23,880 +لو أعطانا نفس المبلغ اللي ليش بعمل فيه، بحطه وضبه + +61 +00:04:23,880 --> 00:04:29,230 +في جيبته، ماحدش بيستفيد منهالـ original الطفل + +62 +00:04:29,230 --> 00:04:32,930 +الأول هو الإنزايم على سبيل المثال أو المركّب + +63 +00:04:32,930 --> 00:04:36,470 +المفيد في الخلية، الـ analog له اللي هو الشبيه + +64 +00:04:36,470 --> 00:04:40,490 +تبعه، اللي غير مفيد، ف structural analog، + +65 +00:04:40,490 --> 00:04:46,650 +structural analog، الآن هذا المفهوم تخيله كالتالي، + +66 +00:04:46,650 --> 00:04:50,600 +عندي إنزيم بيشتغل على substrate،لما .. لما ال + +67 +00:04:50,600 --> 00:04:54,020 +substrate الحقيقي بيكون موجود بيكسره ل products، + +68 +00:04:54,020 --> 00:04:57,060 +ال products هذه بتكسر ل products أخرى و بتفيد + +69 +00:04:57,060 --> 00:05:00,740 +الخلية، لكن لو ال substrate التاني اللي هو ال + +70 +00:05:00,740 --> 00:05:02,160 +analog ارتبط بالإنزيم + +71 +00:05:14,920 --> 00:05:19,200 +فقد نقتل الخلية السرطانية بنفس القالية بنحط + +72 +00:05:19,200 --> 00:05:23,340 +structural analog قد نقتل البكتيريا بنحط + +73 +00:05:23,340 --> 00:05:30,580 +structural analog قد نقتل نحن ب structural analog + +74 +00:05:32,280 --> 00:05:35,160 +الـ Phi-Promo-Uracil Structure Analog لـ Natural + +75 +00:05:35,160 --> 00:05:38,520 +Genes Basis في الـ في الـ DNA فالـ DNA Polymerase + +76 +00:05:38,520 --> 00:05:42,380 +بدل ما يحط Base معينة بيحط مين بدلها الـ Phi-Promo + +77 +00:05:42,380 --> 00:05:47,100 +-Uracil الـ Phi-Promo-Uracil مش Base حقيقية Analog + +78 +00:05:47,100 --> 00:05:51,680 +فلما بيجي بيكمل الـ DNA بقدرش يربط القاعد الأخرى + +79 +00:05:51,680 --> 00:05:56,060 +وراها يبقى وجفنا تصنيع الـ DNA لما صار عندي + +80 +00:05:56,060 --> 00:05:57,720 +mutation لما ماصارش عندي mutation صار عندي + +81 +00:05:57,720 --> 00:05:58,240 +mutation + +82 +00:06:03,080 --> 00:06:07,800 +لكن عندي chemicals لو أنا بدأ أقعد أعمل قائمة فيها + +83 +00:06:07,800 --> 00:06:11,960 +يمكن أقعد ساعة كاملة أعمل listing فقط لأسماء + +84 +00:06:11,960 --> 00:06:16,840 +المُركّبات اللي ممكن تعمل ال mutation تسمعوا في + +85 +00:06:16,840 --> 00:06:20,300 +المستشفيات إن الست الحامل هنا تدخل على غرف الأشعة + +86 +00:06:20,300 --> 00:06:25,320 +وإن اللي بيصوره في الأشعة بيلبس ست رواقية مكونة من + +87 +00:06:25,320 --> 00:06:31,640 +الرصاص يدراه أن الغرف بتكون مبطنة بالرصاصلماذا؟ + +88 +00:06:31,640 --> 00:06:35,920 +لأن الـ X-ray can cause mutation ممنوع تتعرض له + +89 +00:06:35,920 --> 00:06:40,400 +الـ UV في المختبر، في الميكروسكوبات أو في اللمبات + +90 +00:06:40,400 --> 00:06:46,910 +تبع التعقيم، بنحط الحادث shield plasticأو شيء معتم + +91 +00:06:46,910 --> 00:06:51,370 +و بيقول ممنوع ان ال UV تصيب العين او تجرب على + +92 +00:06:51,370 --> 00:06:54,370 +الجلد لانه بتعمل mutation هتكلم لحظة احنا كيف تعمل + +93 +00:06:54,370 --> 00:06:58,610 +ال mutation لكن هاي x-ray او ionizing و non + +94 +00:06:58,610 --> 00:07:01,710 +-ionizing radiation تمام عملوا mutation و + +95 +00:07:01,710 --> 00:07:05,850 +chemicals عملوا mutation النقطة الأخيرة ال viruses + +96 +00:07:05,850 --> 00:07:10,790 +هناك مجموعة من الفيروسات تسمى onco viruses onco + +97 +00:07:10,790 --> 00:07:16,090 +viruses وهي فيروسات تسبب mutation في الانسانهو حتى + +98 +00:07:16,090 --> 00:07:20,650 +في الحيوان طبعا يعني انا كلمة مرة الان هذه مقدمة + +99 +00:07:20,650 --> 00:07:24,830 +لنا احنا نعرف انه فيه مركبات او عوامل بتسبب + +100 +00:07:24,830 --> 00:07:28,830 +المتايشير لكن هذا .. هذه ليست قائمة حصرية و انما + +101 +00:07:28,830 --> 00:07:34,330 +sample okay لسه لسه احكي شغل بطبع نسبة الأشياء + +102 +00:07:34,330 --> 00:07:38,830 +عامة في الشركة اللي بتتحدث عنها مابتدبسوش واقعي و + +103 +00:07:38,830 --> 00:07:42,270 +حتى انا ماريا اتفتت مع شخص على هذا و عادي يعني + +104 +00:07:42,270 --> 00:07:48,140 +سوروه و تمي أشياءال precautions يعني موجود، يعني + +105 +00:07:48,140 --> 00:07:53,240 +في .. في ال .. الجدار بيختفي وراه اللي هو المصور + +106 +00:07:53,240 --> 00:07:57,660 +الأشعة، هذا الجدار باقي، لكن .. يعني خدنا نقول + +107 +00:07:57,660 --> 00:08:01,260 +مابيخدوش الاحتجازات الدورية مائة بالمائة، لكن يصرف + +108 +00:08:01,260 --> 00:08:04,680 +لهم واجبات خصوصي موظفي الأشعة في المستشفيات انا في + +109 +00:08:04,680 --> 00:08:06,840 +فلسطين، يصرف .. يصرف لهم + +110 +00:08:11,310 --> 00:08:14,990 +لكن ربما يكون اهمال من البعض انه ما بيرتزم اما + +111 +00:08:14,990 --> 00:08:19,690 +موجود الكلاملما اتكلمنا عن ال mutation اتكلمنا عن + +112 +00:08:19,690 --> 00:08:23,350 +حزمة spontaneous mutation و عزوناها إلى mistakes + +113 +00:08:23,350 --> 00:08:27,650 +في ال enzymes خلال عملية اللي هو ال replication او + +114 +00:08:27,650 --> 00:08:31,370 +ال transcription و كنا ان ال mutation rate كانت + +115 +00:08:31,370 --> 00:08:35,850 +واحد في عشرة لقوس تمانية كل ميت مليون في انكاني + +116 +00:08:35,850 --> 00:08:40,430 +لطفرة واحدة لكن اتفقنا ان هذا رقم ضخم، رقم ضخم + +117 +00:08:40,430 --> 00:08:44,670 +بالمقارنة مع مين؟ مع الانكسامات العديدة للي بكتيري + +118 +00:08:45,690 --> 00:08:48,810 +mutation and selectivity مفهوم برضه لازم نوضحه + +119 +00:08:48,810 --> 00:08:53,770 +الآن هذا mutation and selectivity تخيلوا أمامنا + +120 +00:08:53,770 --> 00:08:56,790 +على الطاولة هذه على سطح هذا اللي قدامنا في عنا + +121 +00:08:56,790 --> 00:09:01,250 +مليار بكتيريا كلهم حاسسين ل antibiotic اسمه + +122 +00:09:01,250 --> 00:09:06,510 +penicillin تمام؟ ما عدا بكتيريا واحدة استطاعت أن + +123 +00:09:06,510 --> 00:09:10,910 +تكون resistance بطريقة المعهد كل ما انا لهافلو + +124 +00:09:10,910 --> 00:09:14,310 +وضعنا الان احنا البنسيين على الطاولة كل المليار + +125 +00:09:14,310 --> 00:09:17,690 +هيموت مع عدى هذه البكتيريا اخترناها ولا مااخترناها + +126 +00:09:17,690 --> 00:09:22,990 +احنا الان؟ كيف اخترناها؟ بإنه اضفنا المادة القاتلة + +127 +00:09:22,990 --> 00:09:26,910 +للاخرى يعني لا تلاحظوا شغل تاني على الاختيار هذه + +128 +00:09:26,910 --> 00:09:31,350 +البكتيريا ده كانت واحد من المليار تخيلوا انتوا في + +129 +00:09:31,350 --> 00:09:36,110 +الفصل اللي قدامنا الآن خمسين طالبة فرصتكم كنتوا + +130 +00:09:36,110 --> 00:09:40,950 +تسألوني سؤال كل واحدة واحد اتنين في المية يعني2% + +131 +00:09:40,950 --> 00:09:46,890 +الان لو لم تبقى لطالب واحدة الفصل ففرصة نتساءل + +132 +00:09:46,890 --> 00:09:52,310 +السؤال 100% فالان أخدت فرص الآخرين فالبكتيريا + +133 +00:09:52,310 --> 00:09:56,370 +الواحدة اللي موجودة برضه أخدت فرص الآخرين من أي + +134 +00:09:56,370 --> 00:10:00,910 +ناحية من ناحية البيئة الموجودة الغذاء فتكاترت بدون + +135 +00:10:00,910 --> 00:10:05,330 +ما يكون في إلها أي منافسين يبقى اكترناها ولا ما + +136 +00:10:05,330 --> 00:10:10,440 +اكترناهاش selectivityطيب لو قلنالكوا أن مقاومتها + +137 +00:10:10,440 --> 00:10:16,520 +للبنسالين على سبيل المثال نشأت عن طفرة فمين سبب + +138 +00:10:16,520 --> 00:10:21,740 +الاختيار؟ طفرة يعني بمعنى الحكي أن الطفرات قد + +139 +00:10:21,740 --> 00:10:26,960 +تنتخب، قد تختار، قد تميز أنواع معينة من البكتيريا + +140 +00:10:26,960 --> 00:10:30,460 +وتنجحها على الأنواع الأخرى أو أعطيكم مثال أنا بسيط + +141 +00:10:30,460 --> 00:10:36,920 +جدا، لو جيبنا بكتيريا و حطيناها في صحراءسحرة مش + +142 +00:10:36,920 --> 00:10:42,620 +اكل اهو ولا شرب، هتموت من الجوع، نفترض واحدة من + +143 +00:10:42,620 --> 00:10:44,540 +البكتيري الموجودة اللي في اللي حاطناه من هناك + +144 +00:10:44,540 --> 00:10:50,460 +الأرواح استطاعت انها .. هذا مثال readily، بس مجرد + +145 +00:10:50,460 --> 00:10:54,220 +التوضيع، انها تتغذى على الرمل، انه تستخدم الرمل + +146 +00:10:54,220 --> 00:10:57,900 +كمصدر كربوني، كمصدر كربوني، بيطفر + +147 +00:11:06,320 --> 00:11:10,800 +لأ و هذا يحدث حقيقة في البيئات بشكل مستمر لذلك انا + +148 +00:11:10,800 --> 00:11:14,000 +لأ انا دائما بقول ان ما نسميه احنا spontaneous + +149 +00:11:14,000 --> 00:11:17,700 +mutation قد لا يكون spontaneous و قد يكون مبرمج ان + +150 +00:11:17,700 --> 00:11:21,560 +البكتيريا يصير فيها تفرات حتى تتأقل مع الظروف + +151 +00:11:21,560 --> 00:11:25,800 +المتغيرة في هذا العالم طيب ليه مصطلح تاني اسمه + +152 +00:11:25,800 --> 00:11:30,980 +backward mutation تخيلوا البكتيريا هذه اللي صار + +153 +00:11:30,980 --> 00:11:36,720 +فيها mutationوصارت بصفة معينة أو بدون صفة، فقدت + +154 +00:11:36,720 --> 00:11:40,820 +صفة، طبعا؟ صار فيها تاني يوم mutation في نفس ال + +155 +00:11:40,820 --> 00:11:45,360 +gene رجع ال mutation الأولانية إلى ال original، + +156 +00:11:45,360 --> 00:11:48,080 +إيش بدنا نسميها الآن؟ back mutation + +157 +00:11:55,550 --> 00:11:59,410 +وبالتالي رجعت البكتيريا لل wild type او لل + +158 +00:11:59,410 --> 00:12:02,170 +original genotype + +159 +00:12:07,720 --> 00:12:10,520 +ال transformation ما .. ما أظن أنه لأ و محتاج + +160 +00:12:10,520 --> 00:12:13,900 +أتكلم عنها كثيرا لكن في اللوحة اللي أمامكوا في + +161 +00:12:13,900 --> 00:12:18,280 +الفقرة الثانية في أمثلة على أسماء بكتيريا معروفة + +162 +00:12:18,280 --> 00:12:23,180 +أنه قادرة عليها تاخد DNA من ال surrounding واش + +163 +00:12:23,180 --> 00:12:27,220 +تملؤ .. تملؤ incorporation أو recombination يعني + +164 +00:12:27,220 --> 00:12:30,900 +اللي هو دمجوا في المادة الوراثية تبعها زي البصيلاس + +165 +00:12:30,900 --> 00:12:35,440 +و الhemophilus و النسيريا و ال pneumococcus هو اسم + +166 +00:12:35,440 --> 00:12:37,020 +آخر لrestrictococcus pneumonia + +167 +00:12:40,700 --> 00:12:44,720 +هذه الصورة ترينا المادة الورقية اللي هي ال free + +168 +00:12:44,720 --> 00:12:45,460 +DNA + +169 +00:12:48,910 --> 00:12:52,770 +و بعدين بتعملها incorporation داخل ال DNA فال + +170 +00:12:52,770 --> 00:12:57,710 +answer فياندي DNA من جسم غريب أو من خلية غريبة أو + +171 +00:12:57,710 --> 00:13:00,750 +من خلية متبرعة إلى داخل الخلية اللي يسميناها + +172 +00:13:00,750 --> 00:13:05,610 +recipient cell طبعا إذا بتقارنه هاي ال original و + +173 +00:13:05,610 --> 00:13:08,510 +ال original ده بيسميه البكتيريا ال wild + +174 +00:13:12,260 --> 00:13:18,180 +والتانية اللى سموها transformant اللى تم نقل مادة + +175 +00:13:18,180 --> 00:13:23,900 +ورقية جديدة مهمة المصطلحات هذه ان اتعرف علي ال + +176 +00:13:23,900 --> 00:13:27,770 +wild type و ال transformantال conjugation حكينا عن + +177 +00:13:27,770 --> 00:13:31,490 +نوع واحد من ال .. او نوعين من ال conjugation ده + +178 +00:13:31,490 --> 00:13:34,790 +بتذكروا حكينا عن اللي هو ال F positive و ال F + +179 +00:13:34,790 --> 00:13:38,350 +negative و حكينا عن ال HFR ال HFR هو ال high + +180 +00:13:38,350 --> 00:13:41,690 +frequency ال combination اللي هو متعلق بالإيبزوم + +181 +00:13:41,690 --> 00:13:45,450 +فكان لما كانت البلازميد مرتبطة بالجرامزوم + +182 +00:13:52,530 --> 00:13:55,770 +ال Transduction طبعاً العنوان الأساسي كان تبعه ال + +183 +00:13:55,770 --> 00:14:00,310 +bacteriophage و ال bacteriophage اللي أمامكوا يعني + +184 +00:14:00,310 --> 00:14:05,450 +التركيب تبعته واضحة أنه فيه له head رأس و فيه له + +185 +00:14:05,450 --> 00:14:08,570 +اللي هو ال collar أو العنق هذا و فيه له ال tail + +186 +00:14:08,570 --> 00:14:13,750 +fiber طبعا هنشوف تركيب أكتر التسمية bacteriophage + +187 +00:14:13,750 --> 00:14:18,050 +تعطيكوا إحق بإيش؟ بإنه bacteriophage + +188 +00:14:19,850 --> 00:14:23,350 +لقمة لقمة وهي تسمية خاطئة الفيروس أصغر من + +189 +00:14:23,350 --> 00:14:28,150 +البكتيريا لكن كونه تسمية شاعة فبالتالي لا يبدأ على + +190 +00:14:28,150 --> 00:14:30,610 +تغيير الاسم خلاص ده اسمه bacterophage بندله + +191 +00:14:30,610 --> 00:14:35,570 +اختصارا فاج لكن كنتوا يعرفوا ان ال bacterophage + +192 +00:14:35,570 --> 00:14:39,110 +بيقولنا في حاجة اسمها specificity وسبب ال + +193 +00:14:39,110 --> 00:14:42,970 +specificity نجم عن مين؟ على ال tail fibers اللي + +194 +00:14:42,970 --> 00:14:45,610 +بيقلهم ال receptor على ال cell wall تبقى البكتيريا + +195 +00:14:47,390 --> 00:14:52,090 +باختصار what are viruses؟ viruses are viruses that + +196 +00:14:52,090 --> 00:14:56,570 +infect bacteria، لا ننسى إن هما viruses، لكن هذه + +197 +00:14:56,570 --> 00:14:59,990 +الفيروس مقصصة فقط للبكتيريا، يعني they do not + +198 +00:14:59,990 --> 00:15:04,850 +infect human or animals or plants عشان لما أقولكوا + +199 +00:15:04,850 --> 00:15:08,930 +أنه ممكن نستخدم ال bacterium في العلاج، ما تقولوش + +200 +00:15:08,930 --> 00:15:12,390 +طب ما هي بتضرش الإنسان، لأ بتضرش الإنسانعلى طول + +201 +00:15:12,390 --> 00:15:14,910 +الإنسان لو دخلوا .. لو دخلوا الدم بتعرف عليهم على + +202 +00:15:14,910 --> 00:15:17,690 +أنهم أجسام غريبة بيأكلهم بكل سهولة لإن هم ما + +203 +00:15:17,690 --> 00:15:22,750 +بيقدروا يعملوا infection للإنسان طب كيف تلقوا .. + +204 +00:15:22,750 --> 00:15:27,150 +بعملوا .. سؤال .. سؤال و اجاب تذكري أنه مش دايما + +205 +00:15:27,150 --> 00:15:31,530 +احنا بنصل جهاز المناعة، يعني مثلا ممكن على skin + +206 +00:15:33,080 --> 00:15:37,140 +مافيش جهاز منها للاتهابات ممكن نعمل local على ال + +207 +00:15:37,140 --> 00:15:40,940 +tonsils من جوا نعمل spray على ال tonsils ممكن نحط + +208 +00:15:40,940 --> 00:15:45,220 +البكتيريا oral فتشربها ناكلها و تنزل في الأمعاء + +209 +00:15:45,220 --> 00:15:48,260 +تقطر البكتيريا اللي فيها بعيدا عن ال phagocytes و + +210 +00:15:48,260 --> 00:15:53,340 +عن ال macro خلينا + +211 +00:15:53,340 --> 00:16:01,020 +ناخد الشكل هذا للأسف هذا يبدو لو فيه مشكلة + +212 +00:16:01,020 --> 00:16:01,700 +في عرض ال .. + +213 +00:16:05,480 --> 00:16:08,400 +شايفين اللوحة اللى قدامكوا هذه تركيب بسيط جدا + +214 +00:16:08,400 --> 00:16:14,500 +للبكتيروفاج طالبا على الشكل اللى أنا الآن بضغط + +215 +00:16:14,500 --> 00:16:19,580 +عليه هذا الشكل هو اللى بسموه capsid وال capsid + +216 +00:16:19,580 --> 00:16:24,440 +عبارة عن protein envelope مكون من عشرين متلات + +217 +00:16:24,440 --> 00:16:30,180 +بسموه icosahedral اكتبها + +218 +00:16:46,480 --> 00:16:59,460 +بكوزاهيدران بكوزاهيدران بكوزاهيدران + +219 +00:16:59,460 --> 00:17:04,080 +بكوزاهيدران بكوزاهيدران بكوزاهيدران بكوزاهيدران + +220 +00:17:04,080 --> 00:17:06,320 +بكوزاهيدران بكوزاهيدران بكوزاهيدران بكوزاهيدران + +221 +00:17:06,320 --> 00:17:06,500 +بكوزاهيدران بكوزاهيدران بكوزاهيدران بكوزاهيدران + +222 +00:17:06,500 --> 00:17:06,500 +بكوزاهيدران بكوزاهيدران بكوزاهيدران بكوزاهيدران + +223 +00:17:06,500 --> 00:17:06,500 +بكوزاهيدران بكوزاهيدران بكوزاهيدران بكوزاهيدران + +224 +00:17:06,500 --> 00:17:06,500 +بكوزاهيدران بكوزاهيدران بكوزاهيدران بكوزاهيدران + +225 +00:17:06,500 --> 00:17:10,290 +بكوزاهيدرانالصحيفي عندي في ال .. في ال .. الوزر + +226 +00:17:10,290 --> 00:17:13,650 +الصفلي تركيبين مهمين جدا اللي هم ال tail fibers و + +227 +00:17:13,650 --> 00:17:16,170 +ال tail fibers كمان مرة هم المسئولي عن مين + +228 +00:17:19,160 --> 00:17:23,260 +اللي هو الالتصاق مع ال receptors وفي حسمة base + +229 +00:17:23,260 --> 00:17:29,580 +plate ال base plate عبارة عن hooks زي خطافات تلتصق + +230 +00:17:29,580 --> 00:17:33,260 +بالجدار وهذا الجدار او هذا الجزء بيحتوي على + +231 +00:17:33,260 --> 00:17:38,660 +انزيمات حاضمة انزيمات حاضمة لجدار البكتيريا عشان + +232 +00:17:38,660 --> 00:17:42,060 +يصير يعمل فيه ايه injection لالمادة البرادفة + +233 +00:17:48,590 --> 00:17:53,050 +في حياتنا المحاضرة السابقة، اتعرضنا لدمائي من ال + +234 +00:17:53,050 --> 00:17:57,410 +bacterium فعشان، ال lightic و ال lysogenic، ال + +235 +00:17:57,410 --> 00:18:00,670 +lightic اللي دخل على الخلية و قالهم أنتم الآن تحت + +236 +00:18:00,670 --> 00:18:04,090 +سيطراتك، و حدر الخلية و دمرها و انتهى، هذا اسمه + +237 +00:18:04,090 --> 00:18:08,550 +lightic أو virulent فعشان، virulent يعني شرس + +238 +00:18:14,940 --> 00:18:18,360 +و طبعا واحد كان مسئول عن ال specialized + +239 +00:18:18,360 --> 00:18:20,860 +transduction اللي هو التحتاني و الفجر اللي كان + +240 +00:18:20,860 --> 00:18:23,520 +مسئول عن مين؟ عن ال generalized transduction + +241 +00:18:26,640 --> 00:18:30,300 +بنقدر نلخص ال life cycle ب 4 خطوات رئيسية + +242 +00:18:30,300 --> 00:18:34,120 +absorption او attachment absorption او attachment + +243 +00:18:34,120 --> 00:18:37,840 +بعدين penetration للاختراق بعدين ال biological + +244 +00:18:37,840 --> 00:18:42,360 +synthesis و كلمة biological synthesis جملة بسيطة + +245 +00:18:42,360 --> 00:18:46,600 +لكن فيها تفاصيل ضخمة جدا محاولوشها لما اتكلم عن + +246 +00:18:46,600 --> 00:18:50,620 +الفيروسات و هتكلم عن ال viral replication هلاجي + +247 +00:18:50,620 --> 00:18:55,140 +حالة ان اكبر جزءاللي احنا مختصرينه بجملة واحدة + +248 +00:18:55,140 --> 00:18:58,040 +اللي هو biological synthesis فده ينطق الجملة + +249 +00:18:58,040 --> 00:19:05,300 +الأخيرة maturation أو assembly ايش مادة .. ايش + +250 +00:19:05,300 --> 00:19:09,800 +مادة maturation نضوج .. نضوج او assembly و اللي هو + +251 +00:19:09,800 --> 00:19:15,760 +ال release الخطوة الأولى عسارية كارتون هي عبارة عن + +252 +00:19:15,760 --> 00:19:21,160 +فقط ارتباط بال receptors وسمينها absorption او + +253 +00:19:21,160 --> 00:19:25,530 +attachmentالخطوة التانية ال penetration لاحظوا ان + +254 +00:19:25,530 --> 00:19:29,670 +ال viral genome دخل الى داخل الخلية لكن الفيروس + +255 +00:19:29,670 --> 00:19:34,130 +نفسه الهيكل الخارجي بيبقى بالخارج هذا طبعا اعتبره + +256 +00:19:34,130 --> 00:19:38,070 +صدافة خالية من الحياة خاصة يعني مالوش اي قيمة + +257 +00:19:38,070 --> 00:19:42,390 +مالوش اي قيمة الخطوة التالتة ال biological + +258 +00:19:42,390 --> 00:19:45,910 +synthesis بيتصنية اللي هي الفيروسات كاملة بعد ذلك + +259 +00:19:45,910 --> 00:19:46,210 +اللي هو + +260 +00:19:51,120 --> 00:19:55,480 +اللي قدامكوا الآن هي عبارة عن تلخيص لكل العملية ال + +261 +00:19:55,480 --> 00:19:58,320 +bacteriophage بيعمل attachment أو بناء absorption + +262 +00:19:58,320 --> 00:20:02,660 +بعدين penetration + +263 +00:20:02,660 --> 00:20:05,320 +للمادة الوراطية بعدين اللي هو biological + +264 +00:20:05,320 --> 00:20:10,720 +synthesis, maturation and release وهذه العملية أدت + +265 +00:20:10,720 --> 00:20:17,120 +إلى تحلل الخلايا الخلايا البактерية وإطلاق الفيروس + +266 +00:20:18,220 --> 00:20:22,180 +الان امرأت باننا نستغل هذا المعلومات او هذه + +267 +00:20:22,180 --> 00:20:27,060 +المعلومات في تطبيق مهم جدا في ال microbiology وهو + +268 +00:20:27,060 --> 00:20:34,260 +عد الفيروسات من هذا النوع احنا زي ما بنشتري منتجات + +269 +00:20:34,260 --> 00:20:38,760 +كثيرة بنشتري فيروسات و بنشتري بكتيري و بنبيع و + +270 +00:20:38,760 --> 00:20:44,230 +بنشتريها و لما بنبيع بنقول انا بنبيعك مليونبيروس + +271 +00:20:44,230 --> 00:20:49,330 +او ببيعك أمبوبا فيها مليار بيروس فانا بتأكد من هذا + +272 +00:20:49,330 --> 00:20:54,470 +او بدي اصنع رقم معين ففي الآخر بدي اعد يعني بلزمني + +273 +00:20:54,470 --> 00:21:00,590 +اعمل count بلزمنا نعد ال bacteriophages كيك ممكن + +274 +00:21:00,590 --> 00:21:03,250 +اعد ال bacteriophages اللي من هذا النوع اللي هي ال + +275 +00:21:03,250 --> 00:21:08,690 +lytic phages تركزوا معاه عشان نوضح الصورة بشكل جيد + +276 +00:21:10,160 --> 00:21:13,160 +تخيلوا معايا طبق .. طبق اللي في المعمر، شفتمه؟ و + +277 +00:21:13,160 --> 00:21:14,200 +لا .. و لا .. و لا .. و لا .. و لا .. و لا .. و لا + +278 +00:21:14,200 --> 00:21:14,260 +.. و لا .. و لا .. و لا .. و لا .. و لا .. و لا .. + +279 +00:21:14,260 --> 00:21:14,420 +و لا .. و لا .. و لا .. و لا .. و لا .. و لا .. و + +280 +00:21:14,420 --> 00:21:14,800 +لا .. و لا .. و لا .. و لا .. و لا .. و لا .. و لا + +281 +00:21:14,800 --> 00:21:14,820 +.. و لا .. و لا .. و لا .. و لا .. و لا .. و لا .. + +282 +00:21:14,820 --> 00:21:15,600 +و لا .. و لا .. و لا .. و لا .. و لا .. و لا .. و + +283 +00:21:15,600 --> 00:21:17,080 +لا .. و لا .. و لا .. و لا .. و لا .. و لا .. و لا + +284 +00:21:17,080 --> 00:21:17,760 +.. و لا .. و لا .. و لا .. و لا .. و لا .. و لا .. + +285 +00:21:17,760 --> 00:21:17,780 +و لا .. و لا .. و لا .. و لا .. و لا .. و لا .. و + +286 +00:21:17,780 --> 00:21:22,600 +لا .. و لا .. و لا .. و لا .. و لا .. و لا .. و لا + +287 +00:21:22,600 --> 00:21:28,180 +.. و لا .. و لا .. + +288 +00:21:38,920 --> 00:21:41,520 +الأمبوبا اللى فيها ال bacteriophage و بنعدها + +289 +00:21:41,520 --> 00:21:44,940 +بنعملها dilution تخيل ولا بنتكلم عن مليار ولا + +290 +00:21:44,940 --> 00:21:50,840 +مليون لو قلتلك قد من واحد لمليار هتموتى و انت + +291 +00:21:50,840 --> 00:21:53,960 +تقولى تسعمية و تسعة و خمسين ألف يمكن او .. يعني مش + +292 +00:21:53,960 --> 00:21:59,790 +هنخلص ولا لأ؟فإيش هنعمل؟ هنعمل dilution وعمل ال + +293 +00:21:59,790 --> 00:22:03,250 +dilution بيعتمد على ال original number على ال + +294 +00:22:03,250 --> 00:22:05,570 +original number جدش ال original number اللي موجود + +295 +00:22:05,570 --> 00:22:11,010 +مش عارفينه هنا إذا متوقع ممكن نعمل مثلا dilution + +296 +00:22:11,010 --> 00:22:14,430 +كتير إذا عارفين بالضبط ال original بنعمل dilution + +297 +00:22:14,430 --> 00:22:19,490 +على جده بحاجة إنه في الآخر نحصل على 100 فيروس إلى + +298 +00:22:19,490 --> 00:22:25,410 +200 فيروس تمام؟ نقدر نقدهم، مظبوط؟عملنا dilution + +299 +00:22:25,410 --> 00:22:29,690 +في القنبوبة و من القنبوبة الأخيرة أخدنا واحد مل و + +300 +00:22:29,690 --> 00:22:34,850 +فرضناه على البكتيريا، الآن الواحد مل نفترض .. + +301 +00:22:34,850 --> 00:22:40,890 +نفترض طلع على الطبق تاني يوم خمسين + +302 +00:22:40,890 --> 00:22:44,710 +.. خمسين فراغ، إيش هنا خمسين فراغ؟ + +303 +00:22:55,280 --> 00:22:58,160 +هيقرصهم بس شوية لو طلبنا انه البكتيريا كيف نام + +304 +00:22:58,160 --> 00:23:06,680 +البكتيريا مش شايفينها؟ في + +305 +00:23:06,680 --> 00:23:12,180 +لومباد صح؟ على الصبورة، هلأ تقولوا نمو البكتيريا، + +306 +00:23:12,180 --> 00:23:15,080 +لو كان فيه بكتيروفاج المنطقة هالي، ايش هيعمل + +307 +00:23:15,080 --> 00:23:18,600 +البكتيريا قلناها؟ هيحللها، هيحللها و هينتقل لخلايا + +308 +00:23:18,600 --> 00:23:24,280 +مجاورة، هيحللها، فبلاقل هلاجي منطقة يشملهاهذه + +309 +00:23:24,280 --> 00:23:28,140 +المنطقة الفراعات بنسميها black، black منطقة خالية + +310 +00:23:28,140 --> 00:23:33,200 +من النمو، نتيجة تحلل البكتيريا من قبل الفيروس، + +311 +00:23:33,200 --> 00:23:37,180 +مثلا هو موضح في هذه الصورة ده، تشتركينا أمامكوا، + +312 +00:23:37,180 --> 00:23:41,160 +موضح في الصورة هذه إنه في نمو وبعدين في الناقل هو + +313 +00:23:41,160 --> 00:23:46,580 +الفراعات، إذا لو عدينا خمسين فراعات، خمسين فراعات + +314 +00:23:46,580 --> 00:23:52,230 +من أي dilutionمن الأخير مولا افترض انه كان عشرة + +315 +00:23:52,230 --> 00:23:57,170 +أقص ناقص ستة عشرة أقص ناقص ستة خمسين ضرب عشرة أقص + +316 +00:23:57,170 --> 00:24:01,890 +ستة بطلع الرقم الحقيقي تبعنا ايه الوحدات اللي + +317 +00:24:01,890 --> 00:24:08,170 +بنحطها؟ بنحط وحدة هذا في البكتيريا لما نقعد في + +318 +00:24:08,170 --> 00:24:11,290 +البكتيريا cooling forming unit لكن في الفيروسات + +319 +00:24:11,290 --> 00:24:16,930 +بسميها PFU plaque forming unit PFU + +320 +00:24:20,340 --> 00:24:29,500 +ليش ماقولناش الجوهدات bacteriophage ليش بقولنا BFU + +321 +00:24:29,500 --> 00:24:33,920 +Black Forming Area خدوا بالكم النقطة احنا عدينا + +322 +00:24:33,920 --> 00:24:39,000 +bacteriophage ولا عدينا blacks طيب احنا ال blacks + +323 +00:24:39,000 --> 00:24:45,180 +بنفترض ان كل black ناشق عن عن بيروس واحد عن بيروس + +324 +00:24:45,180 --> 00:24:49,610 +واحد هل هذا الافتراض حقيقي؟طب لو كانوا اتنين اللي + +325 +00:24:49,610 --> 00:24:54,670 +و لزجوا مع بعض و عملونا black واحد فعشان انا ادعى + +326 +00:24:54,670 --> 00:24:58,050 +الدقة في ال micro biology فبروح و انا حاطط بالفعل + +327 +00:24:58,050 --> 00:25:03,310 +وصف لما قمت بعمله بدل ما اقول خمسين ضرب عشرة لقوا + +328 +00:25:03,310 --> 00:25:09,010 +ستة viruses او بكتيروفاج بقول خمسين ضرب عشرة لقوا + +329 +00:25:09,010 --> 00:25:13,490 +ستة black forming unit ال unit ممكن تكون virus + +330 +00:25:13,490 --> 00:25:15,850 +واحد ممكن ماتكوناش فعلا virus واحد لكن في العالم + +331 +00:25:15,850 --> 00:25:21,840 +الغالبفي ال microvirus بينظروا انها كل black انه + +332 +00:25:21,840 --> 00:25:26,740 +virus طب هذه النقطة مهمة جدا من النقطة هذه ان احنا + +333 +00:25:26,740 --> 00:25:31,000 +نعدل الفيروسات مهم جدا ان نعدل الفيروسات زي ما مهم + +334 +00:25:31,000 --> 00:25:36,200 +جدا ان نعدل ال bacteriaهذا سؤال لو سأله حطيته + +335 +00:25:36,200 --> 00:25:40,660 +وسؤال النقاش لماذا ما هي تطبيقات لعد ال فيروسات + +336 +00:25:40,660 --> 00:25:44,220 +ارجو منكم تتفاعلوا علشان الآن طبعا على الموقع + +337 +00:25:44,220 --> 00:25:47,540 +الفيسبوك ايش النقاط اللي ممكن نستفيد منها في عد + +338 +00:25:47,540 --> 00:25:56,460 +اللي هو الفيروسات النوع + +339 +00:25:56,460 --> 00:25:59,940 +التاني من الفيروسات المعنونه اللي هو ال ال ال ال + +340 +00:25:59,940 --> 00:26:04,230 +lysogenic أو ال temperateبرضه attachment او + +341 +00:26:04,230 --> 00:26:08,690 +absorption penetration + +342 +00:26:08,690 --> 00:26:17,330 +الان لأ الان هيعملش الان integration صح بروفاج + +343 +00:26:17,330 --> 00:26:19,970 +formation اللى يسمى انها integration و ال virus + +344 +00:26:19,970 --> 00:26:24,810 +بيقول لل DNA انا بدي ا .. بس ماكان المفروض ان + +345 +00:26:24,810 --> 00:26:28,070 +البكتيريا في هذه اللحظة إذا بتنقسم .. إذا بتنقسم + +346 +00:26:28,070 --> 00:26:34,000 +هينقسم معاها تمام؟ملخص العملية انه يكون بروفاج بس + +347 +00:26:34,000 --> 00:26:41,080 +لحد الان تكون بروفاج نكمل الان الفيروس قرر لسبب ما + +348 +00:26:41,080 --> 00:26:46,100 +انه ينفصل ينسحب انا قولتكوا السبب .. السبب ما هذا + +349 +00:26:46,100 --> 00:26:50,460 +في الغالب ايه induction ايش هال induction يعني في + +350 +00:26:50,460 --> 00:26:55,400 +عوامل معروفة في ال micro biology مثل الفيروس منها + +351 +00:26:55,400 --> 00:27:01,640 +شؤون الفيروسالشهور كلمة شهور مش يعني اللي هتطبق + +352 +00:27:01,640 --> 00:27:06,260 +على الفيروسات لكن خلينا نقول قالية وميكانيزم by + +353 +00:27:06,260 --> 00:27:09,580 +which the virus senses that the bacteria is dying + +354 +00:27:09,580 --> 00:27:15,500 +البكتيريا تقرض للموضوع، بينفصل طبعا في الآخر برجع + +355 +00:27:15,500 --> 00:27:19,360 +و بيكون نفس الفكرة اللي عملها ال lightick phage + +356 +00:27:19,360 --> 00:27:22,140 +بيعمل الآن biological synthesis اللي هي قدام + +357 +00:27:22,140 --> 00:27:25,400 +العملية و بعد ذلك اللي هي ال release + +358 +00:27:28,890 --> 00:27:32,490 +لنفس البكتيريا لأ مش هو سحب ال .. ال .. ال .. ال + +359 +00:27:32,490 --> 00:27:32,750 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +360 +00:27:32,750 --> 00:27:33,230 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +361 +00:27:33,230 --> 00:27:34,670 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +362 +00:27:34,670 --> 00:27:34,670 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +363 +00:27:34,670 --> 00:27:37,770 +.. ال .. ال .. + +364 +00:27:37,770 --> 00:27:41,430 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +365 +00:27:41,430 --> 00:27:41,490 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +366 +00:27:41,490 --> 00:27:41,650 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +367 +00:27:41,650 --> 00:27:42,950 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +368 +00:27:42,950 --> 00:27:43,170 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +369 +00:27:43,170 --> 00:27:43,170 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +370 +00:27:43,170 --> 00:27:44,210 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +371 +00:27:44,210 --> 00:27:54,370 +.. ال .. ال .. ال .. ال .. ال + +372 +00:28:05,460 --> 00:28:07,900 +الان خلق .. هي كده .. هي كده الـ profile و لا يزور + +373 +00:28:07,900 --> 00:28:12,020 +نوع ال cell قرر انه ينفصل في نفس البكتيريا عمل + +374 +00:28:12,020 --> 00:28:18,320 +biological synthesis و + +375 +00:28:18,320 --> 00:28:21,120 +بعدين صارله maturation و بعدين بيصيرله اللي هو + +376 +00:28:21,120 --> 00:28:25,200 +release يبقى العملية بالتم كلها في بكتيريا طبعا + +377 +00:28:25,200 --> 00:28:27,040 +البكتيريا الواحدة أقصد فيها اللي هي ال .. ال + +378 +00:28:27,040 --> 00:28:29,640 +progeny اللي هي السلالة اللي نشأت من ال .. من + +379 +00:28:29,640 --> 00:28:31,440 +الخلية الأولى ال original cell + +380 +00:28:36,610 --> 00:28:40,410 +What are the applications of bacteriophages؟ واحد، + +381 +00:28:40,410 --> 00:28:42,430 +وحكيناهم كلها تاخدوا بالكم، بس بدنا نعيدهم الآن + +382 +00:28:42,430 --> 00:28:45,990 +وندكركمهم بشكل نقاط، واحد، treatment of bacterial + +383 +00:28:45,990 --> 00:28:54,230 +infection، وانا بقولكوا يعني نموذج يمكن قديم جدا، + +384 +00:28:54,230 --> 00:28:58,990 +الموضوع مش جديد، الروس أو في روسيا اهتموا بهذا + +385 +00:28:58,990 --> 00:29:02,350 +الموضوع منذ أو أول القرن العشرين، يعني في الف + +386 +00:29:02,350 --> 00:29:06,780 +وتسعمائة وإشي، بدوا يهتموا بهذا الموضوعاكتشاف ال + +387 +00:29:06,780 --> 00:29:13,020 +antibiotics في أربعينيات القرن الماضي خلّى العالم + +388 +00:29:13,020 --> 00:29:16,940 +الغربي ينسى موضوع ال viruses ويركز على موضوع ال + +389 +00:29:16,940 --> 00:29:22,360 +antibiotics لأن ال antibiotics بالفعل كانت طفرة + +390 +00:29:22,360 --> 00:29:25,700 +يعني لحد .. ل .. ل .. ل .. لدرجة انه المؤرخين + +391 +00:29:25,700 --> 00:29:30,580 +الطبيين بسموا عصر الماضي .. القرن الماضي عصر + +392 +00:29:30,580 --> 00:29:36,140 +المغضب الحيويليش؟ لأنه أحدثت قفزة في ال .. في + +393 +00:29:36,140 --> 00:29:40,660 +العلاجات وفي الطب احنا دلوقتي كانوا بيموتوا من .. + +394 +00:29:40,660 --> 00:29:43,800 +من simple .. من simple tonsillitis او urinary + +395 +00:29:43,800 --> 00:29:46,900 +tract infection اليوم لأ ال antibiotics بالفعل + +396 +00:29:46,900 --> 00:29:50,420 +قذلت منسبة الوفيات في أمراض كتيرة حسنت من + +397 +00:29:50,420 --> 00:29:53,900 +العمليات، نجاح العمليات الجراحي لأنه فجأة زمان + +398 +00:29:53,900 --> 00:29:57,680 +تنجح العملية ويموت المريض من التهابات نا بعد + +399 +00:29:57,680 --> 00:30:01,880 +العملية فال antibiotic أحدثت قفزة كبيرةفالعالم + +400 +00:30:01,880 --> 00:30:05,400 +الغربي تقريبا نسي موضوع ال bacteriophages وركز كله + +401 +00:30:05,400 --> 00:30:09,500 +على مين، لكن الروس ضالهم شغالين، لكن كونوا الروس + +402 +00:30:09,500 --> 00:30:13,700 +.. عمركوا قرأتوا مقالة بالروس ينتوا مثلا؟ لأ ليش؟ + +403 +00:30:13,700 --> 00:30:16,220 +لأن اللغة تبعتهم لم تنتشر كما انتشرت اللغة + +404 +00:30:16,220 --> 00:30:20,200 +الأيبيزية في العالم، الآن و بعد ظهور سلالات مقاومة + +405 +00:30:20,200 --> 00:30:26,480 +للمضادات الحيوية و بوادر أنه احنا خسرين المعركة مع + +406 +00:30:26,480 --> 00:30:30,160 +المضادات الحيوية، بدأ العالم الغربي مرة أخرى يعيد + +407 +00:30:30,160 --> 00:30:35,110 +الاهتمامبموضوع اللي هو ال bacteriophage وفيه زميل + +408 +00:30:35,110 --> 00:30:39,590 +او دكتورة من السعودية اعتقد انها فلسطينية من غزة + +409 +00:30:39,590 --> 00:30:44,630 +من هنا عملت دراسة على bacteriophage ل .. عزفت + +410 +00:30:44,630 --> 00:30:47,790 +bacteriophage لقتل وستنموه في العلاج في السعودية + +411 +00:30:47,790 --> 00:30:52,130 +لقتل ال metasilin resistance typhilococcus aureus + +412 +00:30:52,130 --> 00:30:55,610 +ونجحوا في ذلك وكانت هذه رسالة دكتوراه بهذه + +413 +00:30:55,610 --> 00:30:58,590 +الدكتوراه ففي عندهم potential + +414 +00:31:02,360 --> 00:31:07,720 +مستقبلية لاستخدام وتوظيف البكتيروفاجز في علاج بعض + +415 +00:31:07,720 --> 00:31:14,340 +الأمراض وهما يميزها كمان مرة ل specificity يعني + +416 +00:31:14,340 --> 00:31:18,480 +بنقتل السلمونيلا دون أن نضرب ال normal flora انت + +417 +00:31:18,480 --> 00:31:22,860 +بيتك احد عيوو وقاش انه بيقتل ال pathogen و ال + +418 +00:31:22,860 --> 00:31:26,340 +monkey pathogen وهذا بيملي خلل في مين؟ في ال + +419 +00:31:26,340 --> 00:31:30,330 +normal floraهدل على إنه هذا .. إنه .. إنه ال + +420 +00:31:30,330 --> 00:31:33,270 +bacterial phage يعني ماعندهوش أي عيوب، لأ أحد + +421 +00:31:33,270 --> 00:31:36,210 +عيوب، و اللي حاكيه اللي أنا هجيبه شوية، إنه لو دخل + +422 +00:31:36,210 --> 00:31:39,670 +رسم الإنسان في الدم يا مام، رسم الإنسان بتعامل + +423 +00:31:39,670 --> 00:31:43,310 +فعاليا كرسم غريب و بي .. بيكسره، وبالتالي مش هيكون + +424 +00:31:43,310 --> 00:31:48,690 +فعال في علاج كل أنواع ال infection في رسم الإنسان، + +425 +00:31:48,690 --> 00:31:53,160 +نمر اتنين في الفوائد أو التطبيقات usedin the + +426 +00:31:53,160 --> 00:31:57,040 +identification of pathogenic bacteria ومصطلح سمنها + +427 +00:31:57,040 --> 00:32:03,380 +phage أو phage type كونه بينقل جينات عفوا من خلية + +428 +00:32:03,380 --> 00:32:07,440 +إلى خلية فقد يستخدم في ال molecular biology + +429 +00:32:07,440 --> 00:32:08,140 +research + +430 +00:32:20,350 --> 00:32:23,130 +لحظة يا أفندي، اللي هو حادة، بس لحظة اللي هي الـ + +431 +00:32:23,130 --> 00:32:25,790 +lysogenized أو الـ lysogenic conversion احنا + +432 +00:32:25,790 --> 00:32:30,510 +اتفقنا على إنه وجود profage في البكتيريا بيعطي + +433 +00:32:30,510 --> 00:32:35,830 +البكتيريا اسم lysogenized bacteria الآن هل لل + +434 +00:32:35,830 --> 00:32:40,350 +lysogenized bacteria أي أهمية في ال medical + +435 +00:32:40,350 --> 00:32:45,170 +microbiology؟ نعم، هناك أهمية كبرى، لهذا المصطلح + +436 +00:32:45,170 --> 00:32:48,490 +في ال medical microbiology وأنا ذكرت مثلًا المرة + +437 +00:32:48,490 --> 00:32:52,720 +الماضيةفاكرينه ولا مش فاكرينه؟ اللي هو ال + +438 +00:32:52,720 --> 00:32:57,920 +corebacterium diphtheria هذه بكتيريا غير سامة لما + +439 +00:32:57,920 --> 00:33:03,680 +بيصير lysogenized بالفعش تبعها بتنتج توكسينو سموها + +440 +00:33:03,680 --> 00:33:07,300 +toxigenic corebacterium diphtheria ولو عزلت + +441 +00:33:07,300 --> 00:33:10,500 +corebacterium diphtheria من المريض لأ وعرفتها بيو + +442 +00:33:10,500 --> 00:33:13,640 +كيميائية عنها corebacterium diphtheria لازم اتأكد + +443 +00:33:13,640 --> 00:33:18,990 +منها هل هي toxigenic ولا non-toxigenicهي أهمية لل + +444 +00:33:18,990 --> 00:33:24,130 +Lysogenic Conversion و هناك ميكروبات أخرى زي ال + +445 +00:33:24,130 --> 00:33:27,030 +Plastridium Botulonium اللى بتنتج أقوى سم عارف + +446 +00:33:27,030 --> 00:33:31,550 +للإنسان ايضا هى ناتجة عن Lysogenic Conversion + +447 +00:33:31,550 --> 00:33:37,910 +ببكتيريو فعالى طب بتعطيكوا كمان واحدة خطيرة جدا هل + +448 +00:33:37,910 --> 00:33:43,010 +سمعتوا ببكتيريا أكلت اللحم؟ ايش اسمته عنها يعني؟ + +449 +00:33:43,010 --> 00:33:43,470 +كل خير + +450 +00:33:46,600 --> 00:33:49,560 +هذه البكتيريا لو أصارت على سبيل المثال كتف واحد + +451 +00:33:49,560 --> 00:33:55,880 +ممكن بعد يوم من infection نشوف العضم هي مش بتاكل + +452 +00:33:55,880 --> 00:33:59,720 +لحم بمعنى بتاكل لحم وإنما عندها المقدر على هضم + +453 +00:33:59,720 --> 00:34:04,780 +لبروتينات بشكل مش طبيعي عندها proteinases كمية + +454 +00:34:04,780 --> 00:34:10,680 +proteinases كبيرة جدا وبالتالي قدرتها على هضم + +455 +00:34:10,680 --> 00:34:13,980 +لبروتينات و تذكروا أن ال muscle سبعتنا أو ال flesh + +456 +00:34:13,980 --> 00:34:18,960 +سبعتنا هو ايضا عن بروتينفعطاها قدرة و البكتيريا + +457 +00:34:18,960 --> 00:34:23,560 +هذه مشهورة جدا جدا جدا و بتصيبنا احنا باستمرار + +458 +00:34:23,560 --> 00:34:28,200 +اسمها streptococcus biogenes تماما لواز مين منكم + +459 +00:34:28,200 --> 00:34:32,760 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+00:35:00,570 --> 00:35:02,070 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +472 +00:35:02,070 --> 00:35:02,150 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +473 +00:35:02,150 --> 00:35:02,150 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +474 +00:35:02,150 --> 00:35:02,150 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +475 +00:35:02,150 --> 00:35:02,150 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +476 +00:35:02,150 --> 00:35:02,150 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +477 +00:35:02,150 --> 00:35:02,150 +ال ال ال ال ال ال ال ال ال + +478 +00:35:11,480 --> 00:35:16,820 +خليتين ببعضهم البعض على أما الحصول على خلية واحدة + +479 +00:35:16,820 --> 00:35:20,860 +تحتوي على خصائص من الخليتين التانية هعطيكوا مثال + +480 +00:35:20,860 --> 00:35:26,020 +على protoplast fusion خارج نطاق البكتيريا بتسمعوا + +481 +00:35:26,020 --> 00:35:32,200 +بال antibody و بتسمعوا بال monoclonal antibody ال + +482 +00:35:32,200 --> 00:35:36,520 +monoclonal + +483 +00:35:36,520 --> 00:35:40,420 +antibody هي عبارة عن antibody موجهة ضد EV2 بواحد + +484 +00:35:40,420 --> 00:35:46,110 +فقط لهاEpitope يعني جزء من أنتجن، مش أنتجن، جزء من + +485 +00:35:46,110 --> 00:35:50,510 +أنتجن بسموه محددات الانتجين، antigen determinants + +486 +00:35:50,510 --> 00:35:55,150 +أو Epitope هذا الكلام في الجسم مستحيل نحصل على + +487 +00:35:55,150 --> 00:36:00,650 +monoclonal الانتجن بدخل جسم يكون له أجسام مضادة، + +488 +00:36:00,650 --> 00:36:03,970 +مش جسم واحد، مش monoclonal، مش سلالة واحدة، + +489 +00:36:03,970 --> 00:36:09,950 +سلالات، لأن الانتجن بيكون أكتر من Epitopeففي + +490 +00:36:09,950 --> 00:36:13,950 +الخارج من الجسم بنعمل تقنية اسمها hybridoma + +491 +00:36:13,950 --> 00:36:18,050 +technique hybridoma كلمة hybrid ايش يعني hybrid؟ + +492 +00:36:18,050 --> 00:36:25,350 +تهجين بنجيب خلية سلطانية و بنجيب B lymphocyte ال B + +493 +00:36:25,350 --> 00:36:28,590 +lymphocyte دي بنكون اختارناها احنا و عارفين انها + +494 +00:36:28,590 --> 00:36:32,330 +تنتج هذا ال antibody اللي بدنا هيها عارفين كده اش + +495 +00:36:32,330 --> 00:36:37,030 +عمرها ال B lymphocyte؟ ساعات، ست ساعات، خارج الجسم + +496 +00:36:37,030 --> 00:36:42,730 +و بعدين بتموتhybridoma او carcinoma cell بتعيش + +497 +00:36:42,730 --> 00:36:45,810 +forever و افريلها اكل و شرب و جي معاها ال waste + +498 +00:36:45,810 --> 00:36:50,610 +تمام؟ بتعيش forever يبقى ايش الميزة بتاعتها؟ ال + +499 +00:36:50,610 --> 00:36:56,570 +survival تبعها forever التانية short-lived ايش + +500 +00:36:56,570 --> 00:36:59,170 +ميزتها ال short-lived؟ انها بتنتج normal antibody + +501 +00:36:59,170 --> 00:37:03,660 +انا بديها التانية بتنتج normal antibodyيبقى في + +502 +00:37:03,660 --> 00:37:07,800 +واحدة فيها ميزة جيدة وفيها قيب والتانية فيها قيب + +503 +00:37:07,800 --> 00:37:11,900 +وفيها ورضه ميزة، روحت نفشتهم مع بعض، bulliated in + +504 +00:37:11,900 --> 00:37:15,740 +a glycol، فاكريني ما حكينا المرة الماضية، ال pig، + +505 +00:37:15,740 --> 00:37:20,710 +فطلعولي خالية اسمها ال Bradova cell بتعيش foreverو + +506 +00:37:20,710 --> 00:37:24,810 +بتنتج ل normal antibodies و الآن معظم ال reagents + +507 +00:37:24,810 --> 00:37:27,730 +اللي بتستخدموهم في المختبرات و فيها antibodies + +508 +00:37:27,730 --> 00:37:32,110 +تمام و ياخدوا بالكوا هذا التقنية اللي هي تقنية ان + +509 +00:37:32,110 --> 00:37:35,610 +انا استخدم antibody للكشف او لقياس أشياء متوفرة + +510 +00:37:35,610 --> 00:37:39,590 +عندنا في التحيات الطبية بكمية كبيرة أنواع كثيرة من + +511 +00:37:39,590 --> 00:37:44,710 +التحوصات متبدة على هذه التقنيةفاللي بنجح إنه يعمل + +512 +00:37:44,710 --> 00:37:48,850 +hybridoma تقنيك .. hybridoma لAntibody، بيصير عنده + +513 +00:37:48,850 --> 00:37:52,590 +مصنع بينتج من خلاله ال antibodies و بيوزعه على + +514 +00:37:52,590 --> 00:37:54,730 +الشركات اللي بتستغل هذا ال antibody في ال + +515 +00:37:54,730 --> 00:37:57,430 +diagnostic أو في ال treatment أو في خلاله، إذا ال + +516 +00:37:57,430 --> 00:38:02,470 +Protoplastin فيه ايضا تقنية جيدة و ممتازةالسؤال .. + +517 +00:38:02,470 --> 00:38:06,390 +السؤال ال .. ال .. المحاضرة اللي هو how could we + +518 +00:38:06,390 --> 00:38:08,670 +benefit from bacterial genetics أنا لا أقصد ال + +519 +00:38:08,670 --> 00:38:11,430 +bacterial genetics ال .. ال .. ال .. نفسها إنه + +520 +00:38:11,430 --> 00:38:14,730 +المحاضرة تبعتنا، محاضرة ال bacterial genetics كيف + +521 +00:38:14,730 --> 00:38:18,330 +أنا أستفيد من هذه المحاضرة المحاضرة القادمة إن شاء + +522 +00:38:18,330 --> 00:38:26,790 +الله هتكون عن موضوع نقدمله + +523 +00:38:26,790 --> 00:38:30,790 +بس تقديم بسيط صغير اسمه sterilization and disease + +524 +00:38:33,950 --> 00:38:36,810 +تحت عنوان بنقدر نسميه احنا control of + +525 +00:38:36,810 --> 00:38:41,950 +microorganism، خدوا بالكم، علمنا كيف نربيها، نربي + +526 +00:38:41,950 --> 00:38:45,910 +البكتيريا نميها، نحط لها الغذاء و ال بي إي أتش + +527 +00:38:45,910 --> 00:38:49,770 +مناسب و حرارة مناسبة و أكسوجين و CO2 و إلا .. الآن + +528 +00:38:49,770 --> 00:38:55,270 +صار عندنا ما عارفة بتنميتهاتنميتها فيش أسهل منها + +529 +00:38:55,270 --> 00:38:59,890 +المشكلة هو يعني تسيطر عليها تخيلوا الغرفة دي اللي + +530 +00:38:59,890 --> 00:39:03,270 +احنا قاعدين فيها بدنا نتخلص من كل الميكروبات هذه + +531 +00:39:03,270 --> 00:39:08,750 +مهمة صعبة جدا جدا جدا فبدنا نتعلم الطرق المستخدمة + +532 +00:39:08,750 --> 00:39:12,310 +في التعقيم و التطهير و دايما كلمة تعقيم مصطلح + +533 +00:39:12,310 --> 00:39:16,490 +بنسميه أحنا absolute ايش أنت absolute؟ مطلق وال + +534 +00:39:16,490 --> 00:39:20,190 +absolute killing of all forms of life فده بدنا + +535 +00:39:20,190 --> 00:39:24,700 +نعقم الإيدالتعقيم .. كلمة التعقيم قلنا absolute + +536 +00:39:24,700 --> 00:39:29,400 +killing of all forms of life قد نستطيع أننا نعقمها + +537 +00:39:29,400 --> 00:39:33,380 +و نحطها في فرن على 170 درجة مئوية لمدة ساعتين، + +538 +00:39:33,380 --> 00:39:37,940 +هتعقم الإيد بس مش هتضل الإيد، لمرة واحدة، فهنشوف + +539 +00:39:37,940 --> 00:39:41,420 +الطرق المتنوعة و المختلفة و متى بنلجأ للتعقيم و + +540 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"denaturation فسخنا اللي هم المادة اللي وراتيها عن بعضها من بعض بعدين عملنا ما يسمى ب ال primer annealing تمام اللي يسميه annealing و لحظة العملية بتتم عليها على different temperatures بعد ال primer annealing اتى ال DNA polymerase وكمل اللي هو ال elongation process يعني كمل القطعة", "tokens": [1556, 267, 8167, 6156, 3794, 9778, 8315, 13672, 1829, 8032, 2304, 9673, 18513, 3660, 13672, 1829, 4032, 23557, 31371, 11296, 18871, 45030, 11242, 11296, 9154, 45030, 11242, 39182, 9957, 6225, 42213, 8315, 19446, 7251, 38251, 7578, 4724, 2423, 12595, 22256, 4270, 46811, 10943, 13672, 1829, 7251, 38251, 1829, 3224, 22256, 4270, 4032, 5296, 5016, 19913, 3660, 18863, 42213, 10632, 39894, 39237, 25894, 11296, 15844, 819, 12633, 39182, 2423, 12595, 22256, 4270, 1975, 2655, 7578, 2423, 8272, 20073, 651, 4032, 24793, 1211, 13672, 1829, 31439, 2423, 40786, 399, 1399, 37495, 22653, 9122, 42213, 25062, 9566, 27884], "avg_logprob": -0.32552083022892475, "compression_ratio": 1.7639484978540771, 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"probability": 0.794921875}, {"start": 944.7, "end": 945.0, "word": " MIC", "probability": 0.88134765625}, {"start": 945.0, "end": 947.12, "word": " كتركيز", "probability": 0.821142578125}, {"start": 947.12, "end": 950.36, "word": " ال", "probability": 0.810546875}, {"start": 950.36, "end": 950.9, "word": " MBC", "probability": 0.978515625}, {"start": 950.9, "end": 951.32, "word": " بيكون", "probability": 0.93115234375}, {"start": 951.32, "end": 951.84, "word": " تركيزه", "probability": 0.9916015625}, {"start": 951.84, "end": 952.32, "word": " أعلى", "probability": 0.9171549479166666}, {"start": 952.32, "end": 952.62, "word": " من", "probability": 0.974609375}, {"start": 952.62, "end": 952.8, "word": " ال", "probability": 0.88720703125}, {"start": 952.8, "end": 953.26, "word": " MIC", "probability": 0.95166015625}, {"start": 953.26, "end": 953.62, "word": " شوفوا", "probability": 0.7401529947916666}, {"start": 953.62, "end": 953.88, "word": " دلوقتي", "probability": 0.777099609375}, 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لذاكم", "probability": 0.631103515625}, {"start": 2425.9, "end": 2426.64, "word": " الصورة", "probability": 0.9388020833333334}, {"start": 2426.64, "end": 2426.96, "word": " لكن", "probability": 0.8564453125}, {"start": 2426.96, "end": 2427.9, "word": " الإكتئاب", "probability": 0.6783203125}, {"start": 2427.9, "end": 2428.08, "word": " من", "probability": 0.98095703125}, {"start": 2428.08, "end": 2428.32, "word": " هذه", "probability": 0.9873046875}, {"start": 2428.32, "end": 2428.82, "word": " الأدوية", "probability": 0.991455078125}, {"start": 2428.82, "end": 2429.4, "word": " تجعل", "probability": 0.9833984375}, {"start": 2429.4, "end": 2429.94, "word": " الشخص", "probability": 0.99365234375}, {"start": 2429.94, "end": 2430.68, "word": " معرض", "probability": 0.8994140625}, {"start": 2430.68, "end": 2431.56, "word": " للإدمان", "probability": 0.850341796875}, {"start": 2431.56, "end": 2432.18, "word": " مما", "probability": 0.929931640625}, {"start": 2432.18, "end": 2432.6, "word": 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tract و هو في الجهاز الهضمي بشكل أخص و هي lactobacillus acetophilus lactobacillus acetophilus كما نعلم و هي بكتيريا لبن الرائد تعد gram positive bacteria fragmented sugar and lactic acids تعيش في وسط بيئة يكون منخفض", "tokens": [2407, 34105, 4117, 8717, 45367, 9154, 14739, 14407, 3615, 2423, 4532, 24128, 44713, 9566, 34268, 14739, 6055, 5016, 1211, 1211, 5296, 8315, 5551, 24401, 5551, 35457, 16758, 29910, 23275, 9673, 43042, 4032, 31439, 3714, 46456, 9381, 8978, 2423, 7219, 1341, 24207, 4032, 31439, 8978, 25724, 3224, 31377, 2423, 3224, 11242, 2304, 1829, 4724, 8592, 28820, 5551, 9778, 9381, 4032, 39896, 34042, 996, 326, 373, 301, 37848, 5317, 388, 301, 34042, 996, 326, 373, 301, 37848, 5317, 388, 301, 9122, 15042, 8717, 3615, 19528, 4032, 39896, 4724, 4117, 2655, 13546, 25528, 5296, 3555, 1863, 34892, 16373, 3215, 6055, 22488, 21353, 3353, 11763, 9241, 14684, 5076, 293, 34042, 299, 21667, 37279, 1829, 8592, 8978, 46952, 9566, 4724, 1829, 19986, 3660, 7251, 30544, 9154, 9778, 5172, 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a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/MKQTJ5RDVMA_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/MKQTJ5RDVMA_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..0c95ead44f3b6d3d9f9b2f0d9fc4a51b00364b15 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/MKQTJ5RDVMA_raw.srt @@ -0,0 +1,2724 @@ +1 +00:00:21,510 --> 00:00:24,710 +بسم الله الرحمن الرحيم اليوم .. اليوم ان شاء الله + +2 +00:00:24,710 --> 00:00:27,970 +هنكمل الموضوع اللي اتكلمنا فيه اللي هو ال + +3 +00:00:27,970 --> 00:00:33,150 +antibiotics او ال antimicrobial تفكير بس بالعناوين + +4 +00:00:33,150 --> 00:00:37,570 +كنا بنعرف انه قسمنا ال antibacterial chemically + +5 +00:00:37,570 --> 00:00:41,030 +داخلنا فينا حاجة اسمها penicillins و macrolides و + +6 +00:00:41,030 --> 00:00:45,230 +erythromycin أفضل تفرغ سايكليز و بعدين قسمناهم + +7 +00:00:45,230 --> 00:00:48,490 +بكتيري سايبر بكتيريو aesthetic broad spectrum و + +8 +00:00:48,490 --> 00:00:52,330 +narrow spectrumو آخر تقسيمة كانت دي الأهم من على + +9 +00:00:52,330 --> 00:00:55,770 +الإطلاق اللي هي based on a side effected cell + +10 +00:00:55,770 --> 00:00:58,790 +walls, inhibitors, cell membrane, protein + +11 +00:00:58,790 --> 00:01:02,470 +synthesis, DNA, or lipid acid و آخر إش كان عندي + +12 +00:01:02,470 --> 00:01:05,230 +اللي هو ال Anti-metabolites أعتقد أن هذه اللوحة + +13 +00:01:05,230 --> 00:01:10,370 +توقفنا عنها المرة الماضية إيش يعني Anti + +14 +00:01:10,370 --> 00:01:15,010 +-metabolites؟ لو طلبنا عندي توأم، توأم متشابهين، + +15 +00:01:15,010 --> 00:01:20,620 +هل أحكيناها؟ توأم متشابهينواحد انا بعطيه مبلغ من + +16 +00:01:20,620 --> 00:01:23,880 +المال كل يوم اللي بشوفه بعطيه مبلغ من المال، هذا + +17 +00:01:23,880 --> 00:01:27,380 +الشخص بيشتري كل الأدوات اللي لازم من المنزل ولا + +18 +00:01:27,380 --> 00:01:32,640 +أخوته ولا أخواته، و الحياة بتستمر، التاني شق + +19 +00:01:32,640 --> 00:01:36,400 +التاني بالتوأم، والذي لا أستطيع تمييزه عن الأول لو + +20 +00:01:36,400 --> 00:01:41,080 +ما بقيده على حد الفلوس، ببطر على جزمه، بس هذا + +21 +00:01:41,080 --> 00:01:45,220 +الشخص التاني بياخد الفلوس و بحطهم في جبهمش هيشتري + +22 +00:01:45,220 --> 00:01:48,500 +اي حاجة من خلية و لا .. يعني الحالية إيش هتصرف .. + +23 +00:01:48,500 --> 00:01:52,900 +هتصرف .. هتجف انا اللي مابنش يميز بين اتنينهم + +24 +00:01:52,900 --> 00:01:58,120 +بيشبهوا بعض مش خدوا بالكم مش identical لكن بيشبهوا + +25 +00:01:58,120 --> 00:02:01,740 +بعض ولا سطر الإنزايم أن يميز بين ال substrate + +26 +00:02:01,740 --> 00:02:05,100 +الحقيقي و بين ال analog تبع ال analog اللي هو + +27 +00:02:05,100 --> 00:02:13,450 +الشريط أو النظيرهذه الفكرة موجودة في مركبين + +28 +00:02:13,450 --> 00:02:16,090 +مشهورين جدا في ال Antimicrobials اسمهم + +29 +00:02:16,090 --> 00:02:21,470 +Sulfamethexazol و التاني اسمه Trimethoprim كلا هما + +30 +00:02:21,470 --> 00:02:24,850 +بيقولوا بقى بالمهمة طلعوا على الصفحات التانية + +31 +00:02:24,850 --> 00:02:28,410 +الاندرجرام + +32 +00:02:28,410 --> 00:02:34,680 +اللي أمامكم هو بيوضح pathwayمسار .. مسار لتخليق + +33 +00:02:34,680 --> 00:02:37,900 +folic acid خدوا بعرفوا ال folic acid مهم جدا + +34 +00:02:37,900 --> 00:02:42,180 +لتصنيع مين؟ لتصنيع ال nucleotides و ال nucleotides + +35 +00:02:42,180 --> 00:02:47,540 +مهمة لتصنيع ال DNA و ال RNA بدون .. بدون .. بدون + +36 +00:02:47,540 --> 00:02:50,760 +folic acid اقصد لا مصدر انه وصلنا ل nucleotide ولا + +37 +00:02:50,760 --> 00:02:57,880 +حصلنا DNA و RNA طيب كيف بنوجه تصنيع ال folic acid؟ + +38 +00:02:57,880 --> 00:03:02,960 +شوفوا اول مركب اسمه بابااللي هو Fara Amino Benzoic + +39 +00:03:02,960 --> 00:03:05,440 +Acid الفارة أمينو بنزويك أسد الفارة أمينو بنزويك + +40 +00:03:05,440 --> 00:03:06,100 +أسد الفارة أمينو بنزويك أسد الفارة أمينو بنزويك + +41 +00:03:06,100 --> 00:03:06,460 +أسد الفارة أمينو بنزويك أسد الفارة أمينو بنزويك + +42 +00:03:06,460 --> 00:03:08,780 +أسد الفارة أمينو بنزويك أسد الفارة أمينو بنزويك + +43 +00:03:08,780 --> 00:03:13,160 +أسد الفارة + +44 +00:03:13,160 --> 00:03:16,980 +أمينو بنزويك أسد الفارة أمينو بنزويك أسد الفارة + +45 +00:03:16,980 --> 00:03:21,660 +أمينو بنزويك أسد الفارة + +46 +00:03:21,660 --> 00:03:26,980 +أمينو بنزويك أسد الفارة أمينو بنزويك أسد الفارة + +47 +00:03:26,980 --> 00:03:32,420 +أمينو بنزويك أسد الفارة أمينو بنزويك أسد الفمُركّب + +48 +00:03:32,420 --> 00:03:37,780 +analog لـdihydrofolic acid اللي المفروض يتحول + +49 +00:03:37,780 --> 00:03:42,280 +لـtetrahydrofolic acid يبقى لو كان فيه + +50 +00:03:42,280 --> 00:03:45,900 +dihydrofolic acid مش هيتحول لـtetrahydrofolic acid + +51 +00:03:45,900 --> 00:03:49,700 +مش هيتصنع الـfolic acid يبقى سختمة مُركّبين + +52 +00:03:49,700 --> 00:03:53,700 +كanalogues كanalogues هدول المُركّبين اشتغلوا على + +53 +00:03:53,700 --> 00:03:57,280 +خطوطين مختلفتين يعني انت بتتخيلوا مع بعض ان هو + +54 +00:03:57,280 --> 00:04:01,390 +يبقى في ويبقى في ويبقى في شغليناللي بيفلف من الأول + +55 +00:04:01,390 --> 00:04:05,970 +مافلتش من التاني فعطينا التانين مع بعض وعملناهم + +56 +00:04:05,970 --> 00:04:09,950 +مُركّب واحد بيبنيهم في السيداليات باسم واحد cell + +57 +00:04:09,950 --> 00:04:12,770 +from the hexasol تراكشحتا تراكشحتا تراكشحتا + +58 +00:04:12,770 --> 00:04:13,810 +تراكشحتا تراكشحتا تراكشحتا تراكشحتا تراكشحتا + +59 +00:04:13,810 --> 00:04:13,810 +تراكشحتا تراكشحتا تراكشحتا تراكشحتا تراكشحتا + +60 +00:04:13,810 --> 00:04:13,890 +تراكشحتا تراكشحتا تراكشحتا تراكشحتا تراكشحتا + +61 +00:04:13,890 --> 00:04:14,490 +تراكشحتا تراكشحتا تراكشحتا تراكشحتا تراكشحتا + +62 +00:04:14,490 --> 00:04:21,390 +تراكشحتا تراكشحتا تراكشحتا + +63 +00:04:21,390 --> 00:04:29,390 +تراكشحت + +64 +00:04:30,020 --> 00:04:34,840 +أما عن طريق ارتباط الأنالوج بالـ Active site بتاع + +65 +00:04:34,840 --> 00:04:36,840 +الإنزايد اللي هو Active site و اللي هو الـ + +66 +00:04:36,840 --> 00:04:40,620 +Allosteric site أو عن طريق الارتباط بالـ + +67 +00:04:40,620 --> 00:04:44,980 +Allosteric site لتغيير الـ Active site احنا اللي + +68 +00:04:44,980 --> 00:04:49,740 +بنتكلم عنه الآن هو عبارة عن ارتباط لشيء شميه بالـ + +69 +00:04:49,740 --> 00:04:51,340 +Substrate على الـ Active + +70 +00:04:54,360 --> 00:04:57,940 +بيسموه competitive inhibitors، بيسموه structural + +71 +00:04:57,940 --> 00:05:02,860 +analog، بيسموه antimicrobials، أمام؟ فهذه + +72 +00:05:02,860 --> 00:05:06,740 +الميكانيزم الخامسة اللي احنا قلنا عنها، بيقسم + +73 +00:05:06,740 --> 00:05:11,360 +البكتيريا إلى خمسة، اليوم هنتقل الصحيح إلى موضوع + +74 +00:05:11,360 --> 00:05:14,940 +ال testing لل antibiotics، وهذا أكتر موضوع مهم وده + +75 +00:05:14,940 --> 00:05:18,680 +أركز فيه لإنه في أخطاء قاتلة في مختبراتنا في هذا + +76 +00:05:18,680 --> 00:05:25,610 +البرنامجإذا أردنا أن نقوم بدراسة .. بدراسة مدى + +77 +00:05:25,610 --> 00:05:30,610 +فعالية مضاد حيوان معين على بكتيريا معينة .. بصيرش + +78 +00:05:30,610 --> 00:05:34,550 +أهالي اتنين و أعملهم مسارعة و واحد قرر بتقدر يربط + +79 +00:05:34,550 --> 00:05:38,830 +رجليه و يقول فلان غلق فلان .. بكدا بيكون في نوع من + +80 +00:05:38,830 --> 00:05:44,170 +ال biasness يعني مغوظين مع حدا .. لازم ا offer + +81 +00:05:44,170 --> 00:05:48,150 +للتانين نفس ال chances .. نفس الفرصةفبانتحار انا + +82 +00:05:48,150 --> 00:05:54,050 +اقوم بهذه المهمة عن طريق توفير وسط ملائم جدا لنمو + +83 +00:05:54,050 --> 00:05:58,730 +البكتيريا بنزرع البكتيريا بشكل طبقة وانحط اقراص من + +84 +00:05:58,730 --> 00:06:02,330 +ال antibiotics تحتوي على تركيز محدد زي ما تشايفين + +85 +00:06:02,330 --> 00:06:08,010 +هنا كل قرص من الاقراص اللي موجودة ايه الرمز وتركيز + +86 +00:06:08,510 --> 00:06:11,830 +الرمز و التركيز و الرمز يدل على نوع ال antibiotics + +87 +00:06:11,830 --> 00:06:15,450 +طبعا الرموز تختلف من شركة لشركة وبالتالي لما نمارس + +88 +00:06:15,450 --> 00:06:19,730 +المهنة تبعتنا بيكون عندنا الجدول ال antibiotics + +89 +00:06:19,730 --> 00:06:23,910 +اللي استخدمناهم من شركة معينة و هي بتعطينا اصلا ال + +90 +00:06:23,910 --> 00:06:26,390 +abbreviations اللي هي الاقتصادات ليهاش كل واحد + +91 +00:06:26,390 --> 00:06:31,110 +اقتصاد و ليهاش كل واحد او كل concentration لكل + +92 +00:06:31,110 --> 00:06:36,900 +course موجودةوضعنا الآن تقريبا على الصبورة تقريبا + +93 +00:06:36,900 --> 00:06:40,300 +على الطبق اللي حطينا فيه البكتيريا بشكل طبقة + +94 +00:06:40,300 --> 00:06:46,980 +وحطينا عليه أقراس من ال antimicrobials اتنين + +95 +00:06:46,980 --> 00:06:50,520 +حطيناهم في ال incubator وعطيناهم فرصة عطينا فرصة + +96 +00:06:50,520 --> 00:06:53,760 +لل antibiotic ان يعمل diffusion وعطينا فرصة + +97 +00:06:53,760 --> 00:06:56,980 +للبكتيريا تنمو لانونامي على وسط انا هزلها على وسط + +98 +00:06:56,980 --> 00:07:03,560 +لأنهم بروا الصورةلان انا حاول القوس في منطقة خالية + +99 +00:07:03,560 --> 00:07:07,040 +من النمو، خالية من النمو، مابعرفش killing او + +100 +00:07:07,040 --> 00:07:10,940 +inhibition، لكن هي خالية من النمو، بيسميها zone of + +101 +00:07:10,940 --> 00:07:15,360 +inhibition، مهم جدا المصطلح zone of inhibition لان + +102 +00:07:15,360 --> 00:07:17,480 +هو اللي بينجيسه، هو اللي بينجيسه + +103 +00:07:35,070 --> 00:07:39,310 +كلمة أحسن مش موجودة في قموسنا على الإطلاق صارت في + +104 +00:07:39,310 --> 00:07:40,690 +الميكروبيرجي + +105 +00:07:44,500 --> 00:07:48,960 +الدرج ان ال technician بتطلع على الطبق و بيقول هذا + +106 +00:07:48,960 --> 00:07:51,580 +زورة كبيرة، هذا sensitive، هذا زورة موسطة، هذا + +107 +00:07:51,580 --> 00:07:54,740 +intermediary، هذا زورة صغيرة، هذا resistant، هذا + +108 +00:07:54,740 --> 00:07:59,740 +الكلام أكبر جريمة ممكن يرتكبها واحد بيقدم خدمة + +109 +00:07:59,740 --> 00:08:04,320 +microbiology و دلوقت موجودة أن في القطاع و منتشرة + +110 +00:08:04,320 --> 00:08:10,700 +بينهم و يشجع حلقات أطباق بعد مواضعتهم لل protocol + +111 +00:08:10,700 --> 00:08:17,270 +المستخدمة في المختبراتطيب إيش الصح؟ قبل الصح بنسأل + +112 +00:08:17,270 --> 00:08:22,850 +السؤال، في هدا فيكم بيقدر يميز بين 16 millimeter و + +113 +00:08:22,850 --> 00:08:26,270 +17 millimeter بالعين المجردة بتاعك؟ يعني تطلع و + +114 +00:08:26,270 --> 00:08:31,400 +هيك 0.16أو يجيسها هو .. اقولك 16 و 17 ممكن نقول من + +115 +00:08:31,400 --> 00:08:34,700 +أكبر من التانية الفرق بسيط جدا لدرجة انه مابنقدرش + +116 +00:08:34,700 --> 00:08:37,580 +.. احنا بنشوفهم متساويين .. بنشوفهم متساويين في + +117 +00:08:37,580 --> 00:08:41,000 +غالب الأمر هذا أول سؤال سألته انا .. مابنقدرش احنا + +118 +00:08:41,000 --> 00:08:43,420 +بالهيئة المجردة بنميز بين أرقام قليلة من بعض + +119 +00:08:43,420 --> 00:08:47,120 +فالأصل في الأشياء القياس .. إنه نجيب مصطرة أو + +120 +00:08:47,120 --> 00:08:49,640 +caliber .. تعرفون ال caliber في هيئة الفيزيا؟ اللي + +121 +00:08:49,640 --> 00:08:55,000 +بنجيس فيه الأقطار .. بنحطه و بنجيس قطر و بنسجل .. + +122 +00:08:55,000 --> 00:08:57,580 +يبقى الزواق ال generation مش بالأعلى + +123 +00:09:00,390 --> 00:09:04,290 +أول نقطة، نقطة تانية تفسير الأرقام اللي طلعت + +124 +00:09:04,290 --> 00:09:08,400 +معانا، إيه تفسير الأرقام؟طولة معايا 17، إيش يعني + +125 +00:09:08,400 --> 00:09:11,600 +17؟ طولة معايا 20، إيش يعني 20؟ هل كل ال + +126 +00:09:11,600 --> 00:09:15,000 +antibiotics أرقامهم واحد بتفسيراتهم؟ لأ، أنا بتقول + +127 +00:09:15,000 --> 00:09:18,260 +فيها lista قائمة بأسماء ال antibiotics بال + +128 +00:09:18,260 --> 00:09:22,400 +abbreviation وفي تلت أعمدة كمان، إيش الأعمدة دي؟ + +129 +00:09:22,400 --> 00:09:28,260 +RIS، R يعني resistant، I intermediate، S يعني + +130 +00:09:28,260 --> 00:09:32,190 +sensitive أو susceptible، نفس المعنىSusceptible + +131 +00:09:32,190 --> 00:09:35,010 +لأنه حتى بيقول Antibiotic Sensitivity أو + +132 +00:09:35,010 --> 00:09:40,530 +Antibiotic Susceptibility نفس المعنى أكم عمود صار؟ + +133 +00:09:40,530 --> 00:09:44,710 +طب Antibiotic Abbreviation Disk Potency أو + +134 +00:09:44,710 --> 00:09:50,570 +Concentration RIS إيش أن R بيبقى resistant؟ بيكون + +135 +00:09:50,570 --> 00:09:59,490 +فيه رقم إذا أقل من فهي R إذا بين X و Y فهي Iإذا + +136 +00:09:59,490 --> 00:10:04,590 +كان أكتر من أو يساوي فهي S يبقى في الآخر آية بدي + +137 +00:10:04,590 --> 00:10:10,590 +أجيس والرقم اللي بيطلع أطبّه على مين على ترجمة و + +138 +00:10:10,590 --> 00:10:16,430 +أشوف هل هو R ولا I ولا S طب أعطيكوا أمثلة مش دقيقة + +139 +00:10:16,430 --> 00:10:23,180 +لكن بتعبّل على الفكرة تبقى تعرف بانسلين؟و في حدث + +140 +00:10:23,180 --> 00:10:25,860 +ما جنتا ويسيني جنتا ويسيني مرة بعد محلقة السابقة + +141 +00:10:25,860 --> 00:10:28,700 +في ال amino glycoside و البنسريب هو ال superstar + +142 +00:10:28,700 --> 00:10:35,780 +أول أنت بدي أصلا أن أعرف Staphylococcus aureus إذا + +143 +00:10:35,780 --> 00:10:43,120 +كان الرقم 25 millimeter في البنسريب بيكون + +144 +00:10:43,120 --> 00:10:48,810 +resistantبالإضافة لو كان رقم 17 في ال gene بميسين + +145 +00:10:48,810 --> 00:10:53,870 +بتكون sensitive، مين أكبر؟ ال 25 و 17 و اعتقد ان + +146 +00:10:53,870 --> 00:10:59,810 +علامة بتميز بين 25 و 17 و هذا أكبر من تاني و مع + +147 +00:10:59,810 --> 00:11:03,810 +ذلك مين ال sensitive كانت؟ ال 17 كانت sensitive و + +148 +00:11:03,810 --> 00:11:07,750 +ال 25 كانت resistant، إذا ال zone مرتبطة بنوع ال + +149 +00:11:07,750 --> 00:11:13,090 +antibiotic و قد تكون مرتبطة بنوع البكتيري أيضاإذا + +150 +00:11:13,090 --> 00:11:17,390 +بنفهم من القيامة إنه لا قيادة .. لا .. لا فتوة لا + +151 +00:11:17,390 --> 00:11:23,090 +إصدار نتائج based on visual inspection ولا جداول + +152 +00:11:23,090 --> 00:11:29,070 +مبنية على اللي هو القطر دولة رجوها إلى الجداول اسم + +153 +00:11:29,070 --> 00:11:33,110 +الطريقة هذه disk diffusion method ليش سموها disk + +154 +00:11:33,110 --> 00:11:36,450 +diffusion method؟ لأنه ستفتح من أقراس ولأن لو نحط + +155 +00:11:36,450 --> 00:11:41,390 +الأقراس احنا ال diffusion تبعها بشكل باطل ده ايضا + +156 +00:11:41,390 --> 00:11:48,040 +disk diffusionبدأنا اختراع قراهيب اتطلع عليه اسمه + +157 +00:11:48,040 --> 00:11:51,680 +E-test ممكن نصبعه على شويه و بعدين نرجعله نصبعه + +158 +00:11:51,680 --> 00:11:55,480 +على شويه و بعدين نرجعله نشوف حاجة تانية اسمها MIC + +159 +00:11:55,480 --> 00:12:00,520 +و MBC ال MBC يعني مش القناة ال MBC و انما هي اللي + +160 +00:12:00,520 --> 00:12:06,660 +هي Minimum Bacterial Cycle Concentration برضه + +161 +00:12:06,660 --> 00:12:10,080 +ركزوا معايا الأهال تركيز جيد عشان بنفهم ايش يعني + +162 +00:12:10,080 --> 00:12:15,000 +MIC و ايش يعني MBCفكيف نقول ان ال antibiotic ممكن + +163 +00:12:15,000 --> 00:12:17,900 +يكون بكتيري سايدل او ممكن يكون بكتيري سايدل؟ وإذا + +164 +00:12:17,900 --> 00:12:22,080 +بتتذكر المحاضرة ارجعولها هتلاقوها لقول جملة إن + +165 +00:12:22,080 --> 00:12:25,240 +static أو سايدل is a matter of concentration إذا + +166 +00:12:25,240 --> 00:12:29,140 +اختفت عن ال concentration هو سايدل لكن قررنا إنه + +167 +00:12:29,140 --> 00:12:31,400 +سايدل ال antibiotic أو الstatic based on the + +168 +00:12:31,400 --> 00:12:36,060 +therapeutic dose على الجرع الألوجي هل هو static + +169 +00:12:36,060 --> 00:12:41,020 +ولا سايدل؟هل من الممكن أننا في المقتبرة نحدد ال + +170 +00:12:41,020 --> 00:12:44,800 +MIC و ال MBC نعدي لحدها بكل سهولة و يسر بالطريقة + +171 +00:12:44,800 --> 00:12:48,100 +اللي قدامكم اللي بتقول أن في عندي مجموعة من + +172 +00:12:48,100 --> 00:12:51,860 +القنابيب شايفينها؟ مجموعة من القنابيب من واحد + +173 +00:12:51,860 --> 00:12:55,100 +لجداشها تسعة والاخيرة control control يعني بنحط + +174 +00:12:55,100 --> 00:12:59,080 +فيها بكتيريا عشان نضمن ان .. مابحثش انتباهك عشان + +175 +00:12:59,080 --> 00:13:03,280 +نضمن ان البكتيريا نمر في عاملة turbidityالقنابيب + +176 +00:13:03,280 --> 00:13:08,160 +حطينا من واحد على تركيز نصه فى اتنين و نصه فى + +177 +00:13:08,160 --> 00:13:12,080 +تلاتة اشهر نصه يعنى اعملنا CGAPUSA يعنى لو كان + +178 +00:13:12,080 --> 00:13:16,980 +واحد جرعه نص جرعه رده جرعه و احد كده لما نص اللا + +179 +00:13:16,980 --> 00:13:21,080 +الاخر بعدين حطينا على القنابيب كلياتهم نفس ال + +180 +00:13:21,080 --> 00:13:25,160 +equal volume من البكتيريا تمام؟ و حطيناهم فى ال + +181 +00:13:25,160 --> 00:13:29,020 +incubator و قولنا للبكتيريا و ال antibiotic مين + +182 +00:13:29,020 --> 00:13:33,860 +يغلببعد 24 ساعة طلعناهم من ال incubator اللي جينا + +183 +00:13:33,860 --> 00:13:39,040 +رقم واحد مش فيها نمو طلع الأخيرة ال control فيها + +184 +00:13:39,040 --> 00:13:41,660 +نمو و واره ان فيها نمو بينما الأولى مافيهاش نمو و + +185 +00:13:41,660 --> 00:13:44,820 +التانية و التالتة و الرابعة و الخمسة بينما بدأ + +186 +00:13:44,820 --> 00:13:50,700 +النمو يظهر في السادسة طيب تعريف ال MIC بيقول the + +187 +00:13:50,700 --> 00:13:53,900 +lowest concentration of antibiotics that inhibited + +188 +00:13:53,900 --> 00:13:56,060 +the growth of microorganisms + +189 +00:13:58,310 --> 00:14:01,910 +وين ال lowest concentration اللي عملت inhibition + +190 +00:14:01,910 --> 00:14:07,110 +لل growth of microorganism في الصدورة؟ خمسة، ستة + +191 +00:14:07,110 --> 00:14:12,370 +فيها نمو، هي اللي جابتها هي ال lowest + +192 +00:14:12,370 --> 00:14:17,450 +concentration اللي عملت، مصبوط او لأ؟أقل التركيز، + +193 +00:14:17,450 --> 00:14:19,910 +أقل الانهيبوش اللي هو الأنبوبة الخامس، طب إذا أنا + +194 +00:14:19,910 --> 00:14:21,390 +عارف التركيز اللي هو الأنبوبة الأولى، كده عارف + +195 +00:14:21,390 --> 00:14:24,010 +التركيز اللي هو الأنبوبة الخامس طبعا عاملة cereal + +196 +00:14:24,010 --> 00:14:28,510 +dilution، يبقى أختارنا ال MIC بناء على the lowest + +197 +00:14:28,510 --> 00:14:30,430 +concentration that inhibited the growth of + +198 +00:14:30,430 --> 00:14:33,170 +microorganisms أو test organisms، ميش الأقل ال + +199 +00:14:33,170 --> 00:14:38,930 +NBC؟احنا الآن اللي صار مش عارفين هل هو قتل أصلا + +200 +00:14:38,930 --> 00:14:43,290 +ولا .. ولا anything فبنعمل sub culture بس خدوا + +201 +00:14:43,290 --> 00:14:45,790 +بالك الصورة اللي عندي هنا مجلوبة شوية بلال ما هي + +202 +00:14:45,790 --> 00:14:51,330 +بدأ من واحد من هنا لو واحد بدأ من هنا فمثال عندي + +203 +00:14:51,330 --> 00:14:56,030 +تركيز هنا تمانية وهنا أربع وهنا اتنين وهنا واحد + +204 +00:14:56,030 --> 00:15:00,660 +milligram الأخيرة مصم milligram شايفينها؟في الخمس + +205 +00:15:00,660 --> 00:15:04,560 +قنابيب الأخيرة اللي هي نص جرام نص جرام عاملة + +206 +00:15:04,560 --> 00:15:10,660 +تربيتي عاملة تربيتي بجميع ال MIC اللي جابلها صح؟ + +207 +00:15:10,660 --> 00:15:14,060 +مش كنت أخر واحدة عاملة تربيتي اللي جابلها هي ال + +208 +00:15:14,060 --> 00:15:18,160 +MIC روحنا أخدنا من كل القنابيب اللي صار فيهم + +209 +00:15:18,160 --> 00:15:21,800 +inhibition loop و زرعناها على دلوقت أجار أو على + +210 +00:15:21,800 --> 00:15:24,600 +رترد أجار أو على ملارمتهم حسب الوضع أو حسب + +211 +00:15:24,600 --> 00:15:29,320 +البكتيريا لحظوا اللي هو النمو صار هنا + +212 +00:15:39,560 --> 00:15:44,620 +يبقى مين أكتر بيكون في القيمة في الرقم ال MBC و لا + +213 +00:15:44,620 --> 00:15:50,360 +ال MIC كتركيز ال + +214 +00:15:50,360 --> 00:15:54,980 +MBC بيكون تركيزه أعلى من ال MIC شوفوا دلوقتي قاعدة + +215 +00:15:54,980 --> 00:16:01,680 +يستحيل أن ال MBC تكون أقل من ال MIC أقل عشان هذا + +216 +00:16:01,680 --> 00:16:04,940 +الكلام أقل concentration أقل concentration كلام + +217 +00:16:04,940 --> 00:16:07,940 +يعني مفهوم ولا مش مفهوم لسه انهيض .. انهيض .. + +218 +00:16:07,940 --> 00:16:15,350 +انهيضه كمان مرةالان ال inhibition المفروض بيكون + +219 +00:16:15,350 --> 00:16:20,830 +تركيز لو زدت و انا ممكن أصير killing ال killing لو + +220 +00:16:20,830 --> 00:16:23,850 +مجز ممكن أصير inhibition يبقى باله ان ال + +221 +00:16:23,850 --> 00:16:28,930 +inhibition اقل من ال killing اقل في التركيز اقل في + +222 +00:16:28,930 --> 00:16:32,670 +التركيز فعشان انا احصل على القتل احصل على القتل + +223 +00:16:32,670 --> 00:16:37,470 +بدي اللي هو تركيز ايه اعلى فبيعمل ال MBC يا بساوي + +224 +00:16:37,470 --> 00:16:42,410 +ال MIC يا اعلى منهاهيكون الصورة واضحة طبعا بقيت + +225 +00:16:42,410 --> 00:16:47,890 +انا عملتها كده وقفزت عن صورة بدرجة أعلى اللي هي E + +226 +00:16:47,890 --> 00:16:55,650 +-test ال E-test اختراع عطولة strip ال + +227 +00:16:55,650 --> 00:16:59,690 +concentration فيه gradient ايش يعني gradient من + +228 +00:16:59,690 --> 00:17:03,130 +أعلى إلى أسفل طبعا تقنية خدوا باركم السهل عملها + +229 +00:17:03,130 --> 00:17:07,090 +strip بلاستيك فبالتالي تقنية يبدو انها patent يعني + +230 +00:17:07,090 --> 00:17:11,820 +محصولة براد اختراع ف expensiveيعني م .. م .. + +231 +00:17:11,820 --> 00:17:15,040 +موفودش لازم تقنية إلا مرة واحدة لدراسة، أعملنا + +232 +00:17:15,040 --> 00:17:20,660 +دراسة فيها، تمام؟ فمش common، لكن الفكرة فيها إنه + +233 +00:17:20,660 --> 00:17:25,760 +تركيز مختلفة من Antibiotic 1 أنحط على طبق خمسة، + +234 +00:17:25,760 --> 00:17:29,760 +خمس أنواع من ال antibiotic انفحصوا في طبق واحد، بس + +235 +00:17:29,760 --> 00:17:32,760 +هذا أربع، واحد antibiotic + +236 +00:17:37,520 --> 00:17:43,000 +يبقى العملية هنا مضنية ومتهبة و بتاخد وقت و بتاخد + +237 +00:17:43,000 --> 00:17:48,560 +space فمن المقارنة مين أحسن؟ ال a test طب ال a + +238 +00:17:48,560 --> 00:17:51,460 +test مين أحسن كان مرة اللي وجهنا نفس الغلطة ال a + +239 +00:17:51,460 --> 00:17:58,400 +test جيد لكن expensive هذه رخيصة لكن متهبة يبقى كل + +240 +00:17:58,400 --> 00:18:01,860 +واحدة اللي كانت إيجابية أو سلبية تعالوا ناخد حل + +241 +00:18:01,860 --> 00:18:02,220 +وسط + +242 +00:18:06,620 --> 00:18:12,000 +الصورة اللى قدامنا انصر + +243 +00:18:12,000 --> 00:18:15,740 +الجزر اللى مكتوب كتابها نطلع على ال micro title + +244 +00:18:15,740 --> 00:18:18,740 +plate ال micro title plate هو عبارة عن plate + +245 +00:18:18,740 --> 00:18:24,980 +بيحتوي على 96 wells 96 تمانية في 12 عارفين تمانية + +246 +00:18:24,980 --> 00:18:30,500 +اللى هم العمودي و 12 تعالى نبدأ من الجهة اليمنى في + +247 +00:18:30,500 --> 00:18:37,980 +الأطباق ناخد هذهتركيز الانتيبوتك هنا نفترض انه + +248 +00:18:37,980 --> 00:18:42,980 +واحد نفترض انه واحد خففنا ايش سنة بعدين بيكون؟ + +249 +00:18:42,980 --> 00:18:49,060 +مصر، 125، 112 وربع .. إلى آخر اللي عند وين؟ اللي + +250 +00:18:49,060 --> 00:18:53,580 +عند آخر وين؟ انتيبوتك اتنين، هذا انتيبوتك واحد، + +251 +00:18:53,580 --> 00:18:56,140 +هذا اتنين، هاي تلاتة، هاي اربعة، خمسة، ستة، سبعة + +252 +00:18:56,140 --> 00:18:59,420 +الأولاني عملناها ك control كما تلاحظوا هنا فيها + +253 +00:18:59,420 --> 00:19:06,120 +بوستر graph control فممكن افحص فيه اناأو أبحث + +254 +00:19:06,120 --> 00:19:10,700 +تمانية وممكن أبحث أتناشر إذا أنا كنت عندي معارفة + +255 +00:19:10,700 --> 00:19:15,980 +مسبقة بالتركيز المتوقع قبل ما أبدأ التركيز من + +256 +00:19:15,980 --> 00:19:21,680 +العدد الأتناشر فانزل من واحد ونزل وانزل كعمدة عمل + +257 +00:19:21,680 --> 00:19:25,720 +تخفيف بشكل عمودي و لا بشكل أفقي فهك بدي تعرف + +258 +00:19:25,720 --> 00:19:29,260 +الأنواع من ال antibiotic بس بقلل مين التخفيفة بقلل + +259 +00:19:29,260 --> 00:19:37,280 +التخفيفة عمل تخفيف لل antibioticX وY وZ اللي هم + +260 +00:19:37,280 --> 00:19:42,100 +بالعدد السابع هدول حطيت عليهم نفس البكتيريا نفس + +261 +00:19:42,100 --> 00:19:44,720 +البكتيريا Staphylococcus aureus على سبيل المثال + +262 +00:19:44,720 --> 00:19:50,700 +الآن كلهم بيحتووا على نفس العدد من البكتيريا الشيء + +263 +00:19:50,700 --> 00:19:55,060 +المختلف هو ال antibiotic و تراكيزه ال antibiotic و + +264 +00:19:55,060 --> 00:19:58,020 +تراكيزه أخدت ال microheater plate ودده على ال + +265 +00:19:58,020 --> 00:20:02,540 +incubator 24 ساعات طلعت مشكلتي فيها اللي بنعمل + +266 +00:20:02,540 --> 00:20:06,830 +فحصات أن ال turbidity مش واضحةيعني انا ممكن اقول + +267 +00:20:06,830 --> 00:20:11,890 +turban مش turban و الاختراف مع بعض على ان مين ال + +268 +00:20:11,890 --> 00:20:14,690 +turban و كلمة turban في الحلقة بتعرفين هي بتحدد من + +269 +00:20:14,690 --> 00:20:19,990 +ان في نمو و مش هي نمو ففي اختراف عزيز بنضيفه على + +270 +00:20:19,990 --> 00:20:24,290 +ال media اللي هو تترازوليوم الكلورايد تترازوليوم + +271 +00:20:24,290 --> 00:20:32,150 +كلورايد من ورا عرفته عادة في ال motility test نفس + +272 +00:20:32,150 --> 00:20:36,400 +المراكب اللي سمناها في ال motility testطيب وضعناها + +273 +00:20:36,400 --> 00:20:41,880 +بعد ال incubation بكميات محددة على كل الأطفال إذا + +274 +00:20:41,880 --> 00:20:45,620 +في بكتيريا بناك بصير اللون أحمر إذا مافيش بكتيريا + +275 +00:20:45,620 --> 00:20:49,480 +بيظل اللو كالس لأ طبعا ال .. ال .. ال .. ال .. + +276 +00:20:49,480 --> 00:20:53,600 +المادة المضافة كانت زرقاء أو تبروها فاضية مافيش + +277 +00:20:53,600 --> 00:20:59,170 +لون وين بيتحول اللون؟بعدها دي صح اللي جالك تتقولها + +278 +00:20:59,170 --> 00:21:02,090 +ايه MIC ايه ال MIC؟ ايه ال MIC؟ ايه ال MIC؟ و انا + +279 +00:21:02,090 --> 00:21:07,070 +عامل X هي طلع ال MIC وين؟ هنا، لهنا، من ضمنك، و + +280 +00:21:07,070 --> 00:21:12,410 +أول و أحسن، بأول و أحسن، حكي انه طيب السؤال كلمة + +281 +00:21:12,410 --> 00:21:16,890 +مين أحسن؟ مين أحسن؟ لإن المرة قعدت مين أحسن قعدت + +282 +00:21:16,890 --> 00:21:22,770 +مين أحسن، مش فيها خبعة أنت بيتك ال MIC دبعته كبيرة + +283 +00:21:22,770 --> 00:21:28,560 +في الرقم ولا صغيرة؟كل ما ذكر ال MIC تبقى يعني ايه؟ + +284 +00:21:28,560 --> 00:21:32,340 +ال effectiveness صحيح؟ ال effectiveness فكل اللي + +285 +00:21:32,340 --> 00:21:35,800 +بيصوره يعني الملعقة اللي لو لقيتلي أنت و بقيتك + +286 +00:21:35,800 --> 00:21:41,160 +تلعب عدّى كل هذه اللي بيبقى ال style colorless + +287 +00:21:41,160 --> 00:21:45,800 +بيكون ممتاز مانا أحكي إن ال MIC تبقى صغيرة جدا + +288 +00:21:45,800 --> 00:21:51,960 +طبعا إيه اسم ال growth dilution؟ وهو اسم الموضوعبس + +289 +00:21:51,960 --> 00:21:55,320 +عشان .. عشان اشتغلناها في wells صغيرة بيسموها + +290 +00:21:55,320 --> 00:21:59,620 +micro growth dilution micro growth dilution ايه + +291 +00:21:59,620 --> 00:22:02,360 +كان الطريقة اللي قصدناها فيها الأهل؟ عرفنا disk + +292 +00:22:02,360 --> 00:22:04,700 +diffusion واخدوا بالك ال disk diffusion هذي هي + +293 +00:22:04,700 --> 00:22:06,660 +الأهم على الإطلاق لكوا في التحيات الطبي يعني ان هي + +294 +00:22:06,660 --> 00:22:09,360 +اللي بتشتغلوا فيها ال sensitivity testing ال + +295 +00:22:09,360 --> 00:22:13,000 +sensitivity testing وانا صحي بحب استغلها و اوسع + +296 +00:22:13,000 --> 00:22:17,840 +منها لك الطلاب في كيفية استخدام او استغلال هذه + +297 +00:22:17,840 --> 00:22:18,200 +الطريقة + +298 +00:22:21,540 --> 00:22:25,700 +و جيبولنا سائل + +299 +00:22:25,700 --> 00:22:31,800 +مطهر و جيبولنا بنشوف الفعالية تبعتهممكن أستخدم نفس + +300 +00:22:31,800 --> 00:22:35,380 +الطريقة بدون antibiotic discs بس للأسف مش هيعطوني + +301 +00:22:35,380 --> 00:22:40,800 +هم دسكات فيها المطهر ولا القيمة هم هيعطوني مطهر + +302 +00:22:40,800 --> 00:22:44,600 +سائل صح ولا لأ، إيش ممكن أعمل أنا؟ يا بكمل أندي + +303 +00:22:44,600 --> 00:22:49,620 +جلتر بيرر ديسك معقمين و بدمسهم بكمية محددة و بحطهم + +304 +00:22:49,620 --> 00:22:53,900 +زي ما أنا حاطب دسكات دولار أو بطريقة تانيةاحنا + +305 +00:22:53,900 --> 00:22:57,200 +بصرا على البكتيريا بطبقها، بتعرف ال tip تبعت ال .. + +306 +00:22:57,200 --> 00:23:00,140 +ال .. ال yellow tips و ال blue tips، بنجيب ال blue + +307 +00:23:00,140 --> 00:23:05,220 +tip و بنفقه بالطبق خمس أو ست موادة و بننيدل .. + +308 +00:23:05,220 --> 00:23:10,000 +بنفرغ ال .. الأجار لما نبقى بصير فيه حفر، حفر، + +309 +00:23:10,000 --> 00:23:14,720 +اسمه well .. well diffusion، بلغ مدرسك صارش لقاعد + +310 +00:23:14,720 --> 00:23:19,700 +حفرة well diffusionبضيف فيها الكمية المطلوبة للفحص + +311 +00:23:19,700 --> 00:23:22,740 +و بضيعها من خلالها برضه و كثير من زميلات كهوئي + +312 +00:23:22,740 --> 00:23:25,480 +اللي عملوا دراسات على antimicrobial activity لبعض + +313 +00:23:25,480 --> 00:23:28,800 +النباتات او مشتقات النباتات عملوا بهذه الطريقة و + +314 +00:23:28,800 --> 00:23:32,400 +حصلوا نتائج ممتازة جدا اذا .. ببنقدر من خلال .. + +315 +00:23:32,400 --> 00:23:35,600 +طبعا ال .. ال .. عملية ال antimicrobial testing + +316 +00:23:35,600 --> 00:23:43,090 +مهمة جدا اعطيكوا مثال واحد اعطينا antibioticبنشوف + +317 +00:23:43,090 --> 00:23:47,210 +ال antibiotic وصل فى دسمه بتركيز كافى لقتل + +318 +00:23:47,210 --> 00:23:50,130 +البكتيريا اللى أخدنا منه عينة دم فصلناها السيرام، + +319 +00:23:50,130 --> 00:23:53,350 +الآن المفروض أن ال antibiotic هو السيرام، بياخد + +320 +00:23:53,350 --> 00:23:58,380 +السيرام بعد report testing بالطريقة هذهإمكانية + +321 +00:23:58,380 --> 00:24:02,860 +توظيف هذه الفكرة عالية جدا في مجالات متنمو لكن في + +322 +00:24:02,860 --> 00:24:06,380 +الآخر كلها تتلخص باني اشوف مدى antimicrobial + +323 +00:24:06,380 --> 00:24:09,820 +activity لمركب على جزء اكبر من الشغل اللي نشتغله + +324 +00:24:09,820 --> 00:24:12,620 +بالكيميا مع الكيميا هو اللي ناخد مركبته و نبحث عنه + +325 +00:24:12,620 --> 00:24:15,960 +معاهم بالطريقة هذه اللي جينا في zone of inhibition + +326 +00:24:15,960 --> 00:24:20,480 +بنعمل ال micro process dilution + +327 +00:24:27,470 --> 00:24:31,150 +دى جزء التانى من الموضوع المتعلق بالـ testing اللى + +328 +00:24:31,150 --> 00:24:34,230 +هو ال resistance ال resistance وانا بقى بحملكوا + +329 +00:24:34,230 --> 00:24:37,150 +أمانة يعنى أمانة إنه المعلومات اللى بناخدها اليوم + +330 +00:24:37,150 --> 00:24:41,550 +إنها بنشرها فيها بالناس في المجتمع لإنه إلها أهمية + +331 +00:24:41,550 --> 00:24:47,310 +كبيرة في يعني المحاولة تخفض هذه الفائرة في مجتمع + +332 +00:24:47,310 --> 00:24:54,830 +الفلسطين أي تغيير في ال DNA تبع البكتيريا بنسميه + +333 +00:24:54,830 --> 00:24:56,650 +mutation وغالباً ال resistance + +334 +00:24:59,760 --> 00:25:05,820 +أو إنها Induced، Induced يعني بفعل بفعل، Mechanism + +335 +00:25:05,820 --> 00:25:10,960 +of Antibiotic Resistance، الآن مختلفة واضحة، نتكلم + +336 +00:25:10,960 --> 00:25:15,960 +عن أن الفكرة العامة أنه mutation، لكن كيف بتصير؟ + +337 +00:25:15,960 --> 00:25:19,720 +بكتيريا عندها أنزيم قادر على تكسير، قادر على تكسير + +338 +00:25:19,720 --> 00:25:21,260 +اللي هو ال Antibiotic، + +339 +00:25:25,520 --> 00:25:28,940 +هو تتكلم عن نموذج لو بدنا ندخل في تقاط أصيل كل + +340 +00:25:28,940 --> 00:25:32,620 +الانتيبايتكس يلو ميكانيزم حتى في تباون إنزمات + +341 +00:25:32,620 --> 00:25:36,600 +مختلفة enzymatic destruction of the drug واخدنا + +342 +00:25:36,600 --> 00:25:39,820 +مثلها البرنامج الاساسي preventing of penetrating + +343 +00:25:39,820 --> 00:25:43,860 +of drug يعني ال drug بده يدخل ويعدى لأن حجمه صغير + +344 +00:25:43,860 --> 00:25:47,080 +بكل سهولة و سلاسة و كانت ال protein بيقول اتفضل + +345 +00:25:47,080 --> 00:25:50,800 +ودخلوا جوه لو صار في mutationفي ال target اللي + +346 +00:25:50,800 --> 00:25:54,720 +براني تبع ال entrance مش هدفه دلوقتي ودلوقتش هو ال + +347 +00:25:54,720 --> 00:25:58,960 +antibiotic useless تالتا انه يغير من ال target + +348 +00:25:58,960 --> 00:26:02,940 +صحيح قلتلكوا انا بيرتبط بال 50S لما يرتبط بال 50S + +349 +00:26:02,940 --> 00:26:06,540 +بيمنع ال translocation فيقول بيرتبط ال 50S ما هو + +350 +00:26:06,540 --> 00:26:10,940 +له target للارتباط إذا البكتيريا عملت mutation في + +351 +00:26:10,940 --> 00:26:14,620 +هذا ال target خدوا بركوا mutation في target لم + +352 +00:26:14,620 --> 00:26:19,220 +يؤثر على الوظيفةيعني أكرد الباب ده اللي أكرد باب، + +353 +00:26:19,220 --> 00:26:22,980 +تمام؟ لكن الاختلاف إن دياجك بطلت تسمع للـ + +354 +00:26:22,980 --> 00:26:26,220 +antibiotic إنه يبقى بطفي، هذا الكلب حاكيناه قبلين، + +355 +00:26:26,220 --> 00:26:29,980 +الراب عالمي زي كتير اسمها rapid ejection of the + +356 +00:26:29,980 --> 00:26:33,120 +tractor، مش هنفهم اللي مابيقنها؟ فضلوا يابانها، مع + +357 +00:26:33,120 --> 00:26:37,440 +السلامةبتاخدوا الدب من الباب هذا، تمام؟ و بيطلعوا + +358 +00:26:37,440 --> 00:26:40,200 +من الباب تاني و كأنه صار فيه انديو transport + +359 +00:26:40,200 --> 00:26:44,840 +system انها بيسموه rapid bombing او rapid ejection + +360 +00:26:44,840 --> 00:26:48,340 +efflux bomb بيسموها efflux يعني اللي هي الدخل + +361 +00:26:48,340 --> 00:26:53,320 +للخارج، efflux bomb الأهم اننا نسترجع المعلومات + +362 +00:26:53,320 --> 00:26:56,340 +تبعتنا اللي كانت كلها معاها ال gene transfer خدوا + +363 +00:26:56,340 --> 00:27:01,240 +بالكم الأم تنقل لبنتها للجينات، إيش بنسمي هذا نقل + +364 +00:27:01,240 --> 00:27:02,420 +من الجينات؟ + +365 +00:27:16,120 --> 00:27:18,560 +انتقلت للتنتال + +366 +00:27:24,910 --> 00:27:27,230 +للـ horizontal gene transfer اللي درسته انتوا + +367 +00:27:27,230 --> 00:27:31,530 +تلاتة ايش هما؟ conjugation، transformation و + +368 +00:27:31,530 --> 00:27:36,170 +transduction هدولة أهم تلات علاصر لنقل ال + +369 +00:27:36,170 --> 00:27:39,150 +resistance من بكتيريا إلى بكتيريا أخرى، فهذه نقطة + +370 +00:27:39,150 --> 00:27:43,070 +كتير مهمة، الآن يجب أن نسترجعها و نتذكر كيف تنتقل + +371 +00:27:43,070 --> 00:27:49,430 +هذه الأشياء طيب كيف تنشأ بعض ال resistance؟ انا + +372 +00:27:49,430 --> 00:27:51,890 +ممكن مرأي في واحد بشكل مبسط و قلت لو في على + +373 +00:27:51,890 --> 00:27:56,760 +الطاولة عندي مليون خليةكلهم sensitive للبنسالين + +374 +00:27:56,760 --> 00:28:00,960 +إلا واحد هذه الواحدة المسكينة .. مسكينة بين + +375 +00:28:00,960 --> 00:28:04,680 +المليارة أو المليون هذا المنافسة التي بعتها على + +376 +00:28:04,680 --> 00:28:11,240 +الغذاء مش سهلة ويمكن أن تموت قبل البكتيريا الحساسة + +377 +00:28:11,240 --> 00:28:17,320 +لكن لو أنا استخدمتالبنسالين فانا اللي اخترتها + +378 +00:28:17,320 --> 00:28:22,640 +باستخدام البنسالين مواتت كل إشي و خلتها هي و كوني + +379 +00:28:22,640 --> 00:28:26,740 +خلتها هي تمام فعطيت المساحة اللي جاب محتلها + +380 +00:28:26,740 --> 00:28:30,720 +المليارة و المليون و الإضاءة و ال environment كلها + +381 +00:28:30,720 --> 00:28:31,440 +لهذا البكتيري + +382 +00:28:43,900 --> 00:28:48,180 +مش هنقول إن أصلا الفطرية بتنتج antibiotics و + +383 +00:28:48,180 --> 00:28:52,220 +البكتيريا بتنتج antibiotics لكن كانت تحدث بمعدلات + +384 +00:28:52,220 --> 00:28:56,020 +ضائلة جدا جدا لما كانت العملية natural لما صار + +385 +00:28:56,020 --> 00:28:59,780 +الإنسان .. و تذكروا .. الإنسان بيستعملون قطف + +386 +00:28:59,780 --> 00:29:03,180 +الثمار في المعارفات الأخرى محدد ما عرفنا الظاهر أن + +387 +00:29:03,180 --> 00:29:06,160 +ال antibiotic أخذناهم و بقلنا نستخدمهم بشكل كبير + +388 +00:29:06,160 --> 00:29:11,480 +اسمعوا بعد مبادر .. مبادر سوء استخدام الموضوعات + +389 +00:29:11,480 --> 00:29:15,940 +الحيويةو جيسوه على مجتمع ان هل الموضوع اللي موجودة + +390 +00:29:15,940 --> 00:29:18,720 +عندكوا في البرموجودة اللي موجودة؟ انا قعدت كده و + +391 +00:29:18,720 --> 00:29:22,180 +درست في كليه كتب أذن سنوات ل media global و كنت + +392 +00:29:22,180 --> 00:29:27,500 +أسالهم السؤال مين طبيب المنزل؟ طبيب المنزل يعني + +393 +00:29:27,500 --> 00:29:31,540 +مان أخر، مين اللي بيكون بالمهمة يعالج الأطفال؟ فيه + +394 +00:29:31,540 --> 00:29:35,680 +طبعا أبسط ناس .. أبسط ناس اللي .. اللي مابي المنزل + +395 +00:29:35,680 --> 00:29:40,560 +اللي بيجي على الدكتورة، هذا أمتاز، perfectالانصاب + +396 +00:29:40,560 --> 00:29:46,000 +المتعلمين يعني اما مدرسة اما مدرسة فبقى و بتقول + +397 +00:29:46,000 --> 00:29:50,120 +الطالب الطالب الطب الآن يعني صادقة في وصفها بتقول + +398 +00:29:50,120 --> 00:29:53,960 +امي طول عمرها اللي بتشترينا الأدوية و اللي بتودينا + +399 +00:29:53,960 --> 00:29:56,860 +او ديونا من الصيدلي بتروح الصيدلي بتقوله هات + +400 +00:29:56,860 --> 00:30:02,560 +اعطيني ده للوازي اعطيني ده و انا كده هذا اسمه + +401 +00:30:02,560 --> 00:30:05,960 +misuse ل .. ال .. ال .. ال .. ال .. + +402 +00:30:11,680 --> 00:30:14,720 +using outdated .. outdated دي يعني إبتدالية تبعها + +403 +00:30:14,720 --> 00:30:19,220 +منتهي weakened antibiotics طبعا لما بنتهي صلاحيتها + +404 +00:30:19,220 --> 00:30:26,110 +أو قساء تخزينها ال antibiotic تضعفوبالتالي بناخدها + +405 +00:30:26,110 --> 00:30:30,430 +احنا هي بناخدها ل 150 ملي جرام عصير المثال او من + +406 +00:30:30,430 --> 00:30:34,050 +500 و اقع نعمل active compound منها لان بكتر واحد + +407 +00:30:34,050 --> 00:30:39,810 +من 100 فبالتالي هتبعرض البكتيريا لثرائيز منخفضة + +408 +00:30:39,810 --> 00:30:43,650 +فإن كانت ال resistance مش لان اعلى using + +409 +00:30:43,650 --> 00:30:46,410 +antibiotics for the common cold and other + +410 +00:30:46,410 --> 00:30:47,010 +inappropriate + +411 +00:30:49,920 --> 00:30:54,180 +Does not require antibiotic treatment. They are + +412 +00:30:54,180 --> 00:30:58,080 +viral and viruses do not respond to antibiotics. + +413 +00:30:59,040 --> 00:31:04,820 +لكن هتقنع الناس إنه يروح على طبيب و يمشي على + +414 +00:31:04,820 --> 00:31:10,390 +الفلوس تعمال و مايكتبوش antibioticفللأسف احنا + +415 +00:31:10,390 --> 00:31:13,910 +بنشكل الضغط على الأطباء إنه يكتبونا ال antibiotic + +416 +00:31:13,910 --> 00:31:19,010 +بس النافذون الأطباء يكونوا وعين بدرجة كبيرة و + +417 +00:31:19,010 --> 00:31:22,810 +يرفضوا هذا الكلام لكن للأسف بيستجيبوا أيضا لل .. + +418 +00:31:22,810 --> 00:31:30,020 +use of antibiotics in animal feedقبل أقل من 6 أشهر + +419 +00:31:30,020 --> 00:31:33,300 +كان في ريبورتاج صحفي طلع على اللي .. اللي انا في + +420 +00:31:33,300 --> 00:31:36,520 +فلسطين هناك انه استخدام العقاقي و المناطق الحيوية + +421 +00:31:36,520 --> 00:31:39,280 +في الدواجن و المسا كبيرة احنا كنا في تلك الفترة + +422 +00:31:39,280 --> 00:31:43,500 +بنعمل دراسة على هذا الموضوع فكلمونا في وزارة + +423 +00:31:43,500 --> 00:31:46,260 +الزراعة وجالي أخواننا يعني قابلونا دورة ضيحة من + +424 +00:31:46,260 --> 00:31:49,060 +غير ما كان عندهم اي .. اي .. اي شي عشان كنتوا ال + +425 +00:31:49,060 --> 00:31:52,300 +.. ال .. ال .. المعلومات فاحنا عندنا معلومات علمية + +426 +00:31:52,300 --> 00:31:56,860 +دقيقة بفحصات وكميات وانواع من ال antibiotic فيهلكن + +427 +00:31:56,860 --> 00:31:59,500 +مش بالصورة اللى .. اللى .. اللى نشرت فى ال + +428 +00:31:59,500 --> 00:32:04,140 +laboratory لما أنا بدي ا .. ا .. اشتري دجاج او لحم + +429 +00:32:04,140 --> 00:32:07,460 +او اكبار دلوقتى دجاج انا بدي اكل لحم مابدي + +430 +00:32:07,460 --> 00:32:10,440 +antibiotic مابدي antibiotic لإن ال antibiotic قد + +431 +00:32:10,440 --> 00:32:14,600 +يسبب إلي أنا side effects منها حساسية ممكن يسببلي + +432 +00:32:14,600 --> 00:32:18,460 +abnormalities في ال normal flora صحيح؟ يبقى أنا + +433 +00:32:18,460 --> 00:32:22,560 +بحتاج دجاج بدون antibiotic او لحم بدون antibiotics + +434 +00:32:22,560 --> 00:32:24,380 +للأسف + +435 +00:32:27,920 --> 00:32:31,260 +المزارعين نظرتهم للدواجن مش زي نظرتنا للدواجن زي + +436 +00:32:31,260 --> 00:32:33,780 +ما زواجين تاكسي كده مش هوك زواجين تاكسين مش هواك + +437 +00:32:33,780 --> 00:32:37,360 +تمشي على الأرض بقولها .. بقولها انك رهيبة خالية + +438 +00:32:37,360 --> 00:32:41,600 +بقولها انا اتنين شكل فالدواجن + +439 +00:32:41,600 --> 00:32:45,160 +.. المربي الدواجن بيشوفوا الدواجن بطريقة مختلفة + +440 +00:32:45,160 --> 00:32:50,290 +جاتين يشتروا علفوزن جدّيش تحوّل لوزن دجاج، هما + +441 +00:32:50,290 --> 00:32:53,630 +بيسموه conversion rate، جدّيش سبعة ميل تحوّل العرف + +442 +00:32:53,630 --> 00:32:57,450 +الهقل، العرف أرخص و الدجاج أقل، فلو كان كيلو + +443 +00:32:57,450 --> 00:33:01,190 +بيجيبله كيلو بيكون perfect، طبعا هذا الكلام مستحيل + +444 +00:33:02,050 --> 00:33:06,370 +فلا يعني يراعوا + +445 +00:33:06,370 --> 00:33:12,090 +أنه في صحة بالأدمين على المحق في هذا الموضوع، فمش + +446 +00:33:12,090 --> 00:33:15,290 +بس بيستخدموا ال antibiotic في الهلاج، أصبحوا كمان + +447 +00:33:15,290 --> 00:33:18,490 +يستخدموه ك animal feed، مش animal feed + +448 +00:33:34,940 --> 00:33:40,740 +النقطة الأخيرة was failure to complete the + +449 +00:33:40,740 --> 00:33:41,800 +prescribed regimen + +450 +00:33:50,790 --> 00:33:54,550 +المشكلة .. المشكلة بقى إنه مش برتبة كمان، طبعا هو + +451 +00:33:54,550 --> 00:33:58,950 +غلط إن احنا نوقف، لازم إنه نكمل ال course، لآخر + +452 +00:33:58,950 --> 00:34:04,270 +يعني اكاسولة أو اخر حلقة whatsoever الشكل، الدلال، + +453 +00:34:04,270 --> 00:34:06,650 +لآخر جرعة، لآخر جرعة، لآخر جرعة، لآخر جرعة، لآخر + +454 +00:34:06,650 --> 00:34:07,070 +جرعة، لآخر جرعة، لآخر جرعة، لآخر جرعة، لآخر جرعة، + +455 +00:34:07,070 --> 00:34:11,170 +لآخر جرعة، لآخر جرعة، لأخر جرعة، لأخر جرعة، لأخر + +456 +00:34:11,170 --> 00:34:12,890 +جرعة، لأخر جرعة، لأخر جرعة، لأخر جرعة، لأخر جرعة، + +457 +00:34:12,890 --> 00:34:15,270 +لأخر جرعة، لأخر ج + +458 +00:34:19,760 --> 00:34:24,460 +اللي هو ال antibiotic من آخر، إذا في ممارسات + +459 +00:34:24,460 --> 00:34:29,760 +خاطئة، في ممارسات سيئة قد تساعد، قد تؤدي إلى زيادة + +460 +00:34:29,760 --> 00:34:36,220 +اللي هو ال .. لكن أنا أريد أن أختم بنقطة مهمة جدا، + +461 +00:34:36,220 --> 00:34:42,740 +وهي إن هناك دور حكومي مفقود تماما في الرقابة على + +462 +00:34:42,740 --> 00:34:44,320 +صرف الأدب، لا في العقاب + +463 +00:34:52,950 --> 00:34:57,210 +لو احنا بعتنا طفل طوله بيصارش ال counter تبع + +464 +00:34:57,210 --> 00:34:59,650 +الصيدلية ومعاه meat shake اللي هو ورقة مكتوب فيها + +465 +00:34:59,650 --> 00:35:03,610 +اسم الدواء بيبعتوه الدواء بدون prescription ال + +466 +00:35:03,610 --> 00:35:06,790 +antibiotic are prescription drugs بمعنى انه لا + +467 +00:35:06,790 --> 00:35:11,490 +تصرف الا بوصفة طبية وهذا كله للأسف مين مجدرجون فيه + +468 +00:35:11,490 --> 00:35:16,150 +فقط الحكومة طلقاء تشريع واتراقب هذا التشريع هذا + +469 +00:35:16,150 --> 00:35:22,590 +بحلم الأزمة هدا واحد رقم اتنينالانصياع لتعليمات + +470 +00:35:22,590 --> 00:35:28,710 +مندوبي الأدوية، يعني الطبيب بطل ياخد معلوماته من + +471 +00:35:28,710 --> 00:35:32,210 +الكتاب أو من الأبحاث أو من ال journal بياخد + +472 +00:35:32,210 --> 00:35:35,850 +معلوماته من مسوقي الأدوية، وبالتالي بقى رايش رجل + +473 +00:35:35,850 --> 00:35:43,760 +مبلغ هذا، ممنوعمندوب الأدوية يعطي الطبيب أدوية + +474 +00:35:43,760 --> 00:35:49,340 +حقيقية بيعطيه sample شكل منظر خارج مش الأهل بيعطيه + +475 +00:35:49,340 --> 00:35:53,100 +أهلا بيقولوا بكتابة بيقولوا اتفضل هدية هدية هدية + +476 +00:35:53,100 --> 00:35:56,660 +عشواء يعني هدية عشواء وبالتالي بصيب الفئة اللي هو + +477 +00:35:56,660 --> 00:36:01,920 +عشان كل مرة يمول عليه المندوب يعطيه الأدوية الهدية + +478 +00:36:01,920 --> 00:36:07,150 +بدى يصرف او يكتبره شتات منفهذا أيضا كمان من ضمن + +479 +00:36:07,150 --> 00:36:10,330 +الأنظمة اللي الأصل انها تتطور بحيث انها تمنع هذه + +480 +00:36:10,330 --> 00:36:14,670 +الظاهر المرة القادمة ان شاء الله هنشوف كيف + +481 +00:36:14,670 --> 00:36:19,890 +التفاعلات ال .. ال antibiotic بع بعضها واحد بيزورك + +482 +00:36:19,890 --> 00:36:23,470 +من جدام و واحد بيخرج من ورا مكانك سبب، مصبوط؟ + +483 +00:36:23,470 --> 00:36:27,690 +مكانك سبب، و ان شاء الله المرة القادمةبسم الله + +484 +00:36:27,690 --> 00:36:30,810 +الرحمن الرحيم السلام عليكم ورحمة الله وبركاته + +485 +00:36:30,810 --> 00:36:33,230 +اليوم سنبدأ محاضرة ال essential medical + +486 +00:36:33,230 --> 00:36:37,430 +microbiology بأسلوب غير عالمي معتاد اليوم أول + +487 +00:36:37,430 --> 00:36:41,990 +شخصية أهلا وسهلا بما نتشرف في لقائهم أهلا وسهلا + +488 +00:36:41,990 --> 00:36:46,590 +بزميلاتي وأصدقائي وأحبابي أهلا وسهلا بكم جميعا + +489 +00:36:46,590 --> 00:36:49,510 +اليوم + +490 +00:36:52,870 --> 00:36:57,550 +اليوم سنكشف لكم عن إحدى قنابل هذه الموصلة قضية + +491 +00:36:57,550 --> 00:37:01,510 +أثارت الجدل منذ زمن .. منذ زمن النبي العدنان .. + +492 +00:37:01,510 --> 00:37:05,590 +منذ زمن النبي العدنان عليه الصلاة والسلام حيث أنه + +493 +00:37:05,590 --> 00:37:09,130 +كان يشتكي من آلام تجعله يمكث ما بين اليوم واليومين + +494 +00:37:09,130 --> 00:37:13,290 +في بيته ولا يخرج وأنه عن ابن عباس رضي الله عنه قال + +495 +00:37:13,290 --> 00:37:17,730 +أنه كان يعصب عصبة على رأسه وأنه في حادث شدري قال + +496 +00:37:17,730 --> 00:37:22,310 +وفي حادث صحيحأنه قال في أثناء مرض موته ورأساه وكان + +497 +00:37:22,310 --> 00:37:26,130 +يعصب عصابة على رأسه وذكر أنه عليه الصلاة والسلام + +498 +00:37:26,130 --> 00:37:30,650 +ماشتكى إليه أحد من رأسه إلا وقال له احتجم وماشتكى + +499 +00:37:30,650 --> 00:37:34,810 +إليه أحد من ألمت في قدميه إلا وقال له تضب الحناء، + +500 +00:37:34,810 --> 00:37:38,990 +اليوم نزف لكم إحدى قنابل نفسيmicro organism صغيرة + +501 +00:37:38,990 --> 00:37:43,450 +استطعت أن تحل تلك القضية المثار عليها الجدال صعبت + +502 +00:37:43,450 --> 00:37:47,610 +على أهم ال antibiotic من حلها micro organism أراحت + +503 +00:37:47,610 --> 00:37:52,530 +و ناس طالما اشتكت من ألام شديدة لساعة طول لا لألام + +504 +00:37:52,530 --> 00:37:55,410 +صداع الشقيقة بعد اليوم no pain of migraine + +505 +00:37:55,410 --> 00:37:59,190 +headaches after today ماهو صداع الشقيقة صداع + +506 +00:37:59,190 --> 00:38:02,450 +الشقيقة هذه الكلمة مشتقة من اللغة اليونانية وهي + +507 +00:38:02,450 --> 00:38:07,110 +التي تعني ألام في الرأسو أيضا مشتقا من كلمة hemi + +508 +00:38:07,110 --> 00:38:11,910 +اللتي تعني نصف و carry on اللتي تعني جمجمة أما من + +509 +00:38:11,910 --> 00:38:15,930 +ناحية الطبية فهي tuat فهي تعني ألام شديدة تصبح في + +510 +00:38:15,930 --> 00:38:19,790 +نصف من الرأس الأيمن أو الأيسر مصاحبة بمجموعة من + +511 +00:38:19,790 --> 00:38:25,010 +الأعراضو من أهم الأعراض اللتي سنذكرها لكم سوية ما + +512 +00:38:25,010 --> 00:38:28,370 +يشعر به المريض في مرض ال migraine headaches هي + +513 +00:38:28,370 --> 00:38:32,270 +التقيق و حالة من ال vomiting و الغطيان كذلك يصبح + +514 +00:38:32,270 --> 00:38:36,110 +عنده ازدواجية في الرؤية كرؤية أن يرى الشيء ك zig + +515 +00:38:36,110 --> 00:38:40,130 +zag أن يراه على هيئة شيئين متقابلين أو شيئين فوق + +516 +00:38:40,130 --> 00:38:44,440 +بعضهم البعض كما أنه يشتكي منSensitive of the light + +517 +00:38:44,440 --> 00:38:48,820 +and fawn بما يسمى بالفوتوفوبيا and فونوفوبيا إضافة + +518 +00:38:48,820 --> 00:38:52,740 +إلى أعراض كثيرة قد يشتك منها سأطلب قعد السلام على + +519 +00:38:52,740 --> 00:38:56,200 +أسباب هذا المرض ومن أهم هذه الأسباب العوامل + +520 +00:38:56,200 --> 00:39:01,570 +الميراسيةإلى أهم .. أهم من ذلك، البيئة المحيطة، + +521 +00:39:01,570 --> 00:39:05,270 +حيث أن البيئة المحيطة تؤثر على نفسية الشخص بالسلب + +522 +00:39:05,270 --> 00:39:08,770 +أو الإيجاب، حيث تؤثر عليه بالسلب عندما يكون الشخص + +523 +00:39:08,770 --> 00:39:12,930 +في حالة اكتئاب أو أن أصبح لديه ظروف تجعل الشخص في + +524 +00:39:12,930 --> 00:39:17,350 +حالة نفسية سيئة، فيصبح من .. من أكتر عرضة لهذا + +525 +00:39:17,350 --> 00:39:22,570 +المرضبالإضافة إلى سبب مهم وجود خلل في جهاز الخدمة + +526 +00:39:22,570 --> 00:39:26,610 +و خاصة في ال answer يجعل الشخص معرض بشكل كبير إلى + +527 +00:39:26,610 --> 00:39:30,170 +الإصابة بهذا المرض بعض الدراسات كما تبين الصورة + +528 +00:39:30,170 --> 00:39:33,670 +بعض الدراسات القمت فيها أحد الأشخاص في .. أحد + +529 +00:39:33,670 --> 00:39:39,110 +الطلبة في جامعة عدن ال USAعلى مجموعة من الناس تبين + +530 +00:39:39,110 --> 00:39:44,690 +أن تقريبا 6% من الرجال مصابين بهذا المرض مقابل 18% + +531 +00:39:44,690 --> 00:39:48,110 +من النساء وهذا تدل على أن النساء أكثر عرضة + +532 +00:39:48,110 --> 00:39:52,490 +بالإصابة بهذا المرض لأن المرأة كائن حساس أكتر من + +533 +00:39:52,490 --> 00:39:57,880 +الرجالأما ال treatments للماجين of headaches أولا + +534 +00:39:57,880 --> 00:40:01,020 +نبدأ معاكم بال propranolol ال propranolol هو عبارة + +535 +00:40:01,020 --> 00:40:04,100 +عن دواء يعمل على ال tokens of the beta ال + +536 +00:40:04,100 --> 00:40:08,680 +baricitamol هذا دواء مضاد أو مسكّن عامال Evaliant + +537 +00:40:08,680 --> 00:40:12,380 +و هو مضاد الاكتئاب و ال Valerobic Acid و هو مضاد + +538 +00:40:12,380 --> 00:40:17,020 +التشنش و أخيرا الveramil و هو يعني دواء يعمل على + +539 +00:40:17,020 --> 00:40:20,240 +ال cutting or blocking of the calcium لم نستطيح + +540 +00:40:20,240 --> 00:40:23,820 +حصر كافة الأدوية المستخدمة لعلاج هذا المرض لكن + +541 +00:40:23,820 --> 00:40:27,900 +ذكرنا بما يكفي حتى تتوضح لذاكم الصورة لكن الإكتئاب + +542 +00:40:27,900 --> 00:40:32,600 +من هذه الأدوية تجعل الشخص معرض للإدمان مما يؤدي + +543 +00:40:32,600 --> 00:40:36,830 +ذلك إن جعل جهاز المناع دليل اتجاه هذه الأدويةو + +544 +00:40:36,830 --> 00:40:40,550 +هناك نوع من أنواع ال micro organism استطعت أن تحلل + +545 +00:40:40,550 --> 00:40:45,610 +لنا أحد أسباب ذلك المرض و هو مختص في ال gestric + +546 +00:40:45,610 --> 00:40:49,290 +tract و هو في الجهاز الهضمي بشكل أخص و هي + +547 +00:40:49,290 --> 00:40:52,490 +lactobacillus acetophilus lactobacillus + +548 +00:40:52,490 --> 00:40:55,930 +acetophilus كما نعلم و هي بكتيريا لبن الرائد تعد + +549 +00:40:55,930 --> 00:41:00,010 +gram positive bacteria fragmented sugar and lactic + +550 +00:41:00,010 --> 00:41:05,370 +acids تعيش في وسط بيئة يكون منخفضكما أن ال optimum + +551 +00:41:05,370 --> 00:41:08,730 +growth ده هي عبارة عن 37 درجة دزية، بالإضافة إلى + +552 +00:41:08,730 --> 00:41:11,710 +أن ال lactobacillus أسودوفلوس تعاد friendly + +553 +00:41:11,710 --> 00:41:16,750 +للبكتيريا ك normal flora تمام، normal flora تمام، + +554 +00:41:16,750 --> 00:41:20,110 +حيث أنها تعمل على إنتاج ال vitamin K للمعيدة + +555 +00:41:20,110 --> 00:41:24,490 +والكتازبالاضافة إلى ما قلته نرميل أن ال + +556 +00:41:24,490 --> 00:41:27,650 +lactobacillus acidophilus لها القدرة على تقلد + +557 +00:41:27,650 --> 00:41:32,550 +مستوى التوكسن أو النازل اللذي وجد فيه الدم + +558 +00:41:32,550 --> 00:41:38,890 +بالاضافة إلى أنها تقوم بتقليل من كمية ال pathogen + +559 +00:41:38,890 --> 00:41:43,510 +microorganism اللي توجد في جسم الإنسان علاقة على + +560 +00:41:43,510 --> 00:41:47,390 +ذلك لها القدرة إلى عمل fermentation للمنتجات + +561 +00:41:47,390 --> 00:41:51,330 +اللبانية أو خاصة اليوغراد بالاضافة لبعض من أنواع + +562 +00:41:51,330 --> 00:41:56,080 +الأشباطالليوبكتر بيلوري ماهي سبب ذكري لهذه + +563 +00:41:56,080 --> 00:41:58,940 +البكتيريا؟ هذه البكتيريا بالأخص يا نور هي نوع من + +564 +00:41:58,940 --> 00:42:02,080 +أنواع البكتيريا المسببة للهيلسرز الهيلسرز هي + +565 +00:42:02,080 --> 00:42:05,980 +القرحة قرحة المعيدة وكما ذكرنا مسبقا أن قرحة + +566 +00:42:05,980 --> 00:42:09,980 +المعيدة هي أحد الأسباب التي تسبب صداع نسبي أو ما + +567 +00:42:09,980 --> 00:42:13,220 +يسمى بال migraine وهو صداع الشقيقة الليوبكتر + +568 +00:42:13,220 --> 00:42:16,980 +بيلوري gram negative helic شهدت وكما نرى جانبا + +569 +00:42:16,980 --> 00:42:21,000 +أنها تمتلك للفلاجلة التي تساعدها على الحركةحيث + +570 +00:42:21,000 --> 00:42:25,800 +أنها تسبب المرض كذلك للإنسان يعني تقسم إلى لوعين + +571 +00:42:25,800 --> 00:42:28,480 +من المرض وهي ال chronic infections and acute + +572 +00:42:28,480 --> 00:42:31,760 +infections علاوة على ذلك فإنها لا تسبب فقط لا + +573 +00:42:31,760 --> 00:42:35,340 +تكتفي بتسبب المرض للإنسان إنها تتعالى أيضا و تسبب + +574 +00:42:35,340 --> 00:42:39,200 +المرض كذلك لل animals كما ذكرت أنها تسبب المرض ال + +575 +00:42:39,200 --> 00:42:42,160 +acute .. تسبب acute infection لإضافة ل chronic + +576 +00:42:42,160 --> 00:42:45,080 +infection ال acute infection من أهم الأمراض اللي + +577 +00:42:45,080 --> 00:42:48,600 +تتقوم بيها ان ال acute .. gastric acuteو قد تكون + +578 +00:42:48,600 --> 00:42:52,480 +مصاحبة لها بمجموعة من الأعراض منها abdominal open + +579 +00:42:52,480 --> 00:42:56,440 +بالإضافة لصداع في الرأس مع الغثيان قد يحدث في بعض + +580 +00:42:56,440 --> 00:43:00,740 +الأحيان غثيان تقيق كمان انه في بعض الأحيان تقوم + +581 +00:43:00,740 --> 00:43:06,020 +بال .. ال .. ال stool بكل لونه black color و أيضا + +582 +00:43:06,020 --> 00:43:10,200 +تكون مصاحبة بأعراض أخرى و من أهمها وجود حرقة في + +583 +00:43:10,200 --> 00:43:15,010 +الجهاز الهضمي و خاصة في منطقة الميقبالإعلاوة على + +584 +00:43:15,010 --> 00:43:18,670 +ذلك في مرض ال chronic infection حيث تسببه هذه + +585 +00:43:18,670 --> 00:43:23,490 +البكتيريا من هذه الأمراض المزمنة ال Peptic cancer, + +586 +00:43:23,610 --> 00:43:27,780 +Rest, Gastric cancer بالإضافة إلى ال lymphomaكما + +587 +00:43:27,780 --> 00:43:30,680 +أنها تعاد نوع من أنواع ال micro-aerophilic أي أنها + +588 +00:43:30,680 --> 00:43:34,060 +تحتاج إلى وجود الأكسجين ولكن بتركيز منخفضة جدا + +589 +00:43:34,060 --> 00:43:38,540 +إضافة إلى ذلك فإنها تمتلك ال hydrogenase تعمل على + +590 +00:43:38,540 --> 00:43:41,800 +إنتاج الطاقة، الطاقة تمتجها حتى تستطيع فيها الحركة + +591 +00:43:41,800 --> 00:43:45,940 +بواسطة الفلاجلة كما ذكرنا إضافة إلى ذلك فإنها تعمل + +592 +00:43:45,940 --> 00:43:49,360 +على أكسدة الهيدروجين حتى تعمل على إنتاج ال + +593 +00:43:49,360 --> 00:43:53,140 +intestinal bacteriaحتى نستطيع الكشف عنها نقوم + +594 +00:43:53,140 --> 00:43:57,120 +باستخدام مجموعة من الصبغات منها ال gram stain و + +595 +00:43:57,120 --> 00:44:00,760 +خبرة عنها بالإضافة ل ال gems stain وهي احدى ال + +596 +00:44:00,760 --> 00:44:04,900 +vital stain المستخدمة لإستفاقض الخلايا الحية ايضا + +597 +00:44:04,900 --> 00:44:08,020 +ال usain stain اللي استخدمت في معامل ال + +598 +00:44:08,020 --> 00:44:11,170 +microbiology و ال hematologyand the face contracts + +599 +00:44:11,170 --> 00:44:15,330 +microscopy وهو إحدى أنواع الميكروسكوب المستخدم + +600 +00:44:15,330 --> 00:44:17,950 +يتكشف عنه هو المعرف كذلك بmicroscope ال invert + +601 +00:44:17,950 --> 00:44:21,430 +microscope enter why into the stomach cavity and + +602 +00:44:21,430 --> 00:44:25,530 +causing ulcers يا ترى كيف تسبب ليه بكتر ما يسمى + +603 +00:44:25,530 --> 00:44:29,350 +بكرحة المعدة؟ إليكم التالي كما أنا علمت فإن موسط + +604 +00:44:29,350 --> 00:44:33,650 +المعدة يعاد acidic environment أي أنه يتمتع بدرجة + +605 +00:44:33,650 --> 00:44:37,610 +حموضية عاليةالسبب في ذلك حتى تعمل على حماية هذا + +606 +00:44:37,610 --> 00:44:40,590 +العضوم من وجود بكتيريا الخطيرة يعني غير ال normal + +607 +00:44:40,590 --> 00:44:43,990 +flora احنا متكلمة على البكتيريا الخطيرة حيث كذلك + +608 +00:44:43,990 --> 00:44:47,970 +يحتوي غشاء ال stomach على ما يسمى بال myosin ال + +609 +00:44:47,970 --> 00:44:51,070 +myosin هو نوع من أنواع البروتينات يعتبر gel أي + +610 +00:44:51,070 --> 00:44:54,430 +مادة هولانية لا تستطيع البكتيريا الحركة في داخله + +611 +00:44:54,430 --> 00:44:58,010 +يمسك في البكتيريا وكأنه يمنعها من الحركة ويتشبث + +612 +00:44:58,010 --> 00:45:01,870 +بيها بأن لا تدخل إلى المعيدة ولكن ال heliobacter + +613 +00:45:02,550 --> 00:45:06,810 +Biliary هو من أنواع البكتيريا الشرسة نوعا ما حيث + +614 +00:45:06,810 --> 00:45:10,130 +تعمل على تحويل الميوسينجل إلى مادة سائلة حتى + +615 +00:45:10,130 --> 00:45:16,230 +تستطيع بوسط الفلجلة الحركة ومغادرة المكان الأكثر + +616 +00:45:16,230 --> 00:45:20,590 +سمية بالنسبة لها وذهب إلى المكان الأكثر حساسية لها + +617 +00:45:20,590 --> 00:45:24,730 +إضافة إلى ذلك فإن ال helobacter biliary تقوم + +618 +00:45:24,730 --> 00:45:28,810 +باختراقجدار المعيدة معناه انها تختارت جدار المعيدة + +619 +00:45:28,810 --> 00:45:32,910 +انها تغادر من منطقة ال myosin تغادر من منطقة ال + +620 +00:45:32,910 --> 00:45:36,430 +myosin و ال gastric myosin gel حتى تصل لمنطقة ال + +621 +00:45:36,430 --> 00:45:41,100 +epithelial cellتبدأ تسبب فجوات وخروق في داخل + +622 +00:45:41,100 --> 00:45:45,080 +المائلة طب كما قلنا فإن هذا النوع من البكتيريا غير + +623 +00:45:45,080 --> 00:45:50,380 +محب لوسط ال environment acidic فماذا ستفعل بعد + +624 +00:45:50,380 --> 00:45:54,500 +ذلك؟ ستقوم بإنتاج ما يسمى بمادة الوريز، المادة + +625 +00:45:54,500 --> 00:45:58,320 +الوريز ستقسم إلى دايكسايد كاربون وتقسم إلى مادة + +626 +00:45:58,320 --> 00:46:02,200 +الأمونياالأمونيا عبارة عن basic طب وجود ال basic + +627 +00:46:02,200 --> 00:46:06,220 +مع وجود ال acidic حياتنا ال neutral فبالتالي فإن + +628 +00:46:06,220 --> 00:46:09,600 +هذا النوع من البكتيريا لها قدرة خارقة و ذكية على + +629 +00:46:09,600 --> 00:46:12,960 +استعمال الطرق البيو كيميائية لتحويل الوسط اللي + +630 +00:46:12,960 --> 00:46:16,560 +حولها و حماية نفسها نقل تصل إلى مطبقة ال betelial + +631 +00:46:16,560 --> 00:46:21,060 +cell حتى تحول الوسط ما حولها إلى وسط ال neutral pH + +632 +00:46:21,060 --> 00:46:25,660 +وبالتالي فإنها قد توجد إما على سطح ال epithelialأو + +633 +00:46:25,660 --> 00:46:29,440 +في داخل الابتليال وهذه البكتيريا ليست بقدرة ذكاء + +634 +00:46:29,440 --> 00:46:32,860 +بعض أنواع ال antibiotic منها ال homoverazole + +635 +00:46:32,860 --> 00:46:37,320 +بالإضافة ل ال amoxilane و الأشخاص اللتي تتوفر + +636 +00:46:37,320 --> 00:46:41,480 +لديهم حساسية ل البنسالين تقوم الدكاترة بإعطائهم ال + +637 +00:46:41,480 --> 00:46:44,480 +amoxilane مع ال metronidazole بالإضافة لل + +638 +00:46:44,480 --> 00:46:45,720 +levofloxane + +639 +00:46:47,680 --> 00:46:52,200 +والآن ما استطيع أن أجمل لكم ما تحدثنا عنه مرض ال + +640 +00:46:52,200 --> 00:46:55,760 +headaches، migraine ومرض ال ulcers هو مرض القرحة + +641 +00:46:55,760 --> 00:46:59,940 +كما قلنا مسبقا أن مرض ال ulcers وهو مرض القرحة أحد + +642 +00:46:59,940 --> 00:47:02,880 +الأسباب المؤدية لحدوث مرض ال migraine اللي هو + +643 +00:47:02,880 --> 00:47:06,900 +أمراض في الجهاز الهضمي تسبب مرض صداع النصفي أو ما + +644 +00:47:06,900 --> 00:47:10,120 +يسمى بصداع الشقيقة ولكن مع أن بكتيرالكتوبيسيلاس + +645 +00:47:10,120 --> 00:47:14,100 +استطعت أن تحل عقدة ومشكلة هذا المرض اللي هو ال + +646 +00:47:14,100 --> 00:47:19,340 +ulcers ومعالجة وعلاجة بتاعهبعلاج السبب نستطيع حل + +647 +00:47:19,340 --> 00:47:22,960 +اللي هو السبب الأكبر اللي هو ال migraine و كما + +648 +00:47:22,960 --> 00:47:26,700 +أثبتت الدراسات العلمية في جامعة ميلان في مدينة + +649 +00:47:26,700 --> 00:47:30,380 +إيطاليا حيث قامت بعمل إحصائية على مجموعة كبيرة من + +650 +00:47:30,380 --> 00:47:33,860 +الأشخاص المعانين من مرض ال migraine helix وجدوا أن + +651 +00:47:33,860 --> 00:47:37,440 +أغلبيتهم يشتكون من مرض ال cancers وهو مرض القرحة + +652 +00:47:37,440 --> 00:47:41,130 +في المعدةفقاموا بعمل دراسة على تلك الفئة، أعطوهم + +653 +00:47:41,130 --> 00:47:44,650 +ما يسمى بال lactobacillus acidophilus وهي بكتيرا + +654 +00:47:44,650 --> 00:47:49,430 +اللبن الرائب وبعد أن عدة شهور أو عدة أسابيع قاموا + +655 +00:47:49,430 --> 00:47:53,510 +بعمل حصات مرة أخرى وجدوا أن هؤلاء الأشخاص المصابين + +656 +00:47:53,510 --> 00:47:57,370 +بمرض ال migraine headaches قد شفوا منهم شفاء تعمل + +657 +00:47:57,560 --> 00:48:01,580 +والقسم الآخر قد خفت وحدت هذا الألم بنسبة كبيرة جدا + +658 +00:48:01,580 --> 00:48:04,800 +وكل ذلك يعتمد على الحدة وعلى ال environment، على + +659 +00:48:04,800 --> 00:48:07,600 +البيئة التي يعيشون بيها، ما بين الاكتئاب والتوتر + +660 +00:48:07,600 --> 00:48:13,970 +وما إلى ذلك هناك أشخاص مصابين بهذا المرضوما أكتر، + +661 +00:48:13,970 --> 00:48:20,530 +لكن من أصعب الشعور أن يكون شخص مقرب لدك ويكون مصاب + +662 +00:48:20,530 --> 00:48:25,370 +به، لأ ولم تكن بيداك أي حيلة، لكن اليوم استطعنا أن + +663 +00:48:25,370 --> 00:48:28,610 +نجد هذه الحيلة، اللهم اشفي جميع مرضانا، نهدي هذا + +664 +00:48:28,610 --> 00:48:31,970 +العمل بشكل أخص إلى الطالب عزيزي على قلوبنا جميع، + +665 +00:48:31,970 --> 00:48:36,300 +اللهم اشفي أحبتنا يا اللهو في الختم و أجمل ختم + +666 +00:48:36,300 --> 00:48:39,980 +بمسك السلام تصلوا علي الحبيب اللي عندنا نكون معاكم + +667 +00:48:39,980 --> 00:48:43,320 +نرميه العشاء و نور السفر مع تقديم فائقة شكرا جزيلا + +668 +00:48:43,320 --> 00:48:48,660 +لرفان الدكتور عبد الرؤم السلام عليكم طبعا تقديم + +669 +00:48:48,660 --> 00:48:54,170 +الصيغرية و لابتات وطيباتالزمان كان يقولوا قولي من + +670 +00:48:54,170 --> 00:48:58,970 +صاحبك أقولك من أنت اليوم بيقولوا قولي من ال normal + +671 +00:48:58,970 --> 00:49:02,250 +flora in your intestine أقولك من أنت حقيقة يعني + +672 +00:49:02,250 --> 00:49:08,370 +واضح أن ال evidence تتوالى باستمرار أنه على أهمية + +673 +00:49:08,370 --> 00:49:10,630 +ال normal flora و خاصة بال intestine التلاجة على + +674 +00:49:10,630 --> 00:49:17,570 +حفاظ على صحة جيدة يوم أن قمنا بتدخلكبشر وعطينا + +675 +00:49:17,570 --> 00:49:25,470 +مضادة حيوية وشكل مكتف وغيرنا من نوع الغذاء وطبيعته + +676 +00:49:25,470 --> 00:49:30,950 +تغيرت .. تغيرت الأمراض اللي تنتج لدى البشر ممكن أن + +677 +00:49:30,950 --> 00:49:33,610 +ال antibiotic تعمل على قتل ال normal flora كمان + +678 +00:49:33,610 --> 00:49:37,310 +يعني تاخد حبة من ال antibiotic ممكن غير أنها تقضي + +679 +00:49:37,310 --> 00:49:40,830 +على البكتيريبر وممكن تقضي على نفس ال normal flora + +680 +00:49:40,830 --> 00:49:44,350 +وبالتالي ممكن تسبب المرض للانتسنترا صح هذا .. هذا + +681 +00:49:44,350 --> 00:49:44,690 +ممكن + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..7220ce937782411310ecc7de83464526c4ac9708 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8_postprocess.srt @@ -0,0 +1,2360 @@ +1 +00:00:20,790 --> 00:00:24,910 +بسم الله الرحمن الرحيم الموضوع اليوم ما زلنا فيه + +2 +00:00:24,910 --> 00:00:30,610 +او اكمال لما يتعلق بالبعض genesis of بكتيريا ويمكن + +3 +00:00:30,610 --> 00:00:33,650 +المحاضرة السابقة ركزنا فيها اكتر شي على ال + +4 +00:00:33,650 --> 00:00:37,110 +exotoxins اليوم هيكون تركيزنا اكتر على ال + +5 +00:00:37,110 --> 00:00:40,050 +endotoxin و بعدين هنعمل مقارنة بين ال exotoxin و + +6 +00:00:40,050 --> 00:00:44,030 +ال endotoxin ونشوف ايش الفروقات الرئيسية ما بينهم + +7 +00:00:44,030 --> 00:00:45,030 +بشكل ملخص + +8 +00:00:47,450 --> 00:00:51,630 +الـ Lipopolysaccharide شفناها بأكتر بأكتر من شكل + +9 +00:00:51,630 --> 00:00:56,670 +لكن في المحصلة هو مكون من ثلاث أجزاء رئيسية اللي + +10 +00:00:56,670 --> 00:01:01,830 +هي Lipid-A وال core polysaccharide بالإضافة إلى + +11 +00:01:01,830 --> 00:01:09,250 +اللي هو ال O-side chain عشان يصير release للإندو + +12 +00:01:09,250 --> 00:01:13,490 +توكسين لازم يصير فيه cell wall lyses عشان يصير فيه + +13 +00:01:13,490 --> 00:01:16,110 +cell wall lyses غالبا لازم يكون فيه death + +14 +00:01:21,420 --> 00:01:26,020 +لكن في المحصلة ان ال release تبعه يكون ناتج او + +15 +00:01:26,020 --> 00:01:31,850 +ناجم عن اللي هو ال license تبع SLWالمُركّب قلنا + +16 +00:01:31,850 --> 00:01:35,130 +عنه formaldehyde and heat resistant وحاولنا نعمل + +17 +00:01:35,130 --> 00:01:39,790 +علاقة في المصطلح أو في الجملة هذه بين المعلومة + +18 +00:01:39,790 --> 00:01:44,830 +اللي ذكرناها عن التوكسويد في الإكسدوكسين التوكسويد + +19 +00:01:44,830 --> 00:01:49,870 +أنتجناه عبر معالدة بالformaldehyde أو بالحرارة + +20 +00:01:49,870 --> 00:01:53,790 +للاكسدوكسين لكن هنا فشلنا أن نحصل على توكسويد + +21 +00:01:53,790 --> 00:01:56,430 +لأنها resistant and formaldehyde resistant + +22 +00:02:01,160 --> 00:02:04,880 +المطمئن ايضا ان ممكن لو ضفنا adjuvant اللى هو + +23 +00:02:04,880 --> 00:02:09,140 +المادة ال carrier الحامل لو ركبنا على ال pre + +24 +00:02:09,140 --> 00:02:14,410 +-antigen ممكن نزيد من ال antigenicity تبعهفي + +25 +00:02:14,410 --> 00:02:16,890 +المحصلة النهائية اتفقنا ان ال life of + +26 +00:02:16,890 --> 00:02:20,430 +polysaccharide هو poor انتجين بالمقارنة مع اللي هو + +27 +00:02:20,430 --> 00:02:24,550 +ال exotoxin اللي طبيعي تكوينه أصلا protein وهذا + +28 +00:02:24,550 --> 00:02:28,270 +السبب الرئيسي في الفرق بين الاتنين ال protein is + +29 +00:02:28,270 --> 00:02:34,230 +more antigenic than اللي هو ال polysaccharide ال + +30 +00:02:34,230 --> 00:02:40,750 +endotoxin من أهم ال effects تبعته انه بيعملنا + +31 +00:02:40,750 --> 00:02:46,840 +feverو بيسموه pyrogen طلع الرقم اللي بدأ اتكلمكوا + +32 +00:02:46,840 --> 00:02:51,800 +عنه الان واحد microgram لكل kilogram كافين انه + +33 +00:02:51,800 --> 00:02:56,680 +يعملوا حرارة عند المرض واحد micro .. نتكلم عن + +34 +00:02:56,680 --> 00:02:59,520 +microgram .. micro .. نتكلمش عن milligram .. نتكلم + +35 +00:02:59,520 --> 00:03:06,780 +عن microgram طيب هدر الفبر .. الان لو طلعنا على ال + +36 +00:03:06,780 --> 00:03:10,440 +effect التاني اللي اسمه disseminated intravascular + +37 +00:03:10,440 --> 00:03:15,580 +coagulationأو باختصار DIC Disseminated + +38 +00:03:15,580 --> 00:03:19,600 +Intravascular Coagulation يعني جلطات منتشرة في + +39 +00:03:19,600 --> 00:03:26,990 +الجهاز الدوري للإنسان في كل مكانليش بيعمل هيك هو؟ + +40 +00:03:26,990 --> 00:03:29,570 +عنده Coagulase هو؟ فاكريني اللي Coagulase سابعة + +41 +00:03:29,570 --> 00:03:32,310 +السنة في الكوكاست بس هاد ال bacteria ماعندهاش + +42 +00:03:32,310 --> 00:03:36,130 +Coagulase عارفينيش عندها؟ عندها ال endotoxin عنده + +43 +00:03:36,130 --> 00:03:40,050 +مقدرة انه يعمل activation لل clotting cascade اللي + +44 +00:03:40,050 --> 00:03:45,850 +هي عملية التجلط في الدم المفروض انه بشكل طبيعي + +45 +00:03:45,850 --> 00:03:50,810 +تموان في الجروح لكن وجود ال endotoxin بيعمل + +46 +00:03:50,810 --> 00:03:52,870 +activation اللي هتوان في الدم + +47 +00:03:57,530 --> 00:04:01,630 +concentration of one milligram per kilogram of + +48 +00:04:01,630 --> 00:04:08,110 +endotoxin is lethal at one + +49 +00:04:08,110 --> 00:04:11,290 +milligram per kilogram طبعا هذا التركيز خدوا + +50 +00:04:11,290 --> 00:04:16,890 +بقالكوا بالمقارنة مع ال exotoxin تركيز عالي جدا ال + +51 +00:04:16,890 --> 00:04:21,570 +exotoxin قد يكون التركيز أقل من ذلك ويكون قاتل و + +52 +00:04:21,570 --> 00:04:22,650 +انا ذكرتلكم مثال + +53 +00:04:30,540 --> 00:04:40,010 +البوتلونيوم كاف لقتل عشرين مليون فرقمجرد تذوق، + +54 +00:04:40,010 --> 00:04:43,770 +وعارفين كلمة تذوق؟ قولنا مش أكل، مش تناول، تذوق + +55 +00:04:43,770 --> 00:04:48,010 +طعام بيحتوي على البوتراناتوكسين كافي لقتل الإنسان، + +56 +00:04:48,010 --> 00:04:52,510 +إذن احنا okay مواحد ملي جرام تركيز قليل، لكن ال X + +57 +00:04:52,510 --> 00:04:57,830 +-Toxin أقل منه كمان، كمان ال X-Toxin أقل طيب، من + +58 +00:04:57,830 --> 00:04:59,930 +ال effects تبعته انه بيعمل non-specific + +59 +00:04:59,930 --> 00:05:02,750 +inflammation عارفينش يعني non-specific + +60 +00:05:02,750 --> 00:05:06,210 +inflammation؟ يعني كل ال immune system بين الشغل + +61 +00:05:06,210 --> 00:05:10,310 +في حالةcytokine طبعا نجد عالمين عن cytokine + +62 +00:05:10,310 --> 00:05:15,190 +release اللي هو ال chemical messengers كمان بيعمل + +63 +00:05:15,190 --> 00:05:18,410 +حاسمة compliment activation و ياريت نفهم هذا + +64 +00:05:18,410 --> 00:05:21,170 +الجزئي للبعض اللي مادرسش مناعة مابيعرفش إيش يعني + +65 +00:05:21,170 --> 00:05:26,170 +compliment فبنوضح شو يعني compliment ال compliment + +66 +00:05:26,170 --> 00:05:30,830 +عبارة عن بروتينات موجودة في الجسم بشكل inactive + +67 +00:05:30,830 --> 00:05:34,970 +إيش يعني inactive؟ يعني مثل هذا القلم بالضبط طلع + +68 +00:05:34,970 --> 00:05:40,480 +عليها القلم هذاما حد بينكر انه قلب لكن لو حاولنا + +69 +00:05:40,480 --> 00:05:45,960 +نكتب فيه بالطريقة دى مش هيكتب لأنه inactive سبب + +70 +00:05:45,960 --> 00:05:49,340 +انه inactive انه فيه لغطى لو فكنا لغطى بصير ايش هو + +71 +00:05:49,340 --> 00:05:52,180 +اكتر طبعا من رحمة الله سبحانه وتعالى فينا من الجسم + +72 +00:05:52,180 --> 00:05:59,980 +انها inactive لأنه لا تنشط الا ب specific events + +73 +00:05:59,980 --> 00:06:04,240 +غالبا لقتل البكتيريا لقتل البكتيريا تخيل + +74 +00:06:04,240 --> 00:06:10,340 +اندروكسينش بيعمل بنشطهطب الاندوكسين بده يدي حاله؟ + +75 +00:06:10,340 --> 00:06:15,500 +لأ بنشطها في مواضع مش مناسبة للدسن فبالتالي بتقتل + +76 +00:06:15,500 --> 00:06:18,500 +خلايا مين؟ خلايا الدسن فبصير destruction لخلايانا + +77 +00:06:18,500 --> 00:06:21,360 +احنا بال complement activation طب شيه ال + +78 +00:06:21,360 --> 00:06:23,500 +complement احنا ماعرفناه عشان صحيح ال complement + +79 +00:06:23,500 --> 00:06:27,780 +هي عبارة عن group of proteins غالبا بيرمزولها ب C + +80 +00:06:27,780 --> 00:06:34,940 +فهي مرتبة من C1 ل C9 يعني C1, C2, C3 و هكذا انا مش + +81 +00:06:34,940 --> 00:06:39,670 +وصفتها زرجلالما بيصير ال activation غالبا بتنقسم + +82 +00:06:39,670 --> 00:06:44,330 +الجسمين fragment A و fragment V برضه اذا بيسموه + +83 +00:06:44,330 --> 00:06:49,670 +C5A C5V مثلا على ال complement خمسة A و كل واحدة + +84 +00:06:49,670 --> 00:06:52,990 +منهم بنات اللي هي function طبعا مش مجلة مش مجلة + +85 +00:06:52,990 --> 00:06:56,510 +لان نتكلم فيهم الآن هدرسوهم ان شاء الله في المناعة + +86 +00:06:56,510 --> 00:07:01,890 +بشكل دقيق و هتعرفوا كيف بيشتغلوا و كيف بيتنشطوا + +87 +00:07:01,890 --> 00:07:13,510 +أصلابيسل ميتوجين بيحفظ الخلايا على الإنقسام يعني + +88 +00:07:13,510 --> 00:07:20,870 +وجوده مدارمين للانقسام الكل + +89 +00:07:20,870 --> 00:07:26,710 +كلونال بيسل activators أول حاجة أرفها مش يعني + +90 +00:07:26,710 --> 00:07:33,980 +كلونال كلون معناها سلالة كلون معناها سلالةايه + +91 +00:07:33,980 --> 00:07:38,280 +سلالة بتكون؟ ناشعة من ايه؟ من one single cell، + +92 +00:07:38,280 --> 00:07:43,620 +سلالة، نوع معين، فلما نقول monoclonal بنقول سلالة + +93 +00:07:43,620 --> 00:07:49,810 +واحدة، لما نقول polyclonal عد سلالةالمفروض ان في + +94 +00:07:49,810 --> 00:07:54,230 +جهة الملعقة بتقولكوا كل حاجة اسمها specific فلما + +95 +00:07:54,230 --> 00:07:57,950 +احنا بننشط بننشط سلالة معينة من ال B-cells + +96 +00:07:57,950 --> 00:08:02,030 +لإنتاجها على سبيل المثال ال antibodies تخيلوا ان + +97 +00:08:02,030 --> 00:08:07,510 +الاندوكسين بيعمل activation لمجموعة كبيرة من ال B + +98 +00:08:07,510 --> 00:08:10,850 +-cells كل واحدة منها بتنتج سلالة لو افترضنا انهم + +99 +00:08:10,850 --> 00:08:15,830 +كلهم معينين لقيام بوظيفة واحدة هل يستطيعوا الالاف + +100 +00:08:15,830 --> 00:08:20,410 +اللي طلعت تجوم بهذه الوظيفة؟ لأ مستطيعوالأن + +101 +00:08:20,410 --> 00:08:24,790 +المخصصة لنفوت كونها one group فلما سينا دي + +102 +00:08:24,790 --> 00:08:29,110 +polyclonal B cell activator و B cell mitogen يعني + +103 +00:08:29,110 --> 00:08:32,330 +ثلاث مختلفة و complement activation و cytokine + +104 +00:08:32,330 --> 00:08:37,410 +release و non specific inflammationعشان هيك بنقول + +105 +00:08:37,410 --> 00:08:42,030 +احنا و بنذكركم لما دراسنا ال X-Toxin كنا نتكلم عن + +106 +00:08:42,030 --> 00:08:46,050 +one step كنا نقول ان it inhibits the release of + +107 +00:08:46,050 --> 00:08:50,410 +acetylcholine it inhibits the release of glycine + +108 +00:08:50,410 --> 00:08:54,420 +اللي بيمنع ال acetylcholineit can degrade + +109 +00:08:54,420 --> 00:08:58,580 +ribosomal RNA therefore it will prevent protein + +110 +00:08:58,580 --> 00:09:02,260 +synthesis it will inactivate elongation factor 2 + +111 +00:09:02,260 --> 00:09:05,220 +to prevent protein synthesis كل هذا بقى عن one + +112 +00:09:05,220 --> 00:09:09,900 +single step very specific step بس في ال endotoxin + +113 +00:09:10,580 --> 00:09:13,280 +الجاني بيتكلم على non specific inflammation، + +114 +00:09:13,280 --> 00:09:16,160 +بيتكلم عن fever، عن disseminated intravascular + +115 +00:09:16,160 --> 00:09:19,920 +coagulation، عن complement activation، عن B cell + +116 +00:09:19,920 --> 00:09:23,140 +mitogenes، عن polyclonal B cell activation، يفجر + +117 +00:09:23,140 --> 00:09:28,980 +في عندي عاصفة من الأحداث تنتج عن حضرة من ال + +118 +00:09:28,980 --> 00:09:36,980 +endotoxinهذا الشكل يعني بيعطينا التداخلات المتنوعة + +119 +00:09:36,980 --> 00:09:40,240 +للاندوكسين مش هراكز عليه كتير تقريبا كله ذكر في + +120 +00:09:40,240 --> 00:09:44,200 +اللوحات اللي هو السابقة لكن اللوحة اللي أمامنا هذه + +121 +00:09:44,200 --> 00:09:48,100 +مهمة كتير لإنها بتلخص الفروقات بين الاندوكسين + +122 +00:09:48,100 --> 00:09:51,620 +والإفستوكسين وانت تبقى نركز على اللوحة بشكل مستمر + +123 +00:09:51,620 --> 00:09:56,400 +حتى ننهي منها ان شاء لحظوا أول شيء ال chemical + +124 +00:09:56,400 --> 00:10:01,710 +natureال .. ال .. ال .. ال exoxin مكوّن من ال + +125 +00:10:01,710 --> 00:10:04,950 +protein بينما ال endoxin مكوّن من إيه؟ من ال + +126 +00:10:04,950 --> 00:10:07,390 +lipopolysaccharide، رحظة ال molecular weight تبع + +127 +00:10:07,390 --> 00:10:12,530 +التنين، أقل، أقل من الأقل؟ ال lipopolysaccharide + +128 +00:10:12,530 --> 00:10:16,950 +أقل من ال protein، وعلى فكرة أحد سمات التمييز في + +129 +00:10:16,950 --> 00:10:19,250 +ال antigenات، يعني لو ننصن في ال antigenات من + +130 +00:10:19,250 --> 00:10:23,770 +strong لبور، تمام؟الchemical structure له دور كبير + +131 +00:10:23,770 --> 00:10:28,050 +في التصنيف رقم اتنين ال molecular weight فاكر لما + +132 +00:10:28,050 --> 00:10:31,890 +قلت انه ال free molecule لو ركّبناها على carrier + +133 +00:10:31,890 --> 00:10:35,830 +بزيد من ال antigenicity تبقى ال size مهم، صح؟ ال + +134 +00:10:35,830 --> 00:10:41,250 +size مهم. relationship to cell علاقة التكسين + +135 +00:10:41,250 --> 00:10:44,450 +بالخلية ال endotoxin هو integral part + +136 +00:10:56,680 --> 00:11:01,680 +الخلية ينتج بالخلية من الداخل و بعدين يخرج الخلية + +137 +00:11:01,680 --> 00:11:09,440 +خارج الخلية while alive و الخلية لسه حية denatured + +138 +00:11:09,440 --> 00:11:12,940 +by boiling هل بيصيله denaturation طبعا لأنه + +139 +00:11:12,940 --> 00:11:17,300 +protein هناكاللي هو ال .. ال .. ال exoseme بصيرله + +140 +00:11:17,300 --> 00:11:20,300 +denaturation بينما الأولاني و ذكرناها في الأوحى + +141 +00:11:20,300 --> 00:11:23,840 +السابقة أنه لا بصيرله denaturation لا بال formalin + +142 +00:11:23,840 --> 00:11:29,780 +ولا بالهرارة و لأنه formalin and heat stay ال + +143 +00:11:29,780 --> 00:11:34,140 +antigenic yes و yes بس ممكن تضيفه كلمتين بعد ال + +144 +00:11:34,140 --> 00:11:39,220 +yes الأولانية poor و بعد ال yes التانية strongاحنا + +145 +00:11:39,220 --> 00:11:41,160 +بنميز بين التانية، لإن التانية عندهم antigenist + +146 +00:11:41,160 --> 00:11:45,000 +الصحية، لكن واحدة ضعيفة و واحدة قوية، مين الضعيفة؟ + +147 +00:11:45,000 --> 00:11:50,560 +ال .. ال endodoxin ال potency، الفعالية، مين أكثر + +148 +00:11:50,560 --> 00:11:57,380 +فعالية؟ ال exodoxin و ال endodoxin اتفقنا ك .. لا + +149 +00:11:57,380 --> 00:12:01,180 +.. لا .. انتوا الآن .. ه .. ه .. بدنا نشيل ال + +150 +00:12:01,180 --> 00:12:05,300 +confusion اللي حصل هذا ال confusion اللي حصل إنه + +151 +00:12:05,300 --> 00:12:09,570 +الفعالية غير عن ال mechanism of actionالـ + +152 +00:12:09,570 --> 00:12:13,210 +mechanism of action في ال-exotoxin كانت specific، + +153 +00:12:13,210 --> 00:12:16,870 +في ال-endoxin كانت multiple، صح؟ هنا بتتكلم عن + +154 +00:12:16,870 --> 00:12:20,490 +concentration capable of doing the job، يعني + +155 +00:12:20,490 --> 00:12:25,430 +التركيز اللي بيجوم بالمهمة، مين أقل تركيز، ال + +156 +00:12:25,430 --> 00:12:29,450 +-exotoxin تركيزه أقل، فبالتالي فعليته يكون أعلى، + +157 +00:12:29,450 --> 00:12:33,730 +هاي المفهوم اللي احنا بنقصه فيه في الـpotency، ال + +158 +00:12:33,730 --> 00:12:37,970 +specificity الآن low degreeأنا قلتلكوا أنا بعمل + +159 +00:12:37,970 --> 00:12:40,710 +non-specific inflammation و بيعمل fever و بيعمل + +160 +00:12:40,710 --> 00:12:44,110 +complement activation و cascade لل .. لل .. لل .. + +161 +00:12:44,110 --> 00:12:47,170 +لل clotting factor activation وبالتالي هو بالفعل + +162 +00:12:47,170 --> 00:12:50,130 +non-specific عارفين بيذكروننا بإياش؟ كويسة ال + +163 +00:12:50,130 --> 00:12:55,230 +analogy لما اتكلمنا عن ال .. ال antibiotics و + +164 +00:12:55,230 --> 00:12:59,290 +اتكلمنا عن ال disinfectantsلما اتكلمنا عن ال + +165 +00:12:59,290 --> 00:13:03,190 +disinfectant لجينا ان ال disinfectant بيقتل عن + +166 +00:13:03,190 --> 00:13:05,870 +طريق ال protein denaturation و بيقتل عن طريق ال + +167 +00:13:05,870 --> 00:13:09,210 +DNA نفس ال disinfectant الواحد بينما ال antibiotic + +168 +00:13:09,210 --> 00:13:15,510 +بكون له one specific step فبالتالي .. فبالتالي كان + +169 +00:13:15,510 --> 00:13:17,910 +سهل على البكتيري اللي بتذكر و بيبقى تعمل + +170 +00:13:17,910 --> 00:13:23,490 +resistance لمن؟ لل .. لل antibiotic لكان صعب تعمل + +171 +00:13:23,490 --> 00:13:26,210 +resistance لمن؟ ل .. اللي هو ال disinfectant + +172 +00:13:30,990 --> 00:13:34,630 +Enzymatic activity هل يمتلك التوكسين Enzymatic + +173 +00:13:34,630 --> 00:13:38,450 +activity بالنسبة للاندوتوكسين ايش الجواب؟ No بينما + +174 +00:13:38,450 --> 00:13:41,990 +ال X-Toxin و لو رجعتوا اللوحات من المحاضرة السابقة + +175 +00:13:41,990 --> 00:13:47,730 +ختمناها ب Enzyme نسمينا هم X-Toxin فال X-Toxin هم + +176 +00:13:47,730 --> 00:13:49,030 +بالفعل هم Enzymatic + +177 +00:13:51,870 --> 00:13:55,470 +ال pyrogenicity و ال pyrogenicity قدرة الميكروب + +178 +00:13:55,470 --> 00:13:58,730 +على رفع درجة حرارة البسم يعني حرارة شوف نجروا شوية + +179 +00:13:58,730 --> 00:14:05,250 +أنه ال endoxin بيعمل بينما ال exoxin مش كان .. + +180 +00:14:05,250 --> 00:14:08,930 +مابيعملش غالب لكن هنا .. هنا حاطين احنا جملة + +181 +00:14:08,930 --> 00:14:13,210 +occasionally لإنه في بعض ال exoxin قد ترفع درجة + +182 +00:14:13,210 --> 00:14:14,130 +اللي هو الحرارة + +183 +00:14:16,730 --> 00:14:20,030 +طيب هذا التركيب العظيم في ال gram negative بكتيريا + +184 +00:14:20,030 --> 00:14:24,670 +المسمى بال Lego polysaccharide أو ال endotoxin هل + +185 +00:14:24,670 --> 00:14:27,790 +يولد لهم مقابل counterpart في ال gram positive هو + +186 +00:14:27,790 --> 00:14:31,950 +خاص في ال gram negative صح؟ لأن هل لو counterpart + +187 +00:14:31,950 --> 00:14:36,470 +في إجابة بتقول أنا أقصده ولا نعم؟ بتقول yes اللي + +188 +00:14:36,470 --> 00:14:39,710 +بيبتدوا شرايكان اللي موجود في ال gram positive may + +189 +00:14:39,710 --> 00:14:43,150 +play a role similar to that of endotoxin of gram + +190 +00:14:43,150 --> 00:14:47,270 +negative bacteriaيعني .. يعني ال .. ال gram + +191 +00:14:47,270 --> 00:14:50,390 +positive الأصل أنه مالهاش ليه و polysaccharide طيب + +192 +00:14:50,390 --> 00:14:52,870 +ليش بتطلع حرارة؟ ليش بتعمل intra vascular + +193 +00:14:52,870 --> 00:14:55,990 +coagulation؟ ليش بتعمل .. لإن .. لإن ال + +194 +00:14:55,990 --> 00:14:59,590 +peptidoglycan تبعها بيشتغل بنفس الطريقة اللي + +195 +00:14:59,590 --> 00:15:02,950 +بيشتغل فيها مين أن هو ليه و polysaccharide تبع ال + +196 +00:15:02,950 --> 00:15:06,530 +gram negative؟ طيب enzymes + +197 +00:15:08,260 --> 00:15:11,080 +أظن نزجنا احنا من المحاضرة الماضية و هنقول + +198 +00:15:11,080 --> 00:15:16,320 +hyaluridase و collagenase و elastase انزيمات كلها + +199 +00:15:16,320 --> 00:15:20,460 +بتكسر الانسجار ليها tissue degrading enzymes لكن + +200 +00:15:20,460 --> 00:15:24,620 +اللي جديد في اللوحة دي شيء رائع جدا يجب أن ننتبه + +201 +00:15:24,620 --> 00:15:28,980 +له بشكل كتير انا افهم ان البعض منكم مادرسش مناعة + +202 +00:15:28,980 --> 00:15:32,300 +ولا عنده اي خلفيه عن ال immunoglobulins ال + +203 +00:15:32,300 --> 00:15:36,630 +antibodies الأجسام المضادةوبالتالي كانوا يتحملون + +204 +00:15:36,630 --> 00:15:39,250 +اللي .. اللي عندهم خلفيه في هذا الموضوع يتحملونها + +205 +00:15:39,250 --> 00:15:44,570 +البسم بينتج خمس أنواع من الأجسام المضادة بلاخصهم + +206 +00:15:44,570 --> 00:15:48,950 +في كلمة ماجد IG .. IG immunoglobulin ال + +207 +00:15:48,950 --> 00:15:52,410 +immunoglobulin هو وصف أخر لمين لل antibody يعني + +208 +00:15:52,410 --> 00:15:57,550 +الآن اللي قدامي على اللوحة هنا IGA immunoglobulin + +209 +00:15:57,550 --> 00:16:04,400 +Aفي من .. من بدل ال A كمان في أربع في IGG و IGM و + +210 +00:16:04,400 --> 00:16:10,500 +IGE و IGD و احنا قلنا ال A الخامس و سميناهم ماجد + +211 +00:16:10,500 --> 00:16:16,100 +حلوين ال antibodies انا لو بعرف انكم مش دارسينهم + +212 +00:16:16,100 --> 00:16:18,460 +كان عملتلكوا صورة برضه للموضوع لأنه مهم بالنسبة + +213 +00:16:18,460 --> 00:16:22,400 +لنا كتير مهم الموضوع اللي بهمني في اللوحة هذه ان + +214 +00:16:22,400 --> 00:16:27,580 +كنتوا عارفوا ان ال IGAهو ليس أهمهم على الإطلاق لكن + +215 +00:16:27,580 --> 00:16:34,840 +له ميزة مهمة جدا انه soluble مش انه soluble ذائب و + +216 +00:16:34,840 --> 00:16:37,740 +مدام ذائب فبنلاجيه في اللعاب بنلاجيه على mucous + +217 +00:16:37,740 --> 00:16:43,120 +membrane فهو الدفاع او خط الدفاع الأول ل specific + +218 +00:16:43,120 --> 00:16:49,800 +الأول اللي بيكون على ال membranes طيب الآن افترض + +219 +00:16:49,800 --> 00:16:54,260 +انه في عنديimmunoglobulin A تبع streptococcus + +220 +00:16:54,260 --> 00:16:57,420 +pneumonia، و الـ streptococcus pneumonia دخلت ال + +221 +00:16:57,420 --> 00:17:01,700 +lungs، أيش بيعمل فيها المفروض؟ بيمسك فيها و + +222 +00:17:01,700 --> 00:17:07,160 +بدمرها، فحضرتها بتجاية و جاهزة معاها سلاح يكسرها + +223 +00:17:07,160 --> 00:17:12,880 +السلاحيبقى ال IGA هو السلاح تبعنا احنا، سلاح + +224 +00:17:12,880 --> 00:17:17,720 +دفاعي، هي جايبه مع سلاح هجومي ضد الدفاعي، اللي هو + +225 +00:17:17,720 --> 00:17:23,080 +IGA Protease، انزين بيكسر ال immunoglobulin، تمام؟ + +226 +00:17:23,080 --> 00:17:26,940 +لن تستطيع إن هتعمل infection في ذاك المكان أو + +227 +00:17:26,940 --> 00:17:29,300 +attachment في ذاك المكان إلا بهذا السلاح، شوفوا + +228 +00:17:29,300 --> 00:17:33,000 +مجموعة كبيرة من الميكروبات الخطيرة جدا، اللي مرض + +229 +00:17:33,000 --> 00:17:37,890 +معاكوا أكتر مرة، زي Hemophilus influenzaستيكوكاس + +230 +00:17:37,890 --> 00:17:43,530 +نيمونيا نيسيريا جونريا و نيسيريا مينيديتس لن + +231 +00:17:43,530 --> 00:17:46,630 +يستطيعوا انهم يعملوا infection إلا بهذا السلاح + +232 +00:17:46,630 --> 00:17:51,110 +اللي هو ال IGA Proteins تمام؟ ثالثة معلومتان في + +233 +00:17:51,110 --> 00:17:56,670 +اللوحة هذه ان فيها انها soluble antibodies that + +234 +00:17:56,670 --> 00:17:59,310 +will protect mucous membranes و ال mucous membrane + +235 +00:17:59,310 --> 00:18:03,770 +ليش بدنا protection خاصة فيه؟بنفترض الممتاحة من + +236 +00:18:03,770 --> 00:18:08,270 +تبع العين أو الأنف أو الفم أو الورثرة أو ال vagina + +237 +00:18:08,270 --> 00:18:12,430 +من جوا مافيش فيهم skin و احنا بنتنا انت نتمتع + +238 +00:18:12,430 --> 00:18:17,710 +بالجلد برا كحماية فعشان نحمي هذه المناطق تمام بدنا + +239 +00:18:17,710 --> 00:18:23,570 +اللي هو السلاح اللي اسمه IGA لكن البكتيريا إجتوا + +240 +00:18:23,570 --> 00:18:29,670 +معاها counter weapon يعني سلاح مضاد لهذا السلاح + +241 +00:18:31,930 --> 00:18:37,290 +Anti-phagocytic factors عوامل مضادة لل .. لل .. لل + +242 +00:18:37,290 --> 00:18:40,970 +phagocytosis برضه كمان احنا الان اخدوا بالكم نتكلم + +243 +00:18:40,970 --> 00:18:47,510 +على احنا لدينا جهاز منع انتج IGA جيبناله سلاح كسره + +244 +00:18:47,510 --> 00:18:50,950 +انا عندي في جهاز المنع phagocytes و macrophages هي + +245 +00:18:50,950 --> 00:18:55,550 +الان نتكلم على كيفية مكافحة اللي هو ال phagocytes + +246 +00:18:55,550 --> 00:19:00,460 +و ال .. ال phagocytosis و ال macrophagesفي خدعة + +247 +00:19:00,460 --> 00:19:10,580 +قديمة كان زمان واحد ماعرف يالبس على كتفه مثلا جلد + +248 +00:19:10,580 --> 00:19:16,300 +خروف و يروح يعني يدخل بين الخرفان و يتخبى لسبب ما + +249 +00:19:16,300 --> 00:19:20,650 +يتخبى ف he is unrecognizedليش unrecognized؟ لأنه + +250 +00:19:20,650 --> 00:19:24,730 +استخدم material من المجموعة فتخيلوا إنه في بكتيريا + +251 +00:19:24,730 --> 00:19:28,530 +عندها المقدرة إنها تاخد من الانجر تبعتنا و تحط على + +252 +00:19:28,530 --> 00:19:32,870 +حالها أو من ال chemicals اللي تبعتنا و تحط الدهن + +253 +00:19:32,870 --> 00:19:36,870 +حالها على الأساطر تبعها و بتصير لزق منها فهيكد + +254 +00:19:36,870 --> 00:19:42,790 +تبقى لمن؟ بالظبط زي أنا أعطيكم مثال بسيط لما أخدنا + +255 +00:19:42,790 --> 00:19:46,110 +الجلطات من الكواجوليز في الستفيل الكوكاس أول + +256 +00:19:46,110 --> 00:19:50,710 +مناسبة هحطكم بقى فيديو على الفيسبوكاسمه .. اسمه + +257 +00:19:50,710 --> 00:19:55,770 +الفيديو Virulence Factor لـ Staphylococcus aureus + +258 +00:19:55,770 --> 00:19:58,570 +و أنا بعتقد أنه لازم تشوفوه كلكوا لإنه بيوضّيكوا + +259 +00:19:58,570 --> 00:20:02,410 +ال .. ال Virulence Factor كميكايزم of action بي .. + +260 +00:20:02,410 --> 00:20:04,850 +بي .. بي .. في كل خطوة من خطوات ال infection من + +261 +00:20:04,850 --> 00:20:09,650 +دخول البكتيريا إلى ال dissemination تبعت على ال .. + +262 +00:20:09,650 --> 00:20:12,090 +ال .. الموقع تبعتنا، المجموعة ده اللي هي Essential + +263 +00:20:12,090 --> 00:20:17,170 +Medical Microbiology F أمام؟ اللي هي الفيميلطيب + +264 +00:20:17,170 --> 00:20:20,890 +النوع التاني few bacteria produces soluble factors + +265 +00:20:20,890 --> 00:20:24,830 +or toxins that inhibit chemotaxis by leukocytes + +266 +00:20:24,830 --> 00:20:31,390 +and thus evade phagocytosis الآن ال cytokines + +267 +00:20:31,390 --> 00:20:37,750 +وظيفتها الرئيسية chemical messengers خلية أصيبت + +268 +00:20:37,750 --> 00:20:40,890 +بستمر release ل cytokines ال cytokines بتقول ال + +269 +00:20:40,890 --> 00:20:43,790 +inflammatory cells ومن ضمنها ال phagocytes تعالوا + +270 +00:20:43,790 --> 00:20:47,630 +إلى هذا المكان هناك بكتيرياموجودة في المكان + +271 +00:20:47,630 --> 00:20:54,470 +البكتيريا ما عندهاش سلاح لمكافحة الفاجوسايت او + +272 +00:20:54,470 --> 00:21:00,090 +للبقاء حي جوا الفاجوسايت ايش بتعمل؟ بتنتج chemical + +273 +00:21:00,090 --> 00:21:05,630 +هذا ال chemical بيفهم الفاجوسايت ان هذا false + +274 +00:21:05,630 --> 00:21:11,790 +alarm يعني انضار خاطر انضار خاطر صعب حالة بترجع + +275 +00:21:11,790 --> 00:21:17,610 +الفاجوسايتلغة Chemicals بنبعت لبعض نخترخ جوّالات + +276 +00:21:17,610 --> 00:21:20,630 +بعض و بنبعت لبعض مزيجيز مضادة واحد بيقولي تعالي و + +277 +00:21:20,630 --> 00:21:26,210 +انا بقوله ياه ماتيجيش فالبكتيريا بتنتج Chemicals + +278 +00:21:26,210 --> 00:21:29,630 +to prevent the chemotactic activity of اللي هو + +279 +00:21:29,630 --> 00:21:35,750 +الخلايا تبعها لكن تعالوا نطلع على اللوحة هذه اللي + +280 +00:21:35,750 --> 00:21:40,830 +فيها معلومات ضخمة و كثيرة عن عوامل محددة تستخدم ك + +281 +00:21:40,830 --> 00:21:42,190 +antifagocytic + +282 +00:21:44,000 --> 00:21:47,180 +درست واحدة منها مهمة جدا اللي هي ال capsule، اعتقد + +283 +00:21:47,180 --> 00:21:51,500 +انه كل كوب عرفت ان الكابسول أحد مهمها كان لها + +284 +00:21:51,500 --> 00:21:55,840 +وظيفته لأي سيطرة، مظبوط، أشوش هما antifagocytosis + +285 +00:21:55,840 --> 00:22:00,640 +و التانية attachment، و يوم ما جولنا عكس بعض + +286 +00:22:00,640 --> 00:22:05,720 +التنتين صح؟ طيب ال polysaccharide capsule of + +287 +00:22:05,720 --> 00:22:10,580 +streptococcus pneumoniaHemophilus تقول أسمها بدأت + +288 +00:22:10,580 --> 00:22:13,380 +تتكرر معانا و صارت familiar الأسمها Hemophilus + +289 +00:22:13,380 --> 00:22:19,080 +influenza Triponema palidum Bacillus anthracis اه + +290 +00:22:19,080 --> 00:22:22,560 +في غلطة كبيرة هنصحيها حطوا خط تحت Bacillus + +291 +00:22:22,560 --> 00:22:27,300 +anthracis و اكتبوا عليها exception مش + +292 +00:22:27,300 --> 00:22:31,160 +polysaccharide هي مش polysaccharide هي polypeptide + +293 +00:22:31,160 --> 00:22:32,400 +polypeptide + +294 +00:22:36,750 --> 00:22:39,170 +and Klebsiella pneumonia بعرف في المعمل اللي + +295 +00:22:39,170 --> 00:22:44,510 +بتعملته capsule stain in the ink شفته طبعا أكيد + +296 +00:22:44,510 --> 00:22:46,450 +البكتيريا اللي جابولكوا إياها جابولكوا اللي هو ال + +297 +00:22:46,450 --> 00:22:49,810 +Klebsiella جابولكوا ال Klebsiella وشفته مصطلعات ال + +298 +00:22:49,810 --> 00:22:51,210 +Klebsiella ال large + +299 +00:22:53,540 --> 00:22:57,560 +Large و Mucoid عارفين إيش بيسموا ال .. ال .. ال .. + +300 +00:22:57,560 --> 00:23:01,980 +ال polysaccharide المكون للكبسول بعض الناس بيسموه + +301 +00:23:01,980 --> 00:23:05,760 +Mycopolysaccharide ليش؟ لأنه ال .. ال large + +302 +00:23:05,760 --> 00:23:10,200 +quantity منه ميوكايد و كلمة ميوكايد لزج صح؟ و + +303 +00:23:10,200 --> 00:23:15,480 +النزوج عامل بيساعد بإنه على عدم قدرة التساق الفاجو + +304 +00:23:15,480 --> 00:23:19,380 +ساعد بيه البكر المهم إن الجملة هذه اللي مارين + +305 +00:23:19,380 --> 00:23:23,260 +عليها المرسلية محتوية على معلومات خطيرة كثيرةمن + +306 +00:23:23,260 --> 00:23:27,520 +ضمنها أسماء لمجموعة من الميكروبات كلها بتحمل على + +307 +00:23:27,520 --> 00:23:30,560 +ظهرها اللي هي E-capsule فبنكون عارفين برضه انها + +308 +00:23:30,560 --> 00:23:36,800 +Decapsulated microorganism في تراكيب خاصة protein + +309 +00:23:36,800 --> 00:23:40,860 +M أو M-protein و الفمبريا في ال Streptococcus + +310 +00:23:40,860 --> 00:23:43,560 +biogenes اللي بتنتمي إلى مجموعة بيسميها group A + +311 +00:23:43,560 --> 00:23:46,200 +streptococci هدولة بيساعدوها على الغروب برضه من + +312 +00:23:46,200 --> 00:23:51,740 +الفاجوه سايتوسPseudomonas aeruginosa رقم تلاتة + +313 +00:23:51,740 --> 00:23:56,380 +Pseudomonas aeruginosa بتعمل biofilm اعتقد ان انا + +314 +00:23:56,380 --> 00:24:00,100 +مرة يعني .. بس انا حابب ان اؤكد على ان ال biofilm + +315 +00:24:00,100 --> 00:24:06,400 +هو عبارة عن ال aggregation لال micro organism و + +316 +00:24:06,400 --> 00:24:10,680 +اؤكد على حقيقة ان تصرف البكتيريا ك biofilm بيختلف + +317 +00:24:10,680 --> 00:24:18,350 +عن تصرفها ك single microorganism اش يعني بيختلف؟لو + +318 +00:24:18,350 --> 00:24:23,390 +أنا عملت اختبار شفوي لحضاراتكم وكل طالب دخل علي + +319 +00:24:23,390 --> 00:24:28,590 +المكتب وسألتها سؤال أكيد فرصتها فيه لإن الجاوب صح + +320 +00:24:28,590 --> 00:24:34,270 +بتكون أقل من فرصة لو هي اتشاورت مع زميلة إلها ولو + +321 +00:24:34,270 --> 00:24:38,070 +اتشاورت مع كل الفصل بتزيد فرص انك تحصلوا إجابة إيه + +322 +00:24:38,070 --> 00:24:40,730 +طبعا أنا ماتشجعش الأسلوب هذا في الامتحانات لو + +323 +00:24:40,730 --> 00:24:44,760 +اتشاور مع بعضلكن في الآخر هذا الواقع اللي بيصير لو + +324 +00:24:44,760 --> 00:24:48,940 +احنا بدنا نطلع برأي معين في اجتماع اكيد الرأي + +325 +00:24:48,940 --> 00:24:53,220 +بيكون افضل اما يكون اكترالبكتيريا بلات لما بتكون + +326 +00:24:53,220 --> 00:24:56,760 +مكونة biofilm تتعاملش ك single cells، بتتعامل ك + +327 +00:24:56,760 --> 00:25:00,660 +multicellular organism، يعني ك .. ك .. ك .. ك .. + +328 +00:25:00,660 --> 00:25:05,860 +كائن ذو خلايا متعددة، يعني زي ده، إلا دماغ و إلا + +329 +00:25:05,860 --> 00:25:10,040 +عينين و إلا إيدين، فبتتصرف بهذا ال .. بهذا السلوك، + +330 +00:25:10,040 --> 00:25:13,300 +وهذا التصرف بهذا السلوك بيؤدي إلى أن أدائها بيكون + +331 +00:25:13,300 --> 00:25:17,460 +أحسنإيش نقلدها أحسن؟ ال survival تبعها في ال + +332 +00:25:17,460 --> 00:25:21,800 +environment بيكون أفضل، مقاومتها للعوامل الجارحة، + +333 +00:25:21,800 --> 00:25:24,180 +يعني ال injurious agents، ال chemicals أو الحرارة، + +334 +00:25:24,180 --> 00:25:28,560 +بتكون أفضل من مما لو كانت single cell فحتى + +335 +00:25:28,560 --> 00:25:32,120 +مقاومتها لل phagocytosis لإنها تنتج المواد اللي + +336 +00:25:32,120 --> 00:25:36,100 +بتستخدمها لإنها تربط حالة بعض، برضه عبارة عن + +337 +00:25:36,100 --> 00:25:39,830 +polysaccharideوال polysaccharide برضه بيكون ميكويد + +338 +00:25:39,830 --> 00:25:44,510 +فبسهل أو بصعب عملية الفاجو أفوان سايتوس وبسهل + +339 +00:25:44,510 --> 00:25:49,510 +عملية الهروب بتتذكروا الليبو بوليساكرايد كان مكوّن + +340 +00:25:49,510 --> 00:25:52,650 +من ال lipid A core polysaccharide O sided chain ال + +341 +00:25:52,650 --> 00:25:55,730 +O sided chain على سبيل المثال في الليبو + +342 +00:25:55,730 --> 00:25:58,990 +بوليساكرايد لبعض ال gram negative بكتيريا برضه + +343 +00:25:58,990 --> 00:26:02,670 +عامل مساعد على الهروب من الفاجو سايتوس زي + +344 +00:26:02,670 --> 00:26:06,740 +الاشريشيا coli ما هو ذكره عنهافي K أنتجين و K + +345 +00:26:06,740 --> 00:26:12,060 +أنتجين هو حقيقة كابسولر أنتجين ويله مقابل في + +346 +00:26:12,060 --> 00:26:16,580 +السلمونين اللتيفي مسمى مشهور اسمه V-I أنتجين يبقى + +347 +00:26:16,580 --> 00:26:21,740 +ال K و ال V-I بيشبهوا بعض و كلاهما تعبير عن كابسول + +348 +00:26:21,740 --> 00:26:25,300 +بس صغيرة micro capsule + +349 +00:26:26,890 --> 00:26:30,750 +برضه بيساعد على الهروب طبعا شوفوا التفاصيل و + +350 +00:26:30,750 --> 00:26:35,770 +الكلمات الفطفاضة و اللي بتمر معنا مرور سريع بكرا + +351 +00:26:35,770 --> 00:26:39,670 +هتتذكرها هبتعطيكوا مثال صغير لان عشان في ال + +352 +00:26:39,670 --> 00:26:44,380 +diagnostic هتسمعوا الجملة دي اللي حاكلكواالإشريشيا + +353 +00:26:44,380 --> 00:26:48,300 +كولاي normal flora of the intestine لكن number one + +354 +00:26:48,300 --> 00:26:51,340 +cause of urinary tract infection البعض منها ممكن + +355 +00:26:51,340 --> 00:26:54,380 +يعمل neonatal meningitis إيش هي neonatal + +356 +00:26:54,380 --> 00:26:59,860 +meningitis؟ التهاب، سحاية عند الأطفال، حدثه + +357 +00:26:59,860 --> 00:27:06,740 +الولادة ويردوا إن كل العزلات كل العزلات اللي + +358 +00:27:07,440 --> 00:27:10,560 +بيعزلوها من الأطفال اللي عندهم neonatal meningitis + +359 +00:27:10,560 --> 00:27:14,220 +وردوا أن ال equalize السلالة يعني بتنتج K capsule + +360 +00:27:14,220 --> 00:27:17,760 +يبقى ال K capsule هو اللي أهلها تعمل neonatal + +361 +00:27:17,760 --> 00:27:21,920 +meningitis وسط صورتها one single factor ووجد في + +362 +00:27:21,920 --> 00:27:25,440 +البكتيريا أعطاها قدرة أكتر منها من زملاتها الأخرى + +363 +00:27:26,640 --> 00:27:31,180 +رقم ستة هو الرائع والجميل الحلو كتير اللي فيه فكرة + +364 +00:27:31,180 --> 00:27:36,680 +وفيه يعني إعجاز حقيقة نطلع على ال antibody اللي + +365 +00:27:36,680 --> 00:27:40,860 +احنا شفناه جاب شوية ال antibody بنفترض انه بهذا + +366 +00:27:40,860 --> 00:27:44,740 +الشكل اللي antigen binding side يعني ال antibody + +367 +00:27:44,740 --> 00:27:48,520 +هذا الطرف تبعه هي ال antigen تبعه بيجي برتبط بهذا + +368 +00:27:48,520 --> 00:27:55,380 +الشكلالجهة الخلفية من الـ Antibody هذي بتشكل + +369 +00:27:55,380 --> 00:28:00,260 +ريسبتور للفاجوسايتز والماكروفايريز فالان البكتيريا + +370 +00:28:00,260 --> 00:28:04,060 +مرتبطة بهذا الشكل بالـ Antigen + +371 +00:28:12,920 --> 00:28:16,360 +مين بيجي يرتبط المفروض ال macro phage ترتبط بهذا + +372 +00:28:16,360 --> 00:28:19,020 +الجزء يعني .. يعني .. يعني كل ال macro phage و ال + +373 +00:28:19,020 --> 00:28:21,720 +phagocytes اللي هي receptors في هذا الجزء بيسموه + +374 +00:28:21,720 --> 00:28:26,040 +Fc portion فلما تلجي ترتبط فيه حتى لو البكتيريا + +375 +00:28:26,040 --> 00:28:30,820 +إليها capsule حتى لو بتهرب من أي حاجة بنام صار جسر + +376 +00:28:30,820 --> 00:28:34,180 +بينها و بين جسر اللي هو ال antibody بينها و بينها + +377 +00:28:34,180 --> 00:28:38,000 +بتلتقموا على طول فإيش ال .. إيش البكتيريا بتسوي + +378 +00:28:38,000 --> 00:28:40,680 +هذا اللي اسمها Staphylococcus aureus بتنتج protein + +379 +00:28:40,680 --> 00:28:45,340 +اسمه protein إيه؟هذا كمناك بروتيوم مش هتلاجمه، هذا + +380 +00:28:45,340 --> 00:28:47,500 +ممسك الانتبار يبقى برج طب يجلبه زيك + +381 +00:28:50,380 --> 00:28:53,640 +ويربط بنفس ال .. ال .. ال .. ال receptor تبع ال + +382 +00:28:53,640 --> 00:28:57,140 +macro phage تمام؟ و الفاج و ال size ايش بيصير فيه + +383 +00:28:57,140 --> 00:29:01,880 +فوجة معلجة هنا؟ فاضي .. فاضي .. فاضي .. فاضي .. + +384 +00:29:01,880 --> 00:29:01,960 +فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. + +385 +00:29:01,960 --> 00:29:02,140 +فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. + +386 +00:29:02,140 --> 00:29:02,240 +فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. + +387 +00:29:02,240 --> 00:29:05,120 +فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. + +388 +00:29:05,120 --> 00:29:17,860 +فاضي .. فاضي .. فاضي .. فاضي + +389 +00:29:17,860 --> 00:29:22,770 +..الارتباط ال normal لل antigen أو لل antibody هو + +390 +00:29:22,770 --> 00:29:26,270 +عبر ال antigen binding site بهذا الشكل و على الطرف + +391 +00:29:26,270 --> 00:29:30,930 +التاني بيشكل receptor لمين؟ للماكروفاج ال protein + +392 +00:29:30,930 --> 00:29:37,210 +A بياخد ال FC portion هذا و بيرتبط فيه و بيخلي هذا + +393 +00:29:37,210 --> 00:29:41,630 +periphery وبالتالي لن يرتبط الماكروفاج أو الفاجو + +394 +00:29:41,630 --> 00:29:46,710 +صحيحإذا هذه حركة ذكية من ال Staphylococcus aureus + +395 +00:29:46,710 --> 00:29:51,410 +بإنها تعمل neutralization لل antibody اللي ممكن + +396 +00:29:51,410 --> 00:29:56,870 +يسهل في عمل اللي هو الفاجو سايتاسيس أعتقد أنه + +397 +00:29:56,870 --> 00:30:00,730 +أجهزت نفسي و أنا بشرح و أحاول بالجلم و هيفين صورة + +398 +00:30:00,730 --> 00:30:03,910 +على الصبورة فكويس أني أطلع عليها هاي + +399 +00:30:03,910 --> 00:30:09,210 +Staphylococcus مثلا Epidulis و هذه Aureus التانية + +400 +00:30:09,930 --> 00:30:14,330 +لاحظوا ال E-Dermatitis طبعا ماعندهاش protein A + +401 +00:30:14,330 --> 00:30:18,870 +فبالتالي هي اللي هو ال .. ال .. ال .. ال antibody + +402 +00:30:18,870 --> 00:30:22,830 +مرتبط بال antigen مرتبط بال antigen هنا وهي ال + +403 +00:30:22,830 --> 00:30:26,390 +phagocytes المفروض مرتبط بمين لها؟ بال Fc .. في ال + +404 +00:30:26,390 --> 00:30:29,850 +antibody في ال antibody فبتكوّن un dessert منهم + +405 +00:30:29,850 --> 00:30:32,990 +طلعوا لنا في الصورة هذه بتكوّن هل ال macro phage + +406 +00:30:32,990 --> 00:30:36,570 +هي ال dessert؟وهي ال antibody طبعا ال antibody هذا + +407 +00:30:36,570 --> 00:30:40,590 +ثنائي الشعبة اللي مابيعرفوش في خماس الشعبة كمان بس + +408 +00:30:40,590 --> 00:30:44,310 +هذا ثنائي الشعبة او يعني two antigen binding sites + +409 +00:30:44,310 --> 00:30:49,350 +ليش صار فندي هنا ال macrophage مسكت اتنين بكتيريا + +410 +00:30:49,350 --> 00:30:53,530 +عبر one single antibody ع طول الأقعد لو كان لهم + +411 +00:30:53,530 --> 00:30:55,950 +capsule بقى و مش capsule اللي هم ايش ما كان لهم + +412 +00:30:55,950 --> 00:30:59,790 +كان ساقونهم قرروا تنفيب انهم ليش لقاعد حادث الخطوة + +413 +00:30:59,790 --> 00:31:01,850 +الأولى اللي هي ال attachment ال attachment جدا مهم + +414 +00:31:01,850 --> 00:31:06,020 +جدا في هذا الموضوعهذه لما بكتيريا بتنتج من روتين A + +415 +00:31:06,020 --> 00:31:08,880 +يجي لبكتيريا التانية البكتيريا التانية استفد ل + +416 +00:31:08,880 --> 00:31:12,340 +Cocas Aureus ومن النوع اللي بنتج من روتين A إيش + +417 +00:31:12,340 --> 00:31:16,580 +عمل روتين A؟ روتين A أخد ال antibody و ارتبط فيه + +418 +00:31:16,580 --> 00:31:20,040 +هل له ريسيبترو الوكروف الفارغوسايتز؟ مالوش + +419 +00:31:20,040 --> 00:31:26,600 +ريسيبترو فبالتالي عمل تحييد لفارغوسايتز وعمل تحييد + +420 +00:31:26,600 --> 00:31:30,940 +ل ال macro phage من خلال ارتباطه بال up C portion + +421 +00:31:31,160 --> 00:31:33,480 +اللي هو protein A و ال protein A موجود على ال + +422 +00:31:33,480 --> 00:31:37,160 +surface تبع ال Staphylococcus aureus لذلك بنلاقي + +423 +00:31:37,160 --> 00:31:42,360 +احنا ال pathogenicity تبع ال Staphylococcus aureus + +424 +00:31:42,360 --> 00:31:45,020 +أعلى بكتير من ال pathogenicity تبع ال + +425 +00:31:45,020 --> 00:31:49,200 +Staphylococcus epigenesis و زي ما حتى كنا بقولها + +426 +00:31:49,200 --> 00:31:52,780 +non pathogenic بينما دي pathogenic + +427 +00:31:56,340 --> 00:32:00,080 +ال M-protein اللى شايفينه قدامنا هنا بيشتغل برضه + +428 +00:32:00,080 --> 00:32:05,080 +بطريقة انه يمنع اللى جونه عنه بيمنع ال .. ال .. ال + +429 +00:32:05,080 --> 00:32:11,820 +phagocytosis و + +430 +00:32:11,820 --> 00:32:15,620 +بيعمل عملي تنشيط لمين؟ لل compliment و أنا هذكركم + +431 +00:32:15,620 --> 00:32:19,840 +بموضوع ال compliment compliment مرة أخرى بنات هي + +432 +00:32:19,840 --> 00:32:25,900 +..جروبو بروتين لكن هي non-active، ال activation + +433 +00:32:25,900 --> 00:32:30,620 +تبعها بيحدث المطلوب إنه في مواطن محددة، يعني مثلا + +434 +00:32:30,620 --> 00:32:34,760 +شفتوا الإرتباط اللي صار بين الاستفيلو كوكاس اورياس + +435 +00:32:34,760 --> 00:32:36,160 +وبين ال antibody؟ + +436 +00:32:41,110 --> 00:32:46,650 +إذا صار طباط طبيعي لكل من ال IGG وال IGM، ال IGG + +437 +00:32:46,650 --> 00:32:49,790 +وال IGM بيعملوا activation ل ال complement لما + +438 +00:32:49,790 --> 00:32:53,610 +تصير activation ل ال complement، بيتكون في المحصلة + +439 +00:32:53,610 --> 00:32:58,230 +ال activation مُركّب، مُعقد، بيسموه membrane + +440 +00:32:58,230 --> 00:33:02,810 +attack complex، هذا بلزق فيه الخلية البكتيرية + +441 +00:33:02,810 --> 00:33:08,660 +وبعمل فيها حفرة، حفرةوبالتالي بيصير فيها إنها + +442 +00:33:08,660 --> 00:33:13,820 +محتويات الخلية إلى الخارج، بتموت البكتيريا، طيب + +443 +00:33:13,820 --> 00:33:17,180 +هذا ال .. هذا ال .. ال .. ال .. ال activation صار + +444 +00:33:17,180 --> 00:33:20,760 +في خلية جنب الكيلية أو في الكيلية، هيدمر مين؟ + +445 +00:33:20,760 --> 00:33:24,580 +هيدمر ال nephrons، صح ولا لأ؟ صار في خلايا في ال + +446 +00:33:24,580 --> 00:33:28,040 +blood vessels، هيدمر خلايا ال epithelial cells، + +447 +00:33:28,040 --> 00:33:30,560 +يفجر ال activation لل complement في غير موضوعها + +448 +00:33:30,560 --> 00:33:32,380 +تأذمين لواصل + +449 +00:33:35,400 --> 00:33:40,180 +نجي لحاجة اسمها intracellular pathogenesis تخيلوا + +450 +00:33:40,180 --> 00:33:45,020 +هذا الموضوع كده أخدت مننا يعني حجم معلومات لو لأنه + +451 +00:33:45,020 --> 00:33:50,340 +مهم وبالنسبة إنه بنعتبره يعني basic knowledge كان + +452 +00:33:50,340 --> 00:33:55,710 +احنا مركزنا عليه كتيراحنا درسنا two bacterias + +453 +00:33:55,710 --> 00:34:00,670 +بالجمع هذه ال bacterias اللي هم Chlamydia and + +454 +00:34:00,670 --> 00:34:05,310 +Rickettsia وسمناهم obligate intracellular bacteria + +455 +00:34:05,310 --> 00:34:09,750 +ايش يعني obligate intracellular يعني اصلا مابقدروش + +456 +00:34:09,750 --> 00:34:14,650 +يعيشوا free عكسها free living عكس ال obligate free + +457 +00:34:14,650 --> 00:34:19,030 +living خدوا بالكممن المميز بين obligate + +458 +00:34:19,030 --> 00:34:22,370 +intracellular وبين facultative intracellular + +459 +00:34:22,370 --> 00:34:25,750 +facultative يعني بتقدر تعيش معايشة حرة وبتقدر تعيش + +460 +00:34:25,750 --> 00:34:33,050 +معايشة .. طب تخيلوا اتنين بيعيشوا داخل خلايانة + +461 +00:34:33,050 --> 00:34:38,270 +معنى الحكي عندهم المقدرة على الهروب من عملية القتل + +462 +00:34:38,270 --> 00:34:40,990 +اللي احنا بسميها intracellular + +463 +00:34:43,720 --> 00:34:47,100 +احنا برضه يعني ال background اللي موجودة عند + +464 +00:34:47,100 --> 00:34:51,360 +المجموعة مش كلهم واحدة فمضطرين ننزل لبعض ال .. ال + +465 +00:34:51,360 --> 00:34:56,240 +.. المعلومات إلى مستوى عقلي فاكرين ال phagocyte + +466 +00:34:56,240 --> 00:34:59,500 +اللي بيقول إن أنا بتلتقم .. التقمت البكتيريا؟ + +467 +00:34:59,500 --> 00:35:02,440 +التقمتها إيش عملت فيها؟ بس أخدتها من برا و أخدتها + +468 +00:35:02,440 --> 00:35:06,020 +جوا؟ أولا طبعا مش أدفع تأثيراتك الآن بتحطها هي في + +469 +00:35:06,020 --> 00:35:09,700 +physical جوا و زي العجز ميروتكو بتصب عليها بعض + +470 +00:35:09,700 --> 00:35:14,860 +الانزيمات تمام؟ الهاضم والمحللة و بتكسرهاوإذا كانت + +471 +00:35:14,860 --> 00:35:18,360 +macro phage ال macro phage هي الرجل الحكيم أو ال + +472 +00:35:18,360 --> 00:35:22,680 +wise man لل immune system تختار منها جطع fragments + +473 +00:35:22,680 --> 00:35:26,840 +منها و بتعملها presentation لجهاز المناعة عشان + +474 +00:35:26,840 --> 00:35:29,840 +جهاز المناعة يكون لها جسام مضاد سموها antigen + +475 +00:35:29,840 --> 00:35:32,320 +presenting cell ان شاء الله لما ندرس المناعة بإذن + +476 +00:35:32,320 --> 00:35:35,360 +الله هدرس المعلومة لكن لا بأس إنه يكون عندنا بعض + +477 +00:35:35,360 --> 00:35:40,560 +المصطلحات كده و انا وصلنا احنا وصلنا إن ال + +478 +00:35:40,560 --> 00:35:47,240 +phagocyteأخدت البكتيريا على أي أساس على أساس أنه + +479 +00:35:47,240 --> 00:35:55,600 +تقتلها إذا في عملية نية مسبقة للقتل مع صدق الإصرار + +480 +00:35:55,600 --> 00:36:02,620 +والترصد طيب هناك بعض البكتيريا بعض البكتيريا عندها + +481 +00:36:02,620 --> 00:36:08,760 +المقدرة أن تدخل داخل الخلايا وتتلافى هذا التعمد في + +482 +00:36:08,760 --> 00:36:10,600 +القتل يبقى بيكون عندها + +483 +00:36:16,610 --> 00:36:21,670 +الخلايا تبقى حقيقية كيف؟ some bacteria live and + +484 +00:36:21,670 --> 00:36:24,050 +grow within polymorphonuclear cells طبعا ال + +485 +00:36:24,050 --> 00:36:27,770 +polymorphonuclear cells لاسم تاني PMNS وهي ال + +486 +00:36:27,770 --> 00:36:31,650 +neutrophil وهي الفاجوسايت يعني ال + +487 +00:36:31,650 --> 00:36:36,070 +polymorphonuclear cells هي نفسها الفاجوسايت اسم + +488 +00:36:36,070 --> 00:36:38,090 +تاني لمين؟ ل neutrophils + +489 +00:36:40,350 --> 00:36:44,970 +macrophages or monocytes by وحد avoiding entry + +490 +00:36:44,970 --> 00:36:48,290 +into phagolysis هون طيب بعد أذن الأستاذ خالد إذا + +491 +00:36:48,290 --> 00:36:54,070 +في كلام بس اللي بتبقى سبورا احنا اتفقنا إنه ال + +492 +00:36:54,070 --> 00:37:03,490 +phagocytes أخدت البتيريا عبر extension لزي سودو + +493 +00:37:03,490 --> 00:37:07,290 +بوديا بعد إن سكرتهم هدولة و بالآخر البتيريا صارت + +494 +00:37:07,290 --> 00:37:16,550 +بهذا الشكلهذا الجزء اللي زمناه فاقوزوم فاقوزوم + +495 +00:37:16,550 --> 00:37:23,490 +لأن هو الجسم اللي صار فيه الخلية البكتيريا المنوي + +496 +00:37:23,490 --> 00:37:28,950 +قتله بيجعلنا حادث منها من جوا الخلية اللايسوزوم + +497 +00:37:28,950 --> 00:37:32,790 +اللايسوزوم + +498 +00:37:32,790 --> 00:37:36,330 +يفترض في ال normal killing أنه يرتبط في الفاقوزوم + +499 +00:37:36,950 --> 00:37:43,210 +وبتكون عندنا ما يسمى ب Fago Lysosome يعني خلط ما + +500 +00:37:43,210 --> 00:37:47,810 +بين two particles اللي هم الفاجوزوم والليسوزوم، + +501 +00:37:47,810 --> 00:37:54,050 +بعدين حضرة الليسوزوم، ولحظوا الليسوزوم لن لن يفرغ + +502 +00:37:54,050 --> 00:37:58,330 +محتوياته في الخلية بشكل حر لأنه هيدمر الخلية، + +503 +00:37:58,330 --> 00:38:02,470 +بيفرغها فقط في حاجة إلها من ال brain داخل الخلية، + +504 +00:38:02,470 --> 00:38:05,730 +زي مين؟ زي الفاجو، بالظبط زي الفاجوزومالان + +505 +00:38:05,730 --> 00:38:10,850 +البكتيريا تبدأ ايش؟ تتكسر و تتحلل و كما قلت لك اذا + +506 +00:38:10,850 --> 00:38:14,130 +كانت مكروه فارغ بيصير لها presentation الاخر، الان + +507 +00:38:14,130 --> 00:38:21,170 +كيف ممكن بكتيريا تعيش جوا؟ + +508 +00:38:21,170 --> 00:38:27,050 +هاي الفالجوزوم صار جوا، الخطب تنشر الآن، احنا قلنا + +509 +00:38:27,050 --> 00:38:31,130 +ان الفالجوزوم يرتبط، في بكتيريا بتنتج chemical و + +510 +00:38:31,130 --> 00:38:34,270 +هي جوازي ال chemicals اللي انتجتها البكتيريا و + +511 +00:38:34,270 --> 00:38:40,050 +جالتها الفاجوسايت فالس أرم ارجع تمنع الفاجو أو + +512 +00:38:40,050 --> 00:38:45,010 +تمنع الليزوم من انه مش انه يرتبط انه ييجع المنطقة + +513 +00:38:45,010 --> 00:38:50,990 +أساسا تمام؟ بتكسرله الطريق تبعته ممنوع بكتيريا + +514 +00:38:50,990 --> 00:38:56,770 +أخرى بكتيريا أخرى ممكن تسمح للليزوم يرتبط لكن + +515 +00:38:56,770 --> 00:39:01,790 +تسمحلهش ان يعمل fusion no fusion يعني مافيش ايه؟ + +516 +00:39:01,830 --> 00:39:06,870 +عملية ابتحاد ..عملية ابتحاد وفيه بكتيريا are + +517 +00:39:06,870 --> 00:39:12,370 +resistant .. resistant to lysosomal destruction + +518 +00:39:12,370 --> 00:39:18,310 +يعني بتصب عليها الانزينات وكأنه بتصب عليها حاجة + +519 +00:39:18,310 --> 00:39:21,630 +يالمؤذية، عاج، تمام؟ بتهيأنا من دلوقتي، إذا في + +520 +00:39:21,630 --> 00:39:25,890 +مجموعة منها .. من .. وفي بنات كمان بكتيريا ذكية، + +521 +00:39:25,890 --> 00:39:29,710 +إيش بتعمل؟ وهي .. و هي جوا، قبل ما ال lysosome + +522 +00:39:29,710 --> 00:39:30,910 +ييجي بتحلل + +523 +00:39:33,600 --> 00:39:39,200 +الفاجوزوم إذا حلت الفاجوزوم هالحضرته من الغباء من + +524 +00:39:39,200 --> 00:39:43,700 +الغباء أنه يكف محتوياته على البكتيريا داخل الخلية + +525 +00:39:43,700 --> 00:39:49,440 +هيموت فخلاص بس كيف بيبطل له أي تأثير في هذه الحب + +526 +00:39:49,440 --> 00:39:55,460 +إذا فإن مجموعة من الحيال اللي ممكن تلجأ إلها + +527 +00:39:55,460 --> 00:39:59,680 +البكتيريا للبقاء حية خدوا بالكوا قد تنتمي إلى واحد + +528 +00:39:59,680 --> 00:40:04,160 +من الاتنينobligate intracellular يا إما الفكولتات + +529 +00:40:04,160 --> 00:40:10,000 +الـ Intracellular من الميكروباط اللى بتمتلك مقدرة + +530 +00:40:10,000 --> 00:40:12,380 +على Intracellular survival اللى هي ال + +531 +00:40:12,380 --> 00:40:16,900 +mycobacterium tuberculosis المسببة للمرض الخطير + +532 +00:40:16,900 --> 00:40:22,060 +المعروف عالميا باسم tuberculosis أو ال TB وكان هنا + +533 +00:40:22,060 --> 00:40:26,160 +في فلسطين منتشر بشكل كبير وكان له مستشفى خاص في + +534 +00:40:26,160 --> 00:40:29,940 +منطقة البراج في معسكر البراج سكان البراج مين من + +535 +00:40:29,940 --> 00:40:37,210 +سكان البراج؟حدش؟ في منطقة خلوية إلى الشرق أعتقد + +536 +00:40:37,210 --> 00:40:41,510 +أنها أصبحت مدرسة الآن أو إشي كان في مستشفى اسمه + +537 +00:40:41,510 --> 00:40:44,350 +مستشفى ال TB معروف أن عند الناس أن المستشفى ال TB + +538 +00:40:44,350 --> 00:40:47,290 +أو ال tuberculosis و أغلق يمكن في ال .. في أواخر + +539 +00:40:47,290 --> 00:40:51,810 +السبعينات لأنه بدأ عدد اللي إيه الحالات يقل بيتحسن + +540 +00:40:51,810 --> 00:40:55,470 +السبعينات واحدة ممكن نعرف في أواخر السبعينات احنا + +541 +00:40:55,470 --> 00:40:58,650 +ممكن نعرف؟ لأ مش جدا اللي تعرف يعني تسمع من .. من + +542 +00:40:58,650 --> 00:41:04,870 +الأجيال اللي .. طيبالـ Mycobacterium Libre اللي هو + +543 +00:41:04,870 --> 00:41:08,670 +بسببه الليبروسي .. بسبب بعض الليبروسي اللي هو + +544 +00:41:08,670 --> 00:41:11,630 +الجذام .. بعض الجذام .. برضه obligate .. هذه + +545 +00:41:11,630 --> 00:41:14,690 +obligate intracellular صحيح .. الأولى facultative + +546 +00:41:14,690 --> 00:41:19,470 +الـ Stereomonocytogen و هذه من أروع الميكروبات + +547 +00:41:19,470 --> 00:41:25,030 +اللى ممكن تشوفوها في ال .. داخل الخلايا هذه + +548 +00:41:25,030 --> 00:41:28,170 +البكتيريا عندما تنتقل من خلية إلى خلية تخرج من + +549 +00:41:28,170 --> 00:41:34,540 +الخلية الأولىتنتقل عبر جدار الخلوي ما بين التنتين + +550 +00:41:34,540 --> 00:41:38,760 +الخلية ال .. ال infected ال bacterial cells وهي + +551 +00:41:38,760 --> 00:41:43,540 +خلية أخرى مجاورا إلها تمام؟ خلية مجاورا إلها هاي + +552 +00:41:43,540 --> 00:41:51,380 +ال stereo monocytogen ال stereo متحركة لكنش بتعمل + +553 +00:41:51,380 --> 00:41:58,220 +تستغل الأكتين اللي موجود في الخلية و بتعمله + +554 +00:41:58,220 --> 00:41:59,220 +polymerization + +555 +00:42:02,630 --> 00:42:06,030 +ماذا يعني Polymerization؟ يعني هو فك فك تمام؟ + +556 +00:42:06,030 --> 00:42:09,330 +فبتملق .. دي Polymerize بسموه لإن هو بتملق + +557 +00:42:09,330 --> 00:42:15,650 +Polymerization فبتكون خيط أو خيوط من الأكتين و + +558 +00:42:15,650 --> 00:42:18,630 +الأكتين مادة هي اللي عاملة ال cytoskeleton تبع + +559 +00:42:18,630 --> 00:42:22,470 +الخلية يعني هي اللي رافعة السجف و لامدان يعني هي + +560 +00:42:22,470 --> 00:42:28,110 +أعملة الخلية ال cytoskeleton فبتروح و هي الأكتين + +561 +00:42:28,110 --> 00:42:31,450 +اللي بيعملها Polymerization بتحركها و كإنها صاروخ + +562 +00:42:31,950 --> 00:42:36,290 +فبتروح اللي هي ضاربة من الجدار تبقى الخلية بهذا + +563 +00:42:36,290 --> 00:42:42,430 +الشكل ومنتقلة لمين؟ الخلية الأخرى وبيسموا ال .. ال + +564 +00:42:42,430 --> 00:42:45,670 +.. ال mechanism actin rockets، rockets يعني + +565 +00:42:45,670 --> 00:42:49,630 +صواريخ، صواريخ من الأكتين، أنه الأكتين هو اللي + +566 +00:42:49,630 --> 00:42:52,770 +بيدفعها إلى اللي هو ال .. ال .. ال .. المنطقة + +567 +00:42:52,770 --> 00:42:57,630 +الأخرى و الخلية الأخرىوبالسبب لستريوسز و طبعا كتير + +568 +00:42:57,630 --> 00:43:00,510 +من ال .. من ال .. من ال infection زبانات احنا يعني + +569 +00:43:00,510 --> 00:43:05,690 +بنعطيها oses، تمام؟ tuberculosis، + +570 +00:43:05,690 --> 00:43:09,490 +salmonella osis، shigellosis، تمام؟ shigellosis، + +571 +00:43:09,490 --> 00:43:12,870 +فقد نعطيها اسم مرض زي مثلا ال salmonella، لأ ممكن + +572 +00:43:12,870 --> 00:43:16,750 +نقول typhoid fever، وبنفس الوقت نقول salmonella + +573 +00:43:16,750 --> 00:43:20,960 +osisزي ما شوفنا هانا هانا سنة لطيفة ايفاويفيبر + +574 +00:43:20,960 --> 00:43:24,000 +الشيكيلا ديزنتيريا اللي اعطينا الفاسيليا الفاسيليا + +575 +00:43:24,000 --> 00:43:26,840 +الديزنتيريا اليرسينيا بيستس ال plague اللي هو + +576 +00:43:26,840 --> 00:43:30,380 +الطاعون دار المرض معانا البروسيلا species اللي هو + +577 +00:43:30,380 --> 00:43:33,740 +بسبب البروسيلوسس اللي هي الحمّة الملطية و + +578 +00:43:33,740 --> 00:43:36,000 +الينجينيلا نيموفيلا اللي هو بسبب النمونيا + +579 +00:43:36,000 --> 00:43:39,280 +والريكينسيا والكلاميديا اللي انا ذكرتهم اول اش + +580 +00:43:39,280 --> 00:43:41,580 +هدولة التنتين هم الوحيدين اللي بنادوا ولعبناها + +581 +00:43:42,140 --> 00:43:46,000 +obligate, intra, cellular, بلا صحيح اللي هو ال .. + +582 +00:43:46,000 --> 00:43:48,660 +ال .. ال biochemistry ال biochemistry أستاذ مش + +583 +00:43:48,660 --> 00:43:52,400 +قلتي التانية obligated؟ اللي هم التانية؟ التانية + +584 +00:43:52,400 --> 00:43:57,380 +ال micro bacterium level هؤلاء هم ال .. ال .. + +585 +00:43:57,380 --> 00:44:00,720 +التنتين ال well proven well proven هذه لإنه ما + +586 +00:44:00,720 --> 00:44:04,520 +عزلنهاش لحد الآن فقد يكون هناك ضمن إنها تكون + +587 +00:44:04,520 --> 00:44:08,500 +obligate تمام؟ كده قولت لكن .. لكن اللي مؤكد اللي + +588 +00:44:08,500 --> 00:44:12,870 +هم ال .. اللي جيت ساوي اللي .. اللي كلمتطب نكمل ان + +589 +00:44:12,870 --> 00:44:14,950 +شاء الله المرة القادمة بيكون المحاضرة اللي بتاعة + +590 +00:44:14,950 --> 00:44:16,090 +هنا هي في ال .. + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..a28f6226a1fc4705aa14069c3e45954890e28004 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/NQosy7xELT8_raw.srt @@ -0,0 +1,2368 @@ +1 +00:00:20,790 --> 00:00:24,910 +بسم الله الرحمن الرحيم الموضوع اليوم ما زلنا فيه + +2 +00:00:24,910 --> 00:00:30,610 +او اكمال لما يتعلق بالبعض genesis of بكتيريا ويمكن + +3 +00:00:30,610 --> 00:00:33,650 +المحاضرة السابقة ركزنا فيها اكتر شي على ال + +4 +00:00:33,650 --> 00:00:37,110 +exotoxins اليوم هيكون تركيزنا اكتر على ال + +5 +00:00:37,110 --> 00:00:40,050 +endotoxin و بعدين هنعمل مقارنة بين ال exotoxin و + +6 +00:00:40,050 --> 00:00:44,030 +ال endotoxin ونشوف ايش الفروقات الرئيسية ما بينهم + +7 +00:00:44,030 --> 00:00:45,030 +بشكل ملخص + +8 +00:00:47,450 --> 00:00:51,630 +الـ Lipopolysaccharide شفناها بأكتر بأكتر من شكل + +9 +00:00:51,630 --> 00:00:56,670 +لكن في المحصلة هو مكون من ثلاث أجزاء رئيسية اللي + +10 +00:00:56,670 --> 00:01:01,830 +هي Lipid-A وال core polysaccharide بالإضافة إلى + +11 +00:01:01,830 --> 00:01:09,250 +اللي هو ال O-side chain عشان يصير release للإندو + +12 +00:01:09,250 --> 00:01:13,490 +توكسين لازم يصير فيه cell wall lyses عشان يصير فيه + +13 +00:01:13,490 --> 00:01:16,110 +cell wall lyses غالبا لازم يكون فيه death + +14 +00:01:21,420 --> 00:01:26,020 +لكن في المحصلة ان ال release تبعه يكون ناتج او + +15 +00:01:26,020 --> 00:01:31,850 +ناجم عن اللي هو ال license تبع SLWالمُركّب قلنا + +16 +00:01:31,850 --> 00:01:35,130 +عنه formaldehyde and heat resistant وحاولنا نعمل + +17 +00:01:35,130 --> 00:01:39,790 +علاقة في المصطلح أو في الجملة هذه بين المعلومة + +18 +00:01:39,790 --> 00:01:44,830 +اللي ذكرناها عن التوكسويد في الإكسدوكسين التوكسويد + +19 +00:01:44,830 --> 00:01:49,870 +أنتجناه عبر معالدة بالformaldehyde أو بالحرارة + +20 +00:01:49,870 --> 00:01:53,790 +للاكسدوكسين لكن هنا فشلنا أن نحصل على توكسويد + +21 +00:01:53,790 --> 00:01:56,430 +لأنها resistant and formaldehyde resistant + +22 +00:02:01,160 --> 00:02:04,880 +المطمئن ايضا ان ممكن لو ضفنا adjuvant اللى هو + +23 +00:02:04,880 --> 00:02:09,140 +المادة ال carrier الحامل لو ركبنا على ال pre + +24 +00:02:09,140 --> 00:02:14,410 +-antigen ممكن نزيد من ال antigenicity تبعهفي + +25 +00:02:14,410 --> 00:02:16,890 +المحصلة النهائية اتفقنا ان ال life of + +26 +00:02:16,890 --> 00:02:20,430 +polysaccharide هو poor انتجين بالمقارنة مع اللي هو + +27 +00:02:20,430 --> 00:02:24,550 +ال exotoxin اللي طبيعي تكوينه أصلا protein وهذا + +28 +00:02:24,550 --> 00:02:28,270 +السبب الرئيسي في الفرق بين الاتنين ال protein is + +29 +00:02:28,270 --> 00:02:34,230 +more antigenic than اللي هو ال polysaccharide ال + +30 +00:02:34,230 --> 00:02:40,750 +endotoxin من أهم ال effects تبعته انه بيعملنا + +31 +00:02:40,750 --> 00:02:46,840 +feverو بيسموه pyrogen طلع الرقم اللي بدأ اتكلمكوا + +32 +00:02:46,840 --> 00:02:51,800 +عنه الان واحد microgram لكل kilogram كافين انه + +33 +00:02:51,800 --> 00:02:56,680 +يعملوا حرارة عند المرض واحد micro .. نتكلم عن + +34 +00:02:56,680 --> 00:02:59,520 +microgram .. micro .. نتكلمش عن milligram .. نتكلم + +35 +00:02:59,520 --> 00:03:06,780 +عن microgram طيب هدر الفبر .. الان لو طلعنا على ال + +36 +00:03:06,780 --> 00:03:10,440 +effect التاني اللي اسمه disseminated intravascular + +37 +00:03:10,440 --> 00:03:15,580 +coagulationأو باختصار DIC Disseminated + +38 +00:03:15,580 --> 00:03:19,600 +Intravascular Coagulation يعني جلطات منتشرة في + +39 +00:03:19,600 --> 00:03:26,990 +الجهاز الدوري للإنسان في كل مكانليش بيعمل هيك هو؟ + +40 +00:03:26,990 --> 00:03:29,570 +عنده Coagulase هو؟ فاكريني اللي Coagulase سابعة + +41 +00:03:29,570 --> 00:03:32,310 +السنة في الكوكاست بس هاد ال bacteria ماعندهاش + +42 +00:03:32,310 --> 00:03:36,130 +Coagulase عارفينيش عندها؟ عندها ال endotoxin عنده + +43 +00:03:36,130 --> 00:03:40,050 +مقدرة انه يعمل activation لل clotting cascade اللي + +44 +00:03:40,050 --> 00:03:45,850 +هي عملية التجلط في الدم المفروض انه بشكل طبيعي + +45 +00:03:45,850 --> 00:03:50,810 +تموان في الجروح لكن وجود ال endotoxin بيعمل + +46 +00:03:50,810 --> 00:03:52,870 +activation اللي هتوان في الدم + +47 +00:03:57,530 --> 00:04:01,630 +concentration of one milligram per kilogram of + +48 +00:04:01,630 --> 00:04:08,110 +endotoxin is lethal at one + +49 +00:04:08,110 --> 00:04:11,290 +milligram per kilogram طبعا هذا التركيز خدوا + +50 +00:04:11,290 --> 00:04:16,890 +بقالكوا بالمقارنة مع ال exotoxin تركيز عالي جدا ال + +51 +00:04:16,890 --> 00:04:21,570 +exotoxin قد يكون التركيز أقل من ذلك ويكون قاتل و + +52 +00:04:21,570 --> 00:04:22,650 +انا ذكرتلكم مثال + +53 +00:04:30,540 --> 00:04:40,010 +البوتلونيوم كاف لقتل عشرين مليون فرقمجرد تذوق، + +54 +00:04:40,010 --> 00:04:43,770 +وعارفين كلمة تذوق؟ قولنا مش أكل، مش تناول، تذوق + +55 +00:04:43,770 --> 00:04:48,010 +طعام بيحتوي على البوتراناتوكسين كافي لقتل الإنسان، + +56 +00:04:48,010 --> 00:04:52,510 +إذن احنا okay مواحد ملي جرام تركيز قليل، لكن ال X + +57 +00:04:52,510 --> 00:04:57,830 +-Toxin أقل منه كمان، كمان ال X-Toxin أقل طيب، من + +58 +00:04:57,830 --> 00:04:59,930 +ال effects تبعته انه بيعمل non-specific + +59 +00:04:59,930 --> 00:05:02,750 +inflammation عارفينش يعني non-specific + +60 +00:05:02,750 --> 00:05:06,210 +inflammation؟ يعني كل ال immune system بين الشغل + +61 +00:05:06,210 --> 00:05:10,310 +في حالةcytokine طبعا نجد عالمين عن cytokine + +62 +00:05:10,310 --> 00:05:15,190 +release اللي هو ال chemical messengers كمان بيعمل + +63 +00:05:15,190 --> 00:05:18,410 +حاسمة compliment activation و ياريت نفهم هذا + +64 +00:05:18,410 --> 00:05:21,170 +الجزئي للبعض اللي مادرسش مناعة مابيعرفش إيش يعني + +65 +00:05:21,170 --> 00:05:26,170 +compliment فبنوضح شو يعني compliment ال compliment + +66 +00:05:26,170 --> 00:05:30,830 +عبارة عن بروتينات موجودة في الجسم بشكل inactive + +67 +00:05:30,830 --> 00:05:34,970 +إيش يعني inactive؟ يعني مثل هذا القلم بالضبط طلع + +68 +00:05:34,970 --> 00:05:40,480 +عليها القلم هذاما حد بينكر انه قلب لكن لو حاولنا + +69 +00:05:40,480 --> 00:05:45,960 +نكتب فيه بالطريقة دى مش هيكتب لأنه inactive سبب + +70 +00:05:45,960 --> 00:05:49,340 +انه inactive انه فيه لغطى لو فكنا لغطى بصير ايش هو + +71 +00:05:49,340 --> 00:05:52,180 +اكتر طبعا من رحمة الله سبحانه وتعالى فينا من الجسم + +72 +00:05:52,180 --> 00:05:59,980 +انها inactive لأنه لا تنشط الا ب specific events + +73 +00:05:59,980 --> 00:06:04,240 +غالبا لقتل البكتيريا لقتل البكتيريا تخيل + +74 +00:06:04,240 --> 00:06:10,340 +اندروكسينش بيعمل بنشطهطب الاندوكسين بده يدي حاله؟ + +75 +00:06:10,340 --> 00:06:15,500 +لأ بنشطها في مواضع مش مناسبة للدسن فبالتالي بتقتل + +76 +00:06:15,500 --> 00:06:18,500 +خلايا مين؟ خلايا الدسن فبصير destruction لخلايانا + +77 +00:06:18,500 --> 00:06:21,360 +احنا بال complement activation طب شيه ال + +78 +00:06:21,360 --> 00:06:23,500 +complement احنا ماعرفناه عشان صحيح ال complement + +79 +00:06:23,500 --> 00:06:27,780 +هي عبارة عن group of proteins غالبا بيرمزولها ب C + +80 +00:06:27,780 --> 00:06:34,940 +فهي مرتبة من C1 ل C9 يعني C1, C2, C3 و هكذا انا مش + +81 +00:06:34,940 --> 00:06:39,670 +وصفتها زرجلالما بيصير ال activation غالبا بتنقسم + +82 +00:06:39,670 --> 00:06:44,330 +الجسمين fragment A و fragment V برضه اذا بيسموه + +83 +00:06:44,330 --> 00:06:49,670 +C5A C5V مثلا على ال complement خمسة A و كل واحدة + +84 +00:06:49,670 --> 00:06:52,990 +منهم بنات اللي هي function طبعا مش مجلة مش مجلة + +85 +00:06:52,990 --> 00:06:56,510 +لان نتكلم فيهم الآن هدرسوهم ان شاء الله في المناعة + +86 +00:06:56,510 --> 00:07:01,890 +بشكل دقيق و هتعرفوا كيف بيشتغلوا و كيف بيتنشطوا + +87 +00:07:01,890 --> 00:07:13,510 +أصلابيسل ميتوجين بيحفظ الخلايا على الإنقسام يعني + +88 +00:07:13,510 --> 00:07:20,870 +وجوده مدارمين للانقسام الكل + +89 +00:07:20,870 --> 00:07:26,710 +كلونال بيسل activators أول حاجة أرفها مش يعني + +90 +00:07:26,710 --> 00:07:33,980 +كلونال كلون معناها سلالة كلون معناها سلالةايه + +91 +00:07:33,980 --> 00:07:38,280 +سلالة بتكون؟ ناشعة من ايه؟ من one single cell، + +92 +00:07:38,280 --> 00:07:43,620 +سلالة، نوع معين، فلما نقول monoclonal بنقول سلالة + +93 +00:07:43,620 --> 00:07:49,810 +واحدة، لما نقول polyclonal عد سلالةالمفروض ان في + +94 +00:07:49,810 --> 00:07:54,230 +جهة الملعقة بتقولكوا كل حاجة اسمها specific فلما + +95 +00:07:54,230 --> 00:07:57,950 +احنا بننشط بننشط سلالة معينة من ال B-cells + +96 +00:07:57,950 --> 00:08:02,030 +لإنتاجها على سبيل المثال ال antibodies تخيلوا ان + +97 +00:08:02,030 --> 00:08:07,510 +الاندوكسين بيعمل activation لمجموعة كبيرة من ال B + +98 +00:08:07,510 --> 00:08:10,850 +-cells كل واحدة منها بتنتج سلالة لو افترضنا انهم + +99 +00:08:10,850 --> 00:08:15,830 +كلهم معينين لقيام بوظيفة واحدة هل يستطيعوا الالاف + +100 +00:08:15,830 --> 00:08:20,410 +اللي طلعت تجوم بهذه الوظيفة؟ لأ مستطيعوالأن + +101 +00:08:20,410 --> 00:08:24,790 +المخصصة لنفوت كونها one group فلما سينا دي + +102 +00:08:24,790 --> 00:08:29,110 +polyclonal B cell activator و B cell mitogen يعني + +103 +00:08:29,110 --> 00:08:32,330 +ثلاث مختلفة و complement activation و cytokine + +104 +00:08:32,330 --> 00:08:37,410 +release و non specific inflammationعشان هيك بنقول + +105 +00:08:37,410 --> 00:08:42,030 +احنا و بنذكركم لما دراسنا ال X-Toxin كنا نتكلم عن + +106 +00:08:42,030 --> 00:08:46,050 +one step كنا نقول ان it inhibits the release of + +107 +00:08:46,050 --> 00:08:50,410 +acetylcholine it inhibits the release of glycine + +108 +00:08:50,410 --> 00:08:54,420 +اللي بيمنع ال acetylcholineit can degrade + +109 +00:08:54,420 --> 00:08:58,580 +ribosomal RNA therefore it will prevent protein + +110 +00:08:58,580 --> 00:09:02,260 +synthesis it will inactivate elongation factor 2 + +111 +00:09:02,260 --> 00:09:05,220 +to prevent protein synthesis كل هذا بقى عن one + +112 +00:09:05,220 --> 00:09:09,900 +single step very specific step بس في ال endotoxin + +113 +00:09:10,580 --> 00:09:13,280 +الجاني بيتكلم على non specific inflammation، + +114 +00:09:13,280 --> 00:09:16,160 +بيتكلم عن fever، عن disseminated intravascular + +115 +00:09:16,160 --> 00:09:19,920 +coagulation، عن complement activation، عن B cell + +116 +00:09:19,920 --> 00:09:23,140 +mitogenes، عن polyclonal B cell activation، يفجر + +117 +00:09:23,140 --> 00:09:28,980 +في عندي عاصفة من الأحداث تنتج عن حضرة من ال + +118 +00:09:28,980 --> 00:09:36,980 +endotoxinهذا الشكل يعني بيعطينا التداخلات المتنوعة + +119 +00:09:36,980 --> 00:09:40,240 +للاندوكسين مش هراكز عليه كتير تقريبا كله ذكر في + +120 +00:09:40,240 --> 00:09:44,200 +اللوحات اللي هو السابقة لكن اللوحة اللي أمامنا هذه + +121 +00:09:44,200 --> 00:09:48,100 +مهمة كتير لإنها بتلخص الفروقات بين الاندوكسين + +122 +00:09:48,100 --> 00:09:51,620 +والإفستوكسين وانت تبقى نركز على اللوحة بشكل مستمر + +123 +00:09:51,620 --> 00:09:56,400 +حتى ننهي منها ان شاء لحظوا أول شيء ال chemical + +124 +00:09:56,400 --> 00:10:01,710 +natureال .. ال .. ال .. ال exoxin مكوّن من ال + +125 +00:10:01,710 --> 00:10:04,950 +protein بينما ال endoxin مكوّن من إيه؟ من ال + +126 +00:10:04,950 --> 00:10:07,390 +lipopolysaccharide، رحظة ال molecular weight تبع + +127 +00:10:07,390 --> 00:10:12,530 +التنين، أقل، أقل من الأقل؟ ال lipopolysaccharide + +128 +00:10:12,530 --> 00:10:16,950 +أقل من ال protein، وعلى فكرة أحد سمات التمييز في + +129 +00:10:16,950 --> 00:10:19,250 +ال antigenات، يعني لو ننصن في ال antigenات من + +130 +00:10:19,250 --> 00:10:23,770 +strong لبور، تمام؟الchemical structure له دور كبير + +131 +00:10:23,770 --> 00:10:28,050 +في التصنيف رقم اتنين ال molecular weight فاكر لما + +132 +00:10:28,050 --> 00:10:31,890 +قلت انه ال free molecule لو ركّبناها على carrier + +133 +00:10:31,890 --> 00:10:35,830 +بزيد من ال antigenicity تبقى ال size مهم، صح؟ ال + +134 +00:10:35,830 --> 00:10:41,250 +size مهم. relationship to cell علاقة التكسين + +135 +00:10:41,250 --> 00:10:44,450 +بالخلية ال endotoxin هو integral part + +136 +00:10:56,680 --> 00:11:01,680 +الخلية ينتج بالخلية من الداخل و بعدين يخرج الخلية + +137 +00:11:01,680 --> 00:11:09,440 +خارج الخلية while alive و الخلية لسه حية denatured + +138 +00:11:09,440 --> 00:11:12,940 +by boiling هل بيصيله denaturation طبعا لأنه + +139 +00:11:12,940 --> 00:11:17,300 +protein هناكاللي هو ال .. ال .. ال exoseme بصيرله + +140 +00:11:17,300 --> 00:11:20,300 +denaturation بينما الأولاني و ذكرناها في الأوحى + +141 +00:11:20,300 --> 00:11:23,840 +السابقة أنه لا بصيرله denaturation لا بال formalin + +142 +00:11:23,840 --> 00:11:29,780 +ولا بالهرارة و لأنه formalin and heat stay ال + +143 +00:11:29,780 --> 00:11:34,140 +antigenic yes و yes بس ممكن تضيفه كلمتين بعد ال + +144 +00:11:34,140 --> 00:11:39,220 +yes الأولانية poor و بعد ال yes التانية strongاحنا + +145 +00:11:39,220 --> 00:11:41,160 +بنميز بين التانية، لإن التانية عندهم antigenist + +146 +00:11:41,160 --> 00:11:45,000 +الصحية، لكن واحدة ضعيفة و واحدة قوية، مين الضعيفة؟ + +147 +00:11:45,000 --> 00:11:50,560 +ال .. ال endodoxin ال potency، الفعالية، مين أكثر + +148 +00:11:50,560 --> 00:11:57,380 +فعالية؟ ال exodoxin و ال endodoxin اتفقنا ك .. لا + +149 +00:11:57,380 --> 00:12:01,180 +.. لا .. انتوا الآن .. ه .. ه .. بدنا نشيل ال + +150 +00:12:01,180 --> 00:12:05,300 +confusion اللي حصل هذا ال confusion اللي حصل إنه + +151 +00:12:05,300 --> 00:12:09,570 +الفعالية غير عن ال mechanism of actionالـ + +152 +00:12:09,570 --> 00:12:13,210 +mechanism of action في ال-exotoxin كانت specific، + +153 +00:12:13,210 --> 00:12:16,870 +في ال-endoxin كانت multiple، صح؟ هنا بتتكلم عن + +154 +00:12:16,870 --> 00:12:20,490 +concentration capable of doing the job، يعني + +155 +00:12:20,490 --> 00:12:25,430 +التركيز اللي بيجوم بالمهمة، مين أقل تركيز، ال + +156 +00:12:25,430 --> 00:12:29,450 +-exotoxin تركيزه أقل، فبالتالي فعليته يكون أعلى، + +157 +00:12:29,450 --> 00:12:33,730 +هاي المفهوم اللي احنا بنقصه فيه في الـpotency، ال + +158 +00:12:33,730 --> 00:12:37,970 +specificity الآن low degreeأنا قلتلكوا أنا بعمل + +159 +00:12:37,970 --> 00:12:40,710 +non-specific inflammation و بيعمل fever و بيعمل + +160 +00:12:40,710 --> 00:12:44,110 +complement activation و cascade لل .. لل .. لل .. + +161 +00:12:44,110 --> 00:12:47,170 +لل clotting factor activation وبالتالي هو بالفعل + +162 +00:12:47,170 --> 00:12:50,130 +non-specific عارفين بيذكروننا بإياش؟ كويسة ال + +163 +00:12:50,130 --> 00:12:55,230 +analogy لما اتكلمنا عن ال .. ال antibiotics و + +164 +00:12:55,230 --> 00:12:59,290 +اتكلمنا عن ال disinfectantsلما اتكلمنا عن ال + +165 +00:12:59,290 --> 00:13:03,190 +disinfectant لجينا ان ال disinfectant بيقتل عن + +166 +00:13:03,190 --> 00:13:05,870 +طريق ال protein denaturation و بيقتل عن طريق ال + +167 +00:13:05,870 --> 00:13:09,210 +DNA نفس ال disinfectant الواحد بينما ال antibiotic + +168 +00:13:09,210 --> 00:13:15,510 +بكون له one specific step فبالتالي .. فبالتالي كان + +169 +00:13:15,510 --> 00:13:17,910 +سهل على البكتيري اللي بتذكر و بيبقى تعمل + +170 +00:13:17,910 --> 00:13:23,490 +resistance لمن؟ لل .. لل antibiotic لكان صعب تعمل + +171 +00:13:23,490 --> 00:13:26,210 +resistance لمن؟ ل .. اللي هو ال disinfectant + +172 +00:13:30,990 --> 00:13:34,630 +Enzymatic activity هل يمتلك التوكسين Enzymatic + +173 +00:13:34,630 --> 00:13:38,450 +activity بالنسبة للاندوتوكسين ايش الجواب؟ No بينما + +174 +00:13:38,450 --> 00:13:41,990 +ال X-Toxin و لو رجعتوا اللوحات من المحاضرة السابقة + +175 +00:13:41,990 --> 00:13:47,730 +ختمناها ب Enzyme نسمينا هم X-Toxin فال X-Toxin هم + +176 +00:13:47,730 --> 00:13:49,030 +بالفعل هم Enzymatic + +177 +00:13:51,870 --> 00:13:55,470 +ال pyrogenicity و ال pyrogenicity قدرة الميكروب + +178 +00:13:55,470 --> 00:13:58,730 +على رفع درجة حرارة البسم يعني حرارة شوف نجروا شوية + +179 +00:13:58,730 --> 00:14:05,250 +أنه ال endoxin بيعمل بينما ال exoxin مش كان .. + +180 +00:14:05,250 --> 00:14:08,930 +مابيعملش غالب لكن هنا .. هنا حاطين احنا جملة + +181 +00:14:08,930 --> 00:14:13,210 +occasionally لإنه في بعض ال exoxin قد ترفع درجة + +182 +00:14:13,210 --> 00:14:14,130 +اللي هو الحرارة + +183 +00:14:16,730 --> 00:14:20,030 +طيب هذا التركيب العظيم في ال gram negative بكتيريا + +184 +00:14:20,030 --> 00:14:24,670 +المسمى بال Lego polysaccharide أو ال endotoxin هل + +185 +00:14:24,670 --> 00:14:27,790 +يولد لهم مقابل counterpart في ال gram positive هو + +186 +00:14:27,790 --> 00:14:31,950 +خاص في ال gram negative صح؟ لأن هل لو counterpart + +187 +00:14:31,950 --> 00:14:36,470 +في إجابة بتقول أنا أقصده ولا نعم؟ بتقول yes اللي + +188 +00:14:36,470 --> 00:14:39,710 +بيبتدوا شرايكان اللي موجود في ال gram positive may + +189 +00:14:39,710 --> 00:14:43,150 +play a role similar to that of endotoxin of gram + +190 +00:14:43,150 --> 00:14:47,270 +negative bacteriaيعني .. يعني ال .. ال gram + +191 +00:14:47,270 --> 00:14:50,390 +positive الأصل أنه مالهاش ليه و polysaccharide طيب + +192 +00:14:50,390 --> 00:14:52,870 +ليش بتطلع حرارة؟ ليش بتعمل intra vascular + +193 +00:14:52,870 --> 00:14:55,990 +coagulation؟ ليش بتعمل .. لإن .. لإن ال + +194 +00:14:55,990 --> 00:14:59,590 +peptidoglycan تبعها بيشتغل بنفس الطريقة اللي + +195 +00:14:59,590 --> 00:15:02,950 +بيشتغل فيها مين أن هو ليه و polysaccharide تبع ال + +196 +00:15:02,950 --> 00:15:06,530 +gram negative؟ طيب enzymes + +197 +00:15:08,260 --> 00:15:11,080 +أظن نزجنا احنا من المحاضرة الماضية و هنقول + +198 +00:15:11,080 --> 00:15:16,320 +hyaluridase و collagenase و elastase انزيمات كلها + +199 +00:15:16,320 --> 00:15:20,460 +بتكسر الانسجار ليها tissue degrading enzymes لكن + +200 +00:15:20,460 --> 00:15:24,620 +اللي جديد في اللوحة دي شيء رائع جدا يجب أن ننتبه + +201 +00:15:24,620 --> 00:15:28,980 +له بشكل كتير انا افهم ان البعض منكم مادرسش مناعة + +202 +00:15:28,980 --> 00:15:32,300 +ولا عنده اي خلفيه عن ال immunoglobulins ال + +203 +00:15:32,300 --> 00:15:36,630 +antibodies الأجسام المضادةوبالتالي كانوا يتحملون + +204 +00:15:36,630 --> 00:15:39,250 +اللي .. اللي عندهم خلفيه في هذا الموضوع يتحملونها + +205 +00:15:39,250 --> 00:15:44,570 +البسم بينتج خمس أنواع من الأجسام المضادة بلاخصهم + +206 +00:15:44,570 --> 00:15:48,950 +في كلمة ماجد IG .. IG immunoglobulin ال + +207 +00:15:48,950 --> 00:15:52,410 +immunoglobulin هو وصف أخر لمين لل antibody يعني + +208 +00:15:52,410 --> 00:15:57,550 +الآن اللي قدامي على اللوحة هنا IGA immunoglobulin + +209 +00:15:57,550 --> 00:16:04,400 +Aفي من .. من بدل ال A كمان في أربع في IGG و IGM و + +210 +00:16:04,400 --> 00:16:10,500 +IGE و IGD و احنا قلنا ال A الخامس و سميناهم ماجد + +211 +00:16:10,500 --> 00:16:16,100 +حلوين ال antibodies انا لو بعرف انكم مش دارسينهم + +212 +00:16:16,100 --> 00:16:18,460 +كان عملتلكوا صورة برضه للموضوع لأنه مهم بالنسبة + +213 +00:16:18,460 --> 00:16:22,400 +لنا كتير مهم الموضوع اللي بهمني في اللوحة هذه ان + +214 +00:16:22,400 --> 00:16:27,580 +كنتوا عارفوا ان ال IGAهو ليس أهمهم على الإطلاق لكن + +215 +00:16:27,580 --> 00:16:34,840 +له ميزة مهمة جدا انه soluble مش انه soluble ذائب و + +216 +00:16:34,840 --> 00:16:37,740 +مدام ذائب فبنلاجيه في اللعاب بنلاجيه على mucous + +217 +00:16:37,740 --> 00:16:43,120 +membrane فهو الدفاع او خط الدفاع الأول ل specific + +218 +00:16:43,120 --> 00:16:49,800 +الأول اللي بيكون على ال membranes طيب الآن افترض + +219 +00:16:49,800 --> 00:16:54,260 +انه في عنديimmunoglobulin A تبع streptococcus + +220 +00:16:54,260 --> 00:16:57,420 +pneumonia، و الـ streptococcus pneumonia دخلت ال + +221 +00:16:57,420 --> 00:17:01,700 +lungs، أيش بيعمل فيها المفروض؟ بيمسك فيها و + +222 +00:17:01,700 --> 00:17:07,160 +بدمرها، فحضرتها بتجاية و جاهزة معاها سلاح يكسرها + +223 +00:17:07,160 --> 00:17:12,880 +السلاحيبقى ال IGA هو السلاح تبعنا احنا، سلاح + +224 +00:17:12,880 --> 00:17:17,720 +دفاعي، هي جايبه مع سلاح هجومي ضد الدفاعي، اللي هو + +225 +00:17:17,720 --> 00:17:23,080 +IGA Protease، انزين بيكسر ال immunoglobulin، تمام؟ + +226 +00:17:23,080 --> 00:17:26,940 +لن تستطيع إن هتعمل infection في ذاك المكان أو + +227 +00:17:26,940 --> 00:17:29,300 +attachment في ذاك المكان إلا بهذا السلاح، شوفوا + +228 +00:17:29,300 --> 00:17:33,000 +مجموعة كبيرة من الميكروبات الخطيرة جدا، اللي مرض + +229 +00:17:33,000 --> 00:17:37,890 +معاكوا أكتر مرة، زي Hemophilus influenzaستيكوكاس + +230 +00:17:37,890 --> 00:17:43,530 +نيمونيا نيسيريا جونريا و نيسيريا مينيديتس لن + +231 +00:17:43,530 --> 00:17:46,630 +يستطيعوا انهم يعملوا infection إلا بهذا السلاح + +232 +00:17:46,630 --> 00:17:51,110 +اللي هو ال IGA Proteins تمام؟ ثالثة معلومتان في + +233 +00:17:51,110 --> 00:17:56,670 +اللوحة هذه ان فيها انها soluble antibodies that + +234 +00:17:56,670 --> 00:17:59,310 +will protect mucous membranes و ال mucous membrane + +235 +00:17:59,310 --> 00:18:03,770 +ليش بدنا protection خاصة فيه؟بنفترض الممتاحة من + +236 +00:18:03,770 --> 00:18:08,270 +تبع العين أو الأنف أو الفم أو الورثرة أو ال vagina + +237 +00:18:08,270 --> 00:18:12,430 +من جوا مافيش فيهم skin و احنا بنتنا انت نتمتع + +238 +00:18:12,430 --> 00:18:17,710 +بالجلد برا كحماية فعشان نحمي هذه المناطق تمام بدنا + +239 +00:18:17,710 --> 00:18:23,570 +اللي هو السلاح اللي اسمه IGA لكن البكتيريا إجتوا + +240 +00:18:23,570 --> 00:18:29,670 +معاها counter weapon يعني سلاح مضاد لهذا السلاح + +241 +00:18:31,930 --> 00:18:37,290 +Anti-phagocytic factors عوامل مضادة لل .. لل .. لل + +242 +00:18:37,290 --> 00:18:40,970 +phagocytosis برضه كمان احنا الان اخدوا بالكم نتكلم + +243 +00:18:40,970 --> 00:18:47,510 +على احنا لدينا جهاز منع انتج IGA جيبناله سلاح كسره + +244 +00:18:47,510 --> 00:18:50,950 +انا عندي في جهاز المنع phagocytes و macrophages هي + +245 +00:18:50,950 --> 00:18:55,550 +الان نتكلم على كيفية مكافحة اللي هو ال phagocytes + +246 +00:18:55,550 --> 00:19:00,460 +و ال .. ال phagocytosis و ال macrophagesفي خدعة + +247 +00:19:00,460 --> 00:19:10,580 +قديمة كان زمان واحد ماعرف يالبس على كتفه مثلا جلد + +248 +00:19:10,580 --> 00:19:16,300 +خروف و يروح يعني يدخل بين الخرفان و يتخبى لسبب ما + +249 +00:19:16,300 --> 00:19:20,650 +يتخبى ف he is unrecognizedليش unrecognized؟ لأنه + +250 +00:19:20,650 --> 00:19:24,730 +استخدم material من المجموعة فتخيلوا إنه في بكتيريا + +251 +00:19:24,730 --> 00:19:28,530 +عندها المقدرة إنها تاخد من الانجر تبعتنا و تحط على + +252 +00:19:28,530 --> 00:19:32,870 +حالها أو من ال chemicals اللي تبعتنا و تحط الدهن + +253 +00:19:32,870 --> 00:19:36,870 +حالها على الأساطر تبعها و بتصير لزق منها فهيكد + +254 +00:19:36,870 --> 00:19:42,790 +تبقى لمن؟ بالظبط زي أنا أعطيكم مثال بسيط لما أخدنا + +255 +00:19:42,790 --> 00:19:46,110 +الجلطات من الكواجوليز في الستفيل الكوكاس أول + +256 +00:19:46,110 --> 00:19:50,710 +مناسبة هحطكم بقى فيديو على الفيسبوكاسمه .. اسمه + +257 +00:19:50,710 --> 00:19:55,770 +الفيديو Virulence Factor لـ Staphylococcus aureus + +258 +00:19:55,770 --> 00:19:58,570 +و أنا بعتقد أنه لازم تشوفوه كلكوا لإنه بيوضّيكوا + +259 +00:19:58,570 --> 00:20:02,410 +ال .. ال Virulence Factor كميكايزم of action بي .. + +260 +00:20:02,410 --> 00:20:04,850 +بي .. بي .. في كل خطوة من خطوات ال infection من + +261 +00:20:04,850 --> 00:20:09,650 +دخول البكتيريا إلى ال dissemination تبعت على ال .. + +262 +00:20:09,650 --> 00:20:12,090 +ال .. الموقع تبعتنا، المجموعة ده اللي هي Essential + +263 +00:20:12,090 --> 00:20:17,170 +Medical Microbiology F أمام؟ اللي هي الفيميلطيب + +264 +00:20:17,170 --> 00:20:20,890 +النوع التاني few bacteria produces soluble factors + +265 +00:20:20,890 --> 00:20:24,830 +or toxins that inhibit chemotaxis by leukocytes + +266 +00:20:24,830 --> 00:20:31,390 +and thus evade phagocytosis الآن ال cytokines + +267 +00:20:31,390 --> 00:20:37,750 +وظيفتها الرئيسية chemical messengers خلية أصيبت + +268 +00:20:37,750 --> 00:20:40,890 +بستمر release ل cytokines ال cytokines بتقول ال + +269 +00:20:40,890 --> 00:20:43,790 +inflammatory cells ومن ضمنها ال phagocytes تعالوا + +270 +00:20:43,790 --> 00:20:47,630 +إلى هذا المكان هناك بكتيرياموجودة في المكان + +271 +00:20:47,630 --> 00:20:54,470 +البكتيريا ما عندهاش سلاح لمكافحة الفاجوسايت او + +272 +00:20:54,470 --> 00:21:00,090 +للبقاء حي جوا الفاجوسايت ايش بتعمل؟ بتنتج chemical + +273 +00:21:00,090 --> 00:21:05,630 +هذا ال chemical بيفهم الفاجوسايت ان هذا false + +274 +00:21:05,630 --> 00:21:11,790 +alarm يعني انضار خاطر انضار خاطر صعب حالة بترجع + +275 +00:21:11,790 --> 00:21:17,610 +الفاجوسايتلغة Chemicals بنبعت لبعض نخترخ جوّالات + +276 +00:21:17,610 --> 00:21:20,630 +بعض و بنبعت لبعض مزيجيز مضادة واحد بيقولي تعالي و + +277 +00:21:20,630 --> 00:21:26,210 +انا بقوله ياه ماتيجيش فالبكتيريا بتنتج Chemicals + +278 +00:21:26,210 --> 00:21:29,630 +to prevent the chemotactic activity of اللي هو + +279 +00:21:29,630 --> 00:21:35,750 +الخلايا تبعها لكن تعالوا نطلع على اللوحة هذه اللي + +280 +00:21:35,750 --> 00:21:40,830 +فيها معلومات ضخمة و كثيرة عن عوامل محددة تستخدم ك + +281 +00:21:40,830 --> 00:21:42,190 +antifagocytic + +282 +00:21:44,000 --> 00:21:47,180 +درست واحدة منها مهمة جدا اللي هي ال capsule، اعتقد + +283 +00:21:47,180 --> 00:21:51,500 +انه كل كوب عرفت ان الكابسول أحد مهمها كان لها + +284 +00:21:51,500 --> 00:21:55,840 +وظيفته لأي سيطرة، مظبوط، أشوش هما antifagocytosis + +285 +00:21:55,840 --> 00:22:00,640 +و التانية attachment، و يوم ما جولنا عكس بعض + +286 +00:22:00,640 --> 00:22:05,720 +التنتين صح؟ طيب ال polysaccharide capsule of + +287 +00:22:05,720 --> 00:22:10,580 +streptococcus pneumoniaHemophilus تقول أسمها بدأت + +288 +00:22:10,580 --> 00:22:13,380 +تتكرر معانا و صارت familiar الأسمها Hemophilus + +289 +00:22:13,380 --> 00:22:19,080 +influenza Triponema palidum Bacillus anthracis اه + +290 +00:22:19,080 --> 00:22:22,560 +في غلطة كبيرة هنصحيها حطوا خط تحت Bacillus + +291 +00:22:22,560 --> 00:22:27,300 +anthracis و اكتبوا عليها exception مش + +292 +00:22:27,300 --> 00:22:31,160 +polysaccharide هي مش polysaccharide هي polypeptide + +293 +00:22:31,160 --> 00:22:32,400 +polypeptide + +294 +00:22:36,750 --> 00:22:39,170 +and Klebsiella pneumonia بعرف في المعمل اللي + +295 +00:22:39,170 --> 00:22:44,510 +بتعملته capsule stain in the ink شفته طبعا أكيد + +296 +00:22:44,510 --> 00:22:46,450 +البكتيريا اللي جابولكوا إياها جابولكوا اللي هو ال + +297 +00:22:46,450 --> 00:22:49,810 +Klebsiella جابولكوا ال Klebsiella وشفته مصطلعات ال + +298 +00:22:49,810 --> 00:22:51,210 +Klebsiella ال large + +299 +00:22:53,540 --> 00:22:57,560 +Large و Mucoid عارفين إيش بيسموا ال .. ال .. ال .. + +300 +00:22:57,560 --> 00:23:01,980 +ال polysaccharide المكون للكبسول بعض الناس بيسموه + +301 +00:23:01,980 --> 00:23:05,760 +Mycopolysaccharide ليش؟ لأنه ال .. ال large + +302 +00:23:05,760 --> 00:23:10,200 +quantity منه ميوكايد و كلمة ميوكايد لزج صح؟ و + +303 +00:23:10,200 --> 00:23:15,480 +النزوج عامل بيساعد بإنه على عدم قدرة التساق الفاجو + +304 +00:23:15,480 --> 00:23:19,380 +ساعد بيه البكر المهم إن الجملة هذه اللي مارين + +305 +00:23:19,380 --> 00:23:23,260 +عليها المرسلية محتوية على معلومات خطيرة كثيرةمن + +306 +00:23:23,260 --> 00:23:27,520 +ضمنها أسماء لمجموعة من الميكروبات كلها بتحمل على + +307 +00:23:27,520 --> 00:23:30,560 +ظهرها اللي هي E-capsule فبنكون عارفين برضه انها + +308 +00:23:30,560 --> 00:23:36,800 +Decapsulated microorganism في تراكيب خاصة protein + +309 +00:23:36,800 --> 00:23:40,860 +M أو M-protein و الفمبريا في ال Streptococcus + +310 +00:23:40,860 --> 00:23:43,560 +biogenes اللي بتنتمي إلى مجموعة بيسميها group A + +311 +00:23:43,560 --> 00:23:46,200 +streptococci هدولة بيساعدوها على الغروب برضه من + +312 +00:23:46,200 --> 00:23:51,740 +الفاجوه سايتوسPseudomonas aeruginosa رقم تلاتة + +313 +00:23:51,740 --> 00:23:56,380 +Pseudomonas aeruginosa بتعمل biofilm اعتقد ان انا + +314 +00:23:56,380 --> 00:24:00,100 +مرة يعني .. بس انا حابب ان اؤكد على ان ال biofilm + +315 +00:24:00,100 --> 00:24:06,400 +هو عبارة عن ال aggregation لال micro organism و + +316 +00:24:06,400 --> 00:24:10,680 +اؤكد على حقيقة ان تصرف البكتيريا ك biofilm بيختلف + +317 +00:24:10,680 --> 00:24:18,350 +عن تصرفها ك single microorganism اش يعني بيختلف؟لو + +318 +00:24:18,350 --> 00:24:23,390 +أنا عملت اختبار شفوي لحضاراتكم وكل طالب دخل علي + +319 +00:24:23,390 --> 00:24:28,590 +المكتب وسألتها سؤال أكيد فرصتها فيه لإن الجاوب صح + +320 +00:24:28,590 --> 00:24:34,270 +بتكون أقل من فرصة لو هي اتشاورت مع زميلة إلها ولو + +321 +00:24:34,270 --> 00:24:38,070 +اتشاورت مع كل الفصل بتزيد فرص انك تحصلوا إجابة إيه + +322 +00:24:38,070 --> 00:24:40,730 +طبعا أنا ماتشجعش الأسلوب هذا في الامتحانات لو + +323 +00:24:40,730 --> 00:24:44,760 +اتشاور مع بعضلكن في الآخر هذا الواقع اللي بيصير لو + +324 +00:24:44,760 --> 00:24:48,940 +احنا بدنا نطلع برأي معين في اجتماع اكيد الرأي + +325 +00:24:48,940 --> 00:24:53,220 +بيكون افضل اما يكون اكترالبكتيريا بلات لما بتكون + +326 +00:24:53,220 --> 00:24:56,760 +مكونة biofilm تتعاملش ك single cells، بتتعامل ك + +327 +00:24:56,760 --> 00:25:00,660 +multicellular organism، يعني ك .. ك .. ك .. ك .. + +328 +00:25:00,660 --> 00:25:05,860 +كائن ذو خلايا متعددة، يعني زي ده، إلا دماغ و إلا + +329 +00:25:05,860 --> 00:25:10,040 +عينين و إلا إيدين، فبتتصرف بهذا ال .. بهذا السلوك، + +330 +00:25:10,040 --> 00:25:13,300 +وهذا التصرف بهذا السلوك بيؤدي إلى أن أدائها بيكون + +331 +00:25:13,300 --> 00:25:17,460 +أحسنإيش نقلدها أحسن؟ ال survival تبعها في ال + +332 +00:25:17,460 --> 00:25:21,800 +environment بيكون أفضل، مقاومتها للعوامل الجارحة، + +333 +00:25:21,800 --> 00:25:24,180 +يعني ال injurious agents، ال chemicals أو الحرارة، + +334 +00:25:24,180 --> 00:25:28,560 +بتكون أفضل من مما لو كانت single cell فحتى + +335 +00:25:28,560 --> 00:25:32,120 +مقاومتها لل phagocytosis لإنها تنتج المواد اللي + +336 +00:25:32,120 --> 00:25:36,100 +بتستخدمها لإنها تربط حالة بعض، برضه عبارة عن + +337 +00:25:36,100 --> 00:25:39,830 +polysaccharideوال polysaccharide برضه بيكون ميكويد + +338 +00:25:39,830 --> 00:25:44,510 +فبسهل أو بصعب عملية الفاجو أفوان سايتوس وبسهل + +339 +00:25:44,510 --> 00:25:49,510 +عملية الهروب بتتذكروا الليبو بوليساكرايد كان مكوّن + +340 +00:25:49,510 --> 00:25:52,650 +من ال lipid A core polysaccharide O sided chain ال + +341 +00:25:52,650 --> 00:25:55,730 +O sided chain على سبيل المثال في الليبو + +342 +00:25:55,730 --> 00:25:58,990 +بوليساكرايد لبعض ال gram negative بكتيريا برضه + +343 +00:25:58,990 --> 00:26:02,670 +عامل مساعد على الهروب من الفاجو سايتوس زي + +344 +00:26:02,670 --> 00:26:06,740 +الاشريشيا coli ما هو ذكره عنهافي K أنتجين و K + +345 +00:26:06,740 --> 00:26:12,060 +أنتجين هو حقيقة كابسولر أنتجين ويله مقابل في + +346 +00:26:12,060 --> 00:26:16,580 +السلمونين اللتيفي مسمى مشهور اسمه V-I أنتجين يبقى + +347 +00:26:16,580 --> 00:26:21,740 +ال K و ال V-I بيشبهوا بعض و كلاهما تعبير عن كابسول + +348 +00:26:21,740 --> 00:26:25,300 +بس صغيرة micro capsule + +349 +00:26:26,890 --> 00:26:30,750 +برضه بيساعد على الهروب طبعا شوفوا التفاصيل و + +350 +00:26:30,750 --> 00:26:35,770 +الكلمات الفطفاضة و اللي بتمر معنا مرور سريع بكرا + +351 +00:26:35,770 --> 00:26:39,670 +هتتذكرها هبتعطيكوا مثال صغير لان عشان في ال + +352 +00:26:39,670 --> 00:26:44,380 +diagnostic هتسمعوا الجملة دي اللي حاكلكواالإشريشيا + +353 +00:26:44,380 --> 00:26:48,300 +كولاي normal flora of the intestine لكن number one + +354 +00:26:48,300 --> 00:26:51,340 +cause of urinary tract infection البعض منها ممكن + +355 +00:26:51,340 --> 00:26:54,380 +يعمل neonatal meningitis إيش هي neonatal + +356 +00:26:54,380 --> 00:26:59,860 +meningitis؟ التهاب، سحاية عند الأطفال، حدثه + +357 +00:26:59,860 --> 00:27:06,740 +الولادة ويردوا إن كل العزلات كل العزلات اللي + +358 +00:27:07,440 --> 00:27:10,560 +بيعزلوها من الأطفال اللي عندهم neonatal meningitis + +359 +00:27:10,560 --> 00:27:14,220 +وردوا أن ال equalize السلالة يعني بتنتج K capsule + +360 +00:27:14,220 --> 00:27:17,760 +يبقى ال K capsule هو اللي أهلها تعمل neonatal + +361 +00:27:17,760 --> 00:27:21,920 +meningitis وسط صورتها one single factor ووجد في + +362 +00:27:21,920 --> 00:27:25,440 +البكتيريا أعطاها قدرة أكتر منها من زملاتها الأخرى + +363 +00:27:26,640 --> 00:27:31,180 +رقم ستة هو الرائع والجميل الحلو كتير اللي فيه فكرة + +364 +00:27:31,180 --> 00:27:36,680 +وفيه يعني إعجاز حقيقة نطلع على ال antibody اللي + +365 +00:27:36,680 --> 00:27:40,860 +احنا شفناه جاب شوية ال antibody بنفترض انه بهذا + +366 +00:27:40,860 --> 00:27:44,740 +الشكل اللي antigen binding side يعني ال antibody + +367 +00:27:44,740 --> 00:27:48,520 +هذا الطرف تبعه هي ال antigen تبعه بيجي برتبط بهذا + +368 +00:27:48,520 --> 00:27:55,380 +الشكلالجهة الخلفية من الـ Antibody هذي بتشكل + +369 +00:27:55,380 --> 00:28:00,260 +ريسبتور للفاجوسايتز والماكروفايريز فالان البكتيريا + +370 +00:28:00,260 --> 00:28:04,060 +مرتبطة بهذا الشكل بالـ Antigen + +371 +00:28:12,920 --> 00:28:16,360 +مين بيجي يرتبط المفروض ال macro phage ترتبط بهذا + +372 +00:28:16,360 --> 00:28:19,020 +الجزء يعني .. يعني .. يعني كل ال macro phage و ال + +373 +00:28:19,020 --> 00:28:21,720 +phagocytes اللي هي receptors في هذا الجزء بيسموه + +374 +00:28:21,720 --> 00:28:26,040 +Fc portion فلما تلجي ترتبط فيه حتى لو البكتيريا + +375 +00:28:26,040 --> 00:28:30,820 +إليها capsule حتى لو بتهرب من أي حاجة بنام صار جسر + +376 +00:28:30,820 --> 00:28:34,180 +بينها و بين جسر اللي هو ال antibody بينها و بينها + +377 +00:28:34,180 --> 00:28:38,000 +بتلتقموا على طول فإيش ال .. إيش البكتيريا بتسوي + +378 +00:28:38,000 --> 00:28:40,680 +هذا اللي اسمها Staphylococcus aureus بتنتج protein + +379 +00:28:40,680 --> 00:28:45,340 +اسمه protein إيه؟هذا كمناك بروتيوم مش هتلاجمه، هذا + +380 +00:28:45,340 --> 00:28:47,500 +ممسك الانتبار يبقى برج طب يجلبه زيك + +381 +00:28:50,380 --> 00:28:53,640 +ويربط بنفس ال .. ال .. ال .. ال receptor تبع ال + +382 +00:28:53,640 --> 00:28:57,140 +macro phage تمام؟ و الفاج و ال size ايش بيصير فيه + +383 +00:28:57,140 --> 00:29:01,880 +فوجة معلجة هنا؟ فاضي .. فاضي .. فاضي .. فاضي .. + +384 +00:29:01,880 --> 00:29:01,960 +فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. + +385 +00:29:01,960 --> 00:29:01,960 +فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. + +386 +00:29:01,960 --> 00:29:01,960 +فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. + +387 +00:29:01,960 --> 00:29:02,140 +فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. + +388 +00:29:02,140 --> 00:29:02,240 +فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. + +389 +00:29:02,240 --> 00:29:05,120 +فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. فاضي .. + +390 +00:29:05,120 --> 00:29:17,860 +فاضي .. فاضي .. فاضي .. فاضي + +391 +00:29:17,860 --> 00:29:22,770 +..الارتباط ال normal لل antigen أو لل antibody هو + +392 +00:29:22,770 --> 00:29:26,270 +عبر ال antigen binding site بهذا الشكل و على الطرف + +393 +00:29:26,270 --> 00:29:30,930 +التاني بيشكل receptor لمين؟ للماكروفاج ال protein + +394 +00:29:30,930 --> 00:29:37,210 +A بياخد ال FC portion هذا و بيرتبط فيه و بيخلي هذا + +395 +00:29:37,210 --> 00:29:41,630 +periphery وبالتالي لن يرتبط الماكروفاج أو الفاجو + +396 +00:29:41,630 --> 00:29:46,710 +صحيحإذا هذه حركة ذكية من ال Staphylococcus aureus + +397 +00:29:46,710 --> 00:29:51,410 +بإنها تعمل neutralization لل antibody اللي ممكن + +398 +00:29:51,410 --> 00:29:56,870 +يسهل في عمل اللي هو الفاجو سايتاسيس أعتقد أنه + +399 +00:29:56,870 --> 00:30:00,730 +أجهزت نفسي و أنا بشرح و أحاول بالجلم و هيفين صورة + +400 +00:30:00,730 --> 00:30:03,910 +على الصبورة فكويس أني أطلع عليها هاي + +401 +00:30:03,910 --> 00:30:09,210 +Staphylococcus مثلا Epidulis و هذه Aureus التانية + +402 +00:30:09,930 --> 00:30:14,330 +لاحظوا ال E-Dermatitis طبعا ماعندهاش protein A + +403 +00:30:14,330 --> 00:30:18,870 +فبالتالي هي اللي هو ال .. ال .. ال .. ال antibody + +404 +00:30:18,870 --> 00:30:22,830 +مرتبط بال antigen مرتبط بال antigen هنا وهي ال + +405 +00:30:22,830 --> 00:30:26,390 +phagocytes المفروض مرتبط بمين لها؟ بال Fc .. في ال + +406 +00:30:26,390 --> 00:30:29,850 +antibody في ال antibody فبتكوّن un dessert منهم + +407 +00:30:29,850 --> 00:30:32,990 +طلعوا لنا في الصورة هذه بتكوّن هل ال macro phage + +408 +00:30:32,990 --> 00:30:36,570 +هي ال dessert؟وهي ال antibody طبعا ال antibody هذا + +409 +00:30:36,570 --> 00:30:40,590 +ثنائي الشعبة اللي مابيعرفوش في خماس الشعبة كمان بس + +410 +00:30:40,590 --> 00:30:44,310 +هذا ثنائي الشعبة او يعني two antigen binding sites + +411 +00:30:44,310 --> 00:30:49,350 +ليش صار فندي هنا ال macrophage مسكت اتنين بكتيريا + +412 +00:30:49,350 --> 00:30:53,530 +عبر one single antibody ع طول الأقعد لو كان لهم + +413 +00:30:53,530 --> 00:30:55,950 +capsule بقى و مش capsule اللي هم ايش ما كان لهم + +414 +00:30:55,950 --> 00:30:59,790 +كان ساقونهم قرروا تنفيب انهم ليش لقاعد حادث الخطوة + +415 +00:30:59,790 --> 00:31:01,850 +الأولى اللي هي ال attachment ال attachment جدا مهم + +416 +00:31:01,850 --> 00:31:06,020 +جدا في هذا الموضوعهذه لما بكتيريا بتنتج من روتين A + +417 +00:31:06,020 --> 00:31:08,880 +يجي لبكتيريا التانية البكتيريا التانية استفد ل + +418 +00:31:08,880 --> 00:31:12,340 +Cocas Aureus ومن النوع اللي بنتج من روتين A إيش + +419 +00:31:12,340 --> 00:31:16,580 +عمل روتين A؟ روتين A أخد ال antibody و ارتبط فيه + +420 +00:31:16,580 --> 00:31:20,040 +هل له ريسيبترو الوكروف الفارغوسايتز؟ مالوش + +421 +00:31:20,040 --> 00:31:26,600 +ريسيبترو فبالتالي عمل تحييد لفارغوسايتز وعمل تحييد + +422 +00:31:26,600 --> 00:31:30,940 +ل ال macro phage من خلال ارتباطه بال up C portion + +423 +00:31:31,160 --> 00:31:33,480 +اللي هو protein A و ال protein A موجود على ال + +424 +00:31:33,480 --> 00:31:37,160 +surface تبع ال Staphylococcus aureus لذلك بنلاقي + +425 +00:31:37,160 --> 00:31:42,360 +احنا ال pathogenicity تبع ال Staphylococcus aureus + +426 +00:31:42,360 --> 00:31:45,020 +أعلى بكتير من ال pathogenicity تبع ال + +427 +00:31:45,020 --> 00:31:49,200 +Staphylococcus epigenesis و زي ما حتى كنا بقولها + +428 +00:31:49,200 --> 00:31:52,780 +non pathogenic بينما دي pathogenic + +429 +00:31:56,340 --> 00:32:00,080 +ال M-protein اللى شايفينه قدامنا هنا بيشتغل برضه + +430 +00:32:00,080 --> 00:32:05,080 +بطريقة انه يمنع اللى جونه عنه بيمنع ال .. ال .. ال + +431 +00:32:05,080 --> 00:32:11,820 +phagocytosis و + +432 +00:32:11,820 --> 00:32:15,620 +بيعمل عملي تنشيط لمين؟ لل compliment و أنا هذكركم + +433 +00:32:15,620 --> 00:32:19,840 +بموضوع ال compliment compliment مرة أخرى بنات هي + +434 +00:32:19,840 --> 00:32:25,900 +..جروبو بروتين لكن هي non-active، ال activation + +435 +00:32:25,900 --> 00:32:30,620 +تبعها بيحدث المطلوب إنه في مواطن محددة، يعني مثلا + +436 +00:32:30,620 --> 00:32:34,760 +شفتوا الإرتباط اللي صار بين الاستفيلو كوكاس اورياس + +437 +00:32:34,760 --> 00:32:36,160 +وبين ال antibody؟ + +438 +00:32:41,110 --> 00:32:46,650 +إذا صار طباط طبيعي لكل من ال IGG وال IGM، ال IGG + +439 +00:32:46,650 --> 00:32:49,790 +وال IGM بيعملوا activation ل ال complement لما + +440 +00:32:49,790 --> 00:32:53,610 +تصير activation ل ال complement، بيتكون في المحصلة + +441 +00:32:53,610 --> 00:32:58,230 +ال activation مُركّب، مُعقد، بيسموه membrane + +442 +00:32:58,230 --> 00:33:02,810 +attack complex، هذا بلزق فيه الخلية البكتيرية + +443 +00:33:02,810 --> 00:33:08,660 +وبعمل فيها حفرة، حفرةوبالتالي بيصير فيها إنها + +444 +00:33:08,660 --> 00:33:13,820 +محتويات الخلية إلى الخارج، بتموت البكتيريا، طيب + +445 +00:33:13,820 --> 00:33:17,180 +هذا ال .. هذا ال .. ال .. ال .. ال activation صار + +446 +00:33:17,180 --> 00:33:20,760 +في خلية جنب الكيلية أو في الكيلية، هيدمر مين؟ + +447 +00:33:20,760 --> 00:33:24,580 +هيدمر ال nephrons، صح ولا لأ؟ صار في خلايا في ال + +448 +00:33:24,580 --> 00:33:28,040 +blood vessels، هيدمر خلايا ال epithelial cells، + +449 +00:33:28,040 --> 00:33:30,560 +يفجر ال activation لل complement في غير موضوعها + +450 +00:33:30,560 --> 00:33:32,380 +تأذمين لواصل + +451 +00:33:35,400 --> 00:33:40,180 +نجي لحاجة اسمها intracellular pathogenesis تخيلوا + +452 +00:33:40,180 --> 00:33:45,020 +هذا الموضوع كده أخدت مننا يعني حجم معلومات لو لأنه + +453 +00:33:45,020 --> 00:33:50,340 +مهم وبالنسبة إنه بنعتبره يعني basic knowledge كان + +454 +00:33:50,340 --> 00:33:55,710 +احنا مركزنا عليه كتيراحنا درسنا two bacterias + +455 +00:33:55,710 --> 00:34:00,670 +بالجمع هذه ال bacterias اللي هم Chlamydia and + +456 +00:34:00,670 --> 00:34:05,310 +Rickettsia وسمناهم obligate intracellular bacteria + +457 +00:34:05,310 --> 00:34:09,750 +ايش يعني obligate intracellular يعني اصلا مابقدروش + +458 +00:34:09,750 --> 00:34:14,650 +يعيشوا free عكسها free living عكس ال obligate free + +459 +00:34:14,650 --> 00:34:19,030 +living خدوا بالكممن المميز بين obligate + +460 +00:34:19,030 --> 00:34:22,370 +intracellular وبين facultative intracellular + +461 +00:34:22,370 --> 00:34:25,750 +facultative يعني بتقدر تعيش معايشة حرة وبتقدر تعيش + +462 +00:34:25,750 --> 00:34:33,050 +معايشة .. طب تخيلوا اتنين بيعيشوا داخل خلايانة + +463 +00:34:33,050 --> 00:34:38,270 +معنى الحكي عندهم المقدرة على الهروب من عملية القتل + +464 +00:34:38,270 --> 00:34:40,990 +اللي احنا بسميها intracellular + +465 +00:34:43,720 --> 00:34:47,100 +احنا برضه يعني ال background اللي موجودة عند + +466 +00:34:47,100 --> 00:34:51,360 +المجموعة مش كلهم واحدة فمضطرين ننزل لبعض ال .. ال + +467 +00:34:51,360 --> 00:34:56,240 +.. المعلومات إلى مستوى عقلي فاكرين ال phagocyte + +468 +00:34:56,240 --> 00:34:59,500 +اللي بيقول إن أنا بتلتقم .. التقمت البكتيريا؟ + +469 +00:34:59,500 --> 00:35:02,440 +التقمتها إيش عملت فيها؟ بس أخدتها من برا و أخدتها + +470 +00:35:02,440 --> 00:35:06,020 +جوا؟ أولا طبعا مش أدفع تأثيراتك الآن بتحطها هي في + +471 +00:35:06,020 --> 00:35:09,700 +physical جوا و زي العجز ميروتكو بتصب عليها بعض + +472 +00:35:09,700 --> 00:35:14,860 +الانزيمات تمام؟ الهاضم والمحللة و بتكسرهاوإذا كانت + +473 +00:35:14,860 --> 00:35:18,360 +macro phage ال macro phage هي الرجل الحكيم أو ال + +474 +00:35:18,360 --> 00:35:22,680 +wise man لل immune system تختار منها جطع fragments + +475 +00:35:22,680 --> 00:35:26,840 +منها و بتعملها presentation لجهاز المناعة عشان + +476 +00:35:26,840 --> 00:35:29,840 +جهاز المناعة يكون لها جسام مضاد سموها antigen + +477 +00:35:29,840 --> 00:35:32,320 +presenting cell ان شاء الله لما ندرس المناعة بإذن + +478 +00:35:32,320 --> 00:35:35,360 +الله هدرس المعلومة لكن لا بأس إنه يكون عندنا بعض + +479 +00:35:35,360 --> 00:35:40,560 +المصطلحات كده و انا وصلنا احنا وصلنا إن ال + +480 +00:35:40,560 --> 00:35:47,240 +phagocyteأخدت البكتيريا على أي أساس على أساس أنه + +481 +00:35:47,240 --> 00:35:55,600 +تقتلها إذا في عملية نية مسبقة للقتل مع صدق الإصرار + +482 +00:35:55,600 --> 00:36:02,620 +والترصد طيب هناك بعض البكتيريا بعض البكتيريا عندها + +483 +00:36:02,620 --> 00:36:08,760 +المقدرة أن تدخل داخل الخلايا وتتلافى هذا التعمد في + +484 +00:36:08,760 --> 00:36:10,600 +القتل يبقى بيكون عندها + +485 +00:36:16,610 --> 00:36:21,670 +الخلايا تبقى حقيقية كيف؟ some bacteria live and + +486 +00:36:21,670 --> 00:36:24,050 +grow within polymorphonuclear cells طبعا ال + +487 +00:36:24,050 --> 00:36:27,770 +polymorphonuclear cells لاسم تاني PMNS وهي ال + +488 +00:36:27,770 --> 00:36:31,650 +neutrophil وهي الفاجوسايت يعني ال + +489 +00:36:31,650 --> 00:36:36,070 +polymorphonuclear cells هي نفسها الفاجوسايت اسم + +490 +00:36:36,070 --> 00:36:38,090 +تاني لمين؟ ل neutrophils + +491 +00:36:40,350 --> 00:36:44,970 +macrophages or monocytes by وحد avoiding entry + +492 +00:36:44,970 --> 00:36:48,290 +into phagolysis هون طيب بعد أذن الأستاذ خالد إذا + +493 +00:36:48,290 --> 00:36:54,070 +في كلام بس اللي بتبقى سبورا احنا اتفقنا إنه ال + +494 +00:36:54,070 --> 00:37:03,490 +phagocytes أخدت البتيريا عبر extension لزي سودو + +495 +00:37:03,490 --> 00:37:07,290 +بوديا بعد إن سكرتهم هدولة و بالآخر البتيريا صارت + +496 +00:37:07,290 --> 00:37:16,550 +بهذا الشكلهذا الجزء اللي زمناه فاقوزوم فاقوزوم + +497 +00:37:16,550 --> 00:37:23,490 +لأن هو الجسم اللي صار فيه الخلية البكتيريا المنوي + +498 +00:37:23,490 --> 00:37:28,950 +قتله بيجعلنا حادث منها من جوا الخلية اللايسوزوم + +499 +00:37:28,950 --> 00:37:32,790 +اللايسوزوم + +500 +00:37:32,790 --> 00:37:36,330 +يفترض في ال normal killing أنه يرتبط في الفاقوزوم + +501 +00:37:36,950 --> 00:37:43,210 +وبتكون عندنا ما يسمى ب Fago Lysosome يعني خلط ما + +502 +00:37:43,210 --> 00:37:47,810 +بين two particles اللي هم الفاجوزوم والليسوزوم، + +503 +00:37:47,810 --> 00:37:54,050 +بعدين حضرة الليسوزوم، ولحظوا الليسوزوم لن لن يفرغ + +504 +00:37:54,050 --> 00:37:58,330 +محتوياته في الخلية بشكل حر لأنه هيدمر الخلية، + +505 +00:37:58,330 --> 00:38:02,470 +بيفرغها فقط في حاجة إلها من ال brain داخل الخلية، + +506 +00:38:02,470 --> 00:38:05,730 +زي مين؟ زي الفاجو، بالظبط زي الفاجوزومالان + +507 +00:38:05,730 --> 00:38:10,850 +البكتيريا تبدأ ايش؟ تتكسر و تتحلل و كما قلت لك اذا + +508 +00:38:10,850 --> 00:38:14,130 +كانت مكروه فارغ بيصير لها presentation الاخر، الان + +509 +00:38:14,130 --> 00:38:21,170 +كيف ممكن بكتيريا تعيش جوا؟ + +510 +00:38:21,170 --> 00:38:27,050 +هاي الفالجوزوم صار جوا، الخطب تنشر الآن، احنا قلنا + +511 +00:38:27,050 --> 00:38:31,130 +ان الفالجوزوم يرتبط، في بكتيريا بتنتج chemical و + +512 +00:38:31,130 --> 00:38:34,270 +هي جوازي ال chemicals اللي انتجتها البكتيريا و + +513 +00:38:34,270 --> 00:38:40,050 +جالتها الفاجوسايت فالس أرم ارجع تمنع الفاجو أو + +514 +00:38:40,050 --> 00:38:45,010 +تمنع الليزوم من انه مش انه يرتبط انه ييجع المنطقة + +515 +00:38:45,010 --> 00:38:50,990 +أساسا تمام؟ بتكسرله الطريق تبعته ممنوع بكتيريا + +516 +00:38:50,990 --> 00:38:56,770 +أخرى بكتيريا أخرى ممكن تسمح للليزوم يرتبط لكن + +517 +00:38:56,770 --> 00:39:01,790 +تسمحلهش ان يعمل fusion no fusion يعني مافيش ايه؟ + +518 +00:39:01,830 --> 00:39:06,870 +عملية ابتحاد ..عملية ابتحاد وفيه بكتيريا are + +519 +00:39:06,870 --> 00:39:12,370 +resistant .. resistant to lysosomal destruction + +520 +00:39:12,370 --> 00:39:18,310 +يعني بتصب عليها الانزينات وكأنه بتصب عليها حاجة + +521 +00:39:18,310 --> 00:39:21,630 +يالمؤذية، عاج، تمام؟ بتهيأنا من دلوقتي، إذا في + +522 +00:39:21,630 --> 00:39:25,890 +مجموعة منها .. من .. وفي بنات كمان بكتيريا ذكية، + +523 +00:39:25,890 --> 00:39:29,710 +إيش بتعمل؟ وهي .. و هي جوا، قبل ما ال lysosome + +524 +00:39:29,710 --> 00:39:30,910 +ييجي بتحلل + +525 +00:39:33,600 --> 00:39:39,200 +الفاجوزوم إذا حلت الفاجوزوم هالحضرته من الغباء من + +526 +00:39:39,200 --> 00:39:43,700 +الغباء أنه يكف محتوياته على البكتيريا داخل الخلية + +527 +00:39:43,700 --> 00:39:49,440 +هيموت فخلاص بس كيف بيبطل له أي تأثير في هذه الحب + +528 +00:39:49,440 --> 00:39:55,460 +إذا فإن مجموعة من الحيال اللي ممكن تلجأ إلها + +529 +00:39:55,460 --> 00:39:59,680 +البكتيريا للبقاء حية خدوا بالكوا قد تنتمي إلى واحد + +530 +00:39:59,680 --> 00:40:04,160 +من الاتنينobligate intracellular يا إما الفكولتات + +531 +00:40:04,160 --> 00:40:10,000 +الـ Intracellular من الميكروباط اللى بتمتلك مقدرة + +532 +00:40:10,000 --> 00:40:12,380 +على Intracellular survival اللى هي ال + +533 +00:40:12,380 --> 00:40:16,900 +mycobacterium tuberculosis المسببة للمرض الخطير + +534 +00:40:16,900 --> 00:40:22,060 +المعروف عالميا باسم tuberculosis أو ال TB وكان هنا + +535 +00:40:22,060 --> 00:40:26,160 +في فلسطين منتشر بشكل كبير وكان له مستشفى خاص في + +536 +00:40:26,160 --> 00:40:29,940 +منطقة البراج في معسكر البراج سكان البراج مين من + +537 +00:40:29,940 --> 00:40:37,210 +سكان البراج؟حدش؟ في منطقة خلوية إلى الشرق أعتقد + +538 +00:40:37,210 --> 00:40:41,510 +أنها أصبحت مدرسة الآن أو إشي كان في مستشفى اسمه + +539 +00:40:41,510 --> 00:40:44,350 +مستشفى ال TB معروف أن عند الناس أن المستشفى ال TB + +540 +00:40:44,350 --> 00:40:47,290 +أو ال tuberculosis و أغلق يمكن في ال .. في أواخر + +541 +00:40:47,290 --> 00:40:51,810 +السبعينات لأنه بدأ عدد اللي إيه الحالات يقل بيتحسن + +542 +00:40:51,810 --> 00:40:55,470 +السبعينات واحدة ممكن نعرف في أواخر السبعينات احنا + +543 +00:40:55,470 --> 00:40:58,650 +ممكن نعرف؟ لأ مش جدا اللي تعرف يعني تسمع من .. من + +544 +00:40:58,650 --> 00:41:04,870 +الأجيال اللي .. طيبالـ Mycobacterium Libre اللي هو + +545 +00:41:04,870 --> 00:41:08,670 +بسببه الليبروسي .. بسبب بعض الليبروسي اللي هو + +546 +00:41:08,670 --> 00:41:11,630 +الجذام .. بعض الجذام .. برضه obligate .. هذه + +547 +00:41:11,630 --> 00:41:14,690 +obligate intracellular صحيح .. الأولى facultative + +548 +00:41:14,690 --> 00:41:19,470 +الـ Stereomonocytogen و هذه من أروع الميكروبات + +549 +00:41:19,470 --> 00:41:25,030 +اللى ممكن تشوفوها في ال .. داخل الخلايا هذه + +550 +00:41:25,030 --> 00:41:28,170 +البكتيريا عندما تنتقل من خلية إلى خلية تخرج من + +551 +00:41:28,170 --> 00:41:34,540 +الخلية الأولىتنتقل عبر جدار الخلوي ما بين التنتين + +552 +00:41:34,540 --> 00:41:38,760 +الخلية ال .. ال infected ال bacterial cells وهي + +553 +00:41:38,760 --> 00:41:43,540 +خلية أخرى مجاورا إلها تمام؟ خلية مجاورا إلها هاي + +554 +00:41:43,540 --> 00:41:51,380 +ال stereo monocytogen ال stereo متحركة لكنش بتعمل + +555 +00:41:51,380 --> 00:41:58,220 +تستغل الأكتين اللي موجود في الخلية و بتعمله + +556 +00:41:58,220 --> 00:41:59,220 +polymerization + +557 +00:42:02,630 --> 00:42:06,030 +ماذا يعني Polymerization؟ يعني هو فك فك تمام؟ + +558 +00:42:06,030 --> 00:42:09,330 +فبتملق .. دي Polymerize بسموه لإن هو بتملق + +559 +00:42:09,330 --> 00:42:15,650 +Polymerization فبتكون خيط أو خيوط من الأكتين و + +560 +00:42:15,650 --> 00:42:18,630 +الأكتين مادة هي اللي عاملة ال cytoskeleton تبع + +561 +00:42:18,630 --> 00:42:22,470 +الخلية يعني هي اللي رافعة السجف و لامدان يعني هي + +562 +00:42:22,470 --> 00:42:28,110 +أعملة الخلية ال cytoskeleton فبتروح و هي الأكتين + +563 +00:42:28,110 --> 00:42:31,450 +اللي بيعملها Polymerization بتحركها و كإنها صاروخ + +564 +00:42:31,950 --> 00:42:36,290 +فبتروح اللي هي ضاربة من الجدار تبقى الخلية بهذا + +565 +00:42:36,290 --> 00:42:42,430 +الشكل ومنتقلة لمين؟ الخلية الأخرى وبيسموا ال .. ال + +566 +00:42:42,430 --> 00:42:45,670 +.. ال mechanism actin rockets، rockets يعني + +567 +00:42:45,670 --> 00:42:49,630 +صواريخ، صواريخ من الأكتين، أنه الأكتين هو اللي + +568 +00:42:49,630 --> 00:42:52,770 +بيدفعها إلى اللي هو ال .. ال .. ال .. المنطقة + +569 +00:42:52,770 --> 00:42:57,630 +الأخرى و الخلية الأخرىوبالسبب لستريوسز و طبعا كتير + +570 +00:42:57,630 --> 00:43:00,510 +من ال .. من ال .. من ال infection زبانات احنا يعني + +571 +00:43:00,510 --> 00:43:05,690 +بنعطيها oses، تمام؟ tuberculosis، + +572 +00:43:05,690 --> 00:43:09,490 +salmonella osis، shigellosis، تمام؟ shigellosis، + +573 +00:43:09,490 --> 00:43:12,870 +فقد نعطيها اسم مرض زي مثلا ال salmonella، لأ ممكن + +574 +00:43:12,870 --> 00:43:16,750 +نقول typhoid fever، وبنفس الوقت نقول salmonella + +575 +00:43:16,750 --> 00:43:20,960 +osisزي ما شوفنا هانا هانا سنة لطيفة ايفاويفيبر + +576 +00:43:20,960 --> 00:43:24,000 +الشيكيلا ديزنتيريا اللي اعطينا الفاسيليا الفاسيليا + +577 +00:43:24,000 --> 00:43:26,840 +الديزنتيريا اليرسينيا بيستس ال plague اللي هو + +578 +00:43:26,840 --> 00:43:30,380 +الطاعون دار المرض معانا البروسيلا species اللي هو + +579 +00:43:30,380 --> 00:43:33,740 +بسبب البروسيلوسس اللي هي الحمّة الملطية و + +580 +00:43:33,740 --> 00:43:36,000 +الينجينيلا نيموفيلا اللي هو بسبب النمونيا + +581 +00:43:36,000 --> 00:43:39,280 +والريكينسيا والكلاميديا اللي انا ذكرتهم اول اش + +582 +00:43:39,280 --> 00:43:41,580 +هدولة التنتين هم الوحيدين اللي بنادوا ولعبناها + +583 +00:43:42,140 --> 00:43:46,000 +obligate, intra, cellular, بلا صحيح اللي هو ال .. + +584 +00:43:46,000 --> 00:43:48,660 +ال .. ال biochemistry ال biochemistry أستاذ مش + +585 +00:43:48,660 --> 00:43:52,400 +قلتي التانية obligated؟ اللي هم التانية؟ التانية + +586 +00:43:52,400 --> 00:43:57,380 +ال micro bacterium level هؤلاء هم ال .. ال .. + +587 +00:43:57,380 --> 00:44:00,720 +التنتين ال well proven well proven هذه لإنه ما + +588 +00:44:00,720 --> 00:44:04,520 +عزلنهاش لحد الآن فقد يكون هناك ضمن إنها تكون + +589 +00:44:04,520 --> 00:44:08,500 +obligate تمام؟ كده قولت لكن .. لكن اللي مؤكد اللي + +590 +00:44:08,500 --> 00:44:12,870 +هم ال .. اللي جيت ساوي اللي .. اللي كلمتطب نكمل ان + +591 +00:44:12,870 --> 00:44:14,950 +شاء الله المرة القادمة بيكون المحاضرة اللي بتاعة + +592 +00:44:14,950 --> 00:44:16,090 +هنا هي في ال .. + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/OeCcJ-QHmoY.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/OeCcJ-QHmoY.srt new file mode 100644 index 0000000000000000000000000000000000000000..581474ae6b0dfee9c7f53c91eeaab9318b65eab2 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/OeCcJ-QHmoY.srt @@ -0,0 +1,1675 @@ +1 +00:00:20,700 --> 00:00:23,180 +بسم الله الرحمن الرحيم استكمالا لما بدأناها + +2 +00:00:23,180 --> 00:00:26,260 +سأتكلم اليوم إن شاء الله عن ال viral proteins وعن + +3 +00:00:26,260 --> 00:00:29,880 +ال viral envelope بالإضافة إلى ال atypical virus + +4 +00:00:29,880 --> 00:00:33,860 +-like agents مثل ال viroids ومثل ال defective + +5 +00:00:33,860 --> 00:00:36,700 +viruses وال pseudovirons وال prions نبدأ بال + +6 +00:00:36,700 --> 00:00:41,960 +viral proteins طبعا نحن حاطين عنوان واسع اسمه + +7 +00:00:41,960 --> 00:00:44,240 +viral proteins يعني كل واحد منهم ممكن يأخذ منا + +8 +00:00:44,240 --> 00:00:48,740 +محاضرة لكن حابين أن نعطي بس صورة عنها عن ال + +9 +00:00:48,740 --> 00:00:53,720 +functions of viral proteins Capsid proteins ما هي + +10 +00:00:53,720 --> 00:00:57,840 +وظيفتها؟ إن ال capsid to protect .. to protect + +11 +00:00:57,840 --> 00:01:01,340 +genetic material ممتاز إذن ال .. إذن ال keys تبعها + +12 +00:01:01,340 --> 00:01:05,460 +رقم اثنين to mediate attachment يعني هي المسؤولة + +13 +00:01:05,460 --> 00:01:10,580 +عن الارتباط بالخلايا التي سيحدث لها infection بعد + +14 +00:01:10,580 --> 00:01:15,800 +ذلك طبعا هذا الارتباط عبر specific receptors هذا + +15 +00:01:15,800 --> 00:01:20,170 +ال interaction التي تمثل لبروتينات غشائها و غشاء + +16 +00:01:20,170 --> 00:01:23,790 +الذي هو السر، ليس هو المؤدي لل specificity بمعنى أن + +17 +00:01:23,790 --> 00:01:26,150 +this virus will infect this person + +18 +00:01:45,280 --> 00:01:50,040 +بقطيع horses يبقى فيه نوع من ال specificity التي + +19 +00:01:50,040 --> 00:01:54,100 +يحددها هو هذا ال protein المسؤول عن ال attachment + +20 +00:01:54,100 --> 00:01:57,480 +إذن ال outer membrane protein الذي هو وظيفته رائعة + +21 +00:01:57,480 --> 00:02:01,080 +to protect and to mediate attachment they + +22 +00:02:01,080 --> 00:02:04,460 +constitute as an important antigens ما هي ال + +23 +00:02:04,460 --> 00:02:07,580 +important antigens؟ ال antigens سنتعلمها في ال + +24 +00:02:07,580 --> 00:02:10,960 +bacteriology وكذلك في ال virology أن نستخدمها + +25 +00:02:10,960 --> 00:02:13,740 + بأكثر من طريقة، واحدة في ال vaccine لأنها فيها ال + +26 +00:02:13,740 --> 00:02:19,380 +strain variations، صحيح؟ مثل ال polio الذي هو + +27 +00:02:19,380 --> 00:02:23,980 +الفيروس الشلل، الفيروس ال polio، تمام؟ فيه ال + +28 +00:02:23,980 --> 00:02:26,580 +strain واحد واثنين وثلاثة، إذا أردنا عمل + +29 +00:02:26,580 --> 00:02:31,200 +vaccination نريد تغطية الثلاثة إذا أردنا التمييز بين + +30 +00:02:31,200 --> 00:02:34,460 +الإصابات، عذابنا للفيروس، نريد التعرف على هل هو + +31 +00:02:34,460 --> 00:02:38,520 +فيروس واحد أم تطور له؟ طيب ال immune system ليس + +32 +00:02:38,520 --> 00:02:41,020 +بالساكت، يستجيب أيضا لهذه ال .. ال .. ال .. ال .. + +33 +00:02:41,020 --> 00:02:41,500 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +34 +00:02:41,500 --> 00:02:41,580 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +35 +00:02:41,580 --> 00:02:41,860 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +36 +00:02:41,860 --> 00:02:42,700 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +37 +00:02:42,700 --> 00:02:42,840 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +38 +00:02:42,840 --> 00:02:43,700 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +39 +00:02:43,700 --> 00:02:44,620 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +40 +00:02:44,620 --> 00:02:50,140 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +41 +00:02:56,630 --> 00:03:00,030 +ضد البروتينات هذه to neutralize ماذا يعني + +42 +00:03:00,030 --> 00:03:04,110 +neutralize؟ هذا الفيروس، هذه ال attachment تبعته، + +43 +00:03:04,110 --> 00:03:07,630 +هذا البروتين، ال antibody ارتبط به، هذا ال + +44 +00:03:07,630 --> 00:03:12,110 +antibody عمل blocking للبروتين هذا، ارتبط من أين؟ من + +45 +00:03:12,110 --> 00:03:14,910 +ال receptor، وبالتالي أصبح neutralized، تحيد + +46 +00:03:14,910 --> 00:03:19,830 +الفيروس، ماذا نسميها؟ neutralizing antibodies يعني + +47 +00:03:19,830 --> 00:03:24,050 +أجسام مضادة محايدة التأثير أو قاتلة للتأثير الذي هو + +48 +00:03:24,050 --> 00:03:25,310 +هذا الفيروس + +49 +00:03:28,290 --> 00:03:32,950 +الـ internal viral proteins vary depending on the + +50 +00:03:32,950 --> 00:03:36,030 +virus، نقول إن الكلام الذي على الأوتر، خذ الكلام + +51 +00:03:36,030 --> 00:03:40,430 +الذي على الداخل، على ال internal proteins تخيلوا + +52 +00:03:40,430 --> 00:03:44,710 +فيروس ذاهب على خلية ليحدث لها infection، والمادة التي + +53 +00:03:44,710 --> 00:03:48,750 +مادة وراثته تبعته negative sense، negative sense mRNA + +54 +00:03:50,020 --> 00:03:57,020 +أو positive sense positive sense mRNA تمام لكن deployed مثل ال + +55 +00:03:57,020 --> 00:04:02,760 +HIV وال HIV له ميزة أنه يعمل integration داخل + +56 +00:04:02,760 --> 00:04:08,170 +الجينة وتبعها الفيروسينز هؤلاء ذاهبين على خلية وليس + +57 +00:04:08,170 --> 00:04:12,490 +لهم إنزيمات .. ليس لهم إنزيمات في الخلية تتعامل + +58 +00:04:12,490 --> 00:04:16,510 +معهم لكي يتكاثرون فيجب أن يحملوا الفيروس .. يحملوا + +59 +00:04:16,510 --> 00:04:21,070 +إنزيماتهم معهم، يبقى بعض ال internal proteins + +60 +00:04:21,070 --> 00:04:24,070 +تكون عبارة عن ماذا؟ عبارة عن .. عبارة عن enzymes + +61 +00:04:24,070 --> 00:04:27,990 +for the genome replication مثل reverse + +62 +00:04:27,990 --> 00:04:30,870 +transcriptase الذي يحمله ال HI فيه على سبيل + +63 +00:04:30,870 --> 00:04:35,400 +المثال وبعض ال .. ال .. الفيروسات الأخرى، في عندي + +64 +00:04:35,400 --> 00:04:39,040 +protein نسميه matrix protein فهذا ال matrix + +65 +00:04:39,040 --> 00:04:44,400 +protein هو موجود في الفيروسات التي لها envelope + +66 +00:04:44,400 --> 00:04:51,360 +وهو الذي يملأ المسافة ما بين ال capsid وما بين ال + +67 +00:04:51,360 --> 00:04:54,180 +envelope يعني ال envelope لا يكون معلق في الهواء + +68 +00:04:54,180 --> 00:04:57,280 +يكون بينه وبين ال capsid مجموعة من ال protein + +69 +00:04:57,280 --> 00:05:02,800 +نسميها matrix protein الغريب في الموضوع أنه في بعض + +70 +00:05:02,800 --> 00:05:08,040 +ال antigens التي تنتجها الفيروسات can act as a + +71 +00:05:08,040 --> 00:05:11,020 +super antigen، هل تتذكرون ال super antigen؟ ال super + +72 +00:05:11,020 --> 00:05:15,460 +antigen كان يعمل كيف؟ ب non specific activation لل + +73 +00:05:15,460 --> 00:05:20,680 +T lymphocytes من أجمل ما يكون أن نقارن أننا + +74 +00:05:20,680 --> 00:05:24,360 +نتحدث عن البكتيريا وال viruses، مثل Staphylococcus aureus + +75 +00:05:24,360 --> 00:05:28,080 +إذا تتذكرون ال toxic shock syndrome toxin، هل تتذكرونه؟ + +76 +00:05:28,080 --> 00:05:32,880 +هذا ال .. ال .. ال toxin كان .. كان عمل لنا septic + +77 +00:05:32,880 --> 00:05:37,080 +shock عن طريق أنه عمل non specific activation لل T + +78 +00:05:37,080 --> 00:05:42,120 +lymphocyte، طيب الفيروسات لماذا تعمل super antigen + +79 +00:05:42,120 --> 00:05:48,180 +مثال نقول ال Epstein-Barr virus وال + +80 +00:05:48,180 --> 00:05:52,840 +cytomegalovirus وال retroviruses أو ال retrovirus + +81 +00:05:52,840 --> 00:05:58,480 +هذه أمثلة على فيروسات تنتج ما هو super antigen + +82 +00:05:58,480 --> 00:06:02,420 +الأول والثاني الذي هو ال Epstein-Barr virus وال + +83 +00:06:02,420 --> 00:06:05,880 +cytomegalovirus وكلاهما ينتميان إلى عائلة اسمها + +84 +00:06:06,830 --> 00:06:10,390 +herpes virus يبقى ال herpes virus منها اثنان + +85 +00:06:10,390 --> 00:06:12,570 +الذي هما cytomegalovirus واللذان يعملان + +86 +00:06:12,570 --> 00:06:16,630 +بأيروفايروس وواحد من عائلة أخرى اسمه رتروفايروس + +87 +00:06:16,630 --> 00:06:21,750 +ماوس ماموري ديومارفايروس، ما هي ال hypothesis + +88 +00:06:21,750 --> 00:06:27,670 +الفرضية، نفترض أن لماذا الفيروسات نقول the + +89 +00:06:27,670 --> 00:06:31,130 +current hypothesis offered to explain why this + +90 +00:06:31,130 --> 00:06:33,650 +virus produces a super antigen + +91 +00:06:40,600 --> 00:06:48,540 +يعني تخيلوا هو يُهيئ الوسط أو البيئة لتكاثر بإنه + +92 +00:06:48,540 --> 00:06:52,600 +يعمل activation لل .. لل .. لل .. لل telemposite + +93 +00:06:52,600 --> 00:06:58,060 +بتاعة CD4 لأنّه يجد مكانًا يتكاثر فيه تمام؟ فهو + +94 +00:06:58,060 --> 00:07:03,280 +كأنه ينتج culture media لنفسه لكي يتكاثر في + +95 +00:07:03,280 --> 00:07:07,290 +داخله some viruses contains regulatory proteins وال + +96 +00:07:07,290 --> 00:07:09,230 +regulatory proteins موجودة نقول لها بشوية + +97 +00:07:09,230 --> 00:07:13,250 +matrix protein صحيح؟ بين ال .. ال .. ال capsid وبين + +98 +00:07:13,250 --> 00:07:16,930 +ال .. ال .. ال envelope هذه بين المادة الوراثية وبين + +99 +00:07:16,930 --> 00:07:20,350 +الذي هو ال .. ال .. ال capsid بين المادة + +100 +00:07:20,350 --> 00:07:22,750 +الوراثية وبين ال capsid نسميها بعض الأحيان + +101 +00:07:22,750 --> 00:07:27,510 +tegument لها .. لها هذه المنطقة tegument تمام؟ وال + +102 +00:07:27,510 --> 00:07:30,450 +located بين ال nuclear capsid وال envelope هذه + +103 +00:07:30,450 --> 00:07:35,210 +البروتينات تحتوي على تنظيم للترجمة والترجمة تحكم + +104 +00:07:35,210 --> 00:07:39,290 +أيضا فيروس أو ترجمة سلوكية يعني البروتينات هذه + +105 +00:07:39,290 --> 00:07:45,170 +لها وظائف تنظيمية من حيث الترجمة والترجمة وقد تنظم + +106 +00:07:45,170 --> 00:07:51,190 +فيها من خلالها الترجمة وترجمة للفيروسات أو للجسم + +107 +00:07:51,190 --> 00:07:56,070 +العادل للجسم العادل نفسه المثال عليها ال members ل + +108 +00:07:56,070 --> 00:07:59,810 +ال herpes virus family مثل ال herpes simplex وال + +109 +00:07:59,810 --> 00:08:04,810 +cytomegalovirus يحتويان على tegument بارزة ماذا يعني + +110 +00:08:04,810 --> 00:08:08,350 +بارزة، بروميلان بارزة and well characterized يعني + +111 +00:08:08,350 --> 00:08:11,050 +نستطيع بال electron microscope رؤيتها بشكل مميز + +112 +00:08:11,050 --> 00:08:15,810 +جدا، نأتي لل envelope وقد تحدثنا عنه صحيح + +113 +00:08:15,810 --> 00:08:19,350 +مسبقا في الكلام، عامله ببعض الكلمات البسيطة ال + +114 +00:08:19,350 --> 00:08:24,850 +envelope هو membrane يحيط ببعض الفيروسات التي نحن + +115 +00:08:24,850 --> 00:08:28,370 +نسميها envelope viruses، envelope التي ليس لها + +116 +00:08:28,370 --> 00:08:32,730 +اسمناها زمان، إما non-envelope أو naked أو what's + +117 +00:08:32,730 --> 00:08:35,490 +in term يعني nucleocapsid، nucleocapsid + +118 +00:08:40,320 --> 00:08:42,500 +طيب نحن نعرف أنه فيه advantage وفيه just advantage + +119 +00:08:42,500 --> 00:08:48,240 +لوجود ال envelope، ال advantage يسهل عملية ال + +120 +00:08:48,240 --> 00:08:52,360 +attachment وال fusion مع ال cell، وبالتحديد قطرة + +121 +00:08:52,360 --> 00:08:55,400 +زيت وقطرة زيت لو وضعناهم مع بعض، هذا على طول + +122 +00:08:55,400 --> 00:09:00,220 +يندمج ويصبح قطرة واحدة، هذه ميزة إيجابية أنّه + +123 +00:09:00,220 --> 00:09:02,860 +يُسهل عملية ال penetration أو ال attachment لل + +124 +00:09:02,860 --> 00:09:03,180 +penetration + +125 +00:09:08,390 --> 00:09:11,870 +يعمل أو يصبح it confers instability عكس ال + +126 +00:09:11,870 --> 00:09:16,780 +stability وبالتالي بيلاقي أن هذه الفيروسات هذه + +127 +00:09:16,780 --> 00:09:22,740 +الفيروسات نجدها بتنتقل عبر الـ intimate contact أما + +128 +00:09:22,740 --> 00:09:26,580 +sexual contact أو blood transfusion وبعض الأحيان + +129 +00:09:26,580 --> 00:09:29,740 +عبر الـ respiratory tract عبر الـ respiratory tract + +130 +00:09:29,740 --> 00:09:33,320 +لكن ولا واحد من الـ envelope viruses بينتقل عزيزي + +131 +00:09:33,320 --> 00:09:36,860 +مثال عبر الفكهة الـ oral عبر الفكهة الـ oral لأنه + +132 +00:09:36,860 --> 00:09:39,580 +هيمر عبر المائدة والمائدة فيها حالة دائمة + +133 +00:09:39,580 --> 00:09:43,000 +destruction للممبرينت وبالتالي الميزة اللي كانت + +134 +00:09:43,000 --> 00:09:45,700 +موجودة فيه أن الممبران بيسهلوا عملية الـ attachment + +135 +00:09:45,700 --> 00:09:49,180 +إذا راح الممبران مش هيقدر يعمل attachment أنت هنا + +136 +00:09:49,180 --> 00:09:53,220 +من الجهة يبقى الـ viral envelope بداله، يفهم أنه إله + +137 +00:09:53,220 --> 00:09:56,420 +هذا الـ advantage وإله الـ disadvantage اللي موجود + +138 +00:09:56,750 --> 00:10:00,850 +طيب في كمان نقطة أنه بيحتوي على proteins في الـ + +139 +00:10:00,850 --> 00:10:03,590 +membrane هذه الـ proteins اللي بيسميها الـ + +140 +00:10:03,590 --> 00:10:07,230 +glycoproteins أو الـ GP هذي الـ GP من ورا.. من ورا + +141 +00:10:07,230 --> 00:10:10,650 +.. من ورا المخودة مش موجودة في خلايا هذه الفيروس + +142 +00:10:10,650 --> 00:10:14,970 +بيصنعها هو داخل الخلية هو داخل الخلية وبيغرسها في + +143 +00:10:14,970 --> 00:10:18,290 +الـ cell membrane وهو طالع بياخد membrane صح؟ + +144 +00:10:18,290 --> 00:10:21,550 +بياخد membrane فبياخد الـ membrane المغروس فيه اللي + +145 +00:10:21,550 --> 00:10:21,770 +هو + +146 +00:10:28,480 --> 00:10:32,140 +هذه الـ spikes إليها وظائف مش مجرد أنه والله spikes + +147 +00:10:32,140 --> 00:10:34,920 +منظر يعني عاملينها للـ.. للفيروس، لأ إليها وظائف + +148 +00:10:34,920 --> 00:10:39,000 +من ضمنها.. من ضمنها.. من ضمنها إنها receptor + +149 +00:10:39,000 --> 00:10:44,460 +لخلايا أخرى، هي receptor لخلايا أخرى، طب في هذه + +150 +00:10:44,460 --> 00:10:48,260 +اللحظة مضطرين أن ننقل على نشوف عملية replication + +151 +00:10:48,260 --> 00:10:52,840 +الشكل اللي قدامنا أو اللوحة اللي قدامنا فيها تلت + +152 +00:10:52,840 --> 00:10:55,580 +أجزاء رئيسية، تلت أعمدة، العمود الأول attachment and + +153 +00:10:55,580 --> 00:10:58,360 +penetration، العمود الثاني synthesis والعمود + +154 +00:10:58,360 --> 00:11:02,900 +الثالث assembly and release لأن أنا هتكلم عن الـ + +155 +00:11:02,900 --> 00:11:06,420 +attachment والـ penetration نشوف هي فيروس هذا ليس + +156 +00:11:06,420 --> 00:11:11,900 +له envelope non envelope يعني هذا naked virus عمل + +157 +00:11:11,900 --> 00:11:16,560 +attachment على الـ membrane وعمل emptying للـ content + +158 +00:11:16,560 --> 00:11:19,520 +هو لسه برة زي الـ bacteriophage في الـ bacteriophage + +159 +00:11:19,520 --> 00:11:25,140 +هذا نموذج، النموذج الثاني اللي احنا بدنا نشوفه اللي + +160 +00:11:25,140 --> 00:11:29,400 +هو الـ membrane fusion لمن؟ للـ invert هذا invert هي + +161 +00:11:29,400 --> 00:11:37,320 +receptors، شوفوا الآن عملية الـ fusion ومن ثم + +162 +00:11:37,320 --> 00:11:41,460 +emptying لمن؟ للـ virus اللي هو الـ nucleocapsid إلى + +163 +00:11:41,460 --> 00:11:47,370 +داخل الخلية، الثالثة المسمّى تبعها الصحيح يعني لا + +164 +00:11:47,370 --> 00:11:51,810 +يعبّر عن ما نقوله كلمة phagocytosis، عمالية معروفة + +165 +00:11:51,810 --> 00:11:56,390 +لكن هذه تشبهها نسميها endocytosis مش phagocytosis + +166 +00:11:56,390 --> 00:12:02,410 +أو pinocytosis، المهم إنه فيه entry مش خروج للأعلى + +167 +00:12:02,410 --> 00:12:08,030 +invagination للأسفل، تشوفوا الأعلى اللقطة هذه إنه + +168 +00:12:08,030 --> 00:12:10,650 +الفيروس دخل إلى الداخل وتكون في physical زي + +169 +00:12:10,650 --> 00:12:16,010 +الفاجوزوم زي الفاجوزوم، هي طرق اللي هو إيه الـ + +170 +00:12:16,010 --> 00:12:19,450 +attachment والـ entry للفيروس، طبعا إذا بتلاحظوا + +171 +00:12:19,450 --> 00:12:25,750 +هنا في جليكو بروتين صح؟ هذه الجليكو بروتين ما بتكونش + +172 +00:12:25,750 --> 00:12:30,050 +موجودة على الخلية هذه الآن بالمنظر اللي شايفينه + +173 +00:12:30,050 --> 00:12:33,310 +قدامك وهذا هي أيضا عبارة عن receptors للفيروس هي + +174 +00:12:33,310 --> 00:12:36,950 +receptors للفيروس، لو شفنا الـ.. الـ.. الـ release + +175 +00:12:36,950 --> 00:12:41,670 +نشوف الـ release مباشرة تمام + +176 +00:12:44,030 --> 00:12:49,590 +بنقفز على الـ synthesis بعد ما نشوفه بيشتغل + +177 +00:12:49,590 --> 00:12:56,970 +هيك الـ naked virus بيتغير لما تعمل lysis لخلية و + +178 +00:12:56,970 --> 00:12:59,590 +بتطلع بتدمرها تعملها destruction، متذكروا كلمة + +179 +00:12:59,590 --> 00:13:03,750 +lysis؟ خلية دمرت من ضمن أنسجة هينتقل الفيروس + +180 +00:13:03,750 --> 00:13:06,350 +لخلايا أخرى ويدمرها فهيصير في النتيجة اللي فيها + +181 +00:13:06,350 --> 00:13:09,230 +أنها destruction، حد بيسميها cytopathic effect + +182 +00:13:09,230 --> 00:13:13,710 +نعرفها كويس للمحاضرة الجاية، يبقى صار في end release + +183 +00:13:13,710 --> 00:13:16,370 +بالـ destruction، الآن نجي للـ envelope viruses الـ + +184 +00:13:16,370 --> 00:13:20,790 +envelope viruses اللي لها ذكية قد تتكاثر وتبقى في + +185 +00:13:20,790 --> 00:13:24,470 +الخلية وتخرج من الخلية، دول ما بتدمرش هذه الخلية عن + +186 +00:13:24,470 --> 00:13:26,810 +طريق هاي الـ proteins اللي بتصنعها الـ glycoprotein + +187 +00:13:26,810 --> 00:13:34,870 +تم لها insertion في الـ cell membrane و + +188 +00:13:34,870 --> 00:13:39,210 +من ثم الفيروس بيهو خارج و بياخد يزور من الـ + +189 +00:13:39,210 --> 00:13:43,710 +membrane زي ما أنتو زي ما أنا بدي اتحركوا بياخد + +190 +00:13:43,710 --> 00:13:47,310 +جزء وبيسموه العملية body كأنه تبرع وكأنه أخد + +191 +00:13:47,310 --> 00:13:52,950 +قطعة وطلع فيها، فلكن ما صارش إيه؟ destruction لـ + +192 +00:13:52,950 --> 00:14:01,310 +الخلايا هاي + +193 +00:14:01,310 --> 00:14:06,410 +الـ glycoprotein زي ما حكينا وبترسلوها الـ GP طبعا + +194 +00:14:06,410 --> 00:14:09,150 +تختلف من virus لـ virus تركيبها وطبيعتها وبالتالي هي + +195 +00:14:09,150 --> 00:14:13,070 +محدد للانتجينات، لاحظوش صار destruction ما صارش + +196 +00:14:13,070 --> 00:14:26,470 +destruction للخلايا، تكلمنا + +197 +00:14:26,470 --> 00:14:28,190 +عن إيجابيات في الـ envelope + +198 +00:14:31,420 --> 00:14:36,480 +أو سلبية، واتكلمنا عن مكونات الـ spikes اللي هي الـ + +199 +00:14:36,480 --> 00:14:42,040 +glycoproteins اللي موجودة في الـ embryo وحكينا عن + +200 +00:14:42,040 --> 00:14:43,920 +الـ instability اللي هي الـ keyword في اللوحة اللي + +201 +00:14:43,920 --> 00:14:48,620 +شايفينها، الـ instability من وين جاية؟ كان مرة بأن الـ + +202 +00:14:48,620 --> 00:14:51,160 +membrane very sensitive بيصير له destruction و + +203 +00:14:51,160 --> 00:14:54,700 +بيصير له destruction، طبعا الـ destruction هلأ بيصير + +204 +00:14:54,700 --> 00:14:59,840 +بأي من المراكبات السهلة ومن ضمنها الجفاف.. من + +205 +00:14:59,840 --> 00:15:03,460 +ضمنها الجفاف، يعني لو الفيروس رأى على براية الـ + +206 +00:15:03,460 --> 00:15:09,040 +clinics تمام، على طول بيصير فيه dryness وبالتالي + +207 +00:15:09,040 --> 00:15:14,140 +بيجف وبتدمر الـ cell membrane، ليه؟ عشان أقولنا الـ + +208 +00:15:14,140 --> 00:15:17,620 +transmission تبعه بيكون فقط بالـ intimate contact + +209 +00:15:17,620 --> 00:15:19,880 +blood transfusion أو sexual contact أو بعض الأحيان + +210 +00:15:19,880 --> 00:15:24,810 +الـ respiratory، بقرعه في الـ environment أقل من بقاء + +211 +00:15:24,810 --> 00:15:29,290 +اللي هو الـ naked virus، تذكروا فيروس الرشح اللي + +212 +00:15:29,290 --> 00:15:32,530 +بينتقل عبر الـ respiratory تمام هو naked virus + +213 +00:15:32,530 --> 00:15:39,050 +naked virus، وقد يعني أنه صارت بالرشح، صارت بالرشح + +214 +00:15:39,050 --> 00:15:43,670 +مع أنه احنا ما حكينا بمرضى وما جفنا مرضى اصلا عندهم + +215 +00:15:43,670 --> 00:15:50,720 +common cold، والسبب أن الفيروس فيولو لمسنا + +216 +00:15:50,720 --> 00:15:54,120 +بأصابعنا أو دخّان أصابعنا في الـ conductiva أو بوصل + +217 +00:15:54,120 --> 00:16:08,120 +الأنف أو الفن بطريقة ما ممكن ينتقل لكل إنسان هنا + +218 +00:16:08,120 --> 00:16:10,880 +نفس المعلومات اللي حكيناها أن الـ.. الـ antigenات + +219 +00:16:10,880 --> 00:16:13,020 +اللي مولدة على السطح هي اللي بتشكل الـ stereotypes + +220 +00:16:13,020 --> 00:16:16,280 +هنا كلام مكرر من الكلام اللي حكيناه بالأول، نجي إلى + +221 +00:16:16,280 --> 00:16:20,000 +الجزء الأخير من الموضوع، Atypical viruses like + +222 +00:16:20,000 --> 00:16:23,160 +Asian defective viruses, pseudovirions, virioids + +223 +00:16:23,160 --> 00:16:28,020 +and priors، طبعا على الـ defective viruses نبدأ + +224 +00:16:28,020 --> 00:16:31,320 +بالـ defective viruses، الـ defective viruses هي + +225 +00:16:31,320 --> 00:16:36,960 +بالاصطلاح العامي فيروسات يعني خربانة، خربانة بمعنى + +226 +00:16:36,960 --> 00:16:42,140 +أنه ما بتقدرش تتكاثر إلا في وجود ما يسمى بالـ helper + +227 +00:16:42,140 --> 00:16:46,630 +فيروس، هنا بتطلع عليها من زاوية ثانية، زاوية فيروس + +228 +00:16:46,630 --> 00:16:50,790 +بسبب برضه إنه معروف عنه defective اسمه Hepatitis D + +229 +00:16:50,790 --> 00:16:56,810 +Hepatitis D بتاعه مع A وB وC وفيها إنه D، D هذا + +230 +00:16:56,810 --> 00:17:00,370 +مستحيل يشوفه عند بني آدم إلا لازم يكون معاه B، + +231 +00:17:00,370 --> 00:17:04,450 +لكن مش بالضرورة إنه إذا كان معاه B يكون معاه D + +232 +00:17:05,050 --> 00:17:10,150 +يبقى وجود D مرتبط بمين؟ بـ B، مش صحيح أن الـ B مش + +233 +00:17:10,150 --> 00:17:13,410 +مرتبط بيه، يعني لو أنا اليوم من الأيام حصل واحد + +234 +00:17:13,410 --> 00:17:17,050 +عن D ولقاه positive، ما أنت تحكي عنه؟ اندوبي، + +235 +00:17:17,050 --> 00:17:20,830 +without B virus they cannot replicate، they cannot + +236 +00:17:20,830 --> 00:17:25,110 +replicate، طيب هذه نقطة مهمة وكويسة في الطب أنه + +237 +00:17:25,110 --> 00:17:28,690 +نعرف أن فيه فيروسات معتمدة على فيروسات أخرى، النقطة + +238 +00:17:28,690 --> 00:17:32,390 +الثانية بدنا نستخدمها في تفسير لماذا نشفى من + +239 +00:17:32,390 --> 00:17:37,710 +الفيروسات تلقائيا بدون علاج بعض الأحيان أو بكثير + +240 +00:17:37,710 --> 00:17:41,150 +من الأحيان أو بكثير من الأمر الفيروسي، لو قلنالكوا + +241 +00:17:41,150 --> 00:17:45,090 +أن الـ defective virus هو عملية روتينية في التصنيع + +242 +00:17:45,090 --> 00:17:49,950 +العادي للفيروسات، يعني فيروس دخل خلية واتكاثر جوامش + +243 +00:17:49,950 --> 00:17:54,010 +احنا ضمن التكاثر فيها اسم assembly، تجمية، فبقول + +244 +00:17:54,010 --> 00:17:59,730 +إن عند التجمية قد يحصل.. قد يحصل خلل، فجزء من الـ + +245 +00:17:59,730 --> 00:18:03,570 +viral genome يدخل و جزء ما يدخلش، وبالتالي الفيروس + +246 +00:18:03,570 --> 00:18:08,960 +بيكون إيش الآن؟ ناقص، defective، قد يصل عدد الـ + +247 +00:18:08,960 --> 00:18:14,720 +defective بالمقارنة مع الصحيح 100 لـ 1، تخيلوا واحد + +248 +00:18:14,720 --> 00:18:20,200 +بس الصحيح و100، قد يصل ممكن 50 50 ممكن 70 30 لكن + +249 +00:18:20,200 --> 00:18:24,000 +في الآخر في نسبة معينة.. نسبة معينة من الـ + +250 +00:18:24,000 --> 00:18:27,240 +defective virus معروف أنها بتنتج في كل cycle + +251 +00:18:27,240 --> 00:18:32,400 +الفيروس طب نتخيل مع بعض دخل 100 فيروس عمل + +252 +00:18:32,400 --> 00:18:37,370 +infection لـ 100 خلية مليار مليار مليار مليار مليار + +253 +00:18:37,370 --> 00:18:38,210 +مليار مليار مليار مليار مليار مليار مليار مليار + +254 +00:18:38,210 --> 00:18:52,930 +مليار مليار مليار + +255 +00:19:04,590 --> 00:19:08,010 +الجهاز المناعي، إذا الـ defective virus استخدم في + +256 +00:19:08,010 --> 00:19:11,410 +تفسير لماذا هناك شفاء تلقائي لبعض الأمراض + +257 +00:19:11,410 --> 00:19:16,150 +الفيروسية نجد الثاني اللي هو الـ pseudo virus، + +258 +00:19:16,150 --> 00:19:22,910 +pseudo معناه كاذب هو شكله virus لكن من جوه جبله مش + +259 +00:19:22,910 --> 00:19:28,520 +virus، جبله host ماخد DNA من اللي هو الـ cell اللي + +260 +00:19:28,520 --> 00:19:31,340 +شفناها لما درسنا في الـ bacteriophage لما اتكلمنا + +261 +00:19:31,340 --> 00:19:34,520 +عن الـ generalized transduction و أن خلال التعبئة + +262 +00:19:34,520 --> 00:19:39,700 +قد يدخل إلى داخله بكتيريا دي إن أي صح؟ وبعدين ينتقل + +263 +00:19:39,700 --> 00:19:42,560 +هذا بيسميه pseudo variant وهذا okay بيعمل + +264 +00:19:42,560 --> 00:19:46,340 +infection لكن لن يحدث تكاثر، مافيش تكاثر، بيصيب + +265 +00:19:46,340 --> 00:19:50,320 +خلية ويدخل داخلها لكن مافيش تكاثر يعني they can + +266 +00:19:50,320 --> 00:19:53,560 +infect cell but they do not replication تمام؟ + +267 +00:19:53,560 --> 00:19:54,060 +مافيش replication + +268 +00:20:03,050 --> 00:20:07,430 +هو .. هو عبارة عن .. شوفنا العجاب في الـ micro + +269 +00:20:07,430 --> 00:20:10,870 +biology كنا نتكلم عن بكتيريا وبعدها نتكلم عن + +270 +00:20:10,870 --> 00:20:14,110 +كلاميديا والكيتسيا اللي هو الـ obligated cell وبعد + +271 +00:20:14,110 --> 00:20:16,350 +بعدها انتقلنا للفيروسات وبيقولنا الفيروسات حاجة + +272 +00:20:16,350 --> 00:20:19,630 +غريبة وبعدها انتقلنا من ديوال الفيروسات إلى + +273 +00:20:19,630 --> 00:20:23,730 +الفيرويدس الفيرويدس عبارة عن مادة وراثية مكونة من + +274 +00:20:23,730 --> 00:20:29,590 +mRNA فقط مافيش لا بروتين ولا غيره ولا كبسيد ولا + +275 +00:20:29,590 --> 00:20:33,610 +ولا أي حاجة الميزة الوحيدة أن هذا الـ mRNA إذا دخل + +276 +00:20:33,610 --> 00:20:38,450 +خلية معينة من الـ host .. suitable host بيعمل + +277 +00:20:38,450 --> 00:20:41,810 +replication وهم يمكنوا يعملوا infection على الرغم + +278 +00:20:41,810 --> 00:20:46,890 +من أن العلماء لم يجدوا في الـ mRNA هذا أي code لأي + +279 +00:20:46,890 --> 00:20:52,870 +protein، لحد الآن مافيش شفرة لبناء المادة + +280 +00:20:52,870 --> 00:20:58,210 +الوراثية لأنه فيها homology كتير باللاجئ طبعًا هي + +281 +00:20:58,210 --> 00:21:01,170 +circle لو أخذوا بالكم لكن لأنه فيها homology كتير + +282 +00:21:01,170 --> 00:21:05,190 +باللاجئ فيها صار loops إيش يعني loops؟ يعني + +283 +00:21:05,190 --> 00:21:09,130 +اتشابكوا مع بعض لإن هو هذا مرتبط مع هذا فصار عندي + +284 +00:21:09,130 --> 00:21:14,810 +loops يصيب النباتات ويسبب بعض الأمراض للنباتات لكن + +285 +00:21:14,810 --> 00:21:19,150 +لم يثبت أنه الـ viroids بتسبب للإنسان أو الحيوان أي + +286 +00:21:19,150 --> 00:21:27,170 +من العُضّار defective, pseudo و viroid الرابع الأهم + +287 +00:21:27,170 --> 00:21:30,310 +على الإطلاق وجيد من أنا أبدأ فيه أبدأ أدخل فيه + +288 +00:21:30,310 --> 00:21:38,110 +أعمل عرض برضه صغير أوضح إيش يعني شخص بيتناول غذاء + +289 +00:21:38,110 --> 00:21:42,170 +معين محتوى على الـ بريون الـ بريون عبارة عن مختصر + +290 +00:21:42,170 --> 00:21:45,810 +لكلمة protein-infectious هذا الغريب في الموضوع + +291 +00:21:45,810 --> 00:21:50,390 +protein-infectious بريون صار لا يحتوي على مادة + +292 +00:21:50,390 --> 00:21:54,870 +وراثية ولا بيحتوي على كبسيد ولا أي .. فقط بروتين + +293 +00:21:54,870 --> 00:21:59,310 +من نوع معين وهنتفاجأ أن هذا البروتين هو البروتين + +294 +00:21:59,310 --> 00:22:05,210 +إحنا بنتجه .. إحنا بنتجه بشكل طبيعي لكن انتجه + +295 +00:22:05,210 --> 00:22:09,810 +بشكل X يتحول لـ Y تختلف تمامًا بيصير abnormal + +296 +00:22:09,810 --> 00:22:12,750 +والمشكلة زمان إحنا كنا نقول أن اللي بيمشي مع + +297 +00:22:12,750 --> 00:22:15,770 +الفاسد إيش بيصير معها بيصير معها الفاسد وهو هذا + +298 +00:22:15,770 --> 00:22:19,710 +حقيقة صح ولا الفاسد بيحاول يفسد الناس اللي حواليه + +299 +00:22:19,710 --> 00:22:24,770 +وهذا بيشتغل بنفس الطريقة فساد هو تغير من الف إلى ر + +300 +00:22:24,770 --> 00:22:30,710 +من alpha confirmation إلى beta shaded أو beta + +301 +00:22:30,710 --> 00:22:35,890 +bladed shaded يتحول فكل ما بيمس normal بيحول لـ أوضه + +302 +00:22:35,890 --> 00:22:39,730 +normal فالتكاثر من وين جاي من بره أو تكاثر بالـ + +303 +00:22:39,730 --> 00:22:42,170 +simple binary vision أو بإنه بيدخل دلوقتي الخلايا + +304 +00:22:42,170 --> 00:22:47,850 +وبيتكاثر لأ فقط بيصير وكأنه جالب إذا مسك الـ + +305 +00:22:47,850 --> 00:22:52,330 +remote control هذا بيجلبه تهمهم صار من النوع + +306 +00:22:52,330 --> 00:22:58,670 +الخبيث لذلك الاسم بتعرفش عليه أساسًا لأن هو أصلاً + +307 +00:22:58,670 --> 00:23:02,790 +ابنه، من الجسم، من الجسم فبالتالي بيخرب الدماغ، + +308 +00:23:02,790 --> 00:23:07,270 +بيصير فيه زي الإسفنجة، مخزّج، تمام؟ ثقوب، أي شيء + +309 +00:23:07,270 --> 00:23:12,110 +بيسموه spongiform encephalopathy مرض في الدماغ + +310 +00:23:12,110 --> 00:23:15,970 +شكله .. شكله إسفنجي لأن الدماغ بعد تشريحه بيكون + +311 +00:23:15,970 --> 00:23:24,010 +بهذا الشكل خلينا نطلع عليه هي + +312 +00:23:24,010 --> 00:23:29,940 +الـ normal bryons اللي موجودة هي الـ abnormal، الآن + +313 +00:23:29,940 --> 00:23:32,520 +الـ abnormal هي من الاسم غير من الخارج إحنا، مع + +314 +00:23:32,520 --> 00:23:34,720 +التعقيدات اللي بيسموها transmissible، إيش هي + +315 +00:23:34,720 --> 00:23:39,360 +transmissible؟ قابل للانتقال، شوفوا الآن، مجرد أنه + +316 +00:23:39,360 --> 00:23:43,200 +اتلامس مع الـ normal صار abnormal، يبقى التكاثر تبعه + +317 +00:23:43,200 --> 00:23:47,580 +معتمد على الموجوده، هحكي بعض الحقائق تثبت الكلام هذا + +318 +00:23:51,980 --> 00:23:55,340 +طبعًا حد هيقولي إيش الـ bryons؟ الـ bryons عبارة عن + +319 +00:23:55,340 --> 00:23:58,760 +receptors موجودة في الخلايا تبعتنا لكن الوظيفة + +320 +00:23:58,760 --> 00:24:05,040 +تبعتهم لحد الآن علامة استفهام ليش علامة استفهام؟ + +321 +00:24:05,040 --> 00:24:08,140 +البعض طبعًا بيقول إن هي receptor إيه اللي علاقة بالـ + +322 +00:24:08,140 --> 00:24:11,480 +transduction عارفين شو هي transduction؟ إيش هي + +323 +00:24:11,480 --> 00:24:13,620 +transduction؟ + +324 +00:24:27,430 --> 00:24:32,270 +أي خلية نفترض إنّها خاملة في عليها receptor عليها + +325 +00:24:32,270 --> 00:24:37,070 +receptors كتيرة وأنا أرجو إنك تتخيل الخلية عبارة عن + +326 +00:24:37,070 --> 00:24:42,470 +كائن فضائي .. كائن فضائي مليء بالشعر الدقيق الطويل + +327 +00:24:42,470 --> 00:24:46,470 +الجزئ الأحمر الأصفر الأحمر اللوي غنية جدًا بالـ + +328 +00:24:46,470 --> 00:24:51,270 +receptors ما ننظر إليها إنها عبارة عن صندوق أملس + +329 +00:24:51,270 --> 00:24:56,650 +لأ الخلية أعقد من ذلك بكثير فيها Receptor أو + +330 +00:24:56,650 --> 00:25:01,190 +Receptors عفوًا أيّ Molecule ويرتبط بالـ Receptor + +331 +00:25:01,190 --> 00:25:07,010 +مجرد أن الـ Molecule يرتبط بالـ Receptor الـ Receptor + +332 +00:25:07,010 --> 00:25:09,550 +بيصير له activation بيعمل activation لمركبات جوه + +333 +00:25:09,550 --> 00:25:12,950 +المركبات اللي جوه بتنقل إشارة إشارة إشارة هذا اللي + +334 +00:25:12,950 --> 00:25:16,170 +عايز نسميه إحنا إيه Signal Signal إشارة Signal + +335 +00:25:16,170 --> 00:25:20,790 +Transduction فيعتقد إن من الـ Molecules المهمة في + +336 +00:25:20,790 --> 00:25:23,510 +مين في الـ Signal Transduction لكن + +337 +00:25:26,550 --> 00:25:30,810 +لكن بنطلق مصطلح اليوم جديد عليكم اسمه knockout + +338 +00:25:30,810 --> 00:25:36,050 +mice إيش يعني knockout؟ knockout mice هي فئران + +339 +00:25:36,050 --> 00:25:41,710 +تنتج بحيث إنه يكون فيها خلل في جين محدد فلما أقول + +340 +00:25:41,710 --> 00:25:49,130 +Bryans knockout mice يعني فئران فيها خلل في الجين + +341 +00:25:49,130 --> 00:25:54,250 +تبع مين؟ تبع الـ Bryans لما طعمناها .. أعطيناها يعني + +342 +00:25:54,250 --> 00:25:58,290 +.. أو حقنناها بالـ up normal brain ما صار عندها + +343 +00:25:58,290 --> 00:26:02,050 +المرض لأن + +344 +00:26:02,050 --> 00:26:04,850 +مافيش فيها الـ gene وبالتالي مش هيتكاثر أثر لأن + +345 +00:26:04,850 --> 00:26:08,690 +مافيش فيها الـ protein صح الـ normal protein المشكلة + +346 +00:26:08,690 --> 00:26:14,270 +أن الفئران اللي مافيش فيها الـ brains طبعًا برضه عاشت + +347 +00:26:14,270 --> 00:26:18,760 +normal بمعنى إنه ما تأثرتش الوظيفة تبعته لذلك نقول + +348 +00:26:18,760 --> 00:26:23,140 +أن الوظيفة تبعت Libraions is still unknown أو + +349 +00:26:23,140 --> 00:26:29,340 +unclear لكن الدراسات أثبتت إنه مستوى Libraion + +350 +00:26:29,340 --> 00:26:34,680 +كقياس في الـ infected والـ non-infected هو واحد والدراسات + +351 +00:26:34,680 --> 00:26:38,760 +أثبتت أن الـ mRNA تبع الجين تبع Libraions + +352 +00:26:38,760 --> 00:26:42,440 +في الـ infected والـ non-infected برضه واحد فا إيش + +353 +00:26:42,440 --> 00:26:47,390 +الضرر نفسر هنا التفسير كان التالي إن التغيير هو post + +354 +00:26:47,390 --> 00:26:51,970 +-translational إيش يعني post-translational يعني + +355 +00:26:51,970 --> 00:26:55,830 +بعد الـ protein level اللي هو اتبعتنا اللي قولنا + +356 +00:26:55,830 --> 00:27:01,610 +Alpha تحول إلى Beta بليتشيت وبالتالي بالفعل صار هو + +357 +00:27:01,610 --> 00:27:06,370 +أكبر إذا في مجموعة من هذه الحقائق قالت لنا إن الـ + +358 +00:27:06,370 --> 00:27:10,690 +bryons عبارة عن بروتينات normal موجودة في خليانا + +359 +00:27:10,900 --> 00:27:14,180 +لكن الـ abnormal form هو اللي بيخليها abnormal + +360 +00:27:14,180 --> 00:27:20,580 +وكونها self فالبسم بيخرب بيتدمر الدماغ دون أن يكون + +361 +00:27:20,580 --> 00:27:23,960 +هناك immune response دون أن يكون هناك inflammatory + +362 +00:27:23,960 --> 00:27:27,800 +response يعني لا فيه inflammation لا فيه اللي هو + +363 +00:27:27,800 --> 00:27:32,140 +ال .. ال .. ال .. إحدى النقاط المهمة اللي بتتكلم + +364 +00:27:32,140 --> 00:27:36,700 +فيها أن الـ bryons are more resistant than viruses + +365 +00:27:36,700 --> 00:27:39,880 +to inactivation by heat or by ultraviolet + +366 +00:27:39,880 --> 00:27:44,340 +radiation تخيلين؟ يعني الآن صارت عملية تعقيم + +367 +00:27:44,340 --> 00:27:49,460 +والتطهير أصعب من دي قبل لإن الـ UV كانت ممتازة في + +368 +00:27:49,460 --> 00:27:53,340 +قتل الفيروسات والبكتيريا لكن الآن هي مش ممتازة في + +369 +00:27:53,340 --> 00:27:57,920 +قتل الـ primers حتى الـ formaldehyde اللي لما + +370 +00:27:57,920 --> 00:28:01,260 +اتكلمنا عنه الـ antiseptic disinfectant كنا عنه من + +371 +00:28:01,260 --> 00:28:02,420 +أعظم ومن أقوى + +372 +00:28:05,730 --> 00:28:09,370 +لكن هناك مركبات هو الحقيقة sensitive لها زي مثلًا + +373 +00:28:09,370 --> 00:28:13,850 +اللي هو الـ hypochlorite والهيبكلوريت والانيواتش + +374 +00:28:13,850 --> 00:28:19,450 +والأوتوكليفن كفين لقتل الميكروب طالما المواد اللي + +375 +00:28:19,450 --> 00:28:21,610 +خايفين أنا أعمل أوتوكليفن ممكن أعاقّنها بمين + +376 +00:28:21,610 --> 00:28:27,060 +بالـhypochlorite اه بدي بس أذكر بعض الأمثلة + +377 +00:28:27,060 --> 00:28:30,580 +بشكل عام سمّنها transmissible spongiform + +378 +00:28:30,580 --> 00:28:33,900 +encephalopathy كلمة transmissible يعني قبل أن + +379 +00:28:33,900 --> 00:28:37,260 +تتقال، spongiform قلنا شكله شكل الدماغ زي + +380 +00:28:37,260 --> 00:28:40,420 +الأسفنجة، الدماغ بيكون encephalopathy لأنه في + +381 +00:28:40,420 --> 00:28:47,460 +الدماغ أشهر مثلين واحد بشري اسمه Creutzfeldt-Jakob + +382 +00:28:47,460 --> 00:28:53,530 +Disease Kreutzfeldt-Jakob Disease وهو يماثل مرض + +383 +00:28:53,530 --> 00:28:57,190 +جنون البقر، مرض جنون البقر، يعني هذا اسمه جنون + +384 +00:28:57,190 --> 00:29:00,730 +البشر، جنون البشر اسمه Jakob أو Kreutzfeldt-Jakob + +385 +00:29:00,730 --> 00:29:04,690 +Disease وفي مرض ثاني في الخراف بيسموه Scrabie، وهو + +386 +00:29:04,690 --> 00:29:10,850 +الأول، والأشهر على الإطلاق Scrabie، وبالتالي حتى + +387 +00:29:10,850 --> 00:29:15,170 +المسماء تبع ال .. ال .. ال .. بيسموها Prions P-R-I-O-N-S + +388 +00:29:15,170 --> 00:29:18,010 +-S-C، S-C من Scrabie، S-C من Scrabie + +389 +00:29:21,680 --> 00:29:25,480 +النقاط المهمة كمان كونه لا يحتوي على مادة وراثية + +390 +00:29:25,480 --> 00:29:28,080 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +391 +00:29:28,080 --> 00:29:28,700 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +392 +00:29:28,700 --> 00:29:30,400 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +393 +00:29:30,400 --> 00:29:31,880 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +394 +00:29:31,880 --> 00:29:33,580 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +395 +00:29:33,580 --> 00:29:34,740 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +396 +00:29:34,740 --> 00:29:34,940 +ال ال ال ال ال ال ال ال ال ال ال + +397 +00:29:42,670 --> 00:29:46,110 +الـ appearance in the electron microscope الـ + +398 +00:29:46,110 --> 00:29:49,910 +prions appear filamentous بينما الفيروس اما تكون + +399 +00:29:49,910 --> 00:29:53,050 +إيكوزاهيدرال أو الهيليكل اللي حكينا عنها أو ال + +400 +00:29:53,050 --> 00:29:56,870 +complex confirmation الجنود الأخيرة اتفقنا عليها + +401 +00:29:56,870 --> 00:30:01,710 +أنه no immunity is induced و no inflammation there + +402 +00:30:01,710 --> 00:30:06,410 +الحالة + +403 +00:30:06,410 --> 00:30:12,770 +ذراعها من 27 ل 30 كيلو دالتون بيسموه كيلو دالتون، + +404 +00:30:12,770 --> 00:30:16,130 +هذا وزن، وزن ال .. ال .. ال protein نفسه، nuclear + +405 +00:30:16,130 --> 00:30:19,910 +weight وهي أبدا ال glycoprotein زي ما حكينا بتكون + +406 +00:30:19,910 --> 00:30:25,090 +encoded by our own genes، في أن الكلام اللي حكيناه + +407 +00:30:25,090 --> 00:30:27,970 +قبل شوية المتعلق والباص transitional .. + +408 +00:30:27,970 --> 00:30:31,430 +translational modification، أنه تحويل بتنبع ال + +409 +00:30:31,430 --> 00:30:37,060 +translationهي Alpha و هي Beta تتغير تمام؟ واللي + +410 +00:30:37,060 --> 00:30:39,700 +اتشرحه اللي شرحته أنا على ال knockout mice اللي هو + +411 +00:30:39,700 --> 00:30:44,540 +أن ال knockout mice ماتأثرتش لما حفنها بال scrubby + +412 +00:30:44,540 --> 00:30:49,640 +برايونز لأنه مافيش فيها normal protein + +413 +00:30:51,100 --> 00:30:54,020 +زي ما حكيتها ال function كمان مش واضحة فيها اعتقاد + +414 +00:30:54,020 --> 00:30:57,740 +أن هي علاقة بال signal transduction لكن كما + +415 +00:30:57,740 --> 00:31:02,340 +ذكرتلكم لأ لا يوجد هناك إثبات حقيقي لهذا الدور لأن + +416 +00:31:02,340 --> 00:31:07,060 +ال mice اللي مافيهاش PRP normal برضه ما اتأثرت + +417 +00:31:07,060 --> 00:31:11,360 +بهذا البرنامج في بعض الروابط والفيديوهات اللي في + +418 +00:31:11,360 --> 00:31:15,100 +نهاية المحاضرة ممكن تشوفوهم على اليوتيوب ونكمل إن + +419 +00:31:15,100 --> 00:31:16,160 +شاء الله يوم الأربعاء diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/OeCcJ-QHmoY_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/OeCcJ-QHmoY_raw.json new file mode 100644 index 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{"start": 819.11, "end": 819.21, "word": " ال", "probability": 0.82568359375}, {"start": 819.21, "end": 819.63, "word": " membrane", "probability": 0.93212890625}, {"start": 819.63, "end": 819.95, "word": " زي", "probability": 0.813720703125}, {"start": 819.95, "end": 820.03, "word": " ما", "probability": 0.70068359375}, {"start": 820.03, "end": 820.17, "word": " انتوا", "probability": 0.7007649739583334}, {"start": 820.17, "end": 820.37, "word": " زي", "probability": 0.5355224609375}, {"start": 820.37, "end": 820.51, "word": " ما", "probability": 0.826171875}, {"start": 820.51, "end": 820.73, "word": " انا", "probability": 0.6904296875}, {"start": 820.73, "end": 820.95, "word": " بدي", "probability": 0.6685791015625}, {"start": 820.95, "end": 821.39, "word": " اتحرك", "probability": 0.9473876953125}], "temperature": 1.0}, {"id": 33, "seek": 84451, "start": 822.35, "end": 844.51, "text": "و بياخد جزء و بيسموه العملية body كأنه تبرع و كأنه أخد قطعة و طلع فيها فلكن ماصارش ايه؟ destruction ل الخلايا هاي ال glycoprotein زي ما حكينا و بترسلوها ال GP", "tokens": [2407, 4724, 1829, 47283, 3215, 10874, 11622, 38207, 4032, 4724, 1829, 38251, 2407, 3224, 18863, 42213, 10632, 1772, 9122, 33456, 3224, 6055, 26890, 3615, 4032, 9122, 33456, 3224, 5551, 9778, 3215, 12174, 9566, 27884, 4032, 23032, 1211, 3615, 8978, 11296, 6156, 1211, 19452, 19446, 9381, 9640, 8592, 1975, 1829, 3224, 22807, 13563, 5296, 33962, 15040, 25528, 8032, 47302, 2423, 22633, 13084, 81, 1370, 259, 30767, 1829, 19446, 11331, 4117, 1829, 8315, 4032, 39894, 2288, 3794, 1211, 2407, 11296, 2423, 26039], "avg_logprob": -0.3013117225081832, "compression_ratio": 1.4065934065934067, "no_speech_prob": 0.0, "words": [{"start": 822.35, "end": 822.63, "word": "و", "probability": 0.6923828125}, {"start": 822.63, "end": 823.71, "word": " بياخد", "probability": 0.72686767578125}, {"start": 823.71, "end": 824.59, "word": " جزء", "probability": 0.9768880208333334}, {"start": 824.59, "end": 824.69, "word": " و", 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الفقران", "probability": 0.8956298828125}, {"start": 1570.13, "end": 1570.27, "word": " اللي", "probability": 0.950439453125}, {"start": 1570.27, "end": 1570.55, "word": " مافيش", "probability": 0.9254557291666666}, {"start": 1570.55, "end": 1571.05, "word": " فيها", "probability": 0.963623046875}, {"start": 1571.05, "end": 1571.53, "word": " ال", "probability": 0.2454833984375}, {"start": 1571.53, "end": 1571.97, "word": " brains", "probability": 0.568359375}, {"start": 1571.97, "end": 1572.59, "word": " طبعا", "probability": 0.76617431640625}, {"start": 1572.59, "end": 1573.97, "word": " برضه", "probability": 0.8444010416666666}, {"start": 1573.97, "end": 1574.27, "word": " عشت", "probability": 0.8347981770833334}, {"start": 1574.27, "end": 1574.59, "word": " normal", "probability": 0.80712890625}], "temperature": 1.0}, {"id": 62, "seek": 160326, "start": 1575.9, "end": 1603.26, "text": "بمعنى انه ماتأثرتش الوظيفة تبعته لذلك بنقول ان الوظيفة تبعت Libraions is still unknown او unclear لكن الدراسات أثبتت انه مستوى Libraion كقياس في ال infected و ال non-infected هو واحد و الدراسات أثبتت ان ال mRNA تبع الجين تبع Libraions في ال infected و ال non-infected برضه واحد فايش ضرر نفسر هنا", "tokens": [3555, 2304, 3615, 1863, 7578, 16472, 3224, 3714, 9307, 10721, 49115, 2655, 8592, 2423, 2407, 19913, 33911, 3660, 6055, 3555, 34268, 3224, 5296, 8848, 23275, 44945, 39648, 16472, 2423, 2407, 19913, 33911, 3660, 6055, 3555, 34268, 15834, 424, 626, 307, 920, 9841, 1975, 2407, 25636, 44381, 32748, 23557, 3794, 9307, 5551, 12984, 3555, 2655, 2655, 16472, 3224, 3714, 14851, 2407, 7578, 15834, 424, 313, 9122, 4587, 25528, 3794, 8978, 2423, 15414, 4032, 2423, 2107, 12, 26260, 292, 31439, 36764, 24401, 4032, 32748, 23557, 3794, 9307, 5551, 12984, 3555, 2655, 2655, 16472, 2423, 50103, 6055, 3555, 3615, 25724, 9957, 6055, 3555, 3615, 15834, 424, 626, 8978, 2423, 15414, 4032, 2423, 2107, 12, 26260, 292, 4724, 43042, 3224, 36764, 24401, 6156, 47302, 8592, 48812, 2288, 2288, 8717, 36178, 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1599.68, "end": 1599.78, "word": " ال", "probability": 0.880859375}, {"start": 1599.78, "end": 1599.96, "word": " non", "probability": 0.96875}, {"start": 1599.96, "end": 1600.54, "word": "-infected", "probability": 0.9181315104166666}, {"start": 1600.54, "end": 1601.12, "word": " برضه", "probability": 0.9560546875}, {"start": 1601.12, "end": 1601.5, "word": " واحد", "probability": 0.990234375}, {"start": 1601.5, "end": 1602.44, "word": " فايش", "probability": 0.623779296875}, {"start": 1602.44, "end": 1602.62, "word": " ضرر", "probability": 0.76953125}, {"start": 1602.62, "end": 1603.06, "word": " نفسر", "probability": 0.67431640625}, {"start": 1603.06, "end": 1603.26, "word": " هنا", "probability": 0.865234375}], "temperature": 1.0}, {"id": 63, "seek": 163069, "start": 1604.15, "end": 1630.69, "text": "التفسير كان تالي إن التغيير هو post-translational إيش يعني post-translational يعني بعد ال protein level اللي هو اتبعتنا اللي قولنا Alpha تحول إلى Beta بليتشيت وبالتالي بالفعل صار هو أكبر إذا في مجموعة من هذه الحقائق جلتلنا إن ال bryons عبارة عن بروتينات normal موجودة في خليانة", "tokens": [6027, 2655, 36178, 13546, 25961, 6055, 6027, 1829, 36145, 16712, 17082, 1829, 13546, 31439, 2183, 12, 24999, 75, 1478, 11933, 1829, 8592, 37495, 22653, 2183, 12, 24999, 75, 1478, 37495, 22653, 39182, 2423, 7944, 1496, 13672, 1829, 31439, 1975, 2655, 3555, 34268, 8315, 13672, 1829, 12174, 12610, 8315, 20588, 6055, 5016, 12610, 30731, 33286, 4724, 1211, 36081, 8592, 36081, 46599, 6027, 2655, 6027, 1829, 20666, 5172, 30241, 20328, 9640, 31439, 5551, 4117, 26890, 11933, 15730, 8978, 3714, 7435, 2304, 2407, 27884, 9154, 29538, 21542, 4587, 16373, 4587, 10874, 1211, 2655, 1211, 8315, 36145, 2423, 272, 627, 892, 6225, 3555, 9640, 3660, 18871, 4724, 32887, 31371, 8315, 2655, 2710, 3714, 29245, 23328, 3660, 8978, 16490, 20292, 7649, 3660], "avg_logprob": -0.2633739406779661, "compression_ratio": 1.6441947565543071, "no_speech_prob": 0.0, "words": [{"start": 1604.15, "end": 1604.79, 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1630.17, "end": 1630.69, "word": " خليانة", "probability": 0.77392578125}], "temperature": 1.0}, {"id": 64, "seek": 166028, "start": 1630.9, "end": 1660.28, "text": "لكن الـ abnormal form هو اللي بيخليها abnormal وكونها self فالبسم بيخرب بيتدمر الدماغ دون أن يكون هناك immune response دون أن يكون هناك inflammatory response يعني لا فيه inflammation لا فيه اللي هو ال .. ال .. ال .. أحد النقاط المهمة اللي بتتكلم فيها أن ال bryons are more resistant than viruses to inactivation by heat or by ultraviolet radiation", "tokens": [1211, 19452, 2423, 39184, 32847, 1254, 31439, 13672, 1829, 4724, 1829, 9778, 20292, 11296, 32847, 4032, 30544, 11296, 2698, 6156, 6027, 3555, 38251, 4724, 1829, 9778, 25513, 4724, 36081, 3215, 29973, 32748, 2304, 5718, 118, 11778, 11536, 14739, 7251, 30544, 34105, 4117, 11992, 4134, 11778, 11536, 14739, 7251, 30544, 34105, 4117, 38199, 4134, 37495, 22653, 20193, 8978, 3224, 21613, 20193, 8978, 3224, 13672, 1829, 31439, 2423, 4386, 2423, 4386, 2423, 4386, 5551, 24401, 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أنهيناه اللي هو الـ bacterial cell structure + +4 +00:00:35,420 --> 00:00:38,040 +وبالتالي من المناسب أن نبدأ إن شاء الله بالمخاطرة + +5 +00:00:38,040 --> 00:00:40,380 +بأنواع الـ bacterial physiology + +6 +00:00:43,590 --> 00:00:47,450 +كأي خلية في الدنيا هناك مكونات لهذه الخلية غالباً + +7 +00:00:47,450 --> 00:00:51,510 +نستطيع أن نصنفها بتصنيفات مختلفة مثلاً كـ chemical + +8 +00:00:51,510 --> 00:00:55,570 +component فيها الـ water لكن الـ water بدنا نغير + +9 +00:00:55,570 --> 00:00:58,790 +مفهومنا عنه اليوم نتكلم عنه علم الـ water free + +10 +00:00:58,790 --> 00:01:04,690 +water و bound water يعني قد نرى بعض الأطعمة على + +11 +00:01:04,690 --> 00:01:08,190 +سبيل المثال المربى المربى يحتوي كمية كبيرة من + +12 +00:01:08,190 --> 00:01:12,760 +الماء Jelly-like يعني واضح أنه من القوام تبعه أنه + +13 +00:01:12,760 --> 00:01:16,740 +سيحتفظ بكمية كبيرة من الماء لكن لو وضعنا + +14 +00:01:16,740 --> 00:01:21,160 +بكتيريا في الـ jelly هنجد أن البكتيريا غير قادرة + +15 +00:01:21,160 --> 00:01:26,180 +على النمو بالعكس الماء اللي جواها تفقد لصالح من؟ + +16 +00:01:26,180 --> 00:01:30,020 +المربى لأنه تركيز السكر عالي جداً فيه فهذا يعتبر + +17 +00:01:30,020 --> 00:01:35,530 +bound water يعني ماء مرتبط غير متاح للبكتيريا الماء + +18 +00:01:35,530 --> 00:01:38,530 +المتاح اللي هو الـ available water أو الـ free water + +19 +00:01:38,530 --> 00:01:42,730 +ويعبر عنه بما يسمى الـ water activity water + +20 +00:01:42,730 --> 00:01:47,990 +activity شايفين الرمز هذا الـ AW هذا بيعبر عن ما + +21 +00:01:47,990 --> 00:01:52,490 +يسمى بالـ water activity inorganic salts أملاح غير + +22 +00:01:52,490 --> 00:01:55,410 +عضوية زي الفوسفور والمغنيسيوم والكالسيوم + +23 +00:01:55,410 --> 00:02:01,450 +والصوديوم لبروتينات وتشكل كمية كبيرة من الوزن + +24 +00:02:01,450 --> 00:02:05,490 +الجاف للخلية حوالي خمسين لثمانين بالمئة من وزن + +25 +00:02:05,490 --> 00:02:08,790 +الخلية الجاف وعبارة عن بروتينات وأنا بتصوروا + +26 +00:02:08,790 --> 00:02:12,620 +البروتينات مما تتكون تتكون البروتينات من كربون ومن + +27 +00:02:12,620 --> 00:02:15,840 +نيتروجين ومن سلفر ومن other components يعني + +28 +00:02:15,840 --> 00:02:21,380 +البروتين كمركب يشتمل على مركبات أخرى الـ sugar أو + +29 +00:02:21,380 --> 00:02:28,580 +السكريات وأذكركم فقط بقضية الـ .. الـ .. الـ .. الـ + +30 +00:02:28,580 --> 00:02:32,120 +بتيدوجلايكان الـ بتيدوجلايكان أصلاً مكون من + +31 +00:02:32,120 --> 00:02:36,020 +N-acetylglucosamine و N-acetylmuramic acid والتنين + +32 +00:02:36,020 --> 00:02:41,180 +عبارة عن carbohydrates أو sugars أيضاً الـ capsule + +33 +00:02:41,180 --> 00:02:44,560 +اللي بتتذكره المكونة كانت من polysaccharide كان + +34 +00:02:44,560 --> 00:02:49,380 +فينا الـ lipids أو الدهون فاكرين الـ phospholipids + +35 +00:02:49,380 --> 00:02:54,620 +الـ cell membrane وكان في الـ outer membrane تبع الـ + +36 +00:02:54,620 --> 00:02:57,360 +gram negative بكتيريا برضه كان فينا outer membrane + +37 +00:02:57,360 --> 00:03:02,000 +ومكون من اللي هو الـ lipids طبعاً في بعض البكتيريا + +38 +00:03:02,000 --> 00:03:05,980 +فيها fats أخرى بتكون فيها waxy lipids + +39 +00:03:09,380 --> 00:03:12,860 +دهون شمعية، وتذكر هذه الجملة كويس لأن هنستفيد + +40 +00:03:12,860 --> 00:03:16,140 +منها لما نتكلم عن الـ acid fast stain، هناك بعض + +41 +00:03:16,140 --> 00:03:18,880 +أنواع من البكتيريا غير قابلة للصبغة بالـ gram + +42 +00:03:18,880 --> 00:03:23,340 +stain وبالتالي بدنا نعرف السبب ليش غير قابلة + +43 +00:03:23,340 --> 00:03:28,360 +للصبغة نظراً لوجود طبقة من الـ waxy lipids في هذه + +44 +00:03:28,360 --> 00:03:31,500 +الخلايا طبعاً في أيضاً nucleic acids والـ + +45 +00:03:31,500 --> 00:03:36,080 +nucleic acid هي عبارة عن RNA and DNA بعكس + +46 +00:03:36,080 --> 00:03:39,720 +الفيروسات اللي ما تحدثنا كنا عن الفيروسات يا إما DNA + +47 +00:03:39,720 --> 00:03:41,320 +يا إما RNA + +48 +00:03:44,200 --> 00:03:49,160 +نظرة أخرى بطريقة أخرى على البكتيريا ياريتنا نركز + +49 +00:03:49,160 --> 00:03:53,380 +على اللوحة ونشوف أن اللوحة بتقولنا أن 50% من + +50 +00:03:53,380 --> 00:04:00,410 +وزن البكتيريا اللي جاي هو carbon 60% لـ 80% البروتين + +51 +00:04:00,410 --> 00:04:05,010 +موضوع كله البروتين تعبير آخر مختلف عن كلمة carbon + +52 +00:04:05,010 --> 00:04:08,510 +البروتين يحتوي على carbon وغير الـ carbon لكن هنا + +53 +00:04:08,510 --> 00:04:11,290 +نتكلم عن الـ carbon والـ carbon قد يكون موجود في الـ + +54 +00:04:11,290 --> 00:04:14,310 +carbohydrates وفي الـ lipids وفي الـ proteins في + +55 +00:04:14,310 --> 00:04:17,910 +عندي الـ oxygen يحتوي برضه أو نسبة كبيرة من الخلية + +56 +00:04:17,910 --> 00:04:21,750 +هي عبارة عن oxygen في عندي اللي هو الـ nitrogen + +57 +00:04:21,750 --> 00:04:26,950 +كمان بشكل 14% من الخلية في حين دي الهيدروجين ثمانية + +58 +00:04:26,950 --> 00:04:30,110 +في المئة، في حين دي الفوسفور، وفي حين السلفر + +59 +00:04:30,110 --> 00:04:34,630 +والبوتاسيوم والصوديوم بكميات أقل، إذا احنا متفقين + +60 +00:04:34,630 --> 00:04:40,050 +على أن تركيب الخلية تركيب الخلية قد ينظر إليه + +61 +00:04:40,050 --> 00:04:44,890 +بنظرات مختلفة وبأوجه مختلفة، وشوفنا أرقام اختلفت من + +62 +00:04:44,890 --> 00:04:49,420 +لوحة إلى لوحة ما أعرف اللي هو حين كان اللي أمامكم + +63 +00:04:49,420 --> 00:04:51,860 +الصغيرة اللي الخلوية الصغيرة أن هي أبقى عنصر و + +64 +00:04:51,860 --> 00:04:56,120 +احنا يعني فرضناها خالص فصعب الرؤية لكن هحاول أن + +65 +00:04:56,120 --> 00:05:01,100 +أقدر الإمكان أن أذكر أو مرور سريع على المركبات واقول لكم + +66 +00:05:01,100 --> 00:05:05,300 +الكم هذه المركبات وين موجودة فعشان بالمثال أن + +67 +00:05:05,300 --> 00:05:08,410 +الـ carbon أنا قلت الكربون مولود في وين؟ في + +68 +00:05:08,410 --> 00:05:10,990 +البروتين، مولود في الـ carbohydrates، مولود في + +69 +00:05:10,990 --> 00:05:14,950 +الـ lipids، موجود حتى في الـ nucleic acids وبالتالي + +70 +00:05:14,950 --> 00:05:18,490 +الكربون هو أكثر عنصر واحنا اعطيناها خمسين بالمئة + +71 +00:05:18,490 --> 00:05:23,810 +من وزن الخلية الجاف، هنا طبعاً أنا مهم جداً نعرف + +72 +00:05:23,810 --> 00:05:26,910 +الكربون وين موجود ومهم جداً نعرف وين النيتروجين + +73 +00:05:26,910 --> 00:05:30,250 +مولود، اللي عارفين ليش؟ لأن بكرة هنقول أن في حد + +74 +00:05:30,250 --> 00:05:35,960 +الـ media، وسط الزراعي، هذا الوسط الزراعي بدنا نوفر + +75 +00:05:35,960 --> 00:05:40,760 +للبكتيريا اللي بننميها مصادر كربونية مصادر + +76 +00:05:40,760 --> 00:05:43,840 +نيتروجينية بدنا نوفر لها minerals بدنا نوفر لها + +77 +00:05:43,840 --> 00:05:48,380 +water water activity كافية مناسبة فمعرفتي + +78 +00:05:48,380 --> 00:05:53,400 +بالمركبات المحتوية على تلك العناصر بدي أصنع الـ + +79 +00:05:53,400 --> 00:05:56,540 +media تبعتي بدون هذه المعرفة لن أستطيع أن أقوم + +80 +00:05:56,540 --> 00:06:00,920 +بتصنيع اللي هو الـ media فمهم جداً أن أعرف الكلام عند + +81 +00:06:00,920 --> 00:06:05,380 +الأكسجين والأهم مصدر له في الخلية هو من الـ water + +82 +00:06:05,380 --> 00:06:10,980 +الـ H2O كلكم بتعرفوا أن الوزن الرطب للخلية قد يشكل + +83 +00:06:10,980 --> 00:06:17,920 +70% الـ water من وزن الخلية الـ nitrogen والنيتروجين + +84 +00:06:17,920 --> 00:06:22,700 +أذكركم بمكونات بتعرفوها كويس وبتحتوي على نيتروجين + +85 +00:06:22,700 --> 00:06:27,250 +واحدة ماذا يسمى الـ .. الـ .. الـ .. الـ .. الـ + +86 +00:06:27,250 --> 00:06:30,870 +nitrogenous bases قواعد النيتروجينية اللي هم + +87 +00:06:30,870 --> 00:06:36,150 +الأدينين والجوانين والسيتوسين واليوراسيل + +88 +00:06:36,150 --> 00:06:41,310 +والثايمين نعم هدول كلهم قواعد نيتروجينية الـ amino + +89 +00:06:41,310 --> 00:06:44,930 +acids اسمه amino acid لإنه فيه amino group والـ + +90 +00:06:44,930 --> 00:06:49,010 +amino group هو الـ NH2 مظبوط الـ NH2 يبقى النيتروجين + +91 +00:06:49,010 --> 00:06:52,110 +موجود في الـ amino acids يعني في البروتينات إذا + +92 +00:06:52,110 --> 00:06:55,550 +قواعد نيتروجينية وبروتين و amino acids هذي + +93 +00:06:55,550 --> 00:06:59,790 +المصدر الرئيسي للنيتروجين فينا الـ .. الـ + +94 +00:06:59,790 --> 00:07:03,110 +hydrogen برضه المصدر الأساسي اللي هو الماء لأن هو + +95 +00:07:03,110 --> 00:07:08,720 +للأكسجين وللـ .. فينا الـ phosphorus فوسفور تذكروا + +96 +00:07:08,720 --> 00:07:12,920 +أن الـ backbone تبع المادة الوراثية DNA فيها + +97 +00:07:12,920 --> 00:07:17,340 +فوسفور في phosphate group في phosphate group تذكر + +98 +00:07:17,340 --> 00:07:20,940 +الـ phospholipids اسمها phospholipids يبقى فيها + +99 +00:07:20,940 --> 00:07:27,630 +أنها أيضاً phosphate group السلفر وأذكركم أن السلفر + +100 +00:07:27,630 --> 00:07:33,610 +اسمها sulfur containing amino acids يعني التصنيف + +101 +00:07:33,610 --> 00:07:37,290 +من تصنيفات الـ amino acids أن فيها كبريت خدوا بالكم + +102 +00:07:37,290 --> 00:07:42,710 +هذه الكميات والمصادر قد تختلف من microbe لـ microbe + +103 +00:07:42,710 --> 00:07:49,730 +بمعنى بكتيريا تعيش في قاع المحيطات لا تمتلك oxygen + +104 +00:07:49,730 --> 00:07:55,390 +molecular جزئي البتة لا تمتلك حتى إضاءة عارفين + +105 +00:07:55,390 --> 00:07:58,230 +العنصر الأساسي إيه لها التنفس هو الـ sulfur + +106 +00:07:58,230 --> 00:08:02,990 +الكبريت فهي بحاجة إلى كميات كبيرة من من الكبريت + +107 +00:08:02,990 --> 00:08:06,250 +بالنسبة للبكتيريا العادية الممرضة لأنها حالتها + +108 +00:08:06,250 --> 00:08:10,210 +الكبريت بتكون حاجة قليلة جداً حاجة قليلة جداً + +109 +00:08:11,160 --> 00:08:14,820 +البوتاسيوم والبوتاسيوم عبارة عن cofactor أيضاً كمان + +110 +00:08:14,820 --> 00:08:18,200 +المغنيسيوم والكالسيوم والحديد كلهم هدول نحتاجهم + +111 +00:08:18,200 --> 00:08:21,380 +بكميات قليلة وبيعملوا كـ cofactor وممكن يكونوا + +112 +00:08:21,380 --> 00:08:25,420 +موجودين في إنزيمات محددين وأشهر مثال الحديد + +113 +00:08:25,420 --> 00:08:29,060 +الحديد موجود في مجموعة من الإنزيمات اسمها + +114 +00:08:29,060 --> 00:08:35,420 +cytochromes والـ cytochromes هي مجموعة الإنزيمات + +115 +00:08:35,420 --> 00:08:42,280 +اللازمة لعملية الأكسدة في الخلية وإنتاج الطاقة اللوحة + +116 +00:08:42,280 --> 00:08:48,440 +اللي أمامنا الآن لوحة معبرة وفيها حجم معلومات ضخم + +117 +00:08:48,440 --> 00:08:56,780 +جداً يمكن التوقف عندها فترات طويلة لأن كل نقطة من هذه + +118 +00:08:56,780 --> 00:09:00,940 +النقاط يعني هي بحد ذاتها قد تكون فصل أو فصلين في + +119 +00:09:00,940 --> 00:09:04,620 +كتاب الـ nutrients الـ temperature الـ hydrogen ion + +120 +00:09:04,620 --> 00:09:08,020 +concentration أو الـ pH الـ oxygen requirement والـ + +121 +00:09:08,020 --> 00:09:14,530 +osmotic pressure نأخذ واحدة واحدة ونحاول أن بقدر + +122 +00:09:14,530 --> 00:09:17,330 +المقتصر المفيد لأن احنا قلنا essential + +123 +00:09:17,330 --> 00:09:21,790 +microbiology نحاول تقليل من حجم المعلومات لكن نعطي + +124 +00:09:21,790 --> 00:09:24,010 +معلومات مفيدة أننا في الـ diagnostic microbiome أن + +125 +00:09:24,010 --> 00:09:31,090 +شاء الله نأخذ أول معلومة لها الـ nutrients احنا + +126 +00:09:31,090 --> 00:09:38,240 +ممكن نصنف البكتيريا بحسب حاجتها للغذاء أو بطريقتها + +127 +00:09:38,240 --> 00:09:43,060 +في إنتاج أو استهلاك الغذاء إلى سنتين Autotroph ده + +128 +00:09:43,060 --> 00:09:47,880 +دي التغبية وهي يعني لن نتطرق لها في الـ medical + +129 +00:09:47,880 --> 00:09:51,440 +microbiology لأن كل ميكروباتنا احنا من نوع تاني + +130 +00:09:51,440 --> 00:09:55,440 +اللي هي الـ heterotroph و الـ heterotroph they + +131 +00:09:55,440 --> 00:10:02,140 +utilize ready made food يعني أغبية معدة سلفا معدة + +132 +00:10:02,140 --> 00:10:06,740 +وجاهزة من الآخرين الـ autotrophs تشبه النباتات + +133 +00:10:07,550 --> 00:10:12,070 +Photosynthetic بتصنع من الإضاءة و الـ CO2 + +134 +00:10:12,070 --> 00:10:17,190 +Carbohydrates تمام؟ فهي بيسموها Primary Producers، + +135 +00:10:17,190 --> 00:10:21,530 +منتجة، يعني مخلوقات منتجة، اللي أهميتها البيئية في + +136 +00:10:21,530 --> 00:10:24,970 +كوكب الأرض لكن من ناحية طبيعية ليس لنا بها أي + +137 +00:10:24,970 --> 00:10:29,850 +علاقة، حتى الـ Heterotroph بنصنفهم إلى صنفين + +138 +00:10:31,090 --> 00:10:37,070 +Saprophytes وهي باللغة العربية مترممة وكلمة مترممة + +139 +00:10:37,070 --> 00:10:42,170 +تطلق على الكائنات اللي تتغذى على مخلوقات ميتة على + +140 +00:10:42,170 --> 00:10:46,750 +مخلوقات ميتة النوع الثاني اللي هو الـ Parasitic أو + +141 +00:10:46,750 --> 00:10:49,950 +الـ Parasites وهي اللي بتطفل على المخلوقات الحية + +142 +00:10:49,950 --> 00:10:56,060 +اللي بتشمل الميكروبات اللي بتضرنا احنا كبشر إذا + +143 +00:10:56,060 --> 00:10:59,980 +كمان مرة Autotroph و Heterotroph و Heterotroph تسمى + +144 +00:10:59,980 --> 00:11:03,980 +اللي هم الـ Sabrophytes والثاني يقولنا الـ parasites + +145 +00:11:03,980 --> 00:11:07,340 +طيب + +146 +00:11:07,340 --> 00:11:10,920 +الـ nutrients الـ requirement بشكل عام للبكتيريا + +147 +00:11:10,920 --> 00:11:14,740 +تتلخص في مجموعة من العناصر الرئيسية الـ water + +148 +00:11:14,740 --> 00:11:21,460 +وتخيلوا إنه قد تحتاج إلى تسعين بالمية من المكونات + +149 +00:11:21,460 --> 00:11:26,370 +تكون water تفكرين كلمة water activity؟ كمان مرة + +150 +00:11:26,370 --> 00:11:31,650 +water activity بتعبر عن مين؟ عن الـ free water الـ + +151 +00:11:31,650 --> 00:11:35,290 +requirement للميكروبات تختلف وبالتالي عندما يطلب + +152 +00:11:35,290 --> 00:11:39,150 +مني أنا أن أتعامل مع ميكروب أنا ما بعرفه، وش دي؟ + +153 +00:11:39,150 --> 00:11:42,530 +جالي لبنان حاضر له وصد؟ وصد، من الأشياء اللي لازم + +154 +00:11:42,530 --> 00:11:45,810 +أدرسها عن هذا الميكروب هو الـ water activity + +155 +00:11:45,810 --> 00:11:50,130 +requirement كده بدي water activity؟ أو على الأقل + +156 +00:11:50,130 --> 00:11:55,050 +أعرف المعلومة هذه عشان أنا ممكن أفكر بطريقة ثانية، + +157 +00:11:55,050 --> 00:11:58,470 +أقول هذا الميكروب أنا بتعزله من بيئة، البيئة + +158 +00:11:58,470 --> 00:12:03,050 +بالمناسبة أنتم رايحين اليوم دخلت القاعة من المبنى + +159 +00:12:03,050 --> 00:12:07,320 +شميته رائحة المطر الجديدة؟ رياح دي المطر الجديدة دي + +160 +00:12:07,320 --> 00:12:11,640 +عبارة رائحة Actinomycetes بكتيريا طبعا بتشموها فقط + +161 +00:12:11,640 --> 00:12:15,920 +في .. في أول موسم هي من .. من الميكروبات الرائعة + +162 +00:12:15,920 --> 00:12:19,420 +اللي بتنتج لنا كمية كبيرة من الـ antibiotics وبتنتج + +163 +00:12:19,420 --> 00:12:22,300 +بعض الأحيان .. أو مش بعض الأحيان الـ soil + +164 +00:12:22,300 --> 00:12:27,440 +actinomycetes بتنتج اللي هي الـ aromatic substances + +165 +00:12:27,440 --> 00:12:33,080 +اللي احنا بنشتمها مع تفاعلها مع رذاذ المطر بس + +166 +00:12:33,080 --> 00:12:36,340 +حبينا نقول لكم إنه هذه الرائحة اللي .. اللي .. اللي + +167 +00:12:36,340 --> 00:12:39,460 +بتذكرنا ده أو .. أو ممكن نشتاق لها لأنها بتمر على + +168 +00:12:39,460 --> 00:12:43,940 +سنة حتى نشتمها مرة ثانية فهي رائحة لـ .. لـ .. + +169 +00:12:43,940 --> 00:12:48,120 +للنتيجة عن البكتيريا water, carbon source, nitrogen + +170 +00:12:48,120 --> 00:12:51,140 +source, minerals, growth factors و الـ growth + +171 +00:12:51,140 --> 00:12:55,900 +factors غالبا تطلق على عناصر البكتيريا غير قادرة + +172 +00:12:55,900 --> 00:12:59,180 +على أنها تصنعها وبالتالي بتحتاجها إن احنا نزود + +173 +00:12:59,180 --> 00:13:03,710 +الوسط فيهانرجح على الـ water water activity كمان + +174 +00:13:03,710 --> 00:13:07,390 +مرة قلنا لكم مطلوب نعرف عن الـ microbe الـ water + +175 +00:13:07,390 --> 00:13:13,950 +activity بدي أحصل microbe X من التربة وكلمة تربة + +176 +00:13:13,950 --> 00:13:17,690 +معناها حاجة إن في منافسين كثير مع هذا الـ microbe + +177 +00:13:19,420 --> 00:13:23,320 +فإذا علمت إن الـ requirement تبع هذا الـ microbe + +178 +00:13:23,320 --> 00:13:28,020 +unique، كلمة unique يعني فريدة، مميزة، أستطيع أن + +179 +00:13:28,020 --> 00:13:32,560 +أتحكم في الوسط، تمام؟، بحيث أن يلائم هذا الـ + +180 +00:13:32,560 --> 00:13:35,840 +microbe ولا يلائم ميكروبات أخرى، مصطلح بنسميه + +181 +00:13:35,840 --> 00:13:41,000 +selective isolation، عزل انتقائي أو عزل اختياري + +182 +00:13:42,770 --> 00:13:47,210 +selective isolation وفي المختبر بنعمل تقنية اسمها + +183 +00:13:47,210 --> 00:13:51,490 +selective media أو بوسطية انتقائية هنتكلم عنها في + +184 +00:13:51,490 --> 00:13:56,870 +وقت لاحق إن شاء الله طيب أنتم الآن لما أنا بقول + +185 +00:13:56,870 --> 00:14:06,070 +carbon source إيش بختاروا على بالكم؟ اللوحة + +186 +00:14:06,070 --> 00:14:11,880 +السابقة اللوحة السابقة إن الـ carbon موجود في الـ + +187 +00:14:11,880 --> 00:14:15,680 +carbohydrate في الـ proteins وبالتالي الآن .. الآن + +188 +00:14:15,680 --> 00:14:18,820 +إذا بدي carbon source بدي أعرف برضه حاجة البكتيريا + +189 +00:14:18,820 --> 00:14:24,360 +هل بيلزمها الجلوكوز فقط؟ هل بيلزمها starch؟ الـ + +190 +00:14:24,360 --> 00:14:28,000 +starch أرخص من الجلوكوز وبتنمو على الاثنين بحط لها + +191 +00:14:28,000 --> 00:14:33,520 +starch يبقى بدّا قلت لها carbon source وغالبا one + +192 +00:14:33,520 --> 00:14:38,610 +single carbon source ملائم يكفي البكتيريا تلميه one + +193 +00:14:38,610 --> 00:14:41,990 +single carbon source يعني بحط لها جلوكوز بحط لها + +194 +00:14:41,990 --> 00:14:45,810 +لاكتوز حسب نوعية البكتيريا الـ nitrogen source برضه + +195 +00:14:45,810 --> 00:14:50,330 +لازم مصادره الـ proteins digested amino acids أفضل + +196 +00:14:50,330 --> 00:14:53,670 +digested proteins مكسرة الـ amino acids نيتروجينس + +197 +00:14:53,670 --> 00:14:57,390 +بيز نيوكليك اسيد ماشي الحال خدوا بالكم أنا أنا بتكلم + +198 +00:14:57,390 --> 00:15:03,150 +عن nitrogen sources من مصادر natural يعني organic + +199 +00:15:03,150 --> 00:15:07,070 +أفضل ففي إنّي كمان مصادر أخرى inorganic زي الـ + +200 +00:15:07,070 --> 00:15:15,500 +ammonia الأمونيا رمزها NH4 البترات + +201 +00:15:15,500 --> 00:15:18,500 +NO3 مظبوطة ولا لأ؟ ممكن برضه نضيفها، ففي عندي + +202 +00:15:18,500 --> 00:15:23,640 +مصدر نيتروجيني من organic sources و من inorganic + +203 +00:15:23,640 --> 00:15:27,400 +sources، غالبا نحتاج أن نعمل mixture، يعني بنحط + +204 +00:15:27,400 --> 00:15:31,260 +organic مع inorganic، بس الـ carbon source كم واحد؟ + +205 +00:15:32,090 --> 00:15:36,270 +carbon sources one carbon source الـ minerals قلنا + +206 +00:15:36,270 --> 00:15:40,090 +هي عبارة عن تاكرين لما اتكلمنا عن مجموعة من + +207 +00:15:40,090 --> 00:15:44,450 +الكالسيوم و الفوسفرات هاد كلها عناصر تضعف بكميات + +208 +00:15:44,450 --> 00:15:48,330 +قليلة لأن البكتيريا بتكون بحاجتها بالفعل بكميات + +209 +00:15:48,330 --> 00:15:53,510 +قليلة وأعطيكم مثال بسيط على إنها لازم تكون بكميات + +210 +00:15:53,510 --> 00:15:56,250 +قليلة تعرفوا نحاس copper + +211 +00:15:59,750 --> 00:16:06,110 +essential لبعض الـ enzymes في الخلية البكتيرية لكن + +212 +00:16:06,110 --> 00:16:10,690 +الـ copper نفسه النحاس لو زادت كميته بيقتل البكتيريا + +213 +00:16:10,690 --> 00:16:15,330 +واتعرف إن في قطعة معدنية مصنوعة من النحاس مصنوعة + +214 +00:16:15,330 --> 00:16:19,290 +من النحاس لو أخذنا قطعة معدنية مصنوعة من النحاس و + +215 +00:16:19,290 --> 00:16:22,590 +حطيناها على طبق فيه بكتيريا هتلاقي حول البكتيريا + +216 +00:16:22,590 --> 00:16:24,950 +منطقة خالية من مادة البكتيريا + +217 +00:16:27,710 --> 00:16:32,070 +لكن required in trace amount بكميات ضئيلة جدا + +218 +00:16:32,070 --> 00:16:36,810 +النقطة الخاصة اللي قلنا عنها الـ growth factors + +219 +00:16:36,810 --> 00:16:41,050 +that a cell must have for the growth but they + +220 +00:16:41,050 --> 00:16:46,590 +cannot synthesize by themselves بمعنى أنه لازم + +221 +00:16:46,590 --> 00:16:52,490 +أزود البكتيريا بهذه المكونات لأنها ما بتقدر تصنعها + +222 +00:16:52,490 --> 00:16:53,430 +بذلك + +223 +00:16:55,920 --> 00:16:59,500 +من الـ growth factor بدنا نأخذ معلومة باللون الأحمر + +224 +00:16:59,500 --> 00:17:05,400 +معمولة على اللوحة اسمها fastidious bacteria ركزوا + +225 +00:17:05,400 --> 00:17:08,400 +معايا في الجزئية دي لإنه مهم جدا الـ fastidious + +226 +00:17:08,400 --> 00:17:13,520 +bacteria هي بكتيريا تختلف + +227 +00:17:13,520 --> 00:17:16,060 +عن باقي أنواع البكتيريا أو بتعطي مثال أنا حاطط هنا + +228 +00:17:16,060 --> 00:17:21,360 +على اللوحة بكتيريا اسمها hemophilus influenza + +229 +00:17:23,430 --> 00:17:27,710 +الاسم فقط لأنه ظنّ في يوم من الأيام أن هي المسبب للـ + +230 +00:17:27,710 --> 00:17:31,370 +influenza لأنها عُزِلت من بعض الناس اللي كان عندهم + +231 +00:17:31,370 --> 00:17:34,790 +influenza وما كان الفيروس معروف في ذلك الوقت فأطلق + +232 +00:17:34,790 --> 00:17:37,910 +عليها Hemophilus influenza على أن هي المسبب لمين + +233 +00:17:37,910 --> 00:17:41,070 +لمرض الـ influenza وبعد ذلك اكتشفت أن هو طبعا الـ + +234 +00:17:41,070 --> 00:17:46,820 +virus المشهور الآن هذه البكتيريا بتحتاج إلى .. + +235 +00:17:46,820 --> 00:17:51,680 +مابتقدرش تعيش بدون two factors واحد بيسموه X واحد + +236 +00:17:51,680 --> 00:17:57,200 +بيسموه V factor والـ X هو همين والـ V factor هو + +237 +00:17:57,200 --> 00:18:04,320 +الناد فاكتور يبقى في عندي two compounds لازم أحطهم + +238 +00:18:04,320 --> 00:18:09,080 +في الوسط عشان البكتيريا هذه تقدر تم .. مابتقدرش + +239 +00:18:09,080 --> 00:18:13,160 +تصنعهم بدالها مع العلم أن هدول الاثنين other + +240 +00:18:13,160 --> 00:18:18,720 +بكتيريا بتقدر تصنعهم other بكتيريا عادية بتصنعهم + +241 +00:18:18,720 --> 00:18:23,980 +فالـ بكتيريا اللي بتتميز إن إلها حاجة واضحة من الـ + +242 +00:18:23,980 --> 00:18:28,380 +growth factors بنسميها fastidious البعض بيطلق عليها + +243 +00:18:28,380 --> 00:18:31,920 +البكتيريا المدللة من ناحية الغذائية يعني إلها + +244 +00:18:31,920 --> 00:18:37,350 +requirement خاصة من الـ growth factors special amino + +245 +00:18:37,350 --> 00:18:40,330 +acids معينة بنضيف لهم بعض الـ purines وبعض الـ + +246 +00:18:40,330 --> 00:18:43,930 +pyrimidines وبعض الأحيان الـ vitamins في إنّ الـ + +247 +00:18:43,930 --> 00:18:50,330 +Brucella بتحتاج vitamin B لبروسيلا وبالتالي بنسميها + +248 +00:18:50,330 --> 00:18:55,450 +برضه fastidious وغالبا الـ fastidious بتكون slow + +249 +00:18:55,450 --> 00:19:00,500 +growing ماهي slow growing؟ يعني بدل ما تأخذ 24 ساعة + +250 +00:19:00,500 --> 00:19:04,520 +للمستعمرات حتى تظهر، خد بالك، هنتكلم الآن عن + +251 +00:19:04,520 --> 00:19:09,340 +العوامل اللي بتأثر على النمو، صح؟ وبالتالي يعني + +252 +00:19:09,340 --> 00:19:14,280 +أعتقد أنه في نهاية الموضوع مضطرين نتكلم عن نمو + +253 +00:19:14,280 --> 00:19:17,200 +البكتيريا، الآن احنا بنتكلم عن العوامل اللي بتأثر على + +254 +00:19:17,200 --> 00:19:20,100 +النمو التالي، احنا بنتكلم عن نمو itself، احنا + +255 +00:19:20,100 --> 00:19:25,340 +بننمو بالطول، بنجيس طولنا، ممكن نجيس الوزن تبعنا، + +256 +00:19:25,340 --> 00:19:28,300 +طبعًا إن احنا زدنا في الوزن، بس نمو البكتيريا لا + +257 +00:19:28,300 --> 00:19:34,100 +يقاس بأي من المقاييس هذه، يقاس بالعدد، نمو البكتيريا + +258 +00:19:34,100 --> 00:19:43,480 +يقاس بالعدد 2 4 8 16 32 64 128، وها كده اللي هي + +259 +00:19:43,480 --> 00:19:47,580 +اتضاعف لإن الخلية بالـ C تنتهي تنتهي بالـ Zero أربعة + +260 +00:19:47,580 --> 00:19:52,520 +لأنه انقسام ثنائي بصير تبقى بتعبد الأرقام 2 4 8 16 + +261 +00:19:52,520 --> 00:19:56,840 +كده تحفظوهم في كل شيء كمان مرة ما نشتغلش بمصطلح اللي + +262 +00:19:56,840 --> 00:20:00,970 +باللون الأحمر fastidious، ماذا يعني؟ يعني أن + +263 +00:20:00,970 --> 00:20:03,990 +البكتيريا لها requirement خاصة من الـ growth + +264 +00:20:03,990 --> 00:20:08,710 +factors، نجي لعامل ثاني غير الـ nutrient اللي هو + +265 +00:20:08,710 --> 00:20:13,660 +متعلق بالـ temperature، أكيد في التلاجة احنا بنحفظ + +266 +00:20:13,660 --> 00:20:19,300 +الأغذية، ومع ذلك، لو طلعنا من التلاجة أغذية صار + +267 +00:20:19,300 --> 00:20:24,200 +فيها عفن أو فيها فساد زي حليب طلعت له رائحة كريهة + +268 +00:20:24,200 --> 00:20:28,680 +أو شفنا بقع على فاكهة أو خضروات في التلاجة، هذا + +269 +00:20:28,680 --> 00:20:33,180 +مؤشر على إيش؟ على إن المسبب يستطيع إنه في درجات + +270 +00:20:33,180 --> 00:20:36,240 +حرارة منخفضة، لدرجات حرارة التلاجة، هذا قبل حتى + +271 +00:20:36,240 --> 00:20:39,120 +موضوع قطع الكهرباء، هذا عمر طبيعي إنه يصير في فساد + +272 +00:20:39,120 --> 00:20:39,720 +في التلاجة + +273 +00:20:42,440 --> 00:20:46,920 +ننتقل لموضوع ثاني غير تلاجة، هناك بعض الأطعمة تحفظ + +274 +00:20:46,920 --> 00:20:53,740 +ساخنة، كيف تحفظ ساخنة؟ يعني مثلًا عندنا اليوم وليمة + +275 +00:20:53,740 --> 00:21:00,180 +و حضرنا الطعام الساعة أحد عشر، اثنا عشر كان جاهز، لكن + +276 +00:21:00,180 --> 00:21:04,570 +الوليمة الساعة الخامسة مساءً، وبالتالي أنا مضطر أحفظ + +277 +00:21:04,570 --> 00:21:08,370 +الغذاء هذا في درجة حرارة خمسين أو ستين درجة مئوية + +278 +00:21:08,370 --> 00:21:13,010 +عشان يظل ساخن ما يتسخنش برة، آخر شيء لأنه بيفقد بعض + +279 +00:21:13,010 --> 00:21:16,950 +الـ quality السابقة، اللي هو تتسخن و تبرد، هناك بعض + +280 +00:21:16,950 --> 00:21:20,110 +البكتيريا قد تنمو في هذا الغذاء و هتسبب food + +281 +00:21:20,110 --> 00:21:25,310 +poisoning، تمام؟ لإن جوانب دي في التلاجة مش خلصان + +282 +00:21:25,310 --> 00:21:28,570 +وعلى حرارة مرتفعة برضه إيه؟ في أننا ناس أو فينا + +283 +00:21:28,570 --> 00:21:31,690 +مجموعات من البكتيريا عندها القدرة على أنها تنمو + +284 +00:21:31,690 --> 00:21:34,670 +وتماننا نزيزة، طب احنا أجسامنا سبعة و ثلاثين درجة + +285 +00:21:34,670 --> 00:21:38,210 +مئوية اللي هي عالية جدًا وليها مخفضة، وبرضه بنصاد + +286 +00:21:38,210 --> 00:21:42,960 +الالتهابات للبكتيريا، إذن حاجة البكتيريا للحرارة + +287 +00:21:42,960 --> 00:21:46,780 +تختلف من نوع البكتيريا، صنفناها من تصنيف بسيط في + +288 +00:21:46,780 --> 00:21:50,220 +تصنيفات أعقد من هذه، إلى ثلاث أصناف، Psychrophilic محب + +289 +00:21:50,220 --> 00:21:54,360 +للـ برودة، و Mesophilic محب لدرجة حرارة الوسطى، و + +290 +00:21:54,360 --> 00:22:00,580 +Thermophilic المحب للحرارة المرتفعة، 15-20 طبعًا + +291 +00:22:00,580 --> 00:22:03,280 +خدوا بالكم أنا هتكلم عن المصطلح الآن أو تلت + +292 +00:22:03,280 --> 00:22:07,320 +مصطلحاتها، احفظوهم كويس أو فهموهم كويس، الهندية حاسمة + +293 +00:22:07,320 --> 00:22:11,080 +minimum وحاسمة maximum وحاسمة optimum + +294 +00:22:28,860 --> 00:22:37,590 +أفضل نمو هو كلمة optimum، الآن واضح أنه لما أنا عملت + +295 +00:22:37,590 --> 00:22:41,030 +إشارة بالأصابيع، قلت minimum و maximum وفي النص + +296 +00:22:41,030 --> 00:22:48,530 +إيه الـ optimum؟ لا، يعني إن الإصبع الأوسط في النص + +297 +00:22:48,530 --> 00:22:51,730 +إن درجة الحرارة الـ optimum بتكون بين التنتين، قد + +298 +00:22:51,730 --> 00:22:55,450 +تكون قريبة للـ minimum أو قد تكون قريبة للـ maximum + +299 +00:22:55,450 --> 00:23:00,110 +أو قد تكون في المنتصف، يعني ليس هنا قانون بيقول إن + +300 +00:23:00,110 --> 00:23:04,380 +الـ optimum وين يكون موجود، قريب من الـ .. و هنشوف + +301 +00:23:04,380 --> 00:23:07,880 +كمان صورة الآن و نريكوا إن الـ optimum من الـ Escherichia + +302 +00:23:07,880 --> 00:23:13,000 +Coli قريب من الـ maximum، يعني ما فيش يقولنا قانون + +303 +00:23:13,000 --> 00:23:17,900 +بيحكم، بيعتمد على نوع البكتيريا، اتفقنا كم صنف على حسب + +304 +00:23:17,900 --> 00:23:23,240 +الحرارة؟ Psychrophilic, Mesophilic, Thermophilic، و + +305 +00:23:23,240 --> 00:23:26,140 +أنا بقولكوا الكتب مليئة بأرقام مختلفة عن الأرقام + +306 +00:23:26,140 --> 00:23:31,030 +اللي موجودة هنا، فخلينا نتظن بـ one number أو رقم + +307 +00:23:31,030 --> 00:23:35,770 +الموجود أمامنا الآن، الـ 15-20 مش minimum و maximum + +308 +00:23:35,770 --> 00:23:42,290 +الصحيح، الـ optimum تبعهم من 15-20، لكن قد يكون هناك + +309 +00:23:42,290 --> 00:23:44,950 +growth على أربع درجات مئوية، التلاجة بتكون من 2 لـ 8 + +310 +00:23:44,950 --> 00:23:45,510 +يا تلاجة + +311 +00:24:05,870 --> 00:24:09,230 +القائمة اللي أنتم مش شايفينها وأنا بالكاد أستطيع + +312 +00:24:09,230 --> 00:24:14,760 +أن أقرأها، هيبقى عن مجموعة من أسماء البكتيريا بس بدي + +313 +00:24:14,760 --> 00:24:19,560 +أوريكوا أمثلة، Listeria monocytogenes، الـ minimum + +314 +00:24:19,560 --> 00:24:24,860 +تبعها one، يعني تنمو هنا دلوقت بالتلاجة for sure، صح؟ + +315 +00:24:24,860 --> 00:24:31,420 +الـ maximum خمسة وأربعين، الـ optimum يتراوح من + +316 +00:24:31,420 --> 00:24:37,080 +ثلاثين لسبعة وثلاثين، قريب للـ maximum، نعم، في هن دي + +317 +00:24:37,080 --> 00:24:41,060 +الـ Vibrio marinus، و marinus واضح من اسمها بحرية + +318 +00:24:41,060 --> 00:24:46,600 +أربعة برضه بتنمو في تلاجة، بتنمو مش في تلاجة، الـ + +319 +00:24:46,600 --> 00:24:52,880 +optimum ممكن تدهش، طب احنا قلنا الـ minimum أربعة + +320 +00:24:52,880 --> 00:24:55,920 +هل يعني ذلك أنه + +321 +00:25:00,100 --> 00:25:02,800 +عشان تلاتة، اتنين، واحد، مش هتنمو، ولكن أربعة، خمسة، + +322 +00:25:02,800 --> 00:25:06,980 +ستة، واتذكر تلاتة يقولنا من اتنين لتمانية، وبالتالي + +323 +00:25:06,980 --> 00:25:12,000 +فيه إمكانية للنمو، صحيح مش النمو optimum عشان + +324 +00:25:12,000 --> 00:25:14,900 +هيك اللي بنقول احنا الـ low temperature و الـ slow + +325 +00:25:14,900 --> 00:25:18,960 +down، عشان الـ slow down هتبطئ، بدل الطعام ما يفسد + +326 +00:25:18,960 --> 00:25:23,460 +خلال يومين لو كان على سبعة وثلاثين درجة مئوية، بيفسد + +327 +00:25:23,460 --> 00:25:25,360 +مثلًا بعد أسبوع أو أسبوعين + +328 +00:25:31,510 --> 00:25:34,450 +الجدول اللي قدامنا، كمان مرة، بوضح لي الميكروبات الـ + +329 +00:25:34,450 --> 00:25:36,610 +minimum و الـ maximum، طبعًا مش هنحفظهم هدول + +330 +00:25:36,610 --> 00:25:41,830 +الموجودين في أمامنا على اللوان، لكن الشكل اللي + +331 +00:25:41,830 --> 00:25:45,330 +قدامكوا هذا شكل كتير كويس بيفهمنا الفكرة اللي بدأت + +332 +00:25:45,330 --> 00:25:48,950 +فيها أنا ليها الـ minimum و الـ maximum هي طبعًا + +333 +00:25:48,950 --> 00:25:56,190 +درجات الحرارة الـ degrees Celsius، عندي حوالي 9 أو 8 + +334 +00:25:56,190 --> 00:25:59,850 +درجات مئوية below which Escherichia coli will not + +335 +00:25:59,850 --> 00:26:06,620 +grow، أقل من 8 الإشريشيا كولاي مش هتنمو، أكتر من 48 + +336 +00:26:06,620 --> 00:26:11,340 +درجة مئوية لن تنمو البكتيريا، لن تنمو الإشريشيا + +337 +00:26:11,340 --> 00:26:18,160 +كولاي، وين أفضل نمو؟ على 37، 37 كنا كم مرة أقرب + +338 +00:26:18,160 --> 00:26:22,180 +إلى 48 من مين؟ من اللي هو الـ minimum، يعني أقرب + +339 +00:26:22,180 --> 00:26:27,710 +إلى الـ maximum من الـ minimum، وفي هذه النقطة إشارة + +340 +00:26:27,710 --> 00:26:32,930 +واضحة بإنه الـ .. الـ .. الـ .. الـ .. الـ .. + +341 +00:26:32,930 --> 00:26:37,010 +الـ microbial تختلف في حالتها للـ temperature + +342 +00:26:37,010 --> 00:26:40,250 +وبالتالي بدنا نكون عارفين إن معظم الـ medically + +343 +00:26:40,250 --> 00:26:43,130 +important bacteria .. medically important bacteria + +344 +00:26:43,130 --> 00:26:47,750 +can grow at a mesophilic temperature، لإنهم بسببنا + +345 +00:26:47,750 --> 00:26:51,970 +أمراض، وبالتالي جسمنا 37 و الـ optimum بتبعهم بيكون + +346 +00:26:51,970 --> 00:26:58,010 +قريب من هذه الدرجة، نيجي للـ pH، الـ hydronium ion + +347 +00:26:58,010 --> 00:27:02,110 +concentration أو الـ pH زي ما الـ temperature عامل + +348 +00:27:02,110 --> 00:27:08,310 +حاسم في نمو البكتيريا أيضًا، هاد اللي عامل حاسم أيضًا + +349 +00:27:08,310 --> 00:27:13,450 +وقاله minimum وقاله maximum وقاله optimum، عارفين + +350 +00:27:13,450 --> 00:27:17,430 +ليش؟ مين فيكوا درس clinical chemistry؟ + +351 +00:27:19,470 --> 00:27:23,930 +مين عمل فحص الـ glucose؟ أو حد ما وصلوش للفحوصات + +352 +00:27:23,930 --> 00:27:30,230 +هذه في المختبر؟ طيب الإنزيمات .. الإنزيمات احنا + +353 +00:27:30,230 --> 00:27:36,000 +بنعملها فحص على pH محدد، هذا الـ pH هو الـ optimum + +354 +00:27:36,000 --> 00:27:39,220 +معناه إن الحاجة إن في عندها مرضوية minimum وفي عندها + +355 +00:27:39,220 --> 00:27:44,540 +maximum، below which، لذلك لما احنا بنقول إن الـ pH + +356 +00:27:44,540 --> 00:27:48,620 +غير ملائم هو غالبًا غير ملائم لمين؟ ده الإنزيمات + +357 +00:27:48,620 --> 00:27:54,760 +اللي موجودة في الخلية، هناك بعض أنواع من الميكروبات + +358 +00:27:54,760 --> 00:28:00,410 +بتحب الـ pH المتعادل، وفي ميكروبات بتحب الـ pH المنخفض + +359 +00:28:00,410 --> 00:28:03,810 +كلكوا بتعرفوها و بتاكلوها كل يوم، بتاكلوها أو مش كل + +360 +00:28:03,810 --> 00:28:09,630 +يوم البعض منكوا، المخللات، المخلل، المخلل أصلاً تصنيعه + +361 +00:28:09,630 --> 00:28:15,070 +هو عبارة عن بكتيريا بتخفض الـ pH تبع الخضروات اللي + +362 +00:28:15,070 --> 00:28:17,790 +بتعملوها، المخلل زي الخيار على سبيل المثال، بنزل الـ + +363 +00:28:17,790 --> 00:28:24,390 +pH تبعه إلى طعم أو إلى مضاق المقبول لديكم، الحليب + +364 +00:28:24,390 --> 00:28:30,830 +بيتحول للبن، اللبن بيكون حامض، حموضة مقبولة لديكم، + +365 +00:28:30,830 --> 00:28:35,410 +لما بيتزنخ بيسموه حمض، محمد مش معناته إن الحاجة إنه .. + +366 +00:28:35,410 --> 00:28:40,010 +إنه فاسد، محمد معناته إنه درجة الحموضة نزلت .. + +367 +00:28:40,010 --> 00:28:43,790 +يعني .. يعني ارتفعت الحموضة من الـ pH نزل تمام + +368 +00:28:43,790 --> 00:28:48,190 +إلى درجة غير مقبولة ذوقيًا، اللي هو الذوق، فلذلك هو + +369 +00:28:48,190 --> 00:28:52,490 +ليس فساد صحي وإنما فساد ذوقي، بنسميه organoleptic + +370 +00:28:52,490 --> 00:28:55,650 +spoilage، organoleptic spoilage، يعني إن النفس لا + +371 +00:28:55,650 --> 00:29:01,810 +تتدوق بيه إيجابية، تتدوق بيه سالمة، إذا نعرف إنه في + +372 +00:29:01,810 --> 00:29:06,250 +Acidophilic محب للحمض للـ .. للـ pH المقفض، في نموذج + +373 +00:29:06,250 --> 00:29:11,510 +صارخ طبي لبكتيريا بتحب الـ alkaline pH اللي هي الـ + +374 +00:29:11,510 --> 00:29:16,630 +Vibrio، ودبالكوا احنا المعلومات اللي بتدرسها في الـ + +375 +00:29:16,630 --> 00:29:21,550 +microbiology من باب اعرف عدوك، اعرف عدوك لماذا + +376 +00:29:21,550 --> 00:29:26,890 +نتعلم عن أعدائنا أو عن أعدائنا نتعلم عشان احنا + +377 +00:29:26,890 --> 00:29:31,190 +نعرف كيف نتعامل معهم سواء كان بإيجابية + +378 +00:29:31,190 --> 00:29:35,870 +أو بسلبية، في الآخر نتعامل معهم البكتيريا اللي من + +379 +00:29:35,870 --> 00:29:40,810 +نوع Vibrio بتحب الـ alkaline pH احنا مسكنا هذه + +380 +00:29:40,810 --> 00:29:46,290 +المعلومة، تمام؟ واستفدنا منها في أكثر من طريقة، + +381 +00:29:46,290 --> 00:29:52,070 +ناخد الاستفادة الأولى لما أردنا عزل البكتيريا من + +382 +00:29:52,070 --> 00:29:56,930 +الـ stool يحتوي لجرام الواحد على عشر أقصى اثنا عشر + +383 +00:29:56,930 --> 00:30:01,830 +تقريباً بكتيريا لكل جرام و من أنواع مختلفة و بدي + +384 +00:30:01,830 --> 00:30:04,310 +أعزل مايكروب واحد اسمه Vibrio من بين كل + +385 +00:30:04,310 --> 00:30:08,490 +هالمايكروبات المهولة فبروح بحطهم في وسط الـ pH + +386 +00:30:08,490 --> 00:30:13,150 +سبعة alkaline كلهم بموتوا إلا الـ Vibrio يبقى + +387 +00:30:13,150 --> 00:30:16,350 +استفدت من الـ .. استفدت من المعلومة بالـ selective + +388 +00:30:16,350 --> 00:30:21,680 +isolation معي ولا مش معي؟ استفدت معلومة ثانية من + +389 +00:30:21,680 --> 00:30:25,060 +ناحية طبية، أنا مش قادر أغلي الـ ميه في .. ما عنديش + +390 +00:30:25,060 --> 00:30:28,140 +طاقة أغلي الـ ميه للناس و أقول للناس اغليوا الـ ميه، + +391 +00:30:28,140 --> 00:30:31,160 +بنقولهم بطريقة ثانية، بنقولهم ممكن تعصروا ليمون على + +392 +00:30:31,160 --> 00:30:37,240 +كأس الماء قبل شربه، الليمون بيعمل، بيخفض الـ pH و + +393 +00:30:37,240 --> 00:30:43,510 +بيقتل الـ .. الـ vibrio كلها، بيجعل ظروفها صعبة القصد + +394 +00:30:43,510 --> 00:30:48,450 +من الكلام أنه لما بنتعلم المعلومة احنا المعلومة مش + +395 +00:30:48,450 --> 00:30:52,850 +فقط للحفظ وإنما كيف يمكن استغلال هذه المعلومة + +396 +00:30:52,850 --> 00:30:57,130 +تطبيقياً وأنا بجيب أمثلة خلال الشرح لكن أنا أتمنى + +397 +00:30:57,130 --> 00:31:01,130 +أن العقلية تبعتنا العقلية تبعت الطالب تتحول من + +398 +00:31:01,130 --> 00:31:06,920 +متلقي معلومات إلى قدرة تحليلية أنا كيف ممكن استفيد + +399 +00:31:06,920 --> 00:31:10,360 +من هذه المعلومة وأنا أعتقد أن مجال الـ microbiology + +400 +00:31:10,360 --> 00:31:14,480 +من أروع العلوم اللي ممكن الطالب أو اللي بيدرس لي + +401 +00:31:14,480 --> 00:31:19,220 +هذا المادة أنه يستفيد من المعلومات بشكل تطبيقي و + +402 +00:31:19,220 --> 00:31:24,100 +إيجابي معظم الميكروبات اللي بسببنا أمراض هي من + +403 +00:31:24,100 --> 00:31:28,300 +النوع الأول اللي بتحب الـ ph تبع الـ near الـ + +404 +00:31:28,300 --> 00:31:34,560 +neutrality القريب من اللي هو التعادل برضه جدول آخر + +405 +00:31:34,560 --> 00:31:40,000 +يوضح لنا بعض الميكروبات شوفوا مثلاً الميكروب الأول + +406 +00:31:40,000 --> 00:31:43,820 +اسمه Thiobacillus thioxidans الـ minimum pH point + +407 +00:31:43,820 --> 00:31:49,720 +five يعني very very very acidic والـ maximum أربعة + +408 +00:31:49,720 --> 00:31:53,940 +ولسته والـ optimum من اثنين لثلاثة ومن عشرة + +409 +00:31:53,940 --> 00:31:59,760 +في Vibrio عندنا ولا مش Vibrio؟ في اندي هنا واحدة من + +410 +00:31:59,760 --> 00:32:07,080 +المحبات للـ pH الـ minimum pH تبعها خمسة إلى خمسة + +411 +00:32:07,080 --> 00:32:12,060 +وتمانية الـ optimum قريب من الـ neutral الـ maximum من + +412 +00:32:12,060 --> 00:32:17,030 +ثمانية ونصف إلى تسعة في عندي لورع على طول Erwinia + +413 +00:32:17,030 --> 00:32:20,750 +من تسعة وثلاثة، الـ maximum، في عندي كمان + +414 +00:32:20,750 --> 00:32:25,290 +Thiobacillus تسعة، يعني في عندي تنوع أنواع، تنوع + +415 +00:32:25,290 --> 00:32:29,930 +مختلف من الميكروبات، البعض منها .. البعض منها + +416 +00:32:29,930 --> 00:32:34,190 +بيكون مفضل الـ .. الـ .. الـ acidic، البعض الآخر يفضل + +417 +00:32:34,190 --> 00:32:37,690 +الـ .. الـ alkaline، لكن كما ذكرت لكم معظم الـ + +418 +00:32:37,690 --> 00:32:40,770 +medical important bacteria they prefer ash اللي هو + +419 +00:32:40,770 --> 00:32:48,730 +pH near and neutral نجي للعامل الثالث بعد الرابع + +420 +00:32:48,730 --> 00:32:51,790 +أعتقد هذا هو الأول كان الـ nutrient الثاني كان + +421 +00:32:51,790 --> 00:32:53,890 +الحرارة الثالث كان الـ pH الآن هو الـ oxygen + +422 +00:32:53,890 --> 00:32:58,870 +required لو افترضنا افترضنا جدلاً أي واحد وجالك + +423 +00:32:58,870 --> 00:33:05,120 +الـ oxygen سام للبشر طبعاً حتى هيهجموا على طول أنتو و + +424 +00:33:05,120 --> 00:33:10,160 +تقولوا أنه أنت تتكلم كلام غير منطقي البتة لأن احنا + +425 +00:33:10,160 --> 00:33:15,220 +إذا منعنا الـ oxygen بنموت صح؟ فكيف لك أن تصف الـ + +426 +00:33:15,220 --> 00:33:18,660 +oxygen بالمادة السامة للبشر؟ أنا الآن بوضح حالياً + +427 +00:33:18,660 --> 00:33:25,920 +الفكرة وأرجو أنكم تكونوا صبورين لفهم الموضوع الـ + +428 +00:33:25,920 --> 00:33:30,880 +oxygen رمزه O2 صح؟ و O2 يعني ذرة وذرة من الـ + +429 +00:33:30,880 --> 00:33:35,300 +oxygen، ذرتين oxygen الوظيفة اللي بيعملها الاكسجين + +430 +00:33:35,300 --> 00:33:40,240 +في داخل خلايانا احنا أنه it acts as a final + +431 +00:33:40,240 --> 00:33:42,680 +electron acceptor مش يعني final electron + +432 +00:33:42,680 --> 00:33:45,880 +acceptor؟ مستقبل إلكترونات زوج من + +433 +00:33:45,880 --> 00:33:48,900 +الإلكترونات بتطلع من مركب لمركب لمركب لمركب وفي + +434 +00:33:48,900 --> 00:33:52,740 +الآخر لازم واحد يحمله مين اللي بيحمله؟ الاكسجين + +435 +00:33:52,740 --> 00:33:58,800 +وإن هذه العملية عملية صعبة شوية قد تقسم ظهر + +436 +00:33:58,800 --> 00:34:06,490 +الاكسجين هي تشطر فيتحول إلى O و O بنسميها O singlet + +437 +00:34:06,490 --> 00:34:12,350 +singlet واحدة طبيعة الاكسجين ما تقدرش تعيش singlet + +438 +00:34:12,350 --> 00:34:19,490 +في بعض الانزيمات في الخلية بتطول بتشوف مش بعض + +439 +00:34:19,490 --> 00:34:23,910 +الانزيمات حتى لحاله بدون انزيمات الـ O مع الـ H2O + +440 +00:34:23,910 --> 00:34:28,470 +بيكونوله H2O2 hydrogen peroxide الـ hydrogen + +441 +00:34:28,470 --> 00:34:30,110 +peroxide يوصف + +442 +00:34:34,160 --> 00:34:38,280 +Powerful Oxidizing agent يعني هيدمر بعض + +443 +00:34:38,280 --> 00:34:41,600 +الـ molecules في الخلية زي الـ DNA زي الـ RNA زي الـ + +444 +00:34:41,600 --> 00:34:43,460 +protein زي الـ carbohydrate اللي هي بيسموها الـ + +445 +00:34:43,460 --> 00:34:48,420 +macro molecules الجزيئات الكبيرة الـ O الثانية + +446 +00:34:48,420 --> 00:34:52,260 +وراها الـ O الثانية قد تتحول إلى ما يسمى super + +447 +00:34:52,260 --> 00:34:58,150 +oxide يبقى في اندي أو تفاعل مع هيدروجين يعفر مع ماء + +448 +00:34:58,150 --> 00:35:02,730 +وتكون H2O2 وواحدة ثانية اتحولت إلى ما يسمى بالـ + +449 +00:35:02,730 --> 00:35:06,070 +super oxide وكلا هما الـ hydrogen peroxide والـ + +450 +00:35:06,070 --> 00:35:09,250 +super oxide كلا هما very powerful oxidizing agent + +451 +00:35:09,250 --> 00:35:16,230 +بيقتل الخلايا إذا احنا ميتين احنا ميتين بالطريقة + +452 +00:35:16,230 --> 00:35:22,850 +هذه طب ليش احنا مش ميتين؟ لأن الجسم بتاعنا في + +453 +00:35:22,850 --> 00:35:27,630 +system بيحمينا من الـ oxygen toxicity كل ما يتكون + +454 +00:35:27,630 --> 00:35:32,370 +super oxide بيعارب تأثيره هذا الانزيم كل ما يتكون + +455 +00:35:32,370 --> 00:35:35,970 +hydrogen peroxide بيحلله هذا الانزيم هنشوف هذه + +456 +00:35:35,970 --> 00:35:42,650 +الانزيمات بعد قليل لكن بنعرف أن الـ oxygen سام + +457 +00:35:43,850 --> 00:35:47,570 +الاوكسجين ساعة هذا المفهوم الجديد بالنسبة لكم أرجو + +458 +00:35:47,570 --> 00:35:52,170 +أنكم تكونوا فهمتوا ليش احنا وصلنا لهذا المفهوم + +459 +00:35:52,170 --> 00:35:55,070 +اللوحة اللي أمامكم ليس لها علاقة بالمفهوم اللي + +460 +00:35:55,070 --> 00:36:01,830 +حكيته لكن كويس الآن نعرض اللوحة ونشوف الشكل + +461 +00:36:01,830 --> 00:36:06,970 +اللي أمامكم ايش بيعمل لو أنا حطيت بكتيريا في + +462 +00:36:06,970 --> 00:36:12,460 +أنبوبة بكتيريا رهولة قد تتخذ واحد من الخمس أشكال + +463 +00:36:12,460 --> 00:36:17,740 +اللي أمامكم رقم واحد البكتيريا والنامية على السطح + +464 +00:36:17,740 --> 00:36:23,240 +البكتيريا رقم اثنين والنامية بالقرب الأولى هوائية + +465 +00:36:23,240 --> 00:36:29,000 +إجبارية والثانية لا هوائية إختيارية ليش أنا استنتجت + +466 +00:36:29,000 --> 00:36:33,000 +هذا الاستنتاج لأن الأنبوبة بشكل عام أي عمود من + +467 +00:36:33,000 --> 00:36:36,420 +الماء لو جينا نطلع على مستوى الـ oxygen فيه كل ما + +468 +00:36:36,420 --> 00:36:39,900 +نزلنا تحت كل ما قل مستوى الـ oxygen كل ما طلعنا + +469 +00:36:39,900 --> 00:36:44,140 +فوق كل ما زاد مستوى الـ oxygen ففوق بالفعل في رقم + +470 +00:36:44,140 --> 00:36:49,040 +واحد في oxygen نبت البكتيريا تحت فيش oxygen نبت + +471 +00:36:49,040 --> 00:36:52,920 +البكتيريا فهذه بكتيريا لا هوائية لاحظوا ما نامتش + +472 +00:36:52,920 --> 00:36:57,460 +فوق لإنه فيه Oxygen ايش صار فيها؟ ماتت البكتيريا، + +473 +00:36:57,460 --> 00:37:01,980 +اللي فوق ماتت، ماتت لأن الـ Oxygen سام بالنسبة لها، + +474 +00:37:01,980 --> 00:37:06,260 +زي ما احنا سام بالنسبة لنا، بس الفرق بيننا وبينها + +475 +00:37:06,260 --> 00:37:11,000 +أنه هي لا تمتلك ما نمتلك نحن من انزيمات تعادل سمية + +476 +00:37:11,000 --> 00:37:15,480 +الاوكسجين فبتوقع البكتيريا الهوائية يكون نفس الانظمة + +477 +00:37:15,480 --> 00:37:18,100 +اللي عندنا موجودة عندها، بينما البكتيريا اللي + +478 +00:37:18,100 --> 00:37:22,360 +هوائية ما عندها هذه الانظمة رقم ثلاثة، والله + +479 +00:37:22,360 --> 00:37:24,820 +البكتيريا هذا اللي فوق، الثلاثة من ما هي فارقة + +480 +00:37:24,820 --> 00:37:29,400 +معاها صح؟ لا مش فارقة معاها، نامية فوق و نامية + +481 +00:37:29,400 --> 00:37:37,060 +تحت، هتكلم عنها بعد قليل، رقم أربعة، لا والنامية، + +482 +00:37:37,060 --> 00:37:41,550 +على السطح ولا تحت السطح شوية؟ هذه بكتيريا بتحب تشيل + +483 +00:37:41,550 --> 00:37:44,630 +اوكسجين بكميات كبيرة، بتحب بكميات قليلة، بيسموها + +484 +00:37:44,630 --> 00:37:50,330 +micro-aerophilic يعني ترغب بكميات اوكسجين + +485 +00:37:50,330 --> 00:37:53,990 +منخفضة، نسبة الاوكسجين في الجو اللي جاتاش يا + +486 +00:37:53,990 --> 00:37:57,970 +فنات، يعني في الجو العادي، في البيئة اللي احنا + +487 +00:37:57,970 --> 00:38:02,110 +عايشين فيها، جاتاش نسبة الاوكسجين؟ عشرين بالمية، + +488 +00:38:02,110 --> 00:38:06,330 +تقريباً عشرين وتسعة من عشرة، يعني تقريباً واحد وعشرين + +489 +00:38:06,330 --> 00:38:10,880 +بالمية البكتيريا الهوائية العادية اللي في رقم واحد + +490 +00:38:10,880 --> 00:38:16,320 +بتعيش عندها المستوى بدون أي مشاكل رقم أربعة في + +491 +00:38:16,320 --> 00:38:19,800 +اللوحة اللي هي الـ microaerophilic فقط بده خمسة + +492 +00:38:19,800 --> 00:38:24,040 +بالمية فلاحظوا الخمسة بالمية وأنا حصلتهم مش على + +493 +00:38:24,040 --> 00:38:29,070 +السطح مباشرة من تحت السطح رقم خمسة ورقم ثلاثة ايش + +494 +00:38:29,070 --> 00:38:31,870 +الفرق بينهم؟ مين يرفع ايه اللي بيقول ايش الفرق بين + +495 +00:38:31,870 --> 00:38:35,890 +الاثنين؟ ثلاثة هي ثلاثة ما هي هوائية إجبارية لأنه + +496 +00:38:35,890 --> 00:38:39,330 +نموها في كدا يوم بس فوق كان تركيزها أكثر عن + +497 +00:38:39,330 --> 00:38:43,830 +الأكسوجين لكن هناك لا هوائية إختيارية يعني أنه كان + +498 +00:38:43,830 --> 00:38:47,470 +ثانوية في كل النمو بس يعني أنه نموها فاقش كثير + +499 +00:38:47,470 --> 00:38:52,610 +كيف لا هوائية إختيارية يعني؟ بتعيش في ظروف ما فيهاش + +500 +00:38:52,610 --> 00:38:55,490 +.. فيها اوكسوجين وما فيهاش بس بتفضل اللي ما فيهاش + +501 +00:38:55,490 --> 00:38:59,430 +اغتيارية رقم ثلاثة رقم ثلاثة facultative أنا + +502 +00:38:59,430 --> 00:39:02,030 +aerobic وكلامك دقيق مائة بالمائة facultative أنا + +503 +00:39:02,030 --> 00:39:06,610 +aerobic لأنه نمت فوق ونمت تحت لكن نموها في الأعلى + +504 +00:39:06,610 --> 00:39:11,610 +أكثر لإن أنا أفضل aerobic رقم خمسة لا يطلق عليها + +505 +00:39:11,610 --> 00:39:17,760 +اللقب المصطلح اللي أطلقتيه أنت شايف إيه المصطلح؟ لأ + +506 +00:39:17,760 --> 00:39:22,760 +يطلق عليها هوائية تتحمل ولود الأكسجين بمعنى إنه + +507 +00:39:22,760 --> 00:39:29,640 +هي أصلا هوائية لكن فوت قالية لتحمل الأكسجين فهو + +508 +00:39:29,640 --> 00:39:33,840 +بالتالي نمت في كل الأنبوبة بنفس المستوى، خدوا + +509 +00:39:33,840 --> 00:39:38,360 +بالكم، تحمل الشيء غير استخدام الشيء، يعني ما + +510 +00:39:38,360 --> 00:39:44,620 +استخدمت؟ الأكسجين، بينما رقم ثلاثة فوجها ناشع عملت + +511 +00:39:45,920 --> 00:39:51,120 +استخدام الـ oxygen، كمان مرة، خمس أنواع، انسوا + +512 +00:39:51,120 --> 00:39:54,800 +اللوحة، انسوا اللوحة، تعالوا نحفظهم على قاعدة + +513 +00:39:54,800 --> 00:39:59,520 +الإصبع أو الأصابع، هوائية إجبارية، لا هوائية + +514 +00:39:59,520 --> 00:40:03,040 +إجبارية، في المنتصف facultative and aerotolerant، ده + +515 +00:40:03,040 --> 00:40:08,570 +هي بحلقة وصل من الهوائية واللا هوائية مش الأرهانة + +516 +00:40:08,570 --> 00:40:13,090 +هذه microaerophilic هوائية لكن بدها كمية oxygen + +517 +00:40:13,090 --> 00:40:17,870 +قليلة، الرابع اللي قريب من اللي لا هوائية بقولنا لا + +518 +00:40:17,870 --> 00:40:22,130 +هوائية وسميناها aerotolerant anaerobic يعني لا + +519 +00:40:22,130 --> 00:40:26,730 +هوائية ولكن تتحمل وجود الـ oxygen فسنعمل خمسة إن + +520 +00:40:26,730 --> 00:40:31,750 +شاء الله الثالثة + +521 +00:40:31,750 --> 00:40:37,830 +هي بكتيريا هوائية بكتيريا هوائية ماذا يعني الثالثة + +522 +00:40:37,830 --> 00:40:41,370 +البكتيريا الهوائية؟ تستطيع أن تعيش في ورود الهواء + +523 +00:40:41,370 --> 00:40:45,870 +وعدم ورود الهواء تستغل الأكسجين وتستغل مركبات + +524 +00:40:45,870 --> 00:40:50,750 +أخرى في التنفس، تمام؟ لكن خدوا بالكم من شغل، لو + +525 +00:40:50,750 --> 00:40:56,670 +أنا معاه جزئي جلوكوز وأريد أن أعمله oxidation أو + +526 +00:40:56,670 --> 00:41:00,170 +metabolism، متابوليزم دعوني أقول مصطلح أدق، لو + +527 +00:41:00,170 --> 00:41:04,870 +عملت له متابوليزم في ورود الأكسجين، سأحصل على 36 + +528 +00:41:04,870 --> 00:41:10,640 +ATP لو عملت له metabolism في غياب الـ oxygen أحسنها + +529 +00:41:10,640 --> 00:41:17,260 +اثنين اثنين يبقى فعالية الطاقة الناتجة عن جزء + +530 +00:41:17,260 --> 00:41:20,920 +الجلوكوز في وجود الـ oxygen بتكون إيه أفضل لذلك + +531 +00:41:20,920 --> 00:41:24,920 +الفاكولتاتيف أنا إيروف بتفضل النمو الهوائي عشان + +532 +00:41:24,920 --> 00:41:30,040 +أنا لاجيناها فوق أعدادها أكبر من وين من تحت بينما + +533 +00:41:30,040 --> 00:41:33,560 +الـ air tolerant bacteria قلنا لكم مطابق النمو + +534 +00:41:33,560 --> 00:41:39,960 +فيها لأنه ليس الأكسجين دخل في النمو، ليس الأكسجين + +535 +00:41:39,960 --> 00:41:44,180 +أي دخل في النمو وإنما البكتيريا بطبيعتها لا + +536 +00:41:44,180 --> 00:41:48,220 +هوائية، لا تستخدم الأكسجين ولكن طورت قالية لحماية + +537 +00:41:48,220 --> 00:41:55,060 +نفسها من الأكسجين هذا اللوحة اللي أمامكم هي نفس + +538 +00:41:55,060 --> 00:42:01,000 +اللوحة اللي شفتوها بس بالكلام مش بالرسم أنا حكيت عن + +539 +00:42:01,000 --> 00:42:04,560 +الـ Oxygen toxicity concept اللي هي بتكلم عن ال + +540 +00:42:04,560 --> 00:42:09,420 +counter parts اللي هم الـ systems الأنظمة اللي + +541 +00:42:09,420 --> 00:42:16,060 +استخدمت في التخلص من الـ toxicity نتطلع على الدوحة + +542 +00:42:16,060 --> 00:42:23,790 +اللي قدامنا هنا أول معادلة بتقول 2O2 زي 2H2O + +543 +00:42:23,790 --> 00:42:30,530 +باستخدام الانزيم اللي اسمه super oxide dismutase + +544 +00:42:30,530 --> 00:42:37,510 +SOD super oxide dismutase اتحولت إلى H2O2 زائد O2 + +545 +00:42:37,510 --> 00:42:43,310 +لاحظوا شوية الـ 2O2 ها دي اللي فوقيها شحطة هفير + +546 +00:42:43,310 --> 00:42:50,580 +شحطة ها دي صغيرة هذا المركب يسمى super oxide Super + +547 +00:42:50,580 --> 00:42:55,100 +Oxide هذا الإشارة اللي عليه تسمى أو تعطي لقب Super + +548 +00:42:55,100 --> 00:43:00,240 +Oxide طيب الـ Super Oxide Dismutase حول الـ Super + +549 +00:43:00,240 --> 00:43:06,940 +Oxide إلى Hydrogen Peroxide من سم إلى سم، صح؟ هذه + +550 +00:43:06,940 --> 00:43:12,660 +خطوة على الطريق الخطوة الثانية الـ hydrogen peroxide + +551 +00:43:12,660 --> 00:43:16,460 +اللي اتكون سواء من الـ super oxide بسم تايز أو + +552 +00:43:16,460 --> 00:43:20,660 +اللي اتكون لحاله الإسلام دي hydrogen peroxide + +553 +00:43:20,660 --> 00:43:25,400 +إنزيم الكتاليز على طول بياخده وبيحوله إلى ماء زائد + +554 +00:43:25,400 --> 00:43:30,940 +زائد أكسجين يبقى رجعنا الأكسجين زي ما كان تخلصنا + +555 +00:43:30,940 --> 00:43:34,560 +من مين الآن من السمية تبع هذا الأكسجين + +556 +00:43:37,090 --> 00:43:41,810 +طيب في عندي معادلة ثالثة إيش دخلها المعادلة؟ ولو + +557 +00:43:41,810 --> 00:43:47,570 +الثانية خلصه؟ الثالثة إيش دخلها دي؟ خدوا بالكم هناك + +558 +00:43:47,570 --> 00:43:51,570 +بعض المخلوقات لا تحتوي على كتاليز تحتوي على حاجة + +559 +00:43:51,570 --> 00:43:56,130 +اسمها peroxidase الـ peroxidase بيقوم بنفس العمل + +560 +00:43:56,130 --> 00:43:59,010 +اللي بيقوم به الكتاليز لكن بطريقة مختلفة بطريقة + +561 +00:43:59,010 --> 00:44:04,490 +مختلفة أعطيكم مثال هنا فلو أنا اشتري عندي هنا 2H2O + +562 +00:44:04,490 --> 00:44:12,220 +في المعادلة هنا الـ H2O2 بيعطيها لمركب لمركب مختزل + +563 +00:44:12,220 --> 00:44:18,820 +مركب مختزل بوجود البروكسدايز بطلع ماء زي المركب + +564 +00:44:18,820 --> 00:44:23,420 +مؤكسد رحمة + +565 +00:44:23,420 --> 00:44:27,280 +الهيدروجين راح منه الهيدروجين فصار لنا إيه؟ مؤكسد + +566 +00:44:27,280 --> 00:44:33,480 +لكن ما طلعش Oxygen بمعنى آخر المحصلة النهائية ال + +567 +00:44:33,480 --> 00:44:37,680 +hydrogen peroxide في الثانية اتحول لمين؟ لماء في + +568 +00:44:37,680 --> 00:44:43,100 +الأولى اتحول لماء زائد Oxygen وفي التالي اتحول ماء + +569 +00:44:43,100 --> 00:44:48,960 +إلى مركب مؤكسد بجكال مختصر واتأكسد في الحالتين + +570 +00:44:48,960 --> 00:44:55,330 +خلصنا من سمية الـ hydrogen peroxide طبعا هذه نقطة + +571 +00:44:55,330 --> 00:44:59,130 +يجب أن نجد في عينها دقيقة واحدة عشان نقول لكم إيش + +572 +00:44:59,130 --> 00:45:04,210 +ممكن أستفيد منها تتذكروا الـ gram-positive cocci؟ + +573 +00:45:04,210 --> 00:45:08,450 +gram-positive بكتيريا لما تكون البكتيريا شكلًا + +574 +00:45:08,450 --> 00:45:13,790 +كوكساي بنقول gram-positive cocci كروي يعني من أشهر + +575 +00:45:13,790 --> 00:45:18,130 +الأسماء اللي هتعرفوها أو عرفتوها is staphylococcus + +576 +00:45:18,130 --> 00:45:22,940 +aureus ومن أشهر الميكروفات اللي أنتم مرضتوا فيها + +577 +00:45:22,940 --> 00:45:27,860 +في يوم من الأيام باللواز، Penicilitis اسمها + +578 +00:45:27,860 --> 00:45:33,300 +streptococcus, adenostepilococcus و streptococcus + +579 +00:45:33,300 --> 00:45:37,880 +الاثنين جرام بوزيتيف كوكساية، أحد أهم الفحوصات + +580 +00:45:37,880 --> 00:45:43,770 +المميزة بين الاثنين فحص الكتاليز الستافيلوكوكس + +581 +00:45:43,770 --> 00:45:49,890 +كتاليز positive بتطلع كتاليز بينما الستريبتوكوكس + +582 +00:45:49,890 --> 00:45:55,190 +كتاليز negative تعالوا كيف نعمل فحص لكي تكونوا + +583 +00:45:55,190 --> 00:46:00,030 +عارفين أن كل معلومة بندرسها هنا لها تطبيق في + +584 +00:46:00,030 --> 00:46:04,770 +المعمل هاي شريحة بنجيب قطرة من الـ hydrogen + +585 +00:46:04,770 --> 00:46:10,250 +peroxide بنحط على القطرة البكتيريا اللي بنفحصها + +586 +00:46:11,160 --> 00:46:16,760 +الخلايا البكتيريا إذا فيها كتاليز هيتفاعل مع ال + +587 +00:46:16,760 --> 00:46:19,720 +hydrogen peroxide ويحوله بالمعادلة اللي بالدم وفي + +588 +00:46:19,720 --> 00:46:24,640 +الوسط هنا إلى ماء زائد مين؟ أكسجين، الأكسجين + +589 +00:46:24,640 --> 00:46:28,440 +بيطلع من وين؟ بيطلع من ماء جاعد اللي هو الـ hydrogen + +590 +00:46:28,440 --> 00:46:34,640 +peroxide، فهيطلع بشكل مين؟ بشكل فقاعة الفحص positive + +591 +00:46:34,640 --> 00:46:37,480 +إذا كان تكون في قاعك، الفحص negative إذا لم + +592 +00:46:37,480 --> 00:46:41,380 +يتكون في قاعك، تفهمين إيه السهل الفحص؟ one minute + +593 +00:46:41,380 --> 00:46:47,820 +test لكن .. لكن وظائفه وأهميته كبيرة جدا، ميزة بين + +594 +00:46:47,820 --> 00:46:51,180 +أستاذ فليك وكاي وبين أستاذ فليك وكاي، وطبعا + +595 +00:46:51,180 --> 00:46:55,540 +التفاصيل الأخرى هتعلموها بن وعمل إن شاء الله طيب + +596 +00:46:55,540 --> 00:47:00,260 +هنا يعني .. بالنقطة الأخيرة غطينا العوامل اللي + +597 +00:47:00,260 --> 00:47:06,340 +بتأثر على نمو البكتيريا البكتيريا تنقسم أو تتكاثر + +598 +00:47:06,340 --> 00:47:09,760 +بالـ simple binary fission شوفوا الشكل اللي قدامكم + +599 +00:47:09,760 --> 00:47:14,700 +هذا المادة الوراثية في البكتيريا اللي هو الكروموزوم + +600 +00:47:14,700 --> 00:47:20,640 +صح انقسمت في البداية وكل واحد أخد طرف كل كروموزوم + +601 +00:47:20,640 --> 00:47:26,280 +أخد طرف وبدأ يتكون سبتا سبتا حاجز أو septum تمام و + +602 +00:47:26,280 --> 00:47:32,080 +بدأ يتكون حاجز الحاجز اجتمل وبعدين صار في انقسام + +603 +00:47:32,080 --> 00:47:39,260 +خلية تعمل مين الأم اللي ولدت؟ أي واحدة منهم؟ هاد + +604 +00:47:39,260 --> 00:47:40,280 +الأم ولا هاد الأم؟ + +605 +00:47:45,440 --> 00:47:51,080 +two daughter cells يعني كل واحدة منهم ماخدة من + +606 +00:47:51,080 --> 00:47:55,040 +القديم وماخدة منها من الجديد two daughter cells + +607 +00:47:55,040 --> 00:48:00,080 +هذا انقسام هذه الصورة بتوضح لنا يعني بطريقة رائعة + +608 +00:48:00,080 --> 00:48:05,500 +جدا أن كيف الـ chromosome بدأ يتجري إلى طرف من + +609 +00:48:05,500 --> 00:48:09,840 +طرفين الخلية وهذه المنطقة إذا شفتوا هذه الـ cross + +610 +00:48:09,840 --> 00:48:13,420 +wall أو الـ septum بدأت تظهر في المنطقة هذه في + +611 +00:48:13,420 --> 00:48:14,380 +المنطقة هذه + +612 +00:48:17,080 --> 00:48:20,960 +إذا ما هي وسيلة الانقسام هي الـ simple binary fission + +613 +00:48:20,960 --> 00:48:24,140 +وقلنا ببساطة إن الـ chromosome بينقسم ويروح على + +614 +00:48:24,140 --> 00:48:26,800 +الجهة وعلى الجهة وبعدين يتكون حاجز طبعا بعض + +615 +00:48:26,800 --> 00:48:29,940 +البكتيريا بيبقوا متصلة ببعضها okay بتنقسم لكن + +616 +00:48:29,940 --> 00:48:34,840 +بيبقوا متصلة والانقسام أو عدم الاتصال اللي بيحدده + +617 +00:48:34,840 --> 00:48:38,920 +الـ genetic material اللي موجودة في الخلية شوفتم + +618 +00:48:38,920 --> 00:48:39,700 +مرة دبلو كوكاي + +619 +00:48:49,520 --> 00:48:55,340 +الزمن اللازم للانقسام أو للتضاعف يسمى generation + +620 +00:48:55,340 --> 00:48:58,340 +time أو doubling time + +621 +00:49:07,960 --> 00:49:11,120 +ماني كومون بكتيريا الـ generation time تبقى من + +622 +00:49:11,120 --> 00:49:17,900 +عشرين إلى ستين دقيقة تخيلوا بنات في الساعة تنقسم + +623 +00:49:17,900 --> 00:49:24,080 +البكتيريا ثلاث مرات طيب بدي أبيع سيارتي أنا اليوم + +624 +00:49:24,080 --> 00:49:29,480 +مين يشتريها أعطيكم + +625 +00:49:29,480 --> 00:49:35,020 +عرض تبع السيارة بدي أخد في اليوم الأول منكم شكل + +626 +00:49:35,020 --> 00:49:38,140 +اللي بتشتريه هبدأ أعطيني شاكل و اليوم الثاني بس + +627 +00:49:38,140 --> 00:49:44,440 +يضعف النبلة لـ إثنين شاكل و ثلاثين يوم ثلاثين يوم و + +628 +00:49:44,440 --> 00:49:47,340 +تصوّر أنه في اليوم الأول بدي أخد شاكل و الثاني يوم + +629 +00:49:47,340 --> 00:49:51,560 +بدي أخد كمان إثنين تلت يوم أربعة لـ ثلاثين يوم مين + +630 +00:49:51,560 --> 00:49:57,000 +يشتري اتفقنا بدأت اشتري .. خلاص اتفقنا على النبلة + +631 +00:49:58,230 --> 00:50:02,370 +راتبك من هنا لخمسين سنة إن شاء الله بت .. بت .. + +632 +00:50:02,370 --> 00:50:07,310 +لأنه احسبوا الرقم هتلاقوه رقم مهول جدا بقى إثنين + +633 +00:50:07,310 --> 00:50:10,390 +أص ثلاثين إثنين أص ثلاثين اللي مع أقل حسبة ممكن + +634 +00:50:10,390 --> 00:50:13,850 +تطلع عليه و تشوف إثنين أص ثلاثين كدهش بيطلع الرقم + +635 +00:50:13,850 --> 00:50:19,230 +البكتيريا بتتضاعف في الساعة الواحدة بعض نوع من + +636 +00:50:19,230 --> 00:50:24,190 +البكتيريا ثلاث مرات فخلال أربع وعشرين ساعة أربع + +637 +00:50:24,190 --> 00:50:29,420 +وعشرين في ثلاثة إثنين وسبعين 72 مرة حطوا 2 قص 72 + +638 +00:50:29,420 --> 00:50:33,080 +شوفوا الرقم اللي بيطلع مليون .. بلايين من الـ micro + +639 +00:50:33,080 --> 00:50:35,860 +organism وبالتالي احنا لما نزرع البكتيريا على + +640 +00:50:35,860 --> 00:50:40,220 +الطبق نزرع خلية واحدة غريبة نزرع خلية واحدة بعد 24 + +641 +00:50:40,220 --> 00:50:44,420 +ساعة بنصير نشوف المستعمرات طب البكتيريا حجمها واحد + +642 +00:50:44,420 --> 00:50:47,720 +مايكرون عشان أنا أشوف العادات بتكون بالملي مترات + +643 +00:50:47,720 --> 00:50:50,780 +تمام؟ فعشان أساسي الملي مترات لازم تكون العادات + +644 +00:50:50,780 --> 00:50:56,520 +بالبلايين بالبلايين تمام؟ فواضح أنه زمن الانقسام + +645 +00:50:56,520 --> 00:51:00,400 +في البكتيريا طبعا بتأثر بعوامل كثيرة فاكرين الظروف + +646 +00:51:00,400 --> 00:51:03,180 +اللي حكينا؟ لو أن الـ temperature عشرة أو الـ + +647 +00:51:03,180 --> 00:51:05,740 +temperature خمسة عشر أعتقد الـ generation time + +648 +00:51:05,740 --> 00:51:10,840 +هيختلف لأنه بيأثر على مين؟ على النمو مش معقول أقول + +649 +00:51:10,840 --> 00:51:13,280 +generation time لـ minimum و generation time لـ + +650 +00:51:13,280 --> 00:51:17,380 +maximum الـ generation time غالبا بنقول لـ مين؟ للـ + +651 +00:51:17,380 --> 00:51:22,340 +optimum يعني أفضل الظروف بنوفرها للبكتيريا و بنحسب + +652 +00:51:22,340 --> 00:51:26,400 +الـ generation time و أقول لكم أنه في بعض أنواع من + +653 +00:51:26,400 --> 00:51:30,140 +البكتيريا بنختمها للمعلومة الـ generation time قد + +654 +00:51:30,140 --> 00:51:34,120 +يصل إلى فقط عشر دقائق و نكمل المرة القادمة diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/PLnuv8-yAaY_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/PLnuv8-yAaY_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..ce94bf494d1632bd96c720c3121147bcc6c3ccc0 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/PLnuv8-yAaY_postprocess.srt @@ -0,0 +1,2616 @@ +1 +00:00:21,280 --> 00:00:24,860 +بسم الله الرحمن الرحيم هتكلم عن ال bacterial + +2 +00:00:24,860 --> 00:00:32,400 +physiology و هي مناسبة للموضوع + +3 +00:00:32,400 --> 00:00:35,420 +اللي أنهيناه اللي هو ال bacterial cell structure + +4 +00:00:35,420 --> 00:00:38,040 +وبالتالي من المناسب انه نبدأ ان شاء الله المخاطرة + +5 +00:00:38,040 --> 00:00:40,380 +بأنواع ال bacterial physiology + +6 +00:00:43,590 --> 00:00:47,450 +كأي خلية في الدنيا هناك مكونات لهذه الخلية غالبا + +7 +00:00:47,450 --> 00:00:51,510 +نستطيع أنه نصنفها بتصنيفات مختلفة مثلا ك chemical + +8 +00:00:51,510 --> 00:00:55,570 +component فيها ال water لكن ال water بدنا نغير + +9 +00:00:55,570 --> 00:00:58,790 +مفهومنا عنه اليوم نتكلم عنه علم ال water free + +10 +00:00:58,790 --> 00:01:04,690 +water و bound water يعني قد نرى بعض الأطعمة على + +11 +00:01:04,690 --> 00:01:08,190 +سبيل المثال المربع المربع بحتوي كمية كبيرة من + +12 +00:01:08,190 --> 00:01:12,760 +الماءJelly-like يعني واضح أنه من القوام تبعه أنه + +13 +00:01:12,760 --> 00:01:16,740 +هيحتفظ على كمية كبيرة من الماء لكن لو وضعنا + +14 +00:01:16,740 --> 00:01:21,160 +بكتيريا في ال jelly هلاجئ أنه البكتيريا غير قادرة + +15 +00:01:21,160 --> 00:01:26,180 +على النمو بالعكس الماء اللي جواها بتفقد لصالح مين؟ + +16 +00:01:26,180 --> 00:01:30,020 +المربّى لأنه تركيز السكر عال جدا فيه فهذا يعتبر + +17 +00:01:30,020 --> 00:01:35,530 +bound water يعني ماء مرتبط غير متاح للبكتيرياالماء + +18 +00:01:35,530 --> 00:01:38,530 +المتاح اللي هو ال available water او ال free water + +19 +00:01:38,530 --> 00:01:42,730 +و يعبر عنه بما يسمى ال water activity water + +20 +00:01:42,730 --> 00:01:47,990 +activity شايفين الرمز هذا ال AWهذا بيعبر عن ما + +21 +00:01:47,990 --> 00:01:52,490 +يسمى بال water activity inorganic salts أملاح غير + +22 +00:01:52,490 --> 00:01:55,410 +عضوية زي الفوسفلاس والمغنيسيوم والكالسيوم + +23 +00:01:55,410 --> 00:02:01,450 +والسوديوم لبروتينات واتشكل كمية كبيرة من الوزن + +24 +00:02:01,450 --> 00:02:05,490 +الجاف للخلية حوالي خمسين ل تمانين بالمائة من وزن + +25 +00:02:05,490 --> 00:02:08,790 +الخلية الجاف وعبارة عن بروتينات وانا بتتصوروا + +26 +00:02:08,790 --> 00:02:12,620 +البروتينات مما تتكوّنبتكول لبروتينات من كربون و من + +27 +00:02:12,620 --> 00:02:15,840 +نيتروجين و من سلفر و من other components يعني + +28 +00:02:15,840 --> 00:02:21,380 +البروتين كمركب يشتمل على مركبات أخرى ال sugar أو + +29 +00:02:21,380 --> 00:02:28,580 +السكريات و أذكركم فقط بقضية ال .. ال .. ال .. ال + +30 +00:02:28,580 --> 00:02:32,120 +بتيدوجلايكان ال بتيدوجلايكان أصلا مكون من + +31 +00:02:32,120 --> 00:02:36,020 +inacetylglucosamine و inacetylmuramicacid والتنين + +32 +00:02:36,020 --> 00:02:41,180 +عبارة عن carbohydrates أو sugarsأيضا ال capsule + +33 +00:02:41,180 --> 00:02:44,560 +اللى بتتذكره المكونة كانت من polysaccharide كان + +34 +00:02:44,560 --> 00:02:49,380 +فينا ال lipids او الدهون فاكرين ال phospholipids + +35 +00:02:49,380 --> 00:02:54,620 +ال cell membrane و كان في ال outer membrane تبع ال + +36 +00:02:54,620 --> 00:02:57,360 +gram negative بكتيريا برضه كان فينا outer membrane + +37 +00:02:57,360 --> 00:03:02,000 +و مكون من اللي هو ال lipids طبعا في بعض البكتيريا + +38 +00:03:02,000 --> 00:03:05,980 +فيها fats أخرى بتكون فيها waxy lipids + +39 +00:03:09,380 --> 00:03:12,860 +دهون شمية، و تذكر هذه الجملة كويس لإنه هنستفيد + +40 +00:03:12,860 --> 00:03:16,140 +منها لما نتكلم عن ال acid fast stain، هناك بعض + +41 +00:03:16,140 --> 00:03:18,880 +أنواع من الباتيريا غير قابلة للصداقة بال gram + +42 +00:03:18,880 --> 00:03:23,340 +stainوبالتالي بدنا نعرف السبب ليش غير قابلة + +43 +00:03:23,340 --> 00:03:28,360 +للصباغة نظرا لوجود طبقة من ال waxy lipids في هذه + +44 +00:03:28,360 --> 00:03:31,500 +الخلايا طبعا في اندي كمان nucleic acids و ال + +45 +00:03:31,500 --> 00:03:36,080 +nucleic acid هي عبارة عن RNA and DNA بعكس + +46 +00:03:36,080 --> 00:03:39,720 +الفيروسات اللي ما تحدثنا كنا الفيروسات يا إما DNA + +47 +00:03:39,720 --> 00:03:41,320 +يا إما RNA + +48 +00:03:44,200 --> 00:03:49,160 +نظرة أخرى بطريقة أخرى على البكتيريا ياريتنا نركز + +49 +00:03:49,160 --> 00:03:53,380 +على اللوحة و نشوف أنه اللوحة بتقولنا أنه 50% من + +50 +00:03:53,380 --> 00:04:00,410 +وزن البكتيريا اللي جاء هو carbon60% ل 80% البروتين + +51 +00:04:00,410 --> 00:04:05,010 +موضوع كله البروتين تعبير اخر مختلف عن كلمة carbon + +52 +00:04:05,010 --> 00:04:08,510 +البروتين يحتوي على carbon و غير ال carbon لكن هنا + +53 +00:04:08,510 --> 00:04:11,290 +نتكلم عن ال carbon و ال carbon قد يكون موجود في ال + +54 +00:04:11,290 --> 00:04:14,310 +carbohydrates و في ال limits و في ال proteins في + +55 +00:04:14,310 --> 00:04:17,910 +عندي ال oxygen بيحتوي برضه او نسبة كبيرة من الخلية + +56 +00:04:17,910 --> 00:04:21,750 +هي عبارة عن oxygen في عندي اللي هو ال nitrogen + +57 +00:04:21,750 --> 00:04:26,950 +كمان بشكل 14% من الخليةفي حين دي الهيدروجين تمانية + +58 +00:04:26,950 --> 00:04:30,110 +في المية، في حين دي الفوسفوراس، وفي حين السلفر + +59 +00:04:30,110 --> 00:04:34,630 +والبوتاسيوم والصوديوم بكميات أقل، إذا احنا متفقين + +60 +00:04:34,630 --> 00:04:40,050 +على إنه تركيب الخلية تركيب الخلية قد ينظر إلى + +61 +00:04:40,050 --> 00:04:44,890 +نظرات مختلفة وبأوجه مختلفة، وشوفنا أرقام اختلفت من + +62 +00:04:44,890 --> 00:04:49,420 +لوحة إلى لوحةماعرف اللي هو حين كان اللي أمامكوا + +63 +00:04:49,420 --> 00:04:51,860 +الصغيرة اللي الخطفية الصغيرة ان هي أبقى عنصورة و + +64 +00:04:51,860 --> 00:04:56,120 +احنا يعني فرضناها خالص فصعب الرؤية لكن هحاول ان + +65 +00:04:56,120 --> 00:05:01,100 +اخدر الإمكان انه اذكر او مرور سريع على المراكبات و + +66 +00:05:01,100 --> 00:05:05,300 +اقولكوا المراكبات هذه وين موجودة فعشان بالمثال ان + +67 +00:05:05,300 --> 00:05:08,410 +ال carbonأنا قلت الكربون مولود في وين؟ في + +68 +00:05:08,410 --> 00:05:10,990 +البروتين، مولود في الكربوهادرياس، مولود في + +69 +00:05:10,990 --> 00:05:14,950 +الليبتز، موجود حتى في النوكليك أسيت وبالتالي + +70 +00:05:14,950 --> 00:05:18,490 +الكربون هو أكتر أنصر و احنا اعطيناها خمسين بالمية + +71 +00:05:18,490 --> 00:05:23,810 +من وزن الخلية الجاف، هنا طبعا انا مهم جدا نعرف + +72 +00:05:23,810 --> 00:05:26,910 +الكربون وين موجود ومهم جدا نعرف وين النيترويدين + +73 +00:05:26,910 --> 00:05:30,250 +مولود، اللي عارفين ليش؟ لأن بكرا هنقول انه في حد + +74 +00:05:30,250 --> 00:05:35,960 +ال media، وسط الزراعي، هذا الوسط الزراعيبدنا نوفر + +75 +00:05:35,960 --> 00:05:40,760 +للبكتيريا اللى بنناميها مصادر كربونية مصادر + +76 +00:05:40,760 --> 00:05:43,840 +نيتروجينية بدنا نوفر لها minerals بدنا نوفر لها + +77 +00:05:43,840 --> 00:05:48,380 +water water activity كافية مناسبة فمعرفتي + +78 +00:05:48,380 --> 00:05:53,400 +بالمركبات المحتوية على تلك العناصر بد انا اصنع ال + +79 +00:05:53,400 --> 00:05:56,540 +media تبعتي بدون هذه المعرفة لن استطيع ان اقوم + +80 +00:05:56,540 --> 00:06:00,920 +بتصنية اللى هو ال media فمهم جدا ان اعرف الكلامعند + +81 +00:06:00,920 --> 00:06:05,380 +الأكسجين والأهم مصدر له في الخلية هو مين ال water + +82 +00:06:05,380 --> 00:06:10,980 +ال H2O كلكم بتعرفوا أن الوزن الرطب للخلية قد يشكل + +83 +00:06:10,980 --> 00:06:17,920 +70% ال water من الواق الخلية ال nitrogen والنيترجن + +84 +00:06:17,920 --> 00:06:22,700 +أذكركم بمراكبات بتعرفوها كويس و بتحتوى على نيترجن + +85 +00:06:22,700 --> 00:06:27,250 +واحدماذا يسمى ال .. ال .. ال .. ال .. ال + +86 +00:06:27,250 --> 00:06:30,870 +nitrogenous bases قواعد النيتروجينية اللي هم + +87 +00:06:30,870 --> 00:06:36,150 +الأدينين والجوانين والسايدوسين واليوراسين + +88 +00:06:36,150 --> 00:06:41,310 +والثايمين نعم هدولة كلهم قواعد نيتروجينية ال amino + +89 +00:06:41,310 --> 00:06:44,930 +acids اسمه amino acid لإنه فيه amino group و ال + +90 +00:06:44,930 --> 00:06:49,010 +amino group هو ال H2 مظبوط ال H2 يبقى ال nitrogen + +91 +00:06:49,010 --> 00:06:52,110 +موجود في ال amino acids يعني في البروتيناتإذا + +92 +00:06:52,110 --> 00:06:55,550 +قواعد نيتروجينية و protein و amino acids هذي + +93 +00:06:55,550 --> 00:06:59,790 +المصدر الأيسرانيين للنيتروجين فينا ال .. ال + +94 +00:06:59,790 --> 00:07:03,110 +hydrogen برضه المصدر الأساسي اللي هو الماء لإن هو + +95 +00:07:03,110 --> 00:07:08,720 +للوكسيجين و لل .. فينا ال phosphorusفوسفرس تذكروا + +96 +00:07:08,720 --> 00:07:12,920 +ان ال backbone تبع المادة الوراثية الو ديانات في + +97 +00:07:12,920 --> 00:07:17,340 +فوسفرس في phosphate group في phosphate group تذكر + +98 +00:07:17,340 --> 00:07:20,940 +ال phospholipids اسمها phospholipids يبقى فيها + +99 +00:07:20,940 --> 00:07:27,630 +انها ايضا phosphate groupالسلفر و اذكركم ان السلفر + +100 +00:07:27,630 --> 00:07:33,610 +اسمها sulfur containing amino acids يعني التصنيف + +101 +00:07:33,610 --> 00:07:37,290 +من تصنيفات ال amino acids ان فيها كبريه خدوا بالكم + +102 +00:07:37,290 --> 00:07:42,710 +هذه الكميات و المصادر قد تختلف من microب ل microب + +103 +00:07:42,710 --> 00:07:49,730 +بمعنى بكتيريا تعيش في قاع المحيطات لا تمتلك oxygen + +104 +00:07:49,730 --> 00:07:55,390 +molecular جزائي البتةلأ تبتلك حتى إضاءة عارفين + +105 +00:07:55,390 --> 00:07:58,230 +الأنصر الأساسي إيه لها التنفس هو ال sulfur + +106 +00:07:58,230 --> 00:08:02,990 +الكابريد فهي بحاجة إلى كميات كبيرة من من الكابريد + +107 +00:08:02,990 --> 00:08:06,250 +بالنسبة للبكتيريا العادية الممرضة لأنها حالتها + +108 +00:08:06,250 --> 00:08:10,210 +الكابريد بتكون حاجة قليلة جدا حاجة قليلة جدا + +109 +00:08:11,160 --> 00:08:14,820 +البوتاسيوم والبوتاسيوم عبارة عن كوفاكتور ايضا كمان + +110 +00:08:14,820 --> 00:08:18,200 +المجنيسيوم والكالسيوم والايرون كلهم هدولة نحتاجهم + +111 +00:08:18,200 --> 00:08:21,380 +بكميات قليلة وبيعملوا ك كوفاكتور وممكن يكونوا + +112 +00:08:21,380 --> 00:08:25,420 +موجودين في انزايم محددين واشهر مثال الايرون + +113 +00:08:25,420 --> 00:08:29,060 +الايرون موجود في مجموعة من الانزيمات اسمها + +114 +00:08:29,060 --> 00:08:35,420 +cytochromes والcytochromes هي مجموعة الانزيمات + +115 +00:08:35,420 --> 00:08:42,280 +اللازمة لعملية الاكسدة في الخلية وانتاج الطباللوحة + +116 +00:08:42,280 --> 00:08:48,440 +اللي أمامنا الآن لوحة معبرة وفيها حجم معلومات ضخم + +117 +00:08:48,440 --> 00:08:56,780 +جدا يمكن توقف عندها فترات طويلة لأن كل نقطة من هذه + +118 +00:08:56,780 --> 00:09:00,940 +النقاط يعني هي بحد ذاتها قد تكون فصل او فاصلين في + +119 +00:09:00,940 --> 00:09:04,620 +كتاب ال nutrients ال temperature ال hydrogen ion + +120 +00:09:04,620 --> 00:09:08,020 +concentration او ال VH ال oxygen requirement وال + +121 +00:09:08,020 --> 00:09:14,530 +osmotic pressureناخد واحدة واحدة ونحاول انه بقدر + +122 +00:09:14,530 --> 00:09:17,330 +المقتصر المفيد لان احنا قلنا essential + +123 +00:09:17,330 --> 00:09:21,790 +microbiology نحاول نقلل من حجم المعلومات لكن نعطي + +124 +00:09:21,790 --> 00:09:24,010 +معلومات مفيدة اننا في ال diagnostic microbiome ان + +125 +00:09:24,010 --> 00:09:31,090 +شاء الله ناخد اول معلومة ليها ال nutrients احنا + +126 +00:09:31,090 --> 00:09:38,240 +ممكن نصنف البكتيريا بحسب حاجتها للغذاءأو بطريقتها + +127 +00:09:38,240 --> 00:09:43,060 +في إنتاج أو استهلاك الغذاء إلى سنتين Autotroph ده + +128 +00:09:43,060 --> 00:09:47,880 +دي التغبية وهي يعني لن نتطرق لها في ال medical + +129 +00:09:47,880 --> 00:09:51,440 +microbiology لأن كل ميكروباتنا احنا من نوع تاني + +130 +00:09:51,440 --> 00:09:55,440 +اللي هي ال heterotroph و ال heterotroph they + +131 +00:09:55,440 --> 00:10:02,140 +utilize ready made food يعني أغبية معدة سلفا معدة + +132 +00:10:02,140 --> 00:10:06,740 +و جاهزة من الآخرين ال autotrophs تشبه النباتات + +133 +00:10:07,550 --> 00:10:12,070 +Photosynthetic بتصنع من الإضاءة و الـCO2 + +134 +00:10:12,070 --> 00:10:17,190 +Carbohydrates تمام؟ فهي بيسموها Primary Producers، + +135 +00:10:17,190 --> 00:10:21,530 +منتجة، يعني مخلوقات منتجة، اللي أهميتها البيئية في + +136 +00:10:21,530 --> 00:10:24,970 +كوكب الأرض لكن من ناحية طبيعية ليس لنا بها أي + +137 +00:10:24,970 --> 00:10:29,850 +علاقة، حتى الـHeterotroph بنصنفهم إلى صينفين + +138 +00:10:31,090 --> 00:10:37,070 +Saprophytes وهي باللغة العربية مترمنة وكلمة مترمنة + +139 +00:10:37,070 --> 00:10:42,170 +تطلق على الكائنات اللى تتغذى على مخلوقات ميتة على + +140 +00:10:42,170 --> 00:10:46,750 +مخلوقات ميتة النوع التانى اللى هو البرازيتك او + +141 +00:10:46,750 --> 00:10:49,950 +البرازايتس وهى اللى بتطفل على المخلوقات الحياة + +142 +00:10:49,950 --> 00:10:56,060 +اللى بتشمل الميكروبات اللى بتضرنا احنا كبشرإذا + +143 +00:10:56,060 --> 00:10:59,980 +كمان مرة Autotroph وHeterotroph وHeterotroph تسمان + +144 +00:10:59,980 --> 00:11:03,980 +اللي هم ال Sabrophytes والتاني يقولنا ال parasites + +145 +00:11:03,980 --> 00:11:07,340 +طيب + +146 +00:11:07,340 --> 00:11:10,920 +ال nutrients ال requirement بشكل عام للبكتيريا + +147 +00:11:10,920 --> 00:11:14,740 +تتلخص في مجموعة من العناصر الرئيسية ال water + +148 +00:11:14,740 --> 00:11:21,460 +وتخيلوا إنه قد أحتاج إلى تسعين بالمية من المكونات + +149 +00:11:21,460 --> 00:11:26,370 +تكون water تفكرين كلمة water activity؟كمان مرة + +150 +00:11:26,370 --> 00:11:31,650 +water activity بتعبر عن مين؟ عن ال free water ال + +151 +00:11:31,650 --> 00:11:35,290 +requirement للميكروبات تختلف وبالتالي عندما يطلب + +152 +00:11:35,290 --> 00:11:39,150 +مني انا ان اتعامل مع ميكروب انا ما بعرفه، وش دي؟ + +153 +00:11:39,150 --> 00:11:42,530 +جالي لبنان حاضر له وصد؟ وصد، من الأشياء اللي لازم + +154 +00:11:42,530 --> 00:11:45,810 +ادرسها عن هذا الميكروب هو ال water activity + +155 +00:11:45,810 --> 00:11:50,130 +requirement كده بده water activity؟أو على الأقل + +156 +00:11:50,130 --> 00:11:55,050 +أعرف المعلومة هذه عشان أنا ممكن أفكر بطريقة تانية، + +157 +00:11:55,050 --> 00:11:58,470 +أقول هذا الميكروبط أنا بتعزله من بيئة، البيئة + +158 +00:11:58,470 --> 00:12:03,050 +بالمناسبة انتوا رايين اليوم دخلت القاعة من المبنى + +159 +00:12:03,050 --> 00:12:07,320 +شميته رؤحت المطر الجديدة؟رياح دي المطر الجديدة دي + +160 +00:12:07,320 --> 00:12:11,640 +عبارة راحة Actinomycetes بكتيريا طبعا بتشموها فقط + +161 +00:12:11,640 --> 00:12:15,920 +في .. في أول موسم هي من .. من الميكروفات الرائعة + +162 +00:12:15,920 --> 00:12:19,420 +اللي بتنتجلنا كمية كبيرة من ال antibiotics و بتنتج + +163 +00:12:19,420 --> 00:12:22,300 +بعض الأحيان .. أو مش بعض الأحيان ال soil + +164 +00:12:22,300 --> 00:12:27,440 +actinomycetes بتنتج اللي هي ال aromatic substances + +165 +00:12:27,440 --> 00:12:33,080 +اللي احنا بنشتمها مع تفاعلها مع الوقم المطرفبس + +166 +00:12:33,080 --> 00:12:36,340 +حبينا نقولكوا إنه هذه الرائحة اللي .. اللي .. اللي + +167 +00:12:36,340 --> 00:12:39,460 +بتذكرنا ده أو .. أو ممكن نشتجلها لأنها بتمر على + +168 +00:12:39,460 --> 00:12:43,940 +سنة حتى نشتمها مرة تانية فهي رائحة ل .. ل .. + +169 +00:12:43,940 --> 00:12:48,120 +للنتجة عن البكتيريا water, carbon source, nitrogen + +170 +00:12:48,120 --> 00:12:51,140 +source, minerals, growth factors و ال growth + +171 +00:12:51,140 --> 00:12:55,900 +factors غالبا تطلق على عناصر البكتيريا غير قادرة + +172 +00:12:55,900 --> 00:12:59,180 +على أنها تصنعها وبالتالي بتحتاجها إن احنا نزود + +173 +00:12:59,180 --> 00:13:03,710 +الوسط فيهانرجح على ال water water activity كمان + +174 +00:13:03,710 --> 00:13:07,390 +مرة قلنا لكوا مطلوب نعرف عن ال microbe ال water + +175 +00:13:07,390 --> 00:13:13,950 +activity بدي احزل microbe X من التربة وكلمة تربة + +176 +00:13:13,950 --> 00:13:17,690 +معناها حاجة ان في منافسين كثر مع هذا ال microbe + +177 +00:13:19,420 --> 00:13:23,320 +فإذا علمت إن ال requirement تبع هذا ال microbe + +178 +00:13:23,320 --> 00:13:28,020 +unique، كلمة unique يعني فريدة، مميزة، أستطيع أن + +179 +00:13:28,020 --> 00:13:32,560 +أتحكم في الوسط، تمام؟، بحيث أن يلائم هذا ال + +180 +00:13:32,560 --> 00:13:35,840 +microbe ولا يلائم ميكروبيرات أخرى، مصطلح بنسمي + +181 +00:13:35,840 --> 00:13:41,000 +selective isolation، عزل انتقائي أو عزل اختياري + +182 +00:13:42,770 --> 00:13:47,210 +selective isolation وفي المختبر بنعمل تقنية اسمها + +183 +00:13:47,210 --> 00:13:51,490 +selective media أو بصدرية انتقائية هنتكلم عنها في + +184 +00:13:51,490 --> 00:13:56,870 +وقت اللحظة ان شاء الله طيب انتوا الآن لما انا بقول + +185 +00:13:56,870 --> 00:14:06,070 +carbon source ايش بختاروا على بالكم؟ اللوحة + +186 +00:14:06,070 --> 00:14:11,880 +السابقة اللوحة السابقةإن ال carbon موجود في ال + +187 +00:14:11,880 --> 00:14:15,680 +carbohydrate في ال proteins وبالتالي الآن .. الآن + +188 +00:14:15,680 --> 00:14:18,820 +إذا بدي carbon source بدي أعرف برضه حاجة البكتيريا + +189 +00:14:18,820 --> 00:14:24,360 +هل بيلزمها الجلوكوز فقط؟ هل بيلزمها starch؟ ال + +190 +00:14:24,360 --> 00:14:28,000 +starch أرخص من الجلوكوز وبتنموا ع التنين بحطلها + +191 +00:14:28,000 --> 00:14:33,520 +starch يبقى بدّا قيلها carbon source وغالبا one + +192 +00:14:33,520 --> 00:14:38,610 +single carbon source ملائم يكفي البكتيريا تلميهone + +193 +00:14:38,610 --> 00:14:41,990 +single carbon source يعني بحطولها جليكوز بحطولها + +194 +00:14:41,990 --> 00:14:45,810 +لكتوزة حسب نوعية البكتيريا ال nitrogen source برضه + +195 +00:14:45,810 --> 00:14:50,330 +لازم مصادره ال proteins digested amino acids افضل + +196 +00:14:50,330 --> 00:14:53,670 +digested proteins مكسرة ال amino acids نيتروجينس + +197 +00:14:53,670 --> 00:14:57,390 +بيس نوكليك اسد ممشي الحال خدوا بالك انا انا بتكلم + +198 +00:14:57,390 --> 00:15:03,150 +عن nitrogen sources من مصادر natural يعني organic + +199 +00:15:03,150 --> 00:15:07,070 +افضل ففي اني كمان مصادر اخرى inorganic زي ال + +200 +00:15:07,070 --> 00:15:15,500 +ammoniaالأمون يعيش رمزها in H4 البترات + +201 +00:15:15,500 --> 00:15:18,500 +ال O3 مظبوطة ولا لأ؟ ممكن برضه نضيفها، ففي عندي + +202 +00:15:18,500 --> 00:15:23,640 +مصدر نيتروجيني من organic sources و من inorganic + +203 +00:15:23,640 --> 00:15:27,400 +sources، جالبا نحتاج أن نعمل mixture، يعني بنحط + +204 +00:15:27,400 --> 00:15:31,260 +organic مع inorganic، بس ال carbon source كم واحد؟ + +205 +00:15:32,090 --> 00:15:36,270 +carbon sources one carbon source ال minerals قلنا + +206 +00:15:36,270 --> 00:15:40,090 +هي عبارة عن تاكرين لما اتكلمنا عن مجموعة من + +207 +00:15:40,090 --> 00:15:44,450 +الكالسيوم و الفوسفرات هاد كلها عناصر تضعف بكميات + +208 +00:15:44,450 --> 00:15:48,330 +قليلة لإن البكتيريا بتكون بحاجتها بالفعل بكميات + +209 +00:15:48,330 --> 00:15:53,510 +قليلة و أعطيكم مثال بسيط على إنها لازم تكون بكميات + +210 +00:15:53,510 --> 00:15:56,250 +قليلة تعرفوا نحاس copper + +211 +00:15:59,750 --> 00:16:06,110 +essential لبعض ال enzymes في الخلية البكتيرية لكن + +212 +00:16:06,110 --> 00:16:10,690 +ال cover نفسه النحاس لو زادت كميته بيقتل البكتيريا + +213 +00:16:10,690 --> 00:16:15,330 +و اتعرف ان في قطعة معدنية مصنوعة من النحاس مصنوعة + +214 +00:16:15,330 --> 00:16:19,290 +من النحاس لو أخدنا قطعة معدنية مصنوعة من النحاس و + +215 +00:16:19,290 --> 00:16:22,590 +حطيناها على طبق فيه بكتيريا هلاجى حول البكتيريا + +216 +00:16:22,590 --> 00:16:24,950 +منطقة خالية من مادة البكتيريا + +217 +00:16:27,710 --> 00:16:32,070 +لكن required in trace amount بكميات ضئيلة جدا + +218 +00:16:32,070 --> 00:16:36,810 +النقطة الخاصة اللي قلنا عنها ال growth factors + +219 +00:16:36,810 --> 00:16:41,050 +that a cell must have for the growth but they + +220 +00:16:41,050 --> 00:16:46,590 +cannot synthesize by themselves بمعنى أنه لازم + +221 +00:16:46,590 --> 00:16:52,490 +أزود البكتيريا بهذه المكونات لأنها ما بتقدر تصنعها + +222 +00:16:52,490 --> 00:16:53,430 +بذلك + +223 +00:16:55,920 --> 00:16:59,500 +من ال growth factor بدنا ناخد معلومة باللون الأحمر + +224 +00:16:59,500 --> 00:17:05,400 +معمولة على اللوحة اسمها fastidious bacteria تركزوا + +225 +00:17:05,400 --> 00:17:08,400 +معايا في الجزئيها دي لإنه مهم جدا ال fastidious + +226 +00:17:08,400 --> 00:17:13,520 +bacteria هي بكتيريا تختلف + +227 +00:17:13,520 --> 00:17:16,060 +عن باقي أنواع البكتيريا او بتعطي مثال انا حاط هنا + +228 +00:17:16,060 --> 00:17:21,360 +على اللوحة بكتيريا اسمها hemophilus influenza + +229 +00:17:23,430 --> 00:17:27,710 +الاسم فقط لانه ظن في يوم من الأيام ان هي المسبب لل + +230 +00:17:27,710 --> 00:17:31,370 +influenza لانها عُزِلت من ألف الناس اللي كان عندهم + +231 +00:17:31,370 --> 00:17:34,790 +influenza وما كان الفيروس معروف في ذلك الوقت فأطلق + +232 +00:17:34,790 --> 00:17:37,910 +عليها Hemophilus influenza على ان هي المسبب لمين + +233 +00:17:37,910 --> 00:17:41,070 +لمرض ال influenza وبعد ذلك اكتشفت ان هو طبعا ال + +234 +00:17:41,070 --> 00:17:46,820 +virus المشهورالان هذه البكتيريا بتحتاج الى .. + +235 +00:17:46,820 --> 00:17:51,680 +مابتقدرش تعيش بدون two factors واحد بيسموه X واحد + +236 +00:17:51,680 --> 00:17:57,200 +بيسموه V factor وال X هو همين وال V factor هو + +237 +00:17:57,200 --> 00:18:04,320 +الناد فاكتور يبقى في عندي two compounds لازم أحطهم + +238 +00:18:04,320 --> 00:18:09,080 +في الوسط عشان البكتيريا هذه تقدر تم .. مابتقدرش + +239 +00:18:09,080 --> 00:18:13,160 +صنعهم بداليهامع العلم ان هدول الاتنين other + +240 +00:18:13,160 --> 00:18:18,720 +بكتيريا بتقدر تصنعهم other بكتيريا عادية بتصنعهم + +241 +00:18:18,720 --> 00:18:23,980 +فال بكتيريا اللى بتتميز بإنه إلها حاجة واضحة من ال + +242 +00:18:23,980 --> 00:18:28,380 +growth factors بنسميها fastelias البعض بيطلق عليها + +243 +00:18:28,380 --> 00:18:31,920 +البكتيريا المدلة من ناحية الغذائية يعني لها + +244 +00:18:31,920 --> 00:18:37,350 +requirement خاصةمن ال growth factors special amino + +245 +00:18:37,350 --> 00:18:40,330 +acids معينة بنضيفهم بعض ال purines و بعض ال + +246 +00:18:40,330 --> 00:18:43,930 +pyrimidines و بعض الأحيان ال vitamins فى أن ال + +247 +00:18:43,930 --> 00:18:50,330 +prosela بتحتاج vitamin B لبروسيلا وبالتالي بنسميها + +248 +00:18:50,330 --> 00:18:55,450 +برضه fastidious و غالبا ال fastidious بتكون slow + +249 +00:18:55,450 --> 00:19:00,500 +growing ماهي slow growing؟يعني بدل ما تاخد 24 ساعة + +250 +00:19:00,500 --> 00:19:04,520 +لمستعمرات حتى تظهر، خد بالك، هنتكلم الآن عن + +251 +00:19:04,520 --> 00:19:09,340 +العوامل اللي بتأثر على النمو، صح؟ وبالتالي يعني + +252 +00:19:09,340 --> 00:19:14,280 +بعتقد انه في نهاية الموضوع مضطرين نتكلم عن نمو + +253 +00:19:14,280 --> 00:19:17,200 +البكيريالأن احنا بتتكلم عن العوامل اللي بتأثر على + +254 +00:19:17,200 --> 00:19:20,100 +النمو التالي، احنا بتتكلم عن نمو itself، احنا + +255 +00:19:20,100 --> 00:19:25,340 +بننمو بالطول، بنجيس طولنا، ممكن نجيس الوزن تبعنا، + +256 +00:19:25,340 --> 00:19:28,300 +طبعا إن احنا زدنا في الوزن، بس نمو البكتيدا لا + +257 +00:19:28,300 --> 00:19:34,100 +يقاس بأي من المقاييس هذه، يقاس بالعددنمو البكتيريا + +258 +00:19:34,100 --> 00:19:43,480 +يقاس بالعدد 2 4 8 16 32 64 132 وها كده اللي هي + +259 +00:19:43,480 --> 00:19:47,580 +اتضاعف لإن الخلية بال C تنتهي تنتهي بال Zero أربعة + +260 +00:19:47,580 --> 00:19:52,520 +لإنه إنكسام فنائي بصير تبقى بتعبد الأرقام 2 4 8 16 + +261 +00:19:52,520 --> 00:19:56,840 +كده تحفظوهم في كل شيء كمان مرة اشتغلش مصطلح اللي + +262 +00:19:56,840 --> 00:20:00,970 +باللون الأحمر fastidias ماذا يعني؟يعني أن + +263 +00:20:00,970 --> 00:20:03,990 +البكتيريا لها requirement خاصة من ال growth + +264 +00:20:03,990 --> 00:20:08,710 +factors نجي لعامل تاني غير ال nutrient اللي هو + +265 +00:20:08,710 --> 00:20:13,660 +متعلق بال temperatureأكيد في التلاجة احنا بنحفظ + +266 +00:20:13,660 --> 00:20:19,300 +الأغنية ومع ذلك اكتر طلعنا من التلاجة أغنية صار + +267 +00:20:19,300 --> 00:20:24,200 +فيها عفن او فيها فساد زي حليب طلعت إله رائحة كريهة + +268 +00:20:24,200 --> 00:20:28,680 +أو شفنا بُقع على فاكة أو خضروات في التلاجة هذا + +269 +00:20:28,680 --> 00:20:33,180 +مؤشر على إيش؟ على إن المسبب يستطيع إنه في درجات + +270 +00:20:33,180 --> 00:20:36,240 +حرارة منخفضة لدرجات حرارة التلاجة هذا قبل حتى + +271 +00:20:36,240 --> 00:20:39,120 +موضوع قطع الكهربا، هذا عمر طبيعي إنه يصير في فساد + +272 +00:20:39,120 --> 00:20:39,720 +في التلاجة + +273 +00:20:42,440 --> 00:20:46,920 +ننتقل لموضوع تاني غير تلاجة، هناك بعض الأطعمة تحفظ + +274 +00:20:46,920 --> 00:20:53,740 +ساخنة، كيف تحفظ ساخنة؟ يعني مثلا عندنا اليوم وليمة + +275 +00:20:53,740 --> 00:21:00,180 +و حضرنا الطعام الساعة أحداشر اتناشر كان جاهز، لكن + +276 +00:21:00,180 --> 00:21:04,570 +الوليمة الساعة خمسة المساءوبالتالي انا مضطر احفظ + +277 +00:21:04,570 --> 00:21:08,370 +الغذاء هذا في درجة حرارة خمسين او ستين درجة مقوية + +278 +00:21:08,370 --> 00:21:13,010 +عشان يلاله ساخن مايتسخنش برة اخرى لانه بيفقد بعض + +279 +00:21:13,010 --> 00:21:16,950 +ال quality السابقة اللي هو تتسخن و برد هناك بعض + +280 +00:21:16,950 --> 00:21:20,110 +البكتيريا قد تنمو في هذا الغذاء و هتسبب food + +281 +00:21:20,110 --> 00:21:25,310 +poisoningتمام؟ لإن جنافيان دى فى التلاجة مش خالصان + +282 +00:21:25,310 --> 00:21:28,570 +وعلى حرارة مرتفعة برضه ايه؟ فى أننا ناس او فينا + +283 +00:21:28,570 --> 00:21:31,690 +مجموعات من البكتيريا عندها المقضة عليها أنها تنمو + +284 +00:21:31,690 --> 00:21:34,670 +و تماننا نزيزة طب احنا اجسامنا سبعة و تلاتين درجة + +285 +00:21:34,670 --> 00:21:38,210 +موية اللى هى عالى جدا وليها مخفضة وبرضه بنصاد + +286 +00:21:38,210 --> 00:21:42,960 +الاتهابات للبكتيريا، إذن حاجة البكتيرياللحرارة + +287 +00:21:42,960 --> 00:21:46,780 +تختلف من نوع الباتيريا صنفناه من تصنيف بسيط في + +288 +00:21:46,780 --> 00:21:50,220 +تصنيفات أعقد من هذه إلى ثالث أصناف Cyclophilic محب + +289 +00:21:50,220 --> 00:21:54,360 +للبرودة و Mesophilic محب لدرجة حرارة الوسطى و + +290 +00:21:54,360 --> 00:22:00,580 +Thermophilic المحب للهوايا المرتفعة 15-20 طبعا + +291 +00:22:00,580 --> 00:22:03,280 +خدوا بالكم انا هتكلم عن المصطلح الآن او تلت + +292 +00:22:03,280 --> 00:22:07,320 +مصطلحها احفظوهم كويس او فهموهم كويس الهندية حاسمة + +293 +00:22:07,320 --> 00:22:11,080 +minimum و حاسمة maximum و حاسمة optimum + +294 +00:22:28,860 --> 00:22:37,590 +أفضل نمو هو كلمة optimumالان واضح انه لما انا عملت + +295 +00:22:37,590 --> 00:22:41,030 +إشارة بالأصابيع قلت minimum و maximum و في النص + +296 +00:22:41,030 --> 00:22:48,530 +ايه ال optimum لا يعني ان الاصبع والأوسط في النص + +297 +00:22:48,530 --> 00:22:51,730 +ان درجة الحرارة ال optimum بتكون بين التنتين قد + +298 +00:22:51,730 --> 00:22:55,450 +تكون قريبة لل minimum او قد تكون قريبة ال maximum + +299 +00:22:55,450 --> 00:23:00,110 +او قد تكون في المنتصف يعني ليس هنا قانون بيقول ان + +300 +00:23:00,110 --> 00:23:04,380 +ال optimum وين يكون موجودقريب من ال .. و هنشوف + +301 +00:23:04,380 --> 00:23:07,880 +كمان صورة الآن و نريكوا أن ال optimum من الاشريشيا + +302 +00:23:07,880 --> 00:23:13,000 +كولاي قريب من ال maximum يعني مافيش يقولنا قانون + +303 +00:23:13,000 --> 00:23:17,900 +بيحكم، بيعتمد على نوع البكي اتفقنا كام صنف على حسب + +304 +00:23:17,900 --> 00:23:23,240 +الحرارة؟ cyclophilic, mesophilic, thermophilic و + +305 +00:23:23,240 --> 00:23:26,140 +أنا بقولكوا الكتب مليئة بأرقام مختلفة عن الأرقام + +306 +00:23:26,140 --> 00:23:31,030 +اللي موجودة هنافخلينا نتظن ب one number او رقم + +307 +00:23:31,030 --> 00:23:35,770 +المولود أمامنا الان ال 15-20 مش minimum و maximum + +308 +00:23:35,770 --> 00:23:42,290 +الصحيح ال optimum تبعهم من 15-20 لكن قد يكون هناك + +309 +00:23:42,290 --> 00:23:44,950 +growth على أربع درجات مقوية التلاجة بتكون من 2 ل8 + +310 +00:23:44,950 --> 00:23:45,510 +يا تلاجة + +311 +00:24:05,870 --> 00:24:09,230 +القائمة اللي انتم مش شايفينها وانا بالكاد استطيع + +312 +00:24:09,230 --> 00:24:14,760 +ان اقراهاهيبقى عن مجموعة من أسماء البكتيريا بس بدي + +313 +00:24:14,760 --> 00:24:19,560 +أوريكوا أمثلة Listeria monocytogenes ال minimum + +314 +00:24:19,560 --> 00:24:24,860 +تبعها one يعني تنمو هنا دلوقت بالتلاجة for sure صح + +315 +00:24:24,860 --> 00:24:31,420 +ال maximum خمسة واربعين ال optimum يتراوح من + +316 +00:24:31,420 --> 00:24:37,080 +تلاتين لسبعة و تلاتين قريب لل maximum نعمفى هن دى + +317 +00:24:37,080 --> 00:24:41,060 +ال vibrio marinus و marinus واضح من اسمها بحرية + +318 +00:24:41,060 --> 00:24:46,600 +اربعة برضه بتنمو فى تلاجة بتنمو مش فى تلاجة ال + +319 +00:24:46,600 --> 00:24:52,880 +optimum ممكن تدهش طب احنا جولنا ال minimum اربعة + +320 +00:24:52,880 --> 00:24:55,920 +هل يعني ذلك انه + +321 +00:25:00,100 --> 00:25:02,800 +عشان تلاتة اتنين واحدش هتنمو، ولكن اربعة، خمسة، + +322 +00:25:02,800 --> 00:25:06,980 +ستة، واتذكر تلاتة يقولنا من اتنين لتمانية، وبتالي + +323 +00:25:06,980 --> 00:25:12,000 +فيه إمكانيلا النموم، صحيح مش النمو optimum عشان + +324 +00:25:12,000 --> 00:25:14,900 +هيك اللي بنقول احنا ال low temperature و ال slow + +325 +00:25:14,900 --> 00:25:18,960 +down، عشان ال slow down هتبطئ، بدل الطعام مايفسد + +326 +00:25:18,960 --> 00:25:23,460 +خلال يومين لو كان ع سبعة و تانية درجة مقوية، بيفسد + +327 +00:25:23,460 --> 00:25:25,360 +مثلا بعد أسبوع أو أسبوعين + +328 +00:25:31,510 --> 00:25:34,450 +الجدل اللى قدامنا كمان مرة بوضح لي الميكروبات ال + +329 +00:25:34,450 --> 00:25:36,610 +minimum و ال maximum طبعا مش هنحفظهم هدول + +330 +00:25:36,610 --> 00:25:41,830 +الموجودين في أمامنا على اللوان لكن الشكل اللى + +331 +00:25:41,830 --> 00:25:45,330 +قدامكوا هذا شكل كتير كويس بيفهمنا الفكرة اللى بدأت + +332 +00:25:45,330 --> 00:25:48,950 +فيها أنا ليها ال minimum و ال maximum هي طبعا + +333 +00:25:48,950 --> 00:25:56,190 +درجات الحرارة ال degrees Celsius عندي حوالى 9 او 8 + +334 +00:25:56,190 --> 00:25:59,850 +درجات مئوية below which Escherichia coli will not + +335 +00:25:59,850 --> 00:26:06,620 +growأقل من 8 الإشريشيا كولاي مش هتنمو، أكتر من 48 + +336 +00:26:06,620 --> 00:26:11,340 +درجة مئوية لن تنمو البكتيريا، لن تنمو الإشريشيا + +337 +00:26:11,340 --> 00:26:18,160 +كولاي، وين أفضل نمو؟ على 37، 37 كنا كم مرة أقرب + +338 +00:26:18,160 --> 00:26:22,180 +إلى 48 من مين؟ من اللي هو ال minimum، يعني أقرب + +339 +00:26:22,180 --> 00:26:27,710 +إلى ال maximum من ال minimumوفي هذه النقطة إشارة + +340 +00:26:27,710 --> 00:26:32,930 +واضحة بإنه ال .. ال .. ال .. ال .. ال .. + +341 +00:26:32,930 --> 00:26:37,010 +الmicrobial تختلف في حالتها لل temperature + +342 +00:26:37,010 --> 00:26:40,250 +وبالتالي بدنا نكون عارفين إن معظم ال medically + +343 +00:26:40,250 --> 00:26:43,130 +important bacteria .. medically important bacteria + +344 +00:26:43,130 --> 00:26:47,750 +can grow at a mesophilic temperature لإنهم بسببنا + +345 +00:26:47,750 --> 00:26:51,970 +أمراض وبالتالي جسمنا 37 و ال optimum بتبعهم بيكون + +346 +00:26:51,970 --> 00:26:58,010 +قريب من هذه الدرجةميجي لل pH ال hydronium ion + +347 +00:26:58,010 --> 00:27:02,110 +concentration او ال pH زي ما ال temperature عامل + +348 +00:27:02,110 --> 00:27:08,310 +حاسم في نمو البكتيريا ايضا هاد اللي عامل حاسم ايضا + +349 +00:27:08,310 --> 00:27:13,450 +وقاله minimum وقاله maximum وقاله optimum عارفين + +350 +00:27:13,450 --> 00:27:17,430 +ليش؟ مين فيكوا درس clinical chemistry؟ + +351 +00:27:19,470 --> 00:27:23,930 +مين عمل فحص ال glucose؟ او حد ما وصلوش للفحوصات + +352 +00:27:23,930 --> 00:27:30,230 +هذه في المختبأ؟ طيب الإنزيمات .. الإنزيمات احنا + +353 +00:27:30,230 --> 00:27:36,000 +بنعملها فحص على pH محددهذا ال pH هو ال optimum + +354 +00:27:36,000 --> 00:27:39,220 +معناه الحاجة ان في عندها مرضوية minimum و في عندها + +355 +00:27:39,220 --> 00:27:44,540 +maximum below which لذلك لما احنا بنقول ان ال pH + +356 +00:27:44,540 --> 00:27:48,620 +غير ملائم هو غالبا غير ملائم لمين ده الإنزيمات + +357 +00:27:48,620 --> 00:27:54,760 +اللي موجودة في الخليل هناك بعض أنواع من الميكروبات + +358 +00:27:54,760 --> 00:28:00,410 +بتحب ال pH المتعددو في ميكروبات بتحب ال pH المنخفض + +359 +00:28:00,410 --> 00:28:03,810 +كلكوا بتعرفوها و بتاكلوها كل يوم بتاكلوها او مش كل + +360 +00:28:03,810 --> 00:28:09,630 +يوم البعض منكوا المخللات المخلل المخلل أصلا تصنيعه + +361 +00:28:09,630 --> 00:28:15,070 +هو عبارة عن بكتيريا بتخفض ال pH تبع الخضروات اللي + +362 +00:28:15,070 --> 00:28:17,790 +بتعملوها المخلل زي الأخيار على سبيل المثال بنزل ال + +363 +00:28:17,790 --> 00:28:24,390 +pH تبعه إلى طعم أو إلى مضاق المقبول لديكم الحليب + +364 +00:28:24,390 --> 00:28:30,830 +بتحول للبناللبن بيكون حامض، حموضة مقبولة لديكم، + +365 +00:28:30,830 --> 00:28:35,410 +لما بنزينش بيسموه حمضمحمد مش معناته الحاجة انه .. + +366 +00:28:35,410 --> 00:28:40,010 +انه فاسد، محمد معناته انه درجة الحموضة نزلت .. + +367 +00:28:40,010 --> 00:28:43,790 +يعني .. يعني ارتفعت الحموضة من ال beach نزل تمام + +368 +00:28:43,790 --> 00:28:48,190 +إلى درجة غير مقبولة ذوقيا، اللي هو الذوق، فلذلك هو + +369 +00:28:48,190 --> 00:28:52,490 +ليس فساد صحي وانما فساد ذوقي، بنسميه organoleptic + +370 +00:28:52,490 --> 00:28:55,650 +spoilage organoleptic spoilage يعني ان النفس لا + +371 +00:28:55,650 --> 00:29:01,810 +تتدوق بيه إيجابية، تتدوق بيه سالمةإذا نعرف أنه في + +372 +00:29:01,810 --> 00:29:06,250 +أسيلوفيليك محب للحق لل .. لل pH المقفض في نموذج + +373 +00:29:06,250 --> 00:29:11,510 +صارخ طبي لبكتيريا بتحب ال alkaline pH اللي هي ال + +374 +00:29:11,510 --> 00:29:16,630 +Vibrio و دبالكوا احنا المعلومات اللي بتدرسها في ال + +375 +00:29:16,630 --> 00:29:21,550 +microbiology من باب اعرف عدوك اعرف عدوك لماذا + +376 +00:29:21,550 --> 00:29:26,890 +نتعلم عن أعدائنا او عن أعدائنا نتعلم عشان احنا + +377 +00:29:26,890 --> 00:29:31,190 +نعرف كيف نتعافل معهمنتعامل معهم سواء كان بإيجارية + +378 +00:29:31,190 --> 00:29:35,870 +أو بسلبية، في الآخر نتعامل معهم البكتيريا اللي من + +379 +00:29:35,870 --> 00:29:40,810 +نوع Vibrio بتحب ال alkaline pH احنا مسكنا هذه + +380 +00:29:40,810 --> 00:29:46,290 +المعلومة، تمام؟ واستفدنا منها في أكثر من طريقة، + +381 +00:29:46,290 --> 00:29:52,070 +ناخد الاستفادة الأولى لما أردنا عزل البكتيريا من + +382 +00:29:52,070 --> 00:29:56,930 +ال stoolيحتوي لجرام الواحد على عشر أقص اتناش + +383 +00:29:56,930 --> 00:30:01,830 +تقريبا بكتيريا لكل جرام و من أنواع مختلفة و بدي + +384 +00:30:01,830 --> 00:30:04,310 +أعزل مايكروب واحد اسمه Vibrio من بين كل + +385 +00:30:04,310 --> 00:30:08,490 +هالمايكروبات المهولة فبرا بحطهم في وسط ال بي اتش + +386 +00:30:08,490 --> 00:30:13,150 +سبعه alkaline كلهم بموتوا إلا ال Vibrio يبقى + +387 +00:30:13,150 --> 00:30:16,350 +استفدت من ال .. استفدت من المعلومة بال selective + +388 +00:30:16,350 --> 00:30:21,680 +isolation معي ولا مش معي؟استفادت معلومة تانية من + +389 +00:30:21,680 --> 00:30:25,060 +ناحية طبية، أنا مش جادر أغلى الميه في .. ماعنديش + +390 +00:30:25,060 --> 00:30:28,140 +طاقة أغلى الميه للناس و أقول للناس اغلىوا الميه، + +391 +00:30:28,140 --> 00:30:31,160 +بنقولهم بطريقة تانية، بنقولهم ممكن تعصروا لمون على + +392 +00:30:31,160 --> 00:30:37,240 +كأس الماء قبل شربه، اللامونش بيعمل، بيخفض ال pH و + +393 +00:30:37,240 --> 00:30:43,510 +بيقتل ال .. ال vibrio كلها، بيجعل ظروفها صعبةالقصد + +394 +00:30:43,510 --> 00:30:48,450 +من الكلام انه لما بنتعلم المعلومة احنا المعلومة مش + +395 +00:30:48,450 --> 00:30:52,850 +فقط للحفظ وانما كيف يمكن استغلال هذه المعلومة + +396 +00:30:52,850 --> 00:30:57,130 +تطبيقيا وانا بجيب أمثلة خلال الشرح لكن انا اتمنى + +397 +00:30:57,130 --> 00:31:01,130 +ان العقلية تبعتنا العقلية تبعت الطالب تتحول من + +398 +00:31:01,130 --> 00:31:06,920 +متلقي معلوماتالى قدرة تحليلية انا كيف ممكن استفيد + +399 +00:31:06,920 --> 00:31:10,360 +من هذه المعلومة وانا اعتقد ان مجال ال microbiology + +400 +00:31:10,360 --> 00:31:14,480 +من اروع العلوم اللى ممكن الطالب او اللى دانس لي + +401 +00:31:14,480 --> 00:31:19,220 +هذا المصاد انه يستفيد من المعلومات بشكل تطبيقى و + +402 +00:31:19,220 --> 00:31:24,100 +ايجابى معظم الميكروبات اللى بسببنا امراض هى من + +403 +00:31:24,100 --> 00:31:28,300 +النوع الاول اللى بتحب ال ph تبع ال near ال + +404 +00:31:28,300 --> 00:31:34,560 +neutrality القريب من اللى هو التعاملبرضه جدول اخر + +405 +00:31:34,560 --> 00:31:40,000 +بوضح لنا بعض الميكروبات شوفوا مثلا الميكروب الاول + +406 +00:31:40,000 --> 00:31:43,820 +اسمه thiobacillus thioxidans ال minimum pH point + +407 +00:31:43,820 --> 00:31:49,720 +five يعني very very very acidic و ال maximum اربعة + +408 +00:31:49,720 --> 00:31:53,940 +لستة و ال optimum من اتنين لاثنين و تمانية من عشر + +409 +00:31:53,940 --> 00:31:59,760 +في Vibrio عندنا ولا مش Vibrio؟في اندي هنا واحدة من + +410 +00:31:59,760 --> 00:32:07,080 +المحبات لل pH ال minimum pH تبعها خمسة الى خمس + +411 +00:32:07,080 --> 00:32:12,060 +تمانية ال optimum قريب من ال neutral ال maximum من + +412 +00:32:12,060 --> 00:32:17,030 +تمانية و نص الى تسعةفي عندي لورع على طول Erwinia + +413 +00:32:17,030 --> 00:32:20,750 +من تسعة و تلاتة، ال maximum، في عندي كمان + +414 +00:32:20,750 --> 00:32:25,290 +Thiobacillus تسعة، يعني في عندي تنوع أفوان، تنوع + +415 +00:32:25,290 --> 00:32:29,930 +مختلف من الميكروبات، البعض منها .. البعض منها + +416 +00:32:29,930 --> 00:32:34,190 +بيكون مفضل ال .. ال .. ال acidic، البعض الأخر يفضل + +417 +00:32:34,190 --> 00:32:37,690 +ال .. ال alkaline، لكن كما ذكرتوا لكم معظم ال + +418 +00:32:37,690 --> 00:32:40,770 +medical important bacteria they prefer ash اللي هو + +419 +00:32:40,770 --> 00:32:48,730 +pH near and neutralنجي للعامل الثالث بعد الرابع + +420 +00:32:48,730 --> 00:32:51,790 +اعتقد هذا هو الاول كان ال nutrient التاني كان + +421 +00:32:51,790 --> 00:32:53,890 +الحرارة التالت كان البيوتش الان هو ال oxygen + +422 +00:32:53,890 --> 00:32:58,870 +required لو افترضنا افترضنا جدلا اي واحد و جالكه + +423 +00:32:58,870 --> 00:33:05,120 +ال oxygen سام للبشرطبعا حتى هجوموا على طول انتوا و + +424 +00:33:05,120 --> 00:33:10,160 +تقولوا انه انت تتكلم كلام غير منطقي البتة لإن احنا + +425 +00:33:10,160 --> 00:33:15,220 +إذا منعنا ال oxygen بنموت صح؟ فكيف لك أن تصف ال + +426 +00:33:15,220 --> 00:33:18,660 +oxygen بالمادة السامة للبشر؟ أنا الآن بتوضح حالي + +427 +00:33:18,660 --> 00:33:25,920 +الفكرة و أرجو انكوا تكونوا صابورين لفهم الموضوع ال + +428 +00:33:25,920 --> 00:33:30,880 +oxygen رمزه O2 صح؟ و O2 يعني ذرة و ذرة من ال + +429 +00:33:30,880 --> 00:33:35,300 +oxygen، ذرتين oxygenالوظيفة اللى بيعملها الاكسجين + +430 +00:33:35,300 --> 00:33:40,240 +فى داخل خلايانا احنا انه it acts as a final + +431 +00:33:40,240 --> 00:33:42,680 +electron acceptor ميش يعني final electron + +432 +00:33:42,680 --> 00:33:45,880 +acceptor؟ مستقبل أخي الالكترونات زوج من + +433 +00:33:45,880 --> 00:33:48,900 +الالكترونات بتطلع من مركب لمركب لمركب لمركب وفي + +434 +00:33:48,900 --> 00:33:52,740 +الآخر لازم واحد يحمله مين اللى بيحمله؟ الاكسجين + +435 +00:33:52,740 --> 00:33:58,800 +وإن هذه العملية عملية صعبة شوية قد تقسم ظهر + +436 +00:33:58,800 --> 00:34:06,490 +الاكسجينهي تشطر فيتحول الى O و O بنسميها O singlet + +437 +00:34:06,490 --> 00:34:12,350 +singlet واحدة طبيعة الأكسجين مابقدرش يعيش singlet + +438 +00:34:12,350 --> 00:34:19,490 +في بعض الإنزيمات في الخلية أطول بتشوف مش بعض + +439 +00:34:19,490 --> 00:34:23,910 +الإنزيمات حتى لحاله بدون إنزيمات ال O مع ال H2O + +440 +00:34:23,910 --> 00:34:28,470 +بيكونوله H2O2 hydrogen peroxide ال hydrogen + +441 +00:34:28,470 --> 00:34:30,110 +peroxide يوصف + +442 +00:34:34,160 --> 00:34:38,280 +Powerful Oxidizing agent يعني هيدمر بعض + +443 +00:34:38,280 --> 00:34:41,600 +الموليكيولز في الخلية زي ال DNA زي ال RNA زي ال + +444 +00:34:41,600 --> 00:34:43,460 +protein زي ال carbohydrate اللي هي بيسموها ال + +445 +00:34:43,460 --> 00:34:48,420 +macro molecules الجزيئات الكبيرة ال O التانية + +446 +00:34:48,420 --> 00:34:52,260 +وراها ال O التانية قد تتحول إلى ما يسمى super + +447 +00:34:52,260 --> 00:34:58,150 +oxideيبقى في اندي أو تفاعل مع هيدروجين يعفر مع ماء + +448 +00:34:58,150 --> 00:35:02,730 +وتكون H2O2 و واحدة تانية اتحولت إلى ما يسمى بال + +449 +00:35:02,730 --> 00:35:06,070 +super oxide و كلا هما ال hydrogen peroxide و ال + +450 +00:35:06,070 --> 00:35:09,250 +super oxide كلا هما very powerful oxidizing agent + +451 +00:35:09,250 --> 00:35:16,230 +بيقتل الخلايا إذا احنا ميتين احنا ميتين بالطريقة + +452 +00:35:16,230 --> 00:35:22,850 +هذه طب ليش احنا مش ميتين؟لأن الجسم بتاعنا في + +453 +00:35:22,850 --> 00:35:27,630 +system بيحمينا من ال oxygen toxicity كل ما يتكون + +454 +00:35:27,630 --> 00:35:32,370 +super oxide بيعارب تأثيره هذا ال انزين كل ما يتكون + +455 +00:35:32,370 --> 00:35:35,970 +hydrogen peroxide بيحلله هذا ال انزين هنشوف هذه + +456 +00:35:35,970 --> 00:35:42,650 +الانزيمات بعد قليل لكن بناناعرف أن ال oxygen سام + +457 +00:35:43,850 --> 00:35:47,570 +الوكسجين ساعة هذا المفهوم الجديد بالنسبالكوا ارجو + +458 +00:35:47,570 --> 00:35:52,170 +انكوا تكونوا افهمتوا ليش احنا وصلنا لهذا المفهوم + +459 +00:35:52,170 --> 00:35:55,070 +اللوحة اللي امامكوا ليس لها علاقة بالمفهوم اللي + +460 +00:35:55,070 --> 00:36:01,830 +حكيته لكن كويس الان نعرض اللوحة و نشوف الشكل + +461 +00:36:01,830 --> 00:36:06,970 +اللي امامكوا ايش بيعمل لو انا حطيت بكتيريا في + +462 +00:36:06,970 --> 00:36:12,460 +انبوبةبكتيريا رجهولة قد تتخذ واحد من الخمس أشكال + +463 +00:36:12,460 --> 00:36:17,740 +اللي أمامكم رقم واحد البكتيريا و النامية على الصدر + +464 +00:36:17,740 --> 00:36:23,240 +البكتيريا رقم اتنين و النامية بالقعب الأولى هوائية + +465 +00:36:23,240 --> 00:36:29,000 +إجباريةوالتانية لا هوائية إشبالية ليش أنا استنتجت + +466 +00:36:29,000 --> 00:36:33,000 +هذا الاستنتاج لأنه الأنبوبة بشكل عام أي عمود من + +467 +00:36:33,000 --> 00:36:36,420 +الماء لو جينا نطلع على مستوى ال oxygen فيه كل ما + +468 +00:36:36,420 --> 00:36:39,900 +نزلنا تحت كل ما قال مستوى ال oxygen كل ما طلعنا + +469 +00:36:39,900 --> 00:36:44,140 +فوق كل ما زاد مستوى ال oxygen ففوق بالفعل في رقم + +470 +00:36:44,140 --> 00:36:49,040 +واحد في oxygen نبت البكتيريا تحت فيش oxygen نبت + +471 +00:36:49,040 --> 00:36:52,920 +البكتيريا فهذه بكتيريا لا هوائيةلحظوا مانا ماتش + +472 +00:36:52,920 --> 00:36:57,460 +فوق لإنه فيه Oxygen إيه صار فيها؟ ماتت البكتيريا، + +473 +00:36:57,460 --> 00:37:01,980 +اللي فوق ماتت، ماتت لإن ال Oxygen سام بالنسبيلها، + +474 +00:37:01,980 --> 00:37:06,260 +زي ما احنا سام بالنسبيلنا، بس الفرق بيننا وبينها + +475 +00:37:06,260 --> 00:37:11,000 +إنه هي لاتمتلك ما نمتلك نحن من إنزيمات تعادل صمية + +476 +00:37:11,000 --> 00:37:15,480 +الواء فبتوقع البكتيريا الهوائية يكون نفس الأنظمة + +477 +00:37:15,480 --> 00:37:18,100 +اللي عندنا موجودة عندها، بينما البكتيريا اللي + +478 +00:37:18,100 --> 00:37:22,360 +هوائية ماعنداش هذه الأنظمةرقم تلاتة، والله + +479 +00:37:22,360 --> 00:37:24,820 +البكتيري هذا اللي فوق، التلاتة من ما هي فارقة + +480 +00:37:24,820 --> 00:37:29,400 +معاها صح؟ لأ مش فارقة معاها، نامية فوق و نامية + +481 +00:37:29,400 --> 00:37:37,060 +تحت، هتكلم عنها بعد قليل، رقم أربع، لأ و النامية، + +482 +00:37:37,060 --> 00:37:41,550 +على السطح ولا تحت السطح شوية؟هذه بكتيريا بتحب تشيل + +483 +00:37:41,550 --> 00:37:44,630 +أكسجين بكميات كبيرة، بتحب بكميات قليلة، بيسموها + +484 +00:37:44,630 --> 00:37:50,330 +micro-aerophilic يعني ترغب بكميات أكسجين + +485 +00:37:50,330 --> 00:37:53,990 +مُنقَفَرة، نسبة الأكسجين في الجو اللي جاتاش يا + +486 +00:37:53,990 --> 00:37:57,970 +فنات، يعني في الجو العادي، في البيئة اللي احنا + +487 +00:37:57,970 --> 00:38:02,110 +عايشين فيها، جاتاش نسبة الأكسجين؟ عشرين في المين، + +488 +00:38:02,110 --> 00:38:06,330 +تقريبًا عشرين تسعة من عشر، يعني تقريبًا واحد عشرين + +489 +00:38:06,330 --> 00:38:10,880 +بالميةالبكتيرى الهوائية العادية اللى في رقم واحد + +490 +00:38:10,880 --> 00:38:16,320 +بتعيش عندها المستوى بدون اي مشاكل رقم اربع فى + +491 +00:38:16,320 --> 00:38:19,800 +اللوحة اللى هى ال microaerophilic فقط بده خمسة + +492 +00:38:19,800 --> 00:38:24,040 +فالمية فلاحظوا الخمسة فالمية وانا حصلتهم مش على + +493 +00:38:24,040 --> 00:38:29,070 +السطح مباشرة من تحت السطحرقم خمسة و رقم تلاتة إيش + +494 +00:38:29,070 --> 00:38:31,870 +الفرق بينهم؟ مين يرفع أيه اللي بيقول إيش الفرق بين + +495 +00:38:31,870 --> 00:38:35,890 +التنان؟ تلاتة هي تلاتة ما هي هوائية اغتيارية لإنه + +496 +00:38:35,890 --> 00:38:39,330 +نموها في كدة يوم بس فوق كان تركيزها أكتر عن + +497 +00:38:39,330 --> 00:38:43,830 +الأكسوجين لكن هناك لا هوائية اغتيارية يعني إنه كان + +498 +00:38:43,830 --> 00:38:47,470 +تنانيه في كل النموبة بس يعني إنه نموها فاقش كتير + +499 +00:38:47,470 --> 00:38:52,610 +كيف لا هوائية اغتيارية يعني؟ بتعيش في ظروف مافيهاش + +500 +00:38:52,610 --> 00:38:55,490 +.. فيها أكسوجين و مافيهاش بس بتفضل اللي مافيهاش + +501 +00:38:55,490 --> 00:38:59,430 +اغتياريةرقم تلاتة رقم تلاتة facultative أنا + +502 +00:38:59,430 --> 00:39:02,030 +aerobic و كلامك دقيق مائة بالمائة facultative أنا + +503 +00:39:02,030 --> 00:39:06,610 +aerobic لإنه نمت فوق و نمت تحت لكن نموها في الأعلى + +504 +00:39:06,610 --> 00:39:11,610 +أكتر لإن أنا أفضل aerobic رقم خمسة لا يطلق عليها + +505 +00:39:11,610 --> 00:39:17,760 +اللقى المصطلح اللي أطلقتيه انت شايف ايه المصطلح؟لأ + +506 +00:39:17,760 --> 00:39:22,760 +يطلق عليها لهوائية تتحمل ولود الأكسجين بمعنى إنه + +507 +00:39:22,760 --> 00:39:29,640 +هي أصلا لهوائية لكن فوّت قالية لتحمل الأكسجين فهو + +508 +00:39:29,640 --> 00:39:33,840 +بالتالي نمت في كل الإنقوبة بنفس المستوى، خدوا + +509 +00:39:33,840 --> 00:39:38,360 +بالكوا، تحمل الشيء غير استخدام الشيء، يعني ما + +510 +00:39:38,360 --> 00:39:44,620 +استخدمت؟ الأكسجين، بينما رقم تلاتة فوجها ناشع عملت + +511 +00:39:45,920 --> 00:39:51,120 +استخدام ال oxygen، كمان مرة، خمس أنواع، انسوا + +512 +00:39:51,120 --> 00:39:54,800 +اللوحة، انسوا اللوحة، تعالوا نحفظهم على قاعد + +513 +00:39:54,800 --> 00:39:59,520 +الإصبع أو الأصابع، هوائية إجبارية، لا هوائية + +514 +00:39:59,520 --> 00:40:03,040 +إجبارية، في المنتصف facultative and European، ده + +515 +00:40:03,040 --> 00:40:08,570 +هي بحلقة وصل من الهوائية واللا هوائيةمش الأرهانة + +516 +00:40:08,570 --> 00:40:13,090 +هذه micro aerophilic هوائية لكن بدها كمية oxygen + +517 +00:40:13,090 --> 00:40:17,870 +قليلة الرابع اللي قريب من اللي لا هوائية بقولنا لا + +518 +00:40:17,870 --> 00:40:22,130 +هوائية وسميناها aerotolerant anaerobic يعني لا + +519 +00:40:22,130 --> 00:40:26,730 +هوائية ولكن تتحمل وجود ال oxygen فسنعمل خمسة ان + +520 +00:40:26,730 --> 00:40:31,750 +شاء الله التالتة + +521 +00:40:31,750 --> 00:40:37,830 +هي بكتيريا هوائية بكتيريا هوائيةماذا يعني تالتة + +522 +00:40:37,830 --> 00:40:41,370 +البكتيريا الهوائية؟ تستطيع أن تعيش في ورود الهواء + +523 +00:40:41,370 --> 00:40:45,870 +و عدم ورود الهواء تستغل الأكسجين و تستغل مراكبات + +524 +00:40:45,870 --> 00:40:50,750 +أخرى في التنفس، تمام؟ لكن خدوا بالكم من شغل، لو + +525 +00:40:50,750 --> 00:40:56,670 +أنا معاه جزئي جلوكوز و أريد أن أعمله oxidation أو + +526 +00:40:56,670 --> 00:41:00,170 +metabolism، متابوليزم دعوني أقول مصطلح أدق، لو + +527 +00:41:00,170 --> 00:41:04,870 +عملت له متابوليزم في ورود الأكسجين، سأحصل على 36 + +528 +00:41:04,870 --> 00:41:10,640 +ATPلو عملتله metabolism في غياب ال oxygen أحسنها + +529 +00:41:10,640 --> 00:41:17,260 +اتنين اتنين يبقى فعالية الطاقة الناتجة عن جزء + +530 +00:41:17,260 --> 00:41:20,920 +الجلوكوز في وجود ال oxygen بتكون ايه أفضل لذلك + +531 +00:41:20,920 --> 00:41:24,920 +الفاكولتات في أنا إيروف بتفضل النمو الهوائي عشان + +532 +00:41:24,920 --> 00:41:30,040 +انا لاجيناها فوق أعدادها أكبر من وين من تحت بينما + +533 +00:41:30,040 --> 00:41:33,560 +ال air tolerant bacteria قلنالكوامُمطَبَب النمو + +534 +00:41:33,560 --> 00:41:39,960 +فيها لإنه ليس الأكسجين دخل في النمو، ليس الأكسجين + +535 +00:41:39,960 --> 00:41:44,180 +أي دخل في النمو وإنما البكتيريا بطبيعتها لا + +536 +00:41:44,180 --> 00:41:48,220 +هوائية، لا تستخدم الأكسجين ولكن طورت قالية لحماية + +537 +00:41:48,220 --> 00:41:55,060 +نفسها من الأكسجين هذا اللوحة اللي أمامكم هي نفس + +538 +00:41:55,060 --> 00:42:01,000 +اللوحة اللي شفتوها بس بالكلام مش بالرسمأنا حكيت عن + +539 +00:42:01,000 --> 00:42:04,560 +الـ Oxygen toxicity concept اللي هي بتكلم عن ال + +540 +00:42:04,560 --> 00:42:09,420 +counter parts اللي هم ال systems الأنظمة اللي + +541 +00:42:09,420 --> 00:42:16,060 +استخدمت في التخلص من ال toxicity نتطلع على الدوحة + +542 +00:42:16,060 --> 00:42:23,790 +اللي قدامنا هنا أول معادلة بتقول 2O2 زي 2Hتو + +543 +00:42:23,790 --> 00:42:30,530 +باستخدام الانزيم اللي اسمه super oxide dismutase + +544 +00:42:30,530 --> 00:42:37,510 +SOD super oxide dismutase اتحولت الى H2O2 زائد O2 + +545 +00:42:37,510 --> 00:42:43,310 +لاحظوا شوية الـ2O2 ها دى اللى فوقيها شحطة هفير + +546 +00:42:43,310 --> 00:42:50,580 +شحطة ها دى صغيرة هذا المركب يسمى super oxideSuper + +547 +00:42:50,580 --> 00:42:55,100 +Oxide هذا الإشارة اللي عليه تسمى أو تعطي لقب Super + +548 +00:42:55,100 --> 00:43:00,240 +Oxide طيب ال Super Oxide Dismutase حول ال Super + +549 +00:43:00,240 --> 00:43:06,940 +Oxide إلى Hydrogen Peroxide من سم إلى سم، صح؟ هذه + +550 +00:43:06,940 --> 00:43:12,660 +خطوة على الطريقالخطوة التانية ال hydrogen peroxide + +551 +00:43:12,660 --> 00:43:16,460 +اللي اتكوّن سواء من ال super oxide بسم تايز او + +552 +00:43:16,460 --> 00:43:20,660 +اللي اتكوّن لحاله الاسلام دي hydrogen peroxide + +553 +00:43:20,660 --> 00:43:25,400 +انزيم الكتاليزا على طول بياخده و بيحوله إلى رزقنا + +554 +00:43:25,400 --> 00:43:30,940 +زائد أكسجين يبقى رجعنا الأكسجين زي ما كان تخلصنا + +555 +00:43:30,940 --> 00:43:34,560 +من مين الآن من السمية تبع هذا الأكسجين + +556 +00:43:37,090 --> 00:43:41,810 +طيب في عندي معادلة تالتة ايش دخلها المعادلة؟ و لو + +557 +00:43:41,810 --> 00:43:47,570 +التاني خلصه؟ التالتة ايش دخلها دي؟ خدوا بالكم هناك + +558 +00:43:47,570 --> 00:43:51,570 +بعض المخلوقات لا تحتوي على كتاليز تحتوي على حاجة + +559 +00:43:51,570 --> 00:43:56,130 +اسمها peroxidase ال peroxidase بيقوم بنفس العمل + +560 +00:43:56,130 --> 00:43:59,010 +اللي بيقوم بيها الكتاليز لكن بطريقة مختلفة بطريقة + +561 +00:43:59,010 --> 00:44:04,490 +مختلفة أعطيكم مثال هنا فلو أنا اشتري عندي هنا اتو + +562 +00:44:04,490 --> 00:44:12,220 +في المعادلةهنا ال H2O2 بيعطيها لمركّب لمركّب مختزل + +563 +00:44:12,220 --> 00:44:18,820 +مركّب مختزل بوجود البروكسدايز بطلع مي زي المركّب + +564 +00:44:18,820 --> 00:44:23,420 +مؤكسل رحمة + +565 +00:44:23,420 --> 00:44:27,280 +الهيدروجينراح منه الهيدروجين فصار لنا إيه؟ مؤكسد + +566 +00:44:27,280 --> 00:44:33,480 +لكن ما طلعش Oxygen بمعنى أخر المحصلة النهائية ال + +567 +00:44:33,480 --> 00:44:37,680 +hydrogen peroxide فى التانية اتحول لمين؟ لماء فى + +568 +00:44:37,680 --> 00:44:43,100 +الأولى اتحول لماء زاد Oxygen و فى التالى اتحول ماء + +569 +00:44:43,100 --> 00:44:48,960 +إلى مركب مؤكسد بجكال مختصر و اتأكسد فى الحالتين + +570 +00:44:48,960 --> 00:44:55,330 +خلصنا من صميم ال hydrogen peroxideطبعا هذا نقطة + +571 +00:44:55,330 --> 00:44:59,130 +يجب انه نجد في عينها دقيقة واحدة عشان نقولكوا إيش + +572 +00:44:59,130 --> 00:45:04,210 +ممكن أستفيد منها تتذكروا ال gram-bostev-cocci؟ + +573 +00:45:04,210 --> 00:45:08,450 +gram-bostev بكتيريا لما تكون البكتيريا شكلًا + +574 +00:45:08,450 --> 00:45:13,790 +كوكساي بنقول gram-bostev كوكساي كروي يعني من أشهر + +575 +00:45:13,790 --> 00:45:18,130 +الأسماء اللي هتعرفوها أو عرفتوها is staphylococcus + +576 +00:45:18,130 --> 00:45:22,940 +aureusو من أشهر الميكروفات اللي انتوا مرضتوا فيها + +577 +00:45:22,940 --> 00:45:27,860 +في يوم من الأيام باللواز، Pencilitis اسمها + +578 +00:45:27,860 --> 00:45:33,300 +streptococcus,adenostepilococcus و streptococcus + +579 +00:45:33,300 --> 00:45:37,880 +التنتين اجرام بوسط الكوكساية، أحد أهم الفحوصات + +580 +00:45:37,880 --> 00:45:43,770 +المميزة بين التنتين فحص الكتالينالستافيلوكوكس + +581 +00:45:43,770 --> 00:45:49,890 +كتاليز positive بتطلع كتاليز بينما الستريبتوكوكس + +582 +00:45:49,890 --> 00:45:55,190 +كتاليز negative تعاولوا كيف نعمل فحص لكي تكونوا + +583 +00:45:55,190 --> 00:46:00,030 +عارفين أن كل معلومة بندرسها هنا لها تطبيق في + +584 +00:46:00,030 --> 00:46:04,770 +المعمل هاي شريحة بنجيب قطرة من ال hydrogen + +585 +00:46:04,770 --> 00:46:10,250 +peroxide بنحط على القطرة البكتيرى اللي بنفحصها + +586 +00:46:11,160 --> 00:46:16,760 +القادة البكتيريا إذا فيها كتاليز هيتفاعل مع ال + +587 +00:46:16,760 --> 00:46:19,720 +hydrogen peroxide و يحوله بالمعادلة اللي بالدم و + +588 +00:46:19,720 --> 00:46:24,640 +في الوسط هنا إلى ماء زاد مين؟ أكسيجين، الأكسيجين + +589 +00:46:24,640 --> 00:46:28,440 +بيطلع من وين؟ بيطلع من مي جاعد اللي هو ال hydrogen + +590 +00:46:28,440 --> 00:46:34,640 +peroxide، فهيطلع بشكل مين؟ بشكل فقعةالفحص positive + +591 +00:46:34,640 --> 00:46:37,480 +إذا كان تكوّن في قاعك، الفحص negative إذا لم + +592 +00:46:37,480 --> 00:46:41,380 +يتكوّن في قاعك، تفهمين إيه السهل الفحص؟ one minute + +593 +00:46:41,380 --> 00:46:47,820 +test لكن .. لكن وظائفه وأهميته كبيرة جدا، ميزة بين + +594 +00:46:47,820 --> 00:46:51,180 +أستاذ فليك وكاي وبين أستاذ فليك وكاي، وطبعا + +595 +00:46:51,180 --> 00:46:55,540 +التفاصيل الأخرى هتعلموها بن وعمل إن شاء الله طيب + +596 +00:46:55,540 --> 00:47:00,260 +هنا يعني ..بالنقطة الأخيرة غطينا العوامل اللى + +597 +00:47:00,260 --> 00:47:06,340 +بتأثر على نمو البكتيريا البكتيريا تنقسم أو تتكاثر + +598 +00:47:06,340 --> 00:47:09,760 +بال simple binary region شوفوا الشكل اللى قدامكوا + +599 +00:47:09,760 --> 00:47:14,700 +هذاالمادة الوراثية في البكتيريا اللي هو الكروموزوم + +600 +00:47:14,700 --> 00:47:20,640 +صح انقسمت في البداية وكل واحد أخد طرف كل كروموزوم + +601 +00:47:20,640 --> 00:47:26,280 +أخد طرف وبدأ يتكون سبتا سبتا حاجز أو septum تمام و + +602 +00:47:26,280 --> 00:47:32,080 +بدأ يتكون حاجز الحاجز اجتمل و بعدين صار في انقسام + +603 +00:47:32,080 --> 00:47:39,260 +خلوي تعمل مين الأم اللي ولدت؟ اي واحدة منهم؟ هاد + +604 +00:47:39,260 --> 00:47:40,280 +الأم ولا هاد الأم؟ + +605 +00:47:45,440 --> 00:47:51,080 +two daughter cells يعني كل واحدة منهم ماخدة من + +606 +00:47:51,080 --> 00:47:55,040 +القديم و ماخدة منها من الجديد two daughter cells + +607 +00:47:55,040 --> 00:48:00,080 +هذا انقسام هذه الصورة بتوضح لنا يعني بطريقة رائعة + +608 +00:48:00,080 --> 00:48:05,500 +جدا ان كيف ال chromosome بدأ يتجري إلى طرف من + +609 +00:48:05,500 --> 00:48:09,840 +طرفين الخلية و هذه المنطقة إذا شفتوا هذه ال cross + +610 +00:48:09,840 --> 00:48:13,420 +wall او ال septum بدأت تظهر في المنطقة هذه في + +611 +00:48:13,420 --> 00:48:14,380 +المنطقة هذه + +612 +00:48:17,080 --> 00:48:20,960 +إذا ما هي وسيلة الانقسام هي ال simple binary بجنس + +613 +00:48:20,960 --> 00:48:24,140 +وقلنا ببساطة إن ال chromosome بينقسم ويروح على + +614 +00:48:24,140 --> 00:48:26,800 +الجهة وعلى الجهة وبعدين يتكون حاجز طبعا بعض + +615 +00:48:26,800 --> 00:48:29,940 +البكتيريا بيبقوا متسقة ب بعضها okay بتنقسم لكن + +616 +00:48:29,940 --> 00:48:34,840 +بيبقوا متسقة والانقسام أو عدم الانتصاق اللي بيحدده + +617 +00:48:34,840 --> 00:48:38,920 +ال genetic material اللي موجودة في الخلية شوفتم + +618 +00:48:38,920 --> 00:48:39,700 +مرة دبلو كوكاي + +619 +00:48:49,520 --> 00:48:55,340 +الزمن اللازم للانقسام أو للتضاعف يسمى generation + +620 +00:48:55,340 --> 00:48:58,340 +time أو doubling time + +621 +00:49:07,960 --> 00:49:11,120 +ماني كومون بكتيريا ال generation time تبقى من + +622 +00:49:11,120 --> 00:49:17,900 +عشرين إلى ستين دقيقة تخيلوا بنات في الساعة تنقسم + +623 +00:49:17,900 --> 00:49:24,080 +البكتيريا تلت مرة طيب بدى ابيع سيارتي انا اليوم + +624 +00:49:24,080 --> 00:49:29,480 +مين اشتريه اعطيكوا + +625 +00:49:29,480 --> 00:49:35,020 +عرض تبع السيارةبدي أخد في اليوم الأول منكوا شاكل + +626 +00:49:35,020 --> 00:49:38,140 +اللي بتشتريه هبدأ أعطيني شاكل و اليوم التاني بس + +627 +00:49:38,140 --> 00:49:44,440 +يضعف النبلة لإتنين شاكل و تلاتين يوم تلاتين يوم و + +628 +00:49:44,440 --> 00:49:47,340 +تصوّع أنه في اليوم الأول بدي أخد شاكل و التاني يوم + +629 +00:49:47,340 --> 00:49:51,560 +بدي أخد كمان اتنين تلت يوم أربعة لتلاتين يوم مين + +630 +00:49:51,560 --> 00:49:57,000 +يشتري اتفقنا بدأت اشتري .. خلاص اتفقنا على المبلة + +631 +00:49:58,230 --> 00:50:02,370 +راتبك من هنا لخمسين سنة ان شاء الله بت .. بت .. + +632 +00:50:02,370 --> 00:50:07,310 +لإنه احسبوا الرقم هتلاقوه رقم مهول جدا بقى اتنين + +633 +00:50:07,310 --> 00:50:10,390 +اص تلاتين اتنين اص تلاتين اللي مع أقل حسبة ممكن + +634 +00:50:10,390 --> 00:50:13,850 +تتطلع عليه و تشوف اتنين اص تلاتين كدهش بيطلع الرقم + +635 +00:50:13,850 --> 00:50:19,230 +البكتيريا بتتضاع في الساعة الواحدة بعض نوع من + +636 +00:50:19,230 --> 00:50:24,190 +البكتيريا تلت مرات فخلال اربع وعشرين ساعة اربع + +637 +00:50:24,190 --> 00:50:29,420 +وعشرين في تلاتة اتنين وسبعين72 مرة حطوا 2 قص 72 + +638 +00:50:29,420 --> 00:50:33,080 +شوفوا الرقم اللي بيطلع مليون .. بلايين من ال micro + +639 +00:50:33,080 --> 00:50:35,860 +organism وبالتالي احنا لما نزرع البكتيريا على + +640 +00:50:35,860 --> 00:50:40,220 +الطبق نزرع خلية واحدة غريبة نزرع خلية واحدة بعد 24 + +641 +00:50:40,220 --> 00:50:44,420 +ساعة بنصير نشوف المستعمرات طب البكتيريا حجمها واحد + +642 +00:50:44,420 --> 00:50:47,720 +مايكرونعشان انا اشوف العادات بتكون بالملي مترات + +643 +00:50:47,720 --> 00:50:50,780 +تمام؟ فعشان اساسي الملي مترات لازم تكون العادات + +644 +00:50:50,780 --> 00:50:56,520 +بالبلايين بالبلايين تمام؟ فواضح انه زمن الانقسام + +645 +00:50:56,520 --> 00:51:00,400 +في البكتيريا طبعا بتأثر بعوامل كثيرة فاكرين الظروف + +646 +00:51:00,400 --> 00:51:03,180 +اللي حكينا؟ لو ان ال temperature عشرة او ال + +647 +00:51:03,180 --> 00:51:05,740 +temperature خمستاشر اعتقد ال generation time + +648 +00:51:05,740 --> 00:51:10,840 +هيختلفلأنه بيأثر على مين؟ على النمو مش معقول أقول + +649 +00:51:10,840 --> 00:51:13,280 +generation time ل minimum و generation time ل + +650 +00:51:13,280 --> 00:51:17,380 +maximum ال generation time غالبا بنقول ل مين؟ لل + +651 +00:51:17,380 --> 00:51:22,340 +optimum يعني أفضل الظروف بنوفرها للبكتيريا و بنحسب + +652 +00:51:22,340 --> 00:51:26,400 +ال generation time و أقول لكم أنه في بعض أنواع من + +653 +00:51:26,400 --> 00:51:30,140 +البكتيريا بنختمها للمعلومة ال generation time قد + +654 +00:51:30,140 --> 00:51:34,120 +يصل إلى فقط عشر دقائق و نكمل المرة القادمة + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4.srt new file mode 100644 index 0000000000000000000000000000000000000000..3137b1a9e15816440700b4ef5378207690aaafb4 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4.srt @@ -0,0 +1,2398 @@ +1 +00:00:20,650 --> 00:00:24,270 +بسم الله الرحمن الرحيم نكمل موضوع الpathogenesis + +2 +00:00:24,270 --> 00:00:29,570 +للبكتيريا واليوم هتكلم عن جزء مهم جدا المتعلق بال + +3 +00:00:29,570 --> 00:00:33,550 +invasion هذا الجزء يأتي بعد الvirulence factors + +4 +00:00:33,550 --> 00:00:36,010 +الأساسي اللي درسناه اللي هو الattachment أو ال + +5 +00:00:36,010 --> 00:00:41,070 +adherence ودرسنا مجموعة من العوامل تحت هذا ال + +6 +00:00:41,070 --> 00:00:43,470 +factor واليوم هتكلم إن شاء الله عن مجموعة من ال + +7 +00:00:43,470 --> 00:00:46,890 +factors اللي بتساهم في الinvasion للبكتيريا + +8 +00:00:48,480 --> 00:00:52,320 +أنا ذكرت مثالين الماضية في نهاية المحاضرة مثال + +9 +00:00:52,320 --> 00:00:57,620 +السلمونيلة اللي بتاعتي فيها وكنا بتدخل عبر الأمعاء عبر + +10 +00:00:57,620 --> 00:01:01,660 +الفم إلى الأمعاء وبتخترق جدار الأمعاء عبر ال + +11 +00:01:01,660 --> 00:01:06,560 +lymph nodes وبتروح على الدم وبتنتشر من الدم إلى + +12 +00:01:06,560 --> 00:01:10,680 +organs متنوعة ومختلفة وسميناها invasive + +13 +00:01:10,680 --> 00:01:15,300 +microorganism بينما تحدثنا بالمثال المقابل التيتانوس + +14 +00:01:15,300 --> 00:01:19,850 +وكنا بتبقى في نفس المكان اللي .. اللي .. اللي عملت + +15 +00:01:19,850 --> 00:01:22,770 +فيه infection بينما الtoxin تبعها هو اللي بينتقل + +16 +00:01:22,770 --> 00:01:29,190 +إلى أماكن متنوعة فالآن احنا هنتطرق لبعض العوامل و + +17 +00:01:29,190 --> 00:01:33,470 +أعتقد أنه طرقنا إليها بمرور سريع نحاول المرة مرة + +18 +00:01:33,470 --> 00:01:37,850 +أخرى قبل ما نبدأ في محاضرتنا إن شاء الله لجينا إن + +19 +00:01:37,850 --> 00:01:42,670 +في مجموعة كبيرة من الinvasive factors هم عبارة عن + +20 +00:01:42,670 --> 00:01:48,870 +إنزيم زي الhyaluronidase زي الcollagenase زي ال + +21 +00:01:48,870 --> 00:01:53,410 +coagulase زي اللي هو الـ .. الـ .. الkinases والـ + +22 +00:01:53,410 --> 00:01:58,160 +neuraminidases والphospholipases والlecithinases + +23 +00:01:58,160 --> 00:02:03,120 +مظبوط إن هما ليسوا عبارة عن إنزيم وهذه الإنزيمات + +24 +00:02:03,120 --> 00:02:07,340 +لها خطوة محددة أو specific step تمثلًا ال + +25 +00:02:07,340 --> 00:02:09,740 +hyaluronidase بيفك الhyaluronic acid والـ + +26 +00:02:09,740 --> 00:02:12,560 +hyaluronic acid هو اللي مسؤول عن ربط الconnective + +27 +00:02:12,560 --> 00:02:17,740 +tissue البعض البعض الcollagenases اللي برضه + +28 +00:02:17,740 --> 00:02:21,160 +بتفكك الكولاجين اللي برضه بربط الmuscles ببعضهم + +29 +00:02:21,160 --> 00:02:25,150 +ببعض لما نفكر في خلية عن بعضها البعض، إيه اللي + +30 +00:02:25,150 --> 00:02:30,590 +بيصير؟ مثلًا عندي فقدان .. مثلًا عندي فقدان للارتباط + +31 +00:02:30,590 --> 00:02:33,390 +بين خلية وخلية وبالتالي بيصير في عندي الblock + +32 +00:02:33,390 --> 00:02:38,490 +supply لهذه الخلية بينقطع وبالتالي في عندي death + +33 +00:02:38,490 --> 00:02:43,140 +لهذه الخلية الكواجيليز ليه أصفنا إنه بيعمل ال + +34 +00:02:43,140 --> 00:02:47,660 +fibrinogen إلى الfibrin واللي هي الclot وهالـclot + +35 +00:02:47,660 --> 00:02:51,500 +ممكن تتجمع في أماكن مختلفة في الجسم ويتم ال + +36 +00:02:51,500 --> 00:02:55,200 +plugging إغلاق لبعض الcapillaries أو الveins حتى + +37 +00:02:55,200 --> 00:02:59,820 +وبالتالي تقل blood supply على areas على مناطق + +38 +00:02:59,820 --> 00:03:06,380 +كاملة Loss of blood supply الليوكوسايدين + +39 +00:03:06,380 --> 00:03:10,460 +والهيموليسين متخصصات في قتل نوع معين من الخلايا + +40 +00:03:10,460 --> 00:03:14,200 +النوع الأول اللي هو الليوكوسايدين وليوكوسايدز + +41 +00:03:14,200 --> 00:03:17,140 +والثانية اللي هي الإريثروسايدز كلمة هيموليسين تما + +42 +00:03:17,140 --> 00:03:23,220 +الهيموليسس للred blood cells وآخر شيء هدفنا عن نوعين + +43 +00:03:23,220 --> 00:03:28,600 +من الtoxins أحد اسمها anthrax edema factor أفن + +44 +00:03:28,600 --> 00:03:32,960 +edema factor والثاني pertussis adenylate cyclase + +45 +00:03:32,960 --> 00:03:36,320 +وكانوا الاثنين بيشتغلوا بنفس القدرة الاثنين + +46 +00:03:36,320 --> 00:03:40,540 +بيعملوا كتاليز يا بنات بيعملوا activation لأنزيم + +47 +00:03:40,540 --> 00:03:46,060 +اسمه adenylate cyclase enzyme هذا الإنزيم بيحول ال + +48 +00:03:46,060 --> 00:03:52,370 +ATP إلى cyclic AMP الـCyclic AMP بيعمل عملية + +49 +00:03:52,370 --> 00:03:56,130 +increase للproduction of electrolytes .. مهم + +50 +00:03:56,130 --> 00:04:00,530 +بيعمل الproduction هذا extracellularly .. إيش + +51 +00:04:00,530 --> 00:04:04,030 +يعني extracellularly؟ يعني خارج الخلية .. لأن لما + +52 +00:04:04,030 --> 00:04:08,210 +يصير فيه تركيز عالية من الelectrolytes خارج + +53 +00:04:08,210 --> 00:04:13,790 +الخلية، الجسم يميل إلى تخفيف .. إلى تخفيف هذه ال + +54 +00:04:13,790 --> 00:04:16,790 +electrolytes عبر إنتاج كمية كبيرة من مين؟ من + +55 +00:04:16,790 --> 00:04:22,360 +السائل إذا كان الelectrolyte موجودة في تجويف ال + +56 +00:04:22,360 --> 00:04:26,760 +human زي مثلًا الintestine، بينتج عندي داء الإسهال لأنه + +57 +00:04:26,760 --> 00:04:30,980 +سائل كمية كبيرة، إذا كان ما لهوش تجويف، بيصير عندي + +58 +00:04:30,980 --> 00:04:34,140 +وذمة أو الاستسقاء، اللي هي الانتفاخ الناتجة عن + +59 +00:04:34,140 --> 00:04:40,420 +وجود كمية كبيرة من مين؟ من الانتفاخ الثالث + +60 +00:04:40,420 --> 00:04:44,240 +من الvirulence factors، اللي هو من التوكسين احنا + +61 +00:04:44,240 --> 00:04:48,740 +قررنا نعامل صنفين based on the mechanism of + +62 +00:04:48,740 --> 00:04:52,260 +production endotoxins are integral part of the + +63 +00:04:52,260 --> 00:04:56,700 +cell component يعني الجزء الأساسي ولا تتحلل ولا + +64 +00:04:56,700 --> 00:05:01,300 +تطلق إلا بعد موت الخلية بينما الexotoxins are + +65 +00:05:01,300 --> 00:05:04,900 +produced while the cells are alive عندما تكون + +66 +00:05:04,900 --> 00:05:12,380 +الخلية حية فحية وبتنتج طيب تخيلوا الآن أنها أنتجت + +67 +00:05:12,380 --> 00:05:18,280 +التوكسين تبعها في غذائنا غير علمًا تنتجه داخل + +68 +00:05:18,280 --> 00:05:23,420 +أجسامنا وبعض الtoxins قد تنتج في داخل أجسامنا لكن + +69 +00:05:23,420 --> 00:05:27,240 +اتضال التأثير تبعها محصور في مكان محدد طبعًا + +70 +00:05:27,240 --> 00:05:31,640 +المصطلحات اللي على السطر produced in vitro وin + +71 +00:05:31,640 --> 00:05:33,820 +vivo بعرف المصطلحات هذه كم ولا إنكم ولا لأ + +72 +00:05:33,820 --> 00:05:41,970 +نترجمهم in vivo يعني إيش يعني؟ in test tube يعني + +73 +00:05:41,970 --> 00:05:49,190 +ليست في كائن حي معمليًا in vivo داخل الكائن الحي + +74 +00:05:49,190 --> 00:05:52,750 +فلما بنعمل experiment احنا بنسميها experiment إما + +75 +00:05:52,750 --> 00:05:55,970 +بنقول in vivo أو in vitro حسب وين إحنا أجرينا ال + +76 +00:05:55,970 --> 00:05:59,310 +experiment إذا عملناها في الtest tube عملناها في + +77 +00:05:59,310 --> 00:06:03,490 +الpetri dish دماغ بنقول عنها in vitro إذا عملناها + +78 +00:06:03,490 --> 00:06:09,010 +في الإنسان أو الحيوان بنسميها in vivo فإذا الـ + +79 +00:06:09,010 --> 00:06:12,730 +toxin produced in vivo ممكن يسبب food poisoning و + +80 +00:06:12,730 --> 00:06:15,230 +من أشهر الأمثلة الbotulinum toxin أو الbotulism + +81 +00:06:15,230 --> 00:06:19,690 +toxin والاستافيلوكوكال enterotoxin كلمة enterotoxin + +82 +00:06:19,690 --> 00:06:24,830 +الآن صرنا مصنفين مصطلح جديد كنا مصنفين exo وendo والآن + +83 +00:06:24,830 --> 00:06:28,210 +بنتكلم عن entero والentero عبارة عن تصنيف لل + +84 +00:06:28,210 --> 00:06:32,530 +location where the toxin acts when it is some + +85 +00:06:35,740 --> 00:06:40,280 +بيؤثر على الcentral nervous system بنسميه neurotoxin، + +86 +00:06:40,280 --> 00:06:46,400 +حسب مكان العمل، حسب مكان العمل، طب إذا بقتل كل + +87 +00:06:46,400 --> 00:06:50,180 +الخلايا، بنسميه cytotoxin + +88 +00:06:53,540 --> 00:06:58,360 +أو مصطلحات nephrotoxic يعني سام لمن؟ لخلايا الكلى + +89 +00:06:58,360 --> 00:07:05,000 +ototoxic سام للأذن يعني للعصب السمعي مثلًا في + +90 +00:07:05,000 --> 00:07:09,920 +الأذن بيكون متخصص لكن في النهاية هو تصنيف بحسب ال + +91 +00:07:09,920 --> 00:07:14,140 +site affected فاتفقنا + +92 +00:07:14,140 --> 00:07:16,780 +على الenterotoxin والbotulism وأنا بقول لكم ال + +93 +00:07:16,780 --> 00:07:21,980 +botulism هو أقوى سم معروف للإنسان ينتج من قبل + +94 +00:07:21,980 --> 00:07:28,480 +كائن حي طبعًا بتسمم الناس عبر تناول معلبات + +95 +00:07:28,480 --> 00:07:35,980 +المعلبات اللي بتكون مصنعة ولم نصل فاكرين لما + +96 +00:07:35,980 --> 00:07:39,780 +اتكلمت عن الsterilization وقلنالكوا إن في مصطلح + +97 +00:07:39,780 --> 00:07:43,920 +اسمه commercial sterilization تعقيم تجاري لأن + +98 +00:07:43,920 --> 00:07:48,220 +التعقيم العادي ممكن يقتل لكن ما يقتلش .. بظبط + +99 +00:07:48,220 --> 00:07:50,060 +فبالتالي + +100 +00:07:51,370 --> 00:07:57,950 +بصراحة تعقيم تجاري بيقتل خلو الطعام من الspores + +101 +00:07:57,950 --> 00:08:02,370 +الممرضة اللي من ضمنها مين؟ البوتلوني طيب produced + +102 +00:08:02,370 --> 00:08:07,130 +in vivo يعني داخل جسمنا احنا تناولنا البكتيريا و + +103 +00:08:07,130 --> 00:08:10,010 +البكتيريا نمت جوا وبدأت تنتج الtoxin تبعها + +104 +00:08:10,010 --> 00:08:15,310 +داخلنا زي مين؟ زي الدفتيريا من أحد أشهر وأخطر + +105 +00:08:15,310 --> 00:08:21,220 +الأمراض قبل 30 سنة وانزلوا كان الدفتيريا واحد من + +106 +00:08:21,220 --> 00:08:25,340 +الأمراض الخطيرة جدا أو أربعين سنة حتى اللي هو + +107 +00:08:25,340 --> 00:08:27,820 +الخناق بيسموه باللغة العربية تمام؟ أو العرب + +108 +00:08:27,820 --> 00:08:30,100 +بيسموها دفتيريا اللي هو الدفتيريا + +109 +00:08:33,130 --> 00:08:36,090 +اللي قلت لكم من كتر ما الـ .. الـ .. الـ .. + +110 +00:08:36,090 --> 00:08:40,970 +الانقباضات بتكون عنيفة قد ينكسر العمود الفقري + +111 +00:08:40,970 --> 00:08:44,710 +للمريض، تخيل العمود الفقري من .. عشان نكسره بدنا + +112 +00:08:44,710 --> 00:08:50,250 +قوة هائلة، لكن من شدة انقباض العضلات في المريض + +113 +00:08:50,250 --> 00:08:54,890 +اللي عندنا نفسه قد ينكسر العمود الفقري كانها الـ + +114 +00:08:54,890 --> 00:08:58,690 +streptococcal erythrogenic toxin، كلها بالثلاث + +115 +00:08:58,690 --> 00:09:01,730 +أنواع من الtoxins اللي ذكرناها دول الأمثلة، تنتج + +116 +00:09:01,730 --> 00:09:05,470 +in vitro، أفضل in vivo، والتأثير بتاعها بيكون + +117 +00:09:05,470 --> 00:09:09,290 +systemic، إيه يعني systemic؟ يعني بكل الجسم، بكل + +118 +00:09:09,290 --> 00:09:14,010 +الجسم، بيبقى هناك بعض الميكروبات والtoxins بيكون + +119 +00:09:14,010 --> 00:09:20,980 +لها local toxic effect، لأ الtoxins بنا systemic، + +120 +00:09:20,980 --> 00:09:23,880 +البكتيريا نفسها local، البكتيريا نفسها localized، بس + +121 +00:09:23,880 --> 00:09:28,560 +في الlocalized الـ .. البكتيريا والtoxins زي + +122 +00:09:28,560 --> 00:09:31,880 +الكوليرا، الكوليرا في الأمعاء مابتعزلهاش من الدم + +123 +00:09:31,880 --> 00:09:34,380 +ولا بتعزلها من أي مكان تاني، فقط من وين؟ من + +124 +00:09:34,380 --> 00:09:37,400 +الأمعاء، فالتوكسين تبعها أيضًا بيكون تأثيره على + +125 +00:09:37,400 --> 00:09:42,390 +الأمعاء بنعيش الإشيريشيا كولاي هي non pathogenic في ال + +126 +00:09:42,390 --> 00:09:44,830 +intestinal tract لكن في منها exceptions أربع + +127 +00:09:44,830 --> 00:09:47,110 +أصناف، هم كلها لاحقا، هم كلها بعد medical + +128 +00:09:47,110 --> 00:09:51,590 +microbiology واحدة منهم اسمها Enterotoxigenic + +129 +00:09:51,590 --> 00:09:56,110 +Escherichia Coli، إذا الآن قدامنا على اللوحة تلت + +130 +00:09:56,110 --> 00:10:00,370 +نماذج، يا ريت نطلع عليهم كويس إذا ال toxin was + +131 +00:10:00,370 --> 00:10:04,070 +produced in vitro، إذا ال toxin was produced in + +132 +00:10:04,070 --> 00:10:09,910 +vivo، في مثلين، systemic و في أنها local الآن أنا + +133 +00:10:09,910 --> 00:10:14,530 +كنت في تصنيف ال toxins حد بيسموها A B Model ال A B + +134 +00:10:14,530 --> 00:10:18,690 +Model عبارة عن نموذج لتوكسين أو مجموعة توكسينات + +135 +00:10:18,690 --> 00:10:23,530 +خاصة، مش خاصة هي ال Exotoxins مكوّنة من شقين، بدا + +136 +00:10:23,530 --> 00:10:28,570 +الشق الأول بنسميه A واحفظه A Active والجزء الثاني + +137 +00:10:28,570 --> 00:10:31,290 +B اللي هو زي ما موجود على السبورة أيضا + +138 +00:10:34,430 --> 00:10:37,650 +واطلع على ال animation اللي موجودة على يمين الشاشة + +139 +00:10:37,650 --> 00:10:42,250 +هلاقي إنه في خلية فيها receptor لمين؟ ال receptor ل + +140 +00:10:42,250 --> 00:10:48,170 +B لكن عمل internalization لمن؟ ل A و B هتكلم عنها + +141 +00:10:48,170 --> 00:10:54,670 +الآن هي B وهي A وهي ال receptor الارتباط لمن هيكون + +142 +00:10:54,670 --> 00:11:00,730 +الآن ل B لكن ال internalization بتتم لكل التوكسين + +143 +00:11:00,730 --> 00:11:05,330 +لكلهما بيصير release مين الآن اللي بيشتغل جوا؟ B + +144 +00:11:05,330 --> 00:11:10,330 +ولا A؟ B has no independent action ما لهوش أي عمل + +145 +00:11:10,330 --> 00:11:14,830 +مستقل، فقط عملية إيصال التوكسين إلى المكان الموجود + +146 +00:11:14,830 --> 00:11:18,890 +طبعا العملية برضه كمان مهمة، يعني لو شلنا ال B من ال + +147 +00:11:18,890 --> 00:11:20,390 +A مش هيشتغل + +148 +00:11:23,360 --> 00:11:27,640 +موضحة النقطة دي إنه في عندي في ال toxin A B Model + +149 +00:11:27,640 --> 00:11:33,540 +A هو ال active و ال B بس بدنا نفهمش هنا ال + +150 +00:11:33,540 --> 00:11:40,540 +A نفترض إنها في المنتصف و ال B ممكن تكون فجأة + +151 +00:11:40,540 --> 00:11:43,540 +تحتها، على يمينها، على يسارها، ما احنا حسب ما ننظر إليها + +152 +00:11:43,540 --> 00:11:48,960 +طيب لو كانت ال A في المنتصف وفي عندي B و B و B و + +153 +00:11:48,960 --> 00:11:50,460 +B يعني أكتر من B + +154 +00:11:56,020 --> 00:12:01,260 +النسخ بيصير فرصة .. فرصة أن ال toxin يرتبط أسرع و + +155 +00:12:01,260 --> 00:12:05,120 +أسهل، تمام؟ بالضبط .. بالضبط زي لو احنا بنجيب كورة + +156 +00:12:05,120 --> 00:12:09,380 +و ن .. و ن .. و نلحم فيها مسامير ونرميها على أي + +157 +00:12:09,380 --> 00:12:13,160 +جهة، رميناها هلاقي مسامير وسط الأرض و مسامير + +158 +00:12:13,160 --> 00:12:16,600 +أطرافها في .. في اتجاهات متنوعة ومختلفة، وبالتالي + +159 +00:12:16,600 --> 00:12:21,510 +فرصتها في الارتباط بتكون إيه؟ أعلى، طيب برضه نسميه a + +160 +00:12:21,510 --> 00:12:25,890 +b model صحيح، a one و five b's تمام، لكن في الآخر هو + +161 +00:12:25,890 --> 00:12:31,030 +a و b، طب لو كان a اتنين وخمسة b بيمشي الحال، بيمشي + +162 +00:12:31,030 --> 00:12:35,810 +الحال، بيبقى نفس المفهوم، two a's تمام، ويمكن a one + +163 +00:12:35,810 --> 00:12:39,770 +و a two، خدوا بالكم a one و a two إيش معنى إن a + +164 +00:12:39,770 --> 00:12:46,420 +بيختلف عن a one، بيختلف عن a two A1, A2, 5B's، الـ + +165 +00:12:46,420 --> 00:12:50,240 +5B's هم هما وممكن زي ما حضرتك اتوقعتي إنه نلاقي A + +166 +00:12:50,240 --> 00:12:54,960 +واحدة و 5B's مختلفين وهذا بيصير .. إيش بيعمل هذا؟ + +167 +00:12:54,960 --> 00:13:00,560 +بيزيد من ال specificity بتاعت للأنشجة المختلفة، بيصير + +168 +00:13:00,560 --> 00:13:04,500 +قدرة التوكسينة على الدخول إلى أنسجة متنوعة أعلى + +169 +00:13:04,500 --> 00:13:07,620 +لما يكون في عندها اتنين في ال B أو في ال binding + +170 +00:13:07,620 --> 00:13:13,920 +site من النماذج المشهورة جدا في علم ال microbiology + +171 +00:13:13,920 --> 00:13:17,140 +على ال mechanism of action ل A-B model اللي هو + +172 +00:13:17,140 --> 00:13:22,060 +Diphtheria toxin وطبعا في شبيه له اسمه Pseudomonas + +173 +00:13:22,060 --> 00:13:26,420 +aeruginosa toxin A أو X-toxin A كيف بيشتغلوا + +174 +00:13:26,420 --> 00:13:30,160 +دولها؟ تقريبا بيشتغلوا بنفس الطريقة بس خدنا ناخد + +175 +00:13:30,160 --> 00:13:33,720 +ال Diphtheria كنموذج والثاني بنقول مثله بالضبط + +176 +00:13:33,720 --> 00:13:40,240 +تعرف ال protein synthesis؟ ال protein synthesis الـ + +177 +00:13:40,240 --> 00:13:43,400 +protein synthesis بيتلخص بإنه في عندي large + +178 +00:13:43,400 --> 00:13:47,720 +subunit و small subunit و messenger mRNA وبيصير + +179 +00:13:47,720 --> 00:13:52,500 +ارتباط بين الثلاث components دول وبيبدأ ال .. ال + +180 +00:13:52,500 --> 00:13:55,700 +.. ال aminoacyl transfer يجي ينزل على ال ribosome + +181 +00:13:55,700 --> 00:13:58,640 +ويفرّغ حمولته من ال amino acid وال amino acid + +182 +00:13:58,640 --> 00:14:03,180 +يرتبط، ينتقل لـ aminoacyl و هكذا ويصير في إطالة + +183 +00:14:03,180 --> 00:14:09,380 +للسلسلة كلمة elongation factor بتعني عامل يساهم في + +184 +00:14:09,380 --> 00:14:15,980 +إطالة ال polypeptide اللي هو ال EF وهذا العامل من + +185 +00:14:15,980 --> 00:14:19,740 +ضرورات ال protein synthesis من ضرورات ال protein + +186 +00:14:19,740 --> 00:14:26,080 +synthesis في عملية اللي هو ال translation طيب + +187 +00:14:26,080 --> 00:14:29,240 +تخيلوا معي الوضع كان ثاني، ال Diphtheria toxin + +188 +00:14:29,240 --> 00:14:34,880 +بتروح ترتبط بهذا ال factor وبتضيف إليه ADP-ribose + +189 +00:14:34,880 --> 00:14:40,480 +وعلى اللي بنسميها ADP-ribosylation ADP اللي هو + +190 +00:14:40,480 --> 00:14:49,840 +adenosine diphosphate الآن ضفنا ADP-ribose لمين؟ + +191 +00:14:49,840 --> 00:14:55,640 +لل elongation factor 2 اللي كان active لما ضفنا له + +192 +00:14:55,640 --> 00:15:00,120 +بطل active لما بطل active يبقى الآن بطل يكون في + +193 +00:15:00,120 --> 00:15:03,910 +دورة تصنيع لبروتين طيب بالنسبة لي احنا بنتكلم + +194 +00:15:03,910 --> 00:15:07,610 +دايما هو باستمرار، خلية بدون protein synthesis is a + +195 +00:15:07,610 --> 00:15:12,210 +doomed dead يعني محكوم عليها بالموت، لماذا محكوم + +196 +00:15:12,210 --> 00:15:15,910 +عليها بالموت؟ كل ال enzymes اللي بتشتغل في الخلية + +197 +00:15:15,910 --> 00:15:19,990 +هي proteins وبالتالي بدون تصنيع enzymes أو other + +198 +00:15:19,990 --> 00:15:24,770 +components الخلية بالموت، وبالتالي أي toxin + +199 +00:15:27,350 --> 00:15:33,450 +بيواجه تصنيع ال protein irreversibly بشكل غير قابل + +200 +00:15:33,450 --> 00:15:37,530 +للعكس، غير قابل للعكس، غالبا .. غالبا بيؤدي لموت + +201 +00:15:37,530 --> 00:15:38,710 +اللي هو إيه؟ الخلية + +202 +00:15:58,120 --> 00:16:00,920 +طب طبطبطبطبطبطبطبطبطبطبطبطبطبطبطبطبطبطبط + +203 +00:16:15,080 --> 00:16:19,360 +بتتّصل بال adenylate cyclase enzyme، اتصالها بال + +204 +00:16:19,360 --> 00:16:22,000 +adenylate cyclase enzyme مش بيعمل، بيعمله + +205 +00:16:22,000 --> 00:16:26,360 +activation لها، عكس الأولانية اللي كانت بتعمل له + +206 +00:16:26,360 --> 00:16:30,400 +inactivation، إذا بالاتصال، خدوا بالكم، إن التوكسين + +207 +00:16:30,400 --> 00:16:32,780 +في المركب يا بيعمل له activation يا بيعمل له + +208 +00:16:32,780 --> 00:16:37,120 +inactivation، فهذا عمل له activation، لما عمل له + +209 +00:16:37,120 --> 00:16:42,480 +activation، راح حول ال ATP ل cyclic AMP وال cycle + +210 +00:16:42,480 --> 00:16:46,980 +kmb انشرحت المرة الماضية، قلنا بيزيد من اللي هيطلع + +211 +00:16:46,980 --> 00:16:52,080 +على السبورة، على اللوحة أيضا، إن هنا كمية الإنتاج + +212 +00:16:52,080 --> 00:16:56,480 +لل Sodium ول chloride ول potassium ول + +213 +00:16:56,480 --> 00:17:01,220 +carbonate زادت في ال human of the intestine فالجسم + +214 +00:17:01,220 --> 00:17:05,800 +مضطر ينزل معاه كمان كمية كبيرة من من الماء وبالتالي + +215 +00:17:05,800 --> 00:17:09,550 +إسهال واز produced إذا هذه الـ mechanism by which + +216 +00:17:09,550 --> 00:17:16,030 +ال Vibrio cholera produces إسهال، اكيوميليشن .. نجيب + +217 +00:17:16,030 --> 00:17:18,550 +معكوس، نقول إسهال was produced، ليش فيه إسهال؟ + +218 +00:17:18,550 --> 00:17:21,550 +لأنه فيه ماء، ليش فيه ماء كبيرة، كمية كبيرة؟ لأنه + +219 +00:17:21,550 --> 00:17:24,810 +فيه electrolytes طب ليش ال electrolytes اتراكمت في + +220 +00:17:24,810 --> 00:17:29,130 +المكان هذا؟ لأنه فيه كمية Cyclic AMP أنتجت بشكل + +221 +00:17:29,130 --> 00:17:32,730 +كبير، لماذا؟ لأنه فيه cell activation لليليت + +222 +00:17:32,730 --> 00:17:35,530 +ال cyclase enzyme، ميه اللي عمل activation؟ اللي هو + +223 +00:17:35,530 --> 00:17:41,150 +ال Cholera toxin وهناك Escherichia coli بتنتج + +224 +00:17:41,150 --> 00:17:45,250 +توكسين اسمه Cholera-like toxin اللي هي البكتيريا + +225 +00:17:45,250 --> 00:17:49,090 +اللي اسمها Enterotoxigenic Escherichia coli + +226 +00:17:49,090 --> 00:17:54,090 +Enterotoxigenic Escherichia coli وبين قوسين حطوا + +227 +00:17:54,090 --> 00:17:57,730 +كلمة labile ما أعرفش مولود على اللوحة كلمة labile + +228 +00:17:57,730 --> 00:18:04,970 +شايفينها؟ E. coli labile toxin كلمة labile يعني قابل + +229 +00:18:04,970 --> 00:18:10,230 +للتدمير .. قابل للتدمير، لما عزلوا ال E.coli toxins + +230 +00:18:10,230 --> 00:18:14,370 +لجوا منها نوعين، لجوا نوع stable التوكسين تبعها لما + +231 +00:18:14,370 --> 00:18:19,110 +نحطه بالحرارة ما يتأثرش، فسموه S أو stable toxin + +232 +00:18:19,110 --> 00:18:24,070 +والثاني بتأثر بالحرارة فسموه labile toxin، إذا ال + +233 +00:18:24,070 --> 00:18:25,930 +labile toxin هو اللي بنتكلم عنه + +234 +00:18:32,130 --> 00:18:36,110 +يعني هيمروا معها في المستقبل oxygen labile هداك + +235 +00:18:36,110 --> 00:18:39,610 +هنقوله عنها heat labile تتأثر بالحرارة أو تتأثر + +236 +00:18:39,610 --> 00:18:48,590 +بمين؟ بال oxygen طيب نجي لتوكسين تنتجه الشيجيلا أو + +237 +00:18:48,590 --> 00:18:50,950 +برضه نوع آخر من الشرشيلا كولا يسمونه + +238 +00:18:50,950 --> 00:18:56,270 +Enterohemorrhagic-Escherichia-Kola إذن نتكلم عن two + +239 +00:18:56,270 --> 00:19:03,990 +toxins بينتلكوا نفس ال mechanism of برضه في المحصلة + +240 +00:19:03,990 --> 00:19:08,190 +بيوقفوا ال protein synthesis هنا لكن بطريقة مختلفة + +241 +00:19:08,190 --> 00:19:11,990 +عن الدفتيريا، تتذكروا الدفتيريا اشتغلت على مين؟ على + +242 +00:19:11,990 --> 00:19:16,710 +elongation factor 2 هذه اشتغلت على مين؟ على إنها + +243 +00:19:16,710 --> 00:19:20,570 +عملت تكسير للريبوزوم RNA اللي في الريبوزوم + +244 +00:19:20,570 --> 00:19:22,990 +المهمين اللي بيصنع ال protein synthesis أساسا + +245 +00:19:22,990 --> 00:19:26,630 +الريبوزوم، إذا خربنا الريبوزوم وبقوا فينا ال + +246 +00:19:26,630 --> 00:19:29,690 +protein synthesis يبقى وجفنا وأصنع فردة ال + +247 +00:19:29,690 --> 00:19:37,430 +protein لكن بطريقة مختلفة عن اللي هو ال .. نعم، نجد + +248 +00:19:37,430 --> 00:19:42,190 +البوتولينيوم توكسين البوتولينيوم توكسين محتاج مننا + +249 +00:19:42,190 --> 00:19:46,600 +يمكن إن شاء الله نقدر نوريكم ال animation بنطلع على + +250 +00:19:46,600 --> 00:19:49,940 +ال animation اللي قدامنا هذه ونشوف ال X-Toxins + +251 +00:19:49,940 --> 00:19:53,700 +طبعا قلنا they are produced while the cells are + +252 +00:19:53,700 --> 00:19:58,180 +alive and their effect may be localized or may be + +253 +00:19:58,180 --> 00:20:01,780 +systemic على حسب اللي هو النوع التوكسين زي ال + +254 +00:20:01,780 --> 00:20:03,980 +Vibrio كده اللي قلنا localized بينما التتانوس كان + +255 +00:20:03,980 --> 00:20:09,580 +إيه؟ كان systemic تصنيف الtoxins قلنا ممكن يكون + +256 +00:20:09,580 --> 00:20:15,340 +حسب اللي هو الsite affected يعني مثلا لما قلنا انه + +257 +00:20:15,340 --> 00:20:21,060 +بصيب الnervous system سمنة neurotoxin إذا بصيب ال + +258 +00:20:21,060 --> 00:20:24,700 +intestine سمنة الintestine الintestine ال + +259 +00:20:24,700 --> 00:20:25,140 +intestine الintestine الintestine الintestine + +260 +00:20:25,140 --> 00:20:25,380 +intestine الintestine الintestine الintestine + +261 +00:20:25,380 --> 00:20:25,540 +الintestine الintestine الintestine ال + +262 +00:20:25,540 --> 00:20:26,000 +intestine الintestine الintestine الintestine + +263 +00:20:26,000 --> 00:20:26,980 +intestine الintestine الintestine الintestine + +264 +00:20:26,980 --> 00:20:27,020 +الintestine الintestine الintestine ال + +265 +00:20:27,020 --> 00:20:27,840 +intestine الintestine الintestine الintestine + +266 +00:20:27,840 --> 00:20:27,860 +intestine الintestine الintestine الintestine + +267 +00:20:27,860 --> 00:20:27,900 +intestine الintestine الintestine الintestine + +268 +00:20:27,900 --> 00:20:27,940 +intestine الintestine الintestine الintestine + +269 +00:20:27,940 --> 00:20:28,040 +الintestine الintestine الintestine ال + +270 +00:20:28,040 --> 00:20:28,320 +الintestine الintestine الintestine ال + +271 +00:20:28,320 --> 00:20:32,660 +الintestine الintestine الintestine ال + +272 +00:20:32,660 --> 00:20:36,340 +intestine ال + +273 +00:20:44,400 --> 00:20:47,540 +التصنيف الآن by structure and function هذا تصنيفه + +274 +00:20:47,540 --> 00:20:51,720 +كويس كثير احنا قلنا AB model صح الـ AB model إذا كان + +275 +00:20:51,720 --> 00:20:55,620 +تركيبه من قطعتين أساسيتين active و binding بيسمي + +276 +00:20:55,620 --> 00:20:59,280 +AB toxin وفي membrane disruption اللي هو بيأثر + +277 +00:20:59,280 --> 00:21:02,820 +على ال .. ال .. ال .. الmembrane خدنا ناخد الأول + +278 +00:21:02,820 --> 00:21:06,540 +حاجة اللي هو الـ AB toxin وطبعا الأخر اللي هو كان + +279 +00:21:06,540 --> 00:21:10,720 +super antigen هاي الـ A domain والجزء التاني هو الـ B + +280 +00:21:10,720 --> 00:21:13,260 +domain الـ B كان للbinding + +281 +00:21:16,520 --> 00:21:20,240 +احنا معناه نفهم الconcepts بشكل أساسي عشان نقدر + +282 +00:21:20,240 --> 00:21:24,080 +نفهم بعض النقاط المتعلقة على هذا الtoxin لاحقا + +283 +00:21:24,080 --> 00:21:27,860 +بالendosomes بعدين بيصير له release واللي هو الـ A + +284 +00:21:27,860 --> 00:21:29,940 +هو اللي بيقوم بالمهمة تبعه + +285 +00:21:37,250 --> 00:21:41,330 +طبعا الeffects مختلفة الeffects مختلفة بحسب + +286 +00:21:41,330 --> 00:21:44,530 +التوكسين لكن احنا بنوصل الفكرة ان الـ B هو اللي دخل + +287 +00:21:44,530 --> 00:21:48,190 +الـ A وبعدين صار له release الmembrane disruption + +288 +00:21:48,190 --> 00:21:51,910 +فكان الphospholipase والlycethinase وال + +289 +00:21:51,910 --> 00:21:55,210 +leukocydenes كل مدة بيشتغلوا على مين؟ على ال + +290 +00:21:55,210 --> 00:21:59,030 +membranes فلو بيعملوا complex معين بيفتح ال + +291 +00:21:59,030 --> 00:22:02,710 +membrane بيصير release لمين؟ للcomponent سبعة + +292 +00:22:02,710 --> 00:22:04,930 +الخلية هذا بيصير موت ليه؟ للخلية + +293 +00:22:08,300 --> 00:22:11,480 +النوع الثالث هو الsuper antigen وانا بدأ اشرح هو + +294 +00:22:11,480 --> 00:22:13,880 +يعني مش كتير واضح اللي .. اللي .. اللي موجود في ال + +295 +00:22:13,880 --> 00:22:18,120 +animation بس برضه كويس اننا نطلع عليه نجح فهنا + +296 +00:22:18,120 --> 00:22:24,640 +الآن لما أنا .. لو أنا في المحاضرة بدوزع حدث معين + +297 +00:22:24,640 --> 00:22:31,060 +على الطلاب فبقول لزميلاتكم انت عليكي واجب X وانت + +298 +00:22:31,060 --> 00:22:37,700 +واجب Y و Z و A و C بنصنف الموضوع لكن لو افترضنا .. + +299 +00:22:37,700 --> 00:22:42,480 +لو افترضنا ان الشخص اللي بوزع المهمة مش موجود و + +300 +00:22:42,480 --> 00:22:46,660 +اجا واحد انت عليك مهمة X وانت مهمة X وانت مهمة X + +301 +00:22:46,660 --> 00:22:50,740 +وانت مهمة X بيصير اللي بيعملوا نفس المهمة كثرة + +302 +00:22:50,740 --> 00:22:59,990 +فالمنتج تبع المهمة برضه كثرة احنا بنعرف .. بنعرف ان + +303 +00:22:59,990 --> 00:23:04,130 +ال .. ال .. الimmune system is orchestrated .. + +304 +00:23:04,130 --> 00:23:06,070 +orchestrated يعني منظم زي orchestra بتاخد + +305 +00:23:06,070 --> 00:23:08,710 +orchestra اللي بيبقى ماسك العصايا وي .. وينظم ال + +306 +00:23:08,710 --> 00:23:13,290 +.. ال .. اللي بيعزف الموسيقى الimmune system + +307 +00:23:13,290 --> 00:23:16,410 +بيوصفوه بهذه الطريقة انه orchestrated انه في خلايا + +308 +00:23:16,410 --> 00:23:24,430 +بتنظم عمل الخلايا الأخرى إنتاج T cell لوظيفة انها + +309 +00:23:24,430 --> 00:23:29,510 +تجيب تفرز cytokines الcytokines اتنادي فيها على + +310 +00:23:29,510 --> 00:23:33,890 +other cells cytokines specific تخيلوا كل الصف + +311 +00:23:33,890 --> 00:23:39,430 +سيطلع cytokines زي ما كل الصف بيصير صوت حد هيفهم .. + +312 +00:23:39,430 --> 00:23:44,670 +هيفهم انه جهاز المناعة كله هيجي على المكان طيب + +313 +00:23:44,670 --> 00:23:49,870 +احنا ردة الفعل اللي المطلوبة هي ردة فعل تتناسب مع + +314 +00:23:49,870 --> 00:23:55,300 +حجم الantigen لما الantigen هذا يعمل رد فعل مناعي + +315 +00:23:55,300 --> 00:23:59,600 +ضخمة بيلاقيه بسرعة بسرعة exaggerated immune + +316 +00:23:59,600 --> 00:24:04,860 +response يعني رد فعل مناعي مبالغ فيه يؤدي إلى + +317 +00:24:04,860 --> 00:24:08,540 +ماذا؟ إلى release لـ histamine وinflammatory + +318 +00:24:08,540 --> 00:24:17,080 +substances وقد تؤدي إلى shock and death تخيلوا؟ + +319 +00:24:17,080 --> 00:24:21,720 +من ايه؟ من انه ليه ليه يسموه super antigen؟ أكيد + +320 +00:24:21,720 --> 00:24:25,880 +يقولوا super يعني حاجة مميزة، means الأساسية + +321 +00:24:25,880 --> 00:24:31,240 +فقط انه بيقدر يعمل non-specific activation of T cells + +322 +00:24:52,510 --> 00:24:56,870 +أما لما يكون localized الأمر متابعة بيكون إن شاء + +323 +00:24:56,870 --> 00:24:57,330 +الله بسيط + +324 +00:25:00,690 --> 00:25:06,470 +الخلايا بيصير فيها انقسامات كمية كبيرة produced من + +325 +00:25:06,470 --> 00:25:10,450 +الcytokines لا تتناسب إطلاقا مع حجم الthreat + +326 +00:25:16,390 --> 00:25:20,310 +لاحظوا أنا لففت على مين؟ على كل الـ PC لأن كل جهاز + +327 +00:25:20,310 --> 00:25:23,670 +المناعة بيتأثر بهذا وطبعا بيصير فيه ارتفاع في + +328 +00:25:23,670 --> 00:25:27,190 +درجة الحرارة اللي هو الـ PC مدام صارنا داخلين في ال + +329 +00:25:27,190 --> 00:25:31,530 +animation نكمل ونشوف الendotoxin وذكركم احنا + +330 +00:25:31,530 --> 00:25:37,840 +نستعرض اللوحة، يا عزيزي نركز على اللوحة إن هو + +331 +00:25:37,840 --> 00:25:40,480 +عبارة عن component من الcell من الouter cell من + +332 +00:25:40,480 --> 00:25:43,080 +بنية بgram negative bacteria وكان مكون من O-sided + +333 +00:25:43,080 --> 00:25:45,840 +chain والlipid A، الlipid A هو الendotoxin + +334 +00:25:45,840 --> 00:25:50,980 +الماكروفاج بتاكل البكتيريا أو الphagocyte، جوا + +335 +00:25:50,980 --> 00:25:55,260 +الماكروفاج بيصير فيه عملية اللي هو التحليل لل .. ال + +336 +00:25:55,260 --> 00:25:59,990 +.. ال .. البكتيريا وتكسيرها بيصير release لمن؟ لل + +337 +00:25:59,990 --> 00:26:04,910 +endotoxin بيطلع ينسرخانس الوInterleukin ال + +338 +00:26:04,910 --> 00:26:08,310 +Interleukin بيروح على الhypothalamus في الدماغ + +339 +00:26:08,310 --> 00:26:11,710 +والآن الhypothalamus مسئول عن الthermal regulation + +340 +00:26:11,710 --> 00:26:17,670 +في الجسم فبترتفع درجة الحرارة أول حاجة بترتفع درجة + +341 +00:26:17,670 --> 00:26:22,570 +الحرارة طبعا في أشياء أخرى كثيرة من ضمنها العمليات + +342 +00:26:22,570 --> 00:26:24,770 +الفاجوسايتوز الكثيرة والantibodies اللي بيصير + +343 +00:26:24,770 --> 00:26:30,520 +تنتج لكن في نقطة كثيرة مهمة، وجود البكتيريا + +344 +00:26:30,520 --> 00:26:36,750 +والتوكسين بيؤدي إلى انخفاض في ضغط الدم أحد أهم + +345 +00:26:36,750 --> 00:26:40,950 +أسباب الخطورة في الendotoxin هو انخفاض اللي هو ال + +346 +00:26:40,950 --> 00:26:42,450 +blood pressure اللي هو الأصل اللي .. الأصل اللي + +347 +00:26:42,450 --> 00:26:46,630 +لازم يكون على كده؟ 120 على 80 مثلا أو 110 على 70 + +348 +00:26:46,630 --> 00:26:50,730 +بحسب الجنس ذاك على الأنثى، لاحظوا أنا مش بصير أنا + +349 +00:26:50,730 --> 00:26:55,370 +جالس، بسبب هبوط في ضغط الدم، طب لما يصل ضغط + +350 +00:26:55,370 --> 00:27:00,830 +الدم لهذه .. لهذه الدرجة، أي حركة قد تفقدنا + +351 +00:27:00,830 --> 00:27:04,710 +التوازن أو ممكن حتى نصل بنا إلى shock and death + +352 +00:27:05,210 --> 00:27:08,850 +فالendotoxin يا بنات التوكسين تبع الgram + +353 +00:27:08,850 --> 00:27:12,410 +negative bacteria اللي احنا تعلمناه في أول الفصل و + +354 +00:27:12,410 --> 00:27:16,370 +قلنا عنه جزء من الcomponent تبع كل gram negative + +355 +00:27:16,370 --> 00:27:21,990 +bacteria لو البكتيريا موجودة في جسمنا وعالجناها و + +356 +00:27:21,990 --> 00:27:28,450 +قضينا عليها هذا وحده لا يكفي لأنه قد نقتل المريض + +357 +00:27:28,450 --> 00:27:32,210 +بقتل الألمين للبكتيريا وبالتالي بدنا نكون عندنا + +358 +00:27:32,210 --> 00:27:36,390 +اطلاع أو معرفة بأن الendotoxin له effects كثيرة + +359 +00:27:36,390 --> 00:27:40,090 +من ضمنها كذا مرة low blood pressure high + +360 +00:27:40,090 --> 00:27:44,010 +temperature وفي عندنا اللي هو coma and death طب + +361 +00:27:44,010 --> 00:27:48,470 +خليني بس نوضح النقطة هذه الآن أنتم بتعرفوا مركب + +362 +00:27:48,470 --> 00:27:52,310 +الacetylcholine اسمها فيه؟ إيش هو دي بتقولها ده؟ + +363 +00:27:52,310 --> 00:27:58,520 +neurotransmitter إيش هي transmission؟Bath، bath + +364 +00:27:58,520 --> 00:28:02,560 +يعني نقل إشارة، فالneurotransmitter هو نقل عصبي، + +365 +00:28:02,560 --> 00:28:06,500 +نقل عصب أو إشارة عصبية، الbotulinum toxin حضرتوش + +366 +00:28:06,500 --> 00:28:10,760 +بيعمل .. بيعمل inhibition للأستيل كولين، يبقى + +367 +00:28:10,760 --> 00:28:14,400 +مافيش نقل للإشارة العصبية، مافيش إرسال، مافيش + +368 +00:28:14,400 --> 00:28:17,540 +إرسال .. مافيش إرسال يبقى مافيش استقبال، وده لا .. + +369 +00:28:17,540 --> 00:28:21,080 +وده مافيش استقبال يبقى فينا شلل، أي نوع من الشلل + +370 +00:28:22,710 --> 00:28:25,530 +الacetylcholine المفروض أن يعطينا إشارة + +371 +00:28:25,530 --> 00:28:28,450 +للcontraction، ما تنفيش contraction، إيش معناه + +372 +00:28:28,450 --> 00:28:32,650 +حاجة تكون فينا؟ relaxation، شلل بيسموه آخر جملة + +373 +00:28:32,650 --> 00:28:36,010 +flaccid paralysis، فالflaccid paralysis معناته + +374 +00:28:36,010 --> 00:28:40,200 +شلل ارتخائي، شلل ارتخائي وأنتم فاكرين لما تكلمنا + +375 +00:28:40,200 --> 00:28:45,020 +عن الtoxin هذا قلنا لكم بيعطوه ك .. للتجميل في ال + +376 +00:28:45,020 --> 00:28:49,760 +botox أو ما يسمى الـ botox وقلنا سبب استخدامه هو + +377 +00:28:49,760 --> 00:28:53,100 +أن التجايد هي عبارة عن .. يعني بتكون تقلصات + +378 +00:28:53,100 --> 00:28:56,300 +contracted skin أو .. أو muscles في الـ skin فهو + +379 +00:28:56,300 --> 00:28:59,080 +لما لها عملته اللي هو relaxation لكن هذا قلنا كلمة + +380 +00:28:59,080 --> 00:29:04,100 +مرة قولنا diluted بيكون في فترة مؤقتة لكن بنفهم إن + +381 +00:29:04,100 --> 00:29:06,960 +الـ mechanism of action كانت مبنية على مين؟ على + +382 +00:29:06,960 --> 00:29:12,680 +أنه فقدان الإشارة العصبية عن طريق منع إنتاج من الـ + +383 +00:29:12,680 --> 00:29:17,060 +acetylcholine أنا بقول لكم احفظوا لإنه اللي وراه على + +384 +00:29:17,060 --> 00:29:25,700 +طول اللي هو الـ tetanus عكسه تمانع inhibits + +385 +00:29:25,700 --> 00:29:30,020 +glycine release إيش مشكلة الـ glycine هذا؟ الآن + +386 +00:29:30,020 --> 00:29:34,960 +مافيش botulism فيه normal في normal contraction و + +387 +00:29:34,960 --> 00:29:38,200 +relaxation الـ contraction بينتج على أنه في + +388 +00:29:38,200 --> 00:29:43,340 +أسيتالكولين تمام؟ و الـ relaxation لجليسين بيقول ل + +389 +00:29:43,340 --> 00:29:46,720 +.. بيقول لجليسين بيقول لأسيتالكولين بكف خلاص روح + +390 +00:29:46,720 --> 00:29:50,120 +تمام؟ فبصير العملية فيها نوع من إيه؟ من الإتزان + +391 +00:29:50,120 --> 00:29:54,400 +الأسيتالكولين بيعطي الإشارة و أنا بقطع الـ + +392 +00:29:54,400 --> 00:29:59,120 +الإشارة فبصير العملية طبيعية طيب في الـ tetanus + +393 +00:29:59,120 --> 00:30:05,100 +الوضع اختلف في أسيتالكولين وعمل contraction احنا + +394 +00:30:05,100 --> 00:30:09,560 +مانعنا مين؟ مانعنا اللي بيمنع الـ acetylcholine + +395 +00:30:09,560 --> 00:30:13,800 +فصار عايش عندي contraction على طول بيبقى الشرر + +396 +00:30:13,800 --> 00:30:16,400 +اللي صار عندي شرر مختلف تماما عن الأولاني هذا + +397 +00:30:16,400 --> 00:30:19,860 +الأولاني إيش كان؟ كان relaxed أو سبب لها flaccid + +398 +00:30:19,860 --> 00:30:24,460 +paralysis هذا بيسموه spastic، spastic يعني انقباضي + +399 +00:30:24,460 --> 00:30:29,580 +وعشان قلت لكم أنه الـ spasm أو ال .. ال .. ال .. + +400 +00:30:30,210 --> 00:30:34,730 +التقلصات أو التشهنجات اللي تنتج عند مرضى التتانوس + +401 +00:30:34,730 --> 00:30:40,110 +قد تكون شديدة وتكسر العمود اللي هو Alpha ال + +402 +00:30:40,110 --> 00:30:42,850 +animation اللي قدامنا هذا على فكرة واضحة برضه + +403 +00:30:42,850 --> 00:30:48,850 +بتعطينا فكرة على اللي هو العملية بتاعة الـ + +404 +00:30:48,850 --> 00:30:54,310 +الـ الأولفي عندي هناش الـ acetylcholine receptor و هذا + +405 +00:30:54,310 --> 00:30:58,810 +الـ glycine اللي هو الـ glycine لإن الـ toxin بيرفبط + +406 +00:30:58,810 --> 00:31:02,210 +بي هذا المنطقة و بيمنع مين؟ بيمنع الـ glycine + +407 +00:31:02,210 --> 00:31:06,430 +فبالتالي بيصير فيه إنتاج مستمر للـ acetylcholine و + +408 +00:31:06,430 --> 00:31:09,490 +action مستمر للـ acetylcholine و بالتالي مافيش أنها + +409 +00:31:09,490 --> 00:31:14,830 +إن كان هي لأ فإنها contraction مستمر و لا يوجد + +410 +00:31:14,830 --> 00:31:20,090 +relaxation وهذا ال .. ال .. مريض .. مريض تتنوس بي + +411 +00:31:20,090 --> 00:31:25,670 +.. يعني بيمر براحة كتيرة من ضمنها اللي هو ال + +412 +00:31:25,670 --> 00:31:29,330 +paralysis لعضلات الفك لذلك باللي جيه بالعربي + +413 +00:31:29,330 --> 00:31:33,670 +بيسموه الكزاز ليش؟ لإنه كز ع أصنانه لإن عضلات .. + +414 +00:31:33,670 --> 00:31:37,890 +أو بيسموه بالإنجليزي fixed smile الإبتسامة الثابتة + +415 +00:31:37,890 --> 00:31:41,330 +دايما المريض بيكون مبتسم وعشانك مش كل واحد بيبتسم + +416 +00:31:41,330 --> 00:31:44,450 +أنه ممكن .. يعني يكون مبتسم أنه سعيد ربما يكون + +417 +00:31:44,450 --> 00:31:52,620 +عنده تتنوس طيب الـ X-toxin في ال .. ال .. في ال + +418 +00:31:52,620 --> 00:31:56,000 +extra cellular matrix of connective tissue مثال + +419 +00:31:56,000 --> 00:32:01,540 +احنا درسنا في الـ invasions تمام بعض الـ toxins + +420 +00:32:01,540 --> 00:32:05,140 +الكرستوديوم بقى رفرنجز على سبيل المثال بتنتج + +421 +00:32:05,140 --> 00:32:07,240 +collagenase مثلا الـ collagenase درسناها في وين؟ + +422 +00:32:07,240 --> 00:32:10,780 +كان .. اه في الـ invasion في الـ invasion حاكيناه + +423 +00:32:10,780 --> 00:32:15,060 +الآن و حاكيناه و اسمه واضح إن هو عبارة عن إيه؟ عن + +424 +00:32:15,060 --> 00:32:20,700 +انزين بولوجينايز بيكسر لكن كونه كونه بيقتل الخلايا + +425 +00:32:20,700 --> 00:32:23,580 +نتيجة نتيجة إن هي بتقتل الخلايا فممكن نعتبره برضه + +426 +00:32:23,580 --> 00:32:28,100 +إيه؟ ممكن نعتبره toxins يعني enzymes with toxic + +427 +00:32:28,100 --> 00:32:32,640 +activity الاستبتاكوكاس حياليرونيديز و بيكسر + +428 +00:32:32,640 --> 00:32:35,220 +الهياليرونيك اسد اللي برضه بلبط الخلايا ببعضها + +429 +00:32:35,220 --> 00:32:42,670 +البعض membrane-damaging-toxins شوفناهم في الـ + +430 +00:32:42,670 --> 00:32:45,650 +animation مش بيقولنا احنا إيه بيو موديل و membrane + +431 +00:32:45,650 --> 00:32:49,710 +و بعدين بيقولنا super-antigens زي الـ proteases + +432 +00:32:49,710 --> 00:32:54,970 +كلمة الـ proteases هي عنوان لما احنا بيقوله برهات + +433 +00:32:54,970 --> 00:33:00,630 +فلوس املة بيتكلم عن دينار املة و دولار املة و شكل + +434 +00:33:00,630 --> 00:33:05,800 +املة و درهم املة فلكن في الآخر فيه لهم عنوان رئيسي + +435 +00:33:05,800 --> 00:33:09,000 +لما بيقول الـ proteases هي كل الإنزيمات القادرة على + +436 +00:33:09,000 --> 00:33:12,180 +تكسير الـ polypeptide أو الـ proteinات فاهمين؟ في + +437 +00:33:12,180 --> 00:33:15,580 +منها أصناف متخصصة بتكون في منها أصناف متخصصة + +438 +00:33:15,580 --> 00:33:17,960 +phospholipases و أنا حاطط بين قوسين اللي هو ال + +439 +00:33:17,960 --> 00:33:21,640 +acetinase اللي قولنا عنه واحد من الـ phospholipases + +440 +00:33:21,640 --> 00:33:25,540 +وفي منهم detergent like action إيش يعني ال + +441 +00:33:25,540 --> 00:33:29,700 +detergent like action؟ احكي + +442 +00:33:37,270 --> 00:33:39,130 +وهو دي أماق يقولي احنا هنقولي اشتغلوا كال .. + +443 +00:33:39,130 --> 00:33:44,690 +كالمناطق تانية، نعم، بس كيف المناطق بيشتغل؟ الشكل + +444 +00:33:44,690 --> 00:33:49,610 +هو بدنا quiz، بدنا quiz إيش، ديك مفاجئ، عشان يحفظ + +445 +00:33:49,610 --> 00:33:51,630 +ال .. ال .. الذاكرة، عموما + +446 +00:33:55,680 --> 00:33:59,420 +كان فيه إله Hydrophilic و Hydrophobic و الـ cell + +447 +00:33:59,420 --> 00:34:01,720 +membrane تبعنا الـ phospholipids تبعتنا كانت + +448 +00:34:01,720 --> 00:34:04,300 +Hydrophilic و Hydrophobic الـ interaction between + +449 +00:34:04,300 --> 00:34:07,280 +the two بيعمل destruction لمين؟ للـ membrane نفسه ما هو + +450 +00:34:07,280 --> 00:34:13,880 +الـ detergent like action طيب ناخد واحد من الأمثلة + +451 +00:34:13,880 --> 00:34:16,060 +اللي هو الـ Clostridium perfringens + +452 +00:34:16,060 --> 00:34:19,500 +phospholipids بالمناسبة الـ Clostridium + +453 +00:34:19,500 --> 00:34:23,830 +perfringens مررت معانا قبل هيك تحت أي عنوان أو + +454 +00:34:23,830 --> 00:34:32,030 +تحت أي فكرة أو تحت أي مرض مين بتذكر؟ + +455 +00:34:32,030 --> 00:34:34,350 +خدوا بأكوا مبررات الـ quiz و قولوا مانا قاعد بالزيد + +456 +00:34:34,350 --> 00:34:37,370 +الفوسفول + +457 +00:34:37,370 --> 00:34:40,230 +.. هقولوا .. هقولوا مول مرة معانا .. لما اتكلمنا + +458 +00:34:40,230 --> 00:34:43,290 +عن الـ endospore اتكلمنا عن الـ endospore اتكلمنا + +459 +00:34:43,290 --> 00:34:48,410 +على two Genera, Bacillus و Clostridium و مضمن الـ + +460 +00:34:48,410 --> 00:34:52,610 +Clostridium تحدث عن ثلاث أصناف Perfringens و + +461 +00:34:52,610 --> 00:34:56,450 +Botulinum و Tetanus وقلنا لكم الـ Perfringens هي + +462 +00:34:56,450 --> 00:35:02,190 +المسؤولة عن الجاز Gangrene الغرينة الغازية و أشهر + +463 +00:35:02,190 --> 00:35:08,170 +توكسين عندها هو الـ phospholipase C اللي من خلالها + +464 +00:35:08,170 --> 00:35:14,080 +بتفكك الخلايا عن بعضها أو تقتل الخلايا طبعا خدوا + +465 +00:35:14,080 --> 00:35:18,480 +بالك يا بنات قتل الخلايا بيخدم غرضين إنه .. إنه .. + +466 +00:35:18,480 --> 00:35:21,840 +أنه .. ال .. ال .. ال .. الخلايا اللي ماتت هتشكل + +467 +00:35:21,840 --> 00:35:27,280 +إيش لان؟ مادة غذائية مناسبة للبكتيريا، أنتوا عارفين + +468 +00:35:27,280 --> 00:35:31,020 +على إيش بنربي احنا و بنمي الكلسترديوم؟ بنميها على + +469 +00:35:31,020 --> 00:35:35,940 +قطع اللحم، بنجبطيلها لحم تمام شجرة صغيرة و + +470 +00:35:35,940 --> 00:35:40,790 +بنغليها، اسم الـ media is chopped meat broth بتحب + +471 +00:35:40,790 --> 00:35:43,710 +البروتينات بشكل كبير و اللحم مليان بروتينات تمام؟ + +472 +00:35:43,710 --> 00:35:46,790 +اللحوم فلما اتنوت خلايا العضلات بتاعتها اللي هي + +473 +00:35:46,790 --> 00:35:50,310 +اللحم تمام هي في واقع الأمر ما بتصيب غرضين، الغرض + +474 +00:35:50,310 --> 00:35:55,310 +الأول إنه بتعمل invasion، رقم اتنين بتحصل عليها + +475 +00:35:55,310 --> 00:35:59,570 +مواد مجدية من الخلايا اللي ماتت، الـ phospholipase + +476 +00:35:59,570 --> 00:36:02,750 +له multiple functions، it destroys blood + +477 +00:36:06,040 --> 00:36:09,380 +Stops influx of inflammatory cells عنده إمكانية + +478 +00:36:09,380 --> 00:36:14,960 +يقول للخلايا اللي جاية تعمل رد فعل مناعي، يقولها + +479 +00:36:14,960 --> 00:36:20,200 +إنك واقفي، ما تمشي هام It helps، أنا غلطان في الجملة + +480 +00:36:20,200 --> 00:36:23,140 +هنا، it helps in creating an anaerobic environment + +481 +00:36:23,140 --> 00:36:28,940 +بيساعد في خلق بيئة لا هوائية، لأن البكتيريا أجمل + +482 +00:36:28,940 --> 00:36:33,040 +هذه البكتيريا لا هوائية، وبالتالي هي محتاجة إلى هذه + +483 +00:36:33,040 --> 00:36:36,220 +البيئة allows growth of this anaerobic طبعا + +484 +00:36:36,220 --> 00:36:38,860 +creating anaerobic environment بيساعد على إن + +485 +00:36:38,860 --> 00:36:45,180 +البكتيريا تنمو في هذه البيئة اللهوة طيب + +486 +00:36:45,180 --> 00:36:50,420 +مازلنا في الـ exotoxin و متكلم على .. ركزوا معايا لو + +487 +00:36:50,420 --> 00:36:55,700 +واحد أكل طعام بيحتوي على الـ botulinum toxin إيش + +488 +00:36:55,700 --> 00:37:01,870 +حله؟ إيش علاجه؟ إن نعطيه antitoxin زي واحد جرس طوحى + +489 +00:37:01,870 --> 00:37:08,030 +أو عقرب نعطيه antitoxin الـ antitoxin شو منه ده؟ + +490 +00:37:08,030 --> 00:37:12,330 +عبارة عن antibodies بسموها + +491 +00:37:12,330 --> 00:37:17,830 +neutralizing antibody ليش سموها neutralizing؟ + +492 +00:37:20,660 --> 00:37:22,920 +فالآن الـ antibodies أما بيقولون عنها antibodies + +493 +00:37:22,920 --> 00:37:26,920 +عادية أو بيقولون عنها antitoxins يعني مثلا لما + +494 +00:37:26,920 --> 00:37:28,740 +بيقولوا Clostridium botulinum antitoxin + +495 +00:37:28,740 --> 00:37:33,120 +الـ Clostridium tetani antitoxin يبقى لما يكون الـ + +496 +00:37:33,120 --> 00:37:39,060 +antibody مخصص للتوكسين بنسميه antitoxin و غالبا الـ + +497 +00:37:39,060 --> 00:37:46,200 +exotoxin بنقدر نتجلها antibody الاندوتوكسين بيكوّل + +498 +00:37:46,200 --> 00:37:49,700 +لها انتبالي لكن مش effective .. مش as effective as + +499 +00:37:49,700 --> 00:37:54,360 +اللي هو الـ antitoxin لمين؟ للإكسودوكسين بيعطيكم + +500 +00:37:54,360 --> 00:37:59,840 +أمثلة كثيرة الـ ديثيريا الفاكسين اللي احنا بناخده + +501 +00:37:59,840 --> 00:38:03,000 +.. بناخده .. بيعطيكوا اسم الفاكسين اللي بتحفظه .. + +502 +00:38:03,000 --> 00:38:06,080 +أنا بكتبه على الصبورة عشان نضيفه من ضمن ال .. + +503 +00:38:18,580 --> 00:38:28,420 +دفتيريا، هاي دفتيريا، و هاي تيتانوس، و بيرتوز + +504 +00:38:28,420 --> 00:38:28,940 +بالأول + +505 +00:38:49,600 --> 00:38:53,280 +تمام؟ هذا التوكسين اللي هو الـ DPT هو مشهور كل جوه + +506 +00:38:53,280 --> 00:38:57,420 +أخدته تمام؟ بيحمينا من الـ Diphtheria و من الـ + +507 +00:38:57,420 --> 00:39:00,340 +Pertussis اللي يساعدكي زي اللي هو مين اللي هو + +508 +00:39:00,340 --> 00:39:04,140 +الثلاثة بيشتركوا بإنهم ينتجوا بينتجوا + +509 +00:39:04,140 --> 00:39:08,520 +Exotoxin و بناخد الـ Exotoxin تبعهم و بنحوله لما + +510 +00:39:08,520 --> 00:39:13,480 +يسمى إلى Toxoid ماربعها المصطلحات العجب لها؟ لأ، + +511 +00:39:13,480 --> 00:39:21,340 +توكسين بتحول لإيه؟ Toxoid بنقول Oidإيش معناها؟ + +512 +00:39:21,340 --> 00:39:29,120 +Oid، metal، metalloid، alkaline، alkaloid، شبيه + +513 +00:39:29,120 --> 00:39:33,480 +بيه، شبيه بيه، metal، metalloid، إيش بقى؟ Toxin، +514 +00:39:33,480 --> 00:39:35,700 +Toxoid، خليكم معايا برنامج تنفذوا معايا في الجزئية + +515 +00:39:35,700 --> 00:39:42,230 +هذه إذا هالتوكسين إيه له صفتين بدنا نركز عليهم صفة + +516 +00:39:42,230 --> 00:39:46,590 +أولى الـ Toxicgenicity إيه الـ Toxicitygenicity؟ + +517 +00:39:46,590 --> 00:39:53,410 +السمية والصفة الثانية الـ Antigenicity أنه لو دخل + +518 +00:39:53,410 --> 00:39:57,170 +جسم الإنسان جسم الإنسان بيكوّله أجسام مضادة احنا + +519 +00:39:57,170 --> 00:40:02,450 +مين اللي بيدينا؟ الـ Toxicgenic فبنروح و احنا .. + +520 +00:40:02,450 --> 00:40:09,020 +يعني لو نعملها بشكل خياليالطرفين هذا الـ Toxic part + +521 +00:40:09,020 --> 00:40:14,400 +و هذا إيش قلناه اللي هو الـ Antigenic part لو جسينا + +522 +00:40:14,400 --> 00:40:19,420 +الـ Toxigenic و خلينا بس الـ Antigenic ودخلنا هذا الـ + +523 +00:40:19,420 --> 00:40:23,340 +Antigen أو هذا الـ Toxin إلى جسم الحيوان، جسم + +524 +00:40:23,340 --> 00:40:27,140 +الحيوان بيكوّله أجسام مضاد دون أن يتأثر أن الـ + +525 +00:40:27,140 --> 00:40:31,730 +Toxigen استعمالها راحبناخد الـ Antibodies الجاهزة + +526 +00:40:31,730 --> 00:40:36,330 +هذه و بتصير أنها مصدر لو انزلجنا بدنا نعطي مريض + +527 +00:40:36,330 --> 00:40:40,750 +Antibodies نعطيه إيه؟ أمان؟ طيب احنا بدنا نضلنا + +528 +00:40:40,750 --> 00:40:43,330 +نصنع فيه الحيوانات و الحيوانات بدنا نصفي الـ + +529 +00:40:43,330 --> 00:40:46,810 +Antibodies و قد يبقى بقايا لو حقنها في إنسان كونه + +530 +00:40:46,810 --> 00:40:50,830 +غريب ممكن الـ Serum تبقى الحيوان يؤذي مين؟ الإنسان + +531 +00:40:50,830 --> 00:40:57,360 +فلماذا نقوم بإعطاء المريض نفسه تطعيم محتوي علىالـ + +532 +00:40:57,360 --> 00:41:01,460 +.. على الـToxoid اللي احنا هو يبارح عن تكسين جسنا + +533 +00:41:01,460 --> 00:41:05,680 +منه أو خربنا فيه اللي هو الـ Toxicgenic part دون + +534 +00:41:05,680 --> 00:41:09,720 +أنه أثر على مين؟ على الـ Antigenic part وسط الصورة + +535 +00:41:09,720 --> 00:41:13,840 +هاي الـ Toxoid، الـ Toxoid يبارح عن تكسين تم تدمير + +536 +00:41:13,840 --> 00:41:18,660 +الـ Toxicgenic side تبعه وهذا هو اللي أنتو أخدتوا + +537 +00:41:18,660 --> 00:41:21,440 +.. لما أخدتوا الـ Diphtheria و الـ Pertussis و إيه؟ + +538 +00:41:21,440 --> 00:41:27,340 +الـ تيتانوس اللي هو الـ DPT إذا بنقدر نستنتج من اللوحة + +539 +00:41:27,340 --> 00:41:30,860 +دي اللي بدأنا نتكلم عنها استنتاج مهم جدا أن الـ + +540 +00:41:30,860 --> 00:41:35,740 +Exotoxins يمكن الـ Neutralization تبعتها بمين ب .. + +541 +00:41:35,740 --> 00:41:41,160 +ب .. بواسطة الـ Antitoxin هو يمكن لو أنا اتناولت + +542 +00:41:41,160 --> 00:41:45,240 +الغذاء أنا عندي توكسين الآن دخل مش بده يجيني بعد + +543 +00:41:45,240 --> 00:41:48,540 +سنة أو سنتين الآن دخل فعشان أنا عاجز أني أعالجه ب + +544 +00:41:48,540 --> 00:41:49,580 +Antitoxin + +545 +00:42:09,380 --> 00:42:13,880 +الشخص اللي أكل الطعام من فاسد هيكوسيرتوكسين، + +546 +00:42:13,880 --> 00:42:17,600 +فالناس جاهزة منها على هذا، يعني لإنه تلقائي بدون + +547 +00:42:17,600 --> 00:42:23,420 +إشكالجهاز المناعة إذا كان بيقدر يتعامل مع + +548 +00:42:23,420 --> 00:42:27,980 +المركبات بشكل عام بيختلف، يعني أنت لاحظ شغلة، في + +549 +00:42:27,980 --> 00:42:31,620 +سموم أقوى من سموم، مظبوط ولا لأ؟ لماذا سموم أقوى + +550 +00:42:31,620 --> 00:42:34,640 +من سموم؟ في سموم تشتغل على النار على طول وانتهى من + +551 +00:42:34,640 --> 00:42:39,920 +الموضوع، جهاز المناعة بالحاجش حتى يفكر، طبعا؟ وفي + +552 +00:42:39,920 --> 00:42:44,080 +Toxins بتكون بطيئة ممكن تنبه جهاز المناعة + +553 +00:42:44,080 --> 00:42:48,600 +وبالتالي يكون في Action لها، فأنا بعطي شغل تاني + +554 +00:42:49,470 --> 00:42:53,130 +بعطي Antitoxin جاهز برا + +555 +00:42:53,130 --> 00:42:58,970 +أو بعطي Vaccine .. Vaccine لمين؟ تخيل واحد زاكيتنا + +556 +00:42:58,970 --> 00:43:04,430 +ماعرفش أهتجاج في ال .. في خدنا نقول في العمل في + +557 +00:43:04,430 --> 00:43:07,710 +الميدان، مسامير وغيره وغيرته، يعني أقل أو أفضل من + +558 +00:43:07,710 --> 00:43:12,370 +الآخر، لكن في حياته معرض أن ينصف مصمار أو مصمرين + +559 +00:43:12,370 --> 00:43:18,220 +ولا أداة جرح من هنا أو من هناك، إيش بناعطيه؟ نعطيه + +560 +00:43:18,220 --> 00:43:22,600 +Toxoid اللي هو Toxin مضعف، فجسمنا بيكون كوّن أجسام + +561 +00:43:22,600 --> 00:43:27,760 +مضادة، فيما لو دخل الـ Toxin حقا، جسمنا يقوم + +562 +00:43:27,760 --> 00:43:31,980 +بإنقاذه، بس لو حصل و أخدنا Toxin التكسين، + +563 +00:43:31,980 --> 00:43:35,600 +تناولنا، تمام؟ مافيش لنا حل إلا إنه ناخد Antitoxin + +564 +00:43:35,600 --> 00:43:36,360 +Toxoid + +565 +00:43:44,230 --> 00:43:48,350 +نُجلِع اللوحة المتعلقة بالـ Endotoxin و نطلع على + +566 +00:43:48,350 --> 00:43:54,810 +الرسم اللي موجودة هيصرف معروضة أمامكم بأشكال و + +567 +00:43:54,810 --> 00:43:58,850 +ألوان مختلفة منذ أن بدأنا بالحديث عن الـ Endotoxin + +568 +00:43:58,850 --> 00:44:03,010 +في النهاية هي مكوّنة من ثلاثة أجزاء Lipid-A, + +569 +00:44:03,350 --> 00:44:09,050 +Corepolysaccharide, and O-antigen chain الـ Endotoxin + +570 +00:44:09,050 --> 00:44:11,910 +هو الـ Lipid-A مش مانع الحاجة .. مش مانع الحاجة أن + +571 +00:44:11,910 --> 00:44:15,510 +هو الأهم، بس احنا لإننا بنتكلم عن الـ Toxins تمام، + +572 +00:44:15,510 --> 00:44:24,750 +بنتكلم عن الـ Lipid A، الـ Lipid A اللي + +573 +00:44:24,750 --> 00:44:30,530 +سميناه كامرة للـ Endotoxin طبعا عشان يصيرله Release + +574 +00:44:30,530 --> 00:44:34,410 +الـ Cell wall لازم يصيرله تحلل If it is required + +575 +00:44:34,410 --> 00:44:38,910 +for its release that the cell wall is lysed ممكن + +576 +00:44:38,910 --> 00:44:42,450 +نوصفه هذا ال .. ال .. ال .. الـ Library و ال .. أنه + +577 +00:44:42,450 --> 00:44:46,490 +Formaldehyde and heat resistant يعني فينا نوع من + +578 +00:44:46,490 --> 00:44:50,110 +إيه؟ من مقاومة للـ Formaldehyde ليش الـ Formaldehyde + +579 +00:44:50,110 --> 00:44:53,170 +بالذات؟ لأن الـ Formaldehyde هو اللي استخدمناه يا + +580 +00:44:53,170 --> 00:44:58,930 +بنات لتحويل الـ Toxin .. الـ Exotoxin لـ Toxoid يبقى + +581 +00:44:58,930 --> 00:45:04,850 +الـ Exotoxin حولت إلى Toxoid بواسطة مين يبقى الـ + +582 +00:45:04,850 --> 00:45:08,190 +Formaldehyde عمل Destruction للـ Toxic side لكن + +583 +00:45:08,190 --> 00:45:11,390 +للأسف لما حطيناها على الـ Endoxin ما قدرنا .. كنا + +584 +00:45:11,390 --> 00:45:15,590 +بننقل نفس الفكرة أننا نعمل Toxoid من الـ Endoxin + +585 +00:45:15,590 --> 00:45:20,050 +ما قدرنا ما قدرناش تمام؟ فإن الـ Formaldehyde and heat + +586 +00:45:20,050 --> 00:45:23,550 +resistant poor antigen يعني و هذا كمان أحد نقاط + +587 +00:45:23,550 --> 00:45:29,150 +قوته و نقاط ضعفه نقاط قوة للبكتيريا أن الجسم لا + +588 +00:45:29,150 --> 00:45:33,690 +بيكوّله أجسام مضادة بشكل قوي وبتالي ما بتقدرش .. + +589 +00:45:33,690 --> 00:45:38,210 +ما بتقدرش نقاوم البكتيريا من خلالها As a free + +590 +00:45:38,210 --> 00:45:41,790 +molecule هذا اليوم ما عرفش يعني .. المرة أنا ممكن + +591 +00:45:41,790 --> 00:45:44,650 +أبسلها و لا .. لو .. لو .. لو .. لو رابطنا .. لو + +592 +00:45:44,650 --> 00:45:49,270 +رابطنا ب .. بحدة ثانية يعني خلّيكم معاه، هي + +593 +00:45:49,270 --> 00:45:53,130 +المركب اللي هو الـ Endotoxin، كـ Free molecule .. كـ + +594 +00:45:53,130 --> 00:45:57,410 +Free molecule، إيش فيه أنه فيه؟ فيه أنه Poor + +595 +00:45:57,410 --> 00:46:00,630 +Antigenicity، لكن أنا بنعت فيه في القلم المناعي + +596 +00:46:00,630 --> 00:46:04,430 +اسمه Adjuvant، Adjuvant، لو ركّبنا هذا ال .. الـ + +597 +00:46:04,430 --> 00:46:07,550 +molecule على الـ Adjuvant، بصير فيه نوع من ال .. + +598 +00:46:07,550 --> 00:46:11,210 +إيه؟ من الـ Response الأفضل، لكن لن يصل بأي حال من + +599 +00:46:11,210 --> 00:46:14,990 +الأحوال للـ Response تبع مين؟ تبع الـ Exotoxins اللي + +600 +00:46:14,990 --> 00:46:18,380 +شوفناها في الـ Toxoids نكمل إن شاء الله المرة القادمة diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..f41eb76d5bf019132ea508a53248f49246f1d212 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4_postprocess.srt @@ -0,0 +1,2400 @@ +1 +00:00:20,650 --> 00:00:24,270 +بسم الله الرحمن الرحيم نكمل موضوع ال path genesis + +2 +00:00:24,270 --> 00:00:29,570 +للبكتيريا و اليوم هتكلم عن جزء مهم جدا المتعلق بال + +3 +00:00:29,570 --> 00:00:33,550 +invasion هذا الجزء يأتي بعد ال virulence ال factor + +4 +00:00:33,550 --> 00:00:36,010 +الأساسي اللي درسناه اللي هو ال attachment أو ال + +5 +00:00:36,010 --> 00:00:41,070 +adherence و درسنا مجموعة من العوامل تحت هذا ال + +6 +00:00:41,070 --> 00:00:43,470 +factor و اليوم هتكلم ان شاء الله عن مجموعة من ال + +7 +00:00:43,470 --> 00:00:46,890 +factors اللي بتساهم في ال invasion للبكتيريا + +8 +00:00:48,480 --> 00:00:52,320 +أنا ذكرت مثالين الماضية في نهاية المحاضرة مثال + +9 +00:00:52,320 --> 00:00:57,620 +السلمون اللي بتاعي فيه وكنا بتدخل عبر الأمعاء عبر + +10 +00:00:57,620 --> 00:01:01,660 +الفم إلى الأمعاء و بتختلق جدار الأمعاء عبر ال + +11 +00:01:01,660 --> 00:01:06,560 +lymph nodes و بتروح على الدم و بتنتشر من الدم إلى + +12 +00:01:06,560 --> 00:01:10,680 +organs متنوعة و مختلفة و سميناها invasive + +13 +00:01:10,680 --> 00:01:15,300 +microorganism بينما تحدثنا بالمثال مقابل التيتانوس + +14 +00:01:15,300 --> 00:01:19,850 +و كنا تبقىفي نفس المكان اللي .. اللي .. اللي عملت + +15 +00:01:19,850 --> 00:01:22,770 +فيه infection بينما ال toxin تبعها هو اللي بينتقل + +16 +00:01:22,770 --> 00:01:29,190 +الى اماكن متنوعة فالان احنا هنتطرق لبعض العوامل و + +17 +00:01:29,190 --> 00:01:33,470 +اعتقد انه طرقنا اليها بمرور سريع نحاول المرايا مرة + +18 +00:01:33,470 --> 00:01:37,850 +أخرى قبل ما نبدأ في محاضرتنا ان شاء اللهلجينا ان + +19 +00:01:37,850 --> 00:01:42,670 +في مجموعة كبيرة من ال invasive factors هم عبارة عن + +20 +00:01:42,670 --> 00:01:48,870 +انزايم زي ال helionidase زي ال collagenase زي ال + +21 +00:01:48,870 --> 00:01:53,410 +coagulase زي اللي هو ال .. ال .. ال kinases و ال + +22 +00:01:53,410 --> 00:01:58,160 +neuraminidasesو ال phospholipases و ال licitinases + +23 +00:01:58,160 --> 00:02:03,120 +مظبوط ان هما ليسوا عبارة عن انزايم و هذه الانزايم + +24 +00:02:03,120 --> 00:02:07,340 +لها خطوة محددة او specific step تملة مثلا ال + +25 +00:02:07,340 --> 00:02:09,740 +hyaluronidase بيفك ال hyaluronic acid و ال + +26 +00:02:09,740 --> 00:02:12,560 +hyaluronic acid هو اللي مسئول عن ربط ال connective + +27 +00:02:12,560 --> 00:02:17,740 +tissue البعض البعض ال collagenases اللي برضه + +28 +00:02:17,740 --> 00:02:21,160 +بتفكفك الكولاجين اللي برضه بربط ال muscles ببعضهم + +29 +00:02:21,160 --> 00:02:25,150 +ببعضلما نفكر في خلية عن بعضها البعض، إيه اللي + +30 +00:02:25,150 --> 00:02:30,590 +بيصير؟ مثلا عندي فقدان .. مثلا عندي فقدان للارتباط + +31 +00:02:30,590 --> 00:02:33,390 +بين خلية وخلية وبالتالي بيصير في عندي ال block + +32 +00:02:33,390 --> 00:02:38,490 +supply لهذه الخلاية بينقطع وبالتالي في عندي death + +33 +00:02:38,490 --> 00:02:43,140 +لهذه الخلايةالكواريو ليه اصفقنا انه بيعمل ال + +34 +00:02:43,140 --> 00:02:47,660 +fibrinogen الى ال fibrin واللي هي ال clot و هال ال + +35 +00:02:47,660 --> 00:02:51,500 +clot ممكن تتجمع في أماكن مختلفة في الجسم و تم ال + +36 +00:02:51,500 --> 00:02:55,200 +plugging اغلاق لبعض ال capillaries او ال veins حتى + +37 +00:02:55,200 --> 00:02:59,820 +وبالتالي تقبل blood supply على areas على مناطق + +38 +00:02:59,820 --> 00:03:06,380 +كاملةLoss of blood supply اليوكوسايدين + +39 +00:03:06,380 --> 00:03:10,460 +والهيموليسين متخصصات في قتل نوع معين من الخلايا + +40 +00:03:10,460 --> 00:03:14,200 +النوع الأول اللي هو اليوكوسايدين ويوكوسايدس + +41 +00:03:14,200 --> 00:03:17,140 +والتانية اللي هي الارتراوسايدس كلمة هيموليسين تما + +42 +00:03:17,140 --> 00:03:23,220 +الهيموليسز لل red blood cellsواخرش هدفنا عن نوعين + +43 +00:03:23,220 --> 00:03:28,600 +من التوكسين حد اسمها anthrax elongation factor افن + +44 +00:03:28,600 --> 00:03:32,960 +edema factor والتاني pertosis adenylate cyclase + +45 +00:03:32,960 --> 00:03:36,320 +وكانوا التانين بيشتغلوا بنفس القدرة التانين + +46 +00:03:36,320 --> 00:03:40,540 +بيعملوا كتالة يا بنات بيعملوا activation لأنزيم + +47 +00:03:40,540 --> 00:03:46,060 +اسمه adenylate cyclase enzyme هذا الانزيم بيحول ال + +48 +00:03:46,060 --> 00:03:52,370 +ATP إلى cyclic AMPالـ Cyclic AMP بيعمل عملية + +49 +00:03:52,370 --> 00:03:56,130 +increase لل production of electrolytes .. مهم + +50 +00:03:56,130 --> 00:04:00,530 +بيعمل ال production هذا extra cellularly .. إيش + +51 +00:04:00,530 --> 00:04:04,030 +يعني extra cellularly؟ يعني خارج الخلية .. لأن لما + +52 +00:04:04,030 --> 00:04:08,210 +يصير فيه تركيز عالية من ال electrolytes خارج + +53 +00:04:08,210 --> 00:04:13,790 +الخلية، الدسم يميل إلى تخفيف .. إلى تخفيف هذه ال + +54 +00:04:13,790 --> 00:04:16,790 +electrolytes عبر إنتاج كمية كبيرة من مين؟ من + +55 +00:04:16,790 --> 00:04:22,360 +الساقلإذا كان ال electrolyte موجودة في تجويف ال + +56 +00:04:22,360 --> 00:04:26,760 +human زي مثلا ال intestine، بينتج عندي ديارية لأنه + +57 +00:04:26,760 --> 00:04:30,980 +سائل كمية كبيرة، إذا كان مالوش تجويف، بيصير عندي + +58 +00:04:30,980 --> 00:04:34,140 +إدمة أو الاستثقاء، اللي هي الانتفاخ الناتجة عن + +59 +00:04:34,140 --> 00:04:40,420 +وجود كمية كبيرة من مين؟ من الانتفاخ الثالث + +60 +00:04:40,420 --> 00:04:44,240 +من ال violence factors، اللي هو من التكسيفأحنا + +61 +00:04:44,240 --> 00:04:48,740 +قررنا نعامل صنفين based on the mechanism of + +62 +00:04:48,740 --> 00:04:52,260 +production endotoxins are integral part of the + +63 +00:04:52,260 --> 00:04:56,700 +cell component يعني الجزء الأساسي و لا تتحلل ولا + +64 +00:04:56,700 --> 00:05:01,300 +تطلق إلا بعد موت الخلية بينما ال exotoxins are + +65 +00:05:01,300 --> 00:05:04,900 +produced while the cells are alive عندما تكون + +66 +00:05:04,900 --> 00:05:12,380 +الخلية حية فحية و بتنتجطيب تخيلوا الأن أنها أنتجت + +67 +00:05:12,380 --> 00:05:18,280 +التوكسين تبعها في غذائنا غير علّما تنتجه داخل + +68 +00:05:18,280 --> 00:05:23,420 +أجسامنا و بعض التوكسين قد تنتج في داخل أجسامنا لكن + +69 +00:05:23,420 --> 00:05:27,240 +اتضال التأثير تبعها محصول في مكان محدد طبعا + +70 +00:05:27,240 --> 00:05:31,640 +المصطلحات اللي على السطورة produced in vitro و in + +71 +00:05:31,640 --> 00:05:33,820 +vivo بعرف المصطلحات هذي كم ولا انكم ولا لأ + +72 +00:05:33,820 --> 00:05:41,970 +انترجمهم in vivo يعنيإيش يعني؟ in test tube يعني + +73 +00:05:41,970 --> 00:05:49,190 +ليست في كائن حي معمليًا in vivo داخل الكائن الحي + +74 +00:05:49,190 --> 00:05:52,750 +فلما بنعمل experiment احنا بنسميها experiment إما + +75 +00:05:52,750 --> 00:05:55,970 +بنقول in vivo أو in vitro حسب وين إحنا إجرينا ال + +76 +00:05:55,970 --> 00:05:59,310 +experiment إذا عملناها في ال test tube عملناها في + +77 +00:05:59,310 --> 00:06:03,490 +ال petri dish دماغ بنقول عنها in vitro إذا عملناها + +78 +00:06:03,490 --> 00:06:09,010 +في الإنسان أو الحيوان بنسميها in vivoفإذا الـ + +79 +00:06:09,010 --> 00:06:12,730 +toxin produced in vivo ممكن يسبب food poisoning و + +80 +00:06:12,730 --> 00:06:15,230 +من أشهر الأمثلة ال botulinum toxin أو ال botulism + +81 +00:06:15,230 --> 00:06:19,690 +toxin و الاستفيلكوكال enterotoxin كلمة enterotoxin + +82 +00:06:19,690 --> 00:06:24,830 +الآن صلحنا مصطلح جديد كنا مصنفين exo و endو الآن + +83 +00:06:24,830 --> 00:06:28,210 +بكلمة عن entero و ال entero عبارة عن تصنيف لل + +84 +00:06:28,210 --> 00:06:32,530 +location where the toxin acts when it is some + +85 +00:06:35,740 --> 00:06:40,280 +بيشتر ال central nervous system بنسميه neurotoxin، + +86 +00:06:40,280 --> 00:06:46,400 +حسب مكان العمل، حسب مكان العمل، طب إذا بقتل كل + +87 +00:06:46,400 --> 00:06:50,180 +الخلايا، بنسميه cytotoxin + +88 +00:06:53,540 --> 00:06:58,360 +أو مصطلحات nephrotoxic يعني سام لمن؟ لخلايا الكلية + +89 +00:06:58,360 --> 00:07:05,000 +autotoxic سام للأذن يعني للعصب السمعي مثلا في + +90 +00:07:05,000 --> 00:07:09,920 +الأذن يكون متخصص لكن في النهاية هو تصنيف بحسب ال + +91 +00:07:09,920 --> 00:07:14,140 +site affected فابتفقنا + +92 +00:07:14,140 --> 00:07:16,780 +على ال enterotoxin و ال botulism و أنا بتقولكوا ال + +93 +00:07:16,780 --> 00:07:21,980 +botulism هو أقوى سم معروف للإنسان ينتج من قبل + +94 +00:07:21,980 --> 00:07:28,480 +كإنحيطو غالبا بتسمم الناس عبر تناول معلبات + +95 +00:07:28,480 --> 00:07:35,980 +المعلبات اللى بتكون مصنعة و لم نصل فاكرين لما + +96 +00:07:35,980 --> 00:07:39,780 +اتكلمت عن ال sterilization و قلنالكوا ان في مصطلح + +97 +00:07:39,780 --> 00:07:43,920 +اسمه commercial sterilization تعقيم تجارى لأن + +98 +00:07:43,920 --> 00:07:48,220 +التعقيم العادي ممكن عقب لكن ماتعطيش .. بظبط + +99 +00:07:48,220 --> 00:07:50,060 +فبالتالي + +100 +00:07:51,370 --> 00:07:57,950 +بصطرح تعقيم تجاري اشترت خلو الطعام من ال spores + +101 +00:07:57,950 --> 00:08:02,370 +الممرضة اللي من ضمنها مين؟ البوتلوني طيب produced + +102 +00:08:02,370 --> 00:08:07,130 +in vivo يعني داخل بسمنا احنا اتناولنا البكتيريا و + +103 +00:08:07,130 --> 00:08:10,010 +البكتيريا نمت جوا و بدأت تنجل ال toxin تبعها + +104 +00:08:10,010 --> 00:08:15,310 +داخلنا زي مين؟ زي ال deuterium من أحد أشهر وأخطر + +105 +00:08:15,310 --> 00:08:21,220 +الأمراض قبل 30 سنةوانزلوا كان الدفتيريا واحد من + +106 +00:08:21,220 --> 00:08:25,340 +الأمراض الخطيرة جدا أو أربعين سنة حتى اللي هو + +107 +00:08:25,340 --> 00:08:27,820 +الخناء بيسموه باللغة العربية تمام؟ أو العرب + +108 +00:08:27,820 --> 00:08:30,100 +بيسموها دفتيريا اللي هو الدفتيريا + +109 +00:08:33,130 --> 00:08:36,090 +اللي قلتلكوا من كتر ما ال .. ال .. ال .. + +110 +00:08:36,090 --> 00:08:40,970 +الانقباضات بتكون عنيفة قد ينكسر العمود الفقري + +111 +00:08:40,970 --> 00:08:44,710 +للمريض، تخيل العمود الفقري من .. عشان نكسره بدنا + +112 +00:08:44,710 --> 00:08:50,250 +قوة هائلة، لكن من شدة انقباض العضلات في المريض + +113 +00:08:50,250 --> 00:08:54,890 +اللي عندنا نفسه قد ينكسر العمود الفقريكانها الـ + +114 +00:08:54,890 --> 00:08:58,690 +streptococcal erythrogenic toxin، كلها بالثلاث + +115 +00:08:58,690 --> 00:09:01,730 +أنواع من التوكسين اللي ذكرناها دول الأمثلة، تنتج + +116 +00:09:01,730 --> 00:09:05,470 +in vitro، أفضل in vivo، والتأثير بتاعها بيكون + +117 +00:09:05,470 --> 00:09:09,290 +systemic، إيه يعني systemic؟ يعني بكل الجسم، بكل + +118 +00:09:09,290 --> 00:09:14,010 +الجسم، بيرم هناك بعض الميكروبات والتوكسين بيكون + +119 +00:09:14,010 --> 00:09:20,980 +لها local toxic effect، لأالتوكسين بنا systemic، + +120 +00:09:20,980 --> 00:09:23,880 +البكتيرى نفسها local، البكتيرى نفسها localized، بس + +121 +00:09:23,880 --> 00:09:28,560 +في الان localized ال .. البكتيريا والتوكسين زي + +122 +00:09:28,560 --> 00:09:31,880 +الكوليرا، الكوليرا في الأمعة مابتعزلهاش من الدم + +123 +00:09:31,880 --> 00:09:34,380 +ولا بتعزلها من أي مكان تاني، فقط من وين؟ من + +124 +00:09:34,380 --> 00:09:37,400 +الأمعة، فالتوكسين تبعها أيضا بكون تأثيره على + +125 +00:09:37,400 --> 00:09:42,390 +الأمعةنبنع اشريشيا كولاي هي non pathogenic في ال + +126 +00:09:42,390 --> 00:09:44,830 +intestinal tract لكن في منها exceptions أربع + +127 +00:09:44,830 --> 00:09:47,110 +أصنات، همد كلهم لاحقا، همد كلها بعد medical + +128 +00:09:47,110 --> 00:09:51,590 +microbiology واحدة منهم اسمها Enterotoxinic + +129 +00:09:51,590 --> 00:09:56,110 +Escherichia Coli، إذا الآن قدامنا على اللوحة تلت + +130 +00:09:56,110 --> 00:10:00,370 +نماذج، يا ريت نطلع عليهم كويس إذا ال toxin was + +131 +00:10:00,370 --> 00:10:04,070 +produced in vitro، إذا ال toxin was produced in + +132 +00:10:04,070 --> 00:10:09,910 +vivo، في مثلين، systemic و في أنها localالان انا + +133 +00:10:09,910 --> 00:10:14,530 +كنت في تصنيف ال toxins حد بيسموها A B Model ال A B + +134 +00:10:14,530 --> 00:10:18,690 +Model عبارة عن نموذج لتوكسين أو مجموعة توكسينات + +135 +00:10:18,690 --> 00:10:23,530 +خاصة مش خاصة هي ال Exotoxins مكوّنة من شقين بدا + +136 +00:10:23,530 --> 00:10:28,570 +الشق الأول بنسميه A واحفظه A Active والجزء التاني + +137 +00:10:28,570 --> 00:10:31,290 +B اللي هو زي ما موجود على الصدبرة أيضا + +138 +00:10:34,430 --> 00:10:37,650 +واطلع على ال animation اللى موجودة على يمين الشاشة + +139 +00:10:37,650 --> 00:10:42,250 +هلاجى إنه في خلية فيها receptor لمين ال receptor ل + +140 +00:10:42,250 --> 00:10:48,170 +B لكن عمل internalization لمن ل A و B هتكلم عنها + +141 +00:10:48,170 --> 00:10:54,670 +الآن هي B وهي A وهي ال receptor الارتباط لمن هيكون + +142 +00:10:54,670 --> 00:11:00,730 +الآن ل Bلكن ال internalization بتم لكل التوكسين + +143 +00:11:00,730 --> 00:11:05,330 +لكلهما بيصير release مين الأن اللي بيشتغل جوا؟ B + +144 +00:11:05,330 --> 00:11:10,330 +ولا A؟ B has no independent action مالوش أي عمل + +145 +00:11:10,330 --> 00:11:14,830 +مستقل فقط عملية إيصال التوكسين إلى المكان الموجود + +146 +00:11:14,830 --> 00:11:18,890 +طبعا العملية برضه كمان مهمة يعني لو شنا ال B من ال + +147 +00:11:18,890 --> 00:11:20,390 +A مش هيشتغل + +148 +00:11:23,360 --> 00:11:27,640 +مواضحة النقطة دي انه في عندي في ال toxin A B Model + +149 +00:11:27,640 --> 00:11:33,540 +A هو ال active و ال B بس بدنا نفهمش هانا ال + +150 +00:11:33,540 --> 00:11:40,540 +A نفترض انها في المنتصف و ال B ممكن تكون فجيها + +151 +00:11:40,540 --> 00:11:43,540 +تحتها على يمين على يسار ماحنا بحسب ما ننظر إليها + +152 +00:11:43,540 --> 00:11:48,960 +طيب لو كانت ال A في المنتصف و في عندي B و B و B و + +153 +00:11:48,960 --> 00:11:50,460 +B يعني أكتر من B + +154 +00:11:56,020 --> 00:12:01,260 +النسخ بيصير فرصة .. فرصة ان ال toxin يرتبط أسرع و + +155 +00:12:01,260 --> 00:12:05,120 +أسهل تمام؟ بالضبط .. بالضبط زي لو احنا بنجيب كورة + +156 +00:12:05,120 --> 00:12:09,380 +و ن .. و ن .. و نلحن فيها مسامير و نرميها على أي + +157 +00:12:09,380 --> 00:12:13,160 +جهة رميناها هلاجي مسامير وسط الأرض و مسامير + +158 +00:12:13,160 --> 00:12:16,600 +اقضالها في .. في اتجاهات متنوعة و مختلفة وبالتالي + +159 +00:12:16,600 --> 00:12:21,510 +فرصتها في الارتباط بتكون ايه؟ أعلىطيب برضه نسميه a + +160 +00:12:21,510 --> 00:12:25,890 +b model صحيح a one و five b's تمام لكن في الآخر هو + +161 +00:12:25,890 --> 00:12:31,030 +a و b طب لو كان a اتنين و خمسة b بيمشي الحال بيمشي + +162 +00:12:31,030 --> 00:12:35,810 +الحال بيبقى نفس المفهوم two a's تمام و يمكن a one + +163 +00:12:35,810 --> 00:12:39,770 +و a two اخدوا بالكوا a one و a two ايش معناهم ان a + +164 +00:12:39,770 --> 00:12:46,420 +بختلف عن a one بختلف عن a twoA1, A2, 5B's، الـ + +165 +00:12:46,420 --> 00:12:50,240 +5B's هم هما و ممكن زي ما حضرتك اتوقعتي انه ناجي A + +166 +00:12:50,240 --> 00:12:54,960 +واحدة و 5B's مختلفين و هذا بيصير .. ايش بيعمل هذا؟ + +167 +00:12:54,960 --> 00:13:00,560 +بيزيد من ال specificity تبعت للأنشجة المختلفة بصير + +168 +00:13:00,560 --> 00:13:04,500 +قدرة التوكسينة على الدخول الى أنشدة متنوعة اعلى + +169 +00:13:04,500 --> 00:13:07,620 +لما يكون في عنده اتنوة في ال B أو في ال binding + +170 +00:13:07,620 --> 00:13:13,920 +siteمن النماذج المشهورة جدا في علم ال microbiology + +171 +00:13:13,920 --> 00:13:17,140 +على ال mechanism of action ل A-B model اللي هو + +172 +00:13:17,140 --> 00:13:22,060 +deuterium toxin و طبعا في شبيه له اسمه pseudomonas + +173 +00:13:22,060 --> 00:13:26,420 +aeruginosa toxin A او X-toxin A كيف بيشتغلها + +174 +00:13:26,420 --> 00:13:30,160 +دولها؟ تقريبا بيشتغلوا بنفس القالية بس خدنا ناخد + +175 +00:13:30,160 --> 00:13:33,720 +ال deuterium كنموذج و التاني بنقول مثله بالضبط + +176 +00:13:33,720 --> 00:13:40,240 +تعرف ال protein synthesis؟ ال protein synthesisالـ + +177 +00:13:40,240 --> 00:13:43,400 +protein synthesis بيتلخص بإنه في عندي large + +178 +00:13:43,400 --> 00:13:47,720 +subunit و small subunit و messenger mRNA و بيصير + +179 +00:13:47,720 --> 00:13:52,500 +ارتباط بين التلتة component دولة و ببدأ ال .. ال + +180 +00:13:52,500 --> 00:13:55,700 +.. ال akyl aminotransfer يجي ينزل على ال ribosome + +181 +00:13:55,700 --> 00:13:58,640 +و يفرق حمولته من ال amino acid و ال amino acid + +182 +00:13:58,640 --> 00:14:03,180 +يرتبط ينتقل لقدر amino akyl و هكذا و يصير في إطالة + +183 +00:14:03,180 --> 00:14:09,380 +للسلسلةكلمة elongation factor بتعني عامل يساهم في + +184 +00:14:09,380 --> 00:14:15,980 +إطالة ال polypeptide اللي هو ال EF وهذا العامل من + +185 +00:14:15,980 --> 00:14:19,740 +ضرورات ال protein synthesis من ضرورات ال protein + +186 +00:14:19,740 --> 00:14:26,080 +synthesis في عملية اللي هو ال translation طيب + +187 +00:14:26,080 --> 00:14:29,240 +تخيلوا معا الوضع كان تاني ال diphtheria toxin + +188 +00:14:29,240 --> 00:14:34,880 +بتروح ترتبط بهذا ال factorو بتضيف إليه ADP-ripose + +189 +00:14:34,880 --> 00:14:40,480 +وعلى اللي بنسميها ADP-riposylation ADP اللي هو + +190 +00:14:40,480 --> 00:14:49,840 +adenosine diphosphate الآن ضفنا ADP-ripose لمين؟ + +191 +00:14:49,840 --> 00:14:55,640 +لل elongation factor 2 اللي كان active لما ضفناله + +192 +00:14:55,640 --> 00:15:00,120 +بطل active لما بطل active يبقى الآن بطل يكون في + +193 +00:15:00,120 --> 00:15:03,910 +دورة تصنيعة لبروتينطيب بالنسبة لي احنا بتتكلم + +194 +00:15:03,910 --> 00:15:07,610 +دايما هو باستمرار خلية بدون protein synthesis is a + +195 +00:15:07,610 --> 00:15:12,210 +doomed dead يعني محكم عليها بالموت لماذا محكم + +196 +00:15:12,210 --> 00:15:15,910 +عليها بالموت؟ كل ال enzymes اللي بتشتغل في الخلية + +197 +00:15:15,910 --> 00:15:19,990 +هي proteins وبالتالي بدون تصنيع enzymes أو other + +198 +00:15:19,990 --> 00:15:24,770 +component الخلية بالموت وبالتالي اي toxin + +199 +00:15:27,350 --> 00:15:33,450 +بيواجه تصنيع ال protein irreversibly بشكل غير قابل + +200 +00:15:33,450 --> 00:15:37,530 +للعكس، غير قابل للعكس، غالبا .. غالبا بيؤدي لموت + +201 +00:15:37,530 --> 00:15:38,710 +اللي هو ايه؟ الخلق + +202 +00:15:58,120 --> 00:16:00,920 +طب طبطبطبطبطبطبطبطبطبطبطبطبطبطبطبطبطبطبط + +203 +00:16:15,080 --> 00:16:19,360 +بترتبط بال adenylate cyclase enzyme، ارتباطها بال + +204 +00:16:19,360 --> 00:16:22,000 +adenylate cyclase enzyme مش بيعمل، بيعمله + +205 +00:16:22,000 --> 00:16:26,360 +activation لها، عكس الأولانية اللي كانت عمل بيه + +206 +00:16:26,360 --> 00:16:30,400 +activation، إذا بالارتباط، خدوا بالكمون التوكسين + +207 +00:16:30,400 --> 00:16:32,780 +في المركب، يا بعمله activation يا بعمله + +208 +00:16:32,780 --> 00:16:37,120 +inactivation، فهذا عملله activation، لما عملله + +209 +00:16:37,120 --> 00:16:42,480 +activation، راح حوّل ال ATP لcyclic AMPوالcycle + +210 +00:16:42,480 --> 00:16:46,980 +kmb انشرحته المرة الماضية قلنا بيزيد من اللي هيطلع + +211 +00:16:46,980 --> 00:16:52,080 +على الصبورة على اللوحة أيضا في ان هنا كمية الانتاج + +212 +00:16:52,080 --> 00:16:56,480 +لل Sodium و لل chloride و لل potassium و لل + +213 +00:16:56,480 --> 00:17:01,220 +carbonate زادت في ال human of the intestine فالجسم + +214 +00:17:01,220 --> 00:17:05,800 +مضطر ينزل معاشر كمان كمية كبيرة من من الماء وبتالي + +215 +00:17:05,800 --> 00:17:09,550 +دياريا واز producedإذا هذه الـ mechanism by which + +216 +00:17:09,550 --> 00:17:16,030 +ال Vibrio cholera produces دياري اكيوميليش .. نجيب + +217 +00:17:16,030 --> 00:17:18,550 +معكوس، نقول دياريا was produced، ليش فيه ديارية؟ + +218 +00:17:18,550 --> 00:17:21,550 +لأنه فيه ميه، ليش فيه ميه كبيرة، كمية كبيرة؟ لأنه + +219 +00:17:21,550 --> 00:17:24,810 +فيه electrolytes طب ليش ال electrolytes اتراكمت في + +220 +00:17:24,810 --> 00:17:29,130 +المكان هذا؟ لأنه فيه كمية Cyclic AMP أُنتجات بشكل + +221 +00:17:29,130 --> 00:17:32,730 +كبير، لماذا؟ لأنه فيه cell activation لليليت + +222 +00:17:32,730 --> 00:17:35,530 +الcyclase enzyme، ميه اللي عمل activation؟ اللي هو + +223 +00:17:35,530 --> 00:17:41,150 +ال cholera toxicوهناك Escherichia coli بتنتج + +224 +00:17:41,150 --> 00:17:45,250 +توكسين اسمه cholera-like toxin اللي هي البكتيريا + +225 +00:17:45,250 --> 00:17:49,090 +اللي اسمها enterotoxygenic escherichia coli + +226 +00:17:49,090 --> 00:17:54,090 +enterotoxygenic escherichia coli و بين قوسين حطوا + +227 +00:17:54,090 --> 00:17:57,730 +كلمة labile ماعرفش مولود على اللابحة كلمة labile + +228 +00:17:57,730 --> 00:18:04,970 +شايفينها? E coli labile toxinكلمة labile يعني قابل + +229 +00:18:04,970 --> 00:18:10,230 +للتدمير .. قابل للتدمير لما عزلوا ال E.coli toxins + +230 +00:18:10,230 --> 00:18:14,370 +لجوا منها نوعين لجوا نوع stable التكسين تبعها لما + +231 +00:18:14,370 --> 00:18:19,110 +نحطه بالحرارة اللي مدملش فسموه S أو stable toxin + +232 +00:18:19,110 --> 00:18:24,070 +والتاني بتأثر بالحرارة فسموه labile toxin إذا ال + +233 +00:18:24,070 --> 00:18:25,930 +labile toxin هو اللي بتتكلم عنه + +234 +00:18:32,130 --> 00:18:36,110 +يعني هيمروا معاها في المستقبل oxygen labile هداك + +235 +00:18:36,110 --> 00:18:39,610 +هنقوله عنها heat labile تتأثر بالحرارة أو تتأثر + +236 +00:18:39,610 --> 00:18:48,590 +بمين؟ بال oxygen طيب نجي لتكسين تنتجه الشجلة أو + +237 +00:18:48,590 --> 00:18:50,950 +برضه نوع آخر من الشريشا كولا يسمونه + +238 +00:18:50,950 --> 00:18:56,270 +enterohemorrhagic-schericia-kola إذن نتكلم عن two + +239 +00:18:56,270 --> 00:19:03,990 +toxins بينتلكوا نفس ال mechanism ofبرضه في المحصلة + +240 +00:19:03,990 --> 00:19:08,190 +بيوقفوا ال protein synthesis هنا لكن بطريقة مختلفة + +241 +00:19:08,190 --> 00:19:11,990 +عن الدفتيريا تتذكروا الدفتيريا اشتغلت على مين؟ على + +242 +00:19:11,990 --> 00:19:16,710 +elongation factor 2 هذه اشتغلت على مين؟ على انها + +243 +00:19:16,710 --> 00:19:20,570 +عملت تكسير للريبوزومة ال RNA اللي في الريبوزوم + +244 +00:19:20,570 --> 00:19:22,990 +المهمين اللي بيصنع ال protein synthesis أساسا + +245 +00:19:22,990 --> 00:19:26,630 +الريبوزوم إذا خربنا الريبوزوم و بقوا فينا ال + +246 +00:19:26,630 --> 00:19:29,690 +protein synthesis يبقى وجفنا و أصنع فرده ال + +247 +00:19:29,690 --> 00:19:37,430 +protein لكن بطريقةمختلفة عن اللي هو ال .. نعم نجد + +248 +00:19:37,430 --> 00:19:42,190 +البوتلونيو توكسين البوتلونيو توكسين محتاج مننا + +249 +00:19:42,190 --> 00:19:46,600 +يمكن ان شاء الله نقدر نوركوا ال animationبنطلع على + +250 +00:19:46,600 --> 00:19:49,940 +ال animation اللي قدامنا هذه ونشوف ال X-Toxins + +251 +00:19:49,940 --> 00:19:53,700 +طبعا قلنا they are produced while the cells are + +252 +00:19:53,700 --> 00:19:58,180 +alive and their effect may be localized or may be + +253 +00:19:58,180 --> 00:20:01,780 +systemic على حسب اللي هو النوع التوكسين زي ال + +254 +00:20:01,780 --> 00:20:03,980 +Vibrio كده اللي قلنا localized بينما التتانوس كان + +255 +00:20:03,980 --> 00:20:09,580 +إيه؟ كان سيستميك تصنيف ال X-Toxin قلنا ممكن يكون + +256 +00:20:09,580 --> 00:20:15,340 +حسب اللي هو ال site affectedيعني مثلا لما قلنا انه + +257 +00:20:15,340 --> 00:20:21,060 +بصيب ال nervous system سمنة neurotoxin إذا بصيب ال + +258 +00:20:21,060 --> 00:20:24,700 +intestine سمنة ال intestine ال intestine ال + +259 +00:20:24,700 --> 00:20:25,140 +intestine ال intestine ال intestine ال intestine + +260 +00:20:25,140 --> 00:20:25,380 +intestine ال intestine ال intestine ال intestine + +261 +00:20:25,380 --> 00:20:25,540 +ال intestine ال intestine ال intestine ال + +262 +00:20:25,540 --> 00:20:26,000 +intestine ال intestine ال intestine ال intestine + +263 +00:20:26,000 --> 00:20:26,980 +intestine ال intestine ال intestine ال intestine + +264 +00:20:26,980 --> 00:20:27,020 +ال intestine ال intestine ال intestine ال + +265 +00:20:27,020 --> 00:20:27,840 +intestine ال intestine ال intestine ال intestine + +266 +00:20:27,840 --> 00:20:27,860 +intestine ال intestine ال intestine ال intestine + +267 +00:20:27,860 --> 00:20:27,900 +intestine ال intestine ال intestine ال intestine + +268 +00:20:27,900 --> 00:20:27,940 +intestine ال intestine ال intestine ال intestine + +269 +00:20:27,940 --> 00:20:28,040 +ال intestine ال intestine ال intestine ال + +270 +00:20:28,040 --> 00:20:28,320 +ال intestine ال intestine ال intestine ال + +271 +00:20:28,320 --> 00:20:32,660 +ال intestine ال intestine ال intestine ال + +272 +00:20:32,660 --> 00:20:36,340 +intestine ال + +273 +00:20:44,400 --> 00:20:47,540 +التصنيف الان by structure and function هذا تصنيفه + +274 +00:20:47,540 --> 00:20:51,720 +كويس كثير احنا قلنا اب موديل صح الاب موديل اذا كان + +275 +00:20:51,720 --> 00:20:55,620 +تركيبه من قطعتين اساسيتين active و binding بيسمي + +276 +00:20:55,620 --> 00:20:59,280 +اب توكسين وفي membrane disruption اللي هو بيأثر + +277 +00:20:59,280 --> 00:21:02,820 +على ال .. ال .. ال .. ال membrane خدنا ناخد الأول + +278 +00:21:02,820 --> 00:21:06,540 +حاجة اللي هو الاب توكسين وطبعا الأخر اللي هو كان + +279 +00:21:06,540 --> 00:21:10,720 +super antigen هاي الالومان والجزء التاني هو ال بي + +280 +00:21:10,720 --> 00:21:13,260 +لومان ال بي كان لل binding + +281 +00:21:16,520 --> 00:21:20,240 +احنا معناه نفهم ال concepts بشكل أساسي عشان نقدر + +282 +00:21:20,240 --> 00:21:24,080 +نفهم بعض النقاط المتلتبة على هذا ال toxin لاحقا + +283 +00:21:24,080 --> 00:21:27,860 +بال endosomes بعدين بيصيرنه release و اللي هو ال a + +284 +00:21:27,860 --> 00:21:29,940 +هو اللي بيقوم بالمهمة تبعه + +285 +00:21:37,250 --> 00:21:41,330 +طبعا ال effects مختلفة ال effects مختلفة بحسب + +286 +00:21:41,330 --> 00:21:44,530 +التوكسين لكن احنا بنوصل الفكرة ان ال B هو اللي دخل + +287 +00:21:44,530 --> 00:21:48,190 +A و بعدين صارله release ال membrane disruption + +288 +00:21:48,190 --> 00:21:51,910 +فكان ال phospholipase و ال lycethinase و ال + +289 +00:21:51,910 --> 00:21:55,210 +leukocydenes كل مدلة بيشتغلوا على مين؟ على ال + +290 +00:21:55,210 --> 00:21:59,030 +membranes فلو بيعملوا complex معين بيفتح ال + +291 +00:21:59,030 --> 00:22:02,710 +membrane بصير release لمين؟ لل component سبعة + +292 +00:22:02,710 --> 00:22:04,930 +الخلية هذا بصير موط ليه؟ الخلية + +293 +00:22:08,300 --> 00:22:11,480 +النوع التالت هو ال super antigen وانا بدأ اشرح هو + +294 +00:22:11,480 --> 00:22:13,880 +يعني مش كتير واضح اللي .. اللي .. اللي موجود في ال + +295 +00:22:13,880 --> 00:22:18,120 +animation بس برضه كويس اننا نطلع عليه نجح فهنا + +296 +00:22:18,120 --> 00:22:24,640 +الان لما انا .. لو انا في المحاضرة بدوزع حدث معين + +297 +00:22:24,640 --> 00:22:31,060 +على الطلاب فبقول لزملتكوا انت عليكي واجب X وانت + +298 +00:22:31,060 --> 00:22:37,700 +واجب Y و Z و A و C بنصق الموضوعلكن لو افترضنا .. + +299 +00:22:37,700 --> 00:22:42,480 +لو افترضنا ان الشخص اللي بوزع المهم مش موجود و + +300 +00:22:42,480 --> 00:22:46,660 +ايجا واحد انت عليك مهمة X وانت مهمة X وانت مهمة X + +301 +00:22:46,660 --> 00:22:50,740 +وانت مهمة X بصير اللي بيعملوا نفس المهمة كثق + +302 +00:22:50,740 --> 00:22:59,990 +فالمنتج تبع المهمة برضه كتيراحنا بنعرف .. بنعرف ان + +303 +00:22:59,990 --> 00:23:04,130 +ال .. ال .. ال immune system is orchestrated .. + +304 +00:23:04,130 --> 00:23:06,070 +orchestrated يعني منظم زي orchestra بتاخد + +305 +00:23:06,070 --> 00:23:08,710 +orchestra اللي بيبقى مسك العصايا و ي .. و ينظم ال + +306 +00:23:08,710 --> 00:23:13,290 +.. ال .. اللي باعزفه موسيقى ال immune system + +307 +00:23:13,290 --> 00:23:16,410 +بيوصفوه بهذه الطريقة انه orchestrated انه في خلايا + +308 +00:23:16,410 --> 00:23:24,430 +بتنظم عمل الخلايا الأخرى إنتاج T cell لوظيفةإنها + +309 +00:23:24,430 --> 00:23:29,510 +تجيب تتفرز cytokines ال cytokines اتنادي فيها على + +310 +00:23:29,510 --> 00:23:33,890 +other cells cytokines specific تخيلوا كل الصف + +311 +00:23:33,890 --> 00:23:39,430 +سيطلع cytokines زي ما كل الصف بصير صوت حد هيفهم .. + +312 +00:23:39,430 --> 00:23:44,670 +هيفهم انه جهاز المناعة كله هيجي على المكان طيب + +313 +00:23:44,670 --> 00:23:49,870 +احنا ردة الفعل اللي المطلوبة هي ردة فعل تتنسب مع + +314 +00:23:49,870 --> 00:23:55,300 +حجم ال antigenلما الانتجين هذا يعمل رد فعل مناعية + +315 +00:23:55,300 --> 00:23:59,600 +ضخمة بيلاقيش بسرعانها بسرعانها exaggerated immune + +316 +00:23:59,600 --> 00:24:04,860 +response يعني رد فعل مناعية مبالغ بها تؤدي إلى + +317 +00:24:04,860 --> 00:24:08,540 +ماذا؟ إلى release لاهستامين و inflammatory + +318 +00:24:08,540 --> 00:24:17,080 +substances و قد تؤدي إلى shock and death تخيلوا؟ + +319 +00:24:17,080 --> 00:24:21,720 +من ايه؟ من انه ليه ليه يسموه super antigen؟أكيد + +320 +00:24:21,720 --> 00:24:25,880 +يقولوا super يعني حاجة مميزة، means تو الأساسية + +321 +00:24:25,880 --> 00:24:31,240 +فقط أنه بيقدر يعمل non-specific activation of news + +322 +00:24:52,510 --> 00:24:56,870 +أما لما يكون localized الأمر متابعه بيكون ان شاء + +323 +00:24:56,870 --> 00:24:57,330 +الله بسيط + +324 +00:25:00,690 --> 00:25:06,470 +الخلايا يشتر فيها انقسامات كمية كبيرة produced من + +325 +00:25:06,470 --> 00:25:10,450 +ال cytokines لا تتنسب إطلاقا مع حجم ال threat + +326 +00:25:16,390 --> 00:25:20,310 +لحظوا انا لففكت على مين؟ على كل ال pc لأن كل جهاز + +327 +00:25:20,310 --> 00:25:23,670 +المناعة بيتأثر بهذا و طبعا بيصير فيه ارتفاع في + +328 +00:25:23,670 --> 00:25:27,190 +درجة حرارة اللي هو ال pc مدام صارنا داخلين في ال + +329 +00:25:27,190 --> 00:25:31,530 +animation نكمل و نشوف ال endotoxin و ذكركم احنا + +330 +00:25:31,530 --> 00:25:37,840 +نستعرض اللوحة، يا عزيزي نتركز على اللوحةإن هو + +331 +00:25:37,840 --> 00:25:40,480 +عبارة عن component من ال cell من ال outer cell من + +332 +00:25:40,480 --> 00:25:43,080 +بنيه ب gram negative بكتيريا وكان مكوّن من O-sided + +333 +00:25:43,080 --> 00:25:45,840 +chain و ال lipid A، ال lipid A هو ال endotoxin + +334 +00:25:45,840 --> 00:25:50,980 +الماكروفاج بتاكل البكتيريا أو ال phagocyte، جوا + +335 +00:25:50,980 --> 00:25:55,260 +الماكروفاج بيصير تم عملية اللي هو التحليل لل .. ال + +336 +00:25:55,260 --> 00:25:59,990 +.. ال ..البكتيريا وتكسيرها بيصير release لمن؟ لل + +337 +00:25:59,990 --> 00:26:04,910 +endotoxin بيطلع انرسحانس الو Interleukin ال + +338 +00:26:04,910 --> 00:26:08,310 +Interleukin بيروح على ال hypothالمس في الدماغ + +339 +00:26:08,310 --> 00:26:11,710 +والان ال hypothالمس مسئول عن ال thermal regulation + +340 +00:26:11,710 --> 00:26:17,670 +في الجسم فبترتفع درجة الحرارة أول حاجة بترتفع درجة + +341 +00:26:17,670 --> 00:26:22,570 +الحرارة طبعا في أشياء أخرى كتيرة من ضمنها العميدي + +342 +00:26:22,570 --> 00:26:24,770 +الفاجو سايتوز الكتيرة ال antibodies اللي بيصير + +343 +00:26:24,770 --> 00:26:30,520 +تتنشفلكن في نقطة كتير مهمة، وجود البكتيريا + +344 +00:26:30,520 --> 00:26:36,750 +والتوكسين بيقدي الى انخفاض في طاقة الدمأحد أهم + +345 +00:26:36,750 --> 00:26:40,950 +أسباب الخطورة في ال endotoxin هو انخفاض اللي هو ال + +346 +00:26:40,950 --> 00:26:42,450 +blood pressure اللي هو الأصل اللي .. الأصل اللي + +347 +00:26:42,450 --> 00:26:46,630 +لازم يكون على كده؟ 120 على 80 مثلا أو 110 على 70 + +348 +00:26:46,630 --> 00:26:50,730 +بحسب الجنس ذاك على المنثى، لاحظوا أنا مش بصير أنا + +349 +00:26:50,730 --> 00:26:55,370 +جاعد، بسبب هبوط في ضغط الدم، طب لما يصل أنا ضغط + +350 +00:26:55,370 --> 00:27:00,830 +الدم لهذا .. لهذه الدرجة، أي حركة قد تفقدنا + +351 +00:27:00,830 --> 00:27:04,710 +التوازن أو ممكن حتى نصل بينا إلى shock and death + +352 +00:27:05,210 --> 00:27:08,850 +فال endotoxin يا بنات التوكسين تبع ال gram + +353 +00:27:08,850 --> 00:27:12,410 +negative بكتيريا اللي احنا اتعلمناه في أول الفصل و + +354 +00:27:12,410 --> 00:27:16,370 +قلنا عنه جزء من ال component تبع كل gram negative + +355 +00:27:16,370 --> 00:27:21,990 +بكتيريا لو البكتيريا موجودة فيها جسمنا و علجناها و + +356 +00:27:21,990 --> 00:27:28,450 +قضينا عليها هذا وحده لا يكفيلأنه قد نقتل المريض + +357 +00:27:28,450 --> 00:27:32,210 +بقتل الألمين للبكتيريا وبالتالي بدنا نكون عندنا + +358 +00:27:32,210 --> 00:27:36,390 +اطلاع او معرفة بأن ال endotoxin له effects كثيرة + +359 +00:27:36,390 --> 00:27:40,090 +من ضمنها كذا مرة low blood pressure high + +360 +00:27:40,090 --> 00:27:44,010 +temperature وفي عندنا اللي هو coma and death طب + +361 +00:27:44,010 --> 00:27:48,470 +خليني بس نوضح النقطة هذه الان انتوا بتعرفوا مركبة + +362 +00:27:48,470 --> 00:27:52,310 +ال acetylcholine اسمها فيه؟ ايش هو دي بتقولها ده؟ + +363 +00:27:52,310 --> 00:27:58,520 +neurotransmitter ايش هي transmission؟Bath، bath + +364 +00:27:58,520 --> 00:28:02,560 +يعني نقل إشارة، فال neurotransmitter هو نقل عصبي، + +365 +00:28:02,560 --> 00:28:06,500 +نقل عصب أو إشارة عصبية، ال botulinum toxin حضرتوش + +366 +00:28:06,500 --> 00:28:10,760 +بيعمل .. بيعمل inhibition للأزيطائي الكلي، يبقى + +367 +00:28:10,760 --> 00:28:14,400 +مافيش نقل للإشارة العصبية، مافيش إرسال، مافيش + +368 +00:28:14,400 --> 00:28:17,540 +إرسال .. مافيش إرسال يبقى مافيش استجبال، وده لا .. + +369 +00:28:17,540 --> 00:28:21,080 +وده مافيش استجبال يبقى فينا شلل، أي نوع من الشلل + +370 +00:28:22,710 --> 00:28:25,530 +الacetylcholine المفروض أن يعطينا إشارة + +371 +00:28:25,530 --> 00:28:28,450 +للcontraction، ما تنفيش contraction، إيش معناه + +372 +00:28:28,450 --> 00:28:32,650 +حاجة تكون فينا؟ relaxation، شلل بيسموه أخر جملة + +373 +00:28:32,650 --> 00:28:36,010 +flaccid paralysis، فالـ flaccid paralysis معناته + +374 +00:28:36,010 --> 00:28:40,200 +شلل إرتخائي، شلل إرتخائيو انا فاكرين لما اتكلمنا + +375 +00:28:40,200 --> 00:28:45,020 +عن ال toxin هذا قلنالكم بيعطوه ك .. للتجميل في ال + +376 +00:28:45,020 --> 00:28:49,760 +botox او ما يسمى ال botox و قلنا سبب استخدامه هو + +377 +00:28:49,760 --> 00:28:53,100 +ان التجايد هي عبارة عن .. يعني بتكون تقلصات + +378 +00:28:53,100 --> 00:28:56,300 +contracted skin او .. او muscles في ال skin فهو + +379 +00:28:56,300 --> 00:28:59,080 +لما لها عملته اللي هو relaxation لكن هذا قلنا كلمة + +380 +00:28:59,080 --> 00:29:04,100 +مرة قوله diluted بيكون في فترة مؤقتةلكن بنفهم إن + +381 +00:29:04,100 --> 00:29:06,960 +الـ mechanism of action كانت مبنية على مين؟ على + +382 +00:29:06,960 --> 00:29:12,680 +إنه فقدان الإشارة العصبية عن طريق منع إنتاج من الـ + +383 +00:29:12,680 --> 00:29:17,060 +sparkling أنا بقولكوا احفظوا لإنه اللي وراه على + +384 +00:29:17,060 --> 00:29:25,700 +طول اللي هو التتانوس عكسه تمانع inhibits + +385 +00:29:25,700 --> 00:29:30,020 +glycine release إيش مشكلة ال glycine هذا؟ الآن + +386 +00:29:30,020 --> 00:29:34,960 +مافيش botulism فيه normalفي normal contraction و + +387 +00:29:34,960 --> 00:29:38,200 +relaxation ال contraction بينتج على أنه في + +388 +00:29:38,200 --> 00:29:43,340 +أسيتالكولين تمام؟ و ال relaxation لجليسين بيقول ل + +389 +00:29:43,340 --> 00:29:46,720 +.. بيقول لجليسين بيقول لأسيتالكولين بكف خلاص روح + +390 +00:29:46,720 --> 00:29:50,120 +تمام؟ فبصير العملية فيها نوع من إيه؟ من الإتزان + +391 +00:29:50,120 --> 00:29:54,400 +الأسيتالكولين بيعطي الإشارة و أنا بقطع النوم + +392 +00:29:54,400 --> 00:29:59,120 +الإشارة فبصير العملية طبيعية طيب في ال tetanus + +393 +00:29:59,120 --> 00:30:05,100 +الوضع اختلف في أسيتالكولينوعمل contraction احنا + +394 +00:30:05,100 --> 00:30:09,560 +مانعنا مين؟ مانعنا اللي بيمنع ال acetylcholine + +395 +00:30:09,560 --> 00:30:13,800 +فصار عايش عندي contraction على طول بيبقى الشرر + +396 +00:30:13,800 --> 00:30:16,400 +اللي صار عندي شرر مختلف تماما عن الأولاني هدا + +397 +00:30:16,400 --> 00:30:19,860 +الأولاني إيش كان؟ كان relaxed أو سبب لها flaccid + +398 +00:30:19,860 --> 00:30:24,460 +paralysis هذا بيسموه spastic، spastic يعني انقباضي + +399 +00:30:24,460 --> 00:30:29,580 +وعشان قلتلكوا أنه ال spasm أو ال .. ال .. ال .. + +400 +00:30:30,210 --> 00:30:34,730 +التقلصات أو التشهنجات اللى تنتج عند مرضى التتانوس + +401 +00:30:34,730 --> 00:30:40,110 +قد تكون شهيدة وتكسر العمود اللى هو Alpha ال + +402 +00:30:40,110 --> 00:30:42,850 +animation اللى قدامنا هذا على فكرة واضحة برضه + +403 +00:30:42,850 --> 00:30:48,850 +بتعطينا فكرة على اللى هو العملية بتاعة الدهام + +404 +00:30:48,850 --> 00:30:54,310 +الأولفي عندي هناش ال acetylcholine receptor و هذا + +405 +00:30:54,310 --> 00:30:58,810 +ال glycine اللي هو ال glycine لإن ال toxin بيرفبط + +406 +00:30:58,810 --> 00:31:02,210 +بي هذا المنطقة و بيمنع مين؟ بيمنع ال glycine + +407 +00:31:02,210 --> 00:31:06,430 +فبالتالي بيصير فيه إنتاج مستمر لل acetylcholine و + +408 +00:31:06,430 --> 00:31:09,490 +action مستمر لل acetylcholine و بالتالي مافيش أنها + +409 +00:31:09,490 --> 00:31:14,830 +ان كان هي لأ فإنها contraction مستمر و لا يوجد + +410 +00:31:14,830 --> 00:31:20,090 +relaxationوهذا ال .. ال .. مريض .. مريض تتنوس بي + +411 +00:31:20,090 --> 00:31:25,670 +.. يعني بيمر براحة كتيرة من ضمنها اللي هو ال + +412 +00:31:25,670 --> 00:31:29,330 +paralysis لعضلات الفك لذلك باللي جيه بالعربي + +413 +00:31:29,330 --> 00:31:33,670 +بيسموه الكزاز ليش؟ لإنه كز ع أصنانه لإن عضلات .. + +414 +00:31:33,670 --> 00:31:37,890 +أو بيسموه بالإنجليزي fixed smile الإبتسامة الثابت + +415 +00:31:37,890 --> 00:31:41,330 +دايما المريض بيكون مبتسم وعشانك مش كل واحد بيبتسم + +416 +00:31:41,330 --> 00:31:44,450 +إنه ممكن .. يعني يكون مبتسم إنه سعيد ربما يكون + +417 +00:31:44,450 --> 00:31:52,620 +عنده تتنوسطيب ال X-toxin في ال .. ال .. في ال + +418 +00:31:52,620 --> 00:31:56,000 +extra cellular matrix of connective tissue مثال + +419 +00:31:56,000 --> 00:32:01,540 +احنا درسنا في ال invasions تمام بعض التوكسينز + +420 +00:32:01,540 --> 00:32:05,140 +الكرستوديوم بقى رفرنجز على سبيل المثال بتنتج + +421 +00:32:05,140 --> 00:32:07,240 +collagenase مثلا ال collagenase درسناها في وين؟ + +422 +00:32:07,240 --> 00:32:10,780 +كان .. اه في ال invasionفي ال invasion حاكيناه + +423 +00:32:10,780 --> 00:32:15,060 +الآن و حاكيناه و اسمه واضح ان هو عبارة عن ايه؟ عن + +424 +00:32:15,060 --> 00:32:20,700 +انزين بولوجينايز بيكسر لكن كونه كونه بيقتل الخلايا + +425 +00:32:20,700 --> 00:32:23,580 +نتيجة نتيجة ان هي بتقتل الخلايا فممكن نعتبره برضه + +426 +00:32:23,580 --> 00:32:28,100 +ايه؟ ممكن نعتبره toxins يعني enzymes with toxic + +427 +00:32:28,100 --> 00:32:32,640 +activity الاستبتاكوكاس حياليرونيديز و بيكسر + +428 +00:32:32,640 --> 00:32:35,220 +الهياليرونيك اسد اللي برضه بلبط الخلايا ببعضها + +429 +00:32:35,220 --> 00:32:42,670 +البعضmembrane-damaging-toxins شوفناهم في ال + +430 +00:32:42,670 --> 00:32:45,650 +animation مش بيقولنا احنا ايه بيو موديل و membrane + +431 +00:32:45,650 --> 00:32:49,710 +و بعدين بيقولنا super-antigens زي ال proteases + +432 +00:32:49,710 --> 00:32:54,970 +كلمة ال proteases هي عنوان لما احنا بيقوله برهات + +433 +00:32:54,970 --> 00:33:00,630 +فلوس املة بيتكلم عن دنار املة و دولار املة و شكل + +434 +00:33:00,630 --> 00:33:05,800 +املة و درهم املةفلكن في الآخر فيه لهم عنوان رئيسي + +435 +00:33:05,800 --> 00:33:09,000 +لما بيقول ال proteases هي كل الإنزيمات القادرة على + +436 +00:33:09,000 --> 00:33:12,180 +تكسير ال polypeptide أو ال proteinات فاهمين؟ في + +437 +00:33:12,180 --> 00:33:15,580 +منها أصناف متخصصة بتكون في منها أصناف متخصصة + +438 +00:33:15,580 --> 00:33:17,960 +phospholipases وانا حاطط بين قوسين اللي هو ال + +439 +00:33:17,960 --> 00:33:21,640 +acetinase اللي قولنا عنه واحد من ال phospholipases + +440 +00:33:21,640 --> 00:33:25,540 +وفي منهم detergent like action ايش يعني ال + +441 +00:33:25,540 --> 00:33:29,700 +detergent like action؟ احكي + +442 +00:33:37,270 --> 00:33:39,130 +وهو دي أماق يقولي احنا هنقولي اشتغلوا كال .. + +443 +00:33:39,130 --> 00:33:44,690 +كالمناطق تانية، نعم، بس كيف المناطق بيشتغل؟ الشكل + +444 +00:33:44,690 --> 00:33:49,610 +هو بدنا quiz، بدنا quiz أيش، ديك مفاجئ، عشان يحفظ + +445 +00:33:49,610 --> 00:33:51,630 +ال .. ال .. الذاكرة، عموما + +446 +00:33:55,680 --> 00:33:59,420 +كان فيه إله Hydrophilic و Hydrophobic و ال cell + +447 +00:33:59,420 --> 00:34:01,720 +membrane تبعنا ال phospholipids تبعتنا كانت + +448 +00:34:01,720 --> 00:34:04,300 +Hydrophilic و Hydrophobic ال interaction between + +449 +00:34:04,300 --> 00:34:07,280 +the two بيعمل destruction لمين؟ لإلمنا نفسه ما هو + +450 +00:34:07,280 --> 00:34:13,880 +ال detergent like action طيب ناخد واحد من الأمثلة + +451 +00:34:13,880 --> 00:34:16,060 +اللي هو ال eclostridium paraphringens + +452 +00:34:16,060 --> 00:34:19,500 +phospholipids بالمناسبة ال eclostridium + +453 +00:34:19,500 --> 00:34:23,830 +paraphringens مررت معانا قبل هيك تحت أي عنوانأو + +454 +00:34:23,830 --> 00:34:32,030 +تحت أي فكرة أو تحت أي مرض مين بتذكر؟ + +455 +00:34:32,030 --> 00:34:34,350 +خدوا بأكوا مبررات ال quiz و قولوا مانا قاعد بالزيد + +456 +00:34:34,350 --> 00:34:37,370 +الفوسفول + +457 +00:34:37,370 --> 00:34:40,230 +.. هقولوا .. هقولوا مول مرة معانا .. لما اتكلمنا + +458 +00:34:40,230 --> 00:34:43,290 +عن ال endosport اتكلمنا عن ال endosport اتكلمنا + +459 +00:34:43,290 --> 00:34:48,410 +على twoGenera, Bacillus و Clostridium و مضمن ال + +460 +00:34:48,410 --> 00:34:52,610 +Clostridium تحدث عن ثلاث أصناف Perifringens و + +461 +00:34:52,610 --> 00:34:56,450 +Botulonium و Tetanus وقلنالكوا ال Perifringens هي + +462 +00:34:56,450 --> 00:35:02,190 +المسؤولة عن الجاز Gangrene الغرينة الغازية و أشهر + +463 +00:35:02,190 --> 00:35:08,170 +توكسين عندها هو ال phospholipase C اللي من خلالها + +464 +00:35:08,170 --> 00:35:14,080 +بتفكك الخلايا عن بعضها أو تقتل الخلاياطبعا خدوا + +465 +00:35:14,080 --> 00:35:18,480 +بالك يا بنات قتل الخلايا بيخدم غلطين انه .. انه .. + +466 +00:35:18,480 --> 00:35:21,840 +انه .. ال .. ال .. ال .. الخلايا اللي ماتت هتشكل + +467 +00:35:21,840 --> 00:35:27,280 +ايش لان؟ مادة غذائية مناسبة للبكتيريا، انت عارفين + +468 +00:35:27,280 --> 00:35:31,020 +على ايش بنربي احنا و بنمي الكلسترديوم؟ بنميها على + +469 +00:35:31,020 --> 00:35:35,940 +قطع اللحم، بنجبطيلها لحم تمام شجرة صغيرة و + +470 +00:35:35,940 --> 00:35:40,790 +بنغليها، اسم ال media is chopped meat brothبتحب + +471 +00:35:40,790 --> 00:35:43,710 +البروتينات بشكل كبير و اللحم مليان بروتينات تمام؟ + +472 +00:35:43,710 --> 00:35:46,790 +اللحوم فلما اتنوت خلايا العضلات بتبعتها اللي هي + +473 +00:35:46,790 --> 00:35:50,310 +اللحم تمام هي في واقع الأول مابتصبت غرضين، الغرض + +474 +00:35:50,310 --> 00:35:55,310 +الأول انه بتعمل invasion، رقم اتنين بتحصل عليها + +475 +00:35:55,310 --> 00:35:59,570 +موال مجدية من الخلايا اللي ماتت، ال phospholipase + +476 +00:35:59,570 --> 00:36:02,750 +له multiple functions، it destroys blood + +477 +00:36:06,040 --> 00:36:09,380 +Stops influx of inflammatory cells عنده إمكانية + +478 +00:36:09,380 --> 00:36:14,960 +يقول الخلايا اللي جاية تعمل ردد فعل منعية، يقولها + +479 +00:36:14,960 --> 00:36:20,200 +أنك واقفي، ماتمش هام It helps، أنا غلطان في الجملة + +480 +00:36:20,200 --> 00:36:23,140 +هنا، it helps in creating an aerobic environment + +481 +00:36:23,140 --> 00:36:28,940 +بيساعد في خلق بيئة لا هوائي، لأن البكتيريا أجمل + +482 +00:36:28,940 --> 00:36:33,040 +هذه البكتيريا لا هوائي، وبالتالي هي محتاجة إلى هذه + +483 +00:36:33,040 --> 00:36:36,220 +البيئةallows growth of this anaerobic طبعا + +484 +00:36:36,220 --> 00:36:38,860 +creating anaerobic environment بيساعد على ان + +485 +00:36:38,860 --> 00:36:45,180 +البكتيريا تنمو في هذه البيئة اللهوة طيب + +486 +00:36:45,180 --> 00:36:50,420 +مازلنا في ال exotoxin ومتكلم على .. ركزوا معايا لو + +487 +00:36:50,420 --> 00:36:55,700 +واحد أكل طعام بيحتوي على ال botulinum toxin ايش + +488 +00:36:55,700 --> 00:37:01,870 +حله؟ ايش علاجه؟ان نعطيه antitoxin زى واحد جرس طوحى + +489 +00:37:01,870 --> 00:37:08,030 +او عقرب نعطيه antitoxin ال antitoxin شو منه ده؟ + +490 +00:37:08,030 --> 00:37:12,330 +عبارة عن antibodies بسموها + +491 +00:37:12,330 --> 00:37:17,830 +neutralizing antibody ليش سموها neutralizing؟ + +492 +00:37:20,660 --> 00:37:22,920 +فالان ال antibodies اما بيقولون عنها antibodies + +493 +00:37:22,920 --> 00:37:26,920 +عادية او بيقولون عنها antitoxins يعني مثلا لما + +494 +00:37:26,920 --> 00:37:28,740 +بيقولوا كلوستروديوم بوترونيوم antitoxin + +495 +00:37:28,740 --> 00:37:33,120 +الكلوستروديوم بتتانى antitoxin يبقى لما يكون ال + +496 +00:37:33,120 --> 00:37:39,060 +antibody مخصص للتوكسين بنسميه antitoxin وغالبا ال + +497 +00:37:39,060 --> 00:37:46,200 +exotoxin بنقدر نتجلها antibodyالاندوتوكسين بيكوّل + +498 +00:37:46,200 --> 00:37:49,700 +لها انتبالي لكن مش effective .. مش as effective as + +499 +00:37:49,700 --> 00:37:54,360 +اللي هو الانتوتوكسين لمين؟ للإكسودوكسين بيعطيكوا + +500 +00:37:54,360 --> 00:37:59,840 +أمثلة كثيرة ال ديثيريا الفاكسين اللي احنا بناخده + +501 +00:37:59,840 --> 00:38:03,000 +.. بناخده .. بيعطيكوا اسم الفاكسين اللي بتحفظه .. + +502 +00:38:03,000 --> 00:38:06,080 +انا بكتبه على الصبورة عشان نضيفه من ضمن ال .. + +503 +00:38:18,580 --> 00:38:28,420 +دفتيريا، هاي دفتيريا، و هاي تيتانوس، لأ بيرتوز + +504 +00:38:28,420 --> 00:38:28,940 +بالأول + +505 +00:38:49,600 --> 00:38:53,280 +تمام؟ هذا التوكسين اللي هو ال DBT هو مشهور كل جوه + +506 +00:38:53,280 --> 00:38:57,420 +أخدته تمام؟ بيحمينا من ال Deuterium و من ال + +507 +00:38:57,420 --> 00:39:00,340 +Pertosis اللي يساعدكي زي اللي هو مين اللي هو + +508 +00:39:00,340 --> 00:39:04,140 +اتترست التلاتة بيشتركوا بإنهم ينتجوا بينتجوا + +509 +00:39:04,140 --> 00:39:08,520 +Exotoxin و بناخد ال Exotoxin تبعهم و بنحوله لما + +510 +00:39:08,520 --> 00:39:13,480 +يسمى إلى Toxide ماربعها المصطلحات العجب لها؟ لأ، + +511 +00:39:13,480 --> 00:39:21,340 +توكسين بتحول لإيه؟ Toxide لبنقول Oidإيش معناها؟ + +512 +00:39:21,340 --> 00:39:29,120 +Oid، metal، metalloid، alkaline، alkaloid، شبيه + +513 +00:39:29,120 --> 00:39:33,480 +بيه، شبيه بيه، metal، metalloid، إيش بقى؟ Toxin، + +514 +00:39:33,480 --> 00:39:35,700 +toxoid، خليكم معايا برنامج تنفذوا معايا في الجزئية + +515 +00:39:35,700 --> 00:39:42,230 +هذهإذا هالتوكسين إيه له صفتين بدنا نركز عليهم صفة + +516 +00:39:42,230 --> 00:39:46,590 +أولى ال toxicgenicity إيه ال toxicitygenicity؟ + +517 +00:39:46,590 --> 00:39:53,410 +السمية والصفة التانية ال antigenicity انه لو دخل + +518 +00:39:53,410 --> 00:39:57,170 +اسم الإنسان اسم الإنسان بيكوّله أسام مضادة احنا + +519 +00:39:57,170 --> 00:40:02,450 +مين اللي بيدينا؟ ال toxicgenic فبنروح و احنا .. + +520 +00:40:02,450 --> 00:40:09,020 +يعني لو نعملها بشكل خياليالطرفين هذا ال toxic part + +521 +00:40:09,020 --> 00:40:14,400 +و هذا ايش قلناه اللي هو ال antigenic part لو جسينا + +522 +00:40:14,400 --> 00:40:19,420 +ال toxigenic و خلينا بس ال antigenic ودخلنا هذا ال + +523 +00:40:19,420 --> 00:40:23,340 +antigen او هذا ال toxin الى دسم الحيوان، دسم + +524 +00:40:23,340 --> 00:40:27,140 +الحيوان بيكوله عسان مضاد دون ان يتأثر ان ال + +525 +00:40:27,140 --> 00:40:31,730 +toxigen استشمالها راحبناخد ال antibodies الجاهزة + +526 +00:40:31,730 --> 00:40:36,330 +هذه و بتصير أنها مصدر لو انزلجنا بدنا نعطي مريض + +527 +00:40:36,330 --> 00:40:40,750 +antibodies نعطيه ايه؟ امان؟ طيب احنا بدنا نضلنا + +528 +00:40:40,750 --> 00:40:43,330 +نصنع فيه الحيوانات و الحيوانات بدنا نصفي ال + +529 +00:40:43,330 --> 00:40:46,810 +antibodies و قد يبقى بقايا لو حقنها في إنسان كونه + +530 +00:40:46,810 --> 00:40:50,830 +غريب ممكن ال serum تبقى الحيوان يؤذي مين؟ الإنسان + +531 +00:40:50,830 --> 00:40:57,360 +فلماذا نقوم بإعطاء المريض نفسه تطيب محتوي علىعال + +532 +00:40:57,360 --> 00:41:01,460 +.. على الtoxoid اللي احنا هو يبارح عن تكسين جسنا + +533 +00:41:01,460 --> 00:41:05,680 +منه او خربنا فيه اللي هو ال toxicgenic part دون + +534 +00:41:05,680 --> 00:41:09,720 +انه اثر على مين؟ على ال antigenic part وسط الصورة + +535 +00:41:09,720 --> 00:41:13,840 +هاي ال toxoid، ال toxoid يبارح عن تكسين تم تدمير + +536 +00:41:13,840 --> 00:41:18,660 +ال toxicgenic side تبعه وهذا هو اللي انتوا أخدتوا + +537 +00:41:18,660 --> 00:41:21,440 +.. لما أخدتوا ال diphtheria و ال pertussis و ايه؟ + +538 +00:41:21,440 --> 00:41:27,340 +ال تتانوس اللي هو ال DPDإذا بنقدر نستنتج من اللوحة + +539 +00:41:27,340 --> 00:41:30,860 +دي اللي بدأنا نتكلم عنها استنتاج مهم جدا ان ال + +540 +00:41:30,860 --> 00:41:35,740 +exotoxins يمكن ال neutralization تبعتها بمين ب .. + +541 +00:41:35,740 --> 00:41:41,160 +ب .. بواسطة ال antitoxin هو يمكن لو انا اتناولت + +542 +00:41:41,160 --> 00:41:45,240 +الغذاء انا عندي توكسين الآن دخل مش بده يجيني بعد + +543 +00:41:45,240 --> 00:41:48,540 +سنة او سنتين الآن دخل فعشان انا عاجز اني معالجه ب + +544 +00:41:48,540 --> 00:41:49,580 +antitoxin + +545 +00:42:09,380 --> 00:42:13,880 +الشخص اللي أكل الطعام من فاسد هيكوسيرتوكسين، + +546 +00:42:13,880 --> 00:42:17,600 +فالناس جاهزة منها على هذا، يعني لإنه تلقائي بدون + +547 +00:42:17,600 --> 00:42:23,420 +إشكالجهاز المناعة إذا كان بيقدر يتعامل مع + +548 +00:42:23,420 --> 00:42:27,980 +المراكبات بشكل عام بيختلف، يعني انت لاحظ شغلة، في + +549 +00:42:27,980 --> 00:42:31,620 +سموم أقوى من سموم، مظبوط ولا لأ؟ لماذا سموم أقوى + +550 +00:42:31,620 --> 00:42:34,640 +من سموم؟ في سموم تشتغل على النار على طول وانتهى من + +551 +00:42:34,640 --> 00:42:39,920 +الموضوع، جهاز المناعة بالحاجش حتى يفكر، طبعا؟ وفي + +552 +00:42:39,920 --> 00:42:44,080 +توكسينز بتكون بطيقة ممكن تنبه جهاز المناعة + +553 +00:42:44,080 --> 00:42:48,600 +وبالتالي يكون في action لها، فأنا بعطي شغل تاني + +554 +00:42:49,470 --> 00:42:53,130 +بعطي muscle .. muscle يعني إيه؟ antitoxin جاهز برا + +555 +00:42:53,130 --> 00:42:58,970 +أو بعطي vaccine .. vaccine لمين؟ تخيل واحد زاكيتنا + +556 +00:42:58,970 --> 00:43:04,430 +ماعرفش أهتجاج في ال .. في خدنا نقول في العمل في + +557 +00:43:04,430 --> 00:43:07,710 +الميدان، مسامير وغيره وغيرته، يعني أقل أو أفضل من + +558 +00:43:07,710 --> 00:43:12,370 +الآخر، لكن في حياته معرض أن ينصف مصمار أو مصمرين + +559 +00:43:12,370 --> 00:43:18,220 +ولا أداة جرح من هنا أو من هناك، إيش بناعطيه؟عنطي + +560 +00:43:18,220 --> 00:43:22,600 +تكسيد اللي هو تكسين مضعف، فجسمنا بيكون كوّن أجسام + +561 +00:43:22,600 --> 00:43:27,760 +مضادة، فيما لو دخل التكسين حقا، جسمنا يقوم + +562 +00:43:27,760 --> 00:43:31,980 +بانجازله، بس لو حصل و أخدنا تكساء التكسين، + +563 +00:43:31,980 --> 00:43:35,600 +تناولنا، تمام؟ مافيش لنا حل إلا إنه ناخد عنطي + +564 +00:43:35,600 --> 00:43:36,360 +تكسيد + +565 +00:43:44,230 --> 00:43:48,350 +نجلع اللوحة المتعلقة بالاندوتوكسين و نطلع على + +566 +00:43:48,350 --> 00:43:54,810 +الرسم اللي موجودة هيصرف معروضة أمامكم بأشكال و + +567 +00:43:54,810 --> 00:43:58,850 +ألوان مختلفة منذ أن بدأنا بالحديث عن الاندوتوكسين + +568 +00:43:58,850 --> 00:44:03,010 +في النهاية هي مكوّنة من ثلاث أجزاء lipid-A, + +569 +00:44:03,350 --> 00:44:09,050 +corpulisicle, and oocyte chain الاندوتوكسين + +570 +00:44:09,050 --> 00:44:11,910 +هو ال lipid-Aمش مانع الحاجة .. مش مانع الحاجة ان + +571 +00:44:11,910 --> 00:44:15,510 +هو الأهم، بس احنا لإننا بنتكلم عن ال toxins تمام، + +572 +00:44:15,510 --> 00:44:24,750 +بنتكلم عن ال lipid A، ال lipid A اللي + +573 +00:44:24,750 --> 00:44:30,530 +سمناه كامرة لل endotoxinطبعا عشان يصيرله release + +574 +00:44:30,530 --> 00:44:34,410 +ال cell wall لازم يصيرله تحلل if it is required + +575 +00:44:34,410 --> 00:44:38,910 +for its release that the cell wall is lysed ممكن + +576 +00:44:38,910 --> 00:44:42,450 +نوصفه هذا ال .. ال .. ال .. ال library و ال .. انه + +577 +00:44:42,450 --> 00:44:46,490 +formaldehyde and heat resistant يعني فينا نوع من + +578 +00:44:46,490 --> 00:44:50,110 +ايه؟ من مقاومة لل formaldehyde ليش ال formaldehyde + +579 +00:44:50,110 --> 00:44:53,170 +بالذات؟لأن الformaldehyde هو اللي استخدمناه يا + +580 +00:44:53,170 --> 00:44:58,930 +بنات لتحويل ال toxin .. ال exotoxin ل toxoid يبقى + +581 +00:44:58,930 --> 00:45:04,850 +ال exotoxin حولت إلى toxoid بواسطة مين يبقى ال + +582 +00:45:04,850 --> 00:45:08,190 +formaldehyde عمل destruction لل toxic side لكن + +583 +00:45:08,190 --> 00:45:11,390 +للأسف لما حطيناها على ال endoxin ماقدرنا .. كنا + +584 +00:45:11,390 --> 00:45:15,590 +بننقل نفس الفكرة أننا نعمل toxoid من ال endoxin + +585 +00:45:15,590 --> 00:45:20,050 +ماقدرناماجدرناش تمام؟ فإن ال formal diet and heat + +586 +00:45:20,050 --> 00:45:23,550 +resistant poor antigen يعني و هذا كمان أحد نقاط + +587 +00:45:23,550 --> 00:45:29,150 +قوته و نقاط ضعفه نقاط قوة للبكتيريا إن الجسم لا + +588 +00:45:29,150 --> 00:45:33,690 +بيكوّلوش أجسام مضغطة بشكل قوي وبتالي مابتقدرش .. + +589 +00:45:33,690 --> 00:45:38,210 +مابتقدرش نقاوم البكتيريا من خلالها as a free + +590 +00:45:38,210 --> 00:45:41,790 +molecule هذا اليوم ماعرفش يعني .. المرة أنا ممكن + +591 +00:45:41,790 --> 00:45:44,650 +أبسلها و لا .. لو .. لو .. لو .. لو رابطنا .. لو + +592 +00:45:44,650 --> 00:45:49,270 +رابطنا ب .. بحدة تانية يعنيخلّيكم معاه، هي + +593 +00:45:49,270 --> 00:45:53,130 +المُركّب اللي هو ال intoxant، ك free molecule .. ك + +594 +00:45:53,130 --> 00:45:57,410 +free molecule، إيش فيه أنه فيه؟ فيه أنه poor + +595 +00:45:57,410 --> 00:46:00,630 +antigenicity، لكن أنا بنعت فيه في القلم المناع + +596 +00:46:00,630 --> 00:46:04,430 +اسمه adjuvant، adjuvant، لو ركّبنا هذا ال .. ال + +597 +00:46:04,430 --> 00:46:07,550 +molecule على ال adjuvant، بصير فيه نوع من ال .. + +598 +00:46:07,550 --> 00:46:11,210 +إيه؟ من ال response الأفضل، لكن لن يصل بأي حال من + +599 +00:46:11,210 --> 00:46:14,990 +الأحوال لل response تبع مين؟ تبع ال exotoxins اللي + +600 +00:46:14,990 --> 00:46:18,380 +شوفناها في التكسيراتنكمل ان شاء الله المرة القادمة + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..761900597d01b8f1681a6753a8915a53eace93ce --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/RZakKu7Qfl4_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 4689, "start": 20.65, "end": 46.89, "text": "بسم الله الرحمن الرحيم نكمل موضوع ال path genesis للبكتيريا و اليوم هتكلم عن جزء مهم جدا المتعلق بال invasion هذا الجزء يأتي بعد ال virulence ال factor الأساسي اللي درسناه اللي هو ال attachment أو ال adherence و درسنا مجموعة من العوامل تحت هذا ال factor و اليوم هتكلم ان شاء الله عن مجموعة من ال factors اللي بتساهم في ال invasion للبكتيريا", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 8717, 24793, 1211, 3714, 2407, 11242, 45367, 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90158, "start": 872.72, "end": 901.58, "text": "و بتضيف إليه ADP-ripose وعلى اللي بنسميها ADP-riposylation ADP اللي هو adenosine diphosphate الآن ضفنا ADP-ripose لمين؟ لل elongation factor 2 اللي كان active لما ضفناله بطل active لما بطل active يبقى الآن بطل يكون في دورة تصنيعة لبروتين", "tokens": [2407, 39894, 11242, 33911, 11933, 20292, 3224, 9135, 47, 12, 8400, 541, 4032, 3615, 23942, 13672, 1829, 44945, 38251, 1829, 11296, 9135, 47, 12, 8400, 329, 5088, 399, 9135, 47, 13672, 1829, 31439, 614, 20161, 533, 1026, 950, 16378, 473, 6024, 48506, 48812, 5172, 8315, 9135, 47, 12, 8400, 541, 32767, 9957, 22807, 24976, 40786, 399, 5952, 568, 13672, 1829, 25961, 4967, 5296, 15042, 48812, 5172, 8315, 43761, 4724, 9566, 1211, 4967, 5296, 15042, 4724, 9566, 1211, 4967, 7251, 3555, 4587, 7578, 6024, 48506, 4724, 9566, 1211, 7251, 30544, 8978, 11778, 13063, 3660, 6055, 9381, 22653, 27884, 5296, 26890, 35473, 9957], "avg_logprob": -0.2653186405990638, "compression_ratio": 1.6056338028169015, 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"end": 909.47, "word": " يعني", "probability": 0.88427734375}, {"start": 909.47, "end": 909.81, "word": " محكم", "probability": 0.9212239583333334}, {"start": 909.81, "end": 910.41, "word": " عليها", "probability": 0.982666015625}, {"start": 910.41, "end": 911.29, "word": " بالموت", "probability": 0.9612630208333334}, {"start": 911.29, "end": 911.99, "word": " لماذا", "probability": 0.6971028645833334}, {"start": 911.99, "end": 912.21, "word": " محكم", "probability": 0.96044921875}, {"start": 912.21, "end": 912.41, "word": " عليها", "probability": 0.989501953125}, {"start": 912.41, "end": 912.99, "word": " بالموت؟", "probability": 0.906494140625}, {"start": 912.99, "end": 913.49, "word": " كل", "probability": 0.9560546875}, {"start": 913.49, "end": 913.71, "word": " ال", "probability": 0.95556640625}, {"start": 913.71, "end": 914.39, "word": " enzymes", "probability": 0.97265625}, {"start": 914.39, "end": 914.75, "word": " اللي", "probability": 0.907470703125}, {"start": 914.75, "end": 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الخلية", "probability": 0.8513997395833334}, {"start": 921.05, "end": 921.81, "word": " بالموت", "probability": 0.7379557291666666}, {"start": 921.81, "end": 923.09, "word": " وبالتالي", "probability": 0.9263671875}, {"start": 923.09, "end": 923.45, "word": " اي", "probability": 0.752197265625}, {"start": 923.45, "end": 924.77, "word": " toxin", "probability": 0.736328125}], "temperature": 1.0}, {"id": 37, "seek": 93871, "start": 927.35, "end": 938.71, "text": "بيواجه تصنيع ال protein irreversibly بشكل غير قابل للعكس، غير قابل للعكس، غالبا .. غالبا بيؤدي لموت اللي هو ايه؟ الخلق", "tokens": [21292, 2407, 26108, 3224, 6055, 9381, 22653, 3615, 2423, 7944, 16014, 840, 3545, 4724, 8592, 28820, 32771, 13546, 12174, 16758, 1211, 24976, 3615, 4117, 3794, 12399, 32771, 13546, 12174, 16758, 1211, 24976, 3615, 4117, 3794, 12399, 32771, 6027, 3555, 995, 4386, 32771, 6027, 3555, 995, 4724, 1829, 33604, 16254, 32767, 35473, 13672, 1829, 31439, 1975, 1829, 3224, 22807, 33962, 1211, 4587], "avg_logprob": -0.23324093342788757, "compression_ratio": 1.4444444444444444, "no_speech_prob": 5.960464477539063e-08, "words": [{"start": 927.35, "end": 928.23, "word": "بيواجه", "probability": 0.5457763671875}, {"start": 928.23, "end": 928.61, "word": " تصنيع", "probability": 0.9644775390625}, {"start": 928.61, "end": 928.69, "word": " ال", "probability": 0.623046875}, {"start": 928.69, "end": 929.19, "word": " protein", "probability": 0.88037109375}, {"start": 929.19, "end": 931.91, "word": " irreversibly", "probability": 0.859375}, {"start": 931.91, "end": 932.63, "word": " بشكل", "probability": 0.8030598958333334}, {"start": 932.63, "end": 933.05, "word": " غير", "probability": 0.997314453125}, {"start": 933.05, "end": 933.45, "word": " قابل", "probability": 0.9944661458333334}, {"start": 933.45, "end": 934.03, "word": " للعكس،", "probability": 0.890625}, {"start": 934.03, "end": 934.49, "word": " غير", "probability": 0.84228515625}, {"start": 934.49, "end": 934.73, "word": " 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+الآخر المحاضرة تطرقنا لل doubling time أو زمان + +6 +00:00:39,820 --> 00:00:43,280 +الانقسام و simple binary equation اليوم هنتطرق لقصة + +7 +00:00:43,280 --> 00:00:47,900 +حياة البكتيريا كلنا كبشر إن قصص مختلفة و متنوعة + +8 +00:00:47,900 --> 00:00:55,020 +لكن تنتهي نهاية واحدة بالموت، القصة غير هيك البكتيريا + +9 +00:00:55,020 --> 00:00:59,580 +القصة اللي مرسومة على السبورة الآن هي القصة + +10 +00:00:59,580 --> 00:01:05,480 +المشتركة لكل أنواع البكتيريا عندما تقع البكتيريا + +11 +00:01:05,480 --> 00:01:11,060 +في غذاء أو في أي ديقة فيها مواد غذائية لا تبدأ + +12 +00:01:11,060 --> 00:01:16,160 +بالانقسام مباشرة وإنما تدرس + +13 +00:01:16,160 --> 00:01:22,330 +مكونات الوسط، وبالتالي تحتاج إلى فترة تعمل + +14 +00:01:22,330 --> 00:01:25,650 +adaptation بتجهز ال enzyme system تبعها وإحنا + +15 +00:01:25,650 --> 00:01:28,550 +حكينالكم في السابق قلنالكم مثلًا لو كان في جليكوز + +16 +00:01:28,550 --> 00:01:33,090 +ولاكتوز وكان فينا starch ويقدر على تناول هذه + +17 +00:01:33,090 --> 00:01:38,070 +المواد مثلها ده ستقوم بتجهيز ال enzyme system بحيث + +18 +00:01:38,070 --> 00:01:41,390 +إنه تقول إنه أنول أوفر للطاقة وفي دي الحالة هو + +19 +00:01:41,390 --> 00:01:45,340 +مين؟ الجليكوز، عند هذه النقطة لازم نجرب ونتكلم + +20 +00:01:45,340 --> 00:01:49,380 +بمصطلحين مهمين جدًا مش معمولين على السبورة، ال enzyme + +21 +00:01:49,380 --> 00:01:53,460 +ممكن نقسمهم إحنا إلى constitutive enzymes وكلمة + +22 +00:01:53,460 --> 00:01:56,320 +constitutive enzymes يعني enzymes that are + +23 +00:01:56,320 --> 00:02:02,760 +produced as long as the cell is growing or living + +24 +00:02:02,760 --> 00:02:08,120 +يعني بمعنى آخر إن الإنزيمات هتنتج طالما الخلية حية + +25 +00:02:08,120 --> 00:02:11,740 +يعني ما فيش عليها control ما فيش عليها control بمعنى + +26 +00:02:11,740 --> 00:02:17,940 +آخر دايمًا موجودة، بينما الـ induced enzymes they + +27 +00:02:17,940 --> 00:02:20,420 +will not be produced unless their substrate is + +28 +00:02:20,420 --> 00:02:24,500 +there يعني لما يصير فيه substrate لهم، فيه داعي، + +29 +00:02:24,500 --> 00:02:28,660 +فيه برتقال، بنجيب سكين من المطبخ، ما فيش برتقال، + +30 +00:02:28,660 --> 00:02:32,040 +ما نجبش خل السكين في المطبخ، وصورة هذا اسمه اسمه + +31 +00:02:32,040 --> 00:02:38,100 +induced enzyme مين الأوفر الطاقة قلناها؟ ال + +32 +00:02:38,100 --> 00:02:41,260 +constitutive لأنه موجود، it is already there يعني + +33 +00:02:41,260 --> 00:02:47,520 +سواء شئنا أم لا، هو موجود، وبتبدأ البكتيريا بالتغذية + +34 +00:02:47,520 --> 00:02:54,020 +لكن تنمو وتزداد حجمًا اللي قدامي في ال quick curve + +35 +00:02:54,020 --> 00:02:56,840 +هنا على السبورة اللي بتلاحظه بالخط الأحمر اللي هو + +36 +00:02:56,840 --> 00:03:01,880 +ال lag phase من zero time إلى time معين ما كان فيه + +37 +00:03:01,880 --> 00:03:04,540 +زيادة في عدد البكتيريا، ربما يكون فيه زيادة في + +38 +00:03:04,540 --> 00:03:11,560 +الحجم لكن ما فيه زيادة في العدد، بعد ذلك بدأت مرحلة + +39 +00:03:11,560 --> 00:03:15,740 +أخرى من الانقسام بالخط الأزرق أو اللون الأزرق صار + +40 +00:03:15,740 --> 00:03:22,760 +الانقسام 2 4 8 16 32 إيش بنسمي هذا؟ بنسميه زيادة + +41 +00:03:22,760 --> 00:03:27,300 +لوغاريتمية أو زيادة قصية exponential أو + +42 +00:03:27,300 --> 00:03:31,460 +logarithmic increase in number وبالتالي تتضاعف في + +43 +00:03:31,460 --> 00:03:36,800 +عددها اللي هو ال bacteria الآن إلى حد معين، البكتيريا + +44 +00:03:36,800 --> 00:03:41,400 +طبعًا الموجود ده في إناء مغلق، الإناء المغلق في كمية + +45 +00:03:41,400 --> 00:03:45,520 +محدودة من مين؟ من ال nutrients وبالتالي ال + +46 +00:03:45,520 --> 00:03:49,700 +nutrient بتبدأ بالنفاذ هذا واحد، رقم اثنين ال pH + +47 +00:03:49,700 --> 00:03:52,120 +بتبقى ال media as a result of the metabolic + +48 +00:03:52,120 --> 00:03:56,540 +processes of the bacteria will decrease، بنزل ال + +49 +00:03:56,540 --> 00:04:00,880 +pH ويصير acidic ال accumulation of waste products + +50 +00:04:00,880 --> 00:04:06,430 +بتزيد، اللي هو تراكم المخلفات تبع نمو البكتيريا، ال + +51 +00:04:06,430 --> 00:04:09,830 +Oxygen إذا كان البكتيريا بهذه ال Oxygen قد يقل في + +52 +00:04:09,830 --> 00:04:14,930 +الوسط لأن عندي مجموعة من العناصر بدأت إيه؟ خلّيني + +53 +00:04:14,930 --> 00:04:21,110 +أقول بطريقة متتالية للبكتيريا فصار عندي بعض البكتيريا + +54 +00:04:21,110 --> 00:04:25,230 +بتموت و البعض الآخر بينقسم ولو كنا في أي مجتمع في + +55 +00:04:25,230 --> 00:04:29,490 +الدنيا عدده فيه يساوي عدد المواليد لن يكون هناك + +56 +00:04:29,490 --> 00:04:34,090 +زيادة في العدد، انظروا إلى الخط الأخضر، الخط الأخضر + +57 +00:04:34,090 --> 00:04:38,590 +اللي مرسوم دلوقتي، بالمقابل في time فترة زمنية + +58 +00:04:38,590 --> 00:04:42,890 +هذه الفترة الزمنية بتقول إنه لا يوجد زيادة في العدد + +59 +00:04:42,890 --> 00:04:47,150 +لحد معينة مثلًا منها مرحلة الثبات أو stationary + +60 +00:04:47,150 --> 00:04:53,330 +phase كل ما ذكرناه من ظروف قلنا ساقت فأدت إلى ال + +61 +00:04:53,330 --> 00:04:59,390 +stationary phase عندما تزداد سوءًا بيصير عدده فيه + +62 +00:04:59,390 --> 00:05:04,540 +أكبر من عدد الانقسامات، أو بالظبط والآن بيبدأ العدد + +63 +00:05:04,540 --> 00:05:08,120 +أو ال number بتاعها to decline أو بنسمي decline + +64 +00:05:08,120 --> 00:05:11,960 +phase أو بنسمي death phase اللي هو اللون الزهري + +65 +00:05:11,960 --> 00:05:15,820 +هذا اللي موجود أمامك وإذا فينا أربع مراحل بنرجعهم + +66 +00:05:15,820 --> 00:05:19,160 +مع بعض على السطر اللي هم ال lag phase وهو لا + +67 +00:05:19,160 --> 00:05:21,760 +يوجد فيه increase إنما يوجد فيه مرحلة تحضير و + +68 +00:05:21,760 --> 00:05:25,280 +adaptation ال logarithmic phase وهو المرحلة الأكبر + +69 +00:05:25,280 --> 00:05:28,840 +في مرحلة النمو، ال stationary مرحلة ثبات ومرحلة + +70 +00:05:28,840 --> 00:05:33,300 +أخيرة اللي هي decline أو death phase، قصة حياة أي + +71 +00:05:33,300 --> 00:05:37,920 +بكتيريا غالبًا لا تتعدى هذه الخطوط، الفترة الزمنية + +72 +00:05:37,920 --> 00:05:42,180 +قد تطول قد تقصر لكن في الآخر الشكل اللي قدامنا و + +73 +00:05:42,180 --> 00:05:44,820 +المراحل اللي قدامنا هي اللي هتسود في هذه العملية + +74 +00:05:44,820 --> 00:05:50,040 +وسط الصورة، أرجو ده تبقوا معايا أهلا لنقطة كثير مهمة + +75 +00:05:50,040 --> 00:05:55,460 +بدنا نستفيد استفادة طبية فاكرين ال gram stain؟ كنا + +76 +00:05:55,460 --> 00:05:59,100 +نقول دي gram stain gram positive و gram negative، الفرق + +77 +00:05:59,100 --> 00:06:02,650 +بينهم مبني على ال cell wall، الأقل لما بيقول + +78 +00:06:02,650 --> 00:06:06,850 +البكتيريا بتموت أو في وضع سيء، بقى لكوا ال cell + +79 +00:06:06,850 --> 00:06:10,330 +wall هو الجدولة الأسمنتي، بده يقعد ثانوية لما + +80 +00:06:10,330 --> 00:06:14,270 +يتحلل؟ ولا يتحلل على طول؟ لذلك لو حوالينا نصبغ + +81 +00:06:14,270 --> 00:06:19,110 +بكتيريا بال gram stain وهي تموت، وهي تتحلل، لو + +82 +00:06:19,110 --> 00:06:21,930 +كانت ال gram positive هتبين ال gram negative لأن + +83 +00:06:21,930 --> 00:06:27,870 +السلوك تبعها بدأ يتحلل، إذا بنفهم أن أفضل منطقة و + +84 +00:06:27,870 --> 00:06:32,270 +أفضل جزئي نعمل فيها gram stain هي ال logarithmic + +85 +00:06:32,270 --> 00:06:34,850 +phase اللي هي معلنة باللون الأزرق على اللوحة اللي + +86 +00:06:34,850 --> 00:06:40,780 +هي أمامك، ليش؟ عشان كمان مرة البكتيريا بتكون في أوج + +87 +00:06:40,780 --> 00:06:43,820 +انقسامها، أفضل ما يكون ال cell wall بيكون في هذه + +88 +00:06:43,820 --> 00:06:47,300 +المنطقة، طبعًا في ال industrial microbiology أو في + +89 +00:06:47,300 --> 00:06:49,960 +ال microbiology الصناعية قد يكون ده كلام صحيح يعني + +90 +00:06:49,960 --> 00:06:53,280 +مش هذه الفترة هي المفضلة لدينا، ممكن تكون فترات + +91 +00:06:53,280 --> 00:06:57,320 +أخرى مفضلة لكن إحنا معنيين بهذه الفترة + +92 +00:07:00,890 --> 00:07:03,010 +صار عندنا فكرة عن ال growth نتكلم شوية عن ال + +93 +00:07:03,010 --> 00:07:05,830 +metabolism و ال metabolism إحنا كده مرة هنا + +94 +00:07:05,830 --> 00:07:09,230 +ما بنناقش biochemistry ما بنرجع كل ال biochemistry + +95 +00:07:09,230 --> 00:07:14,010 +وإنما بدنا نستغل بعض المعلومات في ال metabolism + +96 +00:07:14,010 --> 00:07:18,570 +حتى نستطيع من خلالها نتعرف على البكتيريا يعني ال + +97 +00:07:18,570 --> 00:07:21,910 +metabolism هنا بغرض مين؟ التعرف على البكتيريا + +98 +00:07:21,910 --> 00:07:26,320 +energy metabolism هنتكلم عنه شوية هنتكلم عن ال + +99 +00:07:26,320 --> 00:07:28,840 +medical important metabolic processes بمعنى + +100 +00:07:28,840 --> 00:07:32,940 +العمليات اللي لها علاقة بال medicine بال medical + +101 +00:07:32,940 --> 00:07:36,180 +microbiology تخيلوا بكتيريا as a waste product + +102 +00:07:36,180 --> 00:07:42,140 +بتنتج توكسين، أكيد التوكسين هذا هيأثر عليها تمام؟ وزي + +103 +00:07:42,140 --> 00:07:45,080 +أي metabolism في الدنيا إما anabolic process أو + +104 +00:07:45,080 --> 00:07:49,960 +catabolic process إما عمليات بناء أو عمليات، تعالوا + +105 +00:07:49,960 --> 00:07:53,720 +نشوف هذه اللوحة اللي أمامكم، اللوحة كثير جدًا مهمة + +106 +00:07:53,720 --> 00:07:58,440 +لنا واليوم يجب أن نؤكد على الفروقات الثلاث بين + +107 +00:07:58,440 --> 00:08:01,780 +الثلاث عناصر اللي موجودة أمامنا aerobic + +108 +00:08:01,780 --> 00:08:04,520 +respiration, anaerobic respiration and + +109 +00:08:04,520 --> 00:08:09,980 +fermentation، التلاتة هي عمليات قد تقوم بها + +110 +00:08:09,980 --> 00:08:14,840 +البكتيريا، وبالتالي نميز بينهم، التلاتة الأولى هي + +111 +00:08:14,840 --> 00:08:17,860 +الوحيدة اللي بتستهلك ال oxygen اللي هي aerobic + +112 +00:08:17,860 --> 00:08:23,250 +respiration، بينما ال anaerobic respiration و ال + +113 +00:08:23,250 --> 00:08:27,150 +fermentation بتموا في غياب ال oxygen، طب كيف بنا + +114 +00:08:27,150 --> 00:08:30,810 +نميز بينهم من ناحية biochemistry؟ الأولى اللي هي + +115 +00:08:30,810 --> 00:08:34,710 +ال aerobic respiration، ال oxygen is the final + +116 +00:08:34,710 --> 00:08:38,190 +electron acceptor، + +117 +00:08:38,190 --> 00:08:39,110 +أظن واضحة النقطة هذه + +118 +00:08:43,420 --> 00:08:48,340 +استفادة من الكربوهيدرات بتتم في وجود الأكسجين one + +119 +00:08:48,340 --> 00:08:54,260 +molecule will produce 36 ATPs، نيجي الآن لل + +120 +00:08:54,260 --> 00:08:57,260 +Anaerobic Respiration و ال fermentation، كيف بنا نميز + +121 +00:08:57,260 --> 00:09:00,560 +بينهم، وخاصة إنه في ورية شبه إنه بتتموا في غياب + +122 +00:09:00,560 --> 00:09:04,720 +الأكسجين، ال Anaerobic Respiration ال final + +123 +00:09:04,720 --> 00:09:08,380 +electron acceptor بيكون inorganic compound مركب + +124 +00:09:08,380 --> 00:09:12,960 +غير عضوي غير الأكسجين، Inorganic compound غير ال + +125 +00:09:12,960 --> 00:09:16,900 +Oxygen بينما ال fermentation بيكون ال final + +126 +00:09:16,900 --> 00:09:20,960 +electron acceptor هو organic compound وصلت الصورة + +127 +00:09:20,960 --> 00:09:25,740 +Oxygen للأولى، Inorganic compound للتانية، ثلاثة، + +128 +00:09:25,740 --> 00:09:29,620 +أكثر واحد more efficient أو the most efficient + +129 +00:09:29,620 --> 00:09:32,300 +اللي هي aerobic respiration، the least efficient + +130 +00:09:32,300 --> 00:09:38,710 +اللي هي fermentation هي الأقل إنتاجًا للطاقة كيف + +131 +00:09:38,710 --> 00:09:41,650 +نستغل وهذه اللوحة اللي أمامنا وكتير مهمة نضلنا + +132 +00:09:41,650 --> 00:09:46,110 +مركزين عليها طوال الوقت حتى نستطيع أن نفهم ماذا + +133 +00:09:46,110 --> 00:09:51,890 +يعني بهذه اللوحة احنا في الـ microbiology قد يظن + +134 +00:09:51,890 --> 00:09:54,710 +البعض أنه كون الميكروبات دقيقة و احنا ممكن + +135 +00:09:54,710 --> 00:09:59,010 +المحاضرة مش المواد اللي جابلها تحدثنا عن استخدامات + +136 +00:09:59,010 --> 00:10:02,550 +الميكروسكوبات يمكن احنا أقل الناس اللي بنستخدم في + +137 +00:10:02,550 --> 00:10:07,620 +مجال الميكروسكوب، ليش؟ طورنا أنظمة .. أنظمة الـ + +138 +00:10:07,620 --> 00:10:10,800 +detection بالتغيير في الألوان، بنوعها على مين؟ + +139 +00:10:10,800 --> 00:10:13,320 +بننوعها بتغيير في الـ pH فمثلا الصورة اللي قدامكوا + +140 +00:10:13,320 --> 00:10:17,620 +هذه اللون الأحمر هو اللون الأصلي للوسط صنع + +141 +00:10:17,620 --> 00:10:21,500 +ببكتيريا فتحول إلى لون الأصفر، ليش تحول إلى لون + +142 +00:10:21,500 --> 00:10:25,560 +الأصفر؟ لأن البكتيريا عملت fermentation أو عملت + +143 +00:10:25,560 --> 00:10:30,460 +metabolism فخفضت الـ pH واحنا حاطين pH indicator، + +144 +00:10:30,460 --> 00:10:34,450 +تمام؟ فإيش يعني pH Indicator؟ يعني مركب يتغير + +145 +00:10:34,450 --> 00:10:38,930 +لونه بتغير الـ pH فتغير من اللون الأحمر لللون + +146 +00:10:38,930 --> 00:10:41,590 +الأصفر عرفنا إنه العينة أو ال .. أو ال .. + +147 +00:10:41,590 --> 00:10:45,830 +البكتيريا positive، إيش يعني positive؟ يعني استطاعت + +148 +00:10:45,830 --> 00:10:49,690 +أن تقوم بالمهمة، إيش المهمة؟ to ferment أو to + +149 +00:10:49,690 --> 00:10:52,530 +metabolize على سبيل المثال الـ dextrose أو الـ + +150 +00:10:52,530 --> 00:10:56,970 +glucose، طيب التنتين صفر صح؟ اللي في الصورة التنتين + +151 +00:10:56,970 --> 00:11:00,630 +صفر في فرق بينهم؟ في واحدة طالعة غاز، شايفينها؟ + +152 +00:11:04,720 --> 00:11:08,160 +انطلقت منها غازي بعد الحكي أنه في كمان شغل عملناها + +153 +00:11:08,160 --> 00:11:11,140 +detection في الـ metabolism أنه صار fermentation + +154 +00:11:11,140 --> 00:11:15,640 +positive or negative، كيف عرفناها؟ بتغيير اللون في + +155 +00:11:15,640 --> 00:11:18,360 +كمان شغل تاني ممكن نعرفها أنه fermentation with + +156 +00:11:18,360 --> 00:11:22,280 +gas ولا fermentation with no gas، هاي كمان صفة + +157 +00:11:22,280 --> 00:11:26,160 +جديدة، يعني بدل الفحص سرنانيا محصانة بدل الصفة + +158 +00:11:26,160 --> 00:11:31,250 +سرناندي صفة ثانية، مثال آخر على الـ metabolic + +159 +00:11:31,250 --> 00:11:36,150 +process اللي بتساعدنا في عملية اللي هو التعرف على + +160 +00:11:36,150 --> 00:11:41,930 +البكتيريا، فحص اسمه methyl red، الـ + +161 +00:11:41,930 --> 00:11:46,810 +methyl red هو عبارة عن برضه pH indicator، وسمى الفحص + +162 +00:11:46,810 --> 00:11:51,310 +باسم الـ pH Indicator المستخدم، نظرا ليش؟ لأنه + +163 +00:11:51,310 --> 00:11:55,510 +بنضيف الـ Methyl Red مش مباشرة مع الميديا وإنما + +164 +00:11:55,510 --> 00:11:58,690 +بعد نمو البكتيريا، يعني بمعنى آخر نزرع البكتيريا في + +165 +00:11:58,690 --> 00:12:04,030 +الميديا تمام؟ فيها جلوكوز وبعد مرور 24 ساعة بنضيف + +166 +00:12:04,030 --> 00:12:09,730 +عليها الـ Methyl Red، إذا تحول الوسط إلى اللون + +167 +00:12:09,730 --> 00:12:13,720 +الأحمر كما نرى في هذه الصورة، ده بيكون positive، ماما + +168 +00:12:13,720 --> 00:12:19,460 +اللون الأحمر أنه الـ pH تبع الـ media وصل لأقل من + +169 +00:12:19,460 --> 00:12:23,800 +أربعة وهم هتدرسوه بالتفصيل في المعمل إن شاء الله + +170 +00:12:23,800 --> 00:12:28,060 +وهتشتغلوا شغل عملي بإذن الله، لكن في الآخر ميزات + +171 +00:12:28,060 --> 00:12:31,700 +بين بكتيريا methyl red positive وأخرى methyl red + +172 +00:12:31,700 --> 00:12:35,280 +negative، اللون الأحمر positive واللون الأصفر + +173 +00:12:35,280 --> 00:12:35,740 +negative + +174 +00:12:38,500 --> 00:12:42,080 +الـ Voges-Proskauer هو نوع آخر من الفحوصات المعتمدة + +175 +00:12:42,080 --> 00:12:45,380 +على الـ metabolic processes، هناك بعض البكتيريا + +176 +00:12:45,380 --> 00:12:51,880 +عندها metabolic pathway اسمه 2,3-butylene glycol + +177 +00:12:51,880 --> 00:12:59,960 +أحد الـ precursors المميزة، مركب اسمه أسيتوين وليس + +178 +00:12:59,960 --> 00:13:04,220 +أسيتون، في فرق، أسيتوين هو اسم تاني + +179 +00:13:04,220 --> 00:13:07,520 +أسيتالميثالكاربينول، برضه هدرسه في المعمل إن شاء + +180 +00:13:07,520 --> 00:13:12,790 +الله، هذا المركب إن موجود فبمؤشر على وجود الـ 2,3 + +181 +00:13:12,790 --> 00:13:17,110 +-butylene glycol fermentation pathway، إذا لم يوجد + +182 +00:13:17,110 --> 00:13:20,850 +فمعناه الحاجة أن البكتيريا لا تمتلك هذا الـ pathway + +183 +00:13:20,850 --> 00:13:25,650 +بعد ما بننمي البكتيريا بنضيف two compounds يعني + +184 +00:13:25,650 --> 00:13:29,990 +two detectors، إذا تحولت الأنبوبة إلى لون الأحمر + +185 +00:13:29,990 --> 00:13:35,410 +بصي، فيما تحولت إلى لون الأحمر negative، استنتجت من خلال + +186 +00:13:35,410 --> 00:13:36,330 +فحص + +187 +00:13:39,410 --> 00:13:43,050 +مؤشر على وجود metabolic pathway، وأنا متكلم عن مين + +188 +00:13:43,050 --> 00:13:46,790 +احنا الآن في المحاضرة هاي؟ متكلم عن الـ bacterial + +189 +00:13:46,790 --> 00:13:51,830 +physiology، عن الـ growth and metabolism، فاستغلنا الـ + +190 +00:13:51,830 --> 00:13:55,570 +Metabolism في التفريق بين أنواع الـ micro .. الـ + +191 +00:13:55,570 --> 00:13:58,270 +microorganism وأول محاضرة .. إذا بترجعوا + +192 +00:13:58,270 --> 00:14:01,530 +المحاضرة الأولى هتتذكروا إن أنا قلتلكوا معلومة جدا + +193 +00:14:01,530 --> 00:14:04,490 +إن ما يميز الـ microorganism هو الـ metabolic + +194 +00:14:04,490 --> 00:14:08,970 +biodiversity، التنوع في الـ metabolism، بكتيريا تتغذى + +195 +00:14:08,970 --> 00:14:12,450 +على الجلوكوز، أخرى على الجلوكوز زائد الـ lactose، أخرى + +196 +00:14:12,450 --> 00:14:17,310 +على الجلوكوز زائد الـ maltose وهكذا، أنواع مختلفة من + +197 +00:14:17,310 --> 00:14:20,730 +الـ substrate تستغل من قبل أنواع مختلفة من الـ micro + +198 +00:14:20,730 --> 00:14:26,630 +organism، حصل رابعا والصورة اللي أمامكوا واضحة كتير + +199 +00:14:26,630 --> 00:14:31,670 +أنه في لونين أزرق وأخضر، الـ original color للأنبوبة + +200 +00:14:31,670 --> 00:14:34,510 +هذه هو اللون الأخضر، الـ original color هو اللون + +201 +00:14:34,510 --> 00:14:38,830 +الأخضر، إذا تحول إلى اللون الأزرق فالفحص positive + +202 +00:14:38,830 --> 00:14:43,140 +هقولكوا كيف بتحول، هذا الفحص معتمد على قدرة + +203 +00:14:43,140 --> 00:14:47,840 +البكتيريا عليها تستغل الـ sodium citrate كمصدر + +204 +00:14:47,840 --> 00:14:51,520 +كربوني وحيد، كمصدر كربوني وحيد، مش كل البكتيريا + +205 +00:14:51,520 --> 00:14:55,100 +عندها القدرة هذه، أنواع قليلة من الميكروبات عندها + +206 +00:14:55,100 --> 00:14:58,900 +القدرة هذه، إذا بقدر أميز هذه الأنواع عن بعض + +207 +00:14:58,900 --> 00:15:02,580 +الأنواع الأخرى اللي ما عندهاش هذه القدرة، بحط + +208 +00:15:02,580 --> 00:15:06,560 +أنبوبة فيها sodium citrate وفيها pH indicator + +209 +00:15:06,560 --> 00:15:12,140 +اسمه bromthymol blue، إذا البكتيريا استهلكت .. + +210 +00:15:12,140 --> 00:15:17,060 +استهلكت .. ال .. الـ sodium citrate هتستهلك معاه + +211 +00:15:17,060 --> 00:15:22,800 +مركب عضوي آخر بيرفع الـ pH إلى أعلى، أعلى يعني + +212 +00:15:22,800 --> 00:15:30,170 +alkaline، لما يرتفع الـ pH إلى alkaline بيتحول اللون + +213 +00:15:30,170 --> 00:15:32,790 +الاسم bromethymol، الـ pH Indicator اللي اسمه + +214 +00:15:32,790 --> 00:15:36,810 +bromthymol blue إلى من أخضر إلى أزرق، وابتدا اللي + +215 +00:15:36,810 --> 00:15:40,310 +بيقول الفحص هي positive، إذا بقى كما هو الفحص + +216 +00:15:40,310 --> 00:15:44,430 +negative، هذا كان فحص اللي عارفينه الآن الـ + +217 +00:15:44,430 --> 00:15:46,570 +carbohydrate fermentation test، خدوا بالكم مش فحص + +218 +00:15:46,570 --> 00:15:51,980 +واحد، الـ carbohydrate fermentation test مش بس لأ، مش + +219 +00:15:51,980 --> 00:15:56,500 +بس كمان هيك، لكل carbohydrate بقدر أعمل فحص، يعني + +220 +00:15:56,500 --> 00:15:59,820 +بعمل للجلوكوز، لللاكتوز، للسكروز، للمالتوز، + +221 +00:15:59,820 --> 00:16:04,460 +لل سوربيتول، لمجموعة كبيرة من السكريات، وكلها بقدر + +222 +00:16:04,460 --> 00:16:08,240 +آخد منها الصفتين، الـ positive fermentation زائد اللي هو + +223 +00:16:08,240 --> 00:16:13,710 +الـ gas production، الثلاث فحوصات التانية كانوا methyl + +224 +00:16:13,710 --> 00:16:17,910 +red، Voges-Proskauer و citrate، هنتقل إلى الرابع في + +225 +00:16:17,910 --> 00:16:21,230 +السلسلة اللي هم مجموعين مع بعض اللي اسمه Indole test + +226 +00:16:29,830 --> 00:16:35,290 +الآن فحص الاندول هو وسيلة للكشف عن مقدرة البكتيريا + +227 +00:16:35,290 --> 00:16:40,070 +على إنتاج إنزيم اسمه tryptophanase، بيكسر مين هذا؟ + +228 +00:16:40,070 --> 00:16:43,790 +tryptophan، تكسير لـ tryptophan بينتج عنه مركب + +229 +00:16:43,790 --> 00:16:47,950 +اسمه اندول، هذا لأن دول بتتم الكشف عنه بإضافة برضه + +230 +00:16:47,950 --> 00:16:50,670 +بعض المركبات الكيميائية باسمها Kovac's reagent + +231 +00:16:50,670 --> 00:16:56,550 +إذا تكونت الـ red ring حلقة الحمراء هذه، إذا تكونت + +232 +00:16:56,550 --> 00:17:01,890 +فالفحص positive، يبقى قدامي هنا بصمة لأنه فيه red + +233 +00:17:01,890 --> 00:17:05,470 +ring، الـ red ring نتيجة على أنه المادة اللي بنضيفها + +234 +00:17:05,470 --> 00:17:10,630 +فيها كحول بتستخلص الـ indole والمادة الأخرى الملونة + +235 +00:17:10,630 --> 00:17:14,550 +وبالتالي بتستخلصه و بترفعه وإن على السطح فبتكون + +236 +00:17:14,550 --> 00:17:17,570 +red ring على السطح، في الـ negative إيش فيها نهاية + +237 +00:17:17,570 --> 00:17:23,050 +في الـ negative؟ زي ما شايفين ما فيش لون، لون الـ + +238 +00:17:23,050 --> 00:17:26,270 +reagent فقط اللي هتنضف فيها، الأنبوبة الأولى اللي + +239 +00:17:26,270 --> 00:17:30,430 +على الجهة هاي هي أنبوبة غير مزروعة أساسا للمقارنة + +240 +00:17:30,430 --> 00:17:33,190 +عشان نقدر نشوف أنه صار نمو ولا ما صار نمو في + +241 +00:17:33,190 --> 00:17:36,470 +الأنبوبة الأخرى، أنا كان فحص صاروا الآن أربعة غير + +242 +00:17:36,470 --> 00:17:39,410 +الأولى، تبقى من الأولى نهرج بيكربوا دي، صار عندي + +243 +00:17:39,410 --> 00:17:43,770 +Indole، Methyl red، Voges-Proskauer و Citrate، أنا + +244 +00:17:43,770 --> 00:17:46,750 +الترتيب اللي في اللوحة مش هو الترتيب اللي أنا + +245 +00:17:46,750 --> 00:17:49,950 +بدهيه، الترتيب اللي أنا ذكرته كمرات Indole + +246 +00:17:56,130 --> 00:17:59,130 +بشكل سريع يا بنات بنرجع للأربع فحوصات اللي + +247 +00:17:59,130 --> 00:18:03,030 +شفناهم ونطلع كلها تحت صورة إيش مكتوبة أو الصورة + +248 +00:18:03,030 --> 00:18:06,370 +الأولى اللي هي E.coli وإنتيروباكتر + +249 +00:18:06,370 --> 00:18:10,250 +positive and negative، الـ default E.coli الـ + +250 +00:18:10,250 --> 00:18:11,730 +negative، الإنتيروباكتر positive + +251 +00:18:26,040 --> 00:18:30,980 +يبقى أنا عندي أربع فحوصات قارنت بين the two organisms, + +252 +00:18:31,200 --> 00:18:34,560 +two similar organisms وقت أنتِ طلعوا positive + +253 +00:18:34,560 --> 00:18:39,440 +لِفحص الشريحة كلها طلعت positive لأن دول ميزرد و + +254 +00:18:39,440 --> 00:18:42,320 +negative للتانين للتانين الباجين و ال enterobacter + +255 +00:18:42,320 --> 00:18:45,660 +negative للأولانين التانين و positive للتانين + +256 +00:18:45,660 --> 00:18:50,780 +التانين طيب ألم يكن يكفينا فحص واحد، نقول سيترات + +257 +00:18:50,780 --> 00:18:54,980 +بسيط و سيترات negative؟ خذ القاعدة اللي لازم احنا + +258 +00:18:54,980 --> 00:18:58,220 +يعني نكتبها بحروف من هذا في ال diagnostic + +259 +00:18:58,220 --> 00:19:01,560 +microbiology أو في ال medical the more tests you + +260 +00:19:01,560 --> 00:19:05,040 +use the more accurate is your diagnosis يعني كل ما + +261 +00:19:05,040 --> 00:19:09,720 +زاد عدد الفحوصات اللي بستخدمهم كل ما زاد دقة عملية + +262 +00:19:09,720 --> 00:19:10,900 +اللي هو التعريف + +263 +00:19:14,280 --> 00:19:16,340 +الـ differentiation فاكرينه لما كنت أقول لكم + +264 +00:19:16,340 --> 00:19:20,300 +سالمونيلا وشيجيلا و مميز منهم بال motility غالبا + +265 +00:19:20,300 --> 00:19:25,800 +نعم هو فحص مميز لكن لا نعتمد عليه لوحده كفحص واحد + +266 +00:19:25,800 --> 00:19:31,160 +و لا نقارن معاه فحوصات أخرى نرجع و نكمل ال .. ال .. + +267 +00:19:31,160 --> 00:19:36,400 +ال MVIC ال MVIC اللي قدامكم هو رموز لأربع فحوصات + +268 +00:19:36,400 --> 00:19:41,670 +ال I in doll و ال M مثيل Red و ال V أو ال VPبوجرس + +269 +00:19:41,670 --> 00:19:45,270 +بروس كوار و ال C سيتيطر على ال I just to make it + +270 +00:19:45,270 --> 00:19:49,730 +قابل للنطق أن نقول Invict تمام؟ و شايفين في الجدول + +271 +00:19:49,730 --> 00:19:55,010 +التحت أن الشريحة كولاي positive positive negative + +272 +00:19:55,010 --> 00:19:58,390 +negative بينما ال enterobacter negative negative + +273 +00:19:58,390 --> 00:20:02,990 +positive positive أعتقد أن هذه المجموعة من + +274 +00:20:02,990 --> 00:20:07,310 +الفحوصات أثبتت لنا beyond reasonable doubt يعني + +275 +00:20:07,310 --> 00:20:11,980 +بدون أدنى شك أنه ال metabolic processes ال + +276 +00:20:11,980 --> 00:20:17,340 +biochemistry التابعة للبكتيريا مهمة جدا في عملية + +277 +00:20:17,340 --> 00:20:22,060 +تمييز البكتيريا أعطيكم توضيح كامل من ألفه إلى يائه + +278 +00:20:22,060 --> 00:20:27,580 +تكرر ال grams تان؟ قلنا جد عظيم ال grams تان؟ + +279 +00:20:27,580 --> 00:20:30,640 +بشكل كبير قلنا عنه عظيم قلنا عنه إنه بميز بين + +280 +00:20:30,640 --> 00:20:34,160 +مجموعتين كبيرتين gram بسيط و gram negative روحنا لل + +281 +00:20:34,160 --> 00:20:36,460 +gram negative احنا اللي قاعدين نتكلم عن بكتيريا + +282 +00:20:36,460 --> 00:20:39,340 +مجهولة عزلناها لما عملنا gram stain طلعت gram + +283 +00:20:39,340 --> 00:20:45,260 +negative طلعت شكلها على جنبها بسلايد فصار عندي أنا أربع + +284 +00:20:45,260 --> 00:20:51,420 +من أربع ايش هم الأربعة؟ gram positive coexi و gram + +285 +00:20:51,420 --> 00:20:54,640 +negative coexi gram positive بسلايد و gram negative + +286 +00:20:54,640 --> 00:20:59,660 +بسلايد يبقى صار عندي أنا واحد من أربع أربع هذا الربع + +287 +00:20:59,660 --> 00:21:04,080 +بتبقى أجسام كمان أجسام عندي أربع أصناف مش للحفظ + +288 +00:21:04,080 --> 00:21:09,000 +الكلام اللي بحكيه لأن لكن للتوضيح أنتروباكترياسي + +289 +00:21:09,000 --> 00:21:13,340 +family كبيرة ضخمة سودوموناس اسينيتوباكتر و + +290 +00:21:13,340 --> 00:21:17,080 +فيبريو أربع أصناف طلعت البكتيريا تبعتي تابعة + +291 +00:21:17,080 --> 00:21:21,720 +لعائلة اسمها انتيروباكترياسي الانتيروباكترياسي + +292 +00:21:21,720 --> 00:21:27,270 +فيها 200 اسم كلهم بيمتلكوا نفس الصفة اللي أنا قلت + +293 +00:21:27,270 --> 00:21:30,910 +عشان أنا مش عارف انتيروباكترياسي يعني تمتلك كلهم + +294 +00:21:30,910 --> 00:21:33,850 +الصفات بيمتلكوا نفس الصفة أنهم يكونوا نفس العائلة + +295 +00:21:33,850 --> 00:21:37,910 +لكن جوا أسماء مختلفة بدي أميزهم عن بعض من ضمن ال + +296 +00:21:37,910 --> 00:21:42,750 +انتيروباكترياسي اشيريشيا كولاي و انتروباكتر بينقسم + +297 +00:21:42,750 --> 00:21:45,610 +الانتيروباكترياسي إلى lactose fermenter و من ال + +298 +00:21:45,610 --> 00:21:48,950 +lactose fermenter هدول طلعوا ضمن مين ضمن ال + +299 +00:21:48,950 --> 00:21:52,370 +lactose fermenter شوفوا قد ايش أوجه التشابه بينهم + +300 +00:21:52,370 --> 00:21:59,510 +ترجعوا بالجلوكوز fermenter، جليكوز fermenter، اوكسيدايز + +301 +00:21:59,510 --> 00:22:03,430 +negative، فاكولتاتف أناقركم كل هذه صفات مشتركة بين + +302 +00:22:03,430 --> 00:22:09,050 +الانتروباكتر و بين الاشريشيا كولاي طب أوجه تشابهم + +303 +00:22:09,050 --> 00:22:12,770 +متعددة و كثيرة يبقى في حاجة لإن احنا نميزهم عن + +304 +00:22:12,770 --> 00:22:16,890 +بعضهم من البعض، مين اللي بيميزهم؟ ال biochemical + +305 +00:22:16,890 --> 00:22:20,570 +reactions لم نستطع نميزهم لا بجرامس تان ولا + +306 +00:22:20,570 --> 00:22:23,350 +بتقنية مختلفة أخرى ميزناهم بال biochemical + +307 +00:22:23,350 --> 00:22:26,950 +reactions لذلك تصبح مهمة جدا من ما عمل لكم هذا الفصل + +308 +00:22:26,950 --> 00:22:32,330 +هيكون مهتم بإجراءها بفهم و إجراء هذه الفحوصات اللي + +309 +00:22:32,330 --> 00:22:36,490 +هنستخدمها لاحقا في ال diagnostic microbial بتعرف + +310 +00:22:36,490 --> 00:22:42,520 +على ال micro organism في كمان بعض الفحوصات الأخرى + +311 +00:22:42,520 --> 00:22:45,060 +الكثيرة المتنوعة أكثر من ما أنا صادف في هذه + +312 +00:22:45,060 --> 00:22:49,160 +المحاضرة لكن أحدها حاجة اسمها hydrogen sulfide + +313 +00:22:49,160 --> 00:22:56,700 +production H2S الـ H2S غاز عادي من اللون لكن + +314 +00:22:56,700 --> 00:23:02,590 +رائحته كريهة تعرفوها وسموها رائحة البيض الفاسد اللي + +315 +00:23:02,590 --> 00:23:05,470 +بنشم بعض الأحيان في الشوارع اللي يكون فيها مجاري + +316 +00:23:05,470 --> 00:23:09,790 +راكدة هادر و لونها أسود طبعا أنا قاعد بقول عادي من + +317 +00:23:09,790 --> 00:23:15,170 +اللون هو صح؟ عادي من اللون لكن لو تفاعل مع الحديد + +318 +00:23:15,170 --> 00:23:18,990 +و الحديد موجود بكثرة في ال .. في المياه العادية، + +319 +00:23:18,990 --> 00:23:22,770 +الأشياء المصدية و الأمور هذه حديد فبيرتبط مع + +320 +00:23:22,770 --> 00:23:26,770 +الحديد بيكون لون أسود، بيكون لون أسود فاحنا برضه كيف + +321 +00:23:26,770 --> 00:23:30,590 +بنكشف أن البكتيريا بتنتج أو بتنتجوش؟ بنحط في ال + +322 +00:23:30,590 --> 00:23:35,780 +media حديد مركبات حديد فإذا الغاز طلع بيتحول الوسط + +323 +00:23:35,780 --> 00:23:39,740 +اللامين إلى اللامض الأزرق في فحص ثاني اسمه urease + +324 +00:23:39,740 --> 00:23:44,620 +لو دخلنا دورات حمامات مش منظمة فترة طويلة بنشم + +325 +00:23:44,620 --> 00:23:46,920 +رائحة الأمونيا من بعيد رائحة كريهة الأمونيا + +326 +00:23:46,920 --> 00:23:51,420 +بالنسبة لنا الأمونيا هذه ناتجة عن أن بكتيريا + +327 +00:23:51,420 --> 00:23:54,620 +موجودة في الدورات كسرت ال urea الموجودة في ال + +328 +00:23:54,620 --> 00:23:59,390 +urine وطلعت منها الأمونيا نفس الفكرة بنقدر نستغل + +329 +00:23:59,390 --> 00:24:02,990 +اللي احنا في المعمل بنجيب قنبوبة و فيها يوريا و + +330 +00:24:02,990 --> 00:24:07,510 +بنزرع البكتيريا و إذا البكتيريا كسرت ال urea + +331 +00:24:07,510 --> 00:24:10,710 +هتحولها لأمون ال ammonia alkaline حطينا في الوسط + +332 +00:24:10,710 --> 00:24:15,190 +pH indicator تحول اللون إلى لون آخر و بنقول + +333 +00:24:15,190 --> 00:24:20,330 +positive أو negative في هذه الحالة إذا كل أو معظم + +334 +00:24:20,330 --> 00:24:23,930 +الفحوصات اللي مرّ علينا هل شفناها تحت ال + +335 +00:24:23,930 --> 00:24:28,030 +microscope ولا طلعنا عليها تغيير ألوان تغيير ألوان + +336 +00:24:28,030 --> 00:24:31,350 +دي دايمة هي طبعا هتكون مفضلة بالنسبة لكم لأن + +337 +00:24:31,350 --> 00:24:34,830 +ألوانها كثير جذابة اللي بفكر أو بالأحرى اللي في + +338 +00:24:34,830 --> 00:24:38,170 +حاجات الباقية هو ال chemical طبعا احنا كمان هنتطور + +339 +00:24:38,170 --> 00:24:42,310 +ليه؟ لأن احنا شايفين الأنابيب ما أكبرهم اللي بنحط + +340 +00:24:42,310 --> 00:24:47,270 +فيهم كبير لما نصل لمرحلة معينة نفهم ال .. ال .. ال + +341 +00:24:47,270 --> 00:24:50,310 +.. الأنظمة كلها ال .. ال .. الفحوصات البرمجية + +342 +00:24:50,310 --> 00:24:57,090 +هنسخطهم نزغرهم و نحط 20 فحص في سلسلة واحدة + +343 +00:24:57,090 --> 00:25:01,290 +مثل ال API system هنشوف برضه نماذج مقتصرة أخرى + +344 +00:25:01,290 --> 00:25:02,470 +غير ال API system + +345 +00:25:05,080 --> 00:25:09,100 +احنا اتكلمنا على منتجات استخدمناها في التعرف على + +346 +00:25:09,100 --> 00:25:12,080 +ال microbenz تعرفنا أنا اتكلم على منتجات أخرى سيئة + +347 +00:25:12,080 --> 00:25:16,660 +الذكر زي ال pyrogen ال pyrogen this is a fever + +348 +00:25:16,660 --> 00:25:21,020 +producing substance synthesized by بكتيريا يعني + +349 +00:25:21,020 --> 00:25:25,840 +fever producing يعني لو دخل المركب إن فيريزمنا + +350 +00:25:25,840 --> 00:25:30,880 +بيصير إن حرارته مرتفعة حرارة مرتفعة خذوا بالكم مش + +351 +00:25:30,880 --> 00:25:34,540 +ضروري البكتيريا تكون جوه يكفي يكون ال pyrogen جوه + +352 +00:25:35,520 --> 00:25:40,160 +بتركزوا معايا في هذه المعلومة المهمة بتعرفوا ال + +353 +00:25:40,160 --> 00:25:43,660 +stringات؟ شفتوا ما هي ال stringات؟ بتكون أظن مسكرة + +354 +00:25:43,660 --> 00:25:49,580 +و معقمة، تمام؟ ال string هذه اللي تخيل إنها كانت + +355 +00:25:49,580 --> 00:25:54,320 +مغموسة ب إشريشيا كولاي و سكرناها و بعدين عقبناها + +356 +00:25:54,320 --> 00:25:57,580 +أو برنامج إشريشيا كولاي نأخذ و نركب حالة نعرفها + +357 +00:25:57,580 --> 00:26:00,400 +Staphylococcus aureus تعرفوا Staphylococcus + +358 +00:26:00,400 --> 00:26:06,820 +aureus؟ لأن البكتيريا ماتت بعد ما عقبناها لكن لم + +359 +00:26:06,820 --> 00:26:10,420 +تختفي عن كوكب الأرض، لم تختفي من السرنجة، ضايق + +360 +00:26:10,420 --> 00:26:14,520 +المركبات منها، بقايا منها، زي و احنا لما .. لما + +361 +00:26:14,520 --> 00:26:19,420 +بنموت، نختفيش فجأة، بنتحلل تدريجيا ولا لا، نفس + +362 +00:26:19,420 --> 00:26:24,460 +الشيء البكتيريا، ما تختفيش، تتحلل من ضمن المركبات + +363 +00:26:24,460 --> 00:26:27,460 +اللي بتتركها البكتيريا، مركبات زي الـpeptidoglycan + +364 +00:26:27,460 --> 00:26:32,940 +زي الـteichoic acid، فاكرين الـteichoic acid؟ في + +365 +00:26:32,940 --> 00:26:36,420 +الـspores، dipicolonic acid الـ lipopolysaccharide + +366 +00:26:36,420 --> 00:26:41,160 +ما بتدمرش بسرعة أو بسهولة هذه المركبات وإن كان + +367 +00:26:41,160 --> 00:26:44,680 +البكتيريا ميتة لو استخدمنا سيرنج هذا حقنّا مركب + +368 +00:26:44,680 --> 00:26:48,900 +معين مثلا antibiotic أو محلول في جسم الإنسان وداخل + +369 +00:26:48,900 --> 00:26:53,300 +جسم الإنسان حيصير عنده fever على الرغم من عدم وجود + +370 +00:26:53,300 --> 00:26:58,120 +بكتيريا وإنما مركبات إذا بعض المنتجات اللي بتعمل + +371 +00:26:58,120 --> 00:27:00,420 +fever بيسميها pyrogen + +372 +00:27:03,960 --> 00:27:07,420 +النظافة قبل التعقيم + +373 +00:27:13,950 --> 00:27:17,630 +لازم ننظف بالأول و بعدين نعقمه لأنه لابد أن أبدأ + +374 +00:27:17,630 --> 00:27:21,370 +ببيئة خالية أصلا من ال micro organism للأسف هذه + +375 +00:27:21,370 --> 00:27:26,190 +الثقافة قد تكون غير موجودة عند المصنعين في مصانعهم + +376 +00:27:26,190 --> 00:27:29,650 +الأخضر، خاصة المحلية، برا لأ، برا في عندهم أنظمة + +377 +00:27:29,650 --> 00:27:33,750 +مراقبة بتخلي الثقافة غصب عنهم موجودة، بس عندنا لأ، + +378 +00:27:33,750 --> 00:27:38,330 +أقولك ما لأ، همروا الغذاء بعملية طبخ أو طهي + +379 +00:27:38,330 --> 00:27:42,470 +يعني حرارة مرتفعة يعني قتل للميكرو أورجانيزم أوك + +380 +00:27:42,470 --> 00:27:45,890 +أنت قتلت للميكرو أورجانيزم لكن مركباتها ومنطلقاتها + +381 +00:27:45,890 --> 00:27:51,470 +ما زالت موجودة بس رح نحكي عنها هون Exotoxin و invasive + +382 +00:27:51,470 --> 00:27:58,010 +enzymes Exotoxins يعني سموم تنتجها البكتيريا + +383 +00:27:58,010 --> 00:28:00,450 +while alive عكس مين + +384 +00:28:08,150 --> 00:28:10,830 +الـ lipopolysaccharide اللي هو الـ lipid A اللي + +385 +00:28:10,830 --> 00:28:13,490 +موجود في الـ lipopolysaccharide في الـ outer + +386 +00:28:13,490 --> 00:28:16,250 +membrane تبع ال gram negative بكتيريا اعتقدوا أن + +387 +00:28:16,250 --> 00:28:19,470 +هذا ما فيش أي خلاف كل gram negative بكتيريا تحتوي + +388 +00:28:19,470 --> 00:28:23,570 +على اندوتوكسين لكن بعض ال gram negative بكتيريا أو + +389 +00:28:23,570 --> 00:28:27,090 +بعض ال gram بصي تنتج أيضاً إكزوتوكسين يعني + +390 +00:28:27,090 --> 00:28:30,250 +البكتيريا بتكون حية و بتنتج هذا التوكسين و أعطيكم + +391 +00:28:30,250 --> 00:28:35,280 +مثال كثير سهل و بسيط بتعرفوا التيتانوس؟ حكينا عنه + +392 +00:28:35,280 --> 00:28:38,220 +مرة ال Clostridium Tetani أو Clostridium Tetani + +393 +00:28:38,220 --> 00:28:43,360 +لما بغرز المصمار الشخص في قدمه على سبيل المثال ال + +394 +00:28:43,360 --> 00:28:47,480 +bacteria بتدخل جوا ال spores و بتبدأ تتكاثر في بعض + +395 +00:28:47,480 --> 00:28:51,620 +الظروف المعينة و بتنقسم في نفس المكان لا تغادر + +396 +00:28:51,620 --> 00:28:56,980 +المكان اللي دخلت منه و بتنتج ال toxin تبعها في نفس + +397 +00:28:56,980 --> 00:29:01,930 +المكان التوكسين لأنه مركبات صغيرة و will diffuse + +398 +00:29:01,930 --> 00:29:05,510 +و في الآخر بيصل للدم و بيروح لل central nervous + +399 +00:29:05,510 --> 00:29:09,630 +system البكتيريا الموجودة localized .. localized + +400 +00:29:09,630 --> 00:29:15,730 +يعني في مكان محدود بينما .. بينما المنتج تبعها + +401 +00:29:15,730 --> 00:29:19,350 +اللي هو ال exotoxin خرج منها و خرج من نطاق اللي + +402 +00:29:19,350 --> 00:29:21,630 +localized صار systemic و التأثير تبعه بيكون + +403 +00:29:21,630 --> 00:29:22,350 +systemic + +404 +00:29:32,510 --> 00:29:37,210 +مبتسم بشكل دائم، مبتسم بشكل دائم هو يعني هذا مؤشر + +405 +00:29:37,210 --> 00:29:40,950 +على أنه ليس كل ابتسامة هي تعبّر عن السعادة، ربما + +406 +00:29:40,950 --> 00:29:47,270 +يكون عنده تيتانوس، تمام؟ فهذه نقطة كثير مهمة الآن + +407 +00:29:47,270 --> 00:29:51,790 +كلمة invasive enzymes خليكم معايا أنا فاندي خلية + +408 +00:29:51,790 --> 00:29:56,770 +و خلية مرتبطين في بعض في نسيج الآن الارتباط هذا + +409 +00:29:56,770 --> 00:30:03,040 +بيجعل من الأنسجة عائق أمام البكتيريا للاختراق، + +410 +00:30:03,040 --> 00:30:07,900 +لا تستطيع إنها تغزو مناطق بعيدة عن مكان تواجدها، + +411 +00:30:07,900 --> 00:30:12,060 +فاجتمع البكتيريا تنتج enzymes زي مثلا الكولاجينيز + +412 +00:30:12,060 --> 00:30:18,420 +والإيلاستيز، الكولاجينيز والإيلاستيز بيدوبوا + +413 +00:30:19,490 --> 00:30:23,410 +الفواصل ما بين الخلايا وبالتالي بيسمح للبكتيريا + +414 +00:30:23,410 --> 00:30:27,970 +أنها تغزو الأنسجة فمسموها invasive enzymes يعني + +415 +00:30:27,970 --> 00:30:32,090 +إنزيمات تساعد البكتيريا في غزو الأنسجة ووصل + +416 +00:30:32,090 --> 00:30:34,870 +الصورة أن فيها اندريجز توكسينز و أخذنا مثلا لها + +417 +00:30:34,870 --> 00:30:38,550 +اللي استخدم تيتانوس توكسين أو تيتانوسبازمين و اتكلمنا + +418 +00:30:38,550 --> 00:30:42,590 +عن invasive enzyme و أخذنا مثل كولاجينيز and اللي + +419 +00:30:42,590 --> 00:30:48,180 +هو الإيلاستيز نجي لل pigment بعض المركبات بعض + +420 +00:30:48,180 --> 00:30:52,000 +البكتيريا ألوانها جميلة ألوانها جميلة تفيل + +421 +00:30:52,000 --> 00:30:55,480 +البكتيريا الخضراء هذه خضراء مزرقة أعتقد اللون يكون + +422 +00:30:55,480 --> 00:31:00,120 +أخضر مزرق بكتيريا اسمها Pseudomonas aeruginosa + +423 +00:31:00,120 --> 00:31:04,820 +Pseudomonas aeruginosa بتنتج pigment و اللي على + +424 +00:31:04,820 --> 00:31:08,020 +الجهة الثانية اللون الأحمر هذه البكتيريا اسمها + +425 +00:31:08,020 --> 00:31:14,960 +Serratia marcescens لون أحمر الآن بتلاحظوا ملاحظة + +426 +00:31:14,960 --> 00:31:19,920 +نكية أن في اللون الأحمر اللون فقط موجود في + +427 +00:31:19,920 --> 00:31:24,740 +المستعمرات مش موجود في الطبق بينما في اللون + +428 +00:31:24,740 --> 00:31:30,650 +الثانية منتشر في المستعمرات وموجود في وين؟ في الطبق + +429 +00:31:30,650 --> 00:31:35,390 +دلوقت عايش على أن water soluble و water insoluble + +430 +00:31:35,390 --> 00:31:40,050 +مين ال insoluble الحمرة اللي تأثيراتها مارس سنس + +431 +00:31:40,050 --> 00:31:46,290 +بينما الـ Pseudomonas aeruginosa water soluble هل هناك + +432 +00:31:46,290 --> 00:31:51,090 +فوائد لل pigment ولا ما فيش فوائد البكيرة بتنتج بس + +433 +00:31:51,090 --> 00:31:56,090 +عشان تتلون يعني تصبغ حاجة تشوفوا أحد الفوائد اللي + +434 +00:31:56,090 --> 00:31:59,010 +أخذنا أفهى عن Pseudomonas aeruginosa أنها بتنتج في + +435 +00:31:59,010 --> 00:32:04,960 +الوسط تنتشر في الوسط و بترتبط في بعض المركبات زي ال + +436 +00:32:04,960 --> 00:32:09,060 +iron بيصير ال iron هذا bound فاكرين ال bound + +437 +00:32:09,060 --> 00:32:12,880 +water؟ هل كان .. هل .. هل البكتيريا بتستفيد من ال + +438 +00:32:12,880 --> 00:32:17,520 +bound water؟ وإن ما بتستفيدش؟ الآن ال bound iron + +439 +00:32:17,520 --> 00:32:21,180 +اللي في الوسط هذا لو كان فيه بكتيريا أخرى في + +440 +00:32:21,180 --> 00:32:26,580 +المنطقة الآن لحظة هي مثلا في المنطقة هذه فيه + +441 +00:32:26,580 --> 00:32:30,420 +بكتيريا لكن هنا ما فيش بكتيريا تخيلوا في استافيل + +442 +00:32:30,420 --> 00:32:33,640 +الكوكتاس اوريس في المنطقة هذه، الاستافيلو الكوكاس + +443 +00:32:33,640 --> 00:32:38,420 +اوريس بدها ايرون، فيه بدهم ايرون، لكن الـ iron هذا + +444 +00:32:38,420 --> 00:32:42,840 +مرتبط، مرتبط مين؟ بالصبغة تبع ال .. ال .. ال .. ال + +445 +00:32:42,840 --> 00:32:43,340 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +446 +00:32:43,340 --> 00:32:43,500 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +447 +00:32:43,500 --> 00:32:44,460 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +448 +00:32:44,460 --> 00:32:45,000 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +449 +00:32:45,000 --> 00:32:47,180 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +450 +00:32:47,180 --> 00:32:47,200 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +451 +00:32:47,200 --> 00:32:47,360 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +452 +00:32:47,360 --> 00:32:51,810 +.. ال .. ال .. ال ..يعني بتجعل الحديد غير متاح + +453 +00:32:51,810 --> 00:32:56,970 +للآخرين، وسيلة من وسائل التنافس المخيفة، يعني + +454 +00:32:56,970 --> 00:33:01,390 +البشر ما بيعرفوش يستخدم .. ينكرون هذا المقر أو .. + +455 +00:33:01,390 --> 00:33:04,230 +في زمان اتحادين بيعرفوا أكثر، بيتعلموا من + +456 +00:33:04,230 --> 00:33:07,410 +البكتيريا، + +457 +00:33:07,410 --> 00:33:10,370 +يعني الآن زي واحد بيدي بالظبط مش بعمل .. بخش في + +458 +00:33:10,370 --> 00:33:14,160 +قاعة زي هذه و بروح بحط ورقة محجوز، محجوز، محجوز، + +459 +00:33:14,160 --> 00:33:17,620 +محجوز، تمام؟ كل اللي بيجوا بيلاقوا الورقة المحجوز + +460 +00:33:17,620 --> 00:33:21,220 +بيسحبوا عليهم إيه و بيطلعوا إلى الخارج، بيقدروا + +461 +00:33:21,220 --> 00:33:23,940 +يستفيدوا من الكراسي، معناه الكراسي موجودة، لكن أنا + +462 +00:33:23,940 --> 00:33:26,420 +وقت ما بدي باجي و بجد على كرسي، أي كرسي من الكراسي + +463 +00:33:26,420 --> 00:33:29,200 +هذا، فإذا البكتيريا وصلت لل iron، بتاخدوه و + +464 +00:33:29,200 --> 00:33:34,550 +بتستخدموه هنا البيجمنت في كل ميكروفيلة فائدة مختلفة + +465 +00:33:34,550 --> 00:33:39,530 +عن الآخر في بعض منها أساسي في الـ virulence، أساسي + +466 +00:33:39,530 --> 00:33:42,470 +في الـ virulence، يعني virulence فاكرة، المهم أنه + +467 +00:33:42,470 --> 00:33:47,010 +فيه pigments و فيه ألوان للبكتيريا سوداموناس + +468 +00:33:47,010 --> 00:33:50,570 +إرجينوزا في ألوان أصفر و في أخضر و في أزرق و ما + +469 +00:33:50,570 --> 00:33:56,810 +بينهما أصفر مخضر أخضر مصفر أزرق مخضر أخضر مصفر لكن + +470 +00:33:56,810 --> 00:33:59,910 +هاي ال pigments بتاعي هاي السيرات اللون الأحمر + +471 +00:33:59,910 --> 00:34:03,190 +القاني اللي بيشبه اللي حد كده بيسموه tomato juice + +472 +00:34:03,190 --> 00:34:07,690 +يعني عصير الطماطم اللون بتاعها إذا في عندي مجموعة + +473 +00:34:07,690 --> 00:34:11,750 +من المنتجات اللي بتنتجها الوقت إكسدوكسين invasive + +474 +00:34:11,750 --> 00:34:13,950 +enzymes وهي كان عنها pigments + +475 +00:34:16,250 --> 00:34:20,290 +أنتيبايوتكس تنتجها البكتيريا هو ال .. ال .. ال .. ال + +476 +00:34:20,290 --> 00:34:21,930 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +477 +00:34:21,930 --> 00:34:21,950 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +478 +00:34:21,950 --> 00:34:23,450 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +479 +00:34:23,450 --> 00:34:23,730 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +480 +00:34:23,730 --> 00:34:24,110 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +481 +00:34:24,110 --> 00:34:33,010 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +482 +00:34:33,010 --> 00:34:33,650 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +483 +00:34:33,650 --> 00:34:33,690 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +484 +00:34:33,690 --> 00:34:33,750 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +485 +00:34:33,750 --> 00:34:33,850 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +486 +00:34:33,850 --> 00:34:33,970 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +487 +00:34:34,020 --> 00:34:37,780 +البنسلين من فطر يعني أقصدوا أن البكتيريا .. + +488 +00:34:37,780 --> 00:34:40,060 +البكتيريا بتنتج .. أو بعض البكتيريا .. ال + +489 +00:34:40,060 --> 00:34:44,100 +actinomycetes أشهر مجموعة حكينا عنها مبرح .. لما + +490 +00:34:44,100 --> 00:34:47,920 +اتكلمنا عن المطر، تمام؟ و أن الرائحة اللي بنشمها + +491 +00:34:47,920 --> 00:34:51,600 +أول ما بتشت الدنيا ناتجة عن ال actinomycetes، ال + +492 +00:34:51,600 --> 00:34:54,860 +actinomycetes من أشهر منتجات ال antibiotic في + +493 +00:34:54,860 --> 00:35:01,140 +الدنيا، تمام؟ Bacteriocins، هناك بعض المركبات لا + +494 +00:35:01,140 --> 00:35:05,540 +ترتقي نسميها Antibiotics، لكن نقدر نصفها + +495 +00:35:05,540 --> 00:35:09,540 +Antibiotic-like substances، يعني مركبات تشبه الـ + +496 +00:35:09,540 --> 00:35:11,780 +Antibiotic، إيش يعني ممكن تشبهها؟ تشبهها + +497 +00:35:11,780 --> 00:35:17,440 +بالـ actions، تنتجها البكتيريا في أمعاقنا، حتى تحد + +498 +00:35:17,440 --> 00:35:21,630 +من نمو الآخر إحنا لو .. لو اتكلمنا عن البكتيريا + +499 +00:35:21,630 --> 00:35:25,730 +اللي موجودة في معي الإنسان هي عبارة عن دوار أغمى + +500 +00:35:25,730 --> 00:35:30,270 +.. دوار أغمى تتنافس فيما بينها بأسلحة متنوعة + +501 +00:35:30,270 --> 00:35:34,150 +ومختلفة فعشان كده فيه اتزان لإن كل واحد عارف قوة + +502 +00:35:34,150 --> 00:35:37,830 +الآخر .. عارف قوة الآخر من ضمن الأسلحة المستخدمة + +503 +00:35:37,830 --> 00:35:41,770 +في التوازن هو الـ bacteriocenes اللي هنا بتشبه مين + +504 +00:35:41,770 --> 00:35:45,470 +الـ antibiotics و هناك بعض البكتيريا بتنتج vitamins + +505 +00:35:45,470 --> 00:35:48,860 +و احنا قولنا لكم كل واحد فوائد الـ normal flora في + +506 +00:35:48,860 --> 00:35:52,060 +الأمعاء أو الـ normal microbiota هو إنتاج بعض أنواع + +507 +00:35:52,060 --> 00:35:57,080 +من اللي هو الـ vitamin z و الـ vitamin k يبقى فيه + +508 +00:35:57,080 --> 00:36:02,700 +منتجات ضارة فيه منتجات جيدة فيه virulence factors + +509 +00:36:02,700 --> 00:36:06,040 +فيه toxins فيه أشياء مستغللة احنا في التعرف على الـ + +510 +00:36:06,040 --> 00:36:11,540 +micro organisms الآن + +511 +00:36:11,540 --> 00:36:15,610 +نيجي لاستغلال كل هذه المعلومات اللي دراسناها من + +512 +00:36:15,610 --> 00:36:21,230 +أول المحاضرة اليوم ومبارح إلى توظيف هذه المعلومات + +513 +00:36:21,230 --> 00:36:26,850 +في المعمل artificial cultivation of bacteria يعني + +514 +00:36:26,850 --> 00:36:30,050 +الآن بدنا نمي البكتيريا بشكل صناعي طب ما هي بتنمو + +515 +00:36:30,050 --> 00:36:33,550 +في ال .. في البيئة و بتتكاثر في البيئة لكن أنا بدي + +516 +00:36:33,550 --> 00:36:37,390 +نميها بشكل صناعي طبعا تنمية البكتيريا أكيد ليست + +517 +00:36:37,390 --> 00:36:43,430 +رفاهية وإنما لغرض أو لمجموعة أغراض من مجموعة + +518 +00:36:43,430 --> 00:36:46,830 +الأغراض هذه أن نتعرف على البكتيريا لا أستطيع أن + +519 +00:36:46,830 --> 00:36:50,350 +أتعرف على البكتيريا بشكل دقيق بيسموها الـ golden + +520 +00:36:50,350 --> 00:36:55,310 +standard يعني أفضل وسيلة للتعرف على البكتيريا هو + +521 +00:36:55,310 --> 00:37:00,510 +تنميتها in pure culture بمزرعة نقية ومن ثم دراسة + +522 +00:37:00,510 --> 00:37:03,570 +خصائصها إيه كانت دراسة خصائصها gram positive ولا + +523 +00:37:03,570 --> 00:37:06,050 +gram negative اندول بوزتف ولا اندول بوزتف اللي هو الـ + +524 +00:37:06,050 --> 00:37:10,710 +biochemical test اللي هتعلموهم في المعملي يُطلق على + +525 +00:37:10,710 --> 00:37:15,390 +الوسط المستخدم في تنمية البكتيريا Medium و جمعها + +526 +00:37:15,390 --> 00:37:20,630 +Media أو وسائط Media Medium وسط It's a liquid or + +527 +00:37:20,630 --> 00:37:25,490 +solid mixture خلطة سائلة أو صلبة designed to + +528 +00:37:25,490 --> 00:37:30,090 +support the growth of microorganisms usually + +529 +00:37:30,090 --> 00:37:33,590 +called broth of liquid إذا كانت سائلة بنسميها broth + +530 +00:37:33,590 --> 00:37:38,720 +و broth معناها مرق شوربة يعني تمام؟ و usually called + +531 +00:37:38,720 --> 00:37:42,880 +agar if solid بنسميها agar لإن أصلا نضيف مادة + +532 +00:37:42,880 --> 00:37:46,980 +اسمها agar agar تمام بتخليها بالضبط solid إذن في + +533 +00:37:46,980 --> 00:37:51,480 +عندي شكلين أساسيين في الوسط الزراعي liquid and + +534 +00:37:57,010 --> 00:38:02,110 +اللي هو يعني بنضيف كمية أجار، بالضبط، حتى يجعل + +535 +00:38:02,110 --> 00:38:07,290 +قوامه يعني semi-solid، مش solid ولا هو liquid لغة + +536 +00:38:07,290 --> 00:38:11,110 +بواحد، وهو حقيقة فحص الـ motility، فحص الـ motility، + +537 +00:38:11,110 --> 00:38:16,920 +هالكلة و أنا لوحدي فعلا، طيب الأوساط الزراعية هناك + +538 +00:38:16,920 --> 00:38:20,280 +تصنيفات إلا بشكل كبير يعني .. يعني .. يعني .. خدنا + +539 +00:38:20,280 --> 00:38:24,580 +نقول نجا نصنفها بأكثر من وسيلة من ضمن الوسائل + +540 +00:38:24,580 --> 00:38:28,580 +according to basic ingredients المحتوى الـ .. الـ .. + +541 +00:38:28,580 --> 00:38:31,260 +الـ .. الـ .. أو المحتويات اللي موجودة في الوساطة + +542 +00:38:31,260 --> 00:38:34,880 +فمثلا عندنا حاجة Minimal Essential Growth Medium + +543 +00:38:34,880 --> 00:38:38,860 +ومن اسمه Minimal Essential Growth Medium + +544 +00:38:43,820 --> 00:38:48,340 +بمعنى آخر، الحد الأدنى من المطلوبات للنمو، يعني + +545 +00:38:48,340 --> 00:38:51,620 +ينتلق هذا الوسط، الحد الأدنى من المطلوبات للنمو، + +546 +00:38:51,620 --> 00:38:57,740 +مش الـ enrichment main يعني، + +547 +00:38:57,740 --> 00:39:03,660 +بده شرح هذا، نركز مع بعض، فاكرين لما اتكلمنا عن + +548 +00:39:03,660 --> 00:39:07,280 +البكتيريا وسط الـ wall، كنا البكتيريا اللي بتنزل في + +549 +00:39:07,280 --> 00:39:13,110 +الحمام، بتنزل في الماء الحلوة، أمام؟ و الميه العذبة + +550 +00:39:13,110 --> 00:39:17,690 +مافيش فيها ملح بكميات كافية تعمل balance بين الـ .. + +551 +00:39:17,690 --> 00:39:20,390 +بالضغط الأسموزي اللي تبقى على الخلية و الـ .. و + +552 +00:39:20,390 --> 00:39:24,090 +البيئة وبالتالي بيكون البكتيريا under stress وفي + +553 +00:39:24,090 --> 00:39:28,230 +البيئة البكتيريا معظم الأوقات بتكون under stress + +554 +00:39:28,230 --> 00:39:32,110 +ومدام under stress لما أنا أبتدأ .. أقولكوا بلاش + +555 +00:39:32,110 --> 00:39:34,310 +في رسم الإنسان .. لما أنا أبتدأ أقل من رسم الإنسان + +556 +00:39:34,310 --> 00:39:37,310 +مش في حرب دائرة أساسا احنا بين .. بين البكتيريا و + +557 +00:39:37,310 --> 00:39:40,190 +بين الـ immune system فممكن البكتيريا تكون under + +558 +00:39:40,190 --> 00:39:40,830 +stress + +559 +00:39:43,680 --> 00:39:47,680 +طبعا باللغة العربية هتكون كلمة مجروحة لكن مقصود + +560 +00:39:47,680 --> 00:39:53,780 +فيها أنه مش في صحتها يعني مش بأفضل صورها عشان أنا + +561 +00:39:53,780 --> 00:40:00,720 +أنميها لازم أحطها في وسط غني جدا و يساعدها على + +562 +00:40:00,720 --> 00:40:06,220 +الاستجمام من المصائب اللي ألمت فيها و من الـ stress + +563 +00:40:06,220 --> 00:40:09,260 +اللي كان متوقع له و هذا الوسط غالبا بيسميه احنا إيه + +564 +00:40:09,260 --> 00:40:14,240 +enrichment mainly في المستقبل هنعيد تعريفه بما + +565 +00:40:14,240 --> 00:40:17,920 +يتلاقى مع موضوعات أخرى لكن هذا لأن الحد الأدنى اللي + +566 +00:40:17,920 --> 00:40:23,040 +هنفهمه أنها أوساط غنية جدا تستخدم لإنعاش بعض + +567 +00:40:23,040 --> 00:40:29,260 +الميكروبات اللي هي بتكون under stress نيجي للجزء + +568 +00:40:29,260 --> 00:40:32,180 +أو النوع الثالث اللي هو الـ selective medium + +569 +00:40:35,200 --> 00:40:41,000 +عبارة عن وسط استخدمناه لانتقاء نوع معين من + +570 +00:40:41,000 --> 00:40:46,160 +البكتريا كيف إن انتقاء نوع معين؟ تخيلوا لو أنا بدي + +571 +00:40:46,160 --> 00:40:51,380 +أحكم على غذاء أنه صالح للاستخدام أو غير صالح + +572 +00:40:51,380 --> 00:40:55,800 +للاستخدام والمواصفة الفلسطينية للأغذية أو لهذا + +573 +00:40:55,800 --> 00:40:59,920 +الغذاء بتقول يجب أن يكون هناك أكثر من مئة + +574 +00:40:59,920 --> 00:41:06,090 +staphylococcus aureus طيب يبقى أنا الآن مضطر أعد + +575 +00:41:06,090 --> 00:41:10,810 +فقط مين؟ طب هو يمكن يكون ما يكون بعد أخرى مسموح + +576 +00:41:10,810 --> 00:41:14,770 +فيها، أنا ما بديها، المواصفة بتقول أعد اللي جداش + +577 +00:41:14,770 --> 00:41:22,330 +فيه استافيلوكوكاس or yes فلازم أصمم وسط لا ينمي + +578 +00:41:22,330 --> 00:41:24,650 +إلا استافيلوكوكاس or yes + +579 +00:41:28,120 --> 00:41:32,820 +يعني باختصار كله لأننا عارفه بنقول هو وسط يستخدم + +580 +00:41:32,820 --> 00:41:38,780 +للنمو والانتقاء للبكتيريا نيجي الأخيرة اللي هو الـ + +581 +00:41:38,780 --> 00:41:42,220 +differential أعطاني مرة معناها مصطلح differential + +582 +00:41:42,220 --> 00:41:47,360 +الـ differential معناها تميز يميز صح الـ + +583 +00:41:47,360 --> 00:41:52,240 +differential media هي وسط تـ differentiate between + +584 +00:41:52,240 --> 00:41:54,940 +two similar organisms أو بتعطيكم مثال + +585 +00:42:03,350 --> 00:42:07,390 +السلمونيلا والإيشريشيا كولاي التنتين انترو + +586 +00:42:07,390 --> 00:42:11,230 +بكترياسي التنتين انترو بكترياسي والتنتين مكون + +587 +00:42:11,230 --> 00:42:13,990 +موجودين في الـ intestine يعني موجودين في عينة الـ + +588 +00:42:13,990 --> 00:42:17,830 +stool عينة البراز إذا أكرمكم الله إذا بدي أعزل + +589 +00:42:17,830 --> 00:42:21,630 +السلمونيلا من الوسط بروح بحطها في وسط اسمه الماكنكي + +590 +00:42:21,630 --> 00:42:26,670 +الماكنكي بيميز لي بين two types of microorganisms + +591 +00:42:26,670 --> 00:42:32,020 +بين lactose fermenter و لاكتوز فرمنتر واحدة أعطاها + +592 +00:42:32,020 --> 00:42:36,440 +لون أحمر وواحدة ما أعطاهاش لون خالص دي في وسط عمل + +593 +00:42:36,440 --> 00:42:41,580 +differentiation عمل differentiation فدي بقى لكوا + +594 +00:42:41,580 --> 00:42:48,300 +رقم Z و D من أعظم الإنجازات في مختبرات الـ + +595 +00:42:48,300 --> 00:42:53,040 +microbiology ومن كتر ما هم مهمين غالبا بيلاقيش + +596 +00:42:53,040 --> 00:42:56,460 +selective لحاله في الـ medical و differential لحاله + +597 +00:42:56,460 --> 00:43:00,870 +فبيلاقي الوسط نفسه بيمتلك إيه الصفتين بنسميه + +598 +00:43:00,870 --> 00:43:05,190 +selective differential نرجع للمقنكي المقنكي + +599 +00:43:05,190 --> 00:43:09,630 +selective بحيث أنه وضع فيه بعض المركبات بحيث لا + +600 +00:43:09,630 --> 00:43:12,070 +ينمي الـ gram booster فهو selective ده الـ gram + +601 +00:43:12,070 --> 00:43:16,270 +negative وإذا استطاع أن يميز بين lactose fermenter + +602 +00:43:16,270 --> 00:43:18,710 +و لا lactose fermenter فهو differential + +603 +00:43:23,950 --> 00:43:26,690 +كمان الوضع ده اللي استخدمناه لعدل Staphylococcus + +604 +00:43:26,690 --> 00:43:30,070 +aureus سميناه selective صح؟ برضه differential لأنه + +605 +00:43:30,070 --> 00:43:34,350 +للأسف خلينا selective للـ Staphylococci بس مش للـ + +606 +00:43:34,350 --> 00:43:37,610 +Staphylococcus aureus فعشان نعدل Staphylococcus + +607 +00:43:37,610 --> 00:43:41,370 +aureus خلينا differential Staphylococcus aureus + +608 +00:43:41,370 --> 00:43:46,290 +بتبين صفراء وما قعداها بتبين حمراء أحمر فصار selective + +609 +00:43:46,290 --> 00:43:51,850 +and أعتقد أن هناك الصورة بتكون واضحة تماما أن أنا + +610 +00:43:51,850 --> 00:43:55,410 +أستطيع أن أصمم أوساط زراعية في الـ microbiology و + +611 +00:43:55,410 --> 00:43:59,650 +أنا بأقولك كده مرة هذا التصميم يجب أن لا يقتصر على + +612 +00:43:59,650 --> 00:44:03,170 +من صممه كمان أنتم عندكوا المقدرة على التفكير و + +613 +00:44:03,170 --> 00:44:06,070 +عندكوا المقدرة على أنكوا لو طلبت منا في يوم من + +614 +00:44:06,070 --> 00:44:09,870 +الأيام أن نعمل modification أو ممكن نفكر احنا في + +615 +00:44:09,870 --> 00:44:15,120 +modification أو نخترع أوساط جديدة في مقدرتنا و بس + +616 +00:44:15,120 --> 00:44:19,560 +بدنا نقضي وقت أطول في دراسة المعلومات المتعلقة + +617 +00:44:19,560 --> 00:44:23,780 +ببعض الـ micro organism حتى نستطيع نوفر لها الصفات + +618 +00:44:23,780 --> 00:44:26,140 +المناسبة نكمل المرة الجاية إن شاء الله كل العالم و + +619 +00:44:26,140 --> 00:44:26,800 +أنتم بخير diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/T0ZBN7Mv6dQ_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/T0ZBN7Mv6dQ_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..7500997c29f474eb10017307ac1f70c2b574769e --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/T0ZBN7Mv6dQ_postprocess.srt @@ -0,0 +1,2476 @@ +1 +00:00:21,700 --> 00:00:24,280 +بسم الله الرحمن الرحيم نكمل ما بدأناه في ال + +2 +00:00:24,280 --> 00:00:28,660 +bacterial physiology المحاضرة السابقة تحدثنا عن + +3 +00:00:28,660 --> 00:00:32,380 +بعض العوامل بتأثر على نمو البكتيريا من nutrient و + +4 +00:00:32,380 --> 00:00:36,640 +من pH و من حرارة and osmotic pressure الاخرى و في + +5 +00:00:36,640 --> 00:00:39,820 +الآخر المحاضرة اتطرقنا لل doubling time او زمان + +6 +00:00:39,820 --> 00:00:43,280 +الانقسام و simple binary equation اليوم هتطلع لقصة + +7 +00:00:43,280 --> 00:00:47,900 +حياة البكتيريا كلنا كبشر إن قصص مختلفة و متنوعة + +8 +00:00:47,900 --> 00:00:55,020 +لكن تنتهي نهاية واحدة بالموتقصص غير هيك البكتيريا + +9 +00:00:55,020 --> 00:00:59,580 +القصة اللى مالكه على الصبورة الآن هي القصة + +10 +00:00:59,580 --> 00:01:05,480 +المشتركة لكل أنواع البكتيريا عندما تقع البكتيريا + +11 +00:01:05,480 --> 00:01:11,060 +في غذاء او في اي ديقة فيها مواد غذائية لا تبدأ + +12 +00:01:11,060 --> 00:01:16,160 +بالانقسام مباشرة وانما تدرس + +13 +00:01:16,160 --> 00:01:22,330 +مكونات الوسطوبالتالي تحتاج إلى فترة تعمل + +14 +00:01:22,330 --> 00:01:25,650 +adaptation بتجهز ال enzyme system تبعها واحنا + +15 +00:01:25,650 --> 00:01:28,550 +حكينالكم في السابق قولنالكم مثلا لو كان في جليكوز + +16 +00:01:28,550 --> 00:01:33,090 +ولاكتوز وكان فينا starch ويقادر على تناول هذه + +17 +00:01:33,090 --> 00:01:38,070 +العطر مثلها ده ستقوم بتجهيز ال enzyme system بحيث + +18 +00:01:38,070 --> 00:01:41,390 +انه تقول انه أنول أوفر للطاقة وفي دل الحالة هو + +19 +00:01:41,390 --> 00:01:45,340 +مين؟ الجليكوزعند هذا النقطة لازم نجرب و نتكلم + +20 +00:01:45,340 --> 00:01:49,380 +مصطلحين مهمين جدا مش مطلوبين على السبورة ال enzyme + +21 +00:01:49,380 --> 00:01:53,460 +ممكن نقسمهم احنا الى constitutive enzymes و كلمة + +22 +00:01:53,460 --> 00:01:56,320 +constitutive enzymes يعني enzymes that are + +23 +00:01:56,320 --> 00:02:02,760 +produced as long as the cell is growing او living + +24 +00:02:02,760 --> 00:02:08,120 +يعني بمعنى اخر ان الانزيمات هتنتج طالما الخلية حية + +25 +00:02:08,120 --> 00:02:11,740 +يعني مافيش عليها control مافيش عليها control بمعنى + +26 +00:02:11,740 --> 00:02:17,940 +اخر دايما موجودةبينما الـ induced enzymes they + +27 +00:02:17,940 --> 00:02:20,420 +will not be produced unless their substrate is + +28 +00:02:20,420 --> 00:02:24,500 +there يعني لما يصير فيه substrate لهم، فيه داعي، + +29 +00:02:24,500 --> 00:02:28,660 +فيه برتقال، بنجيب سكين من المطبخ مافيش برتقال، + +30 +00:02:28,660 --> 00:02:32,040 +يجيب خل السكين في المطبخ وصي الصورة، هذا اسمه اسمه + +31 +00:02:32,040 --> 00:02:38,100 +induced enzyme مين الأوفر الطاقة قلناها؟ ال + +32 +00:02:38,100 --> 00:02:41,260 +constitutive لأنه موجود، it is already there يعني + +33 +00:02:41,260 --> 00:02:47,520 +سواء شئ ما بينا، هو موجودوبتبدأ البكتيريا بالتغذية + +34 +00:02:47,520 --> 00:02:54,020 +لكن تنمو وتزداد حجما اللي قدامي في ال quick curve + +35 +00:02:54,020 --> 00:02:56,840 +هنا على الصبورة اللي بتلاحظه بالخط الأحمر اللي هو + +36 +00:02:56,840 --> 00:03:01,880 +ال lag phase من zero time إلى time معين ماكان فيه + +37 +00:03:01,880 --> 00:03:04,540 +زيادة في عدد البكتيريا ربما يكون فيه زيادة في + +38 +00:03:04,540 --> 00:03:11,560 +الحجم لكن مافيه زيادة في العددبعد ذلك بدأت مرحلة + +39 +00:03:11,560 --> 00:03:15,740 +أخرى من الانقسام بالخط الأزرق أو اللول الأزرق صار + +40 +00:03:15,740 --> 00:03:22,760 +الانقسام 2 4 8 16 32 إيش بنسمي هذا؟ بنسميه زيادة + +41 +00:03:22,760 --> 00:03:27,300 +لغاريثمية أو زيادة قصية إيه exponential أو + +42 +00:03:27,300 --> 00:03:31,460 +logarithmic increase in number وبالتالي تتضاعف في + +43 +00:03:31,460 --> 00:03:36,800 +عداد اللي هو ال bacteria الان إلى حد معينالبكتيريا + +44 +00:03:36,800 --> 00:03:41,400 +طبعا المقول ده في اناء مغلق، الاناء المغلق في كمية + +45 +00:03:41,400 --> 00:03:45,520 +محدودة من مين؟ من ال nutrients وبالتالي ال + +46 +00:03:45,520 --> 00:03:49,700 +nutrient بتبدأ بالنفاذ هذا واحد، رقم اتنين ال pH + +47 +00:03:49,700 --> 00:03:52,120 +تبقى ال media as a result of the metabolic + +48 +00:03:52,120 --> 00:03:56,540 +processes of the bacteria will decrease، بنزل ال + +49 +00:03:56,540 --> 00:04:00,880 +pH ويصير acidic ال accumulation of waste products + +50 +00:04:00,880 --> 00:04:06,430 +بتزيد، اللي هو تراكم المخلفات تبع نمو البكتيرياال + +51 +00:04:06,430 --> 00:04:09,830 +Oxygen إذا كان البكتيريا بهذه ال Oxygen قد يقل في + +52 +00:04:09,830 --> 00:04:14,930 +الوسط لأنك عندي مجموعة من العناصر بدأت إيه؟ خلّيني + +53 +00:04:14,930 --> 00:04:21,110 +أقول بطرة متالية للبكتيريا فصار عندي بعض البكتيريا + +54 +00:04:21,110 --> 00:04:25,230 +بتموت و البعض الأخر بينقسم و لو كنا في أي مجتمع في + +55 +00:04:25,230 --> 00:04:29,490 +الدنيا عدله فيها يساوي عد المواليد لن يكون هناك + +56 +00:04:29,490 --> 00:04:34,090 +زيادة في العدد انظروا إلى الخط الأخضرالخط الأكبر + +57 +00:04:34,090 --> 00:04:38,590 +اللي ماناها دلوقتي بالمقابل في time فترة زمنية + +58 +00:04:38,590 --> 00:04:42,890 +الفترة الزمنية هذه بتقول إنه لا يدد زيادة في العدد + +59 +00:04:42,890 --> 00:04:47,150 +لحد معينة مثلا منها مرحلة الثبات أو stationary + +60 +00:04:47,150 --> 00:04:53,330 +phase كل ما ذكرناه من ظروف قلنا ساقت فأدت إلى ال + +61 +00:04:53,330 --> 00:04:59,390 +stationary phase عندما تزداد سوء بيصير عدله فياه + +62 +00:04:59,390 --> 00:05:04,540 +أكبر من عدل الانقساماتو بالظبط و الآن بيبدأ العلبة + +63 +00:05:04,540 --> 00:05:08,120 +أو ال number بتباعها to decline او بنسمي decline + +64 +00:05:08,120 --> 00:05:11,960 +phase او بنسمي death phase اللي هو اللون الزهري + +65 +00:05:11,960 --> 00:05:15,820 +هذا اللي موجود أمامك و إذا فينا أربع مراحل بنرجعهم + +66 +00:05:15,820 --> 00:05:19,160 +مع بعض على السطورة اللي هم ال lag phase وهو لا + +67 +00:05:19,160 --> 00:05:21,760 +يولد فيه increase انما يولد فيه مرحلة تحضير و + +68 +00:05:21,760 --> 00:05:25,280 +adaptation ال logarithmic phase وهو المرحلة الأكبر + +69 +00:05:25,280 --> 00:05:28,840 +في مرحلة النمو ال stationary مرحلة ثبات و مرحلة + +70 +00:05:28,840 --> 00:05:33,300 +أخيرة اللي هي decline او death phaseقصة حياة أي + +71 +00:05:33,300 --> 00:05:37,920 +بكتيريا غالبا لا تتعدى هذه الخطوط الفترة الزمنية + +72 +00:05:37,920 --> 00:05:42,180 +قد تطول قد تقصر لكن في الآخر الشكل اللي قدامنا و + +73 +00:05:42,180 --> 00:05:44,820 +المراحل اللي قدامنا هي اللي هتسود في هذه العملية + +74 +00:05:44,820 --> 00:05:50,040 +وسط الصورة أجر ده تبقوا معايا أهلا لنقطة كتير مهمة + +75 +00:05:50,040 --> 00:05:55,460 +بدنا نستفيد استفادة طبية فاكرين ال gram stain؟ كنا + +76 +00:05:55,460 --> 00:05:59,100 +نقول دي gram stain gram بسطف و gram negative الفرق + +77 +00:05:59,100 --> 00:06:02,650 +بينهم مبني على ال cell wallالأقل لما بيقول + +78 +00:06:02,650 --> 00:06:06,850 +البكتيريا بتموت أو في وضع سيء، بقى لكوا ال cell + +79 +00:06:06,850 --> 00:06:10,330 +wall هو الجدولة الأسمنتي، بده يقعد ثانوية لما + +80 +00:06:10,330 --> 00:06:14,270 +يتحلق؟ ولا يتحلق على طول؟لذلك لو حوالنا نصبغ + +81 +00:06:14,270 --> 00:06:19,110 +بكتيريا بال gram stain وهي تموت، وهي تتحلل، لو + +82 +00:06:19,110 --> 00:06:21,930 +كانت ال gram positive هتبين ال gram negative لإن + +83 +00:06:21,930 --> 00:06:27,870 +السلوك تبعها بدأ يتحلل، إذا بنفهم أن أفضل منطقة و + +84 +00:06:27,870 --> 00:06:32,270 +أفضل جزئي نعمل فيها gram stain هي ال logarithmic + +85 +00:06:32,270 --> 00:06:34,850 +phase اللي هي معلنة باللون الأزرق على اللوحة اللي + +86 +00:06:34,850 --> 00:06:40,780 +هي أمامكليش؟ عشان كمان مرة البكتيريا بتكون في أوج + +87 +00:06:40,780 --> 00:06:43,820 +انقسامها أفضل ما يكون ال cell wall بيكون في هذه + +88 +00:06:43,820 --> 00:06:47,300 +المنطقة طبعا في ال industrial microbiology أو في + +89 +00:06:47,300 --> 00:06:49,960 +ال microbiology الصينية قد يكون ده كلام صحيح يعني + +90 +00:06:49,960 --> 00:06:53,280 +مش هذه الفترة هي المفضلة لدينا ممكن تكون فترات + +91 +00:06:53,280 --> 00:06:57,320 +أخرى مفضلة لكن احنا معنيين بهذه الفترة + +92 +00:07:00,890 --> 00:07:03,010 +صار عندنا فكرة عن ال growth نتكلم شوية عن ال + +93 +00:07:03,010 --> 00:07:05,830 +metabolism و ال metabolism احنا كده مرة هنا + +94 +00:07:05,830 --> 00:07:09,230 +مابنناقش biochemistry مابننرجع كل ال biochemistry + +95 +00:07:09,230 --> 00:07:14,010 +و انما بدنا نستغل بعض المعلومات في ال metabolism + +96 +00:07:14,010 --> 00:07:18,570 +حتى نستطيع من خلالها نتعرف على البكتيري يعني ال + +97 +00:07:18,570 --> 00:07:21,910 +metabolism هنا بغرض مين؟ التعرف على البكتيري + +98 +00:07:21,910 --> 00:07:26,320 +energy metabolismهتكلم عنه شوية هتكلم عن ال + +99 +00:07:26,320 --> 00:07:28,840 +medical important metabolic processes بمعنى + +100 +00:07:28,840 --> 00:07:32,940 +العمليات اللي لها علاقة بال medicine بال medical + +101 +00:07:32,940 --> 00:07:36,180 +microbiology تخيلوا بكتيريا as a waste product + +102 +00:07:36,180 --> 00:07:42,140 +تمتش تكسين أكيد التكسين هذا هيأثب عليه تمام؟ و زي + +103 +00:07:42,140 --> 00:07:45,080 +أي metabolism في الدنيا إما anabolic process أو + +104 +00:07:45,080 --> 00:07:49,960 +catabolic process إما عمليات بناء أو عملياتتعالوا + +105 +00:07:49,960 --> 00:07:53,720 +نقعد هذه اللوحة اللي أمامكوا اللوحة كتير جدا مهمة + +106 +00:07:53,720 --> 00:07:58,440 +لنا و اليوم يجب أن نؤكد على الفروقات الثلاث بين + +107 +00:07:58,440 --> 00:08:01,780 +الثلاث عناصر اللي موجودة أمامنا aerobic + +108 +00:08:01,780 --> 00:08:04,520 +respiration, anaerobic respiration and + +109 +00:08:04,520 --> 00:08:09,980 +fermentation التلاتة هي عمليات قد تقوم بها + +110 +00:08:09,980 --> 00:08:14,840 +البكتيريا وبالتالي بنميز بينهم التلاتة الأولى هي + +111 +00:08:14,840 --> 00:08:17,860 +الوحيدة اللي بتستهلك ال oxygen اللي هي aerobic + +112 +00:08:17,860 --> 00:08:23,250 +respirationبينما ال anaerobic respiration و ال + +113 +00:08:23,250 --> 00:08:27,150 +fermentation بتموا في غياب ال oxygen، طب كيف بنا + +114 +00:08:27,150 --> 00:08:30,810 +نميز بينهم من ناحية biochemistry؟ الأولى اللي هي + +115 +00:08:30,810 --> 00:08:34,710 +ال aerobic respiration، ال oxygen is the final + +116 +00:08:34,710 --> 00:08:38,190 +electron acceptor، + +117 +00:08:38,190 --> 00:08:39,110 +أظن واضحة النقطة هذه + +118 +00:08:43,420 --> 00:08:48,340 +استفادة من الكربوهيدرات بتتم في وجود الأكسجين one + +119 +00:08:48,340 --> 00:08:54,260 +molecule will produce 36 ATPs نيجي الآن لل + +120 +00:08:54,260 --> 00:08:57,260 +Anaerobic Respiration و ال fermentation كيبنا نميز + +121 +00:08:57,260 --> 00:09:00,560 +بينهم و خاصة إنه في ورية شبه إنه بتتموا في غياب + +122 +00:09:00,560 --> 00:09:04,720 +الأكسجين ال Anaerobic Respiration ال final + +123 +00:09:04,720 --> 00:09:08,380 +electron acceptor بيكون inorganic compound مركّب + +124 +00:09:08,380 --> 00:09:12,960 +غير عضوي غير الأكسجينInorganic compound غير ال + +125 +00:09:12,960 --> 00:09:16,900 +Oxygen بينما ال fermentation بيكون ال final + +126 +00:09:16,900 --> 00:09:20,960 +electron acceptor هو organic compound وصلت الصورة + +127 +00:09:20,960 --> 00:09:25,740 +Oxygen للأولى، Inorganic compound للتانية، تلاتة، + +128 +00:09:25,740 --> 00:09:29,620 +أكتر واحد more efficient أو the most efficient + +129 +00:09:29,620 --> 00:09:32,300 +اللي هي aerobic respiration، the least efficient + +130 +00:09:32,300 --> 00:09:38,710 +اللي هي ال fermentation هي الأقل انتجة للطاقةكيف + +131 +00:09:38,710 --> 00:09:41,650 +نستغل وهذه اللوحة اللي أمامنا وكتير مهمة نضلنا + +132 +00:09:41,650 --> 00:09:46,110 +مركزين عليها طوال الوقت حتى نستطيع أن نفهم ماذا + +133 +00:09:46,110 --> 00:09:51,890 +يعني بهذه اللوحة احنا في ال micro biology قد يظن + +134 +00:09:51,890 --> 00:09:54,710 +البعض انه كون الميكروبات دقيقة و احنا ممكن + +135 +00:09:54,710 --> 00:09:59,010 +المحاضرة مش المواد اللي جابلها تحدثنا عن استخدامات + +136 +00:09:59,010 --> 00:10:02,550 +الميكروسكوبات يمكن احنا اقل الناس اللي بنستخدم في + +137 +00:10:02,550 --> 00:10:07,620 +مجلة الميكروسكوبليش؟ طورنا أنظمة .. أنظمة الـ + +138 +00:10:07,620 --> 00:10:10,800 +detection بالتغيير في الألوان، بنوعها على مين؟ + +139 +00:10:10,800 --> 00:10:13,320 +بنوعها تغيروا في الـ pH فمثلا الصورة اللي قدامكوا + +140 +00:10:13,320 --> 00:10:17,620 +هذه اللون الأحمر هو اللون الأصلي للوسط زُنع + +141 +00:10:17,620 --> 00:10:21,500 +ببكتيريا فتحول إلى لون الأصفر، ليش تحول إلى لون + +142 +00:10:21,500 --> 00:10:25,560 +الأصفر؟ لأن البكتيريا عملت fermentation أو عملت + +143 +00:10:25,560 --> 00:10:30,460 +metabolism فخفضت ال pH واحنا حاطين pH indicator، + +144 +00:10:30,460 --> 00:10:34,450 +تمام؟فإيش يعني pH Indicator؟ يعني مُركّب يتغير + +145 +00:10:34,450 --> 00:10:38,930 +لونه بتغير ال pH فتغير من اللون الأحمر لللون + +146 +00:10:38,930 --> 00:10:41,590 +الأصفر إعرفنا إنه العينة أو ال .. أو ال .. + +147 +00:10:41,590 --> 00:10:45,830 +البكتيريا positive إيش يعني positive؟ يعني استطالت + +148 +00:10:45,830 --> 00:10:49,690 +أن تقوم بالمهمة إيش المهمة؟ to ferment أو to + +149 +00:10:49,690 --> 00:10:52,530 +metabolize على سبيل المثال ال dextrose أو ال + +150 +00:10:52,530 --> 00:10:56,970 +glucose طيب التنتين صفر صح؟ اللي في الصورة التنتين + +151 +00:10:56,970 --> 00:11:00,630 +صفر في فرق بينهم؟ في واحدة مطالعة غاز، شايفينها؟ + +152 +00:11:04,720 --> 00:11:08,160 +انطلعها غازي بعد الحكي انه في كمان شغل عملناها + +153 +00:11:08,160 --> 00:11:11,140 +detection في ال metabolism انه صار fermentation + +154 +00:11:11,140 --> 00:11:15,640 +positive or negative كيف عرفناها؟ بتغيير اللون في + +155 +00:11:15,640 --> 00:11:18,360 +كمان شغل تاني ممكن نعرفها انه fermentation with + +156 +00:11:18,360 --> 00:11:22,280 +gas ولا fermentation with no gas هاي كمان صفة + +157 +00:11:22,280 --> 00:11:26,160 +جديدة يعني بدل الفحص سرنانيا محصانة بدل الصفة + +158 +00:11:26,160 --> 00:11:31,250 +سرناندي صفة تانيةمثال آخر على الـ metabolic + +159 +00:11:31,250 --> 00:11:36,150 +process اللى بتساعدنا في عملية اللى هو التعرف على + +160 +00:11:36,150 --> 00:11:41,930 +البكتيريا فحص اسمه methyl red ال + +161 +00:11:41,930 --> 00:11:46,810 +methyl red هو عبارة عن برضه pH indicatorوسمى الفحص + +162 +00:11:46,810 --> 00:11:51,310 +باسم الـ PH Indicator المستخدم نظرا ليش؟ لأنه + +163 +00:11:51,310 --> 00:11:55,510 +بنضيف الـ Methyl Red مش مباشرة مع الميديا وإنما + +164 +00:11:55,510 --> 00:11:58,690 +بعد نمو البكتيريا يعني بمعنى أخر نزرع البكتيريا في + +165 +00:11:58,690 --> 00:12:04,030 +المبوبة تمام؟ فيها جلوكوز و بعد مرور 24 ساعة بنضيف + +166 +00:12:04,030 --> 00:12:09,730 +عليها الـ Methyl Red إذا تحول الوسط إلى اللون + +167 +00:12:09,730 --> 00:12:13,720 +الأحمر كما نرى في هذه الصورةده بيكون poster ماما + +168 +00:12:13,720 --> 00:12:19,460 +اللون الأحمر انه ال pH تبع ال media وصل لأقل من + +169 +00:12:19,460 --> 00:12:23,800 +أربعة و هم هتدرسوه بالتفصيل في المعمل ان شاء الله + +170 +00:12:23,800 --> 00:12:28,060 +و هتشتغلوا شغل عملي بإذن الله لكن في الآخر ميازت + +171 +00:12:28,060 --> 00:12:31,700 +بين بكتيريا methyl red poster و أخرى methyl red + +172 +00:12:31,700 --> 00:12:35,280 +ash negative اللون الأحمر poster و اللون الأصفر + +173 +00:12:35,280 --> 00:12:35,740 +negative + +174 +00:12:38,500 --> 00:12:42,080 +الـ Bogus Proscour هو نوع آخر من الفحوصات المعتمدة + +175 +00:12:42,080 --> 00:12:45,380 +على الـ metabolic processes هناك بعض البكتيريا + +176 +00:12:45,380 --> 00:12:51,880 +عندها metabolic pathway اسمه 2,3-butylene glycol + +177 +00:12:51,880 --> 00:12:59,960 +أحد ال precursors المميزة مركب اسمه أسيتوين وليس + +178 +00:12:59,960 --> 00:13:04,220 +أسيتون في فرق أسيتوين هو اسم تاني + +179 +00:13:04,220 --> 00:13:07,520 +أسيتالميثالكاربينول برضه هدرسه في المعمل إن شاء + +180 +00:13:07,520 --> 00:13:12,790 +اللههذا المُركّب إن موجد فبمؤشر على وجود الـ 2,3 + +181 +00:13:12,790 --> 00:13:17,110 +-butanyl glycol fermentation pathway إذا لم يوجد + +182 +00:13:17,110 --> 00:13:20,850 +فمعناه الحاجة أن البكتيريا لا تمتلك هذا الpathway + +183 +00:13:20,850 --> 00:13:25,650 +بعد ما بننمي البكتيريا بنضيف two compounds يعني + +184 +00:13:25,650 --> 00:13:29,990 +two detectors إذا اتحول الأنبوبة إلى لون الأحمر + +185 +00:13:29,990 --> 00:13:35,410 +بصي فيما اتحول إلى لون الأحمر نيجاتي إستعت من خلال + +186 +00:13:35,410 --> 00:13:36,330 +فحص + +187 +00:13:39,410 --> 00:13:43,050 +محرف على وجود metabolic pathway، وانا متكلم عن مين + +188 +00:13:43,050 --> 00:13:46,790 +احنا الآن في المحاضرة هاي؟ متكلم عن ال bacterial + +189 +00:13:46,790 --> 00:13:51,830 +physiology، عن ال growth and metabolismفاستغلنا ال + +190 +00:13:51,830 --> 00:13:55,570 +Metabolism في التفريق بين أنواع ال micro .. ال + +191 +00:13:55,570 --> 00:13:58,270 +micro organism و أول محاضرة .. إذا بترجعوا + +192 +00:13:58,270 --> 00:14:01,530 +المحاضرة الأولى هتتذكروا إن أنا قلتلكوا معلومة جدا + +193 +00:14:01,530 --> 00:14:04,490 +إن ما يميز ال micro organism هو ال metabolic + +194 +00:14:04,490 --> 00:14:08,970 +biodiversity التنوع في ال metabolism بكتيريا تتغذى + +195 +00:14:08,970 --> 00:14:12,450 +على الجلوكوز أخرى على الجلوكوز زاد ال lactose أخرى + +196 +00:14:12,450 --> 00:14:17,310 +على الجلوكوز زاد ال maltose و هكذا أنواع مختلفة من + +197 +00:14:17,310 --> 00:14:20,730 +ال substrateتستغل من قبل أنواع مختلفة من ال micro + +198 +00:14:20,730 --> 00:14:26,630 +organism حصل رابعا والصورة اللي أمامكوا واضحة كتير + +199 +00:14:26,630 --> 00:14:31,670 +أنه في لونين أزرق وأخضر ال original color للأنبوبة + +200 +00:14:31,670 --> 00:14:34,510 +هذي هو اللون الأخضر ال original color هو اللون + +201 +00:14:34,510 --> 00:14:38,830 +الأخضر إذا اتحول إلى اللون الأزرق فالفحص positive + +202 +00:14:38,830 --> 00:14:43,140 +هقولكوا كيف بتحولهذا الفحص معتبد على قدرة + +203 +00:14:43,140 --> 00:14:47,840 +البكتيريا عليها تستغل ال sodium citrate كمصدر + +204 +00:14:47,840 --> 00:14:51,520 +كربوني وحيد كمصدر كربوني وحيد مش كل البكتيريا + +205 +00:14:51,520 --> 00:14:55,100 +عندها المقدرة هذه أنواع قليلة من الميكروبات عندها + +206 +00:14:55,100 --> 00:14:58,900 +المقدرة هذه إذا بقدر أميز هذه الأنواع عن بعض + +207 +00:14:58,900 --> 00:15:02,580 +الأنواع الأخرى اللي ماعندهاش هذه المقدرة بحط + +208 +00:15:02,580 --> 00:15:06,560 +انبوبة فيها sodium citrate و فيها pH indicator + +209 +00:15:06,560 --> 00:15:12,140 +اسمه bromthamol blueإذا البكتيريا استهلكت .. + +210 +00:15:12,140 --> 00:15:17,060 +استهلكت .. ال .. ال sodium citrate هتستهلك معاه + +211 +00:15:17,060 --> 00:15:22,800 +مركب عضوي آخر بيرفع ال pH إلى أعلى، أعلى يعني + +212 +00:15:22,800 --> 00:15:30,170 +alkaline، لما يرتفع ال pH إلى alkalineبيتحول اللون + +213 +00:15:30,170 --> 00:15:32,790 +الاسمه bromethymol الـ pH Indicator اللي اسمه + +214 +00:15:32,790 --> 00:15:36,810 +bromethymol blue إلى من أخضر إلى أسرع وابتدا اللي + +215 +00:15:36,810 --> 00:15:40,310 +بيقول الفحص هي positive إذا بقى كما هو الفحص + +216 +00:15:40,310 --> 00:15:44,430 +negative هذا كان فحص اللي عارفينه الآن ال + +217 +00:15:44,430 --> 00:15:46,570 +carbohydrate fermentation test خدوا بالكم مش فحص + +218 +00:15:46,570 --> 00:15:51,980 +واحد ال carbohydrate fermentation testمش بس لأ مش + +219 +00:15:51,980 --> 00:15:56,500 +بس كمان هيك، لكل carbohydrate بقدر أعمل فحص، يعني + +220 +00:15:56,500 --> 00:15:59,820 +بعمل للجليكوز، لللاكتوز، للستاكروز، للمريتول، + +221 +00:15:59,820 --> 00:16:04,460 +للسوربيتول، لمجموعة كبيرة من السكريات، وكلها بقدر + +222 +00:16:04,460 --> 00:16:08,240 +أخد منها الصفتين، ال post fermentation زاد اللي هو + +223 +00:16:08,240 --> 00:16:13,710 +ال gas productionالثلاث بحثات التانية كانوا methyl + +224 +00:16:13,710 --> 00:16:17,910 +red, vox proscarin و citrate هنتقل إلى الرابع في + +225 +00:16:17,910 --> 00:16:21,230 +السلسلة اللي هم مجموعين مع بعض اللي اسمه Endolpist + +226 +00:16:29,830 --> 00:16:35,290 +الان فحص الاندول هو وسيلة للكشف عن مقدرة البكتيريا + +227 +00:16:35,290 --> 00:16:40,070 +على إنتاج إنزيم اسمه triptophanase، بيكسر مين هذا؟ + +228 +00:16:40,070 --> 00:16:43,790 +triptophan، تكسير لـ triptophan بينتج عنه مركب + +229 +00:16:43,790 --> 00:16:47,950 +اسمه اندولهذا لأن دول بتتم الكشف عنه باضافة برضه + +230 +00:16:47,950 --> 00:16:50,670 +بعض المُركّبات الكيميائية باسمها Kovacs reagents + +231 +00:16:50,670 --> 00:16:56,550 +إذا تكون ال red range حلقة الحمراء هذه إذا تكونت + +232 +00:16:56,550 --> 00:17:01,890 +فالفحص positive يبقى قدامي هنابصتف لأنه فيه red + +233 +00:17:01,890 --> 00:17:05,470 +ring ال red ring نتيجة على أنه المادة اللي بنضيفها + +234 +00:17:05,470 --> 00:17:10,630 +فيها كحول بتستخلص ال indol و المادة الأخرى الملونة + +235 +00:17:10,630 --> 00:17:14,550 +وبالتالي بتستخلصه و بترفعه وان على السطح فبتكون + +236 +00:17:14,550 --> 00:17:17,570 +red ring على السطح في ال negative إيش فيها نهاية + +237 +00:17:17,570 --> 00:17:23,050 +في ال negative؟ زي ما شايفين مافيش لون لون ال + +238 +00:17:23,050 --> 00:17:26,270 +reagent فقط اللي هتنضف فيهاالأنبوبة الأولى اللى + +239 +00:17:26,270 --> 00:17:30,430 +على الجهة هاى هي أنبوبة غير مزروعة أساسا للمقارنة + +240 +00:17:30,430 --> 00:17:33,190 +عشان نقدر نشوف انه صار نمو ولا ماصارش نمو في + +241 +00:17:33,190 --> 00:17:36,470 +الأنبوبة الأخرى انا كان فحص صاروا الأن اربعة غير + +242 +00:17:36,470 --> 00:17:39,410 +الأولة تبقوا من الأولة نهرج بيكربوا ديه صار عندي + +243 +00:17:39,410 --> 00:17:43,770 +اندول ميتيل ريد بوكوس بروسكوار وسايترين انا + +244 +00:17:43,770 --> 00:17:46,750 +الترتيب اللى في اللوحة مش هو الترتيب اللى انا + +245 +00:17:46,750 --> 00:17:49,950 +بدهيه الترتيب اللى انا ذاكرته كمرة اندول + +246 +00:17:56,130 --> 00:17:59,130 +بشكل سريع يا بنات بنرجع للأروحات الأربع اللي + +247 +00:17:59,130 --> 00:18:03,030 +شفناهم ونطلع كلها تحت صورة إيش مكتوبة أو الصورة + +248 +00:18:03,030 --> 00:18:06,370 +الأولى اللي هي إيشريشيا كولاي و إنتيروباكتر + +249 +00:18:06,370 --> 00:18:10,250 +positive and negative ال default إشريشيا كولاي ال + +250 +00:18:10,250 --> 00:18:11,730 +negative الإنتيروباكتر + +251 +00:18:26,040 --> 00:18:30,980 +يبجى انا عندى اربع فحاصات قارنت بين two organisms, + +252 +00:18:31,200 --> 00:18:34,560 +two similar organisms وقت انتنى طلعوا positive + +253 +00:18:34,560 --> 00:18:39,440 +لفحص الشريشة كلها طلعت positive لان دول ميذرد و + +254 +00:18:39,440 --> 00:18:42,320 +negative للتانين للتانين الباجين و ال enterovector + +255 +00:18:42,320 --> 00:18:45,660 +negative للأولانين التانين و positive للتانين + +256 +00:18:45,660 --> 00:18:50,780 +التانينطيب ألم يكن يكفينا فحص واحد، انقول سيترات + +257 +00:18:50,780 --> 00:18:54,980 +بسط و سيترات نيجاتيف؟ خد القاعدة اللى لازم احنا + +258 +00:18:54,980 --> 00:18:58,220 +يعني نكتبها بحروف من ده في ال diagnostic + +259 +00:18:58,220 --> 00:19:01,560 +microbiology او في ال medical the more test you + +260 +00:19:01,560 --> 00:19:05,040 +use the more accurate is your diagnosis يعني كل ما + +261 +00:19:05,040 --> 00:19:09,720 +زاد عال الفحصات اللى بمستخدمهم كل ما زاد دقة عملية + +262 +00:19:09,720 --> 00:19:10,900 +اللى هو التعريف + +263 +00:19:14,280 --> 00:19:16,340 +الـ differentiation فاكرينه لما كنت أقولكوا + +264 +00:19:16,340 --> 00:19:20,300 +سالمونيلا وشيجيلا و مميز منهم بال motility غالبا + +265 +00:19:20,300 --> 00:19:25,800 +نعم هو فحص مميز لكن لا نعتمد عليه لوحده كفحص واحد + +266 +00:19:25,800 --> 00:19:31,160 +و لا نشبه معاه فحصات أخرى نرجع و نكمل ال .. ال .. + +267 +00:19:31,160 --> 00:19:36,400 +ال MVIC ال MVIC اللي قدامكوا هو رموز لأربع فحصات + +268 +00:19:36,400 --> 00:19:41,670 +ال I in doll و ال M مثيل Red و ال V أو ال VPبوجرس + +269 +00:19:41,670 --> 00:19:45,270 +بروس كوار و ال C سيتيطر على ال I just to make it + +270 +00:19:45,270 --> 00:19:49,730 +قابل للنطق ان نقول Invict تمام؟ وشايفين في الجدول + +271 +00:19:49,730 --> 00:19:55,010 +التحت ان الشريشة كولاي positive positive negative + +272 +00:19:55,010 --> 00:19:58,390 +negative بينما ال enterobacter negative negative + +273 +00:19:58,390 --> 00:20:02,990 +positive positive اعتقد ان هذه المجموعة من + +274 +00:20:02,990 --> 00:20:07,310 +الفحوصات أثبتت لنا beyond reasonable doubt يعني + +275 +00:20:07,310 --> 00:20:11,980 +بدون أدنى شكإنه ال metabolic processes ال + +276 +00:20:11,980 --> 00:20:17,340 +biochemistry تبعة البكتيريا مهمة جدا في عملية + +277 +00:20:17,340 --> 00:20:22,060 +تمييز البكتيريا أعطيكوا توضيح كامل من ألفه إلى ياه + +278 +00:20:22,060 --> 00:20:27,580 +تكرر ال grams تان؟ قلنا جدش عظيم ال grams تان؟ + +279 +00:20:27,580 --> 00:20:30,640 +بشكل كبير قلنا عنه عظيم قلنا عنه إنه بميز بين + +280 +00:20:30,640 --> 00:20:34,160 +مجموعتين كبار gram بسط و gram negative روحنا لل + +281 +00:20:34,160 --> 00:20:36,460 +gram negative احنا اللي قاعدين نتكلم عن بكتيريا + +282 +00:20:36,460 --> 00:20:39,340 +مجهولة عزلناهالما عملتنا gramستين طلعت gram + +283 +00:20:39,340 --> 00:20:45,260 +نيجاتيف طلعت شكلها لجناها بسلاي فصار عندي انا ربع + +284 +00:20:45,260 --> 00:20:51,420 +من اربع ايش هم الاربعة؟ gram positive coexi و gram + +285 +00:20:51,420 --> 00:20:54,640 +negative coexi gram positive بسلاي و gram negative + +286 +00:20:54,640 --> 00:20:59,660 +بسلاي يبقى صار عندي انا واحد من اربع ربع هذا الربع + +287 +00:20:59,660 --> 00:21:04,080 +بتبقى اجسام كمان اجسام عندي اربع اصناف مش للحفظ + +288 +00:21:04,080 --> 00:21:09,000 +الكلام اللي بحكيه لان لكن للتوضيحانتيرو بكترياسي + +289 +00:21:09,000 --> 00:21:13,340 +family كبيرة ضخمة Sodomonas Acinetobacter و + +290 +00:21:13,340 --> 00:21:17,080 +Vibrium أربع أصناف طلعت البكتيريا تبعتي تابعة + +291 +00:21:17,080 --> 00:21:21,720 +لعائلة اسمها انتيرو بكترياسي الانتيرو بكترياسي + +292 +00:21:21,720 --> 00:21:27,270 +فيها 200 اسمكلهم بيمتلكوا نفس الصفة اللى انا قلت + +293 +00:21:27,270 --> 00:21:30,910 +عشان انا مش عارف انترو بكترياسي يعني تمتلك كلهم + +294 +00:21:30,910 --> 00:21:33,850 +المتان بيمتلكوا نفس الصفة انهم يكونوا نفس العائلة + +295 +00:21:33,850 --> 00:21:37,910 +لكن جوا أسماء مختلفين بدي اميزهم عن بعض من ضمن ال + +296 +00:21:37,910 --> 00:21:42,750 +انترو بكترياسي اشيريشيا كولاي و انترو بكتر بينقسم + +297 +00:21:42,750 --> 00:21:45,610 +الانترو بكترياسي الى lactose fermenter و من ال + +298 +00:21:45,610 --> 00:21:48,950 +lactose fermenter هدولة طلعتوا ضمن مين ضمن ال + +299 +00:21:48,950 --> 00:21:52,370 +lactose fermenter شوفوا قدش اوجه التشابه بينهم + +300 +00:21:52,370 --> 00:21:59,510 +ترجعوا بالجورلكتوز فرمنتر، جليكوز فرمنتر، أكسيدايز + +301 +00:21:59,510 --> 00:22:03,430 +نيجاتيف، فاكولتاتف أناقروك، كل هذه صفات مفتركة بين + +302 +00:22:03,430 --> 00:22:09,050 +الانتروباكترا وبين الاشريشيا كولا طب أوجه تشابهم + +303 +00:22:09,050 --> 00:22:12,770 +متعددة و كثيرة يبقى في حاجة لإن هنا الميزهم عن + +304 +00:22:12,770 --> 00:22:16,890 +بعضهم من البعض، مين الميز أنهيهم؟ال biochemical + +305 +00:22:16,890 --> 00:22:20,570 +reactions لم نستطع نميزهم لأ بجرامس تاني ولا + +306 +00:22:20,570 --> 00:22:23,350 +بتقنية مختلفة أخرى ميزناهم بال biochemical + +307 +00:22:23,350 --> 00:22:26,950 +reactions لذلك تزوغ مهم جدا من ما عملكم هذا الفصل + +308 +00:22:26,950 --> 00:22:32,330 +هيكون مهتم بإجراءها بفهم و إجراء هذه الفحوصات اللي + +309 +00:22:32,330 --> 00:22:36,490 +هنستخدمها لاحقا في ال diagnostic microbial بتعرف + +310 +00:22:36,490 --> 00:22:42,520 +على ال micro organismفي كمان بعض الفحصات الأخرى + +311 +00:22:42,520 --> 00:22:45,060 +الكثيرة المتنوعة أكثر من ما أنا صادقه في هذه + +312 +00:22:45,060 --> 00:22:49,160 +المحاضرة لكن أحدها حاجة اسمها hydrogen sulfide + +313 +00:22:49,160 --> 00:22:56,700 +production H2S الـ H2S غاز عادي من اللون لكن + +314 +00:22:56,700 --> 00:23:02,590 +رائحته كريهة تعرفوها وسموها رائحت الويب الفاسداللي + +315 +00:23:02,590 --> 00:23:05,470 +بنشم بعض الأحيان في الشوارع اللي يكون فيها مجالي + +316 +00:23:05,470 --> 00:23:09,790 +راكدة هادر و لونها أسود طبعا أنا قاعد بقول عادي من + +317 +00:23:09,790 --> 00:23:15,170 +اللون هو صح؟ عادي من اللون لكن لو تفاعل مع الحديد + +318 +00:23:15,170 --> 00:23:18,990 +و الحديد موجود بكثرة في ال .. في المياه العادية، + +319 +00:23:18,990 --> 00:23:22,770 +الأشياء المصادية و الأمور هذه حديد فبيرتبط مع + +320 +00:23:22,770 --> 00:23:26,770 +الحديد بكون لون أسود، بكون لون أسود فاحنا برضه كيف + +321 +00:23:26,770 --> 00:23:30,590 +بنكشف أن البكتيرا بتنتج أو بتنتجوش؟ بنحط في ال + +322 +00:23:30,590 --> 00:23:35,780 +media حديدمركبات حديد فإذا الغاز طلع بيتحول الوسط + +323 +00:23:35,780 --> 00:23:39,740 +اللامين إلى اللامض الأزرق في فحص تاني اسمه urease + +324 +00:23:39,740 --> 00:23:44,620 +لو دخلنا دورات حمامات مش منظمة فترة طويلة بنشم + +325 +00:23:44,620 --> 00:23:46,920 +رائحة الأمونيا من بعيد رائحة كارية الأمونيا + +326 +00:23:46,920 --> 00:23:51,420 +بالنسبالنا الأمونيا هذه ناتجة على أن بكتيريا + +327 +00:23:51,420 --> 00:23:54,620 +موجودة في الدورات كسرت ال urea الموجودة في ال + +328 +00:23:54,620 --> 00:23:59,390 +urine وطلعت منها الأمونيانفس الفكرة بنقدر نستغل + +329 +00:23:59,390 --> 00:24:02,990 +اللي احنا في المعمل بنجيب قنبوبة و فيها يوريا و + +330 +00:24:02,990 --> 00:24:07,510 +بنزرع البكتيريا و إذا البكتيريا كسرت ال يوريا + +331 +00:24:07,510 --> 00:24:10,710 +هتحولها لأمون ال ammonia alkaline حطينا في الوسط + +332 +00:24:10,710 --> 00:24:15,190 +pH indicator تحول اللون إلى لون آخر و بنقول + +333 +00:24:15,190 --> 00:24:20,330 +positive أو negative في هذه الحالة إذا كل أو معظم + +334 +00:24:20,330 --> 00:24:23,930 +الفحوصات اللي مرض علينا هل شفناها تحت ال + +335 +00:24:23,930 --> 00:24:28,030 +microscope ولا طلعنا عليها تغيير ألوانتدير ألوان + +336 +00:24:28,030 --> 00:24:31,350 +دي داجة هي طبعا هتكون مفضلة بالنسبالكوا لأنه + +337 +00:24:31,350 --> 00:24:34,830 +ألوانها كتير جذابة اللي بفكر أو بالأحرى اللي في + +338 +00:24:34,830 --> 00:24:38,170 +حاجات الباقية هو ال chemical طبعا احنا كمان هتطور + +339 +00:24:38,170 --> 00:24:42,310 +ليش؟ لإنه احنا شايفين الأنابيب ما اكبرهم اللي بنحط + +340 +00:24:42,310 --> 00:24:47,270 +فيهم كبارلما نصل لمرحلة معينة نفهم ال .. ال .. ال + +341 +00:24:47,270 --> 00:24:50,310 +.. الانظمة كلها ال .. ال .. الفحصات البرجمية + +342 +00:24:50,310 --> 00:24:57,090 +هنسخطهم نزغرهم و نحط لعشرين فحص في سلسلة واحدة + +343 +00:24:57,090 --> 00:25:01,290 +مثلا ال API system هنشوف برضه نماذج مقتصرة أخرى + +344 +00:25:01,290 --> 00:25:02,470 +غير ال API system + +345 +00:25:05,080 --> 00:25:09,100 +احنا اتكلمنا على منتجات استخدمناها في التعرف على + +346 +00:25:09,100 --> 00:25:12,080 +ال microbenz تعرفنا انا اتكلم على منتجات اخرى سيئة + +347 +00:25:12,080 --> 00:25:16,660 +الذكر زي ال pyrogen ال pyrogen this is a fever + +348 +00:25:16,660 --> 00:25:21,020 +producing substance synthesized by بكتيري اش يعني + +349 +00:25:21,020 --> 00:25:25,840 +fever producing يعني لو دخل المركب ان فيريزمنا + +350 +00:25:25,840 --> 00:25:30,880 +بيصير انها حرارة مرتفعة حرارة مرتفعة خدوا بالك مش + +351 +00:25:30,880 --> 00:25:34,540 +ضرور البكتيري تكون جوه يكفي يكون ال pyrogen جوه + +352 +00:25:35,520 --> 00:25:40,160 +بتركزوا معايا في هذه المعلومة المهمة بتعرفوا ال + +353 +00:25:40,160 --> 00:25:43,660 +stringات؟ شفتوا ما هي ال stringات؟ بتكون أظن مسكرة + +354 +00:25:43,660 --> 00:25:49,580 +و معقبة، تمام؟ ال string هذه اللي تخيل إنها كانت + +355 +00:25:49,580 --> 00:25:54,320 +مغموسة ب إشريشيا كولاي و سكرناها و بعدين عقبناها + +356 +00:25:54,320 --> 00:25:57,580 +أو برنامج إشريشيا كولاي نأخد و نركب حالة نعرفها + +357 +00:25:57,580 --> 00:26:00,400 +Staphylococcus aureus تعرفولا Staphylococcus + +358 +00:26:00,400 --> 00:26:06,820 +aureus؟ لأن البكتيريا ماتت بعد ما عقبناهالكن لم + +359 +00:26:06,820 --> 00:26:10,420 +تختفي عن كوكب الأرض، لم تختفي من السرنجة، ضايق + +360 +00:26:10,420 --> 00:26:14,520 +المركبات منها، بقايا منها، زي و احنا لما .. لما + +361 +00:26:14,520 --> 00:26:19,420 +بنموت، نختفيش فجأة، بنتحلل تدريجيا ولا لا، نفس + +362 +00:26:19,420 --> 00:26:24,460 +الشيء الفكتيريا، ماتختفيش، تتحلل من ضمن المركبات + +363 +00:26:24,460 --> 00:26:27,460 +اللي بتتركها الفكتيريا، مركبات زي الـpeptidoglycan + +364 +00:26:27,460 --> 00:26:32,940 +زي الـtychoric acid، فاكرين الـtychoric acid؟ في + +365 +00:26:32,940 --> 00:26:36,420 +الـsports، dipicronic acidالـ lipovoisaccharide + +366 +00:26:36,420 --> 00:26:41,160 +مابتدمرش بسرعة أو بسهولة هذه المركبات وإن كان + +367 +00:26:41,160 --> 00:26:44,680 +البكتيريا ميتة لو استخدمنا سيرنج هذا حققنا مركب + +368 +00:26:44,680 --> 00:26:48,900 +معين مثلا antibiotic أو محلول في جسم الإنسان وداخل + +369 +00:26:48,900 --> 00:26:53,300 +جسم الإنسان حيصير عنده fever على الرغم من عدم وجود + +370 +00:26:53,300 --> 00:26:58,120 +بكتيريا وإنما مركبات اذا بعض المنتجات اللي بتعمل + +371 +00:26:58,120 --> 00:27:00,420 +fever بيسميها pyrogine + +372 +00:27:03,960 --> 00:27:07,420 +النظافة قبل التعقيم + +373 +00:27:13,950 --> 00:27:17,630 +لازم نطفو بالأول و بعدين نعقمه لإنه لابد أبدأ + +374 +00:27:17,630 --> 00:27:21,370 +ببيئة خالية أصلا من ال micro organism للأسف هذه + +375 +00:27:21,370 --> 00:27:26,190 +التقافة قد تكون غير موجودة عند المصنعين في مصالة + +376 +00:27:26,190 --> 00:27:29,650 +الأخضر، خاصة المحلية، برا لأ، برا في عندهم أنظمة + +377 +00:27:29,650 --> 00:27:33,750 +مراقبة بتخلي التقافة غصب عنهم موجودة، بس عندنا لأ، + +378 +00:27:33,750 --> 00:27:38,330 +أقولك ما لأ، هيمروا الغذاء بعملية طبخطبخ أو طهي + +379 +00:27:38,330 --> 00:27:42,470 +يعني حرارة مرتفعة يعني قطر للميكرو أورجانيزم أوك + +380 +00:27:42,470 --> 00:27:45,890 +انت قطرت للميكرو أورجانيزم لكن مركباتها ومنطلقاتها + +381 +00:27:45,890 --> 00:27:51,470 +ما زالت مولود بس هحب ندبها هنا Exotoxin و invasive + +382 +00:27:51,470 --> 00:27:58,010 +enzymes Exotoxins يعني سموم ابتنتجها البكتيريا + +383 +00:27:58,010 --> 00:28:00,450 +while alive عكس مين + +384 +00:28:08,150 --> 00:28:10,830 +الـ lipopolysaccharide اللي هو الـ lipid A اللي + +385 +00:28:10,830 --> 00:28:13,490 +موجود في الـ lipopolysaccharide في الـ outer + +386 +00:28:13,490 --> 00:28:16,250 +membrane تبع ال gram negative بكتيريا اعتقدوا ان + +387 +00:28:16,250 --> 00:28:19,470 +هذا مافيش اي خلاف كل gram negative بكتيريا تحتوي + +388 +00:28:19,470 --> 00:28:23,570 +على اندوتوكسين لكن بعض ال gram negative بكتيريا أو + +389 +00:28:23,570 --> 00:28:27,090 +بعض ال gram بصي بنتجوا ايضا اكزوتوكسين يعني + +390 +00:28:27,090 --> 00:28:30,250 +البكتيريا بتكون حية و بتنتج هذا التوكسين و اعطيكم + +391 +00:28:30,250 --> 00:28:35,280 +ميثار كتير سهل و بسيطبتعرفوا التيتانوس؟ حكينا عنه + +392 +00:28:35,280 --> 00:28:38,220 +مرة ال Clostridium Tetani أو Clostridium Tetani + +393 +00:28:38,220 --> 00:28:43,360 +لما بغز المصمار الشخص في قدمه على سبيل المثال ال + +394 +00:28:43,360 --> 00:28:47,480 +bacteria تدخل جوا ال spores و بتبدأ تتكاثر في بعض + +395 +00:28:47,480 --> 00:28:51,620 +الظروف معينة و بتنقسم في نفس المكان لا تغادر + +396 +00:28:51,620 --> 00:28:56,980 +المكان اللي دخلت منه و بتنتج ال toxin تبعها في نفس + +397 +00:28:56,980 --> 00:29:01,930 +المكانالتوكسين لأنه مُركّبات صغيرة و will diffuse + +398 +00:29:01,930 --> 00:29:05,510 +و في الآخر بيصل للدم و بروح لل central nervous + +399 +00:29:05,510 --> 00:29:09,630 +system البكتيريا الموجودة localized .. localized + +400 +00:29:09,630 --> 00:29:15,730 +يعني في مكان محدود بينما .. بينما المنتج تبعها + +401 +00:29:15,730 --> 00:29:19,350 +اللي هو ال exotoxin خرج منها و خرج من نطاق اللي + +402 +00:29:19,350 --> 00:29:21,630 +localized صار systemic و التأثير تبعه بيكون + +403 +00:29:21,630 --> 00:29:22,350 +systemic + +404 +00:29:32,510 --> 00:29:37,210 +مبتسم بشكل دائم، مبتسم بشكل دائم هو يعني هذا مؤشر + +405 +00:29:37,210 --> 00:29:40,950 +على أنه ليس كل ابتسامة هي تعبّل عن السعادة، ربما + +406 +00:29:40,950 --> 00:29:47,270 +يكون عنده Titanus، تمام؟فهذه نقطة كتير مهمة الان + +407 +00:29:47,270 --> 00:29:51,790 +كلمة invasive enzymes خلّيكم معايا انا فاندي خلية + +408 +00:29:51,790 --> 00:29:56,770 +و خلية مرتبطين في بعض في نسيج الان الارتباط هذا + +409 +00:29:56,770 --> 00:30:03,040 +بيجعل من الالأنسجة عائق أمام البكتيريا للاختراق، + +410 +00:30:03,040 --> 00:30:07,900 +لا تستطيع إنها تغزو مناطق بعيدة عن مكان تواجدها، + +411 +00:30:07,900 --> 00:30:12,060 +فاجتمع البكتيريا تنتج enzymes زي مثلا الكلاجينيز + +412 +00:30:12,060 --> 00:30:18,420 +والإلاستيز، الكلاجينيز والإلاستيز بيدوبوا + +413 +00:30:19,490 --> 00:30:23,410 +الفواصل ما بين الخلايا وبالتالي بيسمح للبكتيريا + +414 +00:30:23,410 --> 00:30:27,970 +انها تغزو الانسجار فمسموها invasive enzymes يعني + +415 +00:30:27,970 --> 00:30:32,090 +انزيمات تساعد البكتيريا في غزو الانسجار ووصل + +416 +00:30:32,090 --> 00:30:34,870 +الصورة ان فيها اندريجز توكسينز واخدنا مثلا لها + +417 +00:30:34,870 --> 00:30:38,550 +اللي استخدم تتانيه توكسين أو تتانوسبازمين واتكلمنا + +418 +00:30:38,550 --> 00:30:42,590 +عن invasive enzyme واخدنا مثل كولاجينيز and اللي + +419 +00:30:42,590 --> 00:30:48,180 +هو الاستيز نجي لل pigmentبعض المركبات البعض + +420 +00:30:48,180 --> 00:30:52,000 +البكتيريا ألوانها جميلة ألوانها جميلة تفيل + +421 +00:30:52,000 --> 00:30:55,480 +البكتيريا الخضرة هذه خضرة مزرقة أعتقد اللون يكون + +422 +00:30:55,480 --> 00:31:00,120 +أخضر مزرق بكتيريا اسمها Sodomonas aeruginosa + +423 +00:31:00,120 --> 00:31:04,820 +Sodomonas aeruginosa بتنتج pigment و اللي على + +424 +00:31:04,820 --> 00:31:08,020 +الجهة التانية اللون الأحمر هذه البكتيريا اسمها + +425 +00:31:08,020 --> 00:31:14,960 +Serratia marcesens لون أحمرالان بتتلاحظوا ملاحظة + +426 +00:31:14,960 --> 00:31:19,920 +نكية ان في اللون الأحمر اللون فقط موجود في + +427 +00:31:19,920 --> 00:31:24,740 +المستعمرات مش موجود في الطبق بينما في اللون + +428 +00:31:24,740 --> 00:31:30,650 +التانية منتشر في المستعمرات وموجود في وين؟فى الطبق + +429 +00:31:30,650 --> 00:31:35,390 +دلوقت عايش على ان water soluble و water insoluble + +430 +00:31:35,390 --> 00:31:40,050 +مين ال insoluble الحمرة اللى تأثيراتها مارس سنس + +431 +00:31:40,050 --> 00:31:46,290 +بينما السيدو موناس إيجينوزا water soluble هل هناك + +432 +00:31:46,290 --> 00:31:51,090 +فوائد لل pigment ولا مافيش فوائد البكيرة بتنتج بس + +433 +00:31:51,090 --> 00:31:56,090 +عشان تتلون يعنى تصبغ حاجة تشوفوا أحد الفوائد اللى + +434 +00:31:56,090 --> 00:31:59,010 +أخدنا أفهى عن السيدو موناس إيجينوزا أنها بتنتج في + +435 +00:31:59,010 --> 00:32:04,960 +الوسطتنتشر في الوسط وبترتبط في بعض المراكبات زي ال + +436 +00:32:04,960 --> 00:32:09,060 +iron بيصير ال iron هذا bound فاكرين ال bound + +437 +00:32:09,060 --> 00:32:12,880 +water؟ هل كان .. هل .. هل البكتيريا بتستفيد من ال + +438 +00:32:12,880 --> 00:32:17,520 +bound water؟ وإن ما بتستفيدش؟ الآن ال bound iron + +439 +00:32:17,520 --> 00:32:21,180 +اللي في الوسط هذا لو كان فيه بكتيريا أخرى في + +440 +00:32:21,180 --> 00:32:26,580 +المنطقة الآن لحظة هي مثلا في المنطقة هذه فيه + +441 +00:32:26,580 --> 00:32:30,420 +بكتيريا لكن هنا مافيش بكتيرياتخيلوا في استفيل + +442 +00:32:30,420 --> 00:32:33,640 +الكوكاس اوريس في المنطقة هذه، الاستفيل الكوكاس + +443 +00:32:33,640 --> 00:32:38,420 +اوريس بدها ايرون، فيه بدهم ايرون، لكن الايرون هذا + +444 +00:32:38,420 --> 00:32:42,840 +مرتبط، مرتبط مين؟ بالصبغة تبع ال .. ال .. ال .. ال + +445 +00:32:42,840 --> 00:32:43,340 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +446 +00:32:43,340 --> 00:32:43,500 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +447 +00:32:43,500 --> 00:32:44,460 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +448 +00:32:44,460 --> 00:32:45,000 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +449 +00:32:45,000 --> 00:32:47,180 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +450 +00:32:47,180 --> 00:32:47,200 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +451 +00:32:47,200 --> 00:32:47,360 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +452 +00:32:47,360 --> 00:32:51,810 +.. ال .. ال .. ال ..يعني بتجعل الحديد غير متعلمين + +453 +00:32:51,810 --> 00:32:56,970 +للآخرين، وسيلة من وسائل التنافس المخيفة، يعني + +454 +00:32:56,970 --> 00:33:01,390 +البشر مابيعرفوش يستخدم .. ينكروا هذا المقر أو .. + +455 +00:33:01,390 --> 00:33:04,230 +في زمان اتحادين بيعرفوا أكتر، بتعلموا من + +456 +00:33:04,230 --> 00:33:07,410 +البكتيريا، + +457 +00:33:07,410 --> 00:33:10,370 +يعني الآن زي واحد بيدي بالظبط مش بعمل .. بخش في + +458 +00:33:10,370 --> 00:33:14,160 +قاعة زي هذهوبروح بحط ورقة محجوز، محجوز، محجوز، + +459 +00:33:14,160 --> 00:33:17,620 +محجوز، تمام؟ كل اللي بيجوا بيلاقوا الورقة المحجوز + +460 +00:33:17,620 --> 00:33:21,220 +بيسحبوا عليهم إيه و بيطلعوا إلى الخارج، بيقدروا + +461 +00:33:21,220 --> 00:33:23,940 +يستفيدوا من الكراسي، معناه الكراسي موجودة، لكن أنا + +462 +00:33:23,940 --> 00:33:26,420 +وقت ما بدي باجي و بجد على كرسي، أي كرسي من الكراسي + +463 +00:33:26,420 --> 00:33:29,200 +هذا، فإذا البكتيريا وصلت لل iron، بتاخدوه و + +464 +00:33:29,200 --> 00:33:34,550 +بتستخدموه هناالبيجمنت في كل ميكروفيلة فائدة مختلفة + +465 +00:33:34,550 --> 00:33:39,530 +عن الآخر في بعض منها أساسي في الـ virulence، أساسي + +466 +00:33:39,530 --> 00:33:42,470 +في الـ virulence، يعني virulence فاكرة، المهم أنه + +467 +00:33:42,470 --> 00:33:47,010 +فيه pigments و فيه ألوان للبكتيرياسيداموناس + +468 +00:33:47,010 --> 00:33:50,570 +إيرجنوزا في ألوان أصفر وفي أخضر وفي أزرق وما + +469 +00:33:50,570 --> 00:33:56,810 +بينهما أصفر مخضر أخضر مصفر أزرق مخضر أخضر مصفر لكن + +470 +00:33:56,810 --> 00:33:59,910 +هاي ال pigments بتاعي هاي السيرات اللون الأحمر + +471 +00:33:59,910 --> 00:34:03,190 +القاني اللي بيشبه اللي حد كده بيسموه tomato juice + +472 +00:34:03,190 --> 00:34:07,690 +يعني عصير الطماطم اللون بتاعها إذا في عندي مجموعة + +473 +00:34:07,690 --> 00:34:11,750 +من المنتجات اللي بتنتجها الوقت إكسدوكسين invasive + +474 +00:34:11,750 --> 00:34:13,950 +enzymes وهي كان عنها pigments + +475 +00:34:16,250 --> 00:34:20,290 +انتيبايتكس تنتج البكتيريا هو ال .. ال .. ال .. ال + +476 +00:34:20,290 --> 00:34:21,930 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +477 +00:34:21,930 --> 00:34:21,950 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +478 +00:34:21,950 --> 00:34:23,450 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +479 +00:34:23,450 --> 00:34:23,730 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +480 +00:34:23,730 --> 00:34:24,110 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +481 +00:34:24,110 --> 00:34:33,010 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +482 +00:34:33,010 --> 00:34:33,650 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +483 +00:34:33,650 --> 00:34:33,690 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +484 +00:34:33,690 --> 00:34:33,750 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +485 +00:34:33,750 --> 00:34:33,850 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +486 +00:34:33,850 --> 00:34:33,970 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +487 +00:34:34,020 --> 00:34:37,780 +البنسينيين من فطر يعني اقصدوا ان البكتيريا .. + +488 +00:34:37,780 --> 00:34:40,060 +البكتيريا بتنتج .. أو بعض البكتيريا .. ال + +489 +00:34:40,060 --> 00:34:44,100 +actinomycetes أشهر مجموعة حكينا عنها مبرح .. لما + +490 +00:34:44,100 --> 00:34:47,920 +اتكلمنا عن المطر، تمام؟ و ان الرايحة اللي بنشتمها + +491 +00:34:47,920 --> 00:34:51,600 +أولة تشت الدنيا ناتجة عن ال actinomycetes، ال + +492 +00:34:51,600 --> 00:34:54,860 +actinomycetes من أشهر منتجات ال antibiotic في + +493 +00:34:54,860 --> 00:35:01,140 +الدنيا، تمام؟Bacteriocenes، هناك بعض المركبات لا + +494 +00:35:01,140 --> 00:35:05,540 +ترتقي نسميها Antibiotics، لكن نقدر نوصفها + +495 +00:35:05,540 --> 00:35:09,540 +Antibiotic-like substances، يعني مركبات تشبه الـ + +496 +00:35:09,540 --> 00:35:11,780 +Antibiotic، إيش يعني ممكن تشبهها؟ تشبهها + +497 +00:35:11,780 --> 00:35:17,440 +بالactions، ابتنتجها البكتيريا في أمعاقنا، حتى تحد + +498 +00:35:17,440 --> 00:35:21,630 +من نمو الآخرإحنا لو .. لو اتكلمنا عن البكتيريا + +499 +00:35:21,630 --> 00:35:25,730 +اللى موجودة في معايا الإنسان هي عبارة عن دوار أغمى + +500 +00:35:25,730 --> 00:35:30,270 +.. دوار أغمى تتنافس فيما بينها بأسلحة متنوعة + +501 +00:35:30,270 --> 00:35:34,150 +ومختلفة فعشانك فيه اتزال لإن كل واحد عارف قوة + +502 +00:35:34,150 --> 00:35:37,830 +الآخر .. عارف قوة الآخر من ضمن الأسلحة المستخدمة + +503 +00:35:37,830 --> 00:35:41,770 +في التوازن هو ال bacteriocenes اللي هنا بتشبه مين + +504 +00:35:41,770 --> 00:35:45,470 +ال antibiotics و هناك بعض البكتيريا بتنتج vitamins + +505 +00:35:45,470 --> 00:35:48,860 +و احنا قولنا لكم كل أحد فوائدال normal flora في + +506 +00:35:48,860 --> 00:35:52,060 +الأمعاق أو ال normal microbiota هو إنتاج بعض أنواع + +507 +00:35:52,060 --> 00:35:57,080 +من اللي هو ال vitamin z و ال vitamin k يبقى فيه + +508 +00:35:57,080 --> 00:36:02,700 +منتجات ضارة فيه منتجات جيدة فيه virulence factors + +509 +00:36:02,700 --> 00:36:06,040 +فيه toxins فيه أشياء مستغللة احنا في التعرف على ال + +510 +00:36:06,040 --> 00:36:11,540 +micro organisms الآن + +511 +00:36:11,540 --> 00:36:15,610 +نيجي لاستغلال كل هذه المعلومات اللي دراسناها من + +512 +00:36:15,610 --> 00:36:21,230 +أول المحاضرة اليوم ومبارح إلى توظيف هذه المعلومات + +513 +00:36:21,230 --> 00:36:26,850 +في المعمل artificial cultivation of bacteria يعني + +514 +00:36:26,850 --> 00:36:30,050 +الآن بدنا نمي البكتيريا بشكل صناعي طب ما هي بتنمو + +515 +00:36:30,050 --> 00:36:33,550 +في ال .. في البيئة و بتتكاتر في البيئة لكن انا بدي + +516 +00:36:33,550 --> 00:36:37,390 +نميها بشكل صناعي طبعا تنميت ان البكتيريا أكيد ليست + +517 +00:36:37,390 --> 00:36:43,430 +رفاهية وانما لغرض او لمجموعة أغراضمن مجموعة + +518 +00:36:43,430 --> 00:36:46,830 +الأغراض هذه أن نتعرف على البكتيريا لا أستطيع أن + +519 +00:36:46,830 --> 00:36:50,350 +أتعرف على البكتيريا بشكل دقيق بيسموها ال golden + +520 +00:36:50,350 --> 00:36:55,310 +standard يعني أفضل وسيلة للتعرف على البكتيريا هو + +521 +00:36:55,310 --> 00:37:00,510 +تنميتها in pure culture بمزرعة نقية ومن ثم دراسة + +522 +00:37:00,510 --> 00:37:03,570 +خصائصها ايه كانت دراسة خصائصها gram positive ولا + +523 +00:37:03,570 --> 00:37:06,050 +gram negative اندولبوسطف ولا اندولبوسطف اللي هو ال + +524 +00:37:06,050 --> 00:37:10,710 +biochemical test اللي هتعلموهم في المعمليُطلَق على + +525 +00:37:10,710 --> 00:37:15,390 +الوسط المُستخدم في تنمية البكتيريا Medium و جمعها + +526 +00:37:15,390 --> 00:37:20,630 +Medium أووساط Media Medium وسط It's a liquid or + +527 +00:37:20,630 --> 00:37:25,490 +solid mixture خلطة سائلة أو صلبة designed to + +528 +00:37:25,490 --> 00:37:30,090 +support the growth of microorganisms usually + +529 +00:37:30,090 --> 00:37:33,590 +called broth of liquid إذا كانت سائر بنسميها broth + +530 +00:37:33,590 --> 00:37:38,720 +و broth معناها ملق شورب يعني تمام؟و usually called + +531 +00:37:38,720 --> 00:37:42,880 +agar if solid بنسميها agar لإن اصلا نضيف مادة + +532 +00:37:42,880 --> 00:37:46,980 +اسمها agar agar تمام بتخليها بالظبط solid إذن في + +533 +00:37:46,980 --> 00:37:51,480 +عندي شكلين أساسيين في الوسط الذراعية liquid and + +534 +00:37:57,010 --> 00:38:02,110 +اللي هو يعني بنضيف كمية أجار، بالضبط، حتى يجعل + +535 +00:38:02,110 --> 00:38:07,290 +قوامه يعني semi-solid، مش solid و لا هو liquid لغة + +536 +00:38:07,290 --> 00:38:11,110 +بواحد، وهو حقيقة فحص ال motility، فحص ال motility، + +537 +00:38:11,110 --> 00:38:16,920 +هالكلة وانا لوحدي فعلا، طيبالأوساط الزراعية هناك + +538 +00:38:16,920 --> 00:38:20,280 +تصنيفات إلا بشكل كبير يعني .. يعني .. يعني .. خدنا + +539 +00:38:20,280 --> 00:38:24,580 +نقول نجا نصنيفها بأكتر من وسيلة من ضمن الوسائل + +540 +00:38:24,580 --> 00:38:28,580 +according to basic ingredients المحتوى ال .. ال .. + +541 +00:38:28,580 --> 00:38:31,260 +ال .. ال .. أو المحتويات اللي موجودة في الوساطة + +542 +00:38:31,260 --> 00:38:34,880 +فمثلا عندنا حاجة Minimal Essential Growth Medium + +543 +00:38:34,880 --> 00:38:38,860 +ومن اسمه Minimal Essential Growth Medium + +544 +00:38:43,820 --> 00:38:48,340 +بمعنى أخر، الحد الأدنى من المطلوبات للنمو، يعني + +545 +00:38:48,340 --> 00:38:51,620 +ينتلق هذا الوسط، الحد الأدنى من المطلوبات للنمو، + +546 +00:38:51,620 --> 00:38:57,740 +مش ال enrichment main يعني، + +547 +00:38:57,740 --> 00:39:03,660 +بده شرح هذا، نركز مع بعض، فاكرين لما اتكلمنا عن + +548 +00:39:03,660 --> 00:39:07,280 +البكتيريا وسط ال wall، كنا البكتيريا اللى بتنزل في + +549 +00:39:07,280 --> 00:39:13,110 +الحمام، بتنزل في الماء الحلوة، أمام؟و الميه العذبة + +550 +00:39:13,110 --> 00:39:17,690 +مافيش فيها ملح بكميات كافية تعمل balance بين ال .. + +551 +00:39:17,690 --> 00:39:20,390 +بالضغط الاثموذي اللي تبقى على الخلية و ال .. و + +552 +00:39:20,390 --> 00:39:24,090 +البيئة وبالتالي بيكون البكتيريا under stress وفي + +553 +00:39:24,090 --> 00:39:28,230 +البيئة البكتيريا معظم الأوقات بتكون under stress + +554 +00:39:28,230 --> 00:39:32,110 +ومدام under stress لما انا ابتدأ .. اقولكوا بلاش + +555 +00:39:32,110 --> 00:39:34,310 +في رسم الإنسان .. لما انا ابتدأ أقل من رسم الإنسان + +556 +00:39:34,310 --> 00:39:37,310 +مش في حرب دائرة أساسا احنا بين .. بين البكتيريا و + +557 +00:39:37,310 --> 00:39:40,190 +بين ال immune system فممكن البكتيريا تكون under + +558 +00:39:40,190 --> 00:39:40,830 +stress + +559 +00:39:43,680 --> 00:39:47,680 +طبعا باللغة العربية هتكون كلمة مجروحة لكن مقصود + +560 +00:39:47,680 --> 00:39:53,780 +فيها أنه مش في صحتها يعني مش بأفضل صورها عشان أنا + +561 +00:39:53,780 --> 00:40:00,720 +أنميها لازم أحطها في وسط غني جدا و يساعدها على + +562 +00:40:00,720 --> 00:40:06,220 +الاستجمام من المصائب اللي ألمت فيها و من ال stress + +563 +00:40:06,220 --> 00:40:09,260 +اللي كان اتوقعله و هذا الوسط غالبا بيسميه احنا ايه + +564 +00:40:09,260 --> 00:40:14,240 +enrichment mainlyفي المستقبل هنعيد تعريفه بما + +565 +00:40:14,240 --> 00:40:17,920 +يتلقى مع موضوعات أخرى لكن هذا لأن الحد الأدنى اللي + +566 +00:40:17,920 --> 00:40:23,040 +هنفهمه أنها أوساط غنية جدا تستخدم لإنعاش بعض + +567 +00:40:23,040 --> 00:40:29,260 +الميكروبات اللي هي بتكون under stress نيجي للجزء + +568 +00:40:29,260 --> 00:40:32,180 +أو النوع التالت اللي هو ال selective medium + +569 +00:40:35,200 --> 00:40:41,000 +عبارة عن مصدر استخدمناه لانتقاء نوع معين من + +570 +00:40:41,000 --> 00:40:46,160 +البكيري كيف ان انتقاء نوع معين؟ تخيلوا لو انا بدى + +571 +00:40:46,160 --> 00:40:51,380 +احكم على غذاء انه صالح للاستخدام او غير صالح + +572 +00:40:51,380 --> 00:40:55,800 +للاستخدام والمواصفة الفلسطينية للأغذية او لهذا + +573 +00:40:55,800 --> 00:40:59,920 +الغذاء بتقول يجب ان يكون هناك اكثر من مئة + +574 +00:40:59,920 --> 00:41:06,090 +staphylococcus aureusطيب يبقى أنا الآن مضطر أعد + +575 +00:41:06,090 --> 00:41:10,810 +فقط مين؟ طب هو يمكن يكون ما يكون بعد أخرى مسموح + +576 +00:41:10,810 --> 00:41:14,770 +فيها، أنا مابديها، المواصفة بتقول اعد اللي جداش + +577 +00:41:14,770 --> 00:41:22,330 +فيه استفيد الكوكاس or yes فلازم أصمم مواصف لا ينمي + +578 +00:41:22,330 --> 00:41:24,650 +إلا باستفيد الكوكاس or yes + +579 +00:41:28,120 --> 00:41:32,820 +يعني باقتصاد كله لأننا عارفه بنقول هو وسط يستخدم + +580 +00:41:32,820 --> 00:41:38,780 +للنمو والانتقاء للبكتيريا نجلة الأخيرة اللي هو ال + +581 +00:41:38,780 --> 00:41:42,220 +differential أعطاني مره معناها مصطلح differential + +582 +00:41:42,220 --> 00:41:47,360 +ال differential معناها تميز يميز صح ال + +583 +00:41:47,360 --> 00:41:52,240 +differential media هي وسط ت differentiate between + +584 +00:41:52,240 --> 00:41:54,940 +two similar organisms او بتعطيكم مثال + +585 +00:42:03,350 --> 00:42:07,390 +السلمونيلا والإيشريشيا كولاي التنتين انترو + +586 +00:42:07,390 --> 00:42:11,230 +بكترياسي التنتين انترو بكترياسي والتنتين مكون + +587 +00:42:11,230 --> 00:42:13,990 +موجودين في ال intestine يعني موجودين في عينة ال + +588 +00:42:13,990 --> 00:42:17,830 +stool عينة الكولا إذا أكرمكم الله إذا بدي أعزل + +589 +00:42:17,830 --> 00:42:21,630 +السلمونيلا من الوسط بروح بحطها في وسط اسم الماكنكي + +590 +00:42:21,630 --> 00:42:26,670 +الماكنكي بيميزلي بين two types of microorganisms + +591 +00:42:26,670 --> 00:42:32,020 +بين lactose fermenter و لاكتوز فرمنترواحدة أعطاها + +592 +00:42:32,020 --> 00:42:36,440 +لون أحمر وواحدة ماعطاهاش لون خالص دي في وسط عمل + +593 +00:42:36,440 --> 00:42:41,580 +differentiation عمل differentiation فدي بقى لكوا + +594 +00:42:41,580 --> 00:42:48,300 +رقم Z وD من أعظم الإنجازات في مختبرات ال + +595 +00:42:48,300 --> 00:42:53,040 +microbiology ومن كتر ما هم مهمين غالبا بيلاجيش + +596 +00:42:53,040 --> 00:42:56,460 +selective لحاله في ال medical و differential لحاله + +597 +00:42:56,460 --> 00:43:00,870 +فبيلاجي الوسط نفسه بيمتلك ايه الصفتانبسميه + +598 +00:43:00,870 --> 00:43:05,190 +selective differential نرجع للمقنكي المقنكي + +599 +00:43:05,190 --> 00:43:09,630 +selective بحيث انه وضع فيه بعض المركبات بحيث لا + +600 +00:43:09,630 --> 00:43:12,070 +ينمي ال gram booster فهو selective ده ال gram + +601 +00:43:12,070 --> 00:43:16,270 +negative وإذا استطيع ان يميز بين lactose fermenter + +602 +00:43:16,270 --> 00:43:18,710 +و لا lactose fermenter فهو differential + +603 +00:43:23,950 --> 00:43:26,690 +كمان الوضع ده اللي استخدمناه لعدل Staphylococcus + +604 +00:43:26,690 --> 00:43:30,070 +aureus سميناه selective صح؟ برضه differential لأنه + +605 +00:43:30,070 --> 00:43:34,350 +للأسف خلناه selective لل Staphylococci بس مش لل + +606 +00:43:34,350 --> 00:43:37,610 +Staphylococcus aureus فعشان نعدل Staphylococcus + +607 +00:43:37,610 --> 00:43:41,370 +aureus خلناه differential Staphylococcus aureus + +608 +00:43:41,370 --> 00:43:46,290 +بتبين صفرة وما قعداها ببين حمر أحمر فصار selective + +609 +00:43:46,290 --> 00:43:51,850 +andأعتقد ان هناك الصورة بتكون واضحة تماما ان انا + +610 +00:43:51,850 --> 00:43:55,410 +استطيع أن أصمم أوصات زراعية في ال microbiology و + +611 +00:43:55,410 --> 00:43:59,650 +انا بقولك كده مرة هذا التصميم يجب ان لا يقتصر على + +612 +00:43:59,650 --> 00:44:03,170 +من صممه كمان انتوا عندكوا المقدر على التفكير و + +613 +00:44:03,170 --> 00:44:06,070 +عندكوا المقدر على انكوا لو طلبت منا في يوم من + +614 +00:44:06,070 --> 00:44:09,870 +الأيام ان نعمل modification او ممكن نفكر احنا في + +615 +00:44:09,870 --> 00:44:15,120 +modification او نخترع أوصات جديدةفي مقدرتنا و بس + +616 +00:44:15,120 --> 00:44:19,560 +بدنا نقضي وقت أطول في دراسة المعلومات المتعلقة + +617 +00:44:19,560 --> 00:44:23,780 +ببعض ال micro organism حتى نستطيع نوفر لها الصفات + +618 +00:44:23,780 --> 00:44:26,140 +المناسبة نكمل المرة 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+00:00:21,700 --> 00:00:24,280 +بسم الله الرحمن الرحيم نكمل ما بدأناه في ال + +2 +00:00:24,280 --> 00:00:28,660 +bacterial physiology المحاضرة السابقة تحدثنا عن + +3 +00:00:28,660 --> 00:00:32,380 +بعض العوامل بتأثر على نمو البكتيريا من nutrient و + +4 +00:00:32,380 --> 00:00:36,640 +من pH و من حرارة and osmotic pressure الاخرى و في + +5 +00:00:36,640 --> 00:00:39,820 +الآخر المحاضرة اتطرقنا لل doubling time او زمان + +6 +00:00:39,820 --> 00:00:43,280 +الانقسام و simple binary equation اليوم هتطلع لقصة + +7 +00:00:43,280 --> 00:00:47,900 +حياة البكتيريا كلنا كبشر إن قصص مختلفة و متنوعة + +8 +00:00:47,900 --> 00:00:55,020 +لكن تنتهي نهاية واحدة بالموتقصص غير هيك البكتيريا + +9 +00:00:55,020 --> 00:00:59,580 +القصة اللى مالكه على الصبورة الآن هي القصة + +10 +00:00:59,580 --> 00:01:05,480 +المشتركة لكل أنواع البكتيريا عندما تقع البكتيريا + +11 +00:01:05,480 --> 00:01:11,060 +في غذاء او في اي ديقة فيها مواد غذائية لا تبدأ + +12 +00:01:11,060 --> 00:01:16,160 +بالانقسام مباشرة وانما تدرس + +13 +00:01:16,160 --> 00:01:22,330 +مكونات الوسطوبالتالي تحتاج إلى فترة تعمل + +14 +00:01:22,330 --> 00:01:25,650 +adaptation بتجهز ال enzyme system تبعها واحنا + +15 +00:01:25,650 --> 00:01:28,550 +حكينالكم في السابق قولنالكم مثلا لو كان في جليكوز + +16 +00:01:28,550 --> 00:01:33,090 +ولاكتوز وكان فينا starch ويقادر على تناول هذه + +17 +00:01:33,090 --> 00:01:38,070 +العطر مثلها ده ستقوم بتجهيز ال enzyme system بحيث + +18 +00:01:38,070 --> 00:01:41,390 +انه تقول انه أنول أوفر للطاقة وفي دل الحالة هو + +19 +00:01:41,390 --> 00:01:45,340 +مين؟ الجليكوزعند هذا النقطة لازم نجرب و نتكلم + +20 +00:01:45,340 --> 00:01:49,380 +مصطلحين مهمين جدا مش مطلوبين على السبورة ال enzyme + +21 +00:01:49,380 --> 00:01:53,460 +ممكن نقسمهم احنا الى constitutive enzymes و كلمة + +22 +00:01:53,460 --> 00:01:56,320 +constitutive enzymes يعني enzymes that are + +23 +00:01:56,320 --> 00:02:02,760 +produced as long as the cell is growing او living + +24 +00:02:02,760 --> 00:02:08,120 +يعني بمعنى اخر ان الانزيمات هتنتج طالما الخلية حية + +25 +00:02:08,120 --> 00:02:11,740 +يعني مافيش عليها control مافيش عليها control بمعنى + +26 +00:02:11,740 --> 00:02:17,940 +اخر دايما موجودةبينما الـ induced enzymes they + +27 +00:02:17,940 --> 00:02:20,420 +will not be produced unless their substrate is + +28 +00:02:20,420 --> 00:02:24,500 +there يعني لما يصير فيه substrate لهم، فيه داعي، + +29 +00:02:24,500 --> 00:02:28,660 +فيه برتقال، بنجيب سكين من المطبخ مافيش برتقال، + +30 +00:02:28,660 --> 00:02:32,040 +يجيب خل السكين في المطبخ وصي الصورة، هذا اسمه اسمه + +31 +00:02:32,040 --> 00:02:38,100 +induced enzyme مين الأوفر الطاقة قلناها؟ ال + +32 +00:02:38,100 --> 00:02:41,260 +constitutive لأنه موجود، it is already there يعني + +33 +00:02:41,260 --> 00:02:47,520 +سواء شئ ما بينا، هو موجودوبتبدأ البكتيريا بالتغذية + +34 +00:02:47,520 --> 00:02:54,020 +لكن تنمو وتزداد حجما اللي قدامي في ال quick curve + +35 +00:02:54,020 --> 00:02:56,840 +هنا على الصبورة اللي بتلاحظه بالخط الأحمر اللي هو + +36 +00:02:56,840 --> 00:03:01,880 +ال lag phase من zero time إلى time معين ماكان فيه + +37 +00:03:01,880 --> 00:03:04,540 +زيادة في عدد البكتيريا ربما يكون فيه زيادة في + +38 +00:03:04,540 --> 00:03:11,560 +الحجم لكن مافيه زيادة في العددبعد ذلك بدأت مرحلة + +39 +00:03:11,560 --> 00:03:15,740 +أخرى من الانقسام بالخط الأزرق أو اللول الأزرق صار + +40 +00:03:15,740 --> 00:03:22,760 +الانقسام 2 4 8 16 32 إيش بنسمي هذا؟ بنسميه زيادة + +41 +00:03:22,760 --> 00:03:27,300 +لغاريثمية أو زيادة قصية إيه exponential أو + +42 +00:03:27,300 --> 00:03:31,460 +logarithmic increase in number وبالتالي تتضاعف في + +43 +00:03:31,460 --> 00:03:36,800 +عداد اللي هو ال bacteria الان إلى حد معينالبكتيريا + +44 +00:03:36,800 --> 00:03:41,400 +طبعا المقول ده في اناء مغلق، الاناء المغلق في كمية + +45 +00:03:41,400 --> 00:03:45,520 +محدودة من مين؟ من ال nutrients وبالتالي ال + +46 +00:03:45,520 --> 00:03:49,700 +nutrient بتبدأ بالنفاذ هذا واحد، رقم اتنين ال pH + +47 +00:03:49,700 --> 00:03:52,120 +تبقى ال media as a result of the metabolic + +48 +00:03:52,120 --> 00:03:56,540 +processes of the bacteria will decrease، بنزل ال + +49 +00:03:56,540 --> 00:04:00,880 +pH ويصير acidic ال accumulation of waste products + +50 +00:04:00,880 --> 00:04:06,430 +بتزيد، اللي هو تراكم المخلفات تبع نمو البكتيرياال + +51 +00:04:06,430 --> 00:04:09,830 +Oxygen إذا كان البكتيريا بهذه ال Oxygen قد يقل في + +52 +00:04:09,830 --> 00:04:14,930 +الوسط لأنك عندي مجموعة من العناصر بدأت إيه؟ خلّيني + +53 +00:04:14,930 --> 00:04:21,110 +أقول بطرة متالية للبكتيريا فصار عندي بعض البكتيريا + +54 +00:04:21,110 --> 00:04:25,230 +بتموت و البعض الأخر بينقسم و لو كنا في أي مجتمع في + +55 +00:04:25,230 --> 00:04:29,490 +الدنيا عدله فيها يساوي عد المواليد لن يكون هناك + +56 +00:04:29,490 --> 00:04:34,090 +زيادة في العدد انظروا إلى الخط الأخضرالخط الأكبر + +57 +00:04:34,090 --> 00:04:38,590 +اللي ماناها دلوقتي بالمقابل في time فترة زمنية + +58 +00:04:38,590 --> 00:04:42,890 +الفترة الزمنية هذه بتقول إنه لا يدد زيادة في العدد + +59 +00:04:42,890 --> 00:04:47,150 +لحد معينة مثلا منها مرحلة الثبات أو stationary + +60 +00:04:47,150 --> 00:04:53,330 +phase كل ما ذكرناه من ظروف قلنا ساقت فأدت إلى ال + +61 +00:04:53,330 --> 00:04:59,390 +stationary phase عندما تزداد سوء بيصير عدله فياه + +62 +00:04:59,390 --> 00:05:04,540 +أكبر من عدل الانقساماتو بالظبط و الآن بيبدأ العلبة + +63 +00:05:04,540 --> 00:05:08,120 +أو ال number بتباعها to decline او بنسمي decline + +64 +00:05:08,120 --> 00:05:11,960 +phase او بنسمي death phase اللي هو اللون الزهري + +65 +00:05:11,960 --> 00:05:15,820 +هذا اللي موجود أمامك و إذا فينا أربع مراحل بنرجعهم + +66 +00:05:15,820 --> 00:05:19,160 +مع بعض على السطورة اللي هم ال lag phase وهو لا + +67 +00:05:19,160 --> 00:05:21,760 +يولد فيه increase انما يولد فيه مرحلة تحضير و + +68 +00:05:21,760 --> 00:05:25,280 +adaptation ال logarithmic phase وهو المرحلة الأكبر + +69 +00:05:25,280 --> 00:05:28,840 +في مرحلة النمو ال stationary مرحلة ثبات و مرحلة + +70 +00:05:28,840 --> 00:05:33,300 +أخيرة اللي هي decline او death phaseقصة حياة أي + +71 +00:05:33,300 --> 00:05:37,920 +بكتيريا غالبا لا تتعدى هذه الخطوط الفترة الزمنية + +72 +00:05:37,920 --> 00:05:42,180 +قد تطول قد تقصر لكن في الآخر الشكل اللي قدامنا و + +73 +00:05:42,180 --> 00:05:44,820 +المراحل اللي قدامنا هي اللي هتسود في هذه العملية + +74 +00:05:44,820 --> 00:05:50,040 +وسط الصورة أجر ده تبقوا معايا أهلا لنقطة كتير مهمة + +75 +00:05:50,040 --> 00:05:55,460 +بدنا نستفيد استفادة طبية فاكرين ال gram stain؟ كنا + +76 +00:05:55,460 --> 00:05:59,100 +نقول دي gram stain gram بسطف و gram negative الفرق + +77 +00:05:59,100 --> 00:06:02,650 +بينهم مبني على ال cell wallالأقل لما بيقول + +78 +00:06:02,650 --> 00:06:06,850 +البكتيريا بتموت أو في وضع سيء، بقى لكوا ال cell + +79 +00:06:06,850 --> 00:06:10,330 +wall هو الجدولة الأسمنتي، بده يقعد ثانوية لما + +80 +00:06:10,330 --> 00:06:14,270 +يتحلق؟ ولا يتحلق على طول؟لذلك لو حوالنا نصبغ + +81 +00:06:14,270 --> 00:06:19,110 +بكتيريا بال gram stain وهي تموت، وهي تتحلل، لو + +82 +00:06:19,110 --> 00:06:21,930 +كانت ال gram positive هتبين ال gram negative لإن + +83 +00:06:21,930 --> 00:06:27,870 +السلوك تبعها بدأ يتحلل، إذا بنفهم أن أفضل منطقة و + +84 +00:06:27,870 --> 00:06:32,270 +أفضل جزئي نعمل فيها gram stain هي ال logarithmic + +85 +00:06:32,270 --> 00:06:34,850 +phase اللي هي معلنة باللون الأزرق على اللوحة اللي + +86 +00:06:34,850 --> 00:06:40,780 +هي أمامكليش؟ عشان كمان مرة البكتيريا بتكون في أوج + +87 +00:06:40,780 --> 00:06:43,820 +انقسامها أفضل ما يكون ال cell wall بيكون في هذه + +88 +00:06:43,820 --> 00:06:47,300 +المنطقة طبعا في ال industrial microbiology أو في + +89 +00:06:47,300 --> 00:06:49,960 +ال microbiology الصينية قد يكون ده كلام صحيح يعني + +90 +00:06:49,960 --> 00:06:53,280 +مش هذه الفترة هي المفضلة لدينا ممكن تكون فترات + +91 +00:06:53,280 --> 00:06:57,320 +أخرى مفضلة لكن احنا معنيين بهذه الفترة + +92 +00:07:00,890 --> 00:07:03,010 +صار عندنا فكرة عن ال growth نتكلم شوية عن ال + +93 +00:07:03,010 --> 00:07:05,830 +metabolism و ال metabolism احنا كده مرة هنا + +94 +00:07:05,830 --> 00:07:09,230 +مابنناقش biochemistry مابننرجع كل ال biochemistry + +95 +00:07:09,230 --> 00:07:14,010 +و انما بدنا نستغل بعض المعلومات في ال metabolism + +96 +00:07:14,010 --> 00:07:18,570 +حتى نستطيع من خلالها نتعرف على البكتيري يعني ال + +97 +00:07:18,570 --> 00:07:21,910 +metabolism هنا بغرض مين؟ التعرف على البكتيري + +98 +00:07:21,910 --> 00:07:26,320 +energy metabolismهتكلم عنه شوية هتكلم عن ال + +99 +00:07:26,320 --> 00:07:28,840 +medical important metabolic processes بمعنى + +100 +00:07:28,840 --> 00:07:32,940 +العمليات اللي لها علاقة بال medicine بال medical + +101 +00:07:32,940 --> 00:07:36,180 +microbiology تخيلوا بكتيريا as a waste product + +102 +00:07:36,180 --> 00:07:42,140 +تمتش تكسين أكيد التكسين هذا هيأثب عليه تمام؟ و زي + +103 +00:07:42,140 --> 00:07:45,080 +أي metabolism في الدنيا إما anabolic process أو + +104 +00:07:45,080 --> 00:07:49,960 +catabolic process إما عمليات بناء أو عملياتتعالوا + +105 +00:07:49,960 --> 00:07:53,720 +نقعد هذه اللوحة اللي أمامكوا اللوحة كتير جدا مهمة + +106 +00:07:53,720 --> 00:07:58,440 +لنا و اليوم يجب أن نؤكد على الفروقات الثلاث بين + +107 +00:07:58,440 --> 00:08:01,780 +الثلاث عناصر اللي موجودة أمامنا aerobic + +108 +00:08:01,780 --> 00:08:04,520 +respiration, anaerobic respiration and + +109 +00:08:04,520 --> 00:08:09,980 +fermentation التلاتة هي عمليات قد تقوم بها + +110 +00:08:09,980 --> 00:08:14,840 +البكتيريا وبالتالي بنميز بينهم التلاتة الأولى هي + +111 +00:08:14,840 --> 00:08:17,860 +الوحيدة اللي بتستهلك ال oxygen اللي هي aerobic + +112 +00:08:17,860 --> 00:08:23,250 +respirationبينما ال anaerobic respiration و ال + +113 +00:08:23,250 --> 00:08:27,150 +fermentation بتموا في غياب ال oxygen، طب كيف بنا + +114 +00:08:27,150 --> 00:08:30,810 +نميز بينهم من ناحية biochemistry؟ الأولى اللي هي + +115 +00:08:30,810 --> 00:08:34,710 +ال aerobic respiration، ال oxygen is the final + +116 +00:08:34,710 --> 00:08:38,190 +electron acceptor، + +117 +00:08:38,190 --> 00:08:39,110 +أظن واضحة النقطة هذه + +118 +00:08:43,420 --> 00:08:48,340 +استفادة من الكربوهيدرات بتتم في وجود الأكسجين one + +119 +00:08:48,340 --> 00:08:54,260 +molecule will produce 36 ATPs نيجي الآن لل + +120 +00:08:54,260 --> 00:08:57,260 +Anaerobic Respiration و ال fermentation كيبنا نميز + +121 +00:08:57,260 --> 00:09:00,560 +بينهم و خاصة إنه في ورية شبه إنه بتتموا في غياب + +122 +00:09:00,560 --> 00:09:04,720 +الأكسجين ال Anaerobic Respiration ال final + +123 +00:09:04,720 --> 00:09:08,380 +electron acceptor بيكون inorganic compound مركّب + +124 +00:09:08,380 --> 00:09:12,960 +غير عضوي غير الأكسجينInorganic compound غير ال + +125 +00:09:12,960 --> 00:09:16,900 +Oxygen بينما ال fermentation بيكون ال final + +126 +00:09:16,900 --> 00:09:20,960 +electron acceptor هو organic compound وصلت الصورة + +127 +00:09:20,960 --> 00:09:25,740 +Oxygen للأولى، Inorganic compound للتانية، تلاتة، + +128 +00:09:25,740 --> 00:09:29,620 +أكتر واحد more efficient أو the most efficient + +129 +00:09:29,620 --> 00:09:32,300 +اللي هي aerobic respiration، the least efficient + +130 +00:09:32,300 --> 00:09:38,710 +اللي هي ال fermentation هي الأقل انتجة للطاقةكيف + +131 +00:09:38,710 --> 00:09:41,650 +نستغل وهذه اللوحة اللي أمامنا وكتير مهمة نضلنا + +132 +00:09:41,650 --> 00:09:46,110 +مركزين عليها طوال الوقت حتى نستطيع أن نفهم ماذا + +133 +00:09:46,110 --> 00:09:51,890 +يعني بهذه اللوحة احنا في ال micro biology قد يظن + +134 +00:09:51,890 --> 00:09:54,710 +البعض انه كون الميكروبات دقيقة و احنا ممكن + +135 +00:09:54,710 --> 00:09:59,010 +المحاضرة مش المواد اللي جابلها تحدثنا عن استخدامات + +136 +00:09:59,010 --> 00:10:02,550 +الميكروسكوبات يمكن احنا اقل الناس اللي بنستخدم في + +137 +00:10:02,550 --> 00:10:07,620 +مجلة الميكروسكوبليش؟ طورنا أنظمة .. أنظمة الـ + +138 +00:10:07,620 --> 00:10:10,800 +detection بالتغيير في الألوان، بنوعها على مين؟ + +139 +00:10:10,800 --> 00:10:13,320 +بنوعها تغيروا في الـ pH فمثلا الصورة اللي قدامكوا + +140 +00:10:13,320 --> 00:10:17,620 +هذه اللون الأحمر هو اللون الأصلي للوسط زُنع + +141 +00:10:17,620 --> 00:10:21,500 +ببكتيريا فتحول إلى لون الأصفر، ليش تحول إلى لون + +142 +00:10:21,500 --> 00:10:25,560 +الأصفر؟ لأن البكتيريا عملت fermentation أو عملت + +143 +00:10:25,560 --> 00:10:30,460 +metabolism فخفضت ال pH واحنا حاطين pH indicator، + +144 +00:10:30,460 --> 00:10:34,450 +تمام؟فإيش يعني pH Indicator؟ يعني مُركّب يتغير + +145 +00:10:34,450 --> 00:10:38,930 +لونه بتغير ال pH فتغير من اللون الأحمر لللون + +146 +00:10:38,930 --> 00:10:41,590 +الأصفر إعرفنا إنه العينة أو ال .. أو ال .. + +147 +00:10:41,590 --> 00:10:45,830 +البكتيريا positive إيش يعني positive؟ يعني استطالت + +148 +00:10:45,830 --> 00:10:49,690 +أن تقوم بالمهمة إيش المهمة؟ to ferment أو to + +149 +00:10:49,690 --> 00:10:52,530 +metabolize على سبيل المثال ال dextrose أو ال + +150 +00:10:52,530 --> 00:10:56,970 +glucose طيب التنتين صفر صح؟ اللي في الصورة التنتين + +151 +00:10:56,970 --> 00:11:00,630 +صفر في فرق بينهم؟ في واحدة مطالعة غاز، شايفينها؟ + +152 +00:11:04,720 --> 00:11:08,160 +انطلعها غازي بعد الحكي انه في كمان شغل عملناها + +153 +00:11:08,160 --> 00:11:11,140 +detection في ال metabolism انه صار fermentation + +154 +00:11:11,140 --> 00:11:15,640 +positive or negative كيف عرفناها؟ بتغيير اللون في + +155 +00:11:15,640 --> 00:11:18,360 +كمان شغل تاني ممكن نعرفها انه fermentation with + +156 +00:11:18,360 --> 00:11:22,280 +gas ولا fermentation with no gas هاي كمان صفة + +157 +00:11:22,280 --> 00:11:26,160 +جديدة يعني بدل الفحص سرنانيا محصانة بدل الصفة + +158 +00:11:26,160 --> 00:11:31,250 +سرناندي صفة تانيةمثال آخر على الـ metabolic + +159 +00:11:31,250 --> 00:11:36,150 +process اللى بتساعدنا في عملية اللى هو التعرف على + +160 +00:11:36,150 --> 00:11:41,930 +البكتيريا فحص اسمه methyl red ال + +161 +00:11:41,930 --> 00:11:46,810 +methyl red هو عبارة عن برضه pH indicatorوسمى الفحص + +162 +00:11:46,810 --> 00:11:51,310 +باسم الـ PH Indicator المستخدم نظرا ليش؟ لأنه + +163 +00:11:51,310 --> 00:11:55,510 +بنضيف الـ Methyl Red مش مباشرة مع الميديا وإنما + +164 +00:11:55,510 --> 00:11:58,690 +بعد نمو البكتيريا يعني بمعنى أخر نزرع البكتيريا في + +165 +00:11:58,690 --> 00:12:04,030 +المبوبة تمام؟ فيها جلوكوز و بعد مرور 24 ساعة بنضيف + +166 +00:12:04,030 --> 00:12:09,730 +عليها الـ Methyl Red إذا تحول الوسط إلى اللون + +167 +00:12:09,730 --> 00:12:13,720 +الأحمر كما نرى في هذه الصورةده بيكون poster ماما + +168 +00:12:13,720 --> 00:12:19,460 +اللون الأحمر انه ال pH تبع ال media وصل لأقل من + +169 +00:12:19,460 --> 00:12:23,800 +أربعة و هم هتدرسوه بالتفصيل في المعمل ان شاء الله + +170 +00:12:23,800 --> 00:12:28,060 +و هتشتغلوا شغل عملي بإذن الله لكن في الآخر ميازت + +171 +00:12:28,060 --> 00:12:31,700 +بين بكتيريا methyl red poster و أخرى methyl red + +172 +00:12:31,700 --> 00:12:35,280 +ash negative اللون الأحمر poster و اللون الأصفر + +173 +00:12:35,280 --> 00:12:35,740 +negative + +174 +00:12:38,500 --> 00:12:42,080 +الـ Bogus Proscour هو نوع آخر من الفحوصات المعتمدة + +175 +00:12:42,080 --> 00:12:45,380 +على الـ metabolic processes هناك بعض البكتيريا + +176 +00:12:45,380 --> 00:12:51,880 +عندها metabolic pathway اسمه 2,3-butylene glycol + +177 +00:12:51,880 --> 00:12:59,960 +أحد ال precursors المميزة مركب اسمه أسيتوين وليس + +178 +00:12:59,960 --> 00:13:04,220 +أسيتون في فرق أسيتوين هو اسم تاني + +179 +00:13:04,220 --> 00:13:07,520 +أسيتالميثالكاربينول برضه هدرسه في المعمل إن شاء + +180 +00:13:07,520 --> 00:13:12,790 +اللههذا المُركّب إن موجد فبمؤشر على وجود الـ 2,3 + +181 +00:13:12,790 --> 00:13:17,110 +-butanyl glycol fermentation pathway إذا لم يوجد + +182 +00:13:17,110 --> 00:13:20,850 +فمعناه الحاجة أن البكتيريا لا تمتلك هذا الpathway + +183 +00:13:20,850 --> 00:13:25,650 +بعد ما بننمي البكتيريا بنضيف two compounds يعني + +184 +00:13:25,650 --> 00:13:29,990 +two detectors إذا اتحول الأنبوبة إلى لون الأحمر + +185 +00:13:29,990 --> 00:13:35,410 +بصي فيما اتحول إلى لون الأحمر نيجاتي إستعت من خلال + +186 +00:13:35,410 --> 00:13:36,330 +فحص + +187 +00:13:39,410 --> 00:13:43,050 +محرف على وجود metabolic pathway، وانا متكلم عن مين + +188 +00:13:43,050 --> 00:13:46,790 +احنا الآن في المحاضرة هاي؟ متكلم عن ال bacterial + +189 +00:13:46,790 --> 00:13:51,830 +physiology، عن ال growth and metabolismفاستغلنا ال + +190 +00:13:51,830 --> 00:13:55,570 +Metabolism في التفريق بين أنواع ال micro .. ال + +191 +00:13:55,570 --> 00:13:58,270 +micro organism و أول محاضرة .. إذا بترجعوا + +192 +00:13:58,270 --> 00:14:01,530 +المحاضرة الأولى هتتذكروا إن أنا قلتلكوا معلومة جدا + +193 +00:14:01,530 --> 00:14:04,490 +إن ما يميز ال micro organism هو ال metabolic + +194 +00:14:04,490 --> 00:14:08,970 +biodiversity التنوع في ال metabolism بكتيريا تتغذى + +195 +00:14:08,970 --> 00:14:12,450 +على الجلوكوز أخرى على الجلوكوز زاد ال lactose أخرى + +196 +00:14:12,450 --> 00:14:17,310 +على الجلوكوز زاد ال maltose و هكذا أنواع مختلفة من + +197 +00:14:17,310 --> 00:14:20,730 +ال substrateتستغل من قبل أنواع مختلفة من ال micro + +198 +00:14:20,730 --> 00:14:26,630 +organism حصل رابعا والصورة اللي أمامكوا واضحة كتير + +199 +00:14:26,630 --> 00:14:31,670 +أنه في لونين أزرق وأخضر ال original color للأنبوبة + +200 +00:14:31,670 --> 00:14:34,510 +هذي هو اللون الأخضر ال original color هو اللون + +201 +00:14:34,510 --> 00:14:38,830 +الأخضر إذا اتحول إلى اللون الأزرق فالفحص positive + +202 +00:14:38,830 --> 00:14:43,140 +هقولكوا كيف بتحولهذا الفحص معتبد على قدرة + +203 +00:14:43,140 --> 00:14:47,840 +البكتيريا عليها تستغل ال sodium citrate كمصدر + +204 +00:14:47,840 --> 00:14:51,520 +كربوني وحيد كمصدر كربوني وحيد مش كل البكتيريا + +205 +00:14:51,520 --> 00:14:55,100 +عندها المقدرة هذه أنواع قليلة من الميكروبات عندها + +206 +00:14:55,100 --> 00:14:58,900 +المقدرة هذه إذا بقدر أميز هذه الأنواع عن بعض + +207 +00:14:58,900 --> 00:15:02,580 +الأنواع الأخرى اللي ماعندهاش هذه المقدرة بحط + +208 +00:15:02,580 --> 00:15:06,560 +انبوبة فيها sodium citrate و فيها pH indicator + +209 +00:15:06,560 --> 00:15:12,140 +اسمه bromthamol blueإذا البكتيريا استهلكت .. + +210 +00:15:12,140 --> 00:15:17,060 +استهلكت .. ال .. ال sodium citrate هتستهلك معاه + +211 +00:15:17,060 --> 00:15:22,800 +مركب عضوي آخر بيرفع ال pH إلى أعلى، أعلى يعني + +212 +00:15:22,800 --> 00:15:30,170 +alkaline، لما يرتفع ال pH إلى alkalineبيتحول اللون + +213 +00:15:30,170 --> 00:15:32,790 +الاسمه bromethymol الـ pH Indicator اللي اسمه + +214 +00:15:32,790 --> 00:15:36,810 +bromethymol blue إلى من أخضر إلى أسرع وابتدا اللي + +215 +00:15:36,810 --> 00:15:40,310 +بيقول الفحص هي positive إذا بقى كما هو الفحص + +216 +00:15:40,310 --> 00:15:44,430 +negative هذا كان فحص اللي عارفينه الآن ال + +217 +00:15:44,430 --> 00:15:46,570 +carbohydrate fermentation test خدوا بالكم مش فحص + +218 +00:15:46,570 --> 00:15:51,980 +واحد ال carbohydrate fermentation testمش بس لأ مش + +219 +00:15:51,980 --> 00:15:56,500 +بس كمان هيك، لكل carbohydrate بقدر أعمل فحص، يعني + +220 +00:15:56,500 --> 00:15:59,820 +بعمل للجليكوز، لللاكتوز، للستاكروز، للمريتول، + +221 +00:15:59,820 --> 00:16:04,460 +للسوربيتول، لمجموعة كبيرة من السكريات، وكلها بقدر + +222 +00:16:04,460 --> 00:16:08,240 +أخد منها الصفتين، ال post fermentation زاد اللي هو + +223 +00:16:08,240 --> 00:16:13,710 +ال gas productionالثلاث بحثات التانية كانوا methyl + +224 +00:16:13,710 --> 00:16:17,910 +red, vox proscarin و citrate هنتقل إلى الرابع في + +225 +00:16:17,910 --> 00:16:21,230 +السلسلة اللي هم مجموعين مع بعض اللي اسمه Endolpist + +226 +00:16:29,830 --> 00:16:35,290 +الان فحص الاندول هو وسيلة للكشف عن مقدرة البكتيريا + +227 +00:16:35,290 --> 00:16:40,070 +على إنتاج إنزيم اسمه triptophanase، بيكسر مين هذا؟ + +228 +00:16:40,070 --> 00:16:43,790 +triptophan، تكسير لـ triptophan بينتج عنه مركب + +229 +00:16:43,790 --> 00:16:47,950 +اسمه اندولهذا لأن دول بتتم الكشف عنه باضافة برضه + +230 +00:16:47,950 --> 00:16:50,670 +بعض المُركّبات الكيميائية باسمها Kovacs reagents + +231 +00:16:50,670 --> 00:16:56,550 +إذا تكون ال red range حلقة الحمراء هذه إذا تكونت + +232 +00:16:56,550 --> 00:17:01,890 +فالفحص positive يبقى قدامي هنابصتف لأنه فيه red + +233 +00:17:01,890 --> 00:17:05,470 +ring ال red ring نتيجة على أنه المادة اللي بنضيفها + +234 +00:17:05,470 --> 00:17:10,630 +فيها كحول بتستخلص ال indol و المادة الأخرى الملونة + +235 +00:17:10,630 --> 00:17:14,550 +وبالتالي بتستخلصه و بترفعه وان على السطح فبتكون + +236 +00:17:14,550 --> 00:17:17,570 +red ring على السطح في ال negative إيش فيها نهاية + +237 +00:17:17,570 --> 00:17:23,050 +في ال negative؟ زي ما شايفين مافيش لون لون ال + +238 +00:17:23,050 --> 00:17:26,270 +reagent فقط اللي هتنضف فيهاالأنبوبة الأولى اللى + +239 +00:17:26,270 --> 00:17:30,430 +على الجهة هاى هي أنبوبة غير مزروعة أساسا للمقارنة + +240 +00:17:30,430 --> 00:17:33,190 +عشان نقدر نشوف انه صار نمو ولا ماصارش نمو في + +241 +00:17:33,190 --> 00:17:36,470 +الأنبوبة الأخرى انا كان فحص صاروا الأن اربعة غير + +242 +00:17:36,470 --> 00:17:39,410 +الأولة تبقوا من الأولة نهرج بيكربوا ديه صار عندي + +243 +00:17:39,410 --> 00:17:43,770 +اندول ميتيل ريد بوكوس بروسكوار وسايترين انا + +244 +00:17:43,770 --> 00:17:46,750 +الترتيب اللى في اللوحة مش هو الترتيب اللى انا + +245 +00:17:46,750 --> 00:17:49,950 +بدهيه الترتيب اللى انا ذاكرته كمرة اندول + +246 +00:17:56,130 --> 00:17:59,130 +بشكل سريع يا بنات بنرجع للأروحات الأربع اللي + +247 +00:17:59,130 --> 00:18:03,030 +شفناهم ونطلع كلها تحت صورة إيش مكتوبة أو الصورة + +248 +00:18:03,030 --> 00:18:06,370 +الأولى اللي هي إيشريشيا كولاي و إنتيروباكتر + +249 +00:18:06,370 --> 00:18:10,250 +positive and negative ال default إشريشيا كولاي ال + +250 +00:18:10,250 --> 00:18:11,730 +negative الإنتيروباكتر + +251 +00:18:26,040 --> 00:18:30,980 +يبجى انا عندى اربع فحاصات قارنت بين two organisms, + +252 +00:18:31,200 --> 00:18:34,560 +two similar organisms وقت انتنى طلعوا positive + +253 +00:18:34,560 --> 00:18:39,440 +لفحص الشريشة كلها طلعت positive لان دول ميذرد و + +254 +00:18:39,440 --> 00:18:42,320 +negative للتانين للتانين الباجين و ال enterovector + +255 +00:18:42,320 --> 00:18:45,660 +negative للأولانين التانين و positive للتانين + +256 +00:18:45,660 --> 00:18:50,780 +التانينطيب ألم يكن يكفينا فحص واحد، انقول سيترات + +257 +00:18:50,780 --> 00:18:54,980 +بسط و سيترات نيجاتيف؟ خد القاعدة اللى لازم احنا + +258 +00:18:54,980 --> 00:18:58,220 +يعني نكتبها بحروف من ده في ال diagnostic + +259 +00:18:58,220 --> 00:19:01,560 +microbiology او في ال medical the more test you + +260 +00:19:01,560 --> 00:19:05,040 +use the more accurate is your diagnosis يعني كل ما + +261 +00:19:05,040 --> 00:19:09,720 +زاد عال الفحصات اللى بمستخدمهم كل ما زاد دقة عملية + +262 +00:19:09,720 --> 00:19:10,900 +اللى هو التعريف + +263 +00:19:14,280 --> 00:19:16,340 +الـ differentiation فاكرينه لما كنت أقولكوا + +264 +00:19:16,340 --> 00:19:20,300 +سالمونيلا وشيجيلا و مميز منهم بال motility غالبا + +265 +00:19:20,300 --> 00:19:25,800 +نعم هو فحص مميز لكن لا نعتمد عليه لوحده كفحص واحد + +266 +00:19:25,800 --> 00:19:31,160 +و لا نشبه معاه فحصات أخرى نرجع و نكمل ال .. ال .. + +267 +00:19:31,160 --> 00:19:36,400 +ال MVIC ال MVIC اللي قدامكوا هو رموز لأربع فحصات + +268 +00:19:36,400 --> 00:19:41,670 +ال I in doll و ال M مثيل Red و ال V أو ال VPبوجرس + +269 +00:19:41,670 --> 00:19:45,270 +بروس كوار و ال C سيتيطر على ال I just to make it + +270 +00:19:45,270 --> 00:19:49,730 +قابل للنطق ان نقول Invict تمام؟ وشايفين في الجدول + +271 +00:19:49,730 --> 00:19:55,010 +التحت ان الشريشة كولاي positive positive negative + +272 +00:19:55,010 --> 00:19:58,390 +negative بينما ال enterobacter negative negative + +273 +00:19:58,390 --> 00:20:02,990 +positive positive اعتقد ان هذه المجموعة من + +274 +00:20:02,990 --> 00:20:07,310 +الفحوصات أثبتت لنا beyond reasonable doubt يعني + +275 +00:20:07,310 --> 00:20:11,980 +بدون أدنى شكإنه ال metabolic processes ال + +276 +00:20:11,980 --> 00:20:17,340 +biochemistry تبعة البكتيريا مهمة جدا في عملية + +277 +00:20:17,340 --> 00:20:22,060 +تمييز البكتيريا أعطيكوا توضيح كامل من ألفه إلى ياه + +278 +00:20:22,060 --> 00:20:27,580 +تكرر ال grams تان؟ قلنا جدش عظيم ال grams تان؟ + +279 +00:20:27,580 --> 00:20:30,640 +بشكل كبير قلنا عنه عظيم قلنا عنه إنه بميز بين + +280 +00:20:30,640 --> 00:20:34,160 +مجموعتين كبار gram بسط و gram negative روحنا لل + +281 +00:20:34,160 --> 00:20:36,460 +gram negative احنا اللي قاعدين نتكلم عن بكتيريا + +282 +00:20:36,460 --> 00:20:39,340 +مجهولة عزلناهالما عملتنا gramستين طلعت gram + +283 +00:20:39,340 --> 00:20:45,260 +نيجاتيف طلعت شكلها لجناها بسلاي فصار عندي انا ربع + +284 +00:20:45,260 --> 00:20:51,420 +من اربع ايش هم الاربعة؟ gram positive coexi و gram + +285 +00:20:51,420 --> 00:20:54,640 +negative coexi gram positive بسلاي و gram negative + +286 +00:20:54,640 --> 00:20:59,660 +بسلاي يبقى صار عندي انا واحد من اربع ربع هذا الربع + +287 +00:20:59,660 --> 00:21:04,080 +بتبقى اجسام كمان اجسام عندي اربع اصناف مش للحفظ + +288 +00:21:04,080 --> 00:21:09,000 +الكلام اللي بحكيه لان لكن للتوضيحانتيرو بكترياسي + +289 +00:21:09,000 --> 00:21:13,340 +family كبيرة ضخمة Sodomonas Acinetobacter و + +290 +00:21:13,340 --> 00:21:17,080 +Vibrium أربع أصناف طلعت البكتيريا تبعتي تابعة + +291 +00:21:17,080 --> 00:21:21,720 +لعائلة اسمها انتيرو بكترياسي الانتيرو بكترياسي + +292 +00:21:21,720 --> 00:21:27,270 +فيها 200 اسمكلهم بيمتلكوا نفس الصفة اللى انا قلت + +293 +00:21:27,270 --> 00:21:30,910 +عشان انا مش عارف انترو بكترياسي يعني تمتلك كلهم + +294 +00:21:30,910 --> 00:21:33,850 +المتان بيمتلكوا نفس الصفة انهم يكونوا نفس العائلة + +295 +00:21:33,850 --> 00:21:37,910 +لكن جوا أسماء مختلفين بدي اميزهم عن بعض من ضمن ال + +296 +00:21:37,910 --> 00:21:42,750 +انترو بكترياسي اشيريشيا كولاي و انترو بكتر بينقسم + +297 +00:21:42,750 --> 00:21:45,610 +الانترو بكترياسي الى lactose fermenter و من ال + +298 +00:21:45,610 --> 00:21:48,950 +lactose fermenter هدولة طلعتوا ضمن مين ضمن ال + +299 +00:21:48,950 --> 00:21:52,370 +lactose fermenter شوفوا قدش اوجه التشابه بينهم + +300 +00:21:52,370 --> 00:21:59,510 +ترجعوا بالجورلكتوز فرمنتر، جليكوز فرمنتر، أكسيدايز + +301 +00:21:59,510 --> 00:22:03,430 +نيجاتيف، فاكولتاتف أناقروك، كل هذه صفات مفتركة بين + +302 +00:22:03,430 --> 00:22:09,050 +الانتروباكترا وبين الاشريشيا كولا طب أوجه تشابهم + +303 +00:22:09,050 --> 00:22:12,770 +متعددة و كثيرة يبقى في حاجة لإن هنا الميزهم عن + +304 +00:22:12,770 --> 00:22:16,890 +بعضهم من البعض، مين الميز أنهيهم؟ال biochemical + +305 +00:22:16,890 --> 00:22:20,570 +reactions لم نستطع نميزهم لأ بجرامس تاني ولا + +306 +00:22:20,570 --> 00:22:23,350 +بتقنية مختلفة أخرى ميزناهم بال biochemical + +307 +00:22:23,350 --> 00:22:26,950 +reactions لذلك تزوغ مهم جدا من ما عملكم هذا الفصل + +308 +00:22:26,950 --> 00:22:32,330 +هيكون مهتم بإجراءها بفهم و إجراء هذه الفحوصات اللي + +309 +00:22:32,330 --> 00:22:36,490 +هنستخدمها لاحقا في ال diagnostic microbial بتعرف + +310 +00:22:36,490 --> 00:22:42,520 +على ال micro organismفي كمان بعض الفحصات الأخرى + +311 +00:22:42,520 --> 00:22:45,060 +الكثيرة المتنوعة أكثر من ما أنا صادقه في هذه + +312 +00:22:45,060 --> 00:22:49,160 +المحاضرة لكن أحدها حاجة اسمها hydrogen sulfide + +313 +00:22:49,160 --> 00:22:56,700 +production H2S الـ H2S غاز عادي من اللون لكن + +314 +00:22:56,700 --> 00:23:02,590 +رائحته كريهة تعرفوها وسموها رائحت الويب الفاسداللي + +315 +00:23:02,590 --> 00:23:05,470 +بنشم بعض الأحيان في الشوارع اللي يكون فيها مجالي + +316 +00:23:05,470 --> 00:23:09,790 +راكدة هادر و لونها أسود طبعا أنا قاعد بقول عادي من + +317 +00:23:09,790 --> 00:23:15,170 +اللون هو صح؟ عادي من اللون لكن لو تفاعل مع الحديد + +318 +00:23:15,170 --> 00:23:18,990 +و الحديد موجود بكثرة في ال .. في المياه العادية، + +319 +00:23:18,990 --> 00:23:22,770 +الأشياء المصادية و الأمور هذه حديد فبيرتبط مع + +320 +00:23:22,770 --> 00:23:26,770 +الحديد بكون لون أسود، بكون لون أسود فاحنا برضه كيف + +321 +00:23:26,770 --> 00:23:30,590 +بنكشف أن البكتيرا بتنتج أو بتنتجوش؟ بنحط في ال + +322 +00:23:30,590 --> 00:23:35,780 +media حديدمركبات حديد فإذا الغاز طلع بيتحول الوسط + +323 +00:23:35,780 --> 00:23:39,740 +اللامين إلى اللامض الأزرق في فحص تاني اسمه urease + +324 +00:23:39,740 --> 00:23:44,620 +لو دخلنا دورات حمامات مش منظمة فترة طويلة بنشم + +325 +00:23:44,620 --> 00:23:46,920 +رائحة الأمونيا من بعيد رائحة كارية الأمونيا + +326 +00:23:46,920 --> 00:23:51,420 +بالنسبالنا الأمونيا هذه ناتجة على أن بكتيريا + +327 +00:23:51,420 --> 00:23:54,620 +موجودة في الدورات كسرت ال urea الموجودة في ال + +328 +00:23:54,620 --> 00:23:59,390 +urine وطلعت منها الأمونيانفس الفكرة بنقدر نستغل + +329 +00:23:59,390 --> 00:24:02,990 +اللي احنا في المعمل بنجيب قنبوبة و فيها يوريا و + +330 +00:24:02,990 --> 00:24:07,510 +بنزرع البكتيريا و إذا البكتيريا كسرت ال يوريا + +331 +00:24:07,510 --> 00:24:10,710 +هتحولها لأمون ال ammonia alkaline حطينا في الوسط + +332 +00:24:10,710 --> 00:24:15,190 +pH indicator تحول اللون إلى لون آخر و بنقول + +333 +00:24:15,190 --> 00:24:20,330 +positive أو negative في هذه الحالة إذا كل أو معظم + +334 +00:24:20,330 --> 00:24:23,930 +الفحوصات اللي مرض علينا هل شفناها تحت ال + +335 +00:24:23,930 --> 00:24:28,030 +microscope ولا طلعنا عليها تغيير ألوانتدير ألوان + +336 +00:24:28,030 --> 00:24:31,350 +دي داجة هي طبعا هتكون مفضلة بالنسبالكوا لأنه + +337 +00:24:31,350 --> 00:24:34,830 +ألوانها كتير جذابة اللي بفكر أو بالأحرى اللي في + +338 +00:24:34,830 --> 00:24:38,170 +حاجات الباقية هو ال chemical طبعا احنا كمان هتطور + +339 +00:24:38,170 --> 00:24:42,310 +ليش؟ لإنه احنا شايفين الأنابيب ما اكبرهم اللي بنحط + +340 +00:24:42,310 --> 00:24:47,270 +فيهم كبارلما نصل لمرحلة معينة نفهم ال .. ال .. ال + +341 +00:24:47,270 --> 00:24:50,310 +.. الانظمة كلها ال .. ال .. الفحصات البرجمية + +342 +00:24:50,310 --> 00:24:57,090 +هنسخطهم نزغرهم و نحط لعشرين فحص في سلسلة واحدة + +343 +00:24:57,090 --> 00:25:01,290 +مثلا ال API system هنشوف برضه نماذج مقتصرة أخرى + +344 +00:25:01,290 --> 00:25:02,470 +غير ال API system + +345 +00:25:05,080 --> 00:25:09,100 +احنا اتكلمنا على منتجات استخدمناها في التعرف على + +346 +00:25:09,100 --> 00:25:12,080 +ال microbenz تعرفنا انا اتكلم على منتجات اخرى سيئة + +347 +00:25:12,080 --> 00:25:16,660 +الذكر زي ال pyrogen ال pyrogen this is a fever + +348 +00:25:16,660 --> 00:25:21,020 +producing substance synthesized by بكتيري اش يعني + +349 +00:25:21,020 --> 00:25:25,840 +fever producing يعني لو دخل المركب ان فيريزمنا + +350 +00:25:25,840 --> 00:25:30,880 +بيصير انها حرارة مرتفعة حرارة مرتفعة خدوا بالك مش + +351 +00:25:30,880 --> 00:25:34,540 +ضرور البكتيري تكون جوه يكفي يكون ال pyrogen جوه + +352 +00:25:35,520 --> 00:25:40,160 +بتركزوا معايا في هذه المعلومة المهمة بتعرفوا ال + +353 +00:25:40,160 --> 00:25:43,660 +stringات؟ شفتوا ما هي ال stringات؟ بتكون أظن مسكرة + +354 +00:25:43,660 --> 00:25:49,580 +و معقبة، تمام؟ ال string هذه اللي تخيل إنها كانت + +355 +00:25:49,580 --> 00:25:54,320 +مغموسة ب إشريشيا كولاي و سكرناها و بعدين عقبناها + +356 +00:25:54,320 --> 00:25:57,580 +أو برنامج إشريشيا كولاي نأخد و نركب حالة نعرفها + +357 +00:25:57,580 --> 00:26:00,400 +Staphylococcus aureus تعرفولا Staphylococcus + +358 +00:26:00,400 --> 00:26:06,820 +aureus؟ لأن البكتيريا ماتت بعد ما عقبناهالكن لم + +359 +00:26:06,820 --> 00:26:10,420 +تختفي عن كوكب الأرض، لم تختفي من السرنجة، ضايق + +360 +00:26:10,420 --> 00:26:14,520 +المركبات منها، بقايا منها، زي و احنا لما .. لما + +361 +00:26:14,520 --> 00:26:19,420 +بنموت، نختفيش فجأة، بنتحلل تدريجيا ولا لا، نفس + +362 +00:26:19,420 --> 00:26:24,460 +الشيء الفكتيريا، ماتختفيش، تتحلل من ضمن المركبات + +363 +00:26:24,460 --> 00:26:27,460 +اللي بتتركها الفكتيريا، مركبات زي الـpeptidoglycan + +364 +00:26:27,460 --> 00:26:32,940 +زي الـtychoric acid، فاكرين الـtychoric acid؟ في + +365 +00:26:32,940 --> 00:26:36,420 +الـsports، dipicronic acidالـ lipovoisaccharide + +366 +00:26:36,420 --> 00:26:41,160 +مابتدمرش بسرعة أو بسهولة هذه المركبات وإن كان + +367 +00:26:41,160 --> 00:26:44,680 +البكتيريا ميتة لو استخدمنا سيرنج هذا حققنا مركب + +368 +00:26:44,680 --> 00:26:48,900 +معين مثلا antibiotic أو محلول في جسم الإنسان وداخل + +369 +00:26:48,900 --> 00:26:53,300 +جسم الإنسان حيصير عنده fever على الرغم من عدم وجود + +370 +00:26:53,300 --> 00:26:58,120 +بكتيريا وإنما مركبات اذا بعض المنتجات اللي بتعمل + +371 +00:26:58,120 --> 00:27:00,420 +fever بيسميها pyrogine + +372 +00:27:03,960 --> 00:27:07,420 +النظافة قبل التعقيم + +373 +00:27:13,950 --> 00:27:17,630 +لازم نطفو بالأول و بعدين نعقمه لإنه لابد أبدأ + +374 +00:27:17,630 --> 00:27:21,370 +ببيئة خالية أصلا من ال micro organism للأسف هذه + +375 +00:27:21,370 --> 00:27:26,190 +التقافة قد تكون غير موجودة عند المصنعين في مصالة + +376 +00:27:26,190 --> 00:27:29,650 +الأخضر، خاصة المحلية، برا لأ، برا في عندهم أنظمة + +377 +00:27:29,650 --> 00:27:33,750 +مراقبة بتخلي التقافة غصب عنهم موجودة، بس عندنا لأ، + +378 +00:27:33,750 --> 00:27:38,330 +أقولك ما لأ، هيمروا الغذاء بعملية طبخطبخ أو طهي + +379 +00:27:38,330 --> 00:27:42,470 +يعني حرارة مرتفعة يعني قطر للميكرو أورجانيزم أوك + +380 +00:27:42,470 --> 00:27:45,890 +انت قطرت للميكرو أورجانيزم لكن مركباتها ومنطلقاتها + +381 +00:27:45,890 --> 00:27:51,470 +ما زالت مولود بس هحب ندبها هنا Exotoxin و invasive + +382 +00:27:51,470 --> 00:27:58,010 +enzymes Exotoxins يعني سموم ابتنتجها البكتيريا + +383 +00:27:58,010 --> 00:28:00,450 +while alive عكس مين + +384 +00:28:08,150 --> 00:28:10,830 +الـ lipopolysaccharide اللي هو الـ lipid A اللي + +385 +00:28:10,830 --> 00:28:13,490 +موجود في الـ lipopolysaccharide في الـ outer + +386 +00:28:13,490 --> 00:28:16,250 +membrane تبع ال gram negative بكتيريا اعتقدوا ان + +387 +00:28:16,250 --> 00:28:19,470 +هذا مافيش اي خلاف كل gram negative بكتيريا تحتوي + +388 +00:28:19,470 --> 00:28:23,570 +على اندوتوكسين لكن بعض ال gram negative بكتيريا أو + +389 +00:28:23,570 --> 00:28:27,090 +بعض ال gram بصي بنتجوا ايضا اكزوتوكسين يعني + +390 +00:28:27,090 --> 00:28:30,250 +البكتيريا بتكون حية و بتنتج هذا التوكسين و اعطيكم + +391 +00:28:30,250 --> 00:28:35,280 +ميثار كتير سهل و بسيطبتعرفوا التيتانوس؟ حكينا عنه + +392 +00:28:35,280 --> 00:28:38,220 +مرة ال Clostridium Tetani أو Clostridium Tetani + +393 +00:28:38,220 --> 00:28:43,360 +لما بغز المصمار الشخص في قدمه على سبيل المثال ال + +394 +00:28:43,360 --> 00:28:47,480 +bacteria تدخل جوا ال spores و بتبدأ تتكاثر في بعض + +395 +00:28:47,480 --> 00:28:51,620 +الظروف معينة و بتنقسم في نفس المكان لا تغادر + +396 +00:28:51,620 --> 00:28:56,980 +المكان اللي دخلت منه و بتنتج ال toxin تبعها في نفس + +397 +00:28:56,980 --> 00:29:01,930 +المكانالتوكسين لأنه مُركّبات صغيرة و will diffuse + +398 +00:29:01,930 --> 00:29:05,510 +و في الآخر بيصل للدم و بروح لل central nervous + +399 +00:29:05,510 --> 00:29:09,630 +system البكتيريا الموجودة localized .. localized + +400 +00:29:09,630 --> 00:29:15,730 +يعني في مكان محدود بينما .. بينما المنتج تبعها + +401 +00:29:15,730 --> 00:29:19,350 +اللي هو ال exotoxin خرج منها و خرج من نطاق اللي + +402 +00:29:19,350 --> 00:29:21,630 +localized صار systemic و التأثير تبعه بيكون + +403 +00:29:21,630 --> 00:29:22,350 +systemic + +404 +00:29:32,510 --> 00:29:37,210 +مبتسم بشكل دائم، مبتسم بشكل دائم هو يعني هذا مؤشر + +405 +00:29:37,210 --> 00:29:40,950 +على أنه ليس كل ابتسامة هي تعبّل عن السعادة، ربما + +406 +00:29:40,950 --> 00:29:47,270 +يكون عنده Titanus، تمام؟فهذه نقطة كتير مهمة الان + +407 +00:29:47,270 --> 00:29:51,790 +كلمة invasive enzymes خلّيكم معايا انا فاندي خلية + +408 +00:29:51,790 --> 00:29:56,770 +و خلية مرتبطين في بعض في نسيج الان الارتباط هذا + +409 +00:29:56,770 --> 00:30:03,040 +بيجعل من الالأنسجة عائق أمام البكتيريا للاختراق، + +410 +00:30:03,040 --> 00:30:07,900 +لا تستطيع إنها تغزو مناطق بعيدة عن مكان تواجدها، + +411 +00:30:07,900 --> 00:30:12,060 +فاجتمع البكتيريا تنتج enzymes زي مثلا الكلاجينيز + +412 +00:30:12,060 --> 00:30:18,420 +والإلاستيز، الكلاجينيز والإلاستيز بيدوبوا + +413 +00:30:19,490 --> 00:30:23,410 +الفواصل ما بين الخلايا وبالتالي بيسمح للبكتيريا + +414 +00:30:23,410 --> 00:30:27,970 +انها تغزو الانسجار فمسموها invasive enzymes يعني + +415 +00:30:27,970 --> 00:30:32,090 +انزيمات تساعد البكتيريا في غزو الانسجار ووصل + +416 +00:30:32,090 --> 00:30:34,870 +الصورة ان فيها اندريجز توكسينز واخدنا مثلا لها + +417 +00:30:34,870 --> 00:30:38,550 +اللي استخدم تتانيه توكسين أو تتانوسبازمين واتكلمنا + +418 +00:30:38,550 --> 00:30:42,590 +عن invasive enzyme واخدنا مثل كولاجينيز and اللي + +419 +00:30:42,590 --> 00:30:48,180 +هو الاستيز نجي لل pigmentبعض المركبات البعض + +420 +00:30:48,180 --> 00:30:52,000 +البكتيريا ألوانها جميلة ألوانها جميلة تفيل + +421 +00:30:52,000 --> 00:30:55,480 +البكتيريا الخضرة هذه خضرة مزرقة أعتقد اللون يكون + +422 +00:30:55,480 --> 00:31:00,120 +أخضر مزرق بكتيريا اسمها Sodomonas aeruginosa + +423 +00:31:00,120 --> 00:31:04,820 +Sodomonas aeruginosa بتنتج pigment و اللي على + +424 +00:31:04,820 --> 00:31:08,020 +الجهة التانية اللون الأحمر هذه البكتيريا اسمها + +425 +00:31:08,020 --> 00:31:14,960 +Serratia marcesens لون أحمرالان بتتلاحظوا ملاحظة + +426 +00:31:14,960 --> 00:31:19,920 +نكية ان في اللون الأحمر اللون فقط موجود في + +427 +00:31:19,920 --> 00:31:24,740 +المستعمرات مش موجود في الطبق بينما في اللون + +428 +00:31:24,740 --> 00:31:30,650 +التانية منتشر في المستعمرات وموجود في وين؟فى الطبق + +429 +00:31:30,650 --> 00:31:35,390 +دلوقت عايش على ان water soluble و water insoluble + +430 +00:31:35,390 --> 00:31:40,050 +مين ال insoluble الحمرة اللى تأثيراتها مارس سنس + +431 +00:31:40,050 --> 00:31:46,290 +بينما السيدو موناس إيجينوزا water soluble هل هناك + +432 +00:31:46,290 --> 00:31:51,090 +فوائد لل pigment ولا مافيش فوائد البكيرة بتنتج بس + +433 +00:31:51,090 --> 00:31:56,090 +عشان تتلون يعنى تصبغ حاجة تشوفوا أحد الفوائد اللى + +434 +00:31:56,090 --> 00:31:59,010 +أخدنا أفهى عن السيدو موناس إيجينوزا أنها بتنتج في + +435 +00:31:59,010 --> 00:32:04,960 +الوسطتنتشر في الوسط وبترتبط في بعض المراكبات زي ال + +436 +00:32:04,960 --> 00:32:09,060 +iron بيصير ال iron هذا bound فاكرين ال bound + +437 +00:32:09,060 --> 00:32:12,880 +water؟ هل كان .. هل .. هل البكتيريا بتستفيد من ال + +438 +00:32:12,880 --> 00:32:17,520 +bound water؟ وإن ما بتستفيدش؟ الآن ال bound iron + +439 +00:32:17,520 --> 00:32:21,180 +اللي في الوسط هذا لو كان فيه بكتيريا أخرى في + +440 +00:32:21,180 --> 00:32:26,580 +المنطقة الآن لحظة هي مثلا في المنطقة هذه فيه + +441 +00:32:26,580 --> 00:32:30,420 +بكتيريا لكن هنا مافيش بكتيرياتخيلوا في استفيل + +442 +00:32:30,420 --> 00:32:33,640 +الكوكاس اوريس في المنطقة هذه، الاستفيل الكوكاس + +443 +00:32:33,640 --> 00:32:38,420 +اوريس بدها ايرون، فيه بدهم ايرون، لكن الايرون هذا + +444 +00:32:38,420 --> 00:32:42,840 +مرتبط، مرتبط مين؟ بالصبغة تبع ال .. ال .. ال .. ال + +445 +00:32:42,840 --> 00:32:43,340 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +446 +00:32:43,340 --> 00:32:43,500 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +447 +00:32:43,500 --> 00:32:44,460 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +448 +00:32:44,460 --> 00:32:45,000 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +449 +00:32:45,000 --> 00:32:47,180 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +450 +00:32:47,180 --> 00:32:47,200 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +451 +00:32:47,200 --> 00:32:47,360 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +452 +00:32:47,360 --> 00:32:47,360 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +453 +00:32:47,360 --> 00:32:51,810 +.. ال .. ال .. ال ..يعني بتجعل الحديد غير متعلمين + +454 +00:32:51,810 --> 00:32:56,970 +للآخرين، وسيلة من وسائل التنافس المخيفة، يعني + +455 +00:32:56,970 --> 00:33:01,390 +البشر مابيعرفوش يستخدم .. ينكروا هذا المقر أو .. + +456 +00:33:01,390 --> 00:33:04,230 +في زمان اتحادين بيعرفوا أكتر، بتعلموا من + +457 +00:33:04,230 --> 00:33:07,410 +البكتيريا، + +458 +00:33:07,410 --> 00:33:10,370 +يعني الآن زي واحد بيدي بالظبط مش بعمل .. بخش في + +459 +00:33:10,370 --> 00:33:14,160 +قاعة زي هذهوبروح بحط ورقة محجوز، محجوز، محجوز، + +460 +00:33:14,160 --> 00:33:17,620 +محجوز، تمام؟ كل اللي بيجوا بيلاقوا الورقة المحجوز + +461 +00:33:17,620 --> 00:33:21,220 +بيسحبوا عليهم إيه و بيطلعوا إلى الخارج، بيقدروا + +462 +00:33:21,220 --> 00:33:23,940 +يستفيدوا من الكراسي، معناه الكراسي موجودة، لكن أنا + +463 +00:33:23,940 --> 00:33:26,420 +وقت ما بدي باجي و بجد على كرسي، أي كرسي من الكراسي + +464 +00:33:26,420 --> 00:33:29,200 +هذا، فإذا البكتيريا وصلت لل iron، بتاخدوه و + +465 +00:33:29,200 --> 00:33:34,550 +بتستخدموه هناالبيجمنت في كل ميكروفيلة فائدة مختلفة + +466 +00:33:34,550 --> 00:33:39,530 +عن الآخر في بعض منها أساسي في الـ virulence، أساسي + +467 +00:33:39,530 --> 00:33:42,470 +في الـ virulence، يعني virulence فاكرة، المهم أنه + +468 +00:33:42,470 --> 00:33:47,010 +فيه pigments و فيه ألوان للبكتيرياسيداموناس + +469 +00:33:47,010 --> 00:33:50,570 +إيرجنوزا في ألوان أصفر وفي أخضر وفي أزرق وما + +470 +00:33:50,570 --> 00:33:56,810 +بينهما أصفر مخضر أخضر مصفر أزرق مخضر أخضر مصفر لكن + +471 +00:33:56,810 --> 00:33:59,910 +هاي ال pigments بتاعي هاي السيرات اللون الأحمر + +472 +00:33:59,910 --> 00:34:03,190 +القاني اللي بيشبه اللي حد كده بيسموه tomato juice + +473 +00:34:03,190 --> 00:34:07,690 +يعني عصير الطماطم اللون بتاعها إذا في عندي مجموعة + +474 +00:34:07,690 --> 00:34:11,750 +من المنتجات اللي بتنتجها الوقت إكسدوكسين invasive + +475 +00:34:11,750 --> 00:34:13,950 +enzymes وهي كان عنها pigments + +476 +00:34:16,250 --> 00:34:20,290 +انتيبايتكس تنتج البكتيريا هو ال .. ال .. ال .. ال + +477 +00:34:20,290 --> 00:34:21,930 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +478 +00:34:21,930 --> 00:34:21,950 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +479 +00:34:21,950 --> 00:34:23,450 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +480 +00:34:23,450 --> 00:34:23,730 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +481 +00:34:23,730 --> 00:34:24,110 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +482 +00:34:24,110 --> 00:34:33,010 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +483 +00:34:33,010 --> 00:34:33,650 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +484 +00:34:33,650 --> 00:34:33,690 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +485 +00:34:33,690 --> 00:34:33,750 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +486 +00:34:33,750 --> 00:34:33,850 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +487 +00:34:33,850 --> 00:34:33,850 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +488 +00:34:33,850 --> 00:34:33,970 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +489 +00:34:34,020 --> 00:34:37,780 +البنسينيين من فطر يعني اقصدوا ان البكتيريا .. + +490 +00:34:37,780 --> 00:34:40,060 +البكتيريا بتنتج .. أو بعض البكتيريا .. ال + +491 +00:34:40,060 --> 00:34:44,100 +actinomycetes أشهر مجموعة حكينا عنها مبرح .. لما + +492 +00:34:44,100 --> 00:34:47,920 +اتكلمنا عن المطر، تمام؟ و ان الرايحة اللي بنشتمها + +493 +00:34:47,920 --> 00:34:51,600 +أولة تشت الدنيا ناتجة عن ال actinomycetes، ال + +494 +00:34:51,600 --> 00:34:54,860 +actinomycetes من أشهر منتجات ال antibiotic في + +495 +00:34:54,860 --> 00:35:01,140 +الدنيا، تمام؟Bacteriocenes، هناك بعض المركبات لا + +496 +00:35:01,140 --> 00:35:05,540 +ترتقي نسميها Antibiotics، لكن نقدر نوصفها + +497 +00:35:05,540 --> 00:35:09,540 +Antibiotic-like substances، يعني مركبات تشبه الـ + +498 +00:35:09,540 --> 00:35:11,780 +Antibiotic، إيش يعني ممكن تشبهها؟ تشبهها + +499 +00:35:11,780 --> 00:35:17,440 +بالactions، ابتنتجها البكتيريا في أمعاقنا، حتى تحد + +500 +00:35:17,440 --> 00:35:21,630 +من نمو الآخرإحنا لو .. لو اتكلمنا عن البكتيريا + +501 +00:35:21,630 --> 00:35:25,730 +اللى موجودة في معايا الإنسان هي عبارة عن دوار أغمى + +502 +00:35:25,730 --> 00:35:30,270 +.. دوار أغمى تتنافس فيما بينها بأسلحة متنوعة + +503 +00:35:30,270 --> 00:35:34,150 +ومختلفة فعشانك فيه اتزال لإن كل واحد عارف قوة + +504 +00:35:34,150 --> 00:35:37,830 +الآخر .. عارف قوة الآخر من ضمن الأسلحة المستخدمة + +505 +00:35:37,830 --> 00:35:41,770 +في التوازن هو ال bacteriocenes اللي هنا بتشبه مين + +506 +00:35:41,770 --> 00:35:45,470 +ال antibiotics و هناك بعض البكتيريا بتنتج vitamins + +507 +00:35:45,470 --> 00:35:48,860 +و احنا قولنا لكم كل أحد فوائدال normal flora في + +508 +00:35:48,860 --> 00:35:52,060 +الأمعاق أو ال normal microbiota هو إنتاج بعض أنواع + +509 +00:35:52,060 --> 00:35:57,080 +من اللي هو ال vitamin z و ال vitamin k يبقى فيه + +510 +00:35:57,080 --> 00:36:02,700 +منتجات ضارة فيه منتجات جيدة فيه virulence factors + +511 +00:36:02,700 --> 00:36:06,040 +فيه toxins فيه أشياء مستغللة احنا في التعرف على ال + +512 +00:36:06,040 --> 00:36:11,540 +micro organisms الآن + +513 +00:36:11,540 --> 00:36:15,610 +نيجي لاستغلال كل هذه المعلومات اللي دراسناها من + +514 +00:36:15,610 --> 00:36:21,230 +أول المحاضرة اليوم ومبارح إلى توظيف هذه المعلومات + +515 +00:36:21,230 --> 00:36:26,850 +في المعمل artificial cultivation of bacteria يعني + +516 +00:36:26,850 --> 00:36:30,050 +الآن بدنا نمي البكتيريا بشكل صناعي طب ما هي بتنمو + +517 +00:36:30,050 --> 00:36:33,550 +في ال .. في البيئة و بتتكاتر في البيئة لكن انا بدي + +518 +00:36:33,550 --> 00:36:37,390 +نميها بشكل صناعي طبعا تنميت ان البكتيريا أكيد ليست + +519 +00:36:37,390 --> 00:36:43,430 +رفاهية وانما لغرض او لمجموعة أغراضمن مجموعة + +520 +00:36:43,430 --> 00:36:46,830 +الأغراض هذه أن نتعرف على البكتيريا لا أستطيع أن + +521 +00:36:46,830 --> 00:36:50,350 +أتعرف على البكتيريا بشكل دقيق بيسموها ال golden + +522 +00:36:50,350 --> 00:36:55,310 +standard يعني أفضل وسيلة للتعرف على البكتيريا هو + +523 +00:36:55,310 --> 00:37:00,510 +تنميتها in pure culture بمزرعة نقية ومن ثم دراسة + +524 +00:37:00,510 --> 00:37:03,570 +خصائصها ايه كانت دراسة خصائصها gram positive ولا + +525 +00:37:03,570 --> 00:37:06,050 +gram negative اندولبوسطف ولا اندولبوسطف اللي هو ال + +526 +00:37:06,050 --> 00:37:10,710 +biochemical test اللي هتعلموهم في المعمليُطلَق على + +527 +00:37:10,710 --> 00:37:15,390 +الوسط المُستخدم في تنمية البكتيريا Medium و جمعها + +528 +00:37:15,390 --> 00:37:20,630 +Medium أووساط Media Medium وسط It's a liquid or + +529 +00:37:20,630 --> 00:37:25,490 +solid mixture خلطة سائلة أو صلبة designed to + +530 +00:37:25,490 --> 00:37:30,090 +support the growth of microorganisms usually + +531 +00:37:30,090 --> 00:37:33,590 +called broth of liquid إذا كانت سائر بنسميها broth + +532 +00:37:33,590 --> 00:37:38,720 +و broth معناها ملق شورب يعني تمام؟و usually called + +533 +00:37:38,720 --> 00:37:42,880 +agar if solid بنسميها agar لإن اصلا نضيف مادة + +534 +00:37:42,880 --> 00:37:46,980 +اسمها agar agar تمام بتخليها بالظبط solid إذن في + +535 +00:37:46,980 --> 00:37:51,480 +عندي شكلين أساسيين في الوسط الذراعية liquid and + +536 +00:37:57,010 --> 00:38:02,110 +اللي هو يعني بنضيف كمية أجار، بالضبط، حتى يجعل + +537 +00:38:02,110 --> 00:38:07,290 +قوامه يعني semi-solid، مش solid و لا هو liquid لغة + +538 +00:38:07,290 --> 00:38:11,110 +بواحد، وهو حقيقة فحص ال motility، فحص ال motility، + +539 +00:38:11,110 --> 00:38:16,920 +هالكلة وانا لوحدي فعلا، طيبالأوساط الزراعية هناك + +540 +00:38:16,920 --> 00:38:20,280 +تصنيفات إلا بشكل كبير يعني .. يعني .. يعني .. خدنا + +541 +00:38:20,280 --> 00:38:24,580 +نقول نجا نصنيفها بأكتر من وسيلة من ضمن الوسائل + +542 +00:38:24,580 --> 00:38:28,580 +according to basic ingredients المحتوى ال .. ال .. + +543 +00:38:28,580 --> 00:38:31,260 +ال .. ال .. أو المحتويات اللي موجودة في الوساطة + +544 +00:38:31,260 --> 00:38:34,880 +فمثلا عندنا حاجة Minimal Essential Growth Medium + +545 +00:38:34,880 --> 00:38:38,860 +ومن اسمه Minimal Essential Growth Medium + +546 +00:38:43,820 --> 00:38:48,340 +بمعنى أخر، الحد الأدنى من المطلوبات للنمو، يعني + +547 +00:38:48,340 --> 00:38:51,620 +ينتلق هذا الوسط، الحد الأدنى من المطلوبات للنمو، + +548 +00:38:51,620 --> 00:38:57,740 +مش ال enrichment main يعني، + +549 +00:38:57,740 --> 00:39:03,660 +بده شرح هذا، نركز مع بعض، فاكرين لما اتكلمنا عن + +550 +00:39:03,660 --> 00:39:07,280 +البكتيريا وسط ال wall، كنا البكتيريا اللى بتنزل في + +551 +00:39:07,280 --> 00:39:13,110 +الحمام، بتنزل في الماء الحلوة، أمام؟و الميه العذبة + +552 +00:39:13,110 --> 00:39:17,690 +مافيش فيها ملح بكميات كافية تعمل balance بين ال .. + +553 +00:39:17,690 --> 00:39:20,390 +بالضغط الاثموذي اللي تبقى على الخلية و ال .. و + +554 +00:39:20,390 --> 00:39:24,090 +البيئة وبالتالي بيكون البكتيريا under stress وفي + +555 +00:39:24,090 --> 00:39:28,230 +البيئة البكتيريا معظم الأوقات بتكون under stress + +556 +00:39:28,230 --> 00:39:32,110 +ومدام under stress لما انا ابتدأ .. اقولكوا بلاش + +557 +00:39:32,110 --> 00:39:34,310 +في رسم الإنسان .. لما انا ابتدأ أقل من رسم الإنسان + +558 +00:39:34,310 --> 00:39:37,310 +مش في حرب دائرة أساسا احنا بين .. بين البكتيريا و + +559 +00:39:37,310 --> 00:39:40,190 +بين ال immune system فممكن البكتيريا تكون under + +560 +00:39:40,190 --> 00:39:40,830 +stress + +561 +00:39:43,680 --> 00:39:47,680 +طبعا باللغة العربية هتكون كلمة مجروحة لكن مقصود + +562 +00:39:47,680 --> 00:39:53,780 +فيها أنه مش في صحتها يعني مش بأفضل صورها عشان أنا + +563 +00:39:53,780 --> 00:40:00,720 +أنميها لازم أحطها في وسط غني جدا و يساعدها على + +564 +00:40:00,720 --> 00:40:06,220 +الاستجمام من المصائب اللي ألمت فيها و من ال stress + +565 +00:40:06,220 --> 00:40:09,260 +اللي كان اتوقعله و هذا الوسط غالبا بيسميه احنا ايه + +566 +00:40:09,260 --> 00:40:14,240 +enrichment mainlyفي المستقبل هنعيد تعريفه بما + +567 +00:40:14,240 --> 00:40:17,920 +يتلقى مع موضوعات أخرى لكن هذا لأن الحد الأدنى اللي + +568 +00:40:17,920 --> 00:40:23,040 +هنفهمه أنها أوساط غنية جدا تستخدم لإنعاش بعض + +569 +00:40:23,040 --> 00:40:29,260 +الميكروبات اللي هي بتكون under stress نيجي للجزء + +570 +00:40:29,260 --> 00:40:32,180 +أو النوع التالت اللي هو ال selective medium + +571 +00:40:35,200 --> 00:40:41,000 +عبارة عن مصدر استخدمناه لانتقاء نوع معين من + +572 +00:40:41,000 --> 00:40:46,160 +البكيري كيف ان انتقاء نوع معين؟ تخيلوا لو انا بدى + +573 +00:40:46,160 --> 00:40:51,380 +احكم على غذاء انه صالح للاستخدام او غير صالح + +574 +00:40:51,380 --> 00:40:55,800 +للاستخدام والمواصفة الفلسطينية للأغذية او لهذا + +575 +00:40:55,800 --> 00:40:59,920 +الغذاء بتقول يجب ان يكون هناك اكثر من مئة + +576 +00:40:59,920 --> 00:41:06,090 +staphylococcus aureusطيب يبقى أنا الآن مضطر أعد + +577 +00:41:06,090 --> 00:41:10,810 +فقط مين؟ طب هو يمكن يكون ما يكون بعد أخرى مسموح + +578 +00:41:10,810 --> 00:41:14,770 +فيها، أنا مابديها، المواصفة بتقول اعد اللي جداش + +579 +00:41:14,770 --> 00:41:22,330 +فيه استفيد الكوكاس or yes فلازم أصمم مواصف لا ينمي + +580 +00:41:22,330 --> 00:41:24,650 +إلا باستفيد الكوكاس or yes + +581 +00:41:28,120 --> 00:41:32,820 +يعني باقتصاد كله لأننا عارفه بنقول هو وسط يستخدم + +582 +00:41:32,820 --> 00:41:38,780 +للنمو والانتقاء للبكتيريا نجلة الأخيرة اللي هو ال + +583 +00:41:38,780 --> 00:41:42,220 +differential أعطاني مره معناها مصطلح differential + +584 +00:41:42,220 --> 00:41:47,360 +ال differential معناها تميز يميز صح ال + +585 +00:41:47,360 --> 00:41:52,240 +differential media هي وسط ت differentiate between + +586 +00:41:52,240 --> 00:41:54,940 +two similar organisms او بتعطيكم مثال + +587 +00:42:03,350 --> 00:42:07,390 +السلمونيلا والإيشريشيا كولاي التنتين انترو + +588 +00:42:07,390 --> 00:42:11,230 +بكترياسي التنتين انترو بكترياسي والتنتين مكون + +589 +00:42:11,230 --> 00:42:13,990 +موجودين في ال intestine يعني موجودين في عينة ال + +590 +00:42:13,990 --> 00:42:17,830 +stool عينة الكولا إذا أكرمكم الله إذا بدي أعزل + +591 +00:42:17,830 --> 00:42:21,630 +السلمونيلا من الوسط بروح بحطها في وسط اسم الماكنكي + +592 +00:42:21,630 --> 00:42:26,670 +الماكنكي بيميزلي بين two types of microorganisms + +593 +00:42:26,670 --> 00:42:32,020 +بين lactose fermenter و لاكتوز فرمنترواحدة أعطاها + +594 +00:42:32,020 --> 00:42:36,440 +لون أحمر وواحدة ماعطاهاش لون خالص دي في وسط عمل + +595 +00:42:36,440 --> 00:42:41,580 +differentiation عمل differentiation فدي بقى لكوا + +596 +00:42:41,580 --> 00:42:48,300 +رقم Z وD من أعظم الإنجازات في مختبرات ال + +597 +00:42:48,300 --> 00:42:53,040 +microbiology ومن كتر ما هم مهمين غالبا بيلاجيش + +598 +00:42:53,040 --> 00:42:56,460 +selective لحاله في ال medical و differential لحاله + +599 +00:42:56,460 --> 00:43:00,870 +فبيلاجي الوسط نفسه بيمتلك ايه الصفتانبسميه + +600 +00:43:00,870 --> 00:43:05,190 +selective differential نرجع للمقنكي المقنكي + +601 +00:43:05,190 --> 00:43:09,630 +selective بحيث انه وضع فيه بعض المركبات بحيث لا + +602 +00:43:09,630 --> 00:43:12,070 +ينمي ال gram booster فهو selective ده ال gram + +603 +00:43:12,070 --> 00:43:16,270 +negative وإذا استطيع ان يميز بين lactose fermenter + +604 +00:43:16,270 --> 00:43:18,710 +و لا lactose fermenter فهو differential + +605 +00:43:23,950 --> 00:43:26,690 +كمان الوضع ده اللي استخدمناه لعدل Staphylococcus + +606 +00:43:26,690 --> 00:43:30,070 +aureus سميناه selective صح؟ برضه differential لأنه + +607 +00:43:30,070 --> 00:43:34,350 +للأسف خلناه selective لل Staphylococci بس مش لل + +608 +00:43:34,350 --> 00:43:37,610 +Staphylococcus aureus فعشان نعدل Staphylococcus + +609 +00:43:37,610 --> 00:43:41,370 +aureus خلناه differential Staphylococcus aureus + +610 +00:43:41,370 --> 00:43:46,290 +بتبين صفرة وما قعداها ببين حمر أحمر فصار selective + +611 +00:43:46,290 --> 00:43:51,850 +andأعتقد ان هناك الصورة بتكون واضحة تماما ان انا + +612 +00:43:51,850 --> 00:43:55,410 +استطيع أن أصمم أوصات زراعية في ال microbiology و + +613 +00:43:55,410 --> 00:43:59,650 +انا بقولك كده مرة هذا التصميم يجب ان لا يقتصر على + +614 +00:43:59,650 --> 00:44:03,170 +من صممه كمان انتوا عندكوا المقدر على التفكير و + +615 +00:44:03,170 --> 00:44:06,070 +عندكوا المقدر على انكوا لو طلبت منا في يوم من + +616 +00:44:06,070 --> 00:44:09,870 +الأيام ان نعمل modification او ممكن نفكر احنا في + +617 +00:44:09,870 --> 00:44:15,120 +modification او نخترع أوصات جديدةفي مقدرتنا و بس + +618 +00:44:15,120 --> 00:44:19,560 +بدنا نقضي وقت أطول في دراسة المعلومات المتعلقة + +619 +00:44:19,560 --> 00:44:23,780 +ببعض ال micro organism حتى نستطيع نوفر لها الصفات + +620 +00:44:23,780 --> 00:44:26,140 +المناسبة نكمل المرة الجاي ان شاء الله كل العالم و + +621 +00:44:26,140 --> 00:44:26,800 +أنتم بخير + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/UgPzLIzLs8w_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/UgPzLIzLs8w_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..439be03982decf04b2a5b869a45c58a416e42341 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/UgPzLIzLs8w_raw.srt @@ -0,0 +1,2228 @@ +1 +00:00:20,540 --> 00:00:22,360 +أعوض بالله السلام علي المشي طالع جيد بسم الله + +2 +00:00:22,360 --> 00:00:26,260 +الرحمن الرحيم نكمل ال strip to cocci توقفنا المرة + +3 +00:00:26,260 --> 00:00:29,400 +الماضية عند نوع آخر من ال classification ل strip + +4 +00:00:29,400 --> 00:00:33,460 +to cocci اللي هو Lansfield grouping أو Lansfield + +5 +00:00:33,460 --> 00:00:37,800 +classification وعرفنا أنه Rebecca Lansfield + +6 +00:00:37,800 --> 00:00:40,920 +classified strip to cocci based on a surface + +7 +00:00:40,920 --> 00:00:45,640 +antigen into groups كلمة group تعني أن one or more + +8 +00:00:45,640 --> 00:00:51,230 +species are included in that groupكان مرة based on + +9 +00:00:51,230 --> 00:00:55,530 +surface antigen للأسف لجينا أن هذا ال surface + +10 +00:00:55,530 --> 00:00:59,810 +antigen موجود في بعض الstriptococci وبتالي صنفناها + +11 +00:00:59,810 --> 00:01:03,190 +وسميناها groupable يعني قابلة للتصنيف أو قابلة + +12 +00:01:03,190 --> 00:01:08,330 +للوضع في مجموعات مثل مجموعة A وB وD وهي الأهم على + +13 +00:01:08,330 --> 00:01:12,130 +الإطلاق للstriptococci هناك مجموعات أقل أهمية ليها + +14 +00:01:12,130 --> 00:01:16,270 +أهمية لكن أقل زي الـC وG وF وهي نادرة أن تسبب + +15 +00:01:16,270 --> 00:01:20,940 +أمراض للإنسانو ال non-groovable اللي هما كانوا + +16 +00:01:20,940 --> 00:01:25,140 +مثلين stirtococcus pneumonia و طبعا هي مشهورة كتير + +17 +00:01:25,140 --> 00:01:27,800 +يعني مش بقى احكي انها non-groovable لأنها اقل + +18 +00:01:27,800 --> 00:01:31,770 +اهمية لأ الاهامية كبيرةال viridans streptococci + +19 +00:01:31,770 --> 00:01:35,190 +اللي بتمثلها ال streptococcus mutants اللي بتعملها + +20 +00:01:35,190 --> 00:01:39,450 +dental كارث و مجموعة أخرى من أفرادها سميناها + +21 +00:01:39,450 --> 00:01:42,810 +viridans group ده بتتذكروا viridans group مجموعة + +22 +00:01:42,810 --> 00:01:47,850 +الخضراء اللي قد أو خدنا نقول في evidence عالي جدا + +23 +00:01:47,850 --> 00:01:52,390 +من الأبحاث بتقول أنها ممكن تسبب endocarditis + +24 +00:01:52,390 --> 00:01:56,710 +endocarditis تمتلك مقدرة مهولة على ال attachment + +25 +00:01:57,160 --> 00:02:00,660 +فبالتالي عندها المقدرة في القلب، تشوف القلب نفسه، + +26 +00:02:00,660 --> 00:02:04,320 +عدد القلب تبقى هناك، وين في القلب؟ مضخة الدم، يعني + +27 +00:02:04,320 --> 00:02:08,400 +في قوة شديدة من الضغط قد يدفع البكتيريا إلى + +28 +00:02:08,400 --> 00:02:11,860 +الخارج، لكن مقدرتها ال attachment عالية كمان كده + +29 +00:02:11,860 --> 00:02:15,480 +زي + +30 +00:02:15,480 --> 00:02:18,220 +أي بكتيريا أخرى في عهد الجينك تمتلك toxins و + +31 +00:02:18,220 --> 00:02:22,340 +enzymes، لكن نعود بكم إلى التصنيف التاني أو + +32 +00:02:22,340 --> 00:02:26,200 +الأولاني، الأساسي اللي احنا أخدناه، اللي هو القلنا + +33 +00:02:26,200 --> 00:02:30,240 +إن الـ Structococci قد تُصنّف إما Alpha أو Beta أو + +34 +00:02:30,240 --> 00:02:33,060 +Gamma والـ Alpha اللي هي ال incomplete .. + +35 +00:02:33,060 --> 00:02:37,140 +incomplete partial hemolysis as indicated by + +36 +00:02:37,140 --> 00:02:40,280 +greenish discoloration يعني التلون باللون الأخضر + +37 +00:02:40,280 --> 00:02:44,730 +حول المستعمرالـ Beta-Hemolysis complete as + +38 +00:02:44,730 --> 00:02:47,710 +indicated by complete clearance يعني مافيش أي لون + +39 +00:02:47,710 --> 00:02:51,230 +حول المستعمر الجامعة زي ما ذكرنا جاهزة ترضية + +40 +00:02:51,230 --> 00:02:54,930 +عطناها للبكتيريا non-hemolytic اللي لم تعملش + +41 +00:02:54,930 --> 00:02:58,930 +Hemolysis هذا الجدول مش لدى الحفظ الجدول هذا فقط + +42 +00:02:58,930 --> 00:03:02,190 +عشان يعطينا إيش ورا إن هناك different species + +43 +00:03:02,190 --> 00:03:06,490 +under different groups ال groups اللي هندرسها هي + +44 +00:03:06,490 --> 00:03:11,400 +اللي هنحفظها أما مش الجدول هذاالصورة اللى قدامنا + +45 +00:03:11,400 --> 00:03:17,260 +هذى صورة كتير معبرة على الجهة اليسرة من اللوحة + +46 +00:03:17,260 --> 00:03:21,920 +مبطلة بنلاقي أسماء كثيرة لأمراض مثلا meningitis, + +47 +00:03:23,100 --> 00:03:26,280 +otitis, sinusitis, tonsillitis, anpharyngitis + +48 +00:03:26,280 --> 00:03:31,880 +عندنا adenitis, pneumonia, endocarditis, impetigo, + +49 +00:03:32,020 --> 00:03:35,620 +ersepilas, impetigo مرمعانة جابت هناك؟ اه بتنتقل + +50 +00:03:35,620 --> 00:03:40,230 +.. وين مرمعانة؟ philostepin O, coccus, aureusهنا + +51 +00:03:40,230 --> 00:03:44,870 +قلنا نجا فى لحظة و نتكلم عن مفهوم مولود فى ال + +52 +00:03:44,870 --> 00:03:49,870 +microbiology two different organisms causes one + +53 +00:03:49,870 --> 00:03:53,630 +disease هى MBT جوه و هناك MBT جوه نفس المسمى نفس + +54 +00:03:53,630 --> 00:03:57,050 +الأعراض طبعا لكن لما نيجى نتكلم عن ال treatment + +55 +00:03:57,050 --> 00:04:01,850 +لمرض واحد قد تسببه أكتر من ال microbe ايه بتكون + +56 +00:04:01,850 --> 00:04:06,360 +الحل عندنا؟إنه لازم نعرف الميكروب المسبب للمرض حتى + +57 +00:04:06,360 --> 00:04:09,740 +نعطي specific treatment الان هذا أنا جلبت مثال + +58 +00:04:09,740 --> 00:04:14,120 +بسيط على two organism two different organism cause + +59 +00:04:14,120 --> 00:04:18,160 +one similar disease لكن بجريت مثال متطرف أكثر من + +60 +00:04:18,160 --> 00:04:24,880 +هذا المثال meningitis خلال دراستنا السابقة و + +61 +00:04:24,880 --> 00:04:29,000 +اللاحقة معظم الميكروبات اللي احنا ندرسها ممكن + +62 +00:04:29,000 --> 00:04:31,520 +يتسبب meningitis إذا ال meningitis + +63 +00:04:34,540 --> 00:04:38,960 +types of bacteria طيب لو قلنا ان it is caused by + +64 +00:04:38,960 --> 00:04:42,740 +bacteria it is caused by parasites مش بكتيريا + +65 +00:04:42,740 --> 00:04:46,940 +طفيليات بروتازوفا it may be caused by fungites + +66 +00:04:46,940 --> 00:04:50,640 +كريبتوكوكس نيوفورمس مشهور جدا الخميرة هذه بسبب + +67 +00:04:50,640 --> 00:04:54,620 +meningitis viruses viral meningitis تسمع بال viral + +68 +00:04:54,620 --> 00:04:58,500 +meningitis؟ viral meningitis يبقى فيروسات وبكتيريا + +69 +00:04:58,500 --> 00:05:01,260 +وطفيليات وفطريات + +70 +00:05:02,920 --> 00:05:07,200 +يعني الميكروبيولوجي كله بيعمل meningitis طيب لما + +71 +00:05:07,200 --> 00:05:11,780 +نشخص meningitis هذه خطوة ان المريض has a + +72 +00:05:11,780 --> 00:05:15,880 +meningitis disease لكن مين المسبب؟ هل هو viral؟ هل + +73 +00:05:15,880 --> 00:05:18,200 +هو bacterial؟ وإيه ده bacterial؟ من نوع ال + +74 +00:05:18,200 --> 00:05:21,960 +bacteria بالظبط؟ عشان نعطي specific treatment وهنا + +75 +00:05:21,960 --> 00:05:25,620 +الدور العظيم اللي انت بتقوم فيه كميكروبيولوجيين في + +76 +00:05:25,620 --> 00:05:29,140 +المختبرات انكوا تحاولوا تعزله العامل المسبب + +77 +00:05:29,140 --> 00:05:34,560 +ويتعرفوا عليه وأيضا كمانتشوفوا مدى حساسيته أو + +78 +00:05:34,560 --> 00:05:37,480 +مقاومته للمضاد الحيوي، إذا الدور اللي مانطبكم بهذه + +79 +00:05:37,480 --> 00:05:40,600 +الحلقة هو دور بالفعل يعني طيب بيقول meningitis أو + +80 +00:05:40,600 --> 00:05:44,680 +suspected meningitis، انتم تتعرفوا على المسبب لـ + +81 +00:05:44,680 --> 00:05:48,840 +meningitis وقد تستطيعوا أنكوا تعطوا الطبيب معلومات + +82 +00:05:48,840 --> 00:05:56,620 +أخرى مثل مدى حساسيتها للمضاد الحيوي أو مقاومتهإذن + +83 +00:05:56,620 --> 00:06:01,480 +في عندي أنا قائمة طويلة على اسم الإنسان من الأمراض + +84 +00:06:01,480 --> 00:06:04,140 +بتسببها الـ streptococcus المرة الماضية أنا + +85 +00:06:04,140 --> 00:06:06,380 +قلتلكوا معلومة، أنا قلتلكوا الـ staphylococcus + +86 +00:06:06,380 --> 00:06:10,420 +aureus هي أكثر مايكروب هتعزله في ال clinical + +87 +00:06:10,420 --> 00:06:14,120 +microbi من ال gram-boston، مش من كل البكتيريا، من + +88 +00:06:14,120 --> 00:06:17,830 +ال gram-bostonلكن اليوم أنا بقولكوا إن الـ + +89 +00:06:17,830 --> 00:06:20,350 +streptococcus و هذا الكلام برضه حكيته المرة + +90 +00:06:20,350 --> 00:06:24,890 +الماضية بأكد عليه إنه بتسبب أكتر أنواع من العدوى + +91 +00:06:24,890 --> 00:06:31,190 +أكتر أنواع و ليس more frequent بختلف المصطلحين هذه + +92 +00:06:31,190 --> 00:06:34,790 +أكتر إحداثة من العدوى هذه أكتر إحداثة لأنواع + +93 +00:06:34,790 --> 00:06:39,250 +مختلفة من العدوى كما نشاهد على اللوحة اللي أمامنا + +94 +00:06:39,250 --> 00:06:43,550 +group A streptococcal infection + +95 +00:06:46,300 --> 00:06:49,220 +Group A، وبعدين أنا اتكلم مع مين أنا؟ اتكلم على + +96 +00:06:49,220 --> 00:06:54,260 +نوع محدد من ال strip to focus وقبل ما ابدأ فيه + +97 +00:06:54,260 --> 00:06:59,610 +الصحيح لازم انا احاول اشبك بين معلومتانالمعلومة + +98 +00:06:59,610 --> 00:07:02,810 +الأولى في التصنيف قلنا Alpha, Beta, Gamma صح؟ + +99 +00:07:02,810 --> 00:07:05,890 +المعلومة التانية Group A, Group B, Group C ليها + +100 +00:07:05,890 --> 00:07:08,870 +Serological Classification أو Lansfield + +101 +00:07:08,870 --> 00:07:11,650 +Classification كلمة Serology يعني Antibody + +102 +00:07:11,650 --> 00:07:15,770 +وAntigen واحنا قلنا أنه Lansfield صنفت الميكروبات + +103 +00:07:15,770 --> 00:07:20,050 +هذه Based on Surface Antigen يبجي هو Serology أو + +104 +00:07:20,050 --> 00:07:23,570 +Serological Classification الآن Group A فيها + +105 +00:07:23,570 --> 00:07:27,980 +مجموعة من الميكروبات من الأسماءالوحيد اللي فيهم + +106 +00:07:27,980 --> 00:07:31,040 +الـ Beta-Hemolytic هي الـ Streptococcus Biogenes، + +107 +00:07:31,040 --> 00:07:34,360 +فماشي جاروا الألماء و الأطباء، جاروا احنا طيب لو + +108 +00:07:34,360 --> 00:07:37,840 +نمجنا two systems مع بعض و جورنا group A Beta + +109 +00:07:37,840 --> 00:07:41,700 +-Hemolyticاحنا واقع الأمر حددنا هوية الكائن اللي + +110 +00:07:41,700 --> 00:07:45,840 +بدناه يعني اللي هو مين? streptococcus bigenes إذا + +111 +00:07:45,840 --> 00:07:50,160 +للأقل لو سمعتوا مصطلح بيقول group A beta hemolytic + +112 +00:07:50,160 --> 00:07:53,980 +streptococci احنا بنقصد مين ل streptococcus + +113 +00:07:53,980 --> 00:07:58,560 +bigenes و لحد الآن كتير كتب و كتير مراجعة will + +114 +00:07:58,560 --> 00:08:01,840 +refer to this organism as group A streptococci حتى + +115 +00:08:01,840 --> 00:08:04,700 +بدون ما يقولوا beta hemolytic لأنها انشهرت بهذا + +116 +00:08:04,700 --> 00:08:12,900 +الاسمبتصيب البشر في كل الأعمار لكن أكتر فئة عمرية + +117 +00:08:12,900 --> 00:08:16,520 +مستهدفة من هذه البكتيريا هي الفئة العمرية من خمسة + +118 +00:08:16,520 --> 00:08:21,820 +لخمس عشر سنة اتكلمنا عن group A streptococci و + +119 +00:08:21,820 --> 00:08:25,100 +اللي هي beta hemolytic و اتكلمنا عن streptococcus + +120 +00:08:25,100 --> 00:08:28,120 +pyrogens تذكروا دائما ان الموضوع اللي بتتكلم فيه + +121 +00:08:28,120 --> 00:08:31,540 +هو البكتيريا المقيحة الأصطديدية اللي بتنتج + +122 +00:08:31,540 --> 00:08:36,800 +infection with PASممكن تعمل non-invasive diseases + +123 +00:08:36,800 --> 00:08:40,780 +زي الفرنجايتس وكلمة فرنجايتس التهاب الحلق والتهاب + +124 +00:08:40,780 --> 00:08:46,220 +الحلق بيشمله اللي هو التونسولايتس skin infections + +125 +00:08:46,220 --> 00:08:49,100 +لما بقول skin infections بيكونوا يتذكروا أن ال + +126 +00:08:49,100 --> 00:08:52,360 +skin هو أكبر organ في رسم الإنسان وبالتالي احتمال + +127 +00:08:52,360 --> 00:08:55,880 +الإصابة من الرأس إلى أخمص القدم موجودة ال impetigo + +128 +00:08:55,880 --> 00:08:57,920 +حكينا عنها اللي هي الحصف اللي يقول إنها مشترك مع + +129 +00:08:57,920 --> 00:09:01,920 +ال staphylococcus aureusفينا invasive bacterium + +130 +00:09:01,920 --> 00:09:04,720 +toxic shock like syndrome اعتقد ان احنا فهمنا ال + +131 +00:09:04,720 --> 00:09:10,120 +toxic shock syndrome و امبارح انا نزلتلكوا على ال + +132 +00:09:10,120 --> 00:09:12,240 +.. ال .. ال essential medical microbiology group + +133 +00:09:12,240 --> 00:09:15,540 +flash على ال super energy and how .. how they work + +134 +00:09:15,540 --> 00:09:20,000 +ارجو من حضارتكم انه تقضوا 3 دقائق فقط لن تنسوا ال + +135 +00:09:20,000 --> 00:09:22,620 +mechanism of action ابدا إذا رأيتم ال flash + +136 +00:09:22,620 --> 00:09:26,130 +animationوكان فيه flash animation اخر اخر عن ال + +137 +00:09:26,130 --> 00:09:29,410 +phagocytosis برضه كيف ال macro phage يبتعمل + +138 +00:09:29,410 --> 00:09:32,650 +phagocytosis وكيف يبتعمل processing لل antigen + +139 +00:09:32,650 --> 00:09:35,570 +وكيف يبتعمل ال antigen presentation فيا تفرجوا + +140 +00:09:35,570 --> 00:09:39,410 +عليها flash eating bacteria الصورة هذه اللي + +141 +00:09:39,410 --> 00:09:42,790 +قدامكوا هي الألطف على الإطلاق هي الألطف على + +142 +00:09:42,790 --> 00:09:46,050 +الإطلاق من الصور الموجودة على الجوجل يعني لو + +143 +00:09:46,050 --> 00:09:48,070 +اعملتوا google search على ال flash eating bacteria + +144 +00:09:48,070 --> 00:09:53,920 +هتشوفوا أشكال مخيفة كثيرة جدانفس البكتيريا اللى + +145 +00:09:53,920 --> 00:09:56,380 +تمالك تونسولايتل تمالك التهيبط اللواز هي نفس + +146 +00:09:56,380 --> 00:09:59,820 +البكتيريا اللى تمال فلش eating بكتيريا الفرق بينهم + +147 +00:09:59,820 --> 00:10:05,600 +ان هذى أصيبت ببكتيريوفاج فاكرين لبروفاج اللى بيعمل + +148 +00:10:05,600 --> 00:10:09,820 +integration لنفسه داخل البكتيريا اعطى البكتيريا + +149 +00:10:09,820 --> 00:10:15,060 +المقدرة على إنتاج لبروتيايز بكميات كبيرة بروتيايز + +150 +00:10:15,060 --> 00:10:19,520 +يعني او بروتيايز يعني أنزيمات هاضمة للبروتين + +151 +00:10:19,520 --> 00:10:24,570 +واللحم عبارة عن البروتينوبالتالي بنلاقي إنه ال + +152 +00:10:24,570 --> 00:10:28,270 +infection is very severe و ال consequences تبعتها + +153 +00:10:28,270 --> 00:10:32,910 +برضه بتكون damaging، يعني أحد الأشخاص المصابين في + +154 +00:10:32,910 --> 00:10:37,590 +الوجه بنشوف الأسنان من جوا، بصير destruction + +155 +00:10:37,590 --> 00:10:42,030 +لكميات كبيرة من ال muscles و من ال tissues و + +156 +00:10:42,030 --> 00:10:44,610 +بالتالي هو يعتبر من الأمراض الخطيرة و ال deadly + +157 +00:10:44,610 --> 00:10:49,130 +diseaseكلمة pyrogenic toxin أظن بيوجينيك غير عن + +158 +00:10:49,130 --> 00:10:53,250 +بيروجين بيروجين اللي هي fever producing و ال bio + +159 +00:10:53,250 --> 00:10:58,110 +gene هي ال pass producing ال pyrogenic toxin هو + +160 +00:10:58,110 --> 00:11:02,730 +يفر عن super antigen و كلمة super antigen يعني من + +161 +00:11:02,730 --> 00:11:06,490 +ميزاته أنه بيحفظ الخلايا على الإنقسام فبسموه + +162 +00:11:06,490 --> 00:11:10,210 +mitogen و هذا بيعمل activation لل immune system + +163 +00:11:10,210 --> 00:11:13,590 +أعتقد كلكوا شوفته أنه بيعمل non specific + +164 +00:11:13,590 --> 00:11:18,780 +activation لل immune systemفي مرض اسمه الحمّة + +165 +00:11:18,780 --> 00:11:22,980 +القرموزية أو scarlet fever مشهور برضه متسببه لـ + +166 +00:11:22,980 --> 00:11:27,420 +streptococcus biogenes ونتج عن مقدرة البكتيليا او + +167 +00:11:27,420 --> 00:11:30,580 +عن استرالة هالية على إنتاج توكسين اسمه eretrogenic + +168 +00:11:30,580 --> 00:11:36,760 +toxin الاسم تبع المرض أُخِذ منلون المريض، القرمزي، + +169 +00:11:36,760 --> 00:11:39,600 +لون المريض وفيه طبعا فيه fever وفيه بالإضافة اللي + +170 +00:11:39,600 --> 00:11:42,820 +هو ال rashes اللي انتوا شايفينها اللي قد تظهر على + +171 +00:11:42,820 --> 00:11:46,080 +لسان مش قد تظهر مميزة من ميزته انك تكون على لسان + +172 +00:11:46,080 --> 00:11:51,260 +المريض ايضا فيه rashes غير + +173 +00:11:51,260 --> 00:11:56,340 +infections غير قياحية هذه بدنا نفهمها كويس في سيقه + +174 +00:11:56,340 --> 00:12:02,260 +البكتيريا موجودة ممكن تعمل حاجة من تن تن تعمل + +175 +00:12:02,260 --> 00:12:03,380 +local infection + +176 +00:12:06,330 --> 00:12:10,610 +و ممكن تعمل الوكال سابيراتيف سابيراتيف يعني برضه + +177 +00:12:10,610 --> 00:12:13,810 +past producing نفس المعنى تبع بيوجينيك تمام؟ او + +178 +00:12:13,810 --> 00:12:20,070 +تان سابيراتيف لكن هذه البكتيريا نفسها لكن قد ينتج + +179 +00:12:20,070 --> 00:12:25,250 +أمراض عن موجود بقايا البكتيريا او عن آثار + +180 +00:12:25,250 --> 00:12:29,510 +البكتيريا، آثار المعركة اللي موجودة، حين تجانبها + +181 +00:12:29,510 --> 00:12:31,270 +رجزوا مياه و تقولوا الله و حلوة + +182 +00:12:34,040 --> 00:12:36,300 +الان ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +183 +00:12:36,300 --> 00:12:36,620 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +184 +00:12:36,620 --> 00:12:37,960 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +185 +00:12:37,960 --> 00:12:40,520 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +186 +00:12:40,520 --> 00:12:44,060 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +187 +00:12:44,060 --> 00:12:47,740 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +188 +00:12:47,740 --> 00:12:50,920 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +189 +00:12:50,920 --> 00:12:51,700 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +190 +00:12:51,700 --> 00:12:51,700 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +191 +00:12:51,700 --> 00:12:51,700 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +192 +00:12:51,700 --> 00:12:51,700 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +193 +00:12:51,700 --> 00:12:51,700 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +194 +00:12:51,700 --> 00:12:51,700 +ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال ال + +195 +00:12:51,700 --> 00:12:51,820 +ال ال ال + +196 +00:13:01,360 --> 00:13:06,180 +خلّينا من أول نقطة عرفنا إنه autoimmune disease + +197 +00:13:06,180 --> 00:13:09,960 +ناتج مش عن البكتيريا مباشرة وإنما عن reaction تبع + +198 +00:13:09,960 --> 00:13:14,300 +الاسم تبعنا ضد البكتيريا وبيطلقوا عليه autoimmune + +199 +00:13:14,300 --> 00:13:18,040 +disease لأن our own antibody attacks our own + +200 +00:13:18,040 --> 00:13:24,120 +antigen وبتسموه autoimmune diseaseبعنوان rheumatic + +201 +00:13:24,120 --> 00:13:27,540 +fever واخدوا بالكم rheumatic fever مش رهو + +202 +00:13:27,540 --> 00:13:30,960 +rheumatoid arthritis ال rheumatoid arthritis مرض + +203 +00:13:30,960 --> 00:13:34,740 +بيختلف عن ال rheumatic fever فال rheumatic fever + +204 +00:13:34,740 --> 00:13:39,920 +بنلاقي طفل أمره خمساشة عام أو عشرة أعوام من + +205 +00:13:39,920 --> 00:13:43,380 +repeated infection with streptococcus pyogenes + +206 +00:13:44,000 --> 00:13:49,700 +المفاصل تبعته عمرها تسعين سنة القلب تبعته عمره + +207 +00:13:49,700 --> 00:13:52,700 +تسعين سنة لأن ال antibodies بتهاجم أدلة القلب و + +208 +00:13:52,700 --> 00:13:56,120 +بتهاجم اللي هو ال myosin بالذات اللي في ورقة أدلة + +209 +00:13:56,120 --> 00:14:00,660 +القلب و بتهاجم المفاصل تبعت هذا المريض إذا هي + +210 +00:14:00,660 --> 00:14:05,440 +عبارة عن antibody attacking our own tissues وصل + +211 +00:14:05,440 --> 00:14:09,700 +الصورة قد ينتج عنها fever و heart disease و joint + +212 +00:14:09,700 --> 00:14:14,270 +inflammation و آخر جملة على اللوحة بتقولريوماتيك + +213 +00:14:14,270 --> 00:14:18,010 +fever not rheumatoid arthritis عشان نأكد أن دول + +214 +00:14:18,010 --> 00:14:21,570 +المرضين مختلفين تبعنا طب أساس هو ذات نفسه المرض + +215 +00:14:21,570 --> 00:14:27,650 +هدى ال fever مزمن ولا أنه rheumatoid مزمن؟ هو .. + +216 +00:14:27,650 --> 00:14:32,890 +هو بيعمل ال damage مزمن ال damage تبعه مزمن بمعنى + +217 +00:14:32,890 --> 00:14:37,000 +أخر damage يعني irreversibleيعني لما بيحضر من دم + +218 +00:14:37,000 --> 00:14:41,200 +العضلات أو من دم الانسجار في أدات القلب، قد يختفي + +219 +00:14:41,200 --> 00:14:44,020 +ال antibody بعد فترة، بيصيرله نوع من ال weighing + +220 +00:14:44,020 --> 00:14:49,340 +يعني بيقل تركيزه، لكن الظرر تبعه بيضل موجود لدرجة + +221 +00:14:49,340 --> 00:14:52,740 +أن ال rheumatic fever عن ال M-proteins كيف بأنه + +222 +00:14:52,740 --> 00:14:56,000 +بيعمل cross-reaction مع ال heart ميوسين وسمينا + +223 +00:14:56,000 --> 00:15:00,180 +وقلنا المصطلح ال disease autoimmunityفي مشكلة + +224 +00:15:00,180 --> 00:15:03,200 +تانية يا بنات، عندنا مشكلة تانية في ال .. ال .. ال + +225 +00:15:03,200 --> 00:15:06,200 +aesthetic orgasm، مش في الخلية نفسها و انما في + +226 +00:15:06,200 --> 00:15:11,340 +بقياها، poorly digested in vivo و persist + +227 +00:15:11,340 --> 00:15:15,510 +indefinitelyمية عن مية بتتكلم عن ال cell wall + +228 +00:15:15,510 --> 00:15:20,050 +antigens أنه ال digestion تبعها مش complete مش + +229 +00:15:20,050 --> 00:15:23,710 +complete يعني لا تتحلل إلى العناصر المكونة إلها + +230 +00:15:23,710 --> 00:15:30,810 +رقم اتنين it can persist تبقى indefinitely إيش + +231 +00:15:30,810 --> 00:15:34,090 +يعني indefinitely زي ما بنقول إحنا لأخر يعني مالاش + +232 +00:15:34,090 --> 00:15:38,210 +إلى مالة نهاية إلى مالة نهاية طب هذا إيش بيقدّي + +233 +00:15:38,210 --> 00:15:44,190 +بيقدّي إلى مشكلة أخرىتعالوا نطلع على المشكلة + +234 +00:15:44,190 --> 00:15:49,490 +الأخرى الـ Antigen لما بيرتبط بالـ Antibody فاكرين + +235 +00:15:49,490 --> 00:15:54,930 +بقولنا أن الـ binding قد يسبب ما يسمى complement + +236 +00:15:54,930 --> 00:16:00,570 +activation complement activation أذكركم الـ + +237 +00:16:00,570 --> 00:16:01,050 +complement + +238 +00:16:21,170 --> 00:16:25,330 +بقايا الانتيجنات مرتبطة بال antibodies و بتلف فى + +239 +00:16:25,330 --> 00:16:29,800 +الدم، لما تلف فى الدم مش الدم كله بيروح الكليةلما + +240 +00:16:29,800 --> 00:16:32,820 +هذا ال antigen-antibody complex كمان مرة نسميه + +241 +00:16:32,820 --> 00:16:36,920 +antigen-antibody complex و بنسميه هنا زى ما + +242 +00:16:36,920 --> 00:16:41,040 +شايفينها اللوحة immune complex immune complex ال + +243 +00:16:41,040 --> 00:16:43,980 +immune complexes كمان مرة هى ال antigen-antibody + +244 +00:16:43,980 --> 00:16:49,940 +binding بتترستر فى الكلمة طيب هو عبارة عن antigen + +245 +00:16:49,940 --> 00:16:53,800 +مرتبط ب antibody هى سبب activation لمين؟ لل + +246 +00:16:53,800 --> 00:16:57,480 +complement فال complement مفيش bacteria هتدمر مين؟ + +247 +00:16:57,760 --> 00:17:00,900 +هتدمر خلايا الكلية عشان هي اسم المرض acute + +248 +00:17:00,900 --> 00:17:06,100 +glomerul nephritis كمان مرة فيه بكتيريا هناك طيب + +249 +00:17:06,100 --> 00:17:10,340 +ال acute glomerul nephritis هيشهر المريض بإعراض ال + +250 +00:17:10,340 --> 00:17:13,680 +kidney infection لكن مافيش infection عملته مزرعة + +251 +00:17:13,680 --> 00:17:18,140 +negative ماعنده أي حاجة السبب هو مين؟ هو ال immune + +252 +00:17:18,140 --> 00:17:22,280 +complexes ومهاجمة ال complement كمان مرة لخلايا ال + +253 +00:17:22,280 --> 00:17:27,090 +kidney وزي الصورة إذا شفنا مرضينacute glomerul + +254 +00:17:27,090 --> 00:17:30,650 +nephritis وشوفنا rheumatic fever هدول التنتين او + +255 +00:17:30,650 --> 00:17:35,190 +التنين ليس لهم علاقة مباشرة بالبكتيريا وانما بال + +256 +00:17:35,190 --> 00:17:40,350 +immunity تبعتنا ضد اللي هو البكتيريا واحد ان cross + +257 +00:17:40,350 --> 00:17:44,450 +reaction يعني antibody بدل ما يتفاعل مع انتجين + +258 +00:17:44,450 --> 00:17:50,320 +واحد يتفاعل مع two او more antibodiesفمصطرح cross + +259 +00:17:50,320 --> 00:17:53,740 +reaction ما يكون عارفينه أنه مؤشر على أن ال + +260 +00:17:53,740 --> 00:17:57,820 +antibody أو ال antigen بتفاعل مع أكتر من نظيرهم + +261 +00:17:57,820 --> 00:18:03,700 +نيجي للميكروبط التاني خدوا بالكم من أهم الميكروبات + +262 +00:18:03,700 --> 00:18:06,360 +في ال streptococcus اللي ذكرناها في السابق ليها + +263 +00:18:06,360 --> 00:18:07,840 +streptococcus بيولينز + +264 +00:18:10,280 --> 00:18:13,620 +نقول إن ال group ممكن يكون فيها واحد أو أكتر، هذه + +265 +00:18:13,620 --> 00:18:19,080 +ال group دي لاتمتلك إلا عنصر واحد، اسمها + +266 +00:18:19,080 --> 00:18:20,860 +streptococcus agilis + +267 +00:18:23,710 --> 00:18:27,110 +عشان أكتبلكوا يعني الأمراض اللي بتسببها مكتوبة + +268 +00:18:27,110 --> 00:18:30,630 +neonatal meningitis septicemia و pneumonia أول مرة + +269 +00:18:30,630 --> 00:18:34,810 +نشوف ترتيب بعكوس تلاتة اتنين واحد يعني المفروض انه + +270 +00:18:34,810 --> 00:18:41,650 +واحدين تلاتة لكن هي الصحيح معتوطة ب .. بقصد ان + +271 +00:18:41,650 --> 00:18:45,470 +نقولكوا pneumonia هي البدء او نقطة الانطلاق لل + +272 +00:18:45,470 --> 00:18:49,490 +septicemia و ال septicemia هي نقطة الانطلاق لمين ل + +273 +00:18:49,490 --> 00:18:53,490 +neonatal meningitis لكن حاطناهم بالترتيباللي .. + +274 +00:18:53,490 --> 00:18:57,230 +اللي فوق لانه .. الأخطر هو مين؟ ال .. meningitis + +275 +00:18:57,230 --> 00:18:59,830 +أخطر من السيمية أخطر طبعا من اللي هو ال pneumonia + +276 +00:18:59,830 --> 00:19:04,070 +لكن واقع الأمر هي pneumonia بينشي عنها سبتسيمية + +277 +00:19:04,070 --> 00:19:09,030 +بينشي عنها قلنا meningitis في كمان مرض بيوصيب الأم + +278 +00:19:09,030 --> 00:19:13,410 +منها peripheral sepsis و أول مرة ممكن نقوله لأ + +279 +00:19:13,410 --> 00:19:18,140 +قلنا قبل هيكالpyrepralepsis هو حمّى النفاس، حمّى + +280 +00:19:18,140 --> 00:19:22,740 +النفاس، كنتوا تسمعوا .. مش تسمعوا، ممكن في زمن كوا + +281 +00:19:22,740 --> 00:19:26,260 +لأ مش موجود، بس كتير من النساء كانت موت بعد + +282 +00:19:26,260 --> 00:19:31,020 +الولادة بحمّى النفاس، و بقى جميعهم حمّى تلت أيام، + +283 +00:19:31,020 --> 00:19:33,540 +انت بقى كانت تيجي بعد تلت أيام من الولادة، بعد تلت + +284 +00:19:33,540 --> 00:19:38,260 +أيام من الولادةنسبة كبيرة جُنّا من الأمهات، مش أنا + +285 +00:19:38,260 --> 00:19:41,420 +بس في فلسطين، في العالم كله، لكن بعد ما صارت ال + +286 +00:19:41,420 --> 00:19:44,560 +infection control measures و ال antibiotics، هذه + +287 +00:19:44,560 --> 00:19:49,860 +تقريبا وصلت أنا إلى zero وفيات الأمهات خلال + +288 +00:19:49,860 --> 00:19:55,920 +الولادة، الناجمة عن ال peripheral sepsisطيب هذا من + +289 +00:19:55,920 --> 00:19:59,560 +الميكروبات المهمة في فلسطين و انا كمان مرة بقول + +290 +00:19:59,560 --> 00:20:05,680 +انه بنحاول قدر الإمكان أنه ننبه الأطباء خاصة أطباء + +291 +00:20:05,680 --> 00:20:09,140 +النساء والولادة إيه أهمية هذا الميكروب في لعناية + +292 +00:20:09,140 --> 00:20:14,300 +الأمومة و الطفولة بنقولهم التالي، بنقولهم الست + +293 +00:20:14,300 --> 00:20:17,840 +الحامل في الشهر السادس و ثم فوق إيه اللي بقى أن + +294 +00:20:17,840 --> 00:20:23,490 +تخضع لفحص؟هذا الفحص لموجود أو عدم موجود ل group B + +295 +00:20:23,490 --> 00:20:26,450 +streptococci الاسمها streptococcus agalactica من + +296 +00:20:26,450 --> 00:20:33,830 +vaginal swab مزرعة و بنتعرف على البكتيريا موجودة + +297 +00:20:33,830 --> 00:20:38,170 +بنحط بس تشك إنها positive مش مولودة بنحط تشك إنها + +298 +00:20:38,170 --> 00:20:41,910 +negative مابنعملاش ولا أي إجراء و بننتظر ليوم + +299 +00:20:41,910 --> 00:20:46,070 +الولادة يوم الولادة بيعطي ما يسمى بالكلمة خلال + +300 +00:20:46,070 --> 00:20:52,050 +الولادة معناها intrapartumهذا مصطلح بيطلق على خلال + +301 +00:20:52,050 --> 00:20:55,550 +الولادة فلو أعطينا Antibiotic زي البانسالين خلال + +302 +00:20:55,550 --> 00:21:00,260 +الولادة إحنا نضرب على الصفرانج بحاجةنحمي الأم من + +303 +00:21:00,260 --> 00:21:03,100 +الـ Periperal Sepsis لأن انت عارفين عمليات الولادة + +304 +00:21:03,100 --> 00:21:09,020 +عملية عنيفة دم و نزف وجروح النيوكاس من بلاي تتهتك + +305 +00:21:09,020 --> 00:21:13,500 +كتير منها يعني عملية عنيفة بالفعل و الدم اللي بنزل + +306 +00:21:13,500 --> 00:21:17,060 +هذا نزل من كبلارز و من .. من .. مظبوط وبالتالي في + +307 +00:21:17,060 --> 00:21:21,180 +إمكانية أنه يصير تلوث و أن البكتيريا قصرت دم عند + +308 +00:21:21,180 --> 00:21:27,360 +الأم اللي هي في الولادة فوجودantibiotic بيحمي الأم + +309 +00:21:27,360 --> 00:21:33,300 +من البرولاسيبسيس وبيحمي الطفل المولود من انه يعمل + +310 +00:21:33,300 --> 00:21:37,100 +.. يصير لو عنده colonization استعمار من قبل ال + +311 +00:21:37,100 --> 00:21:41,000 +group B streptococci للطفل وابتدي لان يحدث نيمونيا + +312 +00:21:41,000 --> 00:21:45,040 +ولا يحدث سيبسيميا ولا يحدث ايه .. من .. للأسف ال + +313 +00:21:45,040 --> 00:21:47,740 +practice هذا غير موجود البتة + +314 +00:21:52,160 --> 00:21:55,780 +في قطاع غزة مش مولود البتة وبالتالي نأمل من الله + +315 +00:21:55,780 --> 00:22:00,320 +انه ينتبه لهذا الموضوع ليش احنا ركزنا على ست + +316 +00:22:00,320 --> 00:22:04,820 +الحامل؟ لأنه 20 ل 30% من الستات بيحتووا على هذا + +317 +00:22:04,820 --> 00:22:09,500 +الميكروب ك normal microbiota normal microbiota + +318 +00:22:09,500 --> 00:22:13,360 +يعني هو غير مضر اللي هم خلال قبل الولادة لكن يوم + +319 +00:22:13,360 --> 00:22:18,120 +الولادة بيصير مضر لا الست ولا اللي هو المولود اللي + +320 +00:22:18,120 --> 00:22:22,050 +طالبإذا ال transmission هو عبر ال vagina الفلورة + +321 +00:22:22,050 --> 00:22:25,450 +during birth during birth أنت بتعرف اليوم صار في + +322 +00:22:25,450 --> 00:22:27,790 +حين اسمها caesarian section اللي هو الولادة + +323 +00:22:27,790 --> 00:22:33,250 +القيصرية وصارت شائعة بس أنبقى لكوا نقطة البعض يظن + +324 +00:22:33,250 --> 00:22:39,410 +وكان يظن أن الولادة القيصرية بتحمي الطفل من ال + +325 +00:22:39,410 --> 00:22:43,610 +infection لأن بمارش عبر مين؟ عبر ال vagina اللي هي + +326 +00:22:43,610 --> 00:22:47,140 +مليانة لهم الملك اللي هو بيوتها صح؟لكن وردوا انه + +327 +00:22:47,140 --> 00:22:52,560 +.. وردوا انه نسبة الإصابة للأطفال اللي بيولدوا من + +328 +00:22:52,560 --> 00:22:56,100 +ال C-section اللي هو cesarean section هذا يأما + +329 +00:22:56,100 --> 00:23:01,100 +مساوية للأطفال اللي بيولدوا دره الطبيعية أو حتى + +330 +00:23:01,100 --> 00:23:04,440 +أعلى منها في دراسة احنا عملناها مع طالب بالستر و + +331 +00:23:04,440 --> 00:23:08,460 +أظهر انه الناس اللي بيولدوا من ال C-section نسبة + +332 +00:23:08,460 --> 00:23:12,960 +ال neonatal septicemia عندهم أعلى من الأطفال اللي + +333 +00:23:12,960 --> 00:23:15,640 +بيولدوا normal delivery + +334 +00:23:18,270 --> 00:23:21,170 +سألت سؤال احنا ماجدراش نجاوب عليه في ال research + +335 +00:23:21,170 --> 00:23:24,950 +لإن هو سؤال محير المنطق أن يكون العكس .. المنطق أن + +336 +00:23:24,950 --> 00:23:29,530 +يكون العكس لكن يبدو أن ال procedures .. ال .. ال + +337 +00:23:29,530 --> 00:23:32,510 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +338 +00:23:32,510 --> 00:23:32,770 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +339 +00:23:32,770 --> 00:23:33,090 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +340 +00:23:33,090 --> 00:23:33,090 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +341 +00:23:33,090 --> 00:23:37,330 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +342 +00:23:37,330 --> 00:23:37,330 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +343 +00:23:37,330 --> 00:23:38,030 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +344 +00:23:38,030 --> 00:23:38,030 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +345 +00:23:38,030 --> 00:23:39,030 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +346 +00:23:39,030 --> 00:23:41,470 +ال .. ال .. ال .. + +347 +00:23:44,100 --> 00:23:48,640 +من الأول اللي بيطلع .. اول نفس بياخده .. اول .. + +348 +00:23:48,640 --> 00:23:52,200 +اول ايد تمسكه بتعطيه micro organism .. تمام؟ من + +349 +00:23:52,200 --> 00:23:56,160 +اول ملامسها الجلد .. جلد الأم عند الرضاعة أو ال + +350 +00:23:56,160 --> 00:24:00,260 +bottle تبع الحليب بياخد micro organism طبعا مطلوب + +351 +00:24:00,260 --> 00:24:03,020 +انه ياخد micro organism هو بالعكس انا اقوللكوا ان + +352 +00:24:03,020 --> 00:24:06,780 +ال micro bad في هذه الحالة اذا كانت في environment + +353 +00:24:06,780 --> 00:24:11,910 +healthy بتكون مفيدة لأطفالاللي انا بدأ اقوله ان + +354 +00:24:11,910 --> 00:24:16,370 +احنا البعض مننا بيسوق لمين لـ caesarian section لأ + +355 +00:24:16,370 --> 00:24:19,630 +بيقولكوا caesarian section فقط المفروض فقط في + +356 +00:24:19,630 --> 00:24:24,970 +حالات الولادة المتعثرة مش يعني .. مش افضل من ال + +357 +00:24:24,970 --> 00:24:31,350 +normal delivery طيب strip to caucas a galacti + +358 +00:24:31,350 --> 00:24:35,770 +هويته ايطالية برضه strip to caucas a galacti طبعا + +359 +00:24:35,770 --> 00:24:39,870 +فيه افتصار اللي هي ال S نقطة + +360 +00:24:48,120 --> 00:24:50,640 +عشان هيك كنت اتعرف ان الاقتصاد بتتشابه هى + +361 +00:24:50,640 --> 00:24:53,680 +الاستفيلو كوكاس مع الاستفيلو كوكاس التانى اجه و + +362 +00:24:53,680 --> 00:24:57,380 +حرف اللى هو ال S طيب الصورة اللى قدامنا هي صورة + +363 +00:24:57,380 --> 00:25:01,840 +رائعة جدا و هتضطر تخلينا نتكلم موضوع خارج نطاق + +364 +00:25:01,840 --> 00:25:09,360 +المحاضرة لكن برضه في ال micro biology لما + +365 +00:25:09,360 --> 00:25:13,060 +بنقول واحد زاد واحد احنا غالبا الجواب اللى بتبادل + +366 +00:25:13,060 --> 00:25:17,720 +للدهن عننا ع طول اتنين مش غالبا دائماإتنين هذا دي + +367 +00:25:17,720 --> 00:25:22,460 +المعرفة ان واحد زاد واحد يساوي اتنين طيب انجليها + +368 +00:25:22,460 --> 00:25:26,160 +في صيغة تانية انت بتكتب 100 كلمة في الدقيقة ويا + +369 +00:25:26,160 --> 00:25:30,580 +بتكتب 100 كلمة في الدقيقة لو قلنالكوا اكتبوا كلمات + +370 +00:25:30,580 --> 00:25:35,480 +مجموع كلماتكوا هيطلع كدهش في الدقيقة يبقى واحد زاد + +371 +00:25:35,480 --> 00:25:39,540 +واحد يساوي اتنين صح ولا لأ طيب لو قلناهم اكتبوا + +372 +00:25:39,540 --> 00:25:45,420 +مرة تانية مثال تاني تنتين غير مية ومية فواحدة منهم + +373 +00:25:45,420 --> 00:25:51,260 +شغلت على التانيةيعني عاقة عملها بدل ما التنتين + +374 +00:25:51,260 --> 00:25:57,420 +يكتبوا 200 يمكن يكتبوا عاقة على عملها ممكن يكتبوا + +375 +00:25:57,420 --> 00:26:02,340 +اقل من 100 او اقل من 200 لكن في الحالة واحد زياد + +376 +00:26:02,340 --> 00:26:06,100 +واحد ده يساوي اتنين لان في تبعض في ال action نجي + +377 +00:26:06,100 --> 00:26:11,910 +لأ مثال تالتنفسي لو شخصين كنت لهم اكتبوا مية مية + +378 +00:26:11,910 --> 00:26:16,990 +تعوانوا مع بعض جالهم .. حكت انه الكلمات هذه + +379 +00:26:16,990 --> 00:26:20,490 +المقررة اللي انا بعملها او انا عندي طريقة أسرع على + +380 +00:26:20,490 --> 00:26:24,810 +الكتابة خدي تعلمي التقنية هذه، المهم في الآخر لما + +381 +00:26:24,810 --> 00:26:28,130 +جينا جمعنا الكلمات لجينا لهم تلات مية، واحد زاد + +382 +00:26:28,130 --> 00:26:30,410 +واحد بطلوا .. بطلوا اتنين، بطلوا اتنين، صاروا + +383 +00:26:30,410 --> 00:26:35,150 +تلاتةففي عندنا في العلاقات في المُركّبات لما + +384 +00:26:35,150 --> 00:26:38,050 +بيقوموا ب action واحد يعني مُركّبين هم جايبين + +385 +00:26:38,050 --> 00:26:42,330 +بيطبعوا كلمات مُركّبين بيعملوا homolysis واحد + +386 +00:26:42,330 --> 00:26:45,750 +بيعمل homolysis وضفناله كمان واحد بيعمل homolysis + +387 +00:26:45,750 --> 00:26:50,130 +المنطق انه واحد زاد واحد ساوي اتنين واحد ده موجود + +388 +00:26:50,130 --> 00:26:52,890 +لكن هناك بعض الأحيان بنلاجأ حالة اسمها synergism + +389 +00:26:52,890 --> 00:26:59,530 +تآذر تآذر يعني تعاون وبيسميها synergism كمان + +390 +00:26:59,530 --> 00:27:07,340 +مرضياش؟Synergism, Antagonism and Addition نعرفهم + +391 +00:27:07,340 --> 00:27:14,040 +بالأرقام ونقول synergism واحد زائد + +392 +00:27:14,040 --> 00:27:20,840 +واحد يساوي هو المفروض ان نقول الدقيقة اكثر من + +393 +00:27:20,840 --> 00:27:25,460 +اتنين لكن لنحفظهم ونسميهم تلاتة Antagonism واحد + +394 +00:27:25,460 --> 00:27:30,520 +زائد واحد يساوي واحد ال addition واحد + +395 +00:27:34,240 --> 00:27:37,580 +هيك بيكون هنا اجددنا انه نعرف العلاقات بين + +396 +00:27:37,580 --> 00:27:41,200 +مُركّبين بيقوم نفس الوظيفة وين بهمنا الكلام هذا؟ + +397 +00:27:41,200 --> 00:27:45,940 +بيهمنا كمان في ال antibiotics في ال antibiotics في + +398 +00:27:45,940 --> 00:27:50,940 +عندنا antibiotic قد يُعطى in combination مع أخر + +399 +00:27:50,940 --> 00:27:56,590 +antibioticممكن للأسف .. للأسف إذا ماكناش عارفين + +400 +00:27:56,590 --> 00:27:59,510 +العلاقات الثلاثة هدول اللي حكينا عنهم نعطي اتنين + +401 +00:27:59,510 --> 00:28:03,810 +يعملوا تضاد بدل ما يساعدوا بعض و يخففوا من ال .. + +402 +00:28:03,810 --> 00:28:07,150 +من .. من وقت ال .. العلاج أو يقلوا وقت العلاج لأ + +403 +00:28:07,150 --> 00:28:09,870 +ممكن يعملوا تضاد و يزيدوا .. و يزيدوا من حتة دي + +404 +00:28:09,870 --> 00:28:14,310 +المشكلة و بالتالت نعرف المصطلحات التلاتة synergism + +405 +00:28:14,310 --> 00:28:17,970 +تأذر antagonism تضاد و addition اللي هو قلنا عنه + +406 +00:28:17,970 --> 00:28:20,850 +ال .. ال .. الإضافة بسميها إضافة + +407 +00:28:26,040 --> 00:28:29,680 +طيب، إيش اللي خدناه يتكلم بهذا الكلام؟ اللي خدناه + +408 +00:28:29,680 --> 00:28:35,220 +يتكلم بهذا الكلام هو وجود علاقة وطيدة ما بين الـ + +409 +00:28:35,220 --> 00:28:39,860 +Beta-Hemolysin الخاص بالـ Stratococcus Agalactica + +410 +00:28:39,860 --> 00:28:43,000 +والـ Beta-Hemolysin تبع الـ Stratococcus Aureus + +411 +00:28:43,830 --> 00:28:47,690 +جالولنا ان ال Beta-Hemolysin بتاع الأجلاكتيا جامع + +412 +00:28:47,690 --> 00:28:54,050 +سينارجزم سينارجزم يعني تاثر مع ال Staphylococcus + +413 +00:28:54,050 --> 00:28:57,550 +aureus ولحظوا الرسم اللي قدامكم هذه البكتيريا اللي + +414 +00:28:57,550 --> 00:29:01,010 +مزروعة هنا على الخط الطويل هذا هي Staphylococcus + +415 +00:29:01,010 --> 00:29:06,490 +aureus في بكتيريا أخرى زرعناها بشكل عمودي متعامد + +416 +00:29:06,490 --> 00:29:11,050 +عليها اللي هي Staphylococcus Agalactica وواحدة كنا + +417 +00:29:11,050 --> 00:29:14,150 +شاكين أنها تكون Staphylococcus Agalacticaلحظوا + +418 +00:29:14,150 --> 00:29:19,430 +منطقة الالتقاء بين التنين ايش عملت؟ عملت ما يسمى + +419 +00:29:19,430 --> 00:29:22,870 +Arrowhead Formation عملت زي رأس السهن Arrowhead + +420 +00:29:22,870 --> 00:29:26,570 +Formation يعني ال zone .. ال zone تبعت ال + +421 +00:29:26,570 --> 00:29:29,830 +hemolysis زادت ولا نجست؟ زادت وبالتالي هذا + +422 +00:29:29,830 --> 00:29:33,590 +scenarioism ولا أنتجونيزم؟ scenarioism هذا الصفة + +423 +00:29:33,590 --> 00:29:38,270 +.. هذا الصفة تعتبر صفة مميزة للـ streptococcus أجل + +424 +00:29:38,270 --> 00:29:43,420 +أجل .. إذن أحد أهم الصفات اللي بنعرف من خلالهاالـ + +425 +00:29:43,420 --> 00:29:47,220 +Galactia هي الصفحة دي ايش اسم الصفحة دي camp test + +426 +00:29:47,220 --> 00:29:53,700 +camp test أو camp reaction طبعا camp هي عبارة عن + +427 +00:29:53,700 --> 00:29:58,860 +أربع أحرف لأربع علماء شاركوا فيها ده ده ده فالمسمي + +428 +00:29:58,860 --> 00:30:05,100 +هنا camp reaction أو camp test و انتهى الموضوع طيب + +429 +00:30:05,100 --> 00:30:10,560 +البكتيريا التانية هذه اللي تحت عملت synergism؟ + +430 +00:30:13,150 --> 00:30:17,570 +يبقى مستهير تكون أجلكت طب في فحص تاني اسمه + +431 +00:30:17,570 --> 00:30:21,330 +hypurate hydrolysis، hypurate hydrolysis، لحظة + +432 +00:30:21,330 --> 00:30:26,390 +الأنابيب اللي هنا، في عندي واحدة لونها تقريبا + +433 +00:30:26,390 --> 00:30:33,270 +نفسي، واحدة colorless، تمام؟ ال .. ال step to + +434 +00:30:33,270 --> 00:30:37,670 +caucus أجلكت هي hypurate hydrolysis positive، يعني + +435 +00:30:37,670 --> 00:30:39,430 +بتعمل hydrolysis لمين؟ + +436 +00:31:00,230 --> 00:31:05,870 +هل هذه المعلومات الآن غريبة عليك؟كلها مرت الآن + +437 +00:31:05,870 --> 00:31:10,190 +كلها مرت الآن انا بحب دايما اقول streptococcus + +438 +00:31:10,190 --> 00:31:13,910 +agalactia او اي ميكروب ببدأه من الاخر + +439 +00:31:13,910 --> 00:31:16,270 +streptococcus agalactia كيف عرفت انه streptococcus + +440 +00:31:16,270 --> 00:31:20,730 +agalactia لانه كان test positive لان الهيبيوريتا + +441 +00:31:20,730 --> 00:31:23,010 +هيدوله test positive طب انا ليش عملت اول فحصين + +442 +00:31:23,010 --> 00:31:25,910 +هدوله لانهم كانوا catalase negative طب انا ليش + +443 +00:31:25,910 --> 00:31:28,670 +عملت فحصين catalase أساسا لانها gram positive + +444 +00:31:28,670 --> 00:31:33,610 +كوكسايل لانها gram positive كوكسايلفعملية الصعود + +445 +00:31:33,610 --> 00:31:36,950 +من أسفل إلى أعلى أو من أقل إلى أسفل بحيث أنه + +446 +00:31:36,950 --> 00:31:40,610 +بالفعل يصير لنا نوع من ال characterization وكلمة + +447 +00:31:40,610 --> 00:31:44,330 +characterization يعني identification و أحد أهم + +448 +00:31:44,330 --> 00:31:48,170 +نقاط عملنا + +449 +00:31:48,170 --> 00:31:52,190 +في ال microbiology هو العزل و التعرف على ال + +450 +00:31:52,190 --> 00:31:53,390 +microorganism + +451 +00:32:03,890 --> 00:32:08,170 +لأ لأ انا بتكلم عن two organisms اللي نفترض انه + +452 +00:32:08,170 --> 00:32:11,910 +بيعمله the same action killing the bacteria طبعا + +453 +00:32:11,910 --> 00:32:14,550 +بيقتله بكتيريا زي ال antibiotic زي ال antibiotic, + +454 +00:32:15,090 --> 00:32:18,610 +antibiotic X و لنفترض انه penicillin و antibiotic + +455 +00:32:18,610 --> 00:32:23,280 +Y و لنفترض انه تتراcyclineهدول الاتنين لو حطيناهم + +456 +00:32:23,280 --> 00:32:27,960 +مع بعض تمام ال tetracycline بيأثر على عمل مين + +457 +00:32:27,960 --> 00:32:32,100 +البنثالين فبدل ما واحد زائد واحد يساوي اتنين ايش + +458 +00:32:32,100 --> 00:32:37,200 +بيصير الان واحد زائد واحد يساوي واحد لكن لو غيّرنا + +459 +00:32:37,200 --> 00:32:43,460 +التتراcycline وحطينا بداله جندا ومايسينالجنتوايسين + +460 +00:32:43,460 --> 00:32:47,100 +بيتعذر مع ال penicillin و بدل ما واحد زاد واحد + +461 +00:32:47,100 --> 00:32:50,580 +يساوي اتنين بس واحد زاد واحد يساوي اتنين ثلاثة هذا + +462 +00:32:50,580 --> 00:32:54,100 +الكلام ننقله على مين؟ على ال hemolysin hemolysin و + +463 +00:32:54,100 --> 00:32:58,840 +hemolysin زادوا من قدرة اللي هو واحد زاد من قدرة + +464 +00:32:58,840 --> 00:33:04,120 +الأخر وصلت؟ انا + +465 +00:33:04,120 --> 00:33:07,760 +بدي اعطي مثال على ال hemolysin هو عبارة عن ايش؟ + +466 +00:33:07,760 --> 00:33:11,400 +عبارة عن تكسين ال hemolysin عبارة عن تكسينبدأي + +467 +00:33:11,400 --> 00:33:16,340 +بمثال واضح جدا على two antibiotics اللي هم تأثير + +468 +00:33:16,340 --> 00:33:21,760 +تأذري وعبارة تعرف ال pathway؟ ايش يعني pathway؟ + +469 +00:33:21,760 --> 00:33:26,840 +يعني مصار metabolism، مصار metabolism المصار + +470 +00:33:26,840 --> 00:33:30,580 +المتابوليزم عبارة عن خطوات واحد، اتنين، تلاتة، + +471 +00:33:30,580 --> 00:33:35,680 +اربعة، خمسة واحد من ال antibiotic بيؤثر، بيوجّف + +472 +00:33:35,680 --> 00:33:41,520 +المصار عند النقطة هذه، تمام؟و بي .. و بي .. و بي + +473 +00:33:41,520 --> 00:33:45,680 +.. و بيقتل البكتيريا لأنه بيوقف ال .. ال .. ال .. + +474 +00:33:45,680 --> 00:33:48,100 +ال metabolism عند النقطة هذه فما بتصارش للنقطة + +475 +00:33:48,100 --> 00:33:52,640 +الأخيرة تمام؟ إيجي Antibiotic تاني استخدمناه بيوقف + +476 +00:33:52,640 --> 00:33:57,600 +النقطة هذه في المسار بيوقف النقطة التانية صاروا + +477 +00:33:57,600 --> 00:34:01,100 +التانين بيشتغلوا على نفس المسار لكن على حطوات + +478 +00:34:01,100 --> 00:34:05,760 +مختلفة الأهم دلوقتي بتتخيل أنه عندي أنا ال pathway + +479 +00:34:05,760 --> 00:34:09,080 +مجون من مجموعة من ال enzymes و ال substrate صح ولا + +480 +00:34:09,080 --> 00:34:13,820 +لأ؟وحد هرب من ال antibiotic هذا لكن لن يهرب من + +481 +00:34:13,820 --> 00:34:16,980 +مين؟ من ال antibiotic التاني وصل الصورة لبصير في + +482 +00:34:16,980 --> 00:34:20,240 +عندها في هذا الحالة اللي هو ال action مضاء اكتر من + +483 +00:34:20,240 --> 00:34:24,260 +الضعف تبع الانش يبقى بيشتغل على different steps في + +484 +00:34:24,260 --> 00:34:29,400 +pathway معين زي ال toxin زي ال hemolysis بقى طيب + +485 +00:34:30,440 --> 00:34:33,720 +عندي group D streptococcus و هنفتن فيها محاضرتنا + +486 +00:34:33,720 --> 00:34:38,300 +اليوم group D streptococcus من ال microbat برضه + +487 +00:34:38,300 --> 00:34:42,160 +اللي احنا قلناه مهم يقولنا A وB وD مظبوط A B D + +488 +00:34:42,160 --> 00:34:46,400 +أخدنا A بيوجينز أخدنا B أجلاكتيا هتاخد الآن بعض + +489 +00:34:46,400 --> 00:34:53,680 +المعلومات على group Dأحد أهم مميزاتها، صفاتها، من + +490 +00:34:53,680 --> 00:34:57,720 +خلالها بممكن تتعرف عليها مقدرتها على النمو فيما + +491 +00:34:57,720 --> 00:35:04,420 +يسمى بالـ Biesquilin Agar الـ Biesquilin Agar هذا + +492 +00:35:04,420 --> 00:35:08,200 +ضامم صفتين في بعض، تعرفوا الـ Bile؟ + +493 +00:35:11,520 --> 00:35:17,560 +أو صارت الصفراء فاكرين المقومكي كيف صار specific + +494 +00:35:17,560 --> 00:35:22,460 +أو selective؟ صار selective لأنه أضافنا عليه + +495 +00:35:22,460 --> 00:35:28,440 +crystal violet و bile source يبقى ال bile القاتل + +496 +00:35:28,440 --> 00:35:33,220 +للإجرام بوستر قاتل للإجرام بوستر طفلات البكتيريه + +497 +00:35:33,220 --> 00:35:33,660 +للإجرام بوستر + +498 +00:35:43,550 --> 00:35:48,710 +بالظبط وبالتالي صفتها صفتها من صفتها اللي عندها + +499 +00:35:48,710 --> 00:35:53,570 +المقدرة على تنمو في ميل في ال bio على عكس معظم ال + +500 +00:35:53,570 --> 00:35:59,430 +gram-busted بكتيريا فهذا فحص مميز إليها، الآن هي + +501 +00:35:59,430 --> 00:36:02,970 +مش بس بتنمو في ال bio و عندها المقدرة على استغلال + +502 +00:36:02,970 --> 00:36:11,320 +مركب اسمه Esculin فإذا البكتيريا نمتعلى الوصف هذا + +503 +00:36:11,320 --> 00:36:16,040 +فهي تمتلك الصفتين تمتلك صفة إنها قادرة إنها تنمو + +504 +00:36:16,040 --> 00:36:20,060 +في ال بال و قادرة تستغل من الاسكلين يعني مش صفة + +505 +00:36:20,060 --> 00:36:25,480 +واحد صفتين و عندها المقدرة إنها تنمو في 6.5% صلاة + +506 +00:36:25,480 --> 00:36:33,500 +إذا نمت في ال 6.5%فهي enterococcus إذا نمتش في ال + +507 +00:36:33,500 --> 00:36:37,280 +.. في الستة و نص فهي non-enterococcus إذا ال group + +508 +00:36:37,280 --> 00:36:41,280 +دي stripped cocaine الآن انجسمت لجسمين مين انجسمت + +509 +00:36:41,280 --> 00:36:45,940 +لجسمين؟ مقدرة البكتيريا على النمو في ستة و نص + +510 +00:36:45,940 --> 00:36:50,260 +ليكون percent sodium chloride إذا نمت فهي + +511 +00:36:50,260 --> 00:36:54,760 +enterococcus وإذا ما نمت فهي non-enterococcus إذا + +512 +00:36:54,760 --> 00:36:57,180 +ال group دي stripped cocaine فيها .. فيها نوعين + +513 +00:36:57,180 --> 00:37:01,290 +enterococcus و non-enterococcusو ايه المهمة الـ + +514 +00:37:01,290 --> 00:37:06,950 +Enterococcus اكتبوا عنكوا على لوحة بيبقاتكوا بالخط + +515 +00:37:06,950 --> 00:37:12,610 +العريض VRE Vancomycin + +516 +00:37:12,610 --> 00:37:21,490 +Resistant Enterococci VRE V R E Vancomycin + +517 +00:37:21,490 --> 00:37:28,290 +Resistant Enterococci اسمه ماعرفش مهمة المعلوم هي + +518 +00:37:28,290 --> 00:37:33,600 +VREانتوا بتعرفوا ان احنا تاريخنا عن ال antibiotic + +519 +00:37:33,600 --> 00:37:39,180 +تاريخ طويل صارله تقريبا يعني من الاربعينيات ستين و + +520 +00:37:39,180 --> 00:37:44,100 +عشرين يعني تقريبا تمانين سنة تمانين سنة هذا + +521 +00:37:44,100 --> 00:37:50,360 +التاريخ قرفوله لطباء بمصطلح عصر المضادات الحيوية + +522 +00:37:50,360 --> 00:37:53,660 +تخيلوا ان احنا في يوم من أيام نسمي فترة زمانية + +523 +00:37:53,660 --> 00:37:58,480 +معينة بالمضادات الحيوية عصر المضادات الحيويةطب + +524 +00:37:58,480 --> 00:38:02,000 +علشان أسئلة وليش أهميتها؟ احنا قبل شهر كنا نتكلم + +525 +00:38:02,000 --> 00:38:04,800 +عن ال puripral sepsis و كثير من الأمهات كانت موت + +526 +00:38:04,800 --> 00:38:10,440 +من حمض نفس لكن مع وجود ال antibiotics قلت فبالتالي + +527 +00:38:10,440 --> 00:38:13,380 +ال antibiotics بالفعل أحدثت ثورة كبيرة في العلاج و + +528 +00:38:13,380 --> 00:38:17,900 +في ال preventionالان ال antibiotics كانت رصيد مهم + +529 +00:38:17,900 --> 00:38:21,400 +جدا للأسف قصي استغلال وصار استخدمه في مجالات + +530 +00:38:21,400 --> 00:38:26,080 +متنوعة في تغذية الدواجن في علاج للحيوانات وبالتالي + +531 +00:38:26,080 --> 00:38:30,820 +بدأت البكتير يتكون له resistance ال vancomycin أحد + +532 +00:38:30,820 --> 00:38:34,380 +أهم ال antibiotics اللي كنا حاطينهم على الجهة + +533 +00:38:34,380 --> 00:38:39,950 +حاطينهم على العوزة تمام؟ليش؟ لأنه بدأت ال gram + +534 +00:38:39,950 --> 00:38:42,750 +-bosted بكتيريا تكون resistant للبنسلينات والـ + +535 +00:38:42,750 --> 00:38:46,350 +cephalosporins و ال vancomycin is very effective + +536 +00:38:46,350 --> 00:38:51,690 +against gram-bosted بكتيريا فوصفته ال WHO بال + +537 +00:38:51,690 --> 00:38:58,350 +vancomycin انه آخر رصاصة آخر رصاصة في ترسانتها ضد + +538 +00:38:58,350 --> 00:39:01,830 +ال gram-bosted بكتيريا في فترة من الفترات لاحظوا + +539 +00:39:01,830 --> 00:39:08,990 +الوصف، وصف دقيق فلقة أو رصاصة و ترسانةعسكرية لأن + +540 +00:39:08,990 --> 00:39:14,530 +ما يدور بيننا وبين الميكروبات هو حقا حالة حرب.الآن + +541 +00:39:14,530 --> 00:39:20,630 +تخيلوا في غزة بدأنا نعزل كميات ضخمة بنسب مول يعني + +542 +00:39:20,630 --> 00:39:23,890 +يمكن قبل الفين و تمانية .. الفين و تمانية ما بطلتش + +543 +00:39:23,890 --> 00:39:26,710 +اللي احنا كام سنة، نحن ست سنوات، يعني قبل ست سنوات + +544 +00:39:26,710 --> 00:39:31,730 +عزلنا ما نسبته .. ما نسبته اربعين في المية من ال + +545 +00:39:31,730 --> 00:39:35,270 +enterococci في المستشفى كانت resistant لل + +546 +00:39:35,270 --> 00:39:41,210 +Vancomycin، بتوقع اليومالوضع أصعب، في البحر بنعمل + +547 +00:39:41,210 --> 00:39:45,210 +دراسة حالية برضه نسبة والله يمكن 60% لحد الآن + +548 +00:39:45,210 --> 00:39:47,610 +للأرقام اللي .. اللي .. اللي .. اللي لحد الآن + +549 +00:39:47,610 --> 00:39:51,650 +تتوفر هنا 60% من العزلات تقريبا resistant ل + +550 +00:39:51,650 --> 00:39:57,160 +الentero .. للعفو .. للبنكو myselfلدينا مشكلة + +551 +00:39:57,160 --> 00:40:00,680 +حقيقية لما انا اقول VRA .. VRA مانا احكي ان + +552 +00:40:00,680 --> 00:40:04,140 +الطالجة الأخيرة او الرصاصة الأخيرة اللي كنا راحت + +553 +00:40:04,140 --> 00:40:08,840 +فبتالي هتضطر نلجأ ل extreme measures و ندفع فلوس + +554 +00:40:08,840 --> 00:40:11,780 +تخبرنا ان انتهى ال new antibiotics اللي هي مش + +555 +00:40:11,780 --> 00:40:15,040 +بالسهل أصبحنا كل عشر سنوات لما نكتشف أن ال + +556 +00:40:15,040 --> 00:40:16,900 +antibiotic مناسب + +557 +00:40:26,310 --> 00:40:28,010 +و نكمل المرة القادمة ان شاء الله + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/VIGilQ1fnrY_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/VIGilQ1fnrY_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..10cb8e8982ab87b51a1af0a61d9e32614a6d5042 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/VIGilQ1fnrY_raw.srt @@ -0,0 +1,2584 @@ +1 +00:00:20,940 --> 00:00:22,640 +بسم الله الرحمن الرحيم الحمد لله رب العالمين + +2 +00:00:22,640 --> 00:00:25,780 +والصلاة والسلام وعلى سيدنا محمد وعلى آله وصحبه ومن + +3 +00:00:25,780 --> 00:00:30,500 +ساعة دربه اليوم الدين هذا يوم ختم محاضرتنا ان شاء + +4 +00:00:30,500 --> 00:00:34,020 +الله نعمل من الله انه يكون كان فصل دراسي مثمر + +5 +00:00:34,020 --> 00:00:39,220 +واستفدنا منه معلومات تؤهلنا إلى المرحلة التانية + +6 +00:00:39,220 --> 00:00:43,020 +اللي هي مرحلة diagnostic microbiology اليوم هنكمل + +7 +00:00:43,020 --> 00:00:48,800 +بجزئية اسمها viral replication انا ابتديت انه نختم + +8 +00:00:48,800 --> 00:00:56,860 +فيها لانههي مدخل لمفاهيم كثيرة صحيح هتزيد من + +9 +00:00:56,860 --> 00:01:00,180 +مداركنا ان شاء الله و قصة منها و اتأهلنا في مساعدة + +10 +00:01:00,180 --> 00:01:04,340 +الفيروسات الى يعني ان نكون عندنا معلومات اساسية + +11 +00:01:04,340 --> 00:01:08,760 +رائعة جدا نبدأ بيها course الفيروسات ايضا بانطلاقة + +12 +00:01:08,760 --> 00:01:13,420 +جيدة ان شاء الله و اذكركم ان الفيروسات كانت عبارة + +13 +00:01:13,420 --> 00:01:17,200 +عن obligate intracellular parasites وبالتالي يعني + +14 +00:01:17,200 --> 00:01:21,290 +they cannot replicate independentlyالمستحيل يعني + +15 +00:01:21,290 --> 00:01:25,250 +يتكاثروا outside كلمة independently يعني بشكل + +16 +00:01:25,250 --> 00:01:30,130 +مستقل وبالتالي they have to have a host to + +17 +00:01:30,130 --> 00:01:33,790 +replicate within ففي بعض الفيروسات بتتاخد + +18 +00:01:33,790 --> 00:01:37,210 +البكتيريا host ومسميها bacteria وفهاش قد تتاخد + +19 +00:01:37,210 --> 00:01:39,930 +النباتات ومسميها plant viruses او قد تتاخد + +20 +00:01:39,930 --> 00:01:43,410 +الحيوانات وبمعنى animal viruses او قد تتاخد + +21 +00:01:43,410 --> 00:01:48,310 +الإنسان ومسميها human virusestheir replication is + +22 +00:01:48,310 --> 00:01:50,570 +not by simple binary vision زي ما روحناها في + +23 +00:01:50,570 --> 00:01:54,130 +البكتيريا ولا mitosis ولا meiosis one virus بدخل + +24 +00:01:54,130 --> 00:01:56,830 +داخل الخلية بيطلع مئات ال virus بيطلع مئات + +25 +00:01:56,830 --> 00:02:02,650 +الفيروسات يبجى هنحاول نطلع على ال growth و ال + +26 +00:02:02,650 --> 00:02:08,630 +multiplication تبع الفيروس بصورتين شكلين العدد مهم + +27 +00:02:10,070 --> 00:02:13,130 +و العيب ممكن برضه ننظر إيه لو من .. فاكرين ال + +28 +00:02:13,130 --> 00:02:19,430 +growth curve تبع البكتيريا؟ لو نظرنا لهذه النظره + +29 +00:02:19,430 --> 00:02:24,270 +هنشوفه بنظره مختلفة شوية عن البكتيريا لكن النظره + +30 +00:02:24,270 --> 00:02:28,810 +اللي احنا هنبصل فيها اكتر هي stepwise خطوة خطوة + +31 +00:02:28,810 --> 00:02:32,890 +كيف الفيروس من ما حد نبيه يجي خارج الخلية ايش معنى + +32 +00:02:32,890 --> 00:02:33,550 +بالتصف في هذه الخلية؟ + +33 +00:02:37,130 --> 00:02:40,270 +الفيروسات كلها تدخل بالنفس الطريقة ولا في اختلافات + +34 +00:02:40,270 --> 00:02:44,030 +لما يدخل ايش بصير ايش كيف .. و اين بتبقى التكارث + +35 +00:02:44,030 --> 00:02:47,030 +في ال site of plasma ولا في النواة؟ ايش نوع المادة + +36 +00:02:47,030 --> 00:02:51,030 +الوراثية للفيروس؟ وهل نوع المادة الوراثية للفيروس + +37 +00:02:51,030 --> 00:02:54,050 +بتأثر على ان يكون ال replication او في ال site of + +38 +00:02:54,050 --> 00:02:58,270 +plasma او في النواة؟ و .. و .. وكنت؟ وما هي ال + +39 +00:02:58,270 --> 00:03:02,530 +replication هتبين معانا ب stepwise يعني خطوة .. + +40 +00:03:02,530 --> 00:03:07,770 +خطوةنطلع الشكل اللي قدامنا هذا الشكل اللي قدامنا + +41 +00:03:07,770 --> 00:03:11,890 +بيبيننا ان هو تجاهز نتكلم عن زمن وعدد هي عندي هنا + +42 +00:03:11,890 --> 00:03:17,470 +زمن صفر ل 12 ساعة وخطرها نتكلم عن hours دخل + +43 +00:03:17,470 --> 00:03:20,870 +الفيروس one particle هذا ال number one particle + +44 +00:03:20,870 --> 00:03:26,170 +بعد ساعة و نص تقريبا اختفى الفيروس لم يعد قابل + +45 +00:03:26,170 --> 00:03:30,490 +لإنه ال malodication ليش؟ لأنه دخل جوا الخلية وما + +46 +00:03:30,490 --> 00:03:36,010 +دخل جوا الخلية هو الآن بطل الفيروس كاملوبالتالي + +47 +00:03:36,010 --> 00:03:42,070 +اختفى عن الوجود بعد اربع ساعات الى خمس ساعات صار + +48 +00:03:42,070 --> 00:03:47,670 +عندي خطين خط المقطع هو المادة الوراثية ممكن على + +49 +00:03:47,670 --> 00:03:52,070 +فكرة نكشف عن كمية المادة الوراثية وممكن نكشف عن + +50 +00:03:52,070 --> 00:03:59,570 +عدد الفيروساتواجبنا ان المادة الوراثية وعدد + +51 +00:03:59,570 --> 00:04:03,450 +الفيروسات ماشيين مع بعض بنفس الوسيلة لكن لاحظوا + +52 +00:04:03,450 --> 00:04:08,010 +فينا logarithmic increase in number برضه في زيادة + +53 +00:04:08,010 --> 00:04:12,410 +عددية مطردة كبيرة بدأنا بالفيروس طلعنا على وين + +54 +00:04:12,410 --> 00:04:17,010 +وصلنا الان للمستوى هنا 100 تقريبا او اكتر من 100 + +55 +00:04:17,010 --> 00:04:22,510 +اكتر من 100 خلال كام ساعةهي من zero ل 12 او ل 10 + +56 +00:04:22,510 --> 00:04:26,810 +ساعات 10 ساعات هي ال maximum هي ال maximum طبعا + +57 +00:04:26,810 --> 00:04:31,630 +هذا من خلية واحدة ليش اثبات هذا؟ لأن مافيش خلايا + +58 +00:04:31,630 --> 00:04:35,290 +اخرى مافيش خلايا اخرى يعملها infection مافيش خلايا + +59 +00:04:35,290 --> 00:04:39,410 +اخرى يعملها .. يعني هو دخل في خلية واحدة و قصناها + +60 +00:04:39,410 --> 00:04:43,470 +لما طلع من نفس الخلية مافي خلايا اخرى لو استمرنا + +61 +00:04:43,470 --> 00:04:47,590 +نوفر للفيروس خلايا هلاجي ان الخط بضل بشكل ايه؟ + +62 +00:04:47,590 --> 00:04:55,760 +مستقيملحد ما يتنافس الخربان مع اللي هو ال + +63 +00:04:55,760 --> 00:04:59,120 +effective او ال infective وبالتالي يعمله + +64 +00:04:59,120 --> 00:05:06,290 +neutralizationتصور عن الفيروس دخل اختفى بعدين فترة + +65 +00:05:06,290 --> 00:05:11,290 +اختفاء بيسموها فترة الكسوف eclipse eclipse period + +66 +00:05:11,290 --> 00:05:17,490 +وفترة الزيادة بيسميها rise اللي هي الصعود صعود نجم + +67 +00:05:17,490 --> 00:05:20,850 +الفيروس في هذا الحالة اذا في اختفاء وفي انهار صعود + +68 +00:05:20,850 --> 00:05:28,760 +الان الفيروس لما دخل الخلية كيف طلع منها؟طلع منها + +69 +00:05:28,760 --> 00:05:34,780 +بسلام خلاها intact و لا ناتت الخلية اتدمر الخلية + +70 +00:05:34,780 --> 00:05:39,500 +هناك بعض الفيروسات عند المقدرة انها تحلل الخلية و + +71 +00:05:39,500 --> 00:05:43,460 +هناك فيروسات بتطلع من الخلية دون ان تؤذيها دون ان + +72 +00:05:43,460 --> 00:05:47,560 +تؤذيها يبجى معناه الحكي ان في فيروسات بتدمر + +73 +00:05:47,560 --> 00:05:53,100 +الخلايا انا بتخيل معايا طبقة من الخلايا نوع من + +74 +00:05:53,100 --> 00:05:57,770 +انواع الخلايا الطلائية لها شكل معروفتحت ال + +75 +00:05:57,770 --> 00:06:02,370 +microscope normal cells و monolayer طبقة واحدة قدأ + +76 +00:06:02,370 --> 00:06:05,510 +حطينا فيها الفيروسات الفيروس حط قدأ أعلى خلية + +77 +00:06:05,510 --> 00:06:09,390 +واحدة دمرها انتقل الخلايا الأخرى دمرها إن أنا صار + +78 +00:06:09,390 --> 00:06:13,590 +في عندي abnormal morphology للخلايا في destruction + +79 +00:06:13,590 --> 00:06:19,750 +في licenseأو الـ Change in the morphology نوع + +80 +00:06:19,750 --> 00:06:23,170 +المورفولوجي بنسميه Cytopathic Effect اللي هو CPE + +81 +00:06:23,170 --> 00:06:28,030 +اللي مذكور قبلنا على الصورة CPE Cytopathic Effect + +82 +00:06:28,030 --> 00:06:32,710 +ليش أنا باهتم فيها Cytopathic Effect؟ لأن كل فيروس + +83 +00:06:32,710 --> 00:06:38,210 +بيعمل Cytopathic Effect خاص فيه كلمة Cytopathic + +84 +00:06:38,210 --> 00:06:39,170 +Effect خاص فيه + +85 +00:06:42,110 --> 00:06:46,770 +في التعرف على مين على الفيروس طبعا كمان مرة اذكركم + +86 +00:06:46,770 --> 00:06:51,010 +not all viruses can produce cytopathic effect لإن + +87 +00:06:51,010 --> 00:06:54,490 +احنا كنا جابا شويه لإن في فيروسات أصلا مابتدمرش + +88 +00:06:54,490 --> 00:06:57,850 +الخلايا وعشان انا أؤكد على الحقيقة هذه بدي أرجع + +89 +00:06:57,850 --> 00:06:58,870 +أوريكم بعض ال animation + +90 +00:07:18,930 --> 00:07:21,850 +الفيروس الان المفروض انه بيعمل عملية attachment + +91 +00:07:21,850 --> 00:07:26,610 +للخلية، بيعمل injection لل DNA تبقى أو كله يدخل + +92 +00:07:26,610 --> 00:07:31,690 +داخل الخلية و بعدين يبدأ عملية تكاتر و تصنيع + +93 +00:07:31,690 --> 00:07:35,070 +بروتينات، منها ما هو structural و منها ما هو + +94 +00:07:35,070 --> 00:07:42,690 +enzymes لغرض تشكيل كامل للفيروس، يعني ان المادة + +95 +00:07:42,690 --> 00:07:44,470 +الوراطية بشكل كبير + +96 +00:07:47,100 --> 00:07:51,780 +معرنيه بيطلع صيد بلازم بيصير له translation بيطلع + +97 +00:07:51,780 --> 00:07:54,200 +ال protein و من ضمنها ال viral protein اللي هي ال + +98 +00:07:54,200 --> 00:07:58,620 +capsid بترجع و بتكون اللي هو ال variant اللي هي + +99 +00:07:58,620 --> 00:08:03,420 +الفيروس الكامل الان بدي اعمل release عمل release + +100 +00:08:03,420 --> 00:08:08,260 +عن طريق ايش destruction لل cell membrane هذا ال + +101 +00:08:08,260 --> 00:08:10,380 +destruction لل cell membrane كمان مرة + +102 +00:08:17,070 --> 00:08:19,810 +انه characteristic يعني مميز لله دعونا نطلع على + +103 +00:08:19,810 --> 00:08:26,330 +animation تاني ونشوف + +104 +00:08:26,330 --> 00:08:29,330 +نوع اخر من ال release لما بيطلع الفيروس من بعد .. + +105 +00:08:29,330 --> 00:08:41,690 +من ال .. من الخلية هذا + +106 +00:08:41,690 --> 00:08:45,290 +عمل ايش لنا؟ هذا عمل ايش اللي شفنا؟ اللي وقع على + +107 +00:08:45,290 --> 00:08:45,570 +طول + +108 +00:08:50,260 --> 00:08:55,580 +هلاج انه الفيروس هو طالع مش هيعمل lysis هياخد جزء + +109 +00:08:55,580 --> 00:08:59,140 +من ال membrane و يطلع فيه هذا سمينا احنا body + +110 +00:08:59,140 --> 00:09:03,540 +عملية اللي هو ال body فمش كل الفيروسات عند المقضرة + +111 +00:09:03,540 --> 00:09:07,990 +تعمل cytopathic effectطبعا هو اكيد فيه ضرر للخلايا + +112 +00:09:07,990 --> 00:09:12,470 +لكن مش مري مش visible انها غير قابل ان احنا نشوفه + +113 +00:09:12,470 --> 00:09:16,650 +بطريقة واضحة واعتقد ان الفيروس هذا او النموذج هذا + +114 +00:09:16,650 --> 00:09:22,070 +اللي قدمه الان هو شكل على فيروس بياخد سيل من plane + +115 +00:09:22,070 --> 00:09:27,970 +بدون ما يعمل destruction للخلايا بس يبدو انه بيعمل + +116 +00:09:27,970 --> 00:09:32,130 +اثارة تشويق الفيروس هذا فبالتالي بياخد وقت اطول في + +117 +00:09:32,130 --> 00:09:33,330 +العالم + +118 +00:09:35,820 --> 00:09:38,700 +هذه ال glycoproteins خاصة بالفيروس الفيروس اللي + +119 +00:09:38,700 --> 00:09:41,300 +بيصدرها نعم هو اللي بيكوّنها و هذا كل ما حكيناه في + +120 +00:09:41,300 --> 00:09:45,080 +المحاضرة + +121 +00:09:45,080 --> 00:09:50,300 +الأولى للفيروس هذا ايوة دلنا ال cell membrane بقى + +122 +00:09:50,300 --> 00:09:56,040 +كمان و الخلية دلت عايشة و دايما ليس من الحكمة ان + +123 +00:09:56,040 --> 00:10:01,120 +ال micro organism يقتل ال host قلنا لكوا الميكروب + +124 +00:10:01,120 --> 00:10:07,480 +الناجح هو اللي بتكاترو بيحدث أقل ضرر ممكن لل hosts + +125 +00:10:07,480 --> 00:10:11,940 +لأن هذا بيضمن اللي هو طالب ال hosts survive هو + +126 +00:10:11,940 --> 00:10:18,000 +survive بينما قدر لل hosts و بعدين راح موت اللي هو + +127 +00:10:18,000 --> 00:10:20,400 +كل ال virgin اللي طلعها ال whites + +128 +00:10:28,790 --> 00:10:32,470 +تفضلنا أن هناك تصورين للـreplication تصور + +129 +00:10:32,470 --> 00:10:35,070 +بالـgrowth curve و تصور tennis stepwise اللي هو + +130 +00:10:35,070 --> 00:10:39,150 +خطوة بخطوة يطلع على المجموع الخطوات الرئيسية في + +131 +00:10:39,150 --> 00:10:43,930 +تصنيع الفيروسة أنا جالس هنا من أتلات مراحل فاكرين + +132 +00:10:45,650 --> 00:10:50,270 +attachment and penetration أو entry, synthesis + +133 +00:10:50,270 --> 00:10:54,890 +وكلمة synthesis كانت كلمة واسعة تحتوي إلى معاملات + +134 +00:10:54,890 --> 00:10:58,730 +كبيرة و في الآخر فين دي assembly و كان هنا release + +135 +00:10:58,730 --> 00:11:01,630 +لكن لاحظوا أن أنا فين دي خطوات أكتر من اللي أنا + +136 +00:11:01,630 --> 00:11:05,910 +حكيتهملأنه بالفعل عملنا عملية ايه؟ تفصيل في عملية + +137 +00:11:05,910 --> 00:11:10,050 +ال synthesis اتكلمنا عن early mRNA و early protein + +138 +00:11:10,050 --> 00:11:13,190 +synthesis وبعدين اتكلمنا عن genome synthesis + +139 +00:11:13,190 --> 00:11:16,470 +وبعدين اتكلمنا عن late اللي هو protein mRNA و + +140 +00:11:16,470 --> 00:11:19,170 +protein synthesis وبعدين اتكلمنا عن ال assembly و + +141 +00:11:19,170 --> 00:11:22,910 +ال release شكل + +142 +00:11:22,910 --> 00:11:27,330 +typical من أشهر ال life cycles المعروفة ال + +143 +00:11:27,330 --> 00:11:33,800 +adenovirus ال adenovirusesطبعا الفيروس على الخلية + +144 +00:11:33,800 --> 00:11:37,600 +الخلية الكبيرة في هذه الدائرة هي الخلية الكبيرة + +145 +00:11:37,600 --> 00:11:40,460 +اللي في النص هي النوية طبعا الان الفيروس عمل + +146 +00:11:40,460 --> 00:11:46,160 +attachment دخل الفيروس عمل uncoating دخل المادة + +147 +00:11:46,160 --> 00:11:49,960 +الأوراطية اللي هي شايفينها دى لوين للنوية ليش دخل + +148 +00:11:49,960 --> 00:11:54,460 +للنوية لأن هو DNA virus DNA virus غالبا ال DNA + +149 +00:11:54,460 --> 00:11:55,440 +virus بتحدم + +150 +00:11:59,870 --> 00:12:03,730 +على النوى أول خطوة في النوى بصيله transcription هي + +151 +00:12:03,730 --> 00:12:07,870 +بتطلع منه mRNA هاي ال mRNA شايفينه هذا هذا mRNA + +152 +00:12:07,870 --> 00:12:12,070 +بعد ال mRNA مايتصنع وان بروح على ال cytoplasm + +153 +00:12:12,070 --> 00:12:16,010 +بتطلع هي شايفينه ايش بايش مرتبط عليه مرتبط عليه ال + +154 +00:12:16,010 --> 00:12:21,570 +ribosome هنا يبقى صار لنا early protein synthesis + +155 +00:12:21,570 --> 00:12:26,330 +يبقى صار فاندي mRNA هي شايفينه early mRNA و بعدين + +156 +00:12:26,330 --> 00:12:26,750 +early + +157 +00:12:33,160 --> 00:12:38,320 +لأن البروتين تصنعت في الـ cytoplasm + +158 +00:12:41,110 --> 00:12:44,570 +ده اغلب النواة هي ده اغلب النواة يبجى معنا الحاجة + +159 +00:12:44,570 --> 00:12:48,950 +ان ال early proteins لها دور رئيسي في تصنيع ال + +160 +00:12:48,950 --> 00:12:53,210 +late protein و تصنيع الليبروجيني اللي هو المادة + +161 +00:12:53,210 --> 00:12:58,210 +الورادية اللي هتشكل النسل دبع هذا الفيروس و بنكمل + +162 +00:12:58,210 --> 00:13:02,050 +السلسلة تصنية و تصنية و تصنية و بالاخر وسلسلة اللي + +163 +00:13:02,050 --> 00:13:07,010 +هو Tetrisإذن باختصار شديد جدا Attachment, + +164 +00:13:07,170 --> 00:13:10,290 +Penetration, Uncoating, Early Synthesis للـ mRNA, + +165 +00:13:10,490 --> 00:13:13,710 +Early Synthesis للبروتين, Progeny DNA أو نوكليك + +166 +00:13:13,710 --> 00:13:17,690 +أسد, بعدين Late Synthesis للـ mRNA, Late Synthesis + +167 +00:13:17,690 --> 00:13:21,310 +للبروتين, بعدين Assembly، بعدين Release نبدأ من + +168 +00:13:21,310 --> 00:13:25,630 +الآخر، من الآخر انتشرتوا اللي من الآخر، من الآخر + +169 +00:13:25,630 --> 00:13:29,350 +انتشرتوا قبل دقائق ال release ال release كان إن + +170 +00:13:29,350 --> 00:13:34,400 +عبر ال lysisاللي هو ال budding عبر ال budding اللي + +171 +00:13:34,400 --> 00:13:37,760 +بيعمل license غالبا هذي استراتيجية من استراتيجيات + +172 +00:13:37,760 --> 00:13:43,920 +الفيروسات التي بتحتوي على envelope بينما ال + +173 +00:13:43,920 --> 00:13:47,600 +budding هو استراتيجية من استراتيجيات الفيروسات + +174 +00:13:47,600 --> 00:13:52,860 +اللي لها ل envelope طب ال assembly كيف بيصير ال + +175 +00:13:52,860 --> 00:13:59,460 +assembly بيصير بطريقة نحن لم نفهمها بعد يعني + +176 +00:13:59,460 --> 00:14:04,600 +ماعناش معلومات كافية عنهااللي بنعرفه حاجة واحدة + +177 +00:14:04,600 --> 00:14:07,920 +ذكرناها في المحاضرة السابقة ال self-assembly + +178 +00:14:07,920 --> 00:14:11,900 +فاكرينها؟ و نقول لما جيبنا purified protein و + +179 +00:14:11,900 --> 00:14:15,700 +جيبنا mRNA تبع الفيروس و حطناهم في test tube + +180 +00:14:15,700 --> 00:14:22,770 +فاجأنا لحالهم انهم ايه؟ و هذا جونا عمليعني قريب من + +181 +00:14:22,770 --> 00:14:26,870 +المغناطيسات مثلا يعني لو جيبت انا مجموعة مغناطيسات + +182 +00:14:26,870 --> 00:14:29,950 +احلتهم مع بعض بين اللي جيهم اخدوا شكل حالهم من غير + +183 +00:14:29,950 --> 00:14:32,190 +ما نبدل اي جهد يلزجوا مع بعض لا بدنا اللي لزجوا + +184 +00:14:32,190 --> 00:14:36,020 +ولا غير و لزجوا مع بعضيجي المشكلة أو خدنا نقول + +185 +00:14:36,020 --> 00:14:40,620 +التفسير الأقرب إلى انه .. نقول انه تركيب ال + +186 +00:14:40,620 --> 00:14:43,640 +protein و المادة الوراقية في بينهم interaction .. + +187 +00:14:43,640 --> 00:14:49,480 +interaction تلقائي لا يستلزم اي نوع من التدخل اللي + +188 +00:14:49,480 --> 00:14:54,010 +بتطاقةخلية مش هتصرف طاقة على ال assembly ال + +189 +00:14:54,010 --> 00:14:59,450 +assembly عملية غالب الأمر self motivated ناتجة عن + +190 +00:14:59,450 --> 00:15:02,910 +مين؟ ناتجة عن ال nature of the structure of + +191 +00:15:02,910 --> 00:15:05,850 +protein and nucleic acid بتفاعلوا مع بعض و + +192 +00:15:05,850 --> 00:15:09,750 +بيرتبطوا بطريقة نظامية معينة أما كده بالضبط ماعنا + +193 +00:15:09,750 --> 00:15:13,670 +فكرة الصحيح خلاصها من ال assembly ال assembly + +194 +00:15:13,670 --> 00:15:19,300 +غالبا بتمن .. غالبا بتمنفي الخلية وبعدين و الفيروس + +195 +00:15:19,300 --> 00:15:22,120 +طالع خلاص يعني هي بيكون already جاهز في بعض + +196 +00:15:22,120 --> 00:15:26,180 +الفيروسات ال assembly بتتم في أخر لحظات قبل خروجه + +197 +00:15:26,180 --> 00:15:30,820 +بلحظات شفت ال budding؟ ال budding عند عريض ال + +198 +00:15:30,820 --> 00:15:34,520 +budding بيصير في اللي هو ال assembly الأخيرة و + +199 +00:15:34,520 --> 00:15:37,100 +بيطلع يعني ال maturation للفيروس ال maturation + +200 +00:15:37,100 --> 00:15:41,320 +للفيروس طب نرجع للخطوة اللي جابلها كان في عيني أنا + +201 +00:15:41,320 --> 00:15:44,770 +protein synthesis صح؟ و ده protein synthesisخدوا + +202 +00:15:44,770 --> 00:15:49,050 +بالك ال protein synthase ممكن يتم بطريقتين عندي + +203 +00:15:49,050 --> 00:15:52,490 +خيط طويل من ال mRNA بيجرى البزوم بيجرى من أول و + +204 +00:15:52,490 --> 00:15:57,870 +لآخر بيطلع عنده long polypeptide مايله أي وظيفة + +205 +00:15:57,870 --> 00:16:02,710 +long polypeptide مالوش أي وظيفة بالضبط أنا بشبهها + +206 +00:16:02,710 --> 00:16:06,370 +قطعة الخشب عند النجار بتكون محتوطة مايلها غرض و + +207 +00:16:06,370 --> 00:16:11,250 +ليها مسمة و ليها وظيفة قطعة خشب فجأة بيبرسم عليها + +208 +00:16:11,250 --> 00:16:15,120 +و بجسها بالمنشفة اللي صارت طاولةصح لها function صح + +209 +00:16:15,120 --> 00:16:19,320 +لها في هذه الحالة functional على طرف ال + +210 +00:16:19,320 --> 00:16:23,620 +polypeptide بيكون قطعة بيسموها protease انزين + +211 +00:16:23,620 --> 00:16:28,080 +protease تفصل حالها من حالها من القطع هذه و بتبدأ + +212 +00:16:28,080 --> 00:16:33,100 +تجتف ال polypeptide من أماكن محددة طبعا كل قطعة + +213 +00:16:33,100 --> 00:16:37,620 +تنتج بيسميها functional functional polypeptide + +214 +00:16:39,250 --> 00:16:42,610 +أو functional protein نشرت من ايه؟ من قطعة طويلة + +215 +00:16:42,610 --> 00:16:45,130 +بيسموها precursor polypeptide precursor + +216 +00:16:45,130 --> 00:16:50,650 +polypeptide إذا هناك فيروسات عندها استراتيجية ان + +217 +00:16:50,650 --> 00:16:55,130 +تصنع قطعة طويلة من ال polypeptide سمنها precursor + +218 +00:16:55,130 --> 00:16:59,690 +وبعدها أنزيم البروتياز هو اللي بيجتح ناخد فائدة من + +219 +00:16:59,690 --> 00:17:06,330 +هذا الموضوعالبروتيايز بدونه .. اه بدونه الفيروس مش + +220 +00:17:06,330 --> 00:17:09,290 +هيقدر يكمل ال life cycle تبعته لأنه معتمد اعتماد + +221 +00:17:09,290 --> 00:17:12,090 +كامل عالمين على ال protein انه يتقطع و يصبح + +222 +00:17:12,090 --> 00:17:17,390 +functional فلو استخدمنا احنا drugs that will + +223 +00:17:17,390 --> 00:17:20,330 +inhibit the انزيم اللي اسمه البروتيايز وبسموه + +224 +00:17:20,330 --> 00:17:24,740 +بالفعل البروتيايز inhibitorsممكن نستهدف تلك + +225 +00:17:24,740 --> 00:17:30,260 +الفيروسات اللتي .. اللتي من استراتيجيتها ان تصنع + +226 +00:17:30,260 --> 00:17:33,580 +.. تصنع اللي هو قلنا الـ long poly peptide و من + +227 +00:17:33,580 --> 00:17:37,980 +أشهر الفيروسات كرمولة اللي بنستخدمها Anti Protease + +228 +00:17:37,980 --> 00:17:41,300 +Inhibitors او Protease Inhibitors اللي هي الـ HIV + +229 +00:17:41,300 --> 00:17:47,780 +الـ HIV وصلت النقطة و هناك طبعا الفيروسات + +230 +00:17:54,800 --> 00:17:59,380 +بينما النوع الآخر من الفيروسات النوع الآخر من + +231 +00:17:59,380 --> 00:18:03,820 +الفيروسات أصلا عنده المادة الوراثية مقطعة زي ال + +232 +00:18:03,820 --> 00:18:08,280 +influenza virus ال influenza virus فكل قطعة بصي + +233 +00:18:08,280 --> 00:18:13,740 +لها transcription و بيمر عليها ال translation و + +234 +00:18:13,740 --> 00:18:16,460 +بتاني بتنتج functional protein ككل قطعة بتنتج + +235 +00:18:16,460 --> 00:18:19,560 +functional protein يبقى في عندنا two strategies + +236 +00:18:19,560 --> 00:18:25,440 +كمان مرة واحدة و بشرة انه نعملهاfunctional protein + +237 +00:18:25,440 --> 00:18:29,420 +و واحدة precursor polypeptide من خلالها بنصنع + +238 +00:18:29,420 --> 00:18:35,160 +مجموعة من البروتينات مرة واحدة طب هذا متعلق بمين؟ + +239 +00:18:35,160 --> 00:18:41,320 +متعلق بال polypeptide و ال protein synthesis طب + +240 +00:18:41,320 --> 00:18:43,880 +احنا جاب ال protein synthesis فيها مشكلة كبيرة + +241 +00:18:43,880 --> 00:18:48,060 +اللي اهم من كل هذه تلقى عليك انت الطريقة اللي + +242 +00:18:48,060 --> 00:18:52,400 +بيجيه من أي بواية بتيجيه؟من البوارة الغربية + +243 +00:18:52,400 --> 00:18:57,020 +الشرقية وفيه بيجوا من البوارة الجنوبية وفي بعض + +244 +00:18:57,020 --> 00:18:59,700 +الأحيان لك لكن كلهم وان بيروحوا في الآخر بيجوا على + +245 +00:18:59,700 --> 00:19:03,840 +القاعة بيجوا على القاعة دخلوا من أبواب مختلفة + +246 +00:19:03,840 --> 00:19:06,260 +بطولة مختلفة لكن في النهاية دخلوا من الأساس + +247 +00:19:06,260 --> 00:19:11,610 +المسلطة احنا الفيروسات تشكلتالمادة اللي قاعدة + +248 +00:19:11,610 --> 00:19:14,650 +تبعتها بطرق مختلفة فيه positive-stranded DNA فيه + +249 +00:19:14,650 --> 00:19:17,830 +single-stranded DNA فيه positive polarity single + +250 +00:19:17,830 --> 00:19:21,510 +-stranded وفيه negative polarity single-stranded + +251 +00:19:21,510 --> 00:19:24,630 +RNA وفيه double-stranded أساسا RNA يعني + +252 +00:19:24,630 --> 00:19:28,150 +استراتيجيات مختلفة في المادة الوراثية + +253 +00:19:42,890 --> 00:19:46,870 +هذه هي الـ ultimate، ال goal + +254 +00:19:52,030 --> 00:19:57,330 +هو يترجم عشان يترجم لازم يتحول ل mRNA طبعا هناك + +255 +00:19:57,330 --> 00:20:02,130 +فيروسات سهل جدا واصلا طبيعتها هي جاية positive + +256 +00:20:02,130 --> 00:20:06,330 +sense positive sense mRNA بتقول الريبوزوم تعال + +257 +00:20:06,330 --> 00:20:06,590 +أقرأ + +258 +00:20:12,550 --> 00:20:17,430 +هل احنا بطبيعة الخلايا تبعتنا فهي لنا إمكانية + +259 +00:20:17,430 --> 00:20:20,390 +نحوّل ال negative ل positive خلايا ناحنا ال host + +260 +00:20:20,390 --> 00:20:24,830 +يعني الفيروس بتكون جايب مع إيه؟ ال enzyme .. ال + +261 +00:20:24,830 --> 00:20:28,730 +enzyme تبعه .. ال enzyme تبعه هنا يجب أن نتوقف و + +262 +00:20:28,730 --> 00:20:35,930 +نتحدث عن concept مهم جدا الفيروس بيحتاج أن يكون + +263 +00:20:35,930 --> 00:20:42,120 +معه enzyme خاص فيه و فيروس آخر لا يحتاجالتنان + +264 +00:20:42,120 --> 00:20:47,060 +حطيتهم في .. في انبوبتان و غسلتهم شيلت منهم + +265 +00:20:47,060 --> 00:20:51,840 +الكبسيد خالص و شيلت منهم أي بروتين ايش اللي طلع + +266 +00:20:51,840 --> 00:20:56,620 +معايا فقط نوكليك .. النوكليك اسن انا اللي قولنا + +267 +00:20:56,620 --> 00:20:59,800 +عنه ب enzyme هذا اظن انه اه اه و بتاني بدوش enzyme + +268 +00:20:59,800 --> 00:21:04,420 +اللي انا أخدتهم أخدت مادة بلاتية فقط فضل حطهم على + +269 +00:21:04,420 --> 00:21:08,680 +الخلايا تفاجأنا ان هذا الفيروس عمل infection + +270 +00:21:08,680 --> 00:21:14,370 +للخلايا و طلعنا فيروسهاوهذا فاش أطلقنا عليه لقب، + +271 +00:21:14,370 --> 00:21:18,810 +بيقول إن هذا اسمه infectious nucleic acid وهذا non + +272 +00:21:18,810 --> 00:21:23,730 +-infectious قاعدة كل فيروس يحتاج إن الانزيم تبع + +273 +00:21:23,730 --> 00:21:27,750 +يكون معاه فهو non-infectious بينما كل فيروس + +274 +00:21:27,750 --> 00:21:32,710 +مابيحتاج إن زهر يكون معاه فهو قادر أن يعمل + +275 +00:21:32,710 --> 00:21:37,630 +infectious طب تعالي ناخد حكمة من الموضوعمين اللي + +276 +00:21:37,630 --> 00:21:42,530 +كان سبب في إنه الفيروس يصيب X وما يصيبش Y؟ وجود + +277 +00:21:42,530 --> 00:21:45,570 +الـReceptors؟ لأ، أتكلم عن ال .. عن الفيروس بشكل + +278 +00:21:45,570 --> 00:21:47,750 +عام، اللي أنا خلصت سنة من الفكرة هذه، رجعنا + +279 +00:21:47,750 --> 00:21:53,290 +للفيروس الأب، العادى، من المسئول عن إنه يصيب X وما + +280 +00:21:53,290 --> 00:21:56,530 +يصيبش Y هو الـReceptors و الـAnti-Radين المولودة + +281 +00:21:56,530 --> 00:21:59,730 +عليه؟ طب لما أنا أخدت المادة الوراطية، المادة + +282 +00:21:59,730 --> 00:22:04,990 +الوراطية إيها الـReceptors؟ بينجأ أنا تخطيت حاجز + +283 +00:22:04,990 --> 00:22:10,200 +اسمه Specificityصح هذا لأ بجد بالأول مضطر ان عشان + +284 +00:22:10,200 --> 00:22:13,700 +هذا الفيروس يدخل داخل الخلية ايش اعمل؟ احط لازم + +285 +00:22:13,700 --> 00:22:16,860 +يكون له receptor طب هذا الان انا بتكلم عن مادة + +286 +00:22:16,860 --> 00:22:20,120 +وراثية مش محتاجة receptor هتدخل الى داخل الخلية + +287 +00:22:20,120 --> 00:22:26,920 +smoothly وانتهى الموضوع لكن سؤال دخلناه داخل + +288 +00:22:26,920 --> 00:22:33,240 +الخلية وتكاتر الفيروس لكن خدوا بالكم الفيروس دمر + +289 +00:22:33,240 --> 00:22:37,830 +الخلية اللي اتكاتر فيها والان طلع على خلايا أخرىهل + +290 +00:22:37,830 --> 00:22:41,590 +بيقدر يعملها infection؟ اه بيقدر يعملها في حالة + +291 +00:22:41,590 --> 00:22:45,210 +واحدة انه .. انه لها receptor بس إذا من هات ال + +292 +00:22:45,210 --> 00:22:50,730 +receptor .. يعني أعطيكم مثال صارخ و بقب أسماء، + +293 +00:22:50,730 --> 00:22:54,730 +فيروس ال poliovirus .. ال poliovirus بصيب مثلا + +294 +00:22:54,730 --> 00:22:58,950 +الإنسان و ال .. ال .. و بعض المخلوقات الشبيهة اللي + +295 +00:22:58,950 --> 00:23:01,790 +زي القرد يعني ال higher .. يسموه higher animals، + +296 +00:23:01,790 --> 00:23:06,690 +اتبعين؟ لكن مابصدش الزواحفلو أنا أخدت ال virus تبع + +297 +00:23:06,690 --> 00:23:10,390 +العفن، المادة الوراثية و حقنتها بالزواحف، هيعمل + +298 +00:23:10,390 --> 00:23:15,110 +infection، لكن الخلية اللي هي حقنتها فيها، هيطلع + +299 +00:23:15,110 --> 00:23:18,250 +ال virus مش هيجي خلايا أخرى يقدر يصيبها، لكن في + +300 +00:23:18,250 --> 00:23:22,350 +هذه الفكرة أنه ثبت أنه نقدر نتخطى حاجز ال + +301 +00:23:22,350 --> 00:23:27,490 +specificity في هذا الحد، في هذه المعنىنرجع نبرجها + +302 +00:23:27,490 --> 00:23:31,190 +ايش اللي خلنا نتكلم عنها انفكشاس و non-infectious + +303 +00:23:31,190 --> 00:23:37,110 +طيب الفيروس اللي ال DNA double-stranded و single + +304 +00:23:37,110 --> 00:23:41,070 +-stranded positive او single-stranded negative كيف + +305 +00:23:41,070 --> 00:23:44,650 +كل المدن اللي بتصرفوا داخل الخلايا هنحاول نتعرف + +306 +00:23:44,650 --> 00:23:48,050 +عليهم عنهم لكن لأ بقصة من المرور سريع الكلام اللي + +307 +00:23:48,050 --> 00:23:53,810 +حاكينها ال attachment هي اللي بتحددspecificity و + +308 +00:23:53,810 --> 00:23:57,430 +ال host range يصيب مين و كيف و حتى ال organ + +309 +00:23:57,430 --> 00:24:02,330 +specificity اللي ذاكرة قالها هذه مهمة جدا لإنه + +310 +00:24:02,330 --> 00:24:09,010 +بنلاقي إنه liver viruses زي مثلا hepatitis viruses + +311 +00:24:10,170 --> 00:24:14,590 +هي سميت hepatitis virus مش لإنها عيلة واحدة، هم من + +312 +00:24:14,590 --> 00:24:18,670 +عائلات مختلفة، لكن ال primary target تبعهم هو ال + +313 +00:24:18,670 --> 00:24:23,270 +liver cells، يعني hepatitis A مختلفة عن B عن C عن + +314 +00:24:23,270 --> 00:24:27,030 +D، كل المختلفين عن بعض، لكن الشيء المشترك بينهم + +315 +00:24:27,030 --> 00:24:30,450 +إنهم بيستخدفوا ال liver cells، يبقى organ + +316 +00:24:30,450 --> 00:24:32,650 +specificity في هذه الحالة + +317 +00:24:35,140 --> 00:24:40,180 +هل لما الفيروس بيرتبط على ال receptor عنا، هل هذا + +318 +00:24:40,180 --> 00:24:44,880 +ال receptor مخصص، مصموة، عشان يستقبل الفيروس؟ لأ، + +319 +00:24:44,880 --> 00:24:48,260 +مش معقول، بس اللي معقول، طبعا، فهذه مثلة، مثلة + +320 +00:24:48,260 --> 00:24:52,200 +خلافية، هتناقش فيها يوم من الأيام، لكن اليوم بدنا + +321 +00:24:52,200 --> 00:24:55,920 +نعرف أنه، احنا بدنا نعرف أنه في بعض الفيروسات + +322 +00:24:55,920 --> 00:25:00,300 +بترتبط ب receptors، هذي ال receptors اللي لها non + +323 +00:25:00,300 --> 00:25:05,820 +-function، يعني لوظيفة معروفة، مثلاالـ CD4 اللى + +324 +00:25:05,820 --> 00:25:10,040 +بيرتبط عليها اللى هو الـ HIV هي ال normal function + +325 +00:25:10,040 --> 00:25:14,820 +تبعتها انها ترتبط ل class II MHC و class II MHC + +326 +00:25:14,820 --> 00:25:19,020 +بروتين مهم جدا فى عملية تنشيط الـ T-Helper cell + +327 +00:25:19,020 --> 00:25:22,540 +يجه هو مش مصمم بالفيروس بس انا بقوله كيف أصيب + +328 +00:25:22,540 --> 00:25:28,680 +الموضوع انا الغرفه يادي المفتاح عشان ندخل + +329 +00:25:34,320 --> 00:25:38,320 +تمام؟ يبقى فيه Normal Function للـ Receptors لكن + +330 +00:25:38,320 --> 00:25:43,260 +الفيروسات استطاعت أن تمتلك مفاتيح للارتباط بهذه + +331 +00:25:43,260 --> 00:25:47,240 +الـ Receptors وعن طريقها استطاعت أن تستهدف طبعا هل + +332 +00:25:47,240 --> 00:25:50,500 +هذا متعمد ولا غير متعمد؟ هذا في علم الغيبر، ربنا + +333 +00:25:50,500 --> 00:25:53,460 +سبحانه وتعالى هو اللي خلقه للمخلوقات ويا ذالري إن + +334 +00:25:53,460 --> 00:25:57,860 +كان هناك حكمة، أكيد حكمة، هناك حكمة، لكن لا العلم + +335 +00:25:57,860 --> 00:26:02,040 +هنا ال Rays Virus بيرتبط بالـ Acetylcholine + +336 +00:26:02,040 --> 00:26:05,680 +Receptorأصدار كلين Receptors عشان هيك تبصير فيانا + +337 +00:26:05,680 --> 00:26:10,120 +أمراض متعلقة بالعصب في ال .. ده الكلام ال Epstein + +338 +00:26:10,120 --> 00:26:12,040 +-Barr أو ال Epstein-Barr Virus بيرتبط ل ال + +339 +00:26:12,040 --> 00:26:16,400 +complement receptor ال Vaccinia virus بيرتبط بحدث + +340 +00:26:16,400 --> 00:26:18,760 +يسمى epidermal growth factor receptor + +341 +00:26:21,790 --> 00:26:24,890 +وهو الـ M1 اللي هو Intracellular Adhesin Molecule + +342 +00:26:24,890 --> 00:26:29,710 +وهذا برضه عبارة معروف باسم CD وكلمة CD معرفت مرة + +343 +00:26:29,710 --> 00:26:32,610 +معاهم قبل البداية CD معناها cluster of + +344 +00:26:32,610 --> 00:26:35,630 +differentiation وهو عبارة عن أنتيجين بيستخدموه + +345 +00:26:35,630 --> 00:26:39,170 +لتمييز بين خلايا ال white blood cell + +346 +00:26:43,480 --> 00:26:46,160 +بالنسبة للهد الأتاسون، بالنسبة للـ Penetration، + +347 +00:26:46,160 --> 00:26:49,960 +فكرين المحاضرة السابقة كانت تتكلم عن Indocytosis + +348 +00:26:49,960 --> 00:26:54,400 +أو عن Fusion، تمام عن Fusion، وبعض الأحيان فقط + +349 +00:26:54,400 --> 00:27:01,260 +Injection للمادة الوراتية الـ Uncoating جوا هنا من + +350 +00:27:01,260 --> 00:27:05,680 +ال pH المنخفض لل physical اللي فيها الفيروس، + +351 +00:27:05,680 --> 00:27:13,100 +favors Uncoatingبتستغربوا احنا كنا نقول انه يعني + +352 +00:27:13,100 --> 00:27:18,540 +مثلا في أشياء زي الأجار بتكون صلبة بنسخنها تسعة + +353 +00:27:18,540 --> 00:27:21,720 +وتمانية وتسعين بتكون لسه صلبة تسعة وتسعين بتصير + +354 +00:27:21,720 --> 00:27:26,740 +سائلة، مظبوط؟ درجة حرارة واحدة بتغير من طبيعات + +355 +00:27:26,740 --> 00:27:33,060 +المركبفال bonding تبع ال capsomers المكونة للكبسيد + +356 +00:27:33,060 --> 00:27:37,280 +بقولنا بال pH المنخفض يعني بتتفكك، الروابط هذه + +357 +00:27:37,280 --> 00:27:41,200 +اللي هي الضعيفة، الروابط ضعيفة تتفكك وبالتالي بـ + +358 +00:27:41,200 --> 00:27:44,000 +circuit release للمادة إيه والعادي، إذا ال low pH + +359 +00:27:44,000 --> 00:27:49,920 +في ال physical favors عن كوترال bacteriophage طبعا + +360 +00:27:49,920 --> 00:27:54,040 +فاكرينه اه بيعمل عملية injection و إذا إنتوا + +361 +00:27:54,040 --> 00:27:59,180 +بتشوفوا أجاز من أروع ما يكون دور على اليوتيوب على + +362 +00:27:59,180 --> 00:28:04,420 +ال penetration لل bacteriophage لل bacterial cell + +363 +00:28:04,420 --> 00:28:08,460 +بتشوفوا ال contracting sheet تمام كيف بتعمل + +364 +00:28:08,460 --> 00:28:12,140 +injection للمادة اللي ورا دى كيف ال base اللي + +365 +00:28:12,140 --> 00:28:15,820 +موجودة في ال core هنا في القطع هذه شايفينهابتحتوي + +366 +00:28:15,820 --> 00:28:21,300 +اصلا على انظمة ما اللي عيزنا نسميها انجيكشن فال .. + +367 +00:28:21,300 --> 00:28:25,300 +ال .. ال .. ال replication للبكتيريا اللي فشفناها + +368 +00:28:25,300 --> 00:28:28,640 +احنا فاكتر من المناسبة و ممكن نركزنا عليه اكتر + +369 +00:28:28,640 --> 00:28:32,420 +حاليا لما تكلمنا عن ال transduction ايه طبعا + +370 +00:28:32,420 --> 00:28:37,780 +animated GIF كرتون يعني بيوضح لنا بشكل مبسط بصرية + +371 +00:28:37,780 --> 00:28:41,920 +كيفية اللي هو الدخول الى البكتيريا لحظه انه + +372 +00:28:44,650 --> 00:28:48,230 +في احتكاك مع الـ cell wall في انجيكشن للمادة + +373 +00:28:48,230 --> 00:28:52,990 +الوراثية في سيطرة كاملة على الخلية وتصنيع ومن ثم + +374 +00:28:52,990 --> 00:28:59,090 +عملية تجميع و release by light هذا نوع من ال light + +375 +00:28:59,090 --> 00:29:03,550 +effect إذا + +376 +00:29:03,550 --> 00:29:05,370 +نقدر نقول إنها غلطية للجزء الأول اللي هو + +377 +00:29:05,370 --> 00:29:07,910 +الattachment و ال penetration و ال uncoating كلها + +378 +00:29:07,910 --> 00:29:10,290 +لأنها عن ال early viral mRNA synthesis و ال early + +379 +00:29:10,290 --> 00:29:11,630 +protein synthesis + +380 +00:29:14,360 --> 00:29:20,140 +في عاملين مهمين جدا بيأثروا على رؤيتنا لكيفية ال + +381 +00:29:20,140 --> 00:29:23,380 +replication العامل الأول المادة الوراثية ايش + +382 +00:29:23,380 --> 00:29:28,660 +طبيعتها؟ رقم اتنين اين يتكاثر؟ في النواء ولا في + +383 +00:29:28,660 --> 00:29:34,520 +الـcytoplasm اكتر صفحة محتوية على معلومات بدنا + +384 +00:29:34,520 --> 00:29:38,620 +نبقى مركزين عليها طول الوقت تمام؟ ونشوف ايش الفرق + +385 +00:29:38,620 --> 00:29:44,930 +ما بين ال DNA virus و ال RNA virusلحظوا كلهم ال + +386 +00:29:44,930 --> 00:29:49,450 +DNA viruses بيتكونوا من double-stranded DNA معدى + +387 +00:29:49,450 --> 00:29:53,310 +ال barbo virus يبقى في exception واحد اسمه barbo + +388 +00:29:53,310 --> 00:29:59,430 +virus هو single-stranded DNA they all replicate in + +389 +00:29:59,430 --> 00:30:06,610 +the nucleus معدى ال box viruses يبقى كلهم بتكاثروا + +390 +00:30:06,610 --> 00:30:10,390 +في النواء معدى ال box virus اللي هو الجملة الأخيرة + +391 +00:30:11,280 --> 00:30:15,360 +They use + +392 +00:30:15,360 --> 00:30:20,160 +a host cell DNA dependent RNA polymerase to + +393 +00:30:20,160 --> 00:30:24,080 +synthesize their mRNA. Are they infectious or non + +394 +00:30:24,080 --> 00:30:24,940 +-infectious؟ + +395 +00:30:28,300 --> 00:30:33,360 +ال DNA تبعهم لأ اللي جابل ال DNA Virus اللي حتى + +396 +00:30:33,360 --> 00:30:36,460 +اللي بتكلم عنهم اللي هم بيستخدموا ال DNA dependent + +397 +00:30:36,460 --> 00:30:39,740 +RNA Polymerase لبعض الخلية لإن هو بيستخدموش معاهم + +398 +00:30:39,740 --> 00:30:45,520 +اللي هو ال انزمات تبعه بيستغلوا ال hostbox viruses + +399 +00:30:45,520 --> 00:30:48,320 +are the exception because they replicate in the + +400 +00:30:48,320 --> 00:30:52,660 +cytoplasm where they do not have access to the + +401 +00:30:52,660 --> 00:30:56,120 +host cell RNA polymerase بجه هذا مضطر يجيب الانزين + +402 +00:30:56,120 --> 00:31:00,600 +تبعه معاه اللي هو ال box virus وبتالي ما يمكنش نجي + +403 +00:31:00,600 --> 00:31:04,360 +لل RNA virus ع السريع هدا كلهم double stranded + +404 +00:31:04,360 --> 00:31:07,000 +هدولة single stranded وطبيعته أصلا أن يكون single + +405 +00:31:07,000 --> 00:31:11,220 +strand ال RNA لكن في exceptions أكتر من اللي هو ال + +406 +00:31:12,650 --> 00:31:16,230 +Except for members of the Rio virus family which + +407 +00:31:16,230 --> 00:31:22,950 +have a double-stranded RNA من + +408 +00:31:22,950 --> 00:31:30,930 +الريو فايروسز + +409 +00:31:30,930 --> 00:31:35,790 +في نموذج مشهور جدا اسمه rotavirus + +410 +00:31:41,020 --> 00:31:44,220 +الثالثة مرتبط بالفيكال اورال ترانزميشن فيكال اورال + +411 +00:31:44,220 --> 00:31:52,180 +ترانزميشن فيكال + +412 +00:31:52,180 --> 00:31:55,360 +اورال ترانزميشن فيكال اورال ترانزميشن فيكال اورال + +413 +00:31:55,360 --> 00:31:56,380 +ترانزميشن فيكال اورال ترانزميشن فيكال اورال + +414 +00:31:56,380 --> 00:31:58,020 +ترانزميشن فيكال اورال ترانزميشن فيكال اورال + +415 +00:31:58,020 --> 00:31:58,800 +ترانزميشن فيكال اورال ترانزميشن فيكال اورال + +416 +00:31:58,800 --> 00:32:00,720 +ترانزميشن فيكال اورال ترانزميشن فيكال اورال + +417 +00:32:00,720 --> 00:32:04,700 +ترانزميشن فيكال اورال ترانزميشن فيكال اورال + +418 +00:32:04,700 --> 00:32:09,960 +ترانزميشن فيكالالـ hepatitis D defective هو التالت + +419 +00:32:09,960 --> 00:32:11,560 +اللي احنا حاولنا ان نحكي عنه يبقى فيه اتنين رئيسين + +420 +00:32:11,560 --> 00:32:15,940 +و واحد تالت هدولة ايش مالهم؟ اللي هم خطوات تكاثر + +421 +00:32:15,940 --> 00:32:20,720 +في وين؟ في النواء .. في النواء .. انا اتكلم عن ال + +422 +00:32:20,720 --> 00:32:29,670 +RNA virus مثلا عندنا ال retro virusretrovirus + +423 +00:32:29,670 --> 00:32:36,910 +integrate a DNA copy بيدمش قطعة من ال .. او نسخة + +424 +00:32:36,910 --> 00:32:40,690 +من ال DNA تبعه فى ال hosts ال chromosome او الcell + +425 +00:32:40,690 --> 00:32:43,970 +دى بيجوانوا بحكومهم على الكلام ان هو اكيد فى ان هو + +426 +00:32:43,970 --> 00:32:49,370 +influenza virus synthesizes their progeny نسلهم + +427 +00:32:49,370 --> 00:32:53,390 +genome in the nucleus والاخير بيقولنا اللى هو + +428 +00:32:56,270 --> 00:32:59,770 +بتقولنا كمان إيه الخطوات في اللي هو ال nucleus إذا + +429 +00:32:59,770 --> 00:33:02,630 +لو حد على فكرة فيها كتير معلومات فيها بتقولنا إن + +430 +00:33:02,630 --> 00:33:09,550 +كلهم double-stranded ما عدا ال barbovirus كلهم + +431 +00:33:09,550 --> 00:33:12,950 +في النواء ما عدا ال box virus التانين ال RNA virus + +432 +00:33:12,950 --> 00:33:17,350 +كلهم single-stranded ما عدا الrio virus كلهم في + +433 +00:33:17,350 --> 00:33:20,870 +الcytoplasm ما عدا تلاتة اللي هم ال retrox و ال + +434 +00:33:20,870 --> 00:33:26,190 +influenza زاد اللي هو ال hepatitis deltaمهمة جدا + +435 +00:33:26,190 --> 00:33:30,410 +اللي هو العملية طيب تلعبوا على المنظر هذا بس و + +436 +00:33:30,410 --> 00:33:35,690 +اعرفوا الأنواع double stranded viruses + +437 +00:33:35,690 --> 00:33:40,770 +و انا راح على طول على النواب inborn + +438 +00:33:40,770 --> 00:33:43,510 +and able بدل وعصر تبلازم صاروا transcription early + +439 +00:33:43,510 --> 00:33:47,470 +البروتينات اللي بتصنع ترجع على النواب بتساعد في + +440 +00:33:47,470 --> 00:33:51,330 +اللي هو تصنيع المادة الوراثية و كمان replication و + +441 +00:33:51,330 --> 00:33:56,070 +transcription مرة أخرى لل late proteinsوانا صار + +442 +00:33:56,070 --> 00:34:00,470 +زحمة في الخلية الصحية صار شغل كتير يعني فال early + +443 +00:34:00,470 --> 00:34:03,730 +.. ال early .. انا زي ما بقول .. بقول مثل نموذج + +444 +00:34:03,730 --> 00:34:08,290 +لما الواحد بيجيب يكتب ورقة او كتاب بيجيب كتب برضه + +445 +00:34:08,290 --> 00:34:12,770 +فالكتاب جيبت عشان اعمل منه كتاب او اعمل منه كتب + +446 +00:34:12,770 --> 00:34:17,470 +تمام؟ فال .. يعني ال early مهم لتصنيع اللي هو ال + +447 +00:34:17,470 --> 00:34:21,450 +late protein synthesis نستكمل ان شاء الله للموضوع + +448 +00:34:23,030 --> 00:34:28,370 +الان الفيروسات قلنا ال RNA هو ال ultimate goal + +449 +00:34:28,370 --> 00:34:32,330 +وبالتالي هناك استراتيجية مختلفة للفيروسات للوصول + +450 +00:34:32,330 --> 00:34:37,050 +لل mRNA فمثلا أبسط وسيلة .. إذا أنا أقولك مباشرة + +451 +00:34:37,050 --> 00:34:43,830 +اعطيني الجلم، مصبوط؟ إذا بداخله منك طلعال virus من + +452 +00:34:43,830 --> 00:34:47,450 +استراتيجيته البسيطه ان هو اصلا boosted stranded + +453 +00:34:47,450 --> 00:34:51,750 +تمام و بالتالي it acts as a messenger mRNA مالوش + +454 +00:34:51,750 --> 00:34:57,670 +اي معالجه مسبقة في هذا الموضوع بمعنى it acts as a + +455 +00:34:57,670 --> 00:35:01,050 +template مباشرة لtranslationالنوع التاني اللي هو + +456 +00:35:01,050 --> 00:35:04,550 +second group بقولنا has a single strand RNA of + +457 +00:35:04,550 --> 00:35:07,110 +negative polarity طب negative polarity ماهم هي .. + +458 +00:35:07,110 --> 00:35:13,370 +هي مشرتنا انه مافيش عنا mRNA عفوا .. ريبوزوم يقرا + +459 +00:35:13,370 --> 00:35:16,690 +mRNA مباشرة لان بتاعي لازم نعمل complementation + +460 +00:35:16,690 --> 00:35:19,830 +ايش هي complementation؟ ال negative انصنع منه .. + +461 +00:35:19,830 --> 00:35:24,240 +بص في ..وبعدين خدوا بالكوا في هدا المرحلة ال + +462 +00:35:24,240 --> 00:35:28,920 +postive can act يعني لغرضين الغرض الأول انه بالفعل + +463 +00:35:28,920 --> 00:35:33,020 +mRNA وكمان ك template كقالب يصنع منه ال genome ما + +464 +00:35:33,020 --> 00:35:35,300 +هو ال genome negative stranded صح؟ فبصير + +465 +00:35:35,300 --> 00:35:39,820 +complementary له انه يصنع منه negative strand army + +466 +00:35:39,820 --> 00:35:43,880 +امثلة عليها اللي هو ال measles virus و ال rabies + +467 +00:35:43,880 --> 00:35:48,960 +virus و فيها اللي هوالهدوء اللي بيمتلكه بقطعة + +468 +00:35:48,960 --> 00:35:53,660 +واحدة على فكرة من ال RNA ال negative بينها في نوع + +469 +00:35:53,660 --> 00:35:56,940 +تاني بيحتوي على multiple pieces اللي هم ال + +470 +00:35:56,940 --> 00:36:01,260 +influenza virus خدوا بالكوا أي فيروس بيحتوي على ال + +471 +00:36:01,260 --> 00:36:04,580 +negative sense مضطر يجيب معاه انزيمات تبعده بتاعه + +472 +00:36:04,580 --> 00:36:09,580 +و non-infectious كجينوم كجينوم non-infectious the + +473 +00:36:09,580 --> 00:36:14,690 +third group has double-stranded RNADouble-stranded + +474 +00:36:14,690 --> 00:36:20,410 +يعني في عنده negative و في عنده positive لكن لأن + +475 +00:36:20,410 --> 00:36:23,410 +خلايانا لا تحتوى على أي إنزيمة تتعامل مع double + +476 +00:36:23,410 --> 00:36:27,450 +-stranded فهو يجيب برضه عشان يفصلهم و يصنع + +477 +00:36:27,450 --> 00:36:43,370 +negative و positiveالرايو فايروس الرايو + +478 +00:36:43,370 --> 00:36:43,370 +فايروس الرايو فايروس الرايو فايروس الرايو فايروس + +479 +00:36:43,370 --> 00:36:43,370 +الرايو فايروس الرايو فايروس الرايو فايروس الرايو + +480 +00:36:43,370 --> 00:36:43,550 +فايروس الرايو فايروس الرايو فايروس الرايو فايروس + +481 +00:36:43,550 --> 00:36:44,850 +الرايو فايروس الرايو فايروس الرايو فايروس الرايو + +482 +00:36:44,850 --> 00:36:46,830 +فايروس الرايو فايروس الرايو فايروس الرايو فايروس + +483 +00:36:46,830 --> 00:36:47,350 +الرايو فايروس الرايو فايروس الرايو فايروس الرايو + +484 +00:36:47,350 --> 00:36:54,930 +فايروس الرايو فايروس الرايو فايروس الرايو فايروس + +485 +00:36:54,930 --> 00:37:02,120 +الرايو فايروس الرايو فايروسالشكل الرابع من ال RNA + +486 +00:37:02,120 --> 00:37:06,500 +فيروس هم المفروض انهم تلاتة، ياما boosted، ياما + +487 +00:37:06,500 --> 00:37:09,980 +negative، ياما double-stranded فى الاسف الرابع هو + +488 +00:37:09,980 --> 00:37:13,520 +boosted strand لكنه حالة خاصة، boosted strand حالة + +489 +00:37:13,520 --> 00:37:19,080 +خاصة هو HIV، لحظة محتوى عليها قطعة و قطعة، هى + +490 +00:37:19,080 --> 00:37:24,520 +قطعتين، هدول القطعتين مش قطعتين مختلفتين، هم + +491 +00:37:24,520 --> 00:37:28,840 +identicalبغير هذا القطع التابعون الفيروس اللي + +492 +00:37:28,840 --> 00:37:31,880 +جابهم بشوية او الانفلواز التمان قطع هدلا كل قطعة + +493 +00:37:31,880 --> 00:37:36,650 +تختلف عنهذا identical الي انا سميناهم deployed + +494 +00:37:36,650 --> 00:37:41,010 +فاكرين؟ بقولنا انه كل الفيروسات hub load ماعدل + +495 +00:37:41,010 --> 00:37:47,110 +retro viruses are deployed طبعا هذا كلمة retro بقى + +496 +00:37:47,110 --> 00:37:50,370 +نهائية الخلف زمان احنا كنا نعرف ان ال flow of + +497 +00:37:50,370 --> 00:37:54,730 +information هو من ال DNA لل RNA لل protein بيسموها + +498 +00:37:54,730 --> 00:37:59,010 +ال central dogma لما اكتشفنا الفيروسات صار عندنا + +499 +00:37:59,010 --> 00:38:03,880 +يعني لأفي أشياء جديدة ساندبرج على ورا ال RNA + +500 +00:38:03,880 --> 00:38:07,400 +بالظبط هو من ال RNA ل .. و هذا مش عندنا عند + +501 +00:38:07,400 --> 00:38:10,380 +الفيرور اختار هذا الانزين تبعه و يجب ان يكون معاه + +502 +00:38:10,380 --> 00:38:16,380 +اللي هو reverse transcriptase و هذه الفكرة تبع ال + +503 +00:38:16,380 --> 00:38:19,160 +infectious nucleic acid انا شرحتها انه اذا كان + +504 +00:38:19,160 --> 00:38:24,820 +قادر على تخطي ال specificity و يعني انه عمل + +505 +00:38:24,820 --> 00:38:28,220 +infection بعد الحكي انه infectious nucleic acid + +506 +00:38:28,220 --> 00:38:29,160 +اذا ماجدرش + +507 +00:38:35,050 --> 00:38:40,030 +الشكل اللى قدامنا هذا يمكن اللى .. اللى هي المقادة + +508 +00:38:40,030 --> 00:38:43,410 +اللى بدأنا فيها لما قولنا انه كل الفيروسات + +509 +00:38:43,410 --> 00:38:46,130 +بأنواعها المختلفة double-stranded single-stranded + +510 +00:38:46,130 --> 00:38:49,030 +DNA او RNA segmented او غير segmented بتروح على + +511 +00:38:49,030 --> 00:38:55,470 +مين على انها تتحول لviral MR وانشاء الله بتوضحلكوا + +512 +00:38:55,470 --> 00:38:57,650 +الموضوع هذا لاحقا بإذن الله + +513 +00:39:02,870 --> 00:39:07,350 +once the viral mRNA of either DNA or RNA virus is + +514 +00:39:07,350 --> 00:39:11,510 +synthesized it is translated by the host ribosome + +515 +00:39:11,510 --> 00:39:14,430 +into the viral protein يبقى ياخدوا بالكم ال + +516 +00:39:14,430 --> 00:39:18,830 +ribosome متباعتنا الصحيح very fair neutral بتقول + +517 +00:39:18,830 --> 00:39:23,930 +للخليجي أنا ماليش دخل انا هاتولي اي كتاب انا بقراه + +518 +00:39:23,930 --> 00:39:29,290 +مابميز فيروس كتاب فيروس كتاب انا بعرف ان هذا ال + +519 +00:39:29,290 --> 00:39:36,840 +mRNA محتاج القياله بقراهمشاكلكوا جابلي إذا وصلتني + +520 +00:39:36,840 --> 00:39:42,920 +إذا وصلتني أنا بقرأ مالش داعو نيوترال فالموجة + +521 +00:39:42,920 --> 00:39:47,980 +بتتكوّن mRNA بوستر بولاريتي بيجي لريبوزوم و بيقرأ + +522 +00:39:47,980 --> 00:39:53,380 +طريقة القراءة الآن هو المشكلة + +523 +00:39:53,380 --> 00:39:59,720 +بيقول إنه some of which are early proteins + +524 +00:40:08,840 --> 00:40:15,380 +هذا الجدولب يوضح لنا القائمة بالفيروسات و ال + +525 +00:40:15,380 --> 00:40:21,480 +polymerase المسئولة عن تصنيع ال RNA من المادة + +526 +00:40:21,480 --> 00:40:21,960 +الوراثية + +527 +00:40:26,540 --> 00:40:31,920 +محتاجة او معتمدة على ال cell polymerases وفي DNA + +528 +00:40:31,920 --> 00:40:36,320 +viruses معتمدة على أنزيمتها نفسها فاكرين لما قلنا + +529 +00:40:36,320 --> 00:40:42,380 +ان ال box virus مابقدرش يروح على ال nucleus + +530 +00:40:42,380 --> 00:40:45,800 +وبالتالي هو محتاج ان الانزيم الخاص فيه اللي مش + +531 +00:40:45,800 --> 00:40:49,280 +هقدر يطوله من وين من النوات بتاعه ال host فهكذا + +532 +00:40:49,280 --> 00:40:55,310 +ونفس الجثة بالنسبة لل RNA virusفكرة ال precursor + +533 +00:40:55,310 --> 00:40:58,070 +polypeptide اللى شراحتها قبل قليل انه ال + +534 +00:40:58,070 --> 00:41:05,510 +translation تم ل full length mRNA و يطلع عندي + +535 +00:41:05,510 --> 00:41:11,370 +polypeptide طويل يتم تقطيعه بعد ذلك من أشهر + +536 +00:41:11,370 --> 00:41:16,770 +النماذج ان حدثنا بيكورنا فيروس و ال بيكورنا بيكو + +537 +00:41:16,770 --> 00:41:20,530 +معناه small و RNA بيكو عملي + +538 +00:41:32,060 --> 00:41:37,460 +الجينوم تبعها روية RNA acting as mRNA is + +539 +00:41:37,460 --> 00:41:41,220 +translated into a single polypeptide which is then + +540 +00:41:41,220 --> 00:41:43,780 +cleaved by a virus coded protease into various + +541 +00:41:43,780 --> 00:41:45,860 +proteins الكلام اللي حكيناه في بداية المحاضرة، + +542 +00:41:45,860 --> 00:41:48,720 +قولنا إن ال protease بيقطعها إلى functional + +543 +00:41:53,560 --> 00:41:57,960 +في البداية ففكرنا انه unique للمجموعة دي اللي + +544 +00:41:57,960 --> 00:42:01,700 +جالناه في الآخر لأن هو استراتيجية معتمدة لدى كثير + +545 +00:42:01,700 --> 00:42:06,860 +منها من البيانات التبتالي بدأنا نبحث عن فكرة انه + +546 +00:42:06,860 --> 00:42:10,060 +نستهدف من ال proteins اللي بيجدع هذا ال + +547 +00:42:10,060 --> 00:42:14,800 +polyurethane بتالي احنا بنضمن انه نخلص منه فهي + +548 +00:42:14,800 --> 00:42:18,400 +مثلا ال retro virus و ال retro virus انا هحكيلكم + +549 +00:42:18,400 --> 00:42:24,980 +بكل وضوح ان اكتشاف ال HIVعمل advancement كبيرة في + +550 +00:42:24,980 --> 00:42:28,380 +علم الفيروسات وعلم المناعة في علم الفيروسات وعلم + +551 +00:42:28,380 --> 00:42:32,920 +المناعة ال full اكتر فيروس دُرست ال life cycle + +552 +00:42:32,920 --> 00:42:36,440 +تبقى تبقى دقة هو ال HIV في محاولة من العلماء اللي + +553 +00:42:36,440 --> 00:42:42,070 +هتطبق إنهم يجدوا علاج cute لهفمن خلال دراستنا + +554 +00:42:42,070 --> 00:42:47,110 +للفيروس و لما ندرسها ال life cycle ندرسها مش عشان + +555 +00:42:47,110 --> 00:42:50,550 +احنا بدنا نعرفها و خلاص، معرفتها لا علم الا ما + +556 +00:42:50,550 --> 00:42:55,430 +نفع، لا علم الا ما نفع، البعرفة بدنا نعرف وين + +557 +00:42:55,430 --> 00:43:00,480 +استهدفها عشان نوقف اللي هو ال life cycleفلمّا + +558 +00:43:00,480 --> 00:43:04,000 +عرفنا إنه قعد أشهر الجينات تبعتها اللي هو الجاج + +559 +00:43:04,000 --> 00:43:06,580 +والبول genes اللي بيصير لهم translation برضه + +560 +00:43:06,580 --> 00:43:11,680 +بيكوّنوا بول بيبطال لازم يجسهم من ال protease إذا + +561 +00:43:11,680 --> 00:43:16,280 +أنا استغلقت ال protease أنا وقفت أهم two genes + +562 +00:43:16,280 --> 00:43:20,620 +وبالتالي وقفت عملية ال violence وreplication تبع + +563 +00:43:20,620 --> 00:43:23,460 +اللي هو ال violence وبالفعل الآن في class + +564 +00:43:27,140 --> 00:43:31,540 +فهو يستخدم في علاج الـ Retrovirus اللي هي الـ HIV + +565 +00:43:31,540 --> 00:43:36,480 +منها فى أننا كمان مثال تانى الـ Flaviviruses الـ + +566 +00:43:36,480 --> 00:43:40,960 +Flaviviruses مثال شهير عليها اللي هو الـ Hepatitis + +567 +00:43:40,960 --> 00:43:44,620 +C والـ Yellow Fever فيروس برضه بس بيعملوا + +568 +00:43:44,620 --> 00:43:47,380 +precursor polypeptide فى أننا كمان الـ Rota + +569 +00:43:50,300 --> 00:43:54,760 +النموذج التالي اللي هو ال Influenza و ال Rotavirus + +570 +00:43:54,760 --> 00:43:59,700 +عندهم segmented genome وبالتالي كل قطعة بيصيلها + +571 +00:43:59,700 --> 00:44:02,920 +transcription أو translation بتعطينا functional + +572 +00:44:02,920 --> 00:44:05,380 +إيه ال protein لأن في precursor protein و فينا + +573 +00:44:05,380 --> 00:44:11,180 +functional protein مباشرة هذه لوحة نموذج على ال + +574 +00:44:11,180 --> 00:44:14,500 +polyvirus اللي بيوضح لنا انه long polypeptide long + +575 +00:44:14,500 --> 00:44:20,000 +mRNA بتكون منه long أو full lengthوهذا طبعا ال + +576 +00:44:20,000 --> 00:44:27,620 +cleavage عبارة عن تحويل من inactive إلى active، من + +577 +00:44:27,620 --> 00:44:30,120 +non-functional إلى functional، ونفس الشيء اللي + +578 +00:44:30,120 --> 00:44:32,940 +بتفكره عندي هنا كمان لل retrovirus + +579 +00:44:38,810 --> 00:44:43,910 +طبعا ال virusات اللي عندها proteases و اللي همية + +580 +00:44:43,910 --> 00:44:46,470 +طبية فيه نماذج عليها في ال dead well هذا ب + +581 +00:44:46,470 --> 00:44:50,250 +coronavirus, bloody virus, toga virus, coronavirus + +582 +00:44:50,250 --> 00:44:54,470 +و ال retroviruses طبعا كل واحد منهم بيوضحلنا في ال + +583 +00:44:54,470 --> 00:44:58,610 +dead well إيش هو ال polypeptide أو ال polyprotein + +584 +00:44:58,610 --> 00:45:02,890 +و وين بيتم عملية اللي هو site of cleavage تخيلوا + +585 +00:45:02,890 --> 00:45:05,230 +ال retroviruses ال cleavage وين صار؟ + +586 +00:45:08,130 --> 00:45:12,950 +في ال bonding layer يعني و الجريب ما تطلع الفيروس + +587 +00:45:12,950 --> 00:45:18,330 +بيصير فيه عملية cleavage لذلك إذا استهدفنا بنمنع + +588 +00:45:18,330 --> 00:45:20,670 +ال bonding تبعه إذا منعنا ال bonding تبعه مايرحق + +589 +00:45:20,670 --> 00:45:24,750 +إنه بطل يطلع إلى الخلايا الأخرى و لن يكون بعمل + +590 +00:45:24,750 --> 00:45:29,710 +infection ل other cells الفكرة + +591 +00:45:29,710 --> 00:45:33,790 +هذه ماكنتش أحكي فيها كثير لكن هي الفكرة إنه دائما + +592 +00:45:33,790 --> 00:45:39,650 +أبدا في المادة الوطنيةالـ T بدو يجيبلها A والـ G + +593 +00:45:39,650 --> 00:45:43,490 +بدو يجيبلها C أو الـ C يجيبلها وها كده فإذا حاطوا + +594 +00:45:43,490 --> 00:45:48,530 +انتوا في كل الفيروسات اللي شوفناها إذا المادة اللي + +595 +00:45:48,530 --> 00:45:54,170 +بيبقى بيها double stranded بدها تنقسم تنقسم إلى + +596 +00:45:54,170 --> 00:45:58,770 +two strands تبعها ويتكملوا complementary صح؟ خلصنا + +597 +00:45:58,770 --> 00:46:02,470 +منهم إذا كان single strand بدو يتكمل إذا كان + +598 +00:46:02,470 --> 00:46:04,670 +negative بدو positive وإذا كان positive بدو + +599 +00:46:04,670 --> 00:46:09,910 +negative وها كدهفكل الفكرة معتمدة على ال + +600 +00:46:09,910 --> 00:46:13,910 +complementarity اللي هي التكامل انه فيروس او مادة + +601 +00:46:13,910 --> 00:46:19,350 +وضعفية كامل ومادة وضعفية اخر واذا ربحوا في الدول + +602 +00:46:19,350 --> 00:46:23,370 +الغالية كتير ال parental genome و ال progeny + +603 +00:46:23,370 --> 00:46:27,430 +genome اذا حد بقدر يجيلي اختلاف بكون شاطر + +604 +00:46:31,670 --> 00:46:37,210 +مش اختلاف، يبقى مش كلكم شطرين، لأنه أصلا الهدف أنه + +605 +00:46:37,210 --> 00:46:41,930 +أنا أطلّي النسخ برجيني نفس ال .. الventrella، صح + +606 +00:46:41,930 --> 00:46:45,570 +دلوقتي؟ طب أنا أجي postive sense، single strand + +607 +00:46:45,570 --> 00:46:48,950 +عرنية، و نفس الشي اللي برجيني هيكون، لكن ال + +608 +00:46:48,950 --> 00:46:52,870 +intermediate عكسه، ال intermediate اللي هو داخل ال + +609 +00:46:52,870 --> 00:46:55,510 +.. و أنا بعمل replication، مش قولنا postive sense + +610 +00:46:55,510 --> 00:46:57,850 +اللي بتكون له ايه؟ negative sense، هاي ال + +611 +00:46:57,850 --> 00:46:59,390 +complementary اللي احنا بنحكيه + +612 +00:47:02,710 --> 00:47:06,030 +Assembly لان بني ليزا بلقت فيها انت بتذكرها و + +613 +00:47:06,030 --> 00:47:09,790 +بتلكوا ان ال assembly معتمد بالاساس على ال + +614 +00:47:09,790 --> 00:47:13,250 +interaction اللي احنا مش فاهمينه ما بين ال protein + +615 +00:47:13,250 --> 00:47:18,150 +و المادة الوراثية وهذا اعتقادنا انه ناتج عن ال + +616 +00:47:18,150 --> 00:47:20,490 +chemical structure أو ال chemical composition + +617 +00:47:20,490 --> 00:47:26,410 +للمادة تانية المادة DNA او ال RNA وهذا الكلام + +618 +00:47:26,410 --> 00:47:27,930 +للأسف يعني .. + +619 +00:47:30,950 --> 00:47:33,330 +النقطة اللى حكيتها لكوا يعنى انه لما حبطناهم مع + +620 +00:47:33,330 --> 00:47:41,050 +بعض اتجمعوا اعملوا virion كامل اللقطة + +621 +00:47:41,050 --> 00:47:43,010 +اللى امامكوا هي اللقطة اللى شوفناها في ال + +622 +00:47:43,010 --> 00:47:50,090 +animation انه فيروس هو طالع من الخلية بياخد جزء من + +623 +00:47:50,090 --> 00:47:54,910 +ال cell membrane بعد ما يكون قد يعني وضع فيه اللي + +624 +00:47:54,910 --> 00:47:56,470 +هو ال glycoproteins + +625 +00:48:01,480 --> 00:48:05,680 +receptor لل next infection مهانة ميزة تقولنا ال + +626 +00:48:05,680 --> 00:48:08,680 +envelope viruses إنها بتسهل عليها عملية اللي هو + +627 +00:48:08,680 --> 00:48:12,140 +الإرتباط أو الإصابة هذا الموضوع مش هاجده أكمله + +628 +00:48:12,140 --> 00:48:16,240 +اليوم يعني في ضيق الوقت فهو ايه يعني ممكن تلت أربع + +629 +00:48:16,240 --> 00:48:20,080 +شرائع أرجو أن تقراهم لحالك و اللي عنده أي استفسار + +630 +00:48:20,080 --> 00:48:24,980 +ان شاء الله أنا باستقبله بس بدي أختم بيعني بكلمات + +631 +00:48:24,980 --> 00:48:25,640 +بسيطة + +632 +00:48:31,750 --> 00:48:35,310 +الطلبة يعني تبين انه مابنجرش نحكم عليها من خلال + +633 +00:48:35,310 --> 00:48:37,910 +الامتحان يعني في ناس عامة ال presentation و الصحي + +634 +00:48:37,910 --> 00:48:41,550 +كانت ال presentation تبعها جيجدة جدا هذا بيقودنا + +635 +00:48:41,550 --> 00:48:44,310 +إلى انه يعني انا شخصيا انا عندي قنعة من زمان انه + +636 +00:48:44,310 --> 00:48:48,450 +موضوع تقييم الطلبة من خلال الامتحان هذا يعني وسيلة + +637 +00:48:48,450 --> 00:48:52,530 +من الوسائل ولكن ليس هو التقييم الحقيقي واللي حتى + +638 +00:48:52,530 --> 00:48:56,490 +بيرسب عندي بضل الاحترام والتقدير تبعه موجود يعني + +639 +00:48:56,490 --> 00:49:01,380 +بمعنى اخر مش لأنه هو رسبأو بما يكون ظرف الاخر لكن + +640 +00:49:01,380 --> 00:49:05,200 +احنا بنقولكوا ان هذا مساق ليس للنسيان ليس للامتحان + +641 +00:49:05,200 --> 00:49:09,440 +وبعدين نتكه من أدمغاتنا ونمشي هذا مساق احنا + +642 +00:49:09,440 --> 00:49:13,360 +محتاجينه طول الطريق وبعد الطريق وبعد اللي هو + +643 +00:49:13,360 --> 00:49:17,620 +التخرج وبالتالي انا بأمن منكم انكوا تدرسوه بعناية + +644 +00:49:17,620 --> 00:49:22,340 +اكثر من انه ندرس عشان بدنا نجيب علامات في الختام + +645 +00:49:22,340 --> 00:49:26,440 +ندعو الله ان يوفقكم إلى ما فيه الخير والسلام عليكم + +646 +00:49:26,440 --> 00:49:27,080 +ورحمة الله وبركاته + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..0b8a1438954f311314070b522b0a97ff6424a8b1 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc_postprocess.srt @@ -0,0 +1,2580 @@ +1 +00:00:20,920 --> 00:00:23,120 +السلام عليكم ورحمة الله وبركاته بسم الله الرحمن + +2 +00:00:23,120 --> 00:00:27,120 +الرحيم أنواع المحاضرة اليوم هو ال sterilization + +3 +00:00:27,120 --> 00:00:31,760 +and disinfection وهذا الموضوع يأتي بعد سلسلة + +4 +00:00:31,760 --> 00:00:36,560 +مواضيع كلها كانت مركزة على تركيب الخلية ومن ثم + +5 +00:00:36,560 --> 00:00:40,820 +كيفية تنمية الخلية وما هي العوامل اللي تؤثر على + +6 +00:00:40,820 --> 00:00:45,420 +النمو بدل منمي البكتيريا الحقيقة أنا ذكرت في نهاية + +7 +00:00:45,420 --> 00:00:49,560 +المحاضرة السابقةإنه ليس من الصعب تنمية البكتيريا + +8 +00:00:49,560 --> 00:00:54,020 +وإنما اللي صعب أو أصعب هو كيف أنا أتخلص من + +9 +00:00:54,020 --> 00:01:00,580 +البكتيريا واليوم هنبدأ الموضوع هذا ببعض المصطلحات + +10 +00:01:00,580 --> 00:01:05,460 +ومن ثم بعض الأدوات وبعض التقنيات المستخدمة في + +11 +00:01:05,460 --> 00:01:07,960 +السيطرة على ال micro organism أو ال control of + +12 +00:01:07,960 --> 00:01:13,950 +microorganismبنعرف إنه من خلال التعريف اللي مورده + +13 +00:01:13,950 --> 00:01:17,350 +لنا sterilization إنه مصطلح مطلق كلمة مصطلح مطلق + +14 +00:01:17,350 --> 00:01:22,210 +يعني لا يقبل الاستثناء بمعنى إنه بقولنا هنا is the + +15 +00:01:22,210 --> 00:01:26,810 +killing or removal of all microorganismsكلمة all + +16 +00:01:26,810 --> 00:01:31,170 +تعني المتبقاش zero وبالتالي هو بالفعل absolute + +17 +00:01:31,170 --> 00:01:35,510 +term بدنا دايما نميزه يعني مداش نخلط فيهم الأيام و + +18 +00:01:35,510 --> 00:01:40,310 +نقول sterilization و disinfection و disinfection + +19 +00:01:40,310 --> 00:01:44,550 +هم نفس المصطلح لأ باختلفوا عن بعض اختلاف كبير ال + +20 +00:01:44,550 --> 00:01:49,330 +sterilization absolute term بينما disinfection لأ + +21 +00:01:49,330 --> 00:01:50,730 +بيقبل ال compromise + +22 +00:01:54,260 --> 00:01:56,960 +إذا بتلاحظوا ال sterilization is usually carried + +23 +00:01:56,960 --> 00:02:01,980 +out using autoclaving ال autoclaving أو ال + +24 +00:02:01,980 --> 00:02:05,400 +autoclave هو أدا من الأدوات اللي كلكم بتعرفوها في + +25 +00:02:05,400 --> 00:02:09,720 +المنزل اللي هي عبارة عن pressure cooker أو تنجرة + +26 +00:02:09,720 --> 00:02:15,240 +الضغط احنا بنعرف أنه نقطة غليان الماء هي 100 درجة + +27 +00:02:15,240 --> 00:02:19,360 +100 أوي 100 درجة 100 أوي لو سخننا نص ساعة ساعة + +28 +00:02:19,360 --> 00:02:25,290 +ساعتين تلاتة في الآخر بتضلها 100100 درجة مئوية إذا + +29 +00:02:25,290 --> 00:02:30,670 +كيف نحصل على درجة أعلى من تلك الدرجة؟ عن طريق ضغط + +30 +00:02:30,670 --> 00:02:37,810 +بخار الماء نضغط البخار فترتفع الحرارة بمقدار الضغط + +31 +00:02:37,810 --> 00:02:42,970 +يعني بمثال مولود اليوم على السطولة لو كان الضغط 15 + +32 +00:02:42,970 --> 00:02:47,650 +PSI 15 PSI هو المصطلح الأكثر شروعا في الاستخدام + +33 +00:02:47,650 --> 00:02:52,440 +وهو عبارة عن pound per square inchsquare inch اللي + +34 +00:02:52,440 --> 00:02:55,980 +هي بوصة مربعة وال bound هو راطلينجيز يعني هي من + +35 +00:02:55,980 --> 00:03:01,180 +قياسة إذا كانت ضغط 15 psi بتكون درجة حرارة الماء + +36 +00:03:01,180 --> 00:03:08,440 +121 درجة مائوية لو احتفظنا بهذه الدرجة و هذا الضغط + +37 +00:03:08,440 --> 00:03:17,940 +لمدة 15 دقيقة تمام هنا نحصل على تعقيم بمعنى أن كل + +38 +00:03:17,940 --> 00:03:24,330 +المخلوقاتالطبية اللى بنعرفها تموت عند هذه الدرجة + +39 +00:03:24,330 --> 00:03:29,910 +لكن بدنا نكون متأكدين أنه بالفعل الدرجة اللى + +40 +00:03:29,910 --> 00:03:32,110 +قدامنا اللى على ال .. اللى .. اللى على ال counter + +41 +00:03:32,110 --> 00:03:36,790 +إنها 121 وإن الضغط صحيح وإن ال timing بدأ .. متى + +42 +00:03:36,790 --> 00:03:41,570 +بدأ ال timing؟ بعد ما وصلت الحرارة ل 121 درجة + +43 +00:03:41,570 --> 00:03:45,790 +مئوية يعني مش من أول العملية لأ بعد وصول العملية + +44 +00:03:45,790 --> 00:03:50,940 +إلى 121 درجة مئوية ثلاث ألقاب سهلينمية واحد عشرين + +45 +00:03:50,940 --> 00:03:52,820 +خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر + +46 +00:03:52,820 --> 00:03:55,560 +خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر + +47 +00:03:55,560 --> 00:03:58,980 +خمستاشر + +48 +00:03:58,980 --> 00:04:02,060 +خمستاشر + +49 +00:04:02,060 --> 00:04:04,100 +خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر + +50 +00:04:04,100 --> 00:04:04,400 +خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر + +51 +00:04:04,400 --> 00:04:04,740 +خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر + +52 +00:04:04,740 --> 00:04:05,620 +خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر + +53 +00:04:05,620 --> 00:04:17,900 +خمستاشر خمستاشر خمستاشر خمستاشر خمستاشر + +54 +00:04:17,900 --> 00:04:23,410 +خمالشاشة الطبية على سبيل المثال لكن هناك أدوات قد + +55 +00:04:23,410 --> 00:04:29,370 +تتدمر بالتعقيم بمعنى بروتين لو بنعقم ال albumin + +56 +00:04:29,370 --> 00:04:32,770 +تبع البيض وحطيناها في ال autoclave درجة الحرارة + +57 +00:04:32,770 --> 00:04:36,930 +هتكون إيه؟ عالي جدا بحيث إن ال albumin هيصير له + +58 +00:04:36,930 --> 00:04:41,090 +coagulation بيبطل السائل بيصير صلب طب في هذه + +59 +00:04:41,090 --> 00:04:44,010 +الحالة أنا بدّي البروتين هذا بدّي ال albumin + +60 +00:04:44,010 --> 00:04:47,170 +بدّيها سائل ليش هي صلب؟ يبقى ماقدرش أعقمه بال + +61 +00:04:47,170 --> 00:04:50,410 +autoclaveيبقى الـ autoclave على الرغم من شيوعه + +62 +00:04:50,410 --> 00:04:55,430 +وانتشاره وكثرة استخداماته اللي انه غير صالح لكل + +63 +00:04:55,430 --> 00:05:00,250 +الأغراض اللي احنا من أجلها بنعقم الأشياء فبقولنا + +64 +00:05:00,250 --> 00:05:04,050 +مثلا surgical instruments that may rust رست يعني + +65 +00:05:04,050 --> 00:05:09,430 +بتصضي في وجود اللي هو البخار المال، لا يستطيعون + +66 +00:05:09,430 --> 00:05:13,630 +يعقينه ال autoclave جهاز لابتوب زي هذا لو صار عليه + +67 +00:05:13,630 --> 00:05:17,330 +spill، bacterial spill وانكبت عليه مزرعة بكتيريا، + +68 +00:05:17,330 --> 00:05:21,810 +وبدأ نعقمهبنفع نحطه في الوتو كليف؟ طيب بنفع نضيل + +69 +00:05:21,810 --> 00:05:26,390 +عليه كلور؟ مانفعش يبجي بأحسن وسيلة تعقيمه في هذه + +70 +00:05:26,390 --> 00:05:31,350 +الحالة للغاز إنه نجيب مادة غازية قاتلة الغاز + +71 +00:05:31,350 --> 00:05:36,850 +بيخترق الأشياء دون أن يحدث بلل أو wet وبالتالي + +72 +00:05:36,850 --> 00:05:41,070 +بتظل الدوائل الكهربائية شغالة إذن فين دي أشكال + +73 +00:05:41,070 --> 00:05:44,730 +وأنواع متختلفة من اللي هو المواد المستخدمة له ال + +74 +00:05:44,730 --> 00:05:48,530 +sterilization ألا بالله حاجة ذكرت واحد تعريفرقم + +75 +00:05:48,530 --> 00:05:53,550 +اتنين ذكرت القالية اللى بتتم من خلالها the math + +76 +00:05:53,550 --> 00:05:57,750 +اللى زى اللى هو ال autoclaving ثم اتحدثت على بداء + +77 +00:05:57,750 --> 00:06:02,890 +الأخرى اتحدثت عن مثلا ال ethane oxide اللى هو + +78 +00:06:02,890 --> 00:06:06,930 +الجاز فى هذه الحالة وفى كمان حالة تالتة موجودة على + +79 +00:06:06,930 --> 00:06:10,610 +اللابحة اللى هى ال filtrationأنا أقول كده بشري ال + +80 +00:06:10,610 --> 00:06:14,070 +البيومين صح؟ ال البيومين إذا أنا بديها protein + +81 +00:06:14,070 --> 00:06:17,870 +السائل كما هو بمرره عبر filter ال filter هذا + +82 +00:06:17,870 --> 00:06:21,310 +مساماته اللي هتسبح بمرور البكتيريا و الفيروسات + +83 +00:06:21,310 --> 00:06:24,990 +وبالتالي كل ما يمر أو لما السائل يمر عبره + +84 +00:06:24,990 --> 00:06:30,050 +البكتيريا و الفيروسات تحجز إلى الأعلى و بنزل + +85 +00:06:30,050 --> 00:06:33,910 +نستقبل السائل في انبوبة معقمة وبالتالي سيجعلنا + +86 +00:06:33,910 --> 00:06:38,380 +تعقيم بال filtration إذن هناك وسائل مختلفةبس + +87 +00:06:38,380 --> 00:06:40,960 +بيتلاحظوا شغل من اللوحة هذه قبل ما ننتقل للوحة + +88 +00:06:40,960 --> 00:06:44,920 +تانية ان هناك من الوسائل اللي ذكرناها وسائل + +89 +00:06:44,920 --> 00:06:49,000 +ميكانيكية زي ال filtration وسائل فيزيائية زي + +90 +00:06:49,000 --> 00:06:54,240 +الحرارة وسائل كيميائية زي اللي هو الغاز إذا غالبا + +91 +00:06:54,240 --> 00:06:58,220 +هنقسم ال methods إلى physical and chemical methods + +92 +00:06:58,220 --> 00:07:02,240 +لكن لسه ما خلصنا من التعريفات وبالتالي بدنا نكمل + +93 +00:07:02,240 --> 00:07:06,870 +موضوع التعريفاتأنا من البداية قاعد بحذر من الخلط + +94 +00:07:06,870 --> 00:07:11,350 +ما بين المصطلحين اللي هو sterilization اللي هو ال + +95 +00:07:11,350 --> 00:07:12,490 +sterilization اللي هو ال sterilization اللي هو ال + +96 +00:07:12,490 --> 00:07:13,710 +sterilization اللي هو ال sterilization اللي هو ال + +97 +00:07:13,710 --> 00:07:14,690 +sterilization اللي هو ال sterilization اللي هو ال + +98 +00:07:14,690 --> 00:07:15,710 +sterilization اللي هو ال sterilization اللي هو ال + +99 +00:07:15,710 --> 00:07:16,130 +sterilization اللي هو ال sterilization اللي هو ال + +100 +00:07:16,130 --> 00:07:16,550 +sterilization اللي هو ال sterilization اللي هو ال + +101 +00:07:16,550 --> 00:07:16,950 +sterilization اللي هو ال sterilization اللي هو ال + +102 +00:07:16,950 --> 00:07:17,450 +sterilization اللي هو ال sterilization اللي هو ال + +103 +00:07:17,450 --> 00:07:17,990 +sterilization اللي هو ال sterilization اللي هو ال + +104 +00:07:17,990 --> 00:07:18,150 +sterilization اللي هو ال sterilization اللي هو ال + +105 +00:07:18,150 --> 00:07:18,770 +sterilization اللي هو ال sterilization اللي هو ال + +106 +00:07:18,770 --> 00:07:22,290 +sterilization اللي هو ال sterilization اللي هو ال + +107 +00:07:22,290 --> 00:07:25,210 +sterilization اللي هو ال sterilization اللي هو ال + +108 +00:07:25,210 --> 00:07:29,310 +sterilization اللي هو ال sterilization اللي + +109 +00:07:36,910 --> 00:07:40,610 +الان في مصطلح او مصطلحين بدنا ننتبه لهم جيدا + +110 +00:07:40,610 --> 00:07:47,190 +disinfectants ومصطلح تاني اسمه antiseptic انت + +111 +00:07:47,190 --> 00:07:53,570 +هتكلم عن مين؟ disinfection ال antiseptic او ال + +112 +00:07:53,570 --> 00:08:00,390 +disinfectant بيحمل نفس التعريف مادة تستخدم لمين؟ + +113 +00:08:02,000 --> 00:08:06,960 +لكن لماذا ميزنا بينهم بالاسم ال disinfectants + +114 +00:08:06,960 --> 00:08:11,360 +تستخدم لل inanimate objects يعني الأسطح الغير حية + +115 +00:08:11,360 --> 00:08:16,120 +بينما ال antiseptic بنستخدمه للأسطح الحية و لنفترض + +116 +00:08:16,120 --> 00:08:20,600 +الجل يعني الكحول اللي بنستخدمه اللي هو ال 70% + +117 +00:08:20,600 --> 00:08:25,200 +alcohol اللي بنستخدمه ل skin disinfection هذا + +118 +00:08:25,200 --> 00:08:29,630 +عبارة عن عياش antisepticلو استخدمته لمسح الطاولة + +119 +00:08:29,630 --> 00:08:32,830 +أو تطير الطاولة بيكون اسمها يبقى هو اللي انا + +120 +00:08:32,830 --> 00:08:37,870 +استخدمناها لغرضين مختلفين فأخد اسمين مختلفين أخد + +121 +00:08:37,870 --> 00:08:40,510 +مرة Antiseptic لما استخدمته على الجلد و أخد اسم + +122 +00:08:40,510 --> 00:08:46,290 +تاني اعتقده من الصورة و أضحى في هذا المجال طيب ما + +123 +00:08:46,290 --> 00:08:49,970 +الذي يجعلنا نقرر استخدام مادة على الجلد و مادة + +124 +00:08:49,970 --> 00:08:54,070 +أخرى على الأسطح عشان تقول انت ما تأثرش على اللي + +125 +00:08:54,070 --> 00:08:58,290 +أنا بدأت .. يعني بدأ أطهرهيعني لو .. لو افترضنا + +126 +00:08:58,290 --> 00:09:00,950 +انه مية النار .. مية النار اللي هي الhydrochloric + +127 +00:09:00,950 --> 00:09:05,130 +acid قاطلة للبكتيريا، اتميزاش نحطها على الجلد؟ + +128 +00:09:05,130 --> 00:09:07,070 +احنا طبعا .. احنا مستخدمهااش، لكن بستخدم + +129 +00:09:07,070 --> 00:09:10,710 +الchlorine على سمك متر، الchlorine معروف عنه قاطل + +130 +00:09:10,710 --> 00:09:15,870 +جيد للبكتيريا، لكن بيعمل skin irritation، يعني + +131 +00:09:15,870 --> 00:09:19,350 +تهيش فيه الجلد، وقد يعمل skin burn، كمان حرق في + +132 +00:09:19,350 --> 00:09:23,170 +الجلد، طب نستخدموش على الجلد، بستخدموه على .. على + +133 +00:09:23,170 --> 00:09:27,800 +أسطحالأيودين أقل حد من مين من الكلورين، مع أنه + +134 +00:09:27,800 --> 00:09:31,340 +التنوع من نفس الفصيلة الكلورين .. أقل الهالوجينس، + +135 +00:09:31,340 --> 00:09:36,580 +لكن نستخدمه على مين؟ على الجلب، ما نستخدموه على + +136 +00:09:36,580 --> 00:09:42,680 +الأسطح لأنه بيترك stain، أثر، مظبوط؟ فهو mild على + +137 +00:09:42,680 --> 00:09:45,980 +ال skin و سيء لمين؟ لأ، الأسطح، إذا اللي بيقالينا + +138 +00:09:45,980 --> 00:09:49,580 +نقرر إنه نستخدمه لسه infected أو unseptic هو ال + +139 +00:09:49,580 --> 00:09:52,660 +property استبعته، هل هو toxic، هل بيعمل stain، + +140 +00:09:52,660 --> 00:09:56,550 +أعطيكم مثال تالتبتعرفوا ال crystal violet ال + +141 +00:09:56,550 --> 00:10:00,610 +crystal violet اللي هو اسم تاني جينشان فيولت هذا + +142 +00:10:00,610 --> 00:10:04,370 +معروف عنه المركب انه عنده قدرة على قتل البكتيريا + +143 +00:10:04,370 --> 00:10:08,610 +واستخدمناه في وسط اسم الماكونكي بتعرف الماكونكي؟ + +144 +00:10:08,610 --> 00:10:14,230 +الماكونكي selective media أضفنا عليه crystal + +145 +00:10:14,230 --> 00:10:18,250 +violet حتى نمنع ال gram-bostig بكتيريا يبقى هو + +146 +00:10:18,250 --> 00:10:22,630 +القاتل لمين؟ لل gram-bostigطيب ليش ما نستخدمه احنا + +147 +00:10:22,630 --> 00:10:26,450 +في ال disinfection و ال antiseptic؟ و للأسف + +148 +00:10:26,450 --> 00:10:30,270 +ماستخدموهش للغرضين لأنه بيترك stain لو انا + +149 +00:10:30,270 --> 00:10:33,950 +استخدمته على أيدي بدي أسبوع أو أسبوعين حتى اللون + +150 +00:10:33,950 --> 00:10:38,970 +يروح بعد غسيل مستمر بيجيب و ترك أثر وبالتالي لم + +151 +00:10:38,970 --> 00:10:42,670 +نستخدمه لا على الأسطح ولا على .. استخدمنا ليه؟ + +152 +00:10:42,670 --> 00:10:45,530 +أغراض معينة زي مثلا كنا في الأوساط الزراعية + +153 +00:10:49,180 --> 00:10:51,320 +هذه اللغة مهمة كثير كثير كثير كثير كثير كثير كثير + +154 +00:10:51,320 --> 00:10:51,680 +كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير + +155 +00:10:51,680 --> 00:10:56,700 +كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير + +156 +00:10:56,700 --> 00:11:04,340 +كثير كثير كثير + +157 +00:11:04,340 --> 00:11:05,640 +كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير + +158 +00:11:05,640 --> 00:11:07,980 +كثير كثير كثير كثير كثير كثير كثير كثير كثير كثير + +159 +00:11:07,980 --> 00:11:17,000 +كثير كثير كثير كثير كثير + +160 +00:11:17,000 --> 00:11:25,230 +كثيردرجات لغاية ما الان بدنا نطلع على نقطتين + +161 +00:11:25,230 --> 00:11:28,430 +مهمتين او مهمين في عملية ال killing لل micro + +162 +00:11:28,430 --> 00:11:34,910 +organism انه لما انا بدي افحص قدرة agent على قتل + +163 +00:11:34,910 --> 00:11:38,650 +البكتيريا بعدش البكتيريا اللي ماتت بعد البكتيريا + +164 +00:11:38,650 --> 00:11:42,810 +اللي بقيت حية هذا اللي بقدر اعمله انا انا بقدر اعد + +165 +00:11:42,810 --> 00:11:46,570 +البكتيريا الحية اما اللي ماتت مابقدرش اعدها فبكون + +166 +00:11:46,570 --> 00:11:51,630 +عارف العادل اصليولمّا البكتيريا الحيّة قعدّها، + +167 +00:11:51,630 --> 00:11:55,250 +بتروح وضّل منها في الميت، فإحنا غالبًا بنتعامل مع + +168 +00:11:55,250 --> 00:12:00,690 +البكتيريا التي بقيت حيّة، ال survivors، من + +169 +00:12:00,690 --> 00:12:05,590 +البكتيريا، بقولنا إن ال number of survivors ده + +170 +00:12:05,590 --> 00:12:10,810 +البكتيريا تتناسب تناسبا عكسيا مع C و T، إيش ال C و + +171 +00:12:10,810 --> 00:12:14,990 +T؟ ال C هو ال concentration of the killing agent، + +172 +00:12:14,990 --> 00:12:19,810 +أعتقد أنه بقولكوا قرأته على اللوحةأو على البطل تبع + +173 +00:12:19,810 --> 00:12:24,930 +الكحول اللي في المختبر 70% الكحول و كلمة 70% هي + +174 +00:12:24,930 --> 00:12:29,930 +concentration ليش ما بيقولنا 50% 80% مظبوط يبقى في + +175 +00:12:29,930 --> 00:12:35,230 +الآخر 70% معناها concentration the length of time + +176 +00:12:35,230 --> 00:12:40,610 +agent is applied انتوا بتعرفوا انه الزمن عامل مهم + +177 +00:12:40,610 --> 00:12:45,310 +في كل حاجة و أعطيكوا مثال بسيط لو ولعنا و لع أو + +178 +00:12:45,310 --> 00:12:50,690 +بلعك و لع الغازحطينا إيدينا على مسافة مثلا عشرين + +179 +00:12:50,690 --> 00:12:58,430 +صنطي هل بنشعر بلسحة النار فورا ولا بعد فترة معينة + +180 +00:12:58,430 --> 00:13:02,170 +نقدرش نتحمل الحرارة فالتأدير ايش كان عبارة عن + +181 +00:13:02,170 --> 00:13:06,810 +تراكمي مع الزمن تراكمي مع الزمن يبقى ال exposure + +182 +00:13:06,810 --> 00:13:12,010 +time أوالـ length of time ailent is applied is + +183 +00:13:12,010 --> 00:13:16,090 +very essential مهم جدا يبقى فيه two factors ايش هم + +184 +00:13:16,090 --> 00:13:20,590 +كم مرة؟ ال concentration و ال time بقولنا ان ال + +185 +00:13:20,590 --> 00:13:23,710 +number of survivors تلاتة و تلاتة سببين عكسيا مع + +186 +00:13:23,710 --> 00:13:27,630 +ال concentration و ال time يعني كلما زاد ال time + +187 +00:13:27,630 --> 00:13:31,630 +كلما زاد ال concentration كلما قل عدد اسم .. مش كل + +188 +00:13:31,630 --> 00:13:36,940 +.. يعني كلما تكون علاقة عكسية قلعكسيا ما بيحكي انه + +189 +00:13:36,940 --> 00:13:40,560 +كل ما زاد الزمن كل ما زاد ال concentration كل ما + +190 +00:13:40,560 --> 00:13:42,920 +قل على ال survival، يعني بقى ماناخة كل ما زاد + +191 +00:13:42,920 --> 00:13:46,720 +القتل، كل ما زاد القتل، الجالسة النقطة كتير معلومة + +192 +00:13:46,720 --> 00:13:51,780 +أو مفهومة وبيجب أن نستفيد منها، بنفعك نيجي نمسح + +193 +00:13:51,780 --> 00:13:55,400 +الأسطح اللي في المستشفيات عشان بس نقول والله + +194 +00:13:55,400 --> 00:14:00,100 +طهرنا، لازم نترك وقت زمنمعتمد على ال + +195 +00:14:00,100 --> 00:14:02,640 +concentration، يعني لو كانت ال concentration + +196 +00:14:02,640 --> 00:14:06,420 +خمسين، حسيبه زمن أطول من لو كانت ال concentration + +197 +00:14:06,420 --> 00:14:10,000 +سبعين، هدول العاملين متفاعلين مع بعض، ال + +198 +00:14:10,000 --> 00:14:13,480 +concentration هي الزمن طب خدوا بالك، احنا بعض + +199 +00:14:13,480 --> 00:14:17,460 +الأحيان هدول العاملين مع بعض، يعني بيجبرونا أن + +200 +00:14:17,460 --> 00:14:22,740 +نفكر بطرق متنوعة الكحول على سبيل المثال، تركيز + +201 +00:14:22,740 --> 00:14:26,390 +خمسين في المية بيقتل البكتيريالكن الزمن اللازم بده + +202 +00:14:26,390 --> 00:14:30,070 +تلت دقائق تقريبا، تلت دقائق، في حد فينا بيغسل إيدي + +203 +00:14:30,070 --> 00:14:34,270 +بالكحول، بيقعد فيهم تلت دقائق، كام دقيق، كام ثاني، + +204 +00:14:34,270 --> 00:14:37,430 +ثواني بنستخدم صح ولا ثواني، وانتهى الموضوع، ف + +205 +00:14:37,430 --> 00:14:40,450 +practically احنا بنرفع ال concentration عشان نقل + +206 +00:14:40,450 --> 00:14:44,210 +من الزمن في هذه الحالة، لكن خدوا بالك، ولي كل + +207 +00:14:44,210 --> 00:14:48,750 +قاعدة دايما فيها شوية، الكحول بنسبة مائة بالمائة + +208 +00:14:54,050 --> 00:14:59,230 +الجهول بنسبة مائة بالمائة غير فعال، بس بنسبة تسعين + +209 +00:14:59,230 --> 00:15:03,190 +بالمائة فعال، وتمانين بالمائة فعال، وسبعين، وستين، + +210 +00:15:03,190 --> 00:15:08,250 +وخمسين، ليش اخترنا سبعين بالذات؟لجينا إن الـ 70 at + +211 +00:15:08,250 --> 00:15:13,070 +one second أو two seconds كافر القتل طب و أنا بقدر + +212 +00:15:13,070 --> 00:15:16,170 +أمسح إيدي بأقل من one second أصلا بقدرش وبالتالي + +213 +00:15:16,170 --> 00:15:19,730 +مناسبة طب مش مختلف بستخدام اش تمانين تمانين أغلى + +214 +00:15:19,730 --> 00:15:24,790 +تمانين بتكلفني فلوس concentration أعلى مظبوط + +215 +00:15:24,790 --> 00:15:28,710 +بتكلفني مادة فعالة أعلى وبالتالي فيها فلوس أكتر + +216 +00:15:28,710 --> 00:15:31,970 +يبقى في أكتر من عامل بيخلنا نختار إيه ال rise + +217 +00:15:31,970 --> 00:15:35,920 +concentrationلو روحنا على المستشفى و قلنا لهم يا + +218 +00:15:35,920 --> 00:15:39,060 +جماعة إيش في عندكم مواد مطهرة بتستخدموها في + +219 +00:15:39,060 --> 00:15:44,800 +المستشفى؟ هيذكروننا مجموعة أسماء، هنسألهم ألا يوجد + +220 +00:15:44,800 --> 00:15:50,260 +لديكم نوع واحد بيغني عن كل هدول الأشياء، هيقولوننا + +221 +00:15:50,260 --> 00:15:55,280 +لأسف لأ، للأسف لأ، ذكرنا أحد الأسباب أنه ممكن يكون + +222 +00:15:55,280 --> 00:15:59,760 +.. أعطيكم مثال أنا من حياتي كل يومية، بدك تشتري + +223 +00:15:59,760 --> 00:16:05,010 +laptop، هبتقولي بدي مواصفاتبدي كارت شاشة مواصفاته + +224 +00:16:05,010 --> 00:16:08,510 +مش عارف اتنين جيجا، بدي hard disk واحد تيرة، بدي + +225 +00:16:08,510 --> 00:16:12,630 +ال memory تبعته مش عارف ال cash تبعه وكده، انهم + +226 +00:16:12,630 --> 00:16:15,790 +بروح السوق بنلاقي تلاتة من الاربع مواصفات اللي + +227 +00:16:15,790 --> 00:16:20,230 +طلبناهم قولين في laptop و التاني اتنين من اربع، + +228 +00:16:20,230 --> 00:16:26,990 +واحد من اربع كمان، لكن للأسف مالاجيناش laptop واحد + +229 +00:16:26,990 --> 00:16:31,170 +اللي فيه الأحلام تبعتنا، اللي بدنا هيها احنا في + +230 +00:16:31,170 --> 00:16:35,320 +المواصفاتفبنتضطر إنه إذا أنا بحتاج هذه المواصفات + +231 +00:16:35,320 --> 00:16:37,680 +إن أنا أشتري لابتوب هذا و أشتري لابتوب هذا و أشتري + +232 +00:16:37,680 --> 00:16:41,580 +لابتوب هذا إذا بستخدمه الأغراض متنوعة و مختلفة + +233 +00:16:41,580 --> 00:16:45,280 +وهذا اللي هي بيصير بالفعل في المختبرات ال ideal + +234 +00:16:45,280 --> 00:16:50,200 +antiseptic أو disinfectant موصل نهر يعني it must + +235 +00:16:50,200 --> 00:16:54,080 +be cheap لازم يكون رخيص it must be effective at + +236 +00:16:54,080 --> 00:16:58,960 +low concentration it must be safe مافيش irritation + +237 +00:16:58,960 --> 00:17:02,430 +مالهوش رائحةكريهة على الأقل بيروح المستشفى + +238 +00:17:02,430 --> 00:17:06,070 +بيلاقيهم ناطفيه الأرضيات بكلور ريحت المستشفى تكون + +239 +00:17:06,070 --> 00:17:09,490 +سيئة للغاية صح دلوقت و قد تؤذي المريض بعض الناس + +240 +00:17:09,490 --> 00:17:13,910 +هيتحسسوا من ال .. في مواد قاتلة جدا للبكتيريا + +241 +00:17:13,910 --> 00:17:18,750 +وسامة للإنسان فبالتالي المواصفات الميثالية كمان + +242 +00:17:18,750 --> 00:17:22,750 +مرة اللي احنا بيزنيها مش متوفرة في agent واحد لكن + +243 +00:17:22,750 --> 00:17:26,170 +ممكن نلاقي في هذا ال agent خمسة من عشرة ستة من + +244 +00:17:26,170 --> 00:17:29,230 +عشرة سبعة من عشرة و بالتالي بنستخدم الأجراض محددة + +245 +00:17:35,560 --> 00:17:39,040 +أحنا قلنا راح نقسم العوامل ل chemical and physical + +246 +00:17:39,040 --> 00:17:47,060 +agents بس في نقطة كتير قبل نقمز عنها في اللي لو + +247 +00:17:47,060 --> 00:17:53,580 +حصلها لقها احنا التعريف الطبي للموت عند البشر توقف + +248 +00:17:53,580 --> 00:17:55,200 +القلب او توقف الدماء + +249 +00:17:59,210 --> 00:18:05,750 +بيعد إلى الموت؟ بس بس بس بس بس بس بس بس بس بس بس + +250 +00:18:05,750 --> 00:18:10,870 +بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس + +251 +00:18:10,870 --> 00:18:13,590 +بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس + +252 +00:18:13,590 --> 00:18:13,790 +بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس + +253 +00:18:13,790 --> 00:18:14,070 +بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس + +254 +00:18:14,070 --> 00:18:14,230 +بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس بس + +255 +00:18:14,230 --> 00:18:20,190 +بس بس بس بس بس بس بس بس بس بس بس بس بس + +256 +00:18:20,790 --> 00:18:24,450 +Death is defined by the inability to reproduce + +257 +00:18:24,450 --> 00:18:29,390 +مدام مش قادر الميكروبيت كاتر فهو ميت هذا لغرض + +258 +00:18:29,390 --> 00:18:36,290 +تعريف ال disinfection طيب في نقطة كتير مهمة الان + +259 +00:18:36,290 --> 00:18:40,970 +لو أجى واحد و جالنا عرض علينا antiseptic جديد او + +260 +00:18:40,970 --> 00:18:45,290 +disinfectant جديد ال disinfectant هذا بدنا نقارنه + +261 +00:18:45,290 --> 00:18:50,100 +بدنا نشوف فعاليته هل هو فعال او مش فعالبنفعش دايما + +262 +00:18:50,100 --> 00:18:55,440 +كلمة انقارن بدها اتنين، بدها اتنين، على الأقل بدنا + +263 +00:18:55,440 --> 00:18:59,460 +واحد معيار و بدنا واحد انقارنه في المعيار، صح؟ + +264 +00:18:59,460 --> 00:19:03,860 +المعيار اللي مورود عندنا اسمه فينول الفينول أحد + +265 +00:19:03,860 --> 00:19:10,300 +المركبات الأولى + +266 +00:19:10,300 --> 00:19:13,140 +اللي استخدمت في عمليات التطهير و disinfection + +267 +00:19:13,140 --> 00:19:18,540 +وبالتالي أي مركب جديد بنقارنه مين؟ بالفينولتعالى + +268 +00:19:18,540 --> 00:19:22,960 +بس لاني بلغة بسيطة عشان مش مجان نشرح بالضبط تفاصيل + +269 +00:19:22,960 --> 00:19:27,900 +كيفية العملية بالتم لكن نشرح الفكرة الان الفينول + +270 +00:19:27,900 --> 00:19:35,740 +عند عين تركيز X بيقتل و زمان Y بيقتل عشرة أس ستة + +271 +00:19:35,740 --> 00:19:39,980 +من البكتيريا تمام؟ المادة الجديدة اللى بدي إياها + +272 +00:19:39,980 --> 00:19:46,240 +أنا المادة الجديدة اللى بدي إياها بدي أعرف at what + +273 +00:19:46,240 --> 00:19:55,190 +concentrationat what time؟ at the same time at the + +274 +00:19:55,190 --> 00:19:59,710 +same time الناس راح تبقى هنا و الكل عشرة او ستة + +275 +00:19:59,710 --> 00:20:06,490 +ايش اللي fixed هنا؟ الزمن؟ الزمن صح؟ و عادل + +276 +00:20:06,490 --> 00:20:09,630 +البكتيريا؟ مين ضلحت هنا المضايقات؟ اللي هو ال + +277 +00:20:09,630 --> 00:20:13,310 +concentration ال phenol coefficient هو عبارة عن ال + +278 +00:20:13,310 --> 00:20:18,950 +concentration of phenolعلى ال concentration of ال + +279 +00:20:18,950 --> 00:20:25,310 +agent X العمل الجديد هذا تخيلوا + +280 +00:20:25,310 --> 00:20:30,730 +لو ال concentration طلعت 20 و أنا عشرين تمام؟ ايش + +281 +00:20:30,730 --> 00:20:35,070 +بتقول ال coefficient؟ واحد ايش معنى الحكاية؟ انه + +282 +00:20:35,070 --> 00:20:41,930 +العامل هذا الجديد اللي هو X is comparable نقارنه + +283 +00:20:41,930 --> 00:20:45,450 +بمين؟ بالفنون يعني نفس الكفاءة مع مين؟ مع الفنون + +284 +00:20:46,910 --> 00:20:55,090 +point واحد ايه ال coefficient؟ اقل او اكتر؟ احسن + +285 +00:20:55,090 --> 00:21:03,110 +او اقل؟ احسن او اقل؟ احسن او اقل؟ احسن او اقل؟ + +286 +00:21:03,110 --> 00:21:04,830 +احسن او اقل؟ احسن او اقل؟ احسن او اقل؟ احسن او + +287 +00:21:04,830 --> 00:21:05,850 +اقل؟ احسن او اقل؟ احسن او اقل؟ احسن او اقل؟ احسن + +288 +00:21:05,850 --> 00:21:09,310 +او اقل؟ احسن او اقل؟ احسن او اقل؟ احسن او اقل؟ + +289 +00:21:09,310 --> 00:21:09,350 +احسن او اقل؟ احسن او اقل؟ احسن او اقل؟ احسن او + +290 +00:21:09,350 --> 00:21:18,090 +اقل؟ احسن او اقل؟ احسن االعمل الجديد، لما نقول + +291 +00:21:18,090 --> 00:21:22,870 +تركيز أعلى، يعني كفاءة أقل، يبقى لما يكون ال point + +292 +00:21:22,870 --> 00:21:25,630 +.. لما يكون ال coefficient أقل من واحد، يكون + +293 +00:21:25,630 --> 00:21:29,810 +العامل أقل من كفاءة main الفنوة، إذا كان أكثر من + +294 +00:21:29,810 --> 00:21:34,230 +واحد العامل تمام، مانا الحاجة إنه الكفاءة الصالح + +295 +00:21:34,230 --> 00:21:35,850 +للعمل ال major + +296 +00:21:45,110 --> 00:21:50,590 +مهم جدا لأنه بيجينا كتير مراكبات باستمرار احنا + +297 +00:21:50,590 --> 00:21:56,190 +لازم نحدد فعاليتها عن طريق مقارنة بمين بالفينول + +298 +00:21:56,190 --> 00:22:00,430 +ويسمى فينول coefficient معامل الفينول + +299 +00:22:09,870 --> 00:22:12,610 +بقولنا الـ quantitative measure of this variation + +300 +00:22:12,610 --> 00:22:15,750 +is expressed as the phenol coefficient الكلام اللي + +301 +00:22:15,750 --> 00:22:18,110 +حكيناه جاب شويه الـ phenol coefficient اللي هو + +302 +00:22:18,110 --> 00:22:24,330 +معامل الـ phenol لماذا إلجأنا لفحص أو لعمل أو + +303 +00:22:24,330 --> 00:22:28,930 +لتحديد الـ phenol coefficient بغرض المقارنة عشان + +304 +00:22:28,930 --> 00:22:32,410 +نحدد ال variation هحدد اللي هو ال variation مهم + +305 +00:22:32,410 --> 00:22:35,110 +جدا نحدد ال variation عشان نحدد ال concentration + +306 +00:22:35,110 --> 00:22:40,190 +اللي بدي أستخدمها و ال time اللي أنا حاملهExposure + +307 +00:22:40,190 --> 00:22:46,290 +للأسطح اللي أنا بدي طهرها أو بدي أعقب هناك ثلاثة + +308 +00:22:46,290 --> 00:22:51,650 +آليات مشهورة جدا في ال disinfectant و ال + +309 +00:22:51,650 --> 00:22:55,130 +antiseptics wherein the agent may kill the + +310 +00:22:55,130 --> 00:22:58,330 +microorganism الأولى by cell membrane damage + +311 +00:22:58,330 --> 00:23:02,130 +التانية by protein denaturation تلاتة by DNA + +312 +00:23:02,130 --> 00:23:05,690 +damage أو modification يعني واحدة من تلاتة يا إما + +313 +00:23:05,690 --> 00:23:08,230 +على ال cell membraneأو على الـ membrane أو على الـ + +314 +00:23:08,230 --> 00:23:12,050 +phospholipid يما على البروتيني انه .. لكن بدكوا + +315 +00:23:12,050 --> 00:23:16,210 +تلاحظوا نقطة كتير مهمة في هذا الجانب مختلفة عن ال + +316 +00:23:16,210 --> 00:23:20,050 +antibiotics ال antibiotics .. ال antibiotics + +317 +00:23:20,050 --> 00:23:26,370 +بتشتغل على خطوة واحدة very very specificالانتيبايت + +318 +00:23:26,370 --> 00:23:30,790 +بيشتغل عليها very specific step يعني مثلا ابترتبط + +319 +00:23:30,790 --> 00:23:35,810 +بال 50S-ribosomal subunit وبتمنع ال translocation + +320 +00:23:35,810 --> 00:23:39,670 +لل amino acids الجديدة وبالتالي بتوقف تصنيع ال + +321 +00:23:39,670 --> 00:23:43,270 +protein بينما هذا اللي هتقولك modification of + +322 +00:23:43,270 --> 00:23:49,750 +proteins اي protein بيخربه اي DNA بيخربه وبالتالي + +323 +00:23:49,750 --> 00:23:53,610 +بدنا نفهم ان الميكانيزم هنا مش كتير specific + +324 +00:23:54,410 --> 00:23:58,470 +بالإضافة إلى أن بعض المُركّبات قد تستطيع قتل + +325 +00:23:58,470 --> 00:24:03,790 +البكتيريا من خلال أكثر من آلية معينة وهذا مصطلح مش + +326 +00:24:03,790 --> 00:24:09,670 +مذكور معانا الآن، بدنا نكتبه اسمه الـ Oligodynamic + +327 +00:24:09,670 --> 00:24:15,590 +Action قدرنا + +328 +00:24:15,590 --> 00:24:20,930 +نكون فهم، يعني الـ agent هو واحد قادر على قتل + +329 +00:24:21,490 --> 00:24:25,550 +البكتيريا بأكثر من وسيلة مثلا بيعمل disruption للـ + +330 +00:24:25,550 --> 00:24:27,810 +lipid membrane وبنفس الوقت بيعمل protein + +331 +00:24:27,810 --> 00:24:33,630 +denaturation إيش سمينا هذا العامل؟ الريباندركتشون + +332 +00:24:33,630 --> 00:24:37,610 +ليش أنا حابب اتأكد على هذه المعلومة و أذكركم إن ال + +333 +00:24:37,610 --> 00:24:42,450 +antibiotic specific البكتيريا استطاعت بنجاح إنها + +334 +00:24:42,450 --> 00:24:46,490 +تكون resistance لكثير من ال antibiotic ليش؟ لأن ال + +335 +00:24:46,490 --> 00:24:50,750 +antibiotic بيأثر على one stepفلو صار ال mutation + +336 +00:24:50,750 --> 00:24:53,850 +فاكرين ال mutations المحاضرة السابقة؟ لو صار ال + +337 +00:24:53,850 --> 00:24:57,210 +mutation أو it acquired a gene of resistance + +338 +00:24:57,210 --> 00:25:00,730 +البكتيريا صارت resistant لمين؟ لل antibiotic لكن + +339 +00:25:00,730 --> 00:25:05,070 +لحد الآن كده استخدامنا ال detol تمام ال resistant + +340 +00:25:05,070 --> 00:25:08,550 +لل detol يكاد لا يذكر ال resistant لل chlorine + +341 +00:25:08,550 --> 00:25:12,670 +اللي هو ال chlorine يكاد لا يذكر لأنه جالبا هو ال + +342 +00:25:12,670 --> 00:25:16,010 +mechanism هي non specific و في نفس الوقت قد تكون + +343 +00:25:16,010 --> 00:25:17,770 +ايه؟ Oligodynamic + +344 +00:25:21,210 --> 00:25:23,690 +من المُركّبات اللي بتعمل disruption لـ cell + +345 +00:25:23,690 --> 00:25:29,730 +membrane الكحول المشهور بالـ Ethanol وفي اسم تاني + +346 +00:25:29,730 --> 00:25:32,550 +اللي لسه مش متقوله ان احنا هتذكره كمان شوية اللي + +347 +00:25:32,550 --> 00:25:37,930 +هو الـ isopropanol وفيه احنا الـ detergent هو + +348 +00:25:37,930 --> 00:25:42,770 +متنميز ال detergent ليه المنظفات وقد يتواضر للذهن + +349 +00:25:42,770 --> 00:25:46,110 +بعض الناس اللي بيقولون منظفات انه يخطر على باله + +350 +00:25:46,110 --> 00:25:51,040 +الصابون لأ الصابون ليس له علاقة بالمنظفاتو بتسمعوا + +351 +00:25:51,040 --> 00:25:54,880 +انتوا بعض اللي أحيان في الدعايات صابون متحر صابون + +352 +00:25:54,880 --> 00:25:59,720 +Detol على سبيل المثال و يقتل 99.9% من الجراثيم + +353 +00:25:59,720 --> 00:26:05,860 +طبعا هو بيقتل 100% بس ال 99.9% هذي بيحطوها كمرة + +354 +00:26:05,860 --> 00:26:09,160 +احنا في ال micro biology غالبا لا نتداعى الدقة + +355 +00:26:09,160 --> 00:26:14,400 +لإنه طريقة تفكيرنا مبنية على assumptions على + +356 +00:26:14,400 --> 00:26:18,250 +افتراضات ده بيجعلوا يشوفوا ان الموتيعرف بإنه عدم + +357 +00:26:18,250 --> 00:26:21,110 +القدرة على البكتيريا لتعمل reproduction لكن لو أنا + +358 +00:26:21,110 --> 00:26:25,050 +أخدت البكتيريا التعبانة اللي هي غير قادرة على ال + +359 +00:26:25,050 --> 00:26:28,770 +reproduction و حطيتها في enrichment media قد تعود + +360 +00:26:28,770 --> 00:26:33,350 +يعني قد نحييها مرة أخرى بالتعريف اللي هو الخاطأ + +361 +00:26:33,350 --> 00:26:36,130 +اللي موجود في ال assumption مش دقيقة وبالتالي + +362 +00:26:36,130 --> 00:26:40,690 +بنحاول قدر الإمكان إنه ما نعطي أيضا كمان أرقام + +363 +00:26:40,690 --> 00:26:45,700 +دقيقة لما قلنا لكوا PFUblack forming unit مابنهاش + +364 +00:26:45,700 --> 00:26:49,700 +بكتيريوفاج و لما نتعدى البكتيريا بنقول cfu colony + +365 +00:26:49,700 --> 00:26:53,300 +forming unit عشان برضه مانفترضش أننا بنعدى بكتيريا + +366 +00:26:53,300 --> 00:26:58,880 +و أننا بنعدى وحدة بناء مستمرة ال detergent غير عن + +367 +00:26:58,880 --> 00:27:05,060 +الصابون الصابون العادى اللى احنا بنقولكه اغسل يديك + +368 +00:27:05,060 --> 00:27:09,260 +بالماء والصابون باستمرار و نصيحة و موجودة في + +369 +00:27:09,260 --> 00:27:14,550 +المنزلوموجودة حتى في أكتر الأماكن يعني احتياجة + +370 +00:27:14,550 --> 00:27:17,970 +للتطهير والتعقيم، اللي هي المستشفيات أو العبادة + +371 +00:27:17,970 --> 00:27:23,690 +الجراحية، إذن هو ليس متهر الصابون وإنما .. وإنما + +372 +00:27:23,690 --> 00:27:27,410 +.. لأ مش منظف كمان، خلّيني أشرح هو، لأن كلمة منظف + +373 +00:27:27,410 --> 00:27:31,870 +هي مصطلح يطلق على مين؟ على الـ detergent، هي تقوم + +374 +00:27:31,870 --> 00:27:34,770 +بـmechanical removal of the microorganism، مش أنه + +375 +00:27:34,770 --> 00:27:38,250 +mechanicalيعني بتساعد على أنه البكتيرى اللى على + +376 +00:27:38,250 --> 00:27:43,750 +إيدينا تنزل تروح تمام بس مابيقتلهاش الصوان بينما + +377 +00:27:43,750 --> 00:27:47,650 +ال detergent ممكن يساعد في إزالتها وبيقتلها في نفس + +378 +00:27:47,650 --> 00:27:50,450 +الوجه، فب .. هذا مختلف يعني ال detergent غير عن + +379 +00:27:50,450 --> 00:27:53,170 +الصوان بالإضافة إلى ال phenols اللى أحنا حاكينا + +380 +00:27:53,170 --> 00:27:57,790 +عنهم إنه من أقدم المركبات المستخدمة في التطبيق نجل + +381 +00:27:57,790 --> 00:28:02,950 +ال EthanolEthanol is widely used to clean the skin + +382 +00:28:02,950 --> 00:28:07,270 +before immunization or venipuncture طبعا بتعرفين + +383 +00:28:07,270 --> 00:28:10,510 +اللي لما بناخدنا الطعام بمسحوناه في جطنه و باخدونا + +384 +00:28:10,510 --> 00:28:15,150 +القبره او لما بنسحب عين الدم بس الان بنتكلم على + +385 +00:28:15,150 --> 00:28:18,970 +سحب عين الدم في ال micro biology بتختلف عن سحب عين + +386 +00:28:18,970 --> 00:28:21,470 +الدم في ال hematology او ال chemical chemistry + +387 +00:28:21,470 --> 00:28:29,890 +الكحول ال 70% متحر والتطهير ليسabsolute killing + +388 +00:28:29,890 --> 00:28:34,490 +صح؟ اللي هو مش ده sterilization أنا بدي أحاول أعمل + +389 +00:28:34,490 --> 00:28:38,610 +ما يشبه ال sterilization في ال skin لما بدي أعمل + +390 +00:28:38,610 --> 00:28:41,530 +blood culture عارفين ليش؟ لإن بالضبط من إن ال + +391 +00:28:41,530 --> 00:28:45,190 +bacteria نمت معي في المختبر نمت معي من الدم و ليس + +392 +00:28:45,190 --> 00:28:49,330 +من مين؟ من الجلد لإن الجلد لما احنا دخلنا إلى الدم + +393 +00:28:49,330 --> 00:28:54,130 +دخلنا و أخدنا دم لكن عبر مين؟ الجلد فإذا كان في + +394 +00:28:54,130 --> 00:28:58,710 +بكتيريا على الجلد هترجع معاها هذا واحدوخدوا بألكوا + +395 +00:28:58,710 --> 00:29:02,650 +الأصل إن العملية هذه تتم حتى في سحب الدم بطريقة + +396 +00:29:02,650 --> 00:29:08,710 +روتينية لإنه ممكن ندخل أصلا بكتيريا إلى الدم، الآن + +397 +00:29:08,710 --> 00:29:14,570 +عشان نلجأ لهذه العملية، بنضطر نعمل عملية إضافية أو + +398 +00:29:14,570 --> 00:29:19,430 +عملتين إضافيتين، كحول نمسح فيه وعارفين الطريقة من + +399 +00:29:19,430 --> 00:29:24,430 +ال center لل outward طريقة الأولابية من المركز إلى + +400 +00:29:24,430 --> 00:29:28,880 +اللي هو قلنا إيه إلى الخارجمش من فوق لتحت أو من + +401 +00:29:28,880 --> 00:29:34,140 +تحت لفوق يعني إذا كان في أوساخ أوساخ موجودة ننزلها + +402 +00:29:34,140 --> 00:29:38,400 +تحت و بعدين نرفعها فوق و كأنك ما عملتيشي كأنك + +403 +00:29:38,400 --> 00:29:42,420 +ماعملتيشي هيجب أن تكون بشكل دائري لو اللابي بهذا + +404 +00:29:42,420 --> 00:29:48,520 +الشكل هذا رقم واحد رقم اتنين بنضيف .. بنضيف اللي + +405 +00:29:48,520 --> 00:29:55,220 +هو ال أيودين صبغة اليود و بنتركها على اليد لمدة + +406 +00:29:55,220 --> 00:30:02,130 +دقيقتينوبعد ما يخلصوا الدقيقتين بنروح واحنا شايلين + +407 +00:30:02,130 --> 00:30:07,650 +الأيودين بقطنة محتوية على 70% الكحول، يبقى كحول، + +408 +00:30:07,650 --> 00:30:12,530 +أيودين، وبعدين كحول، هذا بيضمن لإن الأيودين عنده + +409 +00:30:12,530 --> 00:30:18,000 +قدرة قتل أكبر بكثير من مين من الكحولبيضمنلي انه + +410 +00:30:18,000 --> 00:30:21,680 +يعني بنسبة تسعة وتسعين بالمئة نجمي مول اكتر من ذلك + +411 +00:30:21,680 --> 00:30:24,580 +انه مافيش ضايق لإيه مايكرو أورجينزم لإن الأيودين + +412 +00:30:24,580 --> 00:30:29,340 +مشهور عنه قدرته على انه بسموه ده germicidal دلوقت + +413 +00:30:29,340 --> 00:30:32,900 +انه بيقتل البكتيريا و الفطريات و الفيروسات حتى ال + +414 +00:30:32,900 --> 00:30:37,540 +sports فهو فريد من مين؟ من إنه يكون sterilant مادة + +415 +00:30:37,540 --> 00:30:43,880 +معاقبةأعتقد إن إحنا حكينا عن ال 70% و عن ال 100% و + +416 +00:30:43,880 --> 00:30:48,420 +كنا إن ال 100% يرفع أعلى لسبب + +417 +00:30:50,870 --> 00:30:53,210 +لأ انا بالدولة عاوزة تفضل اللي بتديله مش هو انا + +418 +00:30:53,210 --> 00:30:57,950 +لما زيد الزمن زيدي التركيز بيزيد الزمن؟ لأ عكس هذا + +419 +00:30:57,950 --> 00:31:01,590 +الاستثناء و هذا الاستثناء بالعكس لما يزيد التركيز + +420 +00:31:01,590 --> 00:31:07,290 +بيقل الزمن طب كيف انه هي العلاقة أن تتناسب ترضي مع + +421 +00:31:07,290 --> 00:31:10,730 +سيء في team مش ترضي مش ترضي عكس طب ماجي لكن + +422 +00:31:10,730 --> 00:31:16,090 +التركيز و الزمن ترضي بين بعض عشانالتركيز و الزمن + +423 +00:31:16,090 --> 00:31:19,390 +.. و الزمن .. طرد .. مش قولنا كل ما زادت التركيز + +424 +00:31:19,390 --> 00:31:22,470 +.. لأ يا بنتي لأ لأ لأ .. كل ما زادت التركيز قل + +425 +00:31:22,470 --> 00:31:26,250 +الزمن .. قل الزمن، هذا علاقة غير علاقة الأولانية، + +426 +00:31:26,250 --> 00:31:28,870 +علاقة الأولانية كانت number of survivors، فتناسى + +427 +00:31:28,870 --> 00:31:33,770 +الترسم عكسيا مع العاملين مع بعض، مضلوبين في بعض، + +428 +00:31:33,770 --> 00:31:36,770 +التناسي مع عكس طبعا .. the more .. the more .. the + +429 +00:31:36,770 --> 00:31:39,270 +higher the concentration, the less the time is + +430 +00:31:39,270 --> 00:31:44,290 +required، يعني علق عكسيالكن هذه الاستثناء قلنا انه + +431 +00:31:44,290 --> 00:31:48,830 +ال concentration لحد معين كل ما ارتفعنا كل ما زادت + +432 +00:31:48,830 --> 00:31:52,130 +الكفاءة كل ما زادت الكفاءة لكن لكن هذا ان المائة + +433 +00:31:52,130 --> 00:31:57,470 +بالمائة من المركب كفائتها منخفضة ايش السبب؟ ان + +434 +00:31:57,470 --> 00:32:01,430 +الكهوية كان يعمل محتاج ماء مائة بالمائة كهوية + +435 +00:32:01,430 --> 00:32:06,710 +مافيش ميه قدرته على ال penetration بتكون اقل وإذا + +436 +00:32:06,710 --> 00:32:10,470 +ما اخترقش داخل الخلية مانحك مش هعمل destruction + +437 +00:32:10,470 --> 00:32:14,250 +للمنبرينولا ال proteins هو بيشتغل بالطريقتين طريقة + +438 +00:32:14,250 --> 00:32:18,070 +انه بيعمل disorganization ل ال membrane lipid و + +439 +00:32:18,070 --> 00:32:21,550 +بيعمل denaturation لمن؟ ل ال proteins إذا هو من + +440 +00:32:21,550 --> 00:32:25,510 +العوامل اللي بتشتغل oligodynamic action لذلك أنا + +441 +00:32:25,510 --> 00:32:30,110 +لحد الآن لم أقرا بكتيريا مقاومة طبعا يعني كانت + +442 +00:32:30,110 --> 00:32:34,490 +sensitive قصارة مقاومة أنا مبسوط فيها للكحول لإن + +443 +00:32:34,490 --> 00:32:38,170 +الكحول جدنا بيقضي عليها بطوله non specific لكن + +444 +00:32:38,170 --> 00:32:43,540 +تخيلوا لو بيشتغل على one step فقطفيه chance .. فيه + +445 +00:32:43,540 --> 00:32:45,760 +chance عالية أن البكتيريا تكون له resistance + +446 +00:32:45,760 --> 00:32:49,680 +بتعطيكوا مثال بس يعني خارج الإطار عشان برضه الصورة + +447 +00:32:49,680 --> 00:32:55,340 +تُضح بشكل جيد هناك حالة بنتسميها Combined Therapy + +448 +00:32:55,340 --> 00:32:59,540 +علاج مشترك بمعنى أنه مانعطيش Antibiotic، مانعطي 2 + +449 +00:32:59,540 --> 00:33:02,160 +أو 3 أو 4 Antibiotics يعني في مثلا الـ + +450 +00:33:02,160 --> 00:33:05,160 +Tuberculosis، مانعطيله أربع أنواع من المضاد + +451 +00:33:05,160 --> 00:33:11,500 +الحيوية تعني نسميهم ألف A وB وC وD وهي البكتيريا + +452 +00:33:12,500 --> 00:33:18,920 +اللي هي Mycobacterium Tuberculosis الآن لو كوّنت + +453 +00:33:18,920 --> 00:33:29,880 +resistant لإيه؟ B و C و D يقتلوها، صح؟ يبقى + +454 +00:33:29,880 --> 00:33:34,480 +البكتيريا اللي كوّنت resistant لإيه؟ ظهرت على + +455 +00:33:34,480 --> 00:33:38,740 +العلم؟ ولا ماتت في مهدها؟ قُتلت في مهدها، لم تنشأ + +456 +00:33:38,740 --> 00:33:43,770 +منها سلولات لأنه قتلت .. احنا قطيهم مع بعضطيب لو + +457 +00:33:43,770 --> 00:33:52,690 +جللنا ال antibiotic وصحيح ان a,b,c بالان كومة + +458 +00:33:52,690 --> 00:33:58,610 +resistant ل a قتلها مين؟ b و c لكن احتمالية ان + +459 +00:33:58,610 --> 00:34:05,370 +البكتيريا تكون resistant ل b و c في وجود واحد رابع + +460 +00:34:05,370 --> 00:34:11,370 +او خامس او سادس بتكون عايشة اقل فكل ما زال عد ال + +461 +00:34:11,370 --> 00:34:14,830 +antibioticsاللي انا بدي افترض ان كل انتيباتك له + +462 +00:34:14,830 --> 00:34:19,450 +ميكانيزم of action فلما يكون ال disinfectant بيقتل + +463 +00:34:19,450 --> 00:34:23,290 +البكتيريا ب different mechanisms لما لو قورت + +464 +00:34:23,290 --> 00:34:26,890 +resistant لميكانيزم هيقتلها بميكانيزم أخرى + +465 +00:34:26,890 --> 00:34:30,990 +وبالتالي لن تظهر إلى العالم و تبقى في السوق يعني + +466 +00:34:30,990 --> 00:34:35,830 +في الدنيا و بتموت في مهدها و تلصورها فكويس جدا + +467 +00:34:35,830 --> 00:34:38,030 +يكون عندي حاسب ما اقول لكوا dynamic action + +468 +00:34:42,270 --> 00:34:46,730 +من طبعا الكحولات المشهورة جدا في العالم بحاجة + +469 +00:34:46,730 --> 00:34:50,530 +اسمها isopropanoid بذكر الأسفلات اللي كتبوها ايزو + +470 +00:34:50,530 --> 00:34:54,490 +برضه + +471 +00:34:54,490 --> 00:34:59,030 +بيستخدموه بتركيز 70% من ميزاته انه بيحافظ على + +472 +00:34:59,030 --> 00:35:04,550 +رطوبة الجلد اكثر من ال Ethanol خدوا بالكم النقطة + +473 +00:35:04,550 --> 00:35:10,310 +هانفي التعامل في المستشفيات احنا بننصح انه بين كل + +474 +00:35:10,310 --> 00:35:16,350 +مريض و مريض مثلا الطبيب انه يطهر ايديه تطهير + +475 +00:35:16,350 --> 00:35:19,750 +الأيدان بين .. لو بشوفله عشرين تلاتين مريض يعني + +476 +00:35:19,750 --> 00:35:24,150 +مادي حاجة هيغسل ايديه عشرين مرة ما بيخلص اخر نهار + +477 +00:35:24,150 --> 00:35:28,650 +اللي هو جلده ناشف متشقق بصير هو عرض اصلا للاتهابات + +478 +00:35:28,650 --> 00:35:33,710 +الشخص هذا للطبيب او ال nurse وبالتالي تطهير + +479 +00:35:33,710 --> 00:35:38,340 +والتعقيم أنتج مشكلة جديدةفاضطرينا نحلها بإنه + +480 +00:35:38,340 --> 00:35:45,260 +نستخدم مراكبات أقل حدة على الجل مثلا لازربربنال + +481 +00:35:45,260 --> 00:35:50,100 +واحد منهم أو في هسمة alcohol gel ال alcohol gel + +482 +00:35:50,100 --> 00:35:55,840 +بيستخدموه متعالي اللي بيكون عبارة عن جل و نحطه على + +483 +00:35:55,840 --> 00:36:02,360 +جل و بعدين هذا بيقلل من اللي هو فقدان الرطوبة في + +484 +00:36:02,360 --> 00:36:07,770 +الإيدين وبالتالي بيحافظ على الإيدينرطبة وليس جياب + +485 +00:36:07,770 --> 00:36:12,950 +رقم + +486 +00:36:12,950 --> 00:36:16,030 +اتنين detergent رقم اتنين هو ال detergent و ال + +487 +00:36:16,030 --> 00:36:19,270 +detergent كما وصفته انا و ليس الصابون و انما هو + +488 +00:36:19,270 --> 00:36:25,070 +يبقى surface active agent عامل له نشاط سطحي و + +489 +00:36:25,070 --> 00:36:27,590 +بيسموه surfactant اللي هو المصطلح اللي هنا + +490 +00:36:27,590 --> 00:36:33,610 +surfactant تمام؟ بيمتلك hydrophilicو hydrophobic + +491 +00:36:33,610 --> 00:36:38,710 +ends مين من المركبات اللي درسناها في البكتيريا + +492 +00:36:38,710 --> 00:36:42,150 +عندها hydrophilic و hydrophobic end؟ ال cell + +493 +00:36:42,150 --> 00:36:45,890 +membrane و ال outer membrane تبع ال gram negative + +494 +00:36:45,890 --> 00:36:52,350 +بكتيريا، مضطر لأ؟ طيب لما ييجي surfactant إله + +495 +00:36:52,350 --> 00:36:57,770 +hydrophobic end و يتفاعل مع ال hydrophilic end تبع + +496 +00:36:57,770 --> 00:37:02,460 +ال cell membraneمين أقوى ال surfactant ولا ال cell + +497 +00:37:02,460 --> 00:37:09,100 +membrane فبروح اللي هو شادد ممزق ال cell membrane + +498 +00:37:09,100 --> 00:37:12,480 +فاكرين ال cell membrane كيف وصفناها لما وصفنا زيت + +499 +00:37:12,480 --> 00:37:20,200 +زيتون يعني لزج جدا مرن جدا رقيق جدا طبعا صلاته + +500 +00:37:20,200 --> 00:37:25,320 +ضعيفة جدا وبالتالي من السهل تمزيقه لكن بتلاعب ان + +501 +00:37:25,320 --> 00:37:31,470 +ال surfactant بيشتغلو احنا مش قولنا بدمر الcell + +502 +00:37:31,470 --> 00:37:36,210 +membrane عبر مين؟ عبر ال hydrophobic chain و + +503 +00:37:36,210 --> 00:37:40,570 +بيرتبط مع الميه في المحيط عبر ال hydrophobic chain + +504 +00:37:40,570 --> 00:37:44,590 +فبيقتل البكتيريا و ممكن يزيلها بمعنى اخر زي ما + +505 +00:37:44,590 --> 00:37:48,070 +بيشتغل مع الأوساخ، بيرتبط فيها و بيسحبها + +506 +00:37:50,720 --> 00:37:54,380 +من أشهر المركبات المشهورة حاجة اسمها Benzyl Konium + +507 +00:37:54,380 --> 00:37:59,000 +Chloride وهو يستخدم ك mild .. mild disinfectant أو + +508 +00:37:59,000 --> 00:38:02,420 +skin disinfectant وهو يتبع إلى ما يسمى بال + +509 +00:38:02,420 --> 00:38:06,320 +Quaternary Ammonium Sources Quaternary Ammonium + +510 +00:38:06,320 --> 00:38:10,260 +Compounds أو Quaternary Ammonium Source Benzyl + +511 +00:38:10,260 --> 00:38:15,760 +Konium استخدمت كايش ك skin العملية اللي بيسميها + +512 +00:38:15,760 --> 00:38:17,360 +disinfection + +513 +00:38:19,840 --> 00:38:22,640 +المُركّب التالف في السلسلة اللي هو الفينول + +514 +00:38:22,640 --> 00:38:26,560 +والفينول زي ما حكيتها لك هو ال first disinfectant + +515 +00:38:26,560 --> 00:38:30,620 +used in the operating room by Joseph Lister في ال + +516 +00:38:30,620 --> 00:38:36,720 +1860 أو ستينات القرن التاسع عشر تمانيات القرن .. + +517 +00:38:36,720 --> 00:38:41,320 +عفوا تمانيات القرن التاسع عشر Joseph Lister أُعطى + +518 +00:38:41,320 --> 00:38:45,080 +ال credit كله أُعطى يعني ال .. ال .. انه .. ان هو + +519 +00:38:45,080 --> 00:38:50,090 +صاحب التعقيم والتطهير في .. في المستشفياتلكن واقع + +520 +00:38:50,090 --> 00:38:54,650 +الانور انا لازم احكيلكوا قصة صغيرة عن احد الأطباء + +521 +00:38:54,650 --> 00:38:59,890 +في أوروبا اللي كان مهتم جدا في دراسة كتب الطب + +522 +00:38:59,890 --> 00:39:07,890 +الإسلامية بيقول انه نسخت الأدوات الجراحية من + +523 +00:39:07,890 --> 00:39:12,750 +الكتاب كما هي وعطيتها للمصنعين وصنعونا إياها يعني + +524 +00:39:12,750 --> 00:39:16,220 +مرسومة بدقة في الكتبو أعطاها لمثالين صنعوها و + +525 +00:39:16,220 --> 00:39:20,620 +أجريت العمليات الجراحية كما أجراها الطبيب الفلاني + +526 +00:39:20,620 --> 00:39:25,460 +اللي ذكر اسمه لأ أذكر اسمه أنا فيقول إنه نجحت + +527 +00:39:25,460 --> 00:39:28,700 +العملية و استطعت أن أجر العملية بالإنواد و بطريقة + +528 +00:39:28,700 --> 00:39:33,000 +تماما لكن المرض مات بعد العملية بانتفاخات و + +529 +00:39:33,000 --> 00:39:35,040 +انتهابات ماكانش طبعا أنهم معروفين شوية يعني + +530 +00:39:35,040 --> 00:39:38,720 +بكتيريا أيامها و هو بشكل واضح و تكررت العملية حتى + +531 +00:39:38,720 --> 00:39:41,880 +ضننتوا بيهم في ظنوم يعني إننا أعتقدت أن هدولة + +532 +00:39:41,880 --> 00:39:46,060 +كانوا يعني بتحكوا عينا في المعلومات اللي بعتوافيوم + +533 +00:39:46,060 --> 00:39:51,240 +يعني بيقرأ فاتلافت انتباهه انه أشياء ماكانش يركز + +534 +00:39:51,240 --> 00:39:53,780 +عليها زي ما احنا بعضنا حاببنا الزبدة بدناش + +535 +00:39:53,780 --> 00:40:00,920 +المقدمات و زي ما في كان نكتة بيقول انه اقطعي جزرة + +536 +00:40:00,920 --> 00:40:04,240 +وخيارة ومش عارف شو بتعمل طبيخ و بالآخر بيقولك ايه + +537 +00:40:04,240 --> 00:40:09,980 +بيقولك اغسل المكونات فهذا + +538 +00:40:09,980 --> 00:40:16,070 +عمل العملية عمل عملية كاملة كاملة بحد أثيرهالكن لم + +539 +00:40:16,070 --> 00:40:19,430 +يطبق إجراء كان بيقوم في الطي المسلم كونه مسلم كان + +540 +00:40:19,430 --> 00:40:24,550 +يتوضأ قبل إجراء العملية الوضوء قبل إجراء العملية + +541 +00:40:24,550 --> 00:40:28,050 +غسيل إيدان وغسيل إيدان عبارة عن تطهير تنظيف مظبوط + +542 +00:40:28,050 --> 00:40:31,670 +ولا لأ فجال طب بتجرب أنا الموضوع هتجرب الموضوع انه + +543 +00:40:31,670 --> 00:40:35,030 +صار يتوضأ وضوء اللي اللي بدي عليه بديه يمشي للآخر + +544 +00:40:35,030 --> 00:40:38,730 +و بالفعل بدأت الحالات عنده تتحسن يعني العمليات + +545 +00:40:38,730 --> 00:40:42,510 +الجراحية اللي بعد ذلك انه نسبة الوفيات فيها تقل + +546 +00:40:42,510 --> 00:40:46,660 +نظرا للانتهابات نبعت العمليةاللي حصل على credit + +547 +00:40:46,660 --> 00:40:50,820 +اللي هو Joseph Lester هو التاريخ اكتبوه الله يعني + +548 +00:40:50,820 --> 00:40:58,240 +هو المنبسطين هذا المُركّب .. هذا المُركّب دخل + +549 +00:40:58,240 --> 00:41:04,600 +المستشفيات لكن إله خواص سيّه سموه كاوية caustic + +550 +00:41:04,600 --> 00:41:08,340 +قابضة تمام؟ زي السودا الكاوية تمام؟ caustic soda + +551 +00:41:08,340 --> 00:41:13,420 +فهذا ال .. هذا ال .. الخاصية جاءت من استخدامه يعني + +552 +00:41:13,420 --> 00:41:17,860 +أمر غير مرضوب فيه خاصةبعد جهور مراكبات أخرى في + +553 +00:41:17,860 --> 00:41:22,240 +الخدمة أحد المراكبات اللي اشتقت دمجوا مراكبين + +554 +00:41:22,240 --> 00:41:29,520 +الفينول والكلور فهو يبقى هكسا كلورفين + +555 +00:41:29,520 --> 00:41:33,580 +كلورفين اللي هو كلورين مع فينول which is a + +556 +00:41:33,580 --> 00:41:36,980 +biphenol with six chlorine atoms is used in + +557 +00:41:36,980 --> 00:41:40,200 +germicidal soaps حطوها لما .. لما عملوا الصابون + +558 +00:41:40,200 --> 00:41:44,770 +ودخلوا الصابون لحالهكتركيب مش deserved ولا قاتل + +559 +00:41:44,770 --> 00:41:47,970 +للبكتيريا، لكن احنا بنشوف في ال .. في ال .. في ال + +560 +00:41:47,970 --> 00:41:51,710 +.. في السوق الآن أنه في germicidal soups يضافلها + +561 +00:41:51,710 --> 00:41:56,790 +مركبات تمام ليجعلها قاتلة، زي الـ Detol soup لكن + +562 +00:41:56,790 --> 00:42:01,210 +بقولنا .. بقولنا أنه هناك قلق من أنه إلو neuro + +563 +00:42:01,210 --> 00:42:06,270 +toxicity هذا المركب أظهر أو بعض الدراسات أظهرت أنه + +564 +00:42:06,270 --> 00:42:09,870 +ممكن يكون إلو neuro toxicity فأوقف برضه استخدام + +565 +00:42:10,540 --> 00:42:15,680 +أنشأ ومركّب جديد من الفينول another phenol derived + +566 +00:42:15,680 --> 00:42:21,500 +from this grisol وهو يبقى مثالفينول مثالفينول the + +567 +00:42:21,500 --> 00:42:24,880 +active ingredient in lysol اللي هو شبيه بالديترون + +568 +00:42:24,880 --> 00:42:30,140 +شبيه بالديترون فينولs not only damage membranes + +569 +00:42:30,140 --> 00:42:35,280 +but also damage من البروتين يبقى الأن أن مركبين + +570 +00:42:35,280 --> 00:42:40,150 +عرفناهم اللي همالفنولات والكحولات تمام عندهم نقطة + +571 +00:42:40,150 --> 00:42:43,150 +على ال destruction لل cell membrane بالإضافة إلى + +572 +00:42:43,150 --> 00:42:50,750 +ال proteins الآن بنجي للعنوان التاني في ال + +573 +00:42:50,750 --> 00:42:53,310 +mechanism of actions لل disinfectant و ال + +574 +00:42:53,310 --> 00:42:57,570 +antiseptic اللي هو ال denaturation أو ال + +575 +00:42:57,570 --> 00:43:00,510 +modification of proteins عندنا chlorine, iodine, + +576 +00:43:00,590 --> 00:43:02,690 +hedium metals, hydrogen peroxide عندنا + +577 +00:43:02,690 --> 00:43:06,450 +formaldehyde و glutaraldehydeEthylene Oxide اللي + +578 +00:43:06,450 --> 00:43:09,770 +مار معانا في أول المحاضرة لما نتكلم عن غاز + +579 +00:43:09,770 --> 00:43:14,370 +المستخدم في التعقيم وفي Acid and Alkali طبعا ال + +580 +00:43:14,370 --> 00:43:17,630 +Acid and Alkali ممكن تشتغل بأكتر من طريقة ال pH + +581 +00:43:17,630 --> 00:43:21,590 +نفسه يعمل suppression أو انه هو نفسه يكون عنده + +582 +00:43:21,590 --> 00:43:24,670 +mechanism of killing زي البنزوات ال sodium ان + +583 +00:43:24,670 --> 00:43:28,350 +تستخدمها كمادة حافظة في بعض المربيات المنزلية اللي + +584 +00:43:28,350 --> 00:43:32,110 +تمالوهاأو بعض المخللات المنزلية بتعملوا كرامة + +585 +00:43:32,110 --> 00:43:34,190 +الفيروس بتستخدم اللي هو ال Sodium Benzoate + +586 +00:43:34,190 --> 00:43:37,710 +وبيشتروها من الصيدليات كمادة بيقولوا عنها + +587 +00:43:37,710 --> 00:43:42,810 +البنزوات، همام؟ ال Sodium وهي مادة حافظة تنحل + +588 +00:43:42,810 --> 00:43:46,730 +وتكون Benzoic Acid، في الآخر هي إيه؟ عبارة عن + +589 +00:43:46,730 --> 00:43:54,110 +Acid، Organic Acid طيب، ال chlorine و ال Iodine + +590 +00:43:54,110 --> 00:44:00,600 +بينتموا إلى ال halogen's both haveيعني + +591 +00:44:00,600 --> 00:44:04,220 +antibacterial أو antimicrobial effect لكن + +592 +00:44:04,220 --> 00:44:08,920 +استخدامتهم مختلفة مثلا انتوا تشربوا الماء مخلوط + +593 +00:44:08,920 --> 00:44:12,360 +بمين؟ بالكلورين بس انتوا مش تشربوا الماء مخلوط + +594 +00:44:12,360 --> 00:44:15,800 +بالايودين صح ولا لأ؟ هذا يستخدم لغرض و هذا يستخدم + +595 +00:44:15,800 --> 00:44:21,100 +لأغراض مختلفة تعالوا نطلع على الكلورين كلورين is + +596 +00:44:21,100 --> 00:44:26,020 +used as a disinfectant to purify the water supply + +597 +00:44:26,020 --> 00:44:30,390 +and to treat swimming poolsأنتوا تعرفوا أن في قطاع + +598 +00:44:30,390 --> 00:44:34,730 +غزة we are blessed by a plenty of areas where + +599 +00:44:34,730 --> 00:44:38,770 +swimming pools are common يعني الآن صار فينا مناطق + +600 +00:44:38,770 --> 00:44:42,050 +متنوعة مختلفة حتى في بعض المنازل صار فيها swimming + +601 +00:44:42,050 --> 00:44:45,870 +pools اللي عنده قطعة أرض صغيرة بيعمله قط .. لكن + +602 +00:44:45,870 --> 00:44:51,790 +للأسف كثير من هؤلاء الناس ما .. مش فاهمين إيش يعني + +603 +00:44:51,790 --> 00:44:59,090 +swimming pooldifferent persons بيدخلوا المكان هذا + +604 +00:44:59,090 --> 00:45:04,930 +مستوى النظافة عندهم غير معروف لجميع تمام؟ من مصادر + +605 +00:45:04,930 --> 00:45:10,150 +مختلفة قد تتلوت المياه هناك فلابد من continuous + +606 +00:45:10,150 --> 00:45:15,170 +change أو disinfection للwater فالكلورين أحد أهم + +607 +00:45:15,170 --> 00:45:20,430 +المركبات كمان شغل مهمة احنا المياه في قطاع غزة + +608 +00:45:20,430 --> 00:45:25,240 +مصدرها الأساسي المياه الجوفيةغالبا المياه الجوفية + +609 +00:45:25,240 --> 00:45:30,640 +لإن الماء بنزل و بتفلتر و بصل لخزان الجوفي، بتفلتر + +610 +00:45:30,640 --> 00:45:34,380 +عبر سبعين، ستين، خمسين متر من الرمل و الطين و + +611 +00:45:34,380 --> 00:45:40,360 +الحصر، فبنزل pure تحت، نقي جدا، لكن لما بنسحب و + +612 +00:45:40,360 --> 00:45:45,060 +احنا و بنوزع في الشبكة، تمام؟ مابنعرفش الشبكة إيش + +613 +00:45:45,060 --> 00:45:52,180 +فيهاطب روحه هم حاطين dosage من ال chlorine بحيث + +614 +00:45:52,180 --> 00:45:56,900 +يصلك انت في المنزل تمام في ما يسمى بال residual + +615 +00:45:56,900 --> 00:46:02,120 +chlorine ال residual يعني كمية المتبقية مسموح + +616 +00:46:02,120 --> 00:46:06,500 +بيها، مسموح بيها يعني غير ضارة للإنسان حتى يقتل أي + +617 +00:46:06,500 --> 00:46:10,080 +ميكروبات قد تعلق في البكتيريا من الشبكة طيب بلاش + +618 +00:46:10,080 --> 00:46:14,050 +احنا هنا ground water نظيفطب تعالى نروح على دولة + +619 +00:46:14,050 --> 00:46:16,970 +أخرى معتمدة على ال surface water و لا افترض نهر + +620 +00:46:16,970 --> 00:46:20,970 +النيل و نهر النيل يمكن شوفته انتوا بعض المناظر هنا + +621 +00:46:20,970 --> 00:46:24,510 +ال youths بتكون طافية لحيوانات في ناس بتغسل على + +622 +00:46:24,510 --> 00:46:29,950 +ضفاف النهر يعني بيحتوي على كام كبير من الملوثات + +623 +00:46:29,950 --> 00:46:35,970 +فكيف لنا أن نقول ان هذا الماء صار للشرب؟ بدنا + +624 +00:46:35,970 --> 00:46:40,150 +ندخله .. بدنا نشيل المواد العالقة فيه و هذه خطوة + +625 +00:46:40,150 --> 00:46:42,550 +بيجون فيها الأخوة البيئيينو بعدين نعمله + +626 +00:46:42,550 --> 00:46:46,130 +disinfection .. و بعدين نعمله disinfection ب .. + +627 +00:46:46,130 --> 00:46:49,850 +برضه ال chlorine .. بسبب ال chlorine أداء مهمة جدا + +628 +00:46:49,850 --> 00:46:54,630 +جدا .. كمان شغلة ال waste water .. المياه العادمة + +629 +00:46:54,630 --> 00:46:59,250 +.. لما أحنا نتخلص منها في البحر .. الأصل قبل أن + +630 +00:46:59,250 --> 00:47:03,650 +نتخلص منها في البحر .. تمام .. ينحط معاها برضه ال + +631 +00:47:03,650 --> 00:47:07,860 +chlorineعشان يقتل الميكروبات لإن احنا .. احنا + +632 +00:47:07,860 --> 00:47:11,480 +بنتخلص من المجاري في البحر و في الصيف بروح نلحق + +633 +00:47:11,480 --> 00:47:20,460 +للمجاري تبعتنا هذه طب عنواني الأمر مابيخلاش من بعض + +634 +00:47:20,460 --> 00:47:23,760 +.. مابيخلاش من بعض ال .. ال يعني خدنا نقول الأشياء + +635 +00:47:23,760 --> 00:47:27,900 +الإشبارية معظم دول العالم بتتخلص مني فهي تبعتها في + +636 +00:47:27,900 --> 00:47:31,220 +الأجسام بتاعة المائية .. الأجسام المائية في البحار + +637 +00:47:32,260 --> 00:47:36,320 +حتى نقلل الضرر، بنقوم بعملته اللي هو الـ + +638 +00:47:36,320 --> 00:47:39,040 +disinfection أو المعالجة، بيسموها المعالجة + +639 +00:47:39,040 --> 00:47:42,680 +الثالثية وبعض الدول الآن أصبحت ال wastewater + +640 +00:47:42,680 --> 00:47:48,540 +بالنسبة لكنزي strategy ممنوع يتضخه في البحر وإنما + +641 +00:47:48,540 --> 00:47:53,000 +يجب أن نستخدم لرأي المزرعة تونس دولة رائدة من + +642 +00:47:53,000 --> 00:47:56,300 +الدول الرائدة اللي بدأت تجربة رائدة فيه إنها تسقي + +643 +00:47:56,300 --> 00:48:01,540 +الحدائق العامة باستخدام المياه المعالجةمعالجة + +644 +00:48:01,540 --> 00:48:07,580 +ثالثية يعني فيها نزال العلق والبكتيرين نكمل المرة + +645 +00:48:07,580 --> 00:48:08,380 +القادمة ان شاء الله + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/Wu7cQmVXlBc_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..8bc4ab639385e8ab70c452687318e469f6798075 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{"start": 1292.39, "end": 1293.71, "word": " الكفاءة", "probability": 0.9119873046875}, {"start": 1293.71, "end": 1294.23, "word": " الصالح", "probability": 0.9025065104166666}, {"start": 1294.23, "end": 1295.03, "word": " للعمل", "probability": 0.849609375}, {"start": 1295.03, "end": 1295.45, "word": " ال", "probability": 0.916015625}, {"start": 1295.45, "end": 1295.85, "word": " major", "probability": 0.85302734375}], "temperature": 1.0}, {"id": 48, "seek": 132043, "start": 1305.11, "end": 1320.43, "text": "مهم جدا لأنه بيجينا كتير مراكبات باستمرار احنا لازم نحدد فعاليتها عن طريق مقارنة بمين بالفينول ويسمى فينول coefficient معامل الفينول", "tokens": [2304, 16095, 10874, 28259, 5296, 33456, 3224, 4724, 1829, 7435, 1829, 8315, 9122, 2655, 13546, 3714, 23557, 4117, 3555, 9307, 4724, 995, 14851, 29973, 9640, 1975, 5016, 8315, 5296, 31377, 2304, 8717, 24401, 3215, 6156, 3615, 6027, 36081, 11296, 18871, 23032, 16572, 4587, 3714, 4587, 9640, 1863, 3660, 4724, 2304, 9957, 20666, 5172, 9957, 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"end": 1312.37, "word": " نحدد", "probability": 0.9373372395833334}, {"start": 1312.37, "end": 1313.33, "word": " فعاليتها", "probability": 0.8525390625}, {"start": 1313.33, "end": 1313.61, "word": " عن", "probability": 0.921875}, {"start": 1313.61, "end": 1313.99, "word": " طريق", "probability": 0.990234375}, {"start": 1313.99, "end": 1314.51, "word": " مقارنة", "probability": 0.89951171875}, {"start": 1314.51, "end": 1314.95, "word": " بمين", "probability": 0.6764322916666666}, {"start": 1314.95, "end": 1316.19, "word": " بالفينول", "probability": 0.7559814453125}, {"start": 1316.19, "end": 1317.91, "word": " ويسمى", "probability": 0.76007080078125}, {"start": 1317.91, "end": 1318.25, "word": " فينول", "probability": 0.7488606770833334}, {"start": 1318.25, "end": 1318.77, "word": " coefficient", "probability": 0.6640625}, {"start": 1318.77, "end": 1319.67, "word": " معامل", "probability": 0.7670084635416666}, {"start": 1319.67, "end": 1320.43, "word": " الفينول", "probability": 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صار ال mutation أو it acquired a gene of resistance البكتيريا صارت resistant لمين؟ لل antibiotic لكن لحد الآن كده استخدامنا ال detol تمام ال resistant لل detol يكاد لا يذكر ال resistant لل chlorine اللي هو ال chlorine يكاد لا يذكر لأنه جالبا هو ال mechanism هي non specific و في نفس الوقت قد تكون ايه؟ Oligodynamic", "tokens": [5172, 1211, 2407, 20328, 9640, 2423, 27960, 6156, 995, 37983, 9957, 2423, 29243, 9673, 5016, 46958, 25720, 21136, 16758, 28671, 22807, 45164, 20328, 9640, 2423, 27960, 34051, 309, 17554, 257, 12186, 295, 7335, 29739, 4117, 2655, 13546, 25528, 20328, 9640, 2655, 20383, 32767, 9957, 22807, 24976, 37828, 44381, 5296, 24401, 6024, 48506, 9122, 3215, 3224, 44713, 9778, 3215, 10943, 8315, 2423, 1141, 401, 46811, 10943, 2423, 20383, 24976, 1141, 401, 7251, 4117, 18513, 20193, 7251, 8848, 37983, 2423, 20383, 24976, 39888, 13672, 1829, 31439, 2423, 39888, 7251, 4117, 18513, 20193, 7251, 8848, 37983, 5296, 33456, 3224, 10874, 6027, 3555, 995, 31439, 2423, 7513, 39896, 2107, 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"probability": 0.76318359375}, {"start": 2198.01, "end": 2198.71, "word": " البكتيريا", "probability": 0.937109375}, {"start": 2198.71, "end": 2199.51, "word": " عندها", "probability": 0.98193359375}, {"start": 2199.51, "end": 2200.13, "word": " hydrophilic", "probability": 0.7613525390625}, {"start": 2200.13, "end": 2200.21, "word": " و", "probability": 0.95458984375}, {"start": 2200.21, "end": 2200.73, "word": " hydrophobic", "probability": 0.91650390625}, {"start": 2200.73, "end": 2201.91, "word": " end؟", "probability": 0.533447265625}, {"start": 2201.91, "end": 2201.99, "word": " ال", "probability": 0.209228515625}, {"start": 2201.99, "end": 2202.15, "word": " cell", "probability": 0.55126953125}, {"start": 2202.15, "end": 2202.55, "word": " membrane", "probability": 0.8701171875}, {"start": 2202.55, "end": 2203.19, "word": " و", "probability": 0.4765625}, {"start": 2203.19, "end": 2203.35, "word": " ال", "probability": 0.884765625}, {"start": 2203.35, "end": 2203.75, "word": " outer", "probability": 0.64111328125}, {"start": 2203.75, "end": 2204.75, "word": " membrane", "probability": 0.93115234375}, {"start": 2204.75, "end": 2205.09, "word": " تبع", "probability": 0.8951822916666666}, {"start": 2205.09, "end": 2205.23, "word": " ال", "probability": 0.76123046875}, {"start": 2205.23, "end": 2205.43, "word": " gram", "probability": 0.63427734375}, {"start": 2205.43, "end": 2205.89, "word": " negative", "probability": 0.65966796875}, {"start": 2205.89, "end": 2206.43, "word": " بكتيريا،", "probability": 0.8324788411458334}, {"start": 2206.43, "end": 2206.71, "word": " مضطر", "probability": 0.69915771484375}, {"start": 2206.71, "end": 2208.19, "word": " لأ؟", "probability": 0.7989908854166666}, {"start": 2208.19, "end": 2208.97, "word": " طيب", "probability": 0.9635416666666666}, {"start": 2208.97, "end": 2209.31, "word": " لما", "probability": 0.800537109375}, {"start": 2209.31, "end": 2209.89, "word": " ييجي", "probability": 0.888916015625}, {"start": 2209.89, "end": 2211.37, "word": " surfactant", "probability": 0.9114583333333334}, {"start": 2211.37, "end": 2212.35, "word": " إله", "probability": 0.5311279296875}, {"start": 2212.35, "end": 2213.13, "word": " hydrophobic", "probability": 0.9049479166666666}, {"start": 2213.13, "end": 2213.57, "word": " end", "probability": 0.9599609375}, {"start": 2213.57, "end": 2214.21, "word": " و", "probability": 0.9765625}, {"start": 2214.21, "end": 2215.11, "word": " يتفاعل", "probability": 0.9283203125}, {"start": 2215.11, "end": 2215.43, "word": " مع", "probability": 0.99169921875}, {"start": 2215.43, "end": 2215.67, "word": " ال", "probability": 0.94775390625}, {"start": 2215.67, "end": 2216.55, "word": " hydrophilic", "probability": 0.9423828125}, {"start": 2216.55, "end": 2216.99, "word": " end", "probability": 0.95654296875}, {"start": 2216.99, "end": 2217.77, "word": " تبع", "probability": 0.9217122395833334}, {"start": 2217.77, "end": 2217.99, "word": " ال", "probability": 0.8583984375}, {"start": 2217.99, "end": 2218.31, "word": " cell", "probability": 0.9619140625}, {"start": 2218.31, "end": 2218.69, "word": " membrane", "probability": 0.9609375}], "temperature": 1.0}, {"id": 82, "seek": 224732, "start": 2219.5, "end": 2247.32, "text": "مين أقوى ال surfactant ولا ال cell membrane فبروح اللي هو شادد ممزق ال cell membrane فاكرين ال cell membrane كيف وصفناها لما وصفنا زيت زيتون يعني لزج جدا مرن جدا رقيق جدا طبعا صلاته ضعيفة جدا وبالتالي من السهل تمزيقه لكن بتلاعب ان ال surfactant بيشتغل", "tokens": [2304, 9957, 5551, 4587, 2407, 7578, 2423, 9684, 578, 394, 49429, 2423, 2815, 19651, 6156, 26890, 2407, 5016, 13672, 1829, 31439, 13412, 18513, 3215, 3714, 2304, 11622, 4587, 2423, 2815, 19651, 6156, 995, 37983, 9957, 2423, 2815, 19651, 9122, 33911, 4032, 9381, 5172, 8315, 11296, 5296, 15042, 4032, 9381, 5172, 8315, 30767, 36081, 30767, 36081, 11536, 37495, 22653, 5296, 11622, 7435, 10874, 28259, 3714, 2288, 1863, 10874, 28259, 12602, 38436, 4587, 10874, 28259, 23032, 3555, 3615, 995, 20328, 15040, 47395, 48812, 3615, 33911, 3660, 10874, 28259, 46599, 6027, 2655, 6027, 1829, 9154, 21136, 3224, 1211, 46811, 11622, 1829, 4587, 3224, 44381, 39894, 15040, 3615, 3555, 16472, 2423, 9684, 578, 394, 4724, 1829, 8592, 2655, 17082, 1211], "avg_logprob": -0.21247328754164216, "compression_ratio": 1.7777777777777777, "no_speech_prob": 0.0, "words": [{"start": 2219.5, "end": 2219.84, "word": "مين", "probability": 0.650390625}, {"start": 2219.84, "end": 2220.36, "word": " أقوى", "probability": 0.8583984375}, {"start": 2220.36, "end": 2220.72, "word": " ال", "probability": 0.65087890625}, {"start": 2220.72, "end": 2221.58, "word": " surfactant", "probability": 0.67510986328125}, {"start": 2221.58, "end": 2222.1, "word": " ولا", "probability": 0.6083984375}, {"start": 2222.1, "end": 2222.3, "word": " ال", "probability": 0.78857421875}, {"start": 2222.3, "end": 2222.46, "word": " cell", "probability": 0.61279296875}, {"start": 2222.46, "end": 2222.82, "word": " membrane", "probability": 0.9228515625}, {"start": 2222.82, "end": 2224.64, "word": " فبروح", "probability": 0.664703369140625}, {"start": 2224.64, "end": 2224.76, "word": " اللي", "probability": 0.75537109375}, {"start": 2224.76, "end": 2224.94, "word": " هو", "probability": 0.9267578125}, {"start": 2224.94, "end": 2225.6, "word": " شادد", "probability": 0.8873697916666666}, {"start": 2225.6, "end": 2227.42, "word": " ممزق", "probability": 0.9066162109375}, {"start": 2227.42, "end": 2228.7, "word": " ال", "probability": 0.459716796875}, {"start": 2228.7, "end": 2228.86, "word": " cell", "probability": 0.85546875}, {"start": 2228.86, "end": 2229.1, "word": " membrane", "probability": 0.89697265625}, {"start": 2229.1, "end": 2229.8, "word": " فاكرين", "probability": 0.9185791015625}, {"start": 2229.8, "end": 2229.92, "word": " ال", "probability": 0.84521484375}, {"start": 2229.92, "end": 2230.06, "word": " cell", "probability": 0.97021484375}, {"start": 2230.06, "end": 2230.32, "word": " membrane", "probability": 0.94189453125}, {"start": 2230.32, "end": 2230.52, "word": " كيف", "probability": 0.897705078125}, {"start": 2230.52, "end": 2231.24, "word": " وصفناها", "probability": 0.9373046875}, {"start": 2231.24, "end": 2231.98, "word": " لما", "probability": 0.729248046875}, {"start": 2231.98, "end": 2232.3, "word": " وصفنا", "probability": 0.992431640625}, {"start": 2232.3, "end": 2232.48, "word": " زيت", "probability": 0.800048828125}, {"start": 2232.48, "end": 2232.96, "word": " زيتون", "probability": 0.794921875}, {"start": 2232.96, "end": 2234.68, "word": " يعني", "probability": 0.7958984375}, {"start": 2234.68, "end": 2235.42, "word": " لزج", "probability": 0.9156901041666666}, {"start": 2235.42, "end": 2236.0, "word": " جدا", "probability": 0.994873046875}, {"start": 2236.0, "end": 2236.64, "word": " مرن", "probability": 0.74951171875}, {"start": 2236.64, "end": 2237.1, "word": " جدا", "probability": 0.983642578125}, {"start": 2237.1, "end": 2237.9, "word": " رقيق", "probability": 0.9832356770833334}, {"start": 2237.9, "end": 2238.44, "word": " جدا", "probability": 0.996826171875}, {"start": 2238.44, "end": 2238.94, "word": " طبعا", "probability": 0.80438232421875}, {"start": 2238.94, "end": 2240.2, "word": " صلاته", "probability": 0.81494140625}, {"start": 2240.2, "end": 2240.74, "word": " ضعيفة", "probability": 0.931884765625}, {"start": 2240.74, "end": 2241.02, "word": " جدا", "probability": 0.993896484375}, {"start": 2241.02, "end": 2241.62, "word": " وبالتالي", "probability": 0.90966796875}, {"start": 2241.62, "end": 2242.08, "word": " من", "probability": 0.9921875}, {"start": 2242.08, "end": 2242.48, "word": " السهل", "probability": 0.98828125}, {"start": 2242.48, "end": 2243.22, "word": " تمزيقه", "probability": 0.96025390625}, {"start": 2243.22, "end": 2243.64, "word": " لكن", "probability": 0.75390625}, {"start": 2243.64, "end": 2245.1, "word": " بتلاعب", "probability": 0.4470672607421875}, {"start": 2245.1, "end": 2245.32, "word": " ان", "probability": 0.3408203125}, {"start": 2245.32, "end": 2245.56, "word": " ال", "probability": 0.85302734375}, {"start": 2245.56, "end": 2246.3, "word": " surfactant", "probability": 0.9513346354166666}, {"start": 2246.3, "end": 2247.32, "word": " بيشتغل", "probability": 0.9791666666666666}], "temperature": 1.0}, {"id": 83, "seek": 226807, "start": 2247.83, "end": 2268.07, "text": "و احنا مش قولنا بدمر الcell membrane عبر مين؟ عبر ال hydrophobic chain و بيرتبط مع الميه في المحيط عبر ال hydrophobic chain فبيقتل البكتيريا و ممكن يزيلها بمعنى اخر زي ما بيشتغل مع الأوساخ، بيرتبط فيها و بيسحبها", "tokens": [2407, 1975, 5016, 8315, 37893, 12174, 12610, 8315, 47525, 29973, 2423, 4164, 19651, 6225, 26890, 3714, 9957, 22807, 6225, 26890, 2423, 5796, 11741, 22234, 5021, 4032, 4724, 13546, 2655, 3555, 9566, 20449, 9673, 1829, 3224, 8978, 9673, 5016, 1829, 9566, 6225, 26890, 2423, 5796, 11741, 22234, 5021, 6156, 21292, 38149, 1211, 29739, 4117, 2655, 13546, 25528, 4032, 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"end": 2259.19, "word": " عبر", "probability": 0.9892578125}, {"start": 2259.19, "end": 2259.29, "word": " ال", "probability": 0.9794921875}, {"start": 2259.29, "end": 2260.05, "word": " hydrophobic", "probability": 0.79150390625}, {"start": 2260.05, "end": 2260.57, "word": " chain", "probability": 0.89599609375}, {"start": 2260.57, "end": 2261.65, "word": " فبيقتل", "probability": 0.9244384765625}, {"start": 2261.65, "end": 2262.25, "word": " البكتيريا", "probability": 0.93935546875}, {"start": 2262.25, "end": 2262.39, "word": " و", "probability": 0.97314453125}, {"start": 2262.39, "end": 2262.57, "word": " ممكن", "probability": 0.7362060546875}, {"start": 2262.57, "end": 2263.13, "word": " يزيلها", "probability": 0.991943359375}, {"start": 2263.13, "end": 2263.63, "word": " بمعنى", "probability": 0.9376953125}, {"start": 2263.63, "end": 2264.01, "word": " اخر", "probability": 0.795166015625}, {"start": 2264.01, "end": 2264.53, "word": " زي", "probability": 0.751708984375}, {"start": 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استخدمت كايش ك skin العملية اللي بيسميها disinfection", "tokens": [27842, 5551, 8592, 3224, 2288, 9673, 31747, 3555, 9307, 9673, 8592, 3224, 13063, 3660, 11331, 26108, 3660, 24525, 2304, 11296, 3964, 1229, 75, 12718, 2197, 761, 6746, 482, 37037, 2407, 7251, 14851, 9778, 40448, 9122, 15154, 4386, 15154, 33334, 394, 34051, 3178, 33334, 394, 37037, 2407, 7251, 2655, 3555, 3615, 30731, 19446, 7251, 38251, 7578, 20666, 2326, 771, 77, 822, 2012, 3317, 2197, 318, 2749, 2326, 771, 77, 822, 2012, 3317, 2197, 6620, 4432, 34051, 2326, 771, 77, 822, 2012, 3317, 2197, 29629, 3964, 1229, 75, 12718, 2197, 44713, 9778, 40448, 2655, 9122, 47302, 8592, 9122, 3178, 2423, 25957, 1211, 10632, 13672, 1829, 4724, 1829, 38251, 1829, 11296, 33334, 313], "avg_logprob": -0.2355011304219564, "compression_ratio": 1.6554621848739495, "no_speech_prob": 0.0, "words": [{"start": 2270.72, "end": 2270.94, "word": "من", "probability": 0.91845703125}, {"start": 2270.94, "end": 2271.28, "word": " أشهر", "probability": 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b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/YSUIUefmWr4.srt @@ -0,0 +1,2495 @@ +1 +00:00:20,570 --> 00:00:25,600 +بسم الله الرحمن الرحيم الموضوع الذي بدرسّه الآن + +2 +00:00:25,600 --> 00:00:29,600 +تفكيرا فيه هو الـ Gram Negative Bacilli هو الـ + +3 +00:00:29,600 --> 00:00:34,460 +Enterobacteriasi ومن الـ Enterobacteriasi لاحظنا + +4 +00:00:34,460 --> 00:00:38,920 +مجموعة اسمها Lactose Fermenting Enterobacteriasi + +5 +00:00:38,920 --> 00:00:41,560 +ومجموعة أخرى Non-Lactose Fermenting وبدأنا بالحديث عن + +6 +00:00:41,560 --> 00:00:44,980 +Lactose Fermenting Enterobacteriasi وأول ما بدأنا + +7 +00:00:44,980 --> 00:00:49,160 +بدأنا عن الإشريشيا كولاي وتكلمنا عنها بمنطقين منطق + +8 +00:00:49,160 --> 00:00:52,260 +as a normal microbiota in the intestinal tract + +9 +00:00:55,950 --> 00:00:59,970 +المنطق التاريخي اللي دخل على الموضوع أنه inside + +10 +00:00:59,970 --> 00:01:03,630 +the intestinal tract some strains were + +11 +00:01:03,630 --> 00:01:07,370 +pathogenic سميناهم entero pathogenic وقالنا قبل + +12 +00:01:07,370 --> 00:01:11,170 +حديث عن entero toxigenic وentero hemorrhagic + +13 +00:01:11,170 --> 00:01:15,610 +وentero invasive ووصلنا آخر شيء لهذا اللوحة انترو + +14 +00:01:15,610 --> 00:01:20,530 +aggregative Escherichia coliكلمة aggregative توحي + +15 +00:01:20,530 --> 00:01:24,630 +بأن البكتيريا لها القدرة على أن تعمل aggregations + +16 +00:01:24,630 --> 00:01:28,170 +يعني groups مع بعض أن لها القدرة على ال attachment + +17 +00:01:28,170 --> 00:01:33,270 +الجيد مع بعضها البعض they cause persistent watery + +18 +00:01:33,270 --> 00:01:37,410 +diarrhea with vomiting and dehydration in infants + +19 +00:01:37,410 --> 00:01:40,850 +يبقى الفئة الأكثر عرضة للإصابة هم ال infants + +20 +00:01:40,850 --> 00:01:45,310 +والأعراض بالأساس هي بتعمل watery diarrhea and + +21 +00:01:45,310 --> 00:01:51,330 +vomiting التي قد تؤدي إلى ال dehydration فاكريني + +22 +00:01:51,330 --> 00:01:53,690 +المحاضرة السابقة أنا ذكرتكم بمعلومة موجودة number + +23 +00:01:53,690 --> 00:01:59,530 +one killer of children in the third world + +24 +00:01:59,530 --> 00:02:04,010 +countries دول العالم الثالث ومعظم الشرق الأوسط من + +25 +00:02:04,010 --> 00:02:08,170 +دول العالم الثالث number one cause هو الدّياريه of + +26 +00:02:08,170 --> 00:02:11,090 +death هو الدّياريه يبقى معناها الحاجة أن الإشريشيا + +27 +00:02:11,090 --> 00:02:13,030 +كولاي ال aggregative أو ال enteroaggregative + +28 +00:02:13,030 --> 00:02:17,270 +invasive صارت تضيف لنا قبعًا جديدًا على هذه الدّياريه + +29 +00:02:17,920 --> 00:02:21,320 +The bacteria adheres to the intestinal mucosa and + +30 +00:02:21,320 --> 00:02:25,140 +elaborates enterotoxins يبقى الميكانيزم by which + +31 +00:02:25,140 --> 00:02:29,120 +they cause infection is by enterotoxins دي + +32 +00:02:29,120 --> 00:02:34,520 +elaborate يعني تبث أو تنتج enterotoxins يَسمّون هذا + +33 +00:02:34,520 --> 00:02:37,740 +الـ enterotoxin اسم خاص يَسمّونه EAST entero + +34 +00:02:37,740 --> 00:02:41,760 +aggregative stable toxin enteroaggregative stable + +35 +00:02:41,760 --> 00:02:45,980 +toxin طبعًا ينتج عنه من ميكوزل دمج أعتقد المحاضرة + +36 +00:02:45,980 --> 00:02:50,910 +السابقة تحدثنا عن أن طالما أن ال mycosa أو ال + +37 +00:02:50,910 --> 00:02:55,810 +mucosal cells أو ال epithelial cells ماتت يبقى + +38 +00:02:55,810 --> 00:02:58,090 +ما فيش absorption جيد وبالتالي بيصير في + +39 +00:02:58,090 --> 00:03:01,790 +accumulation لـ ال electrolytes وعملية الدّياريه + +40 +00:03:01,790 --> 00:03:06,020 +اللي هو الحال أمامنا بتوضّح لنا الـ .. الـ .. الـ + +41 +00:03:06,020 --> 00:03:08,700 +mechanism by which the five types اللي أحنا + +42 +00:03:08,700 --> 00:03:11,060 +ذكرناهم الـ .. الـ .. الـ entero aggregative و الـ + +43 +00:03:11,060 --> 00:03:13,460 +enterotoxigenic و الـ enteroinvasive و الـ + +44 +00:03:13,460 --> 00:03:16,240 +enteropathogenic by .. the mechanism by which they + +45 +00:03:16,240 --> 00:03:19,720 +produce دياري أو infection في ال intestine و + +46 +00:03:19,720 --> 00:03:23,020 +الجدول هذا أيضًا هو نفس المعلومات بس ملخّصة بشكل + +47 +00:03:23,020 --> 00:03:26,540 +مُلخّص في هذا الجدول مش هنمرّ عليهم كمان مرة .. مرة + +48 +00:03:26,540 --> 00:03:29,540 +أخرى لكن أنتم لو طلعتوا عليهم هذا بيفيدكم أنتم + +49 +00:03:29,540 --> 00:03:34,360 +كنوع من التلخيص للمعلومات اللي ذكرناها بالضبط هذه + +50 +00:03:34,360 --> 00:03:37,300 +اللوحة هنجيها في عينها مطوّلًا وإن كان المحتوى + +51 +00:03:37,300 --> 00:03:40,420 +تبعها أربع خمس كلمات لكن هنجيها في عينها مطوّلًا + +52 +00:03:40,420 --> 00:03:43,720 +لأن أحنا بنتكلم عن أو بنختم عن الـ Escherichia coli + +53 +00:03:43,720 --> 00:03:48,580 +ذكرناها كـ normal microbiota ذكرناها كـ outside the + +54 +00:03:48,580 --> 00:03:51,660 +intestinal tract ذكرناها رجعنا كـ intestinal tract + +55 +00:03:51,660 --> 00:03:56,760 +pathogen لأن هنرجعها كـ normal microbiota لما كل + +56 +00:03:56,760 --> 00:04:00,080 +البشر بيحتووا في أمعائهم على الـ Escherichia coli + +57 +00:04:01,010 --> 00:04:05,650 +معنى الحاجة كل ستول بيحتوي على إشريشيا كولاي، كل + +58 +00:04:05,650 --> 00:04:10,250 +سويج في العالم في ستول يبقى فيه إشريشيا كولاي، + +59 +00:04:10,250 --> 00:04:14,550 +number one contaminant أو contamination source for + +60 +00:04:14,550 --> 00:04:18,150 +drinking water all over the world is sewage يعني + +61 +00:04:18,150 --> 00:04:24,410 +ستول يعني إشريشيا كولاي وعليه فقد استخدمت الإشريشيا + +62 +00:04:24,410 --> 00:04:27,210 +كولاي كما يسمّى .. أو كما .. كما يُطلق عليها + +63 +00:04:27,210 --> 00:04:31,230 +indicator water sanitary indicator إيش يعني + +64 +00:04:31,230 --> 00:04:36,570 +sanitary؟ sanitary نظافة صحة نظافة .. نظافة أكثر + +65 +00:04:36,570 --> 00:04:40,390 +من .. من كلمة صحة لما نقول sanitation يعني مستوى + +66 +00:04:40,390 --> 00:04:45,270 +النظافة sanitation conditions تعالوا + +67 +00:04:45,270 --> 00:04:50,890 +نجيب المجلوب جبنا عينة ماء اللي جينا فيها إشريشيا + +68 +00:04:50,890 --> 00:04:58,830 +كولاي من وين بتيجي الإشريشيا كولاي؟ من تلوّث بسوج، + +69 +00:04:58,830 --> 00:05:03,450 +من براز آدمي، يبقى أصبحت الإشريشيا كولاي كـ + +70 +00:05:03,450 --> 00:05:10,670 +indicator على تلوّث الماء أو الغذاء ببراز آدمي طيّب + +71 +00:05:10,670 --> 00:05:14,490 +الإشريشيا كولاي اللي بيحددها لو أحنا شربناها، + +72 +00:05:14,490 --> 00:05:18,090 +تمام؟ هل في هناك ضرر منها إذا كانت من الـ normal + +73 +00:05:18,090 --> 00:05:22,970 +microbiota؟ ما فيش ضرر لكن كمرة أنا ماء أخذتها كـ + +74 +00:05:22,970 --> 00:05:26,090 +pathogen أخذتها كمؤشّر على ورود الـ pathogen بالعربي + +75 +00:05:26,090 --> 00:05:30,010 +البسيط بنقول اللي خلّى الـ Escherichia coli تدخل في + +76 +00:05:30,010 --> 00:05:32,710 +الماء بيخلّي الـ salmonella بيخلّي الـ shigella + +77 +00:05:32,710 --> 00:05:36,350 +بيخلّي الـ yersinia enterocolitica تمام؟ لأن المصدر + +78 +00:05:36,350 --> 00:05:41,430 +تبعهم إيه؟ واحد فمن باب الصحة العامة أنا قريبًا + +79 +00:05:41,430 --> 00:05:46,230 +للأدهار جبنا لواحد منكم كأس من الماء وقلنا لهم + +80 +00:05:46,230 --> 00:05:49,620 +الله أحنا راجينا فيه Escherichia coli الإشيشة كلها + +81 +00:05:49,620 --> 00:05:53,220 +تعني يمكن السلمون اللي موجودة، هيقول له حتى يمكن + +82 +00:05:53,220 --> 00:05:59,400 +لأ، فضل اشرب من هذا الماء تقدر تشرب منّه؟ ليش؟ لأن + +83 +00:05:59,400 --> 00:06:03,500 +احتمالية .. لأن احتمالية وجود الـ salmonella موجودة + +84 +00:06:03,500 --> 00:06:08,700 +فبالتالي أحنا من بداية الصحة العامة الوقاية خير من + +85 +00:06:08,700 --> 00:06:13,700 +علاج وأحنا كمسؤولين عن الصحة العامة نرفض أن الشعب + +86 +00:06:13,700 --> 00:06:18,220 +يأخذ مية فيها E.coli يعني فيها fecal contamination + +87 +00:06:18,220 --> 00:06:23,100 +وبالتالي الـ E.coli كـ water indicator أو كـ food + +88 +00:06:23,100 --> 00:06:28,290 +quality indicator مهم جدًا في هذا الجانب طبعًا هناك + +89 +00:06:28,290 --> 00:06:31,630 +مواصفات ومقاييس يعني مثلًا أحنا بنفحص 100 مللي 100 + +90 +00:06:31,630 --> 00:06:34,810 +مللي من الماء لأنه غالبًا الماء بيكون فيه عدد كبير + +91 +00:06:34,810 --> 00:06:38,190 +من البكتيريا فبنfilter 100 مللي على membrane + +92 +00:06:38,190 --> 00:06:43,310 +filter وبنحطّهم على وسط selective للـ E.coli وبنعدّ + +93 +00:06:43,310 --> 00:06:49,790 +البكتيريا المسموح هو zero مش يعني zero يعني في كل + +94 +00:06:49,790 --> 00:06:54,690 +100 مللي ممنوع يكون فيه ولا E.coli واحدة ولا كل form + +95 +00:06:54,690 --> 00:06:57,410 +واحدة، فيه هنا كان عندي standard قديم بيقول إنه في + +96 +00:06:57,410 --> 00:07:01,530 +الألف لو أنا فلترت ألف ml مش مئة ml، مسموح لحد + +97 +00:07:01,530 --> 00:07:06,410 +ثلاثة على عشر أضعاف الـ .. الـ .. الـ مئة ml، صح؟ + +98 +00:07:06,410 --> 00:07:12,770 +فلو جسمنا ثلاثة على عشرة، بصّي .3، ما فيش حاجة اسمها + +99 +00:07:12,770 --> 00:07:16,510 +.3 بكتيريا، يعني طولت بكتيريا، طولت حبة بكتيريا، + +100 +00:07:16,510 --> 00:07:21,840 +ما فيش، فالرقم بيكون إيه؟ Zero، بيكون Zero ألخّص + +101 +00:07:21,840 --> 00:07:28,160 +الجزء الأخير وجود E.coli في الماء أو في الغذاء + +102 +00:07:28,160 --> 00:07:33,280 +يُعتبر مؤشّر على مدى .. عشان ما .. ما .. ما .. ما + +103 +00:07:33,280 --> 00:07:38,200 +أفتيش فتوى عامة على مدى نظافة هذا الماء وعدم تلوّثه + +104 +00:07:38,200 --> 00:07:42,560 +أو تلوّثه ببراز أخرى طبعًا المصطلح اللي قدامي هنا + +105 +00:07:42,560 --> 00:07:47,980 +مش E.coli، كوليفورم فاكرين اللوحة اللي قرأنا فيها + +106 +00:07:47,980 --> 00:07:51,640 +أسماء الـ Enterobacteriasi؟ كان فيه ثلاثة باللون + +107 +00:07:51,640 --> 00:07:55,160 +البني وكان فيه أربعة تحتيهم خط الأربعة اللي تحتيهم + +108 +00:07:55,160 --> 00:07:57,060 +خط هم هؤلاء الكوليفورم اللي هم الـ lactose + +109 +00:07:57,060 --> 00:08:02,080 +fermenting enterobacterias هم هؤلاء اسمهم كوليفورم + +110 +00:08:02,080 --> 00:08:08,360 +زمان كنا بنستخدم هم الكوليفورم كـ index كمؤشّر للتلوّث + +111 +00:08:08,360 --> 00:08:12,400 +الآن يعني فيه توجه أن نستخدم فقط الإشريشيا كولاي + +112 +00:08:12,400 --> 00:08:16,420 +لأن هي الأكثر تعبيرًا عن التلوّث ببراز آدمي أو + +113 +00:08:16,420 --> 00:08:22,640 +حيواني وصلنا الصورة هذه أرجو ذلك طبعًا ليُدلّ على أهمية + +114 +00:08:22,640 --> 00:08:28,100 +هناك مختبر صحة عامة في قطاع غزة وأكيد فيه في + +115 +00:08:28,100 --> 00:08:32,100 +الضفة الغربية أيضًا هذا المختبر في قسم اسمه water + +116 +00:08:32,100 --> 00:08:35,440 +microbiology تسعين بالمئة من فحوصاته بتدور على + +117 +00:08:35,440 --> 00:08:38,980 +الإشريشيا كولاي أو على الكوليفورم يعني موظّفين شخص فقط + +118 +00:08:38,980 --> 00:08:42,520 +يُعمل العمل يتبع الفلترة ويحطّ الفلترة على الإشريشيا + +119 +00:08:42,520 --> 00:08:48,060 +كولاي وقسم مهم جدًا جدًا جدًا للصحة العامة وعشان أحنا + +120 +00:08:48,060 --> 00:08:51,900 +داخلين على ميكروب ثاني أخذنا نعمل مقدمة أخرى في + +121 +00:08:51,900 --> 00:08:55,440 +الحروب وأحنا عصرنا ثلاث حروب في أقل من ست سنوات + +122 +00:08:55,440 --> 00:09:00,740 +في الحروب الإنسان مقاييسه بتتغير في الغذاء وفي + +123 +00:09:00,740 --> 00:09:03,660 +الشرب يعني في الماء حتى يعني بقى جاي يشرب مية من + +124 +00:09:03,660 --> 00:09:06,220 +فلترة ممكن يشرب مية من البلدية وممكن ينزل لمستوى + +125 +00:09:06,220 --> 00:09:11,120 +أقل من ذلك لو الأمور ساءت وهذا كلام متعارف عليه مش + +126 +00:09:11,120 --> 00:09:14,060 +هنا بس في كل العالم في الحروب مواصفات الإنسان + +127 +00:09:14,060 --> 00:09:20,180 +بتختلف لأن ظروف الحروب أيضا تختلف مش رفاهية طيب هل + +128 +00:09:20,180 --> 00:09:23,940 +الحروب جانب وكل كوعاصرته أنه كان في نقص في المياه + +129 +00:09:23,940 --> 00:09:28,580 +وإنه عملنا إجراءات معينة في بيوتنا كلنا حتى نحافظ + +130 +00:09:28,580 --> 00:09:33,690 +على المياه وكل ما طالت الفترة كل ما زادت قصود اللي + +131 +00:09:33,690 --> 00:09:37,810 +هي الظروف هذه كل ما زادت إجراءاتنا وكل ما قلت + +132 +00:09:37,810 --> 00:09:41,050 +مصادر المياه الشروق .. تعالوا نطلع بضارة بتاعنا + +133 +00:09:41,050 --> 00:09:45,470 +قبل سنتين أو سنة الماضية أظن أننا مطر شديد وفي + +134 +00:09:45,470 --> 00:09:48,770 +مناطق غرقت في غزة غرقت ليش ما اتغرجت يعني .. + +135 +00:09:48,770 --> 00:09:54,270 +يعني أنه ال sewage system نظام المجاري تمام لم + +136 +00:09:54,270 --> 00:09:59,610 +يستوعب كميات الأمطار الغزيرة .. يعني المياه حارة تدخل + +137 +00:10:00,140 --> 00:10:04,180 +الآن احتكاك المياه بالمياه موجود الاتصال بين مياه + +138 +00:10:04,180 --> 00:10:08,040 +المطر ومياه المجاري موجود وبالتالي مياه العادمة + +139 +00:10:08,040 --> 00:10:11,240 +أصبحت موردة في مياه الأمطار اللي بيمشي في الشوارع + +140 +00:10:11,240 --> 00:10:16,340 +اللي انتشرت ووصلت إلى أماكن لم تكن لتصلها المياه + +141 +00:10:16,340 --> 00:10:20,020 +العادمة معناه الحكي أن إمكانية التلوث في هذا + +142 +00:10:20,020 --> 00:10:26,370 +الحالة عالية جدانيجيلا قبل فترة كان فيه tsunami في + +143 +00:10:26,370 --> 00:10:29,470 +إندونيسيا عارفين ال tsunami اللي هي الموجة العالية + +144 +00:10:29,470 --> 00:10:36,310 +من المياه اللي غمرت مناطق كبيرة وقتلت آلاف من + +145 +00:10:36,310 --> 00:10:40,490 +الناس في تلك الفترةعلى طول بعدها بيوم أو يومين + +146 +00:10:40,490 --> 00:10:44,090 +بدأت تظهر تقارير من ال WHO تقول هناك خشية من تفشي + +147 +00:10:44,090 --> 00:10:47,730 +الأوبئة نظرا لعدم توفر مياه الشرب الآمنة، إذا مياه + +148 +00:10:47,730 --> 00:10:53,130 +الشرب الآمنة هي مطلب مش شخصي، مطلب مجتمعي، لذلك + +149 +00:10:53,130 --> 00:10:57,330 +الدولة مسؤولة عن توفير .. الدولة مسؤولة عن توفير + +150 +00:10:57,330 --> 00:11:01,570 +مياه آمنة، عندنا في فلسطين .. في قطاع غزة المصدر + +151 +00:11:01,570 --> 00:11:06,800 +الأساسي لمياه الشرب هو اللي هو المياه الجوفية لكن + +152 +00:11:06,800 --> 00:11:09,460 +المياه الجوفية حتى المياه الجوفية مش مركزية + +153 +00:11:09,460 --> 00:11:13,140 +بمعنى أنه كلها مصدر واحد وكل الناس بتاخد منها من + +154 +00:11:13,140 --> 00:11:17,840 +مصدر واحد، في ناس عندهم آبار خاصة فيهم، في منهم + +155 +00:11:17,840 --> 00:11:21,760 +legal وفي منهم illegal، يعني ماخدين ترخيص وفي ناس + +156 +00:11:21,760 --> 00:11:24,700 +ماخدين مش ماخدين ترخيص، في ناس بيفحصوا المياه + +157 +00:11:24,700 --> 00:11:28,020 +تبعتهم وفي ناس ما فحصوش المياه تبعتهم، يعني الرقابة + +158 +00:11:28,020 --> 00:11:31,420 +على المياه في قطاع غزة في أي في عليه عليه بتهيأت، + +159 +00:11:31,420 --> 00:11:36,490 +استفهام لكن على مستوى الباديات هناك system لمراقبة + +160 +00:11:36,490 --> 00:11:40,470 +المياه اللي تصل للبيوت ولو عملنا مقارنة ما بين + +161 +00:11:40,470 --> 00:11:46,410 +المياه اللي من البلدية والمياه اللي بتشتروها من + +162 +00:11:46,410 --> 00:11:50,630 +البياعين المياه اللي .. المياه المحلقة بتسميها ولا + +163 +00:11:50,630 --> 00:11:54,650 +مفلطقة هتلاقوا أن مستوى النظافة تبعها البلدية أعلى + +164 +00:11:54,650 --> 00:11:58,770 +من ال .. صحيح الملوحة أسوأ .. أه سيئة الملوحة لكن + +165 +00:11:58,770 --> 00:12:07,440 +مستوى النظافة استخدم العملية الكلورة كل ما ذكرته من + +166 +00:12:07,440 --> 00:12:13,240 +المقدمات هو أن تلوث المياه أو مياه الشرب واللي + +167 +00:12:13,240 --> 00:12:18,900 +المؤشر عليه الشريشة كولاي أو الكوليفان خطر كبير + +168 +00:12:18,900 --> 00:12:24,500 +جدا على الصحة العامة ايش المخاطر؟ أحد المخاطر حضرة + +169 +00:12:24,500 --> 00:12:24,920 +مين؟ + +170 +00:12:27,540 --> 00:12:31,560 +شيغيلا فيه أربع أسماء سهلة مش زي ال .. + +171 +00:12:31,560 --> 00:12:33,460 +السالمونيلا .. السالمونيلا فيها أسماء كثيرة الصحيح + +172 +00:12:33,460 --> 00:12:36,820 +..شيغيلا أربع فقط ..شيغيلا فليكسنيري ..شيغيلا + +173 +00:12:36,820 --> 00:12:39,600 +بويدي ..شيغيلا سوناي ..شيغيلا ديزنتيري .. + +174 +00:12:39,600 --> 00:12:44,640 +الأخيرة هي الأهم Dysentery من دوزونطاريا من + +175 +00:12:44,640 --> 00:12:47,480 +dysentery باللغة الإنجليزية و dysentery اللي أنا + +176 +00:12:47,480 --> 00:12:49,560 +وضحت لكم هي أخذوا بالكم المحاضرة اللي ماضية لما + +177 +00:12:49,560 --> 00:12:56,640 +قلت لكم أن الدّياريّا باس ميوكاس زي اللي هو blood هذه + +178 +00:12:56,640 --> 00:13:00,880 +الأربعة اتوفرت في ال characteristics لبراز و هم + +179 +00:13:00,880 --> 00:13:06,300 +نسميها dysentery ال dysentery كانت بتسببها كمان مش + +180 +00:13:06,300 --> 00:13:09,680 +كان، اللي هتسببها بتسببها ال amoeba اللي اسمها + +181 +00:13:09,680 --> 00:13:12,600 +Entamoeba histolytica، درستوها في الثانوية العامة + +182 +00:13:13,460 --> 00:13:15,900 +أو بالثانوية اللي قدرت عليها العامة ولا قبل ذلك + +183 +00:13:15,900 --> 00:13:20,400 +اسمها Entamoeba histolytica وبتعمل dysentery فعشان + +184 +00:13:20,400 --> 00:13:25,380 +نميز ال dysentery تبع الشيجيلا عن ال dysentery تبع + +185 +00:13:25,380 --> 00:13:30,060 +ال .. ال Entamoeba سموها دي Amoeba dysentery و + +186 +00:13:30,060 --> 00:13:33,180 +هيسموها Bacillary Bacillary من شكل البكتيريا + +187 +00:13:33,180 --> 00:13:36,380 +البسلية اللي هو العصرية Bacillary dysentery إذا + +188 +00:13:36,380 --> 00:13:39,260 +المفهوم اللي ريجى اسم Bacillary dysentery عشان + +189 +00:13:39,260 --> 00:13:44,010 +نميزها عن ال Amoeba dysentery بيطلق عليه بعض + +190 +00:13:44,010 --> 00:13:47,470 +الأحيان Shigellosis وده أسهل تسمية Shigella, + +191 +00:13:47,530 --> 00:13:50,350 +Shigellosis, Salmonella, Salmonella's لكن للأسف مش + +192 +00:13:50,350 --> 00:13:55,270 +دايما الأسماء وردية بهذه الطريقة it produces + +193 +00:13:55,270 --> 00:14:00,710 +bloody feces، براز مدمم، intestinal pain، زائد + +194 +00:14:00,710 --> 00:14:05,350 +اللي هو ال pass وكنا تلقائيا فيه mucus من ال + +195 +00:14:05,350 --> 00:14:08,490 +general features إنها بقالها بالعي most strains + +196 +00:14:08,490 --> 00:14:12,270 +cannot ferment lactose وهنا بنكبر شوية المحاضرة + +197 +00:14:17,740 --> 00:14:20,780 +lactose fermenter يعني يا بيض يا أسود يا بتخمر يا + +198 +00:14:20,780 --> 00:14:26,620 +مابتخمرش اليوم بدنا نعرف حالتان slow أو بيسميها + +199 +00:14:26,620 --> 00:14:30,080 +احنا مصطلح قديم اسمها delayed lactose fermenter + +200 +00:14:30,080 --> 00:14:36,480 +delayed lactose fermenter يعني بتخمرش ال lactose + +201 +00:14:36,480 --> 00:14:43,240 +within 24 hours بدها أكثر من 24 hours طب أنا كنت + +202 +00:14:43,240 --> 00:14:47,700 +يعني حازم في المحاضرة قلت lactose fermenter و non + +203 +00:14:47,700 --> 00:14:51,760 +lactose fermenter هل هناك سبب أن أنت راجعت وقلت + +204 +00:14:51,760 --> 00:14:56,940 +أن هم ثلاثة delayed و non lactose و lactose تعالوا + +205 +00:14:56,940 --> 00:15:02,520 +نفهم الموضوع فهم أعمق شوية اللي دخل المختبر ال + +206 +00:15:02,520 --> 00:15:06,790 +microbiology أكيد شاف المكونكي حتى وإن ما عرفش إنه + +207 +00:15:06,790 --> 00:15:11,390 +مكونكي بس أكيد شافه لأن الكليبسيلة اللي نامية على + +208 +00:15:11,390 --> 00:15:16,350 +الطبق اللي لونها أحمر وميكوين تمام أو الاشريكية + +209 +00:15:16,350 --> 00:15:18,930 +كلها اللي لونها أحمر وميكوين هذا المكونكي + +210 +00:15:18,930 --> 00:15:24,990 +المكونكي بنزل عليه البكتيريا لغرض أنه يمنع الإيجرام + +211 +00:15:24,990 --> 00:15:27,770 +بسطه ويسمح للإيجرام نيجاتيف فهو selective for + +212 +00:15:27,770 --> 00:15:32,040 +إيجرام نيجاتيف و differential بيميز بين ال lactose + +213 +00:15:32,040 --> 00:15:34,920 +fermenter و ال non lactose fermenter كيف؟ في + +214 +00:15:34,920 --> 00:15:39,940 +lactose وفي indicator لل pH لما زرعنا البكتيريا + +215 +00:15:39,940 --> 00:15:44,260 +من النمى ل gram نيجاتيف ال gram نيجاتيف بنفترض + +216 +00:15:44,260 --> 00:15:47,660 +الآن يا إما lactose fermenter يا إما non lactose + +217 +00:15:47,660 --> 00:15:52,560 +fermenter إذا lactose fermenter هتعمل fermentation + +218 +00:15:52,560 --> 00:15:59,030 +ل lactose وال result هو acid ال acid هيخفض ال pH و + +219 +00:15:59,030 --> 00:16:02,430 +ال pH indicator هيتغير لونه إلى اللون الأحمر فبيصير + +220 +00:16:02,430 --> 00:16:05,790 +أنك شفت مستعمرة حمراء على المكونكي فهي lactose + +221 +00:16:05,790 --> 00:16:10,730 +fermenter على النقيض إذا ما خمرت ال lactose مافي + +222 +00:16:10,730 --> 00:16:13,550 +acid مافي تغيير ال pH indicator حتى لو المستعمرة + +223 +00:16:13,550 --> 00:16:19,170 +clearness أو ماخدة اللون الوسطي شفافة يعني إذا + +224 +00:16:19,170 --> 00:16:21,410 +lactose fermenter لما lactose fermenter طب وين + +225 +00:16:21,410 --> 00:16:24,470 +الدليل lactose fermenter واحدة lactose ferment .. + +226 +00:16:24,470 --> 00:16:27,590 +non-lactose fermenter لو طلعت الطبق على تلات أيام، + +227 +00:16:27,590 --> 00:16:31,310 +أربع أيام ممكن تتغير لونها وتصل لونها بس أنا + +228 +00:16:31,310 --> 00:16:33,830 +حقيقة لما بقرا الأطباق بقراهاش بعد تلات أيام بقراها + +229 +00:16:33,830 --> 00:16:35,670 +بعد أربع وعشرين ساعة أو أقل حتى من أربع وعشر + +230 +00:16:35,670 --> 00:16:38,770 +ساعة وبالتالي practically أنا بصنف ال .. + +231 +00:16:38,770 --> 00:16:41,790 +practically بصنف البكتيريا إلى lactose و non + +232 +00:16:41,790 --> 00:16:46,440 +-lactose ك practical لكن كواقع they are three + +233 +00:16:46,440 --> 00:16:50,300 +lactose و non lactose وفي النص delayed ال delayed + +234 +00:16:50,300 --> 00:16:53,600 +و ال non lactose بنصنفهم على أنهم non lactose كمان + +235 +00:16:53,600 --> 00:16:56,540 +مرة for practical reasons أن احنا بنقل أطباق بعد + +236 +00:16:56,540 --> 00:17:02,800 +أقل من 24 ساعة وصلت الصورة لكن ممكن نستفيد من + +237 +00:17:02,800 --> 00:17:04,980 +lactose و delayed lactose fermenter و non lactose + +238 +00:17:04,980 --> 00:17:12,280 +fermenter في بعض الفحوصات لما نشتغل ال ABI مثلا كل + +239 +00:17:12,280 --> 00:17:16,500 +ال lactose fermenter وال delayed lactose fermenter + +240 +00:17:16,500 --> 00:17:21,360 +بيكونوا positive لل ONBG ONBG فحص ما أعرفش مش حد + +241 +00:17:21,360 --> 00:17:24,400 +هتندش نقعد نشرح فيه لكن ال non-lactose fermenter + +242 +00:17:24,400 --> 00:17:28,640 +بيكونوا negative فمين ال .. ال .. يعني بمعنى آخر + +243 +00:17:28,640 --> 00:17:31,580 +بقدر أميز بين يعني لو بكتيريا طلعت لي non-lactose + +244 +00:17:31,580 --> 00:17:36,060 +على الطبق وطلعت ONBG positive مش معناها معناها + +245 +00:17:36,060 --> 00:17:41,080 +delayed lactose fermenter لكن لو طلعت حمراء lactose + +246 +00:17:41,080 --> 00:17:44,960 +fermenter برضه ما هتطلعش لأنها ومبجي بسطف، يعني مين + +247 +00:17:44,960 --> 00:17:48,320 +اللي .. مين اللي بيطلع بسطف في ال OMBG؟ ال delayed + +248 +00:17:48,320 --> 00:17:52,340 +و ال lactose fermenter، بينما ال true non-lactose + +249 +00:17:52,340 --> 00:17:57,500 +fermenter بيطلع negative في ال OMBG طيب، هذا + +250 +00:17:57,500 --> 00:18:01,880 +الكلام حكيناه كله عشان خاطر كلمة أنه في شيغيلا + +251 +00:18:01,880 --> 00:18:06,340 +صنعي can ferment lactose slowly وسمينها delayed + +252 +00:18:06,340 --> 00:18:09,710 +lactose fermenting According to O Antigen, they are + +253 +00:18:09,710 --> 00:18:12,230 +classified into four groups، والـ four groups هما + +254 +00:18:12,230 --> 00:18:16,850 +حقيقة كل species منهم group لحالة، كل species طلعت + +255 +00:18:16,850 --> 00:18:20,690 +group لحدها، can easily acquire drug resistance، + +256 +00:18:20,690 --> 00:18:24,630 +زيها زي إيه انتروبكترياسي عند المقدرة إنها تكتسب + +257 +00:18:24,630 --> 00:18:30,500 +مقاومة ال average .. ال average للـ .. للـ .. للـ + +258 +00:18:30,500 --> 00:18:34,060 +incubation period كلمة average مش معناه تحكي أنه + +259 +00:18:34,060 --> 00:18:38,260 +يعني ممكن تكون من واحد لسبع أيام لكن most أو معظم + +260 +00:18:38,260 --> 00:18:41,360 +الناس من اتنين لتلت أيام فما .. هي .. هي أطول من + +261 +00:18:41,360 --> 00:18:46,780 +ذلك يعني لكن هي من واحد إلى سبعة و ممكن معظم الناس + +262 +00:18:46,780 --> 00:18:50,000 +تكون في مرحلة اتنين لتلت أيام و تم ال bacterial + +263 +00:18:50,000 --> 00:18:55,670 +cell damageواشيقلة قبل ما يعني نخد فيه شوية تفاصيل + +264 +00:18:55,670 --> 00:19:01,650 +بدا نذكركوا أنه rarely isolated from sites other + +265 +00:19:01,650 --> 00:19:06,390 +than the intestinal tract إيش يعني؟ نادرا ما عزلت + +266 +00:19:06,390 --> 00:19:09,730 +من أماكن غير الـ intestinal tract يعني بتعمل + +267 +00:19:09,730 --> 00:19:13,310 +localized infection مابتعملش invasive أو systemic + +268 +00:19:13,310 --> 00:19:17,530 +infection هي بتعمل invasion فقط لطبقة رقيقة من الـ + +269 +00:19:17,530 --> 00:19:23,510 +v-line أما مابتقفلش ولا اداركيبقى هي مش systemic + +270 +00:19:23,510 --> 00:19:25,930 +disease تم الـ localize infection في الـ main في الـ + +271 +00:19:25,930 --> 00:19:29,830 +intestinal tract كلكم بتعرفوا أن الـ toxin تبعها + +272 +00:19:29,830 --> 00:19:34,190 +بيعمل destruction للـ .. للـ .. للـ .. للـ .. للـ + +273 +00:19:34,190 --> 00:19:38,370 +ribosome الـ RNA اللي بيشكل الـ ribosome .. بشكل الـ + +274 +00:19:38,370 --> 00:19:41,690 +ribosome بدون ribosome مافي عندنا protein + +275 +00:19:41,690 --> 00:19:45,370 +synthesis واتفقنا protein synthesis بيقضي على .. + +276 +00:19:45,370 --> 00:19:49,290 +إذا توقف تماما بيقضي على الخبر ويوصف الـ toxin بأنه + +277 +00:19:49,290 --> 00:19:53,630 +enterotoxic أو cytotoxic ليش enterotoxic؟ لأنه الـ + +278 +00:19:53,630 --> 00:19:58,170 +effect تبعه في الـ intestine لكن cytotoxic لأنه + +279 +00:19:58,170 --> 00:20:01,310 +برضه يتأثير على كل .. يعني إذا وصل أي خلية بيعملها + +280 +00:20:01,310 --> 00:20:05,390 +destruction فهو generalized effect تمام لكن من حصل + +281 +00:20:05,390 --> 00:20:08,170 +حظنا أنه .. أنه confined في وين؟ في الـ intestinal + +282 +00:20:08,170 --> 00:20:15,030 +tract هذه المعلومة نفس المكررة شوفوا في إمكانية .. + +283 +00:20:15,030 --> 00:20:18,530 +طلعوا أنا على اللوحة دي معلش البكتيريا برضه انت + +284 +00:20:18,530 --> 00:20:21,910 +type 3 secretion system فاكرينه اللي قلنا عنه اللي + +285 +00:20:21,910 --> 00:20:25,190 +بيوحي للبكتيريا بإنه انت phagocyte وإنه + +286 +00:20:25,190 --> 00:20:31,330 +phagocytized the بكتيريا وجوا بيمنع ارتباط الـ + +287 +00:20:31,330 --> 00:20:35,930 +lyosome بالـ phagosome وبتتكاتر البكتيريا واضح أن + +288 +00:20:35,930 --> 00:20:39,690 +البكتيريا عندها المقدرة على الانتقال laterally إيش + +289 +00:20:39,690 --> 00:20:43,330 +يعني laterally؟ يعني بدل ما تدمر الخلية وتروح + +290 +00:20:43,330 --> 00:20:46,710 +وتعمل نفس الـ infection لأ ممكن تنتقل من خلية إلى + +291 +00:20:46,710 --> 00:20:51,930 +خلية مباشرة برضه عبر الـ actin rackets الـ actin + +292 +00:20:51,930 --> 00:20:56,190 +rackets اللي هي قلنا الصواريخ الناتجة عن الـ + +293 +00:20:56,190 --> 00:21:00,570 +polymerization and depolymerization يعني بلمرة و + +294 +00:21:00,570 --> 00:21:03,850 +إعادة تفكيك لـ .. اللي هو الـ .. و أنا قلتلكوا + +295 +00:21:03,850 --> 00:21:07,690 +هحطلكوا صورة أبحث لكوا فيديو بيوضّح حاجة طريقة + +296 +00:21:07,690 --> 00:21:11,750 +رائعة جدا في الـ Listeria Monocytogenes مصورة تحت + +297 +00:21:11,750 --> 00:21:15,130 +الـ microscope صورة حقيقية وطبيعية كيف الـ Listeria + +298 +00:21:15,130 --> 00:21:21,570 +بتنتقل من خلية إلى خلية أخرى، بالأكتينراكيتز الـ + +299 +00:21:21,570 --> 00:21:26,090 +clinical significance لشجلة man is the only + +300 +00:21:26,090 --> 00:21:30,990 +reservoir man أقصد فيها لمين كان مرة الإنسان شفت + +301 +00:21:30,990 --> 00:21:36,710 +الصورة الموجودة على الجانب اللي بيعطي مؤشر سهم من + +302 +00:21:36,710 --> 00:21:42,590 +البراز إلى الفم مشهورة اسمها fecal oral و بالعربي + +303 +00:21:42,590 --> 00:21:47,730 +بدون أي تجميل من البراز إلى الفم هل من البراز إلى + +304 +00:21:47,730 --> 00:21:54,550 +الفم حاجة شائعة أكتر حاجة شائعة في البلد العالم + +305 +00:21:54,550 --> 00:21:58,310 +الثالث لانتقال الأمراض هي من البراز إلى الفم + +306 +00:21:58,310 --> 00:22:03,180 +لماذا؟ أنتو بتنسوا بسرعة لسه مانعش عشرة دقاق نتكلم + +307 +00:22:03,180 --> 00:22:07,580 +على أنه أكبر مصدر ملوث لمياه الشرق في العالم هو الـ + +308 +00:22:07,580 --> 00:22:09,600 +.. هو الـ sewage والـ sewage بيحتاجوا عليها الـ + +309 +00:22:09,600 --> 00:22:12,340 +stool الـ stool اللي هو البراس تمام؟ وبالتالي + +310 +00:22:12,340 --> 00:22:16,840 +إمكانية انتقال الميكروبات و أكبر درجة لذلك .. أكبر + +311 +00:22:16,840 --> 00:22:21,300 +درجة لذلك أن نسبة الأمراض الـ .. الـ .. الـ .. الـ .. + +312 +00:22:21,300 --> 00:22:24,120 +الـ protozoa واللي تنتقل على طريق الفكرة الأولى + +313 +00:22:24,120 --> 00:22:26,960 +العالية عندنا في فلسطين وبالتالي احنا عندنا هذه الـ + +314 +00:22:26,960 --> 00:22:31,170 +.. الـ .. الـ .. الـ .. الـ mood of when بدأت تقل و + +315 +00:22:31,170 --> 00:22:35,710 +بدأت تقل بإيه؟ بتحسن الـ infrastructure تبع الـ + +316 +00:22:35,710 --> 00:22:39,450 +sewage أنه صار فينا closed sewage system نظام + +317 +00:22:39,450 --> 00:22:43,110 +مجاري مغلق وأنا بقولكوا أنه مش كل المناطق مغطاة + +318 +00:22:43,110 --> 00:22:46,690 +يعني مش hundred percent الناس كلها مغطاين لأ في + +319 +00:22:46,690 --> 00:22:51,100 +مناطق لسه ماوصلهاش الـ sewage system وبيستخدموا + +320 +00:22:51,100 --> 00:22:55,280 +القبار اللي هي الشخصية يعني أن كل واحد بيحفرله + +321 +00:22:55,280 --> 00:22:58,860 +حفرة في البيت حفرة إدمصاصية بيسموها septic tank + +322 +00:22:58,860 --> 00:23:03,140 +حفرة إدمصاصية بيتخلص فيها من اللي هو الـ waste + +323 +00:23:03,140 --> 00:23:09,020 +water تبعه أكتر ناس عرضة للإصابة اللي هم الـ young + +324 +00:23:09,020 --> 00:23:13,400 +children فيكل تقرأ الـ contact زي ما حكينا children + +325 +00:23:13,400 --> 00:23:16,040 +to adult طبعا لما يكون الطفل برضه مصاب في البيت + +326 +00:23:16,040 --> 00:23:19,800 +ممكن ينقلها للأخرين النقطة المهمة جدا اللي هي + +327 +00:23:19,800 --> 00:23:22,980 +transmitted by adult food handlers الصورة التانية + +328 +00:23:22,980 --> 00:23:28,820 +على جانب كان ناس كانوا بدخن أظنني وشكلهم يعني مش + +329 +00:23:28,820 --> 00:23:35,520 +انضاف لكن جدهم طعوا وبحضروا الناية انتبهوا ليه + +330 +00:23:35,520 --> 00:23:40,580 +بقى سأقول الآن عملية + +331 +00:23:40,580 --> 00:23:45,080 +تحضير الغداء عملية تحضير الغداء هي بمثابة تحضير + +332 +00:23:45,080 --> 00:23:53,970 +يعني أما هدية طعام صحي أو قنبلة موبوقة لأن .. و + +333 +00:23:53,970 --> 00:23:56,590 +يعتبر على مين الشخص اللي بيحضر و فاكرين جثة + +334 +00:23:56,590 --> 00:23:59,550 +typhoid mallet؟ typhoid mallet لم تغيب على الأذهان + +335 +00:23:59,550 --> 00:24:04,490 +طيب نتخيل أنه في حد في الكفتيريا عندنا في الجامعة + +336 +00:24:04,490 --> 00:24:10,070 +بيشتغل بنظرية typhoid mallet طبعا؟ طيب مين فيكوا + +337 +00:24:10,070 --> 00:24:13,450 +بيروح الكفتيريا؟ ولا حد الحين بطل على البطل يبقى + +338 +00:24:13,450 --> 00:24:17,850 +بيعمل صندوق شاط من البيت طيب طبعا احنا في الجامعة + +339 +00:24:17,850 --> 00:24:22,530 +عندنا رقابة إدارية محترمة جدا على الكفتيريات بتفتش + +340 +00:24:22,530 --> 00:24:27,510 +تفتيش دقيق وهذا اللي بيد إلى التطور اللي حاصل فيه + +341 +00:24:27,510 --> 00:24:35,490 +الكفتيري في قبل 15 سنة عملنا دراسة على مستوى + +342 +00:24:35,490 --> 00:24:40,600 +النظافة المقدمة في الكفتيريا وعملنا يوم دراسي على + +343 +00:24:40,600 --> 00:24:43,680 +هذا الموضوع و .. و .. و دعونا المسؤولين في الجامعة + +344 +00:24:43,680 --> 00:24:47,740 +لحضور .. و بي .. و أبرزنا بعض نقاط الضعف في النظام + +345 +00:24:47,740 --> 00:24:50,800 +و من .. يعني من تقريبا من هذا الطريق إلى اليوم و + +346 +00:24:50,800 --> 00:24:55,020 +هناك بالفعل لجان مشكلة وفيه رقابة وأنا كمان بره + +347 +00:24:55,020 --> 00:25:00,380 +بأبريكو الرقابة يعني وجودها مهم جدا في أي عمل في + +348 +00:25:00,380 --> 00:25:04,240 +الدنيا مافيش رقابة إذا تركنا الأمر للأشخاص غالبا + +349 +00:25:04,240 --> 00:25:07,420 +أشخاص يميلوا إلى التراخي ومن ثم الإهمال زي واحد + +350 +00:25:07,420 --> 00:25:11,600 +بيقعد في المختبروماحدش عليه رقيب يقوله إلبس gloves + +351 +00:25:11,600 --> 00:25:15,000 +أو إلبس المريول أو تعامل بالacid technique بعد + +352 +00:25:15,000 --> 00:25:18,120 +فترة باللجيئة بطل يشتغل هذا الشغل الحالة بقولك يا + +353 +00:25:18,120 --> 00:25:20,900 +عم أنا هنا إلا عشرين سنة بشتغل ولا مقصر بمرض ولا + +354 +00:25:20,900 --> 00:25:25,560 +مقنقل لأحد مرض لكن واقع الأمر عملية المراقبة أمر + +355 +00:25:25,560 --> 00:25:34,200 +ضروري كل ستة أشهر عمال الكفتريات بيخضعوا لفحوصات + +356 +00:25:34,200 --> 00:25:38,460 +بوراز عشان نضمن خلوهم من السمنة والشجرة + +357 +00:25:41,380 --> 00:25:48,760 +تمام؟ هناك تفتيش على الأضافة .. تفتيش على الجروح + +358 +00:25:48,760 --> 00:25:53,060 +.. إذا كان فيه جروح في الإيد .. تمام؟ كمان شغلة .. + +359 +00:25:53,060 --> 00:25:55,520 +بس يمكنها مش بتكون متطبقة .. لكن في المطبخ .. جوا + +360 +00:25:55,520 --> 00:25:57,800 +في المطبخ ممكن يكون متطبقة .. اللي هو الشعر .. و + +361 +00:25:57,800 --> 00:26:01,320 +تقطاته .. يعني من أن يكون الشعر مغطى .. وإذا كان + +362 +00:26:01,320 --> 00:26:04,440 +حتى كمان في لحياة .. يجب أن أيضا كمان تغطى .. لأن + +363 +00:26:04,440 --> 00:26:09,310 +الشعر من الأشياء اللي ممكن تتساقط .. وهي من + +364 +00:26:09,310 --> 00:26:12,370 +المناطق الخارجية في البسمة وبالتالي أكتر عرضة + +365 +00:26:12,370 --> 00:26:16,950 +للتلوث من المناطق الأخرى فبالتالي بيكون هناك نوع + +366 +00:26:16,950 --> 00:26:23,110 +من الرقابة على أي واحد بيعمل في مؤسسة وبيسميها + +367 +00:26:23,110 --> 00:26:26,630 +food handler food handler قد يكون طباخ قد يكون + +368 +00:26:26,630 --> 00:26:31,470 +موزع غذاء زي جرسون أو البياع اللي بيبيع الغذاء + +369 +00:26:31,470 --> 00:26:34,270 +نفسه على الـ counter هذا اللي بيسميهم food handler + +370 +00:26:34,270 --> 00:26:38,430 +طالما هم لمسوا هذا الغذاء إيه دي بقى نفتح لهم رقابة + +371 +00:26:38,430 --> 00:26:43,550 +دوريه ورقابة جيدة حتى نضمن أن .. لأنه نتكلم على + +372 +00:26:43,550 --> 00:26:47,230 +عشرين ألف طالب وطالب في الجامعة أو في الميثال أو + +373 +00:26:47,230 --> 00:26:51,210 +في مؤسسة أو مطعم أو في عزومة أو whatsoever كل هذه + +374 +00:26:51,210 --> 00:26:56,830 +الأشياء ممكن تؤدي إلى انتشار مرض بشكل جماعي إذا لم + +375 +00:26:56,830 --> 00:27:02,370 +نقم نحن بالعمل الصعب unwashed hands + +376 +00:27:06,650 --> 00:27:10,230 +طبعًا البعض بيستهين بهذا الموضوع كثير و بيظن أن + +377 +00:27:10,230 --> 00:27:14,690 +عملية غسل اليدين هي يعني عملية ما أنا أشبه + +378 +00:27:14,690 --> 00:27:19,230 +المسألة في حياتي عشان أنا أغسل يدي باستمرار أنا + +379 +00:27:19,230 --> 00:27:21,630 +بدي أقول لكم كمان مرة مش لأ كمان مرة تغمضوا عينيكم + +380 +00:27:21,630 --> 00:27:25,390 +في البيت و تتخيلوا يديكم هدول تم تم راحوا و + +381 +00:27:25,390 --> 00:27:29,230 +تخيلوا إيش الأشياء اللي أنت كنت تعمليها بالأصابع + +382 +00:27:29,230 --> 00:27:33,830 +لحالهم أصابع هدول الآن مش هتقدر تعمليهم تقريبًا + +383 +00:27:34,680 --> 00:27:39,800 +يمكن بعد خمس دقائق من التفكير هتسود الدنيا و تفقد + +384 +00:27:39,800 --> 00:27:43,200 +الأمل في الحياة و تبطل من الجامعة و تولد في بيته و + +385 +00:27:43,200 --> 00:27:47,320 +بس خمس دقائق من التفكير بأن هدول مش موجودين + +386 +00:27:47,320 --> 00:27:50,760 +حياتنا الآن مربوطة بالـ keyboard و بالـ touch و + +387 +00:27:50,760 --> 00:27:54,480 +بالمقبض و بالفلوس و بنعطي بنمسك الأكل و بنتناول + +388 +00:27:54,480 --> 00:27:58,980 +فيها لو تخيلتوا الأمر اليدين هم الأداة الأكثر + +389 +00:27:58,980 --> 00:28:03,260 +استخدامًا على الإطلاق في أجسامهم هم الأداة الأكثر + +390 +00:28:03,260 --> 00:28:08,320 +استخدامًا في وقت المقابل بيته حتى في الإيماءات لما + +391 +00:28:08,320 --> 00:28:12,320 +نتكلم و نتحرك نتحرك إيدينا و نقول لها واحد اثنين + +392 +00:28:12,320 --> 00:28:16,060 +هناك يعني المعنى الحاكي أن هدول الأداة الأكثر + +393 +00:28:16,060 --> 00:28:20,500 +استخدامًا فهي الأداة الأكثر عرضة للتلوث وبالتالي + +394 +00:28:20,500 --> 00:28:25,680 +عملية غسل اليدين هي عملية مهمة و أذكركم بقص + +395 +00:28:25,680 --> 00:28:30,340 +الإضافة و أذكركم بهيئة وضوء الرسول صلى الله عليه و + +396 +00:28:30,340 --> 00:28:36,630 +سلم كان يكفئ الإناء فيقصل يديه ثم يغرفك، عم يتغسل + +397 +00:28:36,630 --> 00:28:40,470 +إيه؟ هيئة وضوء الرسول صلى الله عليه وسلم بأن كان + +398 +00:28:40,470 --> 00:28:47,210 +يخلل ما بين الأصابع ويدلك عملية تنظيف لو تطلع + +399 +00:28:47,210 --> 00:28:51,090 +على غسل اليدين بالطريقة الحالية هتلاقي أنه كل + +400 +00:28:51,090 --> 00:28:54,170 +الأطباء بيعملوا scrubbing ليدهم وفي مناطق محددة + +401 +00:28:54,170 --> 00:28:59,110 +من اليدين خاصة القريبة من الأظافر بتكون قادرة جدا + +402 +00:28:59,110 --> 00:29:02,190 +لأن هم ده الـ tips اللي احنا بنلمس فيهم تقريبًا كل + +403 +00:29:02,190 --> 00:29:09,150 +شيء هناك مدعاة أن احنا نغسل إيدينا باستمرار + +404 +00:29:09,750 --> 00:29:13,210 +المسألة لو أنا أشتغل في مستشفى أو مختبر و بدي أغسل + +405 +00:29:13,210 --> 00:29:17,510 +يدي باستمرار بعد كل حاجة أعملها معناها حاجة هروح + +406 +00:29:17,510 --> 00:29:21,850 +منهم اليدين هيخلصوا دي من الغسيل مظبوط و مش مظبوط + +407 +00:29:21,850 --> 00:29:25,550 +و بيصيروا الـ dryness اللي بتعرض لفترة طويلة و + +408 +00:29:25,550 --> 00:29:29,410 +بيصيروا cracked و بيصيروا أكثر عرضة يعني الغسيل زاد + +409 +00:29:29,410 --> 00:29:33,650 +من إمكانية أنهم يمرضوا بدل ما يكونوا أصحاء لذلك + +410 +00:29:33,650 --> 00:29:37,790 +يجب أن أحرص على ألا أغسل يدي مرة وتكرار في + +411 +00:29:37,790 --> 00:29:42,210 +المختبر كيف بدي أحصل على هذا الكلام؟ لأ لأ أنا + +412 +00:29:42,210 --> 00:29:45,550 +هتكلم عن الـ gloves كمان شوية ولا الـ gel كمان بدي + +413 +00:29:45,550 --> 00:29:51,210 +أتصرف .. أتصرف .. تمام؟ to minimize contacts .. + +414 +00:29:51,210 --> 00:29:56,410 +تمام؟ contacts مع الـ hands بقى يعني مثلًا الآن قرن + +415 +00:29:56,410 --> 00:30:01,170 +الجوال و أنا قاعد بشتغل بـ gloves و لو بدي أضطر + +416 +00:30:01,170 --> 00:30:03,510 +أشيل الـ gloves كمان و أردع التليفون .. ليش أردع + +417 +00:30:03,510 --> 00:30:08,170 +التليفون؟ ممنوع أردع التليفون في المختبر تمام؟ + +418 +00:30:08,170 --> 00:30:12,070 +ممنوع أمسك قلم و أكتب فيه notes و أنا لابس الـ + +419 +00:30:12,070 --> 00:30:15,550 +gloves لأن بعد شوية هشيل الـ gloves و أغسل إيدي + +420 +00:30:15,550 --> 00:30:18,650 +تمام؟ و أرجع أمسك مين؟ القلم و أحطه في الشنطة و + +421 +00:30:18,650 --> 00:30:21,810 +أروح فيها الضغط و .. و .. و .. و إيه خوتك الصغار + +422 +00:30:21,810 --> 00:30:26,950 +ولا حد تاني من الضغط أخد القلم؟ يعني نجبنا ال .. + +423 +00:30:26,950 --> 00:30:31,390 +ال .. الـ microbe من وين؟ من المستشفى فبدنا نصير + +424 +00:30:31,390 --> 00:30:35,740 +نفكر بطريقة لما بقولكوا في المختبر aseptic + +425 +00:30:35,740 --> 00:30:40,100 +technique ولما بقولكوا في المختبر الإنسان سلوكه + +426 +00:30:40,100 --> 00:30:44,220 +بيختلف عن سلوكه في الشارع بتسمعوا الكلام أولها + +427 +00:30:44,220 --> 00:30:50,180 +بيكون صعب أن أنا أغير سلوكي في مكان معين لكن لما + +428 +00:30:50,180 --> 00:30:55,280 +السلوك أنا أجبره و يصير عادة بيصير بدون تفكير + +429 +00:30:55,280 --> 00:31:00,220 +نفسها بنفسي زي واحد بيفتح الباب ولما يشوف واحد + +430 +00:31:00,220 --> 00:31:07,560 +وراة تمام ينتظر عشان ما يمسكش الباب في وجهه هذه سلوك، + +431 +00:31:07,560 --> 00:31:09,960 +أنا مش مولود في غزل بالمناسبة يعني، لو بلطش في + +432 +00:31:09,960 --> 00:31:15,040 +وجهه عالي ما فيش مشكلة، بس برا سلوك، فتلقائي بتلاقي + +433 +00:31:15,040 --> 00:31:18,540 +الواحد يفتح الباب إذا لمح أو شعر أنه حد وراه بيضل + +434 +00:31:18,540 --> 00:31:23,700 +وجهه لما يتأنى يمسك الباب ويدخل إلى الداخل، بطلت، + +435 +00:31:23,700 --> 00:31:27,520 +بدهاش تفكير، بدهاش جهد، وإنما صارت جزء من مين؟ من + +436 +00:31:27,520 --> 00:31:31,100 +الثقافة، زي ما أنا بالضبط قلت لكم كيف أنا بشتغل + +437 +00:31:31,100 --> 00:31:36,470 +أنبوبتين وبنقل منهم ال .. البكتيريا اللي هم أنبوبتان + +438 +00:31:36,470 --> 00:31:40,230 +بدي أمسك غطا الأولى و غطا الثانية و أمررهم على + +439 +00:31:40,230 --> 00:31:43,870 +اللهب تمام و أمسك واحدة و أحط في الثانية و أرجع + +440 +00:31:43,870 --> 00:31:46,830 +أمررهم على اللهب و أسكر الأولى بالأولى و الثانية + +441 +00:31:46,830 --> 00:31:51,130 +بالثانية طبعًا لو قعدنا نشرحها ربع ساعة و ندربكم + +442 +00:31:51,130 --> 00:31:54,670 +عليها ربع ساعة هتتغلبوا أنا متأكد و هتمارسوا + +443 +00:31:54,670 --> 00:31:58,990 +هتتغلبوا لكن لو مارستوها مرة و مرتين و ثلاثة و + +444 +00:31:58,990 --> 00:32:03,180 +أربعة هتلاقوا أن الأمر صار سهل فأنا بقول استغلوا + +445 +00:32:03,180 --> 00:32:05,920 +فرصة وجودكم في مختبر الـ microbiology لتعديل السلوك + +446 +00:32:05,920 --> 00:32:11,160 +لتعديل السلوك بحيث أنه إذا لبسنا gloves طبعًا الـ + +447 +00:32:11,160 --> 00:32:16,080 +gloves هذه is not to protect yourself only but to + +448 +00:32:16,080 --> 00:32:19,640 +protect others and the environment إذا مسكت أي + +449 +00:32:19,640 --> 00:32:23,360 +أداة حتى لو مش لك في المختبر طبعًا و أنت ماسك الـ + +450 +00:32:23,360 --> 00:32:28,250 +gloves أنت حطيت الميكروبات اللي المفروض تحمل جسمك + +451 +00:32:28,250 --> 00:32:31,990 +يكون محمي منها حطيتها على هذه الأداة صح؟ يعني + +452 +00:32:31,990 --> 00:32:34,410 +بجمعنا الحاكي أنا مش أن أنا لبست الـ gloves يعني + +453 +00:32:34,410 --> 00:32:39,190 +أنا protected و الآخرين protected لأ بالعكس إذا + +454 +00:32:39,190 --> 00:32:44,390 +لبست الـ gloves و تصرفت بسلوك aseptic technique + +455 +00:32:44,390 --> 00:32:47,910 +نعم هيك أنا بكون اشتغلت صح ب .. بس إذا لبست gloves + +456 +00:32:47,910 --> 00:32:51,850 +و أنا بتعطيكم هو بس يعني خالي منطق الموضوع لو + +457 +00:32:51,850 --> 00:32:56,040 +حطينا على عتبة الباب اللي برا هنا مادة مشعة و + +458 +00:32:56,040 --> 00:32:59,840 +جيتوا تفضلوا على الصف ما حدش شافها المادة اللي + +459 +00:32:59,840 --> 00:33:02,780 +شافها ولا حد بيعرفها منكم كلكم خدتوها و خدتوها + +460 +00:33:02,780 --> 00:33:08,480 +على يدكم و دخلتوا الصف طبعًا الآن طفلنا رضوان و + +461 +00:33:08,480 --> 00:33:12,580 +شغلنا إضاءة بالجهاز المعينة بتكشف المادة المشعة + +462 +00:33:15,070 --> 00:33:18,270 +وشفناكم وشفنا وين المادة المشعة حتى لا يجئها + +463 +00:33:18,270 --> 00:33:22,910 +الجرابين تحت، بالشعر هنا، حسب وين الواحد حط إيده + +464 +00:33:22,910 --> 00:33:27,510 +اللي لمسها، صح؟ هتستغرب وين موجودة، هتدور في الشرط + +465 +00:33:27,510 --> 00:33:32,110 +بتاعتكم، في الدفاتر، عن نظارات، في أماكن مختلفة و + +466 +00:33:32,110 --> 00:33:36,750 +متنوعة من أجسامكم، تمام؟ موجودة، المادة المشعة، + +467 +00:33:36,750 --> 00:33:42,170 +و هذا الكلام بيعملوه كاختبار لتوظيف بعض الأحيان في + +468 +00:33:42,170 --> 00:33:43,110 +بعض المؤسسات الكبيرة + +469 +00:33:46,440 --> 00:33:50,560 +بيدخلوا المرشحين للوظيفة عبر الباب الـ hand اللي + +470 +00:33:50,560 --> 00:33:54,020 +تبعتوا فيها المادة المشعة وبعد ساعة بعطوهم تجربة + +471 +00:33:54,020 --> 00:33:59,840 +معينة كويسة اشتغلوها وبعد ساعة أقول لهم stop اطفضوا + +472 +00:33:59,840 --> 00:34:05,080 +ادوضوا شوفوا إن أقلّهم تلوثًا إن هم اللي بياخدوهم و + +473 +00:34:05,080 --> 00:34:08,760 +بيوصلوا أقلّهم تلوثًا بس يكون ده عمل اشتغل مش أقلّهم + +474 +00:34:08,760 --> 00:34:11,320 +تلوثًا يعني أقلّهم تلوثًا إن هم اشتغلوا يكون أعلى + +475 +00:34:11,320 --> 00:34:17,040 +تجربة برضه كمان القصد من الكلام إنه الـ aseptic + +476 +00:34:17,040 --> 00:34:19,660 +technique و الـ hand washing مهم جدا في أي عمل في + +477 +00:34:19,660 --> 00:34:23,480 +البيت في ال .. في ال .. في المطبخ في الجامعة في + +478 +00:34:23,480 --> 00:34:27,520 +المكتبة لكن له أهمية أكبر فيه وخمسة عشر من كل + +479 +00:34:27,520 --> 00:34:31,020 +عام اسمه hand washing day يعني لا تغسلوا إيدكم + +480 +00:34:31,020 --> 00:34:36,140 +إلا في خمسة عشر من كل سنة هو يوم غسيل اليدين + +481 +00:34:36,140 --> 00:34:39,060 +فاستظلوا لهذا اليوم و بصيروا جاجوا إن شاء الله + +482 +00:34:39,060 --> 00:34:45,180 +طبعًا هذا اليوم خصص عشان خاطر تثقيف الناس و العالم + +483 +00:34:45,180 --> 00:34:49,380 +بأهمية غسل اليدين و أنا بقول لكم هي حقيقة علمية و + +484 +00:34:49,380 --> 00:34:52,480 +ليست تسويقية 80% من الـ infectious diseases could be + +485 +00:34:52,480 --> 00:34:56,740 +prevented just by hand washing 80% of infectious + +486 +00:34:56,740 --> 00:35:00,000 +diseases could be prevented just by hand .. مش + +487 +00:35:00,000 --> 00:35:02,420 +عفوًا by hand washing .. by proper hand washing + +488 +00:35:02,930 --> 00:35:05,310 +Proper hand washing اللي هو غسل اليدين بطريقة + +489 +00:35:05,310 --> 00:35:11,690 +الصحيحة طيب الـ infective dose بتاعة البكتيريا + +490 +00:35:11,690 --> 00:35:14,630 +بتتراوح من عشرة إلى مليون حتى وجدت في كتب من واحد + +491 +00:35:14,630 --> 00:35:18,530 +لمليون من واحد لخمسة يعني مختلفة لكن في الآخر هي عدد + +492 +00:35:18,530 --> 00:35:24,130 +قليل والـ ID50 إذا فاكرين هتقول لنا هي مقياس لل + +493 +00:35:24,130 --> 00:35:28,170 +virulence مثلًا بالـ term fresh الـ virulence الـ + +494 +00:35:28,170 --> 00:35:31,930 +virulence كل ما قل الـ ID50 كل ما زاد الـ virulence + +495 +00:35:32,930 --> 00:35:38,530 +قررنا الشغلة بالـ Vibrio واحد أو عشرين لمليون و + +496 +00:35:38,530 --> 00:35:44,410 +بالمقارنة مع مليون خلية يبقى العشرة أو العشرين + +497 +00:35:44,410 --> 00:35:50,640 +لمليون more virulent من اللي هو المليون There is an + +498 +00:35:50,640 --> 00:35:52,700 +incubation period of one to seven days فكده قبل + +499 +00:35:52,700 --> 00:35:54,340 +شوية هتقول لنا أن الـ incubation period من اثنين لثلاثة + +500 +00:35:54,340 --> 00:35:56,860 +أيام هذه الـ average أو الـ most common لكن في شكل + +501 +00:35:56,860 --> 00:36:00,740 +عام ممكن تستمر مع بعض الناس على سبع أيام by Fallot + +502 +00:36:00,740 --> 00:36:04,580 +fever, cramping, مغص, abdominal pain, ألم في + +503 +00:36:04,580 --> 00:36:09,600 +المعدة, water diarrhea due to the toxin هذا + +504 +00:36:09,600 --> 00:36:12,680 +العملية بتستمر من واحد لتلت أيام اللي هو الإسهال + +505 +00:36:12,680 --> 00:36:17,530 +الشريد هذا بعد تلت أيام غالبا الـ .. الـ .. الـ .. الـ + +506 +00:36:17,530 --> 00:36:17,710 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +507 +00:36:17,710 --> 00:36:17,870 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +508 +00:36:17,870 --> 00:36:18,270 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +509 +00:36:18,270 --> 00:36:18,730 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +510 +00:36:18,730 --> 00:36:20,730 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +511 +00:36:20,730 --> 00:36:27,890 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +512 +00:36:27,890 --> 00:36:29,030 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +513 +00:36:29,030 --> 00:36:29,870 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +514 +00:36:29,870 --> 00:36:30,050 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +515 +00:36:30,050 --> 00:36:32,950 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +516 +00:36:32,950 --> 00:36:36,330 +.. الـ + +517 +00:36:40,900 --> 00:36:43,720 +it is rare for the organism to disseminate هذا + +518 +00:36:43,720 --> 00:36:47,600 +الجبل حاجناها، الميكروب غالبا لا يُعزل إلا من وين + +519 +00:36:47,600 --> 00:36:51,460 +من الـintestinal tract the severity of the disease + +520 +00:36:51,460 --> 00:36:54,940 +is dependent on the strain يعني بعتمد على أفعا on + +521 +00:36:54,940 --> 00:36:59,560 +the species أكثرهم شراسة وأكثرهم قدرة على إحداث + +522 +00:36:59,560 --> 00:37:03,600 +severity في الشجلة اللي هو Shigella Dysentery is + +523 +00:37:03,600 --> 00:37:06,840 +the most followed by Flexnery, Sunni and Boy + +524 +00:37:09,440 --> 00:37:16,240 +الـimmunity عشان SIGA هذا soluble immunoglobulin A + +525 +00:37:16,240 --> 00:37:20,700 +أو سيكراتوري اللي هنقول عنه هو بيكون وامو .. + +526 +00:37:20,700 --> 00:37:23,180 +السيكراتوري هذا بيكون على الـmucus membrane على الـ + +527 +00:37:23,180 --> 00:37:28,510 +mucus membrane لكن برضه الـimmunity مش هال .. يعني + +528 +00:37:28,510 --> 00:37:31,830 +لو الإنسان قصيب اليوم شيجيلوسس اللي من الاتباع .. + +529 +00:37:31,830 --> 00:37:35,470 +اللي من الاتباعته may not last for six months حتى + +530 +00:37:35,470 --> 00:37:39,990 +ست شهورها بتكملش، لو .. لو شرب مرة تانية شجيلة + +531 +00:37:39,990 --> 00:37:45,210 +ممكن تعمله infection الـdiagnosis للـ .. للشجيلة + +532 +00:37:45,210 --> 00:37:49,750 +infection غالبا بالستول، stool culture، يعني بناخد + +533 +00:37:49,750 --> 00:37:53,740 +الديارية إذا كان المريض مصل لمرحلة إنه مافيش stool + +534 +00:37:53,740 --> 00:37:57,000 +بنستخدم ما يسمى بالـrectal swab يبقى عندي يا إما + +535 +00:37:57,000 --> 00:38:00,040 +stool و swab يعني swab بناخده في الـrectum و + +536 +00:38:00,040 --> 00:38:02,800 +بنعمله rolling بناخد الـwhatsoever المتيرة + +537 +00:38:02,800 --> 00:38:05,760 +الموجودة أكيد هتكون محتوى على مين؟ على الـmicro + +538 +00:38:05,760 --> 00:38:09,640 +organism بس دايما الـstool عينة مفضلة عن اللي هو + +539 +00:38:09,640 --> 00:38:12,700 +الـrectal swab بس في حالة عدم وجود stool بناخد + +540 +00:38:12,700 --> 00:38:17,160 +اللي هو rectal swab طبعا العملية بتتم في .. في .. + +541 +00:38:17,160 --> 00:38:20,940 +من خلال مزرعة وعملت identification ممكن استخدم + +542 +00:38:20,940 --> 00:38:23,000 +quick immunological method إيش يعني quick + +543 +00:38:23,000 --> 00:38:26,300 +immunological method؟ الستول أنا قولتلكوا دايما في + +544 +00:38:26,300 --> 00:38:30,920 +الأوبئة بنلجأ إلى إنه نقصر فترة الـdiagnosis قدر + +545 +00:38:30,920 --> 00:38:34,580 +المستطاع، مظبوط و مش مظبوط، بمعنى لو احنا في الـ .. + +546 +00:38:34,580 --> 00:38:37,120 +الـ .. الـ .. المزرعة تاخد معنا 72 ساعة، بدنا نحاول + +547 +00:38:37,120 --> 00:38:40,140 +قدر الإمكان خلال 24 ساعة، وإذا أقل خلال نص ساعة، + +548 +00:38:40,140 --> 00:38:44,900 +لماذا .. لماذا .. لماذا أتأخر أكتر من نص ساعة؟ فلـ + +549 +00:38:44,900 --> 00:38:47,960 +أخدنا عينة ستول و عملنا لها fixation على شريحة و + +550 +00:38:47,960 --> 00:38:50,560 +استخدمنا fluorescent antibody إيش يعني fluorescent + +551 +00:38:50,560 --> 00:38:54,860 +antibody؟ يعني antibody ضد الشجلة بس labelled + +552 +00:38:54,860 --> 00:38:59,300 +بـfluorescent و أعطينا فرصة للـantibody اللي هو الـ + +553 +00:38:59,300 --> 00:39:01,700 +fluorescent labelled إنه يتفاعل مع البكتيريا اللي + +554 +00:39:01,700 --> 00:39:05,620 +موجودة بالبراز مليان بكتيريا من الشجلة و غير الشجلة + +555 +00:39:05,620 --> 00:39:11,140 +إذا الشجلة موجودة هيرتبط فيها الـantibody بنغسل + +556 +00:39:11,140 --> 00:39:16,420 +الشريحة بنغسل الشريحة خدوا بالكم البكتيريا fixed + +557 +00:39:16,420 --> 00:39:21,520 +صح؟ على الشريحة الآن لو الـantibody اللي احنا + +558 +00:39:21,520 --> 00:39:25,760 +حطناه هيرتبط بالبكتيريا هيصيب برضه fixed مع + +559 +00:39:25,760 --> 00:39:29,740 +البكتيريا اللي هي fixed على الشريحة فلـو غسلنا مش + +560 +00:39:29,740 --> 00:39:35,200 +هيروح من البكتيريا لكن لو الشيقلة مش موجودة الـ + +561 +00:39:35,200 --> 00:39:39,120 +antibody بيرتبط مع مين؟ ولا شيء فلما نعمل غسيل + +562 +00:39:39,120 --> 00:39:43,420 +بيروح مع الغسيل بعد ما بنجفف الشريحة بنحطها تحت الـ + +563 +00:39:43,420 --> 00:39:47,240 +fluorescent microscope UV بتطلب .. ت .. تضرب + +564 +00:39:47,240 --> 00:39:51,060 +الشريحة إذا في fluorescent .. مادة fluorescent + +565 +00:39:51,060 --> 00:39:55,300 +تمتص الـUV و بتشير لنا visible light إذا أنا شفت + +566 +00:39:55,300 --> 00:39:59,040 +الآن إيش؟ visible light اللون المعينة حسب اللي هو + +567 +00:39:59,040 --> 00:40:02,940 +اللون اللي أنا بستخدمه في مين في الـantibody .. الـ + +568 +00:40:02,940 --> 00:40:06,120 +fluorescent antibody إذا شوفت light معناه إن حاجة الـ + +569 +00:40:06,120 --> 00:40:08,760 +fluorescent موجود ليش الـfluorescent موجود؟ لأن الـ + +570 +00:40:08,760 --> 00:40:12,320 +antibody موجود ليش الـantibody موجود لحد الآن بدل + +571 +00:40:12,320 --> 00:40:15,860 +ما غسيل؟ لأن البكتيريا موجودة خلال نصف ساعة نقدر نعرف + +572 +00:40:15,860 --> 00:40:19,480 +النتيجة إنه positive ولا negative لكن لا تبني عن + +573 +00:40:19,480 --> 00:40:25,300 +الـculture، ليش؟ لأنه بتعمل كمان sensitivity بتشوف + +574 +00:40:25,300 --> 00:40:29,920 +مدى حساسية البكتيريا لمين؟ لـ .. اللي هو الـ .. الـ + +575 +00:40:29,920 --> 00:40:31,760 +.. الـ .. الـ .. الـ .. الـ antibiotics اللي هنقوله + +576 +00:40:31,760 --> 00:40:34,830 +قبل شوية في اللوحات السادقة إن البكتيريا مشهورة + +577 +00:40:34,830 --> 00:40:40,750 +عنها إنها بتكون resistance Prevention فيه vaccine + +578 +00:40:40,750 --> 00:40:44,870 +بستخدمه في المصدر الموضوع Life attenuated shigella + +579 +00:40:44,870 --> 00:40:48,690 +dysenteria vaccine إيش يعني life attenuated يعني + +580 +00:40:48,690 --> 00:40:57,610 +البكتيريا حية بنعملها بمعاملة معينة بنقلل من الـ + +581 +00:40:57,610 --> 00:41:01,320 +violence تبعها بنقلل من الـviolence تبعها فبتعمل + +582 +00:41:01,320 --> 00:41:07,300 +mild infection إينفكشن بسيطة الجهاز المناعي بتغلب + +583 +00:41:07,300 --> 00:41:11,220 +عليها لكن كمان مرة الـimmunity تبعتها short + +584 +00:41:11,220 --> 00:41:16,860 +فبالتالي بتعطى للناس مثلا الدكتور بدروح على يتطوع + +585 +00:41:16,860 --> 00:41:21,520 +في منطقة فيها و بقلة الشجرة قبل ما يروح بيعطوب هذا + +586 +00:41:21,520 --> 00:41:24,540 +الـvaccine عشان هو يحمي نفسه خلال فترة وجوده في + +587 +00:41:24,540 --> 00:41:29,750 +هذه المنطقة كمان مرة بأكدلكوا على أهمية الـSafe + +588 +00:41:29,750 --> 00:41:39,070 +Water في الحروب، في الكوارث، في الأمطار، في + +589 +00:41:39,070 --> 00:41:43,630 +الزلازل، في الهجرات اللي بتصير، يجب أن يقتنع .. + +590 +00:41:43,630 --> 00:41:47,290 +لذلك أنا بقول إن من ثقافة الأفراد على مستوى العالم + +591 +00:41:47,290 --> 00:41:51,250 +يجب أن يكون كيف يتحصل على Safe Water في معظم + +592 +00:41:51,250 --> 00:41:56,490 +الأحوال يعني .. يعني قدر المية استطيع لو بديه + +593 +00:41:56,490 --> 00:42:01,190 +يستخدم جميص ويفلتر الميه يعني أقل .. يعني .. يعني + +594 +00:42:01,190 --> 00:42:05,190 +أحسن من إنه ميفلترهاش لو عنده إمكانية تسخينها أو + +595 +00:42:05,190 --> 00:42:08,950 +غليها بكون أفضل لو عنده إمكانية إضافة بعض قطرات + +596 +00:42:08,950 --> 00:42:13,010 +من الـchlorine على الميه برضه بكون كويس تعرف + +597 +00:42:13,010 --> 00:42:19,310 +الشاب؟ الألم .. الشبه هذه .. تمام؟ هذه بيستخدموها + +598 +00:42:19,310 --> 00:42:23,450 +عالميا في عملية تنقية .. تنقية المياه بإنه ت .. ت + +599 +00:42:23,450 --> 00:42:27,590 +.. ت .. تنطحن بشكل بودرة و لو كانت المياه يعني + +600 +00:42:27,590 --> 00:42:32,830 +مليئة بالشوائب و حاطين عليها الشبه بعد فترة + +601 +00:42:32,830 --> 00:42:37,910 +بترسبها تمام؟ بترسب و بناخد احنا السائل النظيف و + +602 +00:42:37,910 --> 00:42:43,600 +بنفلتره كمرة عبر .. عبر قطعة قماش لو كانت الشباب + +603 +00:42:43,600 --> 00:42:47,620 +المتوفرة وكان الـchlorine متوفر حلت مشكلة الميه، مهما + +604 +00:42:47,620 --> 00:42:51,120 +كانت نوع الميه، تمام؟ غالبا بيقدر إنه نحصل على + +605 +00:42:51,120 --> 00:42:56,620 +مياه آمنة، لكن الغلي دايما بيكون برضه وسيلة جيدة + +606 +00:42:56,620 --> 00:42:59,820 +Treating scoliosis, manage dehydration, manage + +607 +00:42:59,820 --> 00:43:02,940 +electrolytes, patients respond to antibiotics، فيه + +608 +00:43:02,940 --> 00:43:06,900 +إنها مشكلة بكلمات صغيرة problem of drug resistance + +609 +00:43:07,800 --> 00:43:11,380 +أذكركوا بالديارية بشكل عام لتريتمنت تبعتها كانت + +610 +00:43:11,380 --> 00:43:17,820 +تتلخص في تلت شغلات رئيسية water replacement، + +611 +00:43:17,820 --> 00:43:20,440 +electrolyte replacement، هدول التانية اللي حطيناهم + +612 +00:43:20,440 --> 00:43:23,660 +في معلوق محلول معالجة الجفاف، التانية هم بيعطيه + +613 +00:43:23,660 --> 00:43:25,260 +ميه و بيعطيه محلول في نفس الـelectrolyte في نفس + +614 +00:43:25,260 --> 00:43:30,820 +الوقت، تمام؟ التالتة اللي هو الـeradication of the + +615 +00:43:30,820 --> 00:43:33,430 +causative agent ماذا يعني eradication of the cause + +616 +00:43:33,430 --> 00:43:35,910 +of the pain؟ إذا العامل المسبب أنت إيه في + +617 +00:43:35,910 --> 00:43:39,990 +هالستولاتكا، بتدعي المريض antiprotozoa زي + +618 +00:43:39,990 --> 00:43:44,630 +الفلاجيل، زي الفلاجيل إذا كان سالمونيلا أو شجلة، + +619 +00:43:44,630 --> 00:43:48,670 +بتدعي أنت بيتك مناسب لقتل السالمونيلا أو الشجلة، + +620 +00:43:48,670 --> 00:43:52,690 +هذا معناه eradication، إذن هي تلت نقاط رئيسية + +621 +00:43:52,690 --> 00:43:56,290 +water، electrolyte replacement، بعدها eradication + +622 +00:43:56,290 --> 00:44:02,900 +تعتمد على مين المسبب؟ مين المسبب؟ المرة الجاية إن + +623 +00:44:02,900 --> 00:44:06,700 +شاء الله بننتقل لموضوع آخر ليا سلمونيلا و بننهي إن + +624 +00:44:06,700 --> 00:44:07,220 +شاء الله هذا diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/YSUIUefmWr4_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/YSUIUefmWr4_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..70b9c449750bc60b263b54d1c8177c8d13cebc74 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/YSUIUefmWr4_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 2323, "start": 20.57, "end": 23.23, "text": "بسم الله الرحمن الرحيم الموضوع", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 9673, 2407, 11242, 45367], "avg_logprob": -0.20242745961461747, "compression_ratio": 1.2444444444444445, "no_speech_prob": 0.0, "words": [{"start": 20.57, "end": 20.79, "word": "بسم", "probability": 0.409912109375}, {"start": 20.79, "end": 20.93, "word": " الله", "probability": 0.97021484375}, {"start": 20.93, "end": 21.27, "word": " الرحمن", "probability": 0.9700520833333334}, {"start": 21.27, "end": 21.79, "word": " الرحيم", "probability": 0.99462890625}, {"start": 21.79, "end": 23.23, "word": " الموضوع", "probability": 0.954345703125}], "temperature": 1.0}, {"id": 2, "seek": 5226, "start": 24.7, "end": 52.26, "text": "اللي بدرسوا الآن 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{"id": 38, "seek": 95330, "start": 930.76, "end": 953.3, "text": "بيميز بين ال lactose fermenter و ال non lactose fermenter كيف؟ في lactose و في indicator لل pH لما زرعنا البكتيريا من النمى ل gram نيجاتيف ال gram نيجاتيف بنفترض الآن يا إما lactose fermenter يا إما non lactose fermenter إذا lactose fermenter هتعمل fermentation ل lactose", "tokens": [3555, 32640, 1829, 11622, 49374, 2423, 34042, 541, 38300, 260, 4032, 2423, 2107, 34042, 541, 38300, 260, 9122, 33911, 22807, 8978, 34042, 541, 4032, 8978, 16961, 24976, 21677, 5296, 15042, 30767, 2288, 3615, 8315, 29739, 4117, 2655, 13546, 25528, 9154, 28239, 2304, 7578, 5296, 21353, 8717, 1829, 7435, 9307, 1829, 5172, 2423, 21353, 8717, 1829, 7435, 9307, 1829, 5172, 44945, 5172, 2655, 43042, 6024, 48506, 35186, 11933, 15042, 34042, 541, 38300, 260, 35186, 11933, 15042, 2107, 34042, 541, 38300, 260, 11933, 15730, 34042, 541, 38300, 260, 8032, 2655, 25957, 1211, 43161, 5296, 34042, 541], "avg_logprob": -0.26036184147784586, 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982.51, "text": "و ال result هو acid ال acid هيخفض ال pH و ال pH indicator هيتغير لونه إلى اللون الأحمر فبصير انك شفت مستعمرة حمرة على المكونكي فهي lactose fermenter على النقيد اذا ما خمرت ال lactose مافي acid مافي تغيير ال pH indicator حتى لو المستعمرة clearness او ماخدة اللون الوسطى شفافة يعني اذا lactose fermenter لما lactose fermenter طب وين الدليل lactose fermenter", "tokens": [2407, 2423, 1874, 31439, 8258, 2423, 8258, 39896, 9778, 5172, 11242, 2423, 21677, 4032, 2423, 21677, 16961, 39896, 2655, 17082, 13546, 5296, 11536, 3224, 30731, 13672, 11536, 16247, 5016, 29973, 6156, 3555, 9381, 13546, 16472, 4117, 13412, 5172, 2655, 3714, 14851, 25957, 25720, 11331, 2304, 25720, 15844, 9673, 4117, 11536, 4117, 1829, 6156, 3224, 1829, 34042, 541, 38300, 260, 15844, 28239, 4587, 25708, 1975, 15730, 19446, 16490, 29973, 2655, 2423, 34042, 541, 19446, 41185, 8258, 19446, 41185, 6055, 17082, 1829, 13546, 2423, 21677, 16961, 11331, 49975, 45164, 9673, 14851, 25957, 25720, 1233, 16937, 1975, 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b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/YSUIUefmWr4_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..fc86f9a5a3eb682feda73f1edeeea17d5a577849 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/YSUIUefmWr4_raw.srt @@ -0,0 +1,2504 @@ +1 +00:00:20,570 --> 00:00:25,600 +بسم الله الرحمن الرحيم الموضوعاللي بدرسوا الآن + +2 +00:00:25,600 --> 00:00:29,600 +تفكيرا بيه هو الـ Gram Negative Bacilli هو الـ + +3 +00:00:29,600 --> 00:00:34,460 +Enterobacteriasi ومن الـ Enterobacteriasi لاحظنا + +4 +00:00:34,460 --> 00:00:38,920 +مجموعة اسمها Lactose Fermenting Enterobacteriasi + +5 +00:00:38,920 --> 00:00:41,560 +ومجموعة أخرى Lactose Fermenting وبدأنا بالحديث عن + +6 +00:00:41,560 --> 00:00:44,980 +Lactose Fermenting Enterobacteriasi وأول ما بدأنا + +7 +00:00:44,980 --> 00:00:49,160 +بدأنا عن الإشريشيا كولاي وتكلمنا عنها بمنطقين منطق + +8 +00:00:49,160 --> 00:00:52,260 +in a normal microbiota in the intestinal tract + +9 +00:00:55,950 --> 00:00:59,970 +المنطق التاريخي اللي دخل على الموضوع انه inside + +10 +00:00:59,970 --> 00:01:03,630 +the intestinal tract some strands were ورش + +11 +00:01:03,630 --> 00:01:07,370 +pathogenic سميناهم entero pathogenic وقالنا قبل + +12 +00:01:07,370 --> 00:01:11,170 +حديث عن entero toxigenic وentero hemorrhagic + +13 +00:01:11,170 --> 00:01:15,610 +وentero invasive ووصلنا اخر اشي لهذا اللوحة انترو + +14 +00:01:15,610 --> 00:01:20,530 +aggregative Escherichia coliكلمة aggregative توحي + +15 +00:01:20,530 --> 00:01:24,630 +بإن البكتيريا لها المقدرة علي ان تعمل aggregations + +16 +00:01:24,630 --> 00:01:28,170 +يعني groups مع بعض انها المقدرة على ال attachment + +17 +00:01:28,170 --> 00:01:33,270 +الجيد مع بعضها لبعض they cause persistent watery + +18 +00:01:33,270 --> 00:01:37,410 +diarrhea with vomiting and dehydration in infants + +19 +00:01:37,410 --> 00:01:40,850 +يبقى الفئة الأكتر عرضة للإصابة هم ال infants + +20 +00:01:40,850 --> 00:01:45,310 +والأعراض بالأساس هي بتعمل watery diarrhea and + +21 +00:01:45,310 --> 00:01:51,330 +vomitingاللي قد تؤدّجنا إلى ال dehydration فاكريني + +22 +00:01:51,330 --> 00:01:53,690 +المحاضرة السابقة أنا ذكرتكوا بمعلومة موجودة number + +23 +00:01:53,690 --> 00:01:59,530 +one killer of children in the third world + +24 +00:01:59,530 --> 00:02:04,010 +countries دول العالم الثالث ومعظم الشرق الأوسط من + +25 +00:02:04,010 --> 00:02:08,170 +دول العالم الثالث number one cause هو الدياريه of + +26 +00:02:08,170 --> 00:02:11,090 +death هو الدياريه يبجى معناها الحاجة إن الشريشة + +27 +00:02:11,090 --> 00:02:13,030 +كولاعي ال interrogative أو ال interrogative + +28 +00:02:13,030 --> 00:02:17,270 +invasive صارت اضيف لنا قبع جديد على هذه الدياريه + +29 +00:02:17,920 --> 00:02:21,320 +The bacteria adheres to the intestinal mucosa and + +30 +00:02:21,320 --> 00:02:25,140 +elaborates enterotoxins يبقى الميكانيزم by which + +31 +00:02:25,140 --> 00:02:29,120 +they cause infection is by enterotoxins دي + +32 +00:02:29,120 --> 00:02:34,520 +elaborate يعني تبث أو تنتج enterotoxins بيسموا هذا + +33 +00:02:34,520 --> 00:02:37,740 +ال enterotoxin اسم خاص بيسموه yeast entero + +34 +00:02:37,740 --> 00:02:41,760 +aggregative stable toxin entero aggregative stable + +35 +00:02:41,760 --> 00:02:45,980 +toxin طبعا بينتج عنه من ميكوزل دمج اعتقد المحاضرة + +36 +00:02:45,980 --> 00:02:50,910 +السابقةتحدثنا عن انه طالما ان ال mycosa او ال + +37 +00:02:50,910 --> 00:02:55,810 +mycosal cells او ال epithelial cells ماتت يبقى + +38 +00:02:55,810 --> 00:02:58,090 +مافيش absorption جيد وبالتالي بيصير في + +39 +00:02:58,090 --> 00:03:01,790 +accumulation ل ال electrolytes وعملية اللواء + +40 +00:03:01,790 --> 00:03:06,020 +دياريةاللي هو الحال أمامنا بتوضحلنا ال .. ال .. ال + +41 +00:03:06,020 --> 00:03:08,700 +mechanism by which the five types اللي أحنا + +42 +00:03:08,700 --> 00:03:11,060 +ذكرناهم ال .. ال .. ال entero aggregative و ال + +43 +00:03:11,060 --> 00:03:13,460 +enterotoxinic و ال enteroinvasive و ال + +44 +00:03:13,460 --> 00:03:16,240 +enteropathogenic by .. the mechanism by which they + +45 +00:03:16,240 --> 00:03:19,720 +produce دياري أو infection في ال intestine و + +46 +00:03:19,720 --> 00:03:23,020 +الجدول هذا أيضا هو نفس المعلومات بس ملخصة بشكل + +47 +00:03:23,020 --> 00:03:26,540 +سمري في هذا الجدول مش هنمر عليهم كمان مرة .. مرة + +48 +00:03:26,540 --> 00:03:29,540 +أخرى لكن أنتوا لو طلعتي عليهم هذا بيفيدكوا أنتوا + +49 +00:03:29,540 --> 00:03:34,360 +كنوع من التلخيص للمعلومات اللي ذكرناها بالصحةهذه + +50 +00:03:34,360 --> 00:03:37,300 +اللوحة هنجيها في عينها مطولا وإن كان المحتوى + +51 +00:03:37,300 --> 00:03:40,420 +تباعها أربع خمس كلمات لكن هنجيها في عينها مطولا + +52 +00:03:40,420 --> 00:03:43,720 +لإن احنا بنتكلم عن أو بنختم عن ال Escherichia coli + +53 +00:03:43,720 --> 00:03:48,580 +ذكرناها ك normal microbiota ذكرناها ك outside the + +54 +00:03:48,580 --> 00:03:51,660 +intestinal tract ذكرناها رجعنا ك intestinal tract + +55 +00:03:51,660 --> 00:03:56,760 +pathogen لإن هنرجعلها ك normal microbiota لما كل + +56 +00:03:56,760 --> 00:04:00,080 +البشر بيحتووا في أمعاهم على ال Escherichia coli + +57 +00:04:01,010 --> 00:04:05,650 +معنى الحاجة كل ستول بيحتوي على إشريشيا كولاي، كل + +58 +00:04:05,650 --> 00:04:10,250 +سويج في العالم في ستول يبقى فيه إشريشيا كولاي، + +59 +00:04:10,250 --> 00:04:14,550 +number one contaminant أو contamination source for + +60 +00:04:14,550 --> 00:04:18,150 +drinking water all over the world is sewage يعني + +61 +00:04:18,150 --> 00:04:24,410 +ستول يعني إشريشيا كولايوعليه فقد استخدمت الاشريشيا + +62 +00:04:24,410 --> 00:04:27,210 +كولاي كما يسمى .. أو كما .. كما يطلق عليها + +63 +00:04:27,210 --> 00:04:31,230 +indicator water sanitary indicator ايش يعني + +64 +00:04:31,230 --> 00:04:36,570 +sanitary؟ sanitary نظافة صحة نظافة .. نظافة أكتر + +65 +00:04:36,570 --> 00:04:40,390 +من .. من كلمة صحة لما نقول sanitation يعني مستوى + +66 +00:04:40,390 --> 00:04:45,270 +النظافة sanitation conditions تعالوا + +67 +00:04:45,270 --> 00:04:50,890 +نجيب المجلوب جيبنا عينة ماء اللي جينا فيها اشريشيا + +68 +00:04:50,890 --> 00:04:58,830 +كولايمن ورد يتجيب الإشليشيا كولاي؟ من تلوث بسوج، + +69 +00:04:58,830 --> 00:05:03,450 +من براز آدمي، يبقى أصبحت الإشليشيا كولاي ك + +70 +00:05:03,450 --> 00:05:10,670 +indicator على تلوث الماء أو الغذاء ببراز آدمي طيب + +71 +00:05:10,670 --> 00:05:14,490 +الإشليشيا كولاي اللي بيحددها لو أحنا شربناها، + +72 +00:05:14,490 --> 00:05:18,090 +تمام؟ هل في هناك ضلر منها إذا كانت من ال normal + +73 +00:05:18,090 --> 00:05:22,970 +microbiota؟ مافيش ضلرلكن كمرة أنا ماء أخدتها ك + +74 +00:05:22,970 --> 00:05:26,090 +pathogen أخدتها كمؤشر على ورود ال pathogen بالعربي + +75 +00:05:26,090 --> 00:05:30,010 +البسيط بنقول اللي خلّى ال Escherichia coli تدخل في + +76 +00:05:30,010 --> 00:05:32,710 +الماء بيخلّي ال salmonella بيخلّي ال shigella + +77 +00:05:32,710 --> 00:05:36,350 +بيخلّي ال yersinia enterocolitica تمام؟ لإن المصدر + +78 +00:05:36,350 --> 00:05:41,430 +تبعهم إيه؟ واحد فمن باب الصحة العامة أنا قريبا + +79 +00:05:41,430 --> 00:05:46,230 +للأدهار جبنا لواحد منكم كأس من الماء و قلنا لهم + +80 +00:05:46,230 --> 00:05:49,620 +الله احنا راجينا فيه Escherichia coliالاشيشة كلها + +81 +00:05:49,620 --> 00:05:53,220 +تعني يمكن السلمون اللي موجودة، هيقول له حتى ريمكن + +82 +00:05:53,220 --> 00:05:59,400 +لأ، فضل اشرب من هذا الماءتقدر تشرب النوم؟ ليش؟ لإن + +83 +00:05:59,400 --> 00:06:03,500 +احتمالية .. لإن احتمالية وجود ال salmonella موجودة + +84 +00:06:03,500 --> 00:06:08,700 +فبالتالي احنا من بداية الصحة العامة الوقاية خير من + +85 +00:06:08,700 --> 00:06:13,700 +علاج واحنا كمسئولين عن الصحة العامة نرفض ان الشعب + +86 +00:06:13,700 --> 00:06:18,220 +ياخد مي فيها E.coli يعني فيها fecal contamination + +87 +00:06:18,220 --> 00:06:23,100 +وبالتالي ال E.coli ك water indicator او ك food + +88 +00:06:23,100 --> 00:06:28,290 +quality indicator مهم جدا في هذا الجانبطبعا هناك + +89 +00:06:28,290 --> 00:06:31,630 +مواصفات و مقاييس يعني مثلا احنا بنفحص 100 ملي 100 + +90 +00:06:31,630 --> 00:06:34,810 +ملي من الماء لإنه غالبا الماء بيكونش فيه عدد كبير + +91 +00:06:34,810 --> 00:06:38,190 +من البكتيريا فبن filter 100 ملي على membrane + +92 +00:06:38,190 --> 00:06:43,310 +filter و بنحطهم على وسط selective لل E.coli و بنعد + +93 +00:06:43,310 --> 00:06:49,790 +البكتيري المسموح هو zero مش يعني zero يعني في كل + +94 +00:06:49,790 --> 00:06:54,690 +100 ملي ممنوع يكون فيه ولا E.coli واحدةولا كل form + +95 +00:06:54,690 --> 00:06:57,410 +واحدة، فيه هنا كان عندي standard قديم بيقول إنه في + +96 +00:06:57,410 --> 00:07:01,530 +الألف لو أنا فلترت ألف mill مش ميت ميل، مسموح لحد + +97 +00:07:01,530 --> 00:07:06,410 +تلاتة الألف عشر أضعاف ال .. ال .. ال ميت ميل، صح؟ + +98 +00:07:06,410 --> 00:07:12,770 +فلو جسمنا تلاتة على عشرة، بصي .3، مافيش حاجة اسمها + +99 +00:07:12,770 --> 00:07:16,510 +.3 بكتيريا، يعني طولت بكتيريا، طولت حبة بكتيريا، + +100 +00:07:16,510 --> 00:07:21,840 +مافيش، فالرقم بيكون إيه؟ Zero، بيكون Zeroألخص + +101 +00:07:21,840 --> 00:07:28,160 +الجزء الأخير وجود E.coli في الماء أو في الغذاء + +102 +00:07:28,160 --> 00:07:33,280 +يعتبر مؤشر على مدى .. عشان ما .. ما .. ما .. ما + +103 +00:07:33,280 --> 00:07:38,200 +أفتيش فتوى عامة على مدى نظافة هذا الماء وعدم تلوثه + +104 +00:07:38,200 --> 00:07:42,560 +أو تلوثه ببرازه أخرى طبعا المصطلح اللي جدامي هنا + +105 +00:07:42,560 --> 00:07:47,980 +مش E.coli، كوليفورت فاكرين اللوحة اللي قرضنا فيها + +106 +00:07:47,980 --> 00:07:51,640 +أسماء الـ Enterobacteriasi؟كان فيه تلاتة باللون + +107 +00:07:51,640 --> 00:07:55,160 +البني وكان فيه أربع تحتيهم خط الأربع اللي تحتيهم + +108 +00:07:55,160 --> 00:07:57,060 +خط هم هدول الكوليفورم اللي هم ال lactose + +109 +00:07:57,060 --> 00:08:02,080 +fermenting enterobacterias هم هدول اسمهم كوليفورم + +110 +00:08:02,080 --> 00:08:08,360 +زمان كنا بستخدم هم الكوليفورم ك index كمؤشر للتلوث + +111 +00:08:08,360 --> 00:08:12,400 +الآن يعني فيه توجه ان نستخدم فقط الاشريشيا كولى + +112 +00:08:12,400 --> 00:08:16,420 +لإن هي الأكتر تعبيرا عن التلوث ببراز آدمي أو + +113 +00:08:16,420 --> 00:08:22,640 +حيواني وصل الصورة هذهأرجو ذلك طبعا ليودل على همية + +114 +00:08:22,640 --> 00:08:28,100 +هناك مختبر صحة عامة في قطاع غزة و أكيد فيه في + +115 +00:08:28,100 --> 00:08:32,100 +الضفة الغربية أيضا هذا المختبر في قسم اسمه water + +116 +00:08:32,100 --> 00:08:35,440 +microbiology تسعين بالمية من فحصاته بدوي على + +117 +00:08:35,440 --> 00:08:38,980 +الشريشة كولاي أو على الكوليفور يعني موظفين شخص فقط + +118 +00:08:38,980 --> 00:08:42,520 +يعمل العمل يتبع الفلترة و يحط الفلترة على الشريشة + +119 +00:08:42,520 --> 00:08:48,060 +كولاي و قسم مهم جدا جدا جدا للصحة العامةوعشان احنا + +120 +00:08:48,060 --> 00:08:51,900 +دخلين على ميكروب تاني خدنا نعمل مقدمة أخرة في + +121 +00:08:51,900 --> 00:08:55,440 +الحروب و احنا عصرنا تلات حروب في أقل من ست سنوات + +122 +00:08:55,440 --> 00:09:00,740 +في الحروب الإنسان مقاييسه بتتغير في الغذاء و في + +123 +00:09:00,740 --> 00:09:03,660 +الشراب يعني في الماء حتى يعني بقى جاي يشرب ميه من + +124 +00:09:03,660 --> 00:09:06,220 +فلترة ممكن يشرب ميه من البلدية و ممكن ينزل لمستوى + +125 +00:09:06,220 --> 00:09:11,120 +أقل من ذلك لو الأمور ساءتوهذا كلام متعرف عليه مش + +126 +00:09:11,120 --> 00:09:14,060 +هنا بس فى كل العالم فى الحروب مواصفات الإنسان + +127 +00:09:14,060 --> 00:09:20,180 +بتختلف لإن ظروف الحروب أيضا تختلف مش رفاهية طيب هل + +128 +00:09:20,180 --> 00:09:23,940 +الحروب جانب وكل كوعاصرته أنه كان فى نقص فى المياه + +129 +00:09:23,940 --> 00:09:28,580 +وإنه عملنا إجراءات معينة فى بيوتنا كلتنا حتى نحافظ + +130 +00:09:28,580 --> 00:09:33,690 +على المياه وكل ما طولت الفترة كل ما زادت قصوداللي + +131 +00:09:33,690 --> 00:09:37,810 +هي الظروف هذه كل ما زادت إجراءاتنا وكل ما قلت + +132 +00:09:37,810 --> 00:09:41,050 +مصادر المياه الشروق .. تعالوا نطلع بضارة بتاعنا + +133 +00:09:41,050 --> 00:09:45,470 +قبل سنتين أو سنة الماضية أظن أننا مطر شديد وفي + +134 +00:09:45,470 --> 00:09:48,770 +مناطق غرجت في الغزة غرجت ليش ما اتغرجت يعني .. + +135 +00:09:48,770 --> 00:09:54,270 +يعني انه ال sewage system نظام المجاري تمام لم + +136 +00:09:54,270 --> 00:09:59,610 +يستوعب كميات الأمطار الغزيرة .. يعني المي حار تدخل + +137 +00:10:00,140 --> 00:10:04,180 +الان احتكاك المياه بالمياه موجود الاتصال بين مياه + +138 +00:10:04,180 --> 00:10:08,040 +المطر ومياه المجاري موجود وبالتالي مياه العادمة + +139 +00:10:08,040 --> 00:10:11,240 +أصبحت موردة في مياه الأمطار اللي بيمشي في الشوارع + +140 +00:10:11,240 --> 00:10:16,340 +اللي انتشرت ووصلت إلى أماكن لم تكن لتصلها المياه + +141 +00:10:16,340 --> 00:10:20,020 +العادمة معناه الحكي أن إمكانية التلوث في هذا + +142 +00:10:20,020 --> 00:10:26,370 +الحالة عالية جدانيجيلا قبل فترة كان فيه tsunami في + +143 +00:10:26,370 --> 00:10:29,470 +اندونسيا عارفين ال tsunami اللي هي الموجة العالية + +144 +00:10:29,470 --> 00:10:36,310 +من المياه اللي غمرت مناطق كبيرة و قتلت ألاف من + +145 +00:10:36,310 --> 00:10:40,490 +الناس في تلك الفترةعلى طول بعدها بيوم أو يومين + +146 +00:10:40,490 --> 00:10:44,090 +بدأت تظهر تقارير من ال WHO تقول هناك خشية من تفشي + +147 +00:10:44,090 --> 00:10:47,730 +الأوبئة نظرا لعدم توفر مياه الشرب الأمنة، إذا مياه + +148 +00:10:47,730 --> 00:10:53,130 +الشرب الأمنة هي مطلب مش شخصي، مطلب مجتمعي، لذلك + +149 +00:10:53,130 --> 00:10:57,330 +الدولة مسئولة عن توفير .. الدولة مسئولة عن توفير + +150 +00:10:57,330 --> 00:11:01,570 +مياه أمنة، عندنا في فلسطين .. في قطاع غزة المصدر + +151 +00:11:01,570 --> 00:11:06,800 +أساسي لمياه الشرب هو اللي هو المياه الجوفيةلكن + +152 +00:11:06,800 --> 00:11:09,460 +المياه الجيوفية حتى المياه الجيوفية مش مركزية + +153 +00:11:09,460 --> 00:11:13,140 +بمعنى أنه كلها مصدر واحد وكل الناس بتاخد منها من + +154 +00:11:13,140 --> 00:11:17,840 +مصدر واحد، في ناس عندهم قبار خاصة فيهم، في منهم + +155 +00:11:17,840 --> 00:11:21,760 +legal وفي منهم illegal، يعني ماخدين ترخيص وفي ناس + +156 +00:11:21,760 --> 00:11:24,700 +ماخدين مش ماخدين ترخيص، في ناس بيفحصوا المياه + +157 +00:11:24,700 --> 00:11:28,020 +تبعتهم وفي ناس مافحصوش المياه تبعتهم، يعني الرقابة + +158 +00:11:28,020 --> 00:11:31,420 +على المياه في قطاع غزة في أي في عليه عليه بتهيأت، + +159 +00:11:31,420 --> 00:11:36,490 +استفهاملكن على مستوى الباديات هناك system لمراقبة + +160 +00:11:36,490 --> 00:11:40,470 +المياه اللى تصل للبيوت و لو عملنا مقارنة ما بين + +161 +00:11:40,470 --> 00:11:46,410 +المياه اللى من البلدية و المياه اللى بتشتروها من + +162 +00:11:46,410 --> 00:11:50,630 +البياعين المياه اللى .. المياه المحلقة بتسميها ولا + +163 +00:11:50,630 --> 00:11:54,650 +مفلطقة هتلاقوا أن مستوى النظافة تبعها البلدية أعلى + +164 +00:11:54,650 --> 00:11:58,770 +من ال .. صحيح الملوحة أسوأ .. أه سيئة الملوحة لكن + +165 +00:11:58,770 --> 00:12:07,440 +مستوى النظافةاستخدع العملية الكلورة كل ما ذكرته من + +166 +00:12:07,440 --> 00:12:13,240 +المقدمات هو ان التلوث المياه او مياه الشرب واللي + +167 +00:12:13,240 --> 00:12:18,900 +المؤشر عليه الشريشة كولاي او الكوليفان خطر كبير + +168 +00:12:18,900 --> 00:12:24,500 +جدا على الصحة العامة ايش المخاطر؟ احد المخاطر حضرة + +169 +00:12:24,500 --> 00:12:24,920 +مين؟ + +170 +00:12:27,540 --> 00:12:31,560 +شي جلّا فيه أربع أسماء سهلين مش زي ال .. + +171 +00:12:31,560 --> 00:12:33,460 +السيمونيلا .. السيمونيلا فيها أسماء كتيرة الصحيح + +172 +00:12:33,460 --> 00:12:36,820 +..شي جلّا أربع فقط ..شي جلّا فليكسنيري ..شي جلّا + +173 +00:12:36,820 --> 00:12:39,600 +بويدي ..شي جلّا سنناي ..شي جلّا ديزنتيري .. + +174 +00:12:39,600 --> 00:12:44,640 +الأخيرة هي الأهمDysentery من دوزونطاريا من + +175 +00:12:44,640 --> 00:12:47,480 +dysentery باللغة الإنجليزية و dysentery اللي انا + +176 +00:12:47,480 --> 00:12:49,560 +وضحتلكوا هي اخدوا بالكم المحاضرة اللي ماضية لما + +177 +00:12:49,560 --> 00:12:56,640 +قلتلكوا انه دياريا باس ميوكاس زي اللي هو blood هذه + +178 +00:12:56,640 --> 00:13:00,880 +الأربعة اتوفرت في ال characteristics لبرازي و هم + +179 +00:13:00,880 --> 00:13:06,300 +نسميها dysenteryال dysentery كان بتسببه كمان مش + +180 +00:13:06,300 --> 00:13:09,680 +كان، اللي هتلعب بتسببه ال amoeba اللي اسمها + +181 +00:13:09,680 --> 00:13:12,600 +intamoeba histolytica، درستوها في التانوية العامة + +182 +00:13:13,460 --> 00:13:15,900 +أو بالثانوية اللي قدرت عليها العامة و لا قبل ذلك + +183 +00:13:15,900 --> 00:13:20,400 +اسمها Entamoeba Histerica و بتعمل dysentery فعشان + +184 +00:13:20,400 --> 00:13:25,380 +نميز ال dysentery تبع الشجلة عن ال dysentery تبع + +185 +00:13:25,380 --> 00:13:30,060 +ال .. ال Entamoeba سموها دي Amoeba dysentery و + +186 +00:13:30,060 --> 00:13:33,180 +هيسموها Bacillary Bacillary من شكل البكتيريا + +187 +00:13:33,180 --> 00:13:36,380 +البسلية اللي هو العصرية Bacillary dysentery إذا + +188 +00:13:36,380 --> 00:13:39,260 +المفهوم اللي ريجى اسم Bacillary dysentery عشان + +189 +00:13:39,260 --> 00:13:44,010 +نميزها عن ال Amoeba dysenteryبيطلق عليه بعض + +190 +00:13:44,010 --> 00:13:47,470 +الأحيان Shigellosis وده أسهل تسمية Shigella, + +191 +00:13:47,530 --> 00:13:50,350 +Shigellosis, Salmonella, Salmonella's لكن للأسف مش + +192 +00:13:50,350 --> 00:13:55,270 +دايما الأسماء وردية بهذا الطريقة it produces + +193 +00:13:55,270 --> 00:14:00,710 +bloody feces، براز مدمّم، intestinal pain، زائد + +194 +00:14:00,710 --> 00:14:05,350 +اللي هو ال pass وكنا تلقائيا فيه amucus من ال + +195 +00:14:05,350 --> 00:14:08,490 +general features إنها بقالها بالعي most strains + +196 +00:14:08,490 --> 00:14:12,270 +cannot ferment lactose وهنا بنكبر شوية المحاضرة + +197 +00:14:17,740 --> 00:14:20,780 +lactose fermenter يعني يا بيض يا أسود يا بتخمر يا + +198 +00:14:20,780 --> 00:14:26,620 +مابتخمرش اليوم بدنا نعرف حالتان slow أو بيسميها + +199 +00:14:26,620 --> 00:14:30,080 +احنا مصطلح قدق اسمها delayed lactose fermenter + +200 +00:14:30,080 --> 00:14:36,480 +delayed lactose fermenter يعني بتخمرش ال lactose + +201 +00:14:36,480 --> 00:14:43,240 +within 24 hours بدها اكتر من 24 hours طب انا كنت + +202 +00:14:43,240 --> 00:14:47,700 +يعني حازمفي المحاضرة قلت lactose fermenter و non + +203 +00:14:47,700 --> 00:14:51,760 +lactose fermenter هل هناك سبب ان انت راجعت و قلت + +204 +00:14:51,760 --> 00:14:56,940 +ان هم تلاتة delayed و non lactose و lactose تعالوا + +205 +00:14:56,940 --> 00:15:02,520 +نفهم الموضوع فهم اعمق شوية اللي دخل المختبر ال + +206 +00:15:02,520 --> 00:15:06,790 +microbiology اكيدشاف المكونكي حتى وإن ماعرفش إنه + +207 +00:15:06,790 --> 00:15:11,390 +مكونكي بس أكيد شفته لإن الكليبسيلة اللي نامية على + +208 +00:15:11,390 --> 00:15:16,350 +الطبق اللي لونها حمرا و ميكوين تمام أو الاشيشيا + +209 +00:15:16,350 --> 00:15:18,930 +كلها اللي لونها أحمر و ميكوين هذا المكونكي + +210 +00:15:18,930 --> 00:15:24,990 +المكونكي بنزل عليه البكتيريا لغرض إنه يمنع الإجرام + +211 +00:15:24,990 --> 00:15:27,770 +بُسطه و يسمح للإجرام نيجاتيه فهو selective for + +212 +00:15:27,770 --> 00:15:32,040 +إجرام نيجاتيه و differentialبيميز بين ال lactose + +213 +00:15:32,040 --> 00:15:34,920 +fermenter و ال non lactose fermenter كيف؟ في + +214 +00:15:34,920 --> 00:15:39,940 +lactose و في indicator لل pH لما زرعنا البكتيريا + +215 +00:15:39,940 --> 00:15:44,260 +من النمى ل gram نيجاتيف ال gram نيجاتيف بنفترض + +216 +00:15:44,260 --> 00:15:47,660 +الآن يا إما lactose fermenter يا إما non lactose + +217 +00:15:47,660 --> 00:15:52,560 +fermenter إذا lactose fermenter هتعمل fermentation + +218 +00:15:52,560 --> 00:15:59,030 +ل lactoseو ال result هو acid ال acid هيخفض ال pH و + +219 +00:15:59,030 --> 00:16:02,430 +ال pH indicator هيتغير لونه إلى اللون الأحمر فبصير + +220 +00:16:02,430 --> 00:16:05,790 +انك شفت مستعمرة حمرة على المكونكي فهي lactose + +221 +00:16:05,790 --> 00:16:10,730 +fermenter على النقيد اذا ما خمرت ال lactose مافي + +222 +00:16:10,730 --> 00:16:13,550 +acid مافي تغيير ال pH indicator حتى لو المستعمرة + +223 +00:16:13,550 --> 00:16:19,170 +clearness او ماخدة اللون الوسطى شفافة يعني اذا + +224 +00:16:19,170 --> 00:16:21,410 +lactose fermenter لما lactose fermenter طب وين + +225 +00:16:21,410 --> 00:16:24,470 +الدليل lactose fermenterواحدة lactose ferment .. + +226 +00:16:24,470 --> 00:16:27,590 +non-lactose fermenter لو طلعت الطبق على تلت أيام، + +227 +00:16:27,590 --> 00:16:31,310 +أربع أيام ممكن تتغير لغنها و تصل لغنها بس أنا + +228 +00:16:31,310 --> 00:16:33,830 +حقيقة لما بقرا الأطباق بقراهاش بعد تلت أيام بقراها + +229 +00:16:33,830 --> 00:16:35,670 +بعد أربع و عشرين ساعة أو أقل حتى من أربع و عشر + +230 +00:16:35,670 --> 00:16:38,770 +ساعة وبالتالي practically أنا بصنف ال .. + +231 +00:16:38,770 --> 00:16:41,790 +practically بصنف البكتيريا إلى lactose و non + +232 +00:16:41,790 --> 00:16:46,440 +-lactose ك practical لكن كواقع they arethree + +233 +00:16:46,440 --> 00:16:50,300 +lactose و non lactose و في النص delayed ال delayed + +234 +00:16:50,300 --> 00:16:53,600 +و ال non lactose بنصنفهم على انهم non lactose كمان + +235 +00:16:53,600 --> 00:16:56,540 +مرة for practical reasons ان احنا بنقل أطباق بعد + +236 +00:16:56,540 --> 00:17:02,800 +أقل من 24 ساعة وصلت الصورة لكن ممكن نستفيد من + +237 +00:17:02,800 --> 00:17:04,980 +lactose و delayed lactose fermenter و non lactose + +238 +00:17:04,980 --> 00:17:12,280 +fermenter في بعض الفحوصات لما نشتغل ال ABI مثلا كل + +239 +00:17:12,280 --> 00:17:16,500 +ال lactose fermenterو ال delayed lactose fermenter + +240 +00:17:16,500 --> 00:17:21,360 +بيكونوا positive لل ONBG ONBG فحص ماعرفش مش حد + +241 +00:17:21,360 --> 00:17:24,400 +هتندش نقعد نشرح فيه لكن ال non-lactose fermenter + +242 +00:17:24,400 --> 00:17:28,640 +بيكونوا negative فمين ال .. ال .. يعني بمعنى أخر + +243 +00:17:28,640 --> 00:17:31,580 +بقدر أميز بين يعني لو بكتيريا طلعت لي non-lactose + +244 +00:17:31,580 --> 00:17:36,060 +على الطبق و طلعت ONBG positive مش معناها معناها + +245 +00:17:36,060 --> 00:17:41,080 +delayed lactose fermenter لكن لو طلعت حمرة lactose + +246 +00:17:41,080 --> 00:17:44,960 +fermenter برضه هطلعش لأنهاومبجي بسطف، يعني مين + +247 +00:17:44,960 --> 00:17:48,320 +اللي .. مين اللي بيطلع بسطف في ال OMBG؟ ال delayed + +248 +00:17:48,320 --> 00:17:52,340 +و ال lactose fermenter، بينما ال true non-lactose + +249 +00:17:52,340 --> 00:17:57,500 +fermenter بيطلع negative في ال OMBG طيب، هذا + +250 +00:17:57,500 --> 00:18:01,880 +الكلام حكيناه كله عشان خاطر كلمة إنه في شي جلة + +251 +00:18:01,880 --> 00:18:06,340 +صنعي can ferment lactose slowly وسمينها delayed + +252 +00:18:06,340 --> 00:18:09,710 +lactose fermentAccording to O Antigen, they are + +253 +00:18:09,710 --> 00:18:12,230 +classified into four groups، والـ four groups هما + +254 +00:18:12,230 --> 00:18:16,850 +حقيقة كل species منهم group لحالة، كل species طلعت + +255 +00:18:16,850 --> 00:18:20,690 +group لحدها، can easily acquire drug resistance، + +256 +00:18:20,690 --> 00:18:24,630 +زيها زي إيه انتروبكترياسي عند المقدرة إنها تكتسب + +257 +00:18:24,630 --> 00:18:30,500 +مقاومةال average .. ال average لل .. لل .. لل + +258 +00:18:30,500 --> 00:18:34,060 +incubation period كلمة average مش معناه تحكي أنه + +259 +00:18:34,060 --> 00:18:38,260 +يعني ممكن تكون من واحد لسبع أيام لكن most أو معظم + +260 +00:18:38,260 --> 00:18:41,360 +الناس من اتنين لتلت أيام فما .. هي .. هي أطول من + +261 +00:18:41,360 --> 00:18:46,780 +ذلك يعني لكن هي من واحد إلى سبعة و ممكن معظم الناس + +262 +00:18:46,780 --> 00:18:50,000 +تكون في مرحلة اتنين لتلت أيام و تم ال bacterial + +263 +00:18:50,000 --> 00:18:55,670 +cell damageواشيقلة قبل ما يعني نخد فيه شوية تفاصيل + +264 +00:18:55,670 --> 00:19:01,650 +بدا نذكركوا انه rarely isolated from sites other + +265 +00:19:01,650 --> 00:19:06,390 +than the intestinal tract ايش يعني؟ نادرا ما عزلت + +266 +00:19:06,390 --> 00:19:09,730 +من اماكن غير ال intestinal tract يعني بتعمل + +267 +00:19:09,730 --> 00:19:13,310 +localized infection مابتعملش invasive او systemic + +268 +00:19:13,310 --> 00:19:17,530 +infection هي بتعمل invasion فقط لطبقة رقيقة من ال + +269 +00:19:17,530 --> 00:19:23,510 +v-line اما مابتقفلش ولا اداركيبقى هي مش systemic + +270 +00:19:23,510 --> 00:19:25,930 +disease تم ال localize infection في ال main في ال + +271 +00:19:25,930 --> 00:19:29,830 +intestinal tract كلكم بتعرفوا أن ال toxin تبعها + +272 +00:19:29,830 --> 00:19:34,190 +بيعمل destruction لل .. لل .. لل .. لل .. لل + +273 +00:19:34,190 --> 00:19:38,370 +ribosome ال RNA اللي بيشكل ال ribosome .. بشكل ال + +274 +00:19:38,370 --> 00:19:41,690 +ribosome بدون ribosome مافي عندنا protein + +275 +00:19:41,690 --> 00:19:45,370 +synthesis واتفقنا protein synthesis بيقضي على .. + +276 +00:19:45,370 --> 00:19:49,290 +إذا توقف تماما بيقضي على الخبر ويوصف ال toxin بأنه + +277 +00:19:49,290 --> 00:19:53,630 +enterotoxicأو cytotoxic ليش enterotoxic؟ لأنه ال + +278 +00:19:53,630 --> 00:19:58,170 +effect تبعه في ال intestine لكن cytotoxic لأنه + +279 +00:19:58,170 --> 00:20:01,310 +برضه يتأثير على كل .. يعني إذا وصل أي خلية بيعملها + +280 +00:20:01,310 --> 00:20:05,390 +destruction فهو generalized effect تمام لكن من حصل + +281 +00:20:05,390 --> 00:20:08,170 +حظنا أنه .. أنه confined في وين؟ في ال intestinal + +282 +00:20:08,170 --> 00:20:15,030 +tract هذه المعلومة نفس المكررة شوفوا في إمكانية .. + +283 +00:20:15,030 --> 00:20:18,530 +طلعوا أنا على اللوحة دي معلشالبكتيريا برضه انت + +284 +00:20:18,530 --> 00:20:21,910 +type 3 secretion system فاكرينه اللي قلنا عنه اللي + +285 +00:20:21,910 --> 00:20:25,190 +بيوحي للبكتيريا بإنه انت phagocyte وإنه + +286 +00:20:25,190 --> 00:20:31,330 +phagocytized the بكتيريا و جوا بيمنع ارتباط ال + +287 +00:20:31,330 --> 00:20:35,930 +lyosome بال phagosome و بتتكاتر البكتيرياواضح أن + +288 +00:20:35,930 --> 00:20:39,690 +البكتيريا عندها المقدرة على الانتقال laterally إيش + +289 +00:20:39,690 --> 00:20:43,330 +يعني laterally؟ يعني بدل ما تدمر الخلية وتروح + +290 +00:20:43,330 --> 00:20:46,710 +وتعمل نفس ال infection لأ ممكن تنتقل من خلية إلى + +291 +00:20:46,710 --> 00:20:51,930 +خلية مباشرة برضه عبر ال actin rackets الactin + +292 +00:20:51,930 --> 00:20:56,190 +rackets اللي هي قلنا الصواريخ الناتجة عن ال + +293 +00:20:56,190 --> 00:21:00,570 +polymerization and depolymerization يعني بلمرة و + +294 +00:21:00,570 --> 00:21:03,850 +إعادة تفكيك ل .. اللي هو ال .. و أنا قلتلكوا + +295 +00:21:03,850 --> 00:21:07,690 +هحطلكوا صورةأبحث لكوا فيديو بيوضّح حاجة طريقة + +296 +00:21:07,690 --> 00:21:11,750 +رائعة جدا في ال Listeria Monocytogenes مصورة تحت + +297 +00:21:11,750 --> 00:21:15,130 +ال microscope صورة حقيقية وطبيعية كيف ال Listeria + +298 +00:21:15,130 --> 00:21:21,570 +بتنتقل من خلية إلى خلية أخر، بالأكتينراكيتز ال + +299 +00:21:21,570 --> 00:21:26,090 +clinical significance لشجلة man is the only + +300 +00:21:26,090 --> 00:21:30,990 +reservoir man أقصد فيها لمين كان مرة الإنسانشفت + +301 +00:21:30,990 --> 00:21:36,710 +الصورة الموجودة على الجانب اللي بيعطي مؤشر سهم من + +302 +00:21:36,710 --> 00:21:42,590 +البراز إلى الفم مشهورة اسمها fecal oral و بالعربي + +303 +00:21:42,590 --> 00:21:47,730 +بدون أي تجميل من البراز إلى الفم هل من البراز إلى + +304 +00:21:47,730 --> 00:21:54,550 +الفم حاجة شائعة أكتر حاجة شائعة في البلد العالم + +305 +00:21:54,550 --> 00:21:58,310 +الثالث لانتقال الأمراض هي من البراز إلى الفم + +306 +00:21:58,310 --> 00:22:03,180 +لماذا؟انتوا بتنسوا بسرعة لسه مانعش عشرة دقاق نتكلم + +307 +00:22:03,180 --> 00:22:07,580 +على انه أكبر مصدر ملوث لمياه الشرق في العالم هو ال + +308 +00:22:07,580 --> 00:22:09,600 +.. هو ال sewage و ال sewage بيحتاجوا عليها ال + +309 +00:22:09,600 --> 00:22:12,340 +stool ال stool اللي هو البراس تمام؟ وبالتالي + +310 +00:22:12,340 --> 00:22:16,840 +إمكانية انتقال الميكروبات و أكبر درجة لذلك .. أكبر + +311 +00:22:16,840 --> 00:22:21,300 +درجة لذلك ان نسبة الأمراض ال .. ال .. ال .. ال .. + +312 +00:22:21,300 --> 00:22:24,120 +ال protozoa و اللي تنتقل على طريق الفكرة الأولى + +313 +00:22:24,120 --> 00:22:26,960 +العالية عندنا في فلسطين وبالتالي احنا عندنا هذه ال + +314 +00:22:26,960 --> 00:22:31,170 +.. ال .. ال .. ال ..ال mood of when بدأت تقل و + +315 +00:22:31,170 --> 00:22:35,710 +بدأت تقل بإيه؟ بتحسن ال infrastructure تبع ال + +316 +00:22:35,710 --> 00:22:39,450 +sewage إنه صار فينا closed sewage system نظام + +317 +00:22:39,450 --> 00:22:43,110 +مجاري مغلق و أنا بقولكوا إنه مش كل المناطق مغطاة + +318 +00:22:43,110 --> 00:22:46,690 +يعني مش hundred percent الناس كلها مغطاين لأ في + +319 +00:22:46,690 --> 00:22:51,100 +مناطقلسه ماوصلهاش ال sewage system وبيستخدموا + +320 +00:22:51,100 --> 00:22:55,280 +القبار اللي هي الشخصية يعني ان كل واحد بيحفرله + +321 +00:22:55,280 --> 00:22:58,860 +حفرة في البيت حفرة إدمصاصية بيسموها septic tank + +322 +00:22:58,860 --> 00:23:03,140 +حفرة إدمصاصية بيتخلص فيها من اللي هو ال waste + +323 +00:23:03,140 --> 00:23:09,020 +water تبعه أكتر ناس عرضة للإصابة اللي هم ال young + +324 +00:23:09,020 --> 00:23:13,400 +children فيكل تقرأ ال contact زي ما حكينا children + +325 +00:23:13,400 --> 00:23:16,040 +to adult طبعا لما يكون الطفل برضه مصاب في البيت + +326 +00:23:16,040 --> 00:23:19,800 +ممكن ينقلها الأخرينالنقطة المهمة جدا اللي هي + +327 +00:23:19,800 --> 00:23:22,980 +transmitted by adult food handlers الصورة التانية + +328 +00:23:22,980 --> 00:23:28,820 +على جانب كان ناس كانوا بدخن اظنني وشكلهم يعني مش + +329 +00:23:28,820 --> 00:23:35,520 +انضاف لكن جدهم طعوا و بحضروا الناية انتبهوا ليه + +330 +00:23:35,520 --> 00:23:40,580 +بقى سأقول الآن عملية + +331 +00:23:40,580 --> 00:23:45,080 +تحضير الغداء عملية تحضير الغداء هي بمثابة تحضير + +332 +00:23:45,080 --> 00:23:53,970 +يعني اما هديةطعام صحي أو قنبلة موبوقة لأن .. و + +333 +00:23:53,970 --> 00:23:56,590 +يعتبر على مين الشخص اللي بيحضر و فاكرين جثة + +334 +00:23:56,590 --> 00:23:59,550 +tayfoyd mallet؟ tayfoyd mallet لم تغيب على الاذهان + +335 +00:23:59,550 --> 00:24:04,490 +طيب نتخيل أنه في حد في الكفتيريا عندنا في الجامعة + +336 +00:24:04,490 --> 00:24:10,070 +بيشتغل بنظرية tayfoyd mallet طبعا؟ طيب مين فيكوا + +337 +00:24:10,070 --> 00:24:13,450 +بيروح الكفتيريا؟ ولا حد الحين بطل على البطل يبقى + +338 +00:24:13,450 --> 00:24:17,850 +بيعمل صندوق شاط من البيت طيبطبعا احنا في الجامعة + +339 +00:24:17,850 --> 00:24:22,530 +عندنا رقابة إدارية محترمة جدا على الكفتيريات بتفتش + +340 +00:24:22,530 --> 00:24:27,510 +تفتيش دقيق وهذا اللي بيد إلى التطور اللي حاصل فيه + +341 +00:24:27,510 --> 00:24:35,490 +الكفتيري في قبل 15 سنة عملنا دراسة على مستوى + +342 +00:24:35,490 --> 00:24:40,600 +النظافة المقدمة فيالكفتيريا وعملنا يوم دراسي على + +343 +00:24:40,600 --> 00:24:43,680 +هذا الموضوع و .. و .. و دعونا المسؤولين في الجامعة + +344 +00:24:43,680 --> 00:24:47,740 +لحضور .. و بي .. و أبرزنا بعض نقاط الضعف في النظام + +345 +00:24:47,740 --> 00:24:50,800 +و من .. يعني من تقريبا من هذا الطريق إلى اليوم و + +346 +00:24:50,800 --> 00:24:55,020 +هناك بالفعل لجان مشكلة و فيه رقابة و أنا كمان بره + +347 +00:24:55,020 --> 00:25:00,380 +بأبريكو الرقابة يعني وجودها مهم جدا في أي عمل في + +348 +00:25:00,380 --> 00:25:04,240 +الدنيا مافيش رقابة إذا تركنا الأمر للأشخاص غالبا + +349 +00:25:04,240 --> 00:25:07,420 +أشخاص يميلوا إلى التراخي و من ثم الإهمال زي واحد + +350 +00:25:07,420 --> 00:25:11,600 +بيقعد في المختبروماحدش عليه رقيب يقوله إلبس gloves + +351 +00:25:11,600 --> 00:25:15,000 +أو إلبس المريول أو تعامل بالacid technique بعد + +352 +00:25:15,000 --> 00:25:18,120 +فترة باللجيئة بطل يشتغل هذا الشغل الحالة بقولك يا + +353 +00:25:18,120 --> 00:25:20,900 +عم أنا هنا إلا عشرين سنة بشتغل ولا مقصر بمرض ولا + +354 +00:25:20,900 --> 00:25:25,560 +مقنقل لأحد مرض لكن واقع الأمر عملية المراقبة أمر + +355 +00:25:25,560 --> 00:25:34,200 +ضرب كل ستة أشهر عمال الكفتريات بيخضعوا لفحوصات + +356 +00:25:34,200 --> 00:25:38,460 +بوراز عشان نضمن خلوهم من السمنة والشجرة + +357 +00:25:41,380 --> 00:25:48,760 +تمام؟ هناك تفتيش على الأضافة .. تفتيش على الجروح + +358 +00:25:48,760 --> 00:25:53,060 +.. إذا كان فيه جروح في الإيد .. تمام؟ كمان شغلة .. + +359 +00:25:53,060 --> 00:25:55,520 +بس يمكنها مش بتكون متطبقة .. لكن في المطبخ .. جوا + +360 +00:25:55,520 --> 00:25:57,800 +في المطبخ ممكن يكون متطبقة .. اللي هو الشعر .. و + +361 +00:25:57,800 --> 00:26:01,320 +تقطاته .. يعني من أن يكون الشعر مغطى .. و إذا كان + +362 +00:26:01,320 --> 00:26:04,440 +حتى كمان في لحياة .. يجب أن أيضا كمان تغطى .. لأن + +363 +00:26:04,440 --> 00:26:09,310 +الشعر من الأشياء اللي ممكن تتساقط ..و هي من + +364 +00:26:09,310 --> 00:26:12,370 +المناطق الخارجية في البسمة وبالتالي أكتر عرضة + +365 +00:26:12,370 --> 00:26:16,950 +للتلوث من المناطق الأخرى فبالتالي بيكون هناك نوع + +366 +00:26:16,950 --> 00:26:23,110 +من الرقابة على أي واحد بيعمل في مؤسسة و بيسميها + +367 +00:26:23,110 --> 00:26:26,630 +food handler food handler قد يكون طباخ قد يكون + +368 +00:26:26,630 --> 00:26:31,470 +موزع غذاء زي جرسون او البياع اللي بيبيع الغذاء + +369 +00:26:31,470 --> 00:26:34,270 +نفسه على ال counter هذا اللي بيسميهم food handler + +370 +00:26:34,270 --> 00:26:38,430 +طالما هم لمسوا هذا الغذاءإيه دي بقى نفتح لهم رقابة + +371 +00:26:38,430 --> 00:26:43,550 +دورية و رقابة جيدة حتى نضمن أن .. لأنه نتكلم على + +372 +00:26:43,550 --> 00:26:47,230 +عشرين ألف طالب و طالب في الجامعة أو في الميثال أو + +373 +00:26:47,230 --> 00:26:51,210 +في مؤسسة أو مطعم أو في عزومة أو whatsoever كل هذه + +374 +00:26:51,210 --> 00:26:56,830 +الأشياء ممكن تؤدي إلى انتشار مرض بشكل جماعي إذا لم + +375 +00:26:56,830 --> 00:27:02,370 +نقم نحن بالعمل الصعب unwashed hand + +376 +00:27:06,650 --> 00:27:10,230 +طبعا البعض بيستهدر بهذا الموضوع كثير و بيظن ان + +377 +00:27:10,230 --> 00:27:14,690 +عملية غسل الأيديان هي يعني عملية ما انا اشبه + +378 +00:27:14,690 --> 00:27:19,230 +المسألة في حياتي عشان انا اغسل أيدي باستمرار انا + +379 +00:27:19,230 --> 00:27:21,630 +بدي اقولكم كمان مرة مش لأ كمان مرة تغمضوا عينيكوا + +380 +00:27:21,630 --> 00:27:25,390 +في البيت و تتخيلوا يديكوا هدولة تم تم راحوا و + +381 +00:27:25,390 --> 00:27:29,230 +تخيلوا ايش الأشياء اللي انت كنت تعمليها بالأصابع + +382 +00:27:29,230 --> 00:27:33,830 +لحالهم أصابع هدولة الآن مش هتقدر تعمليهم تقريبا + +383 +00:27:34,680 --> 00:27:39,800 +يمكن بعد خمس دقائق من التفكير هتسود الدنيا و تفقد + +384 +00:27:39,800 --> 00:27:43,200 +الأمل في الحياة و تبطل من الجامعة و تولد في بيته و + +385 +00:27:43,200 --> 00:27:47,320 +بس خمس دقائق من التفكير بإن هدولها مش موجودين + +386 +00:27:47,320 --> 00:27:50,760 +حياتنا الآن مربوطة بال keyboard و بال touch و + +387 +00:27:50,760 --> 00:27:54,480 +بالمقبض و بالفلوس و بنعطي بنمسك الأكل و بنتناول + +388 +00:27:54,480 --> 00:27:58,980 +فيها لو تخيلتوا الأمر الإيدان هم الأداة الأكتر + +389 +00:27:58,980 --> 00:28:03,260 +استخداما على الإطلاق في أجسامهم هم الأداة الأكتر + +390 +00:28:03,260 --> 00:28:08,320 +استخدامافي وقت المقابل بيته حتى في الإيماءات لما + +391 +00:28:08,320 --> 00:28:12,320 +نتكلم و نتحرك نتحرك إيدينا و نقول لها واحد اتنين + +392 +00:28:12,320 --> 00:28:16,060 +هناك يعني المعنى الحاكي أن هدول الأداة الأكثر + +393 +00:28:16,060 --> 00:28:20,500 +استخداما فهي الأداة الأكثر عرضة للتلوث وبالتالي + +394 +00:28:20,500 --> 00:28:25,680 +عملية غسل الإيدين هي عملية مهمة و أذكركم بقص + +395 +00:28:25,680 --> 00:28:30,340 +الأضافة و أذكركم بهيئة وضوء الرسول صلى الله عليه و + +396 +00:28:30,340 --> 00:28:36,630 +سلمكان يكفئ الإناء فيقصل يديه ثم يغرفك، عم يتغسل + +397 +00:28:36,630 --> 00:28:40,470 +إيه؟ هيئة وضوء الرسول صلى الله عليه وسلم بإن كان + +398 +00:28:40,470 --> 00:28:47,210 +يخلل ما بين الأصابع ويدلقتدلك عملية تنظيف لو تطلع + +399 +00:28:47,210 --> 00:28:51,090 +على غسل الأيدان بالطريقة الحالية هتلاقي أنه كل + +400 +00:28:51,090 --> 00:28:54,170 +الأطباء بيعملوا scrubbing لإيديهم وفي مناطق محددة + +401 +00:28:54,170 --> 00:28:59,110 +من الأيدين خاصة القريبة من الأظاثر بتكون قادرة جدا + +402 +00:28:59,110 --> 00:29:02,190 +لأن هم ده ال tips اللي احنا بنلمس فيهم تقريبا كل + +403 +00:29:02,190 --> 00:29:09,150 +شيء هناك مدعاة أنه احنا نغسل إيدينا باستمرار + +404 +00:29:09,750 --> 00:29:13,210 +المسؤال لو انا اشتغل في مستشفى او مختبط و بدي اغسل + +405 +00:29:13,210 --> 00:29:17,510 +يدي باستملار بعد كل حاجة اعملها معناه حاجة حروح + +406 +00:29:17,510 --> 00:29:21,850 +منهم اليدين هيخلصوا دي من الغسيل مظبوط و مش مظبوط + +407 +00:29:21,850 --> 00:29:25,550 +و بيصيروا ال dryness اللي بتعرض لفترة طويلة و + +408 +00:29:25,550 --> 00:29:29,410 +بيصيروا cracked و بيصيروا اكتر عرض يعني الغسيل زاد + +409 +00:29:29,410 --> 00:29:33,650 +من امكانية انهم يمرضوا بدل ما يكونوا صحيين لذلك + +410 +00:29:33,650 --> 00:29:37,790 +يجب ان احرص على الا اغسل يديمنارة و تكرار في + +411 +00:29:37,790 --> 00:29:42,210 +المختبر كيف بدي أحصل على هذا الكلام؟ لأ لأ أنا + +412 +00:29:42,210 --> 00:29:45,550 +هتكلم عن ال gloves كمان شوية ولا ال gel كمان بدي + +413 +00:29:45,550 --> 00:29:51,210 +أتصرف .. أتصرف .. تمام؟ to minimize contacts .. + +414 +00:29:51,210 --> 00:29:56,410 +تمام؟ contacts مع ال hands بقى يعني مثلا الآن قرن + +415 +00:29:56,410 --> 00:30:01,170 +الجوال و أنا قاعد بشتغل ب gloves و لو بدي أضطر + +416 +00:30:01,170 --> 00:30:03,510 +أشله ال gloves كمان و أردع التليفون .. ليش أردع + +417 +00:30:03,510 --> 00:30:08,170 +التليفون؟ ممنوع أردع التليفون في المختبرتمام؟ + +418 +00:30:08,170 --> 00:30:12,070 +ممنوع أمسك جلم و أكتب فيه notes و أنا لابس ال + +419 +00:30:12,070 --> 00:30:15,550 +gloves لإن بعد شوية هشلح ال gloves و أغسل إيديه + +420 +00:30:15,550 --> 00:30:18,650 +تمام؟ و أرجع أمسك مين؟ الجلم و أحطه في الشنطة و + +421 +00:30:18,650 --> 00:30:21,810 +أروح فيها الضغط و .. و .. و .. و إيه خوتك الزغار + +422 +00:30:21,810 --> 00:30:26,950 +ولا حد تاني من الضغط أخد الجلم؟ يعني نجبنا ال .. + +423 +00:30:26,950 --> 00:30:31,390 +ال .. ال microbe من وين؟ من المستشفى فبدنا نصير + +424 +00:30:31,390 --> 00:30:35,740 +نفكر بطريقة لما بقولكوا في المختبرaseptic + +425 +00:30:35,740 --> 00:30:40,100 +technique و لما بقولكوا في المختبر الإنسان سلوكه + +426 +00:30:40,100 --> 00:30:44,220 +بيختلف عن سلوكه في الشارع بتسمعوا الكلام أولها + +427 +00:30:44,220 --> 00:30:50,180 +بيكون صعب أنه أنا أغير سلوكي في مكان معين لكن لما + +428 +00:30:50,180 --> 00:30:55,280 +السلوك أنا أجبره و يصير عادة بيصير بدون تفكير + +429 +00:30:55,280 --> 00:31:00,220 +نفسها بنفسي زي واحد بيفتح الباب و لما يشوف واحد + +430 +00:31:00,220 --> 00:31:07,560 +وراه تمام بنتظر عشان مايسكش الباب في وجهههذه سلوك، + +431 +00:31:07,560 --> 00:31:09,960 +أنا مش مولود في غزل بالمناسبة يعني، لو بلطش في + +432 +00:31:09,960 --> 00:31:15,040 +وجهه عالي مافيش مشكلة، بس برا سلوك، فتلقائي بتلاقي + +433 +00:31:15,040 --> 00:31:18,540 +الواحد يفتح الباب إذا لمح أو شعر إنه حد وراه بضل + +434 +00:31:18,540 --> 00:31:23,700 +وجهه لما يتانى يمسك الباب ويدخل إلى الداخل، بطلت، + +435 +00:31:23,700 --> 00:31:27,520 +بدهاش تفكير، بدهاش جهد، وإنما صارت جزء من مين؟ من + +436 +00:31:27,520 --> 00:31:31,100 +الثقافة، زي ما أنا بالضبط قلتلكوا كيف أنا بشتغل + +437 +00:31:31,100 --> 00:31:36,470 +أنبوبتين وبانقل منهم ال .. البكتيريااللي همبوبتان + +438 +00:31:36,470 --> 00:31:40,230 +بده امسك غطه الأولى و غطه التانية و امررهم على + +439 +00:31:40,230 --> 00:31:43,870 +اللهب تمام و امسك واحدة و أحط في التانية و أرجع + +440 +00:31:43,870 --> 00:31:46,830 +امررهم على اللهب و أسكر الأولى بالأولى و التانية + +441 +00:31:46,830 --> 00:31:51,130 +بالتانية طبعا لو قعدنا نشرحها ربع ساعة و ندربكوا + +442 +00:31:51,130 --> 00:31:54,670 +عليها ربع ساعة هتتغلبوا انا متأكد و هتمارسوا + +443 +00:31:54,670 --> 00:31:58,990 +هتتغلبوا لكن لو مارستوها مرة و مرتين و تلاتة و + +444 +00:31:58,990 --> 00:32:03,180 +أربعهتلاقوا ان الامر صار سهل فانا بقول استغلوا + +445 +00:32:03,180 --> 00:32:05,920 +فرصة وجودكم في مختبر ال microbiology لتعديل السلوك + +446 +00:32:05,920 --> 00:32:11,160 +لتعديل السلوك بحيث انه إذا لبسنا gloves طبعا ال + +447 +00:32:11,160 --> 00:32:16,080 +gloves هذه is not to protect yourself only but to + +448 +00:32:16,080 --> 00:32:19,640 +protect others and the environment إذا مسكت أي + +449 +00:32:19,640 --> 00:32:23,360 +أداء حتى لو مش إلك في المختبر طبعا و أنت مسكة ال + +450 +00:32:23,360 --> 00:32:28,250 +glovesانت حطيت الميكروبات اللى المفروض تحميل جسمك + +451 +00:32:28,250 --> 00:32:31,990 +يكون محبي منها حطيتها على هذه الأداة صح؟ يعني + +452 +00:32:31,990 --> 00:32:34,410 +بجمعنا الحاكي انا مش ان انا لبست ال gloves يعني + +453 +00:32:34,410 --> 00:32:39,190 +انا protected و الأخرين protected لأ بالعكس اذا + +454 +00:32:39,190 --> 00:32:44,390 +لبست ال gloves و اتصرفت بسلوك aseptic technique + +455 +00:32:44,390 --> 00:32:47,910 +نعم هيك انا بكون اشتغلت صح ب .. بس اذا لبست gloves + +456 +00:32:47,910 --> 00:32:51,850 +و انا بتعطيكوا هو بس يعني خالي منطق الموضوع لو + +457 +00:32:51,850 --> 00:32:56,040 +حطينا على أكرة الباب اللى برا هنا مادة مشعةو + +458 +00:32:56,040 --> 00:32:59,840 +جناكوا تفضلوا على الصفة ماحدش شافها المادة اللي + +459 +00:32:59,840 --> 00:33:02,780 +شافها ولا حد بيعرفها منكوا كلكوا خدتوها و خدتوها + +460 +00:33:02,780 --> 00:33:08,480 +على يدكوا و دخلتوا الصفة طبعا الان طفلنا رضواه و + +461 +00:33:08,480 --> 00:33:12,580 +شغلنا إضاءة باللواء المعينة بتكشف المادة المشعة + +462 +00:33:15,070 --> 00:33:18,270 +وشفناكوا وشفنا وين المادة المشعة حتي لايجيها + +463 +00:33:18,270 --> 00:33:22,910 +الجرابين تحت، بالشعر هانا، حسب وين الواحد حط إيده + +464 +00:33:22,910 --> 00:33:27,510 +اللي لمسها، صح؟ هتستغرب وين موجودة، حتدور في الشرط + +465 +00:33:27,510 --> 00:33:32,110 +بعتكوا، في الدفاتر، عن نضرات، في أماكن مختلفة و + +466 +00:33:32,110 --> 00:33:36,750 +متنوعة من أجسامكوا، تمام؟ موجودة، المادة المشعة، + +467 +00:33:36,750 --> 00:33:42,170 +وهذا الكلام بيعملوه كاختبار لتوظيف بعض الأحيان في + +468 +00:33:42,170 --> 00:33:43,110 +بعض المؤسسات الكبيرة + +469 +00:33:46,440 --> 00:33:50,560 +بيدخلوا المرشحين الوظيفة عبر الباب ال hand اللي + +470 +00:33:50,560 --> 00:33:54,020 +تبعتوا فيها المادة المشعة وبعد ساعة بعطواهم تجربة + +471 +00:33:54,020 --> 00:33:59,840 +معينة كويسة اشتغلوها وبعد ساعة اقولهم stop اطفضواه + +472 +00:33:59,840 --> 00:34:05,080 +ادوضواه شوفوا ان اقلهم تلوثة ان هم اللي بياخدوه و + +473 +00:34:05,080 --> 00:34:08,760 +بيوصلوا اقلهم تلوثة بس يكون ده عمل اشتغل مش اقلهم + +474 +00:34:08,760 --> 00:34:11,320 +تلوثة يعني اقلهم تلوثة ان هم اشتغلوا يكون اعلى + +475 +00:34:11,320 --> 00:34:17,040 +تجربة برضه كمان القصد من الكلامإنه ال aseptic + +476 +00:34:17,040 --> 00:34:19,660 +technique و ال hand washing مهم جدا في أي عمل في + +477 +00:34:19,660 --> 00:34:23,480 +البيت في ال .. في ال .. في المطبخ في الجامعة في + +478 +00:34:23,480 --> 00:34:27,520 +المكتبة لكن له أهمية أكبر فيه و خمساش عشر من كل + +479 +00:34:27,520 --> 00:34:31,020 +عام اسمه hand washing day يعني لا تغسلوش إيدكوا + +480 +00:34:31,020 --> 00:34:36,140 +إلا في خمساش عشر من كل سنةهو يوم غسيل لدين + +481 +00:34:36,140 --> 00:34:39,060 +فاستظلوا لهذا اليوم و بصيروا جاجوا ان شاء الله + +482 +00:34:39,060 --> 00:34:45,180 +طبعا هذا اليوم خصص عشان خاطر تثقيف الناس و العالم + +483 +00:34:45,180 --> 00:34:49,380 +بأهمية غسل لدين و انا بقولكوا هي حقيقة علمية و + +484 +00:34:49,380 --> 00:34:52,480 +ليست تسوقية 80% من ال infectious diseases could be + +485 +00:34:52,480 --> 00:34:56,740 +prevented just by hand washing 80% of infectious + +486 +00:34:56,740 --> 00:35:00,000 +diseases could be prevented just by hand .. مش + +487 +00:35:00,000 --> 00:35:02,420 +عفوا by hand washing .. by proper hand washing + +488 +00:35:02,930 --> 00:35:05,310 +Rubber hand washing اللي هو قس اليدين بطريقة + +489 +00:35:05,310 --> 00:35:11,690 +الصحيحة طيب ال infective dose بتاعة البكتيريا + +490 +00:35:11,690 --> 00:35:14,630 +اتراوح من عشرة إلى متام حتى وجوا في كتب من واحد + +491 +00:35:14,630 --> 00:35:18,530 +لمية من واحد لخمسة يعني مختلفة لكن في الآخر هي عدد + +492 +00:35:18,530 --> 00:35:24,130 +قليل وال ID50 إذا فاكرين هقولنا هي مقياس لل + +493 +00:35:24,130 --> 00:35:28,170 +virulence مثلا بال term fresh ال virulence ال + +494 +00:35:28,170 --> 00:35:31,930 +virulence كل ما قل ال ID50 كل ما عليه ال virulence + +495 +00:35:32,930 --> 00:35:38,530 +قررنا الشجلة بال Vibrio واحد او عشرين لمتان و + +496 +00:35:38,530 --> 00:35:44,410 +بالمقارنة مع مليون خلية يبقى العشرة او العشرين + +497 +00:35:44,410 --> 00:35:50,640 +لمتان more virulent من اللي هو المليونThere is an + +498 +00:35:50,640 --> 00:35:52,700 +incubation period of one to seven days فكده قبل + +499 +00:35:52,700 --> 00:35:54,340 +شويه هقولنا ان ال incubation period من اتنين لتلت + +500 +00:35:54,340 --> 00:35:56,860 +أيام هذه ال average أو ال most common لكن في شكل + +501 +00:35:56,860 --> 00:36:00,740 +عام ممكن تستمر مع بعض الناس على سبع أيام Fallot by + +502 +00:36:00,740 --> 00:36:04,580 +fever, cramping, مغص, abdominal pain, ألم في + +503 +00:36:04,580 --> 00:36:09,600 +المعدة, water diarrhea due to the toxin هذا + +504 +00:36:09,600 --> 00:36:12,680 +العملية بتستمر من واحد لتلت أيام اللي هو الإسهال + +505 +00:36:12,680 --> 00:36:17,530 +الشريد هذابعد تلت أيام غالبا ال .. ال .. ال .. ال + +506 +00:36:17,530 --> 00:36:17,710 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +507 +00:36:17,710 --> 00:36:17,870 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +508 +00:36:17,870 --> 00:36:18,270 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +509 +00:36:18,270 --> 00:36:18,730 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +510 +00:36:18,730 --> 00:36:20,730 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +511 +00:36:20,730 --> 00:36:27,890 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +512 +00:36:27,890 --> 00:36:29,030 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +513 +00:36:29,030 --> 00:36:29,870 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +514 +00:36:29,870 --> 00:36:29,870 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +515 +00:36:29,870 --> 00:36:30,050 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +516 +00:36:30,050 --> 00:36:30,050 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +517 +00:36:30,050 --> 00:36:32,950 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +518 +00:36:32,950 --> 00:36:36,330 +.. ال + +519 +00:36:40,900 --> 00:36:43,720 +it is rare for the organism to disseminate هذا + +520 +00:36:43,720 --> 00:36:47,600 +الجبل حاجناها، الميكروب غالبا لا يُعزل إلا من وين + +521 +00:36:47,600 --> 00:36:51,460 +من الـintestinal tract the severity of the disease + +522 +00:36:51,460 --> 00:36:54,940 +is dependent on the strain يعني بعتمد على أفعا on + +523 +00:36:54,940 --> 00:36:59,560 +the species أكترهم شراسة وأكترهم قدروا على إحداث + +524 +00:36:59,560 --> 00:37:03,600 +severity في الشجلة اللي هو Shigella Dysentery is + +525 +00:37:03,600 --> 00:37:06,840 +the most followed by Flexnery, Sunni and Boy + +526 +00:37:09,440 --> 00:37:16,240 +ال immunity عشان SIGA هذا soluble immunoglobulin A + +527 +00:37:16,240 --> 00:37:20,700 +أو سيكراتوري اللي هنقول عنه هو بيكون وامو .. + +528 +00:37:20,700 --> 00:37:23,180 +السيكراتوري هذا بيكون على ال mucus membrane على ال + +529 +00:37:23,180 --> 00:37:28,510 +mucus membrane لكن برضه ال immunityمش هال .. يعني + +530 +00:37:28,510 --> 00:37:31,830 +لو الإنسان قصيب اليوم شيجيلوسس اللي من الاتباع .. + +531 +00:37:31,830 --> 00:37:35,470 +اللي من الاتباعته may not last for six months حتى + +532 +00:37:35,470 --> 00:37:39,990 +ست شهورها بتكملش، لو .. لو شرب مرة تانية شجيلة + +533 +00:37:39,990 --> 00:37:45,210 +ممكن تعمله infection ال diagnosis لل .. للشجيلة + +534 +00:37:45,210 --> 00:37:49,750 +infection غالبا بالستول، stool culture، يعني بناخد + +535 +00:37:49,750 --> 00:37:53,740 +الدياريةإذا كان المريض مصل لمرحلة إنه مافيش stool + +536 +00:37:53,740 --> 00:37:57,000 +بنستخدم ما يسمى بال rectal swab يبقى عندي يا إما + +537 +00:37:57,000 --> 00:38:00,040 +stool و swab يعني swab بناخده في ال rectum و + +538 +00:38:00,040 --> 00:38:02,800 +بنعمله rolling بناخد ال whatsoever المتيرة + +539 +00:38:02,800 --> 00:38:05,760 +الموجودة أكيد هتكون محتوى على مين؟ على ال micro + +540 +00:38:05,760 --> 00:38:09,640 +organism بس دايما ال stool عينة مفضلة عن اللي هو + +541 +00:38:09,640 --> 00:38:12,700 +ال rectal swab بس في حالة أدم وجود stool بناخد + +542 +00:38:12,700 --> 00:38:17,160 +اللي هو rectal swabطبعا العملية بتتم في .. في .. + +543 +00:38:17,160 --> 00:38:20,940 +من خلال مزرعة وعملت identification ممكن استخدم + +544 +00:38:20,940 --> 00:38:23,000 +quick immunological method إيش يعني quick + +545 +00:38:23,000 --> 00:38:26,300 +immunological method؟ الستول أنا قولتلكوا دايما في + +546 +00:38:26,300 --> 00:38:30,920 +الأوبئة بنلجأ إلى إنه نقصر فترة ال diagnosis قدر + +547 +00:38:30,920 --> 00:38:34,580 +المستطاع، مظبوط و مش مظبوط، بمعنى لو احنا في ال .. + +548 +00:38:34,580 --> 00:38:37,120 +ال .. ال .. المزرعة تاخد معنا 72 ساعة، بدنا نحاول + +549 +00:38:37,120 --> 00:38:40,140 +قدر الإمكان خلال 24 ساعة، وإذا أقل خلال نص ساعة، + +550 +00:38:40,140 --> 00:38:44,900 +لماذا .. لماذا .. لماذا أتأخر أكتر من نص ساعة؟فلو + +551 +00:38:44,900 --> 00:38:47,960 +أخدنا عينة ستول و عملنا لها fixation على شريحة و + +552 +00:38:47,960 --> 00:38:50,560 +استخدمنا fluorescent antibody ايش يعني fluorescent + +553 +00:38:50,560 --> 00:38:54,860 +antibody؟ يعني antibody ضد الشجلة بس labelled + +554 +00:38:54,860 --> 00:38:59,300 +بـfluorescent و أعطينا فرصة لل antibody اللي هو ال + +555 +00:38:59,300 --> 00:39:01,700 +fluorescent labelled انه يتفاعل مع البكتيريا اللي + +556 +00:39:01,700 --> 00:39:05,620 +موجهة بالبراز مليان بكتيريا من الشجلة و غير الشجلة + +557 +00:39:05,620 --> 00:39:11,140 +إذا الشجلة موجودة هيرتبط فيها ال antibody بنغسل + +558 +00:39:11,140 --> 00:39:16,420 +الشريحةبنغسل الشريحة خدوا بالكم البكتيريا fixed + +559 +00:39:16,420 --> 00:39:21,520 +صح؟ على الشريحة الان لو ال antibody اللي احنا + +560 +00:39:21,520 --> 00:39:25,760 +حطناه التسخ بالبكتيريا هيصيب برضه fixed مع + +561 +00:39:25,760 --> 00:39:29,740 +البكتيريا اللي هي fixed على الشريحة فلو غسلنا مش + +562 +00:39:29,740 --> 00:39:35,200 +هيروح من البكتيريا لكن لو الشيقلة مش موجودة ال + +563 +00:39:35,200 --> 00:39:39,120 +antibody بيرتبط مع مين؟ ولا اشي فلما نعمل غسيل + +564 +00:39:39,120 --> 00:39:43,420 +بيروح مع الغسيلبعد ما بنجفف الشريحة بنحطها تحت ال + +565 +00:39:43,420 --> 00:39:47,240 +fluorescent microscope UV بتطلب .. ت .. تضرب + +566 +00:39:47,240 --> 00:39:51,060 +الشريحة إذا في fluorescent .. مادة fluorescent + +567 +00:39:51,060 --> 00:39:55,300 +تمضص ال UV و بتشير لنا visible light إذا أنا شفت + +568 +00:39:55,300 --> 00:39:59,040 +الآن إيش؟ visible light اللون المعينة حسب اللي هو + +569 +00:39:59,040 --> 00:40:02,940 +اللون اللي أنا بستخدمه في مين في ال antibody .. ال + +570 +00:40:02,940 --> 00:40:06,120 +fluorescent antibodyإذا شوفت light معناه لحاجة ال + +571 +00:40:06,120 --> 00:40:08,760 +fluorescent موجود ليش ال fluorescent موجود؟ لأن ال + +572 +00:40:08,760 --> 00:40:12,320 +antibody موجود ليش ال antibody موجود لحد الآن بدل + +573 +00:40:12,320 --> 00:40:15,860 +غصير؟ لأن البكتيريا موجودة خلال نصف ساعة نقدر نعرف + +574 +00:40:15,860 --> 00:40:19,480 +النتيجة انه positive ولا negative لكن لا تبني عن + +575 +00:40:19,480 --> 00:40:25,300 +ال culture، ليش؟ لأنه بتعمل كمان sensitivity بتشوف + +576 +00:40:25,300 --> 00:40:29,920 +مدى حساسية البكتيريا لمين؟ ل .. اللي هو ال .. ال + +577 +00:40:29,920 --> 00:40:31,760 +.. ال .. ال .. ال .. ال antibiotics اللي هنقوله + +578 +00:40:31,760 --> 00:40:34,830 +قبل شوية في اللوحات السادقةإن البكتيريا مشهورة + +579 +00:40:34,830 --> 00:40:40,750 +عنها إنها بتكون resistance Prevention فيه vaccine + +580 +00:40:40,750 --> 00:40:44,870 +بستخدمه في المصدر الموضوع Life attenuated shigella + +581 +00:40:44,870 --> 00:40:48,690 +dysenteria vaccine إيش يعني life attenuated يعني + +582 +00:40:48,690 --> 00:40:57,610 +البكتيريا حية بنعملها بمعاملة معينة بنقلل من ال + +583 +00:40:57,610 --> 00:41:01,320 +violence تبعها بنقلل من ال violence تبعهافبتعمل + +584 +00:41:01,320 --> 00:41:07,300 +mild infection انفكشن بسيطة الجهاز المناع بتغلب + +585 +00:41:07,300 --> 00:41:11,220 +عليها لكن كمان مرة ال immunity تبعتها short + +586 +00:41:11,220 --> 00:41:16,860 +فبالتالي بتعطى للناس مثلا الدكتور بدروح على يتطوع + +587 +00:41:16,860 --> 00:41:21,520 +في منطقة فيها و بقلة الشجرة قبل ما يروح بيعطوب هذا + +588 +00:41:21,520 --> 00:41:24,540 +ال vaccine عشان هو يحمي نفسه خلال فترة وجوده في + +589 +00:41:24,540 --> 00:41:29,750 +هذه المنطقة كمان مرة بأكدلكوا على هميةالـ Safe + +590 +00:41:29,750 --> 00:41:39,070 +Water في الحروب، في الكوارث، في الأمطار، في + +591 +00:41:39,070 --> 00:41:43,630 +الزلازل، في الهجرات اللي بتصير، يجب أن يقتنع .. + +592 +00:41:43,630 --> 00:41:47,290 +لذلك أنا بقول إن من ثقافة الأفراد على مستوى العالم + +593 +00:41:47,290 --> 00:41:51,250 +يجب أن يكون كيف يتحصل على Safe Water في معظم + +594 +00:41:51,250 --> 00:41:56,490 +الأحواليعني .. يعني قدر الميه استطيع لو بديه + +595 +00:41:56,490 --> 00:42:01,190 +يستخدم جميص ويفلتر الميه يعني أقل .. يعني .. يعني + +596 +00:42:01,190 --> 00:42:05,190 +أحسن من إنه ميفلترهاش لو عنده إمكانية تسخينها أو + +597 +00:42:05,190 --> 00:42:08,950 +غاليها بكون أفضل لو عنده إمكانية إضافة بعض قطلات + +598 +00:42:08,950 --> 00:42:13,010 +من ال chlorine على الميه برضه بكون كويس تعرف + +599 +00:42:13,010 --> 00:42:19,310 +الشاب؟الألم .. الشبه هذه .. تمام؟ هذه بيستخدموها + +600 +00:42:19,310 --> 00:42:23,450 +عالميا في عملية تنقية .. تنقية المياه بإنه ت .. ت + +601 +00:42:23,450 --> 00:42:27,590 +.. ت .. تنطحن بشكل بودرة و لو كانت المياه يعني + +602 +00:42:27,590 --> 00:42:32,830 +مليئة بالشوائب و حاطين عليها الشبه بعد فترة + +603 +00:42:32,830 --> 00:42:37,910 +بترصبها تمام؟ بترصب و بناخد احنا السائل المظيف و + +604 +00:42:37,910 --> 00:42:43,600 +بنفلتره كمرة عبر .. عبر قطعة كماشةلو كانت الشباب + +605 +00:42:43,600 --> 00:42:47,620 +المتوفرة وكان الكلور متوفر حلت مشكلة الميه، مهما + +606 +00:42:47,620 --> 00:42:51,120 +كانت نوع الميه، تمام؟ غالبا بيقدر أنه نحصل على + +607 +00:42:51,120 --> 00:42:56,620 +مياه آمنة، لكن الغالي دايما بيكون برضه وسيلة جيدة + +608 +00:42:56,620 --> 00:42:59,820 +Treating scoliosis, manage dehydration, manage + +609 +00:42:59,820 --> 00:43:02,940 +electrolytes, patients respond to antibiotics، فيه + +610 +00:43:02,940 --> 00:43:06,900 +أنها مشكلة بكلمات صغيرة problem of drug resistance + +611 +00:43:07,800 --> 00:43:11,380 +أذكركوا بالديارية بشكل عام لتريتمنت تبعتها كانت + +612 +00:43:11,380 --> 00:43:17,820 +تتلخص في تلت شغلات رئيسي water replacement، + +613 +00:43:17,820 --> 00:43:20,440 +electrolyte replacement، هدول التانية اللي حطيناهم + +614 +00:43:20,440 --> 00:43:23,660 +في معلوق محلول معالجة الجفاف، التانية هم بيعطيه + +615 +00:43:23,660 --> 00:43:25,260 +ميه و بيعطيه محلول في نفس ال electrolyte في نفس + +616 +00:43:25,260 --> 00:43:30,820 +الوقت، تمام؟ التالتة اللي هو ال eradication of the + +617 +00:43:30,820 --> 00:43:33,430 +causative agentماذا يعني eradication of the cause + +618 +00:43:33,430 --> 00:43:35,910 +of the pain؟ إذا العامل المسبب إنت إيه في + +619 +00:43:35,910 --> 00:43:39,990 +هالستولاتكا، بتدعي المريض antiprotozoa زي + +620 +00:43:39,990 --> 00:43:44,630 +الفلاجيل، زي الفلاجيل إذا كان سالمونيلا أو شجلة، + +621 +00:43:44,630 --> 00:43:48,670 +بتدعي أنت بيتك مناسب لقتل السالمونيلا أو الشجلة، + +622 +00:43:48,670 --> 00:43:52,690 +هذا معناه eradication، إذن هي تلت نقاط رئيسية + +623 +00:43:52,690 --> 00:43:56,290 +water، electrolyte replacement، بعدها eradication + +624 +00:43:56,290 --> 00:44:02,900 +تعتمد على مين المسبب؟ مين المسبب؟المرة الجاية ان + +625 +00:44:02,900 --> 00:44:06,700 +شاء الله بننتقل لموضوع آخر ليا سلمونيلا و بننهي ان + +626 +00:44:06,700 --> 00:44:07,220 +شاء الله هذا + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/b5cF6_LhnGA_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/b5cF6_LhnGA_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..8a8ef059a350194e9df80db0b803f0295617bbc7 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/b5cF6_LhnGA_postprocess.srt @@ -0,0 +1,2312 @@ +1 +00:00:21,670 --> 00:00:24,070 +بسم الله الرحمن الرحيم اليوم موضوع جديد ان شاء + +2 +00:00:24,070 --> 00:00:28,450 +الله bacterial genetics موضوع ممتع جدا و فيه + +3 +00:00:28,450 --> 00:00:32,570 +معلومات قد تساعدنا في فهم كثير من الأمور و + +4 +00:00:32,570 --> 00:00:36,410 +المعلومات أيضا لها تطبيقات جيدة في مجال ال + +5 +00:00:36,410 --> 00:00:42,580 +clinical and medical microbiomeفده هو الفصل الرابع + +6 +00:00:42,580 --> 00:00:46,580 +ان شاء الله bacterial genetics نذكركم بس بإيش + +7 +00:00:46,580 --> 00:00:49,900 +المحتوى الوراثي للبكتيريا، يعني البكتيريا إيش فيها + +8 +00:00:49,900 --> 00:00:53,840 +نواد وراثية؟ لجينا فيها chromosome وهو single، + +9 +00:00:53,840 --> 00:00:58,800 +ضعيف لعب، ولكن circular، بعض البكتيريا اللي لجينا + +10 +00:00:58,800 --> 00:01:04,290 +فيها plasmidsبالظبط ال extra chromosomal elements + +11 +00:01:04,290 --> 00:01:07,910 +اللي هي سمنها self-replicating و it replicates + +12 +00:01:07,910 --> 00:01:10,570 +independently و كلمة independently في التعريف اللي + +13 +00:01:10,570 --> 00:01:14,390 +قبلنا مكتوبة بشكل autonomous autonomous يعني ذاتي + +14 +00:01:14,390 --> 00:01:18,410 +يعني فيها استقلالية فيها استقلالية المصطلح epizome + +15 +00:01:18,410 --> 00:01:23,530 +مر معانا بس ممرش بهذا المسمة فاكرين لما قلنالكوا + +16 +00:01:23,530 --> 00:01:28,130 +أن ال plasma قد ترتبط بالكرومزوم و أن الكرومزوم + +17 +00:01:28,130 --> 00:01:33,390 +ممكن ينتقل كله إلى بكتير أخرىالبلازميد اللى بترتبط + +18 +00:01:33,390 --> 00:01:37,450 +بال chromosome اسمها ابيزوم يعني الابيزوم عبارة عن + +19 +00:01:37,450 --> 00:01:40,850 +ايش؟ عبارة عن بلازميد متطابطة بيه اللى هو نعم + +20 +00:01:40,850 --> 00:01:43,430 +integrated في ال bacterial chromosome بال + +21 +00:01:43,430 --> 00:01:49,050 +chromosome لو + +22 +00:01:49,050 --> 00:01:55,810 +حد أمامكوا بتوضحلنا نقطة مهمة جدا جدا اسم فطر اسم + +23 +00:01:55,810 --> 00:02:00,660 +واحدخمسة mutation خمسة mutation طلعوا على الصورة + +24 +00:02:00,660 --> 00:02:04,360 +أو على الصورة هتلاقوا أنه ألوانهم الخمسة مختلفة + +25 +00:02:04,360 --> 00:02:09,400 +على الرغم من أنهم نفس الإسم اللي حدث فيهم mutation + +26 +00:02:09,400 --> 00:02:14,030 +طفيف يعني صار فيه تغيير في المادة الوراقيةوبالتالي + +27 +00:02:14,030 --> 00:02:19,390 +بدلاش نهمل أي تغيير بيحدث في البكتيريا أو + +28 +00:02:19,390 --> 00:02:23,630 +الفيروسات لأن أي تغيير طفيف في ال genotype قد يؤدي + +29 +00:02:23,630 --> 00:02:27,710 +إلى تغير في ال phenotype والأن واجب علينا نوضح مع + +30 +00:02:27,710 --> 00:02:33,570 +معناه genotype و phenotype بسأل سؤال .. سؤال + +31 +00:02:33,570 --> 00:02:38,670 +افتراضي لو أن هناك مخلوقين متطابقين في المادة + +32 +00:02:38,670 --> 00:02:43,990 +الوراثيةمتطابقين زي بكتيريا انقسمت، بكتيريا انقسمت + +33 +00:02:43,990 --> 00:02:49,650 +فهي متطابقة، هذا النسخة نفس النسخة، هل هناك شرط ان + +34 +00:02:49,650 --> 00:02:53,470 +يكونوا ال phenotype نفس الشكل، يعني الملاخق + +35 +00:02:53,470 --> 00:02:58,530 +بيحملوا نفس الصفات الخارجية؟ لأ، ليش لأ؟ مدام + +36 +00:02:58,530 --> 00:03:02,650 +المادة الوراثية واحدة، لازم يكونوا نفس الشيء، و لا + +37 +00:03:02,650 --> 00:03:03,530 +لأ؟ + +38 +00:03:06,150 --> 00:03:11,630 +لأنه أعطيكم إثارة بسيطة جدا من أنفسكم، الآن أنتوا + +39 +00:03:11,630 --> 00:03:15,850 +عندكم خلايا في العين و خلايا في الجلد و خلايا في + +40 +00:03:15,850 --> 00:03:19,770 +الكبد و خلايا في القلب، كلهم بيحملوا نفس المادة + +41 +00:03:19,770 --> 00:03:24,730 +الوراتية، لكن هذه خلية قلب cardiacو هذه خلية nerve + +42 +00:03:24,730 --> 00:03:29,110 +و هذه خلية eye و هذه خلية skin بيختلفوا تماما عن + +43 +00:03:29,110 --> 00:03:32,750 +بعضهم البعض ما هو سبب الاختلاف أنه في عندي switch + +44 +00:03:32,750 --> 00:03:36,130 +on و switch off لبعض الجينات يعني جينات مطفقة + +45 +00:03:36,130 --> 00:03:42,510 +وجينات مشغلة أعطيكم مثال تاني أوضح لو أنا عندي + +46 +00:03:42,510 --> 00:03:47,210 +أربع أو خمس أنواع من الإضاءات يعني ضوء أحمر أخضر + +47 +00:03:47,210 --> 00:03:52,770 +أزرق أصفر نفس النسخة اللي معايا معاكم أنتمهل + +48 +00:03:52,770 --> 00:03:56,910 +بالضرورة نحصل على نفس الإضاءة مع بعضنا؟ طب لو أنا + +49 +00:03:56,910 --> 00:04:01,370 +طفيت اللامبة الزرقية و أنتوا خلتوا اللامبات كلهم + +50 +00:04:01,370 --> 00:04:06,510 +ضوين يبقى ليس بالضرورة تشابه ال genotype أن يؤدي + +51 +00:04:06,510 --> 00:04:13,110 +إلى تشابه ال phenotype قد يبدو .. قد يبدو ال + +52 +00:04:13,110 --> 00:04:16,630 +genotype متطابق أو قد .. قد يكون متطابق لكن ال + +53 +00:04:16,630 --> 00:04:22,600 +phenotype مرة مختلفة طيباللي خلانا نتكلم عن هذا + +54 +00:04:22,600 --> 00:04:26,640 +الموضوع ان احنا بنقول ان البكتيريا بتتغير باستمرار + +55 +00:04:26,640 --> 00:04:31,100 +و المحاضرة السابقة لما تحدثنا عن تغيير الأسماء لما + +56 +00:04:31,100 --> 00:04:33,900 +قولنا ان البكتيريا بتسمى بال Binomial System Genes + +57 +00:04:33,900 --> 00:04:37,620 +و Species و جيبنا بعض الأمثلة قولنا تغيرت أسماءها + +58 +00:04:37,620 --> 00:04:40,320 +ياسينيابيستس كان اسمها Pastoreliopistis + +59 +00:04:40,320 --> 00:04:45,700 +انتروباكترساكزاكي كان اسمها كرونوباكترساكزاكي طيب + +60 +00:04:45,700 --> 00:04:49,520 +هذا التغيير احنا عزوناه المحاضرة الماضية لأن احنا + +61 +00:04:49,520 --> 00:04:54,270 +بنعرفشتعلمنا جديدًا صنعنا تقنيات جديدة عرفنا + +62 +00:04:54,270 --> 00:04:57,190 +معلومات جديدة عن البكتيريا فغيرناها طب ما بالك أن + +63 +00:04:57,190 --> 00:05:01,550 +البكتيريا ممكن تتغير أصلا من صفتها؟ وهذا حقيقة و + +64 +00:05:01,550 --> 00:05:05,730 +واقع أن البكتيريا ممكن تتغير من صفتها وأسهل مثال + +65 +00:05:05,730 --> 00:05:09,510 +.. يعني خلّيني أقول أوضح مثال بكتيريا بقت + +66 +00:05:09,510 --> 00:05:14,100 +sensitive للبنسالين صارت resistant للبنسالينهل ليس + +67 +00:05:14,100 --> 00:05:17,740 +هذا تغير في الصفات؟ بكتيريا لما تكون ممرضة و أصبحت + +68 +00:05:17,740 --> 00:05:21,980 +ممرضة، هل ليس هذا تغير في الصفات؟ إذا ما الذي يغير + +69 +00:05:21,980 --> 00:05:27,100 +الصفات الظاهرية هو تغيير جيني، تغيير الجيني، الآن + +70 +00:05:27,100 --> 00:05:30,480 +كيف للبكتيريا أن تتغير جينيا؟ طلعوا اللوحة اللي + +71 +00:05:30,480 --> 00:05:36,400 +قدامكم، في عندي gene mutation، طفرات، و الطفرات + +72 +00:05:36,400 --> 00:05:41,790 +نوعانان هو spontaneous بمعنى انه تلقاء من تلقاء + +73 +00:05:41,790 --> 00:05:45,970 +ذات البكتيريا بتعمل ال mutation و هتقولك رقم و بدك + +74 +00:05:45,970 --> 00:05:51,390 +تقولولي الرقم هذا كبير ولا صغير فرصة حدوث mutation + +75 +00:05:51,390 --> 00:05:57,510 +تلقائية في البكتيريا واحد في كل مائة مليون قليل + +76 +00:05:57,510 --> 00:06:03,430 +كرقملكن لو تخيلنا أن البكتيريا .. الخلية الواحدة + +77 +00:06:03,430 --> 00:06:07,330 +.. الخلية الواحدة قد تنقسم إلى مائة مليون أصلا في + +78 +00:06:07,330 --> 00:06:10,450 +يوم واحد فبالتالي .. فمعناته كتير أما لو قولنا + +79 +00:06:10,450 --> 00:06:15,810 +الرقم هذا في الإنسان .. بيقول عنه قليل لإن صورة + +80 +00:06:15,810 --> 00:06:18,890 +الانتقالات أن الإنسان بطيئة جدا بالمقارنة مع مين؟ + +81 +00:06:18,890 --> 00:06:22,870 +مع البكتيريا إذن الرقم اللي ذكرته إن كان يبدو قليل + +82 +00:06:22,870 --> 00:06:27,390 +لكن هو رقم ضخم جدا جدا جدا في البكتيريا وصلت صورة؟ + +83 +00:06:27,390 --> 00:06:31,780 +طيبالـ spontaneous mutation بيعزوها العلماء إلى + +84 +00:06:31,780 --> 00:06:36,800 +أخطاء في عملية الـ replication أساسا يعني أنتوا + +85 +00:06:36,800 --> 00:06:41,080 +عارفين ال .. ال .. ال chromosome عشان ينقسم أو + +86 +00:06:41,080 --> 00:06:45,280 +يصير ال DNA replication بدنا بعض ال enzymes منها و + +87 +00:06:45,280 --> 00:06:47,540 +نفطر ال .. ال .. ال DNA polymerase ال DNA + +88 +00:06:47,540 --> 00:06:51,890 +polymeraseهناك نسبة خطأ لل DNA polymerase نسبة خطأ + +89 +00:06:51,890 --> 00:06:55,230 +لل DNA polymerase وبالتالي بيحدث أخطاء وبالتالي + +90 +00:06:55,230 --> 00:06:59,390 +بيصير لنا ال mutation سميناها spontaneous سميناها + +91 +00:06:59,390 --> 00:07:03,890 +spontaneous لإنه تحدث بدون سبب ماعناش سبب معين + +92 +00:07:03,890 --> 00:07:08,750 +اللي نقول والله صارت ال mutation بسببه عكسها هيكون + +93 +00:07:08,750 --> 00:07:17,030 +إيش induced induced يعني بفعل فاعل كلكم بتعرفوا إن + +94 +00:07:17,030 --> 00:07:22,590 +ال ultraviolet lightال UV قد تسبب skin cancer اللي + +95 +00:07:22,590 --> 00:07:26,050 +هان تتكلم عن إيه دخل ال cancer و ال mutation كل .. + +96 +00:07:26,050 --> 00:07:31,990 +كل cancer أساسه mutation مش بالضرورة اخدوا بالكوا + +97 +00:07:31,990 --> 00:07:36,050 +ان كل mutation تؤدي ل cancer بس كل cancer أساسه + +98 +00:07:36,050 --> 00:07:41,210 +mutation متفقنا على هذا يمكن اتفقنا على ان ال UV + +99 +00:07:41,210 --> 00:07:46,090 +بتحط الخلايا اللي نصل فيها ال mutation و هنعرف هذا + +100 +00:07:46,090 --> 00:07:49,600 +الكلامالموضوع الجاي لما اتكلم عن ال control of + +101 +00:07:49,600 --> 00:07:53,080 +microorganism بستخدم ال UV في قتل البكتيريا بحدها + +102 +00:07:53,080 --> 00:07:59,400 +طبعا يبقى spontaneous و ايش كمان induced طبعا + +103 +00:07:59,400 --> 00:08:02,200 +induced بفعل أشياء كثيرة و خاصة في زماننا اللي + +104 +00:08:02,200 --> 00:08:05,120 +احنا الآن عايشين فيه كتير من المركبات المصنعة + +105 +00:08:05,120 --> 00:08:09,920 +synthetic compounds يعتقد انه و التلوث يعني هناك + +106 +00:08:09,920 --> 00:08:16,050 +مياه أنهار الآنيعني تعتبر المياه تبعتها مطفرة تعمل + +107 +00:08:16,050 --> 00:08:20,310 +induction ل ال mutation الآن النوع التاني من + +108 +00:08:20,310 --> 00:08:25,190 +التغيير الجيني بينتج عن ال gene transfer نقل + +109 +00:08:25,190 --> 00:08:31,370 +الجينات من بكتيريا إلى بكتيريا وابتدت طرق رئيسية + +110 +00:08:31,370 --> 00:08:36,210 +transformation conjugation and transduction نبدأ + +111 +00:08:36,210 --> 00:08:40,980 +بالأولى تخيلوا مع التاني ان دي خلية بكتيريةالخلية + +112 +00:08:40,980 --> 00:08:47,340 +البكتيرية عايشة في بيئة منها ممكن تكون ظروف لخلايا + +113 +00:08:47,340 --> 00:08:50,800 +أخرى غير ملائمة بدأت موت الخلايا الأخرى أو بلاش + +114 +00:08:50,800 --> 00:08:55,780 +خلية بكتيرية أكرمكم الله في ال waste water في + +115 +00:08:55,780 --> 00:09:02,250 +المجاري تخيلوا المجاري إيش فيها مواد ورائطيةمن + +116 +00:09:02,250 --> 00:09:06,990 +نباتات، من حيوانات، من إنسان، من بكتيريا Free DNA + +117 +00:09:06,990 --> 00:09:11,690 +ناتج عن مين؟ عن تحلل المواد الأضياء والخلايا يبقى + +118 +00:09:11,690 --> 00:09:17,030 +في عندي abundance وفرة من ال DNA هناك بعض + +119 +00:09:17,030 --> 00:09:21,510 +البكتيريا عندها المقدرة علي إنها تاخد ال DNA من + +120 +00:09:21,510 --> 00:09:28,550 +المحيط تبعها تسحبه إلى الداخل لما يدخل ال DNAإلى + +121 +00:09:28,550 --> 00:09:33,130 +الخلية بيروح و يربطها بال chromosome تبعها كلمة + +122 +00:09:33,130 --> 00:09:36,430 +ربطها بال chromosome صار عندي مادة وراثية من + +123 +00:09:36,430 --> 00:09:40,250 +مصدرين مختلفين خدوا بالكم فلما نربط مادة وراثية من + +124 +00:09:40,250 --> 00:09:42,590 +مصدرين مختلفين نسميها recombination نسميها + +125 +00:09:42,590 --> 00:09:45,410 +recombination الكلمة اللي موجودة على اللوحة دي لها + +126 +00:09:45,410 --> 00:09:52,510 +recombination إذن لما صار في ارتباط للبكتيريا أو + +127 +00:09:52,510 --> 00:09:56,830 +للمادة الوراثية على البكتيريا ال chromosomeو أصبح + +128 +00:09:56,830 --> 00:10:03,590 +عندي اكتساب لمادة وراثية جديدة قد تكون ذات فائدة، + +129 +00:10:03,590 --> 00:10:06,410 +إيش هي قد تكون ذات فائدة؟ هذه المادة أو القطع اللي + +130 +00:10:06,410 --> 00:10:09,130 +حطيناها في البكتيريا الآن أو اللي دخلت البكتيريا + +131 +00:10:09,130 --> 00:10:15,650 +لحالها قد تنتج أنزيم قادر على تكسير مادة عضوية + +132 +00:10:15,650 --> 00:10:19,710 +البكتيريا لم تكن قادرة قبل ذلك على تكسيرها، فأصبح + +133 +00:10:19,710 --> 00:10:25,070 +لديها الآن إيش؟ تنوع، أصبح لديها الآن تنوعفي عملية + +134 +00:10:25,070 --> 00:10:27,970 +اللي هو ال .. ال metabolism زادت قدرتها على + +135 +00:10:27,970 --> 00:10:31,830 +التغذية طب لما زيدت قدرتها على التغذية على مراكبات + +136 +00:10:31,830 --> 00:10:37,610 +متنوعة أليس هذا ميزة إضافية إلها لل survival في ال + +137 +00:10:37,610 --> 00:10:41,690 +environment؟ مظبوط و مش مظبوط هيكتسبت صفة جديدة + +138 +00:10:41,690 --> 00:10:44,730 +صار تغيير وراتي وماصارش تغيير وراتي؟ صار تغيير إسم + +139 +00:10:44,730 --> 00:10:48,110 +العملية كم مرة؟ transformation تعالوا نعبّلها + +140 +00:10:48,110 --> 00:10:53,480 +بكلمة علمية الآنit is the free take up of DNA from + +141 +00:10:53,480 --> 00:10:58,860 +the surrounding باختصار هو الأخذ الحر أو اكتساب + +142 +00:10:58,860 --> 00:11:05,320 +المادة الوراطية من محيط البكتيريا النوع + +143 +00:11:05,320 --> 00:11:08,560 +التاني من ال gene transfer هو ال conjugation و ال + +144 +00:11:08,560 --> 00:11:13,260 +conjugation مر معانا و شفناه و ال transduction ما + +145 +00:11:13,260 --> 00:11:18,420 +مر معانا دعنا نطلع على ال transduction اواطفر وقت + +146 +00:11:18,420 --> 00:11:25,120 +و نعرضلكمالـ Presentation يا ريت الأخوة يعرضوننا + +147 +00:11:25,120 --> 00:11:34,840 +اللي هو اللي .. اللي بدنا نحطه الآن أمامنا + +148 +00:11:34,840 --> 00:11:38,580 +في اللوحة خلية بكتيرية و مكتوب تحتها donor cell + +149 +00:11:38,580 --> 00:11:43,100 +يعني خلية متبرعة و في وجهها ال bacteriophage ال + +150 +00:11:43,100 --> 00:11:46,480 +bacteriophage هو ال virus اللي بيعمل infection + +151 +00:11:46,480 --> 00:11:51,420 +للبكتيري و خدوا بالكم أحد فوائد هذه المحاضرة + +152 +00:11:51,990 --> 00:11:57,690 +البكتيلوفاج في فائدتين + +153 +00:11:57,690 --> 00:12:01,830 +مهمتين جدا في هذا السياق ان البكتيلوفاج very + +154 +00:12:01,830 --> 00:12:09,170 +specific يعني متخصص مش لسلفي الكوكس اوريس لسلالات + +155 +00:12:09,170 --> 00:12:14,130 +معينة من السلفي الكوكس اوريسيعني زي ما نقول مانفعش + +156 +00:12:14,130 --> 00:12:17,630 +نقول نعمل bacteriophage لبنات سنة تانية في ال .. + +157 +00:12:17,630 --> 00:12:21,210 +كل واحدة منهم إلها bacteriophage تخيلوا لو أنا + +158 +00:12:21,210 --> 00:12:27,690 +عندي مخزن في المختبر، من ال bacteriophage تبقى X و + +159 +00:12:27,690 --> 00:12:32,610 +Y و Z و C و S و N، تمام؟ و قلتني حاجة مجهولة، + +160 +00:12:32,610 --> 00:12:37,390 +بكتير مجهولة، مش أنا بقدرمن خلال ال bacteriophage + +161 +00:12:37,390 --> 00:12:41,290 +اللي عندي أتعرف عليها، ليش أتعرف عليها؟ لأنه لن + +162 +00:12:41,290 --> 00:12:44,330 +يحللها، لن يقتلها ال bacteriophage هذه إلا ال + +163 +00:12:44,330 --> 00:12:47,030 +bacteriophage الخاص بيها، وال bacteriophage الآن + +164 +00:12:47,030 --> 00:12:50,990 +معلوم لديه، إذا المعلوم ارتبط بالمجهول، فأصبح + +165 +00:12:50,990 --> 00:12:55,510 +المجهول لنا معلوم، فهي أول فائدة أنه بنستخدمه في + +166 +00:12:55,510 --> 00:12:59,030 +التعرف على ال bacteriophage في المختبر تحت أنواع + +167 +00:12:59,030 --> 00:13:03,550 +نسميها phage typing، phage اختصارا ل bacteriophage + +168 +00:13:03,550 --> 00:13:09,720 +أو phage typingهي أول فائدة استفدناها من الموضوع، + +169 +00:13:09,720 --> 00:13:13,300 +الفائدة التانية، كونه specific وكونه غير مضر + +170 +00:13:13,300 --> 00:13:17,320 +للإنسان، لو احنا انجرحنا وصار عندنا اتهاب جروح + +171 +00:13:17,320 --> 00:13:22,600 +بستافيلوكوكاس أوريس، ألا يمكن استخدام البكتيروفاج + +172 +00:13:22,600 --> 00:13:28,920 +في علاج، في قتل البكتيريا؟والان هناك نشاط محموم + +173 +00:13:28,920 --> 00:13:33,760 +جدا في العالم لإنتاج bacteriophages للاستخدام في + +174 +00:13:33,760 --> 00:13:37,620 +العلاج، تخيلوا بدل ما نعطي المريض اللي عنده الوز، + +175 +00:13:37,620 --> 00:13:41,300 +نعطيه antibiotic إن خليه تهتم ورشله باكتيروفاجة + +176 +00:13:41,300 --> 00:13:47,070 +على الوز، امام؟ خارجيلو واحد عنده Salmonella في + +177 +00:13:47,070 --> 00:13:50,730 +الأمعاق و أعطيناه Anti Salmonella فاجز يبقى فيه + +178 +00:13:50,730 --> 00:13:54,690 +إنه إمكاني أو potential لاستخدام ال bacteriophage + +179 +00:13:54,690 --> 00:13:57,490 +في من؟ في العلاج إذن واحدة في ال diagnosis + +180 +00:13:57,490 --> 00:14:00,350 +والتانية في العلاج تعالوا نطلع على ال + +181 +00:14:00,350 --> 00:14:02,570 +bacteriophage بالأول طبعا فيه كمان فاج ثالثة + +182 +00:14:02,570 --> 00:14:08,310 +هنتكلم عنها لاحقا لما نعرف أنواع الفاجز هذا الفاجز + +183 +00:14:08,310 --> 00:14:13,400 +اللي احنا شايفينه ع السريةباللحظة انه له tail + +184 +00:14:13,400 --> 00:14:19,160 +fibers اللي هم الأطراف المنحنية هذه عشان يقدر + +185 +00:14:19,160 --> 00:14:22,980 +يرتبط بالخلية فجر ال cell wall structure و ان ال + +186 +00:14:22,980 --> 00:14:27,940 +cell wall structure يوفر يوفر إمكانية ارتباط + +187 +00:14:27,940 --> 00:14:32,720 +البكتيريو فهش يبقى مافيش receptor مافيش ارتباط + +188 +00:14:32,720 --> 00:14:36,660 +وهذا اللي بيقدي لمين؟ ل specificity لازم يكون فيه + +189 +00:14:36,660 --> 00:14:41,140 +receptor للبكتيريو فهشالقادر طبط ال bacteriophage + +190 +00:14:41,140 --> 00:14:43,680 +تعالى نشوف إيش بيعمل، بيعمل injection للمادة + +191 +00:14:43,680 --> 00:14:47,260 +الوراثية ومعبّر عنها باللون الأخضر، الآن بدأ + +192 +00:14:47,260 --> 00:14:52,420 +يتكاثر داخل الخلية وكسّر مين؟ البلازميل، مش + +193 +00:14:52,420 --> 00:14:54,800 +البلازميل، هذا ال chromosome، هذا تعبيره عن ال + +194 +00:14:54,800 --> 00:14:58,960 +chromosome، الآن لما بيجي بيعبي، طبعا عملية + +195 +00:14:58,960 --> 00:15:03,140 +التكاثر في ال bacteriophage هي عملية معقدة، لكن + +196 +00:15:03,140 --> 00:15:07,600 +احنا بنفصلها بثلاث مراحل أو أربع مراحلattachment و + +197 +00:15:07,600 --> 00:15:10,640 +penetration شفتوا انا لما اترتسق بدخل المادة + +198 +00:15:10,640 --> 00:15:14,120 +الوراطية صح؟ هذا بحدثها يعني لو اتطلعوا على عملية + +199 +00:15:14,120 --> 00:15:18,820 +نفس هذه عجاز يعني ضخم دخلت المادة الوراطية + +200 +00:15:18,820 --> 00:15:22,220 +البكتيريا فاش انا بقول ماحدة بدخل البكتيريا بقولهم + +201 +00:15:22,220 --> 00:15:27,640 +انا ال boss انا المسئول I am in charge كل الشغل + +202 +00:15:27,640 --> 00:15:31,140 +بتتوجه لخدمتي انا كل كلان يا انزمات يا ريبوزومات + +203 +00:15:31,140 --> 00:15:36,710 +يا اي حاجة في الخير at my service ببدأوايعملوله + +204 +00:15:36,710 --> 00:15:40,450 +replication و ال transcription و ال translation + +205 +00:15:40,450 --> 00:15:45,570 +فاللي بينتج بيكون منتجات فيروسية هذه بيسموها + +206 +00:15:45,570 --> 00:15:52,980 +العملية كلهاال .. البكتيروفاج ال reproduction لكن + +207 +00:15:52,980 --> 00:15:56,460 +في عملية بعدها اسمها assembly ايش هي assembly؟ + +208 +00:15:56,460 --> 00:16:01,480 +تجميع هو .. هو صنع الرأس الـ eicosahedra اللي + +209 +00:16:01,480 --> 00:16:04,760 +شايفينها زي الصندوق هذه تمام؟ صنع ال tail fibers + +210 +00:16:04,760 --> 00:16:07,840 +اللي هم اللي .. اللي هي صنع ال collar العنق تبع + +211 +00:16:07,840 --> 00:16:11,940 +البكتيروفاج صنع المادة الوراثية كل الأهم تجمعهم مع + +212 +00:16:11,940 --> 00:16:15,920 +بعض عملية تجميع عند عملية التجميع لازم يكون في + +213 +00:16:15,920 --> 00:16:20,310 +مادة وراثية صح؟ لل .. للفيروسالمادة الوراثية ماذا + +214 +00:16:20,310 --> 00:16:25,230 +بياخدها هو؟ بياخدها من البكتيريا طب البكتيريا الآن + +215 +00:16:25,230 --> 00:16:29,610 +عشان توفر مادة وراثية مضطر الفيروس يحلل الكروموزوم + +216 +00:16:29,610 --> 00:16:32,690 +تبع البكتيريا فبتكسر الكروموزوم .. الكروموزوم + +217 +00:16:32,690 --> 00:16:37,770 +بتكسر فقد يكون هناك قطع من الكروموزوم مساوية في + +218 +00:16:37,770 --> 00:16:43,430 +الحجم لقطع من الجينوم تبع الفيروس فبدأ المياه يعبي + +219 +00:16:44,360 --> 00:16:48,240 +في ال .. في الرأس تبعته بدل ما يعبي قطعة من المادة + +220 +00:16:48,240 --> 00:16:51,060 +الورادية تبعته قد يعبي قطعة من مين؟ من ال + +221 +00:16:51,060 --> 00:16:55,800 +bacterial chromosome يبقى شكلا هو bacteriophage + +222 +00:16:55,800 --> 00:17:01,460 +لكن مضمونا هو .. هو مش bacteria هو .. هو مجرد حامل + +223 +00:17:01,460 --> 00:17:05,360 +معاه اللي هو المادة الورادية تعالي نطلع بشكل سريع + +224 +00:17:05,360 --> 00:17:11,960 +على الأعمالية دخل + +225 +00:17:12,750 --> 00:17:16,310 +واحد بالغلط وكنا من طبعا هو مش واحد في الغلط + +226 +00:17:16,310 --> 00:17:20,610 +الطبيعي بيصير مئات الفيروس هذا راح على ال + +227 +00:17:20,610 --> 00:17:23,870 +recipient cell بيفكر حاله مسكين بكتيروفاج عن جد + +228 +00:17:23,870 --> 00:17:29,310 +بيعمل injection الآن أخد قطعة من مين من ال donor و + +229 +00:17:29,310 --> 00:17:32,130 +أداها ال recipient صار لها integration في اللي هو + +230 +00:17:32,130 --> 00:17:35,990 +في ال recipient نكمل مع بعض هذا الآن بالبطيء + +231 +00:17:35,990 --> 00:17:41,050 +العملية together + +232 +00:17:41,050 --> 00:17:41,910 +assembly + +233 +00:17:44,530 --> 00:17:47,170 +البكتيروفاجي اللي قولنا بالغلط خدوا بقى كلمة + +234 +00:17:47,170 --> 00:17:52,490 +بالغلط بتعطينا .. بتعطينا نقطة مهمة جدا في المسمة + +235 +00:17:52,490 --> 00:17:59,250 +إنه أي قطعةقد تدخل بالخطأ وبالتالي ما بنعرفش + +236 +00:17:59,250 --> 00:18:02,890 +القطعة اللى انتقلت اى قطعة ولا انه جين فبسموه هذا + +237 +00:18:02,890 --> 00:18:05,750 +النوع من ال transduction اقرأوا على عنوانه هتلاقوا + +238 +00:18:05,750 --> 00:18:10,590 +مكتوب generalized transduction عام مش متخصص + +239 +00:18:10,590 --> 00:18:13,930 +مابنقدرش نتحكم في القطعة اللى بياخدها مين + +240 +00:18:13,930 --> 00:18:20,110 +البكتيريو فاجت على عكس نوع تانى طبعا قد نتحكم في + +241 +00:18:20,110 --> 00:18:24,350 +القطعة اللى بياخدها البكتيريو فاجتبس خدوا بالكم + +242 +00:18:24,350 --> 00:18:31,770 +المحصرة في البكتيريا الأولى لما دخل دخل بكل وضوح + +243 +00:18:31,770 --> 00:18:36,830 +جال انا مسئول نعم انا bacterophage و مسئول عن + +244 +00:18:36,830 --> 00:18:41,450 +الخلية و اللي بديها بعمله و في الآخر دمر الخلية و + +245 +00:18:41,450 --> 00:18:48,310 +طلع منها عمل lysis لذلك بيسموه lytic phageهذا + +246 +00:18:48,310 --> 00:18:51,490 +النوع من الفاجلين يسمونه Lightic Fascia إذا المن + +247 +00:18:51,490 --> 00:18:56,130 +المسئول عن ال generalized transduction هو ال + +248 +00:18:56,130 --> 00:19:00,650 +lightic fascia تعالوا نطلع على نوع تاني من ال + +249 +00:19:00,650 --> 00:19:06,090 +transduction و يسمى specialized transduction + +250 +00:19:06,090 --> 00:19:14,220 +أحكيلكوا القصة قبل أن نشوف ال animationالان فاكرين + +251 +00:19:14,220 --> 00:19:18,960 +لما البكتيروفاج نزل على البكتيريا زي المضالي وعمل + +252 +00:19:18,960 --> 00:19:22,560 +injection للمادة الوراثية دخل البكتيروفاج هذا + +253 +00:19:22,560 --> 00:19:28,720 +النوع وجالس السلام عليكم ممكن يعني تبسولي مكان في + +254 +00:19:28,720 --> 00:19:32,000 +ال chromosome انا مش لاجي مكان اقعد فيه اقعد في + +255 +00:19:32,000 --> 00:19:36,660 +هذا المكان فالبكتيريا بتقول اتفضل فيه لمكان خاص + +256 +00:19:36,660 --> 00:19:43,140 +بتجسه و بتقول لل genome تبقى واتفضل بتفضلالبكتيريا + +257 +00:19:43,140 --> 00:19:46,800 +بتتكثر و هو بتكثر مع البكتيريا كيف؟ بينقسم ال + +258 +00:19:46,800 --> 00:19:50,200 +chromosome تبع البكتيريا فبينقسم هو مع ال + +259 +00:19:50,200 --> 00:19:53,480 +chromosome يعني لو صار فيه من البكتيريا الأولى + +260 +00:19:53,480 --> 00:19:57,900 +مليون نسخة يبقى فيه عنده و هو كمان برضه مليون نسخة + +261 +00:19:57,900 --> 00:20:02,360 +صح ولا لأ؟ لأ المشكلة مش هنا المشكلة أنه صحيح + +262 +00:20:02,360 --> 00:20:05,220 +اتكثر مع البكتيريا و البكتيريا وفرتله حاضنة محترمة + +263 +00:20:05,220 --> 00:20:11,360 +و كويسة إذا شعر أن البكتيريا مهددة هو مش لاجي تاكل + +264 +00:20:11,360 --> 00:20:17,050 +البكتيريا مثلابطلع و بقولها انا .. مش انا .. انا + +265 +00:20:17,050 --> 00:20:21,730 +ال light اكترشبس المرة هاد ايش عمل هو؟ هو دخل + +266 +00:20:21,730 --> 00:20:26,430 +بهدوء و عمل integration و بعد ما تكاثر بفيه ملايين + +267 +00:20:26,430 --> 00:20:31,150 +الخلايا تمام؟ سحب نفسه، الان لما بيسحب نفسه بياخد + +268 +00:20:31,150 --> 00:20:36,170 +دايما معاه القطعة المجاورة لمين؟ من ال bacterial + +269 +00:20:36,170 --> 00:20:39,810 +chromosome فكل مرة لو دخلنا نفس ال bacterial فشل + +270 +00:20:39,810 --> 00:20:44,750 +هياخد نفس القطعة فبنسميه specialized transduction، + +271 +00:20:44,750 --> 00:20:45,950 +بنشوف مع بعض العمل + +272 +00:20:54,980 --> 00:20:58,940 +هي ال Fage DNA وهو Circular غالبا زى ما حكيتلكوا + +273 +00:20:58,940 --> 00:21:01,680 +الآن عملية ال integration بالدم بين وبين ال + +274 +00:21:01,680 --> 00:21:04,440 +chromosome تبع البكتيريا هى صار فيه ان integration + +275 +00:21:04,440 --> 00:21:15,600 +وفي هذه اللحظة مهم جدا نرجع ماعليش ايش + +276 +00:21:15,600 --> 00:21:20,220 +اسمه الآن؟لما ال bacteriophage يكون integrated في + +277 +00:21:20,220 --> 00:21:23,980 +الخلية بنسميه Prophage خدوا بالكوا الخلية المحتوية + +278 +00:21:23,980 --> 00:21:30,460 +على ال Prophage بنسميها Lysogenized Cell يعني خلية + +279 +00:21:30,460 --> 00:21:36,220 +فيها .. هتمر المصطلح هتمر معاكوا بعد قريب الخلية + +280 +00:21:36,220 --> 00:21:40,660 +اسمها Lysogenized Cell وهدي برضه الفائدة الثالثة + +281 +00:21:40,660 --> 00:21:45,920 +اللي حكيناها اليوم عن ال bacteriophageالتانية + +282 +00:21:45,920 --> 00:21:52,540 +treatment التالتة pathogenesis تخيلوا + +283 +00:21:52,540 --> 00:21:56,980 +أن البكتيريو ال بروفاج هذا بيحمل في طياته جين + +284 +00:21:56,980 --> 00:22:02,170 +لتكسينلما البكتيريا بيصير replication لل DNA، + +285 +00:22:02,170 --> 00:22:04,730 +بيصير بعد ذلك transcription، إيش بعد ال + +286 +00:22:04,730 --> 00:22:09,030 +transcription؟ Translation، فهتصير تنتج ال toxin + +287 +00:22:09,030 --> 00:22:14,130 +اللي هو أصلا من وين جاي من البكتيريا و فعشها، + +288 +00:22:14,130 --> 00:22:17,890 +بكتيريا ماعنداش مقدرة تنتج toxin، أصلا لديها + +289 +00:22:17,890 --> 00:22:23,100 +المقدرة تنتج toxin، أعطيكم مثال عليها؟الـ + +290 +00:22:23,100 --> 00:22:27,160 +Cornebacterium Diphtheria اللي بتسبب مرض الـ + +291 +00:22:27,160 --> 00:22:31,880 +Diphtheria هذه البكتيريا أساسا غير ممرضة أصبحت + +292 +00:22:31,880 --> 00:22:35,780 +ممرضة لما صارت lysogenized يعني لما صار فيها + +293 +00:22:35,780 --> 00:22:40,920 +بروفاج عطاها المخدر اللي انتج منه اللي هو الـToxin + +294 +00:22:40,920 --> 00:22:46,400 +وعلى فكرة مجموعة ضخمة من البكتيريا الممرضة هي + +295 +00:22:46,400 --> 00:22:51,540 +ممرضة لأن فيها بروفاجهي تلاتة افكارات مهمة جدا + +296 +00:22:51,540 --> 00:22:54,560 +اليوم أخدناهم عن ال bacteriophage، diagnosis و + +297 +00:22:54,560 --> 00:22:59,660 +treatment و في ال pathogenesis، نكمل بروفاج اللي + +298 +00:22:59,660 --> 00:23:03,600 +بقى عند بكتيريا بتنقسم جهدها ملايين المرات، عند + +299 +00:23:03,600 --> 00:23:10,000 +ظرف معين ال bacteriophage هيبدأ إيه؟ يقرر بده + +300 +00:23:10,000 --> 00:23:13,960 +ينفصل، مين أخد معاه؟ قطعة من اللي هو ال + +301 +00:23:13,960 --> 00:23:14,800 +bactericromosome + +302 +00:23:17,370 --> 00:23:22,350 +بعمل الآن نفس الخطوات اللى عملها مع ال light cycle + +303 +00:23:22,350 --> 00:23:27,010 +هاد + +304 +00:23:27,010 --> 00:23:35,290 +اللى أنا عملت ال assembly بعمل license للخلية طبعا + +305 +00:23:35,290 --> 00:23:38,250 +هاد ال animation أنا هزلكيه ان شاء الله على الموقع + +306 +00:23:38,250 --> 00:23:44,040 +و فيه صوت يعني ممكن ان الشرح يكون commandryالأن هو + +307 +00:23:44,040 --> 00:23:46,960 +من donor cell را على recipient cell اللي هي + +308 +00:23:46,960 --> 00:23:50,860 +المستقبل بيعمل injection للمادة الوراثية دباته و + +309 +00:23:50,860 --> 00:23:53,620 +بيصير لها integration الان صار في البكتيريا + +310 +00:23:53,620 --> 00:23:59,480 +الجديدة صفات جديدة من مصدرين مختلفين مصدر الأولاني + +311 +00:23:59,480 --> 00:24:04,980 +البكتيريا النفاش نفسه قد يكون مصدر و الجزء اللي + +312 +00:24:04,980 --> 00:24:08,240 +أخده من البكتيريا الأخرى كمان مصدر آخر فصار عنده + +313 +00:24:08,240 --> 00:24:12,960 +الآن تلت مصادر للمعلومات ال originalو ال + +314 +00:24:12,960 --> 00:24:15,080 +bacteriophage نفسه البكتيريا نفسنا او البكتيريا + +315 +00:24:15,080 --> 00:24:15,960 +نفسنا او البكتيريا نفسنا او البكتيريا نفسنا او + +316 +00:24:15,960 --> 00:24:17,880 +البكتيريا نفسنا او البكتيريا نفسنا او البكتيريا + +317 +00:24:17,880 --> 00:24:18,160 +نفسنا او البكتيريا نفسنا او البكتيريا نفسنا او + +318 +00:24:18,160 --> 00:24:19,000 +البكتيريا نفسنا او البكتيريا نفسنا او البكتيريا + +319 +00:24:19,000 --> 00:24:21,740 +نفسنا او البكتيريا نفسنا او البكتيريا نفسنا او + +320 +00:24:21,740 --> 00:24:25,540 +البكتيريا نفسنا او البكتيريا نفسنا او البكتيريا + +321 +00:24:25,540 --> 00:24:28,900 +نفسنا او البكتيريا نفسنا او البكتيريا نفسنا او + +322 +00:24:28,900 --> 00:24:39,040 +البكتيريا نفسنا او البكتيريا نفسنا او البكتيريا + +323 +00:24:42,380 --> 00:24:49,420 +أحد تطبيقات الان + +324 +00:24:49,420 --> 00:24:53,400 +gene R اختصار ليش R؟ R غالبا في ال .. في ال .. في + +325 +00:24:53,400 --> 00:24:56,960 +ال microbial resistance طبعا هنجيله اتصالات مهان + +326 +00:24:56,960 --> 00:25:02,900 +أخرى روف على سبيل المثال روف يعني خشن لكن لو + +327 +00:25:02,900 --> 00:25:08,440 +البكتيرياالدولار كان فيها resistant gene للبنسالين + +328 +00:25:08,440 --> 00:25:12,460 +نفترض للبنسالين ونقلنا بالباتريوفاج عبر + +329 +00:25:12,460 --> 00:25:16,360 +transduction تمام هذا ال gene إلى خلية أخرى هذه + +330 +00:25:16,360 --> 00:25:19,400 +الخلية كانت sensitive للبنسالين تلقيا هى بتصير + +331 +00:25:19,400 --> 00:25:23,880 +resistantالان نقلت من خلية الى خلية احنا حاجب + +332 +00:25:23,880 --> 00:25:27,820 +نسميها horizontal gene transfer ايش يعني + +333 +00:25:27,820 --> 00:25:32,680 +horizontal؟ قفقي من خلية الى خلية ال vertical هو + +334 +00:25:32,680 --> 00:25:36,640 +الانقسام انه خلية تنقسم تبتعد تتود و تترسل نفس + +335 +00:25:36,640 --> 00:25:41,680 +الشيء بس ال transduction و ال transformation و ال + +336 +00:25:41,680 --> 00:25:44,840 +conjugation يسمى ايش الان؟ horizontal gene + +337 +00:25:44,840 --> 00:25:49,140 +transfer كمل + +338 +00:25:52,450 --> 00:25:59,250 +كانت البكتيريا resistant، أخدت البكتيروفاج، + +339 +00:25:59,250 --> 00:26:04,370 +هيروح عندنا على .. على recipient cell كتانية و + +340 +00:26:04,370 --> 00:26:07,370 +يخليها resistant لل antibiotic اللي كان resistant + +341 +00:26:07,370 --> 00:26:08,850 +له الخلية الأولى + +342 +00:26:13,170 --> 00:26:16,870 +طيب عشان احنا يعني ان شرحنا ال transformation و + +343 +00:26:16,870 --> 00:26:23,770 +ماوضعناش عليها اي توضيحات هاي قدامنا توضيح هذه ال + +344 +00:26:23,770 --> 00:26:28,730 +competent cell ايش يعني competent cell competent + +345 +00:26:28,730 --> 00:26:33,450 +cell عكسها باللغة لل non-competent cell ال non + +346 +00:26:33,450 --> 00:26:37,650 +-competent cell هي الخلية الغير جاهزة لا استقبالت + +347 +00:26:37,650 --> 00:26:38,050 +يعني + +348 +00:26:42,380 --> 00:26:44,720 +خد بالك في المعمل ممكن نخليها competent احنا + +349 +00:26:44,720 --> 00:26:51,820 +بالكهربا يعني نكهربها او نحط عليها مواد ملحية + +350 +00:26:51,820 --> 00:26:54,780 +ونخليها جاهزة يعني ممكن احنا نعالجها معالجة + +351 +00:26:54,780 --> 00:26:59,060 +ونخليها جاهزة ال competence اللي هي نخليها جاهزة + +352 +00:26:59,060 --> 00:27:03,800 +لاستقبال المادة دي ورا تعالوا نطلع مع بعض على + +353 +00:27:03,800 --> 00:27:04,600 +الفيديو + +354 +00:27:08,790 --> 00:27:11,650 +اللي بيورينا ال competent cell و حواليها fragment + +355 +00:27:11,650 --> 00:27:15,190 +من ال DNA، يقولنا من مصادر متنوعة و مختلفة، مش + +356 +00:27:15,190 --> 00:27:20,050 +ضروري بكتيريا، مش ضروري بكتيريا، من أي حاجة، بيصير + +357 +00:27:20,050 --> 00:27:24,650 +intake للمادة الوراثية، وبعدين بيصيرها + +358 +00:27:24,650 --> 00:27:26,990 +recombination، اعتقد ان كلمة recombination صارت + +359 +00:27:26,990 --> 00:27:28,190 +واضحة، اتحاد + +360 +00:27:33,850 --> 00:27:36,610 +صفة جديدة ولا مش صفة جديدة؟ تغيير وراتي ولا مش + +361 +00:27:36,610 --> 00:27:40,310 +تغيير وراتي؟ تغيير وراتي طبعا احنا في المختبرات + +362 +00:27:40,310 --> 00:27:46,530 +ممكن نكون بالعملية هذه طمعا في إدخال جينات معينة + +363 +00:27:46,530 --> 00:27:51,070 +للبكتيريا بغلط تحسينها أو بغلط إنتاج .. منتج معين + +364 +00:27:51,070 --> 00:27:54,810 +يعني ممكن نكون بهذه العملية تعالوا نطلع على اللوحة + +365 +00:27:54,810 --> 00:27:57,910 +الرائعة هذه اللي قدامنا وخلّيني أشرحها قبل ما + +366 +00:27:57,910 --> 00:28:03,290 +أتكلم أو قبل ما أكملهاأنا قولت في عندي R بمعناين، + +367 +00:28:03,290 --> 00:28:08,770 +R resistant و هنا R rough، rough عكس smooth، + +368 +00:28:08,770 --> 00:28:12,090 +smooth عنها نعم، في بكتيرية اسمها streptococcus + +369 +00:28:12,090 --> 00:28:15,890 +pneumonia حاكينا مرة اظنلي عنهاال street gogas + +370 +00:28:15,890 --> 00:28:19,070 +pneumonia في منها two variants صينفين واحدة ب + +371 +00:28:19,070 --> 00:28:23,210 +capsule واحدة بدون capsule مستعمراتهم على الطبق + +372 +00:28:23,210 --> 00:28:27,270 +بيبدو يعني soft smooth اللي لها capsule و اللي + +373 +00:28:27,270 --> 00:28:33,590 +مالاش capsule لتبدو soft بسموها rough لما حققنا ال + +374 +00:28:33,590 --> 00:28:40,230 +rough في ال mice الفار يعني الفار ماصارله اشي لما + +375 +00:28:40,230 --> 00:28:48,110 +حققنا ال s في ال mice مات الفارالـR، الـR non + +376 +00:28:48,110 --> 00:28:51,930 +-pathogenic، بقناة أن البكتيريا حيّة، البكتيريا + +377 +00:28:51,930 --> 00:28:55,970 +نفسها حيّة، + +378 +00:28:55,970 --> 00:28:58,150 +أنا أعرف أن البعض ممكن يتقلل أحيانًا على هو ال + +379 +00:28:58,150 --> 00:29:07,230 +injection، لكن الفار مابعرف، الآن حقنّها بالـSاللي + +380 +00:29:07,230 --> 00:29:12,110 +هو ال smooth اللي له كابسول الفقر نات و استنتجنا + +381 +00:29:12,110 --> 00:29:15,490 +إنه ولود الكابسول virulence factor حكينا عنه لما + +382 +00:29:15,490 --> 00:29:21,190 +تكلمنا عن ال structure لل bacterial cell نات الفقر + +383 +00:29:21,190 --> 00:29:28,890 +تجربة تانية الحين جبنا جبنا اللي هو ال ال ال ال + +384 +00:29:28,890 --> 00:29:33,250 +smooth و قتلناها بالحرارة و حقلناها في الميسر + +385 +00:29:38,840 --> 00:29:44,240 +انتوا عملت غبارة، يعني موت او مجموعة، بقى هاجلبة، + +386 +00:29:44,240 --> 00:29:49,280 +اللي قال تجلبة الرابعة، اه تبقى كويس، هذا الخطوة + +387 +00:29:49,280 --> 00:29:55,260 +الحاسمة، ال rough حية و ال smooth ميتة، heat + +388 +00:29:55,260 --> 00:30:03,060 +killed، برضه هيعيش لأنه اه، لأ، هيموت، احنا مصممين + +389 +00:30:03,060 --> 00:30:06,340 +التجلبة عشان يموتعشان نثبتلكوا أنه .. عشان + +390 +00:30:06,340 --> 00:30:10,080 +نثبتلكوا أنه ال .. ال rough .. ال rough اللي هي + +391 +00:30:10,080 --> 00:30:16,120 +الحية أخدت صفات من ال smooth اللي هي الميتة و عبر + +392 +00:30:16,120 --> 00:30:19,260 +إيش أخدتها؟ عبر ال transformation لإن لا مافيه + +393 +00:30:19,260 --> 00:30:22,600 +بكتيروفاج ولا فيه ply اللي هي ال conjugation اللي + +394 +00:30:22,600 --> 00:30:24,860 +بنعرفها ال conjugation وبالتالي شوفوا الآن إيش + +395 +00:30:24,860 --> 00:30:29,520 +اللي بصير الفار لما حقنناها بالخلطة هذه اللي هي ال + +396 +00:30:29,520 --> 00:30:36,010 +R الحية و ال smooth الميتة ماد الفارعلى عكس توقعك + +397 +00:30:36,010 --> 00:30:40,090 +وانت ولمّا عزلنا منه البكتيريا ما عزلنا ال R، إيش + +398 +00:30:40,090 --> 00:30:44,450 +عزلنا؟ ال smooth أظن صارت الصورة واضحة الآن إنه + +399 +00:30:44,450 --> 00:30:49,310 +something was transferred من ال smooth الميتة إلى + +400 +00:30:49,310 --> 00:30:53,930 +الرف الحية جعلتها smooth إيش هذا الانتقال؟ ال gene + +401 +00:30:53,930 --> 00:30:58,970 +تبع اللي هو إنتاج ال capsule هذا تجربة على فكرة + +402 +00:30:58,970 --> 00:31:02,310 +وإن كانت بسيطة إلا إنه في منها معلومات كبيرة كتير + +403 +00:31:02,310 --> 00:31:05,880 +لازمنا نستقيهاإن المادة الواقعية هي ال DNA حقا لدى + +404 +00:31:05,880 --> 00:31:09,720 +DNA إنه في تجربة تانية أخد بس ال DNA و حطوه مع ال + +405 +00:31:09,720 --> 00:31:15,400 +air level إن ال capsule is a virulence factor صح و + +406 +00:31:15,400 --> 00:31:20,500 +ده ده؟ و ده كتير نقطة مهمة أفرجت بين ال stripped + +407 +00:31:20,500 --> 00:31:24,140 +coccus pneumonia ال S و ال R القتل و عدم القتل + +408 +00:31:24,140 --> 00:31:26,860 +الموت و عدم الموت الإمراض و عدم الإمراض وجوده و + +409 +00:31:26,860 --> 00:31:30,200 +عدم وجوده اللى هو ال cash أرجو أنكوا تكونوا + +410 +00:31:38,050 --> 00:31:43,290 +عشان احنا مانظمكوش بنوريكم ال conjugation كمان مرة + +411 +00:31:43,290 --> 00:31:49,130 +و نأكد على ان احنا شفنا العملية كاملةهذا ال F + +412 +00:31:49,130 --> 00:31:53,050 +positive تمام؟ و ال F negative صار فيه بينهم + +413 +00:31:53,050 --> 00:31:58,770 +ارتباط بدأ الارتباط يكون اكتر intimate بعدين انتقل + +414 +00:31:58,770 --> 00:32:02,850 +ال F positive بلازميت الى ال F negative و صار فيه + +415 +00:32:02,850 --> 00:32:06,030 +يعني double strand للتانين ال F negative ايش صارت + +416 +00:32:06,030 --> 00:32:11,510 +اللي اعني؟ F positive و بدأت هي تعمل conjugation ل + +417 +00:32:11,510 --> 00:32:16,050 +.. ل .. ل .. ل F negative self وهذا اللي هو ال + +418 +00:32:16,050 --> 00:32:24,260 +conjugation المعروفالابيزوم، فاكرين الابيزوم؟ هيها + +419 +00:32:24,910 --> 00:32:28,210 +اللي هي ال .. ال .. ال .. ال .. ال plasmid متحدة + +420 +00:32:28,210 --> 00:32:31,870 +مع مين؟ مع ال chromosome و لاحظوا في ال .. في + +421 +00:32:31,870 --> 00:32:34,650 +الخطوة .. في المربع التالت أن ال chromosome .. ال + +422 +00:32:34,650 --> 00:32:38,110 +chromosome بدأ ينتقل مع مين؟ مع اللي هو .. مع ال + +423 +00:32:38,110 --> 00:32:42,850 +ebisome نفسه و هاد يقولنا ساموها HFR أو high + +424 +00:32:42,850 --> 00:32:46,110 +frequency recombination لإنه فينا كمية كبيرة من + +425 +00:32:46,110 --> 00:32:49,550 +مين؟ من المادة الوراثية اللي بينتقل مش .. مش زي ال + +426 +00:32:49,550 --> 00:32:52,830 +plasmid ال plasmid قطعة صغيرة بالمقارنة مع ال + +427 +00:32:52,830 --> 00:32:59,560 +chromosomeيبقى تلت طرق .. تلت طرق من ال gene + +428 +00:32:59,560 --> 00:33:04,060 +transfer بدكوا تكونوا عارفينهم بشكل جيد لإنه مهمين + +429 +00:33:04,060 --> 00:33:08,340 +جدا في حياتنا بنعرف إن البتكيلة اتغيرت لمجموعة من + +430 +00:33:08,340 --> 00:33:12,100 +الأسباب واحد من هذه الأسباب ال mutation السبب + +431 +00:33:12,100 --> 00:33:14,660 +التاني الرئيسي ال gene transfer اللي هم التلات + +432 +00:33:14,660 --> 00:33:18,840 +اللي ذكرناها ال .. ال .. ال .. الجزء الرابع اللي + +433 +00:33:18,840 --> 00:33:20,680 +هو ال .. ال protoplast fusion + +434 +00:33:24,970 --> 00:33:28,170 +اعتقد ان المصطلح مش غريب وانا شبحته لان اتكلمت عن + +435 +00:33:28,170 --> 00:33:32,450 +ال cell wall لما قلت ان البكتيريا اللى من غير cell + +436 +00:33:32,450 --> 00:33:35,750 +wall بنسميها protoplast خاصة اللى بنصنعها احنا + +437 +00:33:35,750 --> 00:33:40,090 +وكان فينا صديق يدرس معانا في مدستير فكان احد + +438 +00:33:40,090 --> 00:33:46,140 +التجارب طلعته في البحث ان يدمج two cellsال two + +439 +00:33:46,140 --> 00:33:50,760 +cells اللي بذكره ان واحدة stable واحدة منهم stable + +440 +00:33:50,760 --> 00:33:54,660 +و بتنتج كمية كبيرة من ال antibiotic و التانية + +441 +00:33:54,660 --> 00:33:57,720 +بتنتج كمية كبيرة من ال antibiotic بس مش ايه مش + +442 +00:33:57,720 --> 00:34:01,490 +stableفالميزة اللي هنا عيب في هذه و العيب اللي هنا + +443 +00:34:01,490 --> 00:34:05,230 +أو الميزة اللي هنا عيب في التانية، فقال أو كان + +444 +00:34:05,230 --> 00:34:09,170 +بيفكر أنه لو دمجهم اتنتين مع بعض، تمام؟ قد يحصل + +445 +00:34:09,170 --> 00:34:13,090 +على خلية تمتلك الصفتين الإيجابيتين، و بالفعل هذا + +446 +00:34:13,090 --> 00:34:15,830 +ما حدث في هذا كان يمكن .. يمكن أن كان الأفضل جزء + +447 +00:34:15,830 --> 00:34:19,270 +في رسالة feministية تبعه، و العملية بيسميها plot + +448 +00:34:19,270 --> 00:34:22,910 +of last في ذلك اللحظةالميزة والعيب كانت نتيجة عن + +449 +00:34:22,910 --> 00:34:26,590 +مين؟ عن التركيب الولادة لما احنا اتسبنا الصفتين مع + +450 +00:34:26,590 --> 00:34:30,390 +بعض ايه صار عندنا؟ صار عندنا تغيير في الجينات + +451 +00:34:30,390 --> 00:34:34,450 +وبالتالي العنوان mechanism of bacterial variation + +452 +00:34:34,450 --> 00:34:39,770 +من اين يأتي اختلاف الجينات في اللي هو البكتيريات + +453 +00:34:39,770 --> 00:34:43,030 +mutation gene transfer protoplast fusion + +454 +00:34:48,820 --> 00:34:53,620 +الـ protoplast fusion عبارة عن two cells، بنشيل ال + +455 +00:34:53,620 --> 00:34:55,840 +cell wall تبعهم، اللي كان اللي هم cell wall، تمام؟ + +456 +00:34:55,840 --> 00:34:59,900 +اللي كان اللي هم cell wall، و بنحط معاهم مادة + +457 +00:34:59,900 --> 00:35:04,100 +بسموها fusing agent، زي polyethylene glycol، + +458 +00:35:04,100 --> 00:35:09,140 +polyethylene glycol، أو بختصروا PEG، polyethylene + +459 +00:35:09,140 --> 00:35:13,500 +glycolهذه المادة بتحب تدمج الخلايا مع بعضها + +460 +00:35:13,500 --> 00:35:17,380 +فالخلايا بندمج و بيصيروا بدل ما هم خاليتين بيصيروا + +461 +00:35:17,380 --> 00:35:21,720 +خالية واحدة و ال recombination فاكرين كلمة + +462 +00:35:21,720 --> 00:35:24,860 +recombination؟ اللي هو ارتباط مادة و راطية من مصدر + +463 +00:35:24,860 --> 00:35:28,360 +لمصدر آخر اللي بيشتغل بحيث انه مايكون إلا في + +464 +00:35:28,360 --> 00:35:32,860 +كروموزوم واحد في الخلية، في خلاية البكتيريا هذا + +465 +00:35:32,860 --> 00:35:35,300 +الكروموزوم غالبا بيكون من مين؟ خليط من ال two + +466 +00:35:35,300 --> 00:35:41,230 +cellsفالان صرا عندي خاليتين او كان في عندي خاليتين + +467 +00:35:41,230 --> 00:35:47,550 +صاروا خالية واحدة بكرومترا طب احنا شرحنا ال gene + +468 +00:35:47,550 --> 00:35:52,850 +transfer و ما شرحنا ال mutation و هنجف عند ال + +469 +00:35:52,850 --> 00:35:59,550 +mutation طويلا لانه مهم جدا نعرف نوعين من ال + +470 +00:35:59,550 --> 00:36:03,310 +mutation اسمهم base, substitution و حاجة اسمها + +471 +00:36:03,310 --> 00:36:11,460 +friendshipو ال frame shift معناها إزاحة و الإزاحة + +472 +00:36:11,460 --> 00:36:18,420 +قد تمتج عن إضافة أو حدث إضافة أو حدث و عندي الصحية + +473 +00:36:18,420 --> 00:36:22,940 +أنا animation كويسة بتوريكم إياها بتوضح + +474 +00:36:28,260 --> 00:36:32,600 +بس هي حابة اتركزوا مع ال presentation اكتر من + +475 +00:36:32,600 --> 00:36:37,880 +اتركزوا مع الكتابة لإن الفكرة فيها كتير واضحة، هذه + +476 +00:36:37,880 --> 00:36:41,940 +المادة الوراثية مكوّنة من قواعد نيتروجينية، كل + +477 +00:36:41,940 --> 00:36:46,860 +ثلاث قواعد تشكل شفرة، ال codon بالظبط، و ال codon + +478 +00:36:46,860 --> 00:36:53,410 +يعني شيءلكن الشيء قد يُرمز له بأكتر من كودون، صح؟ + +479 +00:36:53,410 --> 00:37:01,990 +يعني مثلا الـ stop codons UAA, UAG و UGA، كويسة + +480 +00:37:01,990 --> 00:37:04,550 +النقطة دي، لإنه بتفيدنا الآن في ال mutation، بعض + +481 +00:37:04,550 --> 00:37:11,210 +الأحيان ال UAG لو اتغيرت ل UAA، صحيح صارت أفرق، + +482 +00:37:11,210 --> 00:37:14,510 +لكن هي نفس ال stop codon، و تالي ما صارش أنها أي + +483 +00:37:14,510 --> 00:37:16,430 +تغيير، فبسمو ال mutation silent + +484 +00:37:25,860 --> 00:37:33,660 +هذا طبعا اللي هو الديانيك اللي مرايز بيشتغل زي + +485 +00:37:33,660 --> 00:37:37,740 +ما احنا احنا بعض الأحيان نطبع على الحاسوب و بعدين + +486 +00:37:37,740 --> 00:37:42,590 +نرجع ندققال DNA في الملايز عنده editing يعني عملية + +487 +00:37:42,590 --> 00:37:45,610 +تحرير للأخطاء إذا كان فيه عنده أخطاء لكن لا يوجد + +488 +00:37:45,610 --> 00:37:50,170 +hundred percent proof فقلنالكوا بيصير أخطاء و + +489 +00:37:50,170 --> 00:37:55,310 +سمينا الأخطاء دي اللي هي ال spontaneous mutation و + +490 +00:37:55,310 --> 00:37:58,050 +أنا بقولي هذه الأخطاء يمكن بيبقى .. بيبقى نشتدرنا + +491 +00:37:58,050 --> 00:38:02,280 +احنا كباشر أخطاء قد تكون مبرمجة هتحصللأن احنا + +492 +00:38:02,280 --> 00:38:05,640 +بنلاحظ أن ال mutation في البكتيريا غالبا بتفيد + +493 +00:38:05,640 --> 00:38:08,860 +البكتيريا، البكتيريا بتستفيد من هذه ال mutations، + +494 +00:38:08,860 --> 00:38:13,620 +قد تحسن من قدراتها على ال adaptation مع ال + +495 +00:38:13,620 --> 00:38:17,640 +environment، تخيلوا الآن الوضع التالي، احنا في + +496 +00:38:17,640 --> 00:38:24,080 +كوكب إنه 150 سنة بنصنع مركبات synthetic، مش + +497 +00:38:24,080 --> 00:38:24,560 +natural + +498 +00:38:27,100 --> 00:38:30,520 +إيش يعني مش natural؟ يعني .. يعني بمعنى آخر non + +499 +00:38:30,520 --> 00:38:36,940 +-biodegradable يعني غير قابل للتكسير كمان 150 سنة + +500 +00:38:36,940 --> 00:38:40,500 +من هذه المركبات قد يمتلق كوكبنا .. قد يمتلق كوكبنا + +501 +00:38:40,500 --> 00:38:45,820 +بالمخلفات و النفايات تبع هذه المنتجات وجود + +502 +00:38:45,820 --> 00:38:49,580 +بكتيريا قادرة على التاكله .. الآن احنا بنعرف إن + +503 +00:38:49,580 --> 00:38:51,900 +فيه بكتيريا بتاكل ال plastic و ال plastic + +504 +00:38:55,260 --> 00:39:00,160 +Synthetic اكتر من المبيضات الحشرية اللى هى + +505 +00:39:00,160 --> 00:39:04,320 +Synthetic أساسا و non-biodegradable بنروح على + +506 +00:39:04,320 --> 00:39:08,200 +أماكن رشها اللى وان بيرشوها باستمرار و بناخد + +507 +00:39:08,200 --> 00:39:12,840 +بكتيريا من المنطقة هناك تمام و بنحاول نعمل وسط + +508 +00:39:12,840 --> 00:39:16,420 +زراعى هذا الوسط الزراعى مافيش فيه مصدر كربوني + +509 +00:39:16,420 --> 00:39:19,100 +فاكدوا ان هذا الكربوني لافيش لاكود ولا فيه نشاط + +510 +00:39:19,100 --> 00:39:24,670 +بحطلها المبيض مصدر كربونيوبنزرع البكتيريا اللى + +511 +00:39:24,670 --> 00:39:29,030 +موجودة من الطربة على الطبق هذا إذا البكتيريا بتكسر + +512 +00:39:29,030 --> 00:39:32,550 +المصدر الكربوني حتى إنه بشكل مصدر عموما فبنصير + +513 +00:39:32,550 --> 00:39:35,370 +نازل البكتيريا وعزلنا بكتيريا بالفعل اللى مقدرة + +514 +00:39:35,370 --> 00:39:39,990 +على تكسير المبدال هذا يمكن يكون نازل عن إيه؟ عن + +515 +00:39:39,990 --> 00:39:44,770 +spontaneous فال spontaneous من ورقة نظرنا احنا انه + +516 +00:39:44,770 --> 00:39:48,410 +..لأ مش سيء انه ..انه بتون سبب لكن قد يكون + +517 +00:39:48,410 --> 00:39:51,790 +البكتيريا مبرمجة ان يصير فيها spontaneous mutation + +518 +00:40:04,800 --> 00:40:07,800 +بنكمل نوعين الميمتاجين الـ Bay Substitution اللي + +519 +00:40:07,800 --> 00:40:11,740 +هي إحلال لقاعدة و Frame Shift مفيش أنها غير + +520 +00:40:11,740 --> 00:40:15,060 +النوعين دول اللي موجودين قدامك الآن بيكونوا طالعوا + +521 +00:40:15,060 --> 00:40:17,100 +على اللوحة هذه نعمل stop + +522 +00:40:26,980 --> 00:40:33,900 +قدامنا DNA و المقابل تبعاش هكون mRNA و بعد ال mRNA + +523 +00:40:33,900 --> 00:40:36,880 +هكون اللي هو ال protein نطلع على ال sequence + +524 +00:40:36,880 --> 00:40:40,700 +فينالألانين تيروسين ألانين و أرجانين مظبوط هي + +525 +00:40:40,700 --> 00:40:44,160 +الأربعة الموجودين بين العالمين على تتابع الحلقات + +526 +00:40:44,160 --> 00:40:48,160 +اللي موجودة في ال DNA و من ثم في اللي هو ال RNA + +527 +00:40:48,160 --> 00:40:53,500 +الآن بدنا نشوف إيش تأثير ال base substitution أو + +528 +00:40:53,500 --> 00:40:56,040 +احتمالات تأثير ال base substitution + +529 +00:40:59,690 --> 00:41:04,630 +نقف أول احتمال اللي هو silent mutation تفكرين ما + +530 +00:41:04,630 --> 00:41:08,410 +هو silent mutation؟ اللي يقول عنها انه تغيير ينتج + +531 +00:41:08,410 --> 00:41:13,550 +عنه عدم تغيير في الرمز .. مش في الرمز في ال .. في + +532 +00:41:13,550 --> 00:41:16,650 +ال .. في ال .. في المرموز لهو في المرموز لهو اللي + +533 +00:41:16,650 --> 00:41:20,010 +هو ال amino acid أو إذا كان stop codon إلى آخر + +534 +00:41:20,010 --> 00:41:27,710 +وهذا لن يؤثر على تتابعالأمينو assets وبالتالي ال + +535 +00:41:27,710 --> 00:41:32,550 +mutation موجودة لكن لم يظهر لها أي تأثير و بيسميها + +536 +00:41:32,550 --> 00:41:39,850 +silent mutation النوع التاني لأ ال codon احنا قلنا + +537 +00:41:39,850 --> 00:41:43,910 +انه base واحدة بتتغير صح شيلنا base و حطينا base + +538 +00:41:43,910 --> 00:41:48,530 +الان لما تغيرت ال base تتغير ال amino acidيبقى صار + +539 +00:41:48,530 --> 00:41:53,710 +عندي هانال أنا أعيش هنا لحظة بدل الالانين صار عندي + +540 +00:41:53,710 --> 00:41:58,970 +ال glycine يبقى صار فيه اختلاف اخدوا بالكم مش يعني + +541 +00:41:58,970 --> 00:42:01,490 +ان صار فيه اختلاف اقل من قصدي يعني الاختلاف بسيط + +542 +00:42:01,490 --> 00:42:06,750 +قد يكون الاختلاف drastic يعني بيبقى له تأثير قد + +543 +00:42:06,750 --> 00:42:11,050 +يعني يجعل من ال protein non-functional وقد يجعله + +544 +00:42:11,050 --> 00:42:16,470 +اقل functional وقد لايؤثر على ال functional البتة + +545 +00:42:16,470 --> 00:42:20,440 +يعنيما بنعرفش بالضبط إيش التأثير، لكن في الآخر فيه + +546 +00:42:20,440 --> 00:42:26,280 +تأثير، فيه تأثير Non-sense mutation بدل ما يتغير + +547 +00:42:26,280 --> 00:42:31,660 +الكودون لرمز الأمينواسد، بتغير ل stop-kodon، فبصير + +548 +00:42:31,660 --> 00:42:34,620 +لما يقرأوا ال RNA polymerase عنده يوجّه، وبالتالي + +549 +00:42:34,620 --> 00:42:37,940 +إيش ال protein اللي بيطلع هنا؟ protein ناجس، هذا + +550 +00:42:37,940 --> 00:42:39,760 +ال protein ناجس إيش بنسميه؟ إنه واحدة من ال + +551 +00:42:39,760 --> 00:42:43,740 +mutation non-sense، كلام فاضي، كلام فارغ، تمام؟ + +552 +00:42:43,740 --> 00:42:48,690 +يعني protein مش تمام، مش تمامهذا الان احنا اللي + +553 +00:42:48,690 --> 00:42:52,650 +اتعلمنا اللي هو ال base substitution base + +554 +00:42:52,650 --> 00:42:59,770 +substitution يا اما silent يا اما missings او non + +555 +00:42:59,770 --> 00:43:03,910 +missings نيجي الان لمين؟ لل frame shift قبل ان + +556 +00:43:03,910 --> 00:43:07,490 +ننتقل لل frame shift احنا عندنا سطر من الطالبات + +557 +00:43:07,490 --> 00:43:12,050 +اللي امامنا بهذا الشكل واحد اتنين تلاتة اربعة خمسة + +558 +00:43:12,050 --> 00:43:17,950 +ستة جبنا كرسي و حطينا بعد لقم اتنينإيش كان ترتيبها + +559 +00:43:17,950 --> 00:43:19,850 +التانية؟ كانت ترتيبها التانية الآن إيش صار + +560 +00:43:19,850 --> 00:43:23,010 +ترتيبها؟ تلاتة و اللي بعدها كانت تلاتة صارت أربعة + +561 +00:43:23,010 --> 00:43:28,050 +يبقى كل ال sequence اللي بعد الإضافة أو بعد الحذف + +562 +00:43:28,050 --> 00:43:31,510 +هيتغير هيحسب منها frame shift نوع من إيه؟ من + +563 +00:43:31,510 --> 00:43:35,710 +الإزاحة اللي قلنا كانت اتنين صارت تلاتة أو اللي + +564 +00:43:35,710 --> 00:43:38,790 +كانت اتنين صارت واحد يعني على حسب اللي هو هل هو + +565 +00:43:38,790 --> 00:43:42,850 +addition ولا deletion هل هو insertion ولا deletion + +566 +00:43:42,850 --> 00:43:48,630 +وهذا هو ما يسمى بال frame shiftطبعا تخيلوا أنه + +567 +00:43:48,630 --> 00:43:52,390 +تغيير واحد أدى إلى تغيير الكل اللي بعده، صح ولا + +568 +00:43:52,390 --> 00:43:55,510 +لأ؟ وبتالي غالبا تأثير ال frame shift برضه تأثير + +569 +00:43:55,510 --> 00:43:55,990 +قوي + +570 +00:44:06,610 --> 00:44:10,130 +تعالوا تطلعوا على التأثير القوي، بس أول amino acid + +571 +00:44:10,130 --> 00:44:13,410 +داي زي ما هو، أول amino acid داي زي ما هو ال bag + +572 +00:44:13,410 --> 00:44:16,550 +إيش صار فيهم، اتغيروا، سواء كان في ال deletion أو + +573 +00:44:16,550 --> 00:44:19,410 +كان في مين، اللي هو ال insertion، يعني بمعنى آخر + +574 +00:44:19,410 --> 00:44:24,170 +إن كل الحلقات أو كل القواعد اللي بعد الإضافة أو + +575 +00:44:24,170 --> 00:44:28,390 +الحدث، هيتغيروا ال amino acidإذا ال imitation + +576 +00:44:28,390 --> 00:44:33,490 +نوعان، base substitution والتانية frame shift + +577 +00:44:33,490 --> 00:44:36,330 +imitation، وعندها ان شاء الله بنوقف و بنكمل + +578 +00:44:36,330 --> 00:44:37,670 +المحاضرة القادمة ان شاء الله + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/f2aiJbJwTRQ_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/f2aiJbJwTRQ_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..1519102a8c4f290a1dbbf5097f68476f1cd8df57 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/f2aiJbJwTRQ_postprocess.srt @@ -0,0 +1,2548 @@ +1 +00:00:20,690 --> 00:00:24,770 +بسم الله الرحمن الرحيم الموضوع ما زلنا في ال + +2 +00:00:24,770 --> 00:00:29,290 +bacterial infection and immunity وحنعمل quick + +3 +00:00:29,290 --> 00:00:33,410 +revision للما بدأنا بيه المرة الماضية تحدثنا عن + +4 +00:00:33,410 --> 00:00:37,590 +ثلاث مصطلحات متعلقة بوصف العلاقة بين كائنين اللي + +5 +00:00:37,590 --> 00:00:40,550 +هم ال commercialism و الparasitism بالإضافة إلى ال + +6 +00:00:40,550 --> 00:00:44,030 +mutualism و أكدنا على أنه الparasitism هي العلاقة + +7 +00:00:44,030 --> 00:00:44,730 +الأكتر شيء + +8 +00:00:47,840 --> 00:00:51,260 +تحدثنا عن ال normal microbiota و بعض الفوائد + +9 +00:00:51,260 --> 00:00:56,240 +المتنوعة اللي شملناهم تقريبا هم أربع نقاط لكن كنا + +10 +00:00:56,240 --> 00:00:59,780 +عنهم تلاتة ممكن منهم تلاتة أفضل الأولى vitamin K + +11 +00:00:59,780 --> 00:01:03,300 +رقم اتنين ال colonization resistance و تمثل في + +12 +00:01:03,300 --> 00:01:07,420 +شغلتين اللي هو occupying the space and producing + +13 +00:01:07,420 --> 00:01:11,620 +chemicals زي ال bacteriocins و تحدثنا عن ان ال + +14 +00:01:11,620 --> 00:01:14,220 +normal flora قد تساعد في ال development لبعض ال + +15 +00:01:14,220 --> 00:01:17,980 +organs زي ال viral patches اللي عبارة عن مجموعةمن + +16 +00:01:17,980 --> 00:01:22,360 +ال lymph nodes موردة في ال intestinal tract كمان + +17 +00:01:22,360 --> 00:01:25,820 +تطرقنا إلى الفرق ما بين ما يسمى بال hospital + +18 +00:01:25,820 --> 00:01:28,640 +infection وما بين اللي هو ال community acquired + +19 +00:01:28,640 --> 00:01:33,740 +infection وحاولنا ان اكد على مفهوم ان الميكروبات + +20 +00:01:33,740 --> 00:01:38,360 +المكتبة في ال health care institutions are usually + +21 +00:01:38,360 --> 00:01:41,960 +more resistant to antibiotic than those acquired + +22 +00:01:41,960 --> 00:01:46,130 +in the community هذه كانت المفهوم العاموإنه هناك + +23 +00:01:46,130 --> 00:01:49,270 +قد يحدث تغيير في ال normal microbiota ما بين + +24 +00:01:49,270 --> 00:01:53,150 +الموجود في المجتمع وبين الموجود في هذه المؤسسة + +25 +00:01:53,150 --> 00:01:58,570 +وهذه اللوحة كانت تلخيص لهذا الكلام ويبدأ بالحديث + +26 +00:01:58,570 --> 00:02:01,230 +عن مصطلحات مثل pathogen و non pathogen و + +27 +00:02:01,230 --> 00:02:05,650 +opportunistic pathogen و pathogenisticالأن ال + +28 +00:02:05,650 --> 00:02:08,510 +pathogen هو من يمتلك ال pathogenicity وال non + +29 +00:02:08,510 --> 00:02:11,790 +pathogenic هو من لا يمتلك ال pathogenicity ال + +30 +00:02:11,790 --> 00:02:15,070 +opportunistic pathogen هو يمتلك ال pathogenicity + +31 +00:02:15,070 --> 00:02:18,470 +لما يكون الشخص اللي أمامه immunocompromised أما + +32 +00:02:18,470 --> 00:02:23,210 +لما يكون immunocompetent فلا يستطيع أنه يحدث هذا + +33 +00:02:23,210 --> 00:02:29,050 +المرضواتطلقنا الي مصطلح اسمه virulence يقول ان هو + +34 +00:02:29,050 --> 00:02:33,070 +عبارة عن quantitative measure مقياس كم مي لمدى + +35 +00:02:33,070 --> 00:02:37,530 +البعاثة جنسدي و جسدنا اما بال LD50 او ال ID50 + +36 +00:02:37,530 --> 00:02:43,150 +وشرحنا ان ال ID50 هي الجرعة اللازمة لاحداث عدوى + +37 +00:02:43,150 --> 00:02:47,810 +عند 50% من حيوانات التجارب او ال test animal LD50 + +38 +00:02:47,810 --> 00:02:53,950 +بدل ما هي infection بتسبب وفيات او قتلأعتقد أنها + +39 +00:02:53,950 --> 00:03:00,410 +واجهة في نفسي عند اللوحة هذه أحد أهم العناصر اللي + +40 +00:03:00,410 --> 00:03:03,270 +بتساهم في ال virulence حاجة اسمها adherence + +41 +00:03:03,270 --> 00:03:06,550 +ولاحظوا ال adherence أنا معلم على ثلاث كلمات في + +42 +00:03:06,550 --> 00:03:10,050 +اللوحة adherence, adhesion أو attachment كلهم + +43 +00:03:10,050 --> 00:03:14,990 +بيبقى نفس الإحاء المهم ان نفهم ان ال adhesion + +44 +00:03:14,990 --> 00:03:21,130 +غالبا specific ايش ان specific يعني مركب ما مركب + +45 +00:03:32,630 --> 00:03:37,070 +الانفجار هو مفهومه + +46 +00:03:37,070 --> 00:03:41,450 +إن الميكروب قادر على انتقال من مكان الإصابة إلى + +47 +00:03:41,450 --> 00:03:48,030 +أماكن أخرى في غزو في غزو للأنسجة فمن يمتلك انفجار + +48 +00:03:48,030 --> 00:03:51,310 +انفجار يمتلك المقدر على انه ينتقل من مكان إلى مكان + +49 +00:03:51,310 --> 00:03:56,740 +أخرالسلمونيلا طايفي نشربها أو بناكلها مع الغذاء + +50 +00:03:56,740 --> 00:04:02,940 +السلمونيلا طايفي بسبب تايفويد فيبر السلمونيلا + +51 +00:04:02,940 --> 00:04:06,580 +طايفي بتتكاثر في الأمعاء لكن لا تكتفي من مكوثها في + +52 +00:04:06,580 --> 00:04:09,440 +الأمعاء تختارق في دار الأمعاء بتروح على ال lymph + +53 +00:04:09,440 --> 00:04:12,360 +node و من ال lymph node بتروح على ال blood و بتصير + +54 +00:04:12,360 --> 00:04:16,660 +موجودة في الدم و من الدم قد تنتقل إلى ما يسمى + +55 +00:04:16,660 --> 00:04:19,360 +pericardial endothelial system من ضمنها ال liver و + +56 +00:04:19,360 --> 00:04:24,160 +الفي ال bone مرة ممكن و ممكن تصل لل central + +57 +00:04:24,160 --> 00:04:28,760 +nervous system بتعمل هنا meningitis غزوا ولا مش + +58 +00:04:28,760 --> 00:04:34,580 +غزوا؟ غزوا بينما ال eclostridium tetany ال بتاع + +59 +00:04:34,580 --> 00:04:43,590 +مغيتروس بتنغز في مكان محدد و تبقى هناكولا تستطيع + +60 +00:04:43,590 --> 00:04:46,590 +إنها تنتقل من مكان الإصابة إلى مكان أخر، بينما + +61 +00:04:46,590 --> 00:04:52,010 +الـtoxin تبعها هو اللي بينتقل، لكن هي localized، + +62 +00:04:52,010 --> 00:04:57,070 +الأولى systemic، انتشرت الأولى، انتشرت التانية، + +63 +00:04:57,070 --> 00:05:01,770 +localized، وبالتالي الأولى invasive والتانية non + +64 +00:05:01,770 --> 00:05:06,380 +-invasive، non-invasive، كتير مهم المفهوم هذاو ال + +65 +00:05:06,380 --> 00:05:10,920 +adherence و ال invasion يعني معناين مختلفين لكن + +66 +00:05:10,920 --> 00:05:14,500 +كلهم بيصبوا في ال virulence و ال virulence مقياس + +67 +00:05:14,500 --> 00:05:20,000 +للبعض هو innocent المصطلح التالت toxicity و برضه + +68 +00:05:20,000 --> 00:05:24,940 +هذا مصطلح يصب في نفس الإطار بكتيريا تمتلك تكسين و + +69 +00:05:24,940 --> 00:05:30,620 +بكتيريا لا تمتلك تكسين خدوا أبلغوا من شغلهناك بعض + +70 +00:05:30,620 --> 00:05:37,140 +الأمراض الأعراض كلها الناتجة عن المرض بتكون ناتجة + +71 +00:05:37,140 --> 00:05:40,700 +عن التوكسين نفسه زي التيتانوس اللى ذكرنا جاب شوية + +72 +00:05:40,700 --> 00:05:46,760 +البكتيريا اللى موجودة في القدم على سبيل المثال لكن + +73 +00:05:46,760 --> 00:05:50,660 +الأعراض متمثلة بشلل وشلل الاسباستيك بيرانسس + +74 +00:05:53,650 --> 00:05:58,210 +سببه مش الوجود البكتيريا وانما في التوكسين بمعنى + +75 +00:05:58,210 --> 00:06:01,690 +لو أخدنا البكتيريا و ربناها برا و أخدنا ال toxin + +76 +00:06:01,690 --> 00:06:05,390 +تبعها و حقنها في الإنسان فيحدث نفس الأعلى تبع ال + +77 +00:06:05,390 --> 00:06:09,370 +tetanus إذا لما تكون البكتيريا toxigenic تخلف على + +78 +00:06:09,370 --> 00:06:12,790 +ما يكون non-toxigenic بس بودأ أذكركوا يا بنات + +79 +00:06:12,790 --> 00:06:16,430 +بالمفهوم اللي مرض على صناعة لما اتكلمنا عن ال + +80 +00:06:16,430 --> 00:06:21,240 +bacterophageلما اتكلمنا عن ال isogenic cycle لما + +81 +00:06:21,240 --> 00:06:24,960 +اتكلمنا عن ال profage لداخل و لما اتكلمنا عن ال + +82 +00:06:24,960 --> 00:06:29,140 +isogenized cell و ان ان كتير من البكتيريا + +83 +00:06:29,140 --> 00:06:33,860 +التوكسوجينيك اصبحت توكسوجينيك لإن فيها profage، + +84 +00:06:33,860 --> 00:06:37,300 +هذه هي اللي تبقى لنا ذاكرينها السيد روبرت كوخ + +85 +00:06:37,300 --> 00:06:43,360 +للألمان و للكثيرين يعتبر هو أبو ال micro biology + +86 +00:06:43,360 --> 00:06:47,380 +الحديثة طبعاطبعا الفرنسيين بيقولوا لأ Louis + +87 +00:06:47,380 --> 00:06:52,760 +Pasteur لأن هو فرنسي فالتاريخ يكتبه من .. من يكتبه + +88 +00:06:52,760 --> 00:06:57,880 +Robert Koch من إنجازاته الكبيرة الصحيح أنه حاول + +89 +00:06:57,880 --> 00:07:03,380 +يعمل علاقة ما بين ال pathogen و ما بين المرض قال + +90 +00:07:03,380 --> 00:07:07,420 +إذا أردنا أن نثبت أن هذا الميكروب هو المسبب لهذا + +91 +00:07:07,420 --> 00:07:12,470 +المرض فعلينا الإجراءات التاليةالان خدوا بالكم ان + +92 +00:07:12,470 --> 00:07:17,250 +الفرضية كانت صالحة في ذاك الزمن و قاتت أكلها بمعنى + +93 +00:07:17,250 --> 00:07:23,750 +أثبت من خلالها هو و غيره كثير من المسببات للأمراض + +94 +00:07:23,750 --> 00:07:26,890 +فال Vibrio cholera هي المسببة للكوليرا ال + +95 +00:07:26,890 --> 00:07:29,330 +Mycobacterium tuberculosis هي المسبب للكوليرا + +96 +00:07:29,330 --> 00:07:33,830 +الbacillus anthracis هي المسببة للأنترا خلينا + +97 +00:07:33,830 --> 00:07:36,810 +نتكلم مع بعض و نطلع على ال animation + +98 +00:07:39,760 --> 00:07:43,680 +الفرضية الأولى كانت بتقول أنه الميكروب المسبب يجب + +99 +00:07:43,680 --> 00:07:48,140 +أن يكون موجود في الحيوان المصاب و ليس موجودا فيه + +100 +00:07:48,140 --> 00:07:53,180 +الحيوان السليم هذه أول خطوة في الفرضية الخطوة + +101 +00:07:53,180 --> 00:07:57,420 +التانية إذا لازم نعزل الميكروب من الحيوان المصاب + +102 +00:07:57,420 --> 00:08:04,380 +بصورة نقية عشان نعجب صورة نقية نحقنه في الحيوان + +103 +00:08:04,380 --> 00:08:09,230 +السليم يجب أن يظهر عليه نفس الأعراضواجب أن نقوم + +104 +00:08:09,230 --> 00:08:15,810 +بعزل الميكروب مرة أخرى، وصلت الصورة ولا لسه؟ إذا + +105 +00:08:15,810 --> 00:08:19,050 +ماوصلتش، نطلع على الخطوات، ياريت نركز على اللوحة + +106 +00:08:19,050 --> 00:08:24,910 +الآن، الـmicrobe is present in each case of the + +107 +00:08:24,910 --> 00:08:28,810 +disease, but not in healthy animals، أعتقد أن + +108 +00:08:28,810 --> 00:08:32,530 +الصورة الآن اللي يبقى بنا بتوضح أن الفقر المصاب + +109 +00:08:32,530 --> 00:08:35,870 +فيه الميكروب، بينما ال healthy animal ماهندوش + +110 +00:08:35,870 --> 00:08:41,460 +الميكروبالخطوة التانية إن نقوم بعزل ال microbe من + +111 +00:08:41,460 --> 00:08:45,320 +الفقر أو الحيوان اللي هو المصاب in pure culture + +112 +00:08:45,320 --> 00:08:48,420 +إذا + +113 +00:08:48,420 --> 00:08:51,640 +حققنا ال microbe اللي عزلناه من الأول في التاني + +114 +00:08:51,640 --> 00:08:58,440 +السليم يجب أن يحدث عنه نفس اللي هو العربي ويجب أن + +115 +00:08:58,440 --> 00:09:02,800 +نقوم بعزل ال microbe مرة أخرى من ال experimentally + +116 +00:09:02,800 --> 00:09:07,190 +infected اللي هو animalطب أنا قاعد بقول إن الفرضي + +117 +00:09:07,190 --> 00:09:10,730 +في ذلك الزمن قعدت أكلها و اليوم في عليها علامة + +118 +00:09:10,730 --> 00:09:14,870 +استفهام إيش علامة الاستفهام؟ الفرضي الأولى جلد إنه + +119 +00:09:14,870 --> 00:09:18,850 +يجب أن يكون في الحيوان المصاب ولا يزال في السلين + +120 +00:09:18,850 --> 00:09:23,190 +طب أنا بتعطيكم أمثلة كثيرة على مايكروبات موجودة في + +121 +00:09:23,190 --> 00:09:27,510 +المصاب وموجودة في السلين Staphylococcus aureus على + +122 +00:09:27,510 --> 00:09:30,710 +جلدنا موجودة وفي الفم تبعنا وعلى عينينا وعلى .. + +123 +00:09:30,710 --> 00:09:35,040 +يعني أماكن متنوعة من أجسامناو في نفس الوجهة في ناس + +124 +00:09:35,040 --> 00:09:38,900 +مرضى عندهم دمامل و خراجات و حبوب اللى بيطلع منها + +125 +00:09:38,900 --> 00:09:44,480 +الصدر الأبيض Staphylococcus aureus إذا ليس بالضلال + +126 +00:09:44,480 --> 00:09:47,980 +أن يكون ال microbe في ال .. في ال .. يعني ممكن + +127 +00:09:47,980 --> 00:09:50,660 +يكون مورود في التانية في ال .. لإن ال infection + +128 +00:09:50,660 --> 00:09:54,000 +كانت interaction من ثلاث عوامل فاكرينهم؟ ثلاث + +129 +00:09:54,000 --> 00:09:58,440 +عوامل ذكرناهم ال host immunity ال type يبقى في + +130 +00:09:58,440 --> 00:10:01,700 +عامل مهم جدا كان اللى هو ال host immunity مااخدوش + +131 +00:10:01,700 --> 00:10:06,310 +بقى يعني اللى هو الاعتباربدي اعطي مثال تاني بس + +132 +00:10:06,310 --> 00:10:10,090 +للاطمئنان في ميكروب مشهور جدا اسمه نيسيريا + +133 +00:10:10,090 --> 00:10:16,430 +مينينجيتايدس بتسبب التهاب السحية المينينجايتس هذا + +134 +00:10:16,430 --> 00:10:20,530 +موجود ك normal flora او ك normal microbiota في + +135 +00:10:20,530 --> 00:10:25,170 +أنوف اكثر من 15% من الناس اللي صحها يعني لو من 100 + +136 +00:10:25,170 --> 00:10:30,530 +طالبة أخدنا مسحات من الأنف ممكن نازل من 15% منهم + +137 +00:10:30,530 --> 00:10:37,800 +لمهام نيسيريا مينينجيتايدسهذا يضحط اللي هو ال band + +138 +00:10:37,800 --> 00:10:41,620 +الأول في فرضية كوخه إيش كمان في نقاط ضعف في فرضية + +139 +00:10:41,620 --> 00:10:47,320 +كوخه النقطة الثانية هي أنه أصر على أنه لازم نعزل + +140 +00:10:47,320 --> 00:10:52,060 +الميكروب بصورة نقية من الحيوان المصاب أو من حتى ال + +141 +00:10:52,060 --> 00:10:55,700 +experimental التاني طب هناك ميكروبات لحد الآن + +142 +00:10:55,700 --> 00:10:59,820 +ماقدرنا نميها في المختبر زي ال mycobacterium libri + +143 +00:10:59,820 --> 00:11:03,390 +اللي هي بسبب الجذع لحد الآن مالميناهاكتير من + +144 +00:11:03,390 --> 00:11:07,630 +الفيروسات لحد الآن ماقدرنا نميها في المختبر ومع + +145 +00:11:07,630 --> 00:11:11,790 +ذلك أثبتنا أنهي المسؤولة بطلوب أخرى غير اللي هو + +146 +00:11:11,790 --> 00:11:16,110 +فرضيتكوخ إذا فرضيتكوخ في ذاك الزمن كانت صالحة الآن + +147 +00:11:16,110 --> 00:11:18,750 +فيها عيبين أساسيا اللي هم العيب الأول اللي بكرناه + +148 +00:11:18,750 --> 00:11:21,710 +والتاني اللي هو متعلق بعزل ال micro + +149 +00:11:25,900 --> 00:11:31,700 +هذا ليس نقصا او انقاصا من حقوق Robert Koch بالعكس + +150 +00:11:31,700 --> 00:11:34,640 +يعني هو كان زي ما قلتلكوا انه من العلماء اللي .. + +151 +00:11:34,640 --> 00:11:38,200 +يعني اللي .. اللي سهموا وسامد عظيمة في وضع الصورة + +152 +00:11:38,200 --> 00:11:41,920 +نفس الفكرة اللي أمامكوا نفس الصورة اللي احنا + +153 +00:11:41,920 --> 00:11:46,610 +عرضناها في ال animationاللوحة هذه التالت اللي + +154 +00:11:46,610 --> 00:11:49,350 +موجودة فيها تلت دوائر اللي هم ال interaction ما + +155 +00:11:49,350 --> 00:11:54,010 +بين ال immune system تبع ال host و الجرعة و اللي + +156 +00:11:54,010 --> 00:11:57,610 +هو ال strain اللي جونا ال type و أنا ذكرت المحاضرة + +157 +00:11:57,610 --> 00:12:03,470 +السابقة قولت شجرة نحتاج الى واحد لمية microbe + +158 +00:12:03,470 --> 00:12:06,510 +تقريبا عشان نعمل infection عند الإنسان بينما ال + +159 +00:12:06,510 --> 00:12:10,670 +virus اللي جونا نحتاج الى مليون من واحد لمية يعني + +160 +00:12:10,670 --> 00:12:14,800 +ال number قليلو مليون number عالى يعني لو بدنا + +161 +00:12:14,800 --> 00:12:17,880 +نحطهم في الميزان تبع ال virulence بيقول مين is + +162 +00:12:17,880 --> 00:12:22,060 +more virulent الشجلة اللي هم عددها طبعا أقل اللي + +163 +00:12:22,060 --> 00:12:27,020 +هي عددها أقل كمان مرة ما ننساش التلات عوامل اللي + +164 +00:12:27,020 --> 00:12:30,020 +موجودين على اللوحة أمامنا اللي هو ال immune system + +165 +00:12:30,020 --> 00:12:33,620 +ال strength زي اللي هو ال virulence و أنا أضفت لهم + +166 +00:12:33,620 --> 00:12:38,520 +بُعد رابع المرة الماضية مين بيذكر البُعد الرابع ال + +167 +00:12:38,520 --> 00:12:43,370 +portal of entryمكان الدخول و ذكرتلكوا في المحاضرة + +168 +00:12:43,370 --> 00:12:47,430 +انه مثال ال .. ال .. الbacillus anthracis لو دخل + +169 +00:12:47,430 --> 00:12:51,110 +عبر الجلد او دخل عبر ال lungs او دخل عبر ال + +170 +00:12:51,110 --> 00:12:54,630 +gastrointestinal الأرقام اختلفت تماما اذا ال + +171 +00:12:54,630 --> 00:12:58,550 +portal of entry كمان كان بارد رابعا الصورة اللي + +172 +00:12:58,550 --> 00:13:03,810 +أمامكم بتتكلم عنbacterial virus factors انا لما + +173 +00:13:03,810 --> 00:13:07,710 +دراستكوا ال bacterial cell structure قولتلكوا + +174 +00:13:07,710 --> 00:13:13,470 +بناتنا الكلمات انه لسنا بصدق انه نحفظ بس التراكيب + +175 +00:13:13,470 --> 00:13:16,310 +انه التراكيب هدس ال wall هدس ال membrane و اين + +176 +00:13:16,310 --> 00:13:21,470 +ماكان اقولت لأ احنا بدنا نفهم ما ورا التراكيب و + +177 +00:13:21,470 --> 00:13:26,030 +كنا كتير عن التراكيب اعطيكم مثال بسيط جدا وسهل كنا + +178 +00:13:26,030 --> 00:13:30,510 +نقول عن ال flagella سمينها virus factor لانه بتهرب + +179 +00:13:30,510 --> 00:13:34,430 +من خطروبتذهب إلى غذاء، بتذهب إلى غذاء من خلال + +180 +00:13:34,430 --> 00:13:38,710 +الحركة أو تهرب من خطر في حال ان اتعرض هذا الخطر، + +181 +00:13:38,710 --> 00:13:42,570 +هذا اعتبرنا احنا by an instinct لما اتكلمنا على ال + +182 +00:13:42,570 --> 00:13:46,190 +attachment تحدثنا ايضا على ان ال attachment في + +183 +00:13:46,190 --> 00:13:50,770 +اناكن محددة ضرورة من ضرورات البقاء و ال + +184 +00:13:50,770 --> 00:13:53,830 +colonization تمام و بالتالي infection و بالتالي + +185 +00:13:53,830 --> 00:13:58,060 +diseaseيبقى كل التراكيب اللي درسناهم ما درسناهم + +186 +00:13:58,060 --> 00:14:04,320 +هكذا هكذا ان الان موعدهم ان احنا نعطيهم حقهم في ال + +187 +00:14:04,320 --> 00:14:09,780 +ال ال ال virans طيب + +188 +00:14:09,780 --> 00:14:13,980 +ماعرفش الجملة ماعرفش ليش مش واضحة في نقاط مش هتنزل + +189 +00:14:13,980 --> 00:14:18,660 +على اللوحة لكن بنقولكوا عنها regulation of + +190 +00:14:18,660 --> 00:14:23,350 +bacterial virance factorsبتير بيخطر على بال ان + +191 +00:14:23,350 --> 00:14:29,650 +احنا كبشر ان احنا كنا عند نفس المريض و تقريبا كنا + +192 +00:14:29,650 --> 00:14:33,330 +بنفس الدرجة من القرب من هذا المريض انا أصيبت + +193 +00:14:33,330 --> 00:14:37,430 +بالمرض و زميل لم يصاب لماذا انا اصيبت و هو لم يصاب + +194 +00:14:37,430 --> 00:14:42,330 +او العكس فتفسيراتنا بتقعد .. بنقعد .. بنحاول .. + +195 +00:14:42,330 --> 00:14:46,210 +نجتهد ان انت كنت اقرب اكتر او هو كان اقرب .. طب + +196 +00:14:46,210 --> 00:14:50,410 +هناك عوامل كثيرة احنا درست 3 عوامل قبل شوية منهم و + +197 +00:14:50,410 --> 00:14:55,060 +عملنا بواد رابع كنا منهماللي هو ال portal of + +198 +00:14:55,060 --> 00:15:00,560 +ending طيب ال strain .. ال strain عشان يعمل ال + +199 +00:15:00,560 --> 00:15:06,620 +virulenceفي حاجة بيسموها إما triggering، + +200 +00:15:06,620 --> 00:15:11,280 +triggering يعني إطلاق أو inciting حث، inciting + +201 +00:15:11,280 --> 00:15:14,440 +factors، triggering factors أو trigger اللي هو ضغط + +202 +00:15:14,440 --> 00:15:18,240 +الزناد يعني، تضغط على الزناد ليش؟ تضغط على الزناد + +203 +00:15:18,240 --> 00:15:22,060 +to produce the virulence factor و أحد أهمها + +204 +00:15:22,060 --> 00:15:24,520 +شرحتلكم المرة الماضية أنا حاسمها quorum sensing + +205 +00:15:24,520 --> 00:15:28,020 +فاكرينها؟ quorum sensing لما قلنا أنه عدد + +206 +00:15:28,020 --> 00:15:33,080 +البكتيريا، البكتيريا عندها المقدرة تشهرهو إذا + +207 +00:15:33,080 --> 00:15:36,760 +استشعت إن عددها صار مؤهل أن تنتج تكسين تنتج + +208 +00:15:36,760 --> 00:15:40,800 +التكسين هو ال virulence مش هو virus factor تكسين + +209 +00:15:40,800 --> 00:15:44,280 +طب إيش العوامل الأخرى اللي ممكن تأثر على إنتاج ال + +210 +00:15:44,280 --> 00:15:47,340 +virulence factors؟ أنا بقولوا أحدها ال + +211 +00:15:47,340 --> 00:15:54,640 +temperature، ال pH، ممكن ال osmolarity، ممكن ال + +212 +00:15:54,640 --> 00:16:01,060 +iron، ال iron يعني الحديدبعض ال ions المحددة إذا + +213 +00:16:01,060 --> 00:16:05,620 +ولد أو لم توجد كلها ممكن تساهم في switch on و + +214 +00:16:05,620 --> 00:16:10,780 +switch off يبقى + +215 +00:16:10,780 --> 00:16:16,140 +الآن لما بنيجي نفسر لماذا فلان أصيب و فلان لم يصاب + +216 +00:16:16,140 --> 00:16:19,800 +الأعمالية ازدادت تعقيدا العمالية ازدادت تعقيدا كيف + +217 +00:16:19,800 --> 00:16:23,980 +ازدادت تعقيدا احنا كنا متكلمين على تلات عوامل host + +218 +00:16:23,980 --> 00:16:30,500 +immunityو strain وش كمان؟ و الدوس طبعا طيب احنا + +219 +00:16:30,500 --> 00:16:34,200 +اتعرضنا لنفس ال strain و اتعرضنا لنفس الدوس على + +220 +00:16:34,200 --> 00:16:36,440 +سبيل المثال مين ضال الآن التالت؟ + +221 +00:16:40,870 --> 00:16:44,970 +طيب احنا ال immunity تبعتنا كانت determined عامل + +222 +00:16:44,970 --> 00:16:48,830 +محدد عامل محدد طب في كمان عامل محدد احنا اتنين + +223 +00:16:48,830 --> 00:16:52,930 +افترقنا عن بعض احنا اتنين افترقنا عن بعض انا تعرضت + +224 +00:16:52,930 --> 00:16:57,950 +لظروف و انت تعرضت لظروف اخرى قد تساهم في switching + +225 +00:16:57,950 --> 00:17:01,470 +on او switching off لمين لبعض ال virulence factors + +226 +00:17:01,470 --> 00:17:05,930 +اذا خلينا نقول من خلال معرفتنا بان في regulation + +227 +00:17:07,100 --> 00:17:10,120 +الانتاج تبقى ال violence factor مش دائما هو نفس + +228 +00:17:10,120 --> 00:17:15,340 +الانتاج بيعتمد على ظروف معينة بيئية وغذائية يبقى + +229 +00:17:15,340 --> 00:17:18,500 +معنا الحكي بنقدر نفسر لماذا هناك اختلافات في نسبة + +230 +00:17:18,500 --> 00:17:22,320 +الإصابة بين مجموعة من البشر اتعرضوا لنفس اللي ايه؟ + +231 +00:17:22,320 --> 00:17:26,280 +نفس الأوان اول + +232 +00:17:26,280 --> 00:17:31,940 +اولنقطة بنركز عليها اليوم في ال virulence factor + +233 +00:17:31,940 --> 00:17:34,860 +اللي هو ال adherence factor اللي يقول عنهم + +234 +00:17:34,860 --> 00:17:40,220 +adherence, adhesion او attachment اتولد صغير كتير + +235 +00:17:40,220 --> 00:17:44,600 +خالص اه يعني غير مرئي حتى بالنسبالي انا هتطلع ألبس + +236 +00:17:44,600 --> 00:17:52,250 +المضارة بدأ اقرأ بس بالأول أسماءللمراكبات اللي + +237 +00:17:52,250 --> 00:17:56,410 +كلها اعتبرناها adherence factors adhesin, + +238 +00:17:57,010 --> 00:18:02,710 +receptor, lectin, ligand, mucus, fimbria, common + +239 +00:18:02,710 --> 00:18:08,050 +pili, six pilas، اللي هي six pili يعني، pili جمع، + +240 +00:18:08,050 --> 00:18:12,710 +pilas اللي هي singular، type 1 fimbria، type 4 + +241 +00:18:12,710 --> 00:18:18,950 +pili, S layerالـ S Layer مرة عليكم Slime Layer و S + +242 +00:18:18,950 --> 00:18:22,010 +Layer و Capsule و Micro Capsule الجليكوكاليكس + +243 +00:18:22,010 --> 00:18:26,210 +الاسم العام لكلهم Capsule Lipopolysaccharide + +244 +00:18:26,210 --> 00:18:33,410 +Tychoic Acid and Lipotychoic Acid كل ما بكرناه هي + +245 +00:18:33,410 --> 00:18:37,510 +تراكيب موجودة في بعض أنواع من البكتيريا و غير + +246 +00:18:37,510 --> 00:18:43,410 +موجودة في بعض أنواع أخرى مع ده رقم اتنين اليمين ال + +247 +00:18:43,410 --> 00:18:49,570 +receptor ال receptorبنطلقه على التركيب اللي موجود + +248 +00:18:49,570 --> 00:18:52,270 +في خلايا ال E-Eukaryotes اللي هم ال host اللي هم + +249 +00:18:52,270 --> 00:18:56,030 +أحلى تمام اللي ممكن يرتبط فيها واحد من هذه + +250 +00:18:56,030 --> 00:19:01,390 +المركبات اللي هو البكتيروس الصورة بمعنى أخر أنه + +251 +00:19:01,390 --> 00:19:06,130 +مثلا الأدهسين بدهو receptor الليكتين بدهو receptor + +252 +00:19:06,130 --> 00:19:09,170 +الليجان بدهو receptor يبقى ال receptor هو المركب + +253 +00:19:09,170 --> 00:19:14,790 +المستقبل طيب في نقطة كتير مهمة أذكركوا و أرجع بيكم + +254 +00:19:14,790 --> 00:19:20,250 +بالبكتيروفاجالبكتيروفاج عمل infection لمن؟ + +255 +00:19:20,250 --> 00:19:25,090 +للبكتيريا لم يستطيع ان يعمل infection لا للنباتات + +256 +00:19:25,090 --> 00:19:31,730 +ولا للحيوانات تمام؟ الان بدنا .. برضه بدنا نعكس + +257 +00:19:31,730 --> 00:19:36,350 +الموضوع الفيروس اللى بيصيبنا احنا تمام؟ احنا + +258 +00:19:36,350 --> 00:19:39,670 +بيسموه animal viruses او human viruses تمام؟ ما + +259 +00:19:39,670 --> 00:19:43,270 +بتصيب النباتات؟ ما بتصيب النباتات؟ لإن مافيش اللي + +260 +00:19:43,270 --> 00:19:49,590 +هي receptors للنباتاتو هذا يسمى host specificity + +261 +00:19:49,590 --> 00:19:56,070 +يعني في نوع من التخصص ما بين ال microbe المعني و + +262 +00:19:56,070 --> 00:20:01,930 +ما بين ال host او بالظبط ال host يعني بصيب البط و + +263 +00:20:01,930 --> 00:20:05,730 +بصيب الحمام لكن مابصيبش الخنازير على سبيل المثال + +264 +00:20:05,730 --> 00:20:10,090 +او بصيب الخنازير و الخيل و ايك البط لكن مابصيبش + +265 +00:20:10,090 --> 00:20:14,990 +الحماممؤشر عليا على انه لا يحتوي ال receptor في + +266 +00:20:14,990 --> 00:20:17,290 +الحيوانات وهذا الكلام على فكرة بنشوفه في ال + +267 +00:20:17,290 --> 00:20:21,630 +influenza بنلاقي ال influenza بعض .. بعض السلالات + +268 +00:20:21,630 --> 00:20:26,930 +منها بتصيب الطيور وبعض السلالات بتصيب مثلا الخيل + +269 +00:20:26,930 --> 00:20:32,270 +البعض بتصيب الخنازير والبعض بتصيب خد بالك قد يحدث + +270 +00:20:32,270 --> 00:20:37,400 +ان يكون فيعامل مشترك مثلا اللي أجي فيروس influenza + +271 +00:20:37,400 --> 00:20:42,260 +بيصيب الخيل و الخنزير و واحد بيصيب الإنسان و + +272 +00:20:42,260 --> 00:20:45,440 +الخنزير لكن مابيصيبش الإنسان و الخيل في نفس الوقت، + +273 +00:20:45,440 --> 00:20:49,160 +مين العامل المشترك اللي اهان؟ الخنزير أكرمكم الله، + +274 +00:20:49,160 --> 00:20:53,470 +و هذا اللي بيحدث عندها تغيير تفرادإنه سلالتين + +275 +00:20:53,470 --> 00:20:56,790 +واحدة من الإنسان و واحدة من الخيل بيعملوا ال co + +276 +00:20:56,790 --> 00:21:00,630 +-infection في نفس اللحظة في الخنزير فقد يحدث تبادل + +277 +00:21:00,630 --> 00:21:05,610 +للجينات باسمها gene reassortment هدرسها لاحقا و ال + +278 +00:21:05,610 --> 00:21:09,030 +reassortment هدى عبارة عن استبدال قطع من المادة + +279 +00:21:09,030 --> 00:21:13,190 +الوراثية من كل من ال ال virusين و بيطلعنا سلالة + +280 +00:21:13,190 --> 00:21:19,690 +جديدة سلالة جديدةيبقى معنا الحكي انه بنفهم انه + +281 +00:21:19,690 --> 00:21:25,610 +عملية ال .. ال .. ال .. ال specificity تبع ال + +282 +00:21:25,610 --> 00:21:29,210 +receptor مرتبطة .. او افر ال .. ال .. ال .. ال + +283 +00:21:29,210 --> 00:21:33,090 +infection مرتبطة بوجود receptor للعوامل تبع ال + +284 +00:21:33,090 --> 00:21:36,310 +attachment تبع ال bacteria او اللي هو الفيروسة + +285 +00:21:36,310 --> 00:21:40,370 +واحنا بنقولكوا كمان مرة دائما و ابدا اول خطوة في + +286 +00:21:40,370 --> 00:21:42,670 +ال infection غالبا هي ال attachment + +287 +00:21:45,700 --> 00:21:48,780 +أنا قلت عن مين؟ عن ال species أو ال host + +288 +00:21:48,780 --> 00:21:53,220 +specificity، صح؟ طب داخل ال host الواحد، احنا زي + +289 +00:21:53,220 --> 00:21:57,980 +ما مثلا إنسان بشر، تراكيب الخلايا تبعته و الأنسجة + +290 +00:21:57,980 --> 00:22:01,280 +و الأعضاء مختلفة تماما، ال cardiac muscle غير عن + +291 +00:22:01,280 --> 00:22:03,280 +ال intestinal، الepithelium اللي موجود في + +292 +00:22:03,280 --> 00:22:06,420 +الانتستين غير عن الepithelium اللي موجود في ال + +293 +00:22:06,420 --> 00:22:11,670 +lungs، فهل هناكهل يكون هناك مجتمع عالمي على كل + +294 +00:22:11,670 --> 00:22:16,230 +الأنسجة؟ يمكن لأ ويمكن أ لأن في بعض المركبات لازم + +295 +00:22:16,230 --> 00:22:21,310 +تكون مشتركة بين كل الخلايا مثلًا مجتمع لا للوكوز + +296 +00:22:21,310 --> 00:22:22,070 +على سبيل المثال + +297 +00:22:25,800 --> 00:22:29,080 +إذا ماكانش على كل الأنثى لهذا الريسبتور اللى + +298 +00:22:29,080 --> 00:22:32,280 +بتستخدمه البتيرة في الإرتباط فمع ذلك الحكي إن هذا + +299 +00:22:32,280 --> 00:22:35,360 +الريسبتور هو اللى بيحدد ال organ أو ال tissue + +300 +00:22:35,360 --> 00:22:39,320 +tropism أو ال specificity كلمة tropism يعني ال + +301 +00:22:39,320 --> 00:22:43,180 +affinity affinity يعني القابلية للإرتباط، تمام؟ + +302 +00:22:43,180 --> 00:22:46,340 +ففي عندى شغلتين أنثى عندنا من ال adherence واحدة + +303 +00:22:46,340 --> 00:22:51,900 +متعلقة بال species specificity وتانية من dual + +304 +00:22:51,900 --> 00:22:55,000 +species اللى هو تيشيو تروبزم + +305 +00:22:58,370 --> 00:23:01,910 +طيب الجدول هذا اللي قدامكوا بنطلع عليه كويس لإن هو + +306 +00:23:01,910 --> 00:23:08,130 +اللي بوضح الفكرة تماما بتقرأ أسماء البكتيريا اللي + +307 +00:23:08,130 --> 00:23:15,370 +في أقصى اليسار و بعدين أطلع على العمود الأخير عشان + +308 +00:23:15,370 --> 00:23:20,430 +أوضح نقطة Striptococcus biogenes هذه رفيقة الدرب + +309 +00:23:20,430 --> 00:23:25,270 +تبعتكمتذكروا دائما انه يوم ما تنزل اللواز تصير + +310 +00:23:25,270 --> 00:23:28,710 +عندك الواز يعني انتهاد اللواز هيعرف انه هدى + +311 +00:23:28,710 --> 00:23:32,990 +streptococcus pyruids streptococcus mutants و ال + +312 +00:23:32,990 --> 00:23:36,590 +streptococcus salivaris هدولا تنتين موجودين في + +313 +00:23:36,590 --> 00:23:40,790 +اللعاب في القلعة الكبيرة ك normal flora ك normal + +314 +00:23:40,790 --> 00:23:46,970 +flora لكن ممكن أحداهم اللى هى الأولى ال mutants هي + +315 +00:23:46,970 --> 00:23:49,370 +تعمل dental caries تسوص الأصناف + +316 +00:23:52,850 --> 00:23:56,390 +يعني قاصد أن نتكلم على الأسماء بسرعة عشان نحنا + +317 +00:23:56,390 --> 00:24:00,230 +نشوف الأمراض ونشوف أماكن الأمراض هذه وإيش علاقة + +318 +00:24:00,230 --> 00:24:03,930 +الأدات بين الأمراض بالظبط هذي استفدت كوكاس + +319 +00:24:03,930 --> 00:24:08,310 +نيمونيا؟ أين موجودة؟ نيمونيا .. نيمونيا .. في + +320 +00:24:08,310 --> 00:24:11,970 +الرياض تاني، تمام استفدت كوكاس أوريا؟ هذه في كل + +321 +00:24:11,970 --> 00:24:16,950 +مكان، في كل مكان، تمام؟ إن سيريا جنورية؟ مرض جنسي + +322 +00:24:16,950 --> 00:24:21,850 +في الـUrogenital، في الـUrogenital tract طيباللوحة + +323 +00:24:21,850 --> 00:24:24,870 +اللي بعدها enterotoxinic escherichia coli في ال + +324 +00:24:24,870 --> 00:24:28,430 +intestine اللي بتعمل ديارية ال uropathionic + +325 +00:24:28,430 --> 00:24:31,510 +escherichia coli ممكن أنا مرتين لغرضين لكن واحدة + +326 +00:24:31,510 --> 00:24:34,170 +بتعمل urethritis واحدة بتعمل pionifritis وين ال + +327 +00:24:34,170 --> 00:24:37,610 +pionifritis؟ في الكيلية الكيلية بتنزل ال urine + +328 +00:24:37,610 --> 00:24:41,710 +تمام؟ فيه باستمرار washing ال urethritis ال + +329 +00:24:41,710 --> 00:24:45,030 +urethra اللي هي العضو الأخير في ال urinary tract + +330 +00:24:45,030 --> 00:24:49,880 +اللي بعد اللي هو ال bloodوبرضه الأمور عبره مين؟ ال + +331 +00:24:49,880 --> 00:24:53,940 +ball و ال ball بيعمل عملية washing تمام؟ ال + +332 +00:24:53,940 --> 00:24:57,720 +whopping cough في ال .. في ال lungs تمام؟ في + +333 +00:24:57,720 --> 00:25:00,220 +التراكيا اللي هي السعالة الديكي السعالة الديكي + +334 +00:25:00,220 --> 00:25:03,700 +اللي بسببها بوردوتيلا و Pertosis Vipariculera + +335 +00:25:03,700 --> 00:25:08,000 +دياريا tryponema pallidum syphilis مرض جنسي + +336 +00:25:08,000 --> 00:25:12,840 +Mycoplasma ليمونيا في ال lungs Klamydia + +337 +00:25:12,840 --> 00:25:19,320 +Conductivitis التعب المتحمة تبع تلعينor urethritis + +338 +00:25:19,320 --> 00:25:22,560 +زي ال extension كلها الأولانية، طب تعالوا نطلع على + +339 +00:25:22,560 --> 00:25:27,260 +الأماكن اللي احنا ذكرناها sore throat احنا بكونا + +340 +00:25:27,260 --> 00:25:33,760 +نلوازم لكن بنصومش، بمعنى بناكل و بنشرب، طب البلع + +341 +00:25:33,760 --> 00:25:37,500 +مش عملية ضغط على ال tonsils، هناك مش عارفين مش + +342 +00:25:37,500 --> 00:25:42,060 +عارفين القلم شديدمش الميكروبات المفروض إذا ماعنداش + +343 +00:25:42,060 --> 00:25:45,000 +المقدر على ال attachment وموجودة على ال .. تنزل + +344 +00:25:45,000 --> 00:25:48,800 +فيها تروح مع الأكل و تنزل على .. على المعنى و تموت + +345 +00:25:48,800 --> 00:25:52,400 +في المعنىلكن واقع الأمر هناك أنواع من الـ + +346 +00:25:52,400 --> 00:25:55,380 +tonsillitis لما بنطلّع عليها بنفتح الفم و بنشوفها + +347 +00:25:55,380 --> 00:25:59,920 +بنلاقي بُقع بيضاء على الـ tonsil نفسها عارفين شو + +348 +00:25:59,920 --> 00:26:03,280 +البُقع البيضاء هذي؟ مستعمرات striptococcus virus + +349 +00:26:03,280 --> 00:26:06,560 +مستعمرات دلالة على أن البكتيريا قادرة على ال + +350 +00:26:06,560 --> 00:26:11,240 +attach بشكل قوي جدا في هذا المكان طب نيجي لل + +351 +00:26:11,240 --> 00:26:16,400 +pneumonia احنا أول حارب نعملها لما بصيرنا + +352 +00:26:16,400 --> 00:26:20,220 +pneumonia بصير ننكحمظبوط؟ طب والكحة عبارة على + +353 +00:26:20,220 --> 00:26:24,660 +فعلين عن إيش؟ عن explosion من الهواء بيضغط .. + +354 +00:26:24,660 --> 00:26:27,740 +بيضغط .. بيضغط .. بيضغط فيه أمبوبرا فيها + +355 +00:26:27,740 --> 00:26:31,380 +الواتراكيا، يعني المفروض أن أي بكتيريا مع ال .. مع + +356 +00:26:31,380 --> 00:26:36,920 +القدائف إيش يحصل فيها، تطلع برا، ومع ذلك عند + +357 +00:26:36,920 --> 00:26:40,100 +المقدرة على attachment بتبقى ملازقة جنوبهاطب + +358 +00:26:40,100 --> 00:26:43,500 +عارفين التراكيا فيها ما يسمى بالـ Celia Reactions + +359 +00:26:43,500 --> 00:26:47,900 +Celia Reactions اللي هي حركة السيليا الأهداب حركة + +360 +00:26:47,900 --> 00:26:51,880 +السيليا هذه موجودة باستمرار 24 ساعة شغالة و + +361 +00:26:51,880 --> 00:26:56,180 +بيسموها المصعد ايش هي المصعد؟ أسانسير بنزل .. + +362 +00:26:56,180 --> 00:27:00,080 +بيجيب من تحت طلع وين؟ طلع فور إيش بيطلع؟ طلع + +363 +00:27:00,080 --> 00:27:05,320 +الميوكاس، المخاطب جسمنا أو ال lungs بتنتج ميوكاس + +364 +00:27:05,320 --> 00:27:07,900 +عشان تعمل treatment للغبرة و ال micro organism + +365 +00:27:09,630 --> 00:27:16,710 +فباستمرار ال lungs بتنتج ميوكاس بيرتبط بالغبار + +366 +00:27:16,710 --> 00:27:20,430 +و بال micro organism عشان ما يستقرش ال micro + +367 +00:27:20,430 --> 00:27:24,650 +organism بنطلع بره و بنتخلص منه عبر الكحة الخفيفة + +368 +00:27:24,650 --> 00:27:29,630 +في معظم الأحيان تخيلوا ان السيليا اللي بتتحرك هذه + +369 +00:27:29,630 --> 00:27:32,910 +الحركة المنتضمة فجأة واحد قد مسك السيليا هيك + +370 +00:27:39,870 --> 00:27:43,610 +إنه في بكتيريا بتجري تعمل attachment على السيريا، + +371 +00:27:43,610 --> 00:27:48,630 +تمام؟ فبصير في وزن وارتباط عدم انتظام في الحركة + +372 +00:27:48,630 --> 00:27:51,870 +فبصير في عندي accumulation في الbertosis في ال .. + +373 +00:27:51,870 --> 00:27:55,910 +اللي السعالة تديكي accumulation للبكتيريا و ال + +374 +00:27:55,910 --> 00:28:00,150 +mucus في منطقة محددة فبصير في عندي عملية السعالة + +375 +00:28:00,150 --> 00:28:03,530 +اللي هو اللي مستمر لفترة طويلة اللي بيقولنا عنه مش + +376 +00:28:03,530 --> 00:28:07,660 +بسعالةطيب الدياريا مش هنقول نشوفنا + +377 +00:28:07,660 --> 00:28:11,280 +انترولوكتكسوجينك إشريشة كلها و نشوفنا الفيبلو كلها + +378 +00:28:11,280 --> 00:28:14,960 +بتعمل إسهال و الإسهال عبارة عن برضه running water + +379 +00:28:14,960 --> 00:28:18,240 +running water مياه جارية مش مفروض تعمل flushing + +380 +00:28:18,240 --> 00:28:24,340 +للبكتيريا و مع ذلك البكتيريا تبقى هناك تتجمد + +381 +00:28:24,340 --> 00:28:27,640 +تعالوا نطلعوا على اللي بتسبب urinary tract أو + +382 +00:28:27,640 --> 00:28:30,720 +genitourinary tract طب ما ال urine يقولنا احنا + +383 +00:28:30,720 --> 00:28:35,010 +برضه عملية تنظيفومع ذلك البكتيريا تبقى هناك اذا + +384 +00:28:35,010 --> 00:28:39,470 +بدنا نطلع دعاين من الاحترام والتقدير على المركبات + +385 +00:28:39,470 --> 00:28:43,550 +اللي بتخلى البكتيريا تبقى في هذه الأماكن لإنها + +386 +00:28:43,550 --> 00:28:49,710 +المسؤولة طيب أعطيكم مثال على بي لاي اللي موجودة في + +387 +00:28:49,710 --> 00:28:57,990 +نشيليا جونورية وراحت أخر واحدة في القائمة الان + +388 +00:28:57,990 --> 00:29:04,040 +هذه البكتيريا هذه البكتيريارقم واحد فيها virulence + +389 +00:29:04,040 --> 00:29:08,260 +factor هو ال P-Ly هو ال P-Ly طب تخيلوا جسم الإنسان + +390 +00:29:08,260 --> 00:29:11,340 +هو ذكي برضه ال immune system تبقى على ذكي بيروح + +391 +00:29:11,340 --> 00:29:16,500 +بيكوّن antibody لمين؟ لل P-Ly عشان ترجعله وعارف + +392 +00:29:16,500 --> 00:29:20,400 +حالها بدون P-Ly عارف حالها بدون P-Ly غير قادرة على + +393 +00:29:20,400 --> 00:29:24,060 +إنها تبقى في هذا المكان فبتروح تعمل mutation في + +394 +00:29:24,060 --> 00:29:27,520 +مين؟ في ال P-Ly في الروتينة وبالتالي ترجع مرة + +395 +00:29:27,520 --> 00:29:32,780 +تاكتروصل الصورة يعني في ال attachment ثبت بالدليل + +396 +00:29:32,780 --> 00:29:36,980 +القاطع من اللوحة اللي قدامنا على الرغم من اختلاف + +397 +00:29:36,980 --> 00:29:41,200 +المركبات المستخدمة في ال attachment الا انه ال + +398 +00:29:41,200 --> 00:29:45,600 +attachment كغراب هي عامل مهم جدا في عاملته اللي هو + +399 +00:29:45,600 --> 00:29:49,700 +ال path Genesis وبالتالي هي viral as fuck وانا + +400 +00:29:49,700 --> 00:29:56,610 +اتفقنا انه اي عاملبيأثر إيجابا على ال pathogenesis + +401 +00:29:56,610 --> 00:30:03,810 +تبع البكتيريا تمام هو violence factor هذه الفكرة + +402 +00:30:03,810 --> 00:30:08,110 +العامة على ال addition أنه البكتيريا من خلال + +403 +00:30:08,110 --> 00:30:11,530 +الادهسين اللي أين من الأشياء الأخرى اللي ذكرناها + +404 +00:30:11,530 --> 00:30:15,250 +اسمه ادهسين بيرتبط على ال receptor اللي مولد في ال + +405 +00:30:15,250 --> 00:30:19,240 +epithelial cell سبعة أنسجات الإنسانمثل ال + +406 +00:30:19,240 --> 00:30:22,400 +Astrococcus biogenes كانت حاسمة protein F أو F + +407 +00:30:22,400 --> 00:30:26,240 +protein وبيرتبط مع الفيبرونكتين اللي موجود على بعض + +408 +00:30:26,240 --> 00:30:29,300 +ال epithelial cells و هذا ال binding قولنا binding + +409 +00:30:29,300 --> 00:30:33,960 +قوي و من خلاله البكتيريا تبقى هناك على الرغم من + +410 +00:30:33,960 --> 00:30:41,520 +انه في forces بتحاول انها تخلص منكالفيمبريا انا + +411 +00:30:41,520 --> 00:30:45,820 +ممكن في ال structure حاولت اني امر مرور سريع على + +412 +00:30:45,820 --> 00:30:49,220 +وظيفتين رئيسيتين في الفيمبريا او البيلاي و احنا + +413 +00:30:49,220 --> 00:30:51,660 +كنا الفيمبريا و البيلاي كانوا اسمين بيستخدموه + +414 +00:30:51,660 --> 00:30:55,400 +interchangeably بمعناه انهم بي .. مرات بيميزوش + +415 +00:30:55,400 --> 00:30:59,380 +بينهم أساسا لكن خصصوا ال .. البيلاي ال .. يسموها + +416 +00:30:59,380 --> 00:31:02,520 +ال six-pili و التانية جالو عنها attachment فيمبريا + +417 +00:31:02,520 --> 00:31:06,180 +لكن بواقع الأمر لحد دلان كتير من ال textbook + +418 +00:31:06,180 --> 00:31:10,200 +بتتكلم عنهم كانهم تركيب واحداللي انا بدأ أقوله + +419 +00:31:10,200 --> 00:31:13,760 +اليوم مختلف عن اللي قلته في السابق مش لإنه + +420 +00:31:13,760 --> 00:31:17,940 +حكيتلكوا علامات خطأ لأ ماحبناش نعمل confusion في + +421 +00:31:17,940 --> 00:31:21,660 +البداية بس الآن لازم نقولكوا الحقيقة المرة إنه + +422 +00:31:21,660 --> 00:31:26,780 +البيلاي و الفيمبريا ليست بهذه البساطة اللتي + +423 +00:31:26,780 --> 00:31:32,040 +درسناها يعني بمعنى أخر ممكن تلاقوا textbook كامل + +424 +00:31:32,040 --> 00:31:39,550 +عن البيلاي و الفيمبريابيشمل مؤتمرات مظلمة فقط + +425 +00:31:39,550 --> 00:31:43,990 +لدراسة البيلاي والفملي طبعا للي أكيد لهميتها و أنا + +426 +00:31:43,990 --> 00:31:47,810 +قاعد بتكلم إلي ربع ساعة عن ال attachment و أهمية + +427 +00:31:47,810 --> 00:31:51,070 +ال attachment فيه مش بس في ال .. في ال .. في ال + +428 +00:31:51,070 --> 00:31:54,630 +pathogenesis تخيلوا في المستشفيات البكتيرا اللي + +429 +00:31:54,630 --> 00:31:58,170 +عندها قدرة على ال attachment مع الأسطح قد تكون + +430 +00:31:58,170 --> 00:32:03,230 +يسمى biofilms إيش يعني bio؟ films طبقات بيولوجية + +431 +00:32:04,040 --> 00:32:07,140 +طيب أقولكوا إيش يعني biofilm عشان تقدروا الموضوع + +432 +00:32:07,140 --> 00:32:09,360 +ال attachment طبعا ال biofilm كلكوا شفتهوا في + +433 +00:32:09,360 --> 00:32:13,780 +الدار لما عملته مخلة لزيتون و عفن طلع فوق هذا + +434 +00:32:13,780 --> 00:32:17,700 +الأبيض و المخلة الخيار هذا اسمه biofilm ليش + +435 +00:32:17,700 --> 00:32:22,000 +البكتيريا طاع فيه على سطر هذه بكتيريا لأن هي عندها + +436 +00:32:22,000 --> 00:32:25,020 +المقدرة على ال attachment بينها و بين بعضها لكن + +437 +00:32:25,020 --> 00:32:27,780 +هناك بكتيريا عندها المقدرة على ال attachment بينها + +438 +00:32:27,780 --> 00:32:32,640 +و بين الأسطح تخيلوا يا ابنات مكينة غذاء بنقطع فيها + +439 +00:32:32,640 --> 00:32:37,990 +غذاءبقايا بيضل شوية بقايا فبتجي البكتيريا بتنمو + +440 +00:32:37,990 --> 00:32:40,950 +لما تنمو بتروح اللي هي ملازجة في الماكينة نفسها + +441 +00:32:40,950 --> 00:32:45,230 +ايه طبعا بنغسلها بنرشها بالمي احنا في اماكن يصعب + +442 +00:32:45,230 --> 00:32:49,970 +عينا الوصول الفرشاية فبتكون هناك هذه البكتيريا إذا + +443 +00:32:49,970 --> 00:32:54,180 +.. إذا من النوع اللي بيكون biofilmحتى لو حطينا + +444 +00:32:54,180 --> 00:32:58,620 +عليها كلور خد بالكوا البكتيريا ال behavior تبعها + +445 +00:32:58,620 --> 00:33:03,980 +لما بتكون single cells خلايا منفصلة بيختلف تماما + +446 +00:33:03,980 --> 00:33:08,860 +عن ما لو كانت biofilm يعني لو كنا نقتلها بتركيز 3% + +447 +00:33:08,860 --> 00:33:14,700 +كلور تمام بدنا 6% كلور لل biofilm او 10% كلورين لل + +448 +00:33:14,700 --> 00:33:18,500 +biofilm ال behavior تبع ال biofilm بيختلف احد + +449 +00:33:18,500 --> 00:33:22,850 +مظاهر الاختلاف هو its ability to resistresist + +450 +00:33:22,850 --> 00:33:25,930 +what? different methods of disinfection and + +451 +00:33:25,930 --> 00:33:29,370 +sterilization طب الكلام اللي بحكيه أنا هذا ما هو + +452 +00:33:29,370 --> 00:33:32,330 +نفسه بينطبق على المستشفى لما أنا أركب canula + +453 +00:33:32,330 --> 00:33:36,370 +للمريض ال canula وإن كانت مصموعة من ال plastic + +454 +00:33:36,370 --> 00:33:40,210 +تمام و ال plastic ناعم و هي معقمة و تمام تمام إلا + +455 +00:33:40,210 --> 00:33:45,850 +أنه بالنسبة للبكتيريا هي جسم خشن جدا بالنسبة لحجم + +456 +00:33:45,850 --> 00:33:50,590 +البكتيريا خشن جدا و تستطيع أن تبقى ملتصقا فيه + +457 +00:33:51,680 --> 00:33:54,820 +تعرفوا انتوا مرضى الشلل الرابعى او النصف اللى + +458 +00:33:54,820 --> 00:33:59,540 +بيصير انه شلل بعد نتيجة حادث او كده طبعا بيصيروا + +459 +00:33:59,540 --> 00:34:03,100 +يفقدوا السيطرة على التبول فبيركبولهم قاسترة بولية + +460 +00:34:03,100 --> 00:34:06,960 +قاسترة بولية اللى بيدخل انبوب الى داخل المثارة عبر + +461 +00:34:06,960 --> 00:34:12,220 +اليوريثرة سواء male او female هلاجى انه الشخص هذا + +462 +00:34:12,220 --> 00:34:16,480 +اللى دخلناله قاسترة صار عنده urinary tract + +463 +00:34:16,480 --> 00:34:21,950 +infection باستمرارليش؟ لأنه القسطرة .. الجزء اللي + +464 +00:34:21,950 --> 00:34:27,650 +دخل جوا من القسطرة أصبح matrix، يعني مكان لل + +465 +00:34:27,650 --> 00:34:31,270 +biofilm للبكتيريا، بنعالجه، بنقتل البكتيريا اللي + +466 +00:34:31,270 --> 00:34:34,930 +كانت single cells لحالها، اللي عاملة infection، + +467 +00:34:34,930 --> 00:34:37,710 +لكن لا انا استطيع اقتل مين؟ ال biofilm اللي موجود + +468 +00:34:37,710 --> 00:34:40,770 +طب بنموت البكتيريا، بنطيب المريض، بعد يوم أو + +469 +00:34:40,770 --> 00:34:43,370 +يومين، بنتوقع من إيقاف ال antibiotic، بيرجع مرة + +470 +00:34:43,370 --> 00:34:48,550 +أخرى ال infectionخلصت الموضوع، لو جعدنا مئة سنة + +471 +00:34:48,550 --> 00:34:52,230 +نتكلم عن ال attachment، هنلاقي مواضيع نتكلم فيها، + +472 +00:34:52,230 --> 00:34:57,350 +من أهمية هذا الموضوع، إذن ال attachment سواء كان + +473 +00:34:57,350 --> 00:35:00,450 +على مستوى ال pathogenesis أو على مستوى ال + +474 +00:35:00,450 --> 00:35:03,490 +survival، و ال survival أنا فهمني في المستشفى، أنا + +475 +00:35:03,490 --> 00:35:06,070 +الدرس الماضي كان عن ال disinfection و ال + +476 +00:35:06,070 --> 00:35:08,930 +sterilization، مظبوط ولا لأ؟إذا ماكانش هندي + +477 +00:35:08,930 --> 00:35:12,670 +المقدرة بتعطيكوا مثال واقي من أبحاثنا اللي عملناها + +478 +00:35:12,670 --> 00:35:15,450 +كدراسة مجموعة من طالبات قسم الأعلام الطبية + +479 +00:35:15,450 --> 00:35:19,470 +المخبرية عملوا دراسة على المياه اللي بتشربوها في + +480 +00:35:19,470 --> 00:35:22,810 +الجامعة اللي بتلاقوها في الحنافيات أو ال boxes هذه + +481 +00:35:24,380 --> 00:35:28,480 +طبعا مصدرها واحد احنا خدنا من جهنم مصدر بلدية + +482 +00:35:28,480 --> 00:35:31,600 +وقعنا مصدر ال filter وقعنا مصدر بيئر .. بيئر خاص + +483 +00:35:31,600 --> 00:35:34,700 +بالجامعة واخدوا عينات من كل الأماكن المهم خلينا في + +484 +00:35:34,700 --> 00:35:37,780 +موضوعنا المياه اللي انتوا تشربوا منها اللي هي جام + +485 +00:35:37,780 --> 00:35:43,000 +الفلتر لجينا ان الفلتر بعطي ميه كويسة لكن لجينا + +486 +00:35:43,000 --> 00:35:46,740 +بعض ال .. الوحدات ال terminals تمام مابتعطيش ميه + +487 +00:35:46,740 --> 00:35:50,940 +كويسة فيها micro organismsفأطول بقتصرنا في شغل + +488 +00:35:50,940 --> 00:35:53,940 +إدارية وقلنا لهم يا جماعة المياه اللي عندنا في + +489 +00:35:53,940 --> 00:35:56,540 +الجامعة في بعض الأماكن واحد اتنين تلاتة أربع عملهم + +490 +00:35:56,540 --> 00:36:00,120 +زي خارطة انه يجب عليك تتوقفوا ال system يوم الخميس + +491 +00:36:00,120 --> 00:36:04,420 +و الجمعة يصير في الخميس او الأربعة و الخميس و + +492 +00:36:04,420 --> 00:36:08,200 +الجمعة و يصير this disinfection و الجماعة الصحيح + +493 +00:36:08,200 --> 00:36:10,720 +كانوا متعاونين جدا الإخوة في شغل إدارية و جاموا + +494 +00:36:10,720 --> 00:36:14,560 +بحملة رائعة جدا و نظفوا كل الأماكن مثل البنات يوم + +495 +00:36:14,560 --> 00:36:17,120 +السبت وروحوا خدوا عينات هتلاقوا البكتينة أكتر من + +496 +00:36:17,120 --> 00:36:17,320 +دقيقة + +497 +00:36:20,370 --> 00:36:23,250 +جمعوا عينات بالفعل لو البكتيريا عددها أكبر من ذي + +498 +00:36:23,250 --> 00:36:29,010 +قبل، إيش اللي صار؟ إنه الأماكن هذه اللي عملوا فيها + +499 +00:36:29,010 --> 00:36:33,370 +عملية تنظيف هم أثار ال biofilms، يعني عملوا تنظيف + +500 +00:36:33,370 --> 00:36:36,630 +فال biofilm اللي كانت راكدة وتعطي عدد معين من + +501 +00:36:36,630 --> 00:36:41,410 +البكتيريا باستمرار، مش استفزوها، أثروها بمعنى إن + +502 +00:36:41,410 --> 00:36:45,210 +هم بنفسهم أزقوهافتمظيقها الآن صار مورد الآن + +503 +00:36:45,210 --> 00:36:48,190 +بالسائق، فقالوا مضط في ال system كمان مرة وضخوا + +504 +00:36:48,190 --> 00:36:51,230 +مياه سخنة في ال system وضخوا مياه .. او chlorine + +505 +00:36:51,230 --> 00:36:53,590 +dosage جولة عالية و بعدين يغسلوا ال system و + +506 +00:36:53,590 --> 00:36:57,410 +بالفعل صار عملية التنظيف، القصد من الكلام أنه + +507 +00:36:57,410 --> 00:37:01,070 +طريقة الت .. الت .. التنظيف اختلفت لإن البكتيريا + +508 +00:37:01,070 --> 00:37:05,610 +كانت عاملة ايه؟ بيوم، اه دي نقطة كتير، يبقى ال + +509 +00:37:05,610 --> 00:37:06,550 +attachment بنات + +510 +00:37:09,760 --> 00:37:14,240 +ميزة للبكتيريا إذا انتلقتها .. انتلقت يعني صفات + +511 +00:37:14,240 --> 00:37:21,420 +إيجابية إلها تؤهلها لل survival بشكل جيد طيب + +512 +00:37:21,420 --> 00:37:24,980 +النقطة التانية اللي بعد ال attachment اللي هي ال + +513 +00:37:24,980 --> 00:37:29,360 +invasion of host cell antigen اتفقنا إنه في + +514 +00:37:29,360 --> 00:37:33,840 +إمكانية لل invasion أو localized infection خلنا + +515 +00:37:33,840 --> 00:37:36,660 +نجسم ال infection اسمه .. نسميها localized و + +516 +00:37:36,660 --> 00:37:40,460 +systemic إيش يعني localized؟يعني محددة في local + +517 +00:37:40,460 --> 00:37:44,300 +area معينة زي التيتانوس ما كنا نقول أصعب منطقة من + +518 +00:37:44,300 --> 00:37:47,900 +الجلب أو من العضل و بقية هناك بينما السايمونيلا + +519 +00:37:47,900 --> 00:37:52,000 +تايفي انتقلت من ال intestine إلى ال blood إلى ال + +520 +00:37:52,000 --> 00:37:55,760 +gallbladder إلى ال ال central nervous system و هذا + +521 +00:37:55,760 --> 00:38:00,420 +زمناها systemic ما الذي يؤهل البكتيريا إنها تعمل + +522 +00:38:00,420 --> 00:38:01,440 +invasion؟ + +523 +00:38:06,440 --> 00:38:13,660 +لكن لا بأس إننا نتطلع على المسميات وبعدين نناقش ال + +524 +00:38:13,660 --> 00:38:19,120 +function تبع كل مسمى منهم الاسم الأول + +525 +00:38:19,120 --> 00:38:24,420 +Hyaluronidase، الاسم التاني collagenase، الاسم + +526 +00:38:24,420 --> 00:38:29,180 +التالف neuroaminidase، الرابع coagulase، الخانس + +527 +00:38:29,180 --> 00:38:34,720 +kinase، لاحظوا الشغل؟كلهم aces يعني enzymes وكل + +528 +00:38:34,720 --> 00:38:39,000 +واحد اسمه معاه اسمه معاه coagulation بيعمل + +529 +00:38:39,000 --> 00:38:44,700 +coagulation ال kinases مابتعرفوهاش بتحلل نعم بتحلل + +530 +00:38:44,700 --> 00:38:50,460 +ال clots عكس ال coagulase بره لابن الجرح كيف الرسم + +531 +00:38:50,460 --> 00:38:53,700 +بيعمل healing؟ أحد بيعمل ال clotting بيعمل ال + +532 +00:38:53,700 --> 00:38:57,620 +clotting مظبوط ولا لأ؟ تخيلوا لو بكتيريا من النوع + +533 +00:38:57,620 --> 00:39:02,500 +اللي بنتش kinase قلت و استقرت في الجرحكل ما يتكوّن + +534 +00:39:02,500 --> 00:39:07,460 +الكلتش هتعملها .. هتعمله عملية تحليل للجلطة على + +535 +00:39:07,460 --> 00:39:11,000 +فكرة هذه استخدامات ظاهرة في علاج أمراض الجلطة طبعا + +536 +00:39:11,000 --> 00:39:14,060 +هبيروح على المستشفى على مرض القلب يلاقي عندهم علاج + +537 +00:39:14,060 --> 00:39:17,860 +غير ثمن جدا غير اسمه streptokinase streptokinase + +538 +00:39:17,860 --> 00:39:21,720 +ماخود من streptococcus من streptococcus حينك سموه + +539 +00:39:21,720 --> 00:39:25,480 +streptokinase في استفيدوا كينيز لكن لا يستخدم لا + +540 +00:39:25,480 --> 00:39:31,120 +يستخدمال coagulase .. ال coagulase بيحول ال + +541 +00:39:31,120 --> 00:39:38,100 +fibrinogen إلى fibrin عكس اللي هو ال kinase تخيلوا + +542 +00:39:38,100 --> 00:39:41,860 +ال Staphylococcus aureus تنتج coagulase و الموضوع + +543 +00:39:41,860 --> 00:39:45,440 +ده في دمنا و كل ما جاب ال fibrinogen بتحوله ل + +544 +00:39:45,440 --> 00:39:49,400 +fibrin يعني هتعمل clots في الجسم، هتعمل جلطات في + +545 +00:39:49,400 --> 00:39:53,180 +الجسمطبعا هي .. هي مش قصدها انها تقتل ال host في + +546 +00:39:53,180 --> 00:39:55,920 +الإيطار و انما قصدها تحمي نفسها .. احنا بدنا ندافع + +547 +00:39:55,920 --> 00:39:59,320 +عن البكتيريا .. احنا الكل بي .. الكل ضدها .. احنا + +548 +00:39:59,320 --> 00:40:02,060 +علىقل نكون يعني من الناس اللي بيدافع عن البكتيريا + +549 +00:40:02,060 --> 00:40:06,000 +.. هي تقصد انها تحمي نفسها من اللي هو ال immune + +550 +00:40:06,000 --> 00:40:09,940 +system تبع الإنسان انها تكون داخل شيء لزج .. داخل + +551 +00:40:09,940 --> 00:40:13,680 +شيء لزج اللي هو ال blood فهو بيحميها من ال immune + +552 +00:40:13,680 --> 00:40:17,520 +system .. لكن دون أن تقصد قد تقتلنا .. مافي مشكلة + +553 +00:40:17,520 --> 00:40:20,280 +.. يعني مبدأ مش دون قصد .. يعني ال D تبعت غير .. + +554 +00:40:20,550 --> 00:40:26,190 +الدية تبعتها رهيب طيب .. طب مين .. مين .. مين + +555 +00:40:26,190 --> 00:40:29,590 +بالكه أنجح؟ ال host .. اه عفوا .. ال .. ال .. ال + +556 +00:40:29,590 --> 00:40:34,910 +.. ال pathogen القاتل ولا ال pathogen اللي ممكن + +557 +00:40:34,910 --> 00:40:40,330 +يعيش معانا بدون ما يقتلنا؟ انه أنجح؟ من وجه نظر .. + +558 +00:40:40,330 --> 00:40:43,390 +من وجه نظر ال pathogen نفسه؟ انه أحسنله؟ + +559 +00:40:45,860 --> 00:40:51,000 +يعني بما ناخر بيسكتر و بيبقينا حيان لإنه هيطيل من + +560 +00:40:51,000 --> 00:40:56,400 +فترة حياته هو، بيطل فترة حياتنا احنا، أما لو قتلنا + +561 +00:40:56,400 --> 00:41:00,240 +تمام زي ال anthrax فهو .. احنا هنموت لإن هو مش + +562 +00:41:00,240 --> 00:41:03,960 +هلاقي غضب و كده ليه هيموت، إذا للأنجح إله إنه .. + +563 +00:41:04,250 --> 00:41:08,970 +يبقى زي .. زي ال .. البكتيروفاج لما دخل الخلية و + +564 +00:41:08,970 --> 00:41:13,310 +جالهم انا بس ماعليش يعني .. مؤقتا يعني .. اه و + +565 +00:41:13,310 --> 00:41:16,950 +انقسم من قسام الخلية، هذا كان أنجح من مين؟ من أخوه + +566 +00:41:16,950 --> 00:41:23,470 +اللي دخل و حلل الخلية بشكل عنيف فين حالة اسمها + +567 +00:41:23,470 --> 00:41:27,210 +streptomycin؟ اعفوا، ليوكوسايدين، ليوكوسايدين + +568 +00:41:27,210 --> 00:41:31,910 +ليوكوسايدين كلمة .. سايدين معناها .. من جاية من + +569 +00:41:31,910 --> 00:41:37,020 +كلمة سايدل، سايدل القتلاليوكو أبيض القاتل الأبيض + +570 +00:41:37,020 --> 00:41:40,940 +يعني مالأي قاتل كورات الدم البيضاء قاتل لكورات + +571 +00:41:40,940 --> 00:41:45,160 +الدم البيضاء هذا متخصص البكتيريا تنتجه عشان يقتل + +572 +00:41:45,160 --> 00:41:48,020 +كورات الدم البيضاء عشان نعرف ان كورات الدم البيضاء + +573 +00:41:48,020 --> 00:41:54,280 +المصبوط ال macro phage و ال phagocytes كلها من + +574 +00:41:54,280 --> 00:41:58,140 +اللي تنتمي إلى ال white blood cell فلما نلاقي خلية + +575 +00:41:58,140 --> 00:42:02,320 +قادرة على قتل ال white blood cell هذا مؤشر يعني + +576 +00:42:02,320 --> 00:42:06,360 +أنه البكتيرياعندها virulence factor J نيجي لل + +577 +00:42:06,360 --> 00:42:09,200 +Striptolysin والهيموليسين الصحيح أنه .. يعني + +578 +00:42:09,200 --> 00:42:13,220 +الهيموليسين أشمل لإن ال Striptolysin هو هيموليسين + +579 +00:42:13,220 --> 00:42:17,480 +بس تنتجه اللي هو ال Striptococcus يعني متخصص بال + +580 +00:42:17,480 --> 00:42:22,560 +Striptococcus هيموليسين بيعمل هيموليس لمين؟ لل .. + +581 +00:42:22,560 --> 00:42:26,080 +هو لل Red Blood Cell أكتر شي عشان يسموه Hemo .. + +582 +00:42:26,080 --> 00:42:30,870 +Hemo و ال Hem في ال Red Blood Cellطبعا + +583 +00:42:30,870 --> 00:42:33,670 +الستربتولاسين هو Hemolysin بانجام مرة بس بتنتج + +584 +00:42:33,670 --> 00:42:38,390 +لستربتو كوكس لسه تنايز و phospholipases برضه نفس + +585 +00:42:38,390 --> 00:42:43,530 +الفكرة ال .. ال .. ال .. ال phospholipases اسم عام + +586 +00:42:43,530 --> 00:42:46,910 +لمجموعة من الانزيمات قادرة على تحليل ال + +587 +00:42:46,910 --> 00:42:50,330 +phospholipidsأحد ال phospholips المشهورة اللي هو + +588 +00:42:50,330 --> 00:42:54,990 +ال licitin بتنتجه بكتيريا زي كلوسترديوم بنسميه + +589 +00:42:54,990 --> 00:43:00,150 +licitinase واسم تاني حتى phospholipase C كده ال + +590 +00:43:00,150 --> 00:43:04,330 +licitin أو عفوا licitinases هي تبع لمين؟ لل + +591 +00:43:04,330 --> 00:43:10,460 +phospholipaseفي عندنا anthrax enema factor و + +592 +00:43:10,460 --> 00:43:15,900 +pertosis adenylate cyclase enzyme AC اختصار ال + +593 +00:43:15,900 --> 00:43:20,020 +adenylate cyclase enzyme ناخد الأخير adenylate + +594 +00:43:20,020 --> 00:43:23,660 +cyclase enzyme بتعرفوا حاجة اسمها Cyclic A and B؟ + +595 +00:43:23,660 --> 00:43:29,500 +Cyclic A and B طيب بتعرفوا حاجة اسمها ATP؟ ال + +596 +00:43:29,500 --> 00:43:34,900 +Cyclic A and B أحد منتجات اللي هو ال ATPخد بالك ال + +597 +00:43:34,900 --> 00:43:39,700 +Cyclic AMP الوظائف رائعة جدا في الجسم ومحددة + +598 +00:43:39,700 --> 00:43:47,640 +وكميته على قدر وظيفته إذا زادت كميته زادت وظيفته + +599 +00:43:47,640 --> 00:43:52,620 +ومشكلة إنه تزيد الوظيفة أنا بدي أنتج عشر مركبات في + +600 +00:43:52,620 --> 00:43:56,920 +اليوم هاي طاقة الإنتاجية وبيجوبوا بالمهمة تماما + +601 +00:43:56,920 --> 00:43:58,300 +عشرة لو أنتجت مية + +602 +00:44:01,530 --> 00:44:04,590 +يبقى مانع أكثر في خلل في ال system اللي أنا مخططه + +603 +00:44:04,590 --> 00:44:09,490 +علشان أن أنت فقط عشان ال adenylate cyclase enzyme + +604 +00:44:09,490 --> 00:44:15,850 +بيعمل نشاط معين يؤدي هذا النشاط إلى إنتاج كمانية + +605 +00:44:15,850 --> 00:44:20,290 +كبيرة من من؟ من ال cyclic AMP فال cyclic AMP بدل + +606 +00:44:20,290 --> 00:44:22,950 +ما يجم بالوظيفة المحدودة للجدي صارت جم بالوظيفة + +607 +00:44:22,950 --> 00:44:28,000 +كبيرة فتؤدي إلى خلل كلمة خلل ما أنتوا عارفين؟عطيكم + +608 +00:44:28,000 --> 00:44:30,620 +مثال على أديناليت سايكليز أنزايم ال Vibrio cholera + +609 +00:44:30,620 --> 00:44:35,820 +بتنتج cholera تكسين لو وصفناه احنا بنوصفه على أنه + +610 +00:44:35,820 --> 00:44:41,640 +أديناليت سايكليز أنزايم بيرتبط التكسين بالأديناليت + +611 +00:44:41,640 --> 00:44:46,420 +سايكليز بيعمله activation لما يصير activation + +612 +00:44:46,420 --> 00:44:51,240 +للأديناليت سايكليز أنزايم بيتحول ال ATP لcyclic + +613 +00:44:51,240 --> 00:44:54,620 +AMP يبقى صرفين ينتج كمية كبيرة من مين؟ من ال + +614 +00:44:54,620 --> 00:44:59,260 +cyclic AMPالانستكليك EMB بيمنع ال absorption، + +615 +00:44:59,260 --> 00:45:02,340 +مظيفته يمنع ال absorption تبع ال chloride و يزيد + +616 +00:45:02,340 --> 00:45:05,800 +من ال secretion تبع ايه؟ الصوديوم، لما أصير في + +617 +00:45:05,800 --> 00:45:10,020 +عندي صوديوم في هذه الأمعاء، تخيلوا الأمعاء مصورة، + +618 +00:45:10,020 --> 00:45:13,640 +صار عندي عدم امتصاص لل chloride و إنتاج لمين؟ لل + +619 +00:45:13,640 --> 00:45:17,820 +sodium، فصار عندي كمية كبيرة من ال chloride جوا و + +620 +00:45:17,820 --> 00:45:22,400 +ال sodium، ال chloride و ال sodium و ال calcium، + +621 +00:45:22,400 --> 00:45:25,910 +هدول عبارة عن electrolytesإذا زادت كمية الـ + +622 +00:45:25,910 --> 00:45:29,070 +electrolyte في الـ human of the intestine الجسم + +623 +00:45:29,070 --> 00:45:34,030 +بيشعر إن هذا خطر يهدد حياة الإنسان فبروح بينتج + +624 +00:45:34,030 --> 00:45:37,630 +كمية كبيرة من إيه؟ من السائل اللي هو الميه to + +625 +00:45:37,630 --> 00:45:42,890 +dilute لإخفف دياريا is produced وصلت الصورة + +626 +00:45:42,890 --> 00:45:48,630 +فتخيلوا إن هذا الانيليزا غليز انزايم مش موجود له + +627 +00:45:48,630 --> 00:45:52,370 +تجويف في المكان اللي هو فيه زي الpertosis مثلا، + +628 +00:45:52,370 --> 00:45:57,130 +تمام؟الحقيقة ماتقلش الآن ما يسمى بالادمة اللي هي + +629 +00:45:57,130 --> 00:46:02,390 +الانتفاق في الاستثقاء اللي هو انه ميه تنزل في + +630 +00:46:02,390 --> 00:46:05,330 +الأنسجة extra cellular water لأنه برضه عملية + +631 +00:46:05,330 --> 00:46:09,130 +dilution لمين ده ال Electrolyte المرة القادمة ان + +632 +00:46:09,130 --> 00:46:14,210 +شاء الله بنأكد على بعض الزيادة تبع ال virus factor + +633 +00:46:14,210 --> 00:46:17,790 +هذا اللي هنتكلم فيه و بعض الناس هياخد معانا فترة + +634 +00:46:17,790 --> 00:46:22,640 +طويلةاللي بناخده في أول المحاضرة هيزمها في + +635 +00:46:22,640 --> 00:46:24,440 +المحاضرة التانية أو بالتالتة أو بالرابعة أو + +636 +00:46:24,440 --> 00:46:27,340 +بالخمسة فانا أرجو من حضراتكم ان انتوا تقراوا معانا + +637 +00:46:27,340 --> 00:46:27,740 +باسم الله + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/f2aiJbJwTRQ_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/f2aiJbJwTRQ_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..1519102a8c4f290a1dbbf5097f68476f1cd8df57 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/f2aiJbJwTRQ_raw.srt @@ -0,0 +1,2548 @@ +1 +00:00:20,690 --> 00:00:24,770 +بسم الله الرحمن الرحيم الموضوع ما زلنا في ال + +2 +00:00:24,770 --> 00:00:29,290 +bacterial infection and immunity وحنعمل quick + +3 +00:00:29,290 --> 00:00:33,410 +revision للما بدأنا بيه المرة الماضية تحدثنا عن + +4 +00:00:33,410 --> 00:00:37,590 +ثلاث مصطلحات متعلقة بوصف العلاقة بين كائنين اللي + +5 +00:00:37,590 --> 00:00:40,550 +هم ال commercialism و الparasitism بالإضافة إلى ال + +6 +00:00:40,550 --> 00:00:44,030 +mutualism و أكدنا على أنه الparasitism هي العلاقة + +7 +00:00:44,030 --> 00:00:44,730 +الأكتر شيء + +8 +00:00:47,840 --> 00:00:51,260 +تحدثنا عن ال normal microbiota و بعض الفوائد + +9 +00:00:51,260 --> 00:00:56,240 +المتنوعة اللي شملناهم تقريبا هم أربع نقاط لكن كنا + +10 +00:00:56,240 --> 00:00:59,780 +عنهم تلاتة ممكن منهم تلاتة أفضل الأولى vitamin K + +11 +00:00:59,780 --> 00:01:03,300 +رقم اتنين ال colonization resistance و تمثل في + +12 +00:01:03,300 --> 00:01:07,420 +شغلتين اللي هو occupying the space and producing + +13 +00:01:07,420 --> 00:01:11,620 +chemicals زي ال bacteriocins و تحدثنا عن ان ال + +14 +00:01:11,620 --> 00:01:14,220 +normal flora قد تساعد في ال development لبعض ال + +15 +00:01:14,220 --> 00:01:17,980 +organs زي ال viral patches اللي عبارة عن مجموعةمن + +16 +00:01:17,980 --> 00:01:22,360 +ال lymph nodes موردة في ال intestinal tract كمان + +17 +00:01:22,360 --> 00:01:25,820 +تطرقنا إلى الفرق ما بين ما يسمى بال hospital + +18 +00:01:25,820 --> 00:01:28,640 +infection وما بين اللي هو ال community acquired + +19 +00:01:28,640 --> 00:01:33,740 +infection وحاولنا ان اكد على مفهوم ان الميكروبات + +20 +00:01:33,740 --> 00:01:38,360 +المكتبة في ال health care institutions are usually + +21 +00:01:38,360 --> 00:01:41,960 +more resistant to antibiotic than those acquired + +22 +00:01:41,960 --> 00:01:46,130 +in the community هذه كانت المفهوم العاموإنه هناك + +23 +00:01:46,130 --> 00:01:49,270 +قد يحدث تغيير في ال normal microbiota ما بين + +24 +00:01:49,270 --> 00:01:53,150 +الموجود في المجتمع وبين الموجود في هذه المؤسسة + +25 +00:01:53,150 --> 00:01:58,570 +وهذه اللوحة كانت تلخيص لهذا الكلام ويبدأ بالحديث + +26 +00:01:58,570 --> 00:02:01,230 +عن مصطلحات مثل pathogen و non pathogen و + +27 +00:02:01,230 --> 00:02:05,650 +opportunistic pathogen و pathogenisticالأن ال + +28 +00:02:05,650 --> 00:02:08,510 +pathogen هو من يمتلك ال pathogenicity وال non + +29 +00:02:08,510 --> 00:02:11,790 +pathogenic هو من لا يمتلك ال pathogenicity ال + +30 +00:02:11,790 --> 00:02:15,070 +opportunistic pathogen هو يمتلك ال pathogenicity + +31 +00:02:15,070 --> 00:02:18,470 +لما يكون الشخص اللي أمامه immunocompromised أما + +32 +00:02:18,470 --> 00:02:23,210 +لما يكون immunocompetent فلا يستطيع أنه يحدث هذا + +33 +00:02:23,210 --> 00:02:29,050 +المرضواتطلقنا الي مصطلح اسمه virulence يقول ان هو + +34 +00:02:29,050 --> 00:02:33,070 +عبارة عن quantitative measure مقياس كم مي لمدى + +35 +00:02:33,070 --> 00:02:37,530 +البعاثة جنسدي و جسدنا اما بال LD50 او ال ID50 + +36 +00:02:37,530 --> 00:02:43,150 +وشرحنا ان ال ID50 هي الجرعة اللازمة لاحداث عدوى + +37 +00:02:43,150 --> 00:02:47,810 +عند 50% من حيوانات التجارب او ال test animal LD50 + +38 +00:02:47,810 --> 00:02:53,950 +بدل ما هي infection بتسبب وفيات او قتلأعتقد أنها + +39 +00:02:53,950 --> 00:03:00,410 +واجهة في نفسي عند اللوحة هذه أحد أهم العناصر اللي + +40 +00:03:00,410 --> 00:03:03,270 +بتساهم في ال virulence حاجة اسمها adherence + +41 +00:03:03,270 --> 00:03:06,550 +ولاحظوا ال adherence أنا معلم على ثلاث كلمات في + +42 +00:03:06,550 --> 00:03:10,050 +اللوحة adherence, adhesion أو attachment كلهم + +43 +00:03:10,050 --> 00:03:14,990 +بيبقى نفس الإحاء المهم ان نفهم ان ال adhesion + +44 +00:03:14,990 --> 00:03:21,130 +غالبا specific ايش ان specific يعني مركب ما مركب + +45 +00:03:32,630 --> 00:03:37,070 +الانفجار هو مفهومه + +46 +00:03:37,070 --> 00:03:41,450 +إن الميكروب قادر على انتقال من مكان الإصابة إلى + +47 +00:03:41,450 --> 00:03:48,030 +أماكن أخرى في غزو في غزو للأنسجة فمن يمتلك انفجار + +48 +00:03:48,030 --> 00:03:51,310 +انفجار يمتلك المقدر على انه ينتقل من مكان إلى مكان + +49 +00:03:51,310 --> 00:03:56,740 +أخرالسلمونيلا طايفي نشربها أو بناكلها مع الغذاء + +50 +00:03:56,740 --> 00:04:02,940 +السلمونيلا طايفي بسبب تايفويد فيبر السلمونيلا + +51 +00:04:02,940 --> 00:04:06,580 +طايفي بتتكاثر في الأمعاء لكن لا تكتفي من مكوثها في + +52 +00:04:06,580 --> 00:04:09,440 +الأمعاء تختارق في دار الأمعاء بتروح على ال lymph + +53 +00:04:09,440 --> 00:04:12,360 +node و من ال lymph node بتروح على ال blood و بتصير + +54 +00:04:12,360 --> 00:04:16,660 +موجودة في الدم و من الدم قد تنتقل إلى ما يسمى + +55 +00:04:16,660 --> 00:04:19,360 +pericardial endothelial system من ضمنها ال liver و + +56 +00:04:19,360 --> 00:04:24,160 +الفي ال bone مرة ممكن و ممكن تصل لل central + +57 +00:04:24,160 --> 00:04:28,760 +nervous system بتعمل هنا meningitis غزوا ولا مش + +58 +00:04:28,760 --> 00:04:34,580 +غزوا؟ غزوا بينما ال eclostridium tetany ال بتاع + +59 +00:04:34,580 --> 00:04:43,590 +مغيتروس بتنغز في مكان محدد و تبقى هناكولا تستطيع + +60 +00:04:43,590 --> 00:04:46,590 +إنها تنتقل من مكان الإصابة إلى مكان أخر، بينما + +61 +00:04:46,590 --> 00:04:52,010 +الـtoxin تبعها هو اللي بينتقل، لكن هي localized، + +62 +00:04:52,010 --> 00:04:57,070 +الأولى systemic، انتشرت الأولى، انتشرت التانية، + +63 +00:04:57,070 --> 00:05:01,770 +localized، وبالتالي الأولى invasive والتانية non + +64 +00:05:01,770 --> 00:05:06,380 +-invasive، non-invasive، كتير مهم المفهوم هذاو ال + +65 +00:05:06,380 --> 00:05:10,920 +adherence و ال invasion يعني معناين مختلفين لكن + +66 +00:05:10,920 --> 00:05:14,500 +كلهم بيصبوا في ال virulence و ال virulence مقياس + +67 +00:05:14,500 --> 00:05:20,000 +للبعض هو innocent المصطلح التالت toxicity و برضه + +68 +00:05:20,000 --> 00:05:24,940 +هذا مصطلح يصب في نفس الإطار بكتيريا تمتلك تكسين و + +69 +00:05:24,940 --> 00:05:30,620 +بكتيريا لا تمتلك تكسين خدوا أبلغوا من شغلهناك بعض + +70 +00:05:30,620 --> 00:05:37,140 +الأمراض الأعراض كلها الناتجة عن المرض بتكون ناتجة + +71 +00:05:37,140 --> 00:05:40,700 +عن التوكسين نفسه زي التيتانوس اللى ذكرنا جاب شوية + +72 +00:05:40,700 --> 00:05:46,760 +البكتيريا اللى موجودة في القدم على سبيل المثال لكن + +73 +00:05:46,760 --> 00:05:50,660 +الأعراض متمثلة بشلل وشلل الاسباستيك بيرانسس + +74 +00:05:53,650 --> 00:05:58,210 +سببه مش الوجود البكتيريا وانما في التوكسين بمعنى + +75 +00:05:58,210 --> 00:06:01,690 +لو أخدنا البكتيريا و ربناها برا و أخدنا ال toxin + +76 +00:06:01,690 --> 00:06:05,390 +تبعها و حقنها في الإنسان فيحدث نفس الأعلى تبع ال + +77 +00:06:05,390 --> 00:06:09,370 +tetanus إذا لما تكون البكتيريا toxigenic تخلف على + +78 +00:06:09,370 --> 00:06:12,790 +ما يكون non-toxigenic بس بودأ أذكركوا يا بنات + +79 +00:06:12,790 --> 00:06:16,430 +بالمفهوم اللي مرض على صناعة لما اتكلمنا عن ال + +80 +00:06:16,430 --> 00:06:21,240 +bacterophageلما اتكلمنا عن ال isogenic cycle لما + +81 +00:06:21,240 --> 00:06:24,960 +اتكلمنا عن ال profage لداخل و لما اتكلمنا عن ال + +82 +00:06:24,960 --> 00:06:29,140 +isogenized cell و ان ان كتير من البكتيريا + +83 +00:06:29,140 --> 00:06:33,860 +التوكسوجينيك اصبحت توكسوجينيك لإن فيها profage، + +84 +00:06:33,860 --> 00:06:37,300 +هذه هي اللي تبقى لنا ذاكرينها السيد روبرت كوخ + +85 +00:06:37,300 --> 00:06:43,360 +للألمان و للكثيرين يعتبر هو أبو ال micro biology + +86 +00:06:43,360 --> 00:06:47,380 +الحديثة طبعاطبعا الفرنسيين بيقولوا لأ Louis + +87 +00:06:47,380 --> 00:06:52,760 +Pasteur لأن هو فرنسي فالتاريخ يكتبه من .. من يكتبه + +88 +00:06:52,760 --> 00:06:57,880 +Robert Koch من إنجازاته الكبيرة الصحيح أنه حاول + +89 +00:06:57,880 --> 00:07:03,380 +يعمل علاقة ما بين ال pathogen و ما بين المرض قال + +90 +00:07:03,380 --> 00:07:07,420 +إذا أردنا أن نثبت أن هذا الميكروب هو المسبب لهذا + +91 +00:07:07,420 --> 00:07:12,470 +المرض فعلينا الإجراءات التاليةالان خدوا بالكم ان + +92 +00:07:12,470 --> 00:07:17,250 +الفرضية كانت صالحة في ذاك الزمن و قاتت أكلها بمعنى + +93 +00:07:17,250 --> 00:07:23,750 +أثبت من خلالها هو و غيره كثير من المسببات للأمراض + +94 +00:07:23,750 --> 00:07:26,890 +فال Vibrio cholera هي المسببة للكوليرا ال + +95 +00:07:26,890 --> 00:07:29,330 +Mycobacterium tuberculosis هي المسبب للكوليرا + +96 +00:07:29,330 --> 00:07:33,830 +الbacillus anthracis هي المسببة للأنترا خلينا + +97 +00:07:33,830 --> 00:07:36,810 +نتكلم مع بعض و نطلع على ال animation + +98 +00:07:39,760 --> 00:07:43,680 +الفرضية الأولى كانت بتقول أنه الميكروب المسبب يجب + +99 +00:07:43,680 --> 00:07:48,140 +أن يكون موجود في الحيوان المصاب و ليس موجودا فيه + +100 +00:07:48,140 --> 00:07:53,180 +الحيوان السليم هذه أول خطوة في الفرضية الخطوة + +101 +00:07:53,180 --> 00:07:57,420 +التانية إذا لازم نعزل الميكروب من الحيوان المصاب + +102 +00:07:57,420 --> 00:08:04,380 +بصورة نقية عشان نعجب صورة نقية نحقنه في الحيوان + +103 +00:08:04,380 --> 00:08:09,230 +السليم يجب أن يظهر عليه نفس الأعراضواجب أن نقوم + +104 +00:08:09,230 --> 00:08:15,810 +بعزل الميكروب مرة أخرى، وصلت الصورة ولا لسه؟ إذا + +105 +00:08:15,810 --> 00:08:19,050 +ماوصلتش، نطلع على الخطوات، ياريت نركز على اللوحة + +106 +00:08:19,050 --> 00:08:24,910 +الآن، الـmicrobe is present in each case of the + +107 +00:08:24,910 --> 00:08:28,810 +disease, but not in healthy animals، أعتقد أن + +108 +00:08:28,810 --> 00:08:32,530 +الصورة الآن اللي يبقى بنا بتوضح أن الفقر المصاب + +109 +00:08:32,530 --> 00:08:35,870 +فيه الميكروب، بينما ال healthy animal ماهندوش + +110 +00:08:35,870 --> 00:08:41,460 +الميكروبالخطوة التانية إن نقوم بعزل ال microbe من + +111 +00:08:41,460 --> 00:08:45,320 +الفقر أو الحيوان اللي هو المصاب in pure culture + +112 +00:08:45,320 --> 00:08:48,420 +إذا + +113 +00:08:48,420 --> 00:08:51,640 +حققنا ال microbe اللي عزلناه من الأول في التاني + +114 +00:08:51,640 --> 00:08:58,440 +السليم يجب أن يحدث عنه نفس اللي هو العربي ويجب أن + +115 +00:08:58,440 --> 00:09:02,800 +نقوم بعزل ال microbe مرة أخرى من ال experimentally + +116 +00:09:02,800 --> 00:09:07,190 +infected اللي هو animalطب أنا قاعد بقول إن الفرضي + +117 +00:09:07,190 --> 00:09:10,730 +في ذلك الزمن قعدت أكلها و اليوم في عليها علامة + +118 +00:09:10,730 --> 00:09:14,870 +استفهام إيش علامة الاستفهام؟ الفرضي الأولى جلد إنه + +119 +00:09:14,870 --> 00:09:18,850 +يجب أن يكون في الحيوان المصاب ولا يزال في السلين + +120 +00:09:18,850 --> 00:09:23,190 +طب أنا بتعطيكم أمثلة كثيرة على مايكروبات موجودة في + +121 +00:09:23,190 --> 00:09:27,510 +المصاب وموجودة في السلين Staphylococcus aureus على + +122 +00:09:27,510 --> 00:09:30,710 +جلدنا موجودة وفي الفم تبعنا وعلى عينينا وعلى .. + +123 +00:09:30,710 --> 00:09:35,040 +يعني أماكن متنوعة من أجسامناو في نفس الوجهة في ناس + +124 +00:09:35,040 --> 00:09:38,900 +مرضى عندهم دمامل و خراجات و حبوب اللى بيطلع منها + +125 +00:09:38,900 --> 00:09:44,480 +الصدر الأبيض Staphylococcus aureus إذا ليس بالضلال + +126 +00:09:44,480 --> 00:09:47,980 +أن يكون ال microbe في ال .. في ال .. يعني ممكن + +127 +00:09:47,980 --> 00:09:50,660 +يكون مورود في التانية في ال .. لإن ال infection + +128 +00:09:50,660 --> 00:09:54,000 +كانت interaction من ثلاث عوامل فاكرينهم؟ ثلاث + +129 +00:09:54,000 --> 00:09:58,440 +عوامل ذكرناهم ال host immunity ال type يبقى في + +130 +00:09:58,440 --> 00:10:01,700 +عامل مهم جدا كان اللى هو ال host immunity مااخدوش + +131 +00:10:01,700 --> 00:10:06,310 +بقى يعني اللى هو الاعتباربدي اعطي مثال تاني بس + +132 +00:10:06,310 --> 00:10:10,090 +للاطمئنان في ميكروب مشهور جدا اسمه نيسيريا + +133 +00:10:10,090 --> 00:10:16,430 +مينينجيتايدس بتسبب التهاب السحية المينينجايتس هذا + +134 +00:10:16,430 --> 00:10:20,530 +موجود ك normal flora او ك normal microbiota في + +135 +00:10:20,530 --> 00:10:25,170 +أنوف اكثر من 15% من الناس اللي صحها يعني لو من 100 + +136 +00:10:25,170 --> 00:10:30,530 +طالبة أخدنا مسحات من الأنف ممكن نازل من 15% منهم + +137 +00:10:30,530 --> 00:10:37,800 +لمهام نيسيريا مينينجيتايدسهذا يضحط اللي هو ال band + +138 +00:10:37,800 --> 00:10:41,620 +الأول في فرضية كوخه إيش كمان في نقاط ضعف في فرضية + +139 +00:10:41,620 --> 00:10:47,320 +كوخه النقطة الثانية هي أنه أصر على أنه لازم نعزل + +140 +00:10:47,320 --> 00:10:52,060 +الميكروب بصورة نقية من الحيوان المصاب أو من حتى ال + +141 +00:10:52,060 --> 00:10:55,700 +experimental التاني طب هناك ميكروبات لحد الآن + +142 +00:10:55,700 --> 00:10:59,820 +ماقدرنا نميها في المختبر زي ال mycobacterium libri + +143 +00:10:59,820 --> 00:11:03,390 +اللي هي بسبب الجذع لحد الآن مالميناهاكتير من + +144 +00:11:03,390 --> 00:11:07,630 +الفيروسات لحد الآن ماقدرنا نميها في المختبر ومع + +145 +00:11:07,630 --> 00:11:11,790 +ذلك أثبتنا أنهي المسؤولة بطلوب أخرى غير اللي هو + +146 +00:11:11,790 --> 00:11:16,110 +فرضيتكوخ إذا فرضيتكوخ في ذاك الزمن كانت صالحة الآن + +147 +00:11:16,110 --> 00:11:18,750 +فيها عيبين أساسيا اللي هم العيب الأول اللي بكرناه + +148 +00:11:18,750 --> 00:11:21,710 +والتاني اللي هو متعلق بعزل ال micro + +149 +00:11:25,900 --> 00:11:31,700 +هذا ليس نقصا او انقاصا من حقوق Robert Koch بالعكس + +150 +00:11:31,700 --> 00:11:34,640 +يعني هو كان زي ما قلتلكوا انه من العلماء اللي .. + +151 +00:11:34,640 --> 00:11:38,200 +يعني اللي .. اللي سهموا وسامد عظيمة في وضع الصورة + +152 +00:11:38,200 --> 00:11:41,920 +نفس الفكرة اللي أمامكوا نفس الصورة اللي احنا + +153 +00:11:41,920 --> 00:11:46,610 +عرضناها في ال animationاللوحة هذه التالت اللي + +154 +00:11:46,610 --> 00:11:49,350 +موجودة فيها تلت دوائر اللي هم ال interaction ما + +155 +00:11:49,350 --> 00:11:54,010 +بين ال immune system تبع ال host و الجرعة و اللي + +156 +00:11:54,010 --> 00:11:57,610 +هو ال strain اللي جونا ال type و أنا ذكرت المحاضرة + +157 +00:11:57,610 --> 00:12:03,470 +السابقة قولت شجرة نحتاج الى واحد لمية microbe + +158 +00:12:03,470 --> 00:12:06,510 +تقريبا عشان نعمل infection عند الإنسان بينما ال + +159 +00:12:06,510 --> 00:12:10,670 +virus اللي جونا نحتاج الى مليون من واحد لمية يعني + +160 +00:12:10,670 --> 00:12:14,800 +ال number قليلو مليون number عالى يعني لو بدنا + +161 +00:12:14,800 --> 00:12:17,880 +نحطهم في الميزان تبع ال virulence بيقول مين is + +162 +00:12:17,880 --> 00:12:22,060 +more virulent الشجلة اللي هم عددها طبعا أقل اللي + +163 +00:12:22,060 --> 00:12:27,020 +هي عددها أقل كمان مرة ما ننساش التلات عوامل اللي + +164 +00:12:27,020 --> 00:12:30,020 +موجودين على اللوحة أمامنا اللي هو ال immune system + +165 +00:12:30,020 --> 00:12:33,620 +ال strength زي اللي هو ال virulence و أنا أضفت لهم + +166 +00:12:33,620 --> 00:12:38,520 +بُعد رابع المرة الماضية مين بيذكر البُعد الرابع ال + +167 +00:12:38,520 --> 00:12:43,370 +portal of entryمكان الدخول و ذكرتلكوا في المحاضرة + +168 +00:12:43,370 --> 00:12:47,430 +انه مثال ال .. ال .. الbacillus anthracis لو دخل + +169 +00:12:47,430 --> 00:12:51,110 +عبر الجلد او دخل عبر ال lungs او دخل عبر ال + +170 +00:12:51,110 --> 00:12:54,630 +gastrointestinal الأرقام اختلفت تماما اذا ال + +171 +00:12:54,630 --> 00:12:58,550 +portal of entry كمان كان بارد رابعا الصورة اللي + +172 +00:12:58,550 --> 00:13:03,810 +أمامكم بتتكلم عنbacterial virus factors انا لما + +173 +00:13:03,810 --> 00:13:07,710 +دراستكوا ال bacterial cell structure قولتلكوا + +174 +00:13:07,710 --> 00:13:13,470 +بناتنا الكلمات انه لسنا بصدق انه نحفظ بس التراكيب + +175 +00:13:13,470 --> 00:13:16,310 +انه التراكيب هدس ال wall هدس ال membrane و اين + +176 +00:13:16,310 --> 00:13:21,470 +ماكان اقولت لأ احنا بدنا نفهم ما ورا التراكيب و + +177 +00:13:21,470 --> 00:13:26,030 +كنا كتير عن التراكيب اعطيكم مثال بسيط جدا وسهل كنا + +178 +00:13:26,030 --> 00:13:30,510 +نقول عن ال flagella سمينها virus factor لانه بتهرب + +179 +00:13:30,510 --> 00:13:34,430 +من خطروبتذهب إلى غذاء، بتذهب إلى غذاء من خلال + +180 +00:13:34,430 --> 00:13:38,710 +الحركة أو تهرب من خطر في حال ان اتعرض هذا الخطر، + +181 +00:13:38,710 --> 00:13:42,570 +هذا اعتبرنا احنا by an instinct لما اتكلمنا على ال + +182 +00:13:42,570 --> 00:13:46,190 +attachment تحدثنا ايضا على ان ال attachment في + +183 +00:13:46,190 --> 00:13:50,770 +اناكن محددة ضرورة من ضرورات البقاء و ال + +184 +00:13:50,770 --> 00:13:53,830 +colonization تمام و بالتالي infection و بالتالي + +185 +00:13:53,830 --> 00:13:58,060 +diseaseيبقى كل التراكيب اللي درسناهم ما درسناهم + +186 +00:13:58,060 --> 00:14:04,320 +هكذا هكذا ان الان موعدهم ان احنا نعطيهم حقهم في ال + +187 +00:14:04,320 --> 00:14:09,780 +ال ال ال virans طيب + +188 +00:14:09,780 --> 00:14:13,980 +ماعرفش الجملة ماعرفش ليش مش واضحة في نقاط مش هتنزل + +189 +00:14:13,980 --> 00:14:18,660 +على اللوحة لكن بنقولكوا عنها regulation of + +190 +00:14:18,660 --> 00:14:23,350 +bacterial virance factorsبتير بيخطر على بال ان + +191 +00:14:23,350 --> 00:14:29,650 +احنا كبشر ان احنا كنا عند نفس المريض و تقريبا كنا + +192 +00:14:29,650 --> 00:14:33,330 +بنفس الدرجة من القرب من هذا المريض انا أصيبت + +193 +00:14:33,330 --> 00:14:37,430 +بالمرض و زميل لم يصاب لماذا انا اصيبت و هو لم يصاب + +194 +00:14:37,430 --> 00:14:42,330 +او العكس فتفسيراتنا بتقعد .. بنقعد .. بنحاول .. + +195 +00:14:42,330 --> 00:14:46,210 +نجتهد ان انت كنت اقرب اكتر او هو كان اقرب .. طب + +196 +00:14:46,210 --> 00:14:50,410 +هناك عوامل كثيرة احنا درست 3 عوامل قبل شوية منهم و + +197 +00:14:50,410 --> 00:14:55,060 +عملنا بواد رابع كنا منهماللي هو ال portal of + +198 +00:14:55,060 --> 00:15:00,560 +ending طيب ال strain .. ال strain عشان يعمل ال + +199 +00:15:00,560 --> 00:15:06,620 +virulenceفي حاجة بيسموها إما triggering، + +200 +00:15:06,620 --> 00:15:11,280 +triggering يعني إطلاق أو inciting حث، inciting + +201 +00:15:11,280 --> 00:15:14,440 +factors، triggering factors أو trigger اللي هو ضغط + +202 +00:15:14,440 --> 00:15:18,240 +الزناد يعني، تضغط على الزناد ليش؟ تضغط على الزناد + +203 +00:15:18,240 --> 00:15:22,060 +to produce the virulence factor و أحد أهمها + +204 +00:15:22,060 --> 00:15:24,520 +شرحتلكم المرة الماضية أنا حاسمها quorum sensing + +205 +00:15:24,520 --> 00:15:28,020 +فاكرينها؟ quorum sensing لما قلنا أنه عدد + +206 +00:15:28,020 --> 00:15:33,080 +البكتيريا، البكتيريا عندها المقدرة تشهرهو إذا + +207 +00:15:33,080 --> 00:15:36,760 +استشعت إن عددها صار مؤهل أن تنتج تكسين تنتج + +208 +00:15:36,760 --> 00:15:40,800 +التكسين هو ال virulence مش هو virus factor تكسين + +209 +00:15:40,800 --> 00:15:44,280 +طب إيش العوامل الأخرى اللي ممكن تأثر على إنتاج ال + +210 +00:15:44,280 --> 00:15:47,340 +virulence factors؟ أنا بقولوا أحدها ال + +211 +00:15:47,340 --> 00:15:54,640 +temperature، ال pH، ممكن ال osmolarity، ممكن ال + +212 +00:15:54,640 --> 00:16:01,060 +iron، ال iron يعني الحديدبعض ال ions المحددة إذا + +213 +00:16:01,060 --> 00:16:05,620 +ولد أو لم توجد كلها ممكن تساهم في switch on و + +214 +00:16:05,620 --> 00:16:10,780 +switch off يبقى + +215 +00:16:10,780 --> 00:16:16,140 +الآن لما بنيجي نفسر لماذا فلان أصيب و فلان لم يصاب + +216 +00:16:16,140 --> 00:16:19,800 +الأعمالية ازدادت تعقيدا العمالية ازدادت تعقيدا كيف + +217 +00:16:19,800 --> 00:16:23,980 +ازدادت تعقيدا احنا كنا متكلمين على تلات عوامل host + +218 +00:16:23,980 --> 00:16:30,500 +immunityو strain وش كمان؟ و الدوس طبعا طيب احنا + +219 +00:16:30,500 --> 00:16:34,200 +اتعرضنا لنفس ال strain و اتعرضنا لنفس الدوس على + +220 +00:16:34,200 --> 00:16:36,440 +سبيل المثال مين ضال الآن التالت؟ + +221 +00:16:40,870 --> 00:16:44,970 +طيب احنا ال immunity تبعتنا كانت determined عامل + +222 +00:16:44,970 --> 00:16:48,830 +محدد عامل محدد طب في كمان عامل محدد احنا اتنين + +223 +00:16:48,830 --> 00:16:52,930 +افترقنا عن بعض احنا اتنين افترقنا عن بعض انا تعرضت + +224 +00:16:52,930 --> 00:16:57,950 +لظروف و انت تعرضت لظروف اخرى قد تساهم في switching + +225 +00:16:57,950 --> 00:17:01,470 +on او switching off لمين لبعض ال virulence factors + +226 +00:17:01,470 --> 00:17:05,930 +اذا خلينا نقول من خلال معرفتنا بان في regulation + +227 +00:17:07,100 --> 00:17:10,120 +الانتاج تبقى ال violence factor مش دائما هو نفس + +228 +00:17:10,120 --> 00:17:15,340 +الانتاج بيعتمد على ظروف معينة بيئية وغذائية يبقى + +229 +00:17:15,340 --> 00:17:18,500 +معنا الحكي بنقدر نفسر لماذا هناك اختلافات في نسبة + +230 +00:17:18,500 --> 00:17:22,320 +الإصابة بين مجموعة من البشر اتعرضوا لنفس اللي ايه؟ + +231 +00:17:22,320 --> 00:17:26,280 +نفس الأوان اول + +232 +00:17:26,280 --> 00:17:31,940 +اولنقطة بنركز عليها اليوم في ال virulence factor + +233 +00:17:31,940 --> 00:17:34,860 +اللي هو ال adherence factor اللي يقول عنهم + +234 +00:17:34,860 --> 00:17:40,220 +adherence, adhesion او attachment اتولد صغير كتير + +235 +00:17:40,220 --> 00:17:44,600 +خالص اه يعني غير مرئي حتى بالنسبالي انا هتطلع ألبس + +236 +00:17:44,600 --> 00:17:52,250 +المضارة بدأ اقرأ بس بالأول أسماءللمراكبات اللي + +237 +00:17:52,250 --> 00:17:56,410 +كلها اعتبرناها adherence factors adhesin, + +238 +00:17:57,010 --> 00:18:02,710 +receptor, lectin, ligand, mucus, fimbria, common + +239 +00:18:02,710 --> 00:18:08,050 +pili, six pilas، اللي هي six pili يعني، pili جمع، + +240 +00:18:08,050 --> 00:18:12,710 +pilas اللي هي singular، type 1 fimbria، type 4 + +241 +00:18:12,710 --> 00:18:18,950 +pili, S layerالـ S Layer مرة عليكم Slime Layer و S + +242 +00:18:18,950 --> 00:18:22,010 +Layer و Capsule و Micro Capsule الجليكوكاليكس + +243 +00:18:22,010 --> 00:18:26,210 +الاسم العام لكلهم Capsule Lipopolysaccharide + +244 +00:18:26,210 --> 00:18:33,410 +Tychoic Acid and Lipotychoic Acid كل ما بكرناه هي + +245 +00:18:33,410 --> 00:18:37,510 +تراكيب موجودة في بعض أنواع من البكتيريا و غير + +246 +00:18:37,510 --> 00:18:43,410 +موجودة في بعض أنواع أخرى مع ده رقم اتنين اليمين ال + +247 +00:18:43,410 --> 00:18:49,570 +receptor ال receptorبنطلقه على التركيب اللي موجود + +248 +00:18:49,570 --> 00:18:52,270 +في خلايا ال E-Eukaryotes اللي هم ال host اللي هم + +249 +00:18:52,270 --> 00:18:56,030 +أحلى تمام اللي ممكن يرتبط فيها واحد من هذه + +250 +00:18:56,030 --> 00:19:01,390 +المركبات اللي هو البكتيروس الصورة بمعنى أخر أنه + +251 +00:19:01,390 --> 00:19:06,130 +مثلا الأدهسين بدهو receptor الليكتين بدهو receptor + +252 +00:19:06,130 --> 00:19:09,170 +الليجان بدهو receptor يبقى ال receptor هو المركب + +253 +00:19:09,170 --> 00:19:14,790 +المستقبل طيب في نقطة كتير مهمة أذكركوا و أرجع بيكم + +254 +00:19:14,790 --> 00:19:20,250 +بالبكتيروفاجالبكتيروفاج عمل infection لمن؟ + +255 +00:19:20,250 --> 00:19:25,090 +للبكتيريا لم يستطيع ان يعمل infection لا للنباتات + +256 +00:19:25,090 --> 00:19:31,730 +ولا للحيوانات تمام؟ الان بدنا .. برضه بدنا نعكس + +257 +00:19:31,730 --> 00:19:36,350 +الموضوع الفيروس اللى بيصيبنا احنا تمام؟ احنا + +258 +00:19:36,350 --> 00:19:39,670 +بيسموه animal viruses او human viruses تمام؟ ما + +259 +00:19:39,670 --> 00:19:43,270 +بتصيب النباتات؟ ما بتصيب النباتات؟ لإن مافيش اللي + +260 +00:19:43,270 --> 00:19:49,590 +هي receptors للنباتاتو هذا يسمى host specificity + +261 +00:19:49,590 --> 00:19:56,070 +يعني في نوع من التخصص ما بين ال microbe المعني و + +262 +00:19:56,070 --> 00:20:01,930 +ما بين ال host او بالظبط ال host يعني بصيب البط و + +263 +00:20:01,930 --> 00:20:05,730 +بصيب الحمام لكن مابصيبش الخنازير على سبيل المثال + +264 +00:20:05,730 --> 00:20:10,090 +او بصيب الخنازير و الخيل و ايك البط لكن مابصيبش + +265 +00:20:10,090 --> 00:20:14,990 +الحماممؤشر عليا على انه لا يحتوي ال receptor في + +266 +00:20:14,990 --> 00:20:17,290 +الحيوانات وهذا الكلام على فكرة بنشوفه في ال + +267 +00:20:17,290 --> 00:20:21,630 +influenza بنلاقي ال influenza بعض .. بعض السلالات + +268 +00:20:21,630 --> 00:20:26,930 +منها بتصيب الطيور وبعض السلالات بتصيب مثلا الخيل + +269 +00:20:26,930 --> 00:20:32,270 +البعض بتصيب الخنازير والبعض بتصيب خد بالك قد يحدث + +270 +00:20:32,270 --> 00:20:37,400 +ان يكون فيعامل مشترك مثلا اللي أجي فيروس influenza + +271 +00:20:37,400 --> 00:20:42,260 +بيصيب الخيل و الخنزير و واحد بيصيب الإنسان و + +272 +00:20:42,260 --> 00:20:45,440 +الخنزير لكن مابيصيبش الإنسان و الخيل في نفس الوقت، + +273 +00:20:45,440 --> 00:20:49,160 +مين العامل المشترك اللي اهان؟ الخنزير أكرمكم الله، + +274 +00:20:49,160 --> 00:20:53,470 +و هذا اللي بيحدث عندها تغيير تفرادإنه سلالتين + +275 +00:20:53,470 --> 00:20:56,790 +واحدة من الإنسان و واحدة من الخيل بيعملوا ال co + +276 +00:20:56,790 --> 00:21:00,630 +-infection في نفس اللحظة في الخنزير فقد يحدث تبادل + +277 +00:21:00,630 --> 00:21:05,610 +للجينات باسمها gene reassortment هدرسها لاحقا و ال + +278 +00:21:05,610 --> 00:21:09,030 +reassortment هدى عبارة عن استبدال قطع من المادة + +279 +00:21:09,030 --> 00:21:13,190 +الوراثية من كل من ال ال virusين و بيطلعنا سلالة + +280 +00:21:13,190 --> 00:21:19,690 +جديدة سلالة جديدةيبقى معنا الحكي انه بنفهم انه + +281 +00:21:19,690 --> 00:21:25,610 +عملية ال .. ال .. ال .. ال specificity تبع ال + +282 +00:21:25,610 --> 00:21:29,210 +receptor مرتبطة .. او افر ال .. ال .. ال .. ال + +283 +00:21:29,210 --> 00:21:33,090 +infection مرتبطة بوجود receptor للعوامل تبع ال + +284 +00:21:33,090 --> 00:21:36,310 +attachment تبع ال bacteria او اللي هو الفيروسة + +285 +00:21:36,310 --> 00:21:40,370 +واحنا بنقولكوا كمان مرة دائما و ابدا اول خطوة في + +286 +00:21:40,370 --> 00:21:42,670 +ال infection غالبا هي ال attachment + +287 +00:21:45,700 --> 00:21:48,780 +أنا قلت عن مين؟ عن ال species أو ال host + +288 +00:21:48,780 --> 00:21:53,220 +specificity، صح؟ طب داخل ال host الواحد، احنا زي + +289 +00:21:53,220 --> 00:21:57,980 +ما مثلا إنسان بشر، تراكيب الخلايا تبعته و الأنسجة + +290 +00:21:57,980 --> 00:22:01,280 +و الأعضاء مختلفة تماما، ال cardiac muscle غير عن + +291 +00:22:01,280 --> 00:22:03,280 +ال intestinal، الepithelium اللي موجود في + +292 +00:22:03,280 --> 00:22:06,420 +الانتستين غير عن الepithelium اللي موجود في ال + +293 +00:22:06,420 --> 00:22:11,670 +lungs، فهل هناكهل يكون هناك مجتمع عالمي على كل + +294 +00:22:11,670 --> 00:22:16,230 +الأنسجة؟ يمكن لأ ويمكن أ لأن في بعض المركبات لازم + +295 +00:22:16,230 --> 00:22:21,310 +تكون مشتركة بين كل الخلايا مثلًا مجتمع لا للوكوز + +296 +00:22:21,310 --> 00:22:22,070 +على سبيل المثال + +297 +00:22:25,800 --> 00:22:29,080 +إذا ماكانش على كل الأنثى لهذا الريسبتور اللى + +298 +00:22:29,080 --> 00:22:32,280 +بتستخدمه البتيرة في الإرتباط فمع ذلك الحكي إن هذا + +299 +00:22:32,280 --> 00:22:35,360 +الريسبتور هو اللى بيحدد ال organ أو ال tissue + +300 +00:22:35,360 --> 00:22:39,320 +tropism أو ال specificity كلمة tropism يعني ال + +301 +00:22:39,320 --> 00:22:43,180 +affinity affinity يعني القابلية للإرتباط، تمام؟ + +302 +00:22:43,180 --> 00:22:46,340 +ففي عندى شغلتين أنثى عندنا من ال adherence واحدة + +303 +00:22:46,340 --> 00:22:51,900 +متعلقة بال species specificity وتانية من dual + +304 +00:22:51,900 --> 00:22:55,000 +species اللى هو تيشيو تروبزم + +305 +00:22:58,370 --> 00:23:01,910 +طيب الجدول هذا اللي قدامكوا بنطلع عليه كويس لإن هو + +306 +00:23:01,910 --> 00:23:08,130 +اللي بوضح الفكرة تماما بتقرأ أسماء البكتيريا اللي + +307 +00:23:08,130 --> 00:23:15,370 +في أقصى اليسار و بعدين أطلع على العمود الأخير عشان + +308 +00:23:15,370 --> 00:23:20,430 +أوضح نقطة Striptococcus biogenes هذه رفيقة الدرب + +309 +00:23:20,430 --> 00:23:25,270 +تبعتكمتذكروا دائما انه يوم ما تنزل اللواز تصير + +310 +00:23:25,270 --> 00:23:28,710 +عندك الواز يعني انتهاد اللواز هيعرف انه هدى + +311 +00:23:28,710 --> 00:23:32,990 +streptococcus pyruids streptococcus mutants و ال + +312 +00:23:32,990 --> 00:23:36,590 +streptococcus salivaris هدولا تنتين موجودين في + +313 +00:23:36,590 --> 00:23:40,790 +اللعاب في القلعة الكبيرة ك normal flora ك normal + +314 +00:23:40,790 --> 00:23:46,970 +flora لكن ممكن أحداهم اللى هى الأولى ال mutants هي + +315 +00:23:46,970 --> 00:23:49,370 +تعمل dental caries تسوص الأصناف + +316 +00:23:52,850 --> 00:23:56,390 +يعني قاصد أن نتكلم على الأسماء بسرعة عشان نحنا + +317 +00:23:56,390 --> 00:24:00,230 +نشوف الأمراض ونشوف أماكن الأمراض هذه وإيش علاقة + +318 +00:24:00,230 --> 00:24:03,930 +الأدات بين الأمراض بالظبط هذي استفدت كوكاس + +319 +00:24:03,930 --> 00:24:08,310 +نيمونيا؟ أين موجودة؟ نيمونيا .. نيمونيا .. في + +320 +00:24:08,310 --> 00:24:11,970 +الرياض تاني، تمام استفدت كوكاس أوريا؟ هذه في كل + +321 +00:24:11,970 --> 00:24:16,950 +مكان، في كل مكان، تمام؟ إن سيريا جنورية؟ مرض جنسي + +322 +00:24:16,950 --> 00:24:21,850 +في الـUrogenital، في الـUrogenital tract طيباللوحة + +323 +00:24:21,850 --> 00:24:24,870 +اللي بعدها enterotoxinic escherichia coli في ال + +324 +00:24:24,870 --> 00:24:28,430 +intestine اللي بتعمل ديارية ال uropathionic + +325 +00:24:28,430 --> 00:24:31,510 +escherichia coli ممكن أنا مرتين لغرضين لكن واحدة + +326 +00:24:31,510 --> 00:24:34,170 +بتعمل urethritis واحدة بتعمل pionifritis وين ال + +327 +00:24:34,170 --> 00:24:37,610 +pionifritis؟ في الكيلية الكيلية بتنزل ال urine + +328 +00:24:37,610 --> 00:24:41,710 +تمام؟ فيه باستمرار washing ال urethritis ال + +329 +00:24:41,710 --> 00:24:45,030 +urethra اللي هي العضو الأخير في ال urinary tract + +330 +00:24:45,030 --> 00:24:49,880 +اللي بعد اللي هو ال bloodوبرضه الأمور عبره مين؟ ال + +331 +00:24:49,880 --> 00:24:53,940 +ball و ال ball بيعمل عملية washing تمام؟ ال + +332 +00:24:53,940 --> 00:24:57,720 +whopping cough في ال .. في ال lungs تمام؟ في + +333 +00:24:57,720 --> 00:25:00,220 +التراكيا اللي هي السعالة الديكي السعالة الديكي + +334 +00:25:00,220 --> 00:25:03,700 +اللي بسببها بوردوتيلا و Pertosis Vipariculera + +335 +00:25:03,700 --> 00:25:08,000 +دياريا tryponema pallidum syphilis مرض جنسي + +336 +00:25:08,000 --> 00:25:12,840 +Mycoplasma ليمونيا في ال lungs Klamydia + +337 +00:25:12,840 --> 00:25:19,320 +Conductivitis التعب المتحمة تبع تلعينor urethritis + +338 +00:25:19,320 --> 00:25:22,560 +زي ال extension كلها الأولانية، طب تعالوا نطلع على + +339 +00:25:22,560 --> 00:25:27,260 +الأماكن اللي احنا ذكرناها sore throat احنا بكونا + +340 +00:25:27,260 --> 00:25:33,760 +نلوازم لكن بنصومش، بمعنى بناكل و بنشرب، طب البلع + +341 +00:25:33,760 --> 00:25:37,500 +مش عملية ضغط على ال tonsils، هناك مش عارفين مش + +342 +00:25:37,500 --> 00:25:42,060 +عارفين القلم شديدمش الميكروبات المفروض إذا ماعنداش + +343 +00:25:42,060 --> 00:25:45,000 +المقدر على ال attachment وموجودة على ال .. تنزل + +344 +00:25:45,000 --> 00:25:48,800 +فيها تروح مع الأكل و تنزل على .. على المعنى و تموت + +345 +00:25:48,800 --> 00:25:52,400 +في المعنىلكن واقع الأمر هناك أنواع من الـ + +346 +00:25:52,400 --> 00:25:55,380 +tonsillitis لما بنطلّع عليها بنفتح الفم و بنشوفها + +347 +00:25:55,380 --> 00:25:59,920 +بنلاقي بُقع بيضاء على الـ tonsil نفسها عارفين شو + +348 +00:25:59,920 --> 00:26:03,280 +البُقع البيضاء هذي؟ مستعمرات striptococcus virus + +349 +00:26:03,280 --> 00:26:06,560 +مستعمرات دلالة على أن البكتيريا قادرة على ال + +350 +00:26:06,560 --> 00:26:11,240 +attach بشكل قوي جدا في هذا المكان طب نيجي لل + +351 +00:26:11,240 --> 00:26:16,400 +pneumonia احنا أول حارب نعملها لما بصيرنا + +352 +00:26:16,400 --> 00:26:20,220 +pneumonia بصير ننكحمظبوط؟ طب والكحة عبارة على + +353 +00:26:20,220 --> 00:26:24,660 +فعلين عن إيش؟ عن explosion من الهواء بيضغط .. + +354 +00:26:24,660 --> 00:26:27,740 +بيضغط .. بيضغط .. بيضغط فيه أمبوبرا فيها + +355 +00:26:27,740 --> 00:26:31,380 +الواتراكيا، يعني المفروض أن أي بكتيريا مع ال .. مع + +356 +00:26:31,380 --> 00:26:36,920 +القدائف إيش يحصل فيها، تطلع برا، ومع ذلك عند + +357 +00:26:36,920 --> 00:26:40,100 +المقدرة على attachment بتبقى ملازقة جنوبهاطب + +358 +00:26:40,100 --> 00:26:43,500 +عارفين التراكيا فيها ما يسمى بالـ Celia Reactions + +359 +00:26:43,500 --> 00:26:47,900 +Celia Reactions اللي هي حركة السيليا الأهداب حركة + +360 +00:26:47,900 --> 00:26:51,880 +السيليا هذه موجودة باستمرار 24 ساعة شغالة و + +361 +00:26:51,880 --> 00:26:56,180 +بيسموها المصعد ايش هي المصعد؟ أسانسير بنزل .. + +362 +00:26:56,180 --> 00:27:00,080 +بيجيب من تحت طلع وين؟ طلع فور إيش بيطلع؟ طلع + +363 +00:27:00,080 --> 00:27:05,320 +الميوكاس، المخاطب جسمنا أو ال lungs بتنتج ميوكاس + +364 +00:27:05,320 --> 00:27:07,900 +عشان تعمل treatment للغبرة و ال micro organism + +365 +00:27:09,630 --> 00:27:16,710 +فباستمرار ال lungs بتنتج ميوكاس بيرتبط بالغبار + +366 +00:27:16,710 --> 00:27:20,430 +و بال micro organism عشان ما يستقرش ال micro + +367 +00:27:20,430 --> 00:27:24,650 +organism بنطلع بره و بنتخلص منه عبر الكحة الخفيفة + +368 +00:27:24,650 --> 00:27:29,630 +في معظم الأحيان تخيلوا ان السيليا اللي بتتحرك هذه + +369 +00:27:29,630 --> 00:27:32,910 +الحركة المنتضمة فجأة واحد قد مسك السيليا هيك + +370 +00:27:39,870 --> 00:27:43,610 +إنه في بكتيريا بتجري تعمل attachment على السيريا، + +371 +00:27:43,610 --> 00:27:48,630 +تمام؟ فبصير في وزن وارتباط عدم انتظام في الحركة + +372 +00:27:48,630 --> 00:27:51,870 +فبصير في عندي accumulation في الbertosis في ال .. + +373 +00:27:51,870 --> 00:27:55,910 +اللي السعالة تديكي accumulation للبكتيريا و ال + +374 +00:27:55,910 --> 00:28:00,150 +mucus في منطقة محددة فبصير في عندي عملية السعالة + +375 +00:28:00,150 --> 00:28:03,530 +اللي هو اللي مستمر لفترة طويلة اللي بيقولنا عنه مش + +376 +00:28:03,530 --> 00:28:07,660 +بسعالةطيب الدياريا مش هنقول نشوفنا + +377 +00:28:07,660 --> 00:28:11,280 +انترولوكتكسوجينك إشريشة كلها و نشوفنا الفيبلو كلها + +378 +00:28:11,280 --> 00:28:14,960 +بتعمل إسهال و الإسهال عبارة عن برضه running water + +379 +00:28:14,960 --> 00:28:18,240 +running water مياه جارية مش مفروض تعمل flushing + +380 +00:28:18,240 --> 00:28:24,340 +للبكتيريا و مع ذلك البكتيريا تبقى هناك تتجمد + +381 +00:28:24,340 --> 00:28:27,640 +تعالوا نطلعوا على اللي بتسبب urinary tract أو + +382 +00:28:27,640 --> 00:28:30,720 +genitourinary tract طب ما ال urine يقولنا احنا + +383 +00:28:30,720 --> 00:28:35,010 +برضه عملية تنظيفومع ذلك البكتيريا تبقى هناك اذا + +384 +00:28:35,010 --> 00:28:39,470 +بدنا نطلع دعاين من الاحترام والتقدير على المركبات + +385 +00:28:39,470 --> 00:28:43,550 +اللي بتخلى البكتيريا تبقى في هذه الأماكن لإنها + +386 +00:28:43,550 --> 00:28:49,710 +المسؤولة طيب أعطيكم مثال على بي لاي اللي موجودة في + +387 +00:28:49,710 --> 00:28:57,990 +نشيليا جونورية وراحت أخر واحدة في القائمة الان + +388 +00:28:57,990 --> 00:29:04,040 +هذه البكتيريا هذه البكتيريارقم واحد فيها virulence + +389 +00:29:04,040 --> 00:29:08,260 +factor هو ال P-Ly هو ال P-Ly طب تخيلوا جسم الإنسان + +390 +00:29:08,260 --> 00:29:11,340 +هو ذكي برضه ال immune system تبقى على ذكي بيروح + +391 +00:29:11,340 --> 00:29:16,500 +بيكوّن antibody لمين؟ لل P-Ly عشان ترجعله وعارف + +392 +00:29:16,500 --> 00:29:20,400 +حالها بدون P-Ly عارف حالها بدون P-Ly غير قادرة على + +393 +00:29:20,400 --> 00:29:24,060 +إنها تبقى في هذا المكان فبتروح تعمل mutation في + +394 +00:29:24,060 --> 00:29:27,520 +مين؟ في ال P-Ly في الروتينة وبالتالي ترجع مرة + +395 +00:29:27,520 --> 00:29:32,780 +تاكتروصل الصورة يعني في ال attachment ثبت بالدليل + +396 +00:29:32,780 --> 00:29:36,980 +القاطع من اللوحة اللي قدامنا على الرغم من اختلاف + +397 +00:29:36,980 --> 00:29:41,200 +المركبات المستخدمة في ال attachment الا انه ال + +398 +00:29:41,200 --> 00:29:45,600 +attachment كغراب هي عامل مهم جدا في عاملته اللي هو + +399 +00:29:45,600 --> 00:29:49,700 +ال path Genesis وبالتالي هي viral as fuck وانا + +400 +00:29:49,700 --> 00:29:56,610 +اتفقنا انه اي عاملبيأثر إيجابا على ال pathogenesis + +401 +00:29:56,610 --> 00:30:03,810 +تبع البكتيريا تمام هو violence factor هذه الفكرة + +402 +00:30:03,810 --> 00:30:08,110 +العامة على ال addition أنه البكتيريا من خلال + +403 +00:30:08,110 --> 00:30:11,530 +الادهسين اللي أين من الأشياء الأخرى اللي ذكرناها + +404 +00:30:11,530 --> 00:30:15,250 +اسمه ادهسين بيرتبط على ال receptor اللي مولد في ال + +405 +00:30:15,250 --> 00:30:19,240 +epithelial cell سبعة أنسجات الإنسانمثل ال + +406 +00:30:19,240 --> 00:30:22,400 +Astrococcus biogenes كانت حاسمة protein F أو F + +407 +00:30:22,400 --> 00:30:26,240 +protein وبيرتبط مع الفيبرونكتين اللي موجود على بعض + +408 +00:30:26,240 --> 00:30:29,300 +ال epithelial cells و هذا ال binding قولنا binding + +409 +00:30:29,300 --> 00:30:33,960 +قوي و من خلاله البكتيريا تبقى هناك على الرغم من + +410 +00:30:33,960 --> 00:30:41,520 +انه في forces بتحاول انها تخلص منكالفيمبريا انا + +411 +00:30:41,520 --> 00:30:45,820 +ممكن في ال structure حاولت اني امر مرور سريع على + +412 +00:30:45,820 --> 00:30:49,220 +وظيفتين رئيسيتين في الفيمبريا او البيلاي و احنا + +413 +00:30:49,220 --> 00:30:51,660 +كنا الفيمبريا و البيلاي كانوا اسمين بيستخدموه + +414 +00:30:51,660 --> 00:30:55,400 +interchangeably بمعناه انهم بي .. مرات بيميزوش + +415 +00:30:55,400 --> 00:30:59,380 +بينهم أساسا لكن خصصوا ال .. البيلاي ال .. يسموها + +416 +00:30:59,380 --> 00:31:02,520 +ال six-pili و التانية جالو عنها attachment فيمبريا + +417 +00:31:02,520 --> 00:31:06,180 +لكن بواقع الأمر لحد دلان كتير من ال textbook + +418 +00:31:06,180 --> 00:31:10,200 +بتتكلم عنهم كانهم تركيب واحداللي انا بدأ أقوله + +419 +00:31:10,200 --> 00:31:13,760 +اليوم مختلف عن اللي قلته في السابق مش لإنه + +420 +00:31:13,760 --> 00:31:17,940 +حكيتلكوا علامات خطأ لأ ماحبناش نعمل confusion في + +421 +00:31:17,940 --> 00:31:21,660 +البداية بس الآن لازم نقولكوا الحقيقة المرة إنه + +422 +00:31:21,660 --> 00:31:26,780 +البيلاي و الفيمبريا ليست بهذه البساطة اللتي + +423 +00:31:26,780 --> 00:31:32,040 +درسناها يعني بمعنى أخر ممكن تلاقوا textbook كامل + +424 +00:31:32,040 --> 00:31:39,550 +عن البيلاي و الفيمبريابيشمل مؤتمرات مظلمة فقط + +425 +00:31:39,550 --> 00:31:43,990 +لدراسة البيلاي والفملي طبعا للي أكيد لهميتها و أنا + +426 +00:31:43,990 --> 00:31:47,810 +قاعد بتكلم إلي ربع ساعة عن ال attachment و أهمية + +427 +00:31:47,810 --> 00:31:51,070 +ال attachment فيه مش بس في ال .. في ال .. في ال + +428 +00:31:51,070 --> 00:31:54,630 +pathogenesis تخيلوا في المستشفيات البكتيرا اللي + +429 +00:31:54,630 --> 00:31:58,170 +عندها قدرة على ال attachment مع الأسطح قد تكون + +430 +00:31:58,170 --> 00:32:03,230 +يسمى biofilms إيش يعني bio؟ films طبقات بيولوجية + +431 +00:32:04,040 --> 00:32:07,140 +طيب أقولكوا إيش يعني biofilm عشان تقدروا الموضوع + +432 +00:32:07,140 --> 00:32:09,360 +ال attachment طبعا ال biofilm كلكوا شفتهوا في + +433 +00:32:09,360 --> 00:32:13,780 +الدار لما عملته مخلة لزيتون و عفن طلع فوق هذا + +434 +00:32:13,780 --> 00:32:17,700 +الأبيض و المخلة الخيار هذا اسمه biofilm ليش + +435 +00:32:17,700 --> 00:32:22,000 +البكتيريا طاع فيه على سطر هذه بكتيريا لأن هي عندها + +436 +00:32:22,000 --> 00:32:25,020 +المقدرة على ال attachment بينها و بين بعضها لكن + +437 +00:32:25,020 --> 00:32:27,780 +هناك بكتيريا عندها المقدرة على ال attachment بينها + +438 +00:32:27,780 --> 00:32:32,640 +و بين الأسطح تخيلوا يا ابنات مكينة غذاء بنقطع فيها + +439 +00:32:32,640 --> 00:32:37,990 +غذاءبقايا بيضل شوية بقايا فبتجي البكتيريا بتنمو + +440 +00:32:37,990 --> 00:32:40,950 +لما تنمو بتروح اللي هي ملازجة في الماكينة نفسها + +441 +00:32:40,950 --> 00:32:45,230 +ايه طبعا بنغسلها بنرشها بالمي احنا في اماكن يصعب + +442 +00:32:45,230 --> 00:32:49,970 +عينا الوصول الفرشاية فبتكون هناك هذه البكتيريا إذا + +443 +00:32:49,970 --> 00:32:54,180 +.. إذا من النوع اللي بيكون biofilmحتى لو حطينا + +444 +00:32:54,180 --> 00:32:58,620 +عليها كلور خد بالكوا البكتيريا ال behavior تبعها + +445 +00:32:58,620 --> 00:33:03,980 +لما بتكون single cells خلايا منفصلة بيختلف تماما + +446 +00:33:03,980 --> 00:33:08,860 +عن ما لو كانت biofilm يعني لو كنا نقتلها بتركيز 3% + +447 +00:33:08,860 --> 00:33:14,700 +كلور تمام بدنا 6% كلور لل biofilm او 10% كلورين لل + +448 +00:33:14,700 --> 00:33:18,500 +biofilm ال behavior تبع ال biofilm بيختلف احد + +449 +00:33:18,500 --> 00:33:22,850 +مظاهر الاختلاف هو its ability to resistresist + +450 +00:33:22,850 --> 00:33:25,930 +what? different methods of disinfection and + +451 +00:33:25,930 --> 00:33:29,370 +sterilization طب الكلام اللي بحكيه أنا هذا ما هو + +452 +00:33:29,370 --> 00:33:32,330 +نفسه بينطبق على المستشفى لما أنا أركب canula + +453 +00:33:32,330 --> 00:33:36,370 +للمريض ال canula وإن كانت مصموعة من ال plastic + +454 +00:33:36,370 --> 00:33:40,210 +تمام و ال plastic ناعم و هي معقمة و تمام تمام إلا + +455 +00:33:40,210 --> 00:33:45,850 +أنه بالنسبة للبكتيريا هي جسم خشن جدا بالنسبة لحجم + +456 +00:33:45,850 --> 00:33:50,590 +البكتيريا خشن جدا و تستطيع أن تبقى ملتصقا فيه + +457 +00:33:51,680 --> 00:33:54,820 +تعرفوا انتوا مرضى الشلل الرابعى او النصف اللى + +458 +00:33:54,820 --> 00:33:59,540 +بيصير انه شلل بعد نتيجة حادث او كده طبعا بيصيروا + +459 +00:33:59,540 --> 00:34:03,100 +يفقدوا السيطرة على التبول فبيركبولهم قاسترة بولية + +460 +00:34:03,100 --> 00:34:06,960 +قاسترة بولية اللى بيدخل انبوب الى داخل المثارة عبر + +461 +00:34:06,960 --> 00:34:12,220 +اليوريثرة سواء male او female هلاجى انه الشخص هذا + +462 +00:34:12,220 --> 00:34:16,480 +اللى دخلناله قاسترة صار عنده urinary tract + +463 +00:34:16,480 --> 00:34:21,950 +infection باستمرارليش؟ لأنه القسطرة .. الجزء اللي + +464 +00:34:21,950 --> 00:34:27,650 +دخل جوا من القسطرة أصبح matrix، يعني مكان لل + +465 +00:34:27,650 --> 00:34:31,270 +biofilm للبكتيريا، بنعالجه، بنقتل البكتيريا اللي + +466 +00:34:31,270 --> 00:34:34,930 +كانت single cells لحالها، اللي عاملة infection، + +467 +00:34:34,930 --> 00:34:37,710 +لكن لا انا استطيع اقتل مين؟ ال biofilm اللي موجود + +468 +00:34:37,710 --> 00:34:40,770 +طب بنموت البكتيريا، بنطيب المريض، بعد يوم أو + +469 +00:34:40,770 --> 00:34:43,370 +يومين، بنتوقع من إيقاف ال antibiotic، بيرجع مرة + +470 +00:34:43,370 --> 00:34:48,550 +أخرى ال infectionخلصت الموضوع، لو جعدنا مئة سنة + +471 +00:34:48,550 --> 00:34:52,230 +نتكلم عن ال attachment، هنلاقي مواضيع نتكلم فيها، + +472 +00:34:52,230 --> 00:34:57,350 +من أهمية هذا الموضوع، إذن ال attachment سواء كان + +473 +00:34:57,350 --> 00:35:00,450 +على مستوى ال pathogenesis أو على مستوى ال + +474 +00:35:00,450 --> 00:35:03,490 +survival، و ال survival أنا فهمني في المستشفى، أنا + +475 +00:35:03,490 --> 00:35:06,070 +الدرس الماضي كان عن ال disinfection و ال + +476 +00:35:06,070 --> 00:35:08,930 +sterilization، مظبوط ولا لأ؟إذا ماكانش هندي + +477 +00:35:08,930 --> 00:35:12,670 +المقدرة بتعطيكوا مثال واقي من أبحاثنا اللي عملناها + +478 +00:35:12,670 --> 00:35:15,450 +كدراسة مجموعة من طالبات قسم الأعلام الطبية + +479 +00:35:15,450 --> 00:35:19,470 +المخبرية عملوا دراسة على المياه اللي بتشربوها في + +480 +00:35:19,470 --> 00:35:22,810 +الجامعة اللي بتلاقوها في الحنافيات أو ال boxes هذه + +481 +00:35:24,380 --> 00:35:28,480 +طبعا مصدرها واحد احنا خدنا من جهنم مصدر بلدية + +482 +00:35:28,480 --> 00:35:31,600 +وقعنا مصدر ال filter وقعنا مصدر بيئر .. بيئر خاص + +483 +00:35:31,600 --> 00:35:34,700 +بالجامعة واخدوا عينات من كل الأماكن المهم خلينا في + +484 +00:35:34,700 --> 00:35:37,780 +موضوعنا المياه اللي انتوا تشربوا منها اللي هي جام + +485 +00:35:37,780 --> 00:35:43,000 +الفلتر لجينا ان الفلتر بعطي ميه كويسة لكن لجينا + +486 +00:35:43,000 --> 00:35:46,740 +بعض ال .. الوحدات ال terminals تمام مابتعطيش ميه + +487 +00:35:46,740 --> 00:35:50,940 +كويسة فيها micro organismsفأطول بقتصرنا في شغل + +488 +00:35:50,940 --> 00:35:53,940 +إدارية وقلنا لهم يا جماعة المياه اللي عندنا في + +489 +00:35:53,940 --> 00:35:56,540 +الجامعة في بعض الأماكن واحد اتنين تلاتة أربع عملهم + +490 +00:35:56,540 --> 00:36:00,120 +زي خارطة انه يجب عليك تتوقفوا ال system يوم الخميس + +491 +00:36:00,120 --> 00:36:04,420 +و الجمعة يصير في الخميس او الأربعة و الخميس و + +492 +00:36:04,420 --> 00:36:08,200 +الجمعة و يصير this disinfection و الجماعة الصحيح + +493 +00:36:08,200 --> 00:36:10,720 +كانوا متعاونين جدا الإخوة في شغل إدارية و جاموا + +494 +00:36:10,720 --> 00:36:14,560 +بحملة رائعة جدا و نظفوا كل الأماكن مثل البنات يوم + +495 +00:36:14,560 --> 00:36:17,120 +السبت وروحوا خدوا عينات هتلاقوا البكتينة أكتر من + +496 +00:36:17,120 --> 00:36:17,320 +دقيقة + +497 +00:36:20,370 --> 00:36:23,250 +جمعوا عينات بالفعل لو البكتيريا عددها أكبر من ذي + +498 +00:36:23,250 --> 00:36:29,010 +قبل، إيش اللي صار؟ إنه الأماكن هذه اللي عملوا فيها + +499 +00:36:29,010 --> 00:36:33,370 +عملية تنظيف هم أثار ال biofilms، يعني عملوا تنظيف + +500 +00:36:33,370 --> 00:36:36,630 +فال biofilm اللي كانت راكدة وتعطي عدد معين من + +501 +00:36:36,630 --> 00:36:41,410 +البكتيريا باستمرار، مش استفزوها، أثروها بمعنى إن + +502 +00:36:41,410 --> 00:36:45,210 +هم بنفسهم أزقوهافتمظيقها الآن صار مورد الآن + +503 +00:36:45,210 --> 00:36:48,190 +بالسائق، فقالوا مضط في ال system كمان مرة وضخوا + +504 +00:36:48,190 --> 00:36:51,230 +مياه سخنة في ال system وضخوا مياه .. او chlorine + +505 +00:36:51,230 --> 00:36:53,590 +dosage جولة عالية و بعدين يغسلوا ال system و + +506 +00:36:53,590 --> 00:36:57,410 +بالفعل صار عملية التنظيف، القصد من الكلام أنه + +507 +00:36:57,410 --> 00:37:01,070 +طريقة الت .. الت .. التنظيف اختلفت لإن البكتيريا + +508 +00:37:01,070 --> 00:37:05,610 +كانت عاملة ايه؟ بيوم، اه دي نقطة كتير، يبقى ال + +509 +00:37:05,610 --> 00:37:06,550 +attachment بنات + +510 +00:37:09,760 --> 00:37:14,240 +ميزة للبكتيريا إذا انتلقتها .. انتلقت يعني صفات + +511 +00:37:14,240 --> 00:37:21,420 +إيجابية إلها تؤهلها لل survival بشكل جيد طيب + +512 +00:37:21,420 --> 00:37:24,980 +النقطة التانية اللي بعد ال attachment اللي هي ال + +513 +00:37:24,980 --> 00:37:29,360 +invasion of host cell antigen اتفقنا إنه في + +514 +00:37:29,360 --> 00:37:33,840 +إمكانية لل invasion أو localized infection خلنا + +515 +00:37:33,840 --> 00:37:36,660 +نجسم ال infection اسمه .. نسميها localized و + +516 +00:37:36,660 --> 00:37:40,460 +systemic إيش يعني localized؟يعني محددة في local + +517 +00:37:40,460 --> 00:37:44,300 +area معينة زي التيتانوس ما كنا نقول أصعب منطقة من + +518 +00:37:44,300 --> 00:37:47,900 +الجلب أو من العضل و بقية هناك بينما السايمونيلا + +519 +00:37:47,900 --> 00:37:52,000 +تايفي انتقلت من ال intestine إلى ال blood إلى ال + +520 +00:37:52,000 --> 00:37:55,760 +gallbladder إلى ال ال central nervous system و هذا + +521 +00:37:55,760 --> 00:38:00,420 +زمناها systemic ما الذي يؤهل البكتيريا إنها تعمل + +522 +00:38:00,420 --> 00:38:01,440 +invasion؟ + +523 +00:38:06,440 --> 00:38:13,660 +لكن لا بأس إننا نتطلع على المسميات وبعدين نناقش ال + +524 +00:38:13,660 --> 00:38:19,120 +function تبع كل مسمى منهم الاسم الأول + +525 +00:38:19,120 --> 00:38:24,420 +Hyaluronidase، الاسم التاني collagenase، الاسم + +526 +00:38:24,420 --> 00:38:29,180 +التالف neuroaminidase، الرابع coagulase، الخانس + +527 +00:38:29,180 --> 00:38:34,720 +kinase، لاحظوا الشغل؟كلهم aces يعني enzymes وكل + +528 +00:38:34,720 --> 00:38:39,000 +واحد اسمه معاه اسمه معاه coagulation بيعمل + +529 +00:38:39,000 --> 00:38:44,700 +coagulation ال kinases مابتعرفوهاش بتحلل نعم بتحلل + +530 +00:38:44,700 --> 00:38:50,460 +ال clots عكس ال coagulase بره لابن الجرح كيف الرسم + +531 +00:38:50,460 --> 00:38:53,700 +بيعمل healing؟ أحد بيعمل ال clotting بيعمل ال + +532 +00:38:53,700 --> 00:38:57,620 +clotting مظبوط ولا لأ؟ تخيلوا لو بكتيريا من النوع + +533 +00:38:57,620 --> 00:39:02,500 +اللي بنتش kinase قلت و استقرت في الجرحكل ما يتكوّن + +534 +00:39:02,500 --> 00:39:07,460 +الكلتش هتعملها .. هتعمله عملية تحليل للجلطة على + +535 +00:39:07,460 --> 00:39:11,000 +فكرة هذه استخدامات ظاهرة في علاج أمراض الجلطة طبعا + +536 +00:39:11,000 --> 00:39:14,060 +هبيروح على المستشفى على مرض القلب يلاقي عندهم علاج + +537 +00:39:14,060 --> 00:39:17,860 +غير ثمن جدا غير اسمه streptokinase streptokinase + +538 +00:39:17,860 --> 00:39:21,720 +ماخود من streptococcus من streptococcus حينك سموه + +539 +00:39:21,720 --> 00:39:25,480 +streptokinase في استفيدوا كينيز لكن لا يستخدم لا + +540 +00:39:25,480 --> 00:39:31,120 +يستخدمال coagulase .. ال coagulase بيحول ال + +541 +00:39:31,120 --> 00:39:38,100 +fibrinogen إلى fibrin عكس اللي هو ال kinase تخيلوا + +542 +00:39:38,100 --> 00:39:41,860 +ال Staphylococcus aureus تنتج coagulase و الموضوع + +543 +00:39:41,860 --> 00:39:45,440 +ده في دمنا و كل ما جاب ال fibrinogen بتحوله ل + +544 +00:39:45,440 --> 00:39:49,400 +fibrin يعني هتعمل clots في الجسم، هتعمل جلطات في + +545 +00:39:49,400 --> 00:39:53,180 +الجسمطبعا هي .. هي مش قصدها انها تقتل ال host في + +546 +00:39:53,180 --> 00:39:55,920 +الإيطار و انما قصدها تحمي نفسها .. احنا بدنا ندافع + +547 +00:39:55,920 --> 00:39:59,320 +عن البكتيريا .. احنا الكل بي .. الكل ضدها .. احنا + +548 +00:39:59,320 --> 00:40:02,060 +علىقل نكون يعني من الناس اللي بيدافع عن البكتيريا + +549 +00:40:02,060 --> 00:40:06,000 +.. هي تقصد انها تحمي نفسها من اللي هو ال immune + +550 +00:40:06,000 --> 00:40:09,940 +system تبع الإنسان انها تكون داخل شيء لزج .. داخل + +551 +00:40:09,940 --> 00:40:13,680 +شيء لزج اللي هو ال blood فهو بيحميها من ال immune + +552 +00:40:13,680 --> 00:40:17,520 +system .. لكن دون أن تقصد قد تقتلنا .. مافي مشكلة + +553 +00:40:17,520 --> 00:40:20,280 +.. يعني مبدأ مش دون قصد .. يعني ال D تبعت غير .. + +554 +00:40:20,550 --> 00:40:26,190 +الدية تبعتها رهيب طيب .. طب مين .. مين .. مين + +555 +00:40:26,190 --> 00:40:29,590 +بالكه أنجح؟ ال host .. اه عفوا .. ال .. ال .. ال + +556 +00:40:29,590 --> 00:40:34,910 +.. ال pathogen القاتل ولا ال pathogen اللي ممكن + +557 +00:40:34,910 --> 00:40:40,330 +يعيش معانا بدون ما يقتلنا؟ انه أنجح؟ من وجه نظر .. + +558 +00:40:40,330 --> 00:40:43,390 +من وجه نظر ال pathogen نفسه؟ انه أحسنله؟ + +559 +00:40:45,860 --> 00:40:51,000 +يعني بما ناخر بيسكتر و بيبقينا حيان لإنه هيطيل من + +560 +00:40:51,000 --> 00:40:56,400 +فترة حياته هو، بيطل فترة حياتنا احنا، أما لو قتلنا + +561 +00:40:56,400 --> 00:41:00,240 +تمام زي ال anthrax فهو .. احنا هنموت لإن هو مش + +562 +00:41:00,240 --> 00:41:03,960 +هلاقي غضب و كده ليه هيموت، إذا للأنجح إله إنه .. + +563 +00:41:04,250 --> 00:41:08,970 +يبقى زي .. زي ال .. البكتيروفاج لما دخل الخلية و + +564 +00:41:08,970 --> 00:41:13,310 +جالهم انا بس ماعليش يعني .. مؤقتا يعني .. اه و + +565 +00:41:13,310 --> 00:41:16,950 +انقسم من قسام الخلية، هذا كان أنجح من مين؟ من أخوه + +566 +00:41:16,950 --> 00:41:23,470 +اللي دخل و حلل الخلية بشكل عنيف فين حالة اسمها + +567 +00:41:23,470 --> 00:41:27,210 +streptomycin؟ اعفوا، ليوكوسايدين، ليوكوسايدين + +568 +00:41:27,210 --> 00:41:31,910 +ليوكوسايدين كلمة .. سايدين معناها .. من جاية من + +569 +00:41:31,910 --> 00:41:37,020 +كلمة سايدل، سايدل القتلاليوكو أبيض القاتل الأبيض + +570 +00:41:37,020 --> 00:41:40,940 +يعني مالأي قاتل كورات الدم البيضاء قاتل لكورات + +571 +00:41:40,940 --> 00:41:45,160 +الدم البيضاء هذا متخصص البكتيريا تنتجه عشان يقتل + +572 +00:41:45,160 --> 00:41:48,020 +كورات الدم البيضاء عشان نعرف ان كورات الدم البيضاء + +573 +00:41:48,020 --> 00:41:54,280 +المصبوط ال macro phage و ال phagocytes كلها من + +574 +00:41:54,280 --> 00:41:58,140 +اللي تنتمي إلى ال white blood cell فلما نلاقي خلية + +575 +00:41:58,140 --> 00:42:02,320 +قادرة على قتل ال white blood cell هذا مؤشر يعني + +576 +00:42:02,320 --> 00:42:06,360 +أنه البكتيرياعندها virulence factor J نيجي لل + +577 +00:42:06,360 --> 00:42:09,200 +Striptolysin والهيموليسين الصحيح أنه .. يعني + +578 +00:42:09,200 --> 00:42:13,220 +الهيموليسين أشمل لإن ال Striptolysin هو هيموليسين + +579 +00:42:13,220 --> 00:42:17,480 +بس تنتجه اللي هو ال Striptococcus يعني متخصص بال + +580 +00:42:17,480 --> 00:42:22,560 +Striptococcus هيموليسين بيعمل هيموليس لمين؟ لل .. + +581 +00:42:22,560 --> 00:42:26,080 +هو لل Red Blood Cell أكتر شي عشان يسموه Hemo .. + +582 +00:42:26,080 --> 00:42:30,870 +Hemo و ال Hem في ال Red Blood Cellطبعا + +583 +00:42:30,870 --> 00:42:33,670 +الستربتولاسين هو Hemolysin بانجام مرة بس بتنتج + +584 +00:42:33,670 --> 00:42:38,390 +لستربتو كوكس لسه تنايز و phospholipases برضه نفس + +585 +00:42:38,390 --> 00:42:43,530 +الفكرة ال .. ال .. ال .. ال phospholipases اسم عام + +586 +00:42:43,530 --> 00:42:46,910 +لمجموعة من الانزيمات قادرة على تحليل ال + +587 +00:42:46,910 --> 00:42:50,330 +phospholipidsأحد ال phospholips المشهورة اللي هو + +588 +00:42:50,330 --> 00:42:54,990 +ال licitin بتنتجه بكتيريا زي كلوسترديوم بنسميه + +589 +00:42:54,990 --> 00:43:00,150 +licitinase واسم تاني حتى phospholipase C كده ال + +590 +00:43:00,150 --> 00:43:04,330 +licitin أو عفوا licitinases هي تبع لمين؟ لل + +591 +00:43:04,330 --> 00:43:10,460 +phospholipaseفي عندنا anthrax enema factor و + +592 +00:43:10,460 --> 00:43:15,900 +pertosis adenylate cyclase enzyme AC اختصار ال + +593 +00:43:15,900 --> 00:43:20,020 +adenylate cyclase enzyme ناخد الأخير adenylate + +594 +00:43:20,020 --> 00:43:23,660 +cyclase enzyme بتعرفوا حاجة اسمها Cyclic A and B؟ + +595 +00:43:23,660 --> 00:43:29,500 +Cyclic A and B طيب بتعرفوا حاجة اسمها ATP؟ ال + +596 +00:43:29,500 --> 00:43:34,900 +Cyclic A and B أحد منتجات اللي هو ال ATPخد بالك ال + +597 +00:43:34,900 --> 00:43:39,700 +Cyclic AMP الوظائف رائعة جدا في الجسم ومحددة + +598 +00:43:39,700 --> 00:43:47,640 +وكميته على قدر وظيفته إذا زادت كميته زادت وظيفته + +599 +00:43:47,640 --> 00:43:52,620 +ومشكلة إنه تزيد الوظيفة أنا بدي أنتج عشر مركبات في + +600 +00:43:52,620 --> 00:43:56,920 +اليوم هاي طاقة الإنتاجية وبيجوبوا بالمهمة تماما + +601 +00:43:56,920 --> 00:43:58,300 +عشرة لو أنتجت مية + +602 +00:44:01,530 --> 00:44:04,590 +يبقى مانع أكثر في خلل في ال system اللي أنا مخططه + +603 +00:44:04,590 --> 00:44:09,490 +علشان أن أنت فقط عشان ال adenylate cyclase enzyme + +604 +00:44:09,490 --> 00:44:15,850 +بيعمل نشاط معين يؤدي هذا النشاط إلى إنتاج كمانية + +605 +00:44:15,850 --> 00:44:20,290 +كبيرة من من؟ من ال cyclic AMP فال cyclic AMP بدل + +606 +00:44:20,290 --> 00:44:22,950 +ما يجم بالوظيفة المحدودة للجدي صارت جم بالوظيفة + +607 +00:44:22,950 --> 00:44:28,000 +كبيرة فتؤدي إلى خلل كلمة خلل ما أنتوا عارفين؟عطيكم + +608 +00:44:28,000 --> 00:44:30,620 +مثال على أديناليت سايكليز أنزايم ال Vibrio cholera + +609 +00:44:30,620 --> 00:44:35,820 +بتنتج cholera تكسين لو وصفناه احنا بنوصفه على أنه + +610 +00:44:35,820 --> 00:44:41,640 +أديناليت سايكليز أنزايم بيرتبط التكسين بالأديناليت + +611 +00:44:41,640 --> 00:44:46,420 +سايكليز بيعمله activation لما يصير activation + +612 +00:44:46,420 --> 00:44:51,240 +للأديناليت سايكليز أنزايم بيتحول ال ATP لcyclic + +613 +00:44:51,240 --> 00:44:54,620 +AMP يبقى صرفين ينتج كمية كبيرة من مين؟ من ال + +614 +00:44:54,620 --> 00:44:59,260 +cyclic AMPالانستكليك EMB بيمنع ال absorption، + +615 +00:44:59,260 --> 00:45:02,340 +مظيفته يمنع ال absorption تبع ال chloride و يزيد + +616 +00:45:02,340 --> 00:45:05,800 +من ال secretion تبع ايه؟ الصوديوم، لما أصير في + +617 +00:45:05,800 --> 00:45:10,020 +عندي صوديوم في هذه الأمعاء، تخيلوا الأمعاء مصورة، + +618 +00:45:10,020 --> 00:45:13,640 +صار عندي عدم امتصاص لل chloride و إنتاج لمين؟ لل + +619 +00:45:13,640 --> 00:45:17,820 +sodium، فصار عندي كمية كبيرة من ال chloride جوا و + +620 +00:45:17,820 --> 00:45:22,400 +ال sodium، ال chloride و ال sodium و ال calcium، + +621 +00:45:22,400 --> 00:45:25,910 +هدول عبارة عن electrolytesإذا زادت كمية الـ + +622 +00:45:25,910 --> 00:45:29,070 +electrolyte في الـ human of the intestine الجسم + +623 +00:45:29,070 --> 00:45:34,030 +بيشعر إن هذا خطر يهدد حياة الإنسان فبروح بينتج + +624 +00:45:34,030 --> 00:45:37,630 +كمية كبيرة من إيه؟ من السائل اللي هو الميه to + +625 +00:45:37,630 --> 00:45:42,890 +dilute لإخفف دياريا is produced وصلت الصورة + +626 +00:45:42,890 --> 00:45:48,630 +فتخيلوا إن هذا الانيليزا غليز انزايم مش موجود له + +627 +00:45:48,630 --> 00:45:52,370 +تجويف في المكان اللي هو فيه زي الpertosis مثلا، + +628 +00:45:52,370 --> 00:45:57,130 +تمام؟الحقيقة ماتقلش الآن ما يسمى بالادمة اللي هي + +629 +00:45:57,130 --> 00:46:02,390 +الانتفاق في الاستثقاء اللي هو انه ميه تنزل في + +630 +00:46:02,390 --> 00:46:05,330 +الأنسجة extra cellular water لأنه برضه عملية + +631 +00:46:05,330 --> 00:46:09,130 +dilution لمين ده ال Electrolyte المرة القادمة ان + +632 +00:46:09,130 --> 00:46:14,210 +شاء الله بنأكد على بعض الزيادة تبع ال virus factor + +633 +00:46:14,210 --> 00:46:17,790 +هذا اللي هنتكلم فيه و بعض الناس هياخد معانا فترة + +634 +00:46:17,790 --> 00:46:22,640 +طويلةاللي بناخده في أول المحاضرة هيزمها في + +635 +00:46:22,640 --> 00:46:24,440 +المحاضرة التانية أو بالتالتة أو بالرابعة أو + +636 +00:46:24,440 --> 00:46:27,340 +بالخمسة فانا أرجو من حضراتكم ان انتوا تقراوا معانا + +637 +00:46:27,340 --> 00:46:27,740 +باسم الله + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/gejJKbVhKOc.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/gejJKbVhKOc.srt new file mode 100644 index 0000000000000000000000000000000000000000..eaf5dbe0f44f17adf2af5560e478e9bc2a8ca05d --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/gejJKbVhKOc.srt @@ -0,0 +1,2151 @@ +1 +00:00:21,720 --> 00:00:23,480 +أهلا و مرحبا الحمد لله ورحمة الله وبركاته و الصلاة والسلام + +2 +00:00:23,480 --> 00:00:26,500 +على سيدنا محمد وعلى آله وصحبه أجمعين + +3 +00:00:26,500 --> 00:00:30,740 +اليوم إن شاء الله أما بعد هذه المحاضرة هي الأخيرة في + +4 +00:00:30,740 --> 00:00:35,020 +الجزء المتعلق بالـ Viral Genes والـ Immunity لل + +5 +00:00:35,020 --> 00:00:39,100 +bacterial infection هنتكلم اليوم عن الـ Origin and + +6 +00:00:39,100 --> 00:00:43,630 +Development of Bacterial Infections في هنا مصطلحين + +7 +00:00:43,630 --> 00:00:47,530 +هنطلع عليهم بشكل دقيق المصطلح الأول بيقول إن ال + +8 +00:00:47,530 --> 00:00:51,770 +Sources of Infection إما Indigenous أو Exogenous + +9 +00:00:51,770 --> 00:00:55,530 +يعني يا إما مصدر داخلي يا إما مصدر خارجي من أين + +10 +00:00:55,530 --> 00:00:59,310 +اتّقت المصادر الداخلية للعدوى من الـ Normal + +11 +00:00:59,310 --> 00:01:03,830 +Microbiota اللي مولودة عندنا كلكم تتذكروا إن احنا + +12 +00:01:03,830 --> 00:01:08,090 +قسمنا الجسم إلى تسمية استرال ونون استرال كل الأجزاء + +13 +00:01:08,090 --> 00:01:13,270 +اللي نون استرال زي الـ Gastrointestinal tract تمام؟ ده ال + +14 +00:01:13,270 --> 00:01:16,970 +Vagina هذه تعتبر والـ Skin تعتبر Sources of + +15 +00:01:16,970 --> 00:01:22,370 +Indigenous اللي هو Infection أما الـ Exogenous هي + +16 +00:01:22,370 --> 00:01:26,430 +الكثيرة والمتنوعة اللي مثلًا على سبيل المثال ممكن + +17 +00:01:26,430 --> 00:01:30,570 +ناخدها من الـ Environment من الـ Air من الـ Surfaces + +18 +00:01:30,570 --> 00:01:35,470 +تمام؟ من الـ Close Contact مع الآخرين من الماء اللي + +19 +00:01:35,470 --> 00:01:38,290 +بنتناوله أو من الغذاء اللي احنا بنتناوله ده + +20 +00:01:38,290 --> 00:01:39,210 +بيسميها إيه؟ + +21 +00:01:45,230 --> 00:01:47,470 +الـ Cycle دا يا عزيزي هي الـ Cycle نريد أن نفهمها ون + +22 +00:01:47,470 --> 00:01:56,390 +نرجع لها طول فترة الحديث الـ Cycle بتلخص لنا سلسلة + +23 +00:01:56,390 --> 00:02:01,870 +الأحداث المتعلقة بأي Infection في الدنيا والسلسلة + +24 +00:02:01,870 --> 00:02:03,550 +دي اللي عملتها باللغة العربية اللي عملتها في + +25 +00:02:03,550 --> 00:02:07,530 +محاضرة أعتقد لمستوى أقل من مستواكم لكن في الآخر هي + +26 +00:02:07,530 --> 00:02:11,490 +تعبر عن الواقع الـ .. الـ .. الـ .. Infection Cycle + +27 +00:02:11,490 --> 00:02:14,350 +بنبدأ بالعامل المسبب + +28 +00:02:18,370 --> 00:02:23,850 +قد يكون بكتيريا قد يكون فيروس قد يكون فنجي قد يكون + +29 +00:02:23,850 --> 00:02:28,010 +برزيّت والبرزيّت قد تكون ديدان أو قد تكون بروتوزوا + +30 +00:02:28,010 --> 00:02:33,090 +لكن في الآخر هي الـ Pathogens الآن أي Pathogen في + +31 +00:02:33,090 --> 00:02:35,390 +الدنيا له ما يسمى بالـ Reservoir والـ Reservoir + +32 +00:02:35,390 --> 00:02:40,750 +يعني مخازن مخزن للعدوى أعطيكم مثال على مخازن يعني + +33 +00:02:40,750 --> 00:02:46,410 +إيش يعني مخازن للعدوى إنسان مصاب بفيروس الرشح هو + +34 +00:02:46,410 --> 00:02:49,930 +مخزن لفيروس الرشح لأنه كل ما بيعطس هو بيطلع + +35 +00:02:49,930 --> 00:02:55,170 +الفيروس فهو مخزن للعدوى تمام؟ قد يكون المخزن + +36 +00:02:55,170 --> 00:03:00,350 +الإنسان المصاب، قد يكون الإنسان الحامل للمرض مخزن، + +37 +00:03:00,350 --> 00:03:03,430 +قد تكون بعض الحشرات مخزن، قد تكون البيئة نفسها + +38 +00:03:03,430 --> 00:03:08,450 +مخزن، قد تكون حيوان آخر مخزن، يبقى فيها إنها مخزن، + +39 +00:03:08,450 --> 00:03:14,100 +بس إيش هو مرتبط بمين؟ باسم الـ Pathogen نفسه الآن + +40 +00:03:14,100 --> 00:03:17,980 +احنا قلنا إن الإنسان المصاب مخزن صح ولا لأ عشان ال + +41 +00:03:17,980 --> 00:03:22,420 +Infection تحدث لازم يخرج الميكروب من المخزن لازم + +42 +00:03:22,420 --> 00:03:27,820 +يكون له Portal of Exit مكان خروج تبقى أماكن الخروج + +43 +00:03:27,820 --> 00:03:33,300 +زي مثلًا زي الـ Secretions الصديدية + +44 +00:03:33,300 --> 00:03:36,300 +على سبيل المثال واحد عنده دمل مش هذا Infection وال + +45 +00:03:36,300 --> 00:03:42,240 +Pathogen الصديد اللي بيخرج هذا هو من الجن طلع + +46 +00:03:42,240 --> 00:03:47,660 +Portal of Exit واحد عنده Nasal Secretions يعني + +47 +00:03:47,660 --> 00:03:53,380 +إفرازات من الأنف هذه برضه عبارة عن Exit الـ Stool + +48 +00:03:53,380 --> 00:03:57,820 +الـ Urine عبارة عن Portal of Exit إذًا متفقين على + +49 +00:03:57,820 --> 00:04:01,740 +أن الـ Pathogen To Make an Infection It Should Have + +50 +00:04:01,740 --> 00:04:09,490 +an Exit طيب إذا خرج بده كمان طريقة انتقال وطرق + +51 +00:04:09,490 --> 00:04:14,070 +الانتقال عديدة ومتنوعة مش هنطرق لها دلوقت لكن بدنا + +52 +00:04:14,070 --> 00:04:20,130 +نتخيل إن ميكروب خرج إلى البيئة للماء مثلًا ولم + +53 +00:04:20,130 --> 00:04:24,810 +يشربه أحد يبقى إنكسرت الـ Cycle ودلوقت ما صار + +54 +00:04:24,810 --> 00:04:28,610 +Infection بعد فترة هيموت الـ .. الـ .. الـ .. الـ + +55 +00:04:28,610 --> 00:04:33,610 +Pathogen تمام؟ إذًا لازم يكون له طريق انتقال في بعض + +56 +00:04:33,610 --> 00:04:37,050 +طرق الانتقال على سبيل المثال قد لا تكون تقليدية + +57 +00:04:37,830 --> 00:04:45,730 +حشرة تأخذ عينة من دم مريض فتتشكل Exit بتشكل + +58 +00:04:45,730 --> 00:04:49,950 +للـ Pathogen Exit من مين؟ من المخزن المصاب صح؟ وقد + +59 +00:04:49,950 --> 00:04:55,030 +تكون أيضًا هي اللي تأخذ وجبة من إنسان سليم فتعمله + +60 +00:04:55,030 --> 00:05:00,190 +طريقة نقل طريقة نقل إذًا هناك طرق نقل متنوعة منها + +61 +00:05:00,190 --> 00:05:08,020 +الحشرة طيب طريقة النقل احنا كمان ممكن تكون عبر منافذ + +62 +00:05:08,020 --> 00:05:13,800 +مختلفة زي المخارج ما كانت مختلفة المداخل واخذوا + +63 +00:05:13,800 --> 00:05:18,220 +بالكم معظم الأحيان نفس المنافذ اللي هي المداخل هي + +64 +00:05:18,220 --> 00:05:24,760 +نفسها من المخارج الآن دخل الميكروب دخل وين؟ لازم + +65 +00:05:24,760 --> 00:05:28,680 +يدخل في عائل قابل للإصابة وبنسميها باللغة الإنجليزية + +66 +00:05:28,680 --> 00:05:33,970 +Susceptible Host يعني إيش إن عائل قابل للإصابة؟ قبل + +67 +00:05:33,970 --> 00:05:37,570 +كان في محاضرة تكلمنا عن Tissue و Species Tropicalism + +68 +00:05:37,570 --> 00:05:42,330 +أو الـ Tissue Tropicalism والـ Species Specificity لما + +69 +00:05:42,330 --> 00:05:46,690 +كنا إذا العائل ما لوش Receptor للبعض وجين يرتبط من + +70 +00:05:46,690 --> 00:05:49,830 +خلالها للبعض وجين ما تشايفها الـ Infection يبقى مش + +71 +00:05:49,830 --> 00:05:53,250 +كل ميكروب بدخل في أجسامنا بيعمل Infection لازم + +72 +00:05:53,250 --> 00:05:58,230 +نكون احنا Susceptible له قابلين للإصابة بهذا ال + +73 +00:05:58,230 --> 00:06:03,330 +Microbe إذا بدي Host قابل للإصابة طبعًا الـ Host القابل + +74 +00:06:03,330 --> 00:06:07,930 +للإصابة سوف يصاب و هيحصل تكاثر لمين؟ للـ Pathogen، + +75 +00:06:07,930 --> 00:06:10,910 +هيك اكتملت الـ Cycle اللي .. ليش بندرس هذا الـ Cycle + +76 +00:06:10,910 --> 00:06:15,890 +يعني؟ طب عشان أنا أجرب الموضوع أكثر بإنه نرجع + +77 +00:06:15,890 --> 00:06:18,650 +لدكتور عدنان الهندي في الـ Parasitology يعني دكتور عدنان + +78 +00:06:18,650 --> 00:06:22,410 +بيدرسنا و بيصر علينا إننا نحفظ الـ Life Cycle تبع الـ + +79 +00:06:22,410 --> 00:06:26,730 +Ascaris والـ Entamoeba والـ Protozoa وكل الديدان + +80 +00:06:26,730 --> 00:06:32,430 +لإن هو عارف أهمية الـ Life Cycle إن وين بنجوسها If + +81 +00:06:32,430 --> 00:06:35,330 +نمنع الـ Transmission طب تعالوا نطلع على الـ Cycle + +82 +00:06:35,330 --> 00:06:40,030 +لإن هذه مهمة الـ Cycle لو أنا بدي أمنع الـ Cycle of + +83 +00:06:40,030 --> 00:06:45,430 +Infection بدي أحارب المخازن مخزن، كل إنسان مصاب + +84 +00:06:45,430 --> 00:06:49,510 +بنتدور عليهم، إنسان مصاب بدنا نعالجه، إنسان Carrier + +85 +00:06:49,510 --> 00:06:53,550 +بدنا نعالجه، تمام؟ بدنا نعزله، مش ضروري نعالجه، + +86 +00:06:53,550 --> 00:06:57,030 +عزلناه، يعني في Actions متنوعة ومختلفة قد أتخذها + +87 +00:06:57,030 --> 00:07:00,450 +لمن اللي هو العدو، المخارج صعب التحكم فيها لكن + +88 +00:07:00,450 --> 00:07:05,140 +ممكن يكون في إرشادات، يا مريض ألبس كمامة يا مستقبل + +89 +00:07:05,140 --> 00:07:09,080 +ألبس كمامة تمام؟ يبقى فيها إنها إمكانية الأدوات + +90 +00:07:09,080 --> 00:07:12,600 +اللي استخدمناها وممكن نتعامل مع الـ Exits نعقمها يوم + +91 +00:07:12,600 --> 00:07:15,240 +إنت خلصت منها ده الـ Environment هاي كمان وسيلة من + +92 +00:07:15,240 --> 00:07:18,980 +الوسائل طرق النقل احنا جولناها حشرات مثلًا بنقول + +93 +00:07:18,980 --> 00:07:23,880 +طرق النقل إذا جولنا إن الحشرة X بتسبب أو بتنقل مرض + +94 +00:07:23,880 --> 00:07:29,300 +معين كفاح الحشرات بدل من كفاح من أدمين بنكافح من + +95 +00:07:29,300 --> 00:07:33,220 +الحشرات يبقى عندنا بطل على السلسلة + +96 +00:07:36,970 --> 00:07:41,350 +هو أكثر فعالية من الطرق وبنستخدمها لمنع اللي هو ال + +97 +00:07:41,350 --> 00:07:46,470 +Infection Cycle إذًا الـ Chain of Transmission أو + +98 +00:07:46,470 --> 00:07:50,250 +سلسلة الانتقال موضحة في كل أجزائها بالـ Pathogen بال + +99 +00:07:50,250 --> 00:07:55,130 +Reservoir بالـ Exit بالـ Transmission بالـ Portal of + +100 +00:07:55,130 --> 00:08:00,990 +Entry وبـ Susceptible Host عائل قابل أو Host قابل + +101 +00:08:00,990 --> 00:08:03,850 +للإصابة بالنسبة للـ Transmission اللي حاطينه هنا + +102 +00:08:03,850 --> 00:08:06,370 +مجموعة من الطرق زي مثلًا Airborne + +103 +00:08:11,870 --> 00:08:14,250 +المراتب المنتقلة عن طريق اللي هو الغذاء وعن طريق + +104 +00:08:14,250 --> 00:08:16,690 +الـ Water وأنا هحط نموذج احنا اللي هو Sexual + +105 +00:08:16,690 --> 00:08:24,910 +Contact Routes of Infection مداخل اللي هو أو من أين + +106 +00:08:24,910 --> 00:08:28,410 +تأتي أو من أين تدخل الميكروبات عبر الـ Respiratory + +107 +00:08:28,410 --> 00:08:31,370 +Tract وأنا بتقول لكم المرة أذكركم إن الـ Portals of + +108 +00:08:31,370 --> 00:08:34,550 +Entry هم تقريبًا نفسهم الـ Portal of Exit + +109 +00:08:37,250 --> 00:08:41,910 +عطس، كحة، كل هذه ممكن تنقل إيه؟ Troubles وتخيلوا + +110 +00:08:42,820 --> 00:08:47,320 +العطسة الواحدة بتطلع ملايين .. ملايين الـ Particles + +111 +00:08:47,320 --> 00:08:52,080 +تمام؟ جوا كل Particle قد تحمل فيروس أو بكتيريا + +112 +00:08:52,080 --> 00:08:56,220 +تمام؟ وبتنشرها وقد تصل المسافة القذيفة اللي + +113 +00:08:56,220 --> 00:08:59,320 +خارجة من الأنف إلى مسافة متر ونصف عشانك احنا + +114 +00:08:59,320 --> 00:09:02,500 +بنقول على الأقل اللي بدك بينك وبين الـ .. الآخر + +115 +00:09:02,500 --> 00:09:05,760 +يعني في .. لما إنها أكبر إنك مسافة أكثر من متر + +116 +00:09:05,760 --> 00:09:09,220 +إيه؟ ونصف عتابة من مصطلح القذيفة يعني احنا .. + +117 +00:09:09,220 --> 00:09:11,520 +احنا بناخد مصطلحاتنا من بيئتنا يعني من أين نجيبها + +118 +00:09:13,890 --> 00:09:17,370 +بتقلل نعم بتقلل من المسافة لكن هذا لا يمنع + +119 +00:09:17,370 --> 00:09:21,950 +الانتشار لإنه حتى الإيدين مش Completely Closed + +120 +00:09:21,950 --> 00:09:24,490 +يعني المناطق وهو هيخرج من هنا ومن هنا كمان لكن + +121 +00:09:24,490 --> 00:09:28,870 +بيقلل الكمية Gastrointestinal + +122 +00:09:28,870 --> 00:09:32,150 +عبر اللي هو الـ Gastrointestinal Tract Water or + +123 +00:09:32,150 --> 00:09:35,830 +Food Genitourinary Tract اللي هي القناة البولية + +124 +00:09:35,830 --> 00:09:40,450 +الجنسية Close Contact الاتصال المباشر Insect Bite + +125 +00:09:40,450 --> 00:09:48,600 +عضّات الحشرات الحشرات غالبًا بتستخدمنا لوجبة دم زي + +126 +00:09:48,600 --> 00:09:54,460 +البعوض أو الحشاطات زي الـ bugs أو الفليز bugs اللي + +127 +00:09:54,460 --> 00:09:59,480 +هي الـ بق بلغة عربية blood transfusion وده إيه + +128 +00:09:59,480 --> 00:10:02,300 +طبعا يعني دخيلة على الطب ما كانش مولود على طريقة + +129 +00:10:02,300 --> 00:10:06,180 +كان زمان يعني جاب المئة عامة كانت حد اسمها blood + +130 +00:10:06,180 --> 00:10:08,700 +transfusion لكن الآن صارت جزء من الـ medical + +131 +00:10:08,700 --> 00:10:15,360 +practicesخدوا بالكوا كتير من الإجراءات الطبية قد + +132 +00:10:15,360 --> 00:10:20,440 +تسبب لنا infection وهذه واحدة من النقاط اللي .. + +133 +00:10:20,440 --> 00:10:24,460 +لما إحنا بنقول hospital acquired infection جداش + +134 +00:10:24,460 --> 00:10:27,980 +الإنسان بيتعرض لإجراءات طبية، كام قبر بياخد في + +135 +00:10:27,980 --> 00:10:32,380 +المستشفى، كام canula، كام جراحة، جداش بزيد فرص إن + +136 +00:10:32,380 --> 00:10:37,480 +يصيب بـinfectionالـ parental roots وعن طريق الحقن + +137 +00:10:37,480 --> 00:10:40,780 +الـ mucous membranes الأشياء المخاطية وأنا مرة + +138 +00:10:40,780 --> 00:10:44,160 +ذكرتلكوا الـ finger to eye فاكرينها؟ finger to eye + +139 +00:10:44,160 --> 00:10:46,980 +أحد الأشياء المهمة جدا في موضوع اللي هو الـ + +140 +00:10:46,980 --> 00:10:52,530 +transmissionطيب we are going to classify infection + +141 +00:10:52,530 --> 00:10:57,050 +according to infectious state الحالة اللي بيمر + +142 +00:10:57,050 --> 00:11:01,650 +فيها المريض نفسه بتساعدنا في تصنيف أنواع من العدوى + +143 +00:11:01,650 --> 00:11:05,310 +مثلا بقولنا في نوع من العدوى اسمه in apparent و in + +144 +00:11:05,310 --> 00:11:10,050 +apparent عكس apparent apparent معناها ظاهر واضح + +145 +00:11:10,050 --> 00:11:15,890 +أمام بينما in apparent غير واضح إلا اسم تاني أشهر + +146 +00:11:15,890 --> 00:11:19,970 +منها اسمه subclinical infection subclinical يعني + +147 +00:11:19,970 --> 00:11:25,570 +صار في عدوى وصار فيه تكاثر للبكتيريا لكن لم يظهر + +148 +00:11:25,570 --> 00:11:30,750 +هناك أي أعراض عشان تسميها subclinical infection + +149 +00:11:30,750 --> 00:11:35,730 +رقم اتنين latent infection وهذا مختلف تماما عن الـ + +150 +00:11:35,730 --> 00:11:39,330 +inapparent أو الـ subclinical خدوا مثال على الـ + +151 +00:11:39,330 --> 00:11:43,890 +latent infection عشان بحلزمنا كمان شوية بعض البعض + +152 +00:11:43,890 --> 00:11:47,730 +مننا وأنا منهم أصاب بحمل بعد + +153 +00:11:50,130 --> 00:11:53,930 +هذا الحم هو مش حم بمفهوم إنه من حرارة الجسم هذا + +154 +00:11:53,930 --> 00:11:57,290 +عبارة عن viral infection اسمها herpes simplex type + +155 +00:11:57,290 --> 00:12:00,890 +1 الآن + +156 +00:12:00,890 --> 00:12:07,130 +مش موجود لكن هو موجود موجود ومُقتبل بينتظر فرصة + +157 +00:12:07,130 --> 00:12:13,570 +تهيئة الظروف المناسبة له مثلا أصاب بـ influenza مرة + +158 +00:12:13,570 --> 00:12:19,400 +أخرى أنا في الجسم أنا اعتباره بضعف بسبب الـ dryness + +159 +00:12:19,400 --> 00:12:23,280 +بعض الأحيان جفاف فيه حاجة باسمها inciting factors + +160 +00:12:23,280 --> 00:12:29,400 +عوامل بتحفظه على الخروج لكن كلها مرتبطة بأن + +161 +00:12:29,400 --> 00:12:34,400 +المناعة تكون منخفضة عند الشخص الأقل عندي ما عنديش + +162 +00:12:34,400 --> 00:12:39,840 +لكن موجود أو مش موجود هو موجود هذا بيموت معانا يعني + +163 +00:12:39,840 --> 00:12:44,080 +يبتسم مرة واحدة على سبيل المثال تمام؟ ويبقى الـ + +164 +00:12:44,080 --> 00:12:46,980 +virus اللي بحكي عنه اللي هو الـ Herpes Simplex في + +165 +00:12:46,980 --> 00:12:52,480 +virus تاني بتسمعوا فيه Varicella Zoster Varicella + +166 +00:12:52,480 --> 00:12:55,920 +Zoster Varicella اللي هي الـ chicken box جدر الميه + +167 +00:12:55,920 --> 00:12:59,160 +اللي أنتو تعرفوه جدر الميه اسمه بالإنجليزية + +168 +00:12:59,160 --> 00:13:04,100 +chicken box بينصب فيه غالبا إحنا الصغار بعد تلاتين + +169 +00:13:04,100 --> 00:13:08,590 +سنة أربعين سنة خلاص طبعا إحنا بنطيب منه بعد ترديس + +170 +00:13:08,590 --> 00:13:13,710 +الأربعين سنة بنلاجأ طلعنا حب أو blisters على .. في + +171 +00:13:13,710 --> 00:13:17,890 +الظهر، بيسموها الحزام الناري، تسمعوا فيه، الحزام + +172 +00:13:17,890 --> 00:13:23,490 +الناريهذا الحزام الناري هي عبارة عن activation أو + +173 +00:13:23,490 --> 00:13:27,130 +reactivation لـ latent infection بالفارسيلا دخل + +174 +00:13:27,130 --> 00:13:34,150 +فارسيلا وطلع زوستا بعد كام سنة يعني عمل latency + +175 +00:13:34,150 --> 00:13:38,350 +period لكن النقطة الحلوة في الموضوع هنا اللي بدنا + +176 +00:13:38,350 --> 00:13:43,950 +نفهمها إنه غير معدي لما بيكون latent لما بيكون + +177 +00:13:43,950 --> 00:13:48,480 +latent بيكون غير معدي كامن ساكن ما بيتكاتر بتالي + +178 +00:13:48,480 --> 00:13:52,200 +غير معدى ليش ذكرت المعلومة دي عشان أنا .. عشان + +179 +00:13:52,200 --> 00:13:55,620 +أميزها عن رقم أربعة رقم أربعة اللي هي الـ career + +180 +00:13:55,620 --> 00:14:04,480 +state حامل للمرض حامل وبينشر الـ micro وبينشر الـ + +181 +00:14:04,480 --> 00:14:10,040 +micro بينما الأولارش كان كامن ومش معدى أحكيكوا قصة + +182 +00:14:10,040 --> 00:14:15,890 +عن الـ career state في واحدة اسمها Mary اشتهرت + +183 +00:14:15,890 --> 00:14:19,890 +تاريخيا في الطب باسم tayfoy and married، ما أسوأ + +184 +00:14:19,890 --> 00:14:24,310 +إن الإنسان يتلقى بـ tayfoy، بسلمونيللا تايفي، تمام؟ + +185 +00:14:25,200 --> 00:14:28,360 +هذه قصة مشهورة زي اللي عملت جوجلينج عليها هتشوفوها + +186 +00:14:28,360 --> 00:14:32,440 +على الإنترنت بشكل يعني كتير مبتوب عنها هذه سيدة + +187 +00:14:32,440 --> 00:14:37,160 +فقيرة كانت تعمل في بريطانيا وكانت حامل لميكروب + +188 +00:14:37,160 --> 00:14:42,720 +السلمونيلاتايفين حامل بالميكروب وليست لا يقضي + +189 +00:14:42,720 --> 00:14:45,520 +عليها أي أعراض والدنيا لو عندها أعراض كانت مشتغلة + +190 +00:14:45,520 --> 00:14:50,620 +كانت بصحة جيدة وكانت تعمل في أعمال وضيع منها الطهي + +191 +00:14:50,620 --> 00:14:57,480 +والتنظيف في مؤسسات يعني أينما حلت ماري حلت tayfoyd + +192 +00:14:57,480 --> 00:15:01,000 +ده مؤشر على مستوى النظافة عندها كمان سيء مش بس + +193 +00:15:01,000 --> 00:15:03,620 +فقيرة بتشتغل في أعمال وضيعها كمان مستوى النظافة + +194 +00:15:03,620 --> 00:15:08,940 +عندها سيء لإنه من الـ stool تبعها بينتقل للناس لإنه + +195 +00:15:08,940 --> 00:15:11,580 +استعمال الدايفي بيصير الشدن في وين؟ في الـ stool + +196 +00:15:11,580 --> 00:15:16,660 +المهم في كان طبيب بيتبع الوباء تبع tayfoyd في + +197 +00:15:16,660 --> 00:15:20,140 +بريطانيا وكان كل ما يصير في منطقة يروح يدرس + +198 +00:15:20,140 --> 00:15:20,660 +المنطقة + +199 +00:15:24,340 --> 00:15:29,960 +وصار فيها الوباء في سجن لجأ إنه ماري تشتغل طبخة في + +200 +00:15:29,960 --> 00:15:35,940 +السجن طبعا فهرفه إنه الـ .. الـ .. الـ .. طبعا هي زي + +201 +00:15:35,940 --> 00:15:39,840 +ما قلتلكوا هي هذه الـ career estate نموذج رائعة على + +202 +00:15:39,840 --> 00:15:43,200 +الـ career estate إنه هي لا تعاني من أي أعراض فكان + +203 +00:15:43,200 --> 00:15:46,340 +الصبح في سؤال عند الطلاب إنه من الذي يجعل الـ + +204 +00:15:46,340 --> 00:15:51,650 +microbe يعني يتخذ هذا الشكل من الـ .. من الأشكالهو + +205 +00:15:51,650 --> 00:15:54,630 +غالبا بيكون عمل infection وعمل .. ممكن يكون عمل + +206 +00:15:54,630 --> 00:15:58,150 +disease كمان يا بنات وطابت منه .. أو طابت منه + +207 +00:15:58,150 --> 00:16:02,390 +الشخص يعني .. شوف يا منه الشخص لكن وصل لمكان لا هو + +208 +00:16:02,390 --> 00:16:07,390 +جادر يتعامل بكميات كبيرة كاتر ولا هو .. ولا جهاز + +209 +00:16:07,390 --> 00:16:09,730 +الملاعق جادر يتخلص منه زي مثلا ما بيصير في الـ + +210 +00:16:09,730 --> 00:16:12,670 +gallbladder بيصير الميكروبلم إيه للجوال؟ blood + +211 +00:16:13,820 --> 00:16:17,380 +الـ gallbladder تفرز bile salts، الـ bile salts معروف عنها + +212 +00:16:17,380 --> 00:16:20,900 +أنها antimicrobial activity عالية، ثانوية اللي + +213 +00:16:20,900 --> 00:16:24,020 +مقاومة، بس مش مقاومة hundred percent، بمعنى أنها + +214 +00:16:24,020 --> 00:16:28,780 +بتموت، فبالتكاثر بيكون ضئيل، مش جادر تصل الـ + +215 +00:16:28,780 --> 00:16:31,780 +numbers يعني يعمل disease، quantum sensing، + +216 +00:16:31,780 --> 00:16:35,360 +فاكرين؟ ولا جادر الجسم يتخلص منها لأنها موجودة في + +217 +00:16:35,360 --> 00:16:39,400 +مكان هي resistant نوعا ما كيلو أو أنها ممكن تتخذ + +218 +00:16:39,400 --> 00:16:43,890 +شكل normal microbiota في هذا الإنسان إحنا مش كنا الـ + +219 +00:16:43,890 --> 00:16:46,770 +microbiota بيصير فيها نوع من اتزان، فهي الورقة + +220 +00:16:46,770 --> 00:16:49,530 +اللي أنا صارت متزينة مع مين، والسؤولي مكان أنا + +221 +00:16:49,530 --> 00:16:52,470 +بتعيش بينكوا كـ normal microbiota، مافي إمكانية أن + +222 +00:16:52,470 --> 00:16:56,010 +تصل للـ number العادل تبعها، إذن ميزنا بالـ latent + +223 +00:16:56,010 --> 00:17:01,110 +infection وبين الـ carrier stigma رقم واحد ورقم + +224 +00:17:01,110 --> 00:17:05,110 +تلاتة عكس بعض تماما الأولانية inapparent التانية + +225 +00:17:05,110 --> 00:17:07,530 +apparent اللي هي الـ disease اللي إحنا بنشهر فيه + +226 +00:17:07,530 --> 00:17:12,670 +وبيظهر هنا أعراض واضحة لهذا المرض إذا inapparent + +227 +00:17:12,670 --> 00:17:16,310 +Latin ما حدش يقلّي بعدها كده إيش الفروقات بينهم + +228 +00:17:16,310 --> 00:17:19,230 +واضحين لإنه الفروقات بينهم لإنه apparent إنه أصلا + +229 +00:17:19,230 --> 00:17:23,470 +ما عرفناها إحنا إحنا ما شوفناها كيف عرفنا إنه مصاب؟ + +230 +00:17:23,470 --> 00:17:27,330 +ممكن نعمل فحص رلوجي نعرف إن في عنده antibodies لكن + +231 +00:17:27,330 --> 00:17:29,950 +هو لم يشهر ولم يظهر عليه + +232 +00:17:35,650 --> 00:17:40,210 +بنشوف الحم فيها بيشفق، بيختبئ الـ .. الـ microbe + +233 +00:17:40,210 --> 00:17:43,770 +لفترة معينة، إذا توفرته الظروف بيرجع مرة تانية، + +234 +00:17:43,770 --> 00:17:48,790 +لكن خلال فترة اختفاقه هو ليس مؤدي، الـ apparent + +235 +00:17:48,790 --> 00:17:53,270 +infection كل الصفات موجودة فيها الـ carrier state + +236 +00:17:53,270 --> 00:17:57,610 +هي صفة حامل للميكروب ولكن بيكون برضه أو عنده + +237 +00:17:57,610 --> 00:18:01,350 +القدرة إنه يعدي الآخر بس تحس يعني قصة الحم هي ممكن + +238 +00:18:01,350 --> 00:18:07,880 +تيجي من شخص لآخر؟ عشان هيك اللي عنده ممنوع يجرب + +239 +00:18:07,880 --> 00:18:11,140 +الأطفال يعملوا kissing أو يلعبهم يعني يقبل الأطفال + +240 +00:18:11,140 --> 00:18:15,620 +تقبيل الأطفال من الأشياء اللي بتنقل الـ micro .. الـ + +241 +00:18:15,620 --> 00:18:18,900 +.. الـ .. الـ herpes بيسموها kissing disease لإنه + +242 +00:18:18,900 --> 00:18:21,740 +بينتقل عن طريق التقبيل وبالتالي الإنسان اللي عنده + +243 +00:18:21,740 --> 00:18:29,720 +حم يبتعد قدر الإمكان عن إيه؟ عن الأطفال طيب هذا + +244 +00:18:29,720 --> 00:18:32,700 +كان تصنيف infection state تصنيف على حسب الـ + +245 +00:18:32,700 --> 00:18:33,820 +infection site + +246 +00:18:37,560 --> 00:18:43,120 +إذا كان في مكان محدود محدود جغرافيا في الإنسان مثل + +247 +00:18:43,120 --> 00:18:48,200 +حدبة دم التمام تيتانوس في الـ tonsils هذا بسميه + +248 +00:18:48,200 --> 00:18:52,020 +localized أو local infection عكسه systemic أو + +249 +00:18:52,020 --> 00:18:55,900 +generalized والـ generalized بكون ماخد system مثلا + +250 +00:18:55,900 --> 00:18:59,820 +الـ lungs أو الـ respiratory system الـ gastro + +251 +00:18:59,820 --> 00:19:03,320 +intestinal system ممكن يكون في الدم لكن طلعوا + +252 +00:19:06,280 --> 00:19:11,040 +متشابهة مع بعض كيف نميزها مع بعض خذوا بالكم لو + +253 +00:19:11,040 --> 00:19:15,780 +أنهم أو لو في منهم اثنين من التعريفات متطابقين كان + +254 +00:19:15,780 --> 00:19:21,300 +معطينهم تعريفين بس للأسف نحن عدم قدرتنا على + +255 +00:19:21,300 --> 00:19:23,940 +التمييز بين الـ terms بتخلينا نستخدمهم + +256 +00:19:23,940 --> 00:19:28,300 +interchangeably يعني أعطيكم مثال ثلاثة وأربعة + +257 +00:19:28,300 --> 00:19:34,620 +bacteremia and septicemia رأيتموه؟ لو رحنا لطبيب من + +258 +00:19:34,620 --> 00:19:39,160 +الذي يعالجه المرضى وعمل الـ blood culture عند شخص + +259 +00:19:39,160 --> 00:19:41,400 +معين وطلع عنده بكتيريا، ممكن الطبيب يقول عنده + +260 +00:19:41,400 --> 00:19:46,400 +بكتيريميا وآخر يقول عنده A والاثنان يفهموا بعض، + +261 +00:19:46,400 --> 00:19:49,140 +الاثنان يفهموا بعض أن واحد يتكلم عن بكتيريا في + +262 +00:19:49,140 --> 00:19:52,860 +الدم وواحد عن بكتيريا في الدم، لكن دائماً وأبداً + +263 +00:19:52,860 --> 00:19:57,200 +دقة المصطلح هي التي خلتنا أن نعمل مصطلح ثاني + +264 +00:19:58,070 --> 00:20:00,610 +فبالتالي ليش نحن .. إذا كان هما اثنان واحد لأ + +265 +00:20:00,610 --> 00:20:04,030 +نلغي واحد نسخ دم؟ و .. لأ .. واقعي أنا هما اثنان + +266 +00:20:04,030 --> 00:20:07,470 +مختلفان عن بعضهما البعض نبدأ من الأول toxemia + +267 +00:20:07,470 --> 00:20:13,050 +تعبيراً عن وجود الـ exotoxin في الدم endotoxemia + +268 +00:20:13,050 --> 00:20:16,730 +تعبيراً عن وجود ميه؟ الـ endotoxin في الدم سهل هذا + +269 +00:20:16,730 --> 00:20:21,510 +the bacterium is an invasion of the bloodstream of + +270 +00:20:21,510 --> 00:20:27,310 +a bio bacteria يعني البكتيريا وصلت للدم لمجرد دمما + +271 +00:20:27,310 --> 00:20:30,670 +تحدثنا عن تفاصيل، قلنا البكتيريا مردودة الآن إيه؟ + +272 +00:20:30,670 --> 00:20:34,430 +أعطيكم مثال، واحد يفرج أسنانه، بتعرفوا فيه بعض + +273 +00:20:34,430 --> 00:20:38,910 +الأحيان لأننا مشاكل في اللثة، ممكن الدم ينزل، الفم + +274 +00:20:38,910 --> 00:20:42,830 +مليء بالبكتيريا، عشرة أوص، تسعة، لكل واحد ملي من ال + +275 +00:20:42,830 --> 00:20:47,810 +saliva، أرقام صعبة نعدها، إمكانية أنه تدخل + +276 +00:20:47,810 --> 00:20:51,250 +البكتيريا من مكان الجرح الذي جرح هنا من نظر في + +277 +00:20:51,250 --> 00:20:55,830 +أسناننا، موجودة، لو أنا أخذت blood culture مصادفة + +278 +00:20:56,380 --> 00:20:59,480 +ممكن أعزل البكتيريا التي طلعت من هنا هذه بكتيريا + +279 +00:20:59,480 --> 00:21:03,340 +وسموها transient bacteria transient إيش يعني + +280 +00:21:03,340 --> 00:21:08,360 +transient يعني عابرة لأن عددها قليل وغير مباطلين + +281 +00:21:08,360 --> 00:21:13,320 +الجسم يتخلص منها يبقى ده mere presence إيش معنى + +282 +00:21:13,320 --> 00:21:20,420 +مجرد وجود البكتيريا في الدم نسميه بكتيريا أما + +283 +00:21:20,420 --> 00:21:27,230 +الـ septicemia فيها مصطلح bacteremia زائد toxemia معاً + +284 +00:21:27,230 --> 00:21:33,390 +يعني البكتيريا مش بس وصلت الدم بل تكاثرت في الدم + +285 +00:21:33,390 --> 00:21:37,490 +ووصل عددها إلى الـ quorum sensing وبدأت تنتج إيش؟ + +286 +00:21:37,490 --> 00:21:41,330 +الـ toxemia إذا septicemia معناها أن البكتيريا مولدة + +287 +00:21:41,330 --> 00:21:46,370 +في الدم بالإضافة إلى الـ toxemia الـ pyemia هي + +288 +00:21:46,370 --> 00:21:50,410 +infection of the blood by a biogenic + +289 +00:21:54,030 --> 00:21:57,490 +Biogenic Infection يسموها باس بيوزن باس بيوزن باس + +290 +00:21:57,490 --> 00:22:02,850 +بيوزن + +291 +00:22:02,850 --> 00:22:05,650 +باس بيوزن باس بيوزن باس بيوزن باس بيوزن باس بيوزن + +292 +00:22:05,650 --> 00:22:05,830 +باس بيوزن باس بيوزن باس بيوزن باس بيوزن باس بيوزن + +293 +00:22:05,830 --> 00:22:05,930 +باس بيوزن باس بيوزن باس بيوزن باس بيوزن باس بيوزن + +294 +00:22:05,930 --> 00:22:07,130 +باس بيوزن باس بيوزن باس بيوزن باس بيوزن باس بيوزن + +295 +00:22:07,130 --> 00:22:09,110 +باس بيوزن باس بيوزن باس بيوزن باس بيوزن باس بيوزن + +296 +00:22:09,110 --> 00:22:14,590 +باس بيوزن باس بيوزن باسالي هي الـ streptococcus + +297 +00:22:14,590 --> 00:22:18,010 +biodeins اسمها streptococcus biodeins التي تسبب + +298 +00:22:18,010 --> 00:22:21,410 +تنسولايتس عشان كده قلت من حبكم لأنها جزء من + +299 +00:22:21,410 --> 00:22:26,770 +حياتنا الطفولية أنه كثير مرات كده التي قصبنا بهذا + +300 +00:22:26,770 --> 00:22:27,530 +الـ micro + +301 +00:22:39,060 --> 00:22:42,180 +هتبنيها مرحلة أن ده البكتيريا داخلة الدم تيجي ده + +302 +00:22:42,180 --> 00:22:46,540 +مخيار من خارج أما أن تنجح في التكاثر وتصل + +303 +00:22:46,540 --> 00:22:51,640 +لستسيميا هذا خيار أو أن هي أن يجب أن تبقى لوتالي + +304 +00:22:51,640 --> 00:22:57,800 +وتبطل لستسيميا إذا في إتلاف بينهم طيب + +305 +00:22:57,800 --> 00:23:01,700 +شايفين الشيء على اللوحة أنتم أنا مش شايف على + +306 +00:23:01,700 --> 00:23:05,780 +اللوحة أي شيء بس لا بس أن تطلع على الـ .. الـ .. الـ + +307 +00:23:11,560 --> 00:23:15,040 +في عندي لو حالة بتقول gram أو some organisms of + +308 +00:23:15,040 --> 00:23:19,300 +medical importance خذوا بالكم أنا أكثر من مرة في + +309 +00:23:19,300 --> 00:23:23,280 +أكثر محاضرة حطيت جداول وفيها أسماء بكتيريا وقرأنا + +310 +00:23:23,280 --> 00:23:27,880 +.. وقرأنا .. وقرأنا أسماء البكتيريا والقراءة هنا + +311 +00:23:27,880 --> 00:23:31,780 +مرة بتكون .. أول مرة بتكون to be familiarized يعني + +312 +00:23:31,780 --> 00:23:35,460 +عشان نخليكم familiar أن الاسم يبطل غريب بس بعد ما + +313 +00:23:35,460 --> 00:23:39,600 +بطل غريب الأصل نحن يعني بالفعل نعرف عنه بعض + +314 +00:23:39,600 --> 00:23:40,780 +المعلومات البسيطة + +315 +00:23:45,480 --> 00:23:51,360 +هنا لـ gram negative cocci زي مين النيسيريا أعتقد في + +316 +00:23:51,360 --> 00:23:55,020 +اسمين في النيسيريا المفروض أن هما صاروا معروفين لكم + +317 +00:23:55,020 --> 00:24:00,780 +Neisseria gonorrhoeae و Neisseria meningitidis أو + +318 +00:24:00,780 --> 00:24:05,760 +meningitis في حرف الـ D خفيف طبعاً الـ meningitis هي + +319 +00:24:05,760 --> 00:24:11,130 +المرض بس الـ bacterium meningitidis إذن نيسيريا كمثل + +320 +00:24:11,130 --> 00:24:15,250 +أساسي عن مجموعة بخمة .. مجموعة بخمة .. مجموعة + +321 +00:24:15,250 --> 00:24:19,750 +صغيرة من الـ gram negative cocci أو يسموها gram + +322 +00:24:19,750 --> 00:24:24,870 +negative diplococci على + +323 +00:24:24,870 --> 00:24:28,430 +الجهة الثانية الـ gram positive cocci facultative + +324 +00:24:28,430 --> 00:24:32,210 +anaerobic الـ facultative anaerobic ممثل عنهم + +325 +00:24:32,210 --> 00:24:36,290 +الاستفيلو cocci و الاستريبتو cocci أو استريبتو + +326 +00:24:36,290 --> 00:24:40,610 +كوكس واستفيلو كوكس طيب الآن في الستافيلو كوكاس أكثر + +327 +00:24:40,610 --> 00:24:49,670 +اسم بتعرفه؟ خلينا نسمع من الذي قاعدين وراء هناك وإيش + +328 +00:24:49,670 --> 00:24:55,830 +كمان؟ بس أو yes؟ + +329 +00:25:01,420 --> 00:25:04,700 +أسوأ .. أسوأ حاجة في مرحلة الحفظ أنه يصير نأخذة من + +330 +00:25:04,700 --> 00:25:08,240 +.. اسم من هنا ونضعه على اسم الثاني من هنا، فواضح + +331 +00:25:08,240 --> 00:25:10,420 +أنه فيه يركب أسماء جديدة من هنا ونضعه على + +332 +00:25:10,420 --> 00:25:13,140 +الشجرة الثانية، الـ pneumonia is streptococcus + +333 +00:25:13,140 --> 00:25:15,780 +pneumonia، مش Staphylococcus pneumonia، الـ + +334 +00:25:15,780 --> 00:25:19,120 +Staphylococcus epidermis، epidermis على سبيل المثال + +335 +00:25:19,120 --> 00:25:23,840 +مشهور جداً في Staphylococcin في الـ streptococcus + +336 +00:25:23,840 --> 00:25:26,140 +نحن لسه ذكرنا أحد قبل قليل، streptococcus + +337 +00:25:26,140 --> 00:25:29,360 +pyogenes، و streptococcus pneumonia، و + +338 +00:25:29,360 --> 00:25:31,980 +streptococcus الـ pneumonia، وفي streptococcus + +339 +00:25:31,980 --> 00:25:36,880 +agalactia، أسماء مشهورة في الـ streptococcus ثلاثة + +340 +00:25:36,880 --> 00:25:38,120 +الذي ذكرناهم الـ pneumonia + +341 +00:25:41,870 --> 00:25:45,770 +آخرهم اسمهم المشهورين Aureus التي هي الذهبية و + +342 +00:25:45,770 --> 00:25:49,850 +Epidermis واضح من اسمها أنها موجودة على الجلد + +343 +00:25:49,850 --> 00:25:56,230 +نأتي للـ Spirochetes زي تريبونيميا ودرسنا بكتيريا + +344 +00:25:56,230 --> 00:26:00,030 +واحدة منهم على الأقل تريبونيماباليدوم التي تسبب + +345 +00:26:00,030 --> 00:26:05,470 +مرض السّفلس و الـ Borrelia Borrelia أيضاً من ال + +346 +00:26:05,470 --> 00:26:08,830 +spiral microorganism أخذنا اسم Borrelia + +347 +00:26:08,830 --> 00:26:14,420 +burgdorferi اسمه صعب شوية بوريليا بيرجدرفيري و + +348 +00:26:14,420 --> 00:26:19,660 +تسبب مرضاً بقى اسمه Lyme disease Lyme disease + +349 +00:26:19,660 --> 00:26:28,280 +Leptospira Leptospira من اسمها أيضاً أشبه بوريليا و + +350 +00:26:28,280 --> 00:26:34,100 +Leptospira it's a spiral microorganism كان جنبهم + +351 +00:26:34,100 --> 00:26:39,400 +Peptococcus + +352 +00:26:39,400 --> 00:26:39,860 +و Peptospira + +353 +00:26:43,380 --> 00:26:46,060 +وكويس الاسمانة بأول مرة عشان يكون ثاني مرة يكون + +354 +00:26:46,060 --> 00:26:50,920 +اسمهم مش أول مرة بيبتوكوكس و بيبتوستريبتوكوكس + +355 +00:26:50,920 --> 00:26:55,460 +هدول الاثنين نسميهم anaerobic cocci anaerobic + +356 +00:26:55,460 --> 00:26:59,880 +cocci يعني من ميزاتهم أنهم يشبهوا الستريبتوكوكس + +357 +00:26:59,880 --> 00:27:07,860 +لكن لا هوائية إشباعية spiral gram negative زي + +358 +00:27:07,860 --> 00:27:10,720 +نتالاين مشهورين جداً الـ campylobacter + +359 +00:27:13,410 --> 00:27:17,050 +أنا أقول لكم عرفتوها إيه بسبب الهيليكوباكتر؟ و نريد + +360 +00:27:17,050 --> 00:27:22,310 +إجابة من شخص واحد الهيليكوباكتر + +361 +00:27:22,310 --> 00:27:27,110 +بيادة + +362 +00:27:27,110 --> 00:27:32,070 +الخسارة القرح + +363 +00:27:32,070 --> 00:27:36,890 +الهيليكوباكتر causes peptic ulcer تمام؟ و كنا تسعة + +364 +00:27:36,890 --> 00:27:40,790 +وتسعين تسعة وعشرة من القرح المعدية هي caused by + +365 +00:27:44,680 --> 00:27:48,260 +الـ can be lobacter خذوا المعلومة عنها عشان + +366 +00:27:48,260 --> 00:27:52,760 +ماننسهاش it is number one cause number one cause + +367 +00:27:52,760 --> 00:27:58,600 +of diarrhea in the middle east among children وصلت + +368 +00:27:58,600 --> 00:28:03,640 +المعنى ده لسه هي المسبب رقم واحد للإسهال في منطقة + +369 +00:28:03,640 --> 00:28:07,040 +الشرق الأوسط لدى الأطفال وهي من الميكروبات + +370 +00:28:07,040 --> 00:28:11,530 +المهملة فيهالقطارات إيش يعني مهملة؟ لأ يعني + +371 +00:28:11,530 --> 00:28:15,410 +لا يُدَرَس عليها في حالات إسهالية مع أنها أصبحت .. + +372 +00:28:15,410 --> 00:28:18,870 +أصبحت يزوء من الـ routine في الـ school culture في + +373 +00:28:18,870 --> 00:28:25,630 +بعض البلدان السيد ما يعرفش الاسم؟ كامبيلو باكتر أو + +374 +00:28:25,630 --> 00:28:32,650 +كامبيلوباكتر طيب + +375 +00:28:32,650 --> 00:28:36,110 +بالنسبة للـ Endospore forming groundpost في بسراي + +376 +00:28:36,110 --> 00:28:37,890 +فيها أسماء + +377 +00:28:42,820 --> 00:28:48,320 +أيّ الثلاث في أسماء من الـ Baselas؟ Antracis تمام، + +378 +00:28:48,320 --> 00:28:55,220 +كويس، تمام، هاي .. هاي .. هاي تلاتة وواحد، يعني + +379 +00:28:55,220 --> 00:29:00,180 +هناك الآن عرفنا الأربع أسماء تحت عنوان Gram-Positive + +380 +00:29:00,180 --> 00:29:05,440 +-Endospore-Forming Bacilli طيب فينا Gram negative + +381 +00:29:05,440 --> 00:29:11,420 +aerobic bacilli زي الجميلة Pseudomonas الجنوزة + +382 +00:29:11,420 --> 00:29:17,640 +ليست منها الجميلة لأنها تنتج صبغة زرقاء صفراء + +383 +00:29:17,640 --> 00:29:24,130 +خضراء وما بينهما تمام يعني ممكن تكون ألوان كمان + +384 +00:29:24,130 --> 00:29:26,950 +شغل ثاني سميناها الجميلة عشان .. عشان هي إلها رائحة + +385 +00:29:26,950 --> 00:29:31,370 +ذكية، يعني بتنتج fruity .. fruity odor، بسموها .. + +386 +00:29:31,370 --> 00:29:34,690 +إيش يعني fruity؟ يعني فاكهة، عليها الفاكهة، أول ما + +387 +00:29:34,690 --> 00:29:38,490 +نفتح .. نفتح الطبق في ال .. في المختبر، نشتم رائحة + +388 +00:29:38,490 --> 00:29:42,630 +جميلة جدا من الـ Pseudomonas، فعلا شكلا ورائحة هي + +389 +00:29:42,630 --> 00:29:47,290 +جميلة، لكن .. من أسوأ أنواع الـ infection في + +390 +00:29:47,290 --> 00:29:51,490 +المستشفيات لأنها it causes hard to treat infection + +391 +00:29:51,490 --> 00:29:54,810 +it is antimicrobial resistant أو has high + +392 +00:29:54,810 --> 00:29:59,790 +antimicrobial resistance الميكروبيل + +393 +00:29:59,790 --> 00:30:02,650 +التانية في نفس المجموعة الـ Bordetella و الـ + +394 +00:30:02,650 --> 00:30:06,970 +Bordetella سمعتوا فيها باسم Bordetella Pertussis + +395 +00:30:06,970 --> 00:30:12,430 +اللي درسناها في الـ vaccine اللي اسمه DPT طيب مين + +396 +00:30:12,430 --> 00:30:12,830 +مش ذاكر + +397 +00:30:17,540 --> 00:30:21,700 +اللي هي الـ Bordetella Pertussis تمام؟ إيش اللي عامل + +398 +00:30:21,700 --> 00:30:26,660 +المشترك بين التلاتة هدول اللي هم الـ Bordetella و + +399 +00:30:26,660 --> 00:30:30,100 +الـ Diphtheria و الـ Tetanus أن التلاتة الـ + +400 +00:30:30,100 --> 00:30:34,200 +Pathogenesis تبعهم معتمد على الـ exotoxin اللي + +401 +00:30:34,200 --> 00:30:37,640 +حولناه toxoid و أعطيناه vaccine و أعطيناه vaccine + +402 +00:30:37,640 --> 00:30:43,020 +طيب في اسم جديد حلو اسمه Francisella مارش معناها + +403 +00:30:43,020 --> 00:30:43,300 +قبلين + +404 +00:30:52,440 --> 00:30:56,060 +الـ Francisella لها اسم مشهور Francisella Tularensis + +405 +00:30:56,060 --> 00:31:00,100 +وبتعمل + +406 +00:31:00,100 --> 00:31:09,780 +مرض اسمه هم الأرانب Rabbits + +407 +00:31:09,780 --> 00:31:13,780 +Fever إياكم + +408 +00:31:13,780 --> 00:31:18,400 +عم تظنوا أنه الأرانب الوديعة الجميلة يعني ما بتنقلش + +409 +00:31:18,400 --> 00:31:21,240 +أمراضها، قد تنقل أمراض من ضمنها الـ Francisella + +410 +00:31:27,960 --> 00:31:32,800 +الـ Gram-Positive Bacilli زي مثلا الـ Listeria مرة بقعنا الـ + +411 +00:31:32,800 --> 00:31:37,400 +Listeria مرة بقعنا الـ Listeria و أنا مرة رسمت على + +412 +00:31:37,400 --> 00:31:43,260 +السبورة هنا وقلتلكوا أن في بكتيريا بتدخل من خلية + +413 +00:31:43,260 --> 00:31:48,120 +إلى خلية فاكرينها بالـ Actin Rockets صواريخ الـ Actin + +414 +00:31:48,120 --> 00:31:52,260 +هذه هي الـ Listeria طيب احنا بدأنا بنحكي جراثيم اليوم + +415 +00:31:52,260 --> 00:31:57,330 +كل محاضراتنا جراثيم،بدأ أعطيكوا الآن قصة مرتبطة بالـ .. + +416 +00:31:57,330 --> 00:32:03,650 +بالـ Listeria عشان نعزز ثقافة البحث العلمي وكيفية + +417 +00:32:03,650 --> 00:32:09,950 +تفسير النتائج بعض الأحيان قرأت مقالين متضاربين + +418 +00:32:09,950 --> 00:32:13,510 +مقالين أقصر الأبحاث بحتان يعني واحد بيقول إن الـ + +419 +00:32:13,510 --> 00:32:16,810 +Listeria monocytogenes بتموت بالبسترة فاكرين البسترة + +420 +00:32:16,810 --> 00:32:21,930 +72 درجة مئوية لمدة 15 ثانية بسترة + +421 +00:32:24,120 --> 00:32:28,960 +الآن الباحث بيقول أنه بتموت بالـ Pasteurization الـ + +422 +00:32:28,960 --> 00:32:32,260 +Conclusion تبعته والتاني بيقول أنه لأ ما بتموتش بالـ + +423 +00:32:32,260 --> 00:32:38,600 +Pasteurization قرأت الباحثين مرارا ومتكررا طريقة + +424 +00:32:38,600 --> 00:32:43,440 +تقريبا نفس الطريقة في الآخر انتبهت لنقطة معينة، أن + +425 +00:32:43,440 --> 00:32:46,920 +الباحث الأول بيقول أنا أخدت العينة اللي اشتغلت + +426 +00:32:46,920 --> 00:32:49,920 +عليها عامة الـ testing تبعي عليها أخدتها من بقرة + +427 +00:32:49,920 --> 00:32:54,580 +مصابة بالليستيريا أنا أخدت حليب فيه جواته ليستيريا + +428 +00:32:54,580 --> 00:32:57,940 +الثاني لأ جاب ليستيريا اللي زارعها هو في التلاجة + +429 +00:32:57,940 --> 00:33:01,120 +وحطها في الحليب فإيش الفرق بين الاثنين أن هذا من + +430 +00:33:01,120 --> 00:33:07,120 +Original Source وهذا من طبق طبعا لما .. لما .. لما + +431 +00:33:07,120 --> 00:33:13,030 +اتبعت الموضوع هذا بدأت الخيوط تتبدل، أول حاجة من هنا + +432 +00:33:13,030 --> 00:33:17,390 +ناخد بعض العبار من الموضوع أن هاي فيها تتضارب في + +433 +00:33:17,390 --> 00:33:21,910 +نتائج الأبحاث أنتم في الكتب .. في الكتب غالبا + +434 +00:33:21,910 --> 00:33:27,790 +تجيكوا المعلومة المصفاة المنقاة بدون شوائب تمام؟ و + +435 +00:33:27,790 --> 00:33:30,790 +بتخيّلي لأن احنا لو .. لو كل مرة نقولكوا في دراسة + +436 +00:33:30,790 --> 00:33:32,930 +بتقول كده وفي دراسة بتقول كده ولا حد حافظ على أي + +437 +00:33:32,930 --> 00:33:36,550 +معلومة فبنضطر في الـ Textbook نعطيكوا المعلومات + +438 +00:33:36,550 --> 00:33:41,150 +اللي شبه المتفق عليها، بينما في واقع البحث العلمي + +439 +00:33:41,150 --> 00:33:45,370 +هتلاقي كثير معلومات في الـ Microbiology مختلفة عليها + +440 +00:33:45,370 --> 00:33:49,150 +قد يكون الاختلاف حقيقي وقد يكون الاختلاف غير + +441 +00:33:49,150 --> 00:33:51,770 +حقيقي زي المثال اللي أنا ذكرته قبل شوية اختلاف + +442 +00:33:51,770 --> 00:33:56,190 +غير حقيقي هذا اختلاف لأن الطريقة المتبعة في الشغل + +443 +00:33:56,190 --> 00:34:01,910 +كانت مختلفة وقدت إلى نتائج مختلفة الـ Science يعرف + +444 +00:34:01,910 --> 00:34:05,410 +عليه أنه a field of study that deals with + +445 +00:34:08,150 --> 00:34:11,330 +interpretation of reproducible كلمة Reproducible + +446 +00:34:11,330 --> 00:34:15,030 +هي دي Key Word وحطيتها مليون خط Reproducible facts + +447 +00:34:15,030 --> 00:34:20,370 +البحث أو الـ Experiment لازم تطلع نفس النتيجة + +448 +00:34:20,370 --> 00:34:23,750 +Reproducible يعني كل مرة بنعيدها بتعطينا نفسها + +449 +00:34:23,750 --> 00:34:27,190 +النتيجة يده بتبقى على نفس الطريقة لكن الطريقة هي + +450 +00:34:27,190 --> 00:34:30,350 +اللي حصل فيها الأن Somewhere اختلف فبندرس الدرس + +451 +00:34:30,350 --> 00:34:35,970 +الثاني أن كيف نفسر الاختلافات، مش دائما نقول والله + +452 +00:34:35,970 --> 00:34:41,070 +واحد منهم إيه، غشاش، على طول نفتي أو نحكم بدون + +453 +00:34:41,070 --> 00:34:47,170 +مرجعية علمية، اقع جيدا، حاول تطلع على أي فروقات، + +454 +00:34:47,170 --> 00:34:51,210 +شوف الـ Temperature احنا في الأبحاث لما بنشر الأبحاث + +455 +00:34:51,210 --> 00:34:54,330 +بنحط بين قوسين الشركة اللي حطينا فيها الـ Incubator + +456 +00:34:54,330 --> 00:34:58,290 +الـ Incubator اللي مختلف من شركة لأخرى ممكن يكون دقة + +457 +00:34:58,290 --> 00:35:01,910 +الـ Incubator هي اللي اختلفت لما أنا بقول عزلة + +458 +00:35:01,910 --> 00:35:04,210 +Salmonella في غزة على سبيل المثال على سبيل الممارسة + +459 +00:35:04,210 --> 00:35:07,750 +الحقيقية 5% وسمين اللي يقولي قول عزلة عشرين في 10 + +460 +00:35:07,750 --> 00:35:11,650 +% عارفين الـ Incubation Temperature لو ارتفعت نصف + +461 +00:35:11,650 --> 00:35:14,410 +درجة قد تؤثر على الـ Frequency والـ Isolation؟ + +462 +00:35:15,230 --> 00:35:18,130 +فبالتالي اللي أنا بدي أقوله من .. من .. من الـ .. + +463 +00:35:18,130 --> 00:35:20,450 +الـ .. الـ .. الجزئي اللي .. أو الـ مثال اللي حكيته + +464 +00:35:20,450 --> 00:35:25,490 +أنه بيكون المقدرة على تفسير الاختلافات ما بين + +465 +00:35:25,490 --> 00:35:29,150 +الحقائق والمعلومات المتوفرة لدينا في السوق مثال + +466 +00:35:29,150 --> 00:35:35,670 +ثاني كنا بنعمل حقيقة معروفة اسمها Urinary Tract + +467 +00:35:35,670 --> 00:35:40,670 +Infection it is more common in females than males + +468 +00:35:40,670 --> 00:35:49,770 +بيبقى واحد بيفسر، ممكن يفسر مليون تفسير، منطقي، + +469 +00:35:49,770 --> 00:35:53,290 +تفسيرات منطقية، لكن مش بالضرورة يكون التفسير إيه + +470 +00:35:53,290 --> 00:35:58,150 +صحيح، وأعطيكم مثال على التفسير المنطقي والتفسير + +471 +00:35:58,150 --> 00:36:02,430 +الصحيح، ثلاثة فلاحين مروحين من الشغل، تبقى هم كل + +472 +00:36:02,430 --> 00:36:05,590 +واحد حاط طريقه على كده، تعبوا في الطريق، اللي + +473 +00:36:05,590 --> 00:36:10,350 +أجلوا محطة انتظار البساطي على تحتها، فجأة واحد + +474 +00:36:10,350 --> 00:36:14,900 +مات إيده هيك، قال إيه؟ الدنيا بتمطر، سأل سؤال قال + +475 +00:36:14,900 --> 00:36:18,620 +What causes the rain؟ ما الذي يسبب المطر؟ واحد + +476 +00:36:18,620 --> 00:36:25,220 +منهم قال في في السماء صناديق ولما الإله بده ينزل + +477 +00:36:25,220 --> 00:36:31,760 +المطر بيأمر الريح تجيب له الصناديق، الثاني قال لأ مش + +478 +00:36:31,760 --> 00:36:37,550 +صناديق فيه إسفنج، البخار بيطلع من الأرض .. من كوكب + +479 +00:36:37,550 --> 00:36:41,390 +الأرض والإسفنج بينطسه ولما الإله بده ينزل المطر + +480 +00:36:41,390 --> 00:36:46,190 +بيبقى ينريح بيعصر الإسفنج بينزل المطر، الثالث قال + +481 +00:36:46,190 --> 00:36:54,010 +لأ الماء بتتبخر وبيطلع فوق بيلاقي هواء بارد بتتكثف + +482 +00:36:54,010 --> 00:36:57,750 +البرمجة التي عرفناها الآن، السؤال اللي أنا هطلعه + +483 +00:36:57,750 --> 00:37:02,410 +عليكم مين منهم فسر موضوع المطر؟ الثالث .. الثالث + +484 +00:37:02,410 --> 00:37:02,850 +فسر + +485 +00:37:23,040 --> 00:37:27,740 +الثلاثة حاولوا وفسروا نزول المطر لكن السؤال Which + +486 +00:37:27,740 --> 00:37:32,230 +of that explanation was tested؟ الأخير اللي هو + +487 +00:37:32,230 --> 00:37:34,830 +اعملولي Testing على البراد الشاي لما حطي عليه + +488 +00:37:34,830 --> 00:37:38,750 +الجزاز واتكلمت عليه ونزل مطر، مضطر لأ؟ فاللي + +489 +00:37:38,750 --> 00:37:42,470 +بتقوله أنا أنه في الـ Science we accept logical + +490 +00:37:42,470 --> 00:37:48,170 +Logical explanation، تفسيرات منطقية، احنا خرجنا عن + +491 +00:37:48,170 --> 00:37:52,650 +الموضوع خالص لكن لأنه أنا معني ولأنه احنا في علم + +492 +00:37:52,650 --> 00:37:55,110 +اسمه علم الـ Microbiology اللي بندرسه كثير من + +493 +00:37:55,110 --> 00:37:58,230 +المعلومات اللي هنجيبها بعد ما نخلص هنجيبها في بعض + +494 +00:37:58,230 --> 00:38:00,730 +الأحيان متضاربة فبنكون عندي المقدرة وبداية + +495 +00:38:00,730 --> 00:38:05,030 +المقدرة أنه أتقبل هذا الاقتراح بسبب إيش قلناها؟ + +496 +00:38:05,360 --> 00:38:08,760 +بسبب أنه ممكن يكون Different Methodologies أو + +497 +00:38:08,760 --> 00:38:11,000 +Different Sample Sites أنا اشتغلت على تلاتمية و + +498 +00:38:11,000 --> 00:38:14,600 +اشتغل على خمسمية أنا اشتغلت في منطقة جغرافية ضيقة + +499 +00:38:14,600 --> 00:38:18,000 +واشتغل في منطقة جغرافية واسعة، المنطقة الجغرافية + +500 +00:38:18,000 --> 00:38:23,980 +مصر غير عن غزة، الأردن غير عن سوريا، أمريكا غير، + +501 +00:38:23,980 --> 00:38:27,920 +الديانة بتفرق، ال .. ال .. ال .. نعم بتأثر على + +502 +00:38:27,920 --> 00:38:31,100 +نتاجنا، احنا بلا نصب .. بلا صعب بيه الديدان اللي في + +503 +00:38:31,100 --> 00:38:33,960 +التقرع عن طريق الخنزير لإن احنا مسلمين، فلو عملنا + +504 +00:38:33,960 --> 00:38:37,380 +في .. في community في أمريكا مسلمة، ولا لإن عندهم + +505 +00:38:37,380 --> 00:38:40,280 +zero دودة شريطية ونقول إن أمريكا مفيش فيها دودة + +506 +00:38:40,280 --> 00:38:42,440 +شريطية، ما احنا .. احنا .. لإن احنا عملنا + +507 +00:38:42,440 --> 00:38:46,660 +community ..مسلمة، فالقصد من الكلام أنه بيكون عن + +508 +00:38:46,660 --> 00:38:51,340 +المقدرة على أنه إيه أقوم ببعض التفسيرات للاختلافات، + +509 +00:38:51,340 --> 00:38:54,420 +الجزء الأخير من الجدول هو عبارة عن ال + +510 +00:38:54,420 --> 00:38:57,700 +enterobacteriaciae زي الإشريشيا، السلمونيلا، + +511 +00:38:57,700 --> 00:39:02,040 +الشيجيلا، اليارسينيا، مارعكوا اليارسينيا قبل هيك؟ + +512 +00:39:02,040 --> 00:39:02,720 +يارسينيا + +513 +00:39:07,770 --> 00:39:10,790 +في الـ enterobacter و الـ protease و الـ ceratia و الـ + +514 +00:39:10,790 --> 00:39:13,290 +edwards اللي لكل مدولة بنعتبرهم احنا + +515 +00:39:13,290 --> 00:39:18,490 +enterobacterias فينا ميكروبات أخرى زي الـ vibrio و + +516 +00:39:18,490 --> 00:39:21,190 +الـ hemophilus الـ vibrio طبعا مشهورة الـ vibrioplera + +517 +00:39:21,190 --> 00:39:26,410 +الـ hemophilus influenza الباستوريلا اللي كانت اللي + +518 +00:39:26,410 --> 00:39:30,870 +جرسينا فيها يوم من ايام تسمى pastorella Legionella + +519 +00:39:30,870 --> 00:39:35,010 +حلو هذا الـ Legionella اسم برضه هيمور معانا إن شاء + +520 +00:39:35,010 --> 00:39:38,520 +الله بعدين من الميكروبات الأولوية التانية اللي هي + +521 +00:39:38,520 --> 00:39:42,720 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +522 +00:39:42,720 --> 00:39:43,840 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +523 +00:39:43,840 --> 00:39:43,880 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +524 +00:39:43,880 --> 00:39:48,760 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +525 +00:39:48,760 --> 00:39:48,920 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +526 +00:39:48,920 --> 00:39:49,460 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +527 +00:39:49,460 --> 00:39:49,520 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +528 +00:39:49,520 --> 00:39:51,720 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +529 +00:39:51,720 --> 00:39:51,780 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +530 +00:39:51,780 --> 00:39:51,840 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +531 +00:39:51,840 --> 00:39:51,900 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +532 +00:39:51,900 --> 00:39:54,180 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +533 +00:39:54,180 --> 00:39:57,800 +ال .. + +534 +00:39:57,800 --> 00:39:59,380 +ال + +535 +00:40:06,970 --> 00:40:11,570 +و الـ Bartonella و الـ Clamydia و الـ Rickettsia و الـ + +536 +00:40:11,570 --> 00:40:16,550 +Mycoplasma في طبعا بعض الميكروبات الأخرى اللي احنا + +537 +00:40:16,550 --> 00:40:19,830 +ما ذكرناها غالبا هي أقل أهمية من الميكروبات + +538 +00:40:19,830 --> 00:40:20,250 +التالية diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/gejJKbVhKOc_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/gejJKbVhKOc_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..b72988223364b98d258550748fda977dc32229df --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/gejJKbVhKOc_postprocess.srt @@ -0,0 +1,2152 @@ +1 +00:00:21,720 --> 00:00:23,480 +أهلا و رحبا الحمد لله و رحمة الله و صلاة والسلام + +2 +00:00:23,480 --> 00:00:26,500 +على سيدنا محمد و على قليه و صحبه و من سرع بيه + +3 +00:00:26,500 --> 00:00:30,740 +اليوم الدين أما بعد هذه المحاضرة هي الأخيرة في + +4 +00:00:30,740 --> 00:00:35,020 +الجزء المتعلق بال Virus Genes و ال Immunity لل + +5 +00:00:35,020 --> 00:00:39,100 +bacterial infection هتكلم اليوم عن ال origin and + +6 +00:00:39,100 --> 00:00:43,630 +development of bacterial infectionsفي هنا مصطلحين + +7 +00:00:43,630 --> 00:00:47,530 +بنطلع عليهم بشكل دقيق المصطلح الأول بيقول ان ال + +8 +00:00:47,530 --> 00:00:51,770 +sources of infection اما indigenous او exogenous + +9 +00:00:51,770 --> 00:00:55,530 +يعني يا اما مصدر داخلي يا اما مصدر خارج من اين + +10 +00:00:55,530 --> 00:00:59,310 +اتقت المصادر الداخلية للعدوى من ال normal + +11 +00:00:59,310 --> 00:01:03,830 +microbiota اللي مولودة عندنا كلكم تتذكروا ان احنا + +12 +00:01:03,830 --> 00:01:08,090 +قسمنا الدسم الى تسمية استرال ونسترال كل الأجزاء + +13 +00:01:08,090 --> 00:01:13,270 +اللي نسترال زي الجاس بردسنالتراليتمام؟ ده ال + +14 +00:01:13,270 --> 00:01:16,970 +vagina هذه تعتبر و ال skin تعتبر sources of + +15 +00:01:16,970 --> 00:01:22,370 +indigenous اللي هو infection الأدل exogenous هي + +16 +00:01:22,370 --> 00:01:26,430 +الكثيرة و المتنوعة اللي مثلا على سبيل المثال ممكن + +17 +00:01:26,430 --> 00:01:30,570 +ناخدها من ال environment من ال air من ال surfaces + +18 +00:01:30,570 --> 00:01:35,470 +تمام؟ من ال close contact مع الآخرين من الماء اللي + +19 +00:01:35,470 --> 00:01:38,290 +بنتناوله او من الغذاء اللي احنا بنتناوله ده + +20 +00:01:38,290 --> 00:01:39,210 +بيسميها ايه؟ + +21 +00:01:45,230 --> 00:01:47,470 +الـ Cycle دا يا عزيزي هي Cycle يريد نضالها و + +22 +00:01:47,470 --> 00:01:56,390 +نرجعلها طول فترة الحديث Cycle بتلخص لنا سلسلة + +23 +00:01:56,390 --> 00:02:01,870 +الأحداث المتعلقة بأي Infection في الدنيا والسلسلة + +24 +00:02:01,870 --> 00:02:03,550 +دي اللي عملتها باللغة العربية اللي عملتها في + +25 +00:02:03,550 --> 00:02:07,530 +محاضرة أعتقد لمستوى أقل من مستواكه لكن في الآخر هي + +26 +00:02:07,530 --> 00:02:11,490 +تعبر عن الواقع ال .. ال .. ال .. Infection Cycle + +27 +00:02:11,490 --> 00:02:14,350 +بنبدأ بالعمل المسبب + +28 +00:02:18,370 --> 00:02:23,850 +يكون بكتيريا قد يكون فيروس قد يكون فنجال قد يكون + +29 +00:02:23,850 --> 00:02:28,010 +برزات و البرزات قد تكون ديدان او قد تكون بروتوزروف + +30 +00:02:28,010 --> 00:02:33,090 +لكن في الآخر هي ال pathogens الان اي pathogen في + +31 +00:02:33,090 --> 00:02:35,390 +الدنيا اللي هو ما يسمى بال reservoir و reservoir + +32 +00:02:35,390 --> 00:02:40,750 +يعني مخازن مخزن للعدوى اعطوك مثال على مخازن يعني + +33 +00:02:40,750 --> 00:02:46,410 +ايش يعني مخازن للعدوى انسان مصعببفيروس الرشح هو + +34 +00:02:46,410 --> 00:02:49,930 +مخزن لفيروس الرشح لأنه كل ما بيعتص هو بيطلع + +35 +00:02:49,930 --> 00:02:55,170 +الفيروس فهو مخزن للعدوى تمام؟ قد يكون المخزن + +36 +00:02:55,170 --> 00:03:00,350 +الإنسان المصعب، قد يكون الإنسان الحامل للمرض مخزن، + +37 +00:03:00,350 --> 00:03:03,430 +قد تكون بعض الحشرات مخزن، قد تكون البيئة نفسها + +38 +00:03:03,430 --> 00:03:08,450 +مخزن، قد تكون حيات أخرى مخزن، يبقى فيها أنها مخزن، + +39 +00:03:08,450 --> 00:03:14,100 +بس إيش هو يا مرتبط مين؟ باسم ال pathogen نفسهالان + +40 +00:03:14,100 --> 00:03:17,980 +احنا قلنا ان الإنسان المصاب مخزن صح ولا لأ عشان ال + +41 +00:03:17,980 --> 00:03:22,420 +infection تحدث لازم يخرج الميكروب من المخزن لازم + +42 +00:03:22,420 --> 00:03:27,820 +يكون له portal of exit مكان خروج تبقى أماكن الخروج + +43 +00:03:27,820 --> 00:03:33,300 +زي مثلا زي ال secretions الصديدية + +44 +00:03:33,300 --> 00:03:36,300 +على سبيل المثال واحد عنده دمل مش هدا infection و + +45 +00:03:36,300 --> 00:03:42,240 +pathogen الصديد اللي بيخرج هذا هومن الجن طلع + +46 +00:03:42,240 --> 00:03:47,660 +portal of exit وحد عنده nasal secretions يعني + +47 +00:03:47,660 --> 00:03:53,380 +إفرازات من الأنف هذه برضه عبارة عن exit ال stool + +48 +00:03:53,380 --> 00:03:57,820 +ال urine عبارة عن portal of exit إذا المتفقين على + +49 +00:03:57,820 --> 00:04:01,740 +أن الباطوجين to make an infection it should have + +50 +00:04:01,740 --> 00:04:09,490 +an exit طيب إذا خرج بده كمان طريقة انتقالوطرق + +51 +00:04:09,490 --> 00:04:14,070 +الانتقال عديدة ومتنوعة مش هتطرقلها دلوقت لكن بدنا + +52 +00:04:14,070 --> 00:04:20,130 +نتخيل ان ميكروب خرج الى البيئة للماء مثلا ولم + +53 +00:04:20,130 --> 00:04:24,810 +يشربه أحد يبقى انكسرت ال cycle ودلوقت ما صار + +54 +00:04:24,810 --> 00:04:28,610 +infection بعد فترة هيموت ال .. ال .. ال .. ال + +55 +00:04:28,610 --> 00:04:33,610 +pathogen تمام؟ إذا لازم يكون له طريق انتقال في بعض + +56 +00:04:33,610 --> 00:04:37,050 +طرق الانتقال على سبيل المثال قد لا تكون تقليدية + +57 +00:04:37,830 --> 00:04:45,730 +حشرة تاخد عينة عين الدم من مريض فتشكله exit بتشكل + +58 +00:04:45,730 --> 00:04:49,950 +لل pathogen exit من مين؟ من المخزن المصاب صح؟ و قد + +59 +00:04:49,950 --> 00:04:55,030 +تكون أيضا هي اللي تاخد وجبة من إنسان سليم فتعمله + +60 +00:04:55,030 --> 00:05:00,190 +طريقة نقل طريقة نقل إذا هناك طرق نقل متنوعة منها + +61 +00:05:00,190 --> 00:05:08,020 +الحشرة طيب طريقة النقل احنا كناممكن تكون عبر منافذ + +62 +00:05:08,020 --> 00:05:13,800 +مختلفة زي المخارج ما كانت مختلفة المداخل واخدوا + +63 +00:05:13,800 --> 00:05:18,220 +بالكم معظم الأحيان نفس المنافذ اللي هي المداخل هي + +64 +00:05:18,220 --> 00:05:24,760 +نفس من المخارج الان دخل الميكروب دخل وين؟ لازم + +65 +00:05:24,760 --> 00:05:28,680 +يدخل في عائل قابل للإصابة وبنسميها باللغة الإلمية + +66 +00:05:28,680 --> 00:05:33,970 +susceptible host يعني ايش ان عائل قابل للإصابة؟قبل + +67 +00:05:33,970 --> 00:05:37,570 +كان محاضرة اتكلمنا عن tissue و species tropicalism + +68 +00:05:37,570 --> 00:05:42,330 +او ال tissue tropicalism و species specificity لما + +69 +00:05:42,330 --> 00:05:46,690 +كنا إذا العائل مالوش receptor للبعض و جين يرتبط من + +70 +00:05:46,690 --> 00:05:49,830 +خلالها للبعض و جين ما تشايفها ال infection يبقى مش + +71 +00:05:49,830 --> 00:05:53,250 +كل مايكروب بدخل في أيسامنا بيعمل infection لازم + +72 +00:05:53,250 --> 00:05:58,230 +نكون احنا susceptible له قابلين للإصابة بهذا ال + +73 +00:05:58,230 --> 00:06:03,330 +micro إذا بدي host قابل للإصابةطبعا ال host القابل + +74 +00:06:03,330 --> 00:06:07,930 +للإصابة سوف يصاب و هيمل تكاثر لمين؟ لل pathogen، + +75 +00:06:07,930 --> 00:06:10,910 +هيك اكتملت ال cycle اللي .. ليش بندرس هذا ال cycle + +76 +00:06:10,910 --> 00:06:15,890 +يعني؟طب عشان انا اجرب الموضوع اكتر بإنه نرجع + +77 +00:06:15,890 --> 00:06:18,650 +لدكتور عدنان الهندي في التوفايل يعني دكتور عدنان + +78 +00:06:18,650 --> 00:06:22,410 +بدرسنا و بصر علينا انه نحفظ ال life cycle تبع ال + +79 +00:06:22,410 --> 00:06:26,730 +Ascaris و ال Intamina و ال Protozoa و كل الديدان + +80 +00:06:26,730 --> 00:06:32,430 +لإن هو عارف أهمية ال life cycle ان وين بنجوسها if + +81 +00:06:32,430 --> 00:06:35,330 +نمنع ال transmission طب تعالوا نطلع على ال cycle + +82 +00:06:35,330 --> 00:06:40,030 +لإن هذه مابه cycle لو أنا بدي أمنع ال cycle of + +83 +00:06:40,030 --> 00:06:45,430 +infection بدي أحارب المخازنمخزن، كل إنسان مصاب + +84 +00:06:45,430 --> 00:06:49,510 +بيتدور عليهم، إنسان مصاب بده يعالجه، إنسان career + +85 +00:06:49,510 --> 00:06:53,550 +بده يعالجه، تمام؟ بده عزله، مش ضروري أعالجه، + +86 +00:06:53,550 --> 00:06:57,030 +عزلته، يعني في actions متنوعة و مختلفة قد أتخذها + +87 +00:06:57,030 --> 00:07:00,450 +لمن اللي هو العدو، المخارج صعب التحكم فيها لكن + +88 +00:07:00,450 --> 00:07:05,140 +ممكن يكون إشراقات، يا مريض ألبس كمامةيا مستقبل + +89 +00:07:05,140 --> 00:07:09,080 +ألبس كمانة تمام؟ يبقى فيها أنها إمكانية الأدوات + +90 +00:07:09,080 --> 00:07:12,600 +اللي استخدمناها وممكن تلاوس ال exits عقّمها يوم + +91 +00:07:12,600 --> 00:07:15,240 +انت خلص منها ده ال environment هاي كمان وسيلة من + +92 +00:07:15,240 --> 00:07:18,980 +الوسائل طرق النقل احنا جولناها حشرات مثلا بنقول + +93 +00:07:18,980 --> 00:07:23,880 +طرق النقل إذا جولنا إن الحشرة X بتسبب أو بتنقل مرض + +94 +00:07:23,880 --> 00:07:29,300 +معين كفه الحشرات بدل من كافح من أدمين بنكافح من + +95 +00:07:29,300 --> 00:07:33,220 +الحشرات يبقى عند بطل على السلسلة + +96 +00:07:36,970 --> 00:07:41,350 +هو أكتر فعالية من الطرق وبستخدمها لمنع اللي هو ال + +97 +00:07:41,350 --> 00:07:46,470 +infection cycle إذا ال chain of transmission أو + +98 +00:07:46,470 --> 00:07:50,250 +سلسلة الانتقال موضحة في أكم أنصر بال pathogen بال + +99 +00:07:50,250 --> 00:07:55,130 +reservoir بال exit بال transmission بال portal of + +100 +00:07:55,130 --> 00:08:00,990 +entry وب susceptible host أنصر قابل أو عائل قابل + +101 +00:08:00,990 --> 00:08:03,850 +للإصابة بالنسبة لل transmission اللي حاطينه هنا + +102 +00:08:03,850 --> 00:08:06,370 +مجموعة من الطرق زي مثلا airborne + +103 +00:08:11,870 --> 00:08:14,250 +المراتب المنتقلة عن طريق اللي هو الغذاء و عن طريق + +104 +00:08:14,250 --> 00:08:16,690 +ال water و انا هحطي نموذج احنا اللي هو sexual + +105 +00:08:16,690 --> 00:08:24,910 +contact roots of infection مداخل اللي هو او من اين + +106 +00:08:24,910 --> 00:08:28,410 +تأتي او من اين تدخل الميكروبات عبر ال respiratory + +107 +00:08:28,410 --> 00:08:31,370 +tract و انا بتقولكم المرة اذكركم ان ال portals of + +108 +00:08:31,370 --> 00:08:34,550 +entry هم تقريبا نفسهم ال portal of exit + +109 +00:08:37,250 --> 00:08:41,910 +عطس، كحة، كل هذه ممكن تنقل إيه؟ Troubles، وتخيلوا + +110 +00:08:42,820 --> 00:08:47,320 +العطسة الواحدة بتطلع ملايين .. ملايين ال particles + +111 +00:08:47,320 --> 00:08:52,080 +تمام؟ جوا كل particle قد تحمل فيروس أو بكتيريا + +112 +00:08:52,080 --> 00:08:56,220 +تمام؟ و بتنشرها و قد تصل المسافة القضيفة اللي + +113 +00:08:56,220 --> 00:08:59,320 +خارجها من الأنف إلى مسافة متر و نصف عشانك احنا + +114 +00:08:59,320 --> 00:09:02,500 +بنقول على الأقل اللي بدك بينك و بين ال .. الآخر + +115 +00:09:02,500 --> 00:09:05,760 +يعني في .. لمانها الأكبر إنك مسافة أكتر من متر + +116 +00:09:05,760 --> 00:09:09,220 +إيه؟ و نصف عتابة من مصطلح القضيفة يعني احنا .. + +117 +00:09:09,220 --> 00:09:11,520 +احنا بتاخد مصطلحاتنا من بيئتنا يعني من أين نجيبها + +118 +00:09:13,890 --> 00:09:17,370 +بتقلل نعم بتقلل من المسافة لكن هذا لا يمنع + +119 +00:09:17,370 --> 00:09:21,950 +الانتشار لإنه حتى الإيدين مش completely closed + +120 +00:09:21,950 --> 00:09:24,490 +يعني المناطق و هو هيخرج من هنا و من هنا كمان لكن + +121 +00:09:24,490 --> 00:09:28,870 +بيقلل الكمية gastroenteric + +122 +00:09:28,870 --> 00:09:32,150 +عبر اللي هو ال gastrointestinal tract water or + +123 +00:09:32,150 --> 00:09:35,830 +food genitourinary tract اللي هي القنال البولية + +124 +00:09:35,830 --> 00:09:40,450 +الجنسية close contact الاتصال المباشر insect bite + +125 +00:09:40,450 --> 00:09:48,600 +عضات الحشراتالحصات غالبا بتستخدمنا لوجبة دم زي + +126 +00:09:48,600 --> 00:09:54,460 +البعوض أو الحشاطات زي ال bugs أو الفليز bugs اللي + +127 +00:09:54,460 --> 00:09:59,480 +هي ال بق بلغة عربية blood transfusion و ده ايه + +128 +00:09:59,480 --> 00:10:02,300 +طبعا يعني دخيلة على الطب ماكانش مولود على طريقة + +129 +00:10:02,300 --> 00:10:06,180 +كان زمان يعني جاب المئة عامة كانت حد اسمها blood + +130 +00:10:06,180 --> 00:10:08,700 +transfusion لكن الآن صارت جزء من ال medical + +131 +00:10:08,700 --> 00:10:15,360 +practicesخدوا بالكوا كتير من الإجراءات الطبية قد + +132 +00:10:15,360 --> 00:10:20,440 +تسبب لنا infection وهذه واحدة من النقاط اللي .. + +133 +00:10:20,440 --> 00:10:24,460 +لما احنا بنقول hospital acquired infection جداش + +134 +00:10:24,460 --> 00:10:27,980 +الإنسان بيتعرض لإجراءات طبية، كام قبر بياخد في + +135 +00:10:27,980 --> 00:10:32,380 +المستشفى، كام canula، كام جراحة، جداش بزيد فرص ان + +136 +00:10:32,380 --> 00:10:37,480 +يصيب بـinfectionال parental roots و عن طريق الحقن + +137 +00:10:37,480 --> 00:10:40,780 +ال mucous membranes الأشياء المخاطية و أنا مرة + +138 +00:10:40,780 --> 00:10:44,160 +ذكرتلكوا ال finger to eye فاكرينها؟ finger to eye + +139 +00:10:44,160 --> 00:10:46,980 +أحد الأشياء المهمة جدا في موضوع اللي هو ال + +140 +00:10:46,980 --> 00:10:52,530 +transmissionطيب we are going to classify infection + +141 +00:10:52,530 --> 00:10:57,050 +according to infectious state الحالة اللي بيمر + +142 +00:10:57,050 --> 00:11:01,650 +فيها المريض نفسه بتساعدنا في تصنيف أنواع من العدوى + +143 +00:11:01,650 --> 00:11:05,310 +مثلا بقولنا في نوع من العدوى اسمه in apparent و in + +144 +00:11:05,310 --> 00:11:10,050 +apparent عكس apparent apparent معناها ظاهر واضح + +145 +00:11:10,050 --> 00:11:15,890 +أمام بينما in apparent غير واضح إلا اسم تاني أشهر + +146 +00:11:15,890 --> 00:11:19,970 +منها اسمه subclinical infectionsubclinical يعني + +147 +00:11:19,970 --> 00:11:25,570 +صار في عدوى وصار فيه تكاثر للبكتيريا لكن لم يظهر + +148 +00:11:25,570 --> 00:11:30,750 +هناك أي أعراض عشان تسميها subclinical infection + +149 +00:11:30,750 --> 00:11:35,730 +رقم اتنين latent infection وهذا مختلف تماما عن ال + +150 +00:11:35,730 --> 00:11:39,330 +inapparent أو ال subclinical خدوا مثال على ال + +151 +00:11:39,330 --> 00:11:43,890 +latent infection عشان بحلزمنا كمان شوية بعض البعض + +152 +00:11:43,890 --> 00:11:47,730 +مننا وانا منهم أصاب بحمل بعد + +153 +00:11:50,130 --> 00:11:53,930 +هذا الحم هو مش حم بمفهوم انه من حرارة الجسم هذا + +154 +00:11:53,930 --> 00:11:57,290 +عبارة عن viral infection اسمها herpes simplex type + +155 +00:11:57,290 --> 00:12:00,890 +1 الان + +156 +00:12:00,890 --> 00:12:07,130 +مش موجود لكن هو موجود موجود و مقتبل بينتظر فرصة + +157 +00:12:07,130 --> 00:12:13,570 +تهيئة الظروف المناسبة له مثلا اصاب ب influenza مرة + +158 +00:12:13,570 --> 00:12:19,400 +اخرى انا فالجسم انا اعتباره بضعفبسبب ال dryness + +159 +00:12:19,400 --> 00:12:23,280 +بعض الأحيان جفاف ففيه حاجة باسمها inciting factors + +160 +00:12:23,280 --> 00:12:29,400 +عوامل بتحفظه على الخروج لكن كلها مرتبطة بإن + +161 +00:12:29,400 --> 00:12:34,400 +المناعة تكون منخفضة عند الشخص الأقل عندي ماعنديش + +162 +00:12:34,400 --> 00:12:39,840 +لكن موجود أو مش موجود هو موجود هذا بيموت معانايعني + +163 +00:12:39,840 --> 00:12:44,080 +يبتسم مرة واحدة على سبيل المثال تمام؟ ويبقى ال + +164 +00:12:44,080 --> 00:12:46,980 +virus اللي بحكي عنه اللي هو ال Herpes Simplex في + +165 +00:12:46,980 --> 00:12:52,480 +virus تاني بتسمعوا فيه Varicella Zoster Varicella + +166 +00:12:52,480 --> 00:12:55,920 +Zoster Varicella اللي هي ال chicken box جدر الميه + +167 +00:12:55,920 --> 00:12:59,160 +اللي انتوا تعرفوه جدر الميه اسمه بالانجليزية + +168 +00:12:59,160 --> 00:13:04,100 +chicken box بينصب فيه غالبا احنا الصغار بعد تلاتين + +169 +00:13:04,100 --> 00:13:08,590 +سنة اربعين سنة خلاص طبعا احنا بنطيب منهبعد ترديس + +170 +00:13:08,590 --> 00:13:13,710 +الاربعين سنة بنلاجأ طلعنا حب أو blisters على .. في + +171 +00:13:13,710 --> 00:13:17,890 +الظهر، بيسموها الحزام الناري، تسمعوا فيك، الحزام + +172 +00:13:17,890 --> 00:13:23,490 +الناريهذا الحزام الناري هي عبارة عن activation او + +173 +00:13:23,490 --> 00:13:27,130 +reactivation ل latent infection بالفارسيلا دخل + +174 +00:13:27,130 --> 00:13:34,150 +فارسيلا و طلع زوستا بعد كام سنة يعني عمل latency + +175 +00:13:34,150 --> 00:13:38,350 +period لكن النقطة الحلوة في الموضوع هنا اللي بدنا + +176 +00:13:38,350 --> 00:13:43,950 +نفهمها انه غير معدي لما بيكون latent لما بيكون + +177 +00:13:43,950 --> 00:13:48,480 +latent بيكون غير معدي كامن ساكنما بيتكاتر بتالي + +178 +00:13:48,480 --> 00:13:52,200 +غير معدى ليش ذكرت المعلومة دى عشان انا .. عشان + +179 +00:13:52,200 --> 00:13:55,620 +اميزها عن رقم اربعة رقم اربعة اللى هى ال career + +180 +00:13:55,620 --> 00:14:04,480 +state حامل للمرض حامل وبينشر ال micro وبينشر ال + +181 +00:14:04,480 --> 00:14:10,040 +micro بينما الأولارش كان كامن ومش معدى احكيكوا قصة + +182 +00:14:10,040 --> 00:14:15,890 +عن ال career state فى واحدة اسمها Maryاشتهرت + +183 +00:14:15,890 --> 00:14:19,890 +تاريخيا في الطب باسم tayfoy and married، ما أسوأ + +184 +00:14:19,890 --> 00:14:24,310 +إن الإنسان يتلقى بـ tayfoy، بسلمونيلا تايفي، تمام؟ + +185 +00:14:25,200 --> 00:14:28,360 +هذه قصة مشهورة زي اللي عملت جوجلينج عليها هتشوفوها + +186 +00:14:28,360 --> 00:14:32,440 +على الانترنت بشكل يعني كتير مبتوب عنها هذه سيدة + +187 +00:14:32,440 --> 00:14:37,160 +فقيرة كانت تعمل في بريطانيا وكانت حامل لميكروب + +188 +00:14:37,160 --> 00:14:42,720 +السلمونيلاتايفين حامل بالميكروب وليست لا يقضي + +189 +00:14:42,720 --> 00:14:45,520 +عليها أي أعراض والدنيا لو عندها أعراض كان مشتغلة + +190 +00:14:45,520 --> 00:14:50,620 +كانت بصحة جيدة وكانت تعمل في أعمال وضيع منها الطهي + +191 +00:14:50,620 --> 00:14:57,480 +والتنظيففي مؤسسات يعني أينما حلت ماري حلت tayfoyd + +192 +00:14:57,480 --> 00:15:01,000 +ده مؤشر على مستوى النظافة عندها كمان سيء مش بس + +193 +00:15:01,000 --> 00:15:03,620 +فقيرة بتشتغل في أعمال وضيعها كمان مستوى النظافة + +194 +00:15:03,620 --> 00:15:08,940 +عندها سيء لإنه من ال stool تبعها بينتقل للناس لإن + +195 +00:15:08,940 --> 00:15:11,580 +استعمال الدايفي بيصير الشدن في وين؟ في ال stool + +196 +00:15:11,580 --> 00:15:16,660 +المهم في كان طبيب بيتبع الوباء تبع tayfoyd في + +197 +00:15:16,660 --> 00:15:20,140 +بريطانيا وكان كل ما يصير في منطقة يروح يدرس + +198 +00:15:20,140 --> 00:15:20,660 +المنطقة + +199 +00:15:24,340 --> 00:15:29,960 +وصار فيها الوباء في سجن لجأ انه ماري تشتغل طبخة في + +200 +00:15:29,960 --> 00:15:35,940 +السجن طبعا فهرفه انه ال .. ال .. ال .. طبعا هي زي + +201 +00:15:35,940 --> 00:15:39,840 +ما قلتلكوا هي هذه ال career estate نموذج رائعة على + +202 +00:15:39,840 --> 00:15:43,200 +ال career estate انه هي لا تعاني من اي أعراض فكان + +203 +00:15:43,200 --> 00:15:46,340 +الصبح في سؤال عند الطلاب انه من الذي يجعل ال + +204 +00:15:46,340 --> 00:15:51,650 +microbe يعني يتخذ هذا الشكل من ال .. من الأشكالهو + +205 +00:15:51,650 --> 00:15:54,630 +غالبا بيكون عمل infection و عمل .. ممكن يكون عمل + +206 +00:15:54,630 --> 00:15:58,150 +disease كمان يا بنات و طابت منه .. او طابت منه + +207 +00:15:58,150 --> 00:16:02,390 +الشخص يعني .. شوف يا منه الشخص لكن وصل لمكان لا هو + +208 +00:16:02,390 --> 00:16:07,390 +جادر يتعامل بكميات كبيرة كاتر ولا هو .. ولا جهاز + +209 +00:16:07,390 --> 00:16:09,730 +الملاعق جادر يتخلص منه زي مثلا ما بيصير في ال + +210 +00:16:09,730 --> 00:16:12,670 +gallbladder بيصير الميكروبلم إيه للجوال؟ blood + +211 +00:16:13,820 --> 00:16:17,380 +الجورفدر تفرز bile salts، ال bile salts معروف عنها + +212 +00:16:17,380 --> 00:16:20,900 +أنها antimicrobial activity عالية، ثانوية اللي + +213 +00:16:20,900 --> 00:16:24,020 +مقاومة، بس مش مقاومة hundred percent، بمعنى أنها + +214 +00:16:24,020 --> 00:16:28,780 +بتموت، فبالتكاثر بيكون ضئيل، مش جادر تصل ال + +215 +00:16:28,780 --> 00:16:31,780 +numbers يعني يعمل disease، quantum sensing، + +216 +00:16:31,780 --> 00:16:35,360 +فاكرين؟ ولا جادر الجسم يتخلص منها لأنها موجودة في + +217 +00:16:35,360 --> 00:16:39,400 +مكان هي resistant نوعا ما كيلو أو أنها ممكن تتخذ + +218 +00:16:39,400 --> 00:16:43,890 +شكل normal microbiota في هذا الإنساناحنا مش كنا ال + +219 +00:16:43,890 --> 00:16:46,770 +microbiota بيصير فيها نوع من اتزان، فهي الورقة + +220 +00:16:46,770 --> 00:16:49,530 +اللي انا صارت متزينة مع مين، و السؤولي مكان انا + +221 +00:16:49,530 --> 00:16:52,470 +بتعيش بينكوا ك normal microbiota، مافي إمكانية أن + +222 +00:16:52,470 --> 00:16:56,010 +تصل لل number العادل تبعها، إذن ميزنا بال latent + +223 +00:16:56,010 --> 00:17:01,110 +infection وبين ال carrier stigmaرقم واحد ورقم + +224 +00:17:01,110 --> 00:17:05,110 +تلاتة عكس بعض تماما الأولانية inapparent التانية + +225 +00:17:05,110 --> 00:17:07,530 +apparent اللي هي ال disease اللي احنا بنشهر فيه + +226 +00:17:07,530 --> 00:17:12,670 +وبيظهر هنا أعراض واضحة لهذا المرض إذا inapparent + +227 +00:17:12,670 --> 00:17:16,310 +Latin ماحدش تقلّي بعدها كده إيش الفروقات بينهم + +228 +00:17:16,310 --> 00:17:19,230 +واضحين لإن الفروقات بينهم لإن apparent إنه أصلا + +229 +00:17:19,230 --> 00:17:23,470 +ماعرفناها احنا احنا ماشوفناها كيف عرفنا إنه مصاب؟ + +230 +00:17:23,470 --> 00:17:27,330 +ممكن نعمل فحص رلوجي نعرف إن في عنده antibodies لكن + +231 +00:17:27,330 --> 00:17:29,950 +هو لم يشهر ولم يظهر عليه + +232 +00:17:35,650 --> 00:17:40,210 +بنشوف الحم فيها بيشفق، بيختبئ ال .. ال microbe + +233 +00:17:40,210 --> 00:17:43,770 +لفترة معينة، إذا توفرته الظروف بيرجع مرة تانية، + +234 +00:17:43,770 --> 00:17:48,790 +لكن خلال فترة اختفاقه هو ليس مؤدي، ال apparent + +235 +00:17:48,790 --> 00:17:53,270 +infection كل الصفات موجودة فيها ال carrier state + +236 +00:17:53,270 --> 00:17:57,610 +هي صفة حامل للميكروب ولكن بيكون برضه أو عنده + +237 +00:17:57,610 --> 00:18:01,350 +القدرة أنه يعدي الآخر بس تحس يعني قصة الحم هي ممكن + +238 +00:18:01,350 --> 00:18:07,880 +تيجي من شخص لآخر؟عشان هيك اللي عنده ممنوع يجرب + +239 +00:18:07,880 --> 00:18:11,140 +الأطفال يعملوا kissing أو يلعبهم يعني يقبل الأطفال + +240 +00:18:11,140 --> 00:18:15,620 +تقبيل الأطفال من الأشياء اللي بتنقل ال micro .. ال + +241 +00:18:15,620 --> 00:18:18,900 +.. ال .. ال herpes بيسموها kissing disease لإنه + +242 +00:18:18,900 --> 00:18:21,740 +بينتقل عن طريق التقبيل وبالتالي الإنسان اللي عنده + +243 +00:18:21,740 --> 00:18:29,720 +حم يبتعد قدر الإمكان عن إيه؟ عن الأطفال طيب هذا + +244 +00:18:29,720 --> 00:18:32,700 +كان تصنيف infection state تصنيف على حسب ال + +245 +00:18:32,700 --> 00:18:33,820 +infection site + +246 +00:18:37,560 --> 00:18:43,120 +إذا كان في مكان محدود محدود جغرافيا في الإنسان مثل + +247 +00:18:43,120 --> 00:18:48,200 +حدبة دم التمام تيتانوس في الـ tonsils هذا بسميه + +248 +00:18:48,200 --> 00:18:52,020 +localized أو local infection عكسه systemic أو + +249 +00:18:52,020 --> 00:18:55,900 +generalized و ال generalized بكون ماخد system مثلا + +250 +00:18:55,900 --> 00:18:59,820 +ال lungs أو ال respiratory system ال gastro + +251 +00:18:59,820 --> 00:19:03,320 +intestinal system ممكن يكون في الدم لكن طلعوا + +252 +00:19:06,280 --> 00:19:11,040 +متشابهة مع بعض كيف بنتميزها مع بعض خدوا بالكم لو + +253 +00:19:11,040 --> 00:19:15,780 +انهم او لو في منهم اتنين من التعريفات متطابقين كان + +254 +00:19:15,780 --> 00:19:21,300 +معطنهم تعريفين بس للأسف احنا عدم قدرتنا على + +255 +00:19:21,300 --> 00:19:23,940 +التمييز بين ال terms بتخلينا نستخدمهم + +256 +00:19:23,940 --> 00:19:28,300 +interchangeably يعني اعطيكم مثال تلاتة واربعة + +257 +00:19:28,300 --> 00:19:34,620 +بكتريميا and ستسيميا رأيتموه؟ لو روحنا لطبيبمن + +258 +00:19:34,620 --> 00:19:39,160 +اللي بيعالجه المرضى وعمل ال blood culture عند شخص + +259 +00:19:39,160 --> 00:19:41,400 +معين و طلع عنده بكتيريا، ممكن طبيب يقول عنده + +260 +00:19:41,400 --> 00:19:46,400 +بكتيريميا و آخر يقول عنده A و الاتنان يفهموا بعض، + +261 +00:19:46,400 --> 00:19:49,140 +الاتنان يفهموا بعض أن واحد بيتكلم عن بكتيريا في + +262 +00:19:49,140 --> 00:19:52,860 +الدم و واحد عن بكتيريا في الدم، لكن دايما و أبدا + +263 +00:19:52,860 --> 00:19:57,200 +دقة المصطلح هي اللي خلتنا أنه نعمل مصطلح تاني + +264 +00:19:58,070 --> 00:20:00,610 +فبالتالي ليش احنا .. اذا كان هما اتنين واحد لأ + +265 +00:20:00,610 --> 00:20:04,030 +نلغي واحد نسخ دم؟ و .. لأ .. واقعي انا هما اتنين + +266 +00:20:04,030 --> 00:20:07,470 +مختلفين عن بعضهم البعض نبدأ من الأول توكسيميا + +267 +00:20:07,470 --> 00:20:13,050 +تعبير عن مولود الـ exotoxin في الدم endotoxemia + +268 +00:20:13,050 --> 00:20:16,730 +تعبير عن مولود ميه؟ ال endotoxin في الدم سهل هذا + +269 +00:20:16,730 --> 00:20:21,510 +ال bacterium is an invasion of the bloodstream of + +270 +00:20:21,510 --> 00:20:27,310 +a bio bacteria يعني البكتيريا وصلت للدم لمجرد دمما + +271 +00:20:27,310 --> 00:20:30,670 +اتكلمنا عن تفاصيل، قولنا البكتيريا موردة الآن إيه؟ + +272 +00:20:30,670 --> 00:20:34,430 +أعطيكم مثال، واحد بفرج أسنانه، بتعرفوا فيه بعض + +273 +00:20:34,430 --> 00:20:38,910 +الأحيان لإننا مشاكل في اللثة، ممكن الدم ينزل، الفم + +274 +00:20:38,910 --> 00:20:42,830 +مليء بالبكتيريا، عشر أوص، تسعة، لكل واحد ملي من ال + +275 +00:20:42,830 --> 00:20:47,810 +saliva، أرقام صعبة نعدها، إمكانية إنه تدخل + +276 +00:20:47,810 --> 00:20:51,250 +البكتيريا من مكان الجرح اللي جرح هنا من نظر في + +277 +00:20:51,250 --> 00:20:55,830 +أسناننا، موجودة، لو أنا أخدت blood culture مصادفة + +278 +00:20:56,380 --> 00:20:59,480 +ممكن اعزل البكتيريا اللى طلعت من هون هذه بكتيريا + +279 +00:20:59,480 --> 00:21:03,340 +وسموها ترانزيانت بكتيريا ترانزيانت ايش يعني + +280 +00:21:03,340 --> 00:21:08,360 +ترانزيانت يعني معقدة لإن عددها قليل وغير مباطلين + +281 +00:21:08,360 --> 00:21:13,320 +الجسم بتخلص منها يبقى ده mere presence ايش معنى + +282 +00:21:13,320 --> 00:21:20,420 +مجرد وجود البكتيريا في الذنب بنسميه بكتيريا أما + +283 +00:21:20,420 --> 00:21:27,230 +السبتسيميا فيها مصطلحبكتيريميا زائد تكسيميا التنال + +284 +00:21:27,230 --> 00:21:33,390 +يعني البكتيريا مش بس وصلت الدم بل تكاترت في الدم + +285 +00:21:33,390 --> 00:21:37,490 +ووصل عددها الى ال quorum sensing وبدأت تنتج ايش؟ + +286 +00:21:37,490 --> 00:21:41,330 +التكسيميا اذا سبتسيميا معناها ان البكتيريا مولدة + +287 +00:21:41,330 --> 00:21:46,370 +في الدم بالاضافة الى التكسيميا الفاييميا هي + +288 +00:21:46,370 --> 00:21:50,410 +infection of the blood by a biogenic + +289 +00:21:54,030 --> 00:21:57,490 +Biogenic Infection بيسموها باس بيوزن باس بيوزن باس + +290 +00:21:57,490 --> 00:22:02,850 +بيوزن + +291 +00:22:02,850 --> 00:22:05,650 +باس بيوزن باس بيوزن باس بيوزن باس بيوزن باس بيوزن + +292 +00:22:05,650 --> 00:22:05,830 +باس بيوزن باس بيوزن باس بيوزن باس بيوزن باس بيوزن + +293 +00:22:05,830 --> 00:22:05,930 +باس بيوزن باس بيوزن باس بيوزن باس بيوزن باس بيوزن + +294 +00:22:05,930 --> 00:22:07,130 +باس بيوزن باس بيوزن باس بيوزن باس بيوزن باس بيوزن + +295 +00:22:07,130 --> 00:22:09,110 +باس بيوزن باس بيوزن باس بيوزن باس بيوزن باس بيوزن + +296 +00:22:09,110 --> 00:22:14,590 +باس بيوزن باس بيوزن باساللي هي الـ streptococcus + +297 +00:22:14,590 --> 00:22:18,010 +biodeins اسمها streptococcus biodeins اللي بيسبب + +298 +00:22:18,010 --> 00:22:21,410 +تنسولايتس عشان كده قلت من حبابكم لإنها جزء من + +299 +00:22:21,410 --> 00:22:26,770 +حياتنا الطفولية أنه كثير مرات كده اللي قصبنا بهذا + +300 +00:22:26,770 --> 00:22:27,530 +ال micro + +301 +00:22:39,060 --> 00:22:42,180 +هتبنيها مرحلة ان ده البكتيريا داخلة الدم تيجي ده + +302 +00:22:42,180 --> 00:22:46,540 +مخيار من خارج اما انها تنجح في التكاثر و تصل + +303 +00:22:46,540 --> 00:22:51,640 +لستسيميا هذا خيار او ان هي ان يجب انها تبقى لوتالي + +304 +00:22:51,640 --> 00:22:57,800 +و تبطل لستسيميا اذا في اتلاف بينهم طيب + +305 +00:22:57,800 --> 00:23:01,700 +شايفين الشي على اللوحة انتوا انا مش شايف على + +306 +00:23:01,700 --> 00:23:05,780 +اللوحة اي شي بس لا بس انه تطلع على ال .. ال .. ال + +307 +00:23:11,560 --> 00:23:15,040 +في عندي لو حالة بتقول gram أو some organisms of + +308 +00:23:15,040 --> 00:23:19,300 +medical importance خدوا فالكم أنا أكتر من مرة في + +309 +00:23:19,300 --> 00:23:23,280 +أكتر محاضرة حطيت جداول وفيها أسماء بكتيريا وقرأنا + +310 +00:23:23,280 --> 00:23:27,880 +.. وقرأنا .. وقرأنا أسماء البكتيريا والقراقة هنا + +311 +00:23:27,880 --> 00:23:31,780 +مرة بتكون .. أول مرة بتكون to be familiarized يعني + +312 +00:23:31,780 --> 00:23:35,460 +عشان نخليكم familiar ان الإسم يبطل غريب بس بعد ما + +313 +00:23:35,460 --> 00:23:39,600 +بطل غريب الأصل احنا يعني بالفعل نعرف عنه بعض + +314 +00:23:39,600 --> 00:23:40,780 +المعلومات البسيطة + +315 +00:23:45,480 --> 00:23:51,360 +هنا لجرام نيجاتف كوكساعي زي مين النيسيريا اعتقد في + +316 +00:23:51,360 --> 00:23:55,020 +اسمين في النيسيريا المفروض ان هم صاروا معروفين لكم + +317 +00:23:55,020 --> 00:24:00,780 +نيسيريا جوانورية و النيسيريا مينيديتس او + +318 +00:24:00,780 --> 00:24:05,760 +meningitis في حرف ال D خفيف طبعا ال meningitis هي + +319 +00:24:05,760 --> 00:24:11,130 +المرض بس ال bacterium مينيديتسإذن نيسيريا كومثل + +320 +00:24:11,130 --> 00:24:15,250 +أساسي عن مجموعة بخمة .. مجموعة بخمة .. مجموعة + +321 +00:24:15,250 --> 00:24:19,750 +صغيرة من ال gram negative cocci أو بيسموها gram + +322 +00:24:19,750 --> 00:24:24,870 +negative diplococci على + +323 +00:24:24,870 --> 00:24:28,430 +الجهة التانية ال gram positive cocci facultative + +324 +00:24:28,430 --> 00:24:32,210 +anaerobic ال facultative anaerobic ممثل عنهم + +325 +00:24:32,210 --> 00:24:36,290 +الاستفيلو cocci و الاستريبتو cocci او استريبتو + +326 +00:24:36,290 --> 00:24:40,610 +كوكس واستفيلو كوكسطيب الآن في الستافيلو كوكاس أكتر + +327 +00:24:40,610 --> 00:24:49,670 +اسم بتعرفه؟ خلنا نسمع من اللي قاعدين ورا هناك واش + +328 +00:24:49,670 --> 00:24:55,830 +كمان؟ بس أو yes؟ + +329 +00:25:01,420 --> 00:25:04,700 +أسوأ .. أسوأ حاجة في مرحلة الحفظ إنه يصير ناخده من + +330 +00:25:04,700 --> 00:25:08,240 +.. اسم من هلأ و نحطه على اسم التاني من هلأ، فواضح + +331 +00:25:08,240 --> 00:25:10,420 +أنه فيه بيركب أسماء جديدة من هلأ و نحطله على + +332 +00:25:10,420 --> 00:25:13,140 +الشجرة التانية، ال pneumonia is streptococcus + +333 +00:25:13,140 --> 00:25:15,780 +pneumonia، مش Staphylococcus pneumonia، ال + +334 +00:25:15,780 --> 00:25:19,120 +Staphylococcus epidermis، epidermis عسم المثال + +335 +00:25:19,120 --> 00:25:23,840 +مشهور جدا في Staphylococcinفي ال striptococcus + +336 +00:25:23,840 --> 00:25:26,140 +احنا لسه ذكرنا احد قبل شويه، striptococcus + +337 +00:25:26,140 --> 00:25:29,360 +بيوجينز، و striptococcus pneumonia، و + +338 +00:25:29,360 --> 00:25:31,980 +striptococcus ال pneumonia، و في striptococcus + +339 +00:25:31,980 --> 00:25:36,880 +agalactia، أسماء مشهورة في ال striptococcus تلت + +340 +00:25:36,880 --> 00:25:38,120 +اللي ذكرناهم ال pneumonia + +341 +00:25:41,870 --> 00:25:45,770 +أخرنا اسمها المشهورين Aureas اللي هي الذهبية و + +342 +00:25:45,770 --> 00:25:49,850 +Epidermis واضح من اسمها انها موجودة على الانجل + +343 +00:25:49,850 --> 00:25:56,230 +نيجي لل Spirochetes زي تريبونيميا و درسنا بكتيريا + +344 +00:25:56,230 --> 00:26:00,030 +واحدة منهم على الأقل تريبونيماباليدوم اللي بسبب + +345 +00:26:00,030 --> 00:26:05,470 +مرض السيفلس و ال Borrelia Borrelia برضه من ال + +346 +00:26:05,470 --> 00:26:08,830 +spiral microorganism أخدنا اسم Borrelia + +347 +00:26:08,830 --> 00:26:14,420 +burgdorferiاسمه صعب شوية بوريليا بيرجدرفيري و + +348 +00:26:14,420 --> 00:26:19,660 +بتسبب مرضى بقى اسمه Lyme disease Lyme disease + +349 +00:26:19,660 --> 00:26:28,280 +Leptospira Leptospira من اسمها برضه اشبه بوريليا و + +350 +00:26:28,280 --> 00:26:34,100 +Leptospira it's a spiral microorganism كان جنبهم + +351 +00:26:34,100 --> 00:26:39,400 +Peptococcus + +352 +00:26:39,400 --> 00:26:39,860 +و Peptospira + +353 +00:26:43,380 --> 00:26:46,060 +وكويس الاسمانة بأول مرة عشان يكون تاني مرة يكون + +354 +00:26:46,060 --> 00:26:50,920 +اسمهم مش أول مرة بيبتوكوكس و بيبتوستريبتوكوكس + +355 +00:26:50,920 --> 00:26:55,460 +هدولة التنتين بنسميهم anaerobic cocci anaerobic + +356 +00:26:55,460 --> 00:26:59,880 +cocci يعني من ميزاتهم إنهم بيشبهوا الستريبتوكوكس + +357 +00:26:59,880 --> 00:27:07,860 +لكن لا هوائية إشباعية spiral gram negative زي + +358 +00:27:07,860 --> 00:27:10,720 +نتالاين مشهورين جدا ال campylobutyl + +359 +00:27:13,410 --> 00:27:17,050 +أنا أقولكوا عرفتوها إيه بسبب الهيليكوباكتر؟ و بدنا + +360 +00:27:17,050 --> 00:27:22,310 +إجابة من شخص واحد الهيليكوباكتر + +361 +00:27:22,310 --> 00:27:27,110 +بيادة + +362 +00:27:27,110 --> 00:27:32,070 +الخسارة القرح + +363 +00:27:32,070 --> 00:27:36,890 +الهيليكوباكتر causes peptic ulcer تمام؟ و كنا تسعة + +364 +00:27:36,890 --> 00:27:40,790 +و تسعين تسعة و عشرة من القرح المعدية هي caused by + +365 +00:27:44,680 --> 00:27:48,260 +الـ can be lobacter خدوا المعلومة عنها عشان + +366 +00:27:48,260 --> 00:27:52,760 +ماننسهاش it is number one cause number one cause + +367 +00:27:52,760 --> 00:27:58,600 +of diarrhea in the middle east among children وصلت + +368 +00:27:58,600 --> 00:28:03,640 +المعناه ده لسه هي المسبب رقم واحد للإسهال في منطقة + +369 +00:28:03,640 --> 00:28:07,040 +الشرق الأوسط لدى الأطفال و هي من الميكروبات + +370 +00:28:07,040 --> 00:28:11,530 +المهملة فيهالقطارات ايش يعني مهمة؟ لأ يعني + +371 +00:28:11,530 --> 00:28:15,410 +مابيدورش عليها في حالات ديارية مع انها أصبحت .. + +372 +00:28:15,410 --> 00:28:18,870 +أصبحت يزوء من ال routine في ال school culture في + +373 +00:28:18,870 --> 00:28:25,630 +بعض البلدان السيد ماعرفش الاسم؟ كامبيلو باكتر او + +374 +00:28:25,630 --> 00:28:32,650 +كامبيلو باكتر طيب + +375 +00:28:32,650 --> 00:28:36,110 +بالنسبة للIndospore forming groundpost في بسراي + +376 +00:28:36,110 --> 00:28:37,890 +فيانا اسمين + +377 +00:28:42,820 --> 00:28:48,320 +أيه التلاتة في أسماء من ال Baselas؟ Antracis تمام، + +378 +00:28:48,320 --> 00:28:55,220 +كويس، تمام، هاي .. هاي .. هاي تلاتة و واحد، يعني + +379 +00:28:55,220 --> 00:29:00,180 +هناك الآن عرفنا الأربع أسماء تحت عنوان Gram-Bostef + +380 +00:29:00,180 --> 00:29:05,440 +-Endospore-Forming Baselasطيب فينا gram negative + +381 +00:29:05,440 --> 00:29:11,420 +aerobic basal line زي الجميلة pseudomonas الجنوزة + +382 +00:29:11,420 --> 00:29:17,640 +ليست منها الجميلة لأنها تنتج صبغة زرقاء صفراء + +383 +00:29:17,640 --> 00:29:24,130 +خضراء وما بينهما تمام يعني ممكن تكون الألوانوكمان + +384 +00:29:24,130 --> 00:29:26,950 +شغل تاني سمناها الجميل عشان .. عشان هي إلها رائحة + +385 +00:29:26,950 --> 00:29:31,370 +ذكية، يعني بتنتج fruity .. fruity odor، بسموها .. + +386 +00:29:31,370 --> 00:29:34,690 +إيش يعني fruity؟ يعني فاكهة، عليها الفاكهة، أول ما + +387 +00:29:34,690 --> 00:29:38,490 +نفتح .. نفتح الطبق في ال .. في المختبة، نشتم رائحة + +388 +00:29:38,490 --> 00:29:42,630 +جميلة جدا من الصدومورز، فعلا شكلا و رائحة هي + +389 +00:29:42,630 --> 00:29:47,290 +جميلة، لكن ..من أسوأ أنواع ال infection في + +390 +00:29:47,290 --> 00:29:51,490 +المستشفيات لأنها it causes hard to treat infection + +391 +00:29:51,490 --> 00:29:54,810 +it is antimicrobial resistant او has high + +392 +00:29:54,810 --> 00:29:59,790 +antimicrobial resistance الميكروبيل + +393 +00:29:59,790 --> 00:30:02,650 +التانية في نفس المجموعة ال bordetella و ال + +394 +00:30:02,650 --> 00:30:06,970 +bordetella سمعتوا فيها باسم bordetella pertosis + +395 +00:30:06,970 --> 00:30:12,430 +اللي دراسناها في ال vaccine اللي اسمه DPT طيب مين + +396 +00:30:12,430 --> 00:30:12,830 +مش ذاكر + +397 +00:30:17,540 --> 00:30:21,700 +اللي هي ال Bordetella Pertus تمام؟ ايش اللي عامل + +398 +00:30:21,700 --> 00:30:26,660 +المشترك بين التلاتة هدولة اللي هم ال bordetella و + +399 +00:30:26,660 --> 00:30:30,100 +ال diphtheria و ال tetanus ان التلاتة ال + +400 +00:30:30,100 --> 00:30:34,200 +pathogenesis تبعهم معتمد على ال exotoxin اللي + +401 +00:30:34,200 --> 00:30:37,640 +حولناه toxoid و أعطيناه vaccine و أعطيناه vaccine + +402 +00:30:37,640 --> 00:30:43,020 +طيب في اسم جديد حلو اسمه Francisella مارش معاناه + +403 +00:30:43,020 --> 00:30:43,300 +قبلين + +404 +00:30:52,440 --> 00:30:56,060 +الفرانسيسيلة لها اسم مشهور Francisella Toleransis + +405 +00:30:56,060 --> 00:31:00,100 +وبتعمل + +406 +00:31:00,100 --> 00:31:09,780 +مرض اسمه هم الأرانب rabbits + +407 +00:31:09,780 --> 00:31:13,780 +fever إياكم + +408 +00:31:13,780 --> 00:31:18,400 +عم تظنوا أنه الأرانب الوديعة الجميلة يعني مابتنقلش + +409 +00:31:18,400 --> 00:31:21,240 +أمراضها، قد تنقل أمراض من ضمنها الفرانسيسيلة + +410 +00:31:27,960 --> 00:31:32,800 +الجرامبوسطف بصلاي زي مثلا ال استيريا مرة بقعنا ال + +411 +00:31:32,800 --> 00:31:37,400 +استيريا مرة بقعنا ال استيريا و انا مرة رسمت على + +412 +00:31:37,400 --> 00:31:43,260 +السطورة هنا و قلتلكوا ان في بكتيريا بتدخل من خلية + +413 +00:31:43,260 --> 00:31:48,120 +الى خلية فاكرينها بال أكتين راكتس صواريخ الأكتين + +414 +00:31:48,120 --> 00:31:52,260 +هذه هي ال استيريا طيب احنا بدأنا بنحكي جثث اليوم + +415 +00:31:52,260 --> 00:31:57,330 +كل محاضراتنا جثثبدأ أعطيكوا الآن قصة مرتبطة بال .. + +416 +00:31:57,330 --> 00:32:03,650 +بالاستيريا عشان انعزز ثقافة البحث العلمي وكيفية + +417 +00:32:03,650 --> 00:32:09,950 +تفسير النتائج بعض الأحيان قرأت مقالين متضاربين + +418 +00:32:09,950 --> 00:32:13,510 +مقالين أقصر الأبحاث بحتان يعني واحد بيقول إن ال + +419 +00:32:13,510 --> 00:32:16,810 +استيريا monocytogen بتموت بالبسترة فاكرين البسترة + +420 +00:32:16,810 --> 00:32:21,930 +72 درجة مئوية لمدت 15 ثانية بسترة + +421 +00:32:24,120 --> 00:32:28,960 +الان الباحث بيقول انه بتموت بال pasteurization ال + +422 +00:32:28,960 --> 00:32:32,260 +conclusion تبعته و التاني بيقول انه لأ بتموتش بال + +423 +00:32:32,260 --> 00:32:38,600 +pasteurization قرأت الباحثين مرارا و متكرارا طريقة + +424 +00:32:38,600 --> 00:32:43,440 +تقريبا نفس الطريقة في الآخر انتبهت لنقطة معينةإن + +425 +00:32:43,440 --> 00:32:46,920 +الباحث الأول بيقول انا أخدت العينة اللى اشتغلت + +426 +00:32:46,920 --> 00:32:49,920 +عليها عامة ال testing تبعي عليها أخدتها من بقرة + +427 +00:32:49,920 --> 00:32:54,580 +مصعبة بالليستيريا انا أخدت حليب فيه جواته ليستيريا + +428 +00:32:54,580 --> 00:32:57,940 +التانى لأ جاب ليستيريا اللى زلعها هو فى التلاجة + +429 +00:32:57,940 --> 00:33:01,120 +وحطها فى الحليب فإيش الفرق بين اتنين ان هذا من + +430 +00:33:01,120 --> 00:33:07,120 +original source وهذا من طبق طبعا لما .. لما .. لما + +431 +00:33:07,120 --> 00:33:13,030 +اتبعت الموضوع هذا بدأت الخيوط تتبدلأول حاجة من هنا + +432 +00:33:13,030 --> 00:33:17,390 +ناخد بعض العبار من الموضوع ان هاي فيها تتضارب في + +433 +00:33:17,390 --> 00:33:21,910 +نتائج الأبحاث انتوا في الكتب .. في الكتب غالبا + +434 +00:33:21,910 --> 00:33:27,790 +تجيكوا المعلومة المصفاة المنقاة بدون شواقب تمام؟ و + +435 +00:33:27,790 --> 00:33:30,790 +بتهيألي لإن احنا لو .. لو كل مرة نقولكوا في دراسة + +436 +00:33:30,790 --> 00:33:32,930 +بتقول كده و في دراسة بتقول كده ولا حد حافظ على أي + +437 +00:33:32,930 --> 00:33:36,550 +معلومة فبنتفر في ال textbook نعطيكوا المعلومات + +438 +00:33:36,550 --> 00:33:41,150 +اللي شبه المتفق عليهابينما في واقع البحث العلمي + +439 +00:33:41,150 --> 00:33:45,370 +حللي كتير معلومات في ال microbiology مختلف عليها + +440 +00:33:45,370 --> 00:33:49,150 +قد يكون الاختلاف حقيقي و قد يكون الاختلاف غير + +441 +00:33:49,150 --> 00:33:51,770 +حقيقي زي المثال اللي انا ذاكرته قبل شوية اختلاف + +442 +00:33:51,770 --> 00:33:56,190 +غير حقيقي هذا اختلاف لأن الطريقة المتبعة في الشغل + +443 +00:33:56,190 --> 00:34:01,910 +كانت مختلفة و قدت إلى نتاج مختلفة ال science يعرف + +444 +00:34:01,910 --> 00:34:05,410 +على انه a field of study that deals with + +445 +00:34:08,150 --> 00:34:11,330 +interpretation of reproducible كلمة reproducible + +446 +00:34:11,330 --> 00:34:15,030 +هي دي key word و حطتها مليون خط reproducible facts + +447 +00:34:15,030 --> 00:34:20,370 +البحث أو ال experiment لازم تطلع نفس النتيجة + +448 +00:34:20,370 --> 00:34:23,750 +reproducible يعني كل مرة بنعيدها بتتعطيل نفسها + +449 +00:34:23,750 --> 00:34:27,190 +النتيجة يده بتبقى على نفس الطريقة لكن الطريقة هى + +450 +00:34:27,190 --> 00:34:30,350 +اللي حصل فيها الأن somewhere اختلف فبندرس الدرس + +451 +00:34:30,350 --> 00:34:35,970 +التاني ان كيف نفسر الاختلافاتمش دايما نقول والله + +452 +00:34:35,970 --> 00:34:41,070 +واحد منهم ايه، غشاش، على طول نفتي أو نحكم بدون + +453 +00:34:41,070 --> 00:34:47,170 +مرجعية علمية، اقع جيدا، حاول تطلع على اي فروقات، + +454 +00:34:47,170 --> 00:34:51,210 +شوف ال temperatureاحنا في الأبحاث لما بنشر الأبحاث + +455 +00:34:51,210 --> 00:34:54,330 +بنحط بين جثين الشركة اللى حطينا فيها ال incubator + +456 +00:34:54,330 --> 00:34:58,290 +ال incubator اللى مختلف من الشركة لأ ممكن يكون دقة + +457 +00:34:58,290 --> 00:35:01,910 +ال incubator هى اللى اختلفت لما انا بقول عزالة + +458 +00:35:01,910 --> 00:35:04,210 +سلمونيلا في غزة على سبيل المثال على سبيل الممارسة + +459 +00:35:04,210 --> 00:35:07,750 +الحقيقية 5% و سمين اللى يقولي قول عزالة عشرت في 10 + +460 +00:35:07,750 --> 00:35:11,650 +% عارفين ال incubation temperature لو ارتفعت نص + +461 +00:35:11,650 --> 00:35:14,410 +درجة قد تؤثر على ال frequency و ال isolation؟ + +462 +00:35:15,230 --> 00:35:18,130 +فبالتالي اللي انا بدى أقوله من .. من .. من ال .. + +463 +00:35:18,130 --> 00:35:20,450 +ال .. ال .. الجزئي اللي .. او ال مثال اللي حكيته + +464 +00:35:20,450 --> 00:35:25,490 +انه بيكون المقدرة على تفسير الاختلافات ما بين + +465 +00:35:25,490 --> 00:35:29,150 +الحقائق والمعلومات المتوفرة لدينا في السوق مثال + +466 +00:35:29,150 --> 00:35:35,670 +تاني كنا بنعمل حقيقة معروفة اسمها urinary tract + +467 +00:35:35,670 --> 00:35:40,670 +infection it is more common in females than males + +468 +00:35:40,670 --> 00:35:49,770 +بيبقى واحد بيفسرممكن يفسر مليون تفسير، منطقي، + +469 +00:35:49,770 --> 00:35:53,290 +تفسير منطقية، لكن مش بالضرورة يكون التفسير إيه + +470 +00:35:53,290 --> 00:35:58,150 +صحيح، وأعطيكم مثال على التفسير المنطقي والتفسير + +471 +00:35:58,150 --> 00:36:02,430 +الصحيح، ثلاث فلحين مروحين من الشغل، تبقى هم كل + +472 +00:36:02,430 --> 00:36:05,590 +واحد حاط وطريقه على كدفه، تعبوا في الطريق، اللي + +473 +00:36:05,590 --> 00:36:10,350 +أجلبوا محطة انتظار البساطي على تحتها، فجرا واحد + +474 +00:36:10,350 --> 00:36:14,900 +مات إيده هكي، قال إيه؟ الدنيا بتمطرو سأل سؤال قال + +475 +00:36:14,900 --> 00:36:18,620 +what causes the rain؟ ما الذي يسبب المطر؟ واحد + +476 +00:36:18,620 --> 00:36:25,220 +منهم قال في في السماء سناديق و لما الإله بده ينزل + +477 +00:36:25,220 --> 00:36:31,760 +المطر بيأمر الريح تجليه السناديق، التاني قال لأ مش + +478 +00:36:31,760 --> 00:36:37,550 +سناديق فيه سفنجالبخار بيطلع من الأرض .. من كوكب + +479 +00:36:37,550 --> 00:36:41,390 +الأرض و الاسفنج بينطصه و لما الإله بده ينزل المطر + +480 +00:36:41,390 --> 00:36:46,190 +بيبقى تنريح بيعصر الاسفنج بينزل المطر التالت قال + +481 +00:36:46,190 --> 00:36:54,010 +لأ الماء بتبخر و بيطلع فوق بيلاقي هواء بارد بتكثف + +482 +00:36:54,010 --> 00:36:57,750 +البرمجة التي عرفناه الآن السؤال اللي انا هاطلعه + +483 +00:36:57,750 --> 00:37:02,410 +عليكم مين منهم فسر موضوع المطر؟ التالت .. التالت + +484 +00:37:02,410 --> 00:37:02,850 +فسر + +485 +00:37:23,040 --> 00:37:27,740 +الثلاثة حاولوا وفسروا نزول المطر لكن السؤال which + +486 +00:37:27,740 --> 00:37:32,230 +of that explanation was testedالاخير اللي هو + +487 +00:37:32,230 --> 00:37:34,830 +اعملولي testing على البراد الشاي لما حطي علي + +488 +00:37:34,830 --> 00:37:38,750 +الجزاز و اتكلمت عليه و نزل مطر، مضطر لأ؟ فاللي + +489 +00:37:38,750 --> 00:37:42,470 +بتقوله انا انه في ال science we accept logical + +490 +00:37:42,470 --> 00:37:48,170 +logical explanation، تفسيرات منطقيةاحنا خرجنا عن + +491 +00:37:48,170 --> 00:37:52,650 +الموضوع خالص لكن لإنه أنا معني و لإنه احنا في علم + +492 +00:37:52,650 --> 00:37:55,110 +اسمه علم ال micro body اللي بندرسه كتير من + +493 +00:37:55,110 --> 00:37:58,230 +المعلومات اللي هنجيها بعد ما نخلص هنجيها في بعض + +494 +00:37:58,230 --> 00:38:00,730 +الأحيان المتلقبة فبنكون عندى المقدرة و بداية + +495 +00:38:00,730 --> 00:38:05,030 +المقدرة إنه أتقبل هذا الاقتلاق بسبب إيش قلناها؟ + +496 +00:38:05,360 --> 00:38:08,760 +بسبب انه ممكن يكون different methodologies او + +497 +00:38:08,760 --> 00:38:11,000 +different sample sites انا اشتغلت على تلاتمية و + +498 +00:38:11,000 --> 00:38:14,600 +اشتغل على خمسمية انا اشتغلت في منطقة جغرافية فسيطة + +499 +00:38:14,600 --> 00:38:18,000 +و اشتغل في منطقة جغرافية واسعة المنطقة الجغرافية + +500 +00:38:18,000 --> 00:38:23,980 +مصر غير عن غزرالأردن غير عن سوريا، الامريكا غير، + +501 +00:38:23,980 --> 00:38:27,920 +الديانة بتفرج، ال .. ال .. ال .. نعم بتأثر على + +502 +00:38:27,920 --> 00:38:31,100 +نتاجي، احنا بلا نصب .. بلا صعب بيه الديدار اللي في + +503 +00:38:31,100 --> 00:38:33,960 +التقرع عن طريق الخنزير لإن احنا مسلمين، فلو عملنا + +504 +00:38:33,960 --> 00:38:37,380 +في .. في community في أمريكا مسلمة، ولا لإن عندهم + +505 +00:38:37,380 --> 00:38:40,280 +zero دودة شريطية ونقول إن أمريكا مفيش فيها دودة + +506 +00:38:40,280 --> 00:38:42,440 +شريطية، ما احنا .. احنا .. لإنه احنا عملنا + +507 +00:38:42,440 --> 00:38:46,660 +community ..مسلمة، فالقصد من الكلام أنه بيكون عن + +508 +00:38:46,660 --> 00:38:51,340 +المقدر على أنه إيه أقوم ببعض التفسيرات للاختلافات، + +509 +00:38:51,340 --> 00:38:54,420 +الجزء الأخير من الجدول هو عبارة عن ال + +510 +00:38:54,420 --> 00:38:57,700 +enterobacteriasi زي الإشريشيا، السلمونيلا، + +511 +00:38:57,700 --> 00:39:02,040 +الشيجيلا، اليارسينيا، مارعكوا اليارسينيا قبل هيك؟ + +512 +00:39:02,040 --> 00:39:02,720 +يارسينيا + +513 +00:39:07,770 --> 00:39:10,790 +في ال enterobacter و ال protease و ال ceratia و ال + +514 +00:39:10,790 --> 00:39:13,290 +edwards اللي لكل مدولة بنعتبرهم احنا + +515 +00:39:13,290 --> 00:39:18,490 +enterobacterias فينا ميكروبات اخرى زي ال vibrio و + +516 +00:39:18,490 --> 00:39:21,190 +ال hemophilus ال vibrio طبعا مشهورة ال vibrioplera + +517 +00:39:21,190 --> 00:39:26,410 +ال hemophilus influenza الباستوريلا اللى كانت اللى + +518 +00:39:26,410 --> 00:39:30,870 +جرسينا فيه يوم من ايام تسمى pastorella Legionella + +519 +00:39:30,870 --> 00:39:35,010 +حلو هذا ال Legionella اسم برضه هيمور معانا ان شاء + +520 +00:39:35,010 --> 00:39:38,520 +الله بعدينمن الميكروبات الأولوية التانية اللي هي + +521 +00:39:38,520 --> 00:39:42,720 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +522 +00:39:42,720 --> 00:39:43,840 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +523 +00:39:43,840 --> 00:39:43,880 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +524 +00:39:43,880 --> 00:39:48,760 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +525 +00:39:48,760 --> 00:39:48,920 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +526 +00:39:48,920 --> 00:39:49,460 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +527 +00:39:49,460 --> 00:39:49,520 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +528 +00:39:49,520 --> 00:39:51,720 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +529 +00:39:51,720 --> 00:39:51,780 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +530 +00:39:51,780 --> 00:39:51,840 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +531 +00:39:51,840 --> 00:39:51,900 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +532 +00:39:51,900 --> 00:39:54,180 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +533 +00:39:54,180 --> 00:39:57,800 +ال .. + +534 +00:39:57,800 --> 00:39:59,380 +ال + +535 +00:40:06,970 --> 00:40:11,570 +و ال Bartonella و ال Clamydia و ال Rickettsia و ال + +536 +00:40:11,570 --> 00:40:16,550 +Mycoplasma في طبعا بعض الميكروبات الأخرى اللي احنا + +537 +00:40:16,550 --> 00:40:19,830 +ما ذكرناها غالبا هي أقل أهمية من الميكروبات + +538 +00:40:19,830 --> 00:40:20,250 +التالية + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/gejJKbVhKOc_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/gejJKbVhKOc_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..6a569fd13ae11081bb47690ddf4fbea9acdad958 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/gejJKbVhKOc_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 4114, 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Leptospira it's a spiral microorganism كان جنبهم Peptococcus و Peptospira", "tokens": [32277, 2304, 3224, 20328, 3615, 3555, 13412, 2407, 10632, 4724, 13063, 26895, 25528, 4724, 13546, 7435, 3215, 28480, 13546, 1829, 4032, 39894, 35457, 3555, 3714, 43042, 7578, 4724, 4587, 7578, 24525, 2304, 3224, 12687, 1398, 4752, 12687, 1398, 4752, 441, 5250, 2763, 4271, 441, 5250, 2763, 4271, 9154, 24525, 2304, 11296, 4724, 43042, 3224, 1975, 8592, 3555, 3224, 4724, 13063, 26895, 25528, 4032, 441, 5250, 2763, 4271, 309, 311, 257, 25165, 49129, 1434, 25961, 10874, 1863, 3555, 16095, 430, 5250, 905, 905, 1149, 4032, 430, 5250, 2763, 4271], "avg_logprob": -0.33760532636320995, "compression_ratio": 1.5842696629213484, "no_speech_prob": 0.0, "words": [{"start": 1570.72, "end": 1571.06, "word": "اسمه", "probability": 0.9205729166666666}, {"start": 1571.06, "end": 1571.32, "word": " صعب", "probability": 0.96044921875}, {"start": 1571.32, "end": 1571.74, "word": " شوية", "probability": 0.9072265625}, 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+00:00:31,020 --> 00:00:36,900 +كنا بدأنا الحديث المرة الماضية على جزئية متعلقة + +5 +00:00:36,900 --> 00:00:39,960 +بالـ artificial cultivation of microorganisms وكنا + +6 +00:00:39,960 --> 00:00:43,260 +أن كل اللي درسناه من هذا الـ chapter أساسا موظف + +7 +00:00:43,260 --> 00:00:47,680 +لخدمة اللي هو هذا الجزء من المحاضرة إنه كيف نمي ال + +8 +00:00:47,680 --> 00:00:53,560 +microorganism وتحدثنا عن أنواع و تصنيفات لـ Media + +9 +00:00:53,560 --> 00:00:56,480 +مثل الـ Minimal Essential Medium مثل الـ Enrichment + +10 +00:00:56,480 --> 00:01:00,640 +Media مثل الـ Selective و الـ Differential Media + +11 +00:01:01,460 --> 00:01:05,200 +واللوحة أمامكم بتقول أنه فيه ثلاثة أصناف من الـ media + +12 +00:01:05,200 --> 00:01:08,200 +based on physical condition، يا إما solid، يا إما + +13 +00:01:08,200 --> 00:01:13,280 +liquid، يا إما semi-solid، والثلاثة أشكال أكيد مدام + +14 +00:01:13,280 --> 00:01:17,120 +موجودين يبقى لهم استخداماتهم، أحد الأشياء اللي + +15 +00:01:17,120 --> 00:01:22,160 +لازم نؤكد عليها اللي هو الـ solid media بتلاحظوا + +16 +00:01:22,160 --> 00:01:25,720 +الآن اللي هو اللي قدامكم فيه طبقين، الطبق الأول كل + +17 +00:01:25,720 --> 00:01:29,280 +المستعمرات فيه لون أبيض، وأصل الوسط لونه شفاف + +18 +00:01:29,280 --> 00:01:33,780 +الوسط، والثانية فيه أصفر وفيه أحمر فيه أكثر من لون + +19 +00:01:33,780 --> 00:01:37,920 +فيه الـ .. الـ .. الوسط اللي بيحتوي على أكثر من لون + +20 +00:01:37,920 --> 00:01:42,360 +ماذا يعني ذلك؟ أنه فيه أكثر من نوع بكتيريا يبقى + +21 +00:01:42,360 --> 00:01:46,180 +البكتيريا اللي زرعت على هذا الطبق لم تكن pure، ماذا + +22 +00:01:46,180 --> 00:01:50,890 +نقصد بـ pure؟ معناها نقية، نقية يعني مستعمرة لا تحتوي + +23 +00:01:50,890 --> 00:01:55,950 +إلا على صنف واحد من البكتيريا، غالبا احنا كيف في + +24 +00:01:55,950 --> 00:01:58,630 +المعمل بنحصل على صنف واحد من البكتيريا أو pure + +25 +00:01:58,630 --> 00:02:06,260 +culture؟ بنفترض جدا أن كل مستعمرة نشأت من خلية + +26 +00:02:06,260 --> 00:02:10,240 +واحدة يبقى كل مستعمرة أمامك هنا على السبورة أو على + +27 +00:02:10,240 --> 00:02:13,380 +اللوحة تمام؟ مثل مثلا اللي .. اللي .. اللي .. اللي + +28 +00:02:13,380 --> 00:02:17,840 +بنشير لها بالسهم هذه نشأت من إيه؟ هذا افتراض جدلي + +29 +00:02:17,840 --> 00:02:22,660 +صحيح بنسبة كبيرة جدا لكن مش absolute لكن احنا + +30 +00:02:22,660 --> 00:02:26,080 +ماشين عليه بمعنى أنه لأن ممكن اثنين بكتيريا + +31 +00:02:26,080 --> 00:02:32,130 +يكونوا مستعمرة واحدة، وبتالي هو افتراض جدلي، لكن هذه + +32 +00:02:32,130 --> 00:02:35,430 +أحد مميزات الـ solid media، ايش مميزات الـ solid media + +33 +00:02:35,430 --> 00:02:39,810 +أنه الـ growth تبع البكتيريا بيكون بشكل الـ colonies + +34 +00:02:39,810 --> 00:02:43,530 +طب ما فائدة الـ colonies؟ واحد متأكد من الـ purity + +35 +00:02:43,530 --> 00:02:49,230 +of the culture، لقم اثنين الـ identity of the + +36 +00:02:49,230 --> 00:02:53,070 +microorganism could be identified using cultural + +37 +00:02:53,070 --> 00:02:56,860 +characteristics، ليش يعني؟ يعني الصفات المزرعية اللي + +38 +00:02:56,860 --> 00:03:01,200 +هي شكل المستعمرة، ارتفاعها، لونها، حجمها، الـ + +39 +00:03:01,200 --> 00:03:05,600 +texture تبعتها، القوام تبعتها، هل هو لزج، هل هو + +40 +00:03:05,600 --> 00:03:11,020 +watery، هل هو dry، هل هو wet، هل هي حمراء، خضراء، + +41 +00:03:11,020 --> 00:03:15,440 +بيضاء، مرتفعة، إلها قبة، هذه يسميها أشكال + +42 +00:03:15,440 --> 00:03:20,830 +المستعمرة، كل نوع معين من البكتيريا إيه له شكل محدد + +43 +00:03:20,830 --> 00:03:24,650 +من إيه؟ من المستعمرات، فإذا رأيت الشكل المحدد + +44 +00:03:24,650 --> 00:03:29,230 +تعرفتِ على مين؟ على النوع، وبتلاقي ميزة للـ solid + +45 +00:03:29,230 --> 00:03:32,810 +media أن النمو بيكون فيها بشكل مستعمرات بينما الـ + +46 +00:03:32,810 --> 00:03:36,570 +liquid media النمو فيها بيكون بشكل turbidity، ايش + +47 +00:03:36,570 --> 00:03:40,050 +يعني turbidity؟ يعني عكرة بمعنى إنه من طالع + +48 +00:03:40,050 --> 00:03:44,670 +الأنبوبة بنشوف إنه إذا فيش growth، no turbidity، + +49 +00:03:44,670 --> 00:03:45,930 +في growth، في turbidity + +50 +00:03:48,650 --> 00:03:52,250 +هي عكرة بتختلف عن عكرة أخرى، التربيد كلها + +51 +00:03:52,250 --> 00:03:57,270 +تتشابه، إذن هي أول advantage للـ solid media عالمين + +52 +00:03:57,270 --> 00:04:01,130 +على الـ liquid media إنه النمو بيكون شكل مستعمرات + +53 +00:04:01,130 --> 00:04:04,690 +طب ما هي الـ advantage تبع الـ liquid media على الـ + +54 +00:04:04,690 --> 00:04:08,850 +solid media؟ أحد أهم الـ advantages اللي هو سرعة + +55 +00:04:08,850 --> 00:04:13,680 +النمو، ليش سرعة النمو؟ البكتيريا في الـ liquid media + +56 +00:04:13,680 --> 00:04:16,780 +is in direct contact .. in direct contact يعني على + +57 +00:04:16,780 --> 00:04:20,080 +اتصال مباشر مع مين؟ مع الـ nutrient .. بينما في الـ + +58 +00:04:20,080 --> 00:04:23,400 +solid media .. طلع الـ solid media .. هل الـ + +59 +00:04:23,400 --> 00:04:26,640 +nutrient اللي في المنطقة هذه available للمستعمرة + +60 +00:04:26,640 --> 00:04:30,760 +هذه؟ مش available .. لكن لو كانت في liquid .. ايش + +61 +00:04:30,760 --> 00:04:35,180 +بيعمل الـ liquid؟ لو البكتيريا .. لو البكتيريا + +62 +00:04:35,180 --> 00:04:40,300 +متحركة، هتنتقل من مكان إلى آخر، طب لو غير متحركة، + +63 +00:04:40,300 --> 00:04:43,660 +جزيئات السائل لها حركة، بيسموها .. بيسموها + +64 +00:04:43,660 --> 00:04:47,560 +Brownian Movement of Water Molecules، برضه هتنقل + +65 +00:04:47,560 --> 00:04:52,620 +البكتيريا من مكان إلى مكان آخر، إذا في الـ .. في الـ + +66 +00:04:52,620 --> 00:04:55,520 +liquid media، إذا أنا بدي أنمي البكتيريا بشكل + +67 +00:04:55,520 --> 00:05:00,080 +سريع، بنميها في liquid media، إذا بدي أحصل على + +68 +00:05:00,080 --> 00:05:04,200 +مستعمرات عشان أحصل على pure culture أو أحصل على + +69 +00:05:04,200 --> 00:05:08,280 +identification للبكتيريا بنميها على solid medium + +70 +00:05:08,280 --> 00:05:14,720 +قدامكم لوحة بتتكلم عن الـ semi-solid medium، الـ + +71 +00:05:14,720 --> 00:05:19,920 +semi-solid غرض أساسي إلها to detect the motility + +72 +00:05:19,920 --> 00:05:23,920 +of bacteria، فاكرين لما تكلمنا عن الـ flagella وأن + +73 +00:05:23,920 --> 00:05:28,840 +البكتيريا بتتحرك بالـ flagella، سباحة ولا طيران ولا + +74 +00:05:28,840 --> 00:05:34,640 +ماشي، تسبح، والسباحة بتكون في ايه؟ في سائل، أنا لسه + +75 +00:05:34,640 --> 00:05:39,520 +قبل دقيقة بقول إنه البكتيريا الغير متحركة قادرة + +76 +00:05:39,520 --> 00:05:43,120 +على الانتقال في السائل من مكان إلى مكان بذات حركة + +77 +00:05:43,120 --> 00:05:46,340 +جزيئات في السائل، إذا كيف بتنتقل البكتيريا + +78 +00:05:46,340 --> 00:05:50,840 +متحركة أو غير متحركة؟ ع الصلب بتمشيش، في الـ liquid + +79 +00:05:50,840 --> 00:05:55,160 +هتختلط الحابل منها بنظرا ليه حركة وجهة السائل + +80 +00:05:55,160 --> 00:05:58,620 +فصممنا وسط ما بينهما اللي هو الـ semi-solid media + +81 +00:05:58,620 --> 00:06:01,740 +طلعوا على الأنبوبة ها دي اللي هي أمامنا الآن اللي + +82 +00:06:01,740 --> 00:06:06,300 +باللون الأحمر، ها دي زرعنا البكتيريا بشكل طعنة، step بعد + +83 +00:06:06,300 --> 00:06:09,160 +ما نمت البكتيريا، نمت في نفس مكان الطعنة، دلوقتي على + +84 +00:06:09,160 --> 00:06:13,340 +أنه لم تتحرك عن نطاق خط الطعنة، بينما في الأنبوبة + +85 +00:06:13,340 --> 00:06:18,590 +الثانية، طب عاملناها بنفس الكيفية لكن لاحظنا الآن + +86 +00:06:18,590 --> 00:06:23,950 +انتشار للبكتيريا، دلوقتي البكتيريا متحركة وهذا ما + +87 +00:06:23,950 --> 00:06:27,130 +نقوم به في فحص الـ motility في المعمل في الـ semi + +88 +00:06:27,130 --> 00:06:32,530 +-solid، إذا الـ liquid و الـ solid مهمين وكمان الـ + +89 +00:06:32,530 --> 00:06:38,190 +semi-solid أيضا له أهمية خاصة، لماذا نقوم بزراعة + +90 +00:06:38,190 --> 00:06:42,090 +البكتيريا في المعمل؟ كمان مرة سؤال لازم نجاوب عليه + +91 +00:06:42,090 --> 00:06:45,950 +ونكون فاهمين إن احنا ما زرعناهاش عشان نتسلى ونشوف + +92 +00:06:45,950 --> 00:06:50,550 +ألوانها الجذابة، لأ وإنما لأغراض محددة، واحد + +93 +00:06:50,550 --> 00:06:57,910 +isolation and identification، لازم نعرف البكتيريا + +94 +00:06:57,910 --> 00:07:03,810 +تخيلوا معي الوضع التالي، تسمعوا بمرض التهاب السحايا + +95 +00:07:03,810 --> 00:07:09,270 +اللي هو الـ meningites، من الذي يسبب الـ meningitis؟ + +96 +00:07:09,270 --> 00:07:15,110 +طلعوا الأسباب في الأربع، بكتيريا، بكتيريا، + +97 +00:07:15,110 --> 00:07:19,990 +viruses، parasites و fungi، طالما بنقول نسينا من + +98 +00:07:19,990 --> 00:07:23,490 +الثلاثة ونأخذنا في واحد، بكتيريا، هل البكتيريا نوع + +99 +00:07:23,490 --> 00:07:26,770 +واحد؟ Staphylococcus aureus، Pseudomonas + +100 +00:07:26,770 --> 00:07:29,230 +aeruginosa، Escherichia coli، Hemophilus + +101 +00:07:29,230 --> 00:07:32,050 +influenza، Neisseria meningitidis، Streptococcus + +102 +00:07:32,050 --> 00:07:34,770 +pneumoniae، مجموعة ضخمة من البكتيريا كلها بتسبب مرض + +103 +00:07:34,770 --> 00:07:40,360 +واحد اسمه إيه؟ meningitis، والطبيب بيشخص مرض اسمه + +104 +00:07:40,360 --> 00:07:45,520 +meningitis بس ما بيشخصش المسبب تبع الـ meningitis، أنا + +105 +00:07:45,520 --> 00:07:47,880 +تكلمت من الأسماء اللي ذكرتها في منها gram + +106 +00:07:47,880 --> 00:07:51,340 +negative ومنها gram positive، حتى العلاج تبعها هيبقى + +107 +00:07:51,340 --> 00:07:54,080 +يختلف، الـ antibiotic تبعها هيبقى يختلف، وبالتالي + +108 +00:07:54,080 --> 00:07:58,560 +عملية عزل الـ micro والتأكد من هويته مهم جدا في + +109 +00:07:58,560 --> 00:08:03,370 +اختيار المريض، بالمناسبة ده واحد، رقم اثنين + +110 +00:08:03,370 --> 00:08:07,210 +for epidemiological، وهدي نقطة سأكررها مرارا أو + +111 +00:08:07,210 --> 00:08:12,150 +تكرارا في الفصل هذا والفصل القادم، For + +112 +00:08:12,150 --> 00:08:17,570 +epidemiological reasons، الآن لو سألنا سؤال عام، ما + +113 +00:08:17,570 --> 00:08:25,410 +هو أكثر مسبب لتسمم الغذاء في فلسطين؟ ليه أحد + +114 +00:08:25,410 --> 00:08:30,490 +يعلم؟ عارفين ليش؟ لأنه لا يتم التحقق ولا يتم عزل + +115 +00:08:30,490 --> 00:08:33,870 +وتعريف الميكروبات المسببة لمن؟ للسموم الغذائية، مش + +116 +00:08:33,870 --> 00:08:36,870 +هاعرف الإجابة على كثير من الأسئلة المتعلقة + +117 +00:08:36,870 --> 00:08:40,970 +بالوبائيات، طالما ما أنا ما عنديش معرفة بالتعرف على + +118 +00:08:40,970 --> 00:08:45,690 +الميكروبات المسببة لها الأكثر وانتشارها، مين أكثر + +119 +00:08:45,690 --> 00:08:51,700 +انتشارا في السلمونيلا؟ خانيونس ولا غزة؟ عندي معرفة + +120 +00:08:51,700 --> 00:08:56,700 +خانيونس تمام؟ أكثر من غزة، ليش؟ لأنه بيتم عزل + +121 +00:08:56,700 --> 00:08:59,220 +الميكروب من الستول تبع الأطفال اللي عندهم ديارية + +122 +00:08:59,220 --> 00:09:01,580 +أو الأشخاص اللي عندهم ديارية وبالتالي في عندي + +123 +00:09:01,580 --> 00:09:05,320 +إحصائية بتقول إن نسبة العزل للسلمونيلا في خانيونس + +124 +00:09:05,320 --> 00:09:09,300 +أعلى منها في مدينة غزة، يبقى لما يكون عندي معلومات + +125 +00:09:09,300 --> 00:09:15,660 +من هذا النوع أستخدمها في التخطيط، وبسأل سؤال لماذا + +126 +00:09:15,660 --> 00:09:20,120 +في خان يونس مش في غزة؟ لأن جزء كبير من مناطق خان يونس + +127 +00:09:20,120 --> 00:09:23,160 +غير موصولين بنظام المجاري المبلغ + +128 +00:09:26,450 --> 00:09:30,530 +يصل أكبر عدد ممكن من المنازل اللي في خان يونس إن أنا + +129 +00:09:30,530 --> 00:09:34,570 +متأكد إن نسبة الطفيليات ونسبة السالمونيلا هتقل في + +130 +00:09:34,570 --> 00:09:39,290 +تلك المنطقة، إذا العزل وتعريف الميكروبات مهم جدًا + +131 +00:09:39,290 --> 00:09:43,090 +لأغراض متنوعة وعديدة منها الـdiagnosis منها الـ + +132 +00:09:43,090 --> 00:09:45,790 +treatment منها الـepidemiological investigation + +133 +00:09:45,790 --> 00:09:50,690 +purification أنا + +134 +00:09:50,690 --> 00:09:55,310 +عندي خلطة من الميكروبات، بدي أعزل واحد منهم، بدي + +135 +00:09:55,310 --> 00:09:59,250 +أعزل واحد فقط منهم، يبقى بدي أزرع، عشان أعزله لازم + +136 +00:09:59,250 --> 00:10:02,450 +أزرع، لأن في مستعمرة حمراء ومستعمرة زرقاء ومستعمرة + +137 +00:10:02,450 --> 00:10:06,650 +مختلطة، بسرهم مع بعض، طب ليش بتعمل الـpurification؟ + +138 +00:10:06,650 --> 00:10:10,010 +تخيلوا أنا بدي أنمي بكتيريا عشان أعمل vaccine، + +139 +00:10:10,010 --> 00:10:13,050 +vaccine ضد الـStaphylococcus aureus أو الـ + +140 +00:10:13,050 --> 00:10:16,360 +Staphylococcus pneumonia، بنفع يكون معاه ميكروبات + +141 +00:10:16,360 --> 00:10:21,260 +أخرى؟ أنا بنتحقن المريض بهذا أو الشخص بهذا الـ .. + +142 +00:10:21,260 --> 00:10:22,420 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +143 +00:10:22,420 --> 00:10:22,720 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +144 +00:10:22,720 --> 00:10:23,120 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +145 +00:10:23,120 --> 00:10:23,460 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +146 +00:10:23,460 --> 00:10:24,200 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +147 +00:10:24,200 --> 00:10:24,560 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +148 +00:10:24,560 --> 00:10:24,620 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +149 +00:10:24,620 --> 00:10:27,080 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +150 +00:10:27,080 --> 00:10:35,720 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +151 +00:10:35,720 --> 00:10:36,580 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +152 +00:10:36,580 --> 00:10:41,180 +.. الـ .. الـ + +153 +00:10:48,890 --> 00:10:53,370 +تخيل لو أنا أعطيتك يوم في قنينة وقلت لك إيه اعمل + +154 +00:10:53,370 --> 00:10:58,490 +الاندول والميثيلريد والرُّكوسبروسكوار والسيتري، إيش + +155 +00:10:58,490 --> 00:11:01,910 +النتائج هيطلع؟ positive و positive و positive و + +156 +00:11:01,910 --> 00:11:05,890 +positive فهيطلع النتيجة عند اللي قدامي أربعة + +157 +00:11:05,890 --> 00:11:08,690 +positive لأن أنا بتكلم عن مين؟ عن two organism أنا + +158 +00:11:08,690 --> 00:11:12,910 +أفكرهم one organism، فأنت بدأت تطلع على الريداول، + +159 +00:11:12,910 --> 00:11:16,290 +تقارن مين من الميكروبات اللي بترك هذه الصفات، + +160 +00:11:16,290 --> 00:11:19,410 +أربعة بوستيف، مش هتلاقي ولا ميكروب، هتقولي هي أنا + +161 +00:11:19,410 --> 00:11:24,430 +اتشافت ميكروب، ماشي دي، إذا أحد أهم الأخطاء اللي + +162 +00:11:24,430 --> 00:11:28,770 +يمكن نرتكبها إن نتعرف على ميكروبات مش in pure + +163 +00:11:28,770 --> 00:11:32,930 +culture، لازم تكون in pure culture، طيب عزلة + +164 +00:11:32,930 --> 00:11:36,750 +ميكروب؟بدأ أزرعه عشان بدأ أعمله sensitivity + +165 +00:11:36,750 --> 00:11:39,450 +testing، إيش يعني sensitivity testing؟ يعني أشوف + +166 +00:11:39,450 --> 00:11:43,450 +مدى حساسيته أو مقاومته لمين؟ للمضادات، في الحيوان، + +167 +00:11:43,450 --> 00:11:48,270 +برضه لازم نزرعه، harvesting cells، كلمة harvesting + +168 +00:11:48,270 --> 00:11:52,820 +يعني حصاد، الحصاد لمين بيكون؟ للمحصول، صح؟لما أنا + +169 +00:11:52,820 --> 00:11:56,000 +بدي خلايا، أنا قلت لك قبل شوية بدي أعمل vaccine صح؟ + +170 +00:11:56,000 --> 00:12:01,280 +أو بدي أعمل reagent باستخدام البكتيريا، فبدي خلايا + +171 +00:12:01,280 --> 00:12:05,300 +بكتيريا، فبزرعها عشان أكبرها وأنميها وأحصد + +172 +00:12:05,300 --> 00:12:11,920 +المحصول، الـharvesting itself، نيجي للوحة المهمة جدًا + +173 +00:12:11,920 --> 00:12:17,640 +جدا جدًا وهي طريقة تسمياتنا للبكتيريا، إيش المحاضرة + +174 +00:12:17,640 --> 00:12:22,050 +اللي كنتي؟ تسنيم، إيش؟ تسنيم الوحيدة ما كانت واحدة + +175 +00:12:22,050 --> 00:12:24,430 +اسمها في البلد .. في العالم كله تسنيم لوحدها؟ + +176 +00:12:24,430 --> 00:12:28,930 +ملايين؟ فيه؟ فيها نفس الاسم؟ لأ، لوحدها، لأ لوحدها + +177 +00:12:28,930 --> 00:12:34,030 +تسنيم لوحدها؟ لأ، طيب لو كان في عائلتك في كمان عمك + +178 +00:12:34,030 --> 00:12:38,330 +مسمى برضه تسنيم، تسنيم أنت بتختلف عنها صح ولا لأ؟ + +179 +00:12:38,330 --> 00:12:41,990 +فكيف نميزهم من بعض؟ هيك وهيك وهيك، خربطونهم في + +180 +00:12:41,990 --> 00:12:46,170 +عضو الاسم، اسمها واسم أبوها مظبوط، التالت والرابع + +181 +00:12:46,170 --> 00:12:52,130 +بعض الأحيان، ففي الميكروبات ولا تشبيه التصنيف، بدنا + +182 +00:12:52,130 --> 00:12:56,570 +نضمن إن أي فردين بيختلفوا عن بعض ينال اسمين + +183 +00:12:56,570 --> 00:12:59,550 +مختلفة، تفيد إن النظام اسمه binomial system + +184 +00:12:59,550 --> 00:13:04,590 +binomial system، نظام التسمية الثنائي اللي بيحتوي + +185 +00:13:04,590 --> 00:13:09,530 +على اسمين، نركز على اللوحة ونشوف فين دي، هنا حالة + +186 +00:13:09,530 --> 00:13:13,610 +اسمها الـgenus وحاجة اسمها Species، الـ + +187 +00:13:13,610 --> 00:13:17,290 +Staphylococcus هو الـgenus والـAureus هي الـ + +188 +00:13:17,290 --> 00:13:21,230 +Species، أعطيكم مثال، يعني في نفس السياق أنا بقول + +189 +00:13:21,230 --> 00:13:24,070 +Staphylococcus هو الـgenus هو اسم العائلة خلاص + +190 +00:13:24,070 --> 00:13:29,330 +يعني هو مش عائلة، لكن نفترض إنه عائلة، كتش فيه، والـ + +191 +00:13:29,330 --> 00:13:33,070 +Aureus هي الـSpecies هي البنت، البنت العائلة في + +192 +00:13:33,070 --> 00:13:35,590 +Staphylococcus epidermidis، Staphylococcus + +193 +00:13:35,590 --> 00:13:38,990 +saprophytic، Staphylococcus hemolyticus، مين اللي + +194 +00:13:38,990 --> 00:13:44,830 +اتغير؟ الأفراد الـspecies تمام، الـgenus هو هو، يبقى + +195 +00:13:44,830 --> 00:13:49,130 +لو نعرف الـgenus هو عبارة عن collection of species + +196 +00:13:49,130 --> 00:13:55,450 +مجموعة من إيه؟ من الـspecies المتشابهة، إشريشيا + +197 +00:13:55,450 --> 00:14:01,110 +كولاي، وين الـgenus؟ الإشريشيا، والكولاي الـspecies + +198 +00:14:01,110 --> 00:14:07,200 +الآن، سلمونيلا طايفي، Salmonella Genus والتيفي الـ + +199 +00:14:07,200 --> 00:14:10,660 +species طبعًا، الـSalmonella التيفي، تيفي موريوم، بارا + +200 +00:14:10,660 --> 00:14:15,340 +تيفي، انترادايدس، في مجموعة كبيرة من الأسماء تحت مين + +201 +00:14:15,340 --> 00:14:19,760 +تحت اللي هو الـSalmonella، اللي يهمني الآن أنا + +202 +00:14:19,760 --> 00:14:23,280 +إنكم عرفته إنه الاسم العلمي للبكتيريا مكون من + +203 +00:14:23,280 --> 00:14:28,220 +شقين، شق الأول Genus، الثاني space، الآن كيف كتبتهم + +204 +00:14:28,220 --> 00:14:34,000 +زمان، كان في خيارين يما تكتبهم، وأنا بعمل غلط هنا + +205 +00:14:38,240 --> 00:14:50,380 +بدنا نحاول نكتب بطريقة صحيحة، شايفين + +206 +00:14:50,380 --> 00:14:55,920 +إيش عمل تحتهم أنا؟ عمل تحتهم اللي هو كل كلمة منهم + +207 +00:14:55,920 --> 00:15:01,640 +خط، بسموها underline، وكلكم بتعرفوها في الـword مظبوط + +208 +00:15:01,640 --> 00:15:05,420 +أو في الـpowerpoint بنجيبها من المنطقة هذه، وفي + +209 +00:15:05,420 --> 00:15:09,200 +حاجة جانبها اسمها italic، صح اللي هي الخط المائل، + +210 +00:15:09,200 --> 00:15:13,900 +الاسم العلمي للـbacteria يبقى يكتب بطريقة اللي + +211 +00:15:13,900 --> 00:15:19,320 +مدانكم هذه، وهي طريقة قديمة، بطل .. بطل حد يستخدمها + +212 +00:15:19,320 --> 00:15:22,760 +الآن، الطريقة المستخدمة هي الطريقة اللي أمامكم هذه + +213 +00:15:22,760 --> 00:15:28,120 +حرف الأول capital، حرف الثاني أو الحرف الأول من + +214 +00:15:28,120 --> 00:15:33,540 +الكلمة الثانية small، والاثنين مكتوبين بخط إيش؟ + +215 +00:15:33,540 --> 00:15:37,420 +بينهم؟ + +216 +00:15:37,420 --> 00:15:40,620 +عاملين معالمين، إحنا اللي أنا أنا؟ علي تالي، إذا + +217 +00:15:40,620 --> 00:15:42,060 +تكتب بخط نائم + +218 +00:15:45,420 --> 00:15:48,780 +لو أنا رحت على أي بلد من بلدان العالم بيحكوا لغة + +219 +00:15:48,780 --> 00:15:52,260 +انجليزية، عربية، فرنسية، إيطالية، وقولت stuff you + +220 +00:15:52,260 --> 00:15:54,960 +look at is ours، هذا .. this is a universal name + +221 +00:15:54,960 --> 00:16:00,520 +اسم عالمي، وبنتكلم عن نفس الشخص، ما بنتكلمش عن أشخاص + +222 +00:16:00,520 --> 00:16:04,480 +مختلفين تمام؟ إذا لما بنقول binomial system هو + +223 +00:16:04,480 --> 00:16:10,460 +نظام تسمية، طبعًا هو نظام قديم واختُرع لتسمية + +224 +00:16:10,460 --> 00:16:14,220 +النباتات والحيوانات، مثلًا الفول اسمه Fischia Fava + +225 +00:16:14,930 --> 00:16:18,470 +الاليوم ستيفوم، والاليوم سيبا، الاليوم ستيفوم أعتقد + +226 +00:16:18,470 --> 00:16:22,610 +البصل، والاليوم سيبا .. ستيفوم اللي هو الثوم، موزا + +227 +00:16:22,610 --> 00:16:26,690 +سابنتوم اللي هو الموز، اعتسانونة اللي هو الجشطة هذه + +228 +00:16:26,690 --> 00:16:31,630 +أسماء برضه للنباتات، يعني فالبكتيريا نفس الشيء + +229 +00:16:31,630 --> 00:16:33,890 +Staphylococcus aureus, Escherichia coli, + +230 +00:16:34,170 --> 00:16:38,550 +Salmonella typhi، إذن هذا خدوا بالكم في رسائل + +231 +00:16:38,550 --> 00:16:41,310 +ماجيستير، مناقشات الطلبة، تعود ماجيستير في الميكروبيولوجي + +232 +00:16:41,310 --> 00:16:45,570 +أو أبحاث تخرج الـ .. الـ .. الـ .. بكريوز، لو وجد الاسم + +233 +00:16:45,570 --> 00:16:49,230 +اللي عليه مكتوب بطريقة خاطئة، بيسيبوا كل الموضوع و + +234 +00:16:49,230 --> 00:16:51,890 +يمسكوا فيها ليه كتبنا .. فبالتالي اللي يتعلم من + +235 +00:16:51,890 --> 00:16:55,230 +الآن وإحنا بنكتب يكتبه بطريقة الصحيحة، عارف الأول + +236 +00:16:55,230 --> 00:16:59,030 +capital من الـgenus والـ .. الـspecific وإن هو + +237 +00:16:59,030 --> 00:17:06,690 +اسمه الـ + +238 +00:17:06,690 --> 00:17:10,990 +general، الموضوع الأصعب والأعقد في هذه المحاضرة + +239 +00:17:10,990 --> 00:17:12,310 +اللي هو bacteria taxonomy أو bacteria + +240 +00:17:12,310 --> 00:17:16,850 +classification، هو كلهم تقريبًا بيعطوا نفس التصنيفات + +241 +00:17:16,850 --> 00:17:21,130 +وعودة إلى المكتبة، نذكركم بالمكتبة، المكتبات اللي + +242 +00:17:21,130 --> 00:17:26,790 +بتروح عليها تستعير الكتب، تم تصنيف الكتب إلى أظن + +243 +00:17:26,790 --> 00:17:30,710 +التصنيفين إنكم باللغة الإنجليزية وباللغة العربية + +244 +00:17:30,710 --> 00:17:34,270 +روحنا على اللغة الإنجليزية وجينا إن في تصنيفات + +245 +00:17:34,270 --> 00:17:40,510 +ثانية، في مراجع غير قابلة للاستعارة، ومراجع قابلة + +246 +00:17:40,510 --> 00:17:44,650 +للاستعارة، في عندنا تحت كل منهما في science + +247 +00:17:44,650 --> 00:17:47,970 +تصنيفات subject، في الـscience، في الـchemistry، في + +248 +00:17:47,970 --> 00:17:51,850 +الـphysics، في كده مظبوط، تصنيفات، إذا وردنا أكثر من + +249 +00:17:51,850 --> 00:17:56,170 +تصنيف أو أكثر من معيار يستخدم في التصنيف، فما هي + +250 +00:17:56,170 --> 00:18:00,130 +المعايير المستخدمة في التصنيف في علم الـ + +251 +00:18:00,130 --> 00:18:04,330 +microbiology واحد لـ Gramstein فاكرين كنا Gram + +252 +00:18:04,330 --> 00:18:06,610 +positive و Gram negative بعد كده كانت نقطة كتير + +253 +00:18:06,610 --> 00:18:11,470 +معنا الـ percentage of GC أعتقد كل كنت تعرفه لجوانين + +254 +00:18:11,470 --> 00:18:15,870 +سايتوسين والادينين ثايمين درجة القرابة للميكروبات + +255 +00:18:15,870 --> 00:18:21,430 +يمكن تحديدها من نسبة التشابه ما بين الـ GC content + +256 +00:18:21,430 --> 00:18:24,910 +خمسين في المية ستين في المية سبعين في المية الـ GC + +257 +00:18:24,910 --> 00:18:29,050 +content بيكون جراب لبعض الـ two organism تمام؟ إذا + +258 +00:18:29,050 --> 00:18:32,690 +كان واحد fifty percent الـ GC content وواحد twenty + +259 +00:18:32,690 --> 00:18:36,840 +percent دول المعاناة دمية متباعدين عن بعض growth + +260 +00:18:36,840 --> 00:18:41,580 +temperature شفنا بعض الميكروبات تنمو على low + +261 +00:18:41,580 --> 00:18:46,660 +-cyclophil و mesophil و ثرموخيه ability to form + +262 +00:18:46,660 --> 00:18:50,700 +heat stable، جونا spore، جونا spore former و non + +263 +00:18:50,700 --> 00:18:54,040 +-spore former زي البصيلة و الكريسترديوم، هدولة + +264 +00:18:54,040 --> 00:18:57,120 +اندو-spore former وباجين non-indu-spore former + +265 +00:18:58,050 --> 00:19:02,530 +الإلكترون acceptors for respiration، شوفنا + +266 +00:19:02,530 --> 00:19:04,890 +الأكسجين في الـ aerobic respiration، شوفنا الـ + +267 +00:19:04,890 --> 00:19:08,410 +inorganic compounds في الـ anaerobic respiration، + +268 +00:19:08,410 --> 00:19:12,030 +شوفنا الـ fermentation فيها، organic compound كفانة + +269 +00:19:12,030 --> 00:19:15,590 +إلكترون acceptphotosynthetic ability + +270 +00:19:15,590 --> 00:19:17,770 +photosynthetic bacteria و non-photosynthetic + +271 +00:19:17,770 --> 00:19:21,210 +bacteria motility motile من الموتالي سا قبل لحظة + +272 +00:19:21,210 --> 00:19:23,770 +شفنا الـ motile cell shape بسيلاي و لا كوكساي و لا + +273 +00:19:23,770 --> 00:19:27,050 +spiral و لا curved بطبعا cell shape تشمل معاها + +274 +00:19:27,050 --> 00:19:30,370 +كمان الـ arrangement ببعض الخلاقات ability to use + +275 +00:19:30,370 --> 00:19:32,970 +various carbon and nitrogen sources أعتقد أنه ممكن + +276 +00:19:32,970 --> 00:19:37,070 +كارثة المحاضرة كلها أصلا مصابة على أنه في اختلافات + +277 +00:19:37,070 --> 00:19:40,010 +في البكتيريا من قدرتها على استخدام المراكبات + +278 +00:19:40,010 --> 00:19:43,660 +المختلفة بالضبط زي المكتبة دخلنا نجينا مجموعة + +279 +00:19:43,660 --> 00:19:48,640 +تصنيفات، يعني الكتاب لا يخضع لمعيار واحد، أنه + +280 +00:19:48,640 --> 00:19:51,940 +انجليزي او عربي، لأ خضع لانجليزي او عربي و أنه + +281 +00:19:51,940 --> 00:19:54,700 +science ولا health و جوا الـ health او الـ science + +282 +00:19:54,700 --> 00:19:59,360 +خضع لانه chemistry ولا physics ولا .. ولا biology، + +283 +00:19:59,360 --> 00:20:02,280 +مظبوط؟ ففي مجموعة كبيرة من المعيار المستخدمة في + +284 +00:20:02,280 --> 00:20:06,350 +التصنيف ability to use various carbon monoxide + +285 +00:20:06,350 --> 00:20:09,430 +sources, special nutritional requirement شوفنا + +286 +00:20:09,430 --> 00:20:12,830 +بكتيريا تحتاج إلى x and d factors سميناها fast and + +287 +00:20:12,830 --> 00:20:18,010 +fast وبكتيريا الـ minimum requirement بتجديها + +288 +00:20:18,010 --> 00:20:25,850 +سميناها non-fast and fast نجي الآن لمجلد أو مؤلف + +289 +00:20:25,850 --> 00:20:27,890 +مشهور جدا في علم الـ microbiology أو في علم + +290 +00:20:27,890 --> 00:20:31,710 +البكتيروري بالذات اسمه bergesmanuel of systemic + +291 +00:20:31,710 --> 00:20:35,230 +bacteriology bergesmanuel of systemic bacteriology + +292 +00:20:35,230 --> 00:20:41,430 +كلمة systemic لها علاقة بالتصنيف خدوا بالكم لأن + +293 +00:20:41,430 --> 00:20:45,430 +التصنيف عملية نظامية أساسا عملية نظامية معتمدة على + +294 +00:20:45,430 --> 00:20:47,390 +فبعض الحياة بيسموه systemic + +295 +00:20:50,760 --> 00:20:55,220 +اللوحات اللي امامنا هدول هنحفظهم؟ لأ مش هنحفظهم طب + +296 +00:20:55,220 --> 00:20:58,460 +و ليش بندرسهم؟ مدام انتوا عارفين انتوا يعني مولودة + +297 +00:20:58,460 --> 00:21:02,500 +يعني بالتأكيد قالية مبرمجة في أدمغاتكم انه مدام مش + +298 +00:21:02,500 --> 00:21:06,300 +في الامتحان بيجيباش للدراسة لأول الدراسة و لكليس + +299 +00:21:06,300 --> 00:21:11,420 +الامتحان لأنه تلقائي و احنا بيدرس فيهم هنستفيد + +300 +00:21:11,420 --> 00:21:15,760 +للمستقبل فهنلاجح حالنا هنحفظهم لامتحان بس مش + +301 +00:21:15,760 --> 00:21:20,330 +امتحان المنتقل طيب المجموعين احنا في الـbergers + +302 +00:21:20,330 --> 00:21:24,710 +manual المجموعات الى تلات تلاتين section تلات + +303 +00:21:24,710 --> 00:21:29,590 +تلاتين section همور عليهم مرور نتعرف عليهم تعرف + +304 +00:21:29,590 --> 00:21:35,230 +سريع و بسيط منهم قد نجف عليه لحظات و البعض الاخر + +305 +00:21:35,230 --> 00:21:40,860 +مش هنجف عليه لإن اسمه بيكون وصف لحاله هده + +306 +00:21:40,860 --> 00:21:45,660 +Spirochetes Spirochetes وبين جثين هي الـ gram + +307 +00:21:45,660 --> 00:21:50,680 +negative spiral shaped cells خلايا ده شكل لولة + +308 +00:21:50,680 --> 00:21:55,080 +with endoflagella أول مرة أتكلم على endoflagella + +309 +00:21:55,080 --> 00:22:01,000 +الـ endoflagella فاكرين ان كانت الـ endoflagella على + +310 +00:22:01,000 --> 00:22:08,040 +الـ bacteria ظاهرة اما على طرف او طرفين او على + +311 +00:22:08,040 --> 00:22:12,620 +الألج في الأخر كانتهي عبارة عن زوائد البكتيرى اللى + +312 +00:22:12,620 --> 00:22:15,620 +بتحدث عنها شوية بتختلف احنا قولنا هنا قبل شوية + +313 +00:22:15,620 --> 00:22:22,200 +spiral بدنا نضخمها شوية اعتبروها + +314 +00:22:22,200 --> 00:22:28,260 +بدون cell wall بدون cell wall هان طالع منها فلاجلة + +315 +00:22:28,260 --> 00:22:35,780 +بس بدل ما يطلع برا وبعدين + +316 +00:22:35,780 --> 00:22:36,180 +جميل + +317 +00:22:43,490 --> 00:22:48,610 +الان صار عندى مباني الـ cell membrane و الـ cell + +318 +00:22:48,610 --> 00:22:55,690 +wall فى عندى خيط marine marine زى الـ rubber تمام + +319 +00:22:55,690 --> 00:23:00,410 +البكتيريا شكلها لهو اللى بيه لو انا مسكت طرفها + +320 +00:23:00,410 --> 00:23:05,630 +منها لهو او هى اخدت قرار اخدت قرار انه بيتحرك تمال + +321 +00:23:05,630 --> 00:23:09,970 +twitching twitching يعني بتلف لو تركت المنطقة اللى + +322 +00:23:09,970 --> 00:23:14,720 +حاسس فيها هتروح معاها لإن هو Marina فلما يتحرك حول + +323 +00:23:14,720 --> 00:23:18,660 +المحور تبعها، إيش بيصير فيها؟ تتحرك إلى الأمام أو + +324 +00:23:18,660 --> 00:23:23,720 +إلى الخلف وبالتالي it moves by rotating itself + +325 +00:23:23,720 --> 00:23:28,600 +around its axis نفس المحور تبعها، تتحرك وبتحرك من + +326 +00:23:28,600 --> 00:23:32,200 +خلاله، إن هال اللي عنده فلجلة ولا اسم تاني اسمها + +327 +00:23:32,200 --> 00:23:35,400 +axial filament + +328 +00:23:43,030 --> 00:23:48,010 +إذن الآن بنقدر نقول إنه صار فينا معارفة بمجموعة + +329 +00:23:48,010 --> 00:23:50,530 +اسمها Spirocates والـ Spirocates كنا نقولها بقى + +330 +00:23:50,530 --> 00:23:53,610 +بيبقى Ground Negative Spiral Organism وقالها Endo + +331 +00:23:53,610 --> 00:23:57,310 +Flagellate بتاخدوا مثال عليها؟ خدوا ليكم مثال + +332 +00:23:57,310 --> 00:24:04,410 +تريبونيما باليدوم تريبونيما حتمر معانا كمان شوية + +333 +00:24:04,410 --> 00:24:08,170 +تريبونيما وبتسبب مرض اسمه مرض الـ syphilis مرض الـ + +334 +00:24:08,170 --> 00:24:11,190 +syphilis طيب + +335 +00:24:12,380 --> 00:24:18,340 +مرض الـ syphilis المجموعة + +336 +00:24:18,340 --> 00:24:24,840 +الرقم اتنين هي عبارة عن aerobic او ميكرو ايروفيليك + +337 +00:24:24,840 --> 00:24:30,440 +مقطع + +338 +00:24:30,440 --> 00:24:35,140 +الهيليكل فيبريويد فيبريويد يعني شكلها زي الفيبريو + +339 +00:24:35,140 --> 00:24:39,000 +أو هيليكل يعني في واحدة من اتنتين يا اما هيليكل يا + +340 +00:24:39,000 --> 00:24:44,480 +اما فيبريويد ground negative bacteria رقم تلاتة non + +341 +00:24:44,480 --> 00:24:49,100 +motile or rarely motile تشوفوا بعض الأحيان التصنيف + +342 +00:24:49,740 --> 00:24:54,060 +يعني في الكتب هذا Biology بيولوجي طب لو أتينا واحد + +343 +00:24:54,060 --> 00:24:57,440 +في الـ physiology هو ممكن يكون health وممكن يكون + +344 +00:24:57,440 --> 00:25:00,420 +biology فممكن أحط نسخة في الـ biology و أحط نسخة + +345 +00:25:00,420 --> 00:25:08,700 +هنا في الـ physiology فممكن أحط + +346 +00:25:08,700 --> 00:25:09,300 +فممكن أحط نسخة في الـ biology و أحط نسخة هنا في الـ + +347 +00:25:09,300 --> 00:25:09,700 +physiology فممكن أحط نسخة في الـ biology و أحط نسخة + +348 +00:25:09,700 --> 00:25:10,060 +هنا في الـ physiology فممكن أحط نسخة في الـ biology + +349 +00:25:10,060 --> 00:25:11,740 +و أحط نسخة هنا في الـ physiology فممكن أحط نسخة في + +350 +00:25:11,740 --> 00:25:14,600 +الـ biology و أحط نسخة هنا في الـ physiology فممكن + +351 +00:25:14,600 --> 00:25:17,560 +أحط نسخة في الـ biology و أحط نسخة هنا في الـ + +352 +00:25:17,560 --> 00:25:20,340 +physiology فممكن أحط نسخة في الـ biology تصنيف الكتب + +353 +00:25:20,340 --> 00:25:23,380 +قمر سهل، يعني حدث كبير أو معروف أو واضح بالنسبة + +354 +00:25:23,380 --> 00:25:26,340 +لنا، بس بالنسبة للبكتيريا لإن معلوماتنا بتزيد و + +355 +00:25:26,340 --> 00:25:31,880 +بتتغير بستمرة فحتلاجوا تغيير في التصنيفات بستمرة، + +356 +00:25:31,880 --> 00:25:37,600 +أعطيكم مثل هذافيه كانت بكتيريا اسمها Enterobacter + +357 +00:25:37,600 --> 00:25:42,960 +Sakazaki وبكتيريا بانتباهها جديدة يعني عملنا عليها + +358 +00:25:42,960 --> 00:25:46,380 +دراسة في الجامعة هنا واحنا مكتب في الـ paper تبعت + +359 +00:25:46,380 --> 00:25:49,780 +الموضوع لجينا انه صار فيه تصنيفات اتغير الاسم صار + +360 +00:25:49,780 --> 00:25:53,540 +اسمها Chronobacter Sakazaki مين اللي اتغير؟ + +361 +00:25:57,850 --> 00:26:02,450 +اليرسينيا بيستس كان اسمها pasturella biestes + +362 +00:26:02,450 --> 00:26:09,490 +فغالبا ممكن تكون التغيير فقط نقل البكتيريا من + +363 +00:26:09,490 --> 00:26:13,950 +genus إلى genus طب و احنا ايش كنا؟ كنا مش عارفين؟ + +364 +00:26:13,950 --> 00:26:16,430 +لما حطينا البكتيريا ده في genus معين كنا مش + +365 +00:26:16,430 --> 00:26:21,840 +عارفين؟ نعم كنا مش عارفين معلوماتنا كانت قليلة لما + +366 +00:26:21,840 --> 00:26:27,040 +زادت معلوماتنا اثبتت ان هذا الـ microbe لا يستحق ان + +367 +00:26:27,040 --> 00:26:31,040 +يكون في هذا التصنيف وجب ان ينقل اما اسم جديد او ان + +368 +00:26:31,040 --> 00:26:35,680 +ينقل حتى لـ genus اخر فعشان اقولكم في تكسونومي في + +369 +00:26:35,680 --> 00:26:40,720 +نوع من الصعوبة وفي نفس الوجد في انا تغيرات مستمرة + +370 +00:26:40,720 --> 00:26:43,520 +في هذا المشكلة + +371 +00:26:46,620 --> 00:26:51,560 +Aerobic rods and cocci, facultatively anaerobic + +372 +00:26:51,560 --> 00:26:57,480 +gram negative rods, anaerobic + +373 +00:26:57,480 --> 00:27:01,320 +gram negative straight curved and helical rods + +374 +00:27:06,470 --> 00:27:10,190 +dissimilatory sulfate أو اسم الأشهر لها sulfur + +375 +00:27:10,190 --> 00:27:15,030 +-reducing بكتيريا sulfur-reducing واضح من اسمها إن + +376 +00:27:15,030 --> 00:27:19,510 +هذه البكتيريا تتغذى على المركبات الكبريتية أنا + +377 +00:27:19,510 --> 00:27:23,150 +aerobic gram negative cocci دا ريكتسياس و + +378 +00:27:23,150 --> 00:27:26,070 +كلاميدياس هذا أول مرة تسمعوا فيهم شيلوا الـ S من + +379 +00:27:26,070 --> 00:27:30,590 +كل واحدة منهم و هتعطيهم خط كلاميديا و ريكتيسيا + +380 +00:27:30,590 --> 00:27:37,540 +كلاميديا و ريكتيسيا إيش منام دولة؟ هدول the only + +381 +00:27:37,540 --> 00:27:41,640 +بالنسبة لنا المعروفين the only obligate + +382 +00:27:41,640 --> 00:27:46,760 +intracellular bacteria، إيش يعني؟ هي البكتيريا + +383 +00:27:46,760 --> 00:27:51,640 +الوحيدة المُطفلة طفيلية من البكتيريا، بتذكرنا + +384 +00:27:51,640 --> 00:27:55,440 +من مين المصطلح؟ من الـ viruses، الـ viruses هم + +385 +00:27:55,440 --> 00:27:59,600 +obligate intracellular viruses وبالتالي هدول + +386 +00:27:59,600 --> 00:28:04,200 +الميكروبات أقرب ما يكون في العلاقة ما بينهم بين + +387 +00:28:04,200 --> 00:28:06,900 +الخلايا إلى الفيروسات لأنهم obligate intracellular + +388 +00:28:06,900 --> 00:28:10,840 +برزات أنا للأسف مش مكتوب كلمة obligate هي الأصل + +389 +00:28:10,840 --> 00:28:18,020 +أنه إن حطينا obligate intracellular برزات لش + +390 +00:28:18,020 --> 00:28:24,720 +أنا مصرح أحط obligate لإن هناك facultative نعم مثلا + +391 +00:28:24,720 --> 00:28:28,040 +زي السالمونيلا ممكن تعيش معايشة حرة و ممكن تعمل + +392 +00:28:28,040 --> 00:28:33,270 +intra ... بصرا .. intracellular برضه طبعا ال + +393 +00:28:33,270 --> 00:28:37,230 +rickettsia و الـ chlamydia ميكروبات مهمة بتسبب + +394 +00:28:37,230 --> 00:28:41,210 +أمراض، قلنا في منها الـ rickettsia بتسبب أمراض في + +395 +00:28:41,210 --> 00:28:45,310 +.. في فلسطين اللي هو murine typhus مش الـ typhoid، + +396 +00:28:45,310 --> 00:28:50,910 +murine typhus و بينتقل عن طريق الحشرات و موجود أنه + +397 +00:28:50,910 --> 00:28:54,550 +في .. في .. في غزة بيتشر في شهر سبعة إلى شهر تسعة + +398 +00:28:54,550 --> 00:28:57,010 +تقريبا أو شهر عشر يعني .. يعني الفترة الزمنية اللي + +399 +00:28:57,010 --> 00:29:02,770 +احنا موجودين فيها الآن، الكلاميديا معروف عنها فاكرين + +400 +00:29:02,770 --> 00:29:07,440 +السيلان المزمن؟ السيلان المزمن إيش بيقول عنها؟ لأ + +401 +00:29:07,440 --> 00:29:10,440 +نفسنا كنا نرى عنها كنا من أشهر .. من أشهر + +402 +00:29:10,440 --> 00:29:12,700 +الميكروبات المسببة للـ STDs اللي هي sexual + +403 +00:29:12,700 --> 00:29:16,220 +transmitted diseases تنتقل عن طريق الجنس كنا هي .. + +404 +00:29:16,220 --> 00:29:19,960 +هي رقم اثنين مين رقم واحد؟ رقم واحد الكلاميديا رقم + +405 +00:29:19,960 --> 00:29:24,860 +واحد الكلاميديا طبعا تبتدأ الأمراض الأخرى غير الـ .. ال + +406 +00:29:24,860 --> 00:29:27,200 +.. الـ .. الأمراض الجنسية ممكن تاما الحادثة اسمها + +407 +00:29:27,200 --> 00:29:31,500 +Trachoma Trachoma بالعربي تراخوما و Trachoma هو + +408 +00:29:31,500 --> 00:29:36,840 +ما بيصيب العين و قد يؤدي إلى الـ blindness في قرى في + +409 +00:29:36,840 --> 00:29:41,880 +أفريقيا يمكن أن يكون نصف السكان فقدوا البصر لأن + +410 +00:29:41,880 --> 00:29:47,100 +عندهم الكلاميديا متشرقة بشكل كبير، يدعى ريكتيسيا + +411 +00:29:47,100 --> 00:29:53,500 +و الكلاميديا كاسمها مهمين جدا في عالم الطب، المايكو + +412 +00:29:53,500 --> 00:29:56,880 +بلازما ما تقولوليش ما سمعتش فيها هذه، ما سمعت فيها + +413 +00:29:56,880 --> 00:30:02,860 +هي الأصغر، دي هي الأصغر بكثير وهي wall-less أو + +414 +00:30:02,860 --> 00:30:08,720 +lacks a defined cell wall، فيها إنها حاسمة + +415 +00:30:08,720 --> 00:30:16,390 +في الـ endosymbiosis و Endosymbionts يعني بكتيريا تعيش + +416 +00:30:16,390 --> 00:30:20,990 +في خلايا كائنات أخرى زي مثلا البكتيريا العقدية + +417 +00:30:20,990 --> 00:30:25,470 +الجدرية اللي بنشوفها في نباتات زي الفول، الرقم + +418 +00:30:25,470 --> 00:30:28,030 +اثنا عشر اللي هم الـ gram-positive cocci مشهورة هذه زي + +419 +00:30:28,030 --> 00:30:31,350 +الستافيلوكوكاس و الستربتوكوكاس، الـ endospore-forming + +420 +00:30:31,350 --> 00:30:35,170 +بكتيريا تبعت الـ gram-positive bacilli زي الباسيلس و + +421 +00:30:35,170 --> 00:30:39,940 +الكلستريديوم و طبعا مجموعتين مهمين جدا regular non + +422 +00:30:39,940 --> 00:30:44,860 +-sporing gram-positive bacilli كلمة regular يعني + +423 +00:30:44,860 --> 00:30:49,200 +شكلها منتظم، في irregular شكلها غير منتظم + +424 +00:30:49,200 --> 00:30:55,940 +الميكوبكتيريا، الميكوبكتيريا إلها اسمين من أشهر + +425 +00:30:55,940 --> 00:30:59,160 +الميكروبات على كوكب الأرض Mycobacterium + +426 +00:30:59,160 --> 00:31:03,060 +tuberculosis و بتسبب مرض السل الرئوي أو الدرن + +427 +00:31:03,060 --> 00:31:07,900 +Mycobacterium tuberculosis فلسطين أو غزة بالذات + +428 +00:31:07,900 --> 00:31:13,900 +بتعاني سنويا من خمس عشرة تقريبا خمس عشرة حالة سنويا + +429 +00:31:13,900 --> 00:31:19,560 +فقط يعني العدد منخفض لأن في فلسطين العدد منخفض لأن + +430 +00:31:19,560 --> 00:31:25,040 +أن بتعرفوا اللي يسموها اللقاح التخريصة اللي في + +431 +00:31:25,040 --> 00:31:33,520 +الكتف اسم اللقاح BCG احنا الآن في قطاع غزة الأرقام + +432 +00:31:33,520 --> 00:31:38,400 +تبعنا تضاهي أو أفضل من بعض الدول الأوروبية بسبب + +433 +00:31:38,400 --> 00:31:41,380 +أن احنا لسه ما زلنا بناخد اللقاح هم بطلوا ياخدوا + +434 +00:31:41,380 --> 00:31:45,720 +اللقاح ليه بطلوا ياخدوا اللقاح واحنا ناخدوا اللقاح؟ + +435 +00:31:45,720 --> 00:31:50,900 +هم بطلوا لأنه لقوا نسبة الإصابة من التطعيم نفسه + +436 +00:31:50,900 --> 00:31:53,480 +يعني الناس اللي بتنصاب بعد ما بتطعمه يعني من + +437 +00:31:53,480 --> 00:31:58,140 +التطعيم السابق للتطعيم أعلى من اللي بيكسبوا الإصابة من + +438 +00:31:58,140 --> 00:32:03,240 +natural sources فجاءه بنواجه طبعا أنه صار تحسن في + +439 +00:32:03,240 --> 00:32:07,120 +الصحة العامة و مستوى التغذية في .. في .. في + +440 +00:32:07,120 --> 00:32:12,280 +البلدان الأوروبية فهذا الأمر تقريبا شبه انعدم احنا + +441 +00:32:12,280 --> 00:32:18,540 +لسه فيه مشاكل تغذية و بالتالي WHO قالت خلّي التطعيم + +442 +00:32:18,540 --> 00:32:22,720 +جاريا و بالتالي هو جوفرنا حماية و نسبة من الخمس عشر حال + +443 +00:32:22,720 --> 00:32:24,980 +اللي بحكيلكوا عنها سنويا نسبة بتكون ناجمة عن + +444 +00:32:24,980 --> 00:32:30,800 +الفعالية عن اللقاح بتصيب الأطفال اللي بتطعمه + +445 +00:32:33,500 --> 00:32:37,860 +البكتيريا اللي اعتقدنا أنه في أوروبا و أمريكا قضية + +446 +00:32:37,860 --> 00:32:43,400 +عليها ظهرت مرة أخرى و ظهرت بشكل خطير جدا و هو شكل + +447 +00:32:43,400 --> 00:32:47,760 +بنسميه احنا multi-drug resistant يعني سلالات + +448 +00:32:47,760 --> 00:32:54,160 +مقاومة للعديد من المضادات الحيوية فعادت عودة يعني + +449 +00:32:54,160 --> 00:32:59,140 +أشرس من ذي قبل فهو بالتالي الآن في جهود كبيرة لحل + +450 +00:32:59,140 --> 00:33:03,370 +الإشكالات المتعلقة بهذا الـ microbe طيب الـ microbe + +451 +00:33:03,370 --> 00:33:06,670 +الثاني الاسم الثاني و أنه Mycobacterium + +452 +00:33:06,670 --> 00:33:09,630 +leprae اللي هي اللي هنتحدث عنها الثاني + +453 +00:33:09,630 --> 00:33:13,510 +Mycobacterium leprae و leprae اللي هو من جامع كلمة + +454 +00:33:13,510 --> 00:33:19,470 +leprosy، leprosy مار معاكم جودام؟ جودام الرسول + +455 +00:33:19,470 --> 00:33:23,390 +صلى الله عليه و سلم بيقول في حادثة الشريف لا عدوى + +456 +00:33:23,390 --> 00:33:29,250 +ولا طيرة ولا همه ولا صفر وفرة من المجذوم فرّه كمن + +457 +00:33:29,250 --> 00:33:35,050 +الأسد المجذوم هو الإنسان المصاب بالجذام و الجذام + +458 +00:33:35,050 --> 00:33:39,090 +هو اللي بتسببه البكتيريا اللي اسمها Mycobacterium + +459 +00:33:39,090 --> 00:33:47,890 +leprae الجذام مشهور أكثر وين؟ في الهند في باكستان في + +460 +00:33:47,890 --> 00:33:53,110 +بنغلاديش في الدول الآسيوية جنوب شرق آسيا هذه + +461 +00:33:53,110 --> 00:33:56,410 +بلد بلدها مشهورة بالجذام الجزيرة العربية كان فيها + +462 +00:33:56,410 --> 00:33:59,870 +جذام فلسطين أنا شوفت حالة واحدة فقط فيها جذام + +463 +00:33:59,870 --> 00:34:02,390 +مهاجرة من برا يعني مش من هنا أساسا + +464 +00:34:07,700 --> 00:34:10,720 +لحد الآن بكتيريا الجذام لم نستطع تنميتها، لم + +465 +00:34:10,720 --> 00:34:15,100 +نستطع تنميتها، وتوصف الـ Mycobacteria الصينفين + +466 +00:34:15,100 --> 00:34:19,810 +اللي أجابتهم أنا بـ slow growing bacteria فاكرين الـ + +467 +00:34:19,810 --> 00:34:23,250 +equalize؟ فاكرين الـ equalize؟ فاكرين الـ equalize؟ + +468 +00:34:23,250 --> 00:34:23,750 +فاكرين الـ equalize؟ فاكرين الـ equalize؟ فاكرين الـ + +469 +00:34:23,750 --> 00:34:24,050 +equalize؟ فاكرين الـ equalize؟ فاكرين الـ equalize؟ + +470 +00:34:24,050 --> 00:34:26,110 +فاكرين الـ equalize؟ فاكرين الـ equalize؟ فاكرين الـ + +471 +00:34:26,110 --> 00:34:28,530 +equalize؟ فاكرين الـ equalize؟ فاكرين الـ equalize؟ + +472 +00:34:28,530 --> 00:34:28,950 +فاكرين الـ equalize؟ فاكرين الـ equalize؟ فاكرين الـ + +473 +00:34:28,950 --> 00:34:29,030 +equalize؟ فاكرين الـ equalize؟ فاكرين الـ + +474 +00:34:29,030 --> 00:34:31,350 +equalize؟ فاكرين الـ equalize؟ فاكرين الـ + +475 +00:34:31,350 --> 00:34:37,950 +equalize؟ فاكرين الـ equalize؟ + +476 +00:34:37,950 --> 00:34:40,390 +فاكرين الـ equalize؟ + +477 +00:34:41,900 --> 00:34:45,900 +Cardioform وهي acid-fast بكتيريا، إيش يعني acid + +478 +00:34:45,900 --> 00:34:49,060 +-fast بكتيريا؟ إذا فاكرين لما تحدثت عن الـ gram + +479 +00:34:49,060 --> 00:34:52,760 +-stain بالضبط، لما تحدثنا عن الـ gram-stain، كنا + +480 +00:34:52,760 --> 00:34:55,960 +بيقسم البكتيريا لـ gram بُسط و gram نيجاتيف، الآن + +481 +00:34:55,960 --> 00:35:00,680 +أكبرتو شوية أنتو، قوللكوا أنه لا يوجد أو أو + +482 +00:35:00,680 --> 00:35:03,380 +بالأحرى الـ gram-stain لا يستطيع سببها تكون + +483 +00:35:03,380 --> 00:35:07,520 +البكتيريا، ليش؟ في نوع من البكتيريا عنده طبقة من الـ + +484 +00:35:07,520 --> 00:35:10,860 +wax celibate يصعب صباغة البكتيريا عشان نصبغها + +485 +00:35:10,860 --> 00:35:15,320 +بنسخن البكتيريا بنحط الصبغة و بنسخن البكتيريا تدخل + +486 +00:35:15,320 --> 00:35:18,580 +الصبغة لما نشيل الصبغة نعمل الـ decolourization + +487 +00:35:18,580 --> 00:35:24,960 +بيكون في صعوبة خالص حتى لو ضفنا مزيد للون قوي + +488 +00:35:24,960 --> 00:35:27,860 +فاكرين احنا نشيل الصبغة داخل الجراز الثاني إيثانول + +489 +00:35:27,860 --> 00:35:36,400 +هنت بستخدم خلطة من الإيثانول و الـ sulfuric acid و + +490 +00:35:36,400 --> 00:35:40,740 +الحمض و الحمض المعروف عنه أنه بيزيل اللون أكثر من + +491 +00:35:40,740 --> 00:35:45,000 +الكحول فاسمع الجملة دي، اسمع الجملة و بعدين نفسرها + +492 +00:35:45,000 --> 00:35:48,400 +قطع قطع عشان نقولكوا إيش معناه acid fast bacteria + +493 +00:35:56,180 --> 00:36:00,360 +but once stained they are difficult to decolorize + +494 +00:36:00,360 --> 00:36:04,400 +even in the presence of strong acid، القطعة الأولى + +495 +00:36:04,400 --> 00:36:07,800 +بتقول صعبة الصباغة لكن ماكنها مستحيلة صباغتها + +496 +00:36:07,800 --> 00:36:12,350 +بالتسخين But once stained, it is difficult to + +497 +00:36:12,350 --> 00:36:19,030 +decolorize، حتى في وجود حامض قوي، اسمها الجملة + +498 +00:36:19,030 --> 00:36:24,190 +اللي بتقول acid fast bacteria، بكتيريا مقاومة + +499 +00:36:24,190 --> 00:36:31,810 +للحمض، fast معناها صائم، fasting معناها صيام، + +500 +00:36:36,790 --> 00:36:39,790 +فبالتالي بنقول اللي هو الوصف الدقيق اللي باللغة + +501 +00:36:39,790 --> 00:36:44,510 +العربية الصلبة المقاومة للحمض بمعنى المقاوم للإزالة + +502 +00:36:44,510 --> 00:36:49,790 +بالحمض طبعا؟ فهناك أنواع من البكتيريا إذا صورناها + +503 +00:36:49,790 --> 00:36:53,090 +بـ acid-fast-stain هي الصواب، إزالة فنسميها acid + +504 +00:36:53,090 --> 00:36:56,530 +-fast-bacteria، acid-fast-bacteria بينما اللي بيزال + +505 +00:36:56,530 --> 00:36:59,970 +منها اللون العادي يعني نسميها non acid-fast + +506 +00:36:59,970 --> 00:37:02,850 +-bacteria، بينما لو كنا نقول gram positive و gram + +507 +00:37:02,850 --> 00:37:05,230 +negative بنقول acid-fast و non acid-fast + +508 +00:37:09,000 --> 00:37:13,020 +anoxygenic phototrophic bacteria، anoxygenic يعني في + +509 +00:37:13,020 --> 00:37:18,560 +غياب الأكسجين، و بتعمل photosynthesis، oxygenic photo + +510 +00:37:18,560 --> 00:37:23,580 +synthetic bacteria، زي الـ cyanobacteria، لو حطينا ميه + +511 +00:37:23,580 --> 00:37:28,440 +في مرتبان، plastic + +512 +00:37:36,290 --> 00:37:40,850 +اللون الأخضر هذا عبارة عن cyanobacteria، cyanobacteria + +513 +00:37:40,850 --> 00:37:46,170 +bacteria هو بعض الطحالب، عشان هي التنكات الميه اللي + +514 +00:37:46,170 --> 00:37:49,690 +فوق اللي بتكون في الإضاءة، إيش بيكون اللون هذا؟ + +515 +00:37:54,010 --> 00:37:58,530 +لكن غير منفذة للضوء، غير منفذة يعني بتكون معتمة، + +516 +00:37:58,530 --> 00:38:01,970 +بتكون معتمة، مش شفافة، ما في لون أبيض اللي بيكون + +517 +00:38:01,970 --> 00:38:06,710 +شفافة، بيبقى لون أبيض معتم، أسود معتم، أخضر معتم، + +518 +00:38:06,710 --> 00:38:11,530 +أزرق معتم، المهم إنه ما ينفذش من الضوء، Aerobic + +519 +00:38:11,530 --> 00:38:14,430 +chemolithotrophic bacteria and associated organisms + +520 +00:38:14,430 --> 00:38:17,130 +كلمة associated organisms معناها بحكي إنه هذا + +521 +00:38:17,130 --> 00:38:23,750 +عبارة عن مجموعة، و الحقنا بيها مجموعة أخرى، رقم 21 + +522 +00:38:23,750 --> 00:38:28,670 +غريبة، budding and/or appendaged bacteria، يعني + +523 +00:38:28,670 --> 00:38:34,670 +بكتيريا بيكون لها براعم أو لها زوائد، لها كلمة + +524 +00:38:34,670 --> 00:38:40,330 +appendaged معناها زوائد، sheathed bacteria، بكتيريا + +525 +00:38:40,330 --> 00:38:49,900 +لها أغلفة أو لها طبقات، رقم 23 فيها حاجة حلوة، non + +526 +00:38:49,900 --> 00:38:54,040 +-photosynthetic، non-fruiting، + +527 +00:38:54,040 --> 00:38:59,620 +الكلمة fruiting body مصطلح صحيح، بنجيبه واحنا من + +528 +00:38:59,620 --> 00:39:04,180 +وين من الفطريات، أنتو بتعرفوا الفطر المشروم، + +529 +00:39:04,180 --> 00:39:06,920 +المشروم اللي احنا بنشوفه هذا الـ fruiting body، هذا + +530 +00:39:06,920 --> 00:39:10,260 +الـ fruiting body، لكن على فكرة لو أنتو شفتوا + +531 +00:39:10,260 --> 00:39:15,940 +المشروم هو طالع في الأرض في فصل الشتاء، لما ت...ت + +532 +00:39:15,940 --> 00:39:19,400 +...تخلعوا المشروم هذا، ابقوا عصاية صغيرة أو عود + +533 +00:39:19,400 --> 00:39:22,300 +صغيرة، حاولوا تحركوا التربة اللي حواليها، هتلاقوا + +534 +00:39:22,300 --> 00:39:25,860 +آلاف، ملايين من خيوط بيضاء في المنطقة، هذا هو + +535 +00:39:25,860 --> 00:39:29,480 +الفطر، الجزء اللي طويل هذا اسمه الـ fruiting body، + +536 +00:39:29,480 --> 00:39:32,260 +في بعض أنواع من البكتيريا عاملها fruiting body و + +537 +00:39:32,260 --> 00:39:35,520 +من بعض الآخر اللي مش هو المهم، أنا عندي كلمة + +538 +00:39:35,520 --> 00:39:38,870 +gliding هي المهمة، البكتيريا احنا قلناها قبل شوية + +539 +00:39:38,870 --> 00:39:43,530 +إيه ما متحركة، إيه ما غير متحركة، واتكلمنا أول محاضرات + +540 +00:39:43,530 --> 00:39:47,870 +إنه متحركة بـ flagella، واليوم يعني اه قلنا لكم إنه + +541 +00:39:47,870 --> 00:39:51,390 +لا يا بنات في بكتيريا متحركة بس بـ endoflagella + +542 +00:39:51,390 --> 00:39:54,550 +طبعا يعني كل مرة كنا بنكبر شوية و بنكتشف إن + +543 +00:39:54,550 --> 00:40:00,270 +الحقيقة مش مطلقة، إن المعلومات تبعتنا مش مطلقة، يعني + +544 +00:40:00,270 --> 00:40:02,250 +إما flagella يبقى بدون flagella، لأ، لجينا + +545 +00:40:02,250 --> 00:40:05,430 +endoflagella، بالمرحلة لجينا بكتيريا متحركة + +546 +00:40:05,430 --> 00:40:11,310 +ومتزحلق، flying، كيف تزحلق flying؟ بتفرز مادة، بس + +547 +00:40:11,310 --> 00:40:15,870 +ورضه حتى إفراز المادة، إفراز المادة، تقصد لزجة، بس + +548 +00:40:15,870 --> 00:40:21,070 +مش هيك الفكرة كمان، فكرة أعقد منها، تخيل رأي + +549 +00:40:21,070 --> 00:40:25,180 +البكتيريا بهذا الشكل؟ البكتيريا قررت تروح على + +550 +00:40:25,180 --> 00:40:28,200 +اتجاهها، و تتحرك على اتجاهها اللي بتروحه هي، مفرزة زي + +551 +00:40:28,200 --> 00:40:37,440 +مُركبات...مُركبات...سالبة الشحن بكميات كبيرة في + +552 +00:40:37,440 --> 00:40:41,060 +المنطقة اللي هي موجودة فيها الآن، تمام؟ هي عالق + +553 +00:40:41,060 --> 00:40:48,190 +على الوسط تبعها، كويس؟ و في نفس الوقت بتروح تنتج + +554 +00:40:48,190 --> 00:40:52,790 +مُركبات، و بتخزنهم في مؤخرة البكتيريا...مؤخرة أو + +555 +00:40:52,790 --> 00:40:57,930 +مقدمة...ممكن تصير على عكسها...الآن negatively + +556 +00:40:57,930 --> 00:41:01,370 +charged، كمية كبيرة من المركبات، و negatively + +557 +00:41:01,370 --> 00:41:04,010 +charged مركبات أيضا مولدة داخل الخلية...إيش + +558 +00:41:04,010 --> 00:41:09,310 +حاسير؟ repulsion، هو كمان هتدفع البكتيريا اللي + +559 +00:41:09,310 --> 00:41:13,610 +أمامك، فحركت ليست بفعل فلاجلة، و إنما بفعل الـ + +560 +00:41:13,610 --> 00:41:18,950 +repulsion اللي هو التنافر ما بين الـ negatively + +561 +00:41:18,950 --> 00:41:22,750 +charged، يعني هي اللي أنطلتها و خزنتها في المنطقة + +562 +00:41:22,750 --> 00:41:26,130 +و خزنتها في نفس الشيء عندها عشان يصير في تنافر، + +563 +00:41:26,130 --> 00:41:29,590 +plasma gliding، البكتيريا طبعا العملية تتسهل أكثر + +564 +00:41:29,590 --> 00:41:32,010 +لوجود mucus أو مادة مخاطية + +565 +00:41:34,870 --> 00:41:38,490 +طبعا في قولنا gliding، و فيه non-gliding + +566 +00:41:38,490 --> 00:41:41,830 +و فيه gliding bacteria، الـ archaebacteria + +567 +00:41:41,830 --> 00:41:44,450 +أظن حكيت عنها أنا في أول المحاضرة اللي قلت هي + +568 +00:41:44,450 --> 00:41:47,410 +البكتيريا المسمى بالبكتيريا القديمة + +569 +00:41:51,250 --> 00:41:54,970 +الـ extremophile و ما أعرف احنا ميزناها عن البكتيريا + +570 +00:41:54,970 --> 00:41:59,790 +العادية ولا ميزناش بعدم وجود peptidoglycan حقيقي + +571 +00:41:59,790 --> 00:42:03,990 +يعني فيها peptidoglycan بس مش حقيقي، بمعنى إنه مش + +572 +00:42:03,990 --> 00:42:07,850 +مكون من NAM و NAG، فاكرين NAM و NAG؟ N-acetylmuramic + +573 +00:42:07,850 --> 00:42:11,810 +acid و N-acetylglucosamine، هذه كلها N- + +574 +00:42:11,810 --> 00:42:15,230 +acetylglucosamine، يعني ما فيش...ما فيش وحدات... + +575 +00:42:15,230 --> 00:42:19,960 +وحدات متعاقبة، فيه أيضا إنه cardioform actinomycetes + +576 +00:42:19,960 --> 00:42:22,860 +الـ actinomycetes أنا مرة ذكرت لكم إياها و قلت لكم إن + +577 +00:42:22,860 --> 00:42:25,200 +احنا جايين على المحاضرة كانت بالضبط كانت تشتهي + +578 +00:42:25,200 --> 00:42:29,720 +الدنيا و شمينا ريحة الشتاء، هو مش ريحة الشتاء، ريحة + +579 +00:42:29,720 --> 00:42:32,640 +الـ actinomycetes، و الـ actinomycetes من الميكروبات + +580 +00:42:32,640 --> 00:42:37,380 +العظيمة اللي قلنا عنها كمصدر للـ antibiotics في + +581 +00:42:37,380 --> 00:42:42,320 +multiocular sporangia، برضه كلمة sporangia هي مصطلح + +582 +00:42:42,320 --> 00:42:46,520 +من الفطريات جاي، و كلمة actinomycetes مش بعد actino + +583 +00:42:49,460 --> 00:42:54,780 +actino...خيوط...actino و معناها خيوط أصلا ف... + +584 +00:42:54,780 --> 00:42:58,780 +يعني...يعني النمو تبعها خيطي، و بالتالي تشبه إلى حد + +585 +00:42:58,780 --> 00:43:02,560 +كبير الفطريات، و كان في...في...في...في بداية + +586 +00:43:02,560 --> 00:43:05,840 +تصنيفها...كان في بداية تصنيفها كان تصنف بعض + +587 +00:43:05,840 --> 00:43:10,040 +الأحيان مع الفطريات، لكن لما اكتشفوا إنه إلها صفات + +588 +00:43:10,040 --> 00:43:14,670 +ال...ال...الـ prokaryotes، صنفوها مع الـ prokaryotes + +589 +00:43:14,670 --> 00:43:18,530 +multiocular sporangia، طال علي هنا هي خيرات من هنا + +590 +00:43:18,530 --> 00:43:20,990 +فاكرين ال...الـ fruiting body اللي حكينا عنه قبل + +591 +00:43:20,990 --> 00:43:25,630 +شوي؟ الـ sporangia هي organs غالبا بتكون + +592 +00:43:25,630 --> 00:43:30,330 +microscopic للفطريات تشبه الـ fruiting body، و بتحمل + +593 +00:43:30,330 --> 00:43:33,110 +على...بتحمل فيه الـ spores، الـ spores اللي هي + +594 +00:43:33,110 --> 00:43:38,410 +الأبواغ اللي...اللي...اللي بتستخدم لمين؟ للتكاثر + +595 +00:43:38,410 --> 00:43:39,610 +مش لل... + +596 +00:43:45,380 --> 00:43:49,560 +multi-echelon، يعني خلينا نقول إن في إلها أكثر من + +597 +00:43:49,560 --> 00:43:58,060 +من مكان على الخط الواحد، actinoplanetes، طبعا برضه + +598 +00:43:58,060 --> 00:44:01,920 +قريبة من الـ actinomycetes في الـ streptomyces، و الـ + +599 +00:44:01,920 --> 00:44:04,700 +streptomyces كانت تصنع في يوم من الأيام مع الـ + +600 +00:44:04,700 --> 00:44:09,520 +actinomycetes، و الـ streptomyces إذا تتأكدوا من + +601 +00:44:09,520 --> 00:44:13,990 +المعلومة، اتذكروا الـ streptomyces، الـ Streptomycin + +602 +00:44:13,990 --> 00:44:18,730 +هو antibiotic يضاهي في شهرته البنسلين، لأنه قديم + +603 +00:44:18,730 --> 00:44:22,430 +طبعا الـ antibiotics صاروا بلا شهرة الآن، لأن البكتيريا + +604 +00:44:22,430 --> 00:44:26,970 +صارت مقاومة لكثير منهم، أو كثير من البكتيريا صارت + +605 +00:44:26,970 --> 00:44:31,350 +مقاومة لهم، لكن هو من أوائل ال...ال...ال...ال... + +606 +00:44:31,350 --> 00:44:32,190 +ال...ال...ال...ال...ال...ال...ال...ال...ال + +607 +00:44:32,190 --> 00:44:33,250 +...ال...ال...ال...ال...ال...ال...ال...ال... + +608 +00:44:33,250 --> 00:44:33,790 +ال...ال...ال...ال...ال...ال...ال...ال... + +609 +00:44:33,790 --> 00:44:34,230 +...ال...ال...ال...ال...ال...ال...ال...ال... + +610 +00:44:34,230 --> 00:44:34,250 +ال...ال...ال...ال...ال...ال...ال...ال... + +611 +00:44:34,250 --> 00:44:34,290 +...ال...ال...ال...ال...ال...ال...ال...ال... + +612 +00:44:34,290 --> 00:44:34,770 +ال...ال...ال...ال...ال...ال...ال...ال... + +613 +00:44:34,770 --> 00:44:39,030 +... + +614 +00:44:40,070 --> 00:44:44,510 +في الـ micromonosporaceae برضه مجموعة من الميكروبات في + +615 +00:44:44,510 --> 00:44:48,410 +thermomonospora and related genera، thermomonospora واضح من اسمها + +616 +00:44:48,410 --> 00:44:54,210 +إنها تتحمل الحرارة، و كمان في thermoactinomycetes + +617 +00:44:54,210 --> 00:44:59,370 +برضه actinomycetes، مجموعة قدرة + +618 +00:44:59,370 --> 00:45:05,250 +خاصة على تحمل الحرارة، مجموعة تلاتة و تلاتين، اللي + +619 +00:45:05,250 --> 00:45:10,070 +ما قدرناش نصنفه في أي من الـ 32، حطينا خيار المجموعة + +620 +00:45:10,070 --> 00:45:14,490 +لحالها لحين لما نلاقي القضية، طب عارفين دي مجموعة من + +621 +00:45:14,490 --> 00:45:18,690 +ال...من الميكروبات؟ لازم أطرق لكم إياها، طيب ع + +622 +00:45:18,690 --> 00:45:20,690 +السنية Actinomycetes + +623 +00:45:23,940 --> 00:45:27,040 +خدوا بالكم هذا عبارة عن خلطة من الميكروبات، مش نوع + +624 +00:45:27,040 --> 00:45:31,180 +واحد، وإنما to make the names of microorganisms + +625 +00:45:31,180 --> 00:45:37,600 +familiar to you، كمرة مش للحفظ وإنما لجلها يعني + +626 +00:45:37,600 --> 00:45:44,420 +ملوفة لديكم باسيلاس، بوداتيلا، بوريليا، بروسيلا، + +627 +00:45:44,420 --> 00:45:50,640 +كاليسي فايروس، كامبيلو بارتر، كانديدا، كلاميديا، + +628 +00:45:50,640 --> 00:45:51,360 +كلستريديوم + +629 +00:45:54,670 --> 00:45:59,510 +Cryptococcus, Enterovirus, Escherichia طبعا هذه + +630 +00:45:59,510 --> 00:46:04,590 +المشهورة ساعدة انكوا Flavivirus ما فيش داعي انه كل + +631 +00:46:04,590 --> 00:46:10,650 +مرة نقول Flavivirus هو فيروس من أما بينتهي ب .. + +632 +00:46:10,650 --> 00:46:13,650 +Hemophilus مكتوبة كانت انكوا خاطئة بالمحاضرة اللي + +633 +00:46:13,650 --> 00:46:17,110 +نزلتها عدلناها Hemophilus Hepatitis viruses + +634 +00:46:17,110 --> 00:46:19,290 +Helicobacter pylori مش Helicobacter + +635 +00:46:22,650 --> 00:46:26,030 +Helicobacter pylori أي واحد عنده قرحة معينية يعرفه أنه هي + +636 +00:46:26,030 --> 00:46:31,150 +المسبب لهذه القرحة Helicobacter pylori Hemorrhagic fever + +637 +00:46:31,150 --> 00:46:35,970 +virus Herpes viruses Legionella Leptospira + +638 +00:46:35,970 --> 00:46:41,190 +Mycobacterium Mycoplasma أسماء صارت في جزء منها + +639 +00:46:41,190 --> 00:46:44,750 +familiar بالفعل Neisseria Nocardia + +640 +00:46:44,750 --> 00:46:48,770 +Orthomyxoviruses اللي بتشمل ال influenza virus + +641 +00:46:49,870 --> 00:46:54,670 +Papillomavirus Paramyxovirus لاحظوا الأسماء اللي + +642 +00:46:54,670 --> 00:46:58,750 +طويلة ما منعش يعني قدرة على لفظها مرة واحدة + +643 +00:46:58,750 --> 00:47:03,530 +بنجبطها Paramyxovirus Orthomyxoviruses + +644 +00:47:03,530 --> 00:47:07,050 +Parvoviruses + +645 +00:47:07,050 --> 00:47:10,010 +Prions ال Prions هحطوا تحتها خط ونكلم عنها بعدين + +646 +00:47:10,010 --> 00:47:14,950 +Pseudomonas Rabies virus Rubella virus + +647 +00:47:14,950 --> 00:47:16,470 +Retroviruses + +648 +00:47:18,290 --> 00:47:21,250 +Rhinoviruses ريكيزيا مرض معانا ريكيزيا اللي سيجيب + +649 +00:47:21,250 --> 00:47:25,890 +البقايا Rotavirus Salmonella Shigella + +650 +00:47:25,890 --> 00:47:30,170 +Staphylococcus أو Staphylococci Streptococci + +651 +00:47:30,170 --> 00:47:33,030 +Treponema مرض معانا ال spiral اللي رسلناها على + +652 +00:47:33,030 --> 00:47:38,110 +الصطبورة هذه Treponema pallidumاليوريا بلازما و ال + +653 +00:47:38,110 --> 00:47:41,530 +Vibrio و الرسمية طبعا انا بقولكوا يعني بالبداية + +654 +00:47:41,530 --> 00:47:45,730 +يمكن يكون في صعوبة في نطق الكلمات لكن بعد مرة او + +655 +00:47:45,730 --> 00:47:49,250 +مرتين بتصير يعني محببة و مقفلة diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipIvFNbRCx0_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipIvFNbRCx0_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..e9ccce06f0f4f69e8047dd017481958b9dd0edb5 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipIvFNbRCx0_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 4894, "start": 21.72, "end": 48.94, "text": "بسم الله الرحمن الرحيم بالبدأ بقولكوا كل عامة وبخير عودة من الإجازة يعني بيكون الإنسان شوية كسول فاعطونا يوم الأربع عشان نتنشط ليوم السبت إن شاء كنا بدأنا الحديث 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{"start": 306.72, "end": 307.06, "word": " على", "probability": 0.93896484375}, {"start": 307.06, "end": 307.78, "word": " solid", "probability": 0.8134765625}, {"start": 307.78, "end": 308.28, "word": " medium", "probability": 0.68017578125}, {"start": 308.28, "end": 310.98, "word": " قدامكوا", "probability": 0.77158203125}, {"start": 310.98, "end": 311.34, "word": " لوحة", "probability": 0.9801432291666666}, {"start": 311.34, "end": 311.68, "word": " بتتكلم", "probability": 0.921142578125}, {"start": 311.68, "end": 312.2, "word": " عن", "probability": 0.99462890625}, {"start": 312.2, "end": 312.4, "word": " ال", "probability": 0.919921875}, {"start": 312.4, "end": 313.14, "word": " semi", "probability": 0.28955078125}, {"start": 313.14, "end": 313.48, "word": "-solid", "probability": 0.7941080729166666}, {"start": 313.48, "end": 313.82, "word": " medium", "probability": 0.88623046875}, {"start": 313.82, "end": 314.72, "word": " ال", "probability": 0.33056640625}, {"start": 314.72, "end": 314.94, "word": " semi", "probability": 0.86767578125}, {"start": 314.94, "end": 315.42, "word": "-solid", "probability": 0.8981119791666666}, {"start": 315.42, "end": 317.4, "word": " غرض", "probability": 0.642333984375}, {"start": 317.4, "end": 318.12, "word": " أساسى", "probability": 0.8822021484375}, {"start": 318.12, "end": 318.56, "word": " إلها", "probability": 0.7156575520833334}, {"start": 318.56, "end": 318.84, "word": " to", "probability": 0.8701171875}, {"start": 318.84, "end": 319.2, "word": " detect", "probability": 0.962890625}, {"start": 319.2, "end": 319.48, "word": " the", "probability": 0.91357421875}, {"start": 319.48, "end": 319.92, "word": " motility", "probability": 0.947021484375}, {"start": 319.92, "end": 320.14, "word": " of", "probability": 0.98388671875}, {"start": 320.14, "end": 320.62, "word": " bacteria", "probability": 0.76123046875}], "temperature": 1.0}, {"id": 14, "seek": 34966, "start": 321.32, "end": 349.66, "text": "فاكريني لما اتكلمنا عن ال flagella و أن البكتيريا بتتحرك بال flagella سباحة ولا طيران ولا ماشي تسبح و السباحة بتكون في إيه؟ في سائل أنا لسه قبل دقيقة بقول إنه البكتيريا الغير متحركة قادرة على الانتقال في السائل من مكان إلى مكان بذال حركة دذائقات في السائل إذا كيف بتقعد من البكتيريا متحركة أو غير متحركة؟ ع الصلب بتمشيش", "tokens": [5172, 995, 37983, 9957, 1829, 5296, 15042, 1975, 2655, 4117, 19528, 8315, 18871, 2423, 932, 609, 3505, 4032, 14739, 29739, 4117, 2655, 13546, 25528, 39894, 2655, 5016, 31747, 20666, 932, 609, 3505, 8608, 3555, 39319, 3660, 49429, 23032, 13546, 7649, 49429, 3714, 33599, 1829, 6055, 35457, 5016, 4032, 21136, 3555, 39319, 3660, 39894, 30544, 8978, 11933, 1829, 3224, 22807, 8978, 8608, 16373, 1211, 41850, 5296, 3794, 3224, 12174, 36150, 11778, 38436, 28671, 4724, 39648, 36145, 3224, 29739, 4117, 2655, 13546, 25528, 6024, 118, 13546, 44650, 5016, 31747, 3660, 12174, 18513, 25720, 15844, 2423, 7649, 2655, 4587, 6027, 8978, 21136, 16373, 1211, 9154, 3714, 41361, 30731, 3714, 41361, 4724, 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323.92, "word": " أن", "probability": 0.424072265625}, {"start": 323.92, "end": 324.56, "word": " البكتيريا", "probability": 0.9505859375}, {"start": 324.56, "end": 325.18, "word": " بتتحرك", "probability": 0.8714599609375}, {"start": 325.18, "end": 325.36, "word": " بال", "probability": 0.85595703125}, {"start": 325.36, "end": 326.0, "word": " flagella", "probability": 0.919921875}, {"start": 326.0, "end": 327.08, "word": " سباحة", "probability": 0.9031982421875}, {"start": 327.08, "end": 327.8, "word": " ولا", "probability": 0.822265625}, {"start": 327.8, "end": 328.5, "word": " طيران", "probability": 0.94775390625}, {"start": 328.5, "end": 328.84, "word": " ولا", "probability": 0.88720703125}, {"start": 328.84, "end": 329.24, "word": " ماشي", "probability": 0.7835286458333334}, {"start": 329.24, "end": 331.24, "word": " تسبح", "probability": 0.7445475260416666}, {"start": 331.24, "end": 331.54, "word": " و", "probability": 0.39306640625}, {"start": 331.54, "end": 331.9, "word": " 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"end": 343.12, "word": " حركة", "probability": 0.9309895833333334}, {"start": 343.12, "end": 343.76, "word": " دذائقات", "probability": 0.688427734375}, {"start": 343.76, "end": 344.02, "word": " في", "probability": 0.63818359375}, {"start": 344.02, "end": 344.66, "word": " السائل", "probability": 0.9832356770833334}, {"start": 344.66, "end": 345.4, "word": " إذا", "probability": 0.75244140625}, {"start": 345.4, "end": 345.58, "word": " كيف", "probability": 0.886474609375}, {"start": 345.58, "end": 345.88, "word": " بتقعد", "probability": 0.8434244791666666}, {"start": 345.88, "end": 345.96, "word": " من", "probability": 0.7607421875}, {"start": 345.96, "end": 346.34, "word": " البكتيريا", "probability": 0.98740234375}, {"start": 346.34, "end": 346.96, "word": " متحركة", "probability": 0.992431640625}, {"start": 346.96, "end": 347.06, "word": " أو", "probability": 0.61865234375}, {"start": 347.06, "end": 347.26, "word": " غير", "probability": 0.91162109375}, {"start": 347.26, "end": 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350.34, "end": 350.46, "word": " ال", "probability": 0.92724609375}, {"start": 350.46, "end": 350.84, "word": " liquid", "probability": 0.7939453125}, {"start": 350.84, "end": 352.02, "word": " هتختلط", "probability": 0.821923828125}, {"start": 352.02, "end": 352.58, "word": " الحابل", "probability": 0.8092447916666666}, {"start": 352.58, "end": 352.94, "word": " منها", "probability": 0.805419921875}, {"start": 352.94, "end": 353.3, "word": " بنظرا", "probability": 0.5530598958333334}, {"start": 353.3, "end": 353.72, "word": " ليه", "probability": 0.92431640625}, {"start": 353.72, "end": 354.42, "word": " حركة", "probability": 0.9456380208333334}, {"start": 354.42, "end": 354.68, "word": " وجهة", "probability": 0.4432779947916667}, {"start": 354.68, "end": 355.16, "word": " السائل", "probability": 0.8932291666666666}, {"start": 355.16, "end": 356.08, "word": " فصممنا", "probability": 0.93515625}, {"start": 356.08, "end": 356.48, "word": " وسط", "probability": 0.94873046875}, {"start": 356.48, "end": 356.86, "word": " ما", "probability": 0.95556640625}, {"start": 356.86, "end": 357.44, "word": " بينهما", "probability": 0.8375651041666666}, {"start": 357.44, "end": 357.56, "word": " اللي", "probability": 0.826904296875}, {"start": 357.56, "end": 357.66, "word": " هو", "probability": 0.9736328125}, {"start": 357.66, "end": 357.76, "word": " ال", "probability": 0.732421875}, {"start": 357.76, "end": 357.94, "word": " semi", "probability": 0.51513671875}, {"start": 357.94, "end": 358.26, "word": "-solid", "probability": 0.740234375}, {"start": 358.26, "end": 358.62, "word": " media", "probability": 0.9580078125}, {"start": 358.62, "end": 359.26, "word": " طلعوا", "probability": 0.9061279296875}, {"start": 359.26, "end": 359.38, "word": " على", "probability": 0.82861328125}, {"start": 359.38, "end": 359.76, "word": " الأنبوبة", "probability": 0.8962890625}, {"start": 359.76, "end": 359.94, "word": " ها", "probability": 0.5191650390625}, {"start": 359.94, "end": 360.06, "word": " دي", "probability": 0.7080078125}, {"start": 360.06, "end": 360.3, "word": " اللي", "probability": 0.96875}, {"start": 360.3, "end": 360.6, "word": " هي", "probability": 0.61279296875}, {"start": 360.6, "end": 361.18, "word": " أمامنا", "probability": 0.9263916015625}, {"start": 361.18, "end": 361.5, "word": " الآن", "probability": 0.726318359375}, {"start": 361.5, "end": 361.74, "word": " اللي", "probability": 0.970458984375}, {"start": 361.74, "end": 361.98, "word": " باللون", "probability": 0.8541666666666666}, {"start": 361.98, "end": 362.68, "word": " الأحمرها", "probability": 0.873291015625}, {"start": 362.68, "end": 363.34, "word": " زرعنا", "probability": 0.9139404296875}, {"start": 363.34, "end": 363.76, "word": " البكتيريا", "probability": 0.9166015625}, {"start": 363.76, "end": 364.04, "word": " بشكل", "probability": 0.9293619791666666}, {"start": 364.04, "end": 364.46, "word": " طعنة", "probability": 0.920654296875}, {"start": 364.46, "end": 364.8, "word": " step", "probability": 0.19873046875}, {"start": 364.8, "end": 366.3, "word": " بعد", "probability": 0.90380859375}, {"start": 366.3, "end": 366.46, "word": " ما", "probability": 0.78759765625}, {"start": 366.46, "end": 366.64, "word": " نمت", "probability": 0.9820963541666666}, {"start": 366.64, "end": 367.34, "word": " البكتيريا", "probability": 0.98740234375}, {"start": 367.34, "end": 367.76, "word": " نمت", "probability": 0.9703776041666666}, {"start": 367.76, "end": 367.86, "word": " في", "probability": 0.95654296875}, {"start": 367.86, "end": 368.06, "word": " نفس", "probability": 0.998291015625}, {"start": 368.06, "end": 368.38, "word": " مكان", "probability": 0.991455078125}, {"start": 368.38, "end": 368.78, "word": " الطعنة", "probability": 0.9837646484375}, {"start": 368.78, "end": 369.04, "word": " دلوقت", "probability": 0.64227294921875}, {"start": 369.04, "end": 369.16, "word": " على", "probability": 0.285400390625}, {"start": 369.16, "end": 369.52, "word": " أنه", 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اللي.. + +5 +00:00:35,910 --> 00:00:39,350 +اللي بتغير ألوانها في المنطقة هذه حاجة بيسموها + +6 +00:00:39,350 --> 00:00:41,810 +chain of transmission إيش هي الـ chain of + +7 +00:00:41,810 --> 00:00:45,450 +transmission؟ سلسلة الانتقال، لو بينتقل الميكروب + +8 +00:00:45,450 --> 00:00:51,390 +من إنسان إلى إنسان، من حيوان إلى إنسان، تمام؟ في + +9 +00:00:51,390 --> 00:00:55,930 +سلسلة من.. أو خطوات معينة لازم تحدث، يجب أن تحدث + +10 +00:00:57,020 --> 00:01:00,680 +هذه الخطوات بنسميها chain of transmission فالـصورة + +11 +00:01:00,680 --> 00:01:03,740 +اللي قدامكم ممكن تلاقوها بشكل سلس في الـ internet + +12 +00:01:03,740 --> 00:01:06,960 +أو في أماكن أخرى لكن في الآخر بتعبر عن نفس المعنى + +13 +00:01:06,960 --> 00:01:10,880 +بتعبر عن نفس المعنى ليش سميناها سلسلة؟ لأن لو + +14 +00:01:10,880 --> 00:01:14,540 +بيصير فيها السلسلة في مكان احنا بنقطع الـ chain of + +15 +00:01:14,540 --> 00:01:18,950 +transmission وبالتالي احنا من وجبنا لما نعمل + +16 +00:01:18,950 --> 00:01:22,810 +control للـ micro organism أو للـ infection أنه نفهم + +17 +00:01:22,810 --> 00:01:27,650 +الـ infection cycle أو الـ chain of transmission فهم + +18 +00:01:27,650 --> 00:01:33,150 +معمق بحيث أنه نفكر كيف ممكن نقطع هذا الـ cycle في + +19 +00:01:33,150 --> 00:01:35,830 +أي جانب أنه الأسهل أنه الأرخص أنه الأضمن أنه + +20 +00:01:35,830 --> 00:01:39,650 +الأسرع كل هذه النقاط ممكن نفهمها إن شاء الله من + +21 +00:01:39,650 --> 00:01:44,570 +هذه المحاضرة الـصورة الثانية معبرة عن عين وفيروسات + +22 +00:01:45,900 --> 00:01:49,500 +الفيروسات اللي من ضمنها الـ influenza اللي من ضمنها + +23 +00:01:49,500 --> 00:01:52,580 +الرشح أنتو كلكم متخيلين أنها بتنتقل قصاد مين؟ الـ + +24 +00:01:52,580 --> 00:01:58,080 +respiratory tract فقط، لكن ممكن finger to eye، إيش + +25 +00:01:58,080 --> 00:02:03,570 +يعني finger to eye؟ يعني واحد.. راكز.. فأسطح واحد + +26 +00:02:03,570 --> 00:02:06,950 +جنبه كان عاطس وحاطط الفيروس على الطاولة واحنا + +27 +00:02:06,950 --> 00:02:10,330 +لمسناه وبعد شوية أجى عنده شعور بالحكة ودخل إصبعه + +28 +00:02:10,330 --> 00:02:15,970 +في عينه نقل الفيروس من الـ surface إلى الـ .. الـ .. + +29 +00:02:15,970 --> 00:02:18,450 +الـ .. الـ nucleus membrane في العين وبالتالي يمكن + +30 +00:02:18,450 --> 00:02:22,370 +يعمل infection إذا هي source مهمة لمين؟ للـ infection + +31 +00:02:24,680 --> 00:02:29,240 +في العلاقات ما بين أكثر من مخلوقين أو مخلوقين أو + +32 +00:02:29,240 --> 00:02:33,040 +أكثر قد تنقسم العلاقات إلى ثلاث أشكال إما + +33 +00:02:33,040 --> 00:02:36,560 +commensalism أو parasitism أو mutualism كلكم + +34 +00:02:36,560 --> 00:02:40,660 +درستم في أحياء ألف ودرستم في ثانوية وقبل ذلك + +35 +00:02:40,660 --> 00:02:46,280 +لكن اللي أنا بركز عليها في الـ medical microbiology + +36 +00:02:46,280 --> 00:02:46,860 +علاقة + +37 +00:02:49,640 --> 00:02:53,500 +الـ Parasitism ليش؟ لأنه فيه effect، harmful + +38 +00:02:53,500 --> 00:02:57,080 +effect، واحد بيستفيد اللي هو الـ pathogen الـ + +39 +00:02:57,080 --> 00:03:00,360 +parasite وواحد بينضر اللي هو الـ host اللي هو + +40 +00:03:00,360 --> 00:03:02,960 +الإنسان في الحالة وابتدا الـ medical microbiome قد + +41 +00:03:02,960 --> 00:03:08,500 +اضطر بشيء من الذكر لـ commensals اللي هي + +42 +00:03:08,500 --> 00:03:14,470 +بتستفيد هي والجسم بندرش تمام؟ وقد نتطرق إلى الـ + +43 +00:03:14,470 --> 00:03:17,650 +mutualism زي الـ normal flora احنا بنوفرلها مكان وهي + +44 +00:03:17,650 --> 00:03:21,550 +بتعطينا vitamin K بنوفرلها مكان وهي بتساعدنا + +45 +00:03:21,550 --> 00:03:25,570 +في الحماية من infection من others لكن في الغالب + +46 +00:03:25,570 --> 00:03:28,930 +تركيزنا هيكون على أي واحدة منهم؟ على اللي هي الـ + +47 +00:03:28,930 --> 00:03:35,010 +parasitism طيب مدام سبب نتكلمين على الـ normal flora + +48 +00:03:35,010 --> 00:03:38,210 +كلمة flora بنات معناها باللغة العربية نباتات فلما + +49 +00:03:38,210 --> 00:03:41,510 +بيقول flora أو فلسطين وبمكاننا بيقول نباتات + +50 +00:03:41,510 --> 00:03:42,170 +فلسطين + +51 +00:03:47,960 --> 00:03:54,600 +الإنسان بشكل دائم هو الاسم القديم normal flora + +52 +00:03:54,600 --> 00:04:00,880 +طبعا لأنه زمان كانت تدرس البكتيريا تحت علم البطن + +53 +00:04:00,880 --> 00:04:07,200 +يعني علم النبات لأنهم كانوا صنفوها أيضا نبات فاتطلق + +54 +00:04:07,200 --> 00:04:10,120 +أي لقب flora لكن الآن وبعد ما احنا خلاص يعني صارت + +55 +00:04:10,120 --> 00:04:14,740 +إلى مملكة لحالها تمام صار من الجدير أنه أو من + +56 +00:04:14,740 --> 00:04:17,360 +الأشدر أن احنا نسميها اسم خاص فيها فسموها + +57 +00:04:17,360 --> 00:04:21,900 +microbiota فسميناها سواء اسمته normal flora أو normal + +58 +00:04:21,900 --> 00:04:26,560 +microbiota المعنى هو واحد من فوائد جبل من الفوائد + +59 +00:04:26,560 --> 00:04:29,160 +أنا قلت إن الجسم بنقسم إلى قسمين مرة ده بتذكروا + +60 +00:04:29,160 --> 00:04:32,680 +بنقسم إلى sterile و non sterile الـ non sterile + +61 +00:04:32,680 --> 00:04:37,080 +بالتأكيد بحتوي على normal flora والـ normal flora + +62 +00:04:37,080 --> 00:04:41,040 +إلها فوائد عظيمة، يعني مش موجودة هيك بس عبثا، لأ + +63 +00:04:41,040 --> 00:04:45,860 +إلها فوائد عظيمة وكثيرة، من أهم فوائدها، بديش + +64 +00:04:45,860 --> 00:04:48,980 +أقول رقم واحد، رقم اثنين، رقم اثنين هي من أهم + +65 +00:04:48,980 --> 00:04:52,780 +فوائدها، حاجة بيسميها colonization resistance، + +66 +00:04:52,780 --> 00:04:56,740 +بالعربي مقاومة الاستعمار، يعني مش لحاجة أهل فلسطين + +67 +00:04:56,740 --> 00:04:59,700 +بتقاوم الاستعمار، كمان فينا اللي هو الـ normal + +68 +00:04:59,700 --> 00:05:03,160 +microbiota، ما معناه مقاومة الاستعمار بهذا الوجه + +69 +00:05:03,160 --> 00:05:10,570 +المفهوم تبقى تخيلوا أنكم كلكم من الأصدقاء، تمام؟ وأنكم + +70 +00:05:10,570 --> 00:05:14,870 +أبين الكراسي اللي في القاعة دي كلها، لو يجي + +71 +00:05:14,870 --> 00:05:17,630 +واحد من الأعداء بدي يحطله موطئ قدم، بيلاجي مكان + +72 +00:05:17,630 --> 00:05:21,950 +يقعد فيه، يبقى وجودكم في هذا المكان فقط وجودكم، + +73 +00:05:21,950 --> 00:05:27,050 +تمام؟ محتلين مواقع معينة في هذا المكان مانع آخرين + +74 +00:05:27,050 --> 00:05:32,330 +من أنهم يستوطنوا ويستعمروا كولونايزايشن وأول + +75 +00:05:32,330 --> 00:05:36,330 +خطوة في الـ infection كولونايزايشن وأول خطوة في + +76 +00:05:36,330 --> 00:05:40,630 +الـ كولونايزايشن attachment مانجلاش مكان نقعد فيه + +77 +00:05:40,630 --> 00:05:46,650 +تمام الـ pathogen مع السلامة مش بس هيك لو كان فينا + +78 +00:05:46,650 --> 00:05:51,650 +pathogen كمان وجايب معاه عدد كبيرة يعني ممكن + +79 +00:05:51,650 --> 00:05:57,320 +ينافسوا فاكرين لما درسنا منتجات البكتيريا اللي من + +80 +00:05:57,320 --> 00:06:00,520 +ضمنها الـ toxins درسنا منتج اسمه bactericin رقم + +81 +00:06:00,520 --> 00:06:08,680 +أربعة رقم ثلاثة bactericin اللي هي antibiotic + +82 +00:06:08,680 --> 00:06:12,660 +-like سميناها antibiotic-like compounds produced + +83 +00:06:12,660 --> 00:06:12,940 +by + +84 +00:06:16,130 --> 00:06:21,010 +كده من المنافسة، كمان هذا بيساعد على أنه نمنع الـ + +85 +00:06:21,010 --> 00:06:24,130 +colonization، إذا في عندي رقم اثنين وثلاثة + +86 +00:06:24,130 --> 00:06:29,890 +بيساهموا في منع الأمراض، في منع حدوث الأمراض، ليش؟ + +87 +00:06:29,890 --> 00:06:34,190 +بيمنعوا استيطان أو استعمار اللي هم الـ pathogenic + +88 +00:06:34,190 --> 00:06:38,290 +microorganism رقم واحد بعض البكتيريا بتنتج vitamin + +89 +00:06:38,290 --> 00:06:42,970 +K و vitamin K جيد للإنسان بيستفيد منه رقم أربعة + +90 +00:06:42,970 --> 00:06:47,370 +بقول إن الـ normal flora قد تساهم في الـ stimulation + +91 +00:06:47,370 --> 00:06:52,390 +لـ special organs أو special tissue في مجموعة من الـ + +92 +00:06:52,390 --> 00:06:57,670 +lymph nodes موجودة في الأمعاء اسمها biopatches وهي + +93 +00:06:57,670 --> 00:07:02,650 +specialized lymph node موجودين في الأمعاء إيش + +94 +00:07:02,650 --> 00:07:08,870 +الـ lymph nodes؟ بيوصفوها في علم المناعة أنها the + +95 +00:07:08,870 --> 00:07:13,030 +budget check points نقاط التفتيش تبع الجسم إيش هي + +96 +00:07:13,030 --> 00:07:18,390 +نقاط التفتيش؟ تخيلوا إن القاعة هذه مخصصة لطالبات + +97 +00:07:18,390 --> 00:07:21,610 +قسم العلم الطبي المخبرية سنة ثانية ممنوع يدخلها + +98 +00:07:21,610 --> 00:07:24,990 +سنة ثالثة ممنوع يدخلها سنة أولى فلازم يكون على + +99 +00:07:24,990 --> 00:07:30,730 +البا حد يعمل check صح؟ على البطاقة أنه فقط طالبات + +100 +00:07:30,730 --> 00:07:34,190 +سنة ثانية مسموح لهم فالـ .. الـ .. الـ .. الـ .. الـ + +101 +00:07:34,190 --> 00:07:37,180 +..الـ Lymph node اللي موجودة في الـ bio patches + +102 +00:07:37,180 --> 00:07:41,800 +بتكون بدور عظيم جدا في مناعة العدوى وخاصة أنا + +103 +00:07:41,800 --> 00:07:44,920 +هنتكلم عن الـ intestinal tract مليارات وملايين من + +104 +00:07:44,920 --> 00:07:49,640 +الأنواع من البكتيريا وبالتالي هي نوع الـ microbiome + +105 +00:07:49,640 --> 00:07:53,880 +عملت stimulation لهذا النسيج يعني خلتة دائما اه + +106 +00:07:53,880 --> 00:07:56,800 +متحفز وساعد في الـ development تبعه + +107 +00:08:04,260 --> 00:08:07,620 +colonization resistance ذكرتها في الأمعاء لكن + +108 +00:08:07,620 --> 00:08:11,700 +تذكروا الأنثى كمان إلها خاصية الفم الفاجاينة + +109 +00:08:11,700 --> 00:08:14,820 +فيها normal microbiota وبتعمل colonization + +110 +00:08:14,820 --> 00:08:19,300 +resistance الستات الحوامل بيصير عندهم urinary + +111 +00:08:19,300 --> 00:08:22,000 +tract infection أو vagina infection أكثر من اللي + +112 +00:08:22,000 --> 00:08:25,560 +مش حوامل عارفين ليش؟ لأن الـ hormonal changes اللي + +113 +00:08:25,560 --> 00:08:29,920 +بتصير عندها بتأثر على الـ normal flora وبالتالي + +114 +00:08:29,920 --> 00:08:33,950 +بيصير الـ normal flora مش طبيعية بيصير الـ توازن تبعها + +115 +00:08:33,950 --> 00:08:38,210 +في خلال فبيصير فينا إمكانية colonization لـ other + +116 +00:08:38,210 --> 00:08:43,690 +microorganisms هحاكيلكم كمان نقطة كتير مهمة + +117 +00:08:43,690 --> 00:08:51,970 +concept بدنا نفهمها كويس لو احنا اثنين شخصين بدنا + +118 +00:08:51,970 --> 00:08:56,430 +نروح على الغرف الثانية وبدنا نعمله مشكلة مشكلة + +119 +00:08:56,430 --> 00:08:59,760 +لكوا كده إيش البنات اللي قدامهم خمسين بنت راحوا + +120 +00:08:59,760 --> 00:09:02,660 +البنتين هؤلاء المسكين كل واحدة معاها جلم ولا سكينة + +121 +00:09:02,660 --> 00:09:06,680 +ولا حاجة ورفعت بإن أنا أقتلكوا 150 إبنة، ليش + +122 +00:09:06,680 --> 00:09:09,900 +بيكونوا عاملين واقع لعمد؟ لأ لأ مش بيصيروا يضحكوا، + +123 +00:09:09,900 --> 00:09:15,520 +جانوا على أنفسهم، هم أعلنوا عن عدوتهم تمام؟ وهم لا + +124 +00:09:15,520 --> 00:09:18,580 +يمتلكوا من القوة شيء، تعرفوا إن البكتيريا أذكى + +125 +00:09:18,580 --> 00:09:23,480 +منكم، من البنتين جزء اللي راحوا يعني البكتيريا لن + +126 +00:09:23,480 --> 00:09:26,640 +تقلل عن نفسها .. وأقول لكم ال analogy تبعها + +127 +00:09:26,640 --> 00:09:30,180 +للبكتيريا، لو خليتين بكتيريتين قرروا أنهم .. دخلوا + +128 +00:09:30,180 --> 00:09:32,820 +الدسيل، وقرروا أنهم ينطلوا التوكسين، يعني رفعوا + +129 +00:09:32,820 --> 00:09:38,720 +السلاح تبعهم، التوكسين لن يكون له أي تأثير، لأن + +130 +00:09:38,720 --> 00:09:43,240 +كميته إيش هتكون؟ من two cells، قليل جدا، لكن هيكون + +131 +00:09:43,240 --> 00:09:46,020 +له أثر أنهم رفعوا السكين، بمعنى آخر جاز المناعة + +132 +00:09:46,020 --> 00:09:52,440 +تنبه لهم، فقتلوا، يعني أعلنوا عن نفسهم مبكرا كان سيئ + +133 +00:09:52,440 --> 00:09:57,060 +سبب في موتهم، فالبكتيريا ذكية، البكتيريا لن تعلن عن + +134 +00:09:57,060 --> 00:10:01,400 +نفسها إلا إذا وصل عددها، يعني معنى الحكي كلمة وصل + +135 +00:10:01,400 --> 00:10:06,860 +عددها، يعني معاها تقنية، عندها تقنية تعرف فيها + +136 +00:10:06,860 --> 00:10:09,560 +من خلالها الأدب تبعها، أنتم ما عندكم التقنية هذه + +137 +00:10:09,560 --> 00:10:12,940 +يعني لو غمينا واحدة منكم إحنا، وقلنا للطالبات ادخلوا + +138 +00:10:12,940 --> 00:10:16,220 +الصف يلا، بنت، أنتِ اللي مغمى عليكِ، أكمل واحدة في الصف مش + +139 +00:10:16,220 --> 00:10:20,040 +هتعرفوا، بس البكتيريا بتعرفوا، الظاهرة بنسميها Quorum + +140 +00:10:20,040 --> 00:10:25,640 +Sensing، Quorum Sensing، إحساس بالعدد الكلي، Quorum + +141 +00:10:25,640 --> 00:10:30,440 +Sensing، طبعا حاجة وقت نشرحها بتفاصيل أكثر كيف بتتم + +142 +00:10:30,440 --> 00:10:33,860 +لكن بنلخصها من ناحية النقطة إيش .. إيش الدلالة اللي أنا + +143 +00:10:33,860 --> 00:10:38,440 +بدي أوصلها في هذا الموضوع، الـ normal microbiota + +144 +00:10:38,440 --> 00:10:44,660 +موجودة بأنواع مختلفة في مكان واحد، أنواع مختلفة + +145 +00:10:44,660 --> 00:10:50,440 +بأعداد محددة، بأعداد محددة، هذه الأعداد المحددة غير + +146 +00:10:50,440 --> 00:10:56,360 +كافية للإعلان عن نفسها، لإنتاج virulence factors + +147 +00:10:56,360 --> 00:11:03,200 +عوامل الشراسة اللي من ضمنها الـ toxins، نفترض الشجهة + +148 +00:11:03,200 --> 00:11:09,680 +هذه هي المجموعة اللي عاجلة وممتازة وجاينة دور + +149 +00:11:09,680 --> 00:11:15,770 +كويس، لأن عددهم قليل، جبنا دموع ورشينا عليكم أنتم + +150 +00:11:15,770 --> 00:11:18,350 +المجموعة الكبرى اللي كانت مسيطرة على المجموعة، دموع + +151 +00:11:18,350 --> 00:11:23,820 +مانعاها أنها تكبر وموتنا، الآن الغذاء اللي كان مخصص + +152 +00:11:23,820 --> 00:11:27,820 +لكم، المساحة صارت فاضية، مين هيستغلها؟ هيستغلها عددهم + +153 +00:11:27,820 --> 00:11:31,920 +ضعف أو أضعاف، عندهم الأول، إذا وصل العدد لـ quorum + +154 +00:11:31,920 --> 00:11:35,060 +sensing، بيبطلوا عاجلين وكويسين، إيش بيصيروا الآن؟ + +155 +00:11:35,060 --> 00:11:40,100 +ينتجوا الـ virulence factors اللي من ضمنها الـ toxin + +156 +00:11:40,100 --> 00:11:45,240 +لذلك هناك أمراض قد تنشأ من وجود خلل في الـ normal + +157 +00:11:45,240 --> 00:11:49,860 +microbiota، وصلت الصورة؟ بعدين هم normal لما بيكونوا + +158 +00:11:49,860 --> 00:11:53,700 +في مكانهم، normal، لما بيطلع من مكانهم إلى مكان آخر + +159 +00:11:53,700 --> 00:11:58,860 +بيبطل normal، يعني أعطيكم مثال واضح وصريح، الشريشيا + +160 +00:11:58,860 --> 00:12:02,820 +كولاي is a normal flora of the intestinal tract + +161 +00:12:02,820 --> 00:12:06,640 +الشريشيا + +162 +00:12:06,640 --> 00:12:09,200 +كولاي is number one cause of urinary tract + +163 +00:12:09,200 --> 00:12:14,320 +infection، يبقى لما انتقلت من الـ intestine إلى الـ + +164 +00:12:14,320 --> 00:12:18,360 +urinary tract عملت infection، يبقى مش كلهم normal + +165 +00:12:18,360 --> 00:12:20,900 +flora، يعني normal flora يعني مصطلح يطلق عليهم + +166 +00:12:20,900 --> 00:12:24,220 +normal flora، لأ، في مكانه هو normal flora، وربما + +167 +00:12:24,220 --> 00:12:30,060 +يكون العدد كمان عامل محدد مهم، وبما أن إحنا ذكرنا + +168 +00:12:30,060 --> 00:12:34,260 +كلمة عدد، تعالوا نتكلم على إيش العوامل الثلاثة أصلا + +169 +00:12:34,260 --> 00:12:40,340 +اللي بتساهم في الـ infection، الـ host immunity، الـ + +170 +00:12:40,340 --> 00:12:48,750 +type of microorganism، وكلمة type تشير لشراسته، والـ + +171 +00:12:48,750 --> 00:12:52,170 +number اللي دخل، الـ number تبع الـ microorganism هاي + +172 +00:12:52,170 --> 00:12:57,970 +ثلاث عوامل، كمان مرة، الـ host immunity، الـ type، وكنا + +173 +00:12:57,970 --> 00:13:00,690 +كاملة الـ type والـ organism، كنا إشارة لمن؟ + +174 +00:13:00,690 --> 00:13:06,250 +للـ virulence، رقم ثلاثة الـ number، في ضعفات أخرى لكن هي + +175 +00:13:06,250 --> 00:13:10,750 +أهم الثلاث عوامل اللي بتساهم أنه يصير infection، و + +176 +00:13:10,750 --> 00:13:12,690 +لا ما يصير إيش infection، وأعطيكم إشارة بسيطة عشان + +177 +00:13:12,690 --> 00:13:18,930 +نفسنا أن الشيجيلوزا شجلة تمام، حتى عند الإنسان الصحيح + +178 +00:13:18,930 --> 00:13:22,290 +الإنسان اللي عنده جهاز مناعي كويس، الـ number + +179 +00:13:22,290 --> 00:13:27,840 +required، الشجلة number شوية، من one to ten cells + +180 +00:13:27,840 --> 00:13:34,000 +خلية لعشر خلايا، في المقابل لو الـ type اختلف من + +181 +00:13:34,000 --> 00:13:38,120 +Shigella لـ Vibrio، محتاج لمليون، محتاج لمليون إيش؟ + +182 +00:13:38,120 --> 00:13:43,540 +العامل اللي اختلف، نوع البكتيريا، فائدة الاختلاف اللي + +183 +00:13:43,540 --> 00:13:49,200 +هو الـ number، يبقى كل type له dosage معينة، طب أنا + +184 +00:13:49,200 --> 00:13:54,190 +هأعطيكم مثال كمان، اختر شوية، إحنا ناقشنا مرة الباسيلس + +185 +00:13:54,190 --> 00:13:58,630 +أنتراكس، صح؟ اللي بسبب الأنثراكس، مين عندنا؟ الـ spore + +186 +00:13:58,630 --> 00:14:05,810 +former، هذه الـ spores تبعتها، إذا بتدخل عبر الجلد + +187 +00:14:05,810 --> 00:14:10,670 +بيكفي منها 100 ألف أو أقل من 100 ألف أنهم يعملوا + +188 +00:14:10,670 --> 00:14:16,330 +infection، إذا دخل عبر الجهاز التنفسي بيحتاج إلى + +189 +00:14:16,330 --> 00:14:22,430 +مليون spore، 100 ألف لمليون، رقم مختلف عن بعض كثير + +190 +00:14:23,210 --> 00:14:27,290 +عشرة ملايين عشان يعمل gastro-intestinal anthrax + +191 +00:14:27,290 --> 00:14:32,130 +يبقى الـ portal of entry، مكان دخول الميكروب كمان له + +192 +00:14:32,130 --> 00:14:36,830 +علاقة بمين؟ بالعدد، فهي نفس الميكروب نتحدثنا عن نفس + +193 +00:14:36,830 --> 00:14:41,790 +الميكروب، لما دخل من الجلد الرقم كان أقل، لما دخل + +194 +00:14:41,790 --> 00:14:46,850 +من الـ lungs زاد الرقم، لما دخل من الفم عبر الـ + +195 +00:14:46,850 --> 00:14:50,010 +intestine زاد الرقم أكثر وأكثر، إذا الـ portal of + +196 +00:14:50,010 --> 00:14:54,730 +entry كمان عملية مهمة، كل اللي ذكرته هنا عشان + +197 +00:14:54,730 --> 00:15:02,210 +أقول لكم أنه المصطلحات اللي + +198 +00:15:02,210 --> 00:15:07,590 +كلمة pathogen و non pathogen هي مصطلحات للأسف مش + +199 +00:15:07,590 --> 00:15:12,920 +absolute، بمعنى أنه أنا نفسي أكون absolute، أقول + +200 +00:15:12,920 --> 00:15:17,180 +أبيض يعني non-pathogen، أسود يعني pathogen، لكن + +201 +00:15:17,180 --> 00:15:23,160 +للأسف بيدخل إلينا فيروسات وميكروبات إلى أجسامنا، + +202 +00:15:23,160 --> 00:15:26,920 +تمام؟ وبأعداد كبيرة، مبيعات كبيرة، ومعروفة أنها + +203 +00:15:26,920 --> 00:15:29,380 +بتسبب أمراض، لكن ممكن ما تسبب أننا إيش إحنا؟ + +204 +00:15:29,380 --> 00:15:35,150 +أمراض، مريض الـ AIDS، الميكروبات اللي ما بتسببش أمراض + +205 +00:15:35,150 --> 00:15:38,110 +إلنا إحنا بتسبب له أمراض، اللي إحنا نقولها non + +206 +00:15:38,110 --> 00:15:45,010 +pathogen، عشان أوضح بشكل أنه نطلع من المحاضرة هذه + +207 +00:15:45,010 --> 00:15:48,330 +فاهمين أن الـ pathogen في منه absolute، دائما + +208 +00:15:48,330 --> 00:15:49,570 +pathogen، في منه + +209 +00:15:53,490 --> 00:15:57,330 +أقرب إلى الـ pathogen أو أقرب إلى الـ non pathogen + +210 +00:15:57,330 --> 00:16:02,130 +إذا وصلنا لهذا المفهوم بنرتاح كثير في المستقبل، الـ + +211 +00:16:02,130 --> 00:16:05,350 +pathogen هو اللي بيسبب infection، الـ non اللي + +212 +00:16:05,350 --> 00:16:08,250 +بيسببش، الـ opportunistic اللي بيسببش اللي لاجل عند + +213 +00:16:08,250 --> 00:16:12,090 +الناس اللي عندهم جهاز مناعي ضعيف، منها انتهازية + +214 +00:16:12,090 --> 00:16:16,330 +opportunistic pathogen، واللي بتسبب عند الـ + +215 +00:16:16,330 --> 00:16:19,510 +immunocompromised، immunocompromised عكسها + +216 +00:16:19,510 --> 00:16:25,230 +immunocompetent، إيه + +217 +00:16:25,230 --> 00:16:28,670 +يعني competent؟ فعليه، الجهاز المناعي عنده فعليه + +218 +00:16:28,670 --> 00:16:29,990 +immunocompromised + +219 +00:16:33,260 --> 00:16:37,500 +الـ pathogenicity هي قدرة الكائن المعدي الـ + +220 +00:16:37,500 --> 00:16:43,520 +infectious agent to cause disease، طبعا قدرته كمان + +221 +00:16:43,520 --> 00:16:47,260 +مرة ما إحنا قولناه بشوية أن فيه اختلافات، هي واحد بـ + +222 +00:16:47,260 --> 00:16:50,920 +.. بميكروب واحد، والثاني بـ .. بمليون microbe عشان + +223 +00:16:50,920 --> 00:16:55,740 +يعمل infection، ففيه علاقة بالـ virulence، الـ + +224 +00:16:55,740 --> 00:16:57,480 +virulence .. الـ virulence + +225 +00:17:00,140 --> 00:17:03,080 +للـ pathogenicity، سنين pathogen، لكن واحد virulence + +226 +00:17:03,080 --> 00:17:07,280 +تبعه أعلى من مين؟ من الثاني، ما الذي حدد أن الـ + +227 +00:17:07,280 --> 00:17:10,460 +virulence بتبع هذا الميكروب أعلى من الآخر؟ وجود + +228 +00:17:10,460 --> 00:17:16,060 +عدد من الـ virulence factors، فاكرين الـ virulence + +229 +00:17:16,060 --> 00:17:21,470 +factors؟ الـ flagella، فيرولينز فاكتور، الاندوتوكسين + +230 +00:17:21,470 --> 00:17:25,090 +فيرولينز فاكتور، الكبسول، فيرولينز فاكتور، الـ + +231 +00:17:25,090 --> 00:17:29,390 +بالايتات الاتتشمنت، فيرولينز فاكتور، بعض الانزيم، الـ + +232 +00:17:29,390 --> 00:17:36,090 +spores، كثير من التراكيب سميناها إيش؟ أي + +233 +00:17:36,090 --> 00:17:44,530 +شيء بيزيد من قدرة البكتيريا على الـ survival، فهو + +234 +00:17:44,530 --> 00:17:49,920 +فيرولينز فاكتور، الآن البكتيريا هجمت، البكتيريا أوذيت + +235 +00:17:49,920 --> 00:17:54,040 +تحولت من vegetative إلى spore، الـ spore is more + +236 +00:17:54,040 --> 00:17:58,520 +resistant، صح؟ وبالتالي هيكون قتلها صعب، روح الخطر + +237 +00:17:58,520 --> 00:18:01,560 +بتعود ترجع مرة أخرى، وبالتالي تعتبر virulence + +238 +00:18:01,560 --> 00:18:08,780 +factor، الفاكرين الكلمة جرعة؟ لص، قولنا أننا زمك؟ + +239 +00:18:08,780 --> 00:18:15,140 +هدف مين؟ في الـ disinfectants وفي الـ antiseptics + +240 +00:18:15,140 --> 00:18:18,380 +اللي استخدمناهم في المحاضرة السابقة، إحنا الآن الـ + +241 +00:18:18,380 --> 00:18:23,720 +dose هنا بدنا ننظر لها بنظرة شوية مختلفة، تسمعوا + +242 +00:18:23,720 --> 00:18:26,720 +بالـ lethal dose والـ infectious dose، وده أول مرة + +243 +00:18:26,720 --> 00:18:34,040 +مصطلحها لسه، طيب الـ LD50 والـ ID50، خليكم معايا دي + +244 +00:18:34,040 --> 00:18:40,670 +بتمركب أنا، ودي بت meet far، أعطيت الـ meat fire جرعة + +245 +00:18:40,670 --> 00:18:49,670 +X، ماتوا خمسين وظلوا خمسين، الجرعة X هذه بسميها LD50 + +246 +00:18:49,670 --> 00:18:56,290 +lethal dose اللي قتلت نصف عدد حيوانات التجارب، LD50 + +247 +00:18:56,290 --> 00:18:59,350 +طبعا + +248 +00:18:59,350 --> 00:19:01,850 +إحنا ده .. ده .. ده .. ده .. ده .. ده .. ده .. ده + +249 +00:19:01,850 --> 00:19:02,650 +.. ده .. ده .. ده .. ده .. ده .. ده .. ده .. ده .. + +250 +00:19:02,650 --> 00:19:05,980 +ده .. بيروح تابعون الصيدلة بـ .. بـ .. لازم يكونوا + +251 +00:19:05,980 --> 00:19:09,580 +عارفين عشان يحددوا ماذا سميت الـ drugs وبيوصفوا الـ + +252 +00:19:09,580 --> 00:19:14,480 +drugs بالـ LD50 تبعهم كل ما قالت.. كل ما قلت الـ + +253 +00:19:14,480 --> 00:19:19,240 +LD50 كرقم تمام كل ما كان العامل بيكون more toxic + +254 +00:19:19,240 --> 00:19:25,860 +more toxic يعني مثلا نقول إنه الـ LD50 point zero + +255 +00:19:25,860 --> 00:19:29,760 +zero zero مثلا five grams ولا point five gram مين + +256 +00:19:29,760 --> 00:19:35,000 +أكثَر سمية؟ الـ point zero zero zero صح؟ لما الـ ID + +257 +00:19:35,000 --> 00:19:41,300 +50 تكون رقم صغير الحياة بتكون بأكثر صميمة الـ ID 50 + +258 +00:19:41,300 --> 00:19:47,400 +بدل ما هو قتل إحداث إصابة infection إحداث إصابة + +259 +00:19:47,400 --> 00:19:54,020 +يعني هي الجرعة اللازمة لإحداث إصابة in fifty + +260 +00:19:54,020 --> 00:19:55,860 +percent of the test animal + +261 +00:20:02,160 --> 00:20:04,960 +الـ drugs بقى هتكلم عن concentration، في البكتيري + +262 +00:20:04,960 --> 00:20:08,700 +هتكلم عن إيش؟ عن number، في البكتيري هتكلم عن + +263 +00:20:08,700 --> 00:20:11,580 +number، ممكن تقولوا على الـ number concentration + +264 +00:20:11,580 --> 00:20:17,220 +برضه، وده لأ، خمس خلايا في كل milli، من هنا تبقى + +265 +00:20:17,220 --> 00:20:19,960 +تبقى ريانيه concentration، بس لكن في الآخر هنقول + +266 +00:20:19,960 --> 00:20:23,890 +عنه number ماذا هو الفرق بين الـ ID 50 و الـ ID 50؟ + +267 +00:20:23,890 --> 00:20:29,810 +الـ ID 50 هي نسبة القتل لـ 50% بينما الـ ID 50 هي + +268 +00:20:29,810 --> 00:20:33,430 +نسبة إحداث الـ infection بـ 50% بس أنا بدأ أرجع + +269 +00:20:33,430 --> 00:20:37,430 +برجوع وأعرض + +270 +00:20:37,430 --> 00:20:40,210 +اللوحة هذه اللي قدامنا هذه اللوحة اللي قدامنا بدنا + +271 +00:20:40,210 --> 00:20:46,320 +نطلع عليها من منظور كتير كويس أحنا عايشين الآن في + +272 +00:20:46,320 --> 00:20:50,820 +مجتمع وفي المجتمع فينا normal flora معينة، normal + +273 +00:20:50,820 --> 00:20:54,850 +flora معينة لو نبنا في المستشفى لأي غراب من الأغراض + +274 +00:20:54,850 --> 00:20:58,090 +وكلمة نبنا في المستشفى مش ضروري أنها مرض معدي + +275 +00:20:58,090 --> 00:21:02,070 +ممكن تكون إيدنا انكسرة صح ولا عراب لا حادث حارق + +276 +00:21:02,070 --> 00:21:06,950 +أوى فبقولنا أن مكوثنا في المستشفى قد يغير من الـ + +277 +00:21:06,950 --> 00:21:09,310 +resident flora لحظة اللورا الأحمر اللى مولودة في + +278 +00:21:09,310 --> 00:21:13,710 +الجهة التانية مايكروبات ماكانت موجودة في الجهة هذه + +279 +00:21:13,710 --> 00:21:19,250 +أو زيادت هذه الميكروبات أو بقيت كما هي لكن تغيرت + +280 +00:21:19,250 --> 00:21:24,520 +طبيعتها إيش بقيت كمية وتغيرت طبيعتها؟ أنا أبقى + +281 +00:21:24,520 --> 00:21:30,120 +أعطي مثال كتير سهل، بس بعد ما نخلص المثال، ننساه و + +282 +00:21:30,120 --> 00:21:36,080 +ننتقل للحقيقة الفقران اللي في المنازل اللي بتكون + +283 +00:21:36,080 --> 00:21:42,020 +عايشة في معلبة الأدمين صعب جدا صيدها، لكن لو كانت + +284 +00:21:42,020 --> 00:21:46,480 +فقرا برية وحطينا لها فخ بتلاقي اتجاه بسهولة، سبب؟ + +285 +00:21:47,240 --> 00:21:54,640 +إن الفقران في المنزل أحنا أعداء لها فهي تعلمت + +286 +00:21:54,640 --> 00:22:00,920 +تقنيات البقاء أمامنا، أمام الخطر الدهن من البني + +287 +00:22:00,920 --> 00:22:04,760 +أدمين، من البشر، بس في البر احنا مافيش احتجاج + +288 +00:22:04,760 --> 00:22:08,960 +بيننا وبينها، وبالتالي اتعلمت ممكن كيف تحمي نفسها + +289 +00:22:08,960 --> 00:22:12,860 +من الطيور الجارحة، من بعض الحيوانات الأخرى، لكن ما + +290 +00:22:12,860 --> 00:22:16,780 +تعلمت كيف تحمي نفسها من الإنسان، فبنلجي الفار + +291 +00:22:16,780 --> 00:22:20,520 +بالفعل، ليه صار لإيه ممكن الفار في المنزل؟ لأنه + +292 +00:22:20,520 --> 00:22:27,310 +اتعلم من ال.. من ال.. من الأدمين بينما هدك ضاله + +293 +00:22:27,310 --> 00:22:32,170 +سادش تمام لما اتعلم طب تعالوا نطبق الموضوع على + +294 +00:22:32,170 --> 00:22:36,590 +البكتيريا البكتيريا في المجتمع والبكتيريا في + +295 +00:22:36,590 --> 00:22:39,990 +المستشفى في المستشفى بنستخدم أطنان من المضادة + +296 +00:22:39,990 --> 00:22:45,230 +الحيوية بني في المجتمع لاستخدام أقل فالآن البكتيريا + +297 +00:22:45,230 --> 00:22:50,190 +في المستشفيات تعلمت كيف تقاوم المناضات الحيوية كما + +298 +00:22:50,190 --> 00:22:54,850 +..كما تعلم الفقر تماما في المنزل أساليب ومكر + +299 +00:22:54,850 --> 00:22:59,190 +البشر ضده فصارت more resistant في المستشفيات + +300 +00:22:59,190 --> 00:23:02,450 +البكتيريا than the community، إذا الآن من حجي أنا + +301 +00:23:02,450 --> 00:23:06,810 +إذا أنا اكتسبت عدوى من المستشفى أقول إنها والله + +302 +00:23:06,810 --> 00:23:11,110 +هذه خاصة من المستشفى لإنه البكتيري المثابرة + +303 +00:23:11,110 --> 00:23:14,530 +بقالها وإن كان اسمها E.coli هتختلف عن + +304 +00:23:14,530 --> 00:23:17,790 +E.coli في من؟ في المجتمع، لذا قسموا الـ + +305 +00:23:17,790 --> 00:23:20,590 +infection إلى نوعين، community acquired من + +306 +00:23:20,590 --> 00:23:23,610 +المجتمع، من الجامعة، من الشارع، من البيت، من أي + +307 +00:23:23,610 --> 00:23:28,160 +حاجة لكن مش المستشفى والنقطة التانية من المستشفى + +308 +00:23:28,160 --> 00:23:34,000 +واسمها hospital acquired أو nosocomial والاسم + +309 +00:23:34,000 --> 00:23:39,140 +الحديث هي ثلاث أسامي الآن health care associated + +310 +00:23:39,140 --> 00:23:45,080 +infections H A I health care associated infections + +311 +00:23:45,080 --> 00:23:48,400 +ليش سميناها health care وبطلنا عن hospital + +312 +00:23:48,400 --> 00:23:54,190 +infection الآن الـ hospital هو مكان بيكون فيه رعاية + +313 +00:23:54,190 --> 00:23:57,630 +طبية صح ولا لا؟ رعاية طبية، طب هل هو الوحيد اللي + +314 +00:23:57,630 --> 00:24:01,490 +بيقدم رعاية طبية ومن ضمنها مغادرة هاوية؟ أكيد لأ، + +315 +00:24:01,490 --> 00:24:05,590 +دور العجزة.. دور العجزة بتقدم رعاية طبية، + +316 +00:24:05,590 --> 00:24:09,550 +الحضانات الـ nurseries بتقدم رعاية طبية قد يكون + +317 +00:24:09,550 --> 00:24:14,770 +أماكن للمدمنين بتجدم رعاية طبية إذا أي مؤسسة اللي + +318 +00:24:14,770 --> 00:24:17,770 +لها علاقة بالـ health care إيه الجبن اللي ميزها عن + +319 +00:24:17,770 --> 00:24:21,270 +مين؟ عن المؤسسات اللي ماعناش علاقة بالـ health care + +320 +00:24:21,270 --> 00:24:25,030 +إذا الـ community acquired الـ hospital acquired + +321 +00:24:25,030 --> 00:24:30,490 +التصنيف سببه واضح أن أي microbe يكتسب المستشفى + +322 +00:24:30,490 --> 00:24:34,730 +غالبا يكون أسوأ من اللي هو الـ microbe أنا بدي + +323 +00:24:34,730 --> 00:24:39,410 +أعطاكم بس مثال بسيط المجاري الـ waste water اللي + +324 +00:24:39,410 --> 00:24:44,370 +خارج من المستشفى غير اللي خارج من المنازل وفي + +325 +00:24:44,370 --> 00:24:47,110 +دراسة أترينها يمكن جابلت سبع سنوات أو تمان سنوات + +326 +00:24:47,110 --> 00:24:51,530 +في الجامعة وجدنا فروقات كبيرة في الـ resistant rate + +327 +00:24:51,530 --> 00:24:55,010 +لنفس البكتيريا يعني مثلا أخدنا E.coli و E.coli + +328 +00:24:55,010 --> 00:24:57,930 +أسينيتوباكتر وأسينيتوباكتر سوداموناس وسوداموناس + +329 +00:24:57,930 --> 00:25:02,430 +وجدنا resistance عالي لذلك يجب على المستشفيات أن + +330 +00:25:02,430 --> 00:25:06,610 +تقوم بمعالجة المياه العادمة تبعتها داخليا في + +331 +00:25:06,610 --> 00:25:11,400 +المستشفيات قبل ضقة إلى المجتمع يبقى تصنيف هذا + +332 +00:25:11,400 --> 00:25:15,080 +question مش كويس الميزة مع بعض ونكمل إن شاء الله + +333 +00:25:15,080 --> 00:25:16,400 +المرة القادمة بإذن الله نفس الأمور diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipbsvDeQAIA_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipbsvDeQAIA_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..5fd11e81937a87126cb95dba2c2ce0e86cd5b509 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/ipbsvDeQAIA_postprocess.srt @@ -0,0 +1,1332 @@ +1 +00:00:20,670 --> 00:00:23,410 +بسم الله الرحمن الرحيم نكمل ان شاء الله من حياتهم + +2 +00:00:23,410 --> 00:00:29,310 +الدائمة bacterial infection and immunityاللوحة + +3 +00:00:29,310 --> 00:00:32,030 +اللي أمامكوا هي العنوان .. لوحة العنوان فيها + +4 +00:00:32,030 --> 00:00:35,910 +جالوتين مهمين جدا اللوحة الو .. او الصورة اللي .. + +5 +00:00:35,910 --> 00:00:39,350 +اللي بتغير ألوانها في المنطقة هذه حاجة بيسموها + +6 +00:00:39,350 --> 00:00:41,810 +chain of transmission ايش هي ال chain of + +7 +00:00:41,810 --> 00:00:45,450 +transmission؟ سلسلة الانتقال، لو بينتقل الميكروب + +8 +00:00:45,450 --> 00:00:51,390 +من إنسان إلى إنسان، من حيوان إلى إنسان، تمام؟ في + +9 +00:00:51,390 --> 00:00:55,930 +سلسلة من .. او خطوات معينة لازم تحدث، يجب أن تحدث + +10 +00:00:57,020 --> 00:01:00,680 +هذه الخطوات بنسميها chain of transmission فالصورة + +11 +00:01:00,680 --> 00:01:03,740 +اللي قدامكم ممكن تلاقوها بشكل سلسل في ال internet + +12 +00:01:03,740 --> 00:01:06,960 +أو في أماكن أخرى لكن في الآخر بتعبر عن نفس المعنى + +13 +00:01:06,960 --> 00:01:10,880 +بتعبر عن نفس المعنى ليش سميناها سلسلة لإن لو + +14 +00:01:10,880 --> 00:01:14,540 +بيصينا السلسلة في مكان احنا بنقطع ال chain of + +15 +00:01:14,540 --> 00:01:18,950 +transmissionوبالتالي احنا من وجبنا لما نعمل + +16 +00:01:18,950 --> 00:01:22,810 +control لل micro organism او لل infection انه نفهم + +17 +00:01:22,810 --> 00:01:27,650 +ال infection cycle او ال chain of transmission فهم + +18 +00:01:27,650 --> 00:01:33,150 +معمق بحيث انه نفكر كيف ممكن نقطع هذا ال cycle في + +19 +00:01:33,150 --> 00:01:35,830 +اي جانب انه الأسهل انه الأرخص انه الأضمن انه + +20 +00:01:35,830 --> 00:01:39,650 +الأسرع كل هذه النقاط ممكن نفهمها ان شاء الله من + +21 +00:01:39,650 --> 00:01:44,570 +هذه المحاضرة الصورة التانية معبرة عن عين و فيروسات + +22 +00:01:45,900 --> 00:01:49,500 +الفيروسات اللي من ضمنها ال influenza اللي من ضمنها + +23 +00:01:49,500 --> 00:01:52,580 +الرشح انتوا كلكوا متخيلين انها بتنتقل اقابل مين ال + +24 +00:01:52,580 --> 00:01:58,080 +respiratory tract فقط، لكن ممكن finger to eye، ايش + +25 +00:01:58,080 --> 00:02:03,570 +يعني finger to eye؟ يعني واحد .. راكز ..فأسطح واحد + +26 +00:02:03,570 --> 00:02:06,950 +جنبه كان عاطس وحاطط الفيروس على الطاولة واحنا + +27 +00:02:06,950 --> 00:02:10,330 +لمسناه و بعد شويه أجى عنده شعور بالحكا ودخل إصبعه + +28 +00:02:10,330 --> 00:02:15,970 +في عينه نقل الفيروس من ال surface إلى ال .. ال .. + +29 +00:02:15,970 --> 00:02:18,450 +ال .. ال nucleus membrane في العينه وبالتالي يمكن + +30 +00:02:18,450 --> 00:02:22,370 +يعمل infection إذا هي source مهم لمن؟ لل infection + +31 +00:02:24,680 --> 00:02:29,240 +في العلاقات ما بين اكتر من مخلوقين او مخلوقين او + +32 +00:02:29,240 --> 00:02:33,040 +اكتر قد تنقسم العلاقات الى ثلاث أشكال اما + +33 +00:02:33,040 --> 00:02:36,560 +commensalism او parasitism او mutualism كلكوا + +34 +00:02:36,560 --> 00:02:40,660 +درستهم في أحياء ألف و درستهم في ثانوية و قبل ذلك + +35 +00:02:40,660 --> 00:02:46,280 +لكن اللى انا بركز عليها في ال medical microbiology + +36 +00:02:46,280 --> 00:02:46,860 +علاقة + +37 +00:02:49,640 --> 00:02:53,500 +ال Parasitism ليش؟ لأنه فيه effect، harmful + +38 +00:02:53,500 --> 00:02:57,080 +effect، واحد بيستفيد اللي هو ال pathogen ال + +39 +00:02:57,080 --> 00:03:00,360 +parasite و واحد بينضر اللي هو ال host اللي هو + +40 +00:03:00,360 --> 00:03:02,960 +الإنسان في الحالة و ابتدا ال medical microbiome قد + +41 +00:03:02,960 --> 00:03:08,500 +أضطرق بشيء من الذكر ل ال commensals اللي هي + +42 +00:03:08,500 --> 00:03:14,470 +بتستفيد هي و الجسم بندرش تمام؟و قد نتطرق إلى ال + +43 +00:03:14,470 --> 00:03:17,650 +mutualism زي ال normal flora احنا بنوفرلها مكان و + +44 +00:03:17,650 --> 00:03:21,550 +هي بتعطيلنا vitamin K بنوفرلها مكان و هي بتساعدنا + +45 +00:03:21,550 --> 00:03:25,570 +في الحماية من infection من others لكن في الغالب + +46 +00:03:25,570 --> 00:03:28,930 +تركيزنا هيكون على أي واحدة منهم على اللي هي ال + +47 +00:03:28,930 --> 00:03:35,010 +palestine طيب مدام سبب نتكلمين على ال normal flora + +48 +00:03:35,010 --> 00:03:38,210 +كلمة flora بنات معناها باللغة العربية نباتات فلما + +49 +00:03:38,210 --> 00:03:41,510 +بيقول flora أو فلسطين و بمكاننا بيقول نباتات + +50 +00:03:41,510 --> 00:03:42,170 +فلسطين + +51 +00:03:47,960 --> 00:03:54,600 +الإنسان بشكل دائم هو الاسم القديم normal flora + +52 +00:03:54,600 --> 00:04:00,880 +طبعا لأنه زمان كانت تدرس البكتيريا تحت علم البطن + +53 +00:04:00,880 --> 00:04:07,200 +يعني علم النبات لأنهم كانوا صنفوها أيضا نباتفاطلق + +54 +00:04:07,200 --> 00:04:10,120 +اي لقب flora لكن الان و بعد ما احنا خلاص يعني صارت + +55 +00:04:10,120 --> 00:04:14,740 +الى مملكة لحاله تمام صار من الجدير انه او من + +56 +00:04:14,740 --> 00:04:17,360 +الأشدر ان احنا نسميها اسم خاص فيها فسموها + +57 +00:04:17,360 --> 00:04:21,900 +microbiota فسماء سواء اسمته normal flora او normal + +58 +00:04:21,900 --> 00:04:26,560 +microbiota المعنى هو واحد من فوائد جبل من الفوائد + +59 +00:04:26,560 --> 00:04:29,160 +انا قولت ان الجسم بنقسم الى قسمين مرة ده بتذكروا + +60 +00:04:29,160 --> 00:04:32,680 +بنقسم الى sterile و non sterile ال non sterile + +61 +00:04:32,680 --> 00:04:37,080 +بالتأكيد بحتوى على normal floraو ال normal flora + +62 +00:04:37,080 --> 00:04:41,040 +إلها فوائد عظيمة، يعني مش موجودة هيك بس عبثا، لأ + +63 +00:04:41,040 --> 00:04:45,860 +إلها فوائد عظيمة و كثيرة، من أهم فوائدها، بديش + +64 +00:04:45,860 --> 00:04:48,980 +أقول رقم واحد، رقم اتنين، رقم اتنين هي من أهم + +65 +00:04:48,980 --> 00:04:52,780 +فوائدها، حاجة بيسميها colonization resistance، + +66 +00:04:52,780 --> 00:04:56,740 +بالعربي مقاومة الاستعمار، يعني مش لحاجة أهل فلسطين + +67 +00:04:56,740 --> 00:04:59,700 +بتقاوم الاستعمار، كمان فينا اللي هو ال normal + +68 +00:04:59,700 --> 00:05:03,160 +microbiota، ما معناه مقاومة الاستعمار بهذا أوجه + +69 +00:05:03,160 --> 00:05:10,570 +المفهوم تبقىتخيلوا أنكم كلكم من الأصدقاء، تمام؟ و + +70 +00:05:10,570 --> 00:05:14,870 +أنكم أبين الكراسي اللي في القاعة دي كلها، لو إيجي + +71 +00:05:14,870 --> 00:05:17,630 +واحد من الأعداء بدي يحطله موطق قدم، بيلاجي مكان + +72 +00:05:17,630 --> 00:05:21,950 +يقعد فيه، يبقى وجودكم في هذا المكان فقط وجودكم، + +73 +00:05:21,950 --> 00:05:27,050 +تمام؟محتلين مواقع معينة في هذا المكان مانع آخرين + +74 +00:05:27,050 --> 00:05:32,330 +من انهم يستوطنوا و يستعمروا كولونايزايشن و أول + +75 +00:05:32,330 --> 00:05:36,330 +خطوة في ال infection كولونايزايشن و أول خطوة في + +76 +00:05:36,330 --> 00:05:40,630 +الكولونايزايشن attachment مانجلاش مكان نقعد فيه + +77 +00:05:40,630 --> 00:05:46,650 +تمام ال pathogen مع السلام مش بس هيك لو كان فينا + +78 +00:05:46,650 --> 00:05:51,650 +pathogen كمان و جايب معاه عداد كبيرة يعني ممكن + +79 +00:05:51,650 --> 00:05:57,320 +ينافسفاكرين لما درسنا منتجات البكتيريا اللي من + +80 +00:05:57,320 --> 00:06:00,520 +ضمنها ال toxins درسنا منتج اسمه bactericin رقم + +81 +00:06:00,520 --> 00:06:08,680 +أربع رقم تلاتة bactericin اللي هي antibiotic + +82 +00:06:08,680 --> 00:06:12,660 +-like سميناها antibiotic-like compounds produced + +83 +00:06:12,660 --> 00:06:12,940 +by + +84 +00:06:16,130 --> 00:06:21,010 +كده من المنافسة، كمان هذا بيساعد على أنه نمنع ال + +85 +00:06:21,010 --> 00:06:24,130 +colonization، إذا في عندي رقم اتنين و تلاتة + +86 +00:06:24,130 --> 00:06:29,890 +بيساهموا في منع الأمراض، في منع حدوث الأمراض، ليش؟ + +87 +00:06:29,890 --> 00:06:34,190 +بيمنعوا استيطان أو استعمار اللي هم ال pathogenic + +88 +00:06:34,190 --> 00:06:38,290 +microorganismرقم واحد بعض البكتيريا بتنتج vitamin + +89 +00:06:38,290 --> 00:06:42,970 +K و vitamin K جيد للانسان بيستفيد منه رقم اربع + +90 +00:06:42,970 --> 00:06:47,370 +بقول ان ال normal flora قد تساهم في ال stimulation + +91 +00:06:47,370 --> 00:06:52,390 +ل special organs او special tissue في مجموعة من ال + +92 +00:06:52,390 --> 00:06:57,670 +lymph nodes موجودة في الأمعاء اسمها biopatches و + +93 +00:06:57,670 --> 00:07:02,650 +هي specialized lymph node موجودين في الأمعاء ايش + +94 +00:07:02,650 --> 00:07:08,870 +ال lymph nodes؟بيوصفوها في علم المناعة انها the + +95 +00:07:08,870 --> 00:07:13,030 +budget check points نقاط التفتيش تبع الجسم ايش هي + +96 +00:07:13,030 --> 00:07:18,390 +نقاط التفتيش؟ تخيلوا ان القاع هذه مخصصة لطالبات + +97 +00:07:18,390 --> 00:07:21,610 +قسم العلم الطبي المخبرية سنة تانية ممنوع يدخلها + +98 +00:07:21,610 --> 00:07:24,990 +سنة تالتة ممنوع يدخلها سنة أولى فلازم يكون على + +99 +00:07:24,990 --> 00:07:30,730 +الباب حد يعمل check صح؟ على البطاقة انه فقط طالبات + +100 +00:07:30,730 --> 00:07:34,190 +سنة تانية مسمحوا لهم فال .. ال .. ال .. ال .. ال + +101 +00:07:34,190 --> 00:07:37,180 +..الـ Lymph node اللي مروضة في ال bio patches + +102 +00:07:37,180 --> 00:07:41,800 +بتكون بدورة عظيم جدا في مناء العدوى و خاصة انا + +103 +00:07:41,800 --> 00:07:44,920 +هنتكلم عن ال intestinal tract مليارات و ملايين من + +104 +00:07:44,920 --> 00:07:49,640 +الانواع من البكتيريا وبالتالي هي نوع الميكروبيوتر + +105 +00:07:49,640 --> 00:07:53,880 +عملت stimulation لهذا النسيج يعني خلته دايما اه + +106 +00:07:53,880 --> 00:07:56,800 +متحفز وساعد في ال development تبعه + +107 +00:08:04,260 --> 00:08:07,620 +colonization resistance ذكرتها في الأمعاب لكن + +108 +00:08:07,620 --> 00:08:11,700 +تذكروا الأنثى كمان إيها خاصية الفمية الفاجاينة + +109 +00:08:11,700 --> 00:08:14,820 +فيها normal microbiota و بتعمل colonization + +110 +00:08:14,820 --> 00:08:19,300 +resistance الستات الحوامل بيصير عندهم urinary + +111 +00:08:19,300 --> 00:08:22,000 +tract infection أو vagina infection أكتر من اللي + +112 +00:08:22,000 --> 00:08:25,560 +مش حوامل عارفين ليش؟ لأن ال hormonal changes اللي + +113 +00:08:25,560 --> 00:08:29,920 +بتصير عندها بتأثر على ال normal flora وبالتالي + +114 +00:08:29,920 --> 00:08:33,950 +بيصير ال normal flora مش طبيعيةبصير الابتزان تبعها + +115 +00:08:33,950 --> 00:08:38,210 +في خلال فبصير فينا إمكانية colonization ل other + +116 +00:08:38,210 --> 00:08:43,690 +microorganisms هحاكيلكوا كمان نقطة كتير مهمة + +117 +00:08:43,690 --> 00:08:51,970 +concept بدنا نفهمها كويس لو إحنا اتنين شخصين بدنا + +118 +00:08:51,970 --> 00:08:56,430 +نروح على الغرف التانية و بدنا نعمله مشكلة مشكلة + +119 +00:08:56,430 --> 00:08:59,760 +لكوا كديش البنات اللي قدامهم خمسين بناتراحوا + +120 +00:08:59,760 --> 00:09:02,660 +البنتين هؤلاء المسكين كل واحدة معاها جلم ولا سكينة + +121 +00:09:02,660 --> 00:09:06,680 +ولا حاجة ورفعت بإن أنا أقتلكوا 150 إبنة، ليش + +122 +00:09:06,680 --> 00:09:09,900 +بيكونوا عاملهم واقع لعمد؟ لأ لأ مش بصيروا يضحكوا، + +123 +00:09:09,900 --> 00:09:15,520 +جانوا على أنفسهم، هم أعلنوا عن عدوتهم تمام؟ وهم لا + +124 +00:09:15,520 --> 00:09:18,580 +يمتلكوا من القوة شيء، تعرفوا إن البكتيريا أذكى + +125 +00:09:18,580 --> 00:09:23,480 +منكم، من البنتين جزء اللي راحوا يعنيالبكتيريا لن + +126 +00:09:23,480 --> 00:09:26,640 +تقلل عن نفسها .. و أقولكوا ال analogy تبعها + +127 +00:09:26,640 --> 00:09:30,180 +للبكتيريا، لو خليتين بكتيريتين قرروا أنهم .. دخلوا + +128 +00:09:30,180 --> 00:09:32,820 +الدسيل، و قرروا أنهم ينطلوا التوكسين، يعني رفعوا + +129 +00:09:32,820 --> 00:09:38,720 +السلاح تبعهم، التوكسين لن يكون له أي تأثير، لأن + +130 +00:09:38,720 --> 00:09:43,240 +كميته إيش هتكون؟ من two cells، قليل جدا، لكن هيكون + +131 +00:09:43,240 --> 00:09:46,020 +له أثر أنهم رفعوا السكين، بمعنى آخر جاز المناعة + +132 +00:09:46,020 --> 00:09:52,440 +تنبه لهم، فقتلوايبجي أعلنهم عن نفسهم مبكرا كان سيئ + +133 +00:09:52,440 --> 00:09:57,060 +سبب في موتهم فالبكتيريا ذكية البكتيريا لن تعلن عن + +134 +00:09:57,060 --> 00:10:01,400 +نفسها إلا إذا وصل عددها يبجي معنا الحكي كلمة وصل + +135 +00:10:01,400 --> 00:10:06,860 +عددها يبجي معنا معاها تقنية عندها تقنية تعرف فيها + +136 +00:10:06,860 --> 00:10:09,560 +من خلالها الأدب تبعها انتوا ماعندكوا التقنية هذه + +137 +00:10:09,560 --> 00:10:12,940 +يعني لو غمنا واحدة منكم احنا وقلنا للطالبات ادخلوا + +138 +00:10:12,940 --> 00:10:16,220 +الصف يلا بنت انت اللي مغمى أكمل واحدة في الصف مش + +139 +00:10:16,220 --> 00:10:20,040 +هتعرفوا بس البكتيريا بتعرفواالظهرة بنسميها Quorum + +140 +00:10:20,040 --> 00:10:25,640 +Sensing Quorum Sensing إحساس بالعدد الكلي Quorum + +141 +00:10:25,640 --> 00:10:30,440 +Sensing طبعا حاجة وقت نشرحها بتفاصيل أكتر كيف بتتم + +142 +00:10:30,440 --> 00:10:33,860 +لكن بنلفها مدى النقطة إيش .. إيش الدلالة اللي أنا + +143 +00:10:33,860 --> 00:10:38,440 +بدي أوصلها في هذا الموضوع ال normal microbiota + +144 +00:10:38,440 --> 00:10:44,660 +موجودة بأنواع مختلفة في مكان واحد أنواع مختلفة + +145 +00:10:44,660 --> 00:10:50,440 +بعداد محددة بعداد محددةهذه الأعداد المحددة غير + +146 +00:10:50,440 --> 00:10:56,360 +كافية للإعلان عن نفسها لإنتاج virulence factors + +147 +00:10:56,360 --> 00:11:03,200 +عوامل الشراسة اللي من ضمنها ال toxins نفترض الشجهة + +148 +00:11:03,200 --> 00:11:09,680 +هذه هم المجموعة اللي عاجلة و ممتازة وجاينة دور + +149 +00:11:09,680 --> 00:11:15,770 +كويس لإن عددهم قليلجبنا دموع و رشينا عليكم انتوا + +150 +00:11:15,770 --> 00:11:18,350 +المجموعة الكبرى اللى كانت مسيطرة على المجموعه دموع + +151 +00:11:18,350 --> 00:11:23,820 +مانعاها انها تكبر و موتناالان الغذاء اللى كان مخصص + +152 +00:11:23,820 --> 00:11:27,820 +لكوا المساحة صارت فاضية مين هاستغلها؟ هاستغل عددهم + +153 +00:11:27,820 --> 00:11:31,920 +ضعف او اضعاف عندهم الأول اذا وصل العدد ل column + +154 +00:11:31,920 --> 00:11:35,060 +sensing بيبطلوا عاجلين وكويسين ايش بيصيروا الان + +155 +00:11:35,060 --> 00:11:40,100 +ينتجوا ال virus factors اللى من ضمنها ال toxin + +156 +00:11:40,100 --> 00:11:45,240 +لذلك هناك أمراض قد تنشأ من وجود خلل في ال normal + +157 +00:11:45,240 --> 00:11:49,860 +microbiota وصلت صورة بعدين هم normal لما بيكونوا + +158 +00:11:49,860 --> 00:11:53,700 +في مكانهم normalلما بطلع من مكانهم إلى مكان آخر + +159 +00:11:53,700 --> 00:11:58,860 +ببطله normal يعني أعطيكم مثال واضح وصري الشريشيا + +160 +00:11:58,860 --> 00:12:02,820 +كولاي is a normal flora of the intestinal tract + +161 +00:12:02,820 --> 00:12:06,640 +الشريشيا + +162 +00:12:06,640 --> 00:12:09,200 +كولاي is number one cause of urinary tract + +163 +00:12:09,200 --> 00:12:14,320 +infection يبقى لما انتقلت من ال intestine إلى ال + +164 +00:12:14,320 --> 00:12:18,360 +urinary tract عملت infectionيبقى مش كلهم normal + +165 +00:12:18,360 --> 00:12:20,900 +flora يعني normal flora يعني مصطلح تطلق عليهم + +166 +00:12:20,900 --> 00:12:24,220 +normal flora لأ في مكانه هو normal flora و ربما + +167 +00:12:24,220 --> 00:12:30,060 +يكون العدد كمان عامل محدد مهم و بما أن احنا ذكرنا + +168 +00:12:30,060 --> 00:12:34,260 +كلمة عدد تعالوا نتكلم على ايش الأوامن التلاتة أصلا + +169 +00:12:34,260 --> 00:12:40,340 +اللي بتساهم في ال infection ال host immunity ال + +170 +00:12:40,340 --> 00:12:48,750 +type ofmicroorganism وكلمة type يحق لشراسته و ال + +171 +00:12:48,750 --> 00:12:52,170 +number اللي دخل ال number تبع ال microorganism هاي + +172 +00:12:52,170 --> 00:12:57,970 +تلت عوامل كمان مرة ال host immunityالـType وكنا + +173 +00:12:57,970 --> 00:13:00,690 +كامرة الـType و الـOrganism كنا إشارة لمن؟ + +174 +00:13:00,690 --> 00:13:06,250 +للـVirals رقم تلاتة الـNumber في ضعفات أخرى لكن هي + +175 +00:13:06,250 --> 00:13:10,750 +أهم التلت عوامل اللي بتساهم أنه يصير Infection و + +176 +00:13:10,750 --> 00:13:12,690 +لا ما يصير إيش Infection و أعطيكم إتالة بسيطة عشان + +177 +00:13:12,690 --> 00:13:18,930 +نسينا أن الشجلوسز شجلة تمام حتى عند الإنسان الصحيح + +178 +00:13:18,930 --> 00:13:22,290 +الإنسان اللي عنده جهاز ملاعق كويس ال number + +179 +00:13:22,290 --> 00:13:27,840 +requiredالشجلة number شوية من one to ten cells + +180 +00:13:27,840 --> 00:13:34,000 +خلية لعشر خلايا في المقابل لو ال type اختلف من + +181 +00:13:34,000 --> 00:13:38,120 +shigella ل vibrio محتاج لمليون محتاج لمليون ايش + +182 +00:13:38,120 --> 00:13:43,540 +العامل اللي اختلف نوع البكتيريا فادة الاختلاف اللي + +183 +00:13:43,540 --> 00:13:49,200 +هو ال number يبقى كل type له dosage معينة طب انا + +184 +00:13:49,200 --> 00:13:54,190 +هتعطيكم مثال كمان اختر شويةاحنا نقشنا مرة البصيلاس + +185 +00:13:54,190 --> 00:13:58,630 +أنتراكسي صح اللي بسبب أنتراكس مينعناج ال sport + +186 +00:13:58,630 --> 00:14:05,810 +former هذه ال sports تبعتها إذا بتدخل عبر الجلد + +187 +00:14:05,810 --> 00:14:10,670 +بيكفي منها 100 ألف أو أقل من 100 ألف انهم يعملوا + +188 +00:14:10,670 --> 00:14:16,330 +infection إذا دخل عبر الجهاز التنفسي بيحتاج إلى + +189 +00:14:16,330 --> 00:14:22,430 +مليون sport 100 ألف لمليونرقم مختلف عن بعض كتير + +190 +00:14:23,210 --> 00:14:27,290 +عشرة مليون عشان يعمل gastro-intestinal anthrax + +191 +00:14:27,290 --> 00:14:32,130 +يبقى ال portal of entry مكان دخول الميكروب كمان له + +192 +00:14:32,130 --> 00:14:36,830 +علاقة بمين؟ بالعدد فهي نفس الميكروب تتحدثنا عن نفس + +193 +00:14:36,830 --> 00:14:41,790 +الميكروب لما دخل من الجلد الرقم كان أقلهم لما دخل + +194 +00:14:41,790 --> 00:14:46,850 +من ال lungs زاد الرقم لما دخل من الفم عبر ال + +195 +00:14:46,850 --> 00:14:50,010 +intestine زاد الرقم أكتر وأكتر إذا ال portal of + +196 +00:14:50,010 --> 00:14:54,730 +entry كمان عملية مهمةكل اللي ذكرته هنا عشان + +197 +00:14:54,730 --> 00:15:02,210 +أقولكوا أنه المصطلحات اللي + +198 +00:15:02,210 --> 00:15:07,590 +كلمة pathogen و non pathogen هي مصطلحات للأسف مش + +199 +00:15:07,590 --> 00:15:12,920 +absoluteبمعنى أنه أنا نفسي أكون absolute، أقول + +200 +00:15:12,920 --> 00:15:17,180 +أبيض يعني non-pathogen، أسود يعني pathogen، لكن + +201 +00:15:17,180 --> 00:15:23,160 +للأسف بيدخل إلينا فيروسات وmicrobات إلى أجسامنا، + +202 +00:15:23,160 --> 00:15:26,920 +تمام؟ و بأعياد كبيرة، مبيعات كبيرة، و معروفة أنها + +203 +00:15:26,920 --> 00:15:29,380 +بتسبب أمراض، لكن ممكن ما تسبب أننا إيش احنا؟ + +204 +00:15:29,380 --> 00:15:35,150 +أمراض، مريض ال aidsالميكروبات اللي ما بتسببش أمراض + +205 +00:15:35,150 --> 00:15:38,110 +إلنا إحنا بتسببله أمراض اللي بجينا نقولها non + +206 +00:15:38,110 --> 00:15:45,010 +pathogen عشان أوضح بشكل أنه نطلع من المحاضرة هذه + +207 +00:15:45,010 --> 00:15:48,330 +فاهمين أن ال pathogen في منه absolute دايما + +208 +00:15:48,330 --> 00:15:49,570 +pathogen في منه + +209 +00:15:53,490 --> 00:15:57,330 +أقرب إلى ال pathogen أو أقرب إلى ال non pathogen + +210 +00:15:57,330 --> 00:16:02,130 +إذا وصلنا لهذا المفهوم بنرتاح كتير في المستقبل ال + +211 +00:16:02,130 --> 00:16:05,350 +pathogen هو اللي بيسبب infection ال non اللي + +212 +00:16:05,350 --> 00:16:08,250 +بيسببش ال opportunistic اللي بيسببش اللي لاجل عند + +213 +00:16:08,250 --> 00:16:12,090 +الناس اللي عندهم جاذب منها إيه ضعيفة منها انتهازية + +214 +00:16:12,090 --> 00:16:16,330 +opportunistic pathogenواللي بتسبب عند ال + +215 +00:16:16,330 --> 00:16:19,510 +immunocompromised immunocompromised عكسها + +216 +00:16:19,510 --> 00:16:25,230 +immunocompetent إيه + +217 +00:16:25,230 --> 00:16:28,670 +يعني competent؟ فعليه الجيهة اللي بناها عنده فعليه + +218 +00:16:28,670 --> 00:16:29,990 +immunocompromised + +219 +00:16:33,260 --> 00:16:37,500 +الـ Pathogenicity هي قدرة الكائن المعدى الـ + +220 +00:16:37,500 --> 00:16:43,520 +infectious agent to cause disease طبعا قدرته كمان + +221 +00:16:43,520 --> 00:16:47,260 +مرة ما احنا قولناه بشوية ان فيه اختلافات هي واحد ب + +222 +00:16:47,260 --> 00:16:50,920 +.. بmicrobe واحد والتاني ب .. بمليون microbe عشان + +223 +00:16:50,920 --> 00:16:55,740 +يعمل infection ففيه علاقة بالـ virulence ال + +224 +00:16:55,740 --> 00:16:57,480 +virulence .. ال virulence + +225 +00:17:00,140 --> 00:17:03,080 +للpathogenicity، سنين pathogen، لكن واحد viralness + +226 +00:17:03,080 --> 00:17:07,280 +تبعه أعلى من مين؟ من التاني، ما الذي حدد أن ال + +227 +00:17:07,280 --> 00:17:10,460 +viralness بتبع هذا الميكروب أعلى من الآخر؟ وجود + +228 +00:17:10,460 --> 00:17:16,060 +عدد من ال viralness factors، فاكرين ال viralness + +229 +00:17:16,060 --> 00:17:21,470 +factors؟ ال flagellaفيرولينز فاكتور الاندوتكسين + +230 +00:17:21,470 --> 00:17:25,090 +فيرولينز فاكتور الكبسول فيرولينز فاكتور ال + +231 +00:17:25,090 --> 00:17:29,390 +بالايتات الاتتشمنت فيرولينز فاكتور بعض الانزيم ال + +232 +00:17:29,390 --> 00:17:36,090 +spores كتير من التراكيب سميناها ايش؟ اي + +233 +00:17:36,090 --> 00:17:44,530 +شيء بيزيد من قدرة البكتيريا على ال survival فهو + +234 +00:17:44,530 --> 00:17:49,920 +فيرولينز فاكتور الآن البكتيريا هجمتالبكتيريا أوذيت + +235 +00:17:49,920 --> 00:17:54,040 +فتحولت من vegetative إلى sport، ال sport is more + +236 +00:17:54,040 --> 00:17:58,520 +resistant، صح؟ وبالتالي هيكون قتلها صعب، روح الخطر + +237 +00:17:58,520 --> 00:18:01,560 +بتعود ترجع مرة أخر، وبالتالي تعتبر virulence + +238 +00:18:01,560 --> 00:18:08,780 +factor الفاكرين الكلمة جرعة؟ لص، قولنا أننا زمك؟ + +239 +00:18:08,780 --> 00:18:15,140 +هدف مين؟في ال disinfectants و في ال antiseptics + +240 +00:18:15,140 --> 00:18:18,380 +اللي استخدمناهم في المحاضرة السابقة احنا الآن ال + +241 +00:18:18,380 --> 00:18:23,720 +dose ان هنا بدنا ننظرلها بنظرة شوية مختلفة تسمعوا + +242 +00:18:23,720 --> 00:18:26,720 +بال lethal dose و ال infectious dose و ده أول مرة + +243 +00:18:26,720 --> 00:18:34,040 +مصنعتها لسه طيب ال LD50 و ال ID50 خليكم معايا دي + +244 +00:18:34,040 --> 00:18:40,670 +بتمركب أنا و دي بت meet farأعطيت ال meat fire جرعة + +245 +00:18:40,670 --> 00:18:49,670 +X ماتوا خمسين وظلوا خمسين الجرعة X هذه بسميها LD50 + +246 +00:18:49,670 --> 00:18:56,290 +lethal dose اللي قتلت نصف عدد حيانة تجارب LD50 + +247 +00:18:56,290 --> 00:18:59,350 +طبعا + +248 +00:18:59,350 --> 00:19:01,850 +احنا ده .. ده .. ده .. ده .. ده .. ده .. ده .. ده + +249 +00:19:01,850 --> 00:19:02,650 +.. ده .. ده .. ده .. ده .. ده .. ده .. ده .. ده .. + +250 +00:19:02,650 --> 00:19:05,980 +ده ..بيروح تابعون الصيدرة ب .. ب .. لازم يكونوا + +251 +00:19:05,980 --> 00:19:09,580 +عارفين عشان يحددوا ماذا سميت ال drugs وبيوصفوا ال + +252 +00:19:09,580 --> 00:19:14,480 +drugs بال LD50 تبعهم كل ما قالت .. كل ما قلت ال + +253 +00:19:14,480 --> 00:19:19,240 +LD50 كرقم تمام كل ما كان العامل بيكون more toxic + +254 +00:19:19,240 --> 00:19:25,860 +more toxic يعني مثلا نقول انه ال LD50 point zero + +255 +00:19:25,860 --> 00:19:29,760 +zero zero مثلا five grams ولا point five gram مين + +256 +00:19:29,760 --> 00:19:35,000 +أكتر سمية؟الـ point zero zero zero صح؟ لما ال ID + +257 +00:19:35,000 --> 00:19:41,300 +50 تكون رقم صغير الحياة بتكون بأكثر صميمة ال ID 50 + +258 +00:19:41,300 --> 00:19:47,400 +بدل ما هو قتل إحداث إصابة infection إحداث إصابة + +259 +00:19:47,400 --> 00:19:54,020 +يعني هي الجرعة اللازمة لإحداث إصابة in fifty + +260 +00:19:54,020 --> 00:19:55,860 +percent of the test animal + +261 +00:20:02,160 --> 00:20:04,960 +الدراجز بقى هتكلم عن concentration، في البكتيري + +262 +00:20:04,960 --> 00:20:08,700 +هتكلم عن إيش؟ عن number، في البكتيري هتكلم عن + +263 +00:20:08,700 --> 00:20:11,580 +number، ممكن تقولوا على ال number concentration + +264 +00:20:11,580 --> 00:20:17,220 +برضه، و ده لأ، خمس خلايا في كل milli، من هنا تبقى + +265 +00:20:17,220 --> 00:20:19,960 +تبقى ريانيه concentration، بس لكن في الآخر هنقول + +266 +00:20:19,960 --> 00:20:23,890 +عنه numberماذا هو الفرق بين ال ID 50 و ال ID 50؟ + +267 +00:20:23,890 --> 00:20:29,810 +ال ID 50 هي نسبة القتل ل 50% بينما ال ID 50 هي + +268 +00:20:29,810 --> 00:20:33,430 +نسبة إحداث ال infection ب 50% بس أنا بدأ أرجع + +269 +00:20:33,430 --> 00:20:37,430 +برجوع وأعرض + +270 +00:20:37,430 --> 00:20:40,210 +اللوحة هذه اللي قدامنا هذه اللوحة اللي قدامنا بدنا + +271 +00:20:40,210 --> 00:20:46,320 +نطلع عليها من منظور كتير كويساحنا عايشين الآن في + +272 +00:20:46,320 --> 00:20:50,820 +مجتمع وفي المجتمع فينا normal flora معينة، normal + +273 +00:20:50,820 --> 00:20:54,850 +flora معينةلو نبنا في المستشفى لأي غراب من الأغراض + +274 +00:20:54,850 --> 00:20:58,090 +و كلمة نبنا في المستشفى مش ضرورى أنها مرض معدي + +275 +00:20:58,090 --> 00:21:02,070 +ممكن تكون إيدنا انكسرة صح ولا عراب لا حادث حارق + +276 +00:21:02,070 --> 00:21:06,950 +أوى فبقولنا أن مكوثنا في المستشفى قد يغير من ال + +277 +00:21:06,950 --> 00:21:09,310 +resident flora لحظة اللورة الأحمر اللى مولودة في + +278 +00:21:09,310 --> 00:21:13,710 +الجهة التانية مايكروبات ماكانت موجودة في الجهة هذه + +279 +00:21:13,710 --> 00:21:19,250 +أو زيادت هذه الميكروبات أو بقيت كما هي لكن تغيرت + +280 +00:21:19,250 --> 00:21:24,520 +طبيعتهاإيش بقيت كمية و تغيرت طبيعتها؟ أنا أبقى + +281 +00:21:24,520 --> 00:21:30,120 +أعطي مثال كتير سهل، بس بعد ما نخلص المثال، ننساه و + +282 +00:21:30,120 --> 00:21:36,080 +ننتقل للحقيقة الفقران اللي في المنازل اللي بتكون + +283 +00:21:36,080 --> 00:21:42,020 +عايشة في معلبة الأدمين صعب جدا صيدها، لكن لو كانت + +284 +00:21:42,020 --> 00:21:46,480 +فقرا برية و حطينا لها فخ بتلاقي اتجاه بسهولة، سبب؟ + +285 +00:21:47,240 --> 00:21:54,640 +إن الفقران في المنزل أحنا أعداء لها فهي تعلمت + +286 +00:21:54,640 --> 00:22:00,920 +تقنيات البقاءأمامنا، أمام الخطر الدهن من البني + +287 +00:22:00,920 --> 00:22:04,760 +أدمين، من البشر، بس في البر احنا مافيش احتجاج + +288 +00:22:04,760 --> 00:22:08,960 +بيننا وبينها، وبالتالي اتعلمت ممكن كيف تحمي نفسها + +289 +00:22:08,960 --> 00:22:12,860 +من الطيور الجارحة، من بعض الحيوانات الأخرى، لكن ما + +290 +00:22:12,860 --> 00:22:16,780 +تعلمت كيف تحمي نفسها من الإنسان، فبنلجي الفار + +291 +00:22:16,780 --> 00:22:20,520 +بالفعل، ليه صار لإيه ممكن الفار في المنزل؟ لأنه + +292 +00:22:20,520 --> 00:22:27,310 +اتعلم من ال ..من ال .. من الأدمين بينما هدك ضاله + +293 +00:22:27,310 --> 00:22:32,170 +سادش تمام لما اتعلم طب تعالوا نطبق الموضوع على + +294 +00:22:32,170 --> 00:22:36,590 +البكتيريا البكتيريا في المجتمع و البكتيريا في + +295 +00:22:36,590 --> 00:22:39,990 +المستشفى في المستشفى بنستخدم أطنان من المضادة + +296 +00:22:39,990 --> 00:22:45,230 +الحيويةبنى فى المجتمع لاستخدام اقل فالان البكتيريا + +297 +00:22:45,230 --> 00:22:50,190 +فى المستشفيات تعلمت كيف تقاوم المناضات الحيوية كما + +298 +00:22:50,190 --> 00:22:54,850 +.. كما تعلم الفقر تماما فى المنزل أساليب ومكر + +299 +00:22:54,850 --> 00:22:59,190 +البشر ضدهفصارت more resistant في المستشفيات + +300 +00:22:59,190 --> 00:23:02,450 +البكتيريا than the community، إذا الآن من حجي انا + +301 +00:23:02,450 --> 00:23:06,810 +إذا انا اكتسبت عدوى من المستشفى أقول إنها والله + +302 +00:23:06,810 --> 00:23:11,110 +هادى خاصة من المستشفى لإنه البكتيرى المثابرة + +303 +00:23:11,110 --> 00:23:14,530 +بقالها وإن كان اسمها Aesthesia coli هتختلف عن + +304 +00:23:14,530 --> 00:23:17,790 +Aesthesia coli في من؟ في المجتمع، لذا قسموا ال + +305 +00:23:17,790 --> 00:23:20,590 +infection إلى نوعين، community acquired من + +306 +00:23:20,590 --> 00:23:23,610 +المجتمع، من الجامعة، من الشارع، من البيت، من أي + +307 +00:23:23,610 --> 00:23:28,160 +حاجةلكن مش المستشفى والنقطة التانية من المستشفى + +308 +00:23:28,160 --> 00:23:34,000 +واسمها hospital acquired أو nosocomial والاسم + +309 +00:23:34,000 --> 00:23:39,140 +الحديث هي ثلاث أسامي الان health care associated + +310 +00:23:39,140 --> 00:23:45,080 +infections H A I health care associated infections + +311 +00:23:45,080 --> 00:23:48,400 +ليش سميناها health care و بطلنا عن hospital + +312 +00:23:48,400 --> 00:23:54,190 +infectionالان ال hospital هو مكان بيكون فيه رعاية + +313 +00:23:54,190 --> 00:23:57,630 +طبية صح ولا لا؟ رعاية طبية، طب هل هو الوحيد اللي + +314 +00:23:57,630 --> 00:24:01,490 +بيقدم رعاية طبية ومن ضمنها مغادرة هاوية؟ أكيد لأ، + +315 +00:24:01,490 --> 00:24:05,590 +دور العجزة .. دور العجزة بتقدم رعاية طبية، + +316 +00:24:05,590 --> 00:24:09,550 +الحضانات ال nurseries بتقدم رعاية طبيةقد يكون + +317 +00:24:09,550 --> 00:24:14,770 +اماكن للمدمنين بتجدم رعاية طبية اذا اي مؤسسة اللي + +318 +00:24:14,770 --> 00:24:17,770 +لها علاقة بال health care ايه الجبن اللي ميزها عن + +319 +00:24:17,770 --> 00:24:21,270 +مين؟ عن المؤسسات اللي ماعناش علاقة بال health care + +320 +00:24:21,270 --> 00:24:25,030 +اذا ال community acquired ال hospital acquired + +321 +00:24:25,030 --> 00:24:30,490 +التصنيف سببه واضح ان اي microbe يكتسب المستشفى + +322 +00:24:30,490 --> 00:24:34,730 +غالبا يكون اسوأ من اللي هو ال microbe انا بدي + +323 +00:24:34,730 --> 00:24:39,410 +اعطاكم بس مثال بسيطالمجاري الـ waste water اللي + +324 +00:24:39,410 --> 00:24:44,370 +خارج من المستشفى غير اللي خارج من المنازلو في + +325 +00:24:44,370 --> 00:24:47,110 +دراسة اتراينها يمكن جابلت سبع سنوات او تمان سنوات + +326 +00:24:47,110 --> 00:24:51,530 +في الجامعة وجدنا فروقات كبيرة في ال resistant rate + +327 +00:24:51,530 --> 00:24:55,010 +لنفس البكتيريا يعني مثلا أخدنا E.coli و E.coli + +328 +00:24:55,010 --> 00:24:57,930 +أسينيتوباكتر و أسينيتوباكتر سوداموناس و سوداموناس + +329 +00:24:57,930 --> 00:25:02,430 +وجدنا resistance عالي لذلك يجب على المستشفيات أن + +330 +00:25:02,430 --> 00:25:06,610 +تقوم بمعالغة المياه العادمة تبعتها داخليا في + +331 +00:25:06,610 --> 00:25:11,400 +المستشفيات قبل ضقة إلى المجتمعيبقى تصنيف هذا + +332 +00:25:11,400 --> 00:25:15,080 +question مش كويس الميزة مع بعض ونكمل ان شاء الله + +333 +00:25:15,080 --> 00:25:16,400 +المرة القادمة بإذن الله نفس الامور + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY.srt new file mode 100644 index 0000000000000000000000000000000000000000..23d39229ad14d65cd1321f760b5963aa0c0738a7 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY.srt @@ -0,0 +1,1639 @@ +1 +00:00:20,590 --> 00:00:24,930 +بسم الله الرحمن الرحيم استكمل إن شاء الله موضوع + +2 +00:00:24,930 --> 00:00:28,990 +الـ Gram-Negative Bacilli الـ Enterobacteriaceae + +3 +00:00:28,990 --> 00:00:35,070 +بدأنا بالمشهورة الاشريشيا كولاي تحدثنا عن + +4 +00:00:35,070 --> 00:00:37,770 +الاشريشيا كولاي على أنها normal microbiota of the + +5 +00:00:37,770 --> 00:00:41,070 +intestinal tract وتحدثنا عنها إنها outside the + +6 +00:00:41,070 --> 00:00:44,110 +intestinal tract is opportunistic وهي المثال عليها + +7 +00:00:44,110 --> 00:00:46,770 +اللي قُلتها على اللوحة إنها تعمل urinary tract + +8 +00:00:46,770 --> 00:00:49,170 +infection بالعكس هي number one cause of urinary + +9 +00:00:49,170 --> 00:00:49,990 +tract infection + +10 +00:01:01,000 --> 00:01:08,040 +الآن أنا لما أخذت عينة اليوران في المعمل وقررت أنه + +11 +00:01:08,040 --> 00:01:12,400 +positive يعني في growth لبكتيريا وكانت + +12 +00:01:12,400 --> 00:01:15,420 +ايشريشيا كولاي كان ي differentiate between + +13 +00:01:15,420 --> 00:01:19,800 +cystitis and pyelonephritis بتقدروا تميزوا بينهم + +14 +00:01:19,800 --> 00:01:23,820 +أنت؟ صعب جداً لنميز بينهم من عينة UL المصلطة على + +15 +00:01:23,820 --> 00:01:27,120 +المختبر، واحنا ما عندناش معلومات إلا إن هو عينة UL، + +16 +00:01:27,120 --> 00:01:29,940 +لأنه إذا كان بيوليوفريتس أو سيستريتس، هتطلع + +17 +00:01:29,940 --> 00:01:30,900 +العينة + +18 +00:01:33,200 --> 00:01:38,260 +لا، بدي أوضح نقطة القاعدة هنا، أنه في limitations، + +19 +00:01:38,260 --> 00:01:43,120 +إيه الـ limitations؟ يعني حدود، محددات لحدود قدرتنا + +20 +00:01:43,120 --> 00:01:46,920 +في المزرعة، البعض مننا بيظن أنه إذا عزل مايكروب، + +21 +00:01:46,920 --> 00:01:50,600 +فهو هذا هو المسبب للـ infection، إذا عزل مايكروب، + +22 +00:01:50,600 --> 00:01:53,920 +فهو هذا هو الـ disease، لا الحقيقة أن هناك عامل آخر + +23 +00:01:53,920 --> 00:01:58,280 +لازم ننظر إليه بالاعتبار، مثل التفريق ما بين + +24 +00:01:58,280 --> 00:02:02,500 +cystitis و pyelonephritis نضطر نعمل فحص اسمه CRP + +25 +00:02:02,500 --> 00:02:05,820 +اللي هو C-reactive protein هتكلم عنه في مرحلة أخرى في + +26 +00:02:05,820 --> 00:02:06,940 +الـ micro pyruh + +27 +00:02:18,470 --> 00:02:21,890 +كانت المثال الثاني على أن الاشريشيا كولاي outside + +28 +00:02:21,890 --> 00:02:24,550 +the intestinal tract هي pathogen السببسيز والـ + +29 +00:02:24,550 --> 00:02:27,770 +meningitis وخاصة في الـ neonatal وبرضه ذكرنا + +30 +00:02:27,770 --> 00:02:30,970 +معلومة أن هي number one cause of neonatal + +31 +00:02:30,970 --> 00:02:35,250 +meningitis بس أكتفينا على حقيقة أن الـ strains اللي + +32 +00:02:35,250 --> 00:02:40,130 +بتسبب neonatal meningitis غالباً بتكون capsulated وعندها + +33 +00:02:40,130 --> 00:02:43,050 +K1 antigen واللي بيقول عنه هو الـ capsular + +34 +00:02:43,050 --> 00:02:48,460 +antigen الآن انتقلنا لمرحلة أنهم outside the + +35 +00:02:48,460 --> 00:02:50,500 +intestinal tract وانتقلنا لقاله mainly لـ inside + +36 +00:02:50,500 --> 00:02:53,480 +the intestinal tract احنا اتفقنا أنه في أربع من الـ + +37 +00:02:53,480 --> 00:02:57,300 +bacterial C احكي التلاتة الأولين فاكر إني أقول + +38 +00:02:57,300 --> 00:03:02,640 +اشريشيا كولاي وقلنا سالمونيلا شيجيلا يرسينيا هدول + +39 +00:03:02,640 --> 00:03:04,960 +التلاتة they are not normal in the intestinal + +40 +00:03:04,960 --> 00:03:07,700 +tract they cause intestinal tract infection ضفنا + +41 +00:03:07,700 --> 00:03:10,880 +إليهم في المرة الثانية الاشريشيا كولاي وقلنا some + +42 +00:03:10,880 --> 00:03:13,180 +strains مش كلهم some strains + +43 +00:03:17,960 --> 00:03:21,780 +طب نحط شق أمام الـ E. coli مثلاً قدامي على + +44 +00:03:21,780 --> 00:03:27,200 +لوحة بالبول انتروباثوجينيك E. coli طبعاً + +45 +00:03:27,200 --> 00:03:32,580 +هذا الاسم خادع الصحيح وبدنا نفكس عليه شوية من أي + +46 +00:03:32,580 --> 00:03:36,680 +ناحية خادع أنه بيعطيني حق أن أنا بتكلم عن كل الـ E. + +47 +00:03:36,680 --> 00:03:41,150 +coli اللي بتعمل infection في الـ intestine + +48 +00:03:41,150 --> 00:03:44,830 +لإن الـ pathogenic كان ممرضاً لكن وقع الأمر هو + +49 +00:03:44,830 --> 00:03:49,390 +المصطلح اخترع للتعبير عن ما أنا قلته الآن لكن + +50 +00:03:49,390 --> 00:03:54,370 +لجينا أصناف مختلفة فخلينا واحد من الأصناف بالاسم + +51 +00:03:54,370 --> 00:03:57,530 +نفسه enteropathogenic واخترعنا أسماء جديدة من + +52 +00:03:57,530 --> 00:04:01,490 +الأسماء لأنواع أخرى هنشوفهم كمان شوية فـ + +53 +00:04:01,490 --> 00:04:08,070 +enteropathogenic-EPEC هي أحد الأصناف وليس كل + +54 +00:04:08,070 --> 00:04:13,490 +الأصناف تعمل fever, infandiaria, vomiting, nausea, + +55 +00:04:14,030 --> 00:04:17,610 +non bloody diarrhea, non bloody stool يعني الـ + +56 +00:04:17,610 --> 00:04:23,430 +stool مش مدمم، ما فيش فيه، مش مصحوب بيه دم ناتج + +57 +00:04:23,430 --> 00:04:28,230 +غالباً عن destruction of surface microvilli ركزوا + +58 +00:04:28,230 --> 00:04:32,370 +معاه وتذكروا الـ intestinal tract الـ intestinal + +59 +00:04:32,370 --> 00:04:36,170 +tract فيها الـ villi والـ villi هي المسؤولة عن الـ + +60 +00:04:36,170 --> 00:04:38,790 +absorption للـ digested food + +61 +00:04:45,880 --> 00:04:49,000 +طب أنا ما أتوقعش أن يكون destruction كامل لكل الـ + +62 +00:04:49,000 --> 00:04:52,060 +microvilli فبيقل الـ absorption لما يقل الـ + +63 +00:04:52,060 --> 00:04:58,220 +absorption إيش بيزيد في الـ intestine؟ الأملاح، + +64 +00:04:58,220 --> 00:05:01,860 +فاكرين؟ فاكرين دي يعني إذا زاد الأملاح، لازم + +65 +00:05:01,860 --> 00:05:09,070 +الجسم يخففها، ده نازع المعتبر عليها في إدارة لكن + +66 +00:05:09,070 --> 00:05:12,450 +الأسباب بتكون متنوعة لماذا لما قل الامتصاص أو + +67 +00:05:12,450 --> 00:05:16,570 +لماذا تم التراكم بتكون أسباب مختلفة فهذه سببها أن + +68 +00:05:16,570 --> 00:05:20,910 +الـ microvilli اللي موجودة ما قدرت كيف ما قدرت؟ عن طريق + +69 +00:05:20,910 --> 00:05:25,390 +attachment بنوع خاص من الـ pili بيسمونه bundle + +70 +00:05:25,390 --> 00:05:32,280 +forming bundle معناها حزمة bundle-forming pili أو + +71 +00:05:32,280 --> 00:05:37,400 +البكتيريا بتعمل stimulation للـ Intracellular + +72 +00:05:37,400 --> 00:05:42,460 +Calcium Level و rearrangement للـ Intracellular + +73 +00:05:42,460 --> 00:05:42,900 +Actin + +74 +00:05:45,540 --> 00:05:48,060 +اللي أنا المرة الماضية قلت لكم عنها الـ type 3 + +75 +00:05:48,060 --> 00:05:51,080 +secretory system قلت لكم اتفرجوا عليها على اليوتيوب + +76 +00:05:51,080 --> 00:05:55,480 +أن البكتيريا بتعمل injection لبعض الـ proteins الـ + +77 +00:05:55,480 --> 00:06:00,300 +proteins هتعمل modulation أو modification للأكتين + +78 +00:06:00,300 --> 00:06:04,880 +بحيث أن تصبح أنها إيه؟ intracellular pathogenesis + +79 +00:06:04,880 --> 00:06:08,620 +يعني تاخد البكتيريا في معظم الـ + +80 +00:06:16,260 --> 00:06:21,700 +آخر من البكتيريا اسمه ETEC + +81 +00:06:21,700 --> 00:06:28,080 +اختلف عنها الأولانية بحرف الـ P معنى T تتذكر + +82 +00:06:28,080 --> 00:06:30,920 +الكوليرا؟ إذا كنت تتذكر الكوليرا، مدام الـ term + +83 +00:06:30,920 --> 00:06:35,060 +اليوم، what is the mechanism of action للكوليرا؟ + +84 +00:06:35,060 --> 00:06:36,200 +كيف الامتحان؟ + +85 +00:06:40,050 --> 00:06:43,710 +اللي بتعمل الـ .. برضه بتزيد في رجلات الأملاح برضه + +86 +00:06:43,710 --> 00:06:47,870 +.. كيف؟ وأنا .. أنا السؤال كيف؟ الـ mechanism يتطلب الـ + +87 +00:06:47,870 --> 00:06:52,990 +A1، يتطلب عن الأديولات الـ A6، بتعمل الـ activation + +88 +00:06:54,570 --> 00:06:58,850 +فبتحول الـ .. بتحول الـ adenylate cyclase بيحول الـ + +89 +00:06:58,850 --> 00:07:05,050 +ATP لـ cAMP لـ cAMP بيزود .. بيحيم الـ إفراز + +90 +00:07:05,050 --> 00:07:08,610 +للـ electrolytes حتى تسيط كميتها، حتى تخفيفها + +91 +00:07:08,610 --> 00:07:12,410 +الشثمة من الـ water ده الـ ice blockers، ده الـ ice + +92 +00:07:12,410 --> 00:07:14,970 +blockers، هاي .. هاي بالفعل، هاي الـ story نفس + +93 +00:07:14,970 --> 00:07:18,510 +القصة، عارفين ليش نفس القصة؟ لأن أنا هتكلم عن + +94 +00:07:18,510 --> 00:07:22,690 +التوكسين، الـ action تبعه، بنوصف واحد cholera toxin + +95 +00:07:23,410 --> 00:07:26,310 +الكوليراي توكسينه بتاعة الانتروتوكسينيك إيشريشيا + +96 +00:07:26,310 --> 00:07:30,070 +كوليراي will produce diarrhea similar to that of + +97 +00:07:30,070 --> 00:07:33,650 +Vibrio cholera نتكلم شوية عن Vibrio cholera لأنه + +98 +00:07:33,650 --> 00:07:36,090 +مهم جداً لإذ عرفنا المعلومات اللي على Vibrio + +99 +00:07:36,090 --> 00:07:37,710 +cholera هي نفس المعلومات اللي على الانتروتوكسينيك + +100 +00:07:37,710 --> 00:07:43,010 +إيشريشيا كولراي الـ diarrhea احنا قلنا في اللوحة أن + +101 +00:07:43,010 --> 00:07:47,490 +الـ stool is not associated with blood ما في شيء مش مدمم + +102 +00:07:57,110 --> 00:08:00,390 +أي دياريا في الـ rice water لما بنغسل الرز أشبه كله + +103 +00:08:00,390 --> 00:08:05,430 +المياه ملبّن و whitish صح ولا لأ؟ هناك + +104 +00:08:05,430 --> 00:08:09,050 +resemblance ما بين الدياريا وما بين الـ rice water + +105 +00:08:09,050 --> 00:08:13,610 +stool لذلك لما نتقف الناس في حالة الوباء بنقولهم + +106 +00:08:13,610 --> 00:08:17,570 +أن أي دياريا في حالة الوباء أي دياريا هي + +107 +00:08:17,570 --> 00:08:21,130 +suspected to be as a result of Vibrio cholera + +108 +00:08:26,750 --> 00:08:33,830 +Loose stool per day لكن لما يكون في rice water + +109 +00:08:33,830 --> 00:08:41,440 +stool هذا مدعاة لمشروع الطبيب أن يعالج المريض + +110 +00:08:41,440 --> 00:08:46,600 +على أنه كوليرا قبل ما يبدأ .. قبل ما يعني يكون فيه + +111 +00:08:46,600 --> 00:08:49,520 +تشخيص نهائي يبقى بيصير فيه أن أنا اتقال أن هو + +112 +00:08:49,520 --> 00:08:52,660 +المريض مصاب بكوليرا ليه احنا مهتمين بأننا نعالج + +113 +00:08:52,660 --> 00:08:57,300 +المريض بسرعة من جانب الجهد التشخيصي لحاجتين لمصلحة + +114 +00:08:57,300 --> 00:09:00,640 +المريض نفسه أن احنا بدنا .. لأن الـ .. الـ .. الـ .. + +115 +00:09:00,640 --> 00:09:01,020 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +116 +00:09:01,020 --> 00:09:01,360 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +117 +00:09:01,360 --> 00:09:01,820 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +118 +00:09:01,820 --> 00:09:02,100 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +119 +00:09:02,100 --> 00:09:04,340 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +120 +00:09:04,340 --> 00:09:04,520 +.. الـ + +121 +00:09:08,550 --> 00:09:13,590 +والمسألة الثانية عشان خاطر المجتمع هذا الشخص + +122 +00:09:13,590 --> 00:09:18,650 +المصاب يشكل reservoir للبكتيريا فمن واجبنا ندور + +123 +00:09:18,650 --> 00:09:23,110 +عليه وعلاجهم يعني حتى العائلة اللي بيكون فيها + +124 +00:09:23,110 --> 00:09:26,670 +فرد مصاب غالباً لازم نعمل لهم quarantine quarantine + +125 +00:09:26,670 --> 00:09:30,810 +يعني حجز حجر صحي عشان أنا أطلع مين منهم ممكن + +126 +00:09:30,810 --> 00:09:33,070 +يكون exposed أو ممكن أعطيه + +127 +00:09:36,940 --> 00:09:41,720 +إذا احنا معنّين لحظة rice water stool معنوعة صغيرة + +128 +00:09:41,720 --> 00:09:45,860 +طبعًا استخدمناها في نشر الوعي في حالة اللي هو + +129 +00:09:45,860 --> 00:09:51,060 +الأوبيا مش بس الطبيب بيقصر أو بتخلع ال protocol في + +130 +00:09:51,060 --> 00:09:53,680 +حالة الأوبيا احنا كمان بتخلع ال protocol في + +131 +00:09:53,680 --> 00:09:57,440 +المختبر كيف نتخلع ال protocol؟ هأعطيكم مثل الكورية + +132 +00:09:57,440 --> 00:10:03,320 +نفسيقررنا عينة زرعناها بعد 24 ساعة تطلع لمستعملها + +133 +00:10:03,320 --> 00:10:08,360 +صح؟ بعد 24 ساعة نطلع عليها ساكروس فرمنتر من طول + +134 +00:10:08,360 --> 00:10:11,280 +مش عارف شوه نعملها biochemical test كمان 24 ساعة + +135 +00:10:11,280 --> 00:10:17,060 +صح؟ هجدت صار الآن 48 ساعة و 24 ساعة أخرى + +136 +00:10:17,060 --> 00:10:21,620 +لستيرولوجي زائد ال sensitivity testing هي 72 ساعة + +137 +00:10:21,620 --> 00:10:25,310 +كاملة هل من المنطق أن في حالة الأوبيا أنتظر 48 + +138 +00:10:25,310 --> 00:10:29,370 +ساعة؟ طب أنا أُتَقَال كيف باكسل ال protocol بعد 24 + +139 +00:10:29,370 --> 00:10:32,510 +ساعة المستعمرة بتنمو suspected colonist إيه يعني + +140 +00:10:32,510 --> 00:10:35,110 +suspected colonist؟ مشترك بها، يلها مواصفات + +141 +00:10:35,110 --> 00:10:38,210 +معينة، بدل من بعملها biochemical test تجارب 24 + +142 +00:10:38,210 --> 00:10:42,770 +ساعة، بعملها فحص serology واحد مباشر أو فحصين one + +143 +00:10:42,770 --> 00:10:45,130 +minute test one minute test يعني ما بيستخدموا + +144 +00:10:45,130 --> 00:10:50,390 +معايا طويلة فبأقتصر من ال diagnosis حوالي 48 ساعة + +145 +00:10:51,370 --> 00:10:54,790 +هذا لا يعني أن أنا لا أكمل إجراءات روتينية لكن أنا + +146 +00:10:54,790 --> 00:10:58,470 +بهذا الحال بعمل تقريرين تقرير مبدئي وتقرير نهائي + +147 +00:10:58,470 --> 00:11:03,010 +حتى أُوفر وقت لأن احنا في حالة غير طبيعية اسمها + +148 +00:11:03,010 --> 00:11:08,130 +dehydration مكتر، لقيت فرازي أن هي ال electrolyte + +149 +00:11:08,130 --> 00:11:15,490 +ما بنحلها إلا بالناء يعني بالواتر يعني نتخلص من كمية + +150 +00:11:15,490 --> 00:11:20,290 +ال electrolyte بالناء صحيح يعني هينتج المشكلة + +151 +00:11:20,290 --> 00:11:24,590 +ثانية؟ ما هو طبعًا مشكلتين هينتج، حاسس أن هناك نقص + +152 +00:11:24,590 --> 00:11:28,610 +في المياه ونقص في الالكتروليات، لذلك أي قيمة + +153 +00:11:28,610 --> 00:11:34,640 +لبيارية should include two measures water + +154 +00:11:34,640 --> 00:11:38,160 +replacement اللي هو الـrehydration وعالطريقة المحلية + +155 +00:11:38,160 --> 00:11:42,260 +وال electrolyte بتسمعوا بالمصريين .. إخوان + +156 +00:11:42,260 --> 00:11:46,040 +المصريين اللطفين كان عندهم دعاية مشهورة لمعالجة + +157 +00:11:46,040 --> 00:11:52,700 +.. كان اسمه محلول معالجة الجفاف وجعلوا يمكن سنين + +158 +00:11:52,700 --> 00:11:59,540 +يعملوا عملية تسويق لهذا المنتج على التلفزيون لأنه + +159 +00:11:59,540 --> 00:12:01,660 +ديارية في العالم الثالث + +160 +00:12:06,060 --> 00:12:08,880 +هو قاتل رقم واحد وبالتالي احنا مضطرين بالفعل + +161 +00:12:08,880 --> 00:12:13,320 +نعالجه بأنه نرجع له المي اللي فقدها ونرجع له اللي هو + +162 +00:12:13,320 --> 00:12:17,100 +ال electrolyte اللي فقدها فجزء مهم جدا البعض + +163 +00:12:17,100 --> 00:12:21,100 +بيعتقد أن إيقاف الديارية هو أمر مهم لإيقاف + +164 +00:12:21,100 --> 00:12:25,540 +الديارية البعض بيظن أنه غير مهم وبالعكس قد يؤذي + +165 +00:12:25,540 --> 00:12:31,490 +المريض لذلك لا تستخدم الانفيا النهار اسمها ايموديوم + +166 +00:12:31,490 --> 00:12:34,590 +معروف عن أن هو هذا بيوقف حركة الدودة .. الحركة + +167 +00:12:34,590 --> 00:12:37,790 +الدودية اللي أمها هو هذا محدد ببتاعته خلال نص ساعة + +168 +00:12:37,790 --> 00:12:41,470 +وجه في الإسهال فبقى إذا احنا متفقين إن الإسهال هو + +169 +00:12:41,470 --> 00:12:44,950 +reaction طبيعي من الجسم للتخلص من ال micro + +170 +00:12:44,950 --> 00:12:48,690 +organism فبالتالي إذا وقفنا الإسهال بطريقة هذه + +171 +00:12:48,690 --> 00:12:53,380 +الميكروب والتوكسين هيبقى له موجود لكن الأفضل اللي + +172 +00:12:53,380 --> 00:12:57,240 +ما حكينا إن السوائل والإلكتروبات اللي بتفقدها + +173 +00:12:57,240 --> 00:13:01,660 +نعوضها والجانب الثاني أن يعالج العامل المسبب نتخلص + +174 +00:13:01,660 --> 00:13:05,260 +منه إذا كان بالبروكلية إذا كان سالمونيلا إذا كان + +175 +00:13:05,260 --> 00:13:08,020 +إشريشية إذا كان شيجلة إذا كان انتاميبة أو whatever + +176 +00:13:08,020 --> 00:13:12,620 +causes it احنا خرجنا عن النص كثير لكن كله عشان + +177 +00:13:12,620 --> 00:13:13,620 +نوضع نقطة واحدة + +178 +00:13:21,760 --> 00:13:26,740 +و it causes conditions similar to cholera بسبب + +179 +00:13:26,740 --> 00:13:29,840 +الخط العزيزي هنا دياريا like cholera زي ما حكينا + +180 +00:13:29,840 --> 00:13:34,820 +milder أخف شوية من ال cholera لأن ال growth و ال + +181 +00:13:34,820 --> 00:13:38,060 +multiplication تبع الإشريشيا الcholera أقل درجة من + +182 +00:13:38,060 --> 00:13:42,280 +مين من ال vibrio cholera ممكن تتشر في الحضانات + +183 +00:13:42,280 --> 00:13:45,880 +يعني في الأطفال اللي عايشين في الحضانات أو أول + +184 +00:13:45,880 --> 00:13:48,000 +حاجة بيسموها travelers يعني ال travelers يعني يعني + +185 +00:13:48,000 --> 00:13:51,380 +المعروف عن إسهال المسافرين الذي ينتقل من مكان إلى + +186 +00:13:51,380 --> 00:13:56,020 +مكان وتتغذى عليه الطبيعة الجو والغذاء ممكن يصاب + +187 +00:13:56,020 --> 00:14:02,800 +بهذه الانفكار caused by LT ST أو both LT and ST LT + +188 +00:14:02,800 --> 00:14:06,760 +اللي هو ال bio-toxin وهو اللي يشبه الكوليرا توكسين + +189 +00:14:06,760 --> 00:14:13,120 +LT توكسين والST stable toxin يعني فكرة كلمة + +190 +00:14:13,120 --> 00:14:14,220 +الفايروس stable + +191 +00:14:17,150 --> 00:14:21,310 +إذا ال L-T و L-S-T توكسين اللي بيشتغله بالطريقة + +192 +00:14:21,310 --> 00:14:25,570 +التابعة ال heat-loving toxin like cholera gene أو + +193 +00:14:25,570 --> 00:14:28,670 +cholera toxin it activates adenylate cyclase + +194 +00:14:28,670 --> 00:14:33,190 +enzyme بيرفع من ال cyclic AMP بيزيد من ال + +195 +00:14:33,190 --> 00:14:38,620 +secretions لل water ions الـ heat stable toxin + +196 +00:14:38,620 --> 00:14:42,780 +بيشبه الأولان تمامًا في الميكانيزم بس بدل ما يشتغل + +197 +00:14:42,780 --> 00:14:46,620 +على قدرات cyclase enzyme بيشتغل على جوانالات اللي + +198 +00:14:46,620 --> 00:14:49,800 +هو من الجوانين جوانالات cyclase enzyme بيسيطر على + +199 +00:14:49,800 --> 00:14:55,040 +activation بيزيد كمية الـ cyclic GMP بدل ما يزيد + +200 +00:14:55,040 --> 00:14:58,260 +ال secretion ممنوع ال absorption ماهي نفس المحصلة + +201 +00:14:58,260 --> 00:15:02,360 +ده إذا منعنا الabsorption .. ال + +202 +00:15:04,570 --> 00:15:09,590 +أه فبيمنع ال .. ال .. ال ions من أنها تمتص وبتاع + +203 +00:15:09,590 --> 00:15:12,190 +اللي بيصير فيه كمية كبيرة من ال ions والماء في + +204 +00:15:12,190 --> 00:15:18,850 +الداخل إذن ال heat level بيشبه لحد كبير ال heat + +205 +00:15:18,850 --> 00:15:22,550 +stable في النتيجة في المحصلة لكن they work + +206 +00:15:22,550 --> 00:15:25,010 +different واحد بيشتغل على ال analyte واحد بيشتغل + +207 +00:15:25,010 --> 00:15:27,790 +على ال gonalate هذا واحد واحد بيزيد ال secretion + +208 +00:15:27,790 --> 00:15:33,370 +واحد بيقلل من ال absorption وهي واحدة اللي بيقدر أو + +209 +00:15:33,370 --> 00:15:36,010 +بيزيد في الآخر بيصير فيه accumulation ل ال + +210 +00:15:36,010 --> 00:15:40,450 +electrolyte في الجسم هذه نفس المعلومات اللي بتوري + +211 +00:15:40,450 --> 00:15:43,310 +اللي هو ال toxin LT و ST كيف بيشتغلوا على اللي + +212 +00:15:43,310 --> 00:15:46,890 +اللي هو ال Y طبعًا بتورينا كيف اشتراك بالله ال + +213 +00:15:46,890 --> 00:15:51,590 +toxin في امرأة microbe بتير مهم في الإشريشية كلها + +214 +00:15:51,590 --> 00:15:53,250 +اسمه entero invasive + +215 +00:15:56,200 --> 00:16:00,200 +يعني حتى المصاميات هذه فيها على شوية خلافات، بمعنى + +216 +00:16:00,200 --> 00:16:03,480 +أنه قبل نجد أن واحد بيقول عنها intro-hemorrhagic، + +217 +00:16:03,480 --> 00:16:08,730 +وواحد بيقول عنها intro-invasive في مبرر لل .. لل .. + +218 +00:16:08,730 --> 00:16:14,650 +لل .. يعني لعدم الموضوع هذا إيش المبرر؟ أن بعض ال + +219 +00:16:14,650 --> 00:16:19,170 +strains بيمتلك صفتين أو أكثر قد تشتركها مع بكتيري + +220 +00:16:19,170 --> 00:16:22,230 +أخرى وتنفصل عن بكتيري أخرى فبيصير فيهم الاشتباه + +221 +00:16:22,230 --> 00:16:26,250 +فيها ده أو خلينا نقول التباس في تسمية الميكروب لأ + +222 +00:16:26,250 --> 00:16:31,310 +احنا قدرنا نص اسمه بقول enteroinvasive Escherichia + +223 +00:16:31,310 --> 00:16:34,350 +coli هذا الميكروب عنده المفضل على أنه يعمل + +224 +00:16:34,350 --> 00:16:35,930 +attachment لـ الميكروب + +225 +00:16:40,300 --> 00:16:43,600 +وال بيولايت أقول عنها مكون أساسي في ال E.coli حتى + +226 +00:16:43,600 --> 00:16:47,160 +اللي بتسبب بيورنايترات infection لأن كيف بتعمل + +227 +00:16:47,160 --> 00:16:51,740 +بيوليفريتس عكس طيار صح؟ لازم يكون مباطلة هي على + +228 +00:16:51,740 --> 00:16:59,240 +الالتصاق ال outer membranes بتاعت البكتيريا بتعمل + +229 +00:16:59,240 --> 00:17:05,300 +عملية modification في ال cell membrane تبع الخلايا + +230 +00:17:05,300 --> 00:17:08,920 +اللي هو ال cell وبتخلي البكتيريا تعملها + +231 +00:17:08,920 --> 00:17:13,150 +phagocytosis الآن البكتيريا بالضبط البكتيريا ساعة + +232 +00:17:13,150 --> 00:17:17,410 +واحد موجودة الآن جوا الخلايا ال intestinal cells + +233 +00:17:17,410 --> 00:17:22,950 +بتجي وتعمل destruction للخلايا عبر ال protein + +234 +00:17:22,950 --> 00:17:26,330 +synthesis inhibition فاكرين الشيجيلاتوكسين؟ + +235 +00:17:26,330 --> 00:17:31,210 +الشيجيلاتوكسين عشان بيشتغل بالضبط عبر ال ribosome + +236 +00:17:31,210 --> 00:17:35,130 +وبيوقف ال protein synthesis فال S لاحظتوا إيش آخر + +237 +00:17:35,130 --> 00:17:39,010 +جملة بتقولها هنا؟ it resembles .. يعني المعنى اللي + +238 +00:17:39,010 --> 00:17:42,310 +حاكيه الأولانية اللي جاب لها كان resemble كلها وهاد + +239 +00:17:42,310 --> 00:17:45,450 +resemble شيجلة وهاد الكلام اللي بقى أكد عليه .. + +240 +00:17:45,450 --> 00:17:51,150 +اللي أكد عليه المحاضرة السابقة أن في transfer of + +241 +00:17:51,150 --> 00:17:55,530 +information من genus ال Vibrio للإشريشية من genus + +242 +00:17:55,530 --> 00:18:00,650 +الشيجلة للإشريشية وبالتالي في gene transfer أو على + +243 +00:18:00,650 --> 00:18:04,210 +الأقل هذا مؤشر أن في gene transfer بين genus و + +244 +00:18:04,210 --> 00:18:06,470 +genus طيب + +245 +00:18:06,470 --> 00:18:13,770 +البكتريولوجي هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو هو + +246 +00:18:14,900 --> 00:18:17,260 +يعني ما فيش داعي أن تتكاثر وتطلع بعض الخلية و + +247 +00:18:17,260 --> 00:18:21,100 +تطلع خلية ثانية، لأ من خلية إلى خلية، بنسميها + +248 +00:18:21,100 --> 00:18:26,820 +horizontal تمام؟ أو lateral symptoms include + +249 +00:18:26,820 --> 00:18:31,000 +fever، هنفسر ال symptoms من وين اجت في غالب + +250 +00:18:31,000 --> 00:18:33,760 +الأحيان، fever، severe abdominal cramps اللي هي + +251 +00:18:33,760 --> 00:18:34,460 +مغض شديد + +252 +00:18:47,670 --> 00:18:52,690 +هو عبارة عن ميوكاس and pus and blood، ممكن يكون + +253 +00:18:52,690 --> 00:18:57,970 +كمان إيه؟ blood فقط، لكن لإن الإشريكية frequent، + +254 +00:18:57,970 --> 00:19:03,070 +مرارة كراية، مافيش حاجة، اسمها stool الآن، فقط + +255 +00:19:03,070 --> 00:19:06,970 +الناتج هو الميوكاس والخلايا الميتة والبكتيريا + +256 +00:19:06,970 --> 00:19:11,670 +والصديد اللي بينتج، هنعرف من كل واحد منهم من وين + +257 +00:19:11,670 --> 00:19:17,820 +إيه it resembles villus بتاعنا من وين اجت هاي ال + +258 +00:19:17,820 --> 00:19:22,520 +.. ال intestinal wall هاي ال microvilli ال + +259 +00:19:22,520 --> 00:19:26,760 +microvilli عليها ال mucus هاي البكتيريا عشان تلتصق + +260 +00:19:26,760 --> 00:19:31,420 +لازم تزيح الـ mucus و تلتصق بمين؟ بالبكتيريا بال .. + +261 +00:19:31,420 --> 00:19:34,200 +بالـ microvilli الآن مش بحاجة لحاجة إن الـ mucus + +262 +00:19:34,200 --> 00:19:38,800 +انزعج إنه راح لأ موجود لكن في مكان قريب الآن + +263 +00:19:38,800 --> 00:19:42,000 +البكتيريا دخلت جوا و موضت في الخلية الأولى و + +264 +00:19:42,000 --> 00:19:44,260 +التانية و التالتة و الرابعة و الخمسة أعلى تسطر من + +265 +00:19:44,260 --> 00:19:47,480 +الخلايا اللي موضتهم الخلايا بيختفوش عن وجه الأرض + +266 +00:19:47,480 --> 00:19:53,400 +فجأة لكن بيقولهم ميتين الاسم بيتخلص منهم و بيصيلهم + +267 +00:19:53,400 --> 00:19:57,240 +sloughing sloughing يعني ايه؟ يعني بيتقشر في + +268 +00:19:57,240 --> 00:20:01,040 +المعمل الآن بينزلوا و منزلوا في الـ intestinal + +269 +00:20:01,040 --> 00:20:04,480 +lumen في اللي من في التجريب ايش عليهم الخلايا + +270 +00:20:04,480 --> 00:20:07,900 +دولة؟ هم epithelial cells ميتة فيهم بكتيريا و عليهم + +271 +00:20:07,900 --> 00:20:13,330 +ميوكاس يبقى الـ mucus اللى نزل فى الـ stool نادى عن + +272 +00:20:13,330 --> 00:20:17,630 +مين؟ عن موت الخلايا و سقوطها، يبقى بتوقع علاج فى + +273 +00:20:17,630 --> 00:20:21,390 +الـ stool mucus ذات Epithelial cells ذات البكتيريا + +274 +00:20:21,390 --> 00:20:24,950 +نفسها ليها جرام نيجاتيف بصلاي أو باندنطلي، يعني + +275 +00:20:24,950 --> 00:20:25,970 +بشكل كبير + +276 +00:20:34,860 --> 00:20:37,400 +هى اللى بتاخد الـ nutrient اللى ساعد على ال + +277 +00:20:37,400 --> 00:20:41,680 +absorption صح من الـ micro villi بتاخدها و بتوزعها + +278 +00:20:41,680 --> 00:20:45,280 +على الرسم يبجى على طول ورا الـ villi هيكون فيه + +279 +00:20:45,280 --> 00:20:49,380 +capillaries فهذه مصنوعة من PVC ولا plastic ولا ايش + +280 +00:20:49,380 --> 00:20:55,260 +مصنوعة مش برضه خلايا مش epithelial cells البكتيريا + +281 +00:20:55,260 --> 00:20:58,860 +بتتسخ فيها و بتعملها destruction يبجى الـ blood + +282 +00:20:58,860 --> 00:21:01,700 +هيسير او الـ capillaries هيسير لها rupture يعني + +283 +00:21:01,700 --> 00:21:06,930 +هتنفجر يبجى هينزلدم في الـ stool يعني في عند ال + +284 +00:21:06,930 --> 00:21:10,870 +blood و في عند الـ mucus طب إيش اللي جاب الصديد؟ + +285 +00:21:10,870 --> 00:21:16,630 +كلكوا بتعرفوا إن خليانة عندما تموت تصرخ تصرخ لتنبه + +286 +00:21:16,630 --> 00:21:19,470 +جهاز المنع الصراخ تبعها عبارة عن cytokines + +287 +00:21:19,470 --> 00:21:24,210 +messengers طبعا؟ ابتدي حضرة ال .. ال .. ال .. ال + +288 +00:21:24,210 --> 00:21:24,730 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +289 +00:21:24,730 --> 00:21:25,210 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +290 +00:21:25,210 --> 00:21:25,290 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +291 +00:21:25,290 --> 00:21:26,870 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +292 +00:21:26,870 --> 00:21:27,130 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +293 +00:21:27,130 --> 00:21:27,950 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +294 +00:21:27,950 --> 00:21:28,490 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +295 +00:21:28,490 --> 00:21:32,500 +ال .. ال ..الإشريكية معروفة عنها خدوا بالكوا انها can + +296 +00:21:32,500 --> 00:21:36,740 +exist intracellular مش سهل نقتلها بمين؟ بالـ + +297 +00:21:36,740 --> 00:21:41,600 +phagocytes فكان مينية كبيرة من الخلايا اللي جاية + +298 +00:21:41,600 --> 00:21:48,420 +تحارب بتموت من مين؟ من الإشريكية وبالتالي موت الخلايا + +299 +00:21:48,420 --> 00:21:52,660 +اللي هي الـ phagocytes بينتج عنه صديد الصديد هو الـ + +300 +00:21:52,660 --> 00:21:52,960 +pus + +301 +00:21:56,430 --> 00:22:00,170 +بتا هي عبارة عن ايه؟ عن white blood cell ماتة اللي + +302 +00:22:00,170 --> 00:22:05,550 +هي الـ neutral field إذا انفسرنا نزول الـ pus، نزول + +303 +00:22:05,550 --> 00:22:09,770 +الدم، نزول الـ mucus، نزول الـ epithelial cells، + +304 +00:22:09,770 --> 00:22:12,990 +نزول كمية كبيرة من اللي جراها، جرام نيجاتيف بس الله، + +305 +00:22:12,990 --> 00:22:16,730 +بالإضافة إلى الديارة، ما فسرناها خلاص الديارة، + +306 +00:22:16,730 --> 00:22:20,150 +مافيش epithelial cells تنفس، accumulation ديارة + +307 +00:22:21,290 --> 00:22:24,450 +بصرة الصراحية يبقى معنا بحكي أنه هذه البكتيريا + +308 +00:22:24,450 --> 00:22:28,710 +بتعمل إشريكية like يبقى بنقدر نقول عنها dysentery + +309 +00:22:28,710 --> 00:22:33,570 +كلمة dysentery هي مميزاتها أنه فيها عندى stool، + +310 +00:22:33,570 --> 00:22:39,410 +دياريا، دياريا فيها blood، فيها mucus وفيها blood + +311 +00:22:39,410 --> 00:22:44,650 +و mucus بص و بالظبط بص و طبعا بالله فانا انا اقول + +312 +00:22:44,650 --> 00:22:50,760 +ان انا epithelial sexDysentery,Dysentery,Dysentery + +313 +00:22:50,760 --> 00:22:54,840 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +314 +00:22:54,840 --> 00:22:56,600 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +315 +00:22:56,600 --> 00:22:57,000 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +316 +00:22:57,000 --> 00:22:57,060 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +317 +00:22:57,060 --> 00:23:06,480 +,Dysentery + +318 +00:23:06,480 --> 00:23:06,560 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +319 +00:23:06,560 --> 00:23:10,560 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +320 +00:23:10,560 --> 00:23:14,220 +,Dysentery + +321 +00:23:15,170 --> 00:23:18,330 +فى برايل يعني مافيش حرارة، مافيش سخونة عند المرض + +322 +00:23:18,330 --> 00:23:23,270 +يكون أحد أهم أخطر الـ conditions اللى بتسببها هذه + +323 +00:23:23,270 --> 00:23:27,690 +البكتيريا هي ما يسمى بالـ HUS Hemolytic Uremic + +324 +00:23:27,690 --> 00:23:30,510 +Syndrome + +325 +00:23:32,270 --> 00:23:35,210 +وانا فجأة فانده مطورة لإنه انا باعتقادي ان هو + +326 +00:23:35,210 --> 00:23:38,550 +الميكروب يعني نوع ما مهمل في قطر غزة على الرغم من + +327 +00:23:38,550 --> 00:23:43,450 +انه الدراسات اللي أجريت محليا أثبتت وجود الميكروب + +328 +00:23:43,450 --> 00:23:47,690 +في مرضى و أثبتت وجود الميكروب في عينات غذائية منها + +329 +00:23:47,690 --> 00:23:53,000 +اللحم، الحوم، منها الدواجن، منها الخضروات اللي تأكل + +330 +00:23:53,000 --> 00:23:57,960 +طازجة بمعناه انه ماتتعرضش لحرارة عالية فالبكتيريا + +331 +00:23:57,960 --> 00:24:02,700 +موجودة لكن الكشف عنها للأسف يعني عليها الاستفادة + +332 +00:24:02,700 --> 00:24:08,560 +مستشفياتنا ماتتجمش بالواقع في هذا الإطار لذلك هناك + +333 +00:24:08,560 --> 00:24:16,660 +بعض الأطفال قد نفقدهم بسبب فشل كله ناتج عن الإصابة + +334 +00:24:16,660 --> 00:24:23,130 +بهذا الميكروب بيُعطى مريض أنتبايتك فجأة اللي ما يخف + +335 +00:24:23,130 --> 00:24:28,330 +بتزيد الحالة عنده، ليش؟ لأن الخلايا لما تموت بتزيد + +336 +00:24:28,330 --> 00:24:31,930 +الـ destruction اللي موجود طب احنا مش عارفين السبب + +337 +00:24:31,930 --> 00:24:34,610 +وقبلتها اللي بيجرب و انا انت بيتك واحد و اتنين و + +338 +00:24:34,610 --> 00:24:38,350 +تلاتة و اربع و للأسف ممكن يموت زي ما حكينا من + +339 +00:24:38,350 --> 00:24:43,410 +kidney failure و طفل kidney failure ناتجة عن + +340 +00:24:43,410 --> 00:24:46,270 +الإشريكية كولا اللي سمينها in terror يمرجك إشريكية + +341 +00:24:46,270 --> 00:24:51,090 +كولا بتعرفوا الطعام التمييز بينها بتعمل hemolytic + +342 +00:24:51,090 --> 00:24:53,650 +anemia وبتعمل thrombocytopenia اللي اللي هو + +343 +00:24:53,650 --> 00:25:02,670 +platelet camp تسمعوا بالاسم هدا O157H7 + +344 +00:25:02,670 --> 00:25:07,570 +هي الـ endo hemolytic E.coli هي نفسها الـ E.coli + +345 +00:25:07,570 --> 00:25:12,910 +O157H7 اللي سمى في بريطانيا وامريكا hamburger + +346 +00:25:12,910 --> 00:25:14,510 +basselie او burger basselie + +347 +00:25:17,420 --> 00:25:22,940 +أول حالات تم اكتشافها للإيصال على الميكروب روبطت ب + +348 +00:25:22,940 --> 00:25:28,600 +under cooked hamburger under cooked يعني مش مطهي + +349 +00:25:28,600 --> 00:25:32,080 +جيدا و انا بقولكوا شغل في موضوع الطهي جيدا و مش + +350 +00:25:32,080 --> 00:25:37,820 +جيدا لأن عندهم هناك طبعا احنا .. اش .. اش العادة + +351 +00:25:37,820 --> 00:25:40,720 +الغذائية عندنا لما بنطلع رحلة مثلا بناخد نشوي على + +352 +00:25:40,720 --> 00:25:45,530 +البحر او في الخلاء مثلا او في منطقة و لا لا نشويهم + +353 +00:25:45,530 --> 00:25:48,770 +اكتر اشي بيعملوا بيعملوا اقراص همبرجر برضه + +354 +00:25:48,770 --> 00:25:54,050 +بيشوفوها فالان و جهزولهم ترمومترات قص الهمبرجر و + +355 +00:25:54,050 --> 00:25:57,550 +نحطوا فيه ترمومتر من جوا عشان نتأكل اذا اتحارست 75 + +356 +00:25:57,550 --> 00:26:01,870 +درجة او ايه عشان خاطر .. عشان خاطر نطمئن ان هذه + +357 +00:26:01,870 --> 00:26:07,330 +البكتيرا تكون ايه ماتة فبعند ال .. ال .. ال .. ال + +358 +00:26:07,330 --> 00:26:13,120 +ground beef او الـ ground meat اللحم المفروم بيحتاجش + +359 +00:26:13,120 --> 00:26:16,180 +لحرارة عالية عشان ينتهي وانتوا بتعرفوا الفرق بين + +360 +00:26:16,180 --> 00:26:19,580 +انتوا معارف ال .. في الأمور هذه و بتعرفوا الفرق + +361 +00:26:19,580 --> 00:26:23,600 +بين طغي لحم مفروم وغير مفروم تمام؟ هذا ليه استغرف + +362 +00:26:23,600 --> 00:26:27,180 +وقت؟ وانا مستغرف وقت فبتعرف مادام مابستغرقش وقت دي + +363 +00:26:27,180 --> 00:26:32,200 +بالحرارة مابتصلش للمنطصف ب .. ب .. ب .. ب .. بدرجة + +364 +00:26:32,200 --> 00:26:35,020 +كتلة انها تكون قتلة ايه؟ الميكروبات وبعدين بنقول + +365 +00:26:35,020 --> 00:26:39,240 +undercooked undercooked meat ممكن يكون سبب الأسباب + +366 +00:26:39,240 --> 00:26:45,560 +انتقال هذا الـ micro organism في كلمة شركة تانية اسمها + +367 +00:26:45,560 --> 00:26:52,120 +verotoxin و برضه هم عطيلة اسمها شركة like لإن عشان + +368 +00:26:52,120 --> 00:26:55,820 +انا اقولكوا في خلط ما بين الـ entero invasive و الـ + +369 +00:26:55,820 --> 00:27:00,210 +entero hemorrhage إشان الفيروتوكسين هنبدا أشرح و + +370 +00:27:00,210 --> 00:27:03,270 +هننهي في محالتنا عشان أعطيكوا فرصة كويسة أنه تلتقط + +371 +00:27:03,270 --> 00:27:06,710 +الأنفاس للاختبار ان شاء الله اليوم الفيروتوكسين + +372 +00:27:06,710 --> 00:27:10,810 +سمى الفيروتوكسين لإنه له تأثير على فيروس الـ + +373 +00:27:10,810 --> 00:27:14,310 +culture إشان الفيروس الـ culture نرجع لأفريقيا و + +374 +00:27:14,310 --> 00:27:18,970 +نروح على الغابات و نطلع على الشجرة و نلاقي قرد + +375 +00:27:18,970 --> 00:27:24,870 +أخضر اسمه African green monkey kidney أفضل African + +376 +00:27:24,870 --> 00:27:28,910 +الـ African Green Monkey هدولة أخدنا + +377 +00:27:28,910 --> 00:27:32,230 +منهم kidney cells يعني أخدنا الـ kidney تبعتهم و + +378 +00:27:32,230 --> 00:27:35,890 +أخدنا منها خلايا و عملنا منها cell culture cell + +379 +00:27:35,890 --> 00:27:41,190 +line cell line اسمه Vero cell line يبقى الـ Vero من + +380 +00:27:41,190 --> 00:27:45,370 +عندي هي من اسم الـ cell culture اللي أخدناها من الـ + +381 +00:27:45,370 --> 00:27:49,370 +African Green Monkey تمام؟ أخدناها من عندي؟ من الـ + +382 +00:27:49,370 --> 00:27:54,050 +kidneys يبقى أنا اللي عندي Vero cell culture خونوا + +383 +00:27:54,050 --> 00:27:56,690 +بالكوا إنّ انتوا الانطباع اللي بتعرفوه على الـ cell + +384 +00:27:56,690 --> 00:28:01,210 +culture إنّه بنستخدمها لدراسة مين؟ للمكتيريا؟ لأ + +385 +00:28:01,210 --> 00:28:06,330 +cell culture، للفيروسات، الـ cell culture بننمّي + +386 +00:28:06,330 --> 00:28:08,870 +فيها الفيروسات، احنا بننمّي البكتيريا على + +387 +00:28:08,870 --> 00:28:11,810 +الـ medium، أنا بسمّيها artificial media، مظبوط هذه + +388 +00:28:11,810 --> 00:28:13,890 +cell culture، هذه لمين؟ للفيروسات + +389 +00:28:17,450 --> 00:28:22,150 +من ضمن هذه الأغراض إنّه أشوف مواد سامة تأثير مواد + +390 +00:28:22,150 --> 00:28:26,150 +سامة على الخلايا طبعًا الخلايا لما احنا بناخد من + +391 +00:28:26,150 --> 00:28:32,130 +كِدني و بنزرعها في المعمل تطلعش كِدني علمًا تطلع ما + +392 +00:28:32,130 --> 00:28:36,650 +يسمّى بـ mono layer فبقعه من الخلايا على السطح اللي + +393 +00:28:36,650 --> 00:28:41,150 +احنا زرعناها عليها mono layer فبقعه واحده تحت + +394 +00:28:41,150 --> 00:28:46,060 +المايكروسكوب نقدر نشوف الخلايا بشكل واضح وكل نوع من + +395 +00:28:46,060 --> 00:28:51,640 +الخلايا إله شكله المميز تبعه وكل فيروس أو كل مركب + +396 +00:28:51,640 --> 00:28:54,820 +بيعمل destruction برضه ممكن يكون الـ destruction + +397 +00:28:54,820 --> 00:29:01,760 +ميزة تميّز الفيروس بمعنى بعض الفيروسات بتعمل + +398 +00:29:01,760 --> 00:29:07,320 +lysis للخلايا بتدمرها بعض الفيروسات بتجمع الخلايا + +399 +00:29:07,320 --> 00:29:10,680 +أربعة أو خمسة في خلية واحدة، بيسمّوها syncytia، + +400 +00:29:10,680 --> 00:29:14,380 +طبعًا؟ فكل فيروس أو كل مُركّب له حاجة بيسمّوها + +401 +00:29:14,380 --> 00:29:19,020 +cytopathic effect، تأثيرات باطولوجية على الخلايا + +402 +00:29:19,860 --> 00:29:23,640 +لما جبنا الـ toxin تبع الـ E.coli اللي هي الـ + +403 +00:29:23,640 --> 00:29:27,300 +enterohemorrhagic حطيناها على الـ virus عملت + +404 +00:29:27,300 --> 00:29:31,880 +destruction، فما يسمّيناه فيرو توكسين، يعني سام + +405 +00:29:31,880 --> 00:29:35,340 +للخلايا الفيرو، عرفنا إيه الفيرو؟ عرفنا إيه؟ على + +406 +00:29:35,340 --> 00:29:42,770 +آخره، بتنتج أيضًا hemolysins غالبًا بتصيب children + +407 +00:29:42,770 --> 00:29:46,430 +younger than 5 years old وبتسبّب Hemorrhagic + +408 +00:29:46,430 --> 00:29:49,990 +Colitis Hemorrhagic Colitis يعني التهاب في القضاء + +409 +00:29:49,990 --> 00:29:54,490 +ونزف يكون فيه نزف نكمل إن شاء الله المرة القادمة + +410 +00:29:54,490 --> 00:29:56,810 +بعد اللي هو الامتحان diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..aa6cdbac52f1d7510ba718b6b76e3868a4d935bc --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY_postprocess.srt @@ -0,0 +1,1640 @@ +1 +00:00:20,590 --> 00:00:24,930 +بسم الله الرحمن الرحيم استكمل ان شاء الله موضوع + +2 +00:00:24,930 --> 00:00:28,990 +الـ Gram Negative Bacilli الـ Enterobacteriaceae + +3 +00:00:28,990 --> 00:00:35,070 +بدأنا بالمشهورة الاشريشيا كولاي تحدثنا عن + +4 +00:00:35,070 --> 00:00:37,770 +الاشريشيا كولاي على إنها normal microbiota of the + +5 +00:00:37,770 --> 00:00:41,070 +intestinal tract و تحدثنا عنها إنها outside the + +6 +00:00:41,070 --> 00:00:44,110 +intestinal tract is apoptotic و هي المثال عليها + +7 +00:00:44,110 --> 00:00:46,770 +اللي قُلتها على اللوحة إنها تعمل urinary tract + +8 +00:00:46,770 --> 00:00:49,170 +infection بالعكس هي number one cause of urinary + +9 +00:00:49,170 --> 00:00:49,990 +tract infection + +10 +00:01:01,000 --> 00:01:08,040 +الان انا لما اخدت عينة اليوران فى المعمل وقررت انه + +11 +00:01:08,040 --> 00:01:12,400 +positive positive يعني فى growth لبكتيريا وكانت + +12 +00:01:12,400 --> 00:01:15,420 +ايشريشيا كولاي كان ي differentiate between + +13 +00:01:15,420 --> 00:01:19,800 +cystitis and pyronephritisبتقدروا تمييزوا بينت + +14 +00:01:19,800 --> 00:01:23,820 +انت؟ صعب جدا لنميز بينت انت من عينة UL المصلطة على + +15 +00:01:23,820 --> 00:01:27,120 +المختبر، واحنا ماعناش معلومات إلا إن هو عينة UL، + +16 +00:01:27,120 --> 00:01:29,940 +لأنه إذا كان بيوليوفريتس أو سيستريتس، هتطلع + +17 +00:01:29,940 --> 00:01:30,900 +العيناها + +18 +00:01:33,200 --> 00:01:38,260 +لأ، بدى اوضح نقطة القاعدة هنا، انه في limitations، + +19 +00:01:38,260 --> 00:01:43,120 +ايه ال limitations؟ يعني حدود، محددات لحت رتائبنا + +20 +00:01:43,120 --> 00:01:46,920 +في المزرعة، البعض مننا بيظن انه إذا عزل مايكروب، + +21 +00:01:46,920 --> 00:01:50,600 +فهو هذا هو المسبب لل infection، إذا عزل مايكروب، + +22 +00:01:50,600 --> 00:01:53,920 +فهو هذا هو ال disease، لأ الحقيقة أن هناك عامل أخر + +23 +00:01:53,920 --> 00:01:58,280 +لازم نظر إليها بالاتباع، مثلاللتفريق ما بين + +24 +00:01:58,280 --> 00:02:02,500 +cestitis و pyruhnephritis نضطر نعمل فحص اسمه CRP + +25 +00:02:02,500 --> 00:02:05,820 +اللي وزير active protein هتكلم عنه في مرحلة أخر في + +26 +00:02:05,820 --> 00:02:06,940 +ال micro pyruh + +27 +00:02:18,470 --> 00:02:21,890 +كانت المثال التانى على ان الاشريشيا كوراي outside + +28 +00:02:21,890 --> 00:02:24,550 +the intestinal tract هي pathogen السبسيز و ال + +29 +00:02:24,550 --> 00:02:27,770 +meningitis و خاصة في ال neonatal و برجه ذكرنا + +30 +00:02:27,770 --> 00:02:30,970 +معلومة ان هي number one cause of neonatal + +31 +00:02:30,970 --> 00:02:35,250 +meningitis بس أكتنا على حقيقة ان ال strains اللى + +32 +00:02:35,250 --> 00:02:40,130 +بتسبب neonatal meningitis غالبا بتكون capsulated و + +33 +00:02:40,130 --> 00:02:43,050 +عندها K1 انتجين و اللى بيقول عنه هو ال capsular + +34 +00:02:43,050 --> 00:02:48,460 +antigenالان انتقلنا لمرحلة انهم outside the + +35 +00:02:48,460 --> 00:02:50,500 +intestinal tract وانتقلنا لقاله mainly ل inside + +36 +00:02:50,500 --> 00:02:53,480 +the intestinal tract احنا اتفقنا انه في اربع من ال + +37 +00:02:53,480 --> 00:02:57,300 +bacterial C احكي التلاتة الاولين فاكر اني اقول + +38 +00:02:57,300 --> 00:03:02,640 +اشريشيا كولاي وقلنا سالمونيلا شيجيلا ويرسينيا هدول + +39 +00:03:02,640 --> 00:03:04,960 +التلاتة they are not normal in the intestinal + +40 +00:03:04,960 --> 00:03:07,700 +tract they cause intestinal tract infection ضفنا + +41 +00:03:07,700 --> 00:03:10,880 +اليهم في المرة التانية الاشريشيا كولاي وقلنا some + +42 +00:03:10,880 --> 00:03:13,180 +strains مش كلهم some strains + +43 +00:03:17,960 --> 00:03:21,780 +طب نحط شق أمام ال E cherisha coli مثلا قدامي على + +44 +00:03:21,780 --> 00:03:27,200 +لوحة بالبول انترو فاطو جنك E cherisha coli طبعا + +45 +00:03:27,200 --> 00:03:32,580 +هذا الاسم خادع الصحيح و بدنا نفكس عليه شوية من أي + +46 +00:03:32,580 --> 00:03:36,680 +ناحية خادع انه بيعطيني حق ان انا بتكلم عن كل ال E + +47 +00:03:36,680 --> 00:03:41,150 +cherisha coliاللي بتعمل infection في ال intestine + +48 +00:03:41,150 --> 00:03:44,830 +لإن ال pathogenic كان ممرضا لكن وقع الأمر هو + +49 +00:03:44,830 --> 00:03:49,390 +المصطلح اخترع للتعبير عن ما أنا قلته الآن لكن + +50 +00:03:49,390 --> 00:03:54,370 +لجينا أصناف مختلفة فخلينا واحد من الأصناف بالاسم + +51 +00:03:54,370 --> 00:03:57,530 +نفسه enteropathogenic واخترعنا أسماء جديدة من + +52 +00:03:57,530 --> 00:04:01,490 +الأسماء لأنواع أخرى هنشوفهم كمان شوية ف + +53 +00:04:01,490 --> 00:04:08,070 +enteropathogenic-epec هي أحد الأصناف و ليس كل + +54 +00:04:08,070 --> 00:04:13,490 +الأصنافتعمل fever, infandiaria, vomiting, nausea, + +55 +00:04:14,030 --> 00:04:17,610 +non bloody diarrhea, non bloody stool يعني ال + +56 +00:04:17,610 --> 00:04:23,430 +stool مش مدمّم، مافيش فيه، مش مصحوب بيه دم ناتج + +57 +00:04:23,430 --> 00:04:28,230 +غالبا عن destruction of surface microvilli تركزوا + +58 +00:04:28,230 --> 00:04:32,370 +معاه و تذكروا ال intestinal tract ال intestinal + +59 +00:04:32,370 --> 00:04:36,170 +tract فيها ال villi و ال villi هي المسؤولة عن ال + +60 +00:04:36,170 --> 00:04:38,790 +absorption لل digested food + +61 +00:04:45,880 --> 00:04:49,000 +طب انا ماتوقعش ان يكون destruction كامل لكل ال + +62 +00:04:49,000 --> 00:04:52,060 +microbial فبيقل ال absorption لما يقل ال + +63 +00:04:52,060 --> 00:04:58,220 +absorption ايش بيزيد في ال intestine؟ الأملاح، + +64 +00:04:58,220 --> 00:05:01,860 +فاكرهين؟ فاكرهين دي يعني إذا زاد الأملاح، لازم + +65 +00:05:01,860 --> 00:05:09,070 +النسم يخففها، ده نائزالمتعارف عليها في إدارة لكن + +66 +00:05:09,070 --> 00:05:12,450 +الأسباب بتكون متنوعة لماذا لما تم الامتصاص أو + +67 +00:05:12,450 --> 00:05:16,570 +لماذا تم التراكم بتكون أسباب مختلفة فهذه سببها ان + +68 +00:05:16,570 --> 00:05:20,910 +ال micro-ply اللي موجودة مادت كيف مادت؟ عن طريق + +69 +00:05:20,910 --> 00:05:25,390 +attachment بنوع خاص من ال ply بيسمونه bundle + +70 +00:05:25,390 --> 00:05:32,280 +forming bundle معناها حزمة bundle forming plyأو + +71 +00:05:32,280 --> 00:05:37,400 +البكتيريا بتعمل stimulation لل Intracellular + +72 +00:05:37,400 --> 00:05:42,460 +Calcium Level و rearrangement لل Intracellular + +73 +00:05:42,460 --> 00:05:42,900 +Actin + +74 +00:05:45,540 --> 00:05:48,060 +اللي أنا المرة الماضية قولتلكم عنها ال tide 3 + +75 +00:05:48,060 --> 00:05:51,080 +secretory system قولتلكم اتفرج عليها على اليوتيوب + +76 +00:05:51,080 --> 00:05:55,480 +ان البكتيريا بتعمل injection لبعض ال proteins ال + +77 +00:05:55,480 --> 00:06:00,300 +proteins هتعمل modulation او modification للأكتين + +78 +00:06:00,300 --> 00:06:04,880 +بحيث انها تصبح انها ايه انترال سيلولار فابو سيترسس + +79 +00:06:04,880 --> 00:06:08,620 +يعني تاخد البكتيريا فمعظم ال + +80 +00:06:16,260 --> 00:06:21,700 +أخر من البكتيري اسمه Etec + +81 +00:06:21,700 --> 00:06:28,080 +اختلف عنها الأولنية بحرف ال P معنى T تتذكر + +82 +00:06:28,080 --> 00:06:30,920 +الكوليرة؟ اذا كنت تتذكر الكوليرة، مدام الterm + +83 +00:06:30,920 --> 00:06:35,060 +اليوم، what is the mechanism of action للكوليرة؟ + +84 +00:06:35,060 --> 00:06:36,200 +كيف الامتحان؟ + +85 +00:06:40,050 --> 00:06:43,710 +اللي بتعمل ال .. برضه بتزيد في رجلات الأمناح برضه + +86 +00:06:43,710 --> 00:06:47,870 +.. كيف؟ و أنا .. أنا السؤال كيف؟ الادمين يتطلب الـ + +87 +00:06:47,870 --> 00:06:52,990 +A1، يتطلب عن الأديولات الـ A6، بتعمل ال activation + +88 +00:06:54,570 --> 00:06:58,850 +فبتحول ال .. بتحول ال advanced cyclase بيحول ال + +89 +00:06:58,850 --> 00:07:05,050 +ATP لسجن ال EMB لسجن ال EMB بيزود .. بيحيم ال إثاث + +90 +00:07:05,050 --> 00:07:08,610 +لل electrolytes حتي تسيط كميةها، حتي تخفيفها + +91 +00:07:08,610 --> 00:07:12,410 +الشثمة من ال water ده ال ice blockers، ده ال ice + +92 +00:07:12,410 --> 00:07:14,970 +blockers، هاي .. هاي بالفعل، هاي ال story نفس + +93 +00:07:14,970 --> 00:07:18,510 +القصة، عارفين ليش نفس القصة؟ لإن أنا هتكلم عن + +94 +00:07:18,510 --> 00:07:22,690 +تكسين، ال action تبعه، بنوصف واحد كبرالايت تكسين + +95 +00:07:23,410 --> 00:07:26,310 +الكوليراي تكسينه بتاعة الانتروتوكشينك إيشريشيا + +96 +00:07:26,310 --> 00:07:30,070 +كوليراي will produce دياليا similar to that of + +97 +00:07:30,070 --> 00:07:33,650 +Vibrio cholera نتكلم شوية عن Vibrio cholera لإنه + +98 +00:07:33,650 --> 00:07:36,090 +مهم جدا لإذا عرفنا المعلومات اللي علي Vibrio + +99 +00:07:36,090 --> 00:07:37,710 +cholera هي نفس المعلومات اللي علي الانتروتوكشينك + +100 +00:07:37,710 --> 00:07:43,010 +إيشريشيا كولرا الدياليا احنا قلنا جاب اللوحة ان + +101 +00:07:43,010 --> 00:07:47,490 +الستول is not as shared with blood مافي شي مش مدمم + +102 +00:07:57,110 --> 00:08:00,390 +أي دياريا في ال rice water لما بنغسل الرز أشبه كله + +103 +00:08:00,390 --> 00:08:05,430 +المياه ميليكي و whiteish صح ولا لأ؟ هناك + +104 +00:08:05,430 --> 00:08:09,050 +resemblance ما بين الدياريا و ما بين ال rice water + +105 +00:08:09,050 --> 00:08:13,610 +stool لذلك لما نتقف الناس في حالة الأوبع بنقولهم + +106 +00:08:13,610 --> 00:08:17,570 +أن أي دياريا في حالة الوباعة أي دياريا هي + +107 +00:08:17,570 --> 00:08:21,130 +suspected to be as a result of vibrio cholera + +108 +00:08:26,750 --> 00:08:33,830 +Lose stool per day لكن لما يكون في رايس water + +109 +00:08:33,830 --> 00:08:41,440 +stool هذا مدعاة لمش حقعالى الطبيب ان يعالج المريض + +110 +00:08:41,440 --> 00:08:46,600 +على انه كوليرة قبل ما يبدأ .. قبل ما يعني يكون فيه + +111 +00:08:46,600 --> 00:08:49,520 +تشخيص نهائي يبقى بيصير فيه ان انا اتقال ان هو + +112 +00:08:49,520 --> 00:08:52,660 +المريض اندو كوليرة ليه احنا مهتمين باننا نعالج + +113 +00:08:52,660 --> 00:08:57,300 +المريض بسرعة من جانب الجهد التشخيص لحاجتين لمصلحة + +114 +00:08:57,300 --> 00:09:00,640 +المريض نفسه ان احنا بدنا .. لان ال .. ال .. ال .. + +115 +00:09:00,640 --> 00:09:01,020 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +116 +00:09:01,020 --> 00:09:01,360 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +117 +00:09:01,360 --> 00:09:01,820 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +118 +00:09:01,820 --> 00:09:02,100 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +119 +00:09:02,100 --> 00:09:04,340 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +120 +00:09:04,340 --> 00:09:04,520 +.. ال + +121 +00:09:08,550 --> 00:09:13,590 +والمسألة التانية عشان خاطر المجتمع هذا الشخص + +122 +00:09:13,590 --> 00:09:18,650 +المصاب يشكل reservoir للبكتيريا فمن واجب انا ادور + +123 +00:09:18,650 --> 00:09:23,110 +عليهم و اعالجهم يعني حتى العائلة اللي بيكون فيها + +124 +00:09:23,110 --> 00:09:26,670 +فرد اصيب غالبا لازم اعملهم quarantine quarantine + +125 +00:09:26,670 --> 00:09:30,810 +يعني حجز حجب الصحي عشان انا اطلع مين منهم ممكن + +126 +00:09:30,810 --> 00:09:33,070 +يكون exposed او ممكن اعطيه + +127 +00:09:36,940 --> 00:09:41,720 +إذا احنا معنين لحظة rice water stool معنوعة صغيرة + +128 +00:09:41,720 --> 00:09:45,860 +طبعا استخدمناها في نشر الوعي في حالة اللي هو + +129 +00:09:45,860 --> 00:09:51,060 +الأوبيا مش بس الطبيب بيقصر او بتخلع ال protocol في + +130 +00:09:51,060 --> 00:09:53,680 +حالة الأوبيا احنا كمان بتخلع ال protocol في + +131 +00:09:53,680 --> 00:09:57,440 +المختبر كيف نتخلع ال protocol؟ هعطيكم مثل الكورية + +132 +00:09:57,440 --> 00:10:03,320 +نفسيقررنا عينة زرعناها بعد 24 ساعات تطلع لمستعملها + +133 +00:10:03,320 --> 00:10:08,360 +صح؟ بعد 24 ساعات نطلع عليها ساكروس فرمنتر من طول + +134 +00:10:08,360 --> 00:10:11,280 +مش عارف شوه نعملها biochemical test كمان 24 ساعات + +135 +00:10:11,280 --> 00:10:17,060 +صح؟ هجدت صار الآن 48 ساعات و 24 ساعات أخرى + +136 +00:10:17,060 --> 00:10:21,620 +لستيرولوجي زائد ال sensitivity testing هي 72 ساعات + +137 +00:10:21,620 --> 00:10:25,310 +كاملةهل من المنطق ان في حالة الأوبيا انتظر 48 + +138 +00:10:25,310 --> 00:10:29,370 +ساعة؟ طب انا اتقال كيف باكسل ال protocol بعد 24 + +139 +00:10:29,370 --> 00:10:32,510 +ساعة المستعمرة بتنمو Suspected Colonist ايه يعني + +140 +00:10:32,510 --> 00:10:35,110 +Suspected Colonist؟ مشترك بيها، يلها مواصفات + +141 +00:10:35,110 --> 00:10:38,210 +معينة، بدل من بعملها biochemical test تجارب 24 + +142 +00:10:38,210 --> 00:10:42,770 +ساعة، بعملها فحص serology واحد مباشر او فحصين one + +143 +00:10:42,770 --> 00:10:45,130 +minute test one minute test يعني ما بيستخدموا + +144 +00:10:45,130 --> 00:10:50,390 +معايا طويلة فباقتصر من ال diagnosis حوالي 48 ساعة + +145 +00:10:51,370 --> 00:10:54,790 +هذا لايعني ان انا لا اكمل إجراءات روتينية لكن انا + +146 +00:10:54,790 --> 00:10:58,470 +بهذا الحال بعمل تقريرين تقرير مبدئي وتقرير نهائي + +147 +00:10:58,470 --> 00:11:03,010 +حتى اوفر وقت لان احنا في حالة غير طبيعية اسمها + +148 +00:11:03,010 --> 00:11:08,130 +ability مكتر، لقيت فرازي ان هي ال electrolyte + +149 +00:11:08,130 --> 00:11:15,490 +مابنحلش الا بالناء يعني بالواتر يعني نتخلص من كمية + +150 +00:11:15,490 --> 00:11:20,290 +ال electrolyte بالناء صحيحيعني هينتج المشكلة + +151 +00:11:20,290 --> 00:11:24,590 +تانية؟ ما هو طبعا مشكلتين هينتج، حاسس ان هناك نقص + +152 +00:11:24,590 --> 00:11:28,610 +في المياه و نقص في الالكتروليات، لذلك اي قيمة + +153 +00:11:28,610 --> 00:11:34,640 +لبيارية should include two measureswater + +154 +00:11:34,640 --> 00:11:38,160 +replacement اللي هو الـrehydration وعالطريق المحلي + +155 +00:11:38,160 --> 00:11:42,260 +و ال electrolyte بتسمعوا بالمصريين .. أخوان + +156 +00:11:42,260 --> 00:11:46,040 +المصريين اللاطفين كان عندهم دعاية مشهورة لمعالجة + +157 +00:11:46,040 --> 00:11:52,700 +.. كان اسمه محلول معالجة الجفاف و جعلوا يمكن سنين + +158 +00:11:52,700 --> 00:11:59,540 +يعملوا عملية تسويق لهذا المنتج على التلفزيون لأنه + +159 +00:11:59,540 --> 00:12:01,660 +ديارية في العالم التالت + +160 +00:12:06,060 --> 00:12:08,880 +هو قاطر رقم واحد وبالتالي احنا مضطرين بالفعل + +161 +00:12:08,880 --> 00:12:13,320 +نعالجه بانه نرجعله المي اللي فقدها ونرجعله اللي هو + +162 +00:12:13,320 --> 00:12:17,100 +ال electrolyte اللي فقدها فجزء مهم جدا البعض + +163 +00:12:17,100 --> 00:12:21,100 +بيعتقد ان إيقاف الديارية هو أمر مهم لإيقاف + +164 +00:12:21,100 --> 00:12:25,540 +الديارية البعض بيظن انه غير مهم وبالعكس قد يؤذي + +165 +00:12:25,540 --> 00:12:31,490 +المرضلذلك لا تستخدم الانفيا النهار اسمها ايموديوم + +166 +00:12:31,490 --> 00:12:34,590 +معروف عن ان هو هذا بيوقف حركة الدودة .. الحركة + +167 +00:12:34,590 --> 00:12:37,790 +الدودية اللي امها هو هذا محدد ببتاعته خلال نص ساعة + +168 +00:12:37,790 --> 00:12:41,470 +وجه في الإسهال فبقى إذا أحنا متفقين إن الإسهال هو + +169 +00:12:41,470 --> 00:12:44,950 +reaction طبيعي من الدسم التخلص من ال micro + +170 +00:12:44,950 --> 00:12:48,690 +organism فبالتالي إذا وقفنا الإسهال بطريقة هذه + +171 +00:12:48,690 --> 00:12:53,380 +الميكروب والتوكسين هيبقى له موجودلكن الأفضل اللي + +172 +00:12:53,380 --> 00:12:57,240 +ما حكينا إن السوائل والإلكتروبات اللي بتفقدها + +173 +00:12:57,240 --> 00:13:01,660 +نعوضها والجانب التاني ان يعالج العامل المسبب نتخلص + +174 +00:13:01,660 --> 00:13:05,260 +منه إذا كان بالبروكلية إذا كان سالمونيلا إذا كان + +175 +00:13:05,260 --> 00:13:08,020 +إشريشة إذا كان شجلة إذا كان انتميبة أو whatever + +176 +00:13:08,020 --> 00:13:12,620 +causes it احنا خرجنا عن النص كتير لكن كله عشان + +177 +00:13:12,620 --> 00:13:13,620 +نوضع نقطة واحدة + +178 +00:13:21,760 --> 00:13:26,740 +و it causes conditions similar to cholera بسبب + +179 +00:13:26,740 --> 00:13:29,840 +الخط العزيزي هنا دياريا like cholera زي ما حكينا + +180 +00:13:29,840 --> 00:13:34,820 +milder أخف شوية من ال cholera لإن ال growth و ال + +181 +00:13:34,820 --> 00:13:38,060 +multiplication تبع الإجريشيا الcholera أقل درجة من + +182 +00:13:38,060 --> 00:13:42,280 +مين من ال vibrio cholera ممكن تتشر في الحضانات + +183 +00:13:42,280 --> 00:13:45,880 +يعني في الأطفال اللي عايشين في الحضانات أو أول + +184 +00:13:45,880 --> 00:13:48,000 +حاجة بيسموها travelers يعني ال travelers يعني يعني + +185 +00:13:48,000 --> 00:13:51,380 +المعروف عن إسهال المسافرينالذي ينتقل من مكان إلى + +186 +00:13:51,380 --> 00:13:56,020 +مكان وتتغذى عليه الطبيعة الجو والغذاء ممكن يصاب + +187 +00:13:56,020 --> 00:14:02,800 +بهذه الانفكار caused by LT ST أو both LT and ST LT + +188 +00:14:02,800 --> 00:14:06,760 +اللي هو ال bio-toxin وهو اللي يشبه الكودا توكسين + +189 +00:14:06,760 --> 00:14:13,120 +LT توكسين والST stable toxin يعني فكرة كلمة + +190 +00:14:13,120 --> 00:14:14,220 +الفايرو stable + +191 +00:14:17,150 --> 00:14:21,310 +إذا ال L-T و L-S-T توكسين اللي بيشتغله بالطريقة + +192 +00:14:21,310 --> 00:14:25,570 +التابعة ال heat-loving toxin like cholera gene او + +193 +00:14:25,570 --> 00:14:28,670 +cholera toxin it activates adenylate cyclase + +194 +00:14:28,670 --> 00:14:33,190 +enzyme بيرفع من ال cyclic AMP بيزيد من ال + +195 +00:14:33,190 --> 00:14:38,620 +secretions لل water ionsالـ hate stable toxin + +196 +00:14:38,620 --> 00:14:42,780 +بيشبه الأولان تماما في الميكانيزم بس بدل ما يشتغل + +197 +00:14:42,780 --> 00:14:46,620 +على قدرات Cyclase enzyme بيشتغل على جوانالات اللي + +198 +00:14:46,620 --> 00:14:49,800 +هو من الجوانين جوانالات Cyclase enzyme بيسيطروا + +199 +00:14:49,800 --> 00:14:55,040 +activation بيزيد كمية الـ cyclic GMP بدل ما يزيد + +200 +00:14:55,040 --> 00:14:58,260 +ال secretion ممنع ال absorption ماهي نفس المحصلة + +201 +00:14:58,260 --> 00:15:02,360 +ده إذا مانعنا الabsorption .. ال + +202 +00:15:04,570 --> 00:15:09,590 +أه فبيمنع ال .. ال .. ال ions من أنها تمتص و بتاع + +203 +00:15:09,590 --> 00:15:12,190 +اللي بيصير فيه كمية كبيرة من ال ions و الماء في + +204 +00:15:12,190 --> 00:15:18,850 +الداخل إذن ال heat level بيشبه لحد كبير ال heat + +205 +00:15:18,850 --> 00:15:22,550 +stable في النتيجة في المحاصلة لكن they work + +206 +00:15:22,550 --> 00:15:25,010 +different واحد بيشتغل على ال analyte واحد بيشتغل + +207 +00:15:25,010 --> 00:15:27,790 +على الgonalate هذا واحد واحد بيزيد ال secretion + +208 +00:15:27,790 --> 00:15:33,370 +واحد بيقلل من ال absorptionوهي واحدة اللى بيقدر او + +209 +00:15:33,370 --> 00:15:36,010 +بيزيد في الاخر بيصير فيه accumulation ل ال + +210 +00:15:36,010 --> 00:15:40,450 +electrolyte في الدسم هذه نفس المعلومات اللى بتوري + +211 +00:15:40,450 --> 00:15:43,310 +اللى هو ال toxin LT و ST كيف بيشتغلوا على اللى + +212 +00:15:43,310 --> 00:15:46,890 +اللى هو ال Y طبعا بتورينا كيف اشتركي بالله ال + +213 +00:15:46,890 --> 00:15:51,590 +toxin في امرأة microbe بتير مهم في الاشريشيا كلها + +214 +00:15:51,590 --> 00:15:53,250 +اسمه entero invasive + +215 +00:15:56,200 --> 00:16:00,200 +يعني حتى المصاميات هذه فيها على شوية خلافات، بمعنى + +216 +00:16:00,200 --> 00:16:03,480 +إنه قبل نجد إن واحد بيقول عنها intro-hemorrhagic، + +217 +00:16:03,480 --> 00:16:08,730 +وواحد بيقول عنها intro-invasiveفي مبرر لل .. لل .. + +218 +00:16:08,730 --> 00:16:14,650 +لل .. يعني لعدم الموضوع هذا ايش المبرر؟ انه بعض ال + +219 +00:16:14,650 --> 00:16:19,170 +strings بيمتلك صفتين او اكثر قد تشتركها مع بكتيري + +220 +00:16:19,170 --> 00:16:22,230 +اخرى و تنفصل عن بكتيري اخرى فبصير فيهم الاشتباه + +221 +00:16:22,230 --> 00:16:26,250 +فيها ده او خلينا نقول التباس في تسميع الميكروب لأ + +222 +00:16:26,250 --> 00:16:31,310 +احنا قدرنا نص اسمه بقول enteroinvasive Escherichia + +223 +00:16:31,310 --> 00:16:34,350 +coli هذا الميكروب عنده المفضل على انه يعمل + +224 +00:16:34,350 --> 00:16:35,930 +attachment لالميكروب + +225 +00:16:40,300 --> 00:16:43,600 +و البيولايت أقول عنها مكون أساسي في ال E.coli حتى + +226 +00:16:43,600 --> 00:16:47,160 +اللي بتسبب بيورنايترات infection لإن كيف بتعمل + +227 +00:16:47,160 --> 00:16:51,740 +بيوليفريتس عكس طيار صح؟ لازم يكون مباطلة هي على + +228 +00:16:51,740 --> 00:16:59,240 +الإلتصاق ال outer membranes بتبعت البكتيريا بتعمل + +229 +00:16:59,240 --> 00:17:05,300 +عملية modification في ال cell membrane تبع الخلالة + +230 +00:17:05,300 --> 00:17:08,920 +اللي هو ال cell و بتخلي البكتيريا تعملها + +231 +00:17:08,920 --> 00:17:13,150 +phagocytosisالان البكتيريا بالظبط البكتيريا ساعة + +232 +00:17:13,150 --> 00:17:17,410 +واحد موجودة الان جوا الخلايا ال intestinal cells + +233 +00:17:17,410 --> 00:17:22,950 +بتجي و تعمل destruction للخلايا عبر ال protein + +234 +00:17:22,950 --> 00:17:26,330 +synthesis inhibition فاكرين الشيجيلاتوكسين؟ + +235 +00:17:26,330 --> 00:17:31,210 +الشيجيلاتوكسين عشان بيشتغل بالضبط عبر ال ribosome + +236 +00:17:31,210 --> 00:17:35,130 +و بوقف ال protein synthesisفال S لاحظوش ايش اخر + +237 +00:17:35,130 --> 00:17:39,010 +جبنة بتقولها هنا؟ it resembles .. يعني المعنى اللي + +238 +00:17:39,010 --> 00:17:42,310 +حاكي الأولانية اللي جابلها كان resemble كلها و هاد + +239 +00:17:42,310 --> 00:17:45,450 +resemble شجلة و هاد الكلاب اللي بقى اكد عليه .. + +240 +00:17:45,450 --> 00:17:51,150 +اللي اكد عليه المحاضرة السابقةانه في transfer of + +241 +00:17:51,150 --> 00:17:55,530 +information من genus ال Vibrio للإشريشية من genus + +242 +00:17:55,530 --> 00:18:00,650 +الشجلة للإشريشية وبتالي في gene transfer او على + +243 +00:18:00,650 --> 00:18:04,210 +الأقل هذا مؤشر ان في gene transfer بين genus و + +244 +00:18:04,210 --> 00:18:06,470 +genus طيب + +245 +00:18:06,470 --> 00:18:13,770 +البكيلوجوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهوهو + +246 +00:18:14,900 --> 00:18:17,260 +يعني مافيش داعي انها تتكاثر و تطلع بعض الخلية و + +247 +00:18:17,260 --> 00:18:21,100 +تطلع خلية تانية، لأ من خلية إلى خلية، بنسميها + +248 +00:18:21,100 --> 00:18:26,820 +horizontal تمام؟ او lateral symptoms include + +249 +00:18:26,820 --> 00:18:31,000 +fever، هنفسر ال symptoms من وين اجت في غالب + +250 +00:18:31,000 --> 00:18:33,760 +الأحيان، fever، severe abdominal cramps اللي هي + +251 +00:18:33,760 --> 00:18:34,460 +مغض شديد + +252 +00:18:47,670 --> 00:18:52,690 +هو عبارة عن ميوكاس and pus and blood، ممكن يكون + +253 +00:18:52,690 --> 00:18:57,970 +كمان إيه؟ blood فقط، لكن لإن دولاريا frequent، + +254 +00:18:57,970 --> 00:19:03,070 +مرارة كراية، مافيش حاجة، اسمها stool الآن، فقط + +255 +00:19:03,070 --> 00:19:06,970 +الناتج هو الميوكاس والخلايا الميتة والبكتيريا + +256 +00:19:06,970 --> 00:19:11,670 +والصديد اللي بينتج، هنعرف من كل واحد منهم من وين + +257 +00:19:11,670 --> 00:19:17,820 +إيهit resembles gigalosis بتاعنا من وين اجت هاي ال + +258 +00:19:17,820 --> 00:19:22,520 +.. ال intestinal wall هاي ال microvilli ال + +259 +00:19:22,520 --> 00:19:26,760 +microvilli عليها ال mucus هاي البكتيريا عشان تلتصق + +260 +00:19:26,760 --> 00:19:31,420 +لازم تزيح لمucus و تلتصق بمين؟ بالبكتيريا بال .. + +261 +00:19:31,420 --> 00:19:34,200 +بال microvilli الآن مش بحاجة لحاجة إن ال mucus + +262 +00:19:34,200 --> 00:19:38,800 +انزعح إنه راح لأ موجود لكن في مكان قريب الآن + +263 +00:19:38,800 --> 00:19:42,000 +البكتيريا دخلت جواو موضت في الخلية الأولى و + +264 +00:19:42,000 --> 00:19:44,260 +التانية و التالتة و الرابعة و الخمسة أعلى تسطر من + +265 +00:19:44,260 --> 00:19:47,480 +الخلايا اللي موضتهم الخلايا بيختفوش عن وجه الأرض + +266 +00:19:47,480 --> 00:19:53,400 +فجأة لكن بيقولهم ميتين الاسم بيتخلص منهم و بيصيلهم + +267 +00:19:53,400 --> 00:19:57,240 +sloughing sloughing يعني ايه؟ يعني بيتقشر في + +268 +00:19:57,240 --> 00:20:01,040 +المعمل الآن بينزلوا و منزلوا في ال intestinal + +269 +00:20:01,040 --> 00:20:04,480 +lumen في اللي من في التجريب ايش عليهم الخلايا + +270 +00:20:04,480 --> 00:20:07,900 +دولة؟ هم lipidical cells ميتة فيهم بكتيريا و عليهم + +271 +00:20:07,900 --> 00:20:13,330 +ميوكاسيبقى الميوكاس اللى نزل فى الستول نادى عن + +272 +00:20:13,330 --> 00:20:17,630 +مين؟ عن موت الخلايا و سقوطها، يبقى بتوقع علاج فى + +273 +00:20:17,630 --> 00:20:21,390 +الستول ميوكاس ذات Epithelial cells ذات البكتيريا + +274 +00:20:21,390 --> 00:20:24,950 +نفسها ليها جرام نيجاتيف بصلاي أو باندنطلي، يعني + +275 +00:20:24,950 --> 00:20:25,970 +بشكل كبير + +276 +00:20:34,860 --> 00:20:37,400 +هى اللى بتاخد ال nutrient اللى ساعد على ال + +277 +00:20:37,400 --> 00:20:41,680 +absorption صح من ال micro villi بتاخدها و بتوزعها + +278 +00:20:41,680 --> 00:20:45,280 +على الرسم يبجى على طول ورا ال villi هيكون فيه + +279 +00:20:45,280 --> 00:20:49,380 +capillaries فهذه مصنوعة من PVC ولا plastic ولا ايش + +280 +00:20:49,380 --> 00:20:55,260 +مصنوعة مش برضه خلايا مش epithelial cells البكتيريا + +281 +00:20:55,260 --> 00:20:58,860 +بتتسخ فيها و بتعملها destruction يبجى ال blood + +282 +00:20:58,860 --> 00:21:01,700 +هيسير او ال capillaries هيسير لها rupture يعني + +283 +00:21:01,700 --> 00:21:06,930 +هتنفجر يبجى هينزلدم في الستولة يعني في عند ال + +284 +00:21:06,930 --> 00:21:10,870 +blood و في عند ال mucus طب إيش اللي جاب الصديد؟ + +285 +00:21:10,870 --> 00:21:16,630 +كلكوا بتعرفوا إن خليانة عندما تموت تصرخ تصرخ لتنبه + +286 +00:21:16,630 --> 00:21:19,470 +جهاز المنع الصراخ تبعها عبارة عن cytokines + +287 +00:21:19,470 --> 00:21:24,210 +messengers طبعا؟ ابتدي حضرة ال .. ال .. ال .. ال + +288 +00:21:24,210 --> 00:21:24,730 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +289 +00:21:24,730 --> 00:21:25,210 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +290 +00:21:25,210 --> 00:21:25,290 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +291 +00:21:25,290 --> 00:21:26,870 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +292 +00:21:26,870 --> 00:21:27,130 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +293 +00:21:27,130 --> 00:21:27,950 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +294 +00:21:27,950 --> 00:21:28,490 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +295 +00:21:28,490 --> 00:21:32,500 +ال .. ال ..الشجلة معروفة عنها خدوا بالكوا انها can + +296 +00:21:32,500 --> 00:21:36,740 +exist intracellular مش سهل نقتلها بمين؟ بال + +297 +00:21:36,740 --> 00:21:41,600 +phagocytes فكان مينية كبيرة من الخلايا اللي جاية + +298 +00:21:41,600 --> 00:21:48,420 +تحارب بتموت من مين؟ من الشجلة وبالتالي موت الخلايا + +299 +00:21:48,420 --> 00:21:52,660 +اللي هي ال phagocytes بينتج عنه صديد الصديد هو ال + +300 +00:21:52,660 --> 00:21:52,960 +bus + +301 +00:21:56,430 --> 00:22:00,170 +بتا هي عبارة عن ايه؟ عن white blood cell ماتة اللي + +302 +00:22:00,170 --> 00:22:05,550 +هي ال neutral field إذا انفسرنا نزول ال bus، نزول + +303 +00:22:05,550 --> 00:22:09,770 +الدم، نزول ال mucus، نزول ال epithelial cells، + +304 +00:22:09,770 --> 00:22:12,990 +نزول كمية كبيرة من اللي جراها، negative بس الله، + +305 +00:22:12,990 --> 00:22:16,730 +بالإضافة إلى الديارة، ما فسرناها خلاص الديارة، + +306 +00:22:16,730 --> 00:22:20,150 +مافيش epithelial cells تنفس، accumulation ديارة + +307 +00:22:21,290 --> 00:22:24,450 +بصرة الصراحية يبقى معنا بحكي أنه هذه البكتيريا + +308 +00:22:24,450 --> 00:22:28,710 +بتعمل شجلة like يبقى بنقدر نقول عنها dysentery + +309 +00:22:28,710 --> 00:22:33,570 +كلمة dysentery هي مميزاتها أنه فيها عندى stool، + +310 +00:22:33,570 --> 00:22:39,410 +دياريا، دياريا فيها blood، فيها mucus وفيها blood + +311 +00:22:39,410 --> 00:22:44,650 +و mucus بص و بالظبط بص و طبعا بالله فانا انا اقول + +312 +00:22:44,650 --> 00:22:50,760 +ان انا epithelial sexDysentery,Dysentery,Dysentery + +313 +00:22:50,760 --> 00:22:54,840 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +314 +00:22:54,840 --> 00:22:56,600 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +315 +00:22:56,600 --> 00:22:57,000 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +316 +00:22:57,000 --> 00:22:57,060 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +317 +00:22:57,060 --> 00:23:06,480 +,Dysentery + +318 +00:23:06,480 --> 00:23:06,560 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +319 +00:23:06,560 --> 00:23:10,560 +,Dysentery,Dysentery,Dysentery,Dysentery,Dysentery + +320 +00:23:10,560 --> 00:23:14,220 +,Dysentery + +321 +00:23:15,170 --> 00:23:18,330 +فى برايل يعني مافيش حرارة، مافيش سخونة عند المرض + +322 +00:23:18,330 --> 00:23:23,270 +يكون أحد أهم أخطر ال conditions اللى بتسببها هذه + +323 +00:23:23,270 --> 00:23:27,690 +البكتيريا هي ما يسمى بال HUS Hemolytic Uremic + +324 +00:23:27,690 --> 00:23:30,510 +Syndrome + +325 +00:23:32,270 --> 00:23:35,210 +وانا فجأة فانده مطورة لإنه انا باعتقادي ان هو + +326 +00:23:35,210 --> 00:23:38,550 +الميكروب يعني نوع ما مهمل في قطر غزة على الرغم من + +327 +00:23:38,550 --> 00:23:43,450 +انه الدراسات اللي أجريت محليا أثبتت وجود الميكروب + +328 +00:23:43,450 --> 00:23:47,690 +في مرضى و أثبتت وجود الميكروب في عينات غذائية منها + +329 +00:23:47,690 --> 00:23:53,000 +اللحم، الحوم، منها الدواجن، منها الخضرواتاللي تأكل + +330 +00:23:53,000 --> 00:23:57,960 +طازجة بمعناه انه ماتتعرضش لحرارة عالية فالبكتيريا + +331 +00:23:57,960 --> 00:24:02,700 +موجودة لكن الكشف عنها للأسف يعني عليها الاستفادة + +332 +00:24:02,700 --> 00:24:08,560 +مستشفياتنا ماتتجمش بالواقع في هذا الإطار لذلك هناك + +333 +00:24:08,560 --> 00:24:16,660 +بعض الأطفال قد نفقدهم بسبب فشل كله ناتج عن الإصابة + +334 +00:24:16,660 --> 00:24:23,130 +بهذا الميكروب بيُعطى مريض أنتبايتكفجأة اللي ما يخف + +335 +00:24:23,130 --> 00:24:28,330 +بتزيد الحالة عنده، ليش؟ لأن الخلايا لما تموت بتزيد + +336 +00:24:28,330 --> 00:24:31,930 +ال destruction اللي موجود طب احنا مش عارفين السبب + +337 +00:24:31,930 --> 00:24:34,610 +و قبلتها اللي بيجرب و انا انت بيتك واحد و اتنين و + +338 +00:24:34,610 --> 00:24:38,350 +تلاتة و اربع و للأسف ممكن يموت زي ما حكينا من + +339 +00:24:38,350 --> 00:24:43,410 +kidney failure و طفل kidney failure ناتجة عن + +340 +00:24:43,410 --> 00:24:46,270 +الاشريشيا كولا اللي سمينها in terror يمرجك اشريشيا + +341 +00:24:46,270 --> 00:24:51,090 +كولابتعرفوا الطعام التمييز بينها بتعمل hemolytic + +342 +00:24:51,090 --> 00:24:53,650 +anemia وبتعمل thrombocytopenia اللي اللي هو + +343 +00:24:53,650 --> 00:25:02,670 +platelet camp تسمعوا بالاسم هدا O157H7 + +344 +00:25:02,670 --> 00:25:07,570 +هي ال endo hemolytic E.coli هي نفسها ال E.coli + +345 +00:25:07,570 --> 00:25:12,910 +O157H7 اللي سمى في بريطانيا وامريكا hamburger + +346 +00:25:12,910 --> 00:25:14,510 +basselie او burger basselie + +347 +00:25:17,420 --> 00:25:22,940 +أول حالات تم اكتشافها للإيصال على الميكروب روبطت ب + +348 +00:25:22,940 --> 00:25:28,600 +under cooked hamburger under cooked يعني مش مطهي + +349 +00:25:28,600 --> 00:25:32,080 +جيدا و انا بقولكوا شغل في موضوع الطهي جيدا و مش + +350 +00:25:32,080 --> 00:25:37,820 +جيدا لأن عندهم هناك طبعا احنا .. اش .. اش العادة + +351 +00:25:37,820 --> 00:25:40,720 +الغذائية عندنا لما بنطلع رحلة مثلا بناخد نشوي على + +352 +00:25:40,720 --> 00:25:45,530 +البحر او في الخلاء مثلا او في منطقة و لا لا نشويهم + +353 +00:25:45,530 --> 00:25:48,770 +اكتر اشي بيعملوا بيعملوا اقراص همبرجر برضه + +354 +00:25:48,770 --> 00:25:54,050 +بيشوفوها فالان و جهزولهم ترمومترات قص الهمبرجر و + +355 +00:25:54,050 --> 00:25:57,550 +نحطوا فيه ترمومتر من جوا عشان نتأكل اذا اتحارست 75 + +356 +00:25:57,550 --> 00:26:01,870 +درجة او ايه عشان خاطر .. عشان خاطر نطمئن ان هذه + +357 +00:26:01,870 --> 00:26:07,330 +البكتيرا تكون ايه ماتة فبعند ال .. ال .. ال .. ال + +358 +00:26:07,330 --> 00:26:13,120 +ground beef او ال ground meat اللحم المفرومبيحتاجش + +359 +00:26:13,120 --> 00:26:16,180 +لحرارة عالية عشان ينتهي وانتوا بتعرفوا الفرق بين + +360 +00:26:16,180 --> 00:26:19,580 +انتوا معارف ال .. في الأمور هذه وابتعرفوا الفرق + +361 +00:26:19,580 --> 00:26:23,600 +بين طغي لحم مفروم وغير مفروم تمام؟ هذا ليه استغرف + +362 +00:26:23,600 --> 00:26:27,180 +وقت؟ وانا مستغرف وقت فبتعرف مادام مابستغرقش وقت دي + +363 +00:26:27,180 --> 00:26:32,200 +بالحرارة مابتصلش للمنطصف ب .. ب .. ب .. ب .. بدرجة + +364 +00:26:32,200 --> 00:26:35,020 +كتلة انها تكون قتلة ايه؟ الميكروباطام وبعدين بنقول + +365 +00:26:35,020 --> 00:26:39,240 +undercooked undercooked meat ممكن يكون سبب الأسباب + +366 +00:26:39,240 --> 00:26:45,560 +انتقال هذا ال micro orgasmفي كلمة شركة تانية اسمها + +367 +00:26:45,560 --> 00:26:52,120 +virotoxin و برضه هم عطيلة اسمها شركة like لإن عشان + +368 +00:26:52,120 --> 00:26:55,820 +انا اقولكوا في خلط ما بين ال entero invasive و ال + +369 +00:26:55,820 --> 00:27:00,210 +entero hemorrhageإشان الفيروتوكسين هنبدا أشرح و + +370 +00:27:00,210 --> 00:27:03,270 +هننهي في محالتنا عشان أعطيكوا فرصة كويسة أنه تلتقط + +371 +00:27:03,270 --> 00:27:06,710 +الأنفاس للاختبار ان شاء الله اليوم الفيروتوكسين + +372 +00:27:06,710 --> 00:27:10,810 +سمى الفيروتوكسين لإنه له تأثير على فيروس ال + +373 +00:27:10,810 --> 00:27:14,310 +culture إشان الفيروس ال culture نرجع لأفريقيا و + +374 +00:27:14,310 --> 00:27:18,970 +نروح على الغابات و نطلع على الشجرة و نلاقي قرد + +375 +00:27:18,970 --> 00:27:24,870 +أخضر اسمه African green monkey kidney أفضل African + +376 +00:27:24,870 --> 00:27:28,910 +green monkeyالـ African Green Monkey هدولة أخدنا + +377 +00:27:28,910 --> 00:27:32,230 +منهم kidney cells يعني أخدنا ال kidney تبعتهم و + +378 +00:27:32,230 --> 00:27:35,890 +أخدنا منها خلايا و عملنا منها cell culture cell + +379 +00:27:35,890 --> 00:27:41,190 +line cell line اسمه Vero cell line يبقى ال Vero من + +380 +00:27:41,190 --> 00:27:45,370 +عندي هي من اسم ال cell culture اللي أخدناها من ال + +381 +00:27:45,370 --> 00:27:49,370 +African Green Monkey تمام؟ أخدناها من عندي؟ من ال + +382 +00:27:49,370 --> 00:27:54,050 +kidneys يبقى أنا اللي عندي Vero cell cultureخونوا + +383 +00:27:54,050 --> 00:27:56,690 +بالكوا ان انتوا الانطباع اللى بتعرفوه على ال cell + +384 +00:27:56,690 --> 00:28:01,210 +culture انه بنستخدمها لدل مات مين؟ للمكتيريا؟ لأ + +385 +00:28:01,210 --> 00:28:06,330 +cell culture، للفيروسات، ال cell culture بننامي + +386 +00:28:06,330 --> 00:28:08,870 +فيها الفيروسات، احنا بننامي البكتيريا على + +387 +00:28:08,870 --> 00:28:11,810 +المقومكي، انا بسميها artificial media، مظبوط هذه + +388 +00:28:11,810 --> 00:28:13,890 +cell culture، هذه لمين؟ للفيروسات + +389 +00:28:17,450 --> 00:28:22,150 +من ضمن هذه الأغراض انه اشوف موارد سامة تأثير موارد + +390 +00:28:22,150 --> 00:28:26,150 +سامة على الخلايا طبعا الخلايا لما احنا بناخد من + +391 +00:28:26,150 --> 00:28:32,130 +كدني و بنزرعها في المعمل تطلعش كدني علما تطلع ما + +392 +00:28:32,130 --> 00:28:36,650 +يسمى ب mono layer فبقعة من الخلايا على السطح اللي + +393 +00:28:36,650 --> 00:28:41,150 +احنا زرعناها عليها mono layer فبقعة واحدة تحت + +394 +00:28:41,150 --> 00:28:46,060 +المايكروسكوب نقدر نشوف الخلايا بشكل واضحوكل نوع من + +395 +00:28:46,060 --> 00:28:51,640 +الخلايا إله شكله المميز تبعه وكل فيروس أو كل مركب + +396 +00:28:51,640 --> 00:28:54,820 +بيعمل destruction برضه ممكن يكون ال destruction + +397 +00:28:54,820 --> 00:29:01,760 +ميزة تميز الفيروس بمعنى بعض الفيروسات بتعمل + +398 +00:29:01,760 --> 00:29:07,320 +license للخلايا بتدمرهابعض الفيروسات بتجمع الخلايا + +399 +00:29:07,320 --> 00:29:10,680 +أربعة أو خمسة في خلية واحدة، بيسموها syncytia، + +400 +00:29:10,680 --> 00:29:14,380 +طبعا؟ فكل فيروس أو كل مُركّب له حاجة بيسموها + +401 +00:29:14,380 --> 00:29:19,020 +cytopathic effect، تأثيرات باطولوجية على الخلايا + +402 +00:29:19,860 --> 00:29:23,640 +لما جيبنا ال toxin تال إيشيشيكولاي اللي هي ال + +403 +00:29:23,640 --> 00:29:27,300 +enterohemorrhagic حطناها على الفيروس العمال + +404 +00:29:27,300 --> 00:29:31,880 +destruction، فما يسميناه فيرو توكسين، يعني سام + +405 +00:29:31,880 --> 00:29:35,340 +لغلايا الفيرو، عرفنا ايه الفيرو؟ عرفنا ايه؟ على + +406 +00:29:35,340 --> 00:29:42,770 +اخره، بتنتج ايضا hemolysinsغالبا بتصيب children + +407 +00:29:42,770 --> 00:29:46,430 +younger than 5 years old وبتسبب Hemorrhagic + +408 +00:29:46,430 --> 00:29:49,990 +Colitis Hemorrhagic Colitis يعني التهاب في القضاء + +409 +00:29:49,990 --> 00:29:54,490 +ونزف يكون فيه نزف نكمل ان شاء الله المرة القادمة + +410 +00:29:54,490 --> 00:29:56,810 +بعد اللي هو الامتحان + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..88dd55f20504b3f86433250232a1c404eafa46b1 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/j3TMld9XqFY_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 4999, "start": 20.59, "end": 49.99, "text": "بسم الله الرحمن الرحيم استكمل ان شاء الله موضوع الـ Gram Negative Bacilli الـ Enterobacteriaceae بدأنا بالمشهورة الاشريشيا كولاي تحدثنا عن الاشريشيا كولاي على إنها normal 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--> 00:00:36,190 +في مساق الأحياء الدقيقة الطبية الأساسية أو كما + +5 +00:00:36,190 --> 00:00:38,550 +تدعى باللغة الميزية essential medical microbiology + +6 +00:00:38,550 --> 00:00:43,530 +مش هندرس اليوم microbiology لكن هحنحاول قدر + +7 +00:00:43,530 --> 00:00:47,850 +الإمكان نسوق لهذا المساق نقولكوا أن هو هذا مساق + +8 +00:00:47,850 --> 00:00:53,120 +رائعجيد ممتاز أساسي وضروري لمهنة العلم الطبية + +9 +00:00:53,120 --> 00:00:56,760 +المخبرية يعني مش من المساقات الثانوية وانما من + +10 +00:00:56,760 --> 00:01:01,700 +المساقات الأساسية حتى نبدأ بالتسويق لهذا المساق + +11 +00:01:01,700 --> 00:01:04,540 +بدنا نبدأ من الكلمة الأخيرة microbiology + +12 +00:01:04,540 --> 00:01:07,640 +microbiology كلكم تعرفوا التعريف تبعها انه هي + +13 +00:01:07,640 --> 00:01:11,360 +دراسة الأحياء فكرة عام micro بتكلم عن دراسة + +14 +00:01:11,360 --> 00:01:15,140 +الأحياء الدقيقة يعني لو دينا نعرف تعريف بسيط بنقول + +15 +00:01:15,140 --> 00:01:19,380 +the study of microorganismال key word في هذا + +16 +00:01:19,380 --> 00:01:23,040 +الموضوع كلمة micro organism what is a micro + +17 +00:01:23,040 --> 00:01:27,720 +organism micro organism is a small living micro + +18 +00:01:27,720 --> 00:01:30,920 +organism that cannot be seen by the naked او اللي + +19 +00:01:30,920 --> 00:01:35,740 +بيسميها unaided eye يعني العين المجردة او العين + +20 +00:01:35,740 --> 00:01:39,520 +اللي مالهاش مساعدة مالهاش نضارات مالهاش microscope + +21 +00:01:39,520 --> 00:01:45,660 +لها اذا هذا هو ال micro biome لكن حتى التعريف هذا + +22 +00:01:45,660 --> 00:01:49,550 +اللي احنا عرفنا للأسفهنضطر نعمل justification أو + +23 +00:01:49,550 --> 00:01:56,610 +تبريرات لماذا ضمنا دودة طولها 7 متر ضمن الميكرو + +24 +00:01:56,610 --> 00:02:00,130 +بيولوجيا في علم البراسيتولوجيا ولماذا دودة + +25 +00:02:00,130 --> 00:02:04,030 +الاسكاريس اللي طولها 30 سانتي برضه مدرسة تحت + +26 +00:02:04,030 --> 00:02:06,890 +المايكرو هل .. هل بدنا مايكروسكوب حتى نشوف دودة 30 + +27 +00:02:06,890 --> 00:02:13,010 +سانتي مصطرة؟ بدناش مايكروسكوبone stage of the life + +28 +00:02:13,010 --> 00:02:16,190 +cycle of this parasite is microscopic يعني مرحل + +29 +00:02:16,190 --> 00:02:23,010 +مرحل حياتها بتكون مجهرية برضه مش مبرر احنا كبشر + +30 +00:02:23,010 --> 00:02:27,490 +مرحل من مرحل حياتنا بنكون مجهرين برضه صح ولا لأ + +31 +00:02:27,490 --> 00:02:34,630 +التبرير التاني ان they cause disease يعني بسببه + +32 +00:02:34,630 --> 00:02:37,850 +أمراض زي البكتيريا اللي هي بالفعل بينطبق عليها + +33 +00:02:37,850 --> 00:02:42,310 +التعريفبنعمل كمان justification الفيروسات نحن كنا + +34 +00:02:42,310 --> 00:02:46,990 +نتكلم عن living microorganisms هل الفيروسات living + +35 +00:02:46,990 --> 00:02:53,250 +microorganisms؟ هل هي أصلا ينطبق عليها organism؟ + +36 +00:02:53,250 --> 00:02:57,370 +هل هي خلوية؟ يعني إيه الخلية؟ الفيروسات؟ مالهاش + +37 +00:02:57,370 --> 00:03:01,950 +وبالتالي انطبق عليها جزء الأول من التعريف اللي هو + +38 +00:03:01,950 --> 00:03:06,470 +كلمة micro لأنها small لكن لم ينطبق عليها biology + +39 +00:03:06,910 --> 00:03:09,730 +وما ريح نجرسها في ال microbiology justification + +40 +00:03:09,730 --> 00:03:17,230 +تبرير ان الفيروسات تمتلك صفة من صفات الخلايا الحية + +41 +00:03:17,230 --> 00:03:23,430 +اللي و هي التكاثر عن طريق انها تستخدم host cell او + +42 +00:03:23,430 --> 00:03:28,350 +يعني خلايا عائل معين نبات او حيوان او حتى بعض + +43 +00:03:28,350 --> 00:03:32,110 +الفيروسات تتطفل على ال bacteria بيسموها + +44 +00:03:32,110 --> 00:03:35,550 +bacteriophytes الفطريات + +45 +00:03:36,710 --> 00:03:41,410 +كلكم أكلتم مشروم المشروم فطرية البعض منكم أصابوا + +46 +00:03:41,410 --> 00:03:45,190 +بين أصابع أرجليه التهابات فطرية اللي بتطلع رايحة + +47 +00:03:45,190 --> 00:03:49,470 +كريهة بعض الأحيان عند لبس الأحدى لفترات طويلة اللي + +48 +00:03:49,470 --> 00:03:54,290 +بيسموها قدم رياضية قدم رياضية ويعني بمان أخر في + +49 +00:03:54,290 --> 00:03:58,910 +microscopic وفي macroscopic لكن ال sports تبعتها + +50 +00:03:58,910 --> 00:04:03,130 +كلها microscopic سواء كانت الفطريات الكبيرة أو + +51 +00:04:03,130 --> 00:04:07,090 +الفطريات ال microscopic ال sports تبعتهاmicroscope + +52 +00:04:07,090 --> 00:04:11,630 +إذا متفقين على ال microbiology على تعريفها وعلى + +53 +00:04:11,630 --> 00:04:15,710 +التبريرات لماذا ضمنها بعض المخلوقات اللي هي لا + +54 +00:04:15,710 --> 00:04:20,370 +ينطبق عليها التعريف ضمن هذا التعريف نيجي لكلمة + +55 +00:04:20,370 --> 00:04:25,250 +medical ليش خصصنا medical شوفوا أنا في ال + +56 +00:04:25,250 --> 00:04:29,310 +microbiology أنا أعمل في مجال ال food microbiology + +57 +00:04:29,310 --> 00:04:35,130 +كلمة food food testing services يعني في عمليةفحص + +58 +00:04:35,130 --> 00:04:38,570 +الأغذية، فحص سلامة الأغذية في زمايا إيه اللي + +59 +00:04:38,570 --> 00:04:43,110 +بيشتغلوا في مجال تصنيع الأغذية، microbiology إيه + +60 +00:04:43,110 --> 00:04:45,970 +في مجال ال environmental microbiology، البيئة، + +61 +00:04:45,970 --> 00:04:48,470 +بروح على البحر باخد عينات و بعملها testing، هذا + +62 +00:04:48,470 --> 00:04:53,230 +بيئة ولا لأ، برد ناخد من الهوا عينات و بنتحصها، + +63 +00:04:53,230 --> 00:04:57,430 +هذا environmental microbiology قبل قدر السنوات + +64 +00:04:57,430 --> 00:05:01,670 +أنتجنا بكتيريا لمكافحة البعوض، كنا .. يعني نمّي + +65 +00:05:01,670 --> 00:05:05,160 +البكتيريا، بدنا البكتيريا نفسهاوبنرشها على + +66 +00:05:05,160 --> 00:05:10,660 +المستنقعات يرقات البعوض بتاكلها و بتموت هذا اسمه + +67 +00:05:10,660 --> 00:05:14,500 +Environmental و Industrial في نفس اللوجب ليش؟ احنا + +68 +00:05:14,500 --> 00:05:18,380 +قضينا على بعوض او مش قضينا بالاحرار خففنا لان كلمة + +69 +00:05:18,380 --> 00:05:22,720 +قضينا موضوع صعب خففنا من البعوض بهذا التقنية إذا + +70 +00:05:22,720 --> 00:05:26,240 +ال microbiology في مجالات متنوعة حمض الليمون اللي + +71 +00:05:26,240 --> 00:05:29,940 +بيستخدموه في المطبخ هذا تسعة و تسعين و تسعة من + +72 +00:05:29,940 --> 00:05:38,130 +عشرة من في الأسواق هو منتوج منالفطريات معظم + +73 +00:05:38,130 --> 00:05:42,290 +ال antibiotics اللى بتتناولوها فى العلاجات هى منتج + +74 +00:05:42,290 --> 00:05:47,270 +من منتجات ال micro organism يبقى مقدرش احصل ال + +75 +00:05:47,270 --> 00:05:51,470 +microbiology فى ال medical microbiology ولا بقدر + +76 +00:05:51,470 --> 00:05:55,770 +أسيب ال microbiology فاضى مفتوحة فاضى فاضى مظبوط + +77 +00:05:55,770 --> 00:05:59,510 +وبالتالي حددت انا في industrial اقولكوا فى ال food + +78 +00:05:59,510 --> 00:06:02,960 +microbiology صح فى انwater microbiology مع ان ال + +79 +00:06:02,960 --> 00:06:06,520 +water كان من مين ال food في ال food microbiology + +80 +00:06:06,520 --> 00:06:10,440 +صرنا نصنف الأغضية و نعطيها كاسم microbiology دلوقت + +81 +00:06:10,440 --> 00:06:15,780 +على توسع العلوم او علم الميكروبيري و تداخله في كل + +82 +00:06:15,780 --> 00:06:20,220 +المجارات هعطيكم مجارة رائعة جدا قبل سنتين دخلنا + +83 +00:06:20,220 --> 00:06:25,360 +تعرف البطون؟ لمين بيشتغلون بالبطون في الجامعة؟ + +84 +00:06:25,360 --> 00:06:28,360 +لندسة المدنية دلوقت هم اللي بيشتغلوا بالبطون احنا + +85 +00:06:28,360 --> 00:06:33,170 +مش دخلنا فيه بالبطونطيورة من قسم التحيات الطبية + +86 +00:06:33,170 --> 00:06:38,270 +لأن هي مؤيدة و بتدرس ماليستير في القردن عملنا + +87 +00:06:38,270 --> 00:06:43,690 +دراسة على استخدام البكتيريا في تقوية الإسمنت + +88 +00:06:43,690 --> 00:06:47,170 +المسلح يعني أضافنا بكتيريا من نوع معين و أضافنا + +89 +00:06:47,170 --> 00:06:52,330 +بعض ال chemicals نعمل reaction نخلي البطون يزداد + +90 +00:06:52,330 --> 00:06:55,510 +قدرته أو فيسموه compressive strength test قوة + +91 +00:06:55,510 --> 00:07:03,930 +تحمله إلى 30% إضافيوقللنا من قدرة امتصاصه الى اكثر + +92 +00:07:03,930 --> 00:07:09,890 +من 30% ايضا بمعنى اخر صار ان أسمنت مقاوم للرطوبة و + +93 +00:07:09,890 --> 00:07:14,670 +أسمنت أكتر صلابة و أكتر قدرة على التحمل كل هذا ضمن + +94 +00:07:14,670 --> 00:07:22,570 +من؟ لكن للأسف جدش الصورة اللى وصرتها جميلة السيئين + +95 +00:07:23,220 --> 00:07:27,920 +من الميكروبات هم اللي أكتر سمعة يعني هم الأكتر + +96 +00:07:27,920 --> 00:07:32,340 +شغرة في العالم وبالتالي لما بنقول ميكروبات بيخطروا + +97 +00:07:32,340 --> 00:07:36,220 +على بالك وليش جيد وليش سيء سيء لأنه ال medical + +98 +00:07:36,220 --> 00:07:42,560 +microbi الانسان يتذكر فقط من الميكروبات اللي سببله + +99 +00:07:42,560 --> 00:07:47,180 +أداء لكن تلك اللي تسبب أداء لا تشكل واحد إلى اتنين + +100 +00:07:47,180 --> 00:07:52,900 +في المية من مجموعة الميكروبات إذا من 98% إلى 99%من + +101 +00:07:52,900 --> 00:07:58,480 +الميكروبات ذو فائدة و ذو فائدة عظيمة تخيلوا .. + +102 +00:07:58,480 --> 00:08:05,420 +تخيلوا لو أن أشعة ما ضربت كوكب الأرض و قتلت كل + +103 +00:08:05,420 --> 00:08:09,220 +البكتيريا و المخلوقات الميكرو أورجينزية ما الذي + +104 +00:08:09,220 --> 00:08:12,920 +سيحدث .. عدا أني هبطل أدرس micro biology أنا بقى + +105 +00:08:12,920 --> 00:08:16,660 +مدمرت الميكروبات خلاص ما الذي سيحصل لكوكب الأرض + +106 +00:08:16,660 --> 00:08:24,420 +بعد عدة سنوات كل المخلوقات هتموتإذا بنقدر نقول إنه + +107 +00:08:24,420 --> 00:08:28,060 +هدف إحنا بنحن نتكلم عن ناحية نظرية ليش استنتجنا + +108 +00:08:28,060 --> 00:08:33,840 +هذا الاستنتاج؟ لأنه الطاقة الكامنة فينا احنا .. + +109 +00:08:33,840 --> 00:08:39,020 +احنا كانت طاقة كامنة خزّلناها بشكل غداء تقزّن بشكل + +110 +00:08:39,020 --> 00:08:42,260 +carbohydrate و protein ودهون في دسم الإنسان + +111 +00:08:42,260 --> 00:08:45,920 +بالإضافة إلى عناصر زي ال carbon و ال nitrogen و ال + +112 +00:08:45,920 --> 00:08:48,500 +phosphorus و ال oxygen كل هذه العناصر اللي مختزنة + +113 +00:08:48,500 --> 00:08:53,180 +في أجزامنا تخيلوا أنه هذه العناصرلم تدور في + +114 +00:08:53,180 --> 00:08:56,560 +الطبيعة لم تدور يعني ما انا اخر ماموتناش احنا + +115 +00:08:56,560 --> 00:08:59,460 +ماتحللناش مش اقصد احنا بس احنا على مستوانا + +116 +00:08:59,460 --> 00:09:03,420 +المخلوقات الأخرى جميعا النباتات طب مين اللي بيجوم + +117 +00:09:03,420 --> 00:09:07,320 +بعمل التحلل هذه ال micro organisms إذا ال micro + +118 +00:09:07,320 --> 00:09:12,320 +organisms بتجوم بدور رائع جدا في الطبيعة وبالتالي + +119 +00:09:12,320 --> 00:09:16,560 +لا يجب علينا أن ننسى كل هذا الدور الرائع و اللخص + +120 +00:09:16,560 --> 00:09:20,360 +حنا في مين في ال medical microbial ننتقل أقل مين + +121 +00:09:20,360 --> 00:09:25,560 +licensureessential يعني بيسموها أساسي لأ مش أساسي + +122 +00:09:25,560 --> 00:09:30,560 +ضروري فهل يعني .. هل معناها انه هن .. هن .. هنفصل + +123 +00:09:30,560 --> 00:09:34,460 +كثيرا في ال .. في المصاد هذا ولا هناخد المعلومات + +124 +00:09:34,460 --> 00:09:37,480 +الأساسية غالبا هناخد المعلومات الأساسية الطبية + +125 +00:09:37,480 --> 00:09:42,740 +بتوسع و ليس بعمق بمعنى هناخد معلومات كبيرة كثيرة + +126 +00:09:42,740 --> 00:09:49,640 +عفوا لكن مش عمق انا حاولت اقولكوا ان ال + +127 +00:09:49,640 --> 00:09:54,750 +microbiology microbiologyمرتبطة كثيرا بتعليف كلمة + +128 +00:09:54,750 --> 00:09:58,090 +مين micro organisms وحاولت أقولكوا إيش يعني + +129 +00:09:58,090 --> 00:10:02,030 +medical microbiology لكن بنركز عليها الآن بتركيز + +130 +00:10:02,030 --> 00:10:09,810 +أعلى ال medical microbes خلينا ننهج للموضوع احنا + +131 +00:10:09,810 --> 00:10:17,750 +لو يعني كنا نايمين وصحينا من النوم الساعة مثلا + +132 +00:10:17,750 --> 00:10:23,830 +تلاتة الفجرهنحط أصبعنا قدامنا خاصة في ضلقة الكهربا + +133 +00:10:23,830 --> 00:10:30,590 +مش هنشوف طاق خلينا صحيه لساعة عشر الصبح تمام؟ عشر + +134 +00:10:30,590 --> 00:10:34,870 +الصبح كيف يكون الجو؟ زي ما احنا الآن، نهار، بين + +135 +00:10:34,870 --> 00:10:39,090 +الأربعة، الأربعة الفجر إلى عشر الصبح، هل درجات + +136 +00:10:39,090 --> 00:10:45,420 +الظلمة و البنارة كانت واحدة؟ صرنا نشوف، لكنمش + +137 +00:10:45,420 --> 00:10:49,780 +بدرجة واضحة بعدين تدرجيا و بعدين سنشوف بدرجة واضحة + +138 +00:10:49,780 --> 00:10:54,460 +إذا ماكانش في عندي ظلام دانس أو نور ساطا بين + +139 +00:10:54,460 --> 00:10:57,640 +الفترين فقط لأ كان في عندي فترات مافيش فيها وضع + +140 +00:10:57,640 --> 00:11:02,960 +ترقية وهذا الكلام بدنا نحاول إن المفهوم هنا نغرصه + +141 +00:11:02,960 --> 00:11:08,800 +فيكوا لما نتكلم عن ال medical microbes ميكروب ممرض + +142 +00:11:08,800 --> 00:11:13,760 +و ميكروب غير ممرض نحط ذلك في الظلام ال dance + +143 +00:11:13,760 --> 00:11:18,940 +الممرضوالغير ممرض في النور الصافية لكن فيما بينهما + +144 +00:11:18,940 --> 00:11:23,900 +في ميكروبات قد يسبب أمراض وميكروبات أخرى أو نفس + +145 +00:11:23,900 --> 00:11:27,460 +الميكروب قد لا يسبب أمراض بمعنى لو رشينا عليكم إن + +146 +00:11:27,460 --> 00:11:31,660 +الميكروب هذا الآن جزء أمام البعض منكم ممكن يقول + +147 +00:11:31,660 --> 00:11:36,840 +البعض الأخر مانمرضش يجب ليش نفهم إن الممرض هو + +148 +00:11:36,840 --> 00:11:42,240 +absolute يعني مصطلح مطلق وإنما مصطلح relative في + +149 +00:11:42,240 --> 00:11:47,220 +عوامل أخرى تتحكم فيهفي دائما مايكروبات معروفة عنها + +150 +00:11:47,220 --> 00:11:51,880 +انها pathogenic و مايكروبات اخرى non pathogenic + +151 +00:11:51,880 --> 00:11:54,300 +pathogenic يعني ممرضة و non pathogenic يعني غير + +152 +00:11:54,300 --> 00:11:58,200 +ممرضة في ال medical microbiology غالبا هيتطرق + +153 +00:11:58,200 --> 00:12:02,720 +للنوعين، للممرضة و للغير ممرضة، لأنه بهمنا نعمل + +154 +00:12:02,720 --> 00:12:05,820 +differentiation، اللي هو عملية تمييز بين + +155 +00:12:08,580 --> 00:12:11,680 +أحد النقاط المهمة جدا في ال medical microbial اللي + +156 +00:12:11,680 --> 00:12:14,140 +دراستوها في التنمية العالمية و دراستوها في الأحياء + +157 +00:12:14,140 --> 00:12:18,080 +تبعت مستوى أول هو الفروقات بين ال prokaryotes و ال + +158 +00:12:18,080 --> 00:12:22,740 +eukaryotes طبعا هذكركم الفرق الرئيس عشان أنا بدي + +159 +00:12:22,740 --> 00:12:25,280 +أخد منه بس من جدول هذا بدي أخد منه معلومة أو + +160 +00:12:25,280 --> 00:12:29,860 +معلومتان عشان أوصلكم فكرة أساسية أن كيف بنستخدم أو + +161 +00:12:29,860 --> 00:12:33,240 +بنوظف الفروقات بين ال prokaryotes و ال eukaryotes + +162 +00:12:33,240 --> 00:12:38,620 +حقيقية النوة يعني في إيها غشاء نويةوبداية النوية + +163 +00:12:38,620 --> 00:12:43,800 +أنا مالهاش غشاء النوية هذا التعريف الأساسي لكن فيه + +164 +00:12:43,800 --> 00:12:47,380 +فروقات أخرى كثيرة شايفين الدول كبيرة أنا بدي أاخد + +165 +00:12:47,380 --> 00:12:52,460 +فرق أساسي أخر و أنطلق منه تلاف الريبوزوم ما هي + +166 +00:12:52,460 --> 00:12:57,420 +وظيفة الريبوزوم اسمها ال protein فاكرين الكورة + +167 +00:12:57,420 --> 00:13:00,660 +الكبيرة و الكورة الصغيرة اللي هي ال large subunit + +168 +00:13:00,660 --> 00:13:05,280 +و ال small subunit في الإيوكاريوت الريبوزوم هذا + +169 +00:13:05,280 --> 00:13:12,350 +القطعتينبنسميهم ATS AT يعني تمانين انا مش ارسم + +170 +00:13:12,350 --> 00:13:20,390 +تمانين اس نعم بالكود + +171 +00:13:20,390 --> 00:13:25,930 +سامحوني انا في الرسم يعني كتير قوي فهتنبهروا من + +172 +00:13:25,930 --> 00:13:30,770 +الرسمات اللي برسمها هاي أول دائرة اعتقد هذه أحسن + +173 +00:13:30,770 --> 00:13:31,670 +دائرة اللي برسمها انا + +174 +00:13:36,630 --> 00:13:42,910 +80S كل ريبوزون هذا لو حطيناه في جهاز ترسيب هيترصب + +175 +00:13:42,910 --> 00:13:49,550 +على سرعة 80 سرعة 80 لو فصلناهم عن بعض قطعة قطعة + +176 +00:13:49,550 --> 00:13:53,410 +هذا الكبيرة وهذا الصغيرة وحطينا الكبيرة في جهاز + +177 +00:13:53,410 --> 00:13:58,230 +الترسيب هيترصب على 50 از 2 وهذا هيترصب على 40 محدش + +178 +00:13:58,230 --> 00:14:01,830 +مولد يقول 60 40 100 لا مش جمال العملية العملية مش + +179 +00:14:01,830 --> 00:14:08,050 +جمال العملية سرعة ترسيبكله على بعضه ترسب عن تمانين + +180 +00:14:08,050 --> 00:14:12,890 +لما فصلناهم على ستين هذا على أربعين بينما الآخر + +181 +00:14:12,890 --> 00:14:15,930 +اللي هو اللي تبقى ال bacterial بروكاريوتز اسمه + +182 +00:14:15,930 --> 00:14:20,370 +سبعين أس يعني كله ترسب على كتير على سبعين الكبيرة + +183 +00:14:20,370 --> 00:14:24,110 +على خمسين و الصغيرة على تلاتين جمعناهم مع بعض أول + +184 +00:14:24,110 --> 00:14:27,630 +ما جمعناهم مش هذه المعلومة اللي هطلبها الوحيد اللي + +185 +00:14:27,630 --> 00:14:32,750 +هتحفظوها من المحاضرة هذه سبعين أس و تمانين أس + +186 +00:14:32,750 --> 00:14:37,780 +التمانين ستين اربعينوالخمسين تلاتين ثانية ليش أنا + +187 +00:14:37,780 --> 00:14:43,160 +بطلب المعلومة دلوقت أفهى لأنه إذا أحنا عرفنا + +188 +00:14:43,160 --> 00:14:49,020 +المعلومات وحفظناها في أدمغتنا ولم نستخدمها فهي + +189 +00:14:49,020 --> 00:14:53,520 +عديمة نفاية لا علم إلا ما ينفع لازم العلم يكون + +190 +00:14:53,520 --> 00:14:59,400 +نافع لازم نستفيد منه بناخد antibiotic لما بصيبنا + +191 +00:14:59,400 --> 00:15:03,890 +التهاب احنا ال antibioticبيقضي على ال protein + +192 +00:15:03,890 --> 00:15:06,550 +synthesis أو بيوقف ال protein synthesis في الخلايا + +193 +00:15:06,550 --> 00:15:12,110 +لماذا يوقف ال protein synthesis في البكتيريا ولا + +194 +00:15:12,110 --> 00:15:17,610 +يوقف ال protein synthesis في خلاينا لأنه بيستهدف + +195 +00:15:17,610 --> 00:15:23,590 +ال 30S ribosomal subunit ال 30S في من موجود هذه؟ + +196 +00:15:23,590 --> 00:15:27,510 +في البكتيريا ال بروكاريوتز طب هل فينا احنا احنا + +197 +00:15:27,510 --> 00:15:31,990 +ايه بروكاريوتز؟ هل فينا 30S؟ مش اللي احنا احنا 40S + +198 +00:15:33,350 --> 00:15:36,330 +استهدفنا .. استهدفنا او ال .. ال .. ال antibiotic + +199 +00:15:36,330 --> 00:15:41,350 +استهدف ال 30S ما استهدفش مين؟ طب ليش بيستهدف ال + +200 +00:15:41,350 --> 00:15:43,990 +30S بيستهدفش ال 40S؟ زي ما انتوا جيتوا على الغرفة + +201 +00:15:43,990 --> 00:15:48,250 +هذه يا بنات خاص نص ماجيتوش لغرف تانية للمحاضرة هذه + +202 +00:15:48,250 --> 00:15:52,810 +لانكو معاك ورقة ومسمحوا لكم الدخول في هذا الوقت + +203 +00:15:52,810 --> 00:15:57,250 +صح؟ ال binding بين المركبات وبين الخلايا بيكون + +204 +00:15:57,250 --> 00:16:00,650 +specificفبيكون فيه receptor لل antibiotic على ال + +205 +00:16:00,650 --> 00:16:05,590 +30S ومافيش receptor على ال 40S إذا هي استفادتنا من + +206 +00:16:05,590 --> 00:16:08,690 +معلومة اسمها فروقات ما بين البروكاريوت + +207 +00:16:08,690 --> 00:16:13,850 +والإيوكاريوت فارق آخر إحنا خلايانا كإيوكاريوت + +208 +00:16:13,850 --> 00:16:17,050 +مالناش cell wall صح؟ ولا نا cell wall؟ لو إلنا + +209 +00:16:17,050 --> 00:16:21,070 +cell wall كنا سنة زي الطاولة، خشب، مظبوط؟ بس + +210 +00:16:21,070 --> 00:16:23,590 +مالناش cell wall البكتيريا إلها cell wall + +211 +00:16:23,590 --> 00:16:29,210 +البنسالين المشهور عالميابيقتل البكتيريا بإنه + +212 +00:16:29,210 --> 00:16:34,010 +بيستهدف ال cell wall synthesis بيوقف تصنيع ال cell + +213 +00:16:34,010 --> 00:16:37,470 +wall يبقى بيقتل البكتيريا بيقتلناش احنا لإن احنا + +214 +00:16:37,470 --> 00:16:41,110 +أصلا ماناش cell wall إذا المعرفة دي و الفروق بين + +215 +00:16:41,110 --> 00:16:44,890 +ال eukaryote و ال prokaryote مهمة جدا لتطبيقات + +216 +00:16:44,890 --> 00:16:50,710 +مستقبلية كمان فرق تالت و مهم جدا اللي هو ال + +217 +00:16:50,710 --> 00:16:56,030 +chromosome أكام كرموزوم عند البشريعني 23 ضرب اتنين + +218 +00:16:56,030 --> 00:17:01,850 +اظن ان هما ازواج لكن في البكتيريا it is only one + +219 +00:17:01,850 --> 00:17:06,790 +single chromosome و هذا ال chromosome بيقول + +220 +00:17:06,790 --> 00:17:12,330 +circular يعني مالهوش طرف بينما كرموزوماتنا احنا + +221 +00:17:12,330 --> 00:17:17,410 +linear خطية زي الجلم الجلم له كام طرف؟ طرفين بس + +222 +00:17:17,410 --> 00:17:22,610 +الدائرة مالهاش ولا طرف ايش ميزة اتنين و واحد؟ هاي + +223 +00:17:22,610 --> 00:17:28,610 +كرموزوموهي المقابل إله على الجهة التانية نفترض إنه + +224 +00:17:28,610 --> 00:17:33,670 +في جين نفس الجين هيكون وين؟ في الجهة التانية هذا + +225 +00:17:33,670 --> 00:17:38,450 +الجين صار فيه خلل طفرة mutation الجين التاني ال + +226 +00:17:38,450 --> 00:17:43,610 +expression تبعه ما زال قادر على إنتاج نفس المنتج + +227 +00:17:43,610 --> 00:17:47,910 +وبالتالي لن نشعر إنه أصلا صار فيه خلل في هذا الجين + +228 +00:17:47,910 --> 00:17:52,010 +لإنه فيه جين بديل بينتج نفس الإنتاج يبقى الطفرة في + +229 +00:17:52,010 --> 00:17:56,820 +هذه الحالة لم تظهر نتائجهالكن بالبكتيريا it is + +230 +00:17:56,820 --> 00:18:02,240 +only one single chromosome وبالتالي أي تثرة هتظهر + +231 +00:18:02,240 --> 00:18:07,260 +و تثورة هتكتر نقطة مهمة it is easy to make + +232 +00:18:07,260 --> 00:18:12,200 +mutations in bacteria و بعض الأحيان احنا بنلجأ + +233 +00:18:12,200 --> 00:18:17,540 +لعمل mutation في البكتيريا لتحسين إنتاج منتج معين + +234 +00:18:17,540 --> 00:18:23,740 +مثلافي دراستنا أيام ال الستير كان فينا كطلاب نحاول + +235 +00:18:23,740 --> 00:18:27,560 +ننتج إنزين اسمه Alpha Amylase و الله عزلنا بكتيريا + +236 +00:18:27,560 --> 00:18:33,200 +و تمام اسمها Bacillus و ورايناها الدكتورة اللي + +237 +00:18:33,200 --> 00:18:37,700 +بتشرف علينا جالت كويسة بس إنتاجها قليل بدنا نحسن + +238 +00:18:37,700 --> 00:18:41,280 +إنتاجها طب كيف نحسن إنتاجها؟ جاءت يعملولها طفرات، + +239 +00:18:41,280 --> 00:18:46,420 +يعملولها طفرات انتوا يعني خربوها فاخدنا البكتيريا + +240 +00:18:46,420 --> 00:18:50,990 +و عرضناها لل ultra violet lightال ultraviolet + +241 +00:18:50,990 --> 00:18:54,890 +light تعمل mutations لفترات معينة زمانية trial and + +242 +00:18:54,890 --> 00:18:59,810 +error و في الآخر حصلنا على سلالة بتنتج alpha + +243 +00:18:59,810 --> 00:19:03,850 +amylase بكميات ضخمة بدون حساب طبعا الطفرة بالنسبة + +244 +00:19:03,850 --> 00:19:07,630 +لإن احنا نبلي إشلان كويسة إن احنا كبشر من وجهة + +245 +00:19:07,630 --> 00:19:11,190 +نظرنا احنا من وجهة نظر البكتيريا سيئة لأنها فقدت + +246 +00:19:11,190 --> 00:19:15,610 +السيطرة على إنتاج ال alpha amylase هذا سيء للطاقة + +247 +00:19:15,610 --> 00:19:20,830 +بالنسبة لها لكن في الآخرit is easy to make + +248 +00:19:20,830 --> 00:19:25,050 +mutations in bacteria لكن عكس الإنسان يقول إنه .. + +249 +00:19:25,050 --> 00:19:27,310 +إنه .. لإنه الـ diploid بيه عنده زوج من + +250 +00:19:27,310 --> 00:19:39,970 +البروموزومات وبالتالي بيكون الوضع أسلوب طيب + +251 +00:19:39,970 --> 00:19:43,230 +أنتوا بتعرفوا أنه الممالك تبع المخلوقات في التصنيف + +252 +00:19:43,230 --> 00:19:47,090 +احنا بحب التصنيف البشري و أعتقد أنه ت .. تروح + +253 +00:19:47,090 --> 00:19:50,950 +المكتب بعض الأحيان .. بعض الأحيانبنروح المكتبات، + +254 +00:19:50,950 --> 00:19:55,230 +بنلاقيش كوم كتب في المكتبات، بنلاقي أرفف وطوابق، + +255 +00:19:55,230 --> 00:19:57,990 +هذا طابق للمراجع باللغة الإنجليزية، للعلمية، + +256 +00:19:57,990 --> 00:20:01,910 +للطبية، للكيميا، للأحياء، مظلومة لتصنيفات تخيل لو + +257 +00:20:01,910 --> 00:20:07,070 +أنا بدي كتاب في الكيميا، و بروح لاجي كل كتب المكتب + +258 +00:20:07,070 --> 00:20:10,710 +كومه معاهم، يمكن ما حد ماميديا يطلع الكتاب، ويمكن + +259 +00:20:10,710 --> 00:20:15,110 +يطلع أخر كتاب، صح ولا لأ؟ فنحن نميل إلى التصنيف + +260 +00:20:15,110 --> 00:20:20,450 +والترتيب بغلط سهولة استرجاع المعلومةاسمها + +261 +00:20:20,450 --> 00:20:25,250 +retrieval system استرجاع المعلومات التصديق مر في + +262 +00:20:25,250 --> 00:20:30,590 +مراحل كتيرة على المخلوقات وفيها لحد الآن عليها + +263 +00:20:30,590 --> 00:20:36,110 +خلافات و الخلافات هادي هادي عن تشابك المعلومات و + +264 +00:20:36,110 --> 00:20:41,410 +التغيير بتم لأن معلوماتنا بتزيد يعني بعض الأحيان + +265 +00:20:41,410 --> 00:20:45,710 +مابنكتشفش مايكروفات جديدة ولا مخلوقات جديدة لكن + +266 +00:20:45,710 --> 00:20:48,720 +معلوماتنا بتزيد ليش معلوماتنا بتزيد؟لأنه في + +267 +00:20:48,720 --> 00:20:52,940 +research في أبحاث و لأنه في تقنيات كنا زمان ب + +268 +00:20:52,940 --> 00:20:55,460 +microscope عادي بعدين صرنا ب electron microscope + +269 +00:20:55,460 --> 00:20:59,940 +بعدين صرنا ب confocal microscopy صرنا بتقنيات + +270 +00:20:59,940 --> 00:21:05,520 +متطورة جدا وبتالي زادت معلومات فالتصنيف ليس يعني + +271 +00:21:05,520 --> 00:21:09,040 +شيء fixed ممكن يتغير و هنشوف هذا الكلام في + +272 +00:21:09,040 --> 00:21:12,200 +البكتيريا بعض الأحيان لكن الأنيمال أمامنا ماتغيروا + +273 +00:21:12,200 --> 00:21:14,340 +دلوقتي متانية أمامنا ماتغيروا animal and plant + +274 +00:21:14,340 --> 00:21:19,180 +ميزوا بين الحيوانات و بينالنباتات منذ زمن بعيد + +275 +00:21:19,180 --> 00:21:23,860 +البروتست إغلاق تشمل لبروتزوفة ومن الأشياء القريبة + +276 +00:21:23,860 --> 00:21:28,180 +الفنجاي اللي هي الفطريات مملكة لحالها البروكاريوت + +277 +00:21:28,180 --> 00:21:31,560 +عملوها مملكة وعملوا فيروسات مملكة كم مملكة صاروا + +278 +00:21:31,560 --> 00:21:38,320 +هذولا؟ ستة واحد اتنين دلتر اربع خمسة واحد ستة لكن + +279 +00:21:38,320 --> 00:21:42,940 +في شيء أعلى من المملكة أعلى من المملكة تسموه + +280 +00:21:42,940 --> 00:21:45,990 +domainمافي عندي domain eukaryote و eukaryote يعني + +281 +00:21:45,990 --> 00:21:47,970 +الـ eukaryotes لحظوا مين تحت الـ eukaryotes + +282 +00:21:47,970 --> 00:21:54,930 +animal, plant, protist و fungi تحت ال .. البكتيريا + +283 +00:21:54,930 --> 00:22:01,590 +في دمية البكتيريا أو الاركيا الوكاريوتس فقط مملكة + +284 +00:22:01,590 --> 00:22:07,530 +واحدة و ال viruses ليست .. ليست ضمن تصنيف ال + +285 +00:22:07,530 --> 00:22:11,510 +domains مملكة مالهاش domain، مملكة مالهاش domain + +286 +00:22:12,670 --> 00:22:17,070 +لكن اللي بتصله ان كل هذه المخلوقات اللى كوكب الأرض + +287 +00:22:17,070 --> 00:22:20,410 +تصنف علينها Eukaryotes وده ال mainly فقط ال + +288 +00:22:20,410 --> 00:22:23,130 +bacteria و ال archaea أول مرة يمول معنا مصطلح + +289 +00:22:23,130 --> 00:22:29,870 +archaea بنحاول نوضر مين بيعرف إيش معنى عالم آثار؟ + +290 +00:22:29,870 --> 00:22:37,730 +لغة لإنجليزية عالم آثار archaeologist archaeology + +291 +00:22:37,730 --> 00:22:42,770 +يعني علم الآثار و الآثار غالبا بتكون مستقبليةقديمة + +292 +00:22:42,770 --> 00:22:47,830 +.. قديمة فسمى للبكتيريا Archaia لأن العلماء بتوع + +293 +00:22:47,830 --> 00:22:53,030 +التصنيف و بتوع التطور تمام بيقولوا انها هي الأقدم + +294 +00:22:53,030 --> 00:22:56,230 +في السلسلة احنا طبعا كمسلمين و انتوا عارفين ان .. + +295 +00:22:56,230 --> 00:23:00,670 +ان .. ان .. معتقد ان الله سبحانه وتعالى هو خلق كل + +296 +00:23:00,670 --> 00:23:06,210 +هذه المخلوقات مش خلق بالتطور من الى .. من الى ال + +297 +00:23:06,210 --> 00:23:11,090 +Archaia قلنا انه في التصنيف بيسموها البكتيريا + +298 +00:23:11,090 --> 00:23:16,220 +القديمةلكن بتعطيكوا بعض الأمثلة على الاركيا وعلى + +299 +00:23:16,220 --> 00:23:21,080 +روعتها في هذا العالم بتعرفوا البحر الميت؟ لسه مو + +300 +00:23:21,080 --> 00:23:27,880 +بحر ميت مافيش فيه حياة صح؟ اليوم بتقولوا أن البحر + +301 +00:23:27,880 --> 00:23:32,680 +الميت ظلم وسمي بحر ميت على .. على .. لدفس السيطرة + +302 +00:23:32,680 --> 00:23:36,660 +هو ده لسه ميت لأنه بتحيا فيه بكتيريا من الاركيا + +303 +00:23:36,660 --> 00:23:43,140 +اسمها halobacterium halopium وكلمة halo ملحهاله + +304 +00:23:43,140 --> 00:23:47,720 +معناها ملح فهي محبة للملح وبتعيش في البحر المقلور + +305 +00:23:47,720 --> 00:23:52,040 +بناها عندنا وحاطناها في البحر تبعنا بتموت بدها ملح + +306 +00:23:52,040 --> 00:23:58,820 +تمام؟ بدها كمية كبيرة من الملح لو تعرفوا في يانبيه + +307 +00:23:58,820 --> 00:24:02,660 +مياه سخنة ساخنة قد تصل درجة الحرارة فيها إلى تسعين + +308 +00:24:02,660 --> 00:24:07,000 +إلى خمس و تسعين درجة مقوية هناك تعيش بكتيريا من + +309 +00:24:07,000 --> 00:24:15,360 +نوع اركيا هناك كهوف كهوف في جبالبيرشح منها acid + +310 +00:24:15,360 --> 00:24:21,080 +مادة حمضية sulfuric acid H2SO4 لو صابت جلدنا + +311 +00:24:21,080 --> 00:24:27,320 +هتحرقه هنا تعيش بكتيريا تنتج هذا الحمض من نوع مين؟ + +312 +00:24:27,320 --> 00:24:32,140 +من نوع Archa لذلك بعض العلماء بيطلقوا عليها + +313 +00:24:32,140 --> 00:24:39,080 +extremophileيعني البكتيريا المحبة للظروف المتطرفة، + +314 +00:24:39,080 --> 00:24:42,040 +البحر الميت بلوحة عالية، الحرارة العالية جدا اللي + +315 +00:24:42,040 --> 00:24:45,520 +بيقولون عنها في ال springs، الحمودة المرتفعة اللي + +316 +00:24:45,520 --> 00:24:49,360 +اتكلمنا عنها، إذا ال .. ال .. ال .. ال Archa هي + +317 +00:24:49,360 --> 00:24:53,560 +نوع من البكتيريا لكن غالبا بيعيش هنا في ظروف .. + +318 +00:24:53,560 --> 00:24:57,900 +فبتالي مش .. مش هلاجي Archa ضمن اللي هو ال medical + +319 +00:24:57,900 --> 00:25:00,920 +microbiome، مش هلاجي Archa ضمن ال medical + +320 +00:25:00,920 --> 00:25:03,760 +microbiome، هلاجي مين؟ فقط بكتيريا + +321 +00:25:07,860 --> 00:25:13,540 +أين نتوقع أن نجد micro organism؟ أكيد لكم أحد + +322 +00:25:13,540 --> 00:25:17,560 +موبايلز أه؟ فصل الماضي عملنا دراسة على .. + +323 +00:25:17,560 --> 00:25:20,920 +زميلاتكوا في القسم عملوا دراسة على ال mobile phone + +324 +00:25:20,920 --> 00:25:26,360 +و أظهروا نتائج يعني سيئة للغاية طبعا الإينات كانوا + +325 +00:25:26,360 --> 00:25:31,640 +أفضل من الذكور في المحافظة على نظافة ال mobile لكن + +326 +00:25:31,640 --> 00:25:35,270 +بشكل عام لجينا نسبةتتجاوز ال 50% من موبايلات + +327 +00:25:35,270 --> 00:25:41,330 +الطلبات تحتوي على potential pathogens يعني ممرضات + +328 +00:25:41,330 --> 00:25:48,930 +محتملة طيب بلاش نسأل السؤال بطريقة تانية عطوني + +329 +00:25:48,930 --> 00:25:56,410 +مكان بتعرفوا عنه أنتوا محقق الإجابة + +330 +00:25:56,410 --> 00:25:56,870 +بعيدة + +331 +00:26:00,400 --> 00:26:06,000 +أبطل عمليات هل تعقيم طبيعي و لا تعقيم صناعي تعقيم + +332 +00:26:06,000 --> 00:26:09,640 +الصناعي فإن حالة تعقيم طبيعي حالة معقمة يعني مثلا + +333 +00:26:09,640 --> 00:26:18,120 +فاكهة أكلتوها فكهة معقمة ال exterior تبع الأشياء + +334 +00:26:18,120 --> 00:26:21,560 +لازم يكون عليها micro wave يعني لو أخدنا منكم + +335 +00:26:21,560 --> 00:26:25,160 +مسحات من إيدكم و أنتم مغسلين و جايين لسه علاجة + +336 +00:26:25,160 --> 00:26:30,300 +micro wave تفاحة من برةمش فيها microorganism؟ فيها + +337 +00:26:30,300 --> 00:26:34,180 +microorganism أفضل من جوا مافيها اشي احنا أجسامنا + +338 +00:26:34,180 --> 00:26:39,620 +الجلد تبعنا الأنف الفم مليء بال microorganism ال + +339 +00:26:39,620 --> 00:26:44,400 +intestinal tract مليئة بال microorganism لكن الدم + +340 +00:26:44,400 --> 00:26:51,520 +تبعنا ال CSF يبقى في جسمنا احنا بنقدر نجسمه لجسمين + +341 +00:26:51,520 --> 00:26:56,960 +وهذا على فكرة أسهل تقسيمة في كل العلوم اللي + +342 +00:26:56,960 --> 00:27:00,140 +بتدرسوها في حياتكوافي الإنسان بنقسمولا جسمين + +343 +00:27:00,140 --> 00:27:04,940 +sterile و non-sterile parts أعضاء أو أجزاء معقمة + +344 +00:27:04,940 --> 00:27:07,420 +طبعا هذا تقسيم مهم لدني ليش لأن لو أنا صار عن + +345 +00:27:07,420 --> 00:27:11,160 +التهاب في الأنف و بدي أخد مسحة من الأنف عشان أشوف + +346 +00:27:11,160 --> 00:27:15,500 +المسبب للاتهاب الميكروب اللي طلع معايا الآن هل هو + +347 +00:27:15,500 --> 00:27:20,700 +من هو المسبب للاتهاب ولا من الموجودات الطبيعية + +348 +00:27:20,700 --> 00:27:23,600 +اللي بنسميها normal flora أو normal microbiota مش + +349 +00:27:23,600 --> 00:27:28,530 +عارفين مابنعرفش صح؟ هذه صعوبةفبنلاقي طرق للتفريق + +350 +00:27:28,530 --> 00:27:33,870 +بين الاتنين أمامنا ال microbes are everywhere و + +351 +00:27:33,870 --> 00:27:38,670 +اللي جالت الأخت اللي جالت أنه غرفة العمليات بنصرف + +352 +00:27:38,670 --> 00:27:42,490 +مسار كتير و فلوس كتير عشان نوفر ظروف معقمة في غرفة + +353 +00:27:42,490 --> 00:27:48,170 +العمليات يعني يكلفنا الأمر فلوس حتى نتخلص من ال + +354 +00:27:48,170 --> 00:27:53,130 +microorganism فالميكروبات دائما موجودةوما انها + +355 +00:27:53,130 --> 00:27:56,550 +موجودة في كل مكان ال air و ال soil و ال water و ال + +356 +00:27:56,550 --> 00:28:00,430 +animals و ال human body بنلاقي و احنا بنشتغل + +357 +00:28:00,430 --> 00:28:05,450 +microbiology فى المعامل بنلاقي طريقة مانلوثش + +358 +00:28:05,450 --> 00:28:12,430 +العينات مننا احنا مانتكلمش اذا انا اكون مضاف + +359 +00:28:12,430 --> 00:28:17,110 +مطهرين تمام نشتغل جنب اللهب مانتخلش الهوى اللى + +360 +00:28:17,110 --> 00:28:24,180 +موجود ينزل مانتكلمش مانتنفسشفي الطبخ تبعنا تبعا كل + +361 +00:28:24,180 --> 00:28:28,320 +هذه أشياء ممارسات يجب أن نتعلمها في المعمل حتى + +362 +00:28:28,320 --> 00:28:34,620 +نتجنب ان نطلع نتائج خاطئة في ال microbiology طبعا + +363 +00:28:34,620 --> 00:28:38,080 +انا بقول دايما ان ال soil هي المصدر الأساسي للتربة + +364 +00:28:38,080 --> 00:28:43,180 +لكن من ال soil تعرف ان الهواء بيلامس التربة بياخد + +365 +00:28:43,180 --> 00:28:46,880 +معاها غبار و غبار في أين بيسمع ان؟ الهواء الهواء + +366 +00:28:46,880 --> 00:28:53,910 +لا يوفر بيئةده يعني رطوبة مناسبة أو تغذية مناسبة + +367 +00:28:53,910 --> 00:28:57,690 +تالي هو مجرد carrier للميكروبات حامل للميكروبات + +368 +00:28:57,690 --> 00:29:02,210 +ينقلها من مكان إلى مكان بس التربة فيها مادة عضوية + +369 +00:29:02,210 --> 00:29:06,090 +وفيها رطوبة قد توفر بيه ألمين لنمو المايكرو + +370 +00:29:06,090 --> 00:29:12,270 +أورجانز ال water مش تشتت دنيا تاخد معاها تربة و + +371 +00:29:12,270 --> 00:29:16,070 +تروح عليها بشكل streams و تروح علي نهار أو علي + +372 +00:29:16,070 --> 00:29:19,130 +البحارال animals و ال human bodies احنا فى ال + +373 +00:29:19,130 --> 00:29:24,650 +intestinal tract بتاعنا one gram one gram of stool + +374 +00:29:24,650 --> 00:29:30,250 +contains ten to the power of nine او twelve يعني + +375 +00:29:30,250 --> 00:29:34,010 +عشرة اقص تسعة لعشرة اقص اتناش micro organism ده كل + +376 +00:29:34,010 --> 00:29:38,810 +gram ايش رأيك فى الرقم؟ رقم مهول ال saliva اللى + +377 +00:29:38,810 --> 00:29:43,970 +وعاب تبع الإنسان الواحد ملي منه بيحتوي على عشرة + +378 +00:29:43,970 --> 00:29:48,190 +اقص تسعة micro organism بتاع التقولي فرشية أسناني + +379 +00:29:48,190 --> 00:29:52,310 +مفرشية أسناني أه؟ أصلا الناس المهووسين بأنهم + +380 +00:29:52,310 --> 00:29:55,250 +يفرشوا أسنانهم باستمرار بيصيروا عندهم أمراض يعني + +381 +00:29:55,250 --> 00:29:58,950 +الفم يجب أن يحتوي على micro organism يجب أن يحتوي + +382 +00:29:58,950 --> 00:30:03,430 +على .. مش معناه أضحك أنه نسيبه عفن لأ المقصود فيه + +383 +00:30:03,430 --> 00:30:08,370 +أنه يعني لا مبالغة ولا تتفرض في ال .. فال human + +384 +00:30:08,370 --> 00:30:11,810 +body و ال animal body بتحتوي على micro organism + +385 +00:30:11,810 --> 00:30:17,790 +بعداد ضخمة جداهذا مثال على garden soil بيقولنا + +386 +00:30:17,790 --> 00:30:21,230 +محتوى على تسعة أو سبعة من عشرة ضرب عشرة او اذا كده + +387 +00:30:21,230 --> 00:30:26,410 +شهرين ممكن ستة او سبعة micro organism من التربة + +388 +00:30:26,410 --> 00:30:31,790 +اللي تبعت المنزل طبعا ايش الميزة ما بين تربة و ما + +389 +00:30:31,790 --> 00:30:37,090 +بين انسان او او او انسان مصعب بداية بنفهم مصطلح + +390 +00:30:37,090 --> 00:30:40,950 +كتير مهم اسمه competition الان انتوا في الفصل هنا + +391 +00:30:40,950 --> 00:30:46,170 +لو كنتوا عشر اشخاصفرصتك في انكوا تسألوا سؤال ايه + +392 +00:30:46,170 --> 00:30:50,470 +ليه واحد من عشرة صح ولا لا فرصة اي واحدة منكم طب + +393 +00:30:50,470 --> 00:30:54,250 +لو زاد العدد ساره خمسين بصير فرصتك واحد من خمسين + +394 +00:30:54,250 --> 00:31:01,630 +فالمنافسة بتزيد فبالتالي انت معرفتك بتجل معرفتك + +395 +00:31:01,630 --> 00:31:06,510 +بتجل فوق أقصد فرصك بتجل في ال .. في البيئة + +396 +00:31:06,510 --> 00:31:12,130 +الحقيقية الميكروبات بتكون في حالة تنافس ميكروب + +397 +00:31:12,130 --> 00:31:16,190 +وميكروب وميكروب وميكروببتنفسوا مع بعض وكل مايكروب + +398 +00:31:16,190 --> 00:31:19,490 +فيه استراتيجياته في الدفاع و استراتيجياته في + +399 +00:31:19,490 --> 00:31:25,730 +الهجوم لكن لو واحد من هذه الميكروبات أخدنا لحاله + +400 +00:31:25,730 --> 00:31:30,390 +من المجموعة و حطناها في بيئة مافيش فيها تنافس خرصه + +401 +00:31:30,390 --> 00:31:34,430 +بتزيد او بتزيد اش؟ بتزيد وبالتالي احنا بنحاول برضه + +402 +00:31:34,430 --> 00:31:38,910 +لما بنقزل مايكروب من environment او من clinical + +403 +00:31:38,910 --> 00:31:44,930 +sampleإنه ينشيل عنه الأخرين عشان نوفرله فرصة عالية + +404 +00:31:44,930 --> 00:31:48,170 +فال competition بتكون أقل في الحالة و الفرصة بتكون + +405 +00:31:48,170 --> 00:31:54,290 +أفضل هذه ال .. هذه المادج على إنه أرقام البكتيريا + +406 +00:31:54,290 --> 00:31:58,190 +بتتراوح بحسب المادة اللي موجودة فيها مثلا أنا عندي + +407 +00:31:58,190 --> 00:32:04,570 +أنا cheese ال + +408 +00:32:04,570 --> 00:32:08,970 +milk طبعا ال milk ال raw milk اللي مش مبسطةقد يصل + +409 +00:32:08,970 --> 00:32:13,230 +فيها من 1¹⁸ لكل جرافة لكن بعد ما نبسطره و نبسطره + +410 +00:32:13,230 --> 00:32:16,350 +ليست عملية تعقيم خدوا بالك و انما عملية قتل للبعض + +411 +00:32:16,350 --> 00:32:19,710 +من انك micro organism لذا انا في اندي تراوح و + +412 +00:32:19,710 --> 00:32:24,010 +تثاؤت في ال micro organism من surface إلى surface + +413 +00:32:24,010 --> 00:32:29,930 +طيب قبل ما ابدأ اتكلم باللوحة الفيكوسية اللي قطعت + +414 +00:32:29,930 --> 00:32:33,510 +عنها و ما اتحدث عنها بدنا نحاول الان نرجع إليها + +415 +00:32:34,710 --> 00:32:37,770 +وقولنا ان ال micro organism are with great + +416 +00:32:37,770 --> 00:32:43,670 +biodiversity ايش يعني biodiversity؟ تنوع حيوي + +417 +00:32:43,670 --> 00:32:48,510 +مهمول، بدنا نستغل هذه المعلومة، نعرفها وشو هي تنوع + +418 +00:32:48,510 --> 00:32:51,450 +حيوي وإيش يعني ممكن أو كيف ممكن نستغل هذه + +419 +00:32:51,450 --> 00:32:57,950 +المعلومة؟ احنا على المستوى البشري طعامنا اللي + +420 +00:32:57,950 --> 00:33:01,230 +قدامنا اللي بنشوفه إما نباتات أو حيوانة، صح هذا + +421 +00:33:01,230 --> 00:33:06,900 +لأ؟وطبعا أصناف محددة منه مش كل إشي كده المايكروبات + +422 +00:33:06,900 --> 00:33:11,840 +لما أنا قلت إنها موجودة everywhere في البحر الميت + +423 +00:33:11,840 --> 00:33:16,660 +وفي الكهوف الحمضية وفي الميه أقولكوا أبتدعوا الميه + +424 +00:33:16,660 --> 00:33:21,140 +المادانية اللي بيشرب هذي لما فيها مايكروبات أنا مش + +425 +00:33:21,140 --> 00:33:24,600 +بنقولكوا إن كل .. كل العالم موجود مكتير .. قلتلكوا + +426 +00:33:24,600 --> 00:33:28,760 +قلت 98% من المايكروبات أصلا مش ضار يعني 2% فقط في + +427 +00:33:28,760 --> 00:33:33,370 +الميه 1% من المايكروبات هو اللي ضارلكن وجود + +428 +00:33:33,370 --> 00:33:36,730 +الميكروبات هذه في بيئات متنوعة ومختلفة، ماذا يعني + +429 +00:33:36,730 --> 00:33:43,270 +لكم؟ يعني مقدرة هذه الميكروبات المتنوعة على تناول + +430 +00:33:43,270 --> 00:33:50,190 +أغذية متنوعة طب أقولكم مثال مضطرف جدا، في بكتيريا + +431 +00:33:50,190 --> 00:33:54,710 +متعيش في درجة حرارة قد تصل إلى 200 درجة مأوية، وين + +432 +00:33:54,710 --> 00:33:57,570 +في عصرها الأرض 200 درجة مأوية؟ في الشيء، تحت قاع + +433 +00:33:57,570 --> 00:34:04,000 +المحيطاتبتشوفوا ان كان في ال .. في الوثائقيات اشي + +434 +00:34:04,000 --> 00:34:07,740 +بيطلع من تحت المحيط بيسموها thermal vents فتحات هي + +435 +00:34:07,740 --> 00:34:11,760 +عبارة عن فتحات بوركانية اللي .. اللي بيطلع منها + +436 +00:34:11,760 --> 00:34:15,620 +بيكون طبعا ساخن جدا و لإن المحيط ال .. ال column + +437 +00:34:15,620 --> 00:34:19,400 +تبع الماء عال جدا فبشكل ضغط فالماء قد يصل درجة حرط + +438 +00:34:19,400 --> 00:34:22,460 +هناك فارق المنطقة إلى 200 درجة أو .. أو حتى أعلى + +439 +00:34:22,460 --> 00:34:27,360 +من ذلك ولا يوجد أي نوع من الضوء حتى نقول إن ال + +440 +00:34:27,360 --> 00:34:30,100 +bacterium تم ال photosynthesisاللي هو تمثيل ضوء + +441 +00:34:30,100 --> 00:34:35,860 +وانما تقتات تتغذى على الكابريت الخارج من هذه + +442 +00:34:35,860 --> 00:34:42,060 +الفتحات بس على الكابريت باشي sun light باشي sun + +443 +00:34:42,060 --> 00:34:47,380 +light إذا تنوع التغذية في البكتيريا من نوع إلى آخر + +444 +00:34:47,380 --> 00:34:51,120 +تمام قد يفيدنا كيف بيفيدنا؟ في التعرف و التمييز + +445 +00:34:51,120 --> 00:34:55,840 +بينهمبتستخدمي واحد اتنين تلاتة يا بيستخدم واحد + +446 +00:34:55,840 --> 00:35:00,400 +واربعة وسبعة يا بيستخدم تلاتة واتنين وواحد يبقى + +447 +00:35:00,400 --> 00:35:03,500 +بقدر اميزكوا عن بعض دي الحلقة ابقولكوا كيف الفكرة + +448 +00:35:03,500 --> 00:35:07,440 +تبعها تحت ال microscope اسموا الأسماء اللي بتحكيها + +449 +00:35:07,440 --> 00:35:12,680 +مش للحفظ وانما لالمثال Salmonella type, + +450 +00:35:12,820 --> 00:35:16,580 +Escherichia coli, Sodomonas aeruginosa, Shigella, + +451 +00:35:18,080 --> 00:35:22,410 +Proteus, Morganella, Edward Sella اسمها حلوةبعد + +452 +00:35:22,410 --> 00:35:25,210 +فترة هتعودوا عليها و هتسموا بناتكم زيها في + +453 +00:35:25,210 --> 00:35:31,250 +المستقبل كل الأسماء اللي ذكرتهم و جابتهم متين اسم + +454 +00:35:31,250 --> 00:35:36,550 +تحت الميكروسكوب يبدو و كأنهم شحطة حمراء بقى، زي .. + +455 +00:35:36,550 --> 00:35:44,090 +لونها أحمر و عصايا حمراء كيف نميز إيشريشيا كولار + +456 +00:35:44,090 --> 00:35:46,290 +عن سلمونيلا عن شيجيلا عن الأسماء اللي احنا + +457 +00:35:46,290 --> 00:35:50,860 +ذكرناهم؟نستطيع تمييزهم من خلال ال biodiversity من + +458 +00:35:50,860 --> 00:35:54,020 +خلال تنموها هون في التغيير زى ما قلنا لكوا اشهر + +459 +00:35:54,020 --> 00:35:57,480 +بيخمر ال lactose اشهر بيخمرش ال lactose اشهر + +460 +00:35:57,480 --> 00:36:01,880 +بيستخدم ال glucose اشهر بيستخدم ال sucrose و هكذا + +461 +00:36:01,880 --> 00:36:06,000 +يعني بندير على الفروقات في ال metabolism وهذا + +462 +00:36:06,000 --> 00:36:08,940 +هيشكل جزء كبير من النعمل تبعكم السنة هذه ان شاء + +463 +00:36:08,940 --> 00:36:12,060 +الله هتعرفوا على كيف بنميز ال micro organism + +464 +00:36:12,060 --> 00:36:15,260 +بالفحوصات اللي بيروح كيميائية طبعا هسهرنا برضه ال + +465 +00:36:15,260 --> 00:36:18,860 +ground stateإن ان تشوفوا البكتيريا شحتة حمراء، + +466 +00:36:18,860 --> 00:36:21,820 +ليه؟ لكي تعرفوا أن هذه واحدة من هذه المجموعات، + +467 +00:36:21,820 --> 00:36:23,440 +واحدة من هذه المجموعة. + +468 +00:36:29,420 --> 00:36:33,700 +هذه يمكن اللوحة اللي قدامنا بتوضح لنا دور ال micro + +469 +00:36:33,700 --> 00:36:36,960 +organism في ال nitrogen cycle، nitrogen cycle، + +470 +00:36:36,960 --> 00:36:40,400 +اللي هي دائرة ال nitrogen. أنا بتقولكوا من بداية + +471 +00:36:40,400 --> 00:36:44,980 +المحاضرة إن تدوير العناصر، تدوير العناصر هو الدزء + +472 +00:36:44,980 --> 00:36:49,120 +أساسيمن مهام ال micro organism و أقولكوا جملة + +473 +00:36:49,120 --> 00:36:52,520 +أخبرنا بيها أحد الدكاترات اللي درسونا احنا فيبقى + +474 +00:36:52,520 --> 00:36:56,480 +ننقلها لكم ان شاء الله كلمة لطيفة و لذيذة بتعبر عن + +475 +00:36:56,480 --> 00:36:59,580 +الواقع اللي بنقدر نقصد فيه ال micro organism + +476 +00:36:59,580 --> 00:37:05,080 +بيقولوا لا تظلموا الميكرو بقى هذا المقدمة تبعكوا + +477 +00:37:05,080 --> 00:37:09,020 +لا تظلموا الميكرو بقى their role in nature is to + +478 +00:37:09,020 --> 00:37:13,280 +convert large molecules into small molecules دورها + +479 +00:37:13,280 --> 00:37:16,900 +في الحياةدورة الحياة أن تحول الجزيئات الكبيرة + +480 +00:37:16,900 --> 00:37:21,300 +لجزيئات صغيرة doing their thing يعني doing their + +481 +00:37:21,300 --> 00:37:26,780 +job يعني خلال عملها they may fall in our food and + +482 +00:37:26,780 --> 00:37:32,320 +spoil it قد تقع مش .. مش جاسدها هي بس إذا هي قعدت + +483 +00:37:32,320 --> 00:37:36,200 +وينما .. وينما نحطها ابتدوا ويتكسر انتهى الموضوع + +484 +00:37:36,200 --> 00:37:40,340 +هي دورة الحياة if they enter our body they might + +485 +00:37:40,340 --> 00:37:44,460 +cause diseasesإذا لا تظلموا الميكروفات تعمل عملها + +486 +00:37:44,460 --> 00:37:50,500 +و هي تحويل الجزيئات الكبيرة إلى جزيئات صغيرة فهذه + +487 +00:37:50,500 --> 00:37:54,220 +نقطة كتير مهمة و هذه طبعا أنا مش مش مش كتير مهتمين + +488 +00:37:54,220 --> 00:37:56,820 +بإنه كنا نعرف كيف ال nitrogen سجل لكن في الأخر هي + +489 +00:37:56,820 --> 00:38:01,660 +عملية تحل كنت عارف إن الميكروفات كمان ابتغى من + +490 +00:38:01,660 --> 00:38:05,800 +nitrogen fixation يعني + +491 +00:38:05,800 --> 00:38:10,860 +الزراعة استفادت بشكل كبير من هذه الحقيقةغير + +492 +00:38:10,860 --> 00:38:13,960 +البكتيريا اللى بتكون موجودة في الجذور اللى أصلا + +493 +00:38:13,960 --> 00:38:17,900 +دراستوها نباتات دواة الجذور العقدية اللى بيكون + +494 +00:38:17,900 --> 00:38:22,040 +فيها عقد وهذا العقد أبقى عنها عن بكتيريا عايشة جوا + +495 +00:38:22,040 --> 00:38:25,420 +هذه العقد بعلاقة بيسميها symbiosis اللى هي علاقة + +496 +00:38:25,420 --> 00:38:30,760 +تكافوية بين النبات وبين البكتيريا هناك free living + +497 +00:38:30,760 --> 00:38:34,400 +bacteria ممكن تثبت ال nitrogen لو نميناها في + +498 +00:38:34,400 --> 00:38:38,740 +المختبر ورشيناها على أراضي زراعية مش احنا هو كأنه + +499 +00:38:38,740 --> 00:38:42,750 +أرفنا سماد عضوي عليهايعني بمعنى أخر أن البكتيريا + +500 +00:38:42,750 --> 00:38:48,470 +لها دورة .. دورة عظيمة في هذا الموضوع لكن برضه + +501 +00:38:48,470 --> 00:38:52,850 +شايفين أن هذا .. هذا عبارة عن necrosis قدم للمريض + +502 +00:38:52,850 --> 00:38:59,390 +وفي أن صار فيها اللي هو تحلل أو تاكل أو موضوع إيه + +503 +00:38:59,390 --> 00:39:03,430 +الخلايا فهذا الهرمش بيقولنا not all bacteria cause + +504 +00:39:03,430 --> 00:39:06,550 +disease الوزر أعظم اللي هو قاعة الهرم كلها non + +505 +00:39:06,550 --> 00:39:10,570 +pathogenic غير مفيدةالبعض منها potentially زي ما + +506 +00:39:10,570 --> 00:39:15,570 +قلتلكوا ممكن يمرض ممكن مامرضش والبعض الأخر موصوم + +507 +00:39:15,570 --> 00:39:23,730 +عليه علامة أن هذا دائما ممرض نعرف + +508 +00:39:23,730 --> 00:39:26,250 +كلمة مرة لأ بكافة طب اللي خليني أتكلم عن التعريف + +509 +00:39:26,250 --> 00:39:33,450 +يعني بشكل تفصيل شوية كلمة evolution أحنا قلتلكوا + +510 +00:39:33,450 --> 00:39:38,760 +مش متفقين فيها أن هو موضوع تطورclassification لأ + +511 +00:39:38,760 --> 00:39:41,040 +متفقين في ال classification التصنيف كتير مهم من + +512 +00:39:41,040 --> 00:39:44,800 +التصنيف ليش تصنيف مهم التصنيف مهم قولنالكوا اللي + +513 +00:39:44,800 --> 00:39:51,580 +غرض استرجاع المعلومة طالع لي هنا عندي شخص دخل + +514 +00:39:51,580 --> 00:39:54,820 +الباب و جال دكتور فيه طالب من طالباتك أخدت معاه + +515 +00:39:54,820 --> 00:39:58,560 +مني ورقة و أنا مش هعرفها مش هعرف أي سنة طب + +516 +00:39:58,560 --> 00:40:03,900 +وصفينيها جاتيها طالبة بطلعتكوا بيقولكوا ليه طالبات + +517 +00:40:03,900 --> 00:40:06,060 +فبالتالي + +518 +00:40:07,100 --> 00:40:10,920 +أعطتني معلومة أكدتلي انه انا بدوّف المكان الصحيح + +519 +00:40:10,920 --> 00:40:16,840 +رقم اتنين ايش كمان ماجات لابسها تلبس نضارة الان + +520 +00:40:16,840 --> 00:40:22,140 +انا بدوّف عاملها على لابسين النضارة وبستخني الجميع + +521 +00:40:22,140 --> 00:40:27,600 +لآخر اكمله حضر واحد اتنين تلاتة تلاتة استثنين + +522 +00:40:27,600 --> 00:40:33,320 +الاربع استثنين الباقي طب كمان معلومة بتبقى تعطيني + +523 +00:40:33,320 --> 00:40:38,260 +معلومات لما أصل للشخص الصحيحالان انا شايف good DNA + +524 +00:40:38,260 --> 00:40:43,280 +صح بس ال bacteria بشوفهاش good DNA بشوفها بذاكره + +525 +00:40:43,280 --> 00:40:50,060 +بمعنى بتجمعلها معلومات معلومات نظرية بصفاتها يعني + +526 +00:40:50,060 --> 00:40:51,960 +من اخر الفصل اللي جاي في ال diagnostic + +527 +00:40:51,960 --> 00:40:55,960 +microbiology هتحفظوا إيه شكولايك كرام positive ولا + +528 +00:40:55,960 --> 00:40:59,980 +كرام negative بس اللي ولا كوكسايد إجليكوز negative + +529 +00:40:59,980 --> 00:41:03,040 +ولا إجليكوز positive لا الأكتوز positive ولا + +530 +00:41:03,040 --> 00:41:08,670 +الأكتوز negativeإن دول ميتيل ريدف و بروسكاوه أسماء + +531 +00:41:08,670 --> 00:41:13,950 +عديدة صفات عديدة لميكروب واحد بنخزينهم في وين في + +532 +00:41:13,950 --> 00:41:18,330 +الباكره و بنصير لما نشوفهم بنسترجع و نقارن إذا + +533 +00:41:18,330 --> 00:41:22,010 +أقول إن أنا مش قادر أسترهيب بنستبدلها بإيش ببرامج + +534 +00:41:22,010 --> 00:41:25,750 +حسوب برامج حسوب بندخله البياتة بدء من الحفاظ + +535 +00:41:25,750 --> 00:41:29,190 +اللحظة بندخلها بالحسوب و لما بيجينا ميكروب يقولي + +536 +00:41:29,190 --> 00:41:33,170 +بندخله البياتة صفاته بقول your microbe is كده و + +537 +00:41:33,170 --> 00:41:37,380 +كده وصل الصورةفعملية التعرف للميكروهيزم بتتم + +538 +00:41:37,380 --> 00:41:41,740 +بالاستثناء لكن بالأساس معظم العالمين على ال + +539 +00:41:41,740 --> 00:41:45,260 +classification كلمة morphology اللي هو ده اعتماد + +540 +00:41:45,260 --> 00:41:47,980 +الشكل هو احنا بقولك البكتيريا very simple ياما + +541 +00:41:47,980 --> 00:41:52,480 +عصوية ياما كراوية ياما الهي اسباية physiology + +542 +00:41:52,480 --> 00:41:56,320 +التغذية تبعتها أنا بترجع بشوية كيف تتغذى و كيف + +543 +00:41:56,320 --> 00:42:00,960 +تنمو التغذية تبعتها هل تستخدم ال substrate X ولا + +544 +00:42:00,960 --> 00:42:06,140 +ال substrate Y الاخرىالـ Genetics تبعتها بعض + +545 +00:42:06,140 --> 00:42:12,700 +الأحيان .. طبعا هنا قلنا قبل شوية أنه هناك تغيير + +546 +00:42:12,700 --> 00:42:16,360 +مستمر في التصنيف تبع الميكروبات مستثمر في درجة أن + +547 +00:42:16,360 --> 00:42:21,360 +معروفاتنا بتزيد في السنوات الأخيرة علم الوراطة + +548 +00:42:21,360 --> 00:42:26,080 +الجزائية قفز قفزات نوعية وبالتالي بالفعل ساعدنا في + +549 +00:42:26,080 --> 00:42:29,060 +عملية اللي هو التصنيف مرة أخرى ال ecology البيئة + +550 +00:42:29,060 --> 00:42:32,480 +تبعة الميكروبات under certain definite conditions + +551 +00:42:32,600 --> 00:42:38,080 +تحت ظروف اللي هو معين تاني كمان هنا شغل تانية the + +552 +00:42:38,080 --> 00:42:43,580 +law of their life activities ما النظام اللي يسيطر + +553 +00:42:43,580 --> 00:42:47,940 +أو يحكم تصرف اللي هو ال micro organism and their + +554 +00:42:47,940 --> 00:42:51,520 +interaction with the human being كيف نتفاعل مع + +555 +00:42:51,520 --> 00:42:58,610 +البشرسلبا، إيجابا، فائدة، mutualism يعني اتفاد + +556 +00:42:58,610 --> 00:43:03,210 +منفعة و لا harmful relation هذا كلها ضمن ال micro + +557 +00:43:03,210 --> 00:43:07,030 +environment طبعا interaction with human beings + +558 +00:43:07,030 --> 00:43:10,270 +animates our plan as well as natural environment + +559 +00:43:10,270 --> 00:43:18,890 +انتوا بتعرفوا انه كتير من البيئات فسدت بسبب طمع + +560 +00:43:18,890 --> 00:43:24,520 +البشر خدوها قاعدة أينما وجد الماءعلى .. تاريخيا + +561 +00:43:24,520 --> 00:43:28,560 +استوطن الناس .. استوطن الناس و بتعرف كل الحضارات + +562 +00:43:28,560 --> 00:43:34,100 +نجحت على الأوضيه و الأنهار و أينما حل الإنسان و + +563 +00:43:34,100 --> 00:43:38,880 +استوطن حل الفساد الشيء كان إيه؟ لإنه بيطلع البحر + +564 +00:43:38,880 --> 00:43:41,520 +.. قطع الميه .. بيجيه الميه جارية .. بيصيب + +565 +00:43:41,520 --> 00:43:45,080 +النفايات تبعته يحطها .. ماشي النفايات الميه + +566 +00:43:45,080 --> 00:43:48,220 +الجارية هيخدها للتطبيق المعين .. لكن لإن حجم + +567 +00:43:48,220 --> 00:43:54,160 +النفايات بيزيدبنسميها زاد أو فاق عن قدرة الـ self + +568 +00:43:54,160 --> 00:43:57,080 +-purification ليها التنقية الذاتية، ليها ده بيسب + +569 +00:43:57,080 --> 00:44:01,200 +من الماء وبالتالي بيفسد، وبالتالي بيفسد فال + +570 +00:44:01,200 --> 00:44:06,440 +interaction ما بين الإنسان و ال environment قد + +571 +00:44:06,440 --> 00:44:09,460 +يكون واصل بينهم ال micro organism يعني كتير من + +572 +00:44:09,460 --> 00:44:14,340 +البيئة استرجعناها بعد ما فسدت باستخدام micro + +573 +00:44:14,340 --> 00:44:19,060 +organism بنحط فيها micro organism خلطات معينةكي + +574 +00:44:19,060 --> 00:44:23,440 +بتسترجعنا البيئة، بتكسر المواد الضارة، بتشيل بعض + +575 +00:44:23,440 --> 00:44:29,700 +السموم من البيئة، وبالتالي بنرجع البيئة بشكل جيد + +576 +00:44:29,700 --> 00:44:35,420 +أنا قلت انه كتير ميكروباليجي مهمة في حياتنا، مهمة + +577 +00:44:35,420 --> 00:44:38,160 +في ال environment، مهمة في الطب، غير انها بتسببنا + +578 +00:44:38,160 --> 00:44:43,560 +امراض، علاجات، بنفلق منها، في ال food الإنسان عرف + +579 +00:44:43,560 --> 00:44:48,280 +الميكروبات، خدوا بالكوا، عرف الميكروبات قبل العلم + +580 +00:44:48,280 --> 00:44:52,840 +الحديدالمصريين القدامى لا عندهم microscope ولا + +581 +00:44:52,840 --> 00:44:56,800 +عندهم أي أجزة من الأجزة اللى .. اللى موجودة و بعد + +582 +00:44:56,800 --> 00:45:00,440 +ذلك صنعوا الخبز و الخبز معروف أنه بتدخل بصناعته + +583 +00:45:00,440 --> 00:45:04,840 +مين الخميرة و الخميرة micro organism و لحد وقت + +584 +00:45:04,840 --> 00:45:10,120 +قريب كانوا يعني مش .. مش أجدادكوا يعني أباهم .. + +585 +00:45:10,120 --> 00:45:14,580 +أبا أجدادكوا كانوا العجين اللى بيعجنوه في البيت مش + +586 +00:45:14,580 --> 00:45:20,180 +بيشتروه خميرة بيخلوا جزء من عجين مباركتمام؟ و + +587 +00:45:20,180 --> 00:45:25,960 +بخلطوه مع عجين بكرة يعني بياخدوا الخميرة لمن؟ بنمو + +588 +00:45:25,960 --> 00:45:29,780 +الخميرة من عليه نفسه هذا بيصير مصدر الخميرة بيحطوه + +589 +00:45:29,780 --> 00:45:32,840 +ل .. ل .. ل .. فهذا الكلام قبل ما يعرفوا حاجة من + +590 +00:45:32,840 --> 00:45:36,980 +ال micro بيقولوا ان المصريين القدماء استخدموا + +591 +00:45:36,980 --> 00:45:42,500 +الخبز لمعفن تمام؟ لمدوّة الجروح العفن هذا هو + +592 +00:45:42,500 --> 00:45:48,500 +penicillin يبقى الإنسان شعر بي ..قيمة ال micro + +593 +00:45:48,500 --> 00:45:52,020 +organism قبل حتى اللي هو الاتشار في العلم الحديث + +594 +00:45:52,020 --> 00:45:55,380 +عن ال micro bio في الفوق ال industry ال bio + +595 +00:45:55,380 --> 00:45:58,840 +technology و ال research كلمة bio technology تسمع + +596 +00:45:58,840 --> 00:46:04,900 +ان فينا جسم هنا في ال GMO اسمه bio technology عشان + +597 +00:46:04,900 --> 00:46:09,160 +انا اقولكم جداش مهم ال micro vat في ال bio + +598 +00:46:09,160 --> 00:46:11,220 +technology فيه فرق في ال bio technology اسمه + +599 +00:46:11,220 --> 00:46:15,140 +industrial microbiome industrial microbiome جزء من + +600 +00:46:15,140 --> 00:46:20,130 +مين؟ من ال bio technologyيقدر ال industrial + +601 +00:46:20,130 --> 00:46:23,010 +microbiology investment اللي هو الاستثمار اللي + +602 +00:46:23,010 --> 00:46:27,330 +قاعد فيه في العالم ببلايين الدولارات سنوية فقطعهم + +603 +00:46:27,330 --> 00:46:31,450 +هفر ده طبعا ال research دايما بيقولوا في الطب the + +604 +00:46:31,450 --> 00:46:34,990 +research of today is the medicine of tomorrow + +605 +00:46:34,990 --> 00:46:38,570 +يعنيش معناها معناه يقولوا بيه البحث اليوم بيصير + +606 +00:46:38,570 --> 00:46:43,170 +الطب .. بيصير الطب لل .. لل .. ده نموذج على تصنيع + +607 +00:46:43,170 --> 00:46:47,850 +نستكلم عنهأنا قلت ممكن يوجهوا شوية لقصة طرفنا اللي + +608 +00:46:47,850 --> 00:46:52,170 +له حق بالطريق إن الإنسان شعر بي ال .. ال .. + +609 +00:46:52,170 --> 00:46:57,430 +الميكروبات و استخدمها قبل ما يعرفها و بعدين بدأ ي + +610 +00:46:57,430 --> 00:47:01,490 +.. يتعرف عليها و الآن في أنها طور بالفعل modern + +611 +00:47:01,490 --> 00:47:05,570 +جدا و متعامل مع .. إن احنا متعامل مع الميكروبات و + +612 +00:47:05,570 --> 00:47:12,550 +كأنها دواجن بننميها أو كأنها أبقار بنربيهابنتعامل + +613 +00:47:12,550 --> 00:47:17,430 +معها بطرق قياسية سهلة جدا و رخيصة جدا و بنستغرب + +614 +00:47:17,430 --> 00:47:21,910 +منها بشكل كام من + +615 +00:47:21,910 --> 00:47:26,650 +الناس اللي أثروا علم ال microbiology Anthony van + +616 +00:47:26,650 --> 00:47:32,030 +Leeuwenhoek اللي اخترع اللي هو ال microscope و + +617 +00:47:32,030 --> 00:47:37,770 +Louise Pastier اللي كان البسطر على اسمه تمام و + +618 +00:47:37,770 --> 00:47:43,770 +Robert Koch اللي عمل فرضيةكوخ سموها كوخ postulate + +619 +00:47:43,770 --> 00:47:47,410 +هتكلم عنها انشاء الله لاحقا بليستر اللي هو اتكلم + +620 +00:47:47,410 --> 00:47:50,950 +على ال sterilization of this infection اللي هو + +621 +00:47:50,950 --> 00:47:56,310 +عامل تعقيمه في التطهير هذه طبعا بعض الشخصيات اللي + +622 +00:47:56,310 --> 00:47:58,870 +انا ذكرتها هذا Anthony van Leeuwenhoek وهي ال + +623 +00:47:58,870 --> 00:48:06,650 +microscope تبعه اللي هو اللي عمله هذا + +624 +00:48:06,650 --> 00:48:11,820 +مين؟ هذا Edward JennerEdward Jenner هذا اللي كان + +625 +00:48:11,820 --> 00:48:15,620 +مسئول عن تطوير اللقاحات و ال vaccines اللي احنا + +626 +00:48:15,620 --> 00:48:19,020 +بنعرفها او التطييمات اللي بنعرفها في العصر الحديث + +627 +00:48:19,020 --> 00:48:23,220 +طبعا التطييمات كانت موقفة سابقا الصينيين عرفوها و + +628 +00:48:23,220 --> 00:48:28,540 +العرب عرفوها لكن ماعرفوهاش في طور المدن ولا في ال + +629 +00:48:28,540 --> 00:48:31,600 +experimental عرفوها في ال pre اللي هي المرحلة + +630 +00:48:31,600 --> 00:48:33,860 +الأولى اللي قلنالكوا عملنا خبز و احنا مش عارفين + +631 +00:48:33,860 --> 00:48:39,780 +ليش الخبز صار هيك نفس ال case كان فيهحاجة لطيفة في + +632 +00:48:39,780 --> 00:48:44,140 +السعودية و العراق في مرة اسمه Baghdad Sour قرحة + +633 +00:48:44,140 --> 00:48:47,760 +بغداد .. قرحة بغداد هنا فيها ريحة مولودة في في + +634 +00:48:47,760 --> 00:48:52,640 +فلسطين عبارة عن ذبابة بتعض ال .. ال .. ال .. + +635 +00:48:52,640 --> 00:48:56,180 +السابق تنقله برازايد اسمه Leishmania هان ال + +636 +00:48:56,180 --> 00:49:00,480 +Leishmania مشهورة في اليمن و السعودية و العراق لما + +637 +00:49:00,480 --> 00:49:06,020 +تصيب الفتاة في الوجه مثلا تعملها بجعة دائرية كويس + +638 +00:49:06,020 --> 00:49:11,450 +trademark علامةخلاص هذه بتشوه اللي هو الشكل فكانت + +639 +00:49:11,450 --> 00:49:16,390 +السيدات اللي ومات يروحوا على واحدة مصابة وواحد + +640 +00:49:16,390 --> 00:49:20,570 +مصاب ياخدوا منه ويحقنوا بناتهم في مناطق .. في + +641 +00:49:20,570 --> 00:49:25,670 +مناطق من الدسم غير .. يغير الوجهة فكان عمال يتجلى + +642 +00:49:25,670 --> 00:49:29,250 +انتصاب بيها مرة واحدة وابتدا الحفرة في المكان اللي + +643 +00:49:29,250 --> 00:49:33,730 +يبين عنه غير ظاهر لكن تضمن انه مايصيبش الوجهة وهذه + +644 +00:49:33,730 --> 00:49:37,520 +كانت برضه من اللي هو عملية ال vaccinationاللي + +645 +00:49:37,520 --> 00:49:43,540 +أُخدت بالـ Experience هذا + +646 +00:49:43,540 --> 00:49:48,860 +الـ A. Louis Pasteur تميز + +647 +00:49:48,860 --> 00:49:54,320 +عشان أميزكم بين العلماء + +648 +00:49:54,320 --> 00:50:01,840 +كان رأسه مزدحم بالأفكار بمعنى أنه كان يقترح و عنده + +649 +00:50:01,840 --> 00:50:06,460 +أفكار يقوله ألف فكرة في الشهر تصبح منها فكرة واحدة + +650 +00:50:06,460 --> 00:50:17,920 +من الألفبعكس أزميله Robert Koch Robert + +651 +00:50:17,920 --> 00:50:25,400 +Koch كان hard-headed عنيد يعني لا يقفنق بسهولة + +652 +00:50:25,400 --> 00:50:30,300 +تقرا ورا حده بضل وجهه وراه لم يخلصها وكان من نتائج + +653 +00:50:30,300 --> 00:50:33,500 +أعماله كنا فرضية Koch اللي هتطلقنا إن شاء الله + +654 +00:50:33,500 --> 00:50:37,060 +المحاضرة اللي جاية وهي فرضية رائعة جدامن خلالها + +655 +00:50:37,060 --> 00:50:40,800 +أثبت أن هذا الميكروب هو المسبب لهذا المرض انا + +656 +00:50:40,800 --> 00:50:43,920 +أعتقد مرض عليه ممكن فيه حاجة او شيء فهنطلق لها ان + +657 +00:50:43,920 --> 00:50:52,540 +شاء الله في + +658 +00:50:52,540 --> 00:50:57,040 +عالم اسمه Dimitry Ivanovsky هو عالم روسي هو اللي + +659 +00:50:57,040 --> 00:51:01,300 +اكتشف الفيروسات و اكتشاف الفيروسات كان بطريقة يعني + +660 +00:51:01,300 --> 00:51:05,040 +فيها نوع من المصادقة انه كان بيحاول ان ي filter + +661 +00:51:05,480 --> 00:51:11,180 +العمل المسبب لمرض تبرقش الدخان، الدخان اللي هو ال + +662 +00:51:11,180 --> 00:51:16,020 +tobacco، tobacco فكان + +663 +00:51:16,020 --> 00:51:19,400 +يميل عبر المرشحات البكتيرية و ياخد السائل و يروشه + +664 +00:51:19,400 --> 00:51:22,500 +على النباتي، عوض النباتي الصعب فجال إن العامل + +665 +00:51:22,500 --> 00:51:28,360 +المسبب لتبرقش أوراق ال tobacco هو filterable، + +666 +00:51:28,360 --> 00:51:31,400 +filterable يعني قارب .. يعني بدخل من .. يعني أنا + +667 +00:51:31,400 --> 00:51:35,210 +حاجة وزرمةوبعدين اهيب هو ان هو المرة جاى ان شاء + +668 +00:51:35,210 --> 00:51:38,350 +الله نكمل مقدماتنا ونبدأ بمحاضرة اول ان شاء الله + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m_if8j49Njk_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m_if8j49Njk_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..b755a9a98fe64d859625c15a9bfb9957f2f02c0e --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/m_if8j49Njk_raw.srt @@ -0,0 +1,2436 @@ +1 +00:00:20,210 --> 00:00:24,670 +بسم الله الرحمن الرحيم المحاضرة السابقة بدأنا + +2 +00:00:24,670 --> 00:00:29,770 +بالحديث عن ال Vibrio و اليوم هتكلم عن مجموعة اسمها + +3 +00:00:29,770 --> 00:00:30,230 +Sodomona + +4 +00:00:35,180 --> 00:00:42,340 +سودامونس نفسها و المجموعة اللى تنتمى إلها لا يربط + +5 +00:00:42,340 --> 00:00:48,360 +بينها يعنى كتير من الصفات لكن عنوان أساسى إنها non + +6 +00:00:48,360 --> 00:00:52,560 +-fermenter بمعنى إنهم كلهم بيخمروش لجلوكوز غير + +7 +00:00:52,560 --> 00:00:54,180 +قادر على تخمير الجلوكوز + +8 +00:01:05,810 --> 00:01:10,310 +وراق لجلة، وراق لجلة، الفرق بين الاتنين هنا أنه + +9 +00:01:10,310 --> 00:01:13,550 +بلد ما هي curved، البس الله هنا ايه؟ straight، + +10 +00:01:13,550 --> 00:01:17,630 +يعني مافيش فيها الانحلاق اللي موجود في ال .. ال + +11 +00:01:17,630 --> 00:01:21,760 +vibeالgeneral characteristics لهذه المجموعة أنها + +12 +00:01:21,760 --> 00:01:25,160 +opportunistic pathogens of plants, animals and + +13 +00:01:25,160 --> 00:01:28,720 +humans بينما لما تخلقنا على الانترو بكتيرياسي ما + +14 +00:01:28,720 --> 00:01:32,400 +ذكرنا ال plants، ال animals لأ، في أشياء مشترك، + +15 +00:01:32,400 --> 00:01:35,980 +وخدوا بالكوا من كلمة مشترك، تسمعوا بمصطلح zoonotic + +16 +00:01:35,980 --> 00:01:40,160 +diseases، zoonotic diseases هي الأونات المشتركة، + +17 +00:01:40,160 --> 00:01:46,040 +بتصيب الحيوانأو العكس بتصيب الإنسان و بتنتقل كمان + +18 +00:01:46,040 --> 00:01:49,060 +لمين؟ للحيوان، يعني في صنفين، في منتقل من الحيوان + +19 +00:01:49,060 --> 00:01:53,200 +للإنسان و من الإنسان للحيوان لكن في النهاية نسبة + +20 +00:01:53,200 --> 00:01:57,380 +تتصل إلى أكتر من 60% من الأمراض المعدية هي أمراض + +21 +00:01:57,380 --> 00:02:02,260 +مشتركة و لا تنسوا إن أحنا بنعيش في عالم غير بعيدين + +22 +00:02:02,260 --> 00:02:05,880 +عن الحيوان، كيف؟ و أحنا عايشين في مدن في بعض + +23 +00:02:05,880 --> 00:02:09,160 +الأحيان، في شقة تعالوا تطلعوا على إيش احتكاك + +24 +00:02:09,160 --> 00:02:14,540 +الإنسان بالحيوانأول احتكاك بنتكلم عنه كلكم تعرفوه + +25 +00:02:14,540 --> 00:02:20,700 +كغذاء صح؟ و بما أنه غذاء .. بما أنه غذاء إذا ينمى + +26 +00:02:20,700 --> 00:02:24,660 +و يباع في الأسواق .. شوفوا لإن ينمى يعني يستزرع مش + +27 +00:02:24,660 --> 00:02:29,600 +بيقولوا مزارع، يستزرع الدواجن ففي ناس بتربي دواجن، + +28 +00:02:29,600 --> 00:02:32,580 +في ناس بتنقل دواجن، في ناس بتبيع دواجن، في ناس + +29 +00:02:32,580 --> 00:02:34,900 +بتمظف دواجن أو في ناس بتاكل + +30 +00:02:38,240 --> 00:02:42,920 +انه فى حلقة كبيرة من السكان اللى احتكاك بال .. بال + +31 +00:02:42,920 --> 00:02:47,280 +.. بالحياة من ناحية غذائى نجى الان من ناحية اللى + +32 +00:02:47,280 --> 00:02:51,880 +هو ال .. ال bits اللى هم الحياة الأليفة البعض + +33 +00:02:51,880 --> 00:02:56,460 +بيقتنى قطط فى المنازل صح ولا لأ البعض بيقتنى طيور + +34 +00:02:56,460 --> 00:03:01,820 +لزينا عصفر ببغاء دماء وحطه دجافة وعنده فى البيت + +35 +00:03:01,820 --> 00:03:08,530 +وعصفور و الببغاء و القطط قد تنقل الأمراضالاحتكاك + +36 +00:03:08,530 --> 00:03:14,590 +التالت اللي هي القوارض المنتشرة، الفقران والجرذان + +37 +00:03:14,590 --> 00:03:19,410 +اللي موجودة بكثرة في بعض المناطق، قد تصل إلى يعني + +38 +00:03:19,410 --> 00:03:23,930 +البيوت معظم الناس، لأن هناك احتكاك قوي ما بين + +39 +00:03:23,930 --> 00:03:26,390 +الإنسان وما بين الحيوانات بتلت أشكال، الشكل الأول + +40 +00:03:26,390 --> 00:03:29,750 +بيقولنا كده مرة، اللي هو الغذاء، الشكل التاني، + +41 +00:03:29,750 --> 00:03:33,240 +حيالة القليفة، الشكل التالت اللي هواللي هو القوارب + +42 +00:03:33,240 --> 00:03:36,200 +او الحيوانات المنتشرة في مثلا الكلاب الضالة ايضا + +43 +00:03:36,200 --> 00:03:41,220 +كمان هي تتبر من الحيوانات المزايدة في احتكاك رابع + +44 +00:03:41,220 --> 00:03:44,440 +وخالم وثالث لكن لفئات معينة من الناس مثلا الطباء + +45 +00:03:44,440 --> 00:03:48,100 +البيطاريين اللي لهم خصية صح الناس اللي بيشتغلوا في + +46 +00:03:48,100 --> 00:03:53,180 +هدفة الحيوان هدولهم خصية احنا بيقولنا المربين + +47 +00:03:53,180 --> 00:04:00,040 +هدولة شملناهم في موضوع المزارة طيبإذن إمكانية + +48 +00:04:00,040 --> 00:04:03,020 +انتقال الميكروبات من الحيوان للإنسان أو من الإنسان + +49 +00:04:03,020 --> 00:04:06,540 +للحيوان الموجودة حتى في المجتمع المدني وإن يعني + +50 +00:04:06,540 --> 00:04:11,420 +رؤيتنا للحيوانات الحية قليل لكن بنتعامل معها كغدا + +51 +00:04:11,420 --> 00:04:15,780 +على الأقل clinically important aerobic gram + +52 +00:04:15,780 --> 00:04:18,660 +negative بس الله لأ كلمة aerobic محورية في + +53 +00:04:18,660 --> 00:04:22,940 +المحاضرة تبعتنا اليوم كلمتين محورية non-fermented + +54 +00:04:22,940 --> 00:04:26,810 +و non-aerobicلأنه ايش جاب ال pseudomonas + +55 +00:04:26,810 --> 00:04:30,810 +aeruginosa للاسنيتوباكتر؟ هذه واحدة على الطرف هذا + +56 +00:04:30,810 --> 00:04:34,690 +كانت في الداجلان فاكرين؟ في ال oxidase، اللي فصلهم + +57 +00:04:34,690 --> 00:04:38,290 +ال oxidase، لكن لو طلعنا على وين مقرهم في ال + +58 +00:04:38,290 --> 00:04:42,350 +glucose fermentation عليهم نفس المكان، both are + +59 +00:04:42,350 --> 00:04:45,170 +non-fermental، إذن الصفة الأساسية بالفعل إنهم non + +60 +00:04:45,170 --> 00:04:49,750 +-fermental، رقم اتنين إنهم ايروبي، وطبعا يعني أتقد + +61 +00:04:49,750 --> 00:04:54,230 +إن هذي بتأدي لهذه، هذي بتأدي لهذه، صح ولا بقى؟و + +62 +00:04:54,230 --> 00:04:56,910 +طبعا بتقدي لجنود تانية هتعرفها كمان شوية أنه لازم + +63 +00:04:56,910 --> 00:05:00,150 +تكون catalyse postive مثلا لازم تكون .. مش لازم + +64 +00:05:00,150 --> 00:05:02,410 +تكون oxalyse postive لكن catalyse لازم تكون + +65 +00:05:02,410 --> 00:05:06,790 +postive أسماء مهمة Sodomonas aeruginosa + +66 +00:05:06,790 --> 00:05:11,750 +borcholderia حلوا اسمها ده borcholderia sebacia و + +67 +00:05:11,750 --> 00:05:14,350 +لو درستوا قبل سنتين أو تلاتين مع الدكتور عبد + +68 +00:05:14,350 --> 00:05:16,270 +القادر اللي قدر كان جالكوا Sodomonas sebacia .. + +69 +00:05:16,270 --> 00:05:19,150 +sebacia لأن اسم القديم اللي كان Sodomonas sebacia + +70 +00:05:19,150 --> 00:05:23,910 +جديد سميت borcholderiaالاسم التالت طويل شوية + +71 +00:05:23,910 --> 00:05:28,030 +بلانجته استينوتروفوموناص + +72 +00:05:28,030 --> 00:05:35,330 +مالتوفيلا استينوتروفوموناص مالتوفيلا الرابع + +73 +00:05:35,330 --> 00:05:40,630 +أسينيتوبكتر بوماني الخامس موراجزيلا كاتيراز أسماء + +74 +00:05:40,630 --> 00:05:43,130 +طبعا خدوا بالكم أنا كمان مرة في ال medical + +75 +00:05:43,130 --> 00:05:47,990 +microbiology لا نقشت الانتروباترياس كلهم ولا نقشت + +76 +00:05:47,990 --> 00:05:51,650 +ال Vibrio حتى كمان كلها أنا نقشت جزئية منهاأنا + +77 +00:05:51,650 --> 00:05:55,750 +بحاول أستعرض أمامكوا بعض النقاط اللي هدرسهم + +78 +00:05:55,750 --> 00:05:59,850 +بتفاصيل أكبر في وين ال diagnostic microbiology لكن + +79 +00:05:59,850 --> 00:06:03,610 +بوديكموا نماذج ان كيف احنا ممكن نستفيد من ال + +80 +00:06:03,610 --> 00:06:05,690 +medical microbiology ل ال diagnostic microbiology + +81 +00:06:05,690 --> 00:06:09,530 +خدوا بالكم البعض بيقولوا ال microbiology هي course + +82 +00:06:09,530 --> 00:06:13,890 +حفظ لازم نحفظ المعلومة انا لا اريد ان اجادلكم في + +83 +00:06:13,890 --> 00:06:17,610 +هذا لا اريد ان اجادلكم في انها it requires + +84 +00:06:17,610 --> 00:06:24,480 +memorizationلكن ولا واحد حفظ ال microbiology فقط و + +85 +00:06:24,480 --> 00:06:28,340 +فلح انه يصير microbiologist لازم يكون عنده مقدرة + +86 +00:06:28,340 --> 00:06:34,140 +على انه يربط المعلومات اللي حفظها مع بعض و هو + +87 +00:06:34,140 --> 00:06:38,480 +التعريف الحقيقي لل science واحد جال التعريف العلم + +88 +00:06:38,480 --> 00:06:43,360 +جال it is not learning a bunch of facts ليس العلم + +89 +00:06:43,360 --> 00:06:48,330 +هو عملية تعلم حفنة من الحقائقناخدها من المدرس و + +90 +00:06:48,330 --> 00:06:53,030 +نرجع نرددلها تاني تمام؟ بنعرف هذا الحقائق ليس + +91 +00:06:53,030 --> 00:06:57,290 +learning a bunch of facts it is a systematic + +92 +00:06:57,290 --> 00:07:03,990 +process عملية نظامية بجمع الحقائق و بربطها ببعض + +93 +00:07:03,990 --> 00:07:09,690 +حتى أصل applied حاجة تطبيقية حاجة تطبيقية أستفيد + +94 +00:07:09,690 --> 00:07:13,770 +منها وبالتالي مطلوب مننا نحفظناها زي واحد بيقولك + +95 +00:07:13,770 --> 00:07:19,020 +انابحبش الحفظ لكن بفهم، بنقولله كيف يعني مثلا + +96 +00:07:19,020 --> 00:07:21,820 +أحافظ نظريات في سجول و أقولها فاهمها، بقولك أنا + +97 +00:07:21,820 --> 00:07:25,180 +فاهمها، طب قولنا إياها، كم بيشرحلنا إياها، طب ما + +98 +00:07:25,180 --> 00:07:28,880 +هو شرحك اللي هدل علينا كده، حافظها، وبتالي ال .. + +99 +00:07:28,880 --> 00:07:31,860 +تنتين ما بيجب أن تجزّيهم مع بعض، الحفظ مهم لعملية + +100 +00:07:31,860 --> 00:07:36,460 +برضه إيه الفهم، في كثير من الأحيان، و أهم من ذلك + +101 +00:07:36,460 --> 00:07:37,180 +دليل التحقق + +102 +00:07:41,870 --> 00:07:43,990 +General characteristics of non-fermenters, + +103 +00:07:44,230 --> 00:07:46,310 +oxidative-gram-negative bacilli including + +104 +00:07:46,310 --> 00:07:49,310 +pseudomonas species produces acid from glucose or + +105 +00:07:49,310 --> 00:07:53,550 +other carbohydrates only in the presence of oxygen + +106 +00:07:53,550 --> 00:07:59,310 +تعالى الأن نسأل سؤال للـ quiz الجاى ونقول true or + +107 +00:07:59,310 --> 00:08:04,710 +false pseudomonas aeruginosa is glucose positive + +108 +00:08:04,710 --> 00:08:05,910 +صحة لغلط + +109 +00:08:11,040 --> 00:08:14,720 +أنا قلت fermentation، قلت جلوكوز positive، لا قلت + +110 +00:08:14,720 --> 00:08:17,820 +fermentation ولا قلت أي حاجة تانية، قلت جلوكوز + +111 +00:08:17,820 --> 00:08:25,020 +positive طيب، أنا بقولكوا true، و أنا صحي و أنتوا + +112 +00:08:25,020 --> 00:08:30,760 +صح، إذا أنا اتفرجتها من المنظور إنه it is جلوكوز + +113 +00:08:30,760 --> 00:08:36,210 +oxidizer positive صحوزي انتوا قدرتولها من منظور ال + +114 +00:08:36,210 --> 00:08:39,690 +glucose fermentation انتوا صح طب من أي منظور من + +115 +00:08:39,690 --> 00:08:45,710 +منظورها انتوا معايا عندنا احتماليتين او تلت + +116 +00:08:45,710 --> 00:08:48,990 +احتمالات للتعامل مع ال carbohydrate بالنسبة + +117 +00:08:48,990 --> 00:08:52,040 +للبكتيرياشايفين الأنبوبة اللي على الصبورة اللي + +118 +00:08:52,040 --> 00:08:56,840 +أمامنا؟ فيها أنبوبتين زي بعض زرعناهم بنفس .. اللي + +119 +00:08:56,840 --> 00:08:58,960 +هو أنبوبتين أفضل نفس ال media زرعناهم بنفس + +120 +00:08:58,960 --> 00:09:02,460 +البكتيريا بنفس البكتيريا واحدة منهم حاطين على ال + +121 +00:09:02,460 --> 00:09:06,940 +top زي ما أنتوا شايفين الزيت والتانية بدون زيت، + +122 +00:09:06,940 --> 00:09:10,220 +الزيت مش عمل .. عمل طبقة عازلة حتى يمنع ال oxygen + +123 +00:09:10,220 --> 00:09:15,800 +عن ال mediaالان لو البكتيريا بكتيريا لا هوائية .. + +124 +00:09:15,800 --> 00:09:19,680 +لا هوائية أو أنت المقدر تعمل fermentation أكيد + +125 +00:09:19,680 --> 00:09:22,360 +هتستغل ال .. ال glucose اللي مورود في اليوم بوبا + +126 +00:09:22,360 --> 00:09:25,900 +اللي فيها زيت و تغير لون الوصلة صح ولا لأ؟ يبقى + +127 +00:09:25,900 --> 00:09:28,700 +هيك بتكون أشياء fermented .. ف .. خلانا نقول + +128 +00:09:28,700 --> 00:09:31,460 +fermented .. بدنا .. بدنا أصلا كلمة بستر و نجد + +129 +00:09:31,460 --> 00:09:34,960 +بدنا نغير رفوم اليومفي الموضوع التاني، لو + +130 +00:09:34,960 --> 00:09:38,140 +البكتيريا غيرت اللون، وإذا ملاحظين أنه غير اللون، + +131 +00:09:38,140 --> 00:09:41,420 +وين مغير اللون؟ على السطح، دلوقت أنا أبدأها إيه؟ + +132 +00:09:41,420 --> 00:09:46,780 +إيه الهواء، يفجأة هي عملت oxidation للكربوهيدرات + +133 +00:09:46,780 --> 00:09:48,960 +هناك، واحدة عملت oxidation، واحدة عملت + +134 +00:09:48,960 --> 00:09:52,860 +fermentation، لو عملت في اتنين، بتكون oxidative، + +135 +00:09:52,860 --> 00:09:56,700 +fermentative، يعني البكتيريا facultative، أنا ايضا + +136 +00:09:56,970 --> 00:10:00,950 +لو عملت فقط في الهوائية فهي oxidative لو عملت في + +137 +00:10:00,950 --> 00:10:03,970 +اللي فيها زيت بتكون أنا aerobic و بتعمل + +138 +00:10:03,970 --> 00:10:07,310 +fermentation إذا الأن كيف بدنا نفسر نتائج طبعتنا + +139 +00:10:07,310 --> 00:10:11,290 +لما نقول جليكوز positive ولا جليكوز negative لازم + +140 +00:10:11,290 --> 00:10:14,270 +نكون عارفين السؤال بتكلم عن oxidation ولا عن مين + +141 +00:10:14,270 --> 00:10:21,090 +عن fermentation طيب في حل تالت الحين البكتيريا لما + +142 +00:10:21,090 --> 00:10:25,730 +أكثرت الجليكوز إيش يعني أكثرته استغلته؟لما عملتله + +143 +00:10:25,730 --> 00:10:29,330 +fermentation ايش يعني؟ يعني استغلته في مصطلح اسمه + +144 +00:10:29,330 --> 00:10:32,790 +glucose utilization او carbohydrate utilization ال + +145 +00:10:32,790 --> 00:10:37,770 +utilization انا مش فارقة عندي هل هو aerobic ولا + +146 +00:10:37,770 --> 00:10:41,630 +انا aerobic اللي هو من قدرة ايه؟ تستغله فإذا قلت + +147 +00:10:41,630 --> 00:10:46,030 +glucose utilization فبالفعل ال enterobacteriasi + +148 +00:10:46,030 --> 00:10:50,290 +بسطه و ال salomonas برضه بسطه لكن لو قلت + +149 +00:10:50,290 --> 00:10:55,300 +fermentationالإنتيرو بكتيرياسي positive بينما ال + +150 +00:10:55,300 --> 00:10:57,840 +pseudomonas يبدأ بدل مصطلحات يكون إيش اللي أقل + +151 +00:10:57,840 --> 00:11:00,880 +عنها معروفة تلتة إيش هم اللي أقل عنها تلتة؟ + +152 +00:11:00,880 --> 00:11:04,940 +Fermentative, Oxidative و Utilization كلمة + +153 +00:11:04,940 --> 00:11:08,120 +Utilization فينك مرة مش فارقة معناها إنه هيك ولا + +154 +00:11:08,120 --> 00:11:13,560 +هيك في كمان احتبار راح بعملش بنكبر محنا كل شوية كل + +155 +00:11:13,560 --> 00:11:19,000 +خمس دقايق بنكبر خمس دقايق الآن عندي بكتير اللي + +156 +00:11:19,000 --> 00:11:22,540 +عندها القدرة إنها تعمل oxidationولا fermentation + +157 +00:11:22,540 --> 00:11:26,560 +بيسموها Asacarolithica Asacarolithica يبقى + +158 +00:11:26,560 --> 00:11:29,600 +Utilization, Oxidation, Fermentation و + +159 +00:11:29,600 --> 00:11:34,360 +Asacarolithicus يعني غير قادرة على استغلال زي آخر + +160 +00:11:34,360 --> 00:11:37,840 +واحدة على الصبورة عنها اسمها Alkalogenes fecalis + +161 +00:11:37,840 --> 00:11:41,300 +neither ferments nor oxidizes الجلوكوز اللي هو + +162 +00:11:41,300 --> 00:11:43,940 +بتخمر ولا بتاكسد اللي هو الجلوكوز بينما + +163 +00:11:43,940 --> 00:11:47,540 +السودومولاس كريجنوزا اللي جابلها it oxidizes but + +164 +00:11:47,540 --> 00:11:48,440 +does not ferment + +165 +00:11:54,010 --> 00:11:57,790 +مش محطة محترمة مش carbon neutralizer كاربوهايدريت + +166 +00:11:57,790 --> 00:12:01,910 +لأنه .. لأنه .. لأنه بدأ مصدر كربوني و هتحتاري + +167 +00:12:01,910 --> 00:12:06,150 +المصدر الكربوني من مصادر أخرى مثلا من ال proteins + +168 +00:12:06,150 --> 00:12:11,830 +أو من ال .. ال lipids أو من ال nucleic acids فاهم؟ + +169 +00:12:11,830 --> 00:12:15,770 +يبقى متفقين الآن على أربع مصطلحات argue أنه الأمور + +170 +00:12:15,770 --> 00:12:19,150 +تكون واضحة بنفسنا دخلين في توضيح الأمور خلينا نوضح + +171 +00:12:19,150 --> 00:12:23,880 +كمان أمر تانيالان لما انا بقول glucose positive او + +172 +00:12:23,880 --> 00:12:26,400 +glucose negative او اي صفة من الصفات اللي درستوها + +173 +00:12:26,400 --> 00:12:28,600 +في المعمل من ال indole من ال methyl red من الفوكرس + +174 +00:12:28,600 --> 00:12:32,180 +بروسكاوه انتوا بتدرسوكوا ايها absolute بيقولك ال E + +175 +00:12:32,180 --> 00:12:34,660 +coli positive positive negative negative ال + +176 +00:12:34,660 --> 00:12:37,040 +enterobacter negative negative positive positive + +177 +00:12:37,040 --> 00:12:40,360 +بصبوط لل invic ده ال indole من ال methyl red من + +178 +00:12:40,360 --> 00:12:42,400 +الفوكرس بروسكاوه او ال citrate انا الان بده + +179 +00:12:42,400 --> 00:12:47,000 +افهمكوا مفهوم تاني مفهوم تشكيكي خالص تمام؟ مش + +180 +00:12:47,000 --> 00:12:51,840 +هنوظفه الآن لكن غصب عنه احنا هحتاجهلو جيبنا 100 + +181 +00:12:51,840 --> 00:12:55,380 +pseudomonas aeruginosa مائة species معرفين علي + +182 +00:12:55,380 --> 00:12:58,480 +انهم pseudomonas aeruginosa وسألناهم سؤال هل انتي + +183 +00:12:58,480 --> 00:13:04,540 +اكسيدايز بوستف المية اللي جينا منهم 97 بوستف و 3% + +184 +00:13:04,540 --> 00:13:08,640 +منهم negative تلاتة من المية negative ايه أوصف + +185 +00:13:08,640 --> 00:13:12,850 +الصفة؟بتقول ال post-it؟ إلكوا بقول post-it طبعا؟ + +186 +00:13:12,850 --> 00:13:15,750 +طب ده ال computer مش فيه إمكانيه إنه أنا أتعامل + +187 +00:13:15,750 --> 00:13:17,930 +معه أنا مش حاجة ده انا اصلا انا .. انا ملاقى + +188 +00:13:17,930 --> 00:13:21,450 +الصعوبة أحفظ ال post-it و ال negative صح؟ فكمان + +189 +00:13:21,450 --> 00:13:23,550 +أحفظ ال post-it و ال negative و جدّين ال + +190 +00:13:23,550 --> 00:13:26,170 +percentage ده بتقول إن ال post-it أو ال negative + +191 +00:13:26,170 --> 00:13:30,740 +هذا هيكون أمر صعب جدالا يقيقوا إلا قواعد البيانات + +192 +00:13:30,740 --> 00:13:34,240 +طبعا الحصول، لذلك البنات اللي نزلوا البرنامج اللي + +193 +00:13:34,240 --> 00:13:39,020 +اسمه IDBAC لو طلّوا على المصففات، هيلجوا أسماء + +194 +00:13:39,020 --> 00:13:43,240 +البكتيريا و أسماء الفحوصاتو مش مكتوب positive و + +195 +00:13:43,240 --> 00:13:48,780 +negative مكتوب percentage 97% , 99% , 100% , 50% + +196 +00:13:48,780 --> 00:13:51,240 +يعني في بعض الصفات قد تصل الى fifty و هذه اللي .. + +197 +00:13:51,240 --> 00:13:54,840 +لما تصل الى fifty بتسميها variable يعني هو ممكن + +198 +00:13:54,840 --> 00:13:58,520 +positive و ممكن negative أقل من fifty بتميل إلى + +199 +00:13:58,520 --> 00:14:01,440 +هتكون negative أكتر من fifty بتتميل إلى نقول أنها + +200 +00:14:01,440 --> 00:14:06,240 +positive لكن في النهاية يعني كثير من الصفات مش + +201 +00:14:06,240 --> 00:14:08,240 +absolute مش one hundred percent هذا اللي بدنا + +202 +00:14:08,240 --> 00:14:12,990 +نوصلكوا إلى كافة كلمةطيب Sodomeras aeruginosa + +203 +00:14:12,990 --> 00:14:19,330 +الجميلة ذات الرائحة الذكية و في نفس الوقت سيئة + +204 +00:14:19,330 --> 00:14:24,210 +السمعة سيئة السمعة لإنه it causes hard to treat + +205 +00:14:24,210 --> 00:14:29,030 +infections بتسبب أمراض صعب الشفاء منها حتى + +206 +00:14:29,030 --> 00:14:34,650 +باستخدام ال antibiotics جميلة لإن ألوانها براقة و + +207 +00:14:34,650 --> 00:14:37,330 +دراستوها انتوا في ال pigments لما كنا سليبوا ال + +208 +00:14:37,330 --> 00:14:39,730 +pigments blue green pigments blue green و الاخر + +209 +00:14:39,730 --> 00:14:44,000 +حمره بعناو جميلة لإنه بتنتج زي ما شايفينها احنا في + +210 +00:14:44,000 --> 00:14:49,580 +احد الأماكن هيتها grape-like odor اللي هي رائحة + +211 +00:14:49,580 --> 00:14:53,440 +تشبه رائحة العنب او بعض الأحيان يعني انا لعشرين + +212 +00:14:53,440 --> 00:14:57,160 +سنة بشم سودا مونس ماعمر شمت رائحة العنب بشم رائحة + +213 +00:14:57,160 --> 00:15:03,340 +جميلة fruity odor بسمها fruity odor إذن لون حلو و + +214 +00:15:03,340 --> 00:15:08,540 +رائحة جميلة تمام إلا إنه بتسبب hard to treat .. + +215 +00:15:08,540 --> 00:15:13,140 +hard to treat infectionsو الحقيقة انه حتى قبل + +216 +00:15:13,140 --> 00:15:17,460 +اكتشاف ال antibiotic البكتيريا معروفة بطبيعيها + +217 +00:15:17,460 --> 00:15:22,520 +مقاومة مش اكتسبت مقاومة هي بطبيعيها يعني من أول ما + +218 +00:15:22,520 --> 00:15:24,620 +اكتشفنا ال antibiotic هما بنعرف ان ال pseudomorous + +219 +00:15:24,620 --> 00:15:27,540 +ايه؟ resistant لل antibiotic natural resistant + +220 +00:15:27,540 --> 00:15:31,940 +نسموه غير ال natural resistance عندها المقدرة to + +221 +00:15:31,940 --> 00:15:35,920 +acquire resistance كمان يعني انا قبل فترة عرض + +222 +00:15:35,920 --> 00:15:40,610 +presentation في احد المؤتمراتووصلت نسبة ال + +223 +00:15:40,610 --> 00:15:43,850 +resistance لبعض ال antipatetic hormones 100% بمعنى + +224 +00:15:43,850 --> 00:15:47,650 +أن ولا عزلة اللي عزلناهم كانت sensitive لل + +225 +00:15:47,650 --> 00:15:50,270 +antipatetic اللي أصل هي sensitive لها يعني معناها + +226 +00:15:50,270 --> 00:15:53,010 +عخر عندها natural resistance و عندها acquired + +227 +00:15:53,010 --> 00:15:57,730 +resistance طيب المعنى المروسة يعنيالجملة الأولى + +228 +00:15:57,730 --> 00:16:04,670 +disregard كلمة group multiple هي single فهنشيل + +229 +00:16:04,670 --> 00:16:08,250 +الحق يعني من ال presentation فقط هنخلي single هو + +230 +00:16:08,250 --> 00:16:12,090 +الرفلجلة هنشيل كلمة هو multiple gram negative بس + +231 +00:16:12,090 --> 00:16:15,510 +الله it is obligate erupts طبعا obligate يعني + +232 +00:16:15,510 --> 00:16:19,150 +strict يعني مابتقدرش تناميها لها وقت طبعا بقولها + +233 +00:16:19,150 --> 00:16:22,350 +most strange كأي صفة من الصفات اللي قولنا عنها مش + +234 +00:16:22,350 --> 00:16:27,530 +absoluteOxidized usually and catalysed positively + +235 +00:16:27,530 --> 00:16:29,850 +هاي حتى ال Oxidized الصفة اللي احنا ميزنا فيها صدر + +236 +00:16:29,850 --> 00:16:33,930 +موارث على جهة بيقولنا مش مش مش مش absolute مش + +237 +00:16:33,930 --> 00:16:38,170 +absolute البعض الصليحات منها بتكون negative Non + +238 +00:16:38,170 --> 00:16:41,550 +-fermentative chemoheterotrophic respiratory + +239 +00:16:41,550 --> 00:16:46,070 +metabolism minimum nutritional requirements بمعنى + +240 +00:16:46,070 --> 00:16:50,830 +انهتستطيع إنها تعيش على أقل القيادة و أنا ممكن + +241 +00:16:50,830 --> 00:16:54,090 +قلتلكوا مرة على ال bottled water أو ال .. ال + +242 +00:16:54,090 --> 00:16:57,970 +mineral water اللي بنشتريه، بنشربه، إيش فيه مواد + +243 +00:16:57,970 --> 00:17:00,370 +غذائية ده؟ ال mineral water؟ ولا إيش فيه مواد + +244 +00:17:00,370 --> 00:17:03,130 +غذائية؟ مواد .. مواد بسيطة جدا، بال .. بال nano، + +245 +00:17:03,130 --> 00:17:07,330 +بال microgram ممكن تكون، بال ppm بيجسوها، طبعا؟ + +246 +00:17:07,330 --> 00:17:11,650 +ومع ذلك البكتيريا عندها المقدرة to thrive and .. + +247 +00:17:11,650 --> 00:17:14,670 +thrive and grow .. even grow in .. in this mineral + +248 +00:17:14,670 --> 00:17:20,350 +يعني they requirevery low nutritional requirements + +249 +00:17:20,350 --> 00:17:25,850 +بتحكيلكوا عن صدوموناس حد يعني بتخليكوا ذاكرين + +250 +00:17:25,850 --> 00:17:33,770 +الصدوموناس كويس بتعرفوا في روسيا سيبيريا طول السنة + +251 +00:17:33,770 --> 00:17:37,010 +تقريبا تلج هم عايشين في تلاجة أصلا في الفريزر + +252 +00:17:37,010 --> 00:17:40,210 +عايشين هم مش حتى في تلاجة في الفريزر فتخيلوا عند + +253 +00:17:40,210 --> 00:17:43,290 +واحد عنده سيارة السيارة في مي بيحطوها في الراديات + +254 +00:17:43,290 --> 00:17:48,010 +لإنها تبرد المحرك فتجمل الميفبروح بيحطولها + +255 +00:17:48,010 --> 00:17:52,950 +antifreeze .. antifreeze يعني مواد مضادة للتجمد من + +256 +00:17:52,950 --> 00:17:55,170 +ضمن المواد اللي .. اللي .. اللي اكتشفوها حطوها .. + +257 +00:17:55,170 --> 00:17:57,690 +حطولها Sodomonas Reginosa او من الأحلى Sodomonas + +258 +00:17:57,690 --> 00:18:01,350 +Sodomonas Reginosa ف Sodomonas لما حاطينها مع ال + +259 +00:18:01,350 --> 00:18:05,650 +.. مع الميه بطلت تتجمد طيب هذه نقطة تذكرها كويسة + +260 +00:18:05,650 --> 00:18:09,050 +انه منعانة التجمد في أحد السنوات أحد الدول اللي + +261 +00:18:09,050 --> 00:18:12,450 +صدفت الألعاب الأولومبية الشتوية اللي فيها تزولوا + +262 +00:18:12,450 --> 00:18:16,150 +العجليات أساسي المفروض ان دولة يكون مش .. مش .. مش + +263 +00:18:16,150 --> 00:18:19,680 +سعودية أكيدأنا صوت اللى بيكون الدولة يكون فيها + +264 +00:18:19,680 --> 00:18:25,380 +تلج، سنة الاستضافة صارنا المشكلة الثلج ما تحول + +265 +00:18:25,380 --> 00:18:29,960 +لجليد وبالتالي هتفشل ألعاب دول أمبريكية، اتصلوا في + +266 +00:18:29,960 --> 00:18:32,420 +ال biotechnology groups، جالنا في بيتنا، جالنا نحط + +267 +00:18:32,420 --> 00:18:37,060 +صدومورس، فخايل الأول مرة مانعت .. المرة حاطينة + +268 +00:18:37,060 --> 00:18:41,500 +عاملة .. حوّلت التلج إلى جليدلما انا بدي عزل ال + +269 +00:18:41,500 --> 00:18:47,260 +micro غريب الصفات بمعنى بيأكل او بيكسر مركبات + +270 +00:18:47,260 --> 00:18:51,460 +غريبة لما غالبا بدل العالمين على ال Sodamores + +271 +00:18:51,460 --> 00:18:57,660 +عندها wide range of metabolismيعني خلّينا نقول + +272 +00:18:57,660 --> 00:19:03,120 +مصنع .. مصنع لل enzymes مصنع كبير لل enzymes عندها + +273 +00:19:03,120 --> 00:19:07,060 +مقدرة مهولة على استغلال الأشياء بتخليش إشي اللي لا + +274 +00:19:07,060 --> 00:19:09,600 +تقدر تستغل و بالتالي pseudomonas بالفعل كان + +275 +00:19:09,600 --> 00:19:12,220 +survive for long الشيء السيء اللي بيقوله عن + +276 +00:19:12,220 --> 00:19:15,260 +pseudomonas احنا كنا زمان أنه لما نروح المستشفيات + +277 +00:19:15,260 --> 00:19:18,200 +عشان نرفع من ملويات مريضنا ناخدله bouquet ورد، + +278 +00:19:18,200 --> 00:19:25,570 +مظبوط؟ جميل، حلو، لكن من الأقل طالعلأنه هي normal + +279 +00:19:25,570 --> 00:19:30,510 +.. normal microbiota على ال plants فواقعي اعمل .. + +280 +00:19:30,510 --> 00:19:32,930 +لو بناخدله ورد احنا المريض نوديله هنا to the + +281 +00:19:32,930 --> 00:19:37,450 +monocery خاصة لو يكون مريض حروق او يكون مريض جروح + +282 +00:19:37,450 --> 00:19:41,130 +يعني عمليات او كده هذا بتأثر جدا بال .. و طبعا هي + +283 +00:19:41,130 --> 00:19:44,770 +بتعمل opportunistic infection اكتر ناس بيداجوا من + +284 +00:19:44,770 --> 00:19:48,170 +البكتيريا هم اقسام الحروق و اقسام العمليات + +285 +00:19:48,170 --> 00:19:50,570 +الجروحية لان بتعمل wound and burn + +286 +00:19:54,900 --> 00:19:58,920 +بكون إيش هو القاتل للمريض بقدر ما بتكون ال burn + +287 +00:19:58,920 --> 00:20:02,320 +infection هي المسئولة عن .. و طبعا ال burn + +288 +00:20:02,320 --> 00:20:04,920 +infection number one cause لها pseudomonas + +289 +00:20:04,920 --> 00:20:07,940 +erogenous و عملنا دراسة و نشرتها السنة الماضية أو + +290 +00:20:07,940 --> 00:20:10,860 +اللي قبلها مش فاكر بالضبط .. نشرتها في جورنال + +291 +00:20:10,860 --> 00:20:14,280 +أجنبي بيتكلم على ان number one cause of burn + +292 +00:20:14,280 --> 00:20:16,640 +infection في مستشفى الشيفة كان هو pseudomonas + +293 +00:20:16,640 --> 00:20:20,500 +erogenous و لجناها في دين المرضى و لجناها على فضل + +294 +00:20:20,500 --> 00:20:23,320 +دين ال health care workers و على الأسطح في كل مكان + +295 +00:20:23,320 --> 00:20:26,010 +في المستشفىفي عيادة اللي هو لجيناها كمان Sodium + +296 +00:20:26,010 --> 00:20:30,950 +monoxidane مثلا فقيت الشيء في الموضوع أنه ال + +297 +00:20:30,950 --> 00:20:34,150 +minimal nutritional requirements بتخليها to + +298 +00:20:34,150 --> 00:20:37,390 +survive in various environments in harsh + +299 +00:20:37,390 --> 00:20:41,270 +conditions مش زي البكيريا اللي لو تركناها دي اكتين + +300 +00:20:41,270 --> 00:20:45,150 +برا بتزعل و تموت يعني لأ هذه عندها المقدرة على + +301 +00:20:45,150 --> 00:20:50,710 +أنها تعيش في البيئة بشكل كبيرmany organic + +302 +00:20:50,710 --> 00:20:53,310 +compounds used as carbon and nitrogen sources, but + +303 +00:20:53,310 --> 00:20:56,690 +only few carbohydrates by oxidative metabolism، + +304 +00:20:56,690 --> 00:20:59,710 +الجلوكوز used oxidatively، هي وسط الجلوكوز من + +305 +00:20:59,710 --> 00:21:02,350 +ناحية اللي هو ال Oxidation، هذا النقطة اللي بدنا + +306 +00:21:02,350 --> 00:21:06,230 +نركز عليها كمان، lactose، negative، on مكونكي + +307 +00:21:06,230 --> 00:21:11,250 +أجانب، lactose negative يعني non-lactose fermenter + +308 +00:21:11,630 --> 00:21:16,290 +يعني شكل المقنكي هيكون زي شكل السلمونيلا زي شكل + +309 +00:21:16,290 --> 00:21:20,830 +الشجلة زي شكل البروتياز زي اليرسينيا هدولة من وين + +310 +00:21:20,830 --> 00:21:25,250 +هدولة من الاسماء من ال enterobacteriasis يبقى ال + +311 +00:21:25,250 --> 00:21:27,750 +Sodomonas و ال enterobacteriasis النالكتوزفرمنتر + +312 +00:21:27,750 --> 00:21:33,110 +سيبدو كأنهم شكل نوع واحد يعني النالكتوزفرمنتر + +313 +00:21:33,110 --> 00:21:35,910 +فبنعرف أن مش كل إشي بينمو المقنكي هو + +314 +00:21:35,910 --> 00:21:40,980 +enterobacteriasis قد يكون Sodomonas و أخواتهاومنها + +315 +00:21:40,980 --> 00:21:44,340 +الاسينيتوباكتر اللي هو non-lactose fermenter بس + +316 +00:21:44,340 --> 00:21:47,720 +الفرق بين ال enterobacteres اللي ذكرتهم و بين ال + +317 +00:21:47,720 --> 00:21:51,620 +pseudomonas ال triple sugar iron agar ايش هيطلع ال + +318 +00:21:51,620 --> 00:21:56,380 +triple sugar iron agar تبع ال pseudomonas؟ + +319 +00:21:56,380 --> 00:21:58,600 +triple sugar iron agar تبع ال pseudomonas ايش + +320 +00:21:58,600 --> 00:22:04,560 +هيطلع؟ + +321 +00:22:04,560 --> 00:22:07,660 +ايش + +322 +00:22:07,660 --> 00:22:11,280 +هيطلع النتائج النهائية المهمة؟اللون طبعا هو مش + +323 +00:22:11,280 --> 00:22:16,920 +هيكون أخضر، + +324 +00:22:16,920 --> 00:22:24,260 +هو مش مش من اللوحة ده، هيطلع + +325 +00:22:24,260 --> 00:22:28,350 +أحمر على أحمرلأن لو هتعمل fermentation للجليكوز و + +326 +00:22:28,350 --> 00:22:31,810 +لو هتعمل fermentation للاكتوز ولا للساكروزا + +327 +00:22:31,810 --> 00:22:35,610 +وبالتالي مش هيتغير اللون فهي هتكون K على K زي ما + +328 +00:22:35,610 --> 00:22:40,850 +هي عمليها هتتغير بينما لو كانت سالمونيلا هتكون K + +329 +00:22:40,850 --> 00:22:44,150 +على A لو كانت البروتيز هتكون K على A لو كانت + +330 +00:22:44,150 --> 00:22:47,010 +الشيلة هتكون K على A لأن كل ال انترو بكتيرياز + +331 +00:22:47,010 --> 00:22:50,950 +الجليكوز فرمنتها بينما اللي ذكرناه دول نار لاكتوز + +332 +00:22:50,950 --> 00:22:51,410 +فرمنتها + +333 +00:22:54,760 --> 00:22:58,480 +طيب some strains produces pigment او diffusable و + +334 +00:22:58,480 --> 00:23:02,300 +هي soluble pigment زى مثلا اللى هو ال biocyanine و + +335 +00:23:02,300 --> 00:23:04,640 +هذا هو ال blue pigment و ال fluorescein اللى هو ال + +336 +00:23:04,640 --> 00:23:07,040 +yellow pigment اذا two pigments معروفين ل ال + +337 +00:23:07,040 --> 00:23:10,800 +pseudomonas اللى هم ال biocyanine و هي زرقاء و ال + +338 +00:23:10,800 --> 00:23:15,000 +fluorescein بتكون yellowسيد موراس إرجنوزا يقول + +339 +00:23:15,000 --> 00:23:18,300 +إنها produces grape-like odor أو اللي سميناها + +340 +00:23:18,300 --> 00:23:22,680 +fruity odor and blue-green pus and cornice. إيش عن + +341 +00:23:22,680 --> 00:23:26,740 +ال blue-green pus؟ الصديد اللي يمكن يهانتش من هذه + +342 +00:23:26,740 --> 00:23:30,940 +البكتيريا برضه بيكون ماخد لون اللي هو البكتيريا + +343 +00:23:32,060 --> 00:23:35,960 +الدُولة الأخيرة طبعاً أهميتها أكد عليها في بيت + +344 +00:23:35,960 --> 00:23:39,460 +المحاضرة أنه broad spectrum .. broad antibiotic + +345 +00:23:39,460 --> 00:23:43,860 +resistance و بنوصفها بإنها MDR أو multiple drug + +346 +00:23:43,860 --> 00:23:48,480 +resistance شوفوا + +347 +00:23:48,480 --> 00:23:51,720 +عليك، في عندي قائمة من الأمراض الآن اللي موجودة + +348 +00:23:51,720 --> 00:23:55,200 +على الصبورة، صح؟اللي هو تحت أنواريش مكتوبين + +349 +00:23:55,200 --> 00:24:00,540 +opportunistic nosocomial infections يعني غالبا + +350 +00:24:00,540 --> 00:24:06,320 +hospital acquired و كمان مرة opportunistic هذه + +351 +00:24:06,320 --> 00:24:10,380 +ليست قائمة extensive هذه sample من الأمراض اللي + +352 +00:24:10,380 --> 00:24:13,060 +بتسببها ال pseudomonas aeruginosa + +353 +00:24:18,910 --> 00:24:21,970 +burn and wound infection قلنا لكوا انها number one + +354 +00:24:21,970 --> 00:24:24,530 +cause of burn and wound infection وبالتالي بدنا + +355 +00:24:24,530 --> 00:24:28,870 +نكون حذرين جدا وكل الإجراءات في قسم الحروب بتكون + +356 +00:24:28,870 --> 00:24:33,030 +منصبة على مدعى على مدعى وصول pseudomonas + +357 +00:24:33,030 --> 00:24:36,890 +aeruginosa إلى المرضى لكن شوفوا pseudomonas + +358 +00:24:36,890 --> 00:24:41,310 +aeruginosa كيف وصلت إلى المرضى بتعرفوا المواد + +359 +00:24:41,310 --> 00:24:45,050 +المطاهرة اللي لجوا فيها pseudomonas aeruginosa + +360 +00:24:46,080 --> 00:24:50,060 +resistant البكتيريا resistant البكتيريا resistant + +361 +00:24:50,060 --> 00:24:59,080 +البكتيريا + +362 +00:24:59,080 --> 00:25:05,100 +resistant البكتيريا + +363 +00:25:05,100 --> 00:25:10,280 +resistant البكتيريا + +364 +00:25:10,280 --> 00:25:12,620 +resistant البكتيريا resistant البكتيريا resistant + +365 +00:25:12,620 --> 00:25:12,620 +البكتيريا resistant البكتيريا resistant البكتيريا + +366 +00:25:12,620 --> 00:25:12,620 +resistant البكتيريا resistant البكتيريا resistant + +367 +00:25:12,620 --> 00:25:12,620 +البكتيريا resistant البكتيريا resistant البكتيريا + +368 +00:25:12,620 --> 00:25:12,620 +resistant البكتيريا resistant البكتيريا resistant + +369 +00:25:12,620 --> 00:25:12,620 +البكتيريا resistant البكتيريا resistant البكتيريا + +370 +00:25:12,620 --> 00:25:12,640 +resistant البكتيريا resistant البكتيريا resistant + +371 +00:25:12,640 --> 00:25:12,640 +البكتيريا resistant البكتيريا resistant البكتيريا + +372 +00:25:12,640 --> 00:25:12,640 +resistant البكتيريا resistant البكتيريا resistant + +373 +00:25:12,640 --> 00:25:12,640 +البكتيريا resistant البكتيريا resistant البكتيريا + +374 +00:25:12,640 --> 00:25:12,640 +resistant البكتيريا resistant البكتيريا resistant + +375 +00:25:12,640 --> 00:25:12,640 +البكتيريا resistant البكتيريا resistant البكتيريا + +376 +00:25:12,640 --> 00:25:12,640 +resistant البكتيريا resistant البكتيريا resistant + +377 +00:25:13,230 --> 00:25:18,890 +حمام ساخن عشان يحمموا المرضى عشان يشيلوا الجلد + +378 +00:25:18,890 --> 00:25:23,670 +الميت لإن الجلد الميت هذا .. الجلد الميت يعتبر + +379 +00:25:23,670 --> 00:25:27,410 +مادة غذائية للبكتيريا و إزالته أمر ضروري جدا + +380 +00:25:27,410 --> 00:25:31,810 +للعلاج و لمنع الاتهاد لما بحنا قاعد و أخدنا عينات + +381 +00:25:31,810 --> 00:25:35,690 +.. أخدنا من هذا ال hot tub اللي هو ال .. البانيو + +382 +00:25:35,690 --> 00:25:39,350 +تمام؟ و عزلنا تدورنا سريجيا مثلا .. مثلا تحكي أنه + +383 +00:25:39,350 --> 00:25:43,390 +مريض بيعطي كمالة ميملمريض اخر اذا كان ماكانش فيه + +384 +00:25:43,390 --> 00:25:46,970 +تنظيف و disinfection بشكل جيد لجينا صدموناس على + +385 +00:25:46,970 --> 00:25:51,490 +الصابون اللى بيستخدموه القطط بقى يبقى طيب لما يغسل + +386 +00:25:51,490 --> 00:25:56,630 +ايه بدل ما ينظف بياخد صدموناس رجل نفسه هذا كمان + +387 +00:25:56,630 --> 00:25:59,670 +مرة هذا الكلام لو كان في البيت مافيش مشكلة بس لما + +388 +00:25:59,670 --> 00:26:03,390 +يكون في مستشفى انتوا تذكروا انا بتكلم عن مين مريض + +389 +00:26:03,390 --> 00:26:09,210 +حروق يعني مالوش جلديبقى opportunistic infection + +390 +00:26:09,210 --> 00:26:13,190 +سهلة جدا .. سهلة جدا بينما Sodomones بالنسبة لإن + +391 +00:26:13,190 --> 00:26:16,090 +احنا ك healthy individuals غالبا مش هتعملنا أي + +392 +00:26:16,090 --> 00:26:21,210 +إشكالية إلا إذا اخترقت الجلد يعني عبر جرح أو عبر + +393 +00:26:21,210 --> 00:26:28,310 +.. طيب إذا لم تتم معالجتها ال barrel wound + +394 +00:26:28,310 --> 00:26:31,760 +infection غالبا بتقدي إلىبكتيريميا قد تصل إلى + +395 +00:26:31,760 --> 00:26:35,180 +نيومونيا و قد تصل إلى meningitis برضه من ال .. من + +396 +00:26:35,180 --> 00:26:39,420 +الأمراض المشهورة السيئة urinary tract infection في + +397 +00:26:39,420 --> 00:26:42,620 +البراج السباحة فيها اسمها swimmers ear بيخلص + +398 +00:26:42,620 --> 00:26:44,960 +السباحة بعد ساعتين تلاتة بيصير الدينه حمره + +399 +00:26:44,960 --> 00:26:49,080 +external ال external ear بيسموه swimmers ear من + +400 +00:26:49,080 --> 00:26:51,780 +اسم ال .. اللي هي أذن السباح يعني اللي بيسبحوا في + +401 +00:26:51,780 --> 00:26:56,350 +البراج السباحةالأذن الخارجية الـ otitis media + +402 +00:26:56,350 --> 00:27:00,010 +إلتهاب الأذن الداخلية الأذن الداخلية several skin + +403 +00:27:00,010 --> 00:27:04,630 +infections كمان برضه ممكن تعمل إلتهابات في الجلد + +404 +00:27:04,630 --> 00:27:06,170 +بأشكال متنوعة + +405 +00:27:09,430 --> 00:27:13,770 +الامراض لما احنا قولنا pseudomonas and non + +406 +00:27:13,770 --> 00:27:17,090 +fermentants يبقى pseudomonas هي الأشهر في المجموعة + +407 +00:27:17,090 --> 00:27:20,630 +خدوا بالك كل الميكروبات اللي ذكرناها في المجموعة + +408 +00:27:20,630 --> 00:27:24,810 +تقريبا بتسبب نفس الأمراض لكن بدرجة من أقل انتشارا + +409 +00:27:24,810 --> 00:27:27,510 +من pseudomonas erogenous إلى ان pseudomonas + +410 +00:27:27,510 --> 00:27:29,550 +erogenous في الوقت الحالي هي المسيطرة هي قائدة + +411 +00:27:29,550 --> 00:27:33,090 +المجموعة اللي احنا اتكلمنا عنها الدكتور انت حقيط + +412 +00:27:33,090 --> 00:27:35,630 +ان عزلها الصبونات في المجموعات + +413 +00:27:38,610 --> 00:27:42,190 +لأ هو الحل انه اول حاجة الصابون ال manufacturing + +414 +00:27:42,190 --> 00:27:46,170 +تبقى عقصية و لما احنا .. لما وجدنا بالفعل في + +415 +00:27:46,170 --> 00:27:50,070 +الصابون و كان من الفضل اللي بيرجع لطلبات من قسم + +416 +00:27:50,070 --> 00:27:55,130 +العلم التطبيقى المخبرية قاموا دراسة لفصفة الماضي و + +417 +00:27:55,130 --> 00:27:59,310 +النتائج الدراسة تم عرضها على المسؤولين في وزارة + +418 +00:27:59,310 --> 00:28:02,970 +الصحةوعلى مختبر الصحة العامة وعلى اللجان ال + +419 +00:28:02,970 --> 00:28:07,910 +infection control و أصبح هناك إجراء روتيني بتم أن + +420 +00:28:07,910 --> 00:28:13,110 +أي صوابين تدخل المستشفى يبقى يتم فحصها ويعدي + +421 +00:28:13,110 --> 00:28:15,450 +البكتيرى اللي فيها و التعرف على بعض القصاص اللي + +422 +00:28:15,450 --> 00:28:18,810 +فيها قبل إدخال الخدمة فكانت الدراسة الصحية لها + +423 +00:28:18,810 --> 00:28:23,440 +قيمة جيدة ونتج عنها actions يعنيالان الموضوع ال .. + +424 +00:28:23,440 --> 00:28:27,280 +ال .. ال .. ال infection control في المستشفيات، + +425 +00:28:27,280 --> 00:28:29,960 +هنا الصيحة عندنا لجان اسمها infection control + +426 +00:28:29,960 --> 00:28:33,320 +committees لكن مدى فعليتها أنا بحط عليها + +427 +00:28:33,320 --> 00:28:37,520 +بالاستفادة، هي إليها صيحة يمكن من ال ألف وتسعمية و + +428 +00:28:37,520 --> 00:28:41,300 +أربعة و تسعين و خمسة و تسعين من القرن الماضي، لكن + +429 +00:28:41,300 --> 00:28:45,850 +بتنشط سنةو بتقعد سنتين، تلاتة، مهمة لو أربعة و + +430 +00:28:45,850 --> 00:28:49,130 +بعدين بيجي واحد تاني بنشطها سنة و بتقعد .. مافيش + +431 +00:28:49,130 --> 00:28:52,430 +consistency فيه لذلك مالاجيش لا إجراءات مكتوبة + +432 +00:28:52,430 --> 00:28:55,610 +الطبيب اللي بيجي جديد مثلا أو الممرض اللي بيشتغل + +433 +00:28:55,610 --> 00:28:58,110 +جديد بشوف الناس بيشتغل بطريقة معينة تدخل في ال + +434 +00:28:58,110 --> 00:29:02,990 +system .. فغالبا مافيش عندنا system جيد الصيدل + +435 +00:29:02,990 --> 00:29:06,520 +infection control هذا بتطلب بالفعل أنهيكون فيه + +436 +00:29:06,520 --> 00:29:10,580 +قرار جريء وفيه action جريء بإنه يجب أن يكون جزء من + +437 +00:29:10,580 --> 00:29:13,860 +هيكرية المستشفى ان يكون فيه infection control + +438 +00:29:13,860 --> 00:29:17,660 +committee فعالة و تراقب و تحاسب على لو صار في out + +439 +00:29:17,660 --> 00:29:21,020 +break لقدر الله في المستشفى ان تحاسب على تقصيرها + +440 +00:29:21,020 --> 00:29:25,000 +في هذا المشكلة ال microbe التاني في السلسلة + +441 +00:29:25,000 --> 00:29:28,540 +الواقعية الاسينتوفاكتر بوماني هذا بصلاه ولا كوكب؟ + +442 +00:29:35,910 --> 00:29:39,530 +هذه بتضحك عليكوا، هذه بتقولكوا أنا بس ال like لكن + +443 +00:29:39,530 --> 00:29:43,810 +ممكن تاخد شكل ال cooksai مين أخد cooksai gram + +444 +00:29:43,810 --> 00:29:49,410 +negative cooksai درسناها gram negative cooksai + +445 +00:29:52,980 --> 00:29:58,300 +النيسيريا .. النيسيريا هي gram-negative-diplokai، + +446 +00:29:58,300 --> 00:30:02,960 +الأسينيتوفاكتر mimics نيسيريا، كلمة mimics يعني + +447 +00:30:02,960 --> 00:30:08,000 +تحاكي، وكانوا زمان يسموها ميميا، ميميا من mimic، + +448 +00:30:08,000 --> 00:30:10,900 +it mimics the appearance of نيسيريا جنون + +449 +00:30:13,340 --> 00:30:16,700 +طيب اللي .. اللي .. اللي بتحبوش تحفظوه و بتحبوا + +450 +00:30:16,700 --> 00:30:21,200 +تفهموه كيف بدي اميز النيسيريا عن الأسينيتوباكتر + +451 +00:30:21,200 --> 00:30:24,500 +التنتين تحت الميكروسكوب وظهرولي انهم إجرام + +452 +00:30:24,500 --> 00:30:29,840 +نيجادردبلوكوركايف؟ كيف بدي اميزهم عن بعض؟ حكينا عن + +453 +00:30:29,840 --> 00:30:33,320 +الفيلم انت .. عشان انه الأسين .. الأسينيتوباكتر .. + +454 +00:30:33,320 --> 00:30:36,260 +ايه دخل الفيلم؟ ايه دخل؟ ال .. ال .. ال .. ال .. + +455 +00:30:36,260 --> 00:30:39,900 +النيسيريا .. النيسيريا أيضا aerobic النيسيريا + +456 +00:30:39,900 --> 00:30:42,240 +aerobic يعني بتاعة من ال oxidative بتاعة من ال + +457 +00:30:54,170 --> 00:31:00,510 +عن صفة اذكركوا ان الاسينيتوباكتر اكسيدايز نيجاتيف + +458 +00:31:00,510 --> 00:31:06,050 +بينما ان يسيريا احد اهم صفاتها و لو رجعتوا + +459 +00:31:06,050 --> 00:31:09,110 +للمحاضرة تبعتي هتلاقوا ان انا ركزت على كلمة + +460 +00:31:09,110 --> 00:31:12,530 +اكسيدايز positive على ان صفتها اكسيدايز positive + +461 +00:31:12,530 --> 00:31:15,510 +فإذا في يوم من الأيام انا عزلت بكتيريا + +462 +00:31:19,640 --> 00:31:23,520 +هو الـ Oxidase إذا Oxidase positive فهي نيسيريا + +463 +00:31:23,520 --> 00:31:27,080 +إذا Oxidase negative فهي أسينيتوبكتر يعني دي كتير + +464 +00:31:27,080 --> 00:31:32,640 +نقطة مهمة كمان مرة هي gram negative بس ال life لكن + +465 +00:31:32,640 --> 00:31:36,080 +it can mimic the appearance of gram negative + +466 +00:31:36,080 --> 00:31:42,460 +دبلوكوكتر طيب أسينيتوبكتر بوماني في عنا clinical + +467 +00:31:42,460 --> 00:31:45,440 +syndrome opportunistic infection أنا قلتلكوا قبل + +468 +00:31:45,440 --> 00:31:49,790 +شويه لسه مقدم قلتلكوا كل ال .. ال .. ال ..زميلات + +469 +00:31:49,790 --> 00:31:52,810 +.. زميلات Sodomonas كلهم بيسببوا تقريبا نفس + +470 +00:31:52,810 --> 00:31:57,690 +الأمراض لكن بدرجة أقل لكن هذا ال microbe لما احنا + +471 +00:31:57,690 --> 00:32:04,030 +درسناه طلاب زي حضراتكم بالفعل مر علينا كلمة واحدة + +472 +00:32:04,030 --> 00:32:07,690 +Acinetobacter تشبه Sodomonas لكن اكسيدايز نيجاتيب + +473 +00:32:07,690 --> 00:32:10,450 +وهي .. هي .. هي اللي درسناها عن ال .. ال .. ال + +474 +00:32:10,450 --> 00:32:13,760 +Acinetobacterاليوم الأسنة tobacco تحتلت عناوين + +475 +00:32:13,760 --> 00:32:18,460 +الصحف والمجلات والأخبار وصارت شهرتها زي شهر ال + +476 +00:32:18,460 --> 00:32:21,060 +pseudomonas و أكتر من ال pseudomonas طبعا عارفين + +477 +00:32:21,060 --> 00:32:26,560 +إيش اللي بيخلل بكتير تان شهر؟ إما الأمراض أو + +478 +00:32:26,560 --> 00:32:29,900 +صعوبتها يعني خدوا بالكوا بعض الأحيان واحد بيعمل + +479 +00:32:29,900 --> 00:32:34,960 +عمل واحد تمام؟ بيجلب الدنيا كلها وعمل واحد وفي ناس + +480 +00:32:34,960 --> 00:32:39,040 +بتعمل مليون عمل تجلب الدنيا فهذه تعمل عمل اللي هو + +481 +00:32:39,040 --> 00:32:43,850 +ال diseaseصار أصبح .. هدى بيسموها super bug .. + +482 +00:32:43,850 --> 00:32:46,790 +لبسله super bug .. سنوموله super bug .. ال .. ال + +483 +00:32:46,790 --> 00:32:47,030 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +484 +00:32:47,030 --> 00:32:47,530 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +485 +00:32:47,530 --> 00:32:49,030 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +486 +00:32:49,030 --> 00:32:49,590 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +487 +00:32:49,590 --> 00:32:50,090 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +488 +00:32:50,090 --> 00:32:50,110 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +489 +00:32:50,110 --> 00:32:52,750 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +490 +00:32:52,750 --> 00:32:54,070 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +491 +00:32:54,070 --> 00:32:56,310 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +492 +00:32:56,310 --> 00:32:56,310 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +493 +00:32:56,310 --> 00:33:04,210 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +494 +00:33:04,210 --> 00:33:08,550 +ال .. ال .. + +495 +00:33:11,230 --> 00:33:14,910 +من أول جرح أو حرق، حرق، بعدين من الدم، نفس + +496 +00:33:14,910 --> 00:33:18,450 +البكتيريا أسينيدوباكتر و resistant للكل و بعد فترة + +497 +00:33:18,450 --> 00:33:22,590 +واجيزة عضلت من ال CSF نفس المريض و نفس الشيء و بعد + +498 +00:33:22,590 --> 00:33:25,930 +فترة لم يقعد هناك مريض ناخد منه عينة، توفي المرض + +499 +00:33:27,260 --> 00:33:31,620 +لأنه أصيب بميكروب بسيط زي الacinetobacter اللي هو + +500 +00:33:31,620 --> 00:33:35,120 +الان super bug اللي هو multiple drug resistant و + +501 +00:33:35,120 --> 00:33:39,680 +غير قادر على أنه نتعامل معاه وهذا كله من ال abuse + +502 +00:33:39,680 --> 00:33:47,110 +لسوق الاستخدام و ال misuse لل antibioticsو توظيف + +503 +00:33:47,110 --> 00:33:49,730 +ال antibiotics يعني انا بقولكوا يعني معظم الناس + +504 +00:33:49,730 --> 00:33:54,690 +بيستخدم ال antibiotic بدون وصفة طبية و كلمة بدون + +505 +00:33:54,690 --> 00:33:57,390 +وصفة طبية انه يختار ال antibiotic و طبعا لو راح + +506 +00:33:57,390 --> 00:33:59,590 +الصيدليب يقدر يقطر الصيدليب برضه ال antibiotic على + +507 +00:33:59,590 --> 00:34:03,230 +.. على .. على يعني على هواه .. على هواه يمكن يكون + +508 +00:34:03,230 --> 00:34:07,430 +للتسويق فقط لغير مش ضرور يكون يعني انه والله فعال + +509 +00:34:07,880 --> 00:34:10,740 +ويمكن المرض مابحتاجش أساساً antibiotic زي ال viral + +510 +00:34:10,740 --> 00:34:13,380 +infections اللي معظمنا بيوصاب فيها ويقولوا انه + +511 +00:34:13,380 --> 00:34:16,540 +ماخد antibiotic مش هيرتاح نفسيه يعني بلاسفش هفتر + +512 +00:34:16,540 --> 00:34:22,140 +فللأسف هذا فيانة misuse واللي بيشجع عليه عدم وجود + +513 +00:34:22,140 --> 00:34:25,900 +نظام يمنع صرف الأدوية اللي زي ال antibiotic بدون + +514 +00:34:25,900 --> 00:34:30,570 +وصفة طبيةواللي بيزيد المشكلة أكتر عدم ولود ثقافة + +515 +00:34:30,570 --> 00:34:34,150 +بين الأطباء اللي هم الأصل الحمات في هذا الموضوع + +516 +00:34:34,150 --> 00:34:38,870 +عدم ولود الثقافة هذه أساساً فبيستمع .. بدل ما + +517 +00:34:38,870 --> 00:34:41,610 +يستمع لل science في ال .. في ال .. في ال + +518 +00:34:41,610 --> 00:34:45,030 +antibiotics بيستمع لمندبي المبيعات في الشركات + +519 +00:34:45,030 --> 00:34:48,090 +الأدوية اللي بيجيبوا يقولوا ال amoxicillin is + +520 +00:34:48,090 --> 00:34:50,850 +effective against كده و كده و كده .. هذا كلام في + +521 +00:34:50,850 --> 00:34:54,860 +الستنات في القرن الماضيالكلام هو صحيح، بس اليوم ال + +522 +00:34:54,860 --> 00:34:58,620 +amoxicillin مش effective لل .. للسفن الكوكاس اوريا + +523 +00:35:00,110 --> 00:35:02,910 +فانت لما انت توصف ال .. ال .. ال .. اموكسيلين + +524 +00:35:02,910 --> 00:35:06,610 +لمريد عنده resistant .. ال .. ال .. ال .. stuff + +525 +00:35:06,610 --> 00:35:09,350 +اللي بوكاس اورت resistant انت بتساهم في قتل ال + +526 +00:35:09,350 --> 00:35:13,110 +sensitive و بتنمي مين ال resistance فللأسف يعني + +527 +00:35:13,110 --> 00:35:17,030 +هذه الظاهرة موجودة بكثرة في قطاع غزة وظاهرة ملهوظة + +528 +00:35:17,030 --> 00:35:19,830 +يعني نرجع ان ان شاء الله حد ينتبه من المسؤولين على + +529 +00:35:19,830 --> 00:35:23,640 +حوالي قدر السطوع الصحيح يعني انا خطبتووزير الصحة + +530 +00:35:23,640 --> 00:35:27,380 +كتاب كامل ابعتله خطط عمل كيف يستغلوا البيانات + +531 +00:35:27,380 --> 00:35:31,520 +الموجودة عندهم بحيث انه على الأقل يصدر تقرير كل 6 + +532 +00:35:31,520 --> 00:35:35,560 +أشهر يعطي صورة واضحة عن مدى ال resistance في ال + +533 +00:35:35,560 --> 00:35:39,200 +antibiotics، بيطلع تقارير لكن متقطع، مش دايما احنا + +534 +00:35:39,200 --> 00:35:45,120 +كنا متقططينمافيش أي إجراءات اتقدت من ذاك الوقت إلى + +535 +00:35:45,120 --> 00:35:49,580 +اليوم لتحسين من صرف ال antibiotics وترشيد + +536 +00:35:49,580 --> 00:35:53,260 +استهلاكها وتنظيم عملها للأسف مافيش قوانين طلعت + +537 +00:35:53,260 --> 00:35:56,420 +بهذا المشكلة + +538 +00:36:01,430 --> 00:36:05,490 +Bones and septicemia الإبديمولوجي كلمة niches اللي + +539 +00:36:05,490 --> 00:36:10,610 +هي يعني الأماكن الصغيرة نوصف فيها مثلا بيقول الفم + +540 +00:36:10,610 --> 00:36:16,170 +احنا الفم بيئة واحدة ولا بيئات بيئات حاجة اتفقنا + +541 +00:36:16,170 --> 00:36:21,850 +كل بيئة في الفم بيسميها niche فوين الخروم؟ خروم + +542 +00:36:21,850 --> 00:36:26,670 +بيئي بالأحرى natural environments في الطبيعة + +543 +00:36:26,670 --> 00:36:30,530 +الموجودة في الميه في النباتات moist surfaces in + +544 +00:36:30,530 --> 00:36:35,200 +hospitalsزي مثلا Respiratory Therapy و الناس + +545 +00:36:35,200 --> 00:36:40,960 +بتعرفوا اللي محطها مثلا على الهادره في بعض الأجهزة + +546 +00:36:40,960 --> 00:36:43,320 +اللي .. اللي .. اللي بيحطوها في ال .. ما بتشوفوهاش + +547 +00:36:43,320 --> 00:36:45,840 +انتوا هي اللي بيحطوها في ال intensive care unit + +548 +00:36:45,840 --> 00:36:50,000 +اللي بتبقى .. اللي بتوفر pressure معين في ال lungs + +549 +00:36:50,000 --> 00:36:55,190 +فالمريض ماتنفسش من .. من نفسه هوطبعا يتولد لغوى + +550 +00:36:55,190 --> 00:36:59,490 +اصبعا عندها دي برضه كمان بيكون موجود فيها نوع من + +551 +00:36:59,490 --> 00:37:03,110 +الترطيب عشان ماتجفش ال lungs فبرضه البكتيريا + +552 +00:37:03,110 --> 00:37:06,930 +الاسينتروبكتر ممكن تأنه فيه لذلك بيلاجي في + +553 +00:37:06,930 --> 00:37:10,330 +المستشفيات اكتر استخدام لل antibiotic لانه بنعمل + +554 +00:37:10,330 --> 00:37:15,470 +prophlaxis بنمنع بكتيريا تقتل كل مزارة إجراءاتنا + +555 +00:37:15,470 --> 00:37:19,410 +اللي بنسميها invasive proceduresكل ميلاد إمكانية + +556 +00:37:19,410 --> 00:37:24,790 +إنه ننقل ميكروبات للمرضى أعطيكم مثال صغير baby طفل + +557 +00:37:24,790 --> 00:37:29,110 +في الحضانة نتحصله كل ست ساعات arterial blood gas + +558 +00:37:29,110 --> 00:37:31,510 +إيش يعني arterial blood gas؟ يعني ناخدله arterial + +559 +00:37:31,510 --> 00:37:35,070 +blood عشان ناخدله arterial blood بنحط إيدينا على + +560 +00:37:35,070 --> 00:37:40,090 +مكان ونعمل palpitation نحس وننبض لمام تبع الفمورة + +561 +00:37:40,090 --> 00:37:45,390 +الارترية و بعدين نمسح كهول و بعدين ندخل نيدل طبعا + +562 +00:37:45,390 --> 00:37:49,050 +لو مرض بالي بحط إصبعهتمام؟ و بمسحها فى كهول بنسى و + +563 +00:37:49,050 --> 00:37:52,690 +انا بقعد برجع كمان مرة أو بحطه القبرة كل ما اتعرض + +564 +00:37:52,690 --> 00:37:55,990 +الطفل مرارا و تكرارا لها ده العملية كل ما زاد فرصة + +565 +00:37:55,990 --> 00:37:59,710 +انه ايه؟ انه يصاب زى ما احنا كل ما روحنا على .. + +566 +00:37:59,710 --> 00:38:03,830 +على الجامعة بالمواصلة فرصة انه يصاب بحادث سيارة + +567 +00:38:03,830 --> 00:38:06,550 +بتبتعه لأ ليه بروح مرة ده؟ ليه بروح مرة تانية؟ + +568 +00:38:06,550 --> 00:38:11,500 +مظبط ولا لأ؟ فنفس الجثة كل واحد اتعرض لأعجبتكم + +569 +00:38:11,500 --> 00:38:16,440 +الفكرة؟ كل ما كبر واحد في السن فرصة ان ينصف بأمراض + +570 +00:38:16,440 --> 00:38:22,820 +معينة او بحوادث سيارات برضه بتكون اكبر طيب dry + +571 +00:38:22,820 --> 00:38:27,080 +surfaces زي human skin rare for a gram negative بس + +572 +00:38:27,080 --> 00:38:30,000 +الله يعني هذه الحادثة غيرها غالبا بنلاقيش على + +573 +00:38:30,000 --> 00:38:33,240 +الجلد تبقى على الجهة gram negative بس الله لكن + +574 +00:38:33,240 --> 00:38:36,350 +الأسنان دوبة أكتر بتكون موجودةoccasionally ممكن + +575 +00:38:36,350 --> 00:38:39,430 +تكون normal flora in the oropharynx يعني ممكن تكون + +576 +00:38:39,430 --> 00:38:42,990 +مولودة في الفموي الاند أكتر treatment prevention + +577 +00:38:42,990 --> 00:38:45,670 +and control antibiotic bacteria resistant is + +578 +00:38:45,670 --> 00:38:48,150 +common empirical treatment كلمة empirical + +579 +00:38:48,150 --> 00:38:52,230 +treatment يعني العلاج النظري وهذا النقطة اللي لازم + +580 +00:38:52,230 --> 00:38:56,570 +نفهمها ايش يعني علاج نظري؟ لو انا عرفت ان هذا + +581 +00:38:56,570 --> 00:39:03,080 +المريض عنده infection بقى سنة و بقتمعلوماتي ان + +582 +00:39:03,080 --> 00:39:06,060 +الاسنين التباكتر تستجيب ل antibiotic x و y و z + +583 +00:39:06,060 --> 00:39:12,480 +فبوصف لوحد من ال antibiotics دولة تمام بما يتلقى + +584 +00:39:12,480 --> 00:39:16,560 +مع المريض إذا كان أنثى حامل مش حامل طفل كبير مظبوط + +585 +00:39:16,560 --> 00:39:19,930 +فيه اعتبارات معينة صار في ال antibioticهذا بيسموه + +586 +00:39:19,930 --> 00:39:23,370 +empirical لكن لما أنا بعمل sensitivity testing + +587 +00:39:23,370 --> 00:39:26,210 +للأسنين تبقى أكتر و بقول له هذه sensitive لكذا و + +588 +00:39:26,210 --> 00:39:29,910 +resistant لكذا، بيبطل empirical، بيصير مبني على + +589 +00:39:29,910 --> 00:39:33,890 +اللي هو sensitivity testing، إذن ال empirical مبني + +590 +00:39:33,890 --> 00:39:36,890 +على معلومات نظرية، طب هذه المعلومات النظرية مش هي + +591 +00:39:36,890 --> 00:39:42,880 +متغيرة؟ بمعنى إنه في فلسطين غير عن أمريكافي العراق + +592 +00:39:42,880 --> 00:39:48,480 +غير عن سوريا لإن كل واحد له أسلوب في صرف الأدوية + +593 +00:39:48,480 --> 00:39:51,840 +ويله قوانينه تمام؟ ويله أصناع Antibiotics ممكن + +594 +00:39:51,840 --> 00:39:56,540 +تختلف عن الأخرى في البلدان التانية فواضح من ال .. + +595 +00:39:56,540 --> 00:40:00,220 +ال .. ال .. الكلام إن ال empirical therapy يجب أن + +596 +00:40:00,220 --> 00:40:07,000 +يكون مبني على بيانات .. بيانات محليةlocal جات عشان + +597 +00:40:07,000 --> 00:40:12,140 +انا بقول الدفاقة لكوارثات الصحة اللي فيها نتائج ال + +598 +00:40:12,140 --> 00:40:17,740 +sensitivity لو اتجمعت و اتصنفت و اتحللت و انبعتت + +599 +00:40:17,740 --> 00:40:22,660 +لطباء كل ست شهور بصير الدكتورة عارف جاتيش احتمالية + +600 +00:40:22,660 --> 00:40:26,240 +ان لو اعطى amoxicillin ان المرض يشفع من أسلوبه + +601 +00:40:26,240 --> 00:40:30,000 +بكتر، مظبوط ولا لأ؟ بس لما يكون مبني بياناته على + +602 +00:40:30,000 --> 00:40:34,890 +أوروبا أو على أمريكا او على textbook له عشرين سنةو + +603 +00:40:34,890 --> 00:40:40,470 +للأسف .. للأسف هذا ما يحدث في معظم الأحيان ليست + +604 +00:40:40,470 --> 00:40:44,830 +مبنية كمان مرة عالمين على local لأ و هذه نقطة + +605 +00:40:44,830 --> 00:40:49,190 +خطيرة جدا أنا بحد أنه أروح إليها باستمرار specific + +606 +00:40:49,190 --> 00:40:52,930 +therapy according to antibiotic susceptibility is + +607 +00:40:52,930 --> 00:40:56,790 +required أخر جملة في المحاضرة و أعتقد أن اليوم + +608 +00:40:56,790 --> 00:41:00,170 +بكفينا ان شاء الله نكمل المرة الجاية بالمراجز اللي + +609 +00:41:00,170 --> 00:41:00,950 +كتيرانس + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ.srt new file mode 100644 index 0000000000000000000000000000000000000000..3c649ef7d2683afbb4159da2a2622ee743d66810 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ.srt @@ -0,0 +1,2460 @@ +1 +00:00:21,890 --> 00:00:24,950 +بسم الله الرحمن الرحيم احنا وصفنا المحاضرة + +2 +00:00:24,950 --> 00:00:28,090 +الماضية عن تركيب من تركيب الخلية الداخلية اللي هو + +3 +00:00:28,090 --> 00:00:34,690 +الـ mesosome وبدنا نتعرف على مهيط الـ mesosome هو + +4 +00:00:34,690 --> 00:00:40,070 +عبارة عن invaginations نقول عنها هي بغى من مين؟ من + +5 +00:00:40,070 --> 00:00:44,210 +الـ cell، من الـ membrane، و الـ cell و الـ membrane فيه + +6 +00:00:44,210 --> 00:00:48,530 +وظائف عديدة، ما عرفتش أتركها لسه، لكن هذه + +7 +00:00:48,530 --> 00:00:56,630 +الوظائف إن هو عبارة عن إنتاج الطاقة وإن هو المقابل + +8 +00:00:56,630 --> 00:01:00,330 +لـ Mitochondria في الـ Eucaryotes فلو هناك نظرية + +9 +00:01:00,330 --> 00:01:05,970 +غريبة في التطور بتقول إن الـ Eucaryotic cell ما + +10 +00:01:05,970 --> 00:01:10,050 +كانت قادرة على إدارة الطاقة دخلت لها بكتيريا جوا + +11 +00:01:10,050 --> 00:01:16,690 +فالـ Eucaryote فرحت فيها وحولتها من بكتيريا إلى + +12 +00:01:16,690 --> 00:01:22,060 +Mitochondria هيك الأسطورة تتبع التطور عموما + +13 +00:01:22,060 --> 00:01:26,600 +الـ mesosome بيزيد مساحة السطح لأنه extension + +14 +00:01:26,600 --> 00:01:31,560 +للـ cell membrane وبالتالي أي وظيفة للـ cell membrane + +15 +00:01:31,560 --> 00:01:35,660 +هو بيزيد هذه الوظيفة والصورة اللي بتانكو هي عبارة + +16 +00:01:35,660 --> 00:01:39,640 +عن تشبيه واضح ما بين الـ mitochondria اللي هي الـ cristae + +17 +00:01:39,640 --> 00:01:41,700 +إذا فاكرين الـ cristae في تركيب اللي هي الأعراف + +18 +00:01:41,700 --> 00:01:46,000 +بالمقابل مع الـ invagination اللي احنا رسمناها هنا + +19 +00:01:46,000 --> 00:01:50,770 +أو هي ظاهرة هنابتزيد مساحة السطح وبتكون مكان مناسب + +20 +00:01:50,770 --> 00:01:54,710 +جدا لعملية اللي هو الـ Oxidative Phosphorylation و + +21 +00:01:54,710 --> 00:02:00,470 +Other Energy Producing Pathways خلصنا + +22 +00:02:00,470 --> 00:02:04,690 +الجدار الخلوي خلصنا الـ cell membrane ومتعلق بيه + +23 +00:02:04,690 --> 00:02:08,590 +كان الـ mesosome لأن دخلنا إلى داخل الخلية لا يدد + +24 +00:02:08,590 --> 00:02:12,250 +بيها إلا بحر من الـ cytoplasm و الـ cytoplasm مكون + +25 +00:02:12,250 --> 00:02:19,240 +mainly من water ماء مذاق في بعض الأملاح والسكريات + +26 +00:02:19,240 --> 00:02:23,220 +بالإضافة إلى أنه موجود داخله نوكليك أسيت بروتينات + +27 +00:02:23,220 --> 00:02:28,080 +and other compounds الآن من المراكبات أو من + +28 +00:02:28,080 --> 00:02:30,860 +التراكيب المهم جدا في الـ cytoplasm المتشهرة بشكل + +29 +00:02:30,860 --> 00:02:35,720 +كبير ليها الـ ribosomes و ribosomes أذكركم بأنها من نوع + +30 +00:02:35,720 --> 00:02:42,640 +70S الـ 70S اللي قصرناها الـ vaccine أو 50S 30S + +31 +00:02:42,640 --> 00:02:47,200 +وبنذكركم دائما بهذا المقولة لأنه معتمدين وهي أمكن + +32 +00:02:47,200 --> 00:02:54,070 +حتى يعني مذكورة هنا إنه هي المكان المستهدف لكثير من + +33 +00:02:54,070 --> 00:02:58,610 +الـ antibiotics الـ Macrolides، الـ Tetracyclines، + +34 +00:02:58,610 --> 00:03:02,130 +الـ gentamicin وعائلة كبيرة اسمها الـ Aminoglycosides + +35 +00:03:02,130 --> 00:03:07,310 +كلها بتستهدف لبروتين سينتيز إما على الـ 50S أو على + +36 +00:03:07,310 --> 00:03:11,410 +الـ 30S لذلك من المهم على الطلبة إنهم يكونوا عارفين + +37 +00:03:11,410 --> 00:03:16,030 +لبروتين سينتيز أساسا كيف بيتم عشان لما نيجي نشرح + +38 +00:03:16,030 --> 00:03:19,650 +كيف بنوقف لبروتين سينتيز نكون عارفين كيف أصلا + +39 +00:03:19,650 --> 00:03:24,320 +لبروتين سينتيز أساسا بيتم إن الـ ribosomes موزع على + +40 +00:03:24,320 --> 00:03:28,240 +كل الخلية تعتبر المصانع الرئيسية تصنيع الـ protein + +41 +00:03:28,240 --> 00:03:32,300 +في الخلية الـ plasmids هي تراكيب إضافية مهولة في + +42 +00:03:32,300 --> 00:03:35,440 +بعض البكتيريا مش في كل البكتيريا بينما الـ ribosome + +43 +00:03:35,440 --> 00:03:39,660 +لازم يكون في كل البكتيريا هنا قد تحتوي البكتيريا + +44 +00:03:39,660 --> 00:03:43,440 +على plasmid أو قد لا تحتوي على plasmid لكن هي + +45 +00:03:43,440 --> 00:03:48,960 +عبارة عن extra chromosomal DNA material يعني مادة + +46 +00:03:48,960 --> 00:03:54,260 +وراثية إضافية خارج عن طاقمين الكروموزوم هتكلم معاكم + +47 +00:03:54,260 --> 00:03:59,780 +شوية بتفاصيل أكثر و نعرف كيف تشتغل الـ plasmid الـ + +48 +00:03:59,780 --> 00:04:07,320 +inclusions الحبيبات نميل نحن إلى التخزين أشياء + +49 +00:04:07,320 --> 00:04:11,710 +ضرورية لنا خاصة لما تكون موجودة in abundance، يعني + +50 +00:04:11,710 --> 00:04:15,090 +بزيادة، تمام؟ في البيئة المحيطة فينا، بنحاول أنه + +51 +00:04:15,090 --> 00:04:18,790 +نخزنها، معانا عشرين دفتر، بناخد دفتر و بنخبه تسعة + +52 +00:04:18,790 --> 00:04:22,810 +عشر، عندنا أرضية معينة، بناخد جزء منها و بنخزنه + +53 +00:04:22,810 --> 00:04:27,370 +بالتلاجة أو بنخزنه كما يليق بنوع الطعام، إذا غالبا + +54 +00:04:27,370 --> 00:04:31,430 +الـ inclusion هي for storage، what type of storage؟ + +55 +00:04:31,430 --> 00:04:35,870 +غالبا، غالبا energy storage، والبكتيريا اللي ياخده + +56 +00:04:35,870 --> 00:04:43,690 +بالكم is a very wise when it is related to energy + +57 +00:04:43,690 --> 00:04:48,490 +يعني بمعنى لو البكتيريا دخلت وهي ميتة من الجوا + +58 +00:04:48,490 --> 00:04:53,230 +starving بمعنى إنها قعدت فترة طويلة بدون غذاء و + +59 +00:04:53,230 --> 00:04:57,190 +فجأة حاطناها في nutrient rich ما بتبدأ تاكل على + +60 +00:04:57,190 --> 00:05:01,270 +طول تبتطل على البسط، هي طبعا ماتشوفش زي احنا موز و + +61 +00:05:01,270 --> 00:05:06,710 +تفاح، هي ماتشوف glucose و lactose ودهون وبروتين + +62 +00:05:06,710 --> 00:05:10,310 +و carbohydrates فلو وردت على سبيل المثال جلوكوز + +63 +00:05:10,310 --> 00:05:15,510 +ولاكتوز ابتبدأ بالجلوكوز ليش؟ لأنه عندها انزيمات + +64 +00:05:15,510 --> 00:05:20,410 +هيك هيك منتجة للجلوكوز لو بدأت باللاكتوز مضطرة + +65 +00:05:20,410 --> 00:05:25,370 +تنتج انزيمات معينة كلمة تنتج يعني استهلاك الطاقة + +66 +00:05:25,370 --> 00:05:29,410 +فهي حكيمة جدا في استخدام الطاقة وإن كانت خلايا + +67 +00:05:29,410 --> 00:05:35,730 +صغيرة لكن هي very efficient factors الـ ribosomes زي + +68 +00:05:35,730 --> 00:05:40,830 +ما ذكرت لكم الـ complete ribosome هو 70S و 70S أو الـ + +69 +00:05:40,830 --> 00:05:43,790 +S هي sedimentation units اللي منها Svedberg unit + +70 +00:05:43,790 --> 00:05:48,570 +لما حصرناهم عن بعض وجدنا الـ large 50S و الـ small 30S و + +71 +00:05:48,570 --> 00:05:52,350 +الاثنين بيكونوا بالفعل مفصلين و بيصير لهم assembly + +72 +00:05:52,350 --> 00:05:55,670 +مع الـ mRNA و بتم عملية اللي هو الـ protein + +73 +00:05:55,670 --> 00:05:57,030 +synthesis + +74 +00:05:59,500 --> 00:06:03,420 +البروتين الـ synthesis بتتم في الخلايا بواسطة اللي + +75 +00:06:03,420 --> 00:06:06,500 +هو مين الـ ribosomes they translate the + +76 +00:06:06,500 --> 00:06:09,360 +information يعني بجانب الكلام أنا هذا information + +77 +00:06:09,360 --> 00:06:12,980 +مخزنة في المادة اللي ورا فيها أنا عندي information + +78 +00:06:12,980 --> 00:06:16,340 +مخزنة في الدماغ بنقلها لكم عبر اللسان وعبر + +79 +00:06:16,340 --> 00:06:20,580 +الحركات وعبر الصور بعض الأحيان عبر الفيديو لكن + +80 +00:06:20,580 --> 00:06:22,980 +البكتيريا ما بتتكلم لغتنا احنا اللغة اللي احنا + +81 +00:06:22,980 --> 00:06:28,980 +بنتكلم فيها بتتكلمها لغتها أبسط من ذلك لكن مطورة + +82 +00:06:28,980 --> 00:06:31,540 +احنا اليوم مبسوطين على حالنا معانا flash memory + +83 +00:06:31,540 --> 00:06:35,940 +مصبوح واحد صديق بيعطينا الـ flash بيقولنا خد هذا + +84 +00:06:35,940 --> 00:06:39,840 +بصلاحك الـ computer أو بنزلك ويندوز أنت مش عارف كيف + +85 +00:06:39,840 --> 00:06:42,520 +بنزل ويندوز أو كيف بيشيل الفيروس من الـ .. لكن + +86 +00:06:42,520 --> 00:06:47,630 +بيشيله بقطعة اللي هو الـ flash memory اللي عليها + +87 +00:06:47,630 --> 00:06:52,470 +information software تمام؟ نفس الشيء لما نشوف كمان + +88 +00:06:52,470 --> 00:06:55,430 +شوية الـ plasmids هنعرف كيف البكتيريا بتنقل + +89 +00:06:55,430 --> 00:06:58,490 +المعلومات من بكتيريا إلى أخرى عبر الـ information + +90 +00:06:58,490 --> 00:07:01,930 +تمام؟ يعني ما فيش داعي تعمل محاضرات للآخرين و + +91 +00:07:01,930 --> 00:07:06,530 +تحملهم جميلة لا بتعطيهم قطعة من المادة الوراثية و + +92 +00:07:06,530 --> 00:07:12,070 +انتهى الموضوع إذن they translate mRNA and produces + +93 +00:07:12,070 --> 00:07:16,590 +the information على شكل البروتين البروتين هو الـ + +94 +00:07:16,590 --> 00:07:20,390 +expression للمعلومات الموجودة في الـ RNA لأصل + +95 +00:07:20,390 --> 00:07:26,270 +المعلومة المأخوذة أساسا من الـ DNA للبلازميت أنا + +96 +00:07:26,270 --> 00:07:32,050 +حاولت التعريف أقطع قطعة صغيرة عشان أنزل المعلومات + +97 +00:07:32,050 --> 00:07:35,330 +هو البلازميت عبارة عن small circular extra + +98 +00:07:35,330 --> 00:07:41,750 +chromosomal خارج نطاق الكروموزوم double-stranded + +99 +00:07:41,750 --> 00:07:45,970 +DNA molecule في حد عنده شك إن الـ DNA أصلا double + +100 +00:07:45,970 --> 00:07:51,270 +-stranded ما هو double-stranded و لا حسيتوا شك مين + +101 +00:07:51,270 --> 00:07:55,490 +درس فيروسات ولا لسه محدش درس فيروسات في الفيروسات + +102 +00:07:55,490 --> 00:07:59,030 +هناك بعض الفيروسات المادة الوراثية بتكون single + +103 +00:07:59,030 --> 00:08:03,110 +-stranded DNA وبالتالي عملنا stressing يعني تأكيد + +104 +00:08:03,110 --> 00:08:06,910 +على كلمة double-stranded DNA molecule they are + +105 +00:08:06,910 --> 00:08:11,650 +capable of self-replicate تنقسم الـ .. الـ .. الـ + +106 +00:08:11,650 --> 00:08:15,550 +الـ plasmid independently of the chromosome يعني الـ + +107 +00:08:15,550 --> 00:08:19,450 +chromosome بنقسم لحال وهي تنقسم لحال ما فيش ارتباط + +108 +00:08:19,450 --> 00:08:24,110 +بينهم ما فيش ارتباط بينهم هل من المعقول إنه أنا + +109 +00:08:24,110 --> 00:08:27,070 +المعلومات اللي عندي في الدماغ إذا بده ينقلها لكم + +110 +00:08:27,070 --> 00:08:31,360 +إياها يعطيكم إياها وأبقى من غير المعلومات هذه ولا + +111 +00:08:31,360 --> 00:08:35,240 +لازم أعمل نسخ عنها بعمل نسخ وبعطيكم بحجم النسخ + +112 +00:08:35,240 --> 00:08:37,420 +اللي .. عشان أنا كنت بقول إنه الـ .. الـ .. الـ + +113 +00:08:37,420 --> 00:08:41,080 +plasmid هذه مثلا الـ plasmid عاملة replication في + +114 +00:08:41,080 --> 00:08:45,020 +أكثر من نسخة في الخلية هي واحدة اثنين ثلاثة أربعة + +115 +00:08:45,020 --> 00:08:50,720 +وهذا تمثيل قسمي لمين؟ للكروموزوم يعني إن التوضيح + +116 +00:08:50,720 --> 00:08:54,960 +للكروموزوم أكبر بكثير من مين من الـ plasmid كمان برضه + +117 +00:08:54,960 --> 00:08:59,540 +small circular extra chromosomal double stranded + +118 +00:08:59,950 --> 00:09:07,250 +DNA capable of self-replication and contains genes + +119 +00:09:07,250 --> 00:09:14,310 +تحتوي على جينات that confer تمنح تعطي some + +120 +00:09:14,310 --> 00:09:18,890 +properties such as antibiotic resistance تخيلوا أن + +121 +00:09:18,890 --> 00:09:23,190 +البكتيريا تتبادل المعرفة في كيفية حماية نفسها من + +122 +00:09:23,190 --> 00:09:28,850 +الـ antibiotic بكتيريا بطريقة ما صارت عندها المعرفة + +123 +00:09:30,060 --> 00:09:33,080 +بمقاومة الـ .. الـ .. الـ antibiotic اسمه Benzalic + +124 +00:09:33,080 --> 00:09:37,840 +كيف؟ عن طريق إنتاج أنزيم اسمه Penicillinase الـ + +125 +00:09:37,840 --> 00:09:41,600 +Penicillinase بيكسر الـ Penicillin فبالتالي بيصير + + +126 +00:09:41,600 --> 00:09:44,100 +الـ Benzaline غير فعال ضد البكتيريا وبالتالي + +127 +00:09:44,100 --> 00:09:47,260 +البكتيريا الآن protected من الـ Penicillin صح ولا + +128 +00:09:47,260 --> 00:09:51,200 +مش صح؟ طيب هذه المعلومات وين مخزنة؟ إنتاج الـ + +129 +00:09:51,200 --> 00:09:54,980 +Penicillinase على الـ plasma فلو نقلت البكتيريا + +130 +00:09:54,980 --> 00:09:59,870 +المقاومة للبلاسمة تبقى حال البكتيريا غير مقاومة + +131 +00:09:59,870 --> 00:10:04,990 +تصبح البكتيريا الأخرى مقاومة زي ما قلت لكوا الـ + +132 +00:10:04,990 --> 00:10:10,190 +flash حطيناها في جهاز تمام، أخذ من الـ flash معلومة + +133 +00:10:10,190 --> 00:10:13,690 +أخذ من الجهاز معلومة كيفية مكافحة فيروس معين، فيروس + +134 +00:10:13,690 --> 00:10:19,770 +حسوبي أخذناه وحطيناه في الجهاز الثاني، الجهاز ينسخ + +135 +00:10:19,770 --> 00:10:24,010 +إخلاء صفة أنه مقاوم لهذا الفيروس، طبعا مش بس + +136 +00:10:24,010 --> 00:10:28,550 +antibiotic resistance كمان ممكن تنقل جينات ذات + +137 +00:10:28,550 --> 00:10:32,190 +معلومات خاصة بإنتاج سموم على سبيل المثال، يعني + +138 +00:10:32,190 --> 00:10:37,490 +بكتيريا بريقة مايلها المقدرة على إنتاج سم لما + +139 +00:10:37,490 --> 00:10:42,150 +نقلناها لبلازميل صلاة تنتج هذا السم، أي مادة من + +140 +00:10:42,150 --> 00:10:48,130 +شأنها أن تجعل البكتيريا أكثر شراسة، أكثر إمراضة + +141 +00:10:48,130 --> 00:10:54,210 +بيسميها virulence factor أي عامل يجعل من البكتيريا + +142 +00:10:54,210 --> 00:10:59,130 +قادرة على إحداث المرض، بنسميه Virulence Factor مهم + +143 +00:10:59,130 --> 00:11:03,130 +كتير المعلومة هذه لأنها حد رح نكررها مرارا وتكرارا + +144 +00:11:03,130 --> 00:11:07,950 +Plasmids are not essential for the survival of the + +145 +00:11:07,950 --> 00:11:12,990 +cell بمعنى أنه مش ليس الـ wall ولا الـ cell membrane + +146 +00:11:12,990 --> 00:11:16,330 +ولا الـ ribosomes لو مافيش Plasmids ممكن تعيش + +147 +00:11:16,330 --> 00:11:20,150 +البكتيريا بشرط أنه تعيش في ظروف ملائمة + +148 +00:11:23,860 --> 00:11:27,500 +طب هتنتقل البلازميت من خلية لخلية؟ نعم هتنتقل، + +149 +00:11:27,500 --> 00:11:31,100 +هتتكلم عن حسمة conjugation اللي هي الاقتران + +150 +00:11:31,100 --> 00:11:36,640 +الجنسي، خلية مع خلية بتبادلوا المعلومات، أنا تحدثت + +151 +00:11:36,640 --> 00:11:39,920 +إن في الـ سترات البلازم، فينا inclusion bodies، وكنا + +152 +00:11:39,920 --> 00:11:44,240 +الـ inclusion bodies هي عبارة عن storage granules، + +153 +00:11:44,240 --> 00:11:48,000 +والغرض من الـ storage أو الداعي للـ storage هو الـ + +154 +00:11:48,000 --> 00:11:52,300 +abundance، اللي هو فيه كمية كبيرة موجودة من الغذاء + +155 +00:11:52,300 --> 00:11:58,870 +في المحيط، طبعا تخزن هذا الغذاء الزائد لحين الحاجة + +156 +00:11:58,870 --> 00:12:01,930 +تمام؟ فيه granules بيسموها storage granules أو بعض + +157 +00:12:01,930 --> 00:12:05,970 +الأحيان inclusions، طبعا الحديث عن هذا الموضوع + +158 +00:12:05,970 --> 00:12:08,670 +بيختلف لما أتكلم في الـ medical microbial بيختلف عن + +159 +00:12:08,670 --> 00:12:10,990 +... لما أتكلم عن الـ ... الـ ... الـ environmental في + +160 +00:12:10,990 --> 00:12:15,730 +بكتيريا عصيمتال بتخزن المغناطيسات، بيسموها + +161 +00:12:15,730 --> 00:12:20,390 +magnetosomes بتخزن في حالها، فبصير بكتيريا مغناطيسية + +162 +00:12:20,390 --> 00:12:24,430 +بيسموها magnetotactic bacteria المجنيدوتاكتك + +163 +00:12:24,430 --> 00:12:28,850 +بكتيريا تستخدم الميغناطيسات هذه في الحفاظ على + +164 +00:12:28,850 --> 00:12:33,210 +نفسها في مستوى معين في المياه مثلا في مياه البراكا + +165 +00:12:33,210 --> 00:12:36,970 +أو المياه العادمة، تمام؟ بدل ما تطفو على السطح أو + +166 +00:12:36,970 --> 00:12:40,730 +تستقر في القاع، بترتب الميغناطيسات بحاجة إنها تكون + +167 +00:12:40,730 --> 00:12:44,130 +في مستوى معين، هذا المستوى المعين ملائم إلا من + +168 +00:12:44,130 --> 00:12:50,310 +ناحية الأكسجين يعني وكأنه وكأنه وسيلة anchoring + +169 +00:12:50,310 --> 00:12:55,620 +مرسى، ترسي نفسها في مكان معين مفضل لها الظروف، فهناك + +170 +00:12:55,620 --> 00:13:00,600 +الكثير من الـ inclusion granules المتنوعة لكن + +171 +00:13:00,600 --> 00:13:04,780 +معظمها الغرض الأساسي منها إيه يا بنات؟ الـ storage + +172 +00:13:04,780 --> 00:13:10,900 +والـ storage تبقى إيه؟ تبقى energy molecules هذه + +173 +00:13:10,900 --> 00:13:13,920 +بعض الصور الـ electron microscope بتوضح لنا ما فينا + +174 +00:13:13,920 --> 00:13:17,640 +اللي هو inclusion bodies في كثير من أنواع من + +175 +00:13:17,640 --> 00:13:22,260 +البكتيريا أو granules، أحنا اتفقنا إن أصلا + +176 +00:13:22,260 --> 00:13:25,380 +البروكاريوتس لا تحتوي على نوكلياس، فاحنا حاطينا + +177 +00:13:25,380 --> 00:13:29,000 +عنوان نوكلياس عشان ننفيه فقط، لغة نقولكوا إنه + +178 +00:13:29,000 --> 00:13:33,640 +lacking nuclear membrane، absence of nuclei اللي هي + +179 +00:13:33,640 --> 00:13:38,320 +باقي نسميها نواة ونوية، هه، hence known as ... لذلك + +180 +00:13:38,320 --> 00:13:43,020 +النواة في البروكاريوتس تسمى nucleic material أو + +181 +00:13:43,020 --> 00:13:47,240 +نوكليايد، انسوا الدم الأخير هذا هو كان مش موجود + +182 +00:13:50,230 --> 00:13:53,990 +الآن الصورة اللي أمامكوا هذه، طلعولي عليها، حد + +183 +00:13:53,990 --> 00:14:01,050 +عنده تخيل إيش هذه الصورة؟ شايفينها أو مش شايفينها؟ + +184 +00:14:01,050 --> 00:14:04,650 +شجرة؟ + +185 +00:14:04,650 --> 00:14:13,770 +عفا مثلا خالد، تفضلي، هذه الـ chromosome تعلق شريشة + +186 +00:14:13,770 --> 00:14:20,690 +أكولية، لو فرضناه بيمشي + +187 +00:14:20,690 --> 00:14:24,530 +معانا من أول الجامعة لآخر الجامعة، والبكتيريا ليست + +188 +00:14:24,530 --> 00:14:30,350 +طولها واحد مايكروم، هذا الحجم وهذا التعقيد كله + +189 +00:14:30,350 --> 00:14:33,650 +موجود في واحد مايكروم + +190 +00:14:37,250 --> 00:14:40,410 +اللي أنا قلت لكوا هو single و circular ولما + +191 +00:14:40,410 --> 00:14:44,390 +البكتيريا بده تنتج ... بده تنتج إنزيم معين، ابتعد + +192 +00:14:44,390 --> 00:14:47,590 +تأمر للجين الوحيد اللي موجود لدى الانزيم من كل + +193 +00:14:47,590 --> 00:14:52,490 +هالخربة طيارة إنه يشتغل، small but terrible small + +194 +00:14:52,490 --> 00:14:58,210 +but efficient يعني صغيرة لكن رهيبة، صغيرة لكن فعالة + +195 +00:14:58,210 --> 00:15:03,390 +إياكم أن تنظروا للبكتيريا على إنها يعني مخلوق يعني + +196 +00:15:03,390 --> 00:15:09,730 +في السلسلة وانخفضت، البكتيريا عندها ... قلنا قبل شوية + +197 +00:15:09,730 --> 00:15:12,970 +في الـ energy أشطر من إطلاع الصحراء وبتعرف تنجي + +198 +00:15:12,970 --> 00:15:16,830 +أكلها أكتر من ما نختاره، بتعرف كيف تهرب من المواد + +199 +00:15:16,830 --> 00:15:21,450 +السامة، بتعرف كيف تتكثر بشكل فعال، كيف تخزن، يعني + +200 +00:15:21,450 --> 00:15:25,230 +كل المهارات اللي بيمتلكها البشر، تمام؟ ومن ضمنها + +201 +00:15:25,230 --> 00:15:31,890 +التعليم، تتعلم البكتيريا بشكل جيد من بعضها البعض، + +202 +00:15:31,890 --> 00:15:36,830 +single الـ chromosome, circular and compacted عشان + +203 +00:15:36,830 --> 00:15:39,530 +هيكن هذا اللي طوله من أول جامعة لأخر جامعة + +204 +00:15:39,530 --> 00:15:46,510 +بينضبط كله في واحد مايكن، نيجي لتركيب مهم جدا اسمه + +205 +00:15:46,510 --> 00:15:49,330 +الـ capsule أو الإصلاح الغلاف، وأنا بتمنى عليكم + +206 +00:15:49,330 --> 00:15:53,450 +إنكوا يعني اللوحة شوية تبقى قدوة عنها و تنتبهوا + +207 +00:15:53,450 --> 00:15:59,990 +لكلام اللي بتحكي، الغلاف + +208 +00:15:59,990 --> 00:16:04,290 +الخارجي اللي أول غلاف بحيط بالـ cell wall لما طلعنا + +209 +00:16:04,290 --> 00:16:12,400 +برا هو الـ capsule هذه الـ bacteria هذا + +210 +00:16:12,400 --> 00:16:15,580 +الـ structure اللي ورا الـ cell wall بنسميها الـ + +211 +00:16:15,580 --> 00:16:20,180 +capsule، العلماء احترفوا تسميته الصحيح يعني لو + +212 +00:16:20,180 --> 00:16:22,980 +طلعتوا على الكتب بتلاقيه capsule، glycocalyx، + +213 +00:16:22,980 --> 00:16:26,660 +slime layer، S layer، micro capsule، طب الاختلافات + +214 +00:16:26,660 --> 00:16:30,080 +هذه إيش كانت سببها؟ الاختلافات هذه كانت سببها + +215 +00:16:30,080 --> 00:16:34,680 +شغلتين رئيسيتين، الـ thickness سماكة + +216 +00:16:36,130 --> 00:16:45,270 +الطبقة وانتظامها، السماكة ماسميها thickness و الـ + +217 +00:16:45,270 --> 00:16:49,170 +organization اللي + +218 +00:16:49,170 --> 00:16:55,150 +هو تنظيم الكتشوف لحظة هذا regular منتظم، لو تخيلنا + +219 +00:16:55,150 --> 00:17:01,850 +تركيب آخر عامل زي هى بنفس الـ thickness هذا + +220 +00:17:01,850 --> 00:17:09,210 +منتظم مش منتظم، فهل نسمي هذا نفس اسم الكابسول ولا ما + +221 +00:17:09,210 --> 00:17:13,130 +نسموهاش؟ فقال صار في عنا شبه اتفاق، يمكن مش مذكور + +222 +00:17:13,130 --> 00:17:17,750 +قدرنا هنا، حاجة بيسموها glycocalyx أي تركيب، أي + +223 +00:17:17,750 --> 00:17:22,910 +تركيب بينطبق على صفات الكابسول هذه اللي هنا بيسموه + +224 +00:17:22,910 --> 00:17:25,530 +glycocalyx، لكن بيسموه glycocalyx إلى أربعة أصلا، + +225 +00:17:25,530 --> 00:17:29,890 +خلينا نكتبهم على الصرف، glyco + +226 +00:17:35,680 --> 00:17:40,820 +الجليجوكاليكس قد يكون واحد + +227 +00:17:40,820 --> 00:17:45,620 +من أربعة، capsule، micro + +228 +00:17:45,620 --> 00:17:51,420 +capsule، slime + +229 +00:17:51,420 --> 00:17:55,460 +layer، و + +230 +00:17:55,460 --> 00:17:58,960 +S layer، على لزج يعني طبقة لزجة وهي كله أصلا لزجة + +231 +00:17:58,960 --> 00:18:03,000 +هنشوف كمان شوية الوضعات تبعتها، و S layer + +232 +00:18:06,040 --> 00:18:13,140 +الآن مال التشابه ومال الاختلاف بين الأربع هدول واضح + +233 +00:18:13,140 --> 00:18:16,240 +كابسول وكابسول في بينهم علاقة، و layer و layer برضه + +234 +00:18:16,240 --> 00:18:19,480 +فيهم علاقة، لعلاقة اللي بينهم هدول لإنه تنتين + +235 +00:18:19,480 --> 00:18:24,320 +organized، بينما + +236 +00:18:24,320 --> 00:18:26,680 +الـ slime layer لعلاقة اللي بينهم إنه none + +237 +00:18:26,680 --> 00:18:34,080 +organized، فبدا هذا الشيء الـ common إيش الاختلاف؟ + +238 +00:18:35,450 --> 00:18:44,370 +الـ Thickness يعني + +239 +00:18:44,370 --> 00:18:48,750 +الكابسول organized والمايكرو كابسول organized، واضح + +240 +00:18:48,750 --> 00:18:52,330 +أن كلمة كابسول هي الأكبر فالـ thickness يبقى عالي + +241 +00:18:52,330 --> 00:18:55,390 +المايكرو كابسول organized بس إيه الـ thickness صغير + +242 +00:18:55,390 --> 00:19:00,670 +الـ slime layer مش organized لكن thick، الـ S layer + +243 +00:19:00,670 --> 00:19:05,060 +مش organized لكن thin، وهي الفروقات بين الأربعة، + +244 +00:19:05,060 --> 00:19:10,440 +إذا لو سمعتوا في يوم من الأيام مصطلح glycocalyx، + +245 +00:19:10,440 --> 00:19:14,020 +capsule ولا micro capsule ولا S layer ولا slam + +246 +00:19:14,020 --> 00:19:18,360 +layer، كلهم في النهاية هم اللي أنتو بتعرفوهم باسم + +247 +00:19:18,360 --> 00:19:22,800 +مين؟ باسم capsule، باسم capsule، لكن معنين إنه + +248 +00:19:22,800 --> 00:19:26,700 +نقلناه، كتير من الكتب صارت تتكلم عنهم بضرر مختلفة ومتنوعة، + +249 +00:19:26,700 --> 00:19:29,920 +فكويس إن أنتو عارفين هذه المعلومة + +250 +00:19:32,340 --> 00:19:36,920 +ما هي ... آه مما تتكون الكابسول هذه نقطة كتير مهمة + +251 +00:19:36,920 --> 00:19:47,240 +ايش التركيب الكيميائي للكابسول مكتوب من اللوحة من + +252 +00:19:47,240 --> 00:19:52,220 +polysaccharide لكن هناك exception هناك exception + +253 +00:19:52,220 --> 00:19:58,600 +هي الـ polysaccharide رأيتها؟ when however certain + +254 +00:19:58,600 --> 00:20:03,080 +bacilli they are composed of polypeptide وأجيبلكم + +255 +00:20:03,080 --> 00:20:07,960 +مثال صارخ على هذه البكتيريا عشان تحفظوا اسمها، كم + +256 +00:20:07,960 --> 00:20:12,000 +اسم البكتيريا اللي تحفظينها اليوم؟ ولا واحدة؟ + +257 +00:20:22,290 --> 00:20:27,490 +Bacillus anthracis وحي المسبب لجامرة الخبيثة جامرة + +258 +00:20:27,490 --> 00:20:32,390 +الخبيثة مرض خطير جدا أمريكا أتهمت العراق في حرب + +259 +00:20:32,390 --> 00:20:36,930 +الخليج الأول والثانية على أنه تمتلك مئات الأطنان + +260 +00:20:36,930 --> 00:20:38,790 +من هذه البكتيريا وأنه هتستخدمها في السلاح + +261 +00:20:38,790 --> 00:20:44,450 +البيولوجي فبكتيريا خطيرة الـ capsule تبعتها تتكون من + +262 +00:20:44,450 --> 00:20:48,870 +polypeptide خلافا لمعظم أنواع البكتيريا المتكونة + +263 +00:20:48,870 --> 00:20:55,750 +من مين قلنا من polyهتذكركم الـ أربعة شريشيا كولاي، + +264 +00:20:55,750 --> 00:20:59,270 +Staphylococcus aureus مايكوبلازما اللي ملاقش cell + +265 +00:20:59,270 --> 00:21:03,010 +wall تمام من بارح وهي البكتيريا اللي احنا بنتكلم + +266 +00:21:03,010 --> 00:21:11,590 +عنها الـ Bacillus anthracis أنصحكم بالمتابعة أولا بأول + +267 +00:21:11,590 --> 00:21:17,490 +حتى لا تتركن المعلومات اليوم بعض + +268 +00:21:17,490 --> 00:21:22,950 +الصور للـ capsule تبوا معاياهذه صورة electron + +269 +00:21:22,950 --> 00:21:27,350 +microscope تظهر انها كابسول الـ capsule أنا أظهرت + +270 +00:21:27,350 --> 00:21:32,510 +انها irregular صح هذه irregular مرة هنا سميكة و + +271 +00:21:32,510 --> 00:21:38,190 +مرة هنا رقيقة فهذه نسميها إما slim layer أو s + +272 +00:21:38,190 --> 00:21:42,990 +layer حسب المقارنة في الـ thickness بينما هذه رحظة + +273 +00:21:42,990 --> 00:21:50,270 +regular لكن thin فبسموها micro caps الصورتين هدولة + +274 +00:21:50,560 --> 00:21:53,640 +هم عبارة عن صورتين هتشوفوهم في المعمل إن شاء الله + +275 +00:21:53,640 --> 00:21:56,760 +لما تشتغلوا الـ capsule stain يعني في احنا في + +276 +00:21:56,760 --> 00:22:02,100 +المعمل جزئية متعلقة بصبغة الـ capsule فهنشوف + +277 +00:22:02,100 --> 00:22:07,200 +البكتيريا عبارة عن حاجة hallow أو dark وحواليها + +278 +00:22:07,200 --> 00:22:11,540 +منطقة hallow يعني نفس البكتيريا هتكون dark لأنه + +279 +00:22:11,540 --> 00:22:14,820 +بنستخدم ما يسمى بالـ negative stain الـ negative + +280 +00:22:14,820 --> 00:22:20,140 +stain يعني بنصبغ الخلفية وبنصبغش الخلية نفسها + +281 +00:22:20,140 --> 00:22:23,320 +الخلفية تبعت البكتيريا بتصبغ بينما البكتيريا + +282 +00:22:23,320 --> 00:22:28,500 +لاتصبغ لأن حضرة الـ capsule على الرغم من أنها + +283 +00:22:28,500 --> 00:22:36,340 +virulence factor مهم جدا اللي انها delicate رقيقة، + +284 +00:22:36,340 --> 00:22:40,940 +هشة، سهل لو عملناها بالمعالجات فكل gram is 10؟ + +285 +00:22:40,940 --> 00:22:44,620 +crystal violet و iodine و Ethanol و بعدين سفرانين، + +286 +00:22:44,620 --> 00:22:48,840 +هذه معالجات صعبة على البكتيريا، لو الـ capsule موجودة + +287 +00:22:48,840 --> 00:22:52,880 +ستفقد الـ capsule، فنعالجها بطريقة معينة هنشوفها في + +288 +00:22:52,880 --> 00:22:58,880 +المعمل إن شاء الله الآن نيجي للـ functions of the + +289 +00:22:58,880 --> 00:23:02,920 +capsule، وركزوا كويس، لإن الـ functions هذه كتير + +290 +00:23:02,920 --> 00:23:09,730 +مهمة، رقم واحد، attachment هنشوف function عكسها + +291 +00:23:09,730 --> 00:23:14,190 +تماما للـ capsule عكس الـ attachment هروب من الـ + +292 +00:23:14,190 --> 00:23:19,170 +attachment مظبوط ان الـ attachment التصاق بنا + +293 +00:23:19,170 --> 00:23:23,490 +تخيلوا معي التخيل هذا الـ urinary tract بتبتدأ من + +294 +00:23:23,490 --> 00:23:27,550 +وين؟ من الـ kidneys مظبوط وبتروح الحالب والحالب + +295 +00:23:27,550 --> 00:23:30,150 +بيصف فيه اللي هو الـ .. الـ .. الـ .. الـ urinary + +296 +00:23:30,150 --> 00:23:33,410 +bladder وبعدين الـ .. الـ .. الـ .. الخارج لو + +297 +00:23:33,410 --> 00:23:38,080 +بكتيريا جاءت تتسلق في الـ ureter قطرة بول أعتقد + +298 +00:23:38,080 --> 00:23:41,860 +قطرة البول عارفين حاجة من قطرة طبعا قطرة البول + +299 +00:23:41,860 --> 00:23:45,740 +جداش حجب من المقارنة مع البكتيريا كبير القطرة اللي + +300 +00:23:45,740 --> 00:23:50,900 +ضخمة صح البكتيريا صغيرة خالص فلو نزلت عليها قطرة + +301 +00:23:50,900 --> 00:23:55,940 +تشبيه زي ما واحد جاعت تحت شلالة نياجرة طبعا واحد + +302 +00:23:55,940 --> 00:24:01,060 +واحد منها بيصير بترد الحشرة فبالتالي عملية سقوط + +303 +00:24:01,060 --> 00:24:06,850 +البول من أعلى بتطرد البكتيريا إلى أسفل إلا إذا كان + +304 +00:24:06,850 --> 00:24:12,290 +البكتيريا عندها المقض على إيش؟ على الـ ureter يبقى لن + +305 +00:24:12,290 --> 00:24:16,750 +يحدث urinary tract infection التهابات مسالك بولية + +306 +00:24:16,750 --> 00:24:21,490 +إلا إلا دي أنواع من البكتيريا تمتلك المقض على مين؟ + +307 +00:24:21,490 --> 00:24:26,170 +على الـ ureter يبقى الـ virulence factor essential + +308 +00:24:26,170 --> 00:24:30,950 +أساسي مهم جدا جدا ان نقول البكتيريا عندها المقض + +309 +00:24:30,950 --> 00:24:33,270 +على الالتصاق طيب + +310 +00:24:36,380 --> 00:24:39,380 +لما ندرس diagnostic microbiology أو حتى في الـ .. + +311 +00:24:39,380 --> 00:24:42,500 +في الـ medical microbiology إن شاء الله، هنتعرف على + +312 +00:24:42,500 --> 00:24:46,760 +بعض المصطلحات منها infection، مصطلح infection، + +313 +00:24:46,760 --> 00:24:49,600 +هتلاقوا ان الـ infection عبارة عن successful + +314 +00:24:49,600 --> 00:24:54,180 +colonization، طب إيش colonization؟ successful + +315 +00:24:54,180 --> 00:24:59,000 +existence في مكان لم تكن مولد فيه بكتير سابقا الـ + +316 +00:24:59,000 --> 00:25:01,800 +Successful هذا مع الـ Successful هذا بيقدّوا إلى + +317 +00:25:01,800 --> 00:25:05,720 +أو قد يقدّوا إلى disease لكن لن يكون هناك success + +318 +00:25:05,720 --> 00:25:09,860 +إلا ده كان فيه إمكانية attachment البكتيريا عشان + +319 +00:25:09,860 --> 00:25:14,640 +تتغذى لازم تلتصق إلا إذا كان في بيئة سائل، بيئة + +320 +00:25:14,640 --> 00:25:19,640 +جسد بتلتصق على خلايا عشان تتغذى على هذه الخلايا + +321 +00:25:19,640 --> 00:25:25,110 +لأن التصاق عملية مهمة these structures are thought + +322 +00:25:25,110 --> 00:25:28,110 +to be to help cell attached to their target + +323 +00:25:28,110 --> 00:25:31,870 +environment target environment يعني و هذا و هذا + +324 +00:25:31,870 --> 00:25:34,210 +أخذوا بأكوا الـ attachment بيكون عبارة عن receptor + +325 +00:25:34,210 --> 00:25:38,430 +receptor يعني مش التصاق لإنه الـ .. لإنه الـ .. الـ + +326 +00:25:38,430 --> 00:25:43,490 +.. الـ capsule لذجة لأ الاتصاق بيكون عبر إيه؟ + +327 +00:25:43,490 --> 00:25:51,090 +receptor يعني فيها receptor للأنزل مثال عليها بصحى + +328 +00:25:51,090 --> 00:25:56,990 +اسم جديد mutants produces a slime layer in the + +329 +00:25:56,990 --> 00:26:02,510 +presence of sucrose اللي بتشوفوا أصغار أطفال ماراش + +330 +00:26:02,510 --> 00:26:06,410 +على المدرسة و أسنانه كلها مسوسة مظبوط تسأل أمه + +331 +00:26:06,410 --> 00:26:10,390 +بيقولك هذه ما هو بيأكلش غير حلو الحلو رايش أنه حلو + +332 +00:26:10,390 --> 00:26:16,890 +سكر و الـ سكر اللي بنعرفه سكروز فالسكروز اللي هو + +333 +00:26:16,890 --> 00:26:20,460 +الـ سكر لو البكتيريا اللي اسمها streptococcus mutants + +334 +00:26:20,460 --> 00:26:25,820 +وهي تعيش بالفن بالمناسبة حضرتها في وجود الـ .. في + +335 +00:26:25,820 --> 00:26:28,480 +وجود الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +336 +00:26:28,480 --> 00:26:28,500 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +337 +00:26:28,500 --> 00:26:28,900 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +338 +00:26:28,900 --> 00:26:29,320 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +339 +00:26:29,320 --> 00:26:29,860 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +340 +00:26:29,860 --> 00:26:30,000 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +341 +00:26:30,000 --> 00:26:30,400 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +342 +00:26:30,400 --> 00:26:30,620 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +343 +00:26:30,620 --> 00:26:31,020 +الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ + +344 +00:26:31,020 --> 00:26:32,880 +.. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. الـ .. + +345 +00:26:32,880 --> 00:26:38,720 +الـ .. الـ .. الـ .. الـ .. الـ .. + +346 +00:26:38,720 --> 00:26:39,720 +الـ .. + +347 +00:26:48,350 --> 00:26:52,010 +and many bacteria can stick to tooth surfaces and + +348 +00:26:52,010 --> 00:26:52,830 +cause decay + +349 +00:27:00,170 --> 00:27:03,990 +لما بتتكاثر البكتيريا وبتتغذى على الـ carbohydrate + +350 +00:27:03,990 --> 00:27:09,830 +بتنتج acid، الـ acid بيتفاعل مع الـ calcium اللي + +351 +00:27:09,830 --> 00:27:13,930 +موجود في الأسنان، فبياخد الـ calcium من الأسنان، + +352 +00:27:13,930 --> 00:27:16,110 +لما ياخد الـ calcium من الأسنان هو مين اللي بيخلي + +353 +00:27:16,110 --> 00:27:20,310 +الأسنان قوية و صلبة؟ الـ calcium، وبالتالي تبدأ هنا + +354 +00:27:20,310 --> 00:27:24,990 +التشوهات أو التسوس اللي احنا بنسميه تسوس الأسنان + +355 +00:27:24,990 --> 00:27:28,790 +أو ما يسمى بـ dental caries، dental caries، تسوس + +356 +00:27:28,790 --> 00:27:34,830 +الأسنان خدنا مثال على مين؟ على الـ streptococcus + +357 +00:27:34,830 --> 00:27:39,050 +mutants في مثال تاني على حسب الـ Vibrio cholera و + +358 +00:27:39,050 --> 00:27:42,070 +الـ Vibrio cholera من اسمها بتسبب مرض الكوليرا + +359 +00:27:42,070 --> 00:27:47,250 +إسهال شديد جدا يقتل الأطفال في كثير من البلدان في + +360 +00:27:47,250 --> 00:27:50,950 +العالم وعندنا في فلسطين حدثت مجموعة من الأوبئة + +361 +00:27:50,950 --> 00:27:54,890 +إلى الكوليرا لكن ربنا سلم يعني ما كان فيه نسبة + +362 +00:27:54,890 --> 00:28:00,870 +انتشار واسع also produces a glycocalyx which helps + +363 +00:28:00,870 --> 00:28:04,830 +to attach to the intestinal villi in the host يعني + +364 +00:28:04,830 --> 00:28:09,690 +بأهلها ترتبط في الـ intestinal villi داخل العقل + +365 +00:28:09,690 --> 00:28:15,310 +وإلا وإلا مع الإسهال كل الـ vibro هتطلع برا، صح + +366 +00:28:15,310 --> 00:28:19,590 +ولا؟ وبتلبط للـ disease، وخد بالك الإسهال أصلا هو + +367 +00:28:19,590 --> 00:28:23,970 +ميكانيزم من الجسم دفاعية للطرد، لكن في كثير من + +368 +00:28:23,970 --> 00:28:27,010 +الأحيان بتزيد الردد الفعل من عينا لدرجة أنها + +369 +00:28:27,010 --> 00:28:31,190 +تستهلك، تستهلك الـ .. الـ .. الـ electrolytes والماء + +370 +00:28:31,190 --> 00:28:35,630 +فبموت .. بموت الشخص مش من البكتيريا والصوم تبعها و + +371 +00:28:35,630 --> 00:28:38,590 +أنها مرض الفعل تبعاتنا الطبيعية ما نسميه الجفاف و + +372 +00:28:38,590 --> 00:28:41,110 +.. و .. و اختلال الـ electrolyte اللي بيسموه + +373 +00:28:41,110 --> 00:28:45,730 +electrolyte imbalance يبقى اتفاقنا على function + +374 +00:28:45,730 --> 00:28:49,770 +آخر واحد اسمها attachment نيجي بالتانية protection + +375 +00:28:49,770 --> 00:28:53,890 +from phagocytic engulfing كل كوب ماد غسلته بصابون + +376 +00:28:53,890 --> 00:28:58,630 +ايديك بصابون مش سائل صابون قطعوكلكوا أكيد في + +377 +00:28:58,630 --> 00:29:02,310 +حياتكوا مرة عليكم بتكونوا أول من يفتح الصابون + +378 +00:29:02,310 --> 00:29:05,330 +بينكوا بتشيل ورد عنها صح ولا لأ؟ إذا بتشيل ورد + +379 +00:29:05,330 --> 00:29:10,390 +بتمسكيها بتجع منك، لكن أولادكوا هتتبلل، وأول + +380 +00:29:10,390 --> 00:29:13,930 +حركتين اللي الصابون نقطة من هنا أو قفزت هنا، + +381 +00:29:13,930 --> 00:29:19,170 +مظبوط؟ ليش؟ لأنها أصبحت لزجة تخيلوا البكتيريا، هي + +382 +00:29:19,170 --> 00:29:22,830 +البكتيريا، وعليها الطبقة اللزجة، بتجي فاجوسايت ساعت؟ + +383 +00:29:23,010 --> 00:29:26,930 +بتلتقمها، ما بتلتقمها، بتضغط عليها اللي هي هاربة + +384 +00:29:26,930 --> 00:29:31,230 +منها، فأهم وضع .. عكس الـ attachment صح؟ الـ + +385 +00:29:31,230 --> 00:29:33,350 +attachment كانت عبارة عن يجب أن يكون receptor to + +386 +00:29:33,350 --> 00:29:37,690 +receptor، هنا لأنها لزجة هاربة من الفاجوسيتوس، و + +387 +00:29:37,690 --> 00:29:43,010 +أنا هنا مضطر أجفّ وأوضح نقطة أساسية في تعامل + +388 +00:29:43,010 --> 00:29:45,590 +الإنسان أو البكتيريا مع الإنسان أو الحيوان أو مع + +389 +00:29:45,590 --> 00:29:46,290 +الـ host يعني + +390 +00:29:50,840 --> 00:29:55,920 +أنتم الآن equal من ناحية الشراسة، تمام؟ لا تمتلكون + +391 +00:29:55,920 --> 00:29:58,920 +عوامل شراسة غير أسنانكم، وفي المدارس وأنتم أصحاب + +392 +00:29:58,920 --> 00:30:03,700 +الشعب، مظبوط؟ الآن عوامل الشراسة، نفترض أننا + +393 +00:30:03,700 --> 00:30:05,740 +أعطينا زميلتكم مسدس، لأن أنتم عارفين أننا نبيئة + +394 +00:30:05,740 --> 00:30:12,500 +نبيئة الـ مسدس هذا، الآن المسدس هذا أعطاها قدرة على + +395 +00:30:12,500 --> 00:30:15,960 +إحداث ضرر للآخرين، نسمي المسدس عامل شراسة، + +396 +00:30:15,960 --> 00:30:20,520 +أعطيناها سكين، برضه أعطناها عامل قوة، عامل شراسة، + +397 +00:30:20,520 --> 00:30:25,040 +سميناها violence factor، أعطيناها واقع لرصاص، يبقى + +398 +00:30:25,040 --> 00:30:29,400 +أعطيناها إيش الآن؟ عامل شراسة للحماية من المسدس، + +399 +00:30:29,400 --> 00:30:31,840 +وبالتالي برضه عامل شراسة، أعطتها قوة، أصلاً + +400 +00:30:31,840 --> 00:30:35,860 +اتّهاجموا يا مش خايفة، أمان؟ اللي أنا دقّقت .. اللي + +401 +00:30:35,860 --> 00:30:40,080 +أنا أوضحتُه إنه في أسلحة كانت هجومية، مسدس، سكين، + +402 +00:30:40,080 --> 00:30:44,150 +وكان فيه إيش الآن أنا؟ أسلحة دفاعية، الحرب بيننا + +403 +00:30:44,150 --> 00:30:49,930 +وبين البكتيريا بهذه البساطة هي تمتلك أسلحة هجومية + +404 +00:30:49,930 --> 00:30:54,410 +وأسلحة دفاعية ونحن كذلك نمتلك أسلحة هجومية والدليل + +405 +00:30:54,410 --> 00:30:58,430 +على ذلك أننا الفاجوسايتس والماكروفاج ابتلعت + +406 +00:30:58,430 --> 00:31:02,870 +وابتقتل صح؟ هي عندها الـ capsule اللي تهرب عندها + +407 +00:31:02,870 --> 00:31:06,410 +الـ capsule اللي تهرب عندها الـ toxin اللي تقتل عندها + +408 +00:31:06,410 --> 00:31:09,790 +أسلحة هجومية toxin وعندها أسلحة دفاعية مثلاً زي + +409 +00:31:09,790 --> 00:31:15,060 +اللي هو الـ capsule، وسنرى في الحرب فيه تمويه وخداع + +410 +00:31:15,060 --> 00:31:19,840 +البكتيريا هناك أنواع منها رائعة جداً في الخداع + +411 +00:31:19,840 --> 00:31:26,540 +disguise يعني ماكرة في الهروب من جهاز المناعة زي ما + +412 +00:31:26,540 --> 00:31:32,820 +كان في بعض الاجتياحات سير في دخول من بيت لبيت دون + +413 +00:31:32,820 --> 00:31:35,680 +الخروج للشارع كان يهدموا البيت ويخرجوا للبيت + +414 +00:31:35,680 --> 00:31:39,350 +الآخر لماذا؟ لأن في مقاومين في الشارع، فلو خرجنا من + +415 +00:31:39,350 --> 00:31:43,870 +الشارع في عرضة، هناك أنواع من البكتيريا تنتقل من + +416 +00:31:43,870 --> 00:31:48,790 +خلية إلى خلية دون الخروج منها تمام؟ بدون تدمير + +417 +00:31:48,790 --> 00:31:54,450 +الخلية الأولى كمان يعني اختراق جزء بسيط وتستطيع + +418 +00:31:54,450 --> 00:31:57,270 +.. هذا هروب من جهاز المناعة وليس هروب .. هروب من + +419 +00:31:57,270 --> 00:32:02,230 +جهاز المناعة، إذا بدنا نفهم أن عملية الـ pathogenesis + +420 +00:32:02,230 --> 00:32:06,990 +في البكتيريا هي عملية war مفتوحة حرب مفتوحة بين + +421 +00:32:06,990 --> 00:32:10,750 +الخلايا جهاز المناعة و .. و .. واخذوا بالكم، واحنا + +422 +00:32:10,750 --> 00:32:16,170 +دايماً غالبين، دايماً فايزين، متى بنهزم؟ لما نشعر أن + +423 +00:32:16,170 --> 00:32:21,570 +احنا مرضنا؟ بنقول أن هزمنا، أما الآن المعركة شغالة + +424 +00:32:21,570 --> 00:32:26,220 +في أفواهكم، في أمعائكم، في الجلد تبعكم، شغال المعركة + +425 +00:32:26,220 --> 00:32:30,160 +لأن أسلحة وعندهم أسلحة وطيبهم بيشتغلوا، إحنا ما + +426 +00:32:30,160 --> 00:32:33,560 +بيشتغلوش بيد المعركة إلا عندما نمرض ونكون في هذه + +427 +00:32:33,560 --> 00:32:36,900 +الحالة البكتيريا أو الفيروس قد فازت على جهاز + +428 +00:32:36,900 --> 00:32:41,200 +المناعة، إذا اتفقنا على أنه فإننا إيش لغاية؟ فإننا + +429 +00:32:41,200 --> 00:32:44,080 +viral infection اسمه capsule لأنه بيهرب من مين؟ من + +430 +00:32:44,080 --> 00:32:46,700 +الفاجوسايتس أو الـ viral infection هو باب الـ + +431 +00:32:46,700 --> 00:32:50,540 +attachment صح؟ بدون attachment صعبة البكتيريا تنجح + +432 +00:32:50,540 --> 00:32:56,050 +تظل في مكانها، للطبيعي، من أشهر الميكروبات اللي بتدل + +433 +00:32:56,050 --> 00:32:58,990 +عليها القضية الأمنية حاجة اسمها Streptococcus + +434 +00:32:58,990 --> 00:33:02,650 +pneumonia، ومن اسمها pneumonia تعمل التهاب نيومونيا + +435 +00:33:02,650 --> 00:33:06,330 +ركّزوا معايا في هذه الجرعة، في صنفين من هذه + +436 +00:33:06,330 --> 00:33:11,110 +البكتيريا صنف له capsule وصنف ما لهش capsule، لما + +437 +00:33:11,110 --> 00:33:17,150 +حقنهم في فارين، اللي إلها capsule قتلت الفار واللي + +438 +00:33:17,150 --> 00:33:21,290 +ما لهاش capsule هو كأنه ما حقناش الفار بحاجة، عاش + +439 +00:33:21,290 --> 00:33:25,620 +الفار، تمام؟ يبقى إيش كان الفرق بين الاتنين؟ وجود + +440 +00:33:25,620 --> 00:33:29,080 +الـ capsule، يبقى الـ capsule كانت مسئولة في هذه + +441 +00:33:29,080 --> 00:33:32,280 +البكتيريا عن إنها تكون pathogenic أو non + +442 +00:33:32,280 --> 00:33:38,820 +pathogenic، هذا مثال صارخ على أهمية الـ capsule في الـ + +443 +00:33:38,820 --> 00:33:43,200 +virus هذا + +444 +00:33:43,200 --> 00:33:46,140 +الجزء نفسه بيننا اللي فوق ما فيه أي إذن + +445 +00:33:46,140 --> 00:33:52,560 +function، ثلاثة resistance to dry، يعني فين أنت مقاوم + +446 +00:33:52,560 --> 00:33:55,100 +لمين؟ للجفاف، ركّزوا معايا في هذا الـ Disease إيه + +447 +00:33:55,100 --> 00:33:58,740 +بقى الآن إحنا لما Stripped Coccy pneumonia وأن + +448 +00:33:58,740 --> 00:34:03,180 +موجودة في الـ lungs بعتنا عامة إنها Disease، لما + +449 +00:34:03,180 --> 00:34:10,400 +بنكح مش بنطلع مع الكحة البكتيريا بنشوفها في البيئة + +450 +00:34:10,400 --> 00:34:15,080 +لو البكتيريا جفت تمام، الجفاف أحد عوامل القتل + +451 +00:34:15,080 --> 00:34:18,760 +للبكتيريا، بنجففها بنموتها، نشيل منها الميه بنموتها + +452 +00:34:20,540 --> 00:34:26,080 +فوجود capsule يجعل من تجفيف البكتيريا أمر ليس + +453 +00:34:26,080 --> 00:34:31,640 +بالسهل، وبالتالي يعني بتوفر برضه حماية للبكتيريا في + +454 +00:34:31,640 --> 00:34:36,920 +البيئة، كل ما طولت في البيئة كل ما زادت فرصتها + +455 +00:34:36,920 --> 00:34:42,140 +لانتقال لأشخاص آخرين صح ولا لأ؟ يعني نفترض أنه + +456 +00:34:42,140 --> 00:34:47,560 +البكتيريا قدرتها على البقاء تلات ساعات، أنا كحيت + +457 +00:34:47,560 --> 00:34:51,600 +في القاعة تمام؟ بعد ما أنتم طلعتوا القاعة هذه مش + +458 +00:34:51,600 --> 00:34:55,720 +هيزورها حد إلا تاني يوم، البكتيريا نكحتها هتقعد + +459 +00:34:55,720 --> 00:34:59,980 +تاني يوم، تقعد لحد تاني يوم لأنه تلات ساعات، فبالتالي + +460 +00:34:59,980 --> 00:35:07,960 +كل ما طالت فترة بقاء البكتيريا حية في البيئة كل ما + +461 +00:35:07,960 --> 00:35:11,360 +زادت إمكانية انتقالها أن تنتقل لـ host تاني يوم + +462 +00:35:19,990 --> 00:35:24,930 +It is a reservoir مخزن for certain nutrients لبعض + +463 +00:35:24,930 --> 00:35:29,530 +أنواع من المواد المغذية، الـ glycocalyx هالة توضيح + +464 +00:35:29,530 --> 00:35:33,070 +لها، بيكون قادر على أن يرتبط ببعض الأيونات والموليكولز + +465 +00:35:33,070 --> 00:35:36,870 +these can then be made available to + +466 +00:35:36,870 --> 00:35:42,190 +the cells، هي الخلية البكتيرية، هي الـ slime layer + +467 +00:35:42,190 --> 00:35:48,070 +تمام، لأن بتاعنا في بعض الأحيان ما نقدرش إحنا نتابع + +468 +00:35:48,610 --> 00:35:52,410 +حجم المعلومات اللي جاية هنا، ما أعطي محاضرة سريعاً + +469 +00:35:52,410 --> 00:35:55,490 +جداً آه؟ فإيش بنسويها هنا؟ بنسجلها + +470 +00:35:55,490 --> 00:36:01,010 +المحاضرة وبنسمع لها لاحقاً لأن أنا قاعد بكتب في + +471 +00:36:01,010 --> 00:36:04,970 +المحاضرة، قاعد بكتب وبحاول أفهم وبتفرج وكده، أكتر + +472 +00:36:04,970 --> 00:36:09,450 +من الشهر اللي بعملها، فالـ capsule ممكن في حال تدفق + +473 +00:36:09,450 --> 00:36:14,950 +كثير من الـ molecules حولها أن تلتصق فيها و + +474 +00:36:14,950 --> 00:36:19,940 +تخليها موجودة لحد ما ضغط الخلية يستوعبهم إلى الداخل + +475 +00:36:19,940 --> 00:36:25,760 +يعني مخزن خارجي .. مخزن خارجي لبعض الموليكوليات، طيب + +476 +00:36:25,760 --> 00:36:35,020 +في كمان لسه مخزن أو وظيفة خاجسة Depot for waste + +477 +00:36:35,020 --> 00:36:40,280 +product يعني برضه كمان يعني مكاب .. خلينا نقول أو + +478 +00:36:40,280 --> 00:36:44,740 +release .. slow release للـ waste product، waste + +479 +00:36:44,740 --> 00:36:47,280 +products of metabolism are executed from the cell + +480 +00:36:47,550 --> 00:36:51,290 +لأن الـ waste product بصير إخراج إليها إلى خارج + +481 +00:36:51,290 --> 00:36:55,430 +الخلية and will accumulate in the capsule، حاسفين + +482 +00:36:55,430 --> 00:36:59,910 +يهدرعكم أين؟ في الـ capsule، this wind them up and + +483 +00:36:59,910 --> 00:37:02,910 +prevent the waste from interfering with cell + +484 +00:37:02,910 --> 00:37:09,030 +metabolism، قلنا إنه الإرتباط للـ waste products + +485 +00:37:09,030 --> 00:37:15,640 +خارج الخلية بيجعلهم غير قادرين على قضية الخلية الـ + +486 +00:37:15,640 --> 00:37:18,840 +waste product، خدوا بالكم، معروف إن الـ waste بيقضي + +487 +00:37:18,840 --> 00:37:22,940 +الخلية نفسه، فارتباطهم وكأنه بنجمّعهم في مكان واحد + +488 +00:37:22,940 --> 00:37:31,340 +بنبعدهم عن مين؟ عن الـ metabolism تبع الخلية، خمس + +489 +00:37:31,340 --> 00:37:37,380 +وظائف للـ flagella عفواً، ده الـ capsule، وظائف قيّمة + +490 +00:37:37,380 --> 00:37:42,480 +جداً تجعل من الـ capsule بحق أحد الـ virulence factors + +491 +00:37:42,480 --> 00:37:47,620 +المهمة سواء كان في الـ infection كـ attachment أو + +492 +00:37:47,620 --> 00:37:54,020 +كهروب من الـ immune system زي ما قلنا لهروب + +493 +00:37:54,020 --> 00:38:00,320 +من الـ phagocytosis أو أنها تتّصل في المواد وتخزينها + +494 +00:38:00,320 --> 00:38:04,230 +لحد استخدامها أو للـ waste product كمان أيضاً عشان + +495 +00:38:04,230 --> 00:38:08,090 +أنك تتخلص من السمومية تبعك، نجي الآن لتركيب آخر برضه + +496 +00:38:08,090 --> 00:38:12,270 +من التراكيب العظيمة فيه البكتيريا واسمه flashlight + +497 +00:38:12,270 --> 00:38:17,410 +بتسمى هو بحاجة اسمه nano technology، nanotechnology + +498 +00:38:17,410 --> 00:38:21,790 +تكنولوجيا الـ nano اللي هو العربية وهي توظيف جزيئات + +499 +00:38:21,790 --> 00:38:31,220 +بمقياس nano وعمل أجهزة، الأجهزة هذه عبارة عن يعني + +500 +00:38:31,220 --> 00:38:37,160 +حجم من واحد nano لميت nano أجهزة، تخيلوا موتور كهرباء + +501 +00:38:37,160 --> 00:38:44,200 +غير مرئي الفلاجلة الفلاجلة هي عبارة عن nano motor + +502 +00:38:44,200 --> 00:38:47,760 +nano motor يعني موتور nano يعني ينطبق على التعريف + +503 +00:38:47,760 --> 00:38:50,320 +ال nano technology بس مش إحنا اللي صنعناها مين + +504 +00:38:50,320 --> 00:38:55,970 +اللي صنعه؟ رب العالمين موجود في البكتيريا nanomotor + +505 +00:38:55,970 --> 00:38:58,610 +نانو موتور هنشوف كمان شوية بس خلينا نطلع على بعض + +506 +00:38:58,610 --> 00:39:02,350 +نعرفها some bacterial species are mobile and + +507 +00:39:02,350 --> 00:39:06,770 +possesses locomotory organ locomotory يعني حركة + +508 +00:39:06,770 --> 00:39:12,130 +أمام organ يعني organelles اللي هي عضية الحركة + +509 +00:39:12,130 --> 00:39:17,740 +اسمها flagella كمان مرة some مش كل البكتيريا، يعني + +510 +00:39:17,740 --> 00:39:21,540 +خبنا كده نقول من الآن إن بعض البكتيريا متحركة و + +511 +00:39:21,540 --> 00:39:27,340 +البعض الآخر غير متحرك، لكن معظم البكتيريا المتحركة + +512 +00:39:27,340 --> 00:39:31,780 +متحركة ب flagella، بمعنى آخر أحد أهم أعضاء الحركة + +513 +00:39:31,780 --> 00:39:35,480 +في البكتيريا هو ال flagella، تظهرونك أعضاء أخرى، + +514 +00:39:35,480 --> 00:39:42,380 +نعم، هنتكلم عنها لاحقا إن شاء الله those that do not + +515 +00:39:42,380 --> 00:39:46,000 +are able to sense their environment and response + +516 +00:39:46,000 --> 00:39:49,120 +to specific chemical good stuff or toxic material + +517 +00:39:49,120 --> 00:39:54,660 +and move towards or away from them كلمة move + +518 +00:39:54,660 --> 00:40:00,780 +toward or away from a stimuli بنسميها chemotaxis + +519 +00:40:00,780 --> 00:40:05,640 +إيش يعني chemotaxis الإنتقال إلى أو الإبتعاد عن + +520 +00:40:05,640 --> 00:40:11,700 +stimuli تخيلوا لو واحد مقعد وقالته نار هبط عليه نار + +521 +00:40:11,700 --> 00:40:17,720 +وممكن يهرب منها بلاش تفطوره موجود غاد في المنطقة + +522 +00:40:17,720 --> 00:40:23,440 +ديك هل بيقدر ينتقل إذا كان معاه organ of motility + +523 +00:40:23,440 --> 00:40:30,480 +إحنا البشر نمشي نتحرك باتجاه أو بالابتعاد بكتير + +524 +00:40:30,480 --> 00:40:34,290 +غير متحركة لا تمتلك هذه الرفاهية إذا كنت أعرف من + +525 +00:40:34,290 --> 00:40:38,410 +الآن إن البكتيريا المتحركة فيها صفة إيجابية، ألا + +526 +00:40:38,410 --> 00:40:42,950 +وهي الإبتعاد أو الذهاب إلى، والانتقال فيه خير + +527 +00:40:42,950 --> 00:40:47,490 +للبكتيريا، صح أو لا، تروح على غذاء أو تبتعد عن خطر + +528 +00:40:47,490 --> 00:40:52,090 +معين الفلاجلة are embedded in the cell membrane، + +529 +00:40:52,090 --> 00:40:55,550 +يبقى منطقة الفلاجلة، من ال cell wall؟ لا، موجودة + +530 +00:40:55,550 --> 00:40:58,090 +في ال cell membrane، عارفين إيش بال cell membrane؟ + +531 +00:40:58,090 --> 00:41:03,130 +لو تذكرت وظائف ال cell membrane، حتى حد جاوبني أحد + +532 +00:41:03,130 --> 00:41:07,030 +وظائف ال cell membrane هو energy production و أنا + +533 +00:41:07,030 --> 00:41:09,970 +جاي أقول لكم الفلاجلة عبارة عن موتور الموتور + +534 +00:41:09,970 --> 00:41:13,110 +مش بده طاقة؟ فمنهم ياخد طاقة من ال cell membrane + +535 +00:41:13,110 --> 00:41:16,730 +لذلك هو embedded مغروس في ال cell membrane لكن + +536 +00:41:16,730 --> 00:41:21,150 +الفلاجلة is protruding خارج من إطار اللي هو ال + +537 +00:41:21,150 --> 00:41:23,550 +cell membrane إلى ال cell wall إلى خارج الإطار + +538 +00:41:23,550 --> 00:41:29,620 +هتشوف صورة إن شاء الله flagella consist of a number + +539 +00:41:29,620 --> 00:41:34,200 +of proteins including flagellin هو ال main protein + +540 +00:41:34,200 --> 00:41:39,020 +الأساسي لل flagella سهل اسمه flagellin طبعا زي ال + +541 +00:41:39,020 --> 00:41:41,400 +protein تبع البلاي اسمه pellin فبالتالي ما فيش + +542 +00:41:41,400 --> 00:41:45,560 +صعوبة في أن تحفظ ال flagellin هو ال protein تبع ال + +543 +00:41:45,560 --> 00:41:49,600 +flagella they move the cell by rotating with a + +544 +00:41:49,600 --> 00:41:54,080 +propeller like action وهذه النقطة بدنا نفهمها هي + +545 +00:41:54,080 --> 00:41:58,170 +الخلية وهي ال flagella الفلاجلة عبارة عن .. أنت + +546 +00:41:58,170 --> 00:42:02,770 +عارفين؟ حاجة مرمة، صوت، تمام؟ فالآن تتحرك الفلاجلة + +547 +00:42:02,770 --> 00:42:08,310 +بهذا الشكل وكأنها بتشتغل بطريقة دافعة، طريقة عبارة + +548 +00:42:08,310 --> 00:42:11,450 +عن موتور بتتحرك بهذا الشكل، فبيدفع البكتيريا إلى + +549 +00:42:11,450 --> 00:42:15,270 +الأمام، طبعا برضه هـ .. هعرض لكم المحاضرة الجاية إن + +550 +00:42:15,270 --> 00:42:17,190 +شاء الله بعض الفلاشات، إنها هتكون المحاضرة الجاية + +551 +00:42:17,190 --> 00:42:22,550 +غالبا توضيحات لل structure تبع الـ .. يعني .. يعني + +552 +00:42:22,550 --> 00:42:25,710 +كله متعلق بين المحاضرة السابقة، هعوضكم بعض + +553 +00:42:25,710 --> 00:42:28,890 +التوضيحات إن شاء الله التوضيحية اللي تزيد من فهمكم + +554 +00:42:28,890 --> 00:42:33,830 + لخلية البكتيريا، لأنه إحنا معنيين جدا جدا إن + +555 +00:42:33,830 --> 00:42:37,970 +الطالب يخرج من هذا الجزء عارف إيش يعني bacterial + +556 +00:42:37,970 --> 00:42:42,880 +cell structure، مش حافظه بس، فاهم، لأنه كثير من + +557 +00:42:42,880 --> 00:42:47,840 +القضايا المتعلقة في ال medical microbiology مرتبطة + +558 +00:42:47,840 --> 00:42:52,060 +أساسا بفهمنا لـ ال bacterial cell structure تشوفوا + +559 +00:42:52,060 --> 00:43:00,560 +الآن السرعات التقريبية لبعض الكائنات، تحفظوا تشيتا، + +560 +00:43:00,560 --> 00:43:06,320 +تشيتا هو نوع من الفهود، طبعا، وقلنا بسرعتها the + +561 +00:43:06,320 --> 00:43:11,710 +hour، 111 كيلومتر في الساعة body length per second + +562 +00:43:11,710 --> 00:43:17,710 +يعني كم قفزة في طول جسمها؟ لأ، قداش المسافة اللي + +563 +00:43:17,710 --> 00:43:23,230 +بتقطعها طول جسمها طول جسمها 25 مرة في الثانية طول + +564 +00:43:23,230 --> 00:43:28,850 +جسمها المسافة الـ human كام مرة؟ 5 و 4 طول جسمه + +565 +00:43:28,850 --> 00:43:32,390 +البكتيريا طالع للسرعة تبعها point zero zero zero + +566 +00:43:32,390 --> 00:43:37,310 +zero one five kilometer طبعا بالنسبة لحجمها + +567 +00:43:37,310 --> 00:43:41,210 +بالنسبة لحجمها هذه صورة معمولة جدا بالنسبة لحجمها + +568 +00:43:41,210 --> 00:43:44,930 +صورة معمولة جدا لإنه لاحظوا برضه أسرع من البشر + +569 +00:43:44,930 --> 00:43:51,450 +همام أسرع من الإنسان لكن حجم الإنسان بيعطيه قطع + +570 +00:43:51,450 --> 00:43:56,430 +مسافة أكبر لإنه بيبقى .. يعني العملية relative to + +571 +00:43:56,430 --> 00:44:00,690 +the size of the organism ال cheetah خمسة وعشرين مرة + +572 +00:44:00,690 --> 00:44:05,730 +طول جسمه في الثانية الواحدة الإنسان خمسة وأربع + +573 +00:44:05,730 --> 00:44:07,010 +هذه عشر مرات + +574 +00:44:30,910 --> 00:44:34,390 +هذا الشكل اللي قدامكم هو الربط وهنا ال bacterial + +575 +00:44:34,390 --> 00:44:38,070 +cell structure أو bacterial structure طالع منه + +576 +00:44:38,070 --> 00:44:41,310 +مجموعة من الفلاجلة الفلاجلة مش واحدة طلعتها أكثر من + +577 +00:44:41,310 --> 00:44:44,570 +ما إحنا مظبوط هنشوف إنّه إنّه بالفعل في بعض الخلايا + +578 +00:44:44,570 --> 00:44:47,690 +فيها بيطلع منها فلاجلة واحدة بيطلع منها عن طرفين + +579 +00:44:47,690 --> 00:44:53,570 +حوالي إنبات بيختلف من cell لآخر طلعوا لها الشكل هذا + +580 +00:44:53,570 --> 00:44:57,690 +اللي للأسف مش واضح كثير أمامنا لكن هو عبارة عن + +581 +00:44:57,690 --> 00:45:04,210 +أجزاء بتشكل اللي هو الـ .. الـ .. cell membrane + +582 +00:45:04,210 --> 00:45:09,570 +embedded motor يعني الموتور اللي مغروس فيه في ال + +583 +00:45:09,570 --> 00:45:12,770 +cell membrane وانا فيه عندي هذا الـ hook اللي هو زي + +584 +00:45:12,770 --> 00:45:16,190 +خطاف وانا فيه عندي اللي هو الـ filament يعني أكثر + +585 +00:45:16,190 --> 00:45:18,930 +.. أكثر .. أكثر من قسم بين .. بين قسم بين الـ ثلاث + +586 +00:45:18,930 --> 00:45:29,710 +أقسام نرسمهم اللي هو هذا الـ .. بصير ونسميه + +587 +00:45:29,710 --> 00:45:36,340 +basal body وبعدين الـ hook اللي هو شايفين هو ده + +588 +00:45:36,340 --> 00:45:40,800 +المنحني ده وبعدين من الـ hook بتطلع اللي هو الـ + +589 +00:45:40,800 --> 00:45:44,900 +filament الصورة + +590 +00:45:44,900 --> 00:45:47,180 +اللي شوفناها جاء وشوية عبارة عن مين؟ عن الـ + +591 +00:45:47,180 --> 00:45:50,200 +filament اللي في الصورة اللي ظهر بينما الـ hook و + +592 +00:45:50,200 --> 00:45:53,020 +الـ basil body داخلة عشان نشوفهم في الـ bacteria + +593 +00:45:53,020 --> 00:46:00,920 +عموما نتخيل الـ bacteria حجم كبير بالمقارنة مع مين؟ + +594 +00:46:01,340 --> 00:46:05,560 +مع الفلاجلة هل يمكن رؤية الفلاجلة؟ إذا إحنا قادرين + +595 +00:46:05,560 --> 00:46:08,800 +نتكلم عن البكتيريا إنّه بنشوفها تحت الـ microscope + +596 +00:46:08,800 --> 00:46:15,340 +بنات شكل شحطة بهذا الشكل هل يمكن لنا رؤية الفلاجلة + +597 +00:46:15,340 --> 00:46:19,460 +اللي طالعة منها؟ لأ، لذلك إذا قررنا صبغة الفلاجلة + +598 +00:46:19,460 --> 00:46:23,980 +يجب أن نقوم بصبغة خاصة الصبغة الخاصة هذه معتمدة + +599 +00:46:23,980 --> 00:46:33,400 +على إيش؟ على إنّه نحط صبغة قادرة على الالتصاق على + +600 +00:46:33,400 --> 00:46:36,260 +الفلاجلة specifically وبالتالي بتزيد من قطر من + +601 +00:46:36,260 --> 00:46:41,260 +الصبغة نفسها بتترسب على الفلاجلة فبتزيد من قطر + +602 +00:46:41,260 --> 00:46:45,540 +الفلاجلة وبالتالي يمكن أيضا أو بيسميها special + +603 +00:46:45,540 --> 00:46:49,960 +flagella stain أنا + +604 +00:46:49,960 --> 00:46:55,260 +قلت لكم إنّه فينا توزيع مختلف للفلاجلة لوحتنا هذه + +605 +00:46:55,260 --> 00:46:58,920 +الأخيرة في هذه المحاضرة هنتكلم على mono-trigas اللي + +606 +00:46:58,920 --> 00:47:03,460 +تلاحظه one single flagella فيه عندي amphitrigas + +607 +00:47:03,460 --> 00:47:09,960 +وهو على الطرفين تمام الـ amphitrichous على الطرفين المفروض + +608 +00:47:09,960 --> 00:47:16,160 +إنّه مش الـ .. مش بيه الـ lophotrichous اللي هو بيه الـ lophotrichous + +609 +00:47:16,160 --> 00:47:24,060 +يعني حزمة من الـ flagella at one pole الأخيرة هي + +610 +00:47:24,060 --> 00:47:28,800 +الأخيرة اللي هي الـ peritrichous around بيدي معناها around + +611 +00:47:28,800 --> 00:47:33,860 +فالفرجلة الشاشة معمولة موزعة حول كل خلية إذا بس + +612 +00:47:33,860 --> 00:47:38,160 +الاختلاف هو إنّ أنا في الصورة بأنّ الـ lophotrichous هو بيه و + +613 +00:47:38,160 --> 00:47:42,320 +الـ amphitrichous هي الـ سي لإنّ الـ amphitrichous معناها متردد أو على + +614 +00:47:42,320 --> 00:47:45,600 +الطرفين ونختم إن شاء الله بهذه اللوحة ونكمل + +615 +00:47:45,600 --> 00:47:46,400 +المحاضرة القادمة diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..05c764865449dc1586856495679553858df852bf --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ_postprocess.srt @@ -0,0 +1,2460 @@ +1 +00:00:21,890 --> 00:00:24,950 +بسم الله الرحمن الرحيم احنا اتواصفنا المحاضرة + +2 +00:00:24,950 --> 00:00:28,090 +الماضية عن تركيب من تركيب الخلية الداخلية اللي هو + +3 +00:00:28,090 --> 00:00:34,690 +ال mesosome و بدنا نتعرف على مهيط ال mesosome هو + +4 +00:00:34,690 --> 00:00:40,070 +عبارة عن invaginations نقول عنها هي بغى من مين؟ من + +5 +00:00:40,070 --> 00:00:44,210 +ال cell، من ال brain، و ال cell و ال brain فيه + +6 +00:00:44,210 --> 00:00:48,530 +وظائف عديدة، ماعرفش اتركني لها لسه، لكن هذه + +7 +00:00:48,530 --> 00:00:56,630 +الوظائفإن هو عبارة عن إنتاج الطاقة وإن هو المقابل + +8 +00:00:56,630 --> 00:01:00,330 +لـMitochondria في الـEucaryotes فلو هناك نظرية + +9 +00:01:00,330 --> 00:01:05,970 +غريبة في التطور بتقول إن الـEucaryotic cell ما + +10 +00:01:05,970 --> 00:01:10,050 +كانت قادرة على إدارة الطاقة دخلتلها بكتيريا جوا + +11 +00:01:10,050 --> 00:01:16,690 +فالـEucaryote فرحت فيها وحولتها من بكتيريا إلى + +12 +00:01:16,690 --> 00:01:22,060 +Mitochondria هيك الأسطورة تتبع التطورعموما + +13 +00:01:22,060 --> 00:01:26,600 +البيتوكندرزوم يزيد مساعة السطح لأنه extension + +14 +00:01:26,600 --> 00:01:31,560 +للcell membrane وبالتالي أي وظيفة للcell membrane + +15 +00:01:31,560 --> 00:01:35,660 +هو بيزيد هذه الوظيفة والصورة اللي بتانكو هي عبارة + +16 +00:01:35,660 --> 00:01:39,640 +عن تشبيه واضح ما بين البيتوكندرية اللي هي الكريستا + +17 +00:01:39,640 --> 00:01:41,700 +إذا فاكرين الكريستا في تركيب اللي هي الأعراف + +18 +00:01:41,700 --> 00:01:46,000 +بالمقابل مع ال invagination اللي احنا رسمناها هنا + +19 +00:01:46,000 --> 00:01:50,770 +أو هي ظاهرة هنابتزيد مساحة السطح وبتكون مكان مناسب + +20 +00:01:50,770 --> 00:01:54,710 +جدا لعملية اللي هو ال Oxidative Phosphorylation و + +21 +00:01:54,710 --> 00:02:00,470 +Other Energy Producing Pathways خلصنا + +22 +00:02:00,470 --> 00:02:04,690 +الجدار الخلوي خلصنا ال cell membrane ومتعلق بيه + +23 +00:02:04,690 --> 00:02:08,590 +كان ال mesosome لأن دخلنا إلى داخل الخلية لا يدد + +24 +00:02:08,590 --> 00:02:12,250 +بيها إلا بحر من ال cytoplasm و ال cytoplasm مكون + +25 +00:02:12,250 --> 00:02:19,240 +mainly من water ماء مذاق في بعض الأملاحو السكريات + +26 +00:02:19,240 --> 00:02:23,220 +بالاضافة إلى أنه موجود داخله نوكليك أسيت بروتينات + +27 +00:02:23,220 --> 00:02:28,080 +and other compounds الآن من المراكبات أو من + +28 +00:02:28,080 --> 00:02:30,860 +التراكيب المهم جدا في pseudoplasmia المتشهرة بشكل + +29 +00:02:30,860 --> 00:02:35,720 +كبير ليها الريبوزومز وريبوزومز أذكركم بأنها من نوع + +30 +00:02:35,720 --> 00:02:42,640 +70S 70S اللي قصرناها ال vaccine أو fifty thirty + +31 +00:02:42,640 --> 00:02:47,200 +وبنذكركوا دايما بهذا المقولة لأنه معتمدين وهي أمكن + +32 +00:02:47,200 --> 00:02:54,070 +حتىيعني مذكورة هنا إنه هي المكان المستهدف لكثير من + +33 +00:02:54,070 --> 00:02:58,610 +ال antibioticsالـ Macrolides، الـ Tetracyclines، + +34 +00:02:58,610 --> 00:03:02,130 +الجنتاميسين وعائلة كبيرة اسمها الـ Aminoglycosides + +35 +00:03:02,130 --> 00:03:07,310 +كلها بتستهدف لبروتين سينتيز إما على ال 50S أو على + +36 +00:03:07,310 --> 00:03:11,410 +ال 30S لذلك من المهم على الطلبة إنهم يكونوا عارفين + +37 +00:03:11,410 --> 00:03:16,030 +لبروتين سينتيز أساسا كيف بيتم عشان لما نيجي نشرح + +38 +00:03:16,030 --> 00:03:19,650 +كيف بنوقف لبروتين سينتيز نكون عارفين كيف أصلا + +39 +00:03:19,650 --> 00:03:24,320 +لبروتين سينتيز أساسا بيتمإن ال ribosomes موزع على + +40 +00:03:24,320 --> 00:03:28,240 +كل الخلية تعتبر المصانع الرئيسية تصنيع ال protein + +41 +00:03:28,240 --> 00:03:32,300 +في الخلية ال plasmids هي تراكيب إضافية مهولة في + +42 +00:03:32,300 --> 00:03:35,440 +بعض البكتيريا مش في كل البكتيريا بينما ال ribosome + +43 +00:03:35,440 --> 00:03:39,660 +لازم يكون في كل البكتيريا هنا قد تحتوي البكتيريا + +44 +00:03:39,660 --> 00:03:43,440 +على plasmid أو قد لا تحتوي على plasmid لكن هي + +45 +00:03:43,440 --> 00:03:48,960 +عبارة عن extra chromosomal DNA material يعني مادة + +46 +00:03:48,960 --> 00:03:54,260 +وراثية إضافيةخارج عن طاقمين الكروموزوم هتكلم معانا + +47 +00:03:54,260 --> 00:03:59,780 +شوية بتفاصيل أكتر و نعرف كيف تشتغل ال plasma ال + +48 +00:03:59,780 --> 00:04:07,320 +inclusions الحبيبات نميل نحن إلى التخزين أشياء + +49 +00:04:07,320 --> 00:04:11,710 +ضرورية لناخاصة لما تكون موجودة in abundance، يعني + +50 +00:04:11,710 --> 00:04:15,090 +بزيادة، تمام؟ في البيئة المحيطة فينا، بنحاول أنه + +51 +00:04:15,090 --> 00:04:18,790 +نخزنها، معانا عشرين دفتر، بناخد دفتر و بنخبه تسعة + +52 +00:04:18,790 --> 00:04:22,810 +عشر، عندنا أرضية معينة، بناخد جزء منها و بنخزنه + +53 +00:04:22,810 --> 00:04:27,370 +بالتلاجة أو بنخزنه كما يليق بنوع الطعام، إذا غالبا + +54 +00:04:27,370 --> 00:04:31,430 +ال inclusion هي for storage، what type of storage؟ + +55 +00:04:31,430 --> 00:04:35,870 +غالبا، غالبا energy storage، والبكتير اللي ياخده + +56 +00:04:35,870 --> 00:04:43,690 +بالكمis a very wise when it is related to energy + +57 +00:04:43,690 --> 00:04:48,490 +يعني بمعنى لو البكتيريا دخلت و هي ميتة من الجوا + +58 +00:04:48,490 --> 00:04:53,230 +starving بمعنى إنها قعدت فترة طويلة بدون غذاء و + +59 +00:04:53,230 --> 00:04:57,190 +فجأة حاطناها في nutrient rich ما بتبدأ تاكل على + +60 +00:04:57,190 --> 00:05:01,270 +طول تبتطل على البسط، هي طبعا ماتشوفش زي احنا موز و + +61 +00:05:01,270 --> 00:05:06,710 +تفاح، هي ماتشوف glucose و lactoseودهون وبروتين + +62 +00:05:06,710 --> 00:05:10,310 +وcarbohydrates فلو وردت على سبيل المثال جلوكوز + +63 +00:05:10,310 --> 00:05:15,510 +ولاكتوز ابتبدأ بالجلوكوز ليش؟ لأنه عندها انزيمات + +64 +00:05:15,510 --> 00:05:20,410 +هيك هيك منتجة للجلوكوز لو بدأت باللاكتوز مضطرة + +65 +00:05:20,410 --> 00:05:25,370 +تنتج انزيمات معينة كلمة تنتج يعني استهلاك الطاقة + +66 +00:05:25,370 --> 00:05:29,410 +فهي حكيمة جدا في استخدام الطاقة وإن كانت خلايا + +67 +00:05:29,410 --> 00:05:35,730 +صغيرة لكن هي very efficient factorsالريبوزومز زي + +68 +00:05:35,730 --> 00:05:40,830 +ما ذكرتلكوا ال complete ribosome هو 70S و70S او ال + +69 +00:05:40,830 --> 00:05:43,790 +S هي sedimentation units اللي منها Zwittberg unit + +70 +00:05:43,790 --> 00:05:48,570 +لما حصرناهم عن بعض وجدنا ال large 50 و ال small و + +71 +00:05:48,570 --> 00:05:52,350 +الاتنين بيكونوا بالفعل مفصلين و بيصيرهم assembly + +72 +00:05:52,350 --> 00:05:55,670 +مع ال mRNA و بتم عملية اللي هو ال protein + +73 +00:05:55,670 --> 00:05:57,030 +synthesis + +74 +00:05:59,500 --> 00:06:03,420 +البروتين الـ synthesis بتتم في الخلايا بواسطة اللي + +75 +00:06:03,420 --> 00:06:06,500 +هو مين الـ ribosomes they translate the + +76 +00:06:06,500 --> 00:06:09,360 +information يعني بجانب الكلام أنا هذا information + +77 +00:06:09,360 --> 00:06:12,980 +مخزنة في المادة اللي ورا فيها انا عندي information + +78 +00:06:12,980 --> 00:06:16,340 +مخزنة في الدماغ بنقلكوا إيها عبر اللسان وعبر + +79 +00:06:16,340 --> 00:06:20,580 +الحركات وعبر الصور بعض الأحيان عبر الفيديو لكن + +80 +00:06:20,580 --> 00:06:22,980 +البكتيريا ما بتتكلم لغتنا احنا اللغة اللي احنا + +81 +00:06:22,980 --> 00:06:28,980 +بنتكلم فيها بتتكلمها لغتها أبسط من ذلكلكن ملطورة + +82 +00:06:28,980 --> 00:06:31,540 +احنا اليوم مبسوطين علي حالنا معانا flash memory + +83 +00:06:31,540 --> 00:06:35,940 +مصبوح واحد صديق بيعطينا ال flash بيقولنا خد هذا + +84 +00:06:35,940 --> 00:06:39,840 +بصلاحك ال computer او بنزلك ويندوز انت مش عارف كيف + +85 +00:06:39,840 --> 00:06:42,520 +بنزل ويندوز او كيف بيشيل الفيروس من ال .. لكن + +86 +00:06:42,520 --> 00:06:47,630 +بيشيله بقطرةاللي هو ال flash memory اللي عليها + +87 +00:06:47,630 --> 00:06:52,470 +information software تمام؟ نفس الجثة لما نشوف كمان + +88 +00:06:52,470 --> 00:06:55,430 +شوية ال plasmids هنعرف كيف البكتيريا بتنقل + +89 +00:06:55,430 --> 00:06:58,490 +المعلومات من بكتيريا إلى أخرى عبر ال information + +90 +00:06:58,490 --> 00:07:01,930 +تمام؟ يعني مافيش داعي تعمل محاضرات للآخرين و + +91 +00:07:01,930 --> 00:07:06,530 +تحملهم جميلة لأ بتعطيهم قطعة من المادة الوراثية و + +92 +00:07:06,530 --> 00:07:12,070 +انتهى الموضوعإذن they translate mRNA and produces + +93 +00:07:12,070 --> 00:07:16,590 +the information على شكل البروتين البروتين هو ال + +94 +00:07:16,590 --> 00:07:20,390 +expression للمعلومات الموجودة في ال RNA لأصل + +95 +00:07:20,390 --> 00:07:26,270 +المعلومة المأخوذة أساسا من ال DNA لبلازميت أنا + +96 +00:07:26,270 --> 00:07:32,050 +حاولت التعريف أقطع قطعة صغيرة عشان انزهل المعلومات + +97 +00:07:32,050 --> 00:07:35,330 +هو البلازميت عبارة عن small circular extra + +98 +00:07:35,330 --> 00:07:41,750 +chromosomalخارج نطاق الكروموزوم double-stranded + +99 +00:07:41,750 --> 00:07:45,970 +DNA molecule في حد عنده شك أن ال DNA أصلا double + +100 +00:07:45,970 --> 00:07:51,270 +-stranded ما هو double-stranded و لأ حسيته شك مين + +101 +00:07:51,270 --> 00:07:55,490 +درس فيروسات ولا لسه محدش درس فيروسات في الفيروسات + +102 +00:07:55,490 --> 00:07:59,030 +هناك بعض الفيروسات المادة الوراثية بتكون single + +103 +00:07:59,030 --> 00:08:03,110 +-stranded DNA وبالتالي عملنا stressing يعني تأكيد + +104 +00:08:03,110 --> 00:08:06,910 +على كلمة double-stranded DNA moleculethey are + +105 +00:08:06,910 --> 00:08:11,650 +capable of self-replicate تنقسم ال .. ال .. ال .. + +106 +00:08:11,650 --> 00:08:15,550 +ال plasma independently of the chromosome يعني ال + +107 +00:08:15,550 --> 00:08:19,450 +chromosome بنقسم لحال و هي تنقسم لحال مافيش ارتباط + +108 +00:08:19,450 --> 00:08:24,110 +بينهم مافيش ارتباط بينهم هل من المعقول انه انا + +109 +00:08:24,110 --> 00:08:27,070 +المعلومات اللي عندي في الدماغ اذا بده انقلكوا + +110 +00:08:27,070 --> 00:08:31,360 +إياها اعطيكوا إياها و ابقى من غير المعلومات هذهولا + +111 +00:08:31,360 --> 00:08:35,240 +لازم اعمل نسخ عنها بعمل نسخ و بعطيكوا بحجم النسخ + +112 +00:08:35,240 --> 00:08:37,420 +اللي .. عشان انا كنت بقول انه ال .. ال .. ال + +113 +00:08:37,420 --> 00:08:41,080 +plasma هاي مثلا ال plasma عاملة replication في + +114 +00:08:41,080 --> 00:08:45,020 +اكتر من نسخة في الخلية هي واحدة اتنين تلاتة اربعة + +115 +00:08:45,020 --> 00:08:50,720 +وهذا تمثيل قسمي لمين؟ للكرموزوم يعني ان التوضيح + +116 +00:08:50,720 --> 00:08:54,960 +للكرموزوم اكبر بكتير من مين من ال plasma كمان برضه + +117 +00:08:54,960 --> 00:08:59,540 +small circular extra chromosomal double stranded + +118 +00:08:59,950 --> 00:09:07,250 +DNA capable of self-replication and contains genes + +119 +00:09:07,250 --> 00:09:14,310 +تحتوي على جينات that confer تمنح تعطي some + +120 +00:09:14,310 --> 00:09:18,890 +properties such as antibiotic resistance تخيلوا أن + +121 +00:09:18,890 --> 00:09:23,190 +البكتيريا تتبادل المعرفة في كيفية حماية نفسها من + +122 +00:09:23,190 --> 00:09:28,850 +ال antibiotic بكتيريا بطريقة ما صارت عندها المعرفة + +123 +00:09:30,060 --> 00:09:33,080 +بمقاومة ال .. ال .. ال antibiotic اسمه Benzalic + +124 +00:09:33,080 --> 00:09:37,840 +كيف؟ عن طريق إنتاج أنزين اسمه Penicillinase ال + +125 +00:09:37,840 --> 00:09:41,600 +Penicillinase بيكسر ال Penicillin فبالتالي بيصير + +126 +00:09:41,600 --> 00:09:44,100 +ال Benzaline غير فعال ضد البكتيريا وبالتالي + +127 +00:09:44,100 --> 00:09:47,260 +البكتيريا الآن protected من ال Penicillin صح ولا + +128 +00:09:47,260 --> 00:09:51,200 +مش صح؟ طيب هذه المعلومات وين مخزنة إنتاج ال + +129 +00:09:51,200 --> 00:09:54,980 +Penicillinase على ال plasma فلو نقلت البكتيريا + +130 +00:09:54,980 --> 00:09:59,870 +المقاومةالبلاسمة تبقى حال البكتيريا غير مقاومة + +131 +00:09:59,870 --> 00:10:04,990 +تصبح البكتيريا الأخرى مقاومة زي ما قلت لكوا ال + +132 +00:10:04,990 --> 00:10:10,190 +flash حطيناها في جهاز تمام أخذ من ال flash معلومة + +133 +00:10:10,190 --> 00:10:13,690 +أخذ من الجهاز معلومة كيفية مكافحة فيروس معين فيروس + +134 +00:10:13,690 --> 00:10:19,770 +حسوبي أخدناه وحطيناه في الجهاز التاني الجهاز تنكسب + +135 +00:10:19,770 --> 00:10:24,010 +إخلاء صفة أنه مقاوم لهذا الفيروسطبعا مش بس + +136 +00:10:24,010 --> 00:10:28,550 +antibiotic resistance كمان ممكن تنقل جينات دات + +137 +00:10:28,550 --> 00:10:32,190 +معلومات خاصة بإنتاج سموم على سبيل المثال يعني + +138 +00:10:32,190 --> 00:10:37,490 +بكتيريا بريقة مايلها المقدرة على إنتاج سم لما + +139 +00:10:37,490 --> 00:10:42,150 +نقلناها لبلازميل صلاة تنتج هذا السم أي مادة من + +140 +00:10:42,150 --> 00:10:48,130 +شأنها أن تجعل البكتيريا أكثر شراسة أكثر إمراضة + +141 +00:10:48,130 --> 00:10:54,210 +بيسميها virulence factor أي عامليجعل من البكتيريا + +142 +00:10:54,210 --> 00:10:59,130 +قادرة على إحداث المرض بنسميه Virulence Factor مهم + +143 +00:10:59,130 --> 00:11:03,130 +كتير المعلومة هذه لأنها حد رح نكررها مرارا وتكرار + +144 +00:11:03,130 --> 00:11:07,950 +Plasmids are not essential for the survival of the + +145 +00:11:07,950 --> 00:11:12,990 +cell بمعنى أنه مش ليس ال wall ولا ال cell membrane + +146 +00:11:12,990 --> 00:11:16,330 +ولا ال ribosomes لو مافيش Plasmids ممكن تعيش + +147 +00:11:16,330 --> 00:11:20,150 +البكتيريا بشرط أنه تعيش في ظروف ملائمة + +148 +00:11:23,860 --> 00:11:27,500 +طب هتنتقل البلازميت من خلية لخلية؟ نعم هتنتقل، + +149 +00:11:27,500 --> 00:11:31,100 +هتتكلم عن حسمة conjugation اللي هي الاقتران + +150 +00:11:31,100 --> 00:11:36,640 +الجنسي، خلية مع خلية بتبادلوا المعلومات أنا تحدثت + +151 +00:11:36,640 --> 00:11:39,920 +إن في السترات البلازم، فينا inclusion bodies، وكنا + +152 +00:11:39,920 --> 00:11:44,240 +ال inclusion bodies هي عبارة عن storage granules، + +153 +00:11:44,240 --> 00:11:48,000 +والغرض من ال storage أو الداعي لل storage هو ال + +154 +00:11:48,000 --> 00:11:52,300 +abundance، اللي هو فيه كمية كبيرة موجودة من الغذاء + +155 +00:11:52,300 --> 00:11:58,870 +في المحيططبعا تخزن هذا الغذاء الزائد لحين الحاجة + +156 +00:11:58,870 --> 00:12:01,930 +تمام؟ فيه granules بيسموها storage granules أو بعض + +157 +00:12:01,930 --> 00:12:05,970 +الأحيان inclusions طبعا الحديث عن هذا الموضوع + +158 +00:12:05,970 --> 00:12:08,670 +بيختلف لما اتكلم في ال medical microbial بيختلف عن + +159 +00:12:08,670 --> 00:12:10,990 +.. لما اتكلم عن ال .. ال .. ال environmental فى + +160 +00:12:10,990 --> 00:12:15,730 +بكتيريا عصيمتال بتخزن المغناطسات بيسموها + +161 +00:12:15,730 --> 00:12:20,390 +magnetosomes بتخزن فى حالها فبصير بكتيريا مغناطسية + +162 +00:12:20,390 --> 00:12:24,430 +بيسموها magnetotactic bacteriaالمجنيدوتاكتك + +163 +00:12:24,430 --> 00:12:28,850 +بكتيريا تستخدم الميغناطيسات هذه في الحفاظ على + +164 +00:12:28,850 --> 00:12:33,210 +نفسها في مستوى معين في المياه مثلا في مياه البراكا + +165 +00:12:33,210 --> 00:12:36,970 +أو المياه العادمة تمام؟ بدل ما تطفو على السطح أو + +166 +00:12:36,970 --> 00:12:40,730 +تستقر في القاع بترتب الميغناطيسات بحاجة إنها تكون + +167 +00:12:40,730 --> 00:12:44,130 +في مستوى معين هذا المستوى المعين ملائم إلا من + +168 +00:12:44,130 --> 00:12:50,310 +ناحية الأكسجين يعني وكأنه وكأنه وسيلة anchoring + +169 +00:12:50,310 --> 00:12:55,620 +مرسابترسي نفسها في مكان معين مفضل لها الظروف فهناك + +170 +00:12:55,620 --> 00:13:00,600 +الكثير من ال inclusion granules المتنوعة لكن + +171 +00:13:00,600 --> 00:13:04,780 +معظمها الغرض الأساسي منها إيه يا بنات ال storage + +172 +00:13:04,780 --> 00:13:10,900 +وال storage تبقى إيه؟ تبقى energy molecules هذه + +173 +00:13:10,900 --> 00:13:13,920 +بعض الصور ال electron microscope بتوضح لنا ما فينا + +174 +00:13:13,920 --> 00:13:17,640 +اللي هو inclusion bodies في كثير من أنواع من + +175 +00:13:17,640 --> 00:13:22,260 +البكتير أو granulesأحنا اتفقنا إن أصلا + +176 +00:13:22,260 --> 00:13:25,380 +البروكاريوتس لا تحتوي على نوكلياس فاحنا حاطينا + +177 +00:13:25,380 --> 00:13:29,000 +عنوان نوكلياس عشان ننفيه فقط لغة نقولكوا إنه + +178 +00:13:29,000 --> 00:13:33,640 +lacking nuclear membrane absence of nuclei اللي هي + +179 +00:13:33,640 --> 00:13:38,320 +بقينا نسميها نواة و نوية هه hence known as .. لذلك + +180 +00:13:38,320 --> 00:13:43,020 +النواة في البروكاريوتس تسمى nucleic material أو + +181 +00:13:43,020 --> 00:13:47,240 +نوكليايد انسوا الدم الأخير هذا هو كان مش موجود + +182 +00:13:50,230 --> 00:13:53,990 +الان الصورة اللى أمامكوا هذه، طلعولي عليها، حد + +183 +00:13:53,990 --> 00:14:01,050 +عنده تخيل إيش هذه الصورة، شايفينها أو مش شايفينها؟ + +184 +00:14:01,050 --> 00:14:04,650 +شجرة؟ + +185 +00:14:04,650 --> 00:14:13,770 +عفا مثلا خالد تفضلي، هذه ال chromosome تعلق شريشة + +186 +00:14:13,770 --> 00:14:20,690 +أكوليةلو فرضناه بيمشي + +187 +00:14:20,690 --> 00:14:24,530 +معانا من أول الجامعة لآخر الجامعة والبكتيريا ليست + +188 +00:14:24,530 --> 00:14:30,350 +طولها واحد مايكروم هذا الحجم و هذا التعقيد كله + +189 +00:14:30,350 --> 00:14:33,650 +موجود في واحد مايكروم + +190 +00:14:37,250 --> 00:14:40,410 +اللي أنا قلتلكوا هو single و circular و لما + +191 +00:14:40,410 --> 00:14:44,390 +البكتيريا بده تنتج .. بده تنتج انزين معين ابتعد + +192 +00:14:44,390 --> 00:14:47,590 +تأمر للجين الوحيد اللي موجود لدى الانزين من كل + +193 +00:14:47,590 --> 00:14:52,490 +هالخربة طيارة انه يشتغل small but terrible small + +194 +00:14:52,490 --> 00:14:58,210 +but efficient يعني صغيرة لكن رهيبة صغيرة لكن فعالة + +195 +00:14:58,210 --> 00:15:03,390 +إياكم أن تنظروا للبكتيريا على إنها يعني مخلوق يعني + +196 +00:15:03,390 --> 00:15:09,730 +في السلسلة و انخفضالبكتيريا عندها .. قلنا قبل شوية + +197 +00:15:09,730 --> 00:15:12,970 +في ال energy أشطر من إطلاع الصحراء وبتعرف تنجي + +198 +00:15:12,970 --> 00:15:16,830 +أكلها أكتر من ما نختاره، بتعرف كيف تهرب من المواد + +199 +00:15:16,830 --> 00:15:21,450 +السامة، بتعرف كيف تتكثر بشكل فعال، كيف تخزن، يعني + +200 +00:15:21,450 --> 00:15:25,230 +كل المهارات اللي بيمتلكها البشر، تمام؟ ومن ضمنها + +201 +00:15:25,230 --> 00:15:31,890 +التعليم، تتعلم البكتيريا بشكل جيد من بعضها البعض، + +202 +00:15:31,890 --> 00:15:36,830 +single ال chromosome, circular and compactedعشان + +203 +00:15:36,830 --> 00:15:39,530 +هيكن هذا اللي طوله من الأول جامعة لأخر جامعة + +204 +00:15:39,530 --> 00:15:46,510 +بينضبط كله في واحد مايكن نيجي لتركيب مهم جدا اسمه + +205 +00:15:46,510 --> 00:15:49,330 +ال capsule أو الإصلاح الغلاف و أنا بتمنى عليكم + +206 +00:15:49,330 --> 00:15:53,450 +انكوا يعني اللوحة شوية تبقى قدوة عنها و تنتبهوا + +207 +00:15:53,450 --> 00:15:59,990 +لكلام اللي بتحكي الغلاف + +208 +00:15:59,990 --> 00:16:04,290 +الخارجي اللي أول غلاف بحيط بال cell wall لما طلعنا + +209 +00:16:04,290 --> 00:16:12,400 +برا هو ال capsuleهذه الـ bacteria هذا + +210 +00:16:12,400 --> 00:16:15,580 +الـ structure اللي ورا الـ cell wall بنسميها الـ + +211 +00:16:15,580 --> 00:16:20,180 +capsule العلماء احترفوا تسميته الصحيح يعني لو + +212 +00:16:20,180 --> 00:16:22,980 +طلعتوا على الكتب بتلاقيه capsule، glycocalyx، + +213 +00:16:22,980 --> 00:16:26,660 +slime layer، S layer، micro capsule طب الإختلافات + +214 +00:16:26,660 --> 00:16:30,080 +هذه إيش كانت سببها؟ الإختلافات هذه كانت سببها + +215 +00:16:30,080 --> 00:16:34,680 +شغلتين رئيسيتين ال thickness سماكة + +216 +00:16:36,130 --> 00:16:45,270 +الطبقة وانتظامها السماكة ماسميها thickness و ال + +217 +00:16:45,270 --> 00:16:49,170 +organization اللي + +218 +00:16:49,170 --> 00:16:55,150 +هو تنظيم الكتشوف لحظه هذا regular منتظم لو تخيلنا + +219 +00:16:55,150 --> 00:17:01,850 +تركيب آخر عامل زي هى بنفس ال thickness هذا + +220 +00:17:01,850 --> 00:17:09,210 +منتظم مش منتظمفهل نسمي هذا نفس اسم الكابسول ولا ما + +221 +00:17:09,210 --> 00:17:13,130 +نسموهاش؟ فقال صار في عنا شبه اتفاق، يمكن مش مذكور + +222 +00:17:13,130 --> 00:17:17,750 +قدرنا هنا، حاجة بيسموها glycocalyx أي تركيب، أي + +223 +00:17:17,750 --> 00:17:22,910 +تركيب بينطبق على صفات الكابسول هذه اللي هنا بيسموه + +224 +00:17:22,910 --> 00:17:25,530 +glycocalyx، لكن بيسموه glycocalyx إلى أربعة أصلا، + +225 +00:17:25,530 --> 00:17:29,890 +خلينا نكتبهم على الصرف، glyco + +226 +00:17:35,680 --> 00:17:40,820 +الجليجوكاليكس قد يكون واحد + +227 +00:17:40,820 --> 00:17:45,620 +من أربعة capsule micro + +228 +00:17:45,620 --> 00:17:51,420 +capsule slime + +229 +00:17:51,420 --> 00:17:55,460 +layer و + +230 +00:17:55,460 --> 00:17:58,960 +slime على لزج يعني طبقة لزجة و هي كله أصلا لزجة + +231 +00:17:58,960 --> 00:18:03,000 +هنشوف كمان شوية الوضعات تبعتها و S layer + +232 +00:18:06,040 --> 00:18:13,140 +الان مال اتشابه ومال اختلاف بين الأربع هدول واضح + +233 +00:18:13,140 --> 00:18:16,240 +كابسول وكابسول في بينهم علاقة و layer و layer برضه + +234 +00:18:16,240 --> 00:18:19,480 +فيهم علاقة لعلاقة اللي بينهم هدول لإنه تنتين + +235 +00:18:19,480 --> 00:18:24,320 +organized بينما + +236 +00:18:24,320 --> 00:18:26,680 +ال slime layer لعلاقة اللي بينهم انه none + +237 +00:18:26,680 --> 00:18:34,080 +organized فبدا هذا الشيء ال common ايش الاختلاف؟ + +238 +00:18:35,450 --> 00:18:44,370 +الـ Thickness يعني + +239 +00:18:44,370 --> 00:18:48,750 +الكابسول organized والمايكرو كابسول organized واضح + +240 +00:18:48,750 --> 00:18:52,330 +أن كلمة كابسول هي الأضخم فال thickness يبقى عالي + +241 +00:18:52,330 --> 00:18:55,390 +المايكرو كابسول organized بس ايه ال thickness صغير + +242 +00:18:55,390 --> 00:19:00,670 +ال slime layer مش organized لكن thick ال S layer + +243 +00:19:00,670 --> 00:19:05,060 +مش organized لكن thinو هي الفروقات بين الأربعة، + +244 +00:19:05,060 --> 00:19:10,440 +إذا لو سمعتوا في يوم من الأيام مصطلح glycocalyx، + +245 +00:19:10,440 --> 00:19:14,020 +capsule و لا micro capsule و لا S layer و لا slam + +246 +00:19:14,020 --> 00:19:18,360 +layer، كلهم في النهاية هم اللي أنتوا بتعرفوهم باسم + +247 +00:19:18,360 --> 00:19:22,800 +مين؟ باسم capsule، باسم capsule، لكن معنين إنه + +248 +00:19:22,800 --> 00:19:26,700 +نقلنه، كتير من الكتب صارت تتكلم عنهم بضرر مختلفة و + +249 +00:19:26,700 --> 00:19:29,920 +متنوعة، فكويس إن أنتوا عارفين هذه المعلومة + +250 +00:19:32,340 --> 00:19:36,920 +ما هي .. اه مما اتكون الكابسول هذه نقطة كتير مهمة + +251 +00:19:36,920 --> 00:19:47,240 +ايش التركيب الكيميائي للكابسول مكتوب من اللوحة من + +252 +00:19:47,240 --> 00:19:52,220 +polysaccharide لكن هناك exception هناك exception + +253 +00:19:52,220 --> 00:19:58,600 +هي ال polysaccharide رأيتها؟ when however certain + +254 +00:19:58,600 --> 00:20:03,080 +bacilli they are composed of polypeptideوأجيبلكم + +255 +00:20:03,080 --> 00:20:07,960 +مثال صارخ على هذه البكتيريا عشان تحفظوا اسمها، كم + +256 +00:20:07,960 --> 00:20:12,000 +اسم البكتيريا اللي تحفظينها اليوم؟ ولا واحدة؟ + +257 +00:20:22,290 --> 00:20:27,490 +Bacillus anthracis وحي المسبب لجامرة الخبيثة جامرة + +258 +00:20:27,490 --> 00:20:32,390 +الخبيثة مرض خطير جدا أمريكا أتهمت العراق في حرب + +259 +00:20:32,390 --> 00:20:36,930 +الخليج الأول والثانية على أنه تمتلك مئات الأطنان + +260 +00:20:36,930 --> 00:20:38,790 +من هذه البكتيريا وأنه هتستخدمها في السلاح + +261 +00:20:38,790 --> 00:20:44,450 +البيولوجي فبكتيريا خطيرة الكابسول تبعتها تتكون من + +262 +00:20:44,450 --> 00:20:48,870 +polypeptide خلافا لمعظم أنواع البكتيريا المتكونة + +263 +00:20:48,870 --> 00:20:55,750 +من مين قلنا من polyهتذكركم الأربعة شريشيا كولاي، + +264 +00:20:55,750 --> 00:20:59,270 +Staphylococcus aureus مايكوبلازما اللي ملاقش cell + +265 +00:20:59,270 --> 00:21:03,010 +wall تمام من بارح وهي البكتيريا اللي احنا بتكلم + +266 +00:21:03,010 --> 00:21:11,590 +عنها البسيلاس أنتراسيس أنصحكم بالمتابعة أولا بأول + +267 +00:21:11,590 --> 00:21:17,490 +حتى لا تتركن المعلومات اليوم بعض + +268 +00:21:17,490 --> 00:21:22,950 +الصور لل capsule تبوا معاياهذه صورة electron + +269 +00:21:22,950 --> 00:21:27,350 +microscope تظهر انها كابسول الكابسولة انا اظهرت + +270 +00:21:27,350 --> 00:21:32,510 +انها irregular صح هذه irregular مرة هنا سميكة و + +271 +00:21:32,510 --> 00:21:38,190 +مرة هنا رقيقة فهذه نسميها اما slim layer او s + +272 +00:21:38,190 --> 00:21:42,990 +layer حسب المقارنة في ال thickness بينما هذه رحظة + +273 +00:21:42,990 --> 00:21:50,270 +regular لكن thin فبسموها micro caps الصورتين هدولة + +274 +00:21:50,560 --> 00:21:53,640 +هم عبارة عن صورتين هتشوفوهم في المعمل ان شاء الله + +275 +00:21:53,640 --> 00:21:56,760 +لما تشتغلوا ال capsule stain يعني في احنا في + +276 +00:21:56,760 --> 00:22:02,100 +المعمل جزئية متعلقة بسباقة الكابسول فهنشوف + +277 +00:22:02,100 --> 00:22:07,200 +البكتيريا عبارة عن حاجة hallow او dark و حواليها + +278 +00:22:07,200 --> 00:22:11,540 +منطقة hallow يعني نفس البكتيريا هتكون dark لأنه + +279 +00:22:11,540 --> 00:22:14,820 +بنستخدم ما يسمى بال negative stain ال negative + +280 +00:22:14,820 --> 00:22:20,140 +stain يعنيبنصبغ الخلفية وبنصبغش الخلية نفسها + +281 +00:22:20,140 --> 00:22:23,320 +الخلفية تبعت البكتيريا بتصبغ بينما البكتيريا + +282 +00:22:23,320 --> 00:22:28,500 +لاتصبغ لأن حضرة ال capsule على الرغم من انها + +283 +00:22:28,500 --> 00:22:36,340 +virulence factor مهم جدا اللي انها delicateرقيقة، + +284 +00:22:36,340 --> 00:22:40,940 +هشة، سهل لو عملناها بالمعالجات فكل gram is 10؟ + +285 +00:22:40,940 --> 00:22:44,620 +crystal violet و iodine و Ethanol و بعدين سفرانين، + +286 +00:22:44,620 --> 00:22:48,840 +هذه معالجات صعبة على البكتيريا، لو الكابسول موجودة + +287 +00:22:48,840 --> 00:22:52,880 +ستفقد الكابسول، فنعالجها بطريقة معينة هنشوفها في + +288 +00:22:52,880 --> 00:22:58,880 +المعبنة الشعرية الآن نيجي لل functions of the + +289 +00:22:58,880 --> 00:23:02,920 +capsule، و ركزوا كويس، لإن ال functions هذه كتير + +290 +00:23:02,920 --> 00:23:09,730 +مهمة، رقم واحد، attachmentهنشوف function عكسها + +291 +00:23:09,730 --> 00:23:14,190 +تماما للـ capsule عكس الـ attachment هروب من الـ + +292 +00:23:14,190 --> 00:23:19,170 +attachment مظبوط ان الـ attachment التصاق بنا + +293 +00:23:19,170 --> 00:23:23,490 +تخيلوا معي التخيل هذا الـ urinary tract بتبتدأ من + +294 +00:23:23,490 --> 00:23:27,550 +وين؟ من ال kidneys مظبوط و بتروح الحالب و الحالب + +295 +00:23:27,550 --> 00:23:30,150 +بيصف فيه اللي هو ال .. ال .. ال .. ال urinary + +296 +00:23:30,150 --> 00:23:33,410 +bladder و بعدين ال .. ال .. ال .. الخارج لو + +297 +00:23:33,410 --> 00:23:38,080 +بكتيريا جاءت تتسلق في الـ ureterقطرة بول اعتقد + +298 +00:23:38,080 --> 00:23:41,860 +قطرة البول عارفين حاجة من قطرة طبعا قطرة البول + +299 +00:23:41,860 --> 00:23:45,740 +جداش حجب من المقارنة مع البكتيريا كبير القطرة اللي + +300 +00:23:45,740 --> 00:23:50,900 +ضخمة صح البكتيريا صغيرة خالص فلو نزلت عليها قطرة + +301 +00:23:50,900 --> 00:23:55,940 +تشبيه زي ما واحد جاعت تحت شلالة نياجرة طبعا واحد + +302 +00:23:55,940 --> 00:24:01,060 +واحد منها بيصير بترد الحشرة فبالتالي عملية سقوط + +303 +00:24:01,060 --> 00:24:06,850 +البول من أعلى بتطرد البكتيريا إلى أسفلإلا إذا كان + +304 +00:24:06,850 --> 00:24:12,290 +البكتيريا عندها المقض على إيش؟ على التاجب يبقى لن + +305 +00:24:12,290 --> 00:24:16,750 +يحدث urinary tract infection التهابات مسالك بولية + +306 +00:24:16,750 --> 00:24:21,490 +إلا إلا دي أنواع من البكتيريا تمتلك المقض على مين؟ + +307 +00:24:21,490 --> 00:24:26,170 +على التاجب يبقى ال virulence factor essential + +308 +00:24:26,170 --> 00:24:30,950 +أساسي مهم جدا جدا ان نقول البكتيريا عندها المقض + +309 +00:24:30,950 --> 00:24:33,270 +على الالتصاف طيب + +310 +00:24:36,380 --> 00:24:39,380 +لما ندرس diagnostic microbiology أو حتى في ال .. + +311 +00:24:39,380 --> 00:24:42,500 +في ال medical microbiology ان شاء الله، هنتعرف على + +312 +00:24:42,500 --> 00:24:46,760 +بعض المصطلحات منها infection، مصطلح infection، + +313 +00:24:46,760 --> 00:24:49,600 +هتلاقوا ان ال infection عبارة عن successful + +314 +00:24:49,600 --> 00:24:54,180 +colonization، طب ايش colonization؟ successful + +315 +00:24:54,180 --> 00:24:59,000 +existence في مكان لم تكن مولد فيه بكتير سابقاالـ + +316 +00:24:59,000 --> 00:25:01,800 +Successful هذا مع الـ Successful هذا بيقدّوا إلى + +317 +00:25:01,800 --> 00:25:05,720 +أو قد يقدّوا إلى disease لكن لن يكون هناك success + +318 +00:25:05,720 --> 00:25:09,860 +إلا ده كان فيه إمكانية attachment البكتيريا عشان + +319 +00:25:09,860 --> 00:25:14,640 +تتغذى لازم تلتصق إلا إذا كان في بيئة سائل، بيئة + +320 +00:25:14,640 --> 00:25:19,640 +جسد بتلتصق على خلايا عشان تتغذى على هذه الخلايا + +321 +00:25:19,640 --> 00:25:25,110 +لأن التصاق عملية مهمةthese structures are thought + +322 +00:25:25,110 --> 00:25:28,110 +to be to help cell attached to their target + +323 +00:25:28,110 --> 00:25:31,870 +environment target environment يعني و هذا و هذا + +324 +00:25:31,870 --> 00:25:34,210 +اخدوا بأكوا ال attachment بيكون عبارة عن receptor + +325 +00:25:34,210 --> 00:25:38,430 +receptor يعني مش التصاق لإنه ال .. لإنه ال .. ال + +326 +00:25:38,430 --> 00:25:43,490 +.. ال capsule لذجة لأ الاتصاق بيكون عبر ايه؟ + +327 +00:25:43,490 --> 00:25:51,090 +receptor يعني فيها receptor للأنزل مثال عليهابصحى + +328 +00:25:51,090 --> 00:25:56,990 +اسم جديد mutants produces a slime layer in the + +329 +00:25:56,990 --> 00:26:02,510 +presence of sucrose اللي بتشوفوا أصغار أطفال ماراش + +330 +00:26:02,510 --> 00:26:06,410 +على المدرسة و أسنانه كلها مسوسة مصبوط تسأل أمه + +331 +00:26:06,410 --> 00:26:10,390 +بيقولك هذه ما هو بيأكلش غير حلو الحلو رايش انه حلو + +332 +00:26:10,390 --> 00:26:16,890 +سكر و السكر اللي بنعرفه سكروز فالسكروز اللي هو + +333 +00:26:16,890 --> 00:26:20,460 +السكرلو البكتيريا اللي اسمها streptococcus mutants + +334 +00:26:20,460 --> 00:26:25,820 +وهي تعيش بالفن بالمناسبة حضرتها في وجود ال .. في + +335 +00:26:25,820 --> 00:26:28,480 +وجود ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +336 +00:26:28,480 --> 00:26:28,500 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +337 +00:26:28,500 --> 00:26:28,900 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +338 +00:26:28,900 --> 00:26:29,320 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +339 +00:26:29,320 --> 00:26:29,860 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +340 +00:26:29,860 --> 00:26:30,000 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +341 +00:26:30,000 --> 00:26:30,400 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +342 +00:26:30,400 --> 00:26:30,620 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +343 +00:26:30,620 --> 00:26:31,020 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +344 +00:26:31,020 --> 00:26:32,880 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +345 +00:26:32,880 --> 00:26:38,720 +ال .. ال .. ال .. ال .. ال .. + +346 +00:26:38,720 --> 00:26:39,720 +ال .. + +347 +00:26:48,350 --> 00:26:52,010 +and many bacteria can stick to tooth surfaces and + +348 +00:26:52,010 --> 00:26:52,830 +cause decay + +349 +00:27:00,170 --> 00:27:03,990 +لما بتتكاثر البكتيريا و بتتغذى على ال carbohydrate + +350 +00:27:03,990 --> 00:27:09,830 +بتنتج acid، ال acid بيتفاعل مع ال calcium اللي + +351 +00:27:09,830 --> 00:27:13,930 +موجود في الأسنان، فبياخد ال calcium من الأسنان، + +352 +00:27:13,930 --> 00:27:16,110 +لما ياخد ال calcium من الأسنان هو مين اللي بيخلي + +353 +00:27:16,110 --> 00:27:20,310 +الأسنان قوية و صلبة؟ ال calcium، وبالتالي تبدأ هنا + +354 +00:27:20,310 --> 00:27:24,990 +التشوهات أو التسوّس اللي احنا بيسميه تسوّس الأسنان + +355 +00:27:24,990 --> 00:27:28,790 +أو ما يسمى ب dental caries، dental caries، تسوّس + +356 +00:27:28,790 --> 00:27:34,830 +الأسنانخدنا مثال على مين؟ على الـ streptococcus + +357 +00:27:34,830 --> 00:27:39,050 +mutants في مثال تاني على حسب الـ Vibrio cholera و + +358 +00:27:39,050 --> 00:27:42,070 +ال Vibrio cholera من اسمها بتسبب مرض الكوليرة + +359 +00:27:42,070 --> 00:27:47,250 +إسهال شديد جدا يقتل الأطفال في كثير من البلدان في + +360 +00:27:47,250 --> 00:27:50,950 +العالم و عندنا في فلسطين حدثت مجموعة من الأوبياء + +361 +00:27:50,950 --> 00:27:54,890 +إلى الكوليرة لكن ربنا سلم يعني ما كان فيه نسبة + +362 +00:27:54,890 --> 00:28:00,870 +انتشار واسع also produces a glycocalyxwhich helps + +363 +00:28:00,870 --> 00:28:04,830 +to attach to the intestinal villi in the host يعني + +364 +00:28:04,830 --> 00:28:09,690 +بأهلها ترتبط في ال intestinal villi داخل العقل + +365 +00:28:09,690 --> 00:28:15,310 +وإلا وإلا مع الإسهال كل ال vibro هتطلع برا، صح + +366 +00:28:15,310 --> 00:28:19,590 +ولا؟ وبتلبط لل disease، وخد بالك الإسهال أصلا هو + +367 +00:28:19,590 --> 00:28:23,970 +ميكانيزم من الجسم دفاعية للطرد، لكن في كثير من + +368 +00:28:23,970 --> 00:28:27,010 +الأحيان بتزيد الردد الفعل من عينا لدرجة أنها + +369 +00:28:27,010 --> 00:28:31,190 +تستهلك، تستهلكال .. ال .. ال electrolytes و الماء + +370 +00:28:31,190 --> 00:28:35,630 +فبموت .. بموت الشخص مش من البكتيرا و الصوم تبعها و + +371 +00:28:35,630 --> 00:28:38,590 +أنها مرض الفعل تبعاتنا الطبيعية ما نسميه الجفاف و + +372 +00:28:38,590 --> 00:28:41,110 +.. و .. و اختلال ال electrolyte اللي بيسموه + +373 +00:28:41,110 --> 00:28:45,730 +electrolyte imbalance يبقى اتفاقنا على function + +374 +00:28:45,730 --> 00:28:49,770 +أخر واحد اسمها attachment نيجي بالتانية protection + +375 +00:28:49,770 --> 00:28:53,890 +from phagocytic engulfing كل كوب ماد غسلته بصابون + +376 +00:28:53,890 --> 00:28:58,630 +ايداكه بصابون مش سائل صابون قطعوكلكوا أكيد في + +377 +00:28:58,630 --> 00:29:02,310 +حياتكوا مرة عليكم بتكونوا أول من يفتح الصابون + +378 +00:29:02,310 --> 00:29:05,330 +بينكوا بتشيل ورد عنها صح ولا لأ؟ إذا بتشيل ورد + +379 +00:29:05,330 --> 00:29:10,390 +بتمسكيها بتجع منك، لكن أولادكوا هتتبلل، وأول + +380 +00:29:10,390 --> 00:29:13,930 +حركتين اللي الصابون نقطة من هنا أو قفزت هنا، + +381 +00:29:13,930 --> 00:29:19,170 +مظبوط؟ ليش؟ لأنها أصبحت لذية تخيلوا البكتيريا، هي + +382 +00:29:19,170 --> 00:29:22,830 +البكتيريا، وعليها الطبقة اللذية، بتجي فجوه ساعت؟ + +383 +00:29:23,010 --> 00:29:26,930 +بتلتقمها، ما بتلتقمها، بتضغط عليها اللي هي هارب + +384 +00:29:26,930 --> 00:29:31,230 +منها، فأهم وضع .. عكس ال attachment صح؟ ال + +385 +00:29:31,230 --> 00:29:33,350 +attachment كانت عبارة عن يجب أنها receptor to + +386 +00:29:33,350 --> 00:29:37,690 +receptor، هنا لإنها لازجة هاربت من الفاجوسيتوس، و + +387 +00:29:37,690 --> 00:29:43,010 +أنا هنا مضطر أجف و أوضح نقطة أساسية في تعامل + +388 +00:29:43,010 --> 00:29:45,590 +الإنسان أو البكتيريا مع الإنسان أو الحيوان أو مع + +389 +00:29:45,590 --> 00:29:46,290 +الhost يعني + +390 +00:29:50,840 --> 00:29:55,920 +أنتم الأن equal من ناحية الشراسة، تمام؟ لاتمتلكون + +391 +00:29:55,920 --> 00:29:58,920 +عوامل شراسة غير أسنانكم، وفي المدارس وانتم صاحبين + +392 +00:29:58,920 --> 00:30:03,700 +الشعب، مظبوط؟ الآن عوامل الشراسة، نفترض أننا + +393 +00:30:03,700 --> 00:30:05,740 +أعطينا زميلتكم مسدس، لأن انتوا عارفين أننا نبيئة + +394 +00:30:05,740 --> 00:30:12,500 +نبيئة المسدس هذا، الآن المسدس هذا أعطاها قدرة على + +395 +00:30:12,500 --> 00:30:15,960 +إحداث ضرر للآخرين، نسمي المسدس عامل شراسة، + +396 +00:30:15,960 --> 00:30:20,520 +أعطاناها سكين،برضه أعطناها عامل قوة، عامل شراسة، + +397 +00:30:20,520 --> 00:30:25,040 +سميناها violence factor، أعطناها واقع لرصاص، يبقى + +398 +00:30:25,040 --> 00:30:29,400 +أعطاناها إيش الآن؟ عامل شراسة للحماية من المسدس، + +399 +00:30:29,400 --> 00:30:31,840 +وبالتالي برضه عامل شراسة، أعطتها قوة، أصلا + +400 +00:30:31,840 --> 00:30:35,860 +اتهاجموا يا مش خايفة، أمان؟ اللي أنا دقان .. اللي + +401 +00:30:35,860 --> 00:30:40,080 +أنا وضحته إنه في أسلحة كانت هجومية، مسدس، سكين، + +402 +00:30:40,080 --> 00:30:44,150 +وكان فيه إيش الآن أنا؟ أسلحة دفاعيةالحرب بيننا + +403 +00:30:44,150 --> 00:30:49,930 +وبين البكتيريا بهذه البساطة هي تمتلك أسلحة هجومية + +404 +00:30:49,930 --> 00:30:54,410 +وأسلحة دفاعية ونحن كذلك نمتلك أسلحة هجومية والدليل + +405 +00:30:54,410 --> 00:30:58,430 +على ذلك أننا الفاجو والسايتس والماكروفاج ابتلتقم + +406 +00:30:58,430 --> 00:31:02,870 +وابتقتل صح؟ هي عندها الكابسول اللي تهرب عندها + +407 +00:31:02,870 --> 00:31:06,410 +الكابسول اللي تهرب عندها التكسين اللي تقتل عندها + +408 +00:31:06,410 --> 00:31:09,790 +أسلحة هجومية تكسين وعندها أسلحة دفاعية مثلا زي + +409 +00:31:09,790 --> 00:31:15,060 +اللي هو الكابسولو سينا في الحرب فيه تمويه و خداع + +410 +00:31:15,060 --> 00:31:19,840 +البكتيريا هناك أنواع منها رائعة جدا في الخداع + +411 +00:31:19,840 --> 00:31:26,540 +disguise يعني ماكرة في الهروب من جهاز المناع زي ما + +412 +00:31:26,540 --> 00:31:32,820 +كان في بعض الاجتياحات سير في دخول من بيت لبيت دون + +413 +00:31:32,820 --> 00:31:35,680 +الخروج للشارع كان يهدموا البيت و يخرجوا للبيت + +414 +00:31:35,680 --> 00:31:39,350 +الآخر لماذا؟ لأن في مقاومين في الشارعفلو خرجنا من + +415 +00:31:39,350 --> 00:31:43,870 +الشارع في عرضة هناك أنواع من البكتيريا تنتقل من + +416 +00:31:43,870 --> 00:31:48,790 +خلية إلى خلية دون الخروج منها تمام؟ بدون تدمير + +417 +00:31:48,790 --> 00:31:54,450 +الخلية الأولى كمان يعني اختراق جزء بسيط و تستطيع + +418 +00:31:54,450 --> 00:31:57,270 +.. هذا هروب من جهاز المنع و ليس هروب .. هروب من + +419 +00:31:57,270 --> 00:32:02,230 +جهاز المنع إذا بدنا نفهم أن عملية ال pathogenesis + +420 +00:32:02,230 --> 00:32:06,990 +في البكتيريا هي عمليةwar مفتوحة حرب مفتوحة بين + +421 +00:32:06,990 --> 00:32:10,750 +الطلافين جهاز المناعة و .. و .. واخدوا بالك و احنا + +422 +00:32:10,750 --> 00:32:16,170 +دايما غالبين، دايما فايزين، متى بنهزم لما نشهر ان + +423 +00:32:16,170 --> 00:32:21,570 +احنا مرضنا؟ بنقول ان هزمنا، اما الان المعرك شغالة + +424 +00:32:21,570 --> 00:32:26,220 +في فمكوا، في أمعاكوا، في الجلد تبعكواشغال المعرك + +425 +00:32:26,220 --> 00:32:30,160 +لأنه أسلحة و عندهم أسلحة و طيبهم بيشتغلوا إحنا ما + +426 +00:32:30,160 --> 00:32:33,560 +بيشغلوش بيد المعرك إلا عندما نمرض و نكون في هذه + +427 +00:32:33,560 --> 00:32:36,900 +الحالة البكتيريا أو الفيروس قد فازة على جهاز + +428 +00:32:36,900 --> 00:32:41,200 +المنحى إذا اتفقنا على أنه فإننا ايش لغاية؟ فإننا + +429 +00:32:41,200 --> 00:32:44,080 +viral infectio اسمه capsule لإنه بيهرب من مين؟ من + +430 +00:32:44,080 --> 00:32:46,700 +الفاجو و سايد أوسسا ال viral infectio هو باب ال + +431 +00:32:46,700 --> 00:32:50,540 +attachment صح؟ بدون attachment صعبة البكتيريا تنجح + +432 +00:32:50,540 --> 00:32:56,050 +تظل في مكانها للطبيعيمن أشهر الميكروبات اللي بتدل + +433 +00:32:56,050 --> 00:32:58,990 +عليها القضية على الأمنية حاجة اسمها Streptococcus + +434 +00:32:58,990 --> 00:33:02,650 +pneumonia ومن اسمها pneumonia تعمل اتهاب نيومونيا + +435 +00:33:02,650 --> 00:33:06,330 +تركزوا معايا في هذه الدزية في صنفين من هذه + +436 +00:33:06,330 --> 00:33:11,110 +البكتيريا صنف له كابسول وصنف مالوش كابسول لما + +437 +00:33:11,110 --> 00:33:17,150 +حقنهم في فارين اللي إلها كابسول قتلت الفار واللي + +438 +00:33:17,150 --> 00:33:21,290 +مالهاش كابسول هو كأنه ماحقناش الفار بحاجة عاش + +439 +00:33:21,290 --> 00:33:25,620 +الفار، تمام؟يبجى إيش كان الفرق بين اتنين؟ ورود + +440 +00:33:25,620 --> 00:33:29,080 +الكابسول يبجى الكابسول كانت مسئولة في هذه + +441 +00:33:29,080 --> 00:33:32,280 +البكتيريا عن إنها تكون pathogenic أو non + +442 +00:33:32,280 --> 00:33:38,820 +pathogenic هذا مثال صارخ على أهمية الكابسول في ال + +443 +00:33:38,820 --> 00:33:43,200 +virus هذا + +444 +00:33:43,200 --> 00:33:46,140 +الجملة نفسه بيننا اللي فوق مافيش فيها أي إذن + +445 +00:33:46,140 --> 00:33:52,560 +function تلاتة resistance to dryيعني فين انت مقاوم + +446 +00:33:52,560 --> 00:33:55,100 +لمين؟ للجفاف ركزوا معايا في هذا الـ Disease إيه + +447 +00:33:55,100 --> 00:33:58,740 +بقى الآن إحنا لما Stripped Coccy pneumonia وان + +448 +00:33:58,740 --> 00:34:03,180 +موجودة في ال lungs بعتنا عامة انها Disease لما + +449 +00:34:03,180 --> 00:34:10,400 +بنكح مش بنطلع مع الكحة البكتيريا بنشوفها في البيئة + +450 +00:34:10,400 --> 00:34:15,080 +لو البكتيريا جفت تمام الجفاف أحد عوام القتل + +451 +00:34:15,080 --> 00:34:18,760 +للبكتيريا بنجففها بنموتها نشيل منها الميه بنموتها + +452 +00:34:20,540 --> 00:34:26,080 +فوجود capsule يجعل من تجفيف البكتيريا أمر ليس + +453 +00:34:26,080 --> 00:34:31,640 +بالسهل وبالتالي يعني بتوفر برضه حماية للبكتيريا في + +454 +00:34:31,640 --> 00:34:36,920 +البيئة كل ما طولت في البيئة كل ما زاد فرصتها + +455 +00:34:36,920 --> 00:34:42,140 +لانتقال لأشخاص آخرين صح ولا لأ؟ يعني نفترض أنه + +456 +00:34:42,140 --> 00:34:47,560 +البكتيريا قدرتها على البقاعة تلات ساعات أنا كحيت + +457 +00:34:47,560 --> 00:34:51,600 +في القاعة تمام؟ بعد ما انت طلعتو القاعة هذه مش + +458 +00:34:51,600 --> 00:34:55,720 +هيزورها حد الا تاني يوم البكتيريا نكحتها هتقعد + +459 +00:34:55,720 --> 00:34:59,980 +تاني يوم تقعد اهل تاني يوم لأنه تلت ساعة فبالتالي + +460 +00:34:59,980 --> 00:35:07,960 +كل ما طالت فترة بقاء البكتيريا حية في البيئة كل ما + +461 +00:35:07,960 --> 00:35:11,360 +زاد إمكانية الجنة انها تنتقل لhost تاني يوم + +462 +00:35:19,990 --> 00:35:24,930 +It is a reservoir مخزن for certain nutrients لبعض + +463 +00:35:24,930 --> 00:35:29,530 +أنواع من المواد المغذية الـ glycocalyx هاله توضيح + +464 +00:35:29,530 --> 00:35:33,070 +لها بونا يكون قادر على أن يرتبط ببعض الأيونات و + +465 +00:35:33,070 --> 00:35:36,870 +المونيكيولز these can then be made available to + +466 +00:35:36,870 --> 00:35:42,190 +the cells هاي الخلية البكتيرية هاي ال slime layer + +467 +00:35:42,190 --> 00:35:48,070 +تمام لأن بتاعنا في بعض الأحيان مينقدرش احنا نتابع + +468 +00:35:48,610 --> 00:35:52,410 +حجم المعلومات اللي جايه هنا ماعطي محاضرة سريعين + +469 +00:35:52,410 --> 00:35:55,490 +جدا اه؟ فاشي بنتجه ايش بنسويها هنا؟ بنسجلها + +470 +00:35:55,490 --> 00:36:01,010 +المحاضرة و بنسمع لها لاحقا لإن انا قاعد بكتب في + +471 +00:36:01,010 --> 00:36:04,970 +المحاضرة قاعد بكتب و بحاول أفهم و بتفرج و كده أكتر + +472 +00:36:04,970 --> 00:36:09,450 +من الشهر اللي بعملها فال capsule ممكن في حال تدفق + +473 +00:36:09,450 --> 00:36:14,950 +كثير من ال molecules حولها انها تلتصق فيها و + +474 +00:36:14,950 --> 00:36:19,940 +تخليها موجودة لحينفضعوط الخلية تستوعبهم إلى الداخل + +475 +00:36:19,940 --> 00:36:25,760 +يعني مخزن خارجي .. مخزن خارجي لبعض الموليكيات طيب + +476 +00:36:25,760 --> 00:36:35,020 +في كمان لسه مخزن او وظيفة خاجسة Depot for waste + +477 +00:36:35,020 --> 00:36:40,280 +product يعني برضه كمان يعني مكاب .. خلينا نقول او + +478 +00:36:40,280 --> 00:36:44,740 +release .. slow release لل waste product waste + +479 +00:36:44,740 --> 00:36:47,280 +products of metabolism are executed from the cell + +480 +00:36:47,550 --> 00:36:51,290 +لأن ال waste product بصير إخراج إليها إلى خارج + +481 +00:36:51,290 --> 00:36:55,430 +الخلية and will accumulate in the capsule حاسف + +482 +00:36:55,430 --> 00:36:59,910 +يهدرعكوا أين؟ في ال capsule this wind them up and + +483 +00:36:59,910 --> 00:37:02,910 +prevent the waste from interfering with cell + +484 +00:37:02,910 --> 00:37:09,030 +metabolism قلنا إنه الإرتباط لل waste products + +485 +00:37:09,030 --> 00:37:15,640 +خارج الخليةبيجعلهم غير قادرين على قضية الخلية ال + +486 +00:37:15,640 --> 00:37:18,840 +waste product خدوا بالك معروف إن ال waste بيقضي + +487 +00:37:18,840 --> 00:37:22,940 +الخلية نفسه فارتباطهم وكأنه بنجمحهم في مكان واحد + +488 +00:37:22,940 --> 00:37:31,340 +بنبعدهم عن مين؟ عن ال metabolism تبع الخلية خمس + +489 +00:37:31,340 --> 00:37:37,380 +وظائف ل ال flagella عفوا، ده الكابسول وظائف قريعة + +490 +00:37:37,380 --> 00:37:42,480 +جدا تجعل من الكابسول بحق أحدال virus factors + +491 +00:37:42,480 --> 00:37:47,620 +المهمة سواء كان في ال infection كattachment أو + +492 +00:37:47,620 --> 00:37:54,020 +كالهروب من ال immune system زي ما قلنا لهروب + +493 +00:37:54,020 --> 00:38:00,320 +من ال phagocytosis أو انها تتسق في المواد وتخزينها + +494 +00:38:00,320 --> 00:38:04,230 +لحين استخدامهاأو لل waste product كمان أيضا عشان + +495 +00:38:04,230 --> 00:38:08,090 +انك تتخلص من السمنية تبعك نجي الان لتركيب اخر برضه + +496 +00:38:08,090 --> 00:38:12,270 +من التراكيب العظيمة فيه البكتيريا واسمه flashlight + +497 +00:38:12,270 --> 00:38:17,410 +بتسمى هو بحاجة اسمه nano technology nanotechnology + +498 +00:38:17,410 --> 00:38:21,790 +تكنولوجيا ال nano اللي هو العربية وهي توظيف جزيئات + +499 +00:38:21,790 --> 00:38:31,220 +بمقياس nano وعمل اجهزة الاجهزة هذه عبارة عنيعني + +500 +00:38:31,220 --> 00:38:37,160 +حجم من واحد nano لميت nano أجهزة تخيلوا موتور كهرة + +501 +00:38:37,160 --> 00:38:44,200 +غير مرئي الفلاجلة الفلاجلة هي عبارة عن nano motor + +502 +00:38:44,200 --> 00:38:47,760 +nano motor يعني موتور nano يعني بينطبق عالي تعريف + +503 +00:38:47,760 --> 00:38:50,320 +ال nano technology بس مش احنا اللي صنعناها مين + +504 +00:38:50,320 --> 00:38:55,970 +اللي صنعه؟ رب العالمموجود في البكتيريا nanomotor + +505 +00:38:55,970 --> 00:38:58,610 +نانو موتور هنشوف كمان شوية بس خلينا نطلع على بعض + +506 +00:38:58,610 --> 00:39:02,350 +نعرفها some bacterial species are mobile and + +507 +00:39:02,350 --> 00:39:06,770 +possesses locomotory organ locomotory يعني حركة + +508 +00:39:06,770 --> 00:39:12,130 +أمام organ يعني organelles اللي هي عضية الحركة + +509 +00:39:12,130 --> 00:39:17,740 +اسمها flagellaكمان مرة some مش كل البكتيريا، يعني + +510 +00:39:17,740 --> 00:39:21,540 +خبنا كده نقول من الآن إن بعض البكتيريا متحرك و + +511 +00:39:21,540 --> 00:39:27,340 +البعض الآخر غير متحرك، لكن معظم البكتيريا المتحركة + +512 +00:39:27,340 --> 00:39:31,780 +متحركة ب flagella، بمعنى آخر أحد أهم أعضاء الحركة + +513 +00:39:31,780 --> 00:39:35,480 +في البكتيريا هو ال flagella، تظهرونك أعضاء أخرى، + +514 +00:39:35,480 --> 00:39:42,380 +نعم، هتورقلها لاحقا إن شاء اللهthose that do not + +515 +00:39:42,380 --> 00:39:46,000 +are able to taste their environment and response + +516 +00:39:46,000 --> 00:39:49,120 +to specific chemical good stuff or toxic material + +517 +00:39:49,120 --> 00:39:54,660 +and move towards or away from them كلمة move + +518 +00:39:54,660 --> 00:40:00,780 +toward or away from a stimuli بنسميها chemotaxis + +519 +00:40:00,780 --> 00:40:05,640 +إيش يعني chemotaxis الإنتذاب إلى أو الإبتعاد عن + +520 +00:40:05,640 --> 00:40:11,700 +stimuliتخيلوا لو واحد مقعد وقالته نار هبط عليه نار + +521 +00:40:11,700 --> 00:40:17,720 +وممكنه يهرب منها بلاش تفطوره موجود غاد في المنطقة + +522 +00:40:17,720 --> 00:40:23,440 +ديك هل بيقدر ينتقل لإذا كان معاه organ of motility + +523 +00:40:23,440 --> 00:40:30,480 +احنا البشر نمشي نتحرك باتجاه أو بالابتعاد بكتير + +524 +00:40:30,480 --> 00:40:34,290 +غير متحركة لاتمتلك هذه الرفاهيةإذا بكنت أعرف من + +525 +00:40:34,290 --> 00:40:38,410 +الآن إن البكتيريا المتحركة فيها صفة إيجابية، ألا + +526 +00:40:38,410 --> 00:40:42,950 +وهي الإبتعاد أو الذهاب إلى، والتنتين فيهم خير + +527 +00:40:42,950 --> 00:40:47,490 +للبكتيريا، صح أو لا، تروح على غذاء أو تبتعد عن خطر + +528 +00:40:47,490 --> 00:40:52,090 +معين الفلاجلة are embedded in the cell membrane، + +529 +00:40:52,090 --> 00:40:55,550 +يبقى منطقة الفلاجلة، من ال cell wall؟ لأ، مورودة + +530 +00:40:55,550 --> 00:40:58,090 +في ال cell membrane، عرفين إيش بال cell membrane؟ + +531 +00:40:58,090 --> 00:41:03,130 +لو تذكرت وظائف ال cell membrane، حتى حد جاوبونيأحد + +532 +00:41:03,130 --> 00:41:07,030 +وظائف ال cell membrane هو energy production و انا + +533 +00:41:07,030 --> 00:41:09,970 +جاي ابقى بقولكوا الفلاجلة عبارة عن موتور الموتور + +534 +00:41:09,970 --> 00:41:13,110 +مش بده طاقة فمنهم ياخد طاقة من ال cell membrane + +535 +00:41:13,110 --> 00:41:16,730 +لذلك هو embedded مغروس في ال cell membrane لكن + +536 +00:41:16,730 --> 00:41:21,150 +الفلاجلة is protruding خارج من إطار اللي هو ال + +537 +00:41:21,150 --> 00:41:23,550 +cell membrane إلى ال cell wall إلى خارج الإطار + +538 +00:41:23,550 --> 00:41:29,620 +هتشوف صورة إن شاء اللهflagella consist of a number + +539 +00:41:29,620 --> 00:41:34,200 +of proteins including flagellin هو ال main protein + +540 +00:41:34,200 --> 00:41:39,020 +الأساسي لل flagella سهل اسمه flagellin طبعا زي ال + +541 +00:41:39,020 --> 00:41:41,400 +protein تبع البلاي اسمه pellin فبالتالي مافيش + +542 +00:41:41,400 --> 00:41:45,560 +صعوبة في ان تحفظ ال flagellin هو ال protein تبع ال + +543 +00:41:45,560 --> 00:41:49,600 +flagella they move the cell by rotating with a + +544 +00:41:49,600 --> 00:41:54,080 +propeller like action وهذه النقطة بدنا نفهمها هي + +545 +00:41:54,080 --> 00:41:58,170 +الخلية وهي ال flagellaالفلاجلة عبارة عن .. انت + +546 +00:41:58,170 --> 00:42:02,770 +عارفين؟ حاجة مرمة، صوت، تمام؟ فالآن تتحرك الفلاجلة + +547 +00:42:02,770 --> 00:42:08,310 +بهذا الشكل وكأنها بتشتغل بطريقة دافعة، طريقة عبارة + +548 +00:42:08,310 --> 00:42:11,450 +عن موتور بتتحرك بهذا الشكل، فبيدفع البكتيريا إلى + +549 +00:42:11,450 --> 00:42:15,270 +الأمام، طبعا برضه ه .. هعرضلكم المحاضرة الجاية ان + +550 +00:42:15,270 --> 00:42:17,190 +شاء الله بعض الفلاشات، انها هتكون المحاضرة الجاية + +551 +00:42:17,190 --> 00:42:22,550 +غالباتوضيحات لل structure تبع ال .. يعني .. يعني + +552 +00:42:22,550 --> 00:42:25,710 +كله متعلق بين المحاضرة السابقة، هعوضكوا بعض + +553 +00:42:25,710 --> 00:42:28,890 +التلاشات ان شاء الله التوضيحية اللي تزيد من فهمكم + +554 +00:42:28,890 --> 00:42:33,830 +لخلية البكتيريا، لأنه احنا معنيين جدا جدا ان + +555 +00:42:33,830 --> 00:42:37,970 +الطالب يخرج من هذا الجزء عارف ايش يعني bacterial + +556 +00:42:37,970 --> 00:42:42,880 +cell structure، مش حافظه بس، فاهم، لأنهكثير من + +557 +00:42:42,880 --> 00:42:47,840 +القضايا المتعلقة في ال medical microbiology مرتبطة + +558 +00:42:47,840 --> 00:42:52,060 +أساسا بفهمنا ل ال bacterial cell structure تشوفوا + +559 +00:42:52,060 --> 00:43:00,560 +الآن السرعات التقريبية لبعض الكائنات، تحفظ تشيتا، + +560 +00:43:00,560 --> 00:43:06,320 +تشيتا هو نوع من فهود، طبعا، وقلنا بسرعتها the + +561 +00:43:06,320 --> 00:43:11,710 +hour، 111 كيلومتر للساعةbody length per second + +562 +00:43:11,710 --> 00:43:17,710 +يعني أكم قفزة في طول جسمها لأ قداش المسافة اللى + +563 +00:43:17,710 --> 00:43:23,230 +بتقطعها طول جسمها طول جسمها 25 مرة في الثانية طول + +564 +00:43:23,230 --> 00:43:28,850 +جسمها المسافة ال human كام مرة 5 و 4 طول جسمه + +565 +00:43:28,850 --> 00:43:32,390 +البكتيريا طالع للسرعة تبعتها point zero zero zero + +566 +00:43:32,390 --> 00:43:37,310 +zero one five kilometer طبعا بالنسبة لحجمها + +567 +00:43:37,310 --> 00:43:41,210 +بالنسبة لحجمهاهذه صورة عمهولة جدا بالنسبة لحجمها + +568 +00:43:41,210 --> 00:43:44,930 +صورة عمهولة جدا لإنه لاحظوا برضه أسرع من البشر + +569 +00:43:44,930 --> 00:43:51,450 +همام أسرع من الإنسان لكن حجم الإنسان بيعطيه قطع + +570 +00:43:51,450 --> 00:43:56,430 +مسافة أكبر لإنه بيبقى .. يعني العملية relative to + +571 +00:43:56,430 --> 00:44:00,690 +the size of the organism ال cheetah خمسة عشرين مرة + +572 +00:44:00,690 --> 00:44:05,730 +أسلب جسمه في الثانية الواحدة الإنسان خمسة وأربع + +573 +00:44:05,730 --> 00:44:07,010 +هذه عشر مرة + +574 +00:44:30,910 --> 00:44:34,390 +هذا الشكل اللي قدامكوا هو الربوط وهنا ال bacterial + +575 +00:44:34,390 --> 00:44:38,070 +cell structure او bacterial structure طالع منه + +576 +00:44:38,070 --> 00:44:41,310 +مجموعة من الفرجلة الفرجلة مش واحدة طلعتها اكتر من + +577 +00:44:41,310 --> 00:44:44,570 +ما احنا مظبوط هنشوف انه انه بالفعل في بعض الخلايا + +578 +00:44:44,570 --> 00:44:47,690 +فيها بيطلع منها فرجلة واحد بيطلع منها عن طرفين + +579 +00:44:47,690 --> 00:44:53,570 +حوالي انبت بيختلف من cell لاخر طلعوا لها الشكل هذا + +580 +00:44:53,570 --> 00:44:57,690 +اللي للأسف مش واضح كتير أمامنا لكن هو عبارة عن + +581 +00:44:57,690 --> 00:45:04,210 +أقراس بتشكل اللي هوال .. ال .. cell membrane + +582 +00:45:04,210 --> 00:45:09,570 +embedded motor يعني الموتور اللي مغروس فيه في ال + +583 +00:45:09,570 --> 00:45:12,770 +cell membrane وانا فيه عندي هذا ال hook اللي هو زي + +584 +00:45:12,770 --> 00:45:16,190 +خطاف وانا فيه عندي اللي هو ال filament يعني أكتر + +585 +00:45:16,190 --> 00:45:18,930 +.. أكتر .. أكتر من قسم بين .. بين قسم بين ال ثلاث + +586 +00:45:18,930 --> 00:45:29,710 +أقسام نرسمهم اللي هو هذا ال .. بصير ونسميه + +587 +00:45:29,710 --> 00:45:36,340 +nasalbody و بعدين ال hook اللى هو شايفين هو ده + +588 +00:45:36,340 --> 00:45:40,800 +المنحني دماغ و بعدين من ال hook بتطلع اللى هو ال + +589 +00:45:40,800 --> 00:45:44,900 +filament الصورة + +590 +00:45:44,900 --> 00:45:47,180 +اللى شوفناها جاء و شوية عبارة عن مين عن ال + +591 +00:45:47,180 --> 00:45:50,200 +filament اللى فى الصورة اللى ظهر بينما ال hook و + +592 +00:45:50,200 --> 00:45:53,020 +ال basil body دجالة عشان نشوفهم فى ال bacteria + +593 +00:45:53,020 --> 00:46:00,920 +عموما نتخيل ال bacteria حجم كبير بالمقارنة مع مين + +594 +00:46:01,340 --> 00:46:05,560 +مع الفلاجلة هل يمكن رؤية الفلاجلة إذا احنا قادرين + +595 +00:46:05,560 --> 00:46:08,800 +نتكلم عن البكتيريا إنه بنشوفها تحت ال microscope + +596 +00:46:08,800 --> 00:46:15,340 +بنات شكل شحطة بهذا الشكل هل يمكن لنا رؤية الفلاجلة + +597 +00:46:15,340 --> 00:46:19,460 +اللي طالعة منها لأ لذلك إذا قررنا صباقة الفلاجلة + +598 +00:46:19,460 --> 00:46:23,980 +يجب أن نقوم بصباقة خاصة الصباقة الخاصة هذه معتمدة + +599 +00:46:23,980 --> 00:46:33,400 +على إيش على إنه نحط صبرة قادرة على الالترصب على + +600 +00:46:33,400 --> 00:46:36,260 +الفلاجلة specifically وبالتالي بتزيد من قطر من + +601 +00:46:36,260 --> 00:46:41,260 +الصبغة نفسها بتترصب على الفلاجلة فبتزيد من قطر + +602 +00:46:41,260 --> 00:46:45,540 +الفلاجلة وبالتالي يمكن ايضا او بيسميها special + +603 +00:46:45,540 --> 00:46:49,960 +flagella state انا + +604 +00:46:49,960 --> 00:46:55,260 +قولتلكوا انه فينا توزيع مختلف للفلاجلة لوحتنا هذه + +605 +00:46:55,260 --> 00:46:58,920 +الأخيرة في هذه المحاضرةهنتكلم على mono-trigas اللي + +606 +00:46:58,920 --> 00:47:03,460 +تلاحظه one single flagella فيه عندي amphitrigas + +607 +00:47:03,460 --> 00:47:09,960 +وهو على الطرفين تمام الانفي على الطرفين المفروض + +608 +00:47:09,960 --> 00:47:16,160 +انه مش ال .. مش بيه ال loafo اللي هو بيه loafo + +609 +00:47:16,160 --> 00:47:24,060 +يعني حزمة من ال flagella at one pole الاخيرة هي + +610 +00:47:24,060 --> 00:47:28,800 +الاخيرة اللي هي ال periodAround بيدي معناها around + +611 +00:47:28,800 --> 00:47:33,860 +فالفرجلة الاشاشة معمولة موزعة حول كل تلخلية إذا بس + +612 +00:47:33,860 --> 00:47:38,160 +الاختلاف هو إن أنا في الصورة بإن ال loaf هو بي و + +613 +00:47:38,160 --> 00:47:42,320 +الانفي هي ال سي لإن الانفي معناها متردد أو على + +614 +00:47:42,320 --> 00:47:45,600 +الطرفين ونختم إن شاء الله بهذه اللوحة ونكمل + +615 +00:47:45,600 --> 00:47:46,400 +المحاضرة القادمة + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..05c764865449dc1586856495679553858df852bf --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/odJSCUX8GZQ_raw.srt @@ -0,0 +1,2460 @@ +1 +00:00:21,890 --> 00:00:24,950 +بسم الله الرحمن الرحيم احنا اتواصفنا المحاضرة + +2 +00:00:24,950 --> 00:00:28,090 +الماضية عن تركيب من تركيب الخلية الداخلية اللي هو + +3 +00:00:28,090 --> 00:00:34,690 +ال mesosome و بدنا نتعرف على مهيط ال mesosome هو + +4 +00:00:34,690 --> 00:00:40,070 +عبارة عن invaginations نقول عنها هي بغى من مين؟ من + +5 +00:00:40,070 --> 00:00:44,210 +ال cell، من ال brain، و ال cell و ال brain فيه + +6 +00:00:44,210 --> 00:00:48,530 +وظائف عديدة، ماعرفش اتركني لها لسه، لكن هذه + +7 +00:00:48,530 --> 00:00:56,630 +الوظائفإن هو عبارة عن إنتاج الطاقة وإن هو المقابل + +8 +00:00:56,630 --> 00:01:00,330 +لـMitochondria في الـEucaryotes فلو هناك نظرية + +9 +00:01:00,330 --> 00:01:05,970 +غريبة في التطور بتقول إن الـEucaryotic cell ما + +10 +00:01:05,970 --> 00:01:10,050 +كانت قادرة على إدارة الطاقة دخلتلها بكتيريا جوا + +11 +00:01:10,050 --> 00:01:16,690 +فالـEucaryote فرحت فيها وحولتها من بكتيريا إلى + +12 +00:01:16,690 --> 00:01:22,060 +Mitochondria هيك الأسطورة تتبع التطورعموما + +13 +00:01:22,060 --> 00:01:26,600 +البيتوكندرزوم يزيد مساعة السطح لأنه extension + +14 +00:01:26,600 --> 00:01:31,560 +للcell membrane وبالتالي أي وظيفة للcell membrane + +15 +00:01:31,560 --> 00:01:35,660 +هو بيزيد هذه الوظيفة والصورة اللي بتانكو هي عبارة + +16 +00:01:35,660 --> 00:01:39,640 +عن تشبيه واضح ما بين البيتوكندرية اللي هي الكريستا + +17 +00:01:39,640 --> 00:01:41,700 +إذا فاكرين الكريستا في تركيب اللي هي الأعراف + +18 +00:01:41,700 --> 00:01:46,000 +بالمقابل مع ال invagination اللي احنا رسمناها هنا + +19 +00:01:46,000 --> 00:01:50,770 +أو هي ظاهرة هنابتزيد مساحة السطح وبتكون مكان مناسب + +20 +00:01:50,770 --> 00:01:54,710 +جدا لعملية اللي هو ال Oxidative Phosphorylation و + +21 +00:01:54,710 --> 00:02:00,470 +Other Energy Producing Pathways خلصنا + +22 +00:02:00,470 --> 00:02:04,690 +الجدار الخلوي خلصنا ال cell membrane ومتعلق بيه + +23 +00:02:04,690 --> 00:02:08,590 +كان ال mesosome لأن دخلنا إلى داخل الخلية لا يدد + +24 +00:02:08,590 --> 00:02:12,250 +بيها إلا بحر من ال cytoplasm و ال cytoplasm مكون + +25 +00:02:12,250 --> 00:02:19,240 +mainly من water ماء مذاق في بعض الأملاحو السكريات + +26 +00:02:19,240 --> 00:02:23,220 +بالاضافة إلى أنه موجود داخله نوكليك أسيت بروتينات + +27 +00:02:23,220 --> 00:02:28,080 +and other compounds الآن من المراكبات أو من + +28 +00:02:28,080 --> 00:02:30,860 +التراكيب المهم جدا في pseudoplasmia المتشهرة بشكل + +29 +00:02:30,860 --> 00:02:35,720 +كبير ليها الريبوزومز وريبوزومز أذكركم بأنها من نوع + +30 +00:02:35,720 --> 00:02:42,640 +70S 70S اللي قصرناها ال vaccine أو fifty thirty + +31 +00:02:42,640 --> 00:02:47,200 +وبنذكركوا دايما بهذا المقولة لأنه معتمدين وهي أمكن + +32 +00:02:47,200 --> 00:02:54,070 +حتىيعني مذكورة هنا إنه هي المكان المستهدف لكثير من + +33 +00:02:54,070 --> 00:02:58,610 +ال antibioticsالـ Macrolides، الـ Tetracyclines، + +34 +00:02:58,610 --> 00:03:02,130 +الجنتاميسين وعائلة كبيرة اسمها الـ Aminoglycosides + +35 +00:03:02,130 --> 00:03:07,310 +كلها بتستهدف لبروتين سينتيز إما على ال 50S أو على + +36 +00:03:07,310 --> 00:03:11,410 +ال 30S لذلك من المهم على الطلبة إنهم يكونوا عارفين + +37 +00:03:11,410 --> 00:03:16,030 +لبروتين سينتيز أساسا كيف بيتم عشان لما نيجي نشرح + +38 +00:03:16,030 --> 00:03:19,650 +كيف بنوقف لبروتين سينتيز نكون عارفين كيف أصلا + +39 +00:03:19,650 --> 00:03:24,320 +لبروتين سينتيز أساسا بيتمإن ال ribosomes موزع على + +40 +00:03:24,320 --> 00:03:28,240 +كل الخلية تعتبر المصانع الرئيسية تصنيع ال protein + +41 +00:03:28,240 --> 00:03:32,300 +في الخلية ال plasmids هي تراكيب إضافية مهولة في + +42 +00:03:32,300 --> 00:03:35,440 +بعض البكتيريا مش في كل البكتيريا بينما ال ribosome + +43 +00:03:35,440 --> 00:03:39,660 +لازم يكون في كل البكتيريا هنا قد تحتوي البكتيريا + +44 +00:03:39,660 --> 00:03:43,440 +على plasmid أو قد لا تحتوي على plasmid لكن هي + +45 +00:03:43,440 --> 00:03:48,960 +عبارة عن extra chromosomal DNA material يعني مادة + +46 +00:03:48,960 --> 00:03:54,260 +وراثية إضافيةخارج عن طاقمين الكروموزوم هتكلم معانا + +47 +00:03:54,260 --> 00:03:59,780 +شوية بتفاصيل أكتر و نعرف كيف تشتغل ال plasma ال + +48 +00:03:59,780 --> 00:04:07,320 +inclusions الحبيبات نميل نحن إلى التخزين أشياء + +49 +00:04:07,320 --> 00:04:11,710 +ضرورية لناخاصة لما تكون موجودة in abundance، يعني + +50 +00:04:11,710 --> 00:04:15,090 +بزيادة، تمام؟ في البيئة المحيطة فينا، بنحاول أنه + +51 +00:04:15,090 --> 00:04:18,790 +نخزنها، معانا عشرين دفتر، بناخد دفتر و بنخبه تسعة + +52 +00:04:18,790 --> 00:04:22,810 +عشر، عندنا أرضية معينة، بناخد جزء منها و بنخزنه + +53 +00:04:22,810 --> 00:04:27,370 +بالتلاجة أو بنخزنه كما يليق بنوع الطعام، إذا غالبا + +54 +00:04:27,370 --> 00:04:31,430 +ال inclusion هي for storage، what type of storage؟ + +55 +00:04:31,430 --> 00:04:35,870 +غالبا، غالبا energy storage، والبكتير اللي ياخده + +56 +00:04:35,870 --> 00:04:43,690 +بالكمis a very wise when it is related to energy + +57 +00:04:43,690 --> 00:04:48,490 +يعني بمعنى لو البكتيريا دخلت و هي ميتة من الجوا + +58 +00:04:48,490 --> 00:04:53,230 +starving بمعنى إنها قعدت فترة طويلة بدون غذاء و + +59 +00:04:53,230 --> 00:04:57,190 +فجأة حاطناها في nutrient rich ما بتبدأ تاكل على + +60 +00:04:57,190 --> 00:05:01,270 +طول تبتطل على البسط، هي طبعا ماتشوفش زي احنا موز و + +61 +00:05:01,270 --> 00:05:06,710 +تفاح، هي ماتشوف glucose و lactoseودهون وبروتين + +62 +00:05:06,710 --> 00:05:10,310 +وcarbohydrates فلو وردت على سبيل المثال جلوكوز + +63 +00:05:10,310 --> 00:05:15,510 +ولاكتوز ابتبدأ بالجلوكوز ليش؟ لأنه عندها انزيمات + +64 +00:05:15,510 --> 00:05:20,410 +هيك هيك منتجة للجلوكوز لو بدأت باللاكتوز مضطرة + +65 +00:05:20,410 --> 00:05:25,370 +تنتج انزيمات معينة كلمة تنتج يعني استهلاك الطاقة + +66 +00:05:25,370 --> 00:05:29,410 +فهي حكيمة جدا في استخدام الطاقة وإن كانت خلايا + +67 +00:05:29,410 --> 00:05:35,730 +صغيرة لكن هي very efficient factorsالريبوزومز زي + +68 +00:05:35,730 --> 00:05:40,830 +ما ذكرتلكوا ال complete ribosome هو 70S و70S او ال + +69 +00:05:40,830 --> 00:05:43,790 +S هي sedimentation units اللي منها Zwittberg unit + +70 +00:05:43,790 --> 00:05:48,570 +لما حصرناهم عن بعض وجدنا ال large 50 و ال small و + +71 +00:05:48,570 --> 00:05:52,350 +الاتنين بيكونوا بالفعل مفصلين و بيصيرهم assembly + +72 +00:05:52,350 --> 00:05:55,670 +مع ال mRNA و بتم عملية اللي هو ال protein + +73 +00:05:55,670 --> 00:05:57,030 +synthesis + +74 +00:05:59,500 --> 00:06:03,420 +البروتين الـ synthesis بتتم في الخلايا بواسطة اللي + +75 +00:06:03,420 --> 00:06:06,500 +هو مين الـ ribosomes they translate the + +76 +00:06:06,500 --> 00:06:09,360 +information يعني بجانب الكلام أنا هذا information + +77 +00:06:09,360 --> 00:06:12,980 +مخزنة في المادة اللي ورا فيها انا عندي information + +78 +00:06:12,980 --> 00:06:16,340 +مخزنة في الدماغ بنقلكوا إيها عبر اللسان وعبر + +79 +00:06:16,340 --> 00:06:20,580 +الحركات وعبر الصور بعض الأحيان عبر الفيديو لكن + +80 +00:06:20,580 --> 00:06:22,980 +البكتيريا ما بتتكلم لغتنا احنا اللغة اللي احنا + +81 +00:06:22,980 --> 00:06:28,980 +بنتكلم فيها بتتكلمها لغتها أبسط من ذلكلكن ملطورة + +82 +00:06:28,980 --> 00:06:31,540 +احنا اليوم مبسوطين علي حالنا معانا flash memory + +83 +00:06:31,540 --> 00:06:35,940 +مصبوح واحد صديق بيعطينا ال flash بيقولنا خد هذا + +84 +00:06:35,940 --> 00:06:39,840 +بصلاحك ال computer او بنزلك ويندوز انت مش عارف كيف + +85 +00:06:39,840 --> 00:06:42,520 +بنزل ويندوز او كيف بيشيل الفيروس من ال .. لكن + +86 +00:06:42,520 --> 00:06:47,630 +بيشيله بقطرةاللي هو ال flash memory اللي عليها + +87 +00:06:47,630 --> 00:06:52,470 +information software تمام؟ نفس الجثة لما نشوف كمان + +88 +00:06:52,470 --> 00:06:55,430 +شوية ال plasmids هنعرف كيف البكتيريا بتنقل + +89 +00:06:55,430 --> 00:06:58,490 +المعلومات من بكتيريا إلى أخرى عبر ال information + +90 +00:06:58,490 --> 00:07:01,930 +تمام؟ يعني مافيش داعي تعمل محاضرات للآخرين و + +91 +00:07:01,930 --> 00:07:06,530 +تحملهم جميلة لأ بتعطيهم قطعة من المادة الوراثية و + +92 +00:07:06,530 --> 00:07:12,070 +انتهى الموضوعإذن they translate mRNA and produces + +93 +00:07:12,070 --> 00:07:16,590 +the information على شكل البروتين البروتين هو ال + +94 +00:07:16,590 --> 00:07:20,390 +expression للمعلومات الموجودة في ال RNA لأصل + +95 +00:07:20,390 --> 00:07:26,270 +المعلومة المأخوذة أساسا من ال DNA لبلازميت أنا + +96 +00:07:26,270 --> 00:07:32,050 +حاولت التعريف أقطع قطعة صغيرة عشان انزهل المعلومات + +97 +00:07:32,050 --> 00:07:35,330 +هو البلازميت عبارة عن small circular extra + +98 +00:07:35,330 --> 00:07:41,750 +chromosomalخارج نطاق الكروموزوم double-stranded + +99 +00:07:41,750 --> 00:07:45,970 +DNA molecule في حد عنده شك أن ال DNA أصلا double + +100 +00:07:45,970 --> 00:07:51,270 +-stranded ما هو double-stranded و لأ حسيته شك مين + +101 +00:07:51,270 --> 00:07:55,490 +درس فيروسات ولا لسه محدش درس فيروسات في الفيروسات + +102 +00:07:55,490 --> 00:07:59,030 +هناك بعض الفيروسات المادة الوراثية بتكون single + +103 +00:07:59,030 --> 00:08:03,110 +-stranded DNA وبالتالي عملنا stressing يعني تأكيد + +104 +00:08:03,110 --> 00:08:06,910 +على كلمة double-stranded DNA moleculethey are + +105 +00:08:06,910 --> 00:08:11,650 +capable of self-replicate تنقسم ال .. ال .. ال .. + +106 +00:08:11,650 --> 00:08:15,550 +ال plasma independently of the chromosome يعني ال + +107 +00:08:15,550 --> 00:08:19,450 +chromosome بنقسم لحال و هي تنقسم لحال مافيش ارتباط + +108 +00:08:19,450 --> 00:08:24,110 +بينهم مافيش ارتباط بينهم هل من المعقول انه انا + +109 +00:08:24,110 --> 00:08:27,070 +المعلومات اللي عندي في الدماغ اذا بده انقلكوا + +110 +00:08:27,070 --> 00:08:31,360 +إياها اعطيكوا إياها و ابقى من غير المعلومات هذهولا + +111 +00:08:31,360 --> 00:08:35,240 +لازم اعمل نسخ عنها بعمل نسخ و بعطيكوا بحجم النسخ + +112 +00:08:35,240 --> 00:08:37,420 +اللي .. عشان انا كنت بقول انه ال .. ال .. ال + +113 +00:08:37,420 --> 00:08:41,080 +plasma هاي مثلا ال plasma عاملة replication في + +114 +00:08:41,080 --> 00:08:45,020 +اكتر من نسخة في الخلية هي واحدة اتنين تلاتة اربعة + +115 +00:08:45,020 --> 00:08:50,720 +وهذا تمثيل قسمي لمين؟ للكرموزوم يعني ان التوضيح + +116 +00:08:50,720 --> 00:08:54,960 +للكرموزوم اكبر بكتير من مين من ال plasma كمان برضه + +117 +00:08:54,960 --> 00:08:59,540 +small circular extra chromosomal double stranded + +118 +00:08:59,950 --> 00:09:07,250 +DNA capable of self-replication and contains genes + +119 +00:09:07,250 --> 00:09:14,310 +تحتوي على جينات that confer تمنح تعطي some + +120 +00:09:14,310 --> 00:09:18,890 +properties such as antibiotic resistance تخيلوا أن + +121 +00:09:18,890 --> 00:09:23,190 +البكتيريا تتبادل المعرفة في كيفية حماية نفسها من + +122 +00:09:23,190 --> 00:09:28,850 +ال antibiotic بكتيريا بطريقة ما صارت عندها المعرفة + +123 +00:09:30,060 --> 00:09:33,080 +بمقاومة ال .. ال .. ال antibiotic اسمه Benzalic + +124 +00:09:33,080 --> 00:09:37,840 +كيف؟ عن طريق إنتاج أنزين اسمه Penicillinase ال + +125 +00:09:37,840 --> 00:09:41,600 +Penicillinase بيكسر ال Penicillin فبالتالي بيصير + +126 +00:09:41,600 --> 00:09:44,100 +ال Benzaline غير فعال ضد البكتيريا وبالتالي + +127 +00:09:44,100 --> 00:09:47,260 +البكتيريا الآن protected من ال Penicillin صح ولا + +128 +00:09:47,260 --> 00:09:51,200 +مش صح؟ طيب هذه المعلومات وين مخزنة إنتاج ال + +129 +00:09:51,200 --> 00:09:54,980 +Penicillinase على ال plasma فلو نقلت البكتيريا + +130 +00:09:54,980 --> 00:09:59,870 +المقاومةالبلاسمة تبقى حال البكتيريا غير مقاومة + +131 +00:09:59,870 --> 00:10:04,990 +تصبح البكتيريا الأخرى مقاومة زي ما قلت لكوا ال + +132 +00:10:04,990 --> 00:10:10,190 +flash حطيناها في جهاز تمام أخذ من ال flash معلومة + +133 +00:10:10,190 --> 00:10:13,690 +أخذ من الجهاز معلومة كيفية مكافحة فيروس معين فيروس + +134 +00:10:13,690 --> 00:10:19,770 +حسوبي أخدناه وحطيناه في الجهاز التاني الجهاز تنكسب + +135 +00:10:19,770 --> 00:10:24,010 +إخلاء صفة أنه مقاوم لهذا الفيروسطبعا مش بس + +136 +00:10:24,010 --> 00:10:28,550 +antibiotic resistance كمان ممكن تنقل جينات دات + +137 +00:10:28,550 --> 00:10:32,190 +معلومات خاصة بإنتاج سموم على سبيل المثال يعني + +138 +00:10:32,190 --> 00:10:37,490 +بكتيريا بريقة مايلها المقدرة على إنتاج سم لما + +139 +00:10:37,490 --> 00:10:42,150 +نقلناها لبلازميل صلاة تنتج هذا السم أي مادة من + +140 +00:10:42,150 --> 00:10:48,130 +شأنها أن تجعل البكتيريا أكثر شراسة أكثر إمراضة + +141 +00:10:48,130 --> 00:10:54,210 +بيسميها virulence factor أي عامليجعل من البكتيريا + +142 +00:10:54,210 --> 00:10:59,130 +قادرة على إحداث المرض بنسميه Virulence Factor مهم + +143 +00:10:59,130 --> 00:11:03,130 +كتير المعلومة هذه لأنها حد رح نكررها مرارا وتكرار + +144 +00:11:03,130 --> 00:11:07,950 +Plasmids are not essential for the survival of the + +145 +00:11:07,950 --> 00:11:12,990 +cell بمعنى أنه مش ليس ال wall ولا ال cell membrane + +146 +00:11:12,990 --> 00:11:16,330 +ولا ال ribosomes لو مافيش Plasmids ممكن تعيش + +147 +00:11:16,330 --> 00:11:20,150 +البكتيريا بشرط أنه تعيش في ظروف ملائمة + +148 +00:11:23,860 --> 00:11:27,500 +طب هتنتقل البلازميت من خلية لخلية؟ نعم هتنتقل، + +149 +00:11:27,500 --> 00:11:31,100 +هتتكلم عن حسمة conjugation اللي هي الاقتران + +150 +00:11:31,100 --> 00:11:36,640 +الجنسي، خلية مع خلية بتبادلوا المعلومات أنا تحدثت + +151 +00:11:36,640 --> 00:11:39,920 +إن في السترات البلازم، فينا inclusion bodies، وكنا + +152 +00:11:39,920 --> 00:11:44,240 +ال inclusion bodies هي عبارة عن storage granules، + +153 +00:11:44,240 --> 00:11:48,000 +والغرض من ال storage أو الداعي لل storage هو ال + +154 +00:11:48,000 --> 00:11:52,300 +abundance، اللي هو فيه كمية كبيرة موجودة من الغذاء + +155 +00:11:52,300 --> 00:11:58,870 +في المحيططبعا تخزن هذا الغذاء الزائد لحين الحاجة + +156 +00:11:58,870 --> 00:12:01,930 +تمام؟ فيه granules بيسموها storage granules أو بعض + +157 +00:12:01,930 --> 00:12:05,970 +الأحيان inclusions طبعا الحديث عن هذا الموضوع + +158 +00:12:05,970 --> 00:12:08,670 +بيختلف لما اتكلم في ال medical microbial بيختلف عن + +159 +00:12:08,670 --> 00:12:10,990 +.. لما اتكلم عن ال .. ال .. ال environmental فى + +160 +00:12:10,990 --> 00:12:15,730 +بكتيريا عصيمتال بتخزن المغناطسات بيسموها + +161 +00:12:15,730 --> 00:12:20,390 +magnetosomes بتخزن فى حالها فبصير بكتيريا مغناطسية + +162 +00:12:20,390 --> 00:12:24,430 +بيسموها magnetotactic bacteriaالمجنيدوتاكتك + +163 +00:12:24,430 --> 00:12:28,850 +بكتيريا تستخدم الميغناطيسات هذه في الحفاظ على + +164 +00:12:28,850 --> 00:12:33,210 +نفسها في مستوى معين في المياه مثلا في مياه البراكا + +165 +00:12:33,210 --> 00:12:36,970 +أو المياه العادمة تمام؟ بدل ما تطفو على السطح أو + +166 +00:12:36,970 --> 00:12:40,730 +تستقر في القاع بترتب الميغناطيسات بحاجة إنها تكون + +167 +00:12:40,730 --> 00:12:44,130 +في مستوى معين هذا المستوى المعين ملائم إلا من + +168 +00:12:44,130 --> 00:12:50,310 +ناحية الأكسجين يعني وكأنه وكأنه وسيلة anchoring + +169 +00:12:50,310 --> 00:12:55,620 +مرسابترسي نفسها في مكان معين مفضل لها الظروف فهناك + +170 +00:12:55,620 --> 00:13:00,600 +الكثير من ال inclusion granules المتنوعة لكن + +171 +00:13:00,600 --> 00:13:04,780 +معظمها الغرض الأساسي منها إيه يا بنات ال storage + +172 +00:13:04,780 --> 00:13:10,900 +وال storage تبقى إيه؟ تبقى energy molecules هذه + +173 +00:13:10,900 --> 00:13:13,920 +بعض الصور ال electron microscope بتوضح لنا ما فينا + +174 +00:13:13,920 --> 00:13:17,640 +اللي هو inclusion bodies في كثير من أنواع من + +175 +00:13:17,640 --> 00:13:22,260 +البكتير أو granulesأحنا اتفقنا إن أصلا + +176 +00:13:22,260 --> 00:13:25,380 +البروكاريوتس لا تحتوي على نوكلياس فاحنا حاطينا + +177 +00:13:25,380 --> 00:13:29,000 +عنوان نوكلياس عشان ننفيه فقط لغة نقولكوا إنه + +178 +00:13:29,000 --> 00:13:33,640 +lacking nuclear membrane absence of nuclei اللي هي + +179 +00:13:33,640 --> 00:13:38,320 +بقينا نسميها نواة و نوية هه hence known as .. لذلك + +180 +00:13:38,320 --> 00:13:43,020 +النواة في البروكاريوتس تسمى nucleic material أو + +181 +00:13:43,020 --> 00:13:47,240 +نوكليايد انسوا الدم الأخير هذا هو كان مش موجود + +182 +00:13:50,230 --> 00:13:53,990 +الان الصورة اللى أمامكوا هذه، طلعولي عليها، حد + +183 +00:13:53,990 --> 00:14:01,050 +عنده تخيل إيش هذه الصورة، شايفينها أو مش شايفينها؟ + +184 +00:14:01,050 --> 00:14:04,650 +شجرة؟ + +185 +00:14:04,650 --> 00:14:13,770 +عفا مثلا خالد تفضلي، هذه ال chromosome تعلق شريشة + +186 +00:14:13,770 --> 00:14:20,690 +أكوليةلو فرضناه بيمشي + +187 +00:14:20,690 --> 00:14:24,530 +معانا من أول الجامعة لآخر الجامعة والبكتيريا ليست + +188 +00:14:24,530 --> 00:14:30,350 +طولها واحد مايكروم هذا الحجم و هذا التعقيد كله + +189 +00:14:30,350 --> 00:14:33,650 +موجود في واحد مايكروم + +190 +00:14:37,250 --> 00:14:40,410 +اللي أنا قلتلكوا هو single و circular و لما + +191 +00:14:40,410 --> 00:14:44,390 +البكتيريا بده تنتج .. بده تنتج انزين معين ابتعد + +192 +00:14:44,390 --> 00:14:47,590 +تأمر للجين الوحيد اللي موجود لدى الانزين من كل + +193 +00:14:47,590 --> 00:14:52,490 +هالخربة طيارة انه يشتغل small but terrible small + +194 +00:14:52,490 --> 00:14:58,210 +but efficient يعني صغيرة لكن رهيبة صغيرة لكن فعالة + +195 +00:14:58,210 --> 00:15:03,390 +إياكم أن تنظروا للبكتيريا على إنها يعني مخلوق يعني + +196 +00:15:03,390 --> 00:15:09,730 +في السلسلة و انخفضالبكتيريا عندها .. قلنا قبل شوية + +197 +00:15:09,730 --> 00:15:12,970 +في ال energy أشطر من إطلاع الصحراء وبتعرف تنجي + +198 +00:15:12,970 --> 00:15:16,830 +أكلها أكتر من ما نختاره، بتعرف كيف تهرب من المواد + +199 +00:15:16,830 --> 00:15:21,450 +السامة، بتعرف كيف تتكثر بشكل فعال، كيف تخزن، يعني + +200 +00:15:21,450 --> 00:15:25,230 +كل المهارات اللي بيمتلكها البشر، تمام؟ ومن ضمنها + +201 +00:15:25,230 --> 00:15:31,890 +التعليم، تتعلم البكتيريا بشكل جيد من بعضها البعض، + +202 +00:15:31,890 --> 00:15:36,830 +single ال chromosome, circular and compactedعشان + +203 +00:15:36,830 --> 00:15:39,530 +هيكن هذا اللي طوله من الأول جامعة لأخر جامعة + +204 +00:15:39,530 --> 00:15:46,510 +بينضبط كله في واحد مايكن نيجي لتركيب مهم جدا اسمه + +205 +00:15:46,510 --> 00:15:49,330 +ال capsule أو الإصلاح الغلاف و أنا بتمنى عليكم + +206 +00:15:49,330 --> 00:15:53,450 +انكوا يعني اللوحة شوية تبقى قدوة عنها و تنتبهوا + +207 +00:15:53,450 --> 00:15:59,990 +لكلام اللي بتحكي الغلاف + +208 +00:15:59,990 --> 00:16:04,290 +الخارجي اللي أول غلاف بحيط بال cell wall لما طلعنا + +209 +00:16:04,290 --> 00:16:12,400 +برا هو ال capsuleهذه الـ bacteria هذا + +210 +00:16:12,400 --> 00:16:15,580 +الـ structure اللي ورا الـ cell wall بنسميها الـ + +211 +00:16:15,580 --> 00:16:20,180 +capsule العلماء احترفوا تسميته الصحيح يعني لو + +212 +00:16:20,180 --> 00:16:22,980 +طلعتوا على الكتب بتلاقيه capsule، glycocalyx، + +213 +00:16:22,980 --> 00:16:26,660 +slime layer، S layer، micro capsule طب الإختلافات + +214 +00:16:26,660 --> 00:16:30,080 +هذه إيش كانت سببها؟ الإختلافات هذه كانت سببها + +215 +00:16:30,080 --> 00:16:34,680 +شغلتين رئيسيتين ال thickness سماكة + +216 +00:16:36,130 --> 00:16:45,270 +الطبقة وانتظامها السماكة ماسميها thickness و ال + +217 +00:16:45,270 --> 00:16:49,170 +organization اللي + +218 +00:16:49,170 --> 00:16:55,150 +هو تنظيم الكتشوف لحظه هذا regular منتظم لو تخيلنا + +219 +00:16:55,150 --> 00:17:01,850 +تركيب آخر عامل زي هى بنفس ال thickness هذا + +220 +00:17:01,850 --> 00:17:09,210 +منتظم مش منتظمفهل نسمي هذا نفس اسم الكابسول ولا ما + +221 +00:17:09,210 --> 00:17:13,130 +نسموهاش؟ فقال صار في عنا شبه اتفاق، يمكن مش مذكور + +222 +00:17:13,130 --> 00:17:17,750 +قدرنا هنا، حاجة بيسموها glycocalyx أي تركيب، أي + +223 +00:17:17,750 --> 00:17:22,910 +تركيب بينطبق على صفات الكابسول هذه اللي هنا بيسموه + +224 +00:17:22,910 --> 00:17:25,530 +glycocalyx، لكن بيسموه glycocalyx إلى أربعة أصلا، + +225 +00:17:25,530 --> 00:17:29,890 +خلينا نكتبهم على الصرف، glyco + +226 +00:17:35,680 --> 00:17:40,820 +الجليجوكاليكس قد يكون واحد + +227 +00:17:40,820 --> 00:17:45,620 +من أربعة capsule micro + +228 +00:17:45,620 --> 00:17:51,420 +capsule slime + +229 +00:17:51,420 --> 00:17:55,460 +layer و + +230 +00:17:55,460 --> 00:17:58,960 +slime على لزج يعني طبقة لزجة و هي كله أصلا لزجة + +231 +00:17:58,960 --> 00:18:03,000 +هنشوف كمان شوية الوضعات تبعتها و S layer + +232 +00:18:06,040 --> 00:18:13,140 +الان مال اتشابه ومال اختلاف بين الأربع هدول واضح + +233 +00:18:13,140 --> 00:18:16,240 +كابسول وكابسول في بينهم علاقة و layer و layer برضه + +234 +00:18:16,240 --> 00:18:19,480 +فيهم علاقة لعلاقة اللي بينهم هدول لإنه تنتين + +235 +00:18:19,480 --> 00:18:24,320 +organized بينما + +236 +00:18:24,320 --> 00:18:26,680 +ال slime layer لعلاقة اللي بينهم انه none + +237 +00:18:26,680 --> 00:18:34,080 +organized فبدا هذا الشيء ال common ايش الاختلاف؟ + +238 +00:18:35,450 --> 00:18:44,370 +الـ Thickness يعني + +239 +00:18:44,370 --> 00:18:48,750 +الكابسول organized والمايكرو كابسول organized واضح + +240 +00:18:48,750 --> 00:18:52,330 +أن كلمة كابسول هي الأضخم فال thickness يبقى عالي + +241 +00:18:52,330 --> 00:18:55,390 +المايكرو كابسول organized بس ايه ال thickness صغير + +242 +00:18:55,390 --> 00:19:00,670 +ال slime layer مش organized لكن thick ال S layer + +243 +00:19:00,670 --> 00:19:05,060 +مش organized لكن thinو هي الفروقات بين الأربعة، + +244 +00:19:05,060 --> 00:19:10,440 +إذا لو سمعتوا في يوم من الأيام مصطلح glycocalyx، + +245 +00:19:10,440 --> 00:19:14,020 +capsule و لا micro capsule و لا S layer و لا slam + +246 +00:19:14,020 --> 00:19:18,360 +layer، كلهم في النهاية هم اللي أنتوا بتعرفوهم باسم + +247 +00:19:18,360 --> 00:19:22,800 +مين؟ باسم capsule، باسم capsule، لكن معنين إنه + +248 +00:19:22,800 --> 00:19:26,700 +نقلنه، كتير من الكتب صارت تتكلم عنهم بضرر مختلفة و + +249 +00:19:26,700 --> 00:19:29,920 +متنوعة، فكويس إن أنتوا عارفين هذه المعلومة + +250 +00:19:32,340 --> 00:19:36,920 +ما هي .. اه مما اتكون الكابسول هذه نقطة كتير مهمة + +251 +00:19:36,920 --> 00:19:47,240 +ايش التركيب الكيميائي للكابسول مكتوب من اللوحة من + +252 +00:19:47,240 --> 00:19:52,220 +polysaccharide لكن هناك exception هناك exception + +253 +00:19:52,220 --> 00:19:58,600 +هي ال polysaccharide رأيتها؟ when however certain + +254 +00:19:58,600 --> 00:20:03,080 +bacilli they are composed of polypeptideوأجيبلكم + +255 +00:20:03,080 --> 00:20:07,960 +مثال صارخ على هذه البكتيريا عشان تحفظوا اسمها، كم + +256 +00:20:07,960 --> 00:20:12,000 +اسم البكتيريا اللي تحفظينها اليوم؟ ولا واحدة؟ + +257 +00:20:22,290 --> 00:20:27,490 +Bacillus anthracis وحي المسبب لجامرة الخبيثة جامرة + +258 +00:20:27,490 --> 00:20:32,390 +الخبيثة مرض خطير جدا أمريكا أتهمت العراق في حرب + +259 +00:20:32,390 --> 00:20:36,930 +الخليج الأول والثانية على أنه تمتلك مئات الأطنان + +260 +00:20:36,930 --> 00:20:38,790 +من هذه البكتيريا وأنه هتستخدمها في السلاح + +261 +00:20:38,790 --> 00:20:44,450 +البيولوجي فبكتيريا خطيرة الكابسول تبعتها تتكون من + +262 +00:20:44,450 --> 00:20:48,870 +polypeptide خلافا لمعظم أنواع البكتيريا المتكونة + +263 +00:20:48,870 --> 00:20:55,750 +من مين قلنا من polyهتذكركم الأربعة شريشيا كولاي، + +264 +00:20:55,750 --> 00:20:59,270 +Staphylococcus aureus مايكوبلازما اللي ملاقش cell + +265 +00:20:59,270 --> 00:21:03,010 +wall تمام من بارح وهي البكتيريا اللي احنا بتكلم + +266 +00:21:03,010 --> 00:21:11,590 +عنها البسيلاس أنتراسيس أنصحكم بالمتابعة أولا بأول + +267 +00:21:11,590 --> 00:21:17,490 +حتى لا تتركن المعلومات اليوم بعض + +268 +00:21:17,490 --> 00:21:22,950 +الصور لل capsule تبوا معاياهذه صورة electron + +269 +00:21:22,950 --> 00:21:27,350 +microscope تظهر انها كابسول الكابسولة انا اظهرت + +270 +00:21:27,350 --> 00:21:32,510 +انها irregular صح هذه irregular مرة هنا سميكة و + +271 +00:21:32,510 --> 00:21:38,190 +مرة هنا رقيقة فهذه نسميها اما slim layer او s + +272 +00:21:38,190 --> 00:21:42,990 +layer حسب المقارنة في ال thickness بينما هذه رحظة + +273 +00:21:42,990 --> 00:21:50,270 +regular لكن thin فبسموها micro caps الصورتين هدولة + +274 +00:21:50,560 --> 00:21:53,640 +هم عبارة عن صورتين هتشوفوهم في المعمل ان شاء الله + +275 +00:21:53,640 --> 00:21:56,760 +لما تشتغلوا ال capsule stain يعني في احنا في + +276 +00:21:56,760 --> 00:22:02,100 +المعمل جزئية متعلقة بسباقة الكابسول فهنشوف + +277 +00:22:02,100 --> 00:22:07,200 +البكتيريا عبارة عن حاجة hallow او dark و حواليها + +278 +00:22:07,200 --> 00:22:11,540 +منطقة hallow يعني نفس البكتيريا هتكون dark لأنه + +279 +00:22:11,540 --> 00:22:14,820 +بنستخدم ما يسمى بال negative stain ال negative + +280 +00:22:14,820 --> 00:22:20,140 +stain يعنيبنصبغ الخلفية وبنصبغش الخلية نفسها + +281 +00:22:20,140 --> 00:22:23,320 +الخلفية تبعت البكتيريا بتصبغ بينما البكتيريا + +282 +00:22:23,320 --> 00:22:28,500 +لاتصبغ لأن حضرة ال capsule على الرغم من انها + +283 +00:22:28,500 --> 00:22:36,340 +virulence factor مهم جدا اللي انها delicateرقيقة، + +284 +00:22:36,340 --> 00:22:40,940 +هشة، سهل لو عملناها بالمعالجات فكل gram is 10؟ + +285 +00:22:40,940 --> 00:22:44,620 +crystal violet و iodine و Ethanol و بعدين سفرانين، + +286 +00:22:44,620 --> 00:22:48,840 +هذه معالجات صعبة على البكتيريا، لو الكابسول موجودة + +287 +00:22:48,840 --> 00:22:52,880 +ستفقد الكابسول، فنعالجها بطريقة معينة هنشوفها في + +288 +00:22:52,880 --> 00:22:58,880 +المعبنة الشعرية الآن نيجي لل functions of the + +289 +00:22:58,880 --> 00:23:02,920 +capsule، و ركزوا كويس، لإن ال functions هذه كتير + +290 +00:23:02,920 --> 00:23:09,730 +مهمة، رقم واحد، attachmentهنشوف function عكسها + +291 +00:23:09,730 --> 00:23:14,190 +تماما للـ capsule عكس الـ attachment هروب من الـ + +292 +00:23:14,190 --> 00:23:19,170 +attachment مظبوط ان الـ attachment التصاق بنا + +293 +00:23:19,170 --> 00:23:23,490 +تخيلوا معي التخيل هذا الـ urinary tract بتبتدأ من + +294 +00:23:23,490 --> 00:23:27,550 +وين؟ من ال kidneys مظبوط و بتروح الحالب و الحالب + +295 +00:23:27,550 --> 00:23:30,150 +بيصف فيه اللي هو ال .. ال .. ال .. ال urinary + +296 +00:23:30,150 --> 00:23:33,410 +bladder و بعدين ال .. ال .. ال .. الخارج لو + +297 +00:23:33,410 --> 00:23:38,080 +بكتيريا جاءت تتسلق في الـ ureterقطرة بول اعتقد + +298 +00:23:38,080 --> 00:23:41,860 +قطرة البول عارفين حاجة من قطرة طبعا قطرة البول + +299 +00:23:41,860 --> 00:23:45,740 +جداش حجب من المقارنة مع البكتيريا كبير القطرة اللي + +300 +00:23:45,740 --> 00:23:50,900 +ضخمة صح البكتيريا صغيرة خالص فلو نزلت عليها قطرة + +301 +00:23:50,900 --> 00:23:55,940 +تشبيه زي ما واحد جاعت تحت شلالة نياجرة طبعا واحد + +302 +00:23:55,940 --> 00:24:01,060 +واحد منها بيصير بترد الحشرة فبالتالي عملية سقوط + +303 +00:24:01,060 --> 00:24:06,850 +البول من أعلى بتطرد البكتيريا إلى أسفلإلا إذا كان + +304 +00:24:06,850 --> 00:24:12,290 +البكتيريا عندها المقض على إيش؟ على التاجب يبقى لن + +305 +00:24:12,290 --> 00:24:16,750 +يحدث urinary tract infection التهابات مسالك بولية + +306 +00:24:16,750 --> 00:24:21,490 +إلا إلا دي أنواع من البكتيريا تمتلك المقض على مين؟ + +307 +00:24:21,490 --> 00:24:26,170 +على التاجب يبقى ال virulence factor essential + +308 +00:24:26,170 --> 00:24:30,950 +أساسي مهم جدا جدا ان نقول البكتيريا عندها المقض + +309 +00:24:30,950 --> 00:24:33,270 +على الالتصاف طيب + +310 +00:24:36,380 --> 00:24:39,380 +لما ندرس diagnostic microbiology أو حتى في ال .. + +311 +00:24:39,380 --> 00:24:42,500 +في ال medical microbiology ان شاء الله، هنتعرف على + +312 +00:24:42,500 --> 00:24:46,760 +بعض المصطلحات منها infection، مصطلح infection، + +313 +00:24:46,760 --> 00:24:49,600 +هتلاقوا ان ال infection عبارة عن successful + +314 +00:24:49,600 --> 00:24:54,180 +colonization، طب ايش colonization؟ successful + +315 +00:24:54,180 --> 00:24:59,000 +existence في مكان لم تكن مولد فيه بكتير سابقاالـ + +316 +00:24:59,000 --> 00:25:01,800 +Successful هذا مع الـ Successful هذا بيقدّوا إلى + +317 +00:25:01,800 --> 00:25:05,720 +أو قد يقدّوا إلى disease لكن لن يكون هناك success + +318 +00:25:05,720 --> 00:25:09,860 +إلا ده كان فيه إمكانية attachment البكتيريا عشان + +319 +00:25:09,860 --> 00:25:14,640 +تتغذى لازم تلتصق إلا إذا كان في بيئة سائل، بيئة + +320 +00:25:14,640 --> 00:25:19,640 +جسد بتلتصق على خلايا عشان تتغذى على هذه الخلايا + +321 +00:25:19,640 --> 00:25:25,110 +لأن التصاق عملية مهمةthese structures are thought + +322 +00:25:25,110 --> 00:25:28,110 +to be to help cell attached to their target + +323 +00:25:28,110 --> 00:25:31,870 +environment target environment يعني و هذا و هذا + +324 +00:25:31,870 --> 00:25:34,210 +اخدوا بأكوا ال attachment بيكون عبارة عن receptor + +325 +00:25:34,210 --> 00:25:38,430 +receptor يعني مش التصاق لإنه ال .. لإنه ال .. ال + +326 +00:25:38,430 --> 00:25:43,490 +.. ال capsule لذجة لأ الاتصاق بيكون عبر ايه؟ + +327 +00:25:43,490 --> 00:25:51,090 +receptor يعني فيها receptor للأنزل مثال عليهابصحى + +328 +00:25:51,090 --> 00:25:56,990 +اسم جديد mutants produces a slime layer in the + +329 +00:25:56,990 --> 00:26:02,510 +presence of sucrose اللي بتشوفوا أصغار أطفال ماراش + +330 +00:26:02,510 --> 00:26:06,410 +على المدرسة و أسنانه كلها مسوسة مصبوط تسأل أمه + +331 +00:26:06,410 --> 00:26:10,390 +بيقولك هذه ما هو بيأكلش غير حلو الحلو رايش انه حلو + +332 +00:26:10,390 --> 00:26:16,890 +سكر و السكر اللي بنعرفه سكروز فالسكروز اللي هو + +333 +00:26:16,890 --> 00:26:20,460 +السكرلو البكتيريا اللي اسمها streptococcus mutants + +334 +00:26:20,460 --> 00:26:25,820 +وهي تعيش بالفن بالمناسبة حضرتها في وجود ال .. في + +335 +00:26:25,820 --> 00:26:28,480 +وجود ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +336 +00:26:28,480 --> 00:26:28,500 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +337 +00:26:28,500 --> 00:26:28,900 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +338 +00:26:28,900 --> 00:26:29,320 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +339 +00:26:29,320 --> 00:26:29,860 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +340 +00:26:29,860 --> 00:26:30,000 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +341 +00:26:30,000 --> 00:26:30,400 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +342 +00:26:30,400 --> 00:26:30,620 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +343 +00:26:30,620 --> 00:26:31,020 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +344 +00:26:31,020 --> 00:26:32,880 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +345 +00:26:32,880 --> 00:26:38,720 +ال .. ال .. ال .. ال .. ال .. + +346 +00:26:38,720 --> 00:26:39,720 +ال .. + +347 +00:26:48,350 --> 00:26:52,010 +and many bacteria can stick to tooth surfaces and + +348 +00:26:52,010 --> 00:26:52,830 +cause decay + +349 +00:27:00,170 --> 00:27:03,990 +لما بتتكاثر البكتيريا و بتتغذى على ال carbohydrate + +350 +00:27:03,990 --> 00:27:09,830 +بتنتج acid، ال acid بيتفاعل مع ال calcium اللي + +351 +00:27:09,830 --> 00:27:13,930 +موجود في الأسنان، فبياخد ال calcium من الأسنان، + +352 +00:27:13,930 --> 00:27:16,110 +لما ياخد ال calcium من الأسنان هو مين اللي بيخلي + +353 +00:27:16,110 --> 00:27:20,310 +الأسنان قوية و صلبة؟ ال calcium، وبالتالي تبدأ هنا + +354 +00:27:20,310 --> 00:27:24,990 +التشوهات أو التسوّس اللي احنا بيسميه تسوّس الأسنان + +355 +00:27:24,990 --> 00:27:28,790 +أو ما يسمى ب dental caries، dental caries، تسوّس + +356 +00:27:28,790 --> 00:27:34,830 +الأسنانخدنا مثال على مين؟ على الـ streptococcus + +357 +00:27:34,830 --> 00:27:39,050 +mutants في مثال تاني على حسب الـ Vibrio cholera و + +358 +00:27:39,050 --> 00:27:42,070 +ال Vibrio cholera من اسمها بتسبب مرض الكوليرة + +359 +00:27:42,070 --> 00:27:47,250 +إسهال شديد جدا يقتل الأطفال في كثير من البلدان في + +360 +00:27:47,250 --> 00:27:50,950 +العالم و عندنا في فلسطين حدثت مجموعة من الأوبياء + +361 +00:27:50,950 --> 00:27:54,890 +إلى الكوليرة لكن ربنا سلم يعني ما كان فيه نسبة + +362 +00:27:54,890 --> 00:28:00,870 +انتشار واسع also produces a glycocalyxwhich helps + +363 +00:28:00,870 --> 00:28:04,830 +to attach to the intestinal villi in the host يعني + +364 +00:28:04,830 --> 00:28:09,690 +بأهلها ترتبط في ال intestinal villi داخل العقل + +365 +00:28:09,690 --> 00:28:15,310 +وإلا وإلا مع الإسهال كل ال vibro هتطلع برا، صح + +366 +00:28:15,310 --> 00:28:19,590 +ولا؟ وبتلبط لل disease، وخد بالك الإسهال أصلا هو + +367 +00:28:19,590 --> 00:28:23,970 +ميكانيزم من الجسم دفاعية للطرد، لكن في كثير من + +368 +00:28:23,970 --> 00:28:27,010 +الأحيان بتزيد الردد الفعل من عينا لدرجة أنها + +369 +00:28:27,010 --> 00:28:31,190 +تستهلك، تستهلكال .. ال .. ال electrolytes و الماء + +370 +00:28:31,190 --> 00:28:35,630 +فبموت .. بموت الشخص مش من البكتيرا و الصوم تبعها و + +371 +00:28:35,630 --> 00:28:38,590 +أنها مرض الفعل تبعاتنا الطبيعية ما نسميه الجفاف و + +372 +00:28:38,590 --> 00:28:41,110 +.. و .. و اختلال ال electrolyte اللي بيسموه + +373 +00:28:41,110 --> 00:28:45,730 +electrolyte imbalance يبقى اتفاقنا على function + +374 +00:28:45,730 --> 00:28:49,770 +أخر واحد اسمها attachment نيجي بالتانية protection + +375 +00:28:49,770 --> 00:28:53,890 +from phagocytic engulfing كل كوب ماد غسلته بصابون + +376 +00:28:53,890 --> 00:28:58,630 +ايداكه بصابون مش سائل صابون قطعوكلكوا أكيد في + +377 +00:28:58,630 --> 00:29:02,310 +حياتكوا مرة عليكم بتكونوا أول من يفتح الصابون + +378 +00:29:02,310 --> 00:29:05,330 +بينكوا بتشيل ورد عنها صح ولا لأ؟ إذا بتشيل ورد + +379 +00:29:05,330 --> 00:29:10,390 +بتمسكيها بتجع منك، لكن أولادكوا هتتبلل، وأول + +380 +00:29:10,390 --> 00:29:13,930 +حركتين اللي الصابون نقطة من هنا أو قفزت هنا، + +381 +00:29:13,930 --> 00:29:19,170 +مظبوط؟ ليش؟ لأنها أصبحت لذية تخيلوا البكتيريا، هي + +382 +00:29:19,170 --> 00:29:22,830 +البكتيريا، وعليها الطبقة اللذية، بتجي فجوه ساعت؟ + +383 +00:29:23,010 --> 00:29:26,930 +بتلتقمها، ما بتلتقمها، بتضغط عليها اللي هي هارب + +384 +00:29:26,930 --> 00:29:31,230 +منها، فأهم وضع .. عكس ال attachment صح؟ ال + +385 +00:29:31,230 --> 00:29:33,350 +attachment كانت عبارة عن يجب أنها receptor to + +386 +00:29:33,350 --> 00:29:37,690 +receptor، هنا لإنها لازجة هاربت من الفاجوسيتوس، و + +387 +00:29:37,690 --> 00:29:43,010 +أنا هنا مضطر أجف و أوضح نقطة أساسية في تعامل + +388 +00:29:43,010 --> 00:29:45,590 +الإنسان أو البكتيريا مع الإنسان أو الحيوان أو مع + +389 +00:29:45,590 --> 00:29:46,290 +الhost يعني + +390 +00:29:50,840 --> 00:29:55,920 +أنتم الأن equal من ناحية الشراسة، تمام؟ لاتمتلكون + +391 +00:29:55,920 --> 00:29:58,920 +عوامل شراسة غير أسنانكم، وفي المدارس وانتم صاحبين + +392 +00:29:58,920 --> 00:30:03,700 +الشعب، مظبوط؟ الآن عوامل الشراسة، نفترض أننا + +393 +00:30:03,700 --> 00:30:05,740 +أعطينا زميلتكم مسدس، لأن انتوا عارفين أننا نبيئة + +394 +00:30:05,740 --> 00:30:12,500 +نبيئة المسدس هذا، الآن المسدس هذا أعطاها قدرة على + +395 +00:30:12,500 --> 00:30:15,960 +إحداث ضرر للآخرين، نسمي المسدس عامل شراسة، + +396 +00:30:15,960 --> 00:30:20,520 +أعطاناها سكين،برضه أعطناها عامل قوة، عامل شراسة، + +397 +00:30:20,520 --> 00:30:25,040 +سميناها violence factor، أعطناها واقع لرصاص، يبقى + +398 +00:30:25,040 --> 00:30:29,400 +أعطاناها إيش الآن؟ عامل شراسة للحماية من المسدس، + +399 +00:30:29,400 --> 00:30:31,840 +وبالتالي برضه عامل شراسة، أعطتها قوة، أصلا + +400 +00:30:31,840 --> 00:30:35,860 +اتهاجموا يا مش خايفة، أمان؟ اللي أنا دقان .. اللي + +401 +00:30:35,860 --> 00:30:40,080 +أنا وضحته إنه في أسلحة كانت هجومية، مسدس، سكين، + +402 +00:30:40,080 --> 00:30:44,150 +وكان فيه إيش الآن أنا؟ أسلحة دفاعيةالحرب بيننا + +403 +00:30:44,150 --> 00:30:49,930 +وبين البكتيريا بهذه البساطة هي تمتلك أسلحة هجومية + +404 +00:30:49,930 --> 00:30:54,410 +وأسلحة دفاعية ونحن كذلك نمتلك أسلحة هجومية والدليل + +405 +00:30:54,410 --> 00:30:58,430 +على ذلك أننا الفاجو والسايتس والماكروفاج ابتلتقم + +406 +00:30:58,430 --> 00:31:02,870 +وابتقتل صح؟ هي عندها الكابسول اللي تهرب عندها + +407 +00:31:02,870 --> 00:31:06,410 +الكابسول اللي تهرب عندها التكسين اللي تقتل عندها + +408 +00:31:06,410 --> 00:31:09,790 +أسلحة هجومية تكسين وعندها أسلحة دفاعية مثلا زي + +409 +00:31:09,790 --> 00:31:15,060 +اللي هو الكابسولو سينا في الحرب فيه تمويه و خداع + +410 +00:31:15,060 --> 00:31:19,840 +البكتيريا هناك أنواع منها رائعة جدا في الخداع + +411 +00:31:19,840 --> 00:31:26,540 +disguise يعني ماكرة في الهروب من جهاز المناع زي ما + +412 +00:31:26,540 --> 00:31:32,820 +كان في بعض الاجتياحات سير في دخول من بيت لبيت دون + +413 +00:31:32,820 --> 00:31:35,680 +الخروج للشارع كان يهدموا البيت و يخرجوا للبيت + +414 +00:31:35,680 --> 00:31:39,350 +الآخر لماذا؟ لأن في مقاومين في الشارعفلو خرجنا من + +415 +00:31:39,350 --> 00:31:43,870 +الشارع في عرضة هناك أنواع من البكتيريا تنتقل من + +416 +00:31:43,870 --> 00:31:48,790 +خلية إلى خلية دون الخروج منها تمام؟ بدون تدمير + +417 +00:31:48,790 --> 00:31:54,450 +الخلية الأولى كمان يعني اختراق جزء بسيط و تستطيع + +418 +00:31:54,450 --> 00:31:57,270 +.. هذا هروب من جهاز المنع و ليس هروب .. هروب من + +419 +00:31:57,270 --> 00:32:02,230 +جهاز المنع إذا بدنا نفهم أن عملية ال pathogenesis + +420 +00:32:02,230 --> 00:32:06,990 +في البكتيريا هي عمليةwar مفتوحة حرب مفتوحة بين + +421 +00:32:06,990 --> 00:32:10,750 +الطلافين جهاز المناعة و .. و .. واخدوا بالك و احنا + +422 +00:32:10,750 --> 00:32:16,170 +دايما غالبين، دايما فايزين، متى بنهزم لما نشهر ان + +423 +00:32:16,170 --> 00:32:21,570 +احنا مرضنا؟ بنقول ان هزمنا، اما الان المعرك شغالة + +424 +00:32:21,570 --> 00:32:26,220 +في فمكوا، في أمعاكوا، في الجلد تبعكواشغال المعرك + +425 +00:32:26,220 --> 00:32:30,160 +لأنه أسلحة و عندهم أسلحة و طيبهم بيشتغلوا إحنا ما + +426 +00:32:30,160 --> 00:32:33,560 +بيشغلوش بيد المعرك إلا عندما نمرض و نكون في هذه + +427 +00:32:33,560 --> 00:32:36,900 +الحالة البكتيريا أو الفيروس قد فازة على جهاز + +428 +00:32:36,900 --> 00:32:41,200 +المنحى إذا اتفقنا على أنه فإننا ايش لغاية؟ فإننا + +429 +00:32:41,200 --> 00:32:44,080 +viral infectio اسمه capsule لإنه بيهرب من مين؟ من + +430 +00:32:44,080 --> 00:32:46,700 +الفاجو و سايد أوسسا ال viral infectio هو باب ال + +431 +00:32:46,700 --> 00:32:50,540 +attachment صح؟ بدون attachment صعبة البكتيريا تنجح + +432 +00:32:50,540 --> 00:32:56,050 +تظل في مكانها للطبيعيمن أشهر الميكروبات اللي بتدل + +433 +00:32:56,050 --> 00:32:58,990 +عليها القضية على الأمنية حاجة اسمها Streptococcus + +434 +00:32:58,990 --> 00:33:02,650 +pneumonia ومن اسمها pneumonia تعمل اتهاب نيومونيا + +435 +00:33:02,650 --> 00:33:06,330 +تركزوا معايا في هذه الدزية في صنفين من هذه + +436 +00:33:06,330 --> 00:33:11,110 +البكتيريا صنف له كابسول وصنف مالوش كابسول لما + +437 +00:33:11,110 --> 00:33:17,150 +حقنهم في فارين اللي إلها كابسول قتلت الفار واللي + +438 +00:33:17,150 --> 00:33:21,290 +مالهاش كابسول هو كأنه ماحقناش الفار بحاجة عاش + +439 +00:33:21,290 --> 00:33:25,620 +الفار، تمام؟يبجى إيش كان الفرق بين اتنين؟ ورود + +440 +00:33:25,620 --> 00:33:29,080 +الكابسول يبجى الكابسول كانت مسئولة في هذه + +441 +00:33:29,080 --> 00:33:32,280 +البكتيريا عن إنها تكون pathogenic أو non + +442 +00:33:32,280 --> 00:33:38,820 +pathogenic هذا مثال صارخ على أهمية الكابسول في ال + +443 +00:33:38,820 --> 00:33:43,200 +virus هذا + +444 +00:33:43,200 --> 00:33:46,140 +الجملة نفسه بيننا اللي فوق مافيش فيها أي إذن + +445 +00:33:46,140 --> 00:33:52,560 +function تلاتة resistance to dryيعني فين انت مقاوم + +446 +00:33:52,560 --> 00:33:55,100 +لمين؟ للجفاف ركزوا معايا في هذا الـ Disease إيه + +447 +00:33:55,100 --> 00:33:58,740 +بقى الآن إحنا لما Stripped Coccy pneumonia وان + +448 +00:33:58,740 --> 00:34:03,180 +موجودة في ال lungs بعتنا عامة انها Disease لما + +449 +00:34:03,180 --> 00:34:10,400 +بنكح مش بنطلع مع الكحة البكتيريا بنشوفها في البيئة + +450 +00:34:10,400 --> 00:34:15,080 +لو البكتيريا جفت تمام الجفاف أحد عوام القتل + +451 +00:34:15,080 --> 00:34:18,760 +للبكتيريا بنجففها بنموتها نشيل منها الميه بنموتها + +452 +00:34:20,540 --> 00:34:26,080 +فوجود capsule يجعل من تجفيف البكتيريا أمر ليس + +453 +00:34:26,080 --> 00:34:31,640 +بالسهل وبالتالي يعني بتوفر برضه حماية للبكتيريا في + +454 +00:34:31,640 --> 00:34:36,920 +البيئة كل ما طولت في البيئة كل ما زاد فرصتها + +455 +00:34:36,920 --> 00:34:42,140 +لانتقال لأشخاص آخرين صح ولا لأ؟ يعني نفترض أنه + +456 +00:34:42,140 --> 00:34:47,560 +البكتيريا قدرتها على البقاعة تلات ساعات أنا كحيت + +457 +00:34:47,560 --> 00:34:51,600 +في القاعة تمام؟ بعد ما انت طلعتو القاعة هذه مش + +458 +00:34:51,600 --> 00:34:55,720 +هيزورها حد الا تاني يوم البكتيريا نكحتها هتقعد + +459 +00:34:55,720 --> 00:34:59,980 +تاني يوم تقعد اهل تاني يوم لأنه تلت ساعة فبالتالي + +460 +00:34:59,980 --> 00:35:07,960 +كل ما طالت فترة بقاء البكتيريا حية في البيئة كل ما + +461 +00:35:07,960 --> 00:35:11,360 +زاد إمكانية الجنة انها تنتقل لhost تاني يوم + +462 +00:35:19,990 --> 00:35:24,930 +It is a reservoir مخزن for certain nutrients لبعض + +463 +00:35:24,930 --> 00:35:29,530 +أنواع من المواد المغذية الـ glycocalyx هاله توضيح + +464 +00:35:29,530 --> 00:35:33,070 +لها بونا يكون قادر على أن يرتبط ببعض الأيونات و + +465 +00:35:33,070 --> 00:35:36,870 +المونيكيولز these can then be made available to + +466 +00:35:36,870 --> 00:35:42,190 +the cells هاي الخلية البكتيرية هاي ال slime layer + +467 +00:35:42,190 --> 00:35:48,070 +تمام لأن بتاعنا في بعض الأحيان مينقدرش احنا نتابع + +468 +00:35:48,610 --> 00:35:52,410 +حجم المعلومات اللي جايه هنا ماعطي محاضرة سريعين + +469 +00:35:52,410 --> 00:35:55,490 +جدا اه؟ فاشي بنتجه ايش بنسويها هنا؟ بنسجلها + +470 +00:35:55,490 --> 00:36:01,010 +المحاضرة و بنسمع لها لاحقا لإن انا قاعد بكتب في + +471 +00:36:01,010 --> 00:36:04,970 +المحاضرة قاعد بكتب و بحاول أفهم و بتفرج و كده أكتر + +472 +00:36:04,970 --> 00:36:09,450 +من الشهر اللي بعملها فال capsule ممكن في حال تدفق + +473 +00:36:09,450 --> 00:36:14,950 +كثير من ال molecules حولها انها تلتصق فيها و + +474 +00:36:14,950 --> 00:36:19,940 +تخليها موجودة لحينفضعوط الخلية تستوعبهم إلى الداخل + +475 +00:36:19,940 --> 00:36:25,760 +يعني مخزن خارجي .. مخزن خارجي لبعض الموليكيات طيب + +476 +00:36:25,760 --> 00:36:35,020 +في كمان لسه مخزن او وظيفة خاجسة Depot for waste + +477 +00:36:35,020 --> 00:36:40,280 +product يعني برضه كمان يعني مكاب .. خلينا نقول او + +478 +00:36:40,280 --> 00:36:44,740 +release .. slow release لل waste product waste + +479 +00:36:44,740 --> 00:36:47,280 +products of metabolism are executed from the cell + +480 +00:36:47,550 --> 00:36:51,290 +لأن ال waste product بصير إخراج إليها إلى خارج + +481 +00:36:51,290 --> 00:36:55,430 +الخلية and will accumulate in the capsule حاسف + +482 +00:36:55,430 --> 00:36:59,910 +يهدرعكوا أين؟ في ال capsule this wind them up and + +483 +00:36:59,910 --> 00:37:02,910 +prevent the waste from interfering with cell + +484 +00:37:02,910 --> 00:37:09,030 +metabolism قلنا إنه الإرتباط لل waste products + +485 +00:37:09,030 --> 00:37:15,640 +خارج الخليةبيجعلهم غير قادرين على قضية الخلية ال + +486 +00:37:15,640 --> 00:37:18,840 +waste product خدوا بالك معروف إن ال waste بيقضي + +487 +00:37:18,840 --> 00:37:22,940 +الخلية نفسه فارتباطهم وكأنه بنجمحهم في مكان واحد + +488 +00:37:22,940 --> 00:37:31,340 +بنبعدهم عن مين؟ عن ال metabolism تبع الخلية خمس + +489 +00:37:31,340 --> 00:37:37,380 +وظائف ل ال flagella عفوا، ده الكابسول وظائف قريعة + +490 +00:37:37,380 --> 00:37:42,480 +جدا تجعل من الكابسول بحق أحدال virus factors + +491 +00:37:42,480 --> 00:37:47,620 +المهمة سواء كان في ال infection كattachment أو + +492 +00:37:47,620 --> 00:37:54,020 +كالهروب من ال immune system زي ما قلنا لهروب + +493 +00:37:54,020 --> 00:38:00,320 +من ال phagocytosis أو انها تتسق في المواد وتخزينها + +494 +00:38:00,320 --> 00:38:04,230 +لحين استخدامهاأو لل waste product كمان أيضا عشان + +495 +00:38:04,230 --> 00:38:08,090 +انك تتخلص من السمنية تبعك نجي الان لتركيب اخر برضه + +496 +00:38:08,090 --> 00:38:12,270 +من التراكيب العظيمة فيه البكتيريا واسمه flashlight + +497 +00:38:12,270 --> 00:38:17,410 +بتسمى هو بحاجة اسمه nano technology nanotechnology + +498 +00:38:17,410 --> 00:38:21,790 +تكنولوجيا ال nano اللي هو العربية وهي توظيف جزيئات + +499 +00:38:21,790 --> 00:38:31,220 +بمقياس nano وعمل اجهزة الاجهزة هذه عبارة عنيعني + +500 +00:38:31,220 --> 00:38:37,160 +حجم من واحد nano لميت nano أجهزة تخيلوا موتور كهرة + +501 +00:38:37,160 --> 00:38:44,200 +غير مرئي الفلاجلة الفلاجلة هي عبارة عن nano motor + +502 +00:38:44,200 --> 00:38:47,760 +nano motor يعني موتور nano يعني بينطبق عالي تعريف + +503 +00:38:47,760 --> 00:38:50,320 +ال nano technology بس مش احنا اللي صنعناها مين + +504 +00:38:50,320 --> 00:38:55,970 +اللي صنعه؟ رب العالمموجود في البكتيريا nanomotor + +505 +00:38:55,970 --> 00:38:58,610 +نانو موتور هنشوف كمان شوية بس خلينا نطلع على بعض + +506 +00:38:58,610 --> 00:39:02,350 +نعرفها some bacterial species are mobile and + +507 +00:39:02,350 --> 00:39:06,770 +possesses locomotory organ locomotory يعني حركة + +508 +00:39:06,770 --> 00:39:12,130 +أمام organ يعني organelles اللي هي عضية الحركة + +509 +00:39:12,130 --> 00:39:17,740 +اسمها flagellaكمان مرة some مش كل البكتيريا، يعني + +510 +00:39:17,740 --> 00:39:21,540 +خبنا كده نقول من الآن إن بعض البكتيريا متحرك و + +511 +00:39:21,540 --> 00:39:27,340 +البعض الآخر غير متحرك، لكن معظم البكتيريا المتحركة + +512 +00:39:27,340 --> 00:39:31,780 +متحركة ب flagella، بمعنى آخر أحد أهم أعضاء الحركة + +513 +00:39:31,780 --> 00:39:35,480 +في البكتيريا هو ال flagella، تظهرونك أعضاء أخرى، + +514 +00:39:35,480 --> 00:39:42,380 +نعم، هتورقلها لاحقا إن شاء اللهthose that do not + +515 +00:39:42,380 --> 00:39:46,000 +are able to taste their environment and response + +516 +00:39:46,000 --> 00:39:49,120 +to specific chemical good stuff or toxic material + +517 +00:39:49,120 --> 00:39:54,660 +and move towards or away from them كلمة move + +518 +00:39:54,660 --> 00:40:00,780 +toward or away from a stimuli بنسميها chemotaxis + +519 +00:40:00,780 --> 00:40:05,640 +إيش يعني chemotaxis الإنتذاب إلى أو الإبتعاد عن + +520 +00:40:05,640 --> 00:40:11,700 +stimuliتخيلوا لو واحد مقعد وقالته نار هبط عليه نار + +521 +00:40:11,700 --> 00:40:17,720 +وممكنه يهرب منها بلاش تفطوره موجود غاد في المنطقة + +522 +00:40:17,720 --> 00:40:23,440 +ديك هل بيقدر ينتقل لإذا كان معاه organ of motility + +523 +00:40:23,440 --> 00:40:30,480 +احنا البشر نمشي نتحرك باتجاه أو بالابتعاد بكتير + +524 +00:40:30,480 --> 00:40:34,290 +غير متحركة لاتمتلك هذه الرفاهيةإذا بكنت أعرف من + +525 +00:40:34,290 --> 00:40:38,410 +الآن إن البكتيريا المتحركة فيها صفة إيجابية، ألا + +526 +00:40:38,410 --> 00:40:42,950 +وهي الإبتعاد أو الذهاب إلى، والتنتين فيهم خير + +527 +00:40:42,950 --> 00:40:47,490 +للبكتيريا، صح أو لا، تروح على غذاء أو تبتعد عن خطر + +528 +00:40:47,490 --> 00:40:52,090 +معين الفلاجلة are embedded in the cell membrane، + +529 +00:40:52,090 --> 00:40:55,550 +يبقى منطقة الفلاجلة، من ال cell wall؟ لأ، مورودة + +530 +00:40:55,550 --> 00:40:58,090 +في ال cell membrane، عرفين إيش بال cell membrane؟ + +531 +00:40:58,090 --> 00:41:03,130 +لو تذكرت وظائف ال cell membrane، حتى حد جاوبونيأحد + +532 +00:41:03,130 --> 00:41:07,030 +وظائف ال cell membrane هو energy production و انا + +533 +00:41:07,030 --> 00:41:09,970 +جاي ابقى بقولكوا الفلاجلة عبارة عن موتور الموتور + +534 +00:41:09,970 --> 00:41:13,110 +مش بده طاقة فمنهم ياخد طاقة من ال cell membrane + +535 +00:41:13,110 --> 00:41:16,730 +لذلك هو embedded مغروس في ال cell membrane لكن + +536 +00:41:16,730 --> 00:41:21,150 +الفلاجلة is protruding خارج من إطار اللي هو ال + +537 +00:41:21,150 --> 00:41:23,550 +cell membrane إلى ال cell wall إلى خارج الإطار + +538 +00:41:23,550 --> 00:41:29,620 +هتشوف صورة إن شاء اللهflagella consist of a number + +539 +00:41:29,620 --> 00:41:34,200 +of proteins including flagellin هو ال main protein + +540 +00:41:34,200 --> 00:41:39,020 +الأساسي لل flagella سهل اسمه flagellin طبعا زي ال + +541 +00:41:39,020 --> 00:41:41,400 +protein تبع البلاي اسمه pellin فبالتالي مافيش + +542 +00:41:41,400 --> 00:41:45,560 +صعوبة في ان تحفظ ال flagellin هو ال protein تبع ال + +543 +00:41:45,560 --> 00:41:49,600 +flagella they move the cell by rotating with a + +544 +00:41:49,600 --> 00:41:54,080 +propeller like action وهذه النقطة بدنا نفهمها هي + +545 +00:41:54,080 --> 00:41:58,170 +الخلية وهي ال flagellaالفلاجلة عبارة عن .. انت + +546 +00:41:58,170 --> 00:42:02,770 +عارفين؟ حاجة مرمة، صوت، تمام؟ فالآن تتحرك الفلاجلة + +547 +00:42:02,770 --> 00:42:08,310 +بهذا الشكل وكأنها بتشتغل بطريقة دافعة، طريقة عبارة + +548 +00:42:08,310 --> 00:42:11,450 +عن موتور بتتحرك بهذا الشكل، فبيدفع البكتيريا إلى + +549 +00:42:11,450 --> 00:42:15,270 +الأمام، طبعا برضه ه .. هعرضلكم المحاضرة الجاية ان + +550 +00:42:15,270 --> 00:42:17,190 +شاء الله بعض الفلاشات، انها هتكون المحاضرة الجاية + +551 +00:42:17,190 --> 00:42:22,550 +غالباتوضيحات لل structure تبع ال .. يعني .. يعني + +552 +00:42:22,550 --> 00:42:25,710 +كله متعلق بين المحاضرة السابقة، هعوضكوا بعض + +553 +00:42:25,710 --> 00:42:28,890 +التلاشات ان شاء الله التوضيحية اللي تزيد من فهمكم + +554 +00:42:28,890 --> 00:42:33,830 +لخلية البكتيريا، لأنه احنا معنيين جدا جدا ان + +555 +00:42:33,830 --> 00:42:37,970 +الطالب يخرج من هذا الجزء عارف ايش يعني bacterial + +556 +00:42:37,970 --> 00:42:42,880 +cell structure، مش حافظه بس، فاهم، لأنهكثير من + +557 +00:42:42,880 --> 00:42:47,840 +القضايا المتعلقة في ال medical microbiology مرتبطة + +558 +00:42:47,840 --> 00:42:52,060 +أساسا بفهمنا ل ال bacterial cell structure تشوفوا + +559 +00:42:52,060 --> 00:43:00,560 +الآن السرعات التقريبية لبعض الكائنات، تحفظ تشيتا، + +560 +00:43:00,560 --> 00:43:06,320 +تشيتا هو نوع من فهود، طبعا، وقلنا بسرعتها the + +561 +00:43:06,320 --> 00:43:11,710 +hour، 111 كيلومتر للساعةbody length per second + +562 +00:43:11,710 --> 00:43:17,710 +يعني أكم قفزة في طول جسمها لأ قداش المسافة اللى + +563 +00:43:17,710 --> 00:43:23,230 +بتقطعها طول جسمها طول جسمها 25 مرة في الثانية طول + +564 +00:43:23,230 --> 00:43:28,850 +جسمها المسافة ال human كام مرة 5 و 4 طول جسمه + +565 +00:43:28,850 --> 00:43:32,390 +البكتيريا طالع للسرعة تبعتها point zero zero zero + +566 +00:43:32,390 --> 00:43:37,310 +zero one five kilometer طبعا بالنسبة لحجمها + +567 +00:43:37,310 --> 00:43:41,210 +بالنسبة لحجمهاهذه صورة عمهولة جدا بالنسبة لحجمها + +568 +00:43:41,210 --> 00:43:44,930 +صورة عمهولة جدا لإنه لاحظوا برضه أسرع من البشر + +569 +00:43:44,930 --> 00:43:51,450 +همام أسرع من الإنسان لكن حجم الإنسان بيعطيه قطع + +570 +00:43:51,450 --> 00:43:56,430 +مسافة أكبر لإنه بيبقى .. يعني العملية relative to + +571 +00:43:56,430 --> 00:44:00,690 +the size of the organism ال cheetah خمسة عشرين مرة + +572 +00:44:00,690 --> 00:44:05,730 +أسلب جسمه في الثانية الواحدة الإنسان خمسة وأربع + +573 +00:44:05,730 --> 00:44:07,010 +هذه عشر مرة + +574 +00:44:30,910 --> 00:44:34,390 +هذا الشكل اللي قدامكوا هو الربوط وهنا ال bacterial + +575 +00:44:34,390 --> 00:44:38,070 +cell structure او bacterial structure طالع منه + +576 +00:44:38,070 --> 00:44:41,310 +مجموعة من الفرجلة الفرجلة مش واحدة طلعتها اكتر من + +577 +00:44:41,310 --> 00:44:44,570 +ما احنا مظبوط هنشوف انه انه بالفعل في بعض الخلايا + +578 +00:44:44,570 --> 00:44:47,690 +فيها بيطلع منها فرجلة واحد بيطلع منها عن طرفين + +579 +00:44:47,690 --> 00:44:53,570 +حوالي انبت بيختلف من cell لاخر طلعوا لها الشكل هذا + +580 +00:44:53,570 --> 00:44:57,690 +اللي للأسف مش واضح كتير أمامنا لكن هو عبارة عن + +581 +00:44:57,690 --> 00:45:04,210 +أقراس بتشكل اللي هوال .. ال .. cell membrane + +582 +00:45:04,210 --> 00:45:09,570 +embedded motor يعني الموتور اللي مغروس فيه في ال + +583 +00:45:09,570 --> 00:45:12,770 +cell membrane وانا فيه عندي هذا ال hook اللي هو زي + +584 +00:45:12,770 --> 00:45:16,190 +خطاف وانا فيه عندي اللي هو ال filament يعني أكتر + +585 +00:45:16,190 --> 00:45:18,930 +.. أكتر .. أكتر من قسم بين .. بين قسم بين ال ثلاث + +586 +00:45:18,930 --> 00:45:29,710 +أقسام نرسمهم اللي هو هذا ال .. بصير ونسميه + +587 +00:45:29,710 --> 00:45:36,340 +nasalbody و بعدين ال hook اللى هو شايفين هو ده + +588 +00:45:36,340 --> 00:45:40,800 +المنحني دماغ و بعدين من ال hook بتطلع اللى هو ال + +589 +00:45:40,800 --> 00:45:44,900 +filament الصورة + +590 +00:45:44,900 --> 00:45:47,180 +اللى شوفناها جاء و شوية عبارة عن مين عن ال + +591 +00:45:47,180 --> 00:45:50,200 +filament اللى فى الصورة اللى ظهر بينما ال hook و + +592 +00:45:50,200 --> 00:45:53,020 +ال basil body دجالة عشان نشوفهم فى ال bacteria + +593 +00:45:53,020 --> 00:46:00,920 +عموما نتخيل ال bacteria حجم كبير بالمقارنة مع مين + +594 +00:46:01,340 --> 00:46:05,560 +مع الفلاجلة هل يمكن رؤية الفلاجلة إذا احنا قادرين + +595 +00:46:05,560 --> 00:46:08,800 +نتكلم عن البكتيريا إنه بنشوفها تحت ال microscope + +596 +00:46:08,800 --> 00:46:15,340 +بنات شكل شحطة بهذا الشكل هل يمكن لنا رؤية الفلاجلة + +597 +00:46:15,340 --> 00:46:19,460 +اللي طالعة منها لأ لذلك إذا قررنا صباقة الفلاجلة + +598 +00:46:19,460 --> 00:46:23,980 +يجب أن نقوم بصباقة خاصة الصباقة الخاصة هذه معتمدة + +599 +00:46:23,980 --> 00:46:33,400 +على إيش على إنه نحط صبرة قادرة على الالترصب على + +600 +00:46:33,400 --> 00:46:36,260 +الفلاجلة specifically وبالتالي بتزيد من قطر من + +601 +00:46:36,260 --> 00:46:41,260 +الصبغة نفسها بتترصب على الفلاجلة فبتزيد من قطر + +602 +00:46:41,260 --> 00:46:45,540 +الفلاجلة وبالتالي يمكن ايضا او بيسميها special + +603 +00:46:45,540 --> 00:46:49,960 +flagella state انا + +604 +00:46:49,960 --> 00:46:55,260 +قولتلكوا انه فينا توزيع مختلف للفلاجلة لوحتنا هذه + +605 +00:46:55,260 --> 00:46:58,920 +الأخيرة في هذه المحاضرةهنتكلم على mono-trigas اللي + +606 +00:46:58,920 --> 00:47:03,460 +تلاحظه one single flagella فيه عندي amphitrigas + +607 +00:47:03,460 --> 00:47:09,960 +وهو على الطرفين تمام الانفي على الطرفين المفروض + +608 +00:47:09,960 --> 00:47:16,160 +انه مش ال .. مش بيه ال loafo اللي هو بيه loafo + +609 +00:47:16,160 --> 00:47:24,060 +يعني حزمة من ال flagella at one pole الاخيرة هي + +610 +00:47:24,060 --> 00:47:28,800 +الاخيرة اللي هي ال periodAround بيدي معناها around + +611 +00:47:28,800 --> 00:47:33,860 +فالفرجلة الاشاشة معمولة موزعة حول كل تلخلية إذا بس + +612 +00:47:33,860 --> 00:47:38,160 +الاختلاف هو إن أنا في الصورة بإن ال loaf هو بي و + +613 +00:47:38,160 --> 00:47:42,320 +الانفي هي ال سي لإن الانفي معناها متردد أو على + +614 +00:47:42,320 --> 00:47:45,600 +الطرفين ونختم إن شاء الله بهذه اللوحة ونكمل + +615 +00:47:45,600 --> 00:47:46,400 +المحاضرة القادمة + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/u5P7UmriOkc.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/u5P7UmriOkc.srt new file mode 100644 index 0000000000000000000000000000000000000000..c0d621992e08eac9b7863620b5ce65d843a0ac5b --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/u5P7UmriOkc.srt @@ -0,0 +1,2486 @@ +1 +00:00:21,580 --> 00:00:25,060 +بسم الله الرحمن الرحيم هنا المحاضرة الماضية توقفنا + +2 +00:00:25,060 --> 00:00:29,100 +عند أحد أعضاء الـ .. أو العضو الأساسي للحركة + +3 +00:00:29,100 --> 00:00:33,080 +البكتيريا اللي هو الفلاجلة واتفقنا على أنه مكون من + +4 +00:00:33,080 --> 00:00:36,060 +ثلاثة أجزاء رئيسية اللي هي الـ basal body أو الـ motor + +5 +00:00:36,060 --> 00:00:40,200 +اللي هو المحرك وكان embedded في الـ cell membrane + +6 +00:00:40,200 --> 00:00:44,760 +وكان في جزء اسمه الـ hook اللي هو خارج من الـ cell + +7 +00:00:44,760 --> 00:00:47,440 +membrane إلى خارج الخلية يعني مختلف عن الـ cell + +8 +00:00:47,440 --> 00:00:50,670 +wall وبعدين الـ filament اللي هو الخيط الطويل بعدين + +9 +00:00:50,670 --> 00:00:55,010 +تطرقنا لتوزيعات مختلفة لفلاجلة شفنا mono-trichas, + +10 +00:00:55,590 --> 00:00:59,690 +lovo-trichas, amphitrichas و peritrichas بحسب + +11 +00:00:59,690 --> 00:01:02,650 +توزيع الفلاجلة وقلنا إنه مهم جدا نعرف إن البكتيريا + +12 +00:01:02,650 --> 00:01:08,250 +متحركة أو غير متحركة لماذا هذه الأهمية في الـ + +13 +00:01:08,250 --> 00:01:12,970 +microbiology؟ نظرا نقول لكم أن عملية التعرف على + +14 +00:01:12,970 --> 00:01:15,750 +البكتيريا بتتم من خلال صفات إيجابية وصفات سلبية + +15 +00:01:15,750 --> 00:01:18,870 +فبنعطي مثال بسيط على هذا الموضوع الـ motility + +16 +00:01:18,870 --> 00:01:23,570 +بالذات، هناك Salmonella وهناك Shigella، اسمين + +17 +00:01:23,570 --> 00:01:26,230 +اقترنوا ببعض، لما بنقول Salmonella، غالبا على طول + +18 +00:01:26,230 --> 00:01:29,590 +وراها تلقائي بيجي موضوع الشيجيلا، Salmonella and + +19 +00:01:29,590 --> 00:01:36,160 +Shigella هذا بالارتباط الوثيق يعني .. يعني الشبه .. + +20 +00:01:36,160 --> 00:01:41,060 +الشبه بين الـ two organism أحد أهم الفروقات اللي + +21 +00:01:41,060 --> 00:01:43,940 +بنستخدمها بالتفريق بينهم الـ motility الـ salmonella + +22 +00:01:43,940 --> 00:01:48,190 +motile والـ shigella non-motile إذا الـ motility + +23 +00:01:48,190 --> 00:01:51,690 +بالنسبة لنا في الـ microbiology مهمة لأنها بتميزنا + +24 +00:01:51,690 --> 00:01:55,910 +بين البكتيريا نفسها إنها متحركة وغير متحركة طب + +25 +00:01:55,910 --> 00:01:58,870 +هنا كمان أهمية أخرى في البكتيريا المتحركة ما إحنا + +26 +00:01:58,870 --> 00:02:02,210 +شفنا قبل شوية في الرسم أو المحاضرة السابقة إنه في + +27 +00:02:02,210 --> 00:02:04,990 +بعض البكتيريا المتحركة بتكون متحركة بـ one single + +28 +00:02:04,990 --> 00:02:08,390 +polar flagella زي Pseudomonas aeruginosa وزي الـ + +29 +00:02:08,390 --> 00:02:13,030 +Vibrio cholera هاي مثل على اثنين بكتيريا مشهورة + +30 +00:02:13,030 --> 00:02:17,040 +بتتحرك بـ .. بـ flagella واحدة بينما الـ + +31 +00:02:17,040 --> 00:02:20,420 +Enterobacteriasi اللي بينها الإشريكية القولونية المفضلة + +32 +00:02:20,420 --> 00:02:25,100 +لِدَاكُم تتحرك بـ peritricous flagella إذن توزيع + +33 +00:02:25,100 --> 00:02:27,900 +الفلاجلة في البكتيريا أيضًا يساعدنا في التعرف على + +34 +00:02:27,900 --> 00:02:31,460 +نوع هذه البكتيريا إذن أول نقطة على اللوحة اللي + +35 +00:02:31,460 --> 00:02:35,360 +قدامكم إنه it is used in the identification of + +36 +00:02:35,360 --> 00:02:38,780 +بكتيريا تستخدم في التعريف بالنسبة للبكتيريا it + +37 +00:02:38,780 --> 00:02:44,000 +contributes to بعض الـ pathogenesis كيف اتفقنا إن هي تهرب + +38 +00:02:44,000 --> 00:02:44,300 +من +39 +00:02:47,030 --> 00:02:51,370 +المخاطر وتذهب إلى الغذاء وبالتالي هذا يعطيها أفضلية + +40 +00:02:51,370 --> 00:02:55,230 +على البكتيريا الغير متحركة طبعا إحنا قلنا إن هي الـ + +41 +00:02:55,230 --> 00:03:01,070 +organ لـ motility فيه البكتيريا نجي للتركيب الآخر + +42 +00:03:01,070 --> 00:03:07,690 +يسمى P-Lie الـ P-Lie عبارة عن hair-like projections + +43 +00:03:07,690 --> 00:03:08,990 +يعني زوائد تشبه + +44 +00:03:13,550 --> 00:03:16,510 +لأ مش الهداب، الهداب اسمها cilia والبكتيريا .. + +45 +00:03:16,510 --> 00:03:20,730 +والبكتيريا لا توجد بها cilia التراكيب الخاصة الصحيح هو + +46 +00:03:20,730 --> 00:03:23,730 +.. هنتعرف عليها إن شاء الله على صنفين منها الصنف + +47 +00:03:23,730 --> 00:03:27,690 +الأول بيسموه common pili أو fimbriae common pili + +48 +00:03:27,690 --> 00:03:31,650 +هي في قدامك وشايفينها هنا common pili أو fimbriae + +49 +00:03:31,650 --> 00:03:35,710 +وفينا الـ sex pili اللي هي الـ .. الـ pili المتعلقة + +50 +00:03:35,710 --> 00:03:38,730 +بالجنس الأولى اللي هي الـ common pili أو الـ + +51 +00:03:38,730 --> 00:03:42,750 +fimbriae عبارة عن فعن، فعن يعني إيه؟ رفيعة، rigid + +52 +00:03:43,560 --> 00:03:47,220 +adhesion rigid يعني فيها نوع من إيه؟ من الصلابة + +53 +00:03:47,220 --> 00:03:51,540 +numerous متعددة عديدة related to bacterial + +54 +00:03:51,540 --> 00:03:55,900 +adhesion هيتاني structure ندرسه إيه له علاقة + +55 +00:03:55,900 --> 00:04:01,040 +بالالتصاق أو الارتباط بالبكتيريا وهو الـ الـ الـ الـ + +56 +00:04:01,040 --> 00:04:04,160 +الـ بالغلاف الأول كان الـ capsule الأول كان الـ + +57 +00:04:04,160 --> 00:04:09,870 +capsule إذا الفيمبريا ضرورية جدا لعملية اللي هو الـ + +58 +00:04:09,870 --> 00:04:13,210 +attachment نجي للتانية اللي هو الـ sex pili وهي + +59 +00:04:13,210 --> 00:04:16,130 +عبارة عن longer أطول يعني في الـ .. في الـ .. في الـ + +60 +00:04:16,130 --> 00:04:21,210 +size و coarser .. coarser يعني أَخْمَر أسمك غالبا + +61 +00:04:21,210 --> 00:04:25,150 +بتكون موجودة بعدد قليل من واحد إلى أربعة وغالبا + +62 +00:04:25,150 --> 00:04:28,270 +الوظيفة الأساسية تبعها هو الـ conjugation الـ + +63 +00:04:28,270 --> 00:04:31,750 +conjugation عبارة عن اتصال جنسي ودعونا ننظر الآن + +64 +00:04:31,750 --> 00:04:39,860 +إلى animation بتوضح هذا التركيب في .. بشكل واضح قبل + +65 +00:04:39,860 --> 00:04:43,100 +ما نتكلم عن الـ conjugation خلينا نتكلم على اللي هو + +66 +00:04:43,100 --> 00:04:47,880 +الـ .. الـ .. الـ flushing away of بكتيريا اللي + +67 +00:04:47,880 --> 00:04:49,480 +مالهاش pili + +68 +00:04:56,340 --> 00:05:02,940 +طبعا هذا نفترض أنه هو الحاجز تمام الإنسان وإن + +69 +00:05:02,940 --> 00:05:07,000 +البكتيريا الآن صاعدة بدها تروح تعمل infection في + +70 +00:05:07,000 --> 00:05:11,840 +الكلى قطرة بول واحدة تعمل بتنزلهم لأن مافيش هنا + +71 +00:05:11,840 --> 00:05:15,500 +attachment للأسف هذه هي عبارة عن كرتون صورة متحركة + +72 +00:05:15,500 --> 00:05:19,820 +لكن بنظام المعين الموجود في الحاسوب هذا مش ظاهر + +73 +00:05:19,820 --> 00:05:24,140 +إنها animated هنزلكم يوم شير على الـ facebook و + +74 +00:05:24,140 --> 00:05:25,580 +تشوفوه الآن الصورة الثانية + +75 +00:05:32,930 --> 00:05:36,130 +بتوضح البكتيريا اللي عندها مقدرة على الـ attachment + +76 +00:05:36,130 --> 00:05:40,810 +بواسطة الفيمبريا أو الـ common pili حتى لو قطرة + +77 +00:05:40,810 --> 00:05:43,790 +البول اجت أو البول اجت بيبقى ثابتة في هذا + +78 +00:05:43,790 --> 00:05:47,450 +المكان، هذا الـ attachment بيقولنا أحد أهم العمليات + +79 +00:05:47,450 --> 00:05:50,790 +اللي بتساهم في الـ successful colonization في + +80 +00:05:50,790 --> 00:05:56,310 +البرنامج، لأن هحاول نشوف الـ conjugation + +81 +00:06:03,600 --> 00:06:08,540 +in conjugation DNA is transferred from one living + +82 +00:06:08,540 --> 00:06:13,140 +bacteria يبدو أن الصوت مش تمام خليني أتكلم على + +83 +00:06:13,140 --> 00:06:17,840 +اللوحة اللي أمامنا شايفين بكتيريا ترتبط ببكتيريا + +84 +00:06:17,840 --> 00:06:23,860 +أخرى صفاتهم مختلفين الآن الـ plasmid بدأت تنتقل + +85 +00:06:23,860 --> 00:06:29,610 +نسخة منها single وراحت للخلية الثانية الخلية + +86 +00:06:29,610 --> 00:06:35,510 +الثانية اكتسبت صفات الخلية الأولى وبالتمثيل إحنا + +87 +00:06:35,510 --> 00:06:41,850 +قد نستطيع أن نقول إن الأولى هي الـ male والثانية هي + +88 +00:06:41,850 --> 00:06:46,370 +الـ female لكن بعد اكتساب الصفة الـ female بتصير + +89 +00:06:46,370 --> 00:06:50,010 +male وبالتالي مافيش بالمفهوم اللي إحنا بنعرفه male + +90 +00:06:50,010 --> 00:06:54,110 +و female في البكتيريا وإنما وجود الصفة أنت تنتقل + +91 +00:06:54,110 --> 00:06:58,360 +إلى الخلية الأخرى فتكسبها هذه الصفة طبعا هذه + +92 +00:06:58,360 --> 00:07:01,700 +العملية راحظوا بتاعها بتعطي نسخة one single strand + +93 +00:07:01,700 --> 00:07:04,880 +بيصير له double strand هناك والأولى بتكون double + +94 +00:07:04,880 --> 00:07:09,900 +strand طبعا العملية قد بأكملها ممكن نمثلها بهذا + +95 +00:07:09,900 --> 00:07:16,180 +الشكل اللي أمامكواهذه المربعة F positive يعني فيها + +96 +00:07:16,180 --> 00:07:19,660 +صفة fertility ارتبطت بـ F negative الـ F negative + +97 +00:07:19,660 --> 00:07:23,980 +الآن على جهة وين الـ mouse بدأت الآن في التصاق وثيق + +98 +00:07:23,980 --> 00:07:27,280 +بينهم راح على المربعة الثالثة صار في عملية انتقال + +99 +00:07:27,280 --> 00:07:31,820 +للمادة الوراثية من الـ plasmid ومن ثم البكتيريا الـ F + +100 +00:07:31,820 --> 00:07:35,380 +negative صارت F positive وبدأت تويد هي الـ cycle + +101 +00:07:35,380 --> 00:07:40,460 +أخرى مع F negative cell هذه قضية normal conjugation + +102 +00:07:40,460 --> 00:07:44,000 +normal conjugation إن البلازميد هي اللي فقط بتنتقل + +103 +00:07:44,000 --> 00:07:48,980 +في نوع آخر من الـ conjugation بيسموه HFR high + +104 +00:07:48,980 --> 00:07:53,580 +frequency recombination هذه عملية رهيبة جدا + +105 +00:07:53,580 --> 00:07:57,760 +هنشوفها الآن يا عزيزي تتبولة كويس شايفين وين + +106 +00:07:57,760 --> 00:08:00,920 +البلازميد؟ مفيش البلازميد البلازميد وين موجودة؟ + +107 +00:08:00,920 --> 00:08:04,600 +البلازميد ارتبطت بالكروموزوم ارتبطت بمين؟ بالكروموزوم + +108 +00:08:05,290 --> 00:08:10,550 +ارتباطها بالكروموزوم يجعل من الكروموزوم كله أرضة + +109 +00:08:10,550 --> 00:08:17,170 +للانتقال إلى بكتيريا أخرى فتخيلوا .. تخيلوا .. أحد + +110 +00:08:17,170 --> 00:08:20,590 +من زملائكم دارس الكتاب كله .. الكتاب الـ micro + +111 +00:08:20,590 --> 00:08:25,930 +حافظه عن غيب .. من إيه؟ ويستطيع أن ينقل لكم كل ما + +112 +00:08:25,930 --> 00:08:29,670 +لديه من معلومات في الـ micro biology حتى تصحوا منه + +113 +00:08:29,670 --> 00:08:33,210 +ثاني يوم إيه؟ تحكم micro biology صح ولا مش صح؟ + +114 +00:08:33,790 --> 00:08:37,970 +فتخيلوا بكتيريا تنقل كروموزوم كامل منها إلى + +115 +00:08:37,970 --> 00:08:41,710 +بكتيريا أخرى، هتكسبها كل الـ experience والخبرة + +116 +00:08:41,710 --> 00:08:46,070 +الموجودة في معلوماتها المخزنة بالمادة الوراثية طب + +117 +00:08:46,070 --> 00:08:50,170 +هذا العمل كمان مرة، اقتلال، التصاد، بعدين بدينا في + +118 +00:08:50,170 --> 00:08:55,790 +الربع الثالث انتقال كامل أو جزء لكروموزوم تمام؟ + +119 +00:08:57,160 --> 00:09:02,700 +فصلنا هنا F positive وهكذا يبقى فيها إن العملية واضحة + +120 +00:09:02,700 --> 00:09:07,280 +أنه تقسم إلى نوعين النوع الأول لبلازميد فقط هي + +121 +00:09:07,280 --> 00:09:12,400 +اللي بتنتقل النوع الثاني لبلازميد زائد جزء أو كل + +122 +00:09:12,400 --> 00:09:16,180 +الكروموزوم جزء أو كل الكروموزوم على حسب إجدش + +123 +00:09:16,180 --> 00:09:20,560 +هوفرنا وقت للانتقال تعالوا نشوف الأخيرة في السلسلة + +124 +00:09:21,670 --> 00:09:26,950 +إحنا دائما أي شيء دائري بنحاول إنه نعمله للساعة + +125 +00:09:26,950 --> 00:09:29,770 +تعرف أنا تحت الـ microscope لو أنت تطلع تحت الـ + +126 +00:09:29,770 --> 00:09:33,970 +microscope و أنا شوفت شغل معين بدوريها لك بقول لك + +127 +00:09:33,970 --> 00:09:38,530 +تطلعي الساعة تلاتة بتطلع الساعة تلاتة أنتِ بتطلعي على + +128 +00:09:38,530 --> 00:09:42,250 +الدائرة تبع ال microscope ال field تمام و بتتخيلي + +129 +00:09:42,250 --> 00:09:46,990 +ساعة وين الساعة تلاتة على الجهة هذه تمام فبتخيلي لو + +130 +00:09:46,990 --> 00:09:50,470 +قلت لك الساعة 12 هتطلع أيه؟ فاضح لو قلت لك في المنتصف + +131 +00:09:50,470 --> 00:09:54,970 +لو قلت لك الساعة اثنتين يبقى نقدر نحدد اتجاهات + +132 +00:09:54,970 --> 00:09:59,050 +بموضوع الساعة فالساعة اللي أمامنا هذه بتوضح لنا + +133 +00:09:59,050 --> 00:10:03,350 +عملية نقل جيني لل chromosome تبقى أحد البكتيريا + +134 +00:10:03,350 --> 00:10:09,820 +بقول لنا الوقت المستقر لنقل كامل لكروموزوم من خلية إلى + +135 +00:10:09,820 --> 00:10:14,360 +أخرى قد يستغرق مائة دقيقة، مئات الدقائق، طبعاً مائة + +136 +00:10:14,360 --> 00:10:19,220 +دقيقة في الطبيعة غالباً لا توفر مائة دقيقة كاملة + +137 +00:10:19,220 --> 00:10:23,500 +تحت ثبات كامل، لازم يصير فيه disturbances، في + +138 +00:10:23,500 --> 00:10:26,820 +المختبر ممكن يوفر مائة دقيقة كاملة فينتقل + +139 +00:10:26,820 --> 00:10:28,940 +الكروموزوم، لكن في الطبيعة غالباً ما ينتقل الكروموزوم + +140 +00:10:28,940 --> 00:10:32,270 +كله، ينتقل أجزاء من الكروموزوم فبعطينا هنا هو + +141 +00:10:32,270 --> 00:10:36,030 +schematic presentation لكيفية الانتقال و كيف تأخذ + +142 +00:10:36,030 --> 00:10:39,790 +وقت، فبنضغط على فقط الجزء هذا، بدنا ننتقل يعني من + +143 +00:10:39,790 --> 00:10:45,910 +أول الساعة، اللي هي منطقة من؟ منطقة البلازميد، إلى + +144 +00:10:45,910 --> 00:10:50,230 +هذا الجين، بالمقابل على الجهة الثانية + +145 +00:10:54,580 --> 00:10:58,560 +وانتقل وانتقل في مين؟ وانتقل في الكروموزوم، ففي نوع من + +146 +00:10:58,560 --> 00:11:02,100 +combination فعلى الأقل البكتيريا حصلت على المعلومات + +147 +00:11:02,100 --> 00:11:05,260 +اللي في البلازميد وعلى جزء من مين؟ من الكروموزوم، + +148 +00:11:05,260 --> 00:11:10,020 +أعتقد أن الصورة واضحة أن ال six-ply لها وظيفة + +149 +00:11:10,020 --> 00:11:14,860 +محددة وهي ال conjugation أو نقل الجينات اللي قلنا + +150 +00:11:14,860 --> 00:11:18,100 +بتمثل إما بالبلازميد فقط لحالها في ال normal + +151 +00:11:18,100 --> 00:11:21,120 +conjugation أو البلازميد زي جزء من مين؟ من + +152 +00:11:21,120 --> 00:11:23,340 +الكروموزوم اللي سميناها high frequency + +153 +00:11:23,340 --> 00:11:24,260 +recombination + +154 +00:11:26,590 --> 00:11:33,050 +في شغل وفتحنا الـ .. الـ .. الـ .. الـ animation نرجع + +155 +00:11:33,050 --> 00:11:36,530 +برجعوها لـ cell membrane فاكرين ال cell membrane؟ + +156 +00:11:36,530 --> 00:11:41,730 +قلنا عنه phospholipid bilayer وهي بالفعل هي ال + +157 +00:11:41,730 --> 00:11:44,570 +phosphate وهي ال lipid تمام؟ وهي ال phosphate وهي + +158 +00:11:44,570 --> 00:11:48,650 +ال lipid وهذا عبارة عن bilayer خلينا نشتغل الـ + +159 +00:11:48,650 --> 00:11:55,400 +cell membrane of a prokaryotic cell بعطينا تصور عن + +160 +00:11:55,400 --> 00:11:57,520 +شكل ال membrane و التركيب تبعه + +161 +00:12:04,210 --> 00:12:08,390 +نوقف عند اللقطة هذه فكذا لما عملنا تمثيل احنا قلنا + +162 +00:12:08,390 --> 00:12:12,810 +أن ال membrane هو عبارة عن بحر من الدهون تسبح به + +163 +00:12:12,810 --> 00:12:16,210 +مجموعة من مين؟ من البروتينات، أيه البروتينات؟ أحد + +164 +00:12:16,210 --> 00:12:18,510 +البروتينات هو عبارة عن integral proteins + +165 +00:12:18,510 --> 00:12:21,070 +ال integral proteins لاحظوا طبعاً هي عبارة عن + +166 +00:12:21,070 --> 00:12:26,630 +channels يعني قنوات، القنوات المتخصصة واحنا قلنا + +167 +00:12:26,630 --> 00:12:31,070 +selective permeability فبالتالي هي اللي بتكون + +168 +00:12:31,070 --> 00:12:34,570 +بعملية تنظيم دخول وخروج الأيونات وبتختلف عن ال + +169 +00:12:34,570 --> 00:12:37,650 +peripheral أو ال integral proteins بحسب البكتيريا + +170 +00:12:37,650 --> 00:12:39,910 +وبحسب نوع ال membrane + +171 +00:12:47,490 --> 00:12:49,950 +طبعاً هذه البيئة الخارجية و هذه البيئة اللي هو + +172 +00:12:49,950 --> 00:12:53,610 +الداخلية اللي هي ممثلة ال cytoplasm باللاحظ أن + +173 +00:12:53,610 --> 00:12:58,350 +ما فيش مركبة تدخل هكذا لا المركبة تدخل باستئذان + +174 +00:12:58,350 --> 00:13:02,750 +بمعنى أنه عكسنا من selective permeability وبعض + +175 +00:13:02,750 --> 00:13:06,550 +الأحيان ندفع فلوس عكسنا ندخل اللي بيسموه active + +176 +00:13:06,550 --> 00:13:09,930 +transport active transport في بعض الأشياء بالـ simple + +177 +00:13:09,930 --> 00:13:14,810 +diffusion ندفع فلوس أقصد الفلوس اللي في ال ATP نعم + +178 +00:13:14,810 --> 00:13:18,700 +هي من ال ATP إن شاء الله هنزلكم برضه دول الـ .. الـ + +179 +00:13:18,700 --> 00:13:21,520 +.. ال animation على موقع الفيسبوك، أنا نزلت + +180 +00:13:21,520 --> 00:13:25,860 +بعضهم للطلاب، في عندي كمان شغل ثاني بدي أوريكم + +181 +00:13:25,860 --> 00:13:27,160 +أيه، هم اللي هم ال microscopy + +182 +00:13:34,690 --> 00:13:39,330 +احنا التلاتة متفقين على أن microscopes أساسيات + +183 +00:13:39,330 --> 00:13:43,550 +أو أساسية في تعاملنا في الميكروبات لماذا؟ لأنه + +184 +00:13:43,550 --> 00:13:49,450 +عندي نقطة ضعف نقطة ضعف هذه تتمثل في صغر حجم + +185 +00:13:49,450 --> 00:13:52,090 +الميكروبات أنا تكلمت أن ال power of resolution + +186 +00:13:52,090 --> 00:13:56,970 +تبتدئ أو غير قادرة على تمييز نقطتين من فاصلتين أصغر + +187 +00:13:56,970 --> 00:14:01,010 +من حد معين والبكتيريا أصغر من هذا الحد فيستلزمنا + +188 +00:14:01,010 --> 00:14:06,930 +أن يكون في عندي أداء لل magnification وطبعاً أحد + +189 +00:14:06,930 --> 00:14:09,410 +الأدوات هذه اللي هي ال light microscope ال common + +190 +00:14:09,410 --> 00:14:12,630 +light microscope وبعدين تطوّرنا صار فينا اللي هو + +191 +00:14:12,630 --> 00:14:15,330 +ال electron microscope وطبعاً فيه تطورات أخذوا + +192 +00:14:15,330 --> 00:14:18,550 +بالكم أعلى من ال electron microscope لأن نتكلم عن + +193 +00:14:18,550 --> 00:14:21,790 +ال nano technology فوصلنا مايكروسكوبات نقدر نشوف + +194 +00:14:21,790 --> 00:14:25,790 +فيها تقريباً ال atoms وبالتالي فينا دائماً باستمرار + +195 +00:14:25,790 --> 00:14:31,510 +لكن عندي مشكلة أخرى غير صغر الحجم أن البكتيريا .. + +196 +00:14:31,510 --> 00:14:35,270 +إذا بتشوفوا هنا في اللوحة في two fields، واحد غير + +197 +00:14:35,270 --> 00:14:39,250 +مصبوغ والثاني مصبوغ، في الغير مصبوغ البكتيريا يعني + +198 +00:14:39,250 --> 00:14:43,330 +بالكاد يمكن أن نطلعها، لماذا؟ لأنها طبيعتها شفافة، + +199 +00:14:43,330 --> 00:14:48,430 +طبيعتها شفافة فعشان أنا أخليها مرئية أن بتاع هو + +200 +00:14:48,430 --> 00:14:52,270 +زمان كان فيه دائماً يجيبوا حاجة كوميدية أن واحد + +201 +00:14:52,270 --> 00:14:56,470 +ماشي و بده يدخل مكان فيلاقي نفسه موصول من مين + +202 +00:14:56,470 --> 00:15:01,150 +بزجاج لأن الزجاج شفاف فاحنا مش قادرين أصلاً نميز أن + +203 +00:15:01,150 --> 00:15:04,910 +فيه زجاج فكانوا بيعملوا .. بيحطوا ملصق على الزجاج + +204 +00:15:04,910 --> 00:15:07,830 +مثلاً لأنه .. لأنه بيبين أنه في شغالة في هذا المكان + +205 +00:15:07,830 --> 00:15:12,350 +أو بيعملوا painting للزجاج فاحنا مضطرين ندهن + +206 +00:15:13,040 --> 00:15:16,480 +البكتيريا نصبغ البكتيريا تمام حتى أنا أستطيع أن أنا + +207 +00:15:16,480 --> 00:15:18,760 +أمدها ال visualizing إذا تغلبنا على المشكلتين + +208 +00:15:18,760 --> 00:15:23,060 +المشكلة صغر الحجم والمشكلة الثانية اللي هي ال + +209 +00:15:23,060 --> 00:15:25,740 +transparency أو الشفافية الأولى بال microscopes + +210 +00:15:25,740 --> 00:15:29,520 +والثانية بال styling وال styling أنا تحدثت أو + +211 +00:15:29,520 --> 00:15:33,860 +طرقت له فقط لغرض واحد أوضح الفرق بين ال silvota وال + +212 +00:15:33,860 --> 00:15:36,600 +gram-positive وال gram-negative لكن اليوم خلينا + +213 +00:15:36,600 --> 00:15:42,270 +نطرق له لو احنا يعني دخلنا فيه أو اقتربنا من الدخول + +214 +00:15:42,270 --> 00:15:45,710 +في هذا المجال وفي المعمل بالذات أول معاملة + +215 +00:15:45,710 --> 00:15:48,010 +هتأخذوها إن شاء الله حد هتكون عن بليستانيا + +216 +00:15:51,550 --> 00:15:54,650 +هذه ال microscopes في مقارنة بين ال light + +217 +00:15:54,650 --> 00:15:59,470 +microscope وال electron microscope تقريباً كفكرة + +218 +00:15:59,470 --> 00:16:04,390 +عامة ل structures واحد أنه بنضرب إلى عينا تبعتنا ب + +219 +00:16:04,390 --> 00:16:09,770 +light أو ب electrons وبعدين بنعمل magnification وبعدين + +220 +00:16:09,770 --> 00:16:13,470 +بعدين نستقبل الصورة لكن الأدوات هي طبعاً مختلفة + +221 +00:16:13,470 --> 00:16:20,620 +تمام نجي لل styling يُقسم ال Staining في + +222 +00:16:20,620 --> 00:16:22,960 +الميكروبيولوجي لثلاث أقسام Simple Staining, + +223 +00:16:23,480 --> 00:16:27,760 +Differential Staining و Special Stain تعالوا إن + +224 +00:16:27,760 --> 00:16:33,640 +دابل المسميات غالباً تنشأ من وصف Simple Stain لأنه + +225 +00:16:33,640 --> 00:16:38,640 +بيستخدم Only One Dye، Dye معناها صبغة، Dye معناها + +226 +00:16:38,640 --> 00:16:44,780 +صبغة ركزوا معايا بس شوية عشان الـ .. الـ .. الـ .. + +227 +00:16:44,780 --> 00:16:47,720 +ال principle of staining مرتبط باللي بتقوله الآن + +228 +00:16:49,550 --> 00:16:53,170 +البكتيريا كأي مخلوق كأي خلية في الدنيا فيها + +229 +00:16:53,170 --> 00:16:56,670 +مركبات والمركبات هذه بتكون مُكوّنة من جزيئات + +230 +00:16:56,670 --> 00:17:00,190 +والجزيئات من عفن من atoms وال atoms ممكن تكون + +231 +00:17:00,190 --> 00:17:03,830 +الشحنة السالبة هي الغالبة أو الشحنة الموجبة هي + +232 +00:17:03,830 --> 00:17:08,910 +الغالبة لكن المحصلة النهائية للسالب والموجب في + +233 +00:17:08,910 --> 00:17:14,350 +الخلية لصالح السالب بمعنى أن هي الشحنة العامة تبع + +234 +00:17:14,350 --> 00:17:19,370 +الخلية سالبة يعني عشان نصبغها لازم نحط عليها صبغة + +235 +00:17:19,370 --> 00:17:21,370 +أيش؟ موجبة .. موجبة .. موجبة .. موجبة .. موجبة .. + +236 +00:17:21,370 --> 00:17:22,530 +موجبة .. موجبة .. موجبة .. موجبة .. موجبة .. موجبة + +237 +00:17:22,530 --> 00:17:22,570 +.. موجبة .. موجبة .. موجبة .. موجبة .. موجبة .. + +238 +00:17:22,570 --> 00:17:28,630 +موجبة .. موجبة .. موجبة .. موجبة .. موجبة .. موجبة + +239 +00:17:28,630 --> 00:17:41,330 +.. موجبة .. موجبة .. موجبة .. موجبة .. موجبة + +240 +00:17:41,330 --> 00:17:48,790 +.. موجبة .. اللي هو الورق الخلفي إذا كنا نصبغ + +241 +00:17:48,790 --> 00:17:53,950 +بكتيريا سالبة حقيقية بدنا basic dye كلمة basic + +242 +00:17:53,950 --> 00:17:58,950 +يعني positively charged يعني الشحنة تبعتها موجبة + +243 +00:17:58,950 --> 00:18:03,390 +فالأسباب اللي مدكورة أنا أقولها تحت simple stains + +244 +00:18:03,390 --> 00:18:10,170 +زي crystal violet وسفرانين وميثيلين بلو كلها basic + +245 +00:18:10,170 --> 00:18:14,170 +dyes تبارك الله كلها هي ال crystal violet والسفرانين + +246 +00:18:14,170 --> 00:18:18,350 +والميثيلين بلو ما يميز ال crystal violet عشان + +247 +00:18:18,350 --> 00:18:21,210 +المثال إنه إذا صبغنا البكتيريا به أيش هيكون + +248 +00:18:21,210 --> 00:18:25,270 +لونها؟ violet إذا السفرانين صبغنا به هيكون لون + +249 +00:18:25,270 --> 00:18:30,130 +البكتيريا أحمر يعني بمعنى آخر البكتيريا ستتخذ نفس + +250 +00:18:30,130 --> 00:18:33,870 +لون الصبغة اللي تصبح فيها وسميناها simple لأنه هنا + +251 +00:18:33,870 --> 00:18:38,170 +كمان مرة بس أخدم the one single dye هل كونه simple + +252 +00:18:38,170 --> 00:18:45,030 +وبسيط؟ ليس ذو أهمية لأَن له أهمية طيب أنا مش كنت + +253 +00:18:45,030 --> 00:18:48,770 +صايم ما كنتش أتطرق للأهميات في the gram staining و + +254 +00:18:48,770 --> 00:18:51,970 +في the simple stain لكن لأَ بقى أسمعت لكل واحد منهم + +255 +00:18:51,970 --> 00:18:56,910 +على الأقل أهمية واحدة the simple stain في عين + +256 +00:18:56,910 --> 00:19:01,410 +مرضية ممكن يقوللي أنه في أو ما في بكتيريا طب هل + +257 +00:19:01,410 --> 00:19:06,450 +مهمة المعلومة؟ ما هي مرادية؟ يعني إيه مرادية + +258 +00:19:06,450 --> 00:19:09,390 +العينة؟ إذا كانت العينة .. إذا كانت العينة من .. + +259 +00:19:09,390 --> 00:19:11,510 +من .. من .. فاكرين العينات لما جسمناها في الإنسان؟ + +260 +00:19:11,510 --> 00:19:14,850 +قلنا الإنسان بينقسم إلى قسمين ستربتوكوكس و ستافيلوكوكس + +261 +00:19:14,850 --> 00:19:19,050 +إذا كانت العينة ستربتوكوكس و لجينا فيها بكتيريا فهذا + +262 +00:19:19,050 --> 00:19:23,550 +مؤشر على المرض عادة و وجود بكتيريا ينفي احتمالية + +263 +00:19:23,550 --> 00:19:27,090 +على قاله زيه ينفي احتمالية وجود المرض بس وجود + +264 +00:19:27,090 --> 00:19:30,870 +البكتيريا فهذا واحد رقم اثنين إذا أنا سبقت + +265 +00:19:30,870 --> 00:19:34,050 +البكتيريا و شوفت البكتيريا أنا أستطيع أن أقول إنها + +266 +00:19:34,050 --> 00:19:37,830 +بس الـdye أو الكوكسايد، يعني الشكل تبعها، يبقى + +267 +00:19:37,830 --> 00:19:42,050 +the simple stain أعطاني نتيجتين، الأولى الآن، presence + +268 +00:19:42,050 --> 00:19:45,170 +or absence of bacteria، فهذه نقطة كتير مهمة، ميّرت + +269 +00:19:45,170 --> 00:19:49,570 +بين فيه infection ولا فيه شيء، infection، وأعطاتني + +270 +00:19:49,570 --> 00:19:54,260 +شكل وترتيب الخلايا في البكتيريا كمؤشر على ممكن أن + +271 +00:19:54,260 --> 00:19:58,820 +يكون نوع البكتيريا المسببة إذا وجدت ستربتوكوكس وكوكاي + +272 +00:19:58,820 --> 00:20:02,540 +بقول ستربتوكوكس نيمونيا على سبيل المثال يبقى في + +273 +00:20:02,540 --> 00:20:06,640 +عندي أهمية للـsimple stain أنه بعطيني presence or + +274 +00:20:06,640 --> 00:20:10,140 +absence وفي نفس الوقت بعطيني شكل and arrangement + +275 +00:20:10,140 --> 00:20:16,100 +نيجي لـ the differential stain اسمع على النقطة هذه + +276 +00:20:16,100 --> 00:20:22,370 +عشان نعرف الفكرة اللي بنحكيها هي نقطة بتوضح فكرة ال + +277 +00:20:22,370 --> 00:20:26,290 +.. ال .. ال .. ال differential stain واتنين اشتغلوا + +278 +00:20:26,290 --> 00:20:31,450 +بحصانين فأخوه فاتفق و جاله بينا نميزهم مع بعض بينا + +279 +00:20:31,450 --> 00:20:35,110 +نميزهم مع بعض جاله أنت قص ال .. ال .. اليد اليمنى + +280 +00:20:35,110 --> 00:20:38,710 +و أنا بقص اليد اليسرى للحصان فاللون طلع من ال + +281 +00:20:38,710 --> 00:20:41,050 +spot اللي كل واحد جاله أنا جالساته يميني و أنت + +282 +00:20:41,050 --> 00:20:43,470 +جالساته شمالك اتجاه يمينك و يميني واحد إحنا جالسين + +283 +00:20:43,470 --> 00:20:46,930 +نفس الشيء قص رجله اليسرى و رجله اليمنى إيه المهم + +284 +00:20:46,930 --> 00:20:51,100 +في الآخر الحصانين قاعدوا بين الرجلين فنظر لبعض + +285 +00:20:51,100 --> 00:20:55,460 +بعد المحاولة فقال يا سيدي بسيطة هو ما هو حصانك أسوأ + +286 +00:20:55,460 --> 00:21:04,180 +بدون حصاني أبيض يعني اللطيف في الموضوع إيش هو؟ أنه + +287 +00:21:04,180 --> 00:21:07,460 +هل نحن أصلا بحاجة إلى تمييز بين اثنين مميزين عن + +288 +00:21:07,460 --> 00:21:11,680 +بعض؟ يبقى كان ما كانش فيه .. وهذا أنا هأخدوا بالكم + +289 +00:21:11,680 --> 00:21:16,540 +.. إلها أساس علمي عندنا دقيق جدا ممنوع نحاول .. + +290 +00:21:16,540 --> 00:21:19,500 +ممنوع حتى نحاول أن نميز بين الاثنين أصلا هم + +291 +00:21:19,500 --> 00:21:22,880 +مختلفين يعني .. يعني خلينا نقول ذكر وأنثى، هالأنثى + +292 +00:21:22,880 --> 00:21:25,880 +هقول هذا صفاته كده، وهذا صفاته كده، هم مميزين + +293 +00:21:25,880 --> 00:21:29,640 +أساسا في البكتيريا لما تكون البكتيريا the gram + +294 +00:21:29,640 --> 00:21:33,620 +positive أو the gram negative ويتشابه في صفات مختلفة، + +295 +00:21:33,620 --> 00:21:37,820 +مثلانقول البكتيريا هذه اسمها Staphylococcus على + +296 +00:21:37,820 --> 00:21:41,620 +سبيل المثال إشريكية قولونية و الإشريكية كولاي اللي + +297 +00:21:41,620 --> 00:21:46,880 +هي .. اللي هي the gram negative اه إشريكية قولونية، هل + +298 +00:21:46,880 --> 00:21:50,760 +هناك داعي أن نقول كيف بنميز بين الستربتوكوكس؟ طب ماهي دي + +299 +00:21:50,760 --> 00:21:55,430 +the gram قصدي، ماهي دي the gram؟ وصل الصورة فعملية ال + +300 +00:21:55,430 --> 00:22:01,070 +differentiation هي عملية أساسية في the microbiology + +301 +00:22:01,070 --> 00:22:05,850 +وبالتالي يجب علينا أن نقوم بكل ما هو ممكن لتمييز + +302 +00:22:05,850 --> 00:22:08,630 +بين أنواع المتشابه من الميكروبات أنا قلت + +303 +00:22:08,630 --> 00:22:12,130 +Salmonella و قلت Shigella ميّز منهم من صفة + +304 +00:22:12,130 --> 00:22:17,010 +الموتليل الآن نيجي لـ the gram stain the gram stain تقريبا قسم + +305 +00:22:17,010 --> 00:22:23,200 +البكتيريا في العالم إلى قسمين the gram positive و the gram negative + +306 +00:22:23,200 --> 00:22:25,900 +بالضبط في الجامعة الإسلامية لما إحنا دي نسجل في + +307 +00:22:25,900 --> 00:22:30,860 +الجامعة أخدت رقم أنت 2013، مظبوط؟ لو أخوك سجل، + +308 +00:22:30,860 --> 00:22:37,740 +بياخد 2013، ليش 2013 و 2013؟ الحاسوب بمجرد أن أحط + +309 +00:22:37,740 --> 00:22:42,180 +له رقم واحد، بيبطل يدور في قاعة البيرتبات، + +310 +00:22:42,180 --> 00:22:46,680 +الطالبات بيروح على الطلاب، فعملية الاستثناء موجودة + +311 +00:22:46,680 --> 00:22:51,070 +حتى في عملية تصنيف الكتب في عملية تصنيف الطلبة إلى + +312 +00:22:51,070 --> 00:22:56,750 +مستويات إلى .. إلى تخصصات فمهم جدا لـ the gram stain و + +313 +00:22:56,750 --> 00:23:00,390 +خلينا ناخد كيف the gram stain بينشغل كـ animation + +314 +00:23:00,390 --> 00:23:04,570 +قلنا إن نركز الصورة على ال .. ال .. ال bacteria + +315 +00:23:04,570 --> 00:23:08,570 +الآن ابتدينا نصبغهم بـ crystal violet ابتدينا نصرهم + +316 +00:23:08,570 --> 00:23:14,750 +violet عملنا ايودين و الايودين دوره المثبت للون + +317 +00:23:14,750 --> 00:23:20,370 +تشوفوا إن اللون غمق عملنا عملية غسيل بالماء تلاحظوا + +318 +00:23:20,370 --> 00:23:23,710 +اللون تنفرط أضفنا الآن the decolorizer اللي هو + +319 +00:23:23,710 --> 00:23:26,610 +الكحول الآن ال .. ال .. ال .. ال the gram negative + +320 +00:23:26,610 --> 00:23:28,930 +فقد اللون بقينا the gram positive محتوى اللون + +321 +00:23:28,930 --> 00:23:34,670 +صبرنا بـ Safranin تمام؟ الآن the Safranin إيش صار في + +322 +00:23:34,670 --> 00:23:41,700 +الخلايا؟ violet بعد ما حصلنا بيقولنا violet وهي + +323 +00:23:41,700 --> 00:23:45,160 +اللون الثاني لون أحمر إذا the gram negative و the gram + +324 +00:23:45,160 --> 00:23:48,900 +positive اللي قال the gram negative لـ the gram negative + +325 +00:23:48,900 --> 00:23:52,820 +بس اللي بيقولنا عنها pink و السبب في ذلك أنها فقدت + +326 +00:23:52,820 --> 00:23:57,940 +اللون بعد عملية اللي هو the decolorizer طيب ماذا قام + +327 +00:23:57,940 --> 00:24:04,300 +the gram stain بفعله؟ قام بأنه ميّز البكتيريا + +328 +00:24:07,390 --> 00:24:10,650 +طيب نفس الوقت اللي ميّز إحنا شوفنا تحت ال + +329 +00:24:10,650 --> 00:24:12,270 +microscope شوفنا البكتيريا + +330 +00:24:15,060 --> 00:24:18,740 +شوفناها هل هي Bacillus ولا هي Coxal يعني فوائد ال + +331 +00:24:18,740 --> 00:24:22,380 +simple stain اللي شوفناها the simple stain ووجود أو + +332 +00:24:22,380 --> 00:24:25,620 +عدم وجود البكتيريا برضه the gram stain بعطينا إياها + +333 +00:24:25,620 --> 00:24:29,080 +الفوائد هذه بالإضافة إلى كل فوائد the simple stain + +334 +00:24:29,080 --> 00:24:31,780 +أعطانا قدرة على التمييز إن أنا the gram positive ولا + +335 +00:24:31,780 --> 00:24:37,520 +the gram negative طبعا أعطيكوا فائدة لطيفة على أهمية + +336 +00:24:37,520 --> 00:24:39,780 +معرفة إن البكتيريا the gram positive ولا the gram negative + +337 +00:24:41,880 --> 00:24:46,320 +عملي في العيار بالـ Antibiotic معتمدة بدرجة كبيرة + +338 +00:24:46,320 --> 00:24:51,780 +على معرفتنا بتركيب الخلية، يعني مثلا لو the gram positive + +339 +00:24:51,780 --> 00:24:56,380 +حلاج البنسالينات والسفالوسبورينس والفانكومايسينز + +340 +00:24:56,380 --> 00:24:59,100 +أنا بذكر أسماء جاهزة للمضادات الحيوية، هذه + +341 +00:24:59,100 --> 00:25:06,200 +dedicated أكثر للـ the Gram Negative الـ Amino + +342 +00:25:06,200 --> 00:25:08,620 +Glycosides و أخرى + +343 +00:25:08,620 --> 00:25:27,940 +الانتيباتيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكيكي + +344 +00:25:30,140 --> 00:25:34,960 +تسموه صبغ مميز عندهم المفضل على التمييز بينما + +345 +00:25:34,960 --> 00:25:40,000 +الطرف الثالث اسمه specialists تاني و كلمة special + +346 +00:25:40,000 --> 00:25:43,860 +فاكريني لما درسنا التركيط بالخلية أحد التركيط بين + +347 +00:25:43,860 --> 00:25:48,380 +داخلي و خارجي و كنا essential و special ومن ال + +348 +00:25:48,380 --> 00:25:52,520 +special غالبا مش .. الخلية يعني بتجرب تعيش من + +349 +00:25:52,520 --> 00:25:55,980 +غيرها من ضمن الأشياء هذه اللي هو the capsule و ال + +350 +00:25:55,980 --> 00:25:59,700 +flagella ممكن the endospore اللي شايفينه هنا تحت + +351 +00:25:59,700 --> 00:26:03,880 +مين the endospore؟ تحت the differential stain لكن هي + +352 +00:26:03,880 --> 00:26:07,120 +بقعها special structure و قد نطلق عليها في + +353 +00:26:07,120 --> 00:26:09,920 +the microbiology أيضا كمال special لإن إحنا نسبقه + +354 +00:26:09,920 --> 00:26:15,680 +تركيب خاص تركيب خاص طيب عشان إحنا هنمر كمان شوية + +355 +00:26:15,680 --> 00:26:21,500 +على the spore فخلينا نتكلم عن the endospore تركزوا + +356 +00:26:21,500 --> 00:26:27,160 +معايا شوية لأن هذا موضوع مهم جدا اسمين في ال + +357 +00:26:27,160 --> 00:26:30,700 +microbiology بدنا نعرفهم من الآن هم اللي يلهم + +358 +00:26:30,700 --> 00:26:35,120 +علاقة بهذا التركيب اللي هو the endospore Bacillus + +359 +00:26:35,120 --> 00:26:37,340 +and Clostridium + +360 +00:26:41,910 --> 00:26:43,810 +هيمروا معانا في ال .. في ال .. في ال presentation + +361 +00:26:43,810 --> 00:26:48,330 +Bacillus ومرّوا معانا بمصطلح Bacillus Anthracis + +362 +00:26:48,330 --> 00:26:52,170 +الجمرة الخبيثة وكل كما عرفته جدًّا هذا + +363 +00:26:52,170 --> 00:26:58,930 +الميكروب خطير جدا لدرجة أنه هو أحد المخزونات اللي + +364 +00:26:58,930 --> 00:27:02,250 +موجودة في كثير من الدول اللي تمتلك قدرة على الحرب + +365 +00:27:02,250 --> 00:27:07,300 +البيولوجية وحرب الخليج الأولى أو التعتيلات الأولى + +366 +00:27:07,300 --> 00:27:13,140 +كان بحجة أن العراق تمتلك الجمرة الخبيثة ومئات + +367 +00:27:13,140 --> 00:27:18,300 +الأطنان أو مئات الآلاف من الأطنان يبقى مرض خطير أو + +368 +00:27:18,300 --> 00:27:22,140 +مايكروب خطير ويمتلك القدرة على تكوين الـ spore + +369 +00:27:22,140 --> 00:27:28,100 +بكتيريا أخرى اسمها Clostridium كلكم سمعتم بالتتانوس + +370 +00:27:28,100 --> 00:27:34,320 +لما واحد بيجرح مصمع أنا في غزة ست ولا سيد ومتعلم + +371 +00:27:34,320 --> 00:27:37,600 +مش متعلم بيقولوا أعطوه خدوه وادوه على عيادة أعطوه + +372 +00:27:37,600 --> 00:27:41,700 +إبر التتانوس صح؟ هي واقع الأمر إبر ضد التتانوس إبر + +373 +00:27:41,700 --> 00:27:46,240 +ضد التتانوس مش إبر التتانوس الكلسترديوم التتاني هي + +374 +00:27:46,240 --> 00:27:50,840 +المسبب للتتانوس ميكروب مرعب ميكروب مرعب لدرجة أن + +375 +00:27:50,840 --> 00:27:57,140 +كل الناس خايفة منه وبتالي تحضر منه اللي هو أفراد + +376 +00:27:57,140 --> 00:28:01,040 +آخرين مجرمين زيه Clostridium Perfringens تسمعوا + +377 +00:28:01,040 --> 00:28:06,280 +بالغرغرينة؟ قاس أصابع إجليه، بعدين تكل الخدع، + +378 +00:28:06,280 --> 00:28:11,860 +بعدين شوية، لما خلص، و احنا جازجزنا؟ الغرغرينة بسبب + +379 +00:28:11,860 --> 00:28:15,700 +Clostridium Perfringens طيب الميكروب تاعه، برضه + +380 +00:28:15,700 --> 00:28:21,500 +Clostridium Botulinum، تخيلوا؟ مجرد + +381 +00:28:21,500 --> 00:28:24,440 +تذوق، طعام، يحتوي على + +382 +00:28:51,730 --> 00:28:57,320 +استخدموه في العمليات تشبهوا تخيلوا نفس الtoxin + +383 +00:28:57,320 --> 00:28:59,640 +اللي بيتكلم عنه بيستخدموه في العملية التجميلية، + +384 +00:28:59,640 --> 00:29:03,300 +إيش عملية تجميلية؟ هو مش عملية تجميلية، هي عملية + +385 +00:29:03,300 --> 00:29:08,160 +تدميرية، لكن بيسموها عملية تجميلية، لما الإنسان + +386 +00:29:08,160 --> 00:29:12,120 +بيكبر زينا، بيصير إنه تجاعيد، فالناس اللي بيحبوش + +387 +00:29:12,120 --> 00:29:18,070 +يكبروا، بيقولك تجاعيد هذه يعني مش مناسبة لهالتجاعيد + +388 +00:29:18,070 --> 00:29:23,450 +منعش بتنشأ، بتنشأ غالبا من الstress والاجهاد + +389 +00:29:23,450 --> 00:29:28,950 +والعضلات بتتعب وكذا، فبيحقنوا + +390 +00:29:28,950 --> 00:29:34,370 +المنطقة اللي فيها التجاعيد بالBotox، سم مخفف جدا + +391 +00:29:34,370 --> 00:29:38,810 +جدا منها لأخر الجامعة، مخفف تمام؟ إيش بيعمل السم؟ + +392 +00:29:38,810 --> 00:29:43,210 +هذا بيعمل Paralysis، شلل للعضلات المنطقة اللي + +393 +00:29:43,210 --> 00:29:45,930 +بيحقنوا فيها، لما يصيب شرع .. يصيبوا شلل + +394 +00:29:45,930 --> 00:29:51,250 +الارتخائي، فبترتخي العضلات بتروح التجاعيد، طبعا + +395 +00:29:51,250 --> 00:29:55,010 +تأثيره في .. لإنه مخفف جدا، محدود في بعد ست شهور + +396 +00:29:55,010 --> 00:30:00,730 +بترجع التجاعيد مرة أخرى، لكن كلوستريديوم .. كلوستريديوم هو أقوى + +397 +00:30:00,730 --> 00:30:05,730 +ثوم معروف للإنسان على كوكب السبلاتيتلت مايكروبات + +398 +00:30:05,730 --> 00:30:09,590 +من صنف الكلوسترديوم شفتهم تيتانوس و بيرفرينجينز و + +399 +00:30:09,590 --> 00:30:13,250 +بوتلونيوم و مايكروب على الباسلس اللي هو الباسلس + +400 +00:30:13,250 --> 00:30:17,550 +أندراسيس اللي بينتج برضه توكسين و بيستخدم في الحرب + +401 +00:30:17,550 --> 00:30:21,330 +البيوروجية التنتين هذولة بيكونوا اندوسبورت + +402 +00:30:21,330 --> 00:30:24,270 +اندوسبورت اندوسبورت اندوسبورت اندوسبورت اندوسبورت + +403 +00:30:24,270 --> 00:30:24,610 +اندوسبورت اندوسبورت اندوسبورت اندوسبورت اندوسبورت + +404 +00:30:24,610 --> 00:30:26,410 +اندوسبورت اندوسبورت اندوسبورت اندوسبورت اندوسبورت + +405 +00:30:26,410 --> 00:30:32,350 +اندوسبورت اندوسبورت اندوسبورت + +406 +00:30:32,350 --> 00:30:32,670 +اند + +407 +00:30:41,680 --> 00:30:46,000 +المعنى تراكيب خاصة تمتلكها بعض الميكروبات لتحمي + +408 +00:30:46,000 --> 00:30:50,340 +نفسها من الظروف البيئية السيئة ماذا يعني بظروف + +409 +00:30:50,340 --> 00:30:55,330 +بيئية سيئة؟ حرارة مرتفعة، حرارة منخفضة، أكسجين + +410 +00:30:55,330 --> 00:30:59,210 +عالي، أكسجين منخفض، حتى لو كان فيش nutrient، يعني + +411 +00:30:59,210 --> 00:31:04,070 +فيش غذاء معين للبكتيريا، بدل ما تموت من الجوع، + +412 +00:31:04,070 --> 00:31:10,050 +تتطوق، بتتحوصل، تتجرثم، وبتكون اندو اسفل، تمام؟ + +413 +00:31:10,050 --> 00:31:12,910 +كمان مرة مش كل البكتيريا قادرة على هذا الفعل، only + +414 +00:31:12,910 --> 00:31:17,540 +two bacteriaو مادام only 2 بكتيريا يبقى مين مستهل + +415 +00:31:17,540 --> 00:31:20,560 +الواحد لو لدنا بكتيريا endospore-forming مميزها عن + +416 +00:31:20,560 --> 00:31:23,220 +باقي لإن و لا البكتيريا صح ولا لأ البتالي اللي + +417 +00:31:23,220 --> 00:31:26,160 +استخدمنا مين endospore stain طلع على ال endospore + +418 +00:31:26,160 --> 00:31:29,600 +stain نصبغ + +419 +00:31:29,600 --> 00:31:33,680 +البكتيريا بحسب الملاكاية جرين الملاكاية جرين + +420 +00:31:33,680 --> 00:31:37,400 +لحاله ممكن تدخل لازم نسخن هي عملنا عمالية تسخين + +421 +00:31:37,400 --> 00:31:43,340 +عمالية heating سخننا ال sport انصبغت رسلنا وضعنا + +422 +00:31:43,340 --> 00:31:49,940 +الآن صبغ مغاير اللي هو صفرانين أو Carbol Fuchsin بعض + +423 +00:31:49,940 --> 00:31:54,940 +الحالات اللي بيحطوا صفرانين حاطينها في الحالة إيش + +424 +00:31:54,940 --> 00:31:59,140 +صار في الخلايا بعد الغسيل ال sport صبغت باللون + +425 +00:31:59,140 --> 00:32:01,260 +الأخضر شايفين هذا اللون + +426 +00:32:04,380 --> 00:32:07,900 +إذا البكتيريا اللي فيها ال sport ال sport أخدت + +427 +00:32:07,900 --> 00:32:10,540 +اللون الأخضر اللون اللون أصل الملاكاية ال green + +428 +00:32:10,540 --> 00:32:16,320 +بينما الخلية المهترقة فقدت الملاكاية ال green و لما + +429 +00:32:16,320 --> 00:32:20,360 +صبغت مرة أخرى أخدت الصفرانين فالقاعد الخلية اللي + +430 +00:32:20,360 --> 00:32:24,600 +هو مصبوغة بلون أحمر و ال sport مش مصبوغة باللون + +431 +00:32:24,600 --> 00:32:27,020 +الأخضر لاحظوا الخلية اللي أصلا مش مكونة in the + +432 +00:32:27,020 --> 00:32:30,220 +sport مش فيها لون أخضر لمرة بينما اللي مكونة in + +433 +00:32:30,220 --> 00:32:35,580 +the sport مش فيها لون أخضر استطعنا أن نميز الاختصار + +434 +00:32:35,580 --> 00:32:40,060 +بين sport former و non sport former قبل أن تكون + +435 +00:32:40,060 --> 00:32:47,080 +الصورة واضحة بالنسبة للاختصارات الأخرى مثل كبسول + +436 +00:33:15,350 --> 00:33:19,770 +لاحظوا في النهاية التنين صبغوا البكتيريا انصبغت في + +437 +00:33:19,770 --> 00:33:24,210 +التنين تمام لأنه بدأنا ب basic stain بدأنا ب basic + +438 +00:33:24,210 --> 00:33:29,310 +stain بعدين لما صبغنا الخلفية بال أيوزين اللي + +439 +00:33:29,310 --> 00:33:34,030 +شايفينه قدامكم هالهالمنطقة اللي حوالين الخلايا + +440 +00:33:34,030 --> 00:33:37,110 +المكونة للكابسول لم تصبح لأن في أول مرة هو + +441 +00:33:37,110 --> 00:33:41,770 +negative stain فصبغ الخلفية بس لم يصبح اللي هو + +442 +00:33:41,770 --> 00:33:45,670 +الكابسول وبتالي صار في فراغ بين الخلية البكتيريا + +443 +00:33:45,670 --> 00:33:49,510 +وبين الصبغ المغاير اللي هو ال background وبتالي + +444 +00:33:49,510 --> 00:33:53,610 +صار في فراغ اللي شايفينه بهذا الشكل شايفينه الأبيض + +445 +00:33:57,810 --> 00:34:01,810 +الآن .. الآن .. هذا سمي special لأنه بي .. بي .. + +446 +00:34:01,810 --> 00:34:07,250 +بي .. بيستخدم لصباغة تراكيب خاصة لما ال endospore + +447 +00:34:07,250 --> 00:34:10,290 +كمان تراكيب خاصة وبالتالي بالفعل في بعض الكتب + +448 +00:34:10,290 --> 00:34:13,390 +الميكروفالية باللاجئ التصنيف ال endospore برضه تحت + +449 +00:34:13,390 --> 00:34:16,910 +مين؟ تحت اللي هو special stain نيجي للflagellar + +450 +00:34:16,910 --> 00:34:20,970 +stain ال + +451 +00:34:20,970 --> 00:34:26,310 +Proteus vulgaris المفروض أن كل التنين المفروض أنهم + +452 +00:34:26,310 --> 00:34:32,040 +..متحركين في خطأ كبير يبدو خلينا نشوف نهاية ال + +453 +00:34:32,040 --> 00:34:37,460 +animation كلمة multistep flagella restaining يعني + +454 +00:34:37,460 --> 00:34:42,780 +الخطوة تكرر مرارا وتكرارا ليش مرارا وتكرارا؟ + +455 +00:34:42,780 --> 00:34:47,720 +بدنا نزيد قطر الفلاجلة لاحظوا ده بلا تغلقها يظهر بعض + +456 +00:34:47,720 --> 00:34:52,760 +الصور بمعنى إنه صبغنا مرارا وتكرارا وغسلنا فبدنا + +457 +00:34:52,760 --> 00:34:57,300 +نشيل الصبغة عن البكتيريا بس بتضلها مترس برمان على + +458 +00:34:57,300 --> 00:35:02,140 +الفلاجللة كل مرة بنصبغ بنزيد قطر القليلة حتى يظهر + +459 +00:35:02,140 --> 00:35:06,440 +بهذا الشكل و زي ما توقعت إنه التنين هما بكتيريا + +460 +00:35:06,440 --> 00:35:10,260 +متحرك عليهم الفلاجللة والتصنيف تبعهم إيش هنا + +461 +00:35:10,260 --> 00:35:19,240 +polar ولا mono ولا peri ولا .. ما اسمه؟ بيري في + +462 +00:35:19,240 --> 00:35:22,640 +بيري تريكاس بيري تريكاس player + +463 +00:35:33,270 --> 00:35:36,790 +عودة إلى ال presentation تبعتنا واتكلمنا عن احنا + +464 +00:35:36,790 --> 00:35:41,150 +ال two functions ده ال pili وهنا ال pili الأولى + +465 +00:35:41,150 --> 00:35:46,050 +ال common pili أو الفمبريا وظيفتها + +466 +00:35:46,050 --> 00:35:50,250 +الأساسية اللي هو ال adhesion أو ال attachment أما + +467 +00:35:50,250 --> 00:35:54,350 +الثانية فكانت عبارة عن اللي هو ال contribution و + +468 +00:35:54,350 --> 00:35:57,130 +شرحنا ال six pili وال common pili بس ال common + +469 +00:35:57,130 --> 00:36:00,190 +pili في مثال عليها لازم نقوله في بكتيريا اسمها + +470 +00:36:00,190 --> 00:36:02,390 +Neisseria gonorrhoeae Neisseria gonorrhoeae + +471 +00:36:03,140 --> 00:36:07,940 +السيريجونورية هي عبارة عن STD STD يعني sexually + +472 +00:36:07,940 --> 00:36:14,380 +transmitted bacteria هنتقل عن طريق الجنس وهي ثاني + +473 +00:36:14,380 --> 00:36:20,420 +أكبر مسبب لـ ال STD في أوروبا وأمريكا بعد + +474 +00:36:20,420 --> 00:36:26,720 +الكلاميديا تتميز هذه البكتيريا بمقدرتها المهولة + +475 +00:36:26,720 --> 00:36:29,680 +على ال attachment عند المقدر عليها على + +476 +00:36:29,680 --> 00:36:34,890 +ال attachment على الأسطربتوريكم الآن نقطة كتير مهمة + +477 +00:36:34,890 --> 00:36:41,130 +لأن لو أنا يعني بنفكر بطريق المنطق أفضل وسيلة + +478 +00:36:41,130 --> 00:36:46,830 +نمنعها أن نكون أجسام مضادة ضد مين؟ ضد ال pili + +479 +00:36:46,830 --> 00:36:51,430 +فبالتالي نخلي الأجسام مضادة بترتبط بال pili ففي + +480 +00:36:51,430 --> 00:36:53,610 +هذه الحالة ال pili بتعتقد أنها بترتبط مين؟ في + +481 +00:36:53,610 --> 00:36:58,250 +الأسطح عندنا فبس إن أنا بعمل هيك بيكون أجسام مضادة + +482 +00:36:58,250 --> 00:37:03,880 +ضد ال pili إيش بتعمل هي بالمقابل؟ بتغير من + +483 +00:37:03,880 --> 00:37:08,720 +أنتجينات البيلائي، فاكرين الكلام اللي حكيناه على + +484 +00:37:08,720 --> 00:37:13,570 +مقدرة البكتيريا على ال antigenic variation؟ هذه + +485 +00:37:13,570 --> 00:37:16,930 +البكتيريا أحد أهم أسلحتها ال attachment، بدون + +486 +00:37:16,930 --> 00:37:20,190 +attachment تفقد القدرة، فلما شاهد إن الإنسان + +487 +00:37:20,190 --> 00:37:24,370 +بيكوّنلها أجسام مضادة ضد ال attachment، هي صارت + +488 +00:37:24,370 --> 00:37:27,110 +تغير من قدرتها على .. أو من ال .. من ال .. من ال + +489 +00:37:27,110 --> 00:37:30,670 +pili نفسها، عن جنية تبعيها، وبالتالي يصاب بها + +490 +00:37:30,670 --> 00:37:34,770 +الإنسان مرارا وتكرار، مرارا وتكرار، طبعا الناس + +491 +00:37:34,770 --> 00:37:40,540 +اللي بيرتكبوا الفحشة ال Endospore هو تركيب من + +492 +00:37:40,540 --> 00:37:44,560 +التراكيب المهم جدا وحاولنا نشرح عنها قبل قليل في + +493 +00:37:44,560 --> 00:37:47,680 +ال studying لكن لا بأس إنه نفترق المعلومات + +494 +00:37:47,680 --> 00:37:51,720 +الموجودة في اللوحة Endospores are highly resistant + +495 +00:37:51,720 --> 00:37:57,040 +resting كلمة resting يعني non-active يعني مش ناشطة + +496 +00:37:57,040 --> 00:38:01,280 +ال metabolism فيها يعني تقريبا مقدار zero + +497 +00:38:06,210 --> 00:38:11,290 +Produced within cells اللي بتنتجها الخلايا They + +498 +00:38:11,290 --> 00:38:14,690 +are common to organisms which live in soil and may + +499 +00:38:14,690 --> 00:38:18,350 +need to wait out some rough time such as ارتفاع + +500 +00:38:18,350 --> 00:38:22,310 +درجة الحرارة, radiation, drying chemical agents + +501 +00:38:22,310 --> 00:38:28,530 +تحت ظروف ملائمة a spore will germinate خذوا بالكم + +502 +00:38:28,530 --> 00:38:34,610 +من المسميات الخلية البكتيرية التي تتغذي و تتكاثر + +503 +00:38:34,610 --> 00:38:38,470 +يسموها Vegetative + +504 +00:38:38,470 --> 00:38:43,810 +Cell عندما تتحول إلى Spore نسمي العملية + +505 +00:38:43,810 --> 00:38:44,730 +Sporulation + +506 +00:38:47,350 --> 00:38:52,290 +عندما تتحول الـ spore إلى خلية vegetative نسميها + +507 +00:38:52,290 --> 00:38:55,810 +germination عارفين الـ germination بيطلق على من؟ + +508 +00:38:55,810 --> 00:38:59,810 +على النباتات البذور لما تنبت نقول عنها germinate + +509 +00:38:59,810 --> 00:39:05,490 +فبالتالي هي تشبه العملية التي هي الإنبات لأنه بذرة + +510 +00:39:05,490 --> 00:39:14,940 +تكون نبتة بذرة تكون خلية كمان مرة الخلية التي + +511 +00:39:14,940 --> 00:39:20,080 +تتكاثر تتغذي الـ vegetative cell عندما تتحول إلى + +512 +00:39:20,080 --> 00:39:24,320 +spore نسمي العملية Sporulation وعندما تتحول الـ + +513 +00:39:24,320 --> 00:39:27,020 +spore إلى خلية عادية التي هي vegetative cell + +514 +00:39:27,020 --> 00:39:32,400 +نسميها Germination عارفين + +515 +00:39:32,400 --> 00:39:38,720 +أنه في الكهرمان يحتفظ بحشرات من مئات البلايين من + +516 +00:39:38,720 --> 00:39:42,120 +السنين بعض العلماء جاءوا وطبعا الحشرات هذه ممكن + +517 +00:39:42,120 --> 00:39:48,060 +يكون فيها spores تعالوا نفتح الكهرمان هذا ونشوف + +518 +00:39:48,060 --> 00:39:51,260 +الحشرة التي محفوظة بلايين السنين أو مئات الآلاف + +519 +00:39:51,260 --> 00:39:55,880 +السنين ونشوف إذا كان يطلع منها بكتيريا وفي يعني + +520 +00:39:55,880 --> 00:39:59,160 +كانت تقارير تقول إنه استطاع العلماء أن يستنبطوا + +521 +00:39:59,160 --> 00:40:02,860 +الجراثيم التي كانت محفوظة من بلايين السنين وهناك + +522 +00:40:02,860 --> 00:40:06,800 +أيضا كمان أخبار تقول إن الفراعنة برضه في جدتهم + +523 +00:40:06,800 --> 00:40:11,790 +لقوا spores واستنبطوها مرة أخرى معنى الحكي أن الـ + +524 +00:40:11,790 --> 00:40:16,990 +spores قادرة على الـ survival إلى سنوات عديدة تقتصر + +525 +00:40:16,990 --> 00:40:23,390 +إلى مئات الآلاف من السنة الـ spores التي قلنا الـ + +526 +00:40:23,390 --> 00:40:26,390 +genesis التانين التي هم الـ Bacillus والـ Clostridium + +527 +00:40:26,390 --> 00:40:29,770 +في الآخر اللوحة التي أمامنا الآن الصورة التي + +528 +00:40:29,770 --> 00:40:34,010 +أمامكم توضح الـ location of الـ spore location + +529 +00:40:34,010 --> 00:40:39,550 +location احنا اتفقنا أنه فيه أو ما فيه spore صح؟ و + +530 +00:40:39,550 --> 00:40:43,210 +بس تنتهي التي عندنا وكليسترديوم وبصلص، هي مبنية + +531 +00:40:43,210 --> 00:40:48,110 +على الـ spore يعني، تمام؟ الآن الشكل الذي أمامكم + +532 +00:40:48,110 --> 00:40:52,370 +يوضح لاحظوا الـ spore هنا والتي موجودة في المنتصف + +533 +00:40:52,370 --> 00:40:56,810 +خارج الحدود ولا دامونه حدود الخلية طب هي في + +534 +00:40:56,810 --> 00:41:02,010 +المنتصف لكن عاملة bulging خارج حدود الخلية في + +535 +00:41:02,010 --> 00:41:04,690 +اختلاف ولا مش اختلاف إذا بتكلمنا عن الـ location + +536 +00:41:04,690 --> 00:41:09,130 +central أنا عندي terminal طب هنا terminal هنا + +537 +00:41:09,130 --> 00:41:15,190 +terminal هنا terminal oval وهي الـ terminal هو مش + +538 +00:41:15,190 --> 00:41:23,810 +circle لأنها كروية لأنها كروية لكن صار عندي location + +539 +00:41:23,810 --> 00:41:29,590 +صار عندي morphology شكل يعني يوضح لي أنها oval + +540 +00:41:29,590 --> 00:41:33,070 +ولا spherical هل هي within the boundaries of the + +541 +00:41:33,070 --> 00:41:37,630 +cell ولا bulging يعني خارج أطاق الـ cells طيب هذا + +542 +00:41:37,630 --> 00:41:41,980 +المعلومات تفيدنا ولا متفادنها ايش؟ تفيدنا في .. و + +543 +00:41:41,980 --> 00:41:44,860 +هذا الكلام لما ندرس الـ diagnostic microbiology، + +544 +00:41:44,860 --> 00:41:48,140 +ندرس مثلا ونقول لكم Bacillus anthracis، نقول لكم + +545 +00:41:48,140 --> 00:41:51,480 +أن الـ spore في الـ Bacillus anthracis is centrally + +546 +00:41:51,480 --> 00:41:54,960 +located، هذه أصبحت معلومة على الهامش، أصبحت معلومة + +547 +00:41:54,960 --> 00:41:58,620 +ضمن المعلومات المستخدمة في عملية التعرف على + +548 +00:41:58,620 --> 00:42:02,380 +البكتيريا نفسها، أرجو الانتباه إلى هذه القضية + +549 +00:42:05,650 --> 00:42:16,690 +فيه انحاسمة Dipicronic Acid أو باختصار DAP الـ + +550 +00:42:16,690 --> 00:42:21,730 +Dipicronic Acid موجود + +551 +00:42:21,730 --> 00:42:30,610 +في البكتيريا بكميات نادرة جدا يعني كميات قليلة جدا + +552 +00:42:30,610 --> 00:42:38,280 +لكن في الـ spore بعشرات المرات من تركيزه في أين؟ في + +553 +00:42:38,280 --> 00:42:43,740 +الخلية العادية لأنه ارتبط بالـ spore كميته كثر في + +554 +00:42:43,740 --> 00:42:46,680 +الـ spore فهناك اعتقاد أنه + +555 +00:42:50,940 --> 00:42:56,640 +وهو المسؤول عن توفير الـ protection للبكتيريا بشكل + +556 +00:42:56,640 --> 00:42:59,400 +كبير Spores that were dormant for thousands of + +557 +00:42:59,400 --> 00:43:03,600 +years in the great tombs of the Egyptian pharaohs + +558 +00:43:03,600 --> 00:43:08,320 +الذين كانوا قادرين على ترميلها وتنفجرها عندما + +559 +00:43:08,320 --> 00:43:12,780 +كانوا موضعينها في مصطلحات مناسبة هنا مرة أخرى + +560 +00:43:12,780 --> 00:43:16,780 +There are even claims هناك دعاوى + +561 +00:43:20,130 --> 00:43:25,010 +over 250 million years old being able to generate + +562 +00:43:25,010 --> 00:43:28,370 +when placed in appropriate means نفس المعلومة التي + +563 +00:43:28,370 --> 00:43:33,530 +ذكرت أنه جراثيم عمرها أكثر من 250 مليون سنة، هناك + +564 +00:43:33,530 --> 00:43:39,080 +ادعاءات التي تم استنباطها لموضوع مرة أخرى الجملة + +565 +00:43:39,080 --> 00:43:42,840 +الأخيرة كوننا نؤمن بعلميين نقول these + +566 +00:43:42,840 --> 00:43:46,700 +results have yet to be validated هذه النتائج يجب + +567 +00:43:46,700 --> 00:43:55,900 +أن يتحقق منها يعني مش معلومات مؤكدة مسلمة طيب + +568 +00:43:55,900 --> 00:43:59,340 +نتحدث لكم بس يعني كيف تكون الـ spores أو طريقة + +569 +00:43:59,340 --> 00:44:01,500 +تكوين الـ spore عشان برضه تكون الصورة واضحة عندي + +570 +00:44:03,450 --> 00:44:07,110 +نسمع كلمة spore في مكانين مختلفين، في البكتيريا + +571 +00:44:07,110 --> 00:44:12,530 +وفي الفطريات المفهوم للـ spore في البكتيريا يختلف + +572 +00:44:12,530 --> 00:44:17,070 +تماما عن الـ spore في الفطريات الـ spore في الفطريات + +573 +00:44:17,070 --> 00:44:23,130 +هي أداة من أدوات التكاثر، الخلية الواحدة تنتج + +574 +00:44:23,130 --> 00:44:28,170 +ملايين الـ spores ملايين lesbois أعتقد كلكم في + +575 +00:44:28,170 --> 00:44:34,190 +المطبخ عفا عندكم شيء حبة برتقال أو فماطن أو حتى + +576 +00:44:34,190 --> 00:44:38,590 +خبز وتلاقوا عليه عفا أطرافه وتكون بيضاء اللون + +577 +00:44:38,590 --> 00:44:43,570 +وفي المتصفر بيكون أسود أو أخضر أو أزرق أو أصفر لو + +578 +00:44:43,570 --> 00:44:47,810 +طلعتوا فنجلة من القهوة على المجلة لمدة مثلا أسبوع + +579 +00:44:47,810 --> 00:44:50,670 +أو أسبوعين طلعتوا عليها هتلاقوا بالفعل cottony + +580 +00:44:50,670 --> 00:44:53,910 +white وفي المتصفر فيه أنها greenish discolored لون + +581 +00:44:53,910 --> 00:45:00,710 +أخضر هذا عبارة عن fungus mold هذا الـ mold غالبا + +582 +00:45:00,710 --> 00:45:04,590 +بيكون له أبيض cottony white لكن اللون الذي أنتم + +583 +00:45:04,590 --> 00:45:08,170 +شايفينه الأخضر أو الأسود أو الأزرق أو الأصفر هو + +584 +00:45:08,170 --> 00:45:12,790 +لون الـ spores التي لو هذا الذي في الفنجان الصغير + +585 +00:45:12,790 --> 00:45:17,500 +أخذناه من عندهم بيلاقواهم بلايين الـ spores تستخدم + +586 +00:45:17,500 --> 00:45:21,580 +للتكاثر، تستخدم لا للتكاثر وليس للحماية، بينما + +587 +00:45:21,580 --> 00:45:26,440 +البكتيريا تستخدم لمن؟ للحماية، ما تستخدمش + +588 +00:45:26,440 --> 00:45:29,540 +للتكاثر، خلية واحدة تتجرث بيصير one spore، الـ one + +589 +00:45:29,540 --> 00:45:35,040 +spore بيصير germination، تمام؟ بترجع خلية واحدة، + +590 +00:45:35,040 --> 00:45:38,360 +فخلية، spore، خلية، مش تكاثر، ما فيش تكاثر + +591 +00:45:42,680 --> 00:45:46,000 +هي عبارة عن multiple membranes، لاحظوا السماكة + +592 +00:45:46,000 --> 00:45:49,360 +التابعة لها، هذه الـ cell wall، تمام، هذه الـ cell wall + +593 +00:45:49,360 --> 00:45:53,260 +تبع المادة العالية، وهي الـ cell wall تبع الـ + +594 +00:45:53,260 --> 00:45:58,300 +spores، طلعتوا أجدش السماكة التابعة له، تعرفوا الجربان، + +595 +00:45:58,300 --> 00:46:01,940 +كيف بتضبوه في الخزانة؟ مش بتحطوه وبتجلبوه و + +596 +00:46:01,940 --> 00:46:06,020 +بترجعوه نفس الفكرة بيتكون للـ spores بيسموها + +597 +00:46:06,020 --> 00:46:09,320 +phagocytase phagocyte هذه تأكل هذه وهذه تأكل + +598 +00:46:09,320 --> 00:46:13,100 +هذه بمعنى أخذ الـ cell membrane بيصير يكون طبقات و + +599 +00:46:13,100 --> 00:46:17,860 +بيراكم بيبتدوا جليكان وبيراكم D-dipicolinic acid D + +600 +00:46:17,860 --> 00:46:23,850 +-dipicolinic acid فيصير عندي تركيب ذو سماكة عالية و + +601 +00:46:23,850 --> 00:46:27,090 +تركيب خاص من الـ cell wall قادر على منع دخول + +602 +00:46:27,090 --> 00:46:30,870 +الرطوبة وداخل دخول الـ chemicals حتى الـ radiation + +603 +00:46:30,870 --> 00:46:34,150 +الـ ultraviolet light التي تقتل البكتيريا العالية + +604 +00:46:34,150 --> 00:46:38,350 +غير قادر على قتل البكتيريا التي لها indoor asthma + +605 +00:46:38,350 --> 00:46:46,410 +شايفين الجداش في عندي تركيب داخل الذي هو الـ spores + +606 +00:46:46,410 --> 00:46:50,750 +هذه الخلية وهي الـ spores تبقى تحتها هذه التي + +607 +00:46:50,750 --> 00:46:54,690 +شايفينها في جدامكم هذه هذا الشكل الـ typical + +608 +00:46:54,690 --> 00:47:02,570 +النموذجي لكلسترديوم تيتانيه عرض المصماري هذا + +609 +00:47:02,570 --> 00:47:06,810 +الخلية وهي + +610 +00:47:06,810 --> 00:47:10,510 +الـ spore الـ spore أين جاي على الطرف نسميها + +611 +00:47:10,510 --> 00:47:15,670 +terminal مش فيه كانت هنا central احنا يوجد على + +612 +00:47:15,670 --> 00:47:19,310 +الطرف نسميها subterminal وعلى الطرف نسميها + +613 +00:47:19,310 --> 00:47:20,010 +terminal + +614 +00:47:24,550 --> 00:47:26,790 +الـ microscope احنا أظن أننا مرينا عليهم في الـ + +615 +00:47:26,790 --> 00:47:30,030 +animation مش هنتكلم عن طريقهم كل الذي .. الذي .. + +616 +00:47:30,030 --> 00:47:32,850 +الذي .. الذي انذكر الآن هنا simple staining الـ + +617 +00:47:32,850 --> 00:47:35,070 +differential و الـ special staining كل ما دول مرينا + +618 +00:47:35,070 --> 00:47:38,010 +عليهم فداك احنا بنكون أن هنا الجزء المتعلق بالـ + +619 +00:47:38,010 --> 00:47:40,610 +bacterial cell structure وإن شاء الله يكون + +620 +00:47:40,610 --> 00:47:44,450 +المحاضرة القادمة عن الـ bacterial testulary الموضوع + +621 +00:47:44,450 --> 00:47:47,810 +برضه interesting وإن شاء الله يكون مفيد لنا + +622 +00:47:47,810 --> 00:47:48,530 +السلام عليكم diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/u5P7UmriOkc_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/u5P7UmriOkc_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..ffcfd8aa9d3e44139888da384f1f26bc9d143a43 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/u5P7UmriOkc_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 4930, "start": 21.58, "end": 49.3, "text": "بسم الله الرحمن الرحيم هنا المحاضرة الماضي توقفنا عند أحد أعضاء ال .. أو العضو الأساسي للحركة البكتيريا اللي هو 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staphylococcus على سبيل المثال إشليكوز فيرمنتا والإشريشيا كولاي اللي هي .. اللي هي gram negative اه إشليكوز فيرمنتا، هل هناك ده أنه نقول كيف بنميز بين التنتة؟ طب ماهي دي gram قصدي، ماهي دي gram؟", "tokens": [1863, 39648, 29739, 4117, 2655, 13546, 25528, 29538, 24525, 2304, 11296, 27284, 3495, 5842, 905, 1149, 15844, 8608, 21292, 1211, 9673, 12984, 6027, 11933, 8592, 20292, 4117, 2407, 11622, 6156, 13546, 27842, 2655, 995, 16070, 28814, 8592, 16572, 8592, 25528, 9122, 12610, 47302, 13672, 1829, 39896, 4386, 13672, 1829, 39896, 21353, 3671, 1975, 3224, 11933, 8592, 20292, 4117, 2407, 11622, 6156, 13546, 27842, 2655, 995, 12399, 8032, 1211, 34105, 4117, 11778, 3224, 14739, 3224, 8717, 39648, 9122, 33911, 44945, 2304, 1829, 11622, 49374, 16712, 29399, 3660, 22807, 23032, 3555, 19446, 3224, 1829, 11778, 1829, 21353, 12174, 9381, 16254, 12399, 19446, 3224, 1829, 11778, 1829, 21353, 22807], "avg_logprob": -0.3139602686757239, "compression_ratio": 1.6774193548387097, "no_speech_prob": 0.0, 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39.84, "end": 39.94, "word": " ما", "probability": 0.9443359375}, {"start": 39.94, "end": 40.42, "word": " يسمى", "probability": 0.9685872395833334}, {"start": 40.42, "end": 40.62, "word": " بال", "probability": 0.88916015625}, {"start": 40.62, "end": 40.98, "word": " pass", "probability": 0.3115234375}, {"start": 40.98, "end": 41.72, "word": " producing", "probability": 0.533203125}, {"start": 41.72, "end": 42.44, "word": " infection", "probability": 0.88037109375}], "temperature": 1.0}, {"id": 2, "seek": 7283, "start": 44.11, "end": 72.83, "text": "اطلعنا على بعض الصور اللى بتوضح الأشكال المختلفة اللى هندرسها تحت هذا الموضوع شفنا شكلها رئيسيين اللى هم ال chains و ال clusters ال chains و ال clusters في اللوحات اللى أمامكوا معظمهم ل ال strip to koukai فقط الوحيدة اللى منهم استفى ل koukai اللى على يمين الشاشة في الأعلى", "tokens": [41193, 1211, 3615, 8315, 15844, 45030, 11242, 31767, 13063, 13672, 7578, 39894, 2407, 11242, 5016, 16247, 8592, 4117, 6027, 9673, 46456, 46538, 3660, 13672, 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" الصور", "probability": 0.989990234375}, {"start": 45.49, "end": 46.09, "word": " اللى", "probability": 0.818603515625}, {"start": 46.09, "end": 46.83, "word": " بتوضح", "probability": 0.945556640625}, {"start": 46.83, "end": 48.71, "word": " الأشكال", "probability": 0.874267578125}, {"start": 48.71, "end": 49.51, "word": " المختلفة", "probability": 0.992919921875}, {"start": 49.51, "end": 50.27, "word": " اللى", "probability": 0.9921875}, {"start": 50.27, "end": 50.73, "word": " هندرسها", "probability": 0.73818359375}, {"start": 50.73, "end": 50.87, "word": " تحت", "probability": 0.9873046875}, {"start": 50.87, "end": 51.03, "word": " هذا", "probability": 0.90576171875}, {"start": 51.03, "end": 51.57, "word": " الموضوع", "probability": 0.9986572265625}, {"start": 51.57, "end": 52.69, "word": " شفنا", "probability": 0.786376953125}, {"start": 52.69, "end": 53.03, "word": " شكلها", "probability": 0.9072265625}, {"start": 53.03, "end": 53.81, "word": " رئيسيين", "probability": 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"probability": 0.62451171875}, {"start": 60.61, "end": 62.73, "word": " اللوحات", "probability": 0.9483642578125}, {"start": 62.73, "end": 62.83, "word": " اللى", "probability": 0.958984375}, {"start": 62.83, "end": 63.45, "word": " أمامكوا", "probability": 0.82509765625}, {"start": 63.45, "end": 64.67, "word": " معظمهم", "probability": 0.9326171875}, {"start": 64.67, "end": 64.93, "word": " ل", "probability": 0.92138671875}, {"start": 64.93, "end": 65.17, "word": " ال", "probability": 0.1475830078125}, {"start": 65.17, "end": 65.39, "word": " strip", "probability": 0.4755859375}, {"start": 65.39, "end": 65.55, "word": " to", "probability": 0.1669921875}, {"start": 65.55, "end": 66.01, "word": " koukai", "probability": 0.6497802734375}, {"start": 66.01, "end": 66.77, "word": " فقط", "probability": 0.994384765625}, {"start": 66.77, "end": 67.23, "word": " الوحيدة", "probability": 0.90224609375}, {"start": 67.23, "end": 67.33, "word": " اللى", "probability": 0.96923828125}, {"start": 67.33, "end": 67.67, "word": " منهم", "probability": 0.97998046875}, {"start": 67.67, "end": 68.21, "word": " استفى", "probability": 0.60791015625}, {"start": 68.21, "end": 68.33, "word": " ل", "probability": 0.92236328125}, {"start": 68.33, "end": 68.77, "word": " koukai", "probability": 0.85577392578125}, {"start": 68.77, "end": 69.15, "word": " اللى", "probability": 0.982666015625}, {"start": 69.15, "end": 70.13, "word": " على", "probability": 0.402099609375}, {"start": 70.13, "end": 70.95, "word": " يمين", "probability": 0.92919921875}, {"start": 70.95, "end": 72.35, "word": " الشاشة", "probability": 0.9884440104166666}, {"start": 72.35, "end": 72.47, "word": " في", "probability": 0.55029296875}, {"start": 72.47, "end": 72.83, "word": " الأعلى", "probability": 0.9820963541666666}], "temperature": 1.0}, {"id": 3, "seek": 10556, "start": 78.42, "end": 105.56, "text": "وبدأنا بالحديثة عن الـ Staphylococci والـ Staphylococci هي المجموع الأهم حقيقة من حيث عدد ال infection دي اللي بتسببها كلمة عدد يعني بتتكلم فيها frequency frequency يعني نسبة الإصابة كم مرة بيوصب البشر بهذا ال micro لأنه هتكلم عن ال Staphylococci برضه بأكتر لكن أكتر من ناحية الأنواع وليس ال frequency أنواع ال infection ال Staphylococci", "tokens": [37746, 3215, 10721, 8315, 20666, 5016, 16254, 12984, 3660, 18871, 2423, 39184, 745, 569, 3495, 5842, 905, 537, 4032, 6027, 39184, 745, 569, 3495, 5842, 905, 537, 39896, 9673, 7435, 2304, 45367, 16247, 16095, 11331, 38436, 28671, 9154, 11331, 1829, 12984, 6225, 3215, 3215, 2423, 11764, 11778, 1829, 13672, 1829, 39894, 35457, 3555, 11296, 9122, 19528, 3660, 6225, 3215, 3215, 37495, 22653, 39894, 2655, 4117, 19528, 8978, 11296, 7893, 7893, 37495, 22653, 8717, 35457, 3660, 33688, 9381, 16758, 3660, 9122, 2304, 3714, 25720, 4724, 1829, 2407, 9381, 3555, 29739, 46309, 39627, 15730, 2423, 4532, 5296, 33456, 3224, 8032, 2655, 4117, 19528, 18871, 2423, 745, 569, 3495, 5842, 905, 537, 4724, 43042, 3224, 4724, 10721, 4117, 2655, 2288, 44381, 5551, 4117, 2655, 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{"start": 87.72, "end": 87.96, "word": " يعني", "probability": 0.952392578125}, {"start": 87.96, "end": 88.52, "word": " بتتكلم", "probability": 0.7464599609375}, {"start": 88.52, "end": 88.92, "word": " فيها", "probability": 0.962646484375}, {"start": 88.92, "end": 89.66, "word": " frequency", "probability": 0.94482421875}, {"start": 89.66, "end": 90.36, "word": " frequency", "probability": 0.3828125}, {"start": 90.36, "end": 90.64, "word": " يعني", "probability": 0.979248046875}, {"start": 90.64, "end": 91.06, "word": " نسبة", "probability": 0.9869791666666666}, {"start": 91.06, "end": 91.6, "word": " الإصابة", "probability": 0.975830078125}, {"start": 91.6, "end": 91.88, "word": " كم", "probability": 0.675537109375}, {"start": 91.88, "end": 92.18, "word": " مرة", "probability": 0.9814453125}, {"start": 92.18, "end": 92.68, "word": " بيوصب", "probability": 0.78388671875}, {"start": 92.68, "end": 92.94, "word": " البشر", "probability": 0.82568359375}, {"start": 92.94, "end": 93.44, "word": " بهذا", "probability": 0.736083984375}, {"start": 93.44, "end": 93.92, "word": " ال", "probability": 0.7900390625}, {"start": 93.92, "end": 94.22, "word": " micro", "probability": 0.82763671875}, {"start": 94.22, "end": 94.82, "word": " لأنه", "probability": 0.52001953125}, {"start": 94.82, "end": 95.42, "word": " هتكلم", "probability": 0.935302734375}, {"start": 95.42, "end": 95.6, "word": " عن", "probability": 0.873046875}, {"start": 95.6, "end": 95.7, "word": " ال", "probability": 0.927734375}, {"start": 95.7, "end": 96.56, "word": " Staphylococci", "probability": 0.8232014973958334}, {"start": 96.56, "end": 97.32, "word": " برضه", "probability": 0.9874674479166666}, {"start": 97.32, "end": 98.26, "word": " بأكتر", "probability": 0.860009765625}, {"start": 98.26, "end": 98.94, "word": " لكن", "probability": 0.91455078125}, {"start": 98.94, "end": 99.4, "word": " أكتر", "probability": 0.9671630859375}, {"start": 99.4, "end": 99.58, "word": " من", "probability": 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بعتها in clusters واتفقنا أنه لا نستطيع حكم على ال clusters إلا من خلال اللي هو ال catalase test إذا كانت catalase بوستف هيستفيله كوكايل it is catalase بوستف major component of the nose and skin normal microbiota شوفنا أشكال إلها من ال pure culture و أشكال من عينات صديق", "tokens": [2304, 1829, 11622, 9307, 11296, 25961, 2655, 8717, 7435, 2288, 7649, 4724, 2407, 14851, 5172, 6156, 19452, 2655, 6055, 3555, 4587, 7578, 8717, 4587, 1211, 3224, 2423, 17620, 4724, 34268, 11296, 294, 23313, 4032, 9307, 5172, 4587, 8315, 14739, 3224, 20193, 8717, 14851, 9566, 40228, 11331, 24793, 15844, 2423, 23313, 11933, 15040, 9154, 16490, 1211, 6027, 13672, 1829, 31439, 2423, 13192, 651, 1500, 11933, 15730, 25961, 2655, 13192, 651, 4724, 2407, 14851, 5172, 39896, 14851, 5172, 26895, 3224, 9122, 2407, 4117, 995, 26895, 309, 307, 13192, 651, 4724, 2407, 14851, 5172, 2563, 6542, 295, 264, 6690, 293, 3178, 2710, 33234, 5377, 13412, 38688, 8315, 5551, 8592, 4117, 6027, 11933, 1211, 11296, 9154, 2423, 6075, 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486.47, "end": 515.53, "text": "الـ Staphylococcus aureus من الميكروبات المشهورة مشهورة المعنى آخر يعني موجودة في مصاف النجوم في عالم الـ Antimicrobial resistance من أوائل الميكروبات أربعينية القرن الماضي البنسالين تم اكتشافه كان هو ال magic لكل magic drug لكل Staphylococcal infection بعد خمساشر سنة بدأنا نلاحظ resistance طورنا ال penicillin وفي الآخر", "tokens": [6027, 39184, 745, 569, 3495, 5842, 905, 1149, 257, 540, 301, 9154, 9673, 1829, 4117, 32887, 3555, 9307, 9673, 8592, 3224, 13063, 3660, 37893, 3224, 13063, 3660, 9673, 3615, 1863, 7578, 19753, 34740, 37495, 22653, 3714, 29245, 23328, 3660, 8978, 3714, 9381, 31845, 28239, 7435, 20498, 8978, 6225, 45340, 2423, 39184, 5130, 332, 299, 16614, 831, 7335, 9154, 34051, 16373, 1211, 9673, 1829, 4117, 32887, 3555, 9307, 5551, 25513, 3615, 9957, 10632, 25062, 2288, 1863, 9673, 46958, 1829, 29739, 1863, 3794, 6027, 9957, 46811, 1975, 4117, 2655, 8592, 31845, 3224, 25961, 31439, 2423, 5585, 5296, 28820, 5585, 4110, 5296, 28820, 745, 569, 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0.8499755859375}, {"start": 491.33, "end": 491.65, "word": " آخر", "probability": 0.7283935546875}, {"start": 491.65, "end": 491.97, "word": " يعني", "probability": 0.930419921875}, {"start": 491.97, "end": 492.55, "word": " موجودة", "probability": 0.97314453125}, {"start": 492.55, "end": 492.69, "word": " في", "probability": 0.96337890625}, {"start": 492.69, "end": 493.25, "word": " مصاف", "probability": 0.7692057291666666}, {"start": 493.25, "end": 495.11, "word": " النجوم", "probability": 0.9627278645833334}, {"start": 495.11, "end": 495.55, "word": " في", "probability": 0.93896484375}, {"start": 495.55, "end": 495.97, "word": " عالم", "probability": 0.978271484375}, {"start": 495.97, "end": 496.31, "word": " الـ", "probability": 0.772216796875}, {"start": 496.31, "end": 497.01, "word": " Antimicrobial", "probability": 0.878662109375}, {"start": 497.01, "end": 497.61, "word": " resistance", "probability": 0.572265625}, {"start": 497.61, "end": 498.09, "word": " من", "probability": 0.94921875}, {"start": 498.09, "end": 498.41, "word": " أوائل", "probability": 0.8326822916666666}, {"start": 498.41, "end": 499.15, "word": " الميكروبات", "probability": 0.9612630208333334}, {"start": 499.15, "end": 500.19, "word": " أربعينية", "probability": 0.718701171875}, {"start": 500.19, "end": 500.53, "word": " القرن", "probability": 0.98779296875}, {"start": 500.53, "end": 501.21, "word": " الماضي", "probability": 0.9703776041666666}, {"start": 501.21, "end": 502.25, "word": " البنسالين", "probability": 0.739794921875}, {"start": 502.25, "end": 502.41, "word": " تم", "probability": 0.99755859375}, {"start": 502.41, "end": 502.99, "word": " اكتشافه", "probability": 0.908935546875}, {"start": 502.99, "end": 503.25, "word": " كان", "probability": 0.900390625}, {"start": 503.25, "end": 503.39, "word": " هو", "probability": 0.98681640625}, {"start": 503.39, "end": 503.55, "word": " ال", "probability": 0.8974609375}, {"start": 503.55, "end": 503.93, "word": " magic", "probability": 0.92431640625}, {"start": 503.93, "end": 504.77, "word": " لكل", "probability": 0.9521484375}, {"start": 504.77, "end": 505.25, "word": " magic", "probability": 0.9189453125}, {"start": 505.25, "end": 505.69, "word": " drug", "probability": 0.9052734375}, {"start": 505.69, "end": 506.47, "word": " لكل", "probability": 0.959716796875}, {"start": 506.47, "end": 507.43, "word": " Staphylococcal", "probability": 0.9042154947916666}, {"start": 507.43, "end": 508.07, "word": " infection", "probability": 0.8935546875}, {"start": 508.07, "end": 508.81, "word": " بعد", "probability": 0.93603515625}, {"start": 508.81, "end": 509.37, "word": " خمساشر", "probability": 0.86337890625}, {"start": 509.37, "end": 509.67, "word": " سنة", "probability": 0.9931640625}, {"start": 509.67, "end": 510.05, "word": " بدأنا", "probability": 0.9140625}, {"start": 510.05, "end": 510.55, "word": " نلاحظ", "probability": 0.9921875}, {"start": 510.55, "end": 511.31, "word": " resistance", "probability": 0.81201171875}, {"start": 511.31, "end": 512.19, "word": " طورنا", "probability": 0.8951822916666666}, {"start": 512.19, "end": 512.57, "word": " ال", "probability": 0.962890625}, {"start": 512.57, "end": 513.21, "word": " penicillin", "probability": 0.805419921875}, {"start": 513.21, "end": 515.03, "word": " وفي", "probability": 0.6971435546875}, {"start": 515.03, "end": 515.53, "word": " الآخر", "probability": 0.966796875}], "temperature": 1.0}, {"id": 22, "seek": 53767, "start": 516.12, "end": 537.68, "text": "حصلنا على وضع مذير للغاية شايفين الأسماء الأخيرة اللي موجودة على اللوحة اللي هي penicillin, meticillin و vancobicin اللي هون هي penicillin resistant بنسبة 95% ل 99% في غزة عندنا هنا بنسبة من 95% ل 99% ال meticillin وصلت ال resistance rate بعتها لأكتر من 40%", "tokens": [5016, 36520, 8315, 15844, 4032, 11242, 3615, 3714, 8848, 13546, 24976, 17082, 995, 10632, 13412, 995, 33911, 9957, 16247, 38251, 16606, 16247, 9778, 48923, 13672, 1829, 3714, 29245, 23328, 3660, 15844, 13672, 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518.96, "end": 519.82, "word": " شايفين", "probability": 0.8074951171875}, {"start": 519.82, "end": 520.28, "word": " الأسماء", "probability": 0.9698893229166666}, {"start": 520.28, "end": 520.66, "word": " الأخيرة", "probability": 0.9619140625}, {"start": 520.66, "end": 520.8, "word": " اللي", "probability": 0.5968017578125}, {"start": 520.8, "end": 520.98, "word": " موجودة", "probability": 0.76763916015625}, {"start": 520.98, "end": 521.06, "word": " على", "probability": 0.91015625}, {"start": 521.06, "end": 521.52, "word": " اللوحة", "probability": 0.976806640625}, {"start": 521.52, "end": 522.1, "word": " اللي", "probability": 0.677001953125}, {"start": 522.1, "end": 522.38, "word": " هي", "probability": 0.70751953125}, {"start": 522.38, "end": 523.14, "word": " penicillin,", "probability": 0.8677978515625}, {"start": 523.34, "end": 524.02, "word": " meticillin", "probability": 0.8095703125}, {"start": 524.02, "end": 524.26, "word": " و", "probability": 0.87744140625}, {"start": 524.26, "end": 525.06, "word": " vancobicin", "probability": 0.68662109375}, {"start": 525.06, "end": 525.8, "word": " اللي", "probability": 0.797119140625}, {"start": 525.8, "end": 525.88, "word": " هون", "probability": 0.427978515625}, {"start": 525.88, "end": 526.0, "word": " هي", "probability": 0.40966796875}, {"start": 526.0, "end": 526.5, "word": " penicillin", "probability": 0.9415283203125}, {"start": 526.5, "end": 526.84, "word": " resistant", "probability": 0.90673828125}, {"start": 526.84, "end": 527.38, "word": " بنسبة", "probability": 0.8878580729166666}, {"start": 527.38, "end": 528.1, "word": " 95", "probability": 0.943359375}, {"start": 528.1, "end": 528.34, "word": "%", "probability": 0.40771484375}, {"start": 528.34, "end": 528.36, "word": " ل", "probability": 0.58642578125}, {"start": 528.36, "end": 528.64, "word": " 99", "probability": 0.50048828125}, {"start": 528.64, "end": 529.12, "word": "%", "probability": 0.94189453125}, {"start": 529.12, "end": 530.14, "word": " في", "probability": 0.63232421875}, {"start": 530.14, "end": 530.38, "word": " غزة", "probability": 0.7753092447916666}, {"start": 530.38, "end": 530.66, "word": " عندنا", "probability": 0.6624755859375}, {"start": 530.66, "end": 530.86, "word": " هنا", "probability": 0.7421875}, {"start": 530.86, "end": 531.44, "word": " بنسبة", "probability": 0.7884928385416666}, {"start": 531.44, "end": 531.6, "word": " من", "probability": 0.896484375}, {"start": 531.6, "end": 532.06, "word": " 95", "probability": 0.98291015625}, {"start": 532.06, "end": 532.32, "word": "%", "probability": 0.90234375}, {"start": 532.32, "end": 532.6, "word": " ل", "probability": 0.58544921875}, {"start": 532.6, "end": 532.98, "word": " 99", "probability": 0.9560546875}, {"start": 532.98, "end": 533.48, "word": "%", "probability": 0.99072265625}, {"start": 533.48, "end": 534.66, "word": " ال", "probability": 0.33349609375}, {"start": 534.66, "end": 535.18, "word": " meticillin", "probability": 0.87744140625}, {"start": 535.18, "end": 535.6, "word": " وصلت", "probability": 0.8211263020833334}, {"start": 535.6, "end": 535.7, "word": " ال", "probability": 0.9345703125}, {"start": 535.7, "end": 536.12, "word": " resistance", "probability": 0.9765625}, {"start": 536.12, "end": 536.42, "word": " rate", "probability": 0.5986328125}, {"start": 536.42, "end": 536.8, "word": " بعتها", "probability": 0.6226399739583334}, {"start": 536.8, "end": 537.06, "word": " لأكتر", "probability": 0.92802734375}, {"start": 537.06, "end": 537.16, "word": " من", "probability": 0.99169921875}, {"start": 537.16, "end": 537.38, "word": " 40", "probability": 0.9599609375}, {"start": 537.38, "end": 537.68, "word": "%", "probability": 0.990234375}], "temperature": 1.0}, {"id": 23, "seek": 56778, "start": 542.68, "end": 567.78, "text": "بواد الظهور ل Vancomycin-resistant Staphylococcus aureus دراسة قبل أقل من شهر طلعت تلاتة و نص فالمية يعني معناه الحاجة إن أنا فيها عندي مشكلة حقيقية يعني هايجي يوم من الأيام لأنا استطيع أن أعالج Staphylococcus aureus بال available drugs اللي موجودة في الأسواق طبعا ال available drugs اللي هي يمكن استخدامها استخدام للأدم", "tokens": [3555, 14407, 3215, 6024, 116, 3224, 13063, 5296, 8979, 1112, 88, 20021, 12, 495, 10329, 745, 569, 3495, 5842, 905, 1149, 257, 540, 301, 11778, 23557, 3794, 3660, 12174, 36150, 5551, 4587, 1211, 9154, 13412, 3224, 2288, 23032, 1211, 34268, 6055, 1211, 9307, 3660, 4032, 8717, 9381, 6156, 45340, 10632, 37495, 22653, 20449, 8315, 3224, 21542, 26108, 3660, 36145, 41850, 8978, 11296, 18871, 16254, 37893, 28820, 3660, 11331, 38436, 4587, 10632, 37495, 22653, 8032, 47302, 7435, 1829, 7251, 20498, 9154, 16247, 1829, 10943, 5296, 10721, 8315, 44713, 9566, 40228, 14739, 5551, 3615, 6027, 7435, 745, 569, 3495, 5842, 905, 1149, 257, 540, 301, 20666, 2435, 7766, 13672, 1829, 3714, 29245, 23328, 3660, 8978, 16247, 3794, 14407, 4587, 23032, 3555, 3615, 995, 2423, 2435, 7766, 13672, 1829, 39896, 7251, 43020, 44713, 9778, 3215, 10943, 11296, 44713, 9778, 3215, 10943, 24976, 10721, 40448], "avg_logprob": -0.23591549935894954, "compression_ratio": 1.7543859649122806, "no_speech_prob": 0.0, "words": [{"start": 542.6800000000001, "end": 543.46, "word": "بواد", "probability": 0.8986002604166666}, {"start": 543.46, "end": 544.1, "word": " الظهور", "probability": 0.831787109375}, {"start": 544.1, "end": 544.6, "word": " ل", "probability": 0.90673828125}, {"start": 544.6, "end": 545.5, "word": " Vancomycin", "probability": 0.76953125}, {"start": 545.5, "end": 545.96, "word": "-resistant", "probability": 0.7360026041666666}, {"start": 545.96, "end": 546.68, "word": " Staphylococcus", "probability": 0.8521321614583334}, {"start": 546.68, "end": 547.0, "word": " aureus", "probability": 0.83349609375}, {"start": 547.0, "end": 547.68, "word": " دراسة", "probability": 0.920654296875}, {"start": 547.68, "end": 548.06, "word": " قبل", "probability": 0.978759765625}, {"start": 548.06, "end": 549.1, "word": " أقل", "probability": 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554.62, "end": 554.86, "word": " فيها", "probability": 0.873291015625}, {"start": 554.86, "end": 555.08, "word": " عندي", "probability": 0.6298828125}, {"start": 555.08, "end": 555.62, "word": " مشكلة", "probability": 0.9869791666666666}, {"start": 555.62, "end": 556.08, "word": " حقيقية", "probability": 0.9937744140625}, {"start": 556.08, "end": 556.24, "word": " يعني", "probability": 0.827392578125}, {"start": 556.24, "end": 556.54, "word": " هايجي", "probability": 0.67120361328125}, {"start": 556.54, "end": 556.72, "word": " يوم", "probability": 0.994384765625}, {"start": 556.72, "end": 556.8, "word": " من", "probability": 0.9609375}, {"start": 556.8, "end": 557.22, "word": " الأيام", "probability": 0.9811197916666666}, {"start": 557.22, "end": 557.98, "word": " لأنا", "probability": 0.4988606770833333}, {"start": 557.98, "end": 558.34, "word": " استطيع", "probability": 0.8592122395833334}, {"start": 558.34, "end": 558.48, "word": " أن", "probability": 0.556640625}, {"start": 558.48, "end": 558.88, "word": " أعالج", "probability": 0.8026123046875}, {"start": 558.88, "end": 559.54, "word": " Staphylococcus", "probability": 0.9176432291666666}, {"start": 559.54, "end": 559.8, "word": " aureus", "probability": 0.9103190104166666}, {"start": 559.8, "end": 559.94, "word": " بال", "probability": 0.9384765625}, {"start": 559.94, "end": 560.34, "word": " available", "probability": 0.39697265625}, {"start": 560.34, "end": 560.76, "word": " drugs", "probability": 0.88525390625}, {"start": 560.76, "end": 561.04, "word": " اللي", "probability": 0.890380859375}, {"start": 561.04, "end": 561.84, "word": " موجودة", "probability": 0.9912109375}, {"start": 561.84, "end": 562.18, "word": " في", "probability": 0.95361328125}, {"start": 562.18, "end": 562.92, "word": " الأسواق", "probability": 0.984130859375}, {"start": 562.92, "end": 563.3, "word": " طبعا", "probability": 0.9522705078125}, {"start": 563.3, "end": 563.42, "word": " ال", "probability": 0.92333984375}, {"start": 563.42, "end": 563.7, "word": " available", "probability": 0.8212890625}, {"start": 563.7, "end": 564.06, "word": " drugs", "probability": 0.89404296875}, {"start": 564.06, "end": 564.2, "word": " اللي", "probability": 0.96044921875}, {"start": 564.2, "end": 564.38, "word": " هي", "probability": 0.8623046875}, {"start": 564.38, "end": 565.6, "word": " يمكن", "probability": 0.859375}, {"start": 565.6, "end": 566.26, "word": " استخدامها", "probability": 0.97421875}, {"start": 566.26, "end": 567.0, "word": " استخدام", "probability": 0.9599609375}, {"start": 567.0, "end": 567.78, "word": " للأدم", "probability": 0.798828125}], "temperature": 1.0}, {"id": 24, "seek": 59466, "start": 569.68, "end": 594.66, "text": "الصورة اللي قدامنا هي دي بتوضح ال hemolysis اللي حكينا عنه في المحاضرة السابقة اللي هو beta .. beta hemolysis complete hemolysis .. complete hemolysis الصورة هي بتوضح ان ال .. ال .. مش ال hemolysis تعفو عنه ال manitol salt agar هذا بده وقفة ويردت نتركز عنه شوية ال manitol salt agar هو عبارة عن selective and differential كلكوا بتعرف إيش أنا selective و إيش أنا differential", "tokens": [6027, 9381, 13063, 3660, 13672, 1829, 12174, 3215, 10943, 8315, 39896, 11778, 1829, 39894, 2407, 11242, 5016, 2423, 8636, 16453, 17122, 13672, 1829, 11331, 4117, 1829, 8315, 18871, 3224, 8978, 9673, 5016, 46958, 25720, 21136, 16758, 28671, 13672, 1829, 31439, 9861, 4386, 9861, 8636, 16453, 17122, 3566, 8636, 16453, 17122, 4386, 3566, 8636, 16453, 17122, 31767, 13063, 3660, 39896, 39894, 2407, 11242, 5016, 16472, 2423, 4386, 2423, 4386, 37893, 2423, 8636, 16453, 17122, 37279, 5172, 2407, 18871, 3224, 2423, 587, 21086, 5139, 623, 289, 23758, 47525, 3224, 4032, 4587, 5172, 3660, 4032, 13546, 3215, 2655, 8717, 2655, 31747, 11622, 18871, 3224, 13412, 2407, 10632, 2423, 587, 21086, 5139, 623, 289, 31439, 6225, 3555, 9640, 3660, 18871, 33930, 293, 15756, 28242, 4117, 14407, 39894, 3615, 28480, 11933, 1829, 8592, 41850, 33930, 4032, 11933, 1829, 8592, 41850, 15756], "avg_logprob": 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"probability": 0.892578125}, {"start": 581.5, "end": 581.76, "word": " تعفو", "probability": 0.4619954427083333}, {"start": 581.76, "end": 581.9, "word": " عنه", "probability": 0.5211181640625}, {"start": 581.9, "end": 582.0, "word": " ال", "probability": 0.669921875}, {"start": 582.0, "end": 582.28, "word": " manitol", "probability": 0.25872802734375}, {"start": 582.28, "end": 582.58, "word": " salt", "probability": 0.72119140625}, {"start": 582.58, "end": 582.86, "word": " agar", "probability": 0.684326171875}, {"start": 582.86, "end": 583.62, "word": " هذا", "probability": 0.900390625}, {"start": 583.62, "end": 583.88, "word": " بده", "probability": 0.583740234375}, {"start": 583.88, "end": 584.26, "word": " وقفة", "probability": 0.9293212890625}, {"start": 584.26, "end": 584.64, "word": " ويردت", "probability": 0.489532470703125}, {"start": 584.64, "end": 585.1, "word": " نتركز", "probability": 0.7564697265625}, {"start": 585.1, "end": 585.3, "word": " عنه", "probability": 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لجوكاس", "probability": 0.4189453125}, {"start": 972.48, "end": 972.78, "word": " اوريس", "probability": 0.76934814453125}, {"start": 972.78, "end": 973.06, "word": " او", "probability": 0.7266845703125}, {"start": 973.06, "end": 973.84, "word": " لبعض", "probability": 0.9705810546875}, {"start": 973.84, "end": 974.06, "word": " انواع", "probability": 0.83154296875}, {"start": 974.06, "end": 974.22, "word": " من", "probability": 0.88671875}, {"start": 974.22, "end": 974.9, "word": " البكتيريا", "probability": 0.9482421875}, {"start": 974.9, "end": 975.32, "word": " اللى", "probability": 0.98193359375}, {"start": 975.32, "end": 976.02, "word": " بتستخدم", "probability": 0.9560546875}, {"start": 976.02, "end": 976.68, "word": " مركب", "probability": 0.7757975260416666}], "temperature": 1.0}, {"id": 40, "seek": 101094, "start": 981.62, "end": 1010.94, "text": "البوتاسيوم تليوريد مُركّب بصير له reduction من بعض البكتيريا reduction يعني utilization يعني استخدام مش كل البكتيريا تقدر تعمله reduction few bacteria can produce this substance أو use this substance منهم من الـ Staphylococcus aureus لما بتعمله reduction ويكون في الوسط هو ويكون colorless بصير لونه أسود فالمستعمرات بصير لونها أسود فبالتالي برضه بميز مين؟", "tokens": [6027, 3555, 35473, 32277, 1829, 20498, 6055, 20292, 13063, 25708, 3714, 10859, 31747, 11703, 3555, 4724, 9381, 13546, 46740, 11004, 9154, 45030, 11242, 29739, 4117, 2655, 13546, 25528, 11004, 37495, 22653, 37074, 37495, 22653, 44713, 9778, 3215, 10943, 37893, 28242, 29739, 4117, 2655, 13546, 25528, 6055, 28543, 2288, 6055, 25957, 1211, 3224, 11004, 1326, 11763, 393, 5258, 341, 12961, 34051, 764, 341, 12961, 9154, 16095, 9154, 2423, 39184, 745, 569, 3495, 5842, 905, 1149, 257, 540, 301, 5296, 15042, 39894, 25957, 43761, 11004, 4032, 1829, 30544, 8978, 2423, 41779, 9566, 31439, 4032, 1829, 30544, 2017, 1832, 4724, 9381, 13546, 5296, 11536, 3224, 5551, 3794, 23328, 6156, 45340, 14851, 25957, 2288, 9307, 4724, 9381, 13546, 5296, 11536, 11296, 5551, 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"probability": 0.967529296875}, {"start": 2859.55, "end": 2860.15, "word": " Non", "probability": 0.32958984375}, {"start": 2860.15, "end": 2860.83, "word": "-Growable", "probability": 0.84765625}, {"start": 2860.83, "end": 2861.89, "word": " من", "probability": 0.9423828125}, {"start": 2861.89, "end": 2862.09, "word": " أهم", "probability": 0.97802734375}, {"start": 2862.09, "end": 2862.83, "word": " المجموعات", "probability": 0.959765625}, {"start": 2862.83, "end": 2863.71, "word": " A,", "probability": 0.841796875}, {"start": 2863.87, "end": 2864.15, "word": " B", "probability": 0.55908203125}, {"start": 2864.15, "end": 2864.47, "word": " و", "probability": 0.71923828125}, {"start": 2864.47, "end": 2864.71, "word": " D", "probability": 0.58447265625}, {"start": 2864.71, "end": 2865.37, "word": " الـ", "probability": 0.612060546875}, {"start": 2865.37, "end": 2865.51, "word": " C", "probability": 0.6201171875}, {"start": 2865.51, "end": 2865.65, "word": " و", "probability": 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2871.47, "end": 2871.81, "word": " جاية", "probability": 0.9275716145833334}, {"start": 2871.81, "end": 2872.19, "word": " و", "probability": 0.229248046875}, {"start": 2872.19, "end": 2872.23, "word": " هي", "probability": 0.83984375}, {"start": 2872.23, "end": 2872.37, "word": " بس", "probability": 0.983642578125}, {"start": 2872.37, "end": 2872.59, "word": " بعد", "probability": 0.65966796875}, {"start": 2872.59, "end": 2872.87, "word": " السؤال", "probability": 0.8064778645833334}, {"start": 2872.87, "end": 2873.49, "word": " الأخرى", "probability": 0.6953125}, {"start": 2873.49, "end": 2873.61, "word": " في", "probability": 0.79296875}, {"start": 2873.61, "end": 2874.21, "word": " محاضرتنا", "probability": 0.97705078125}, {"start": 2874.21, "end": 2874.87, "word": " الـ", "probability": 0.835693359375}, {"start": 2874.87, "end": 2874.99, "word": " Non", "probability": 0.880859375}, {"start": 2874.99, "end": 2875.43, "word": "-Growable", "probability": 0.915771484375}, {"start": 2875.43, "end": 2875.87, "word": " تشمل", "probability": 0.9777018229166666}, {"start": 2875.87, "end": 2876.21, "word": " واحد", "probability": 0.992919921875}, {"start": 2876.21, "end": 2876.37, "word": " من", "probability": 0.98681640625}, {"start": 2876.37, "end": 2876.85, "word": " الميكروبات", "probability": 0.9296061197916666}, {"start": 2876.85, "end": 2877.07, "word": " المهم", "probability": 0.9892578125}, {"start": 2877.07, "end": 2877.45, "word": " جدا", "probability": 0.965087890625}, {"start": 2877.45, "end": 2877.91, "word": " اللي", "probability": 0.753662109375}, {"start": 2877.91, "end": 2878.01, "word": " هي", "probability": 0.464111328125}, {"start": 2878.01, "end": 2878.83, "word": " Steptococcus", "probability": 0.70361328125}, {"start": 2878.83, "end": 2879.55, "word": " pneumonia", "probability": 0.86474609375}], "temperature": 1.0}, {"id": 114, "seek": 289297, "start": 2879.99, "end": 2892.97, "text": "و إلها شبيه مورود في الفيلم بشكل كبير اسمه Stratococcus Mutans و بتسبب Dental Chaos او تسوص اللي هو الاسم باكن كل حاجة انهينا محاضرتنا و ان شاء الله نكمل مرة القاهرة", "tokens": [2407, 11933, 1211, 11296, 13412, 21292, 3224, 3714, 13063, 23328, 8978, 27188, 1829, 19528, 4724, 8592, 28820, 9122, 3555, 13546, 24525, 2304, 3224, 745, 4481, 905, 905, 1149, 18517, 599, 4032, 39894, 35457, 3555, 413, 14533, 32644, 1975, 2407, 6055, 3794, 2407, 9381, 13672, 1829, 31439, 2423, 32277, 2304, 4724, 995, 19452, 28242, 11331, 26108, 3660, 16472, 3224, 1829, 8315, 3714, 5016, 46958, 43500, 8315, 4032, 16472, 13412, 16606, 21984, 8717, 24793, 1211, 3714, 25720, 25062, 40294, 25720], "avg_logprob": -0.3164557067653801, "compression_ratio": 1.4438502673796791, "no_speech_prob": 0.0, "words": [{"start": 2879.99, "end": 2880.27, "word": "و", "probability": 0.97509765625}, {"start": 2880.27, "end": 2880.59, "word": " إلها", "probability": 0.6217447916666666}, {"start": 2880.59, "end": 2881.41, "word": " شبيه", "probability": 0.89697265625}, {"start": 2881.41, 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0.2861328125}, {"start": 2887.03, "end": 2887.21, "word": " او", "probability": 0.6529541015625}, {"start": 2887.21, "end": 2888.43, "word": " تسوص", "probability": 0.67974853515625}, {"start": 2888.43, "end": 2888.59, "word": " اللي", "probability": 0.78466796875}, {"start": 2888.59, "end": 2888.79, "word": " هو", "probability": 0.98779296875}, {"start": 2888.79, "end": 2889.47, "word": " الاسم", "probability": 0.726806640625}, {"start": 2889.47, "end": 2890.37, "word": " باكن", "probability": 0.4414265950520833}, {"start": 2890.37, "end": 2890.53, "word": " كل", "probability": 0.64306640625}, {"start": 2890.53, "end": 2890.71, "word": " حاجة", "probability": 0.7254231770833334}, {"start": 2890.71, "end": 2890.95, "word": " انهينا", "probability": 0.6429443359375}, {"start": 2890.95, "end": 2891.43, "word": " محاضرتنا", "probability": 0.91015625}, {"start": 2891.43, "end": 2891.55, "word": " و", "probability": 0.98876953125}, {"start": 2891.55, "end": 2891.57, "word": " ان", "probability": 0.787109375}, {"start": 2891.57, "end": 2891.71, "word": " شاء", "probability": 0.95849609375}, {"start": 2891.71, "end": 2891.73, "word": " الله", "probability": 0.96142578125}, {"start": 2891.73, "end": 2892.05, "word": " نكمل", "probability": 0.9886067708333334}, {"start": 2892.05, "end": 2892.31, "word": " مرة", "probability": 0.77734375}, {"start": 2892.31, "end": 2892.97, "word": " القاهرة", "probability": 0.7137858072916666}], "temperature": 1.0}], "language": "ar", "language_probability": 1.0, "duration": 2894.50675, "duration_after_vad": 2699.91187499999} \ No newline at end of file diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/uz47-1oLtNU_raw.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/uz47-1oLtNU_raw.srt new file mode 100644 index 0000000000000000000000000000000000000000..23d22a0ee81a8b1932f43da14649ad6cb37df724 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/uz47-1oLtNU_raw.srt @@ -0,0 +1,2572 @@ +1 +00:00:20,860 --> 00:00:25,120 +بسم الله الرحمن الرحيم نكمل مع بعض سويا الموضوع + +2 +00:00:25,120 --> 00:00:29,840 +بعنوان biogenic coccus وكلمة biogenic coccus + +3 +00:00:29,840 --> 00:00:33,480 +اتفقنا على انه بيربطها معنايا اساسيا ان البكتيريا + +4 +00:00:33,480 --> 00:00:37,780 +اللي بتتحدث عنها هي كوكساي كراويت الشكل ومن سماتها + +5 +00:00:37,780 --> 00:00:40,980 +انها بتعمل biogenic infection او ما يسمى بال pass + +6 +00:00:40,980 --> 00:00:46,830 +producing infectionاطلعنا على بعض الصور اللى بتوضح + +7 +00:00:46,830 --> 00:00:52,690 +الأشكال المختلفة اللى هندرسها تحت هذا الموضوع شفنا + +8 +00:00:52,690 --> 00:00:59,370 +شكلها رئيسيين اللى هم ال chains و ال clusters ال + +9 +00:00:59,370 --> 00:01:03,450 +chains و ال clusters في اللوحات اللى أمامكوا + +10 +00:01:03,450 --> 00:01:07,670 +معظمهم ل ال strip to koukai فقط الوحيدة اللى منهم + +11 +00:01:07,670 --> 00:01:12,830 +استفى ل koukai اللى على يمين الشاشة في الأعلى + +12 +00:01:18,420 --> 00:01:21,240 +وبدأنا بالحديثة عن الـ Staphylococci والـ + +13 +00:01:21,240 --> 00:01:24,940 +Staphylococci هي المجموع الأهم حقيقة من حيث عدد ال + +14 +00:01:24,940 --> 00:01:28,520 +infection دي اللي بتسببها كلمة عدد يعني بتتكلم + +15 +00:01:28,520 --> 00:01:32,180 +فيها frequency frequency يعني نسبة الإصابة كم مرة + +16 +00:01:32,180 --> 00:01:35,700 +بيوصب البشر بهذا ال micro لأنه هتكلم عن ال + +17 +00:01:35,700 --> 00:01:40,860 +Staphylococci برضه بأكتر لكن أكتر من ناحية الأنواع + +18 +00:01:40,860 --> 00:01:44,620 +وليس ال frequency أنواع ال infection ال + +19 +00:01:44,620 --> 00:01:49,680 +Staphylococciميزاتها كانت نجران بوستف فكنت تبقى + +20 +00:01:49,680 --> 00:01:53,340 +نقله ال arrangement بعتها in clusters واتفقنا أنه + +21 +00:01:53,340 --> 00:01:57,320 +لا نستطيع حكم على ال clusters إلا من خلال اللي هو + +22 +00:01:57,320 --> 00:02:00,720 +ال catalase test إذا كانت catalase بوستف هيستفيله + +23 +00:02:00,720 --> 00:02:04,560 +كوكايل it is catalase بوستف major component of the + +24 +00:02:04,560 --> 00:02:10,660 +nose and skin normal microbiota شوفنا أشكال إلها + +25 +00:02:10,660 --> 00:02:16,540 +من ال pure culture و أشكال من عينات صديقشفنا بحث + +26 +00:02:16,540 --> 00:02:19,320 +ال catalase اللى تحدثنا عنه المرة الماضية و قلنا + +27 +00:02:19,320 --> 00:02:21,480 +انه يبقى عن قطرة من hydrogen peroxide و ضعف إلى + +28 +00:02:21,480 --> 00:02:24,360 +البكتيريا إذا طلع عندنا ال bubbles فهي agostin إذا + +29 +00:02:24,360 --> 00:02:28,240 +ما طلع عندنا ال bubbles بتكون catalaseوبدأنا + +30 +00:02:28,240 --> 00:02:30,380 +بالحديث اللي على الوضع الأصعب اللي هو ال + +31 +00:02:30,380 --> 00:02:32,420 +pathogenesis و ال immunity للـ Staphylococcus و + +32 +00:02:32,420 --> 00:02:35,860 +اتكلمنا عن اللي هو ال capsule، اتكلمنا عن ال + +33 +00:02:35,860 --> 00:02:39,440 +protein A، اتكلمنا عن ال liquid glycan، اتكلمنا عن + +34 +00:02:39,440 --> 00:02:44,300 +ال clumping factor أو ال coagulase substance اللي + +35 +00:02:44,300 --> 00:02:48,920 +موجود في البكتيريا، وشوفنا صورة لل protein A كيف + +36 +00:02:48,920 --> 00:02:52,300 +بيجلب أو بيرتبط بال FC portion دبعه اللي هو ال + +37 +00:02:52,300 --> 00:02:56,430 +antibioticمجموعة من الـ enzymes والتوكسين برضه + +38 +00:02:56,430 --> 00:02:59,630 +لوحة كاملة وركزنا في آخر الرزقين اللي هم الـtoxic + +39 +00:02:59,630 --> 00:03:03,050 +shock syndrome toxin والـ enterotoxin واتفقنا على + +40 +00:03:03,050 --> 00:03:06,010 +أن الاتنين super antigens لكن واحد acted + +41 +00:03:06,010 --> 00:03:08,670 +systematically وواحد acted locally في ال + +42 +00:03:08,670 --> 00:03:13,830 +intestinal tract وهذا الشكل أو الصورة وضحت لنا ال + +43 +00:03:13,830 --> 00:03:16,830 +mechanism by which super antigen works by joining + +44 +00:03:16,830 --> 00:03:21,730 +two cells اللي هم ال T cell and اللي قلنا عنها ال + +45 +00:03:21,730 --> 00:03:27,710 +macrofageو برضه تعرجنا على بعض الأمراض اللي اللي + +46 +00:03:27,710 --> 00:03:30,070 +علاقة بال skin و ال systemic و قولنا إنه في ال + +47 +00:03:30,070 --> 00:03:33,190 +skin فيه pharyncles و فينا style اللي قولنا .. + +48 +00:03:33,190 --> 00:03:36,830 +اللي سميناها بالعربي شحات ال N-P-T-G و الحصف ال + +49 +00:03:36,830 --> 00:03:40,050 +Imidermal Necrolysis و ال exfoliative dermatitis و + +50 +00:03:40,050 --> 00:03:44,230 +تانية قولنا فيهم دتلة الخلايا تبعات الجلب و ال + +51 +00:03:44,230 --> 00:03:47,470 +mastitis اللي هي تهيأة التدري أو الضرر abscess + +52 +00:03:47,470 --> 00:03:50,970 +اللي قولنا عنها هي deep pharyncles تمام؟ بتكون + +53 +00:03:50,970 --> 00:03:57,440 +مولودة في .. إنه في ال ..الكبد او في الدماغ او في + +54 +00:03:57,440 --> 00:04:02,920 +اماكن لكن اتفقنا على ان كل ال infections هذه ليها + +55 +00:04:02,920 --> 00:04:03,140 +ال + +56 +00:04:06,090 --> 00:04:09,810 +ال deep tissues بتكون الإصابة جاية من wound + +57 +00:04:09,810 --> 00:04:12,790 +infection على سبيل المثال يعني مثلا إلتهاب جروح أو + +58 +00:04:12,790 --> 00:04:18,170 +كأن abscess خارجي أو فرنكلز ومن ثم تخرب البكتيريا + +59 +00:04:18,170 --> 00:04:22,230 +من مكانها الأصلي إلى الدم و بتعمل اللي هي سبتسيميا + +60 +00:04:22,230 --> 00:04:27,650 +ممكن تصل إلى مكان مختلف ومتنوع خفنا الصورة هذه + +61 +00:04:27,650 --> 00:04:31,110 +برضه و اتكلمنا عن ان هذه ال impetigo وقولنا ان هذا + +62 +00:04:31,110 --> 00:04:34,230 +very contagious ويمكن الأول مرة نستخدم مصطلح + +63 +00:04:34,230 --> 00:04:38,750 +contagiousInfectious معناها معضي بينتقل من إنسان + +64 +00:04:38,750 --> 00:04:42,510 +إلى إنسان أو من حيوان إلى إنسان Contagious هو معضي + +65 +00:04:42,510 --> 00:04:45,670 +لكن بدرجة أكتر من كلمة infectious بمعنى أخر أن + +66 +00:04:45,670 --> 00:04:51,230 +نسبة العدوى فيه بتكون سريعة وعالية وبرضه كمان + +67 +00:04:51,230 --> 00:04:53,990 +اتطرقنا لموضوع مهم جدا لما اتكلمنا عن ال food + +68 +00:04:53,990 --> 00:04:57,370 +poisoning وميزنا بين ال food poisoning و ال food + +69 +00:04:57,370 --> 00:05:02,290 +infectionوأكد على أن ال food poisoning ارتبط + +70 +00:05:02,290 --> 00:05:09,110 +تسميته بتناول الإنسان بتناوله طعام يحتوي على تكسين + +71 +00:05:09,110 --> 00:05:12,370 +مش بالضرورة على الميكروب يمكن الميكروب يكون قام + +72 +00:05:12,370 --> 00:05:16,350 +بالتكسين ومات لكن أنا تناولت فقط أو ال symptoms + +73 +00:05:16,350 --> 00:05:19,610 +اللي صارت عندنا من وين؟ من التكسين بينما ال food + +74 +00:05:19,610 --> 00:05:23,250 +infection تناولنا الغذاء فيه micro organism قد + +75 +00:05:23,250 --> 00:05:26,230 +يكون العدد قليل لكن كافي أن يحدث عندي أنا + +76 +00:05:26,230 --> 00:05:30,230 +infectionو growth و بعدين الميكروب هو ينتج ال + +77 +00:05:30,230 --> 00:05:32,970 +toxin ويعمل disease في الداخل هذا سمينه food + +78 +00:05:32,970 --> 00:05:36,750 +infection فبنميز بين food poisoning و food + +79 +00:05:36,750 --> 00:05:40,910 +infection غالبا ال poisoning أعراضه بتبدأ سريعا + +80 +00:05:40,910 --> 00:05:44,170 +بينما ال infection قد تستمر يومين تلاتة أو تبدأ + +81 +00:05:44,170 --> 00:05:46,490 +بعد يومين أو تلاتة حسب نوع الميكروب اللي بيتكلم + +82 +00:05:46,490 --> 00:05:50,820 +عنههذه طبعا الصورة كانت لل toxic shock syndrome + +83 +00:05:50,820 --> 00:05:54,080 +لما اتكلمنا عن ال tampons وقلنا انه فيه مجموعة من + +84 +00:05:54,080 --> 00:05:56,940 +ال symptoms زي ال fever ال rashes و ال + +85 +00:05:56,940 --> 00:06:01,060 +disquamation و ال vomiting و ال diarrhea و ال + +86 +00:06:01,060 --> 00:06:04,120 +myelias و ان ال myelias اللي هي ألم العضلات + +87 +00:06:06,740 --> 00:06:09,560 +طبعا هذا الصورة ايضا اعتقد انها انهينا فيها + +88 +00:06:09,560 --> 00:06:12,260 +المحاضرة اللي اتكلمنا عنه اللي هو scalded skin + +89 +00:06:12,260 --> 00:06:16,600 +syndrome اللي جونا فيه توكسين اسمه X-folatein وهذا + +90 +00:06:16,600 --> 00:06:19,740 +ال X-folatein هو المسئول عن ال peeling off لل skin + +91 +00:06:19,740 --> 00:06:25,200 +تبع ال babies المصابينلا اعتقد ان احنا وصلنا ل .. + +92 +00:06:25,200 --> 00:06:29,060 +وصلنا لمرحلة و الصورة اللي قولنا عنها دي الصورة + +93 +00:06:29,060 --> 00:06:33,160 +اللي موجودة هي تعبيرة عن حالة واحدة اسمها Faj + +94 +00:06:33,160 --> 00:06:36,700 +typing يعني مش خاصة بالصفر ال Kukas ولكن معبرة عن + +95 +00:06:36,700 --> 00:06:40,120 +اي حالة اسمها Faj typing و الفراغات اللي احنا + +96 +00:06:40,120 --> 00:06:45,420 +شايفينها الصغيرة ها دي هي عبارة عن مناطق الفاج عمل + +97 +00:06:45,420 --> 00:06:48,660 +فيها infection و بالتالي صار فيها lysis سمناها + +98 +00:06:48,660 --> 00:06:55,310 +blacksأي Lysis من قبل الفاج يعني أن الفاج specific + +99 +00:06:55,310 --> 00:06:59,910 +للبكتيريا، إذا كان الفاج معروف والبكتيريا مجهولة + +100 +00:07:01,030 --> 00:07:05,310 +واتفقنا على انه يستخدم فيه عميق التعامل ثلاث صفات + +101 +00:07:05,310 --> 00:07:09,110 +للستفيلو كوكاس اوريس ذكرناهم المحاضرة السابقة بدنا + +102 +00:07:09,110 --> 00:07:13,150 +نأكد عليهم مرة أخرى manitol fermentation coagulase + +103 +00:07:13,150 --> 00:07:17,490 +test والديانيز ثلاث صفات positive تلاتة positive + +104 +00:07:17,490 --> 00:07:21,330 +بيميزوا الستفيلو كوكاس اوريس عن باقي أنواع + +105 +00:07:21,330 --> 00:07:25,750 +الستفيلو كوكا أحدهم بيسأل بيقول انه طيب بكفي ال + +106 +00:07:25,750 --> 00:07:29,290 +coagulase اللي خدته في المعمرهو الكوكاليش مميز من + +107 +00:07:29,290 --> 00:07:33,350 +الاستفيل الكوكا أول سباق الاستفيل الكوكا القاعش + +108 +00:07:33,350 --> 00:07:33,690 +بتقول + +109 +00:07:38,460 --> 00:07:41,380 +the more test you use the more accurate is your + +110 +00:07:41,380 --> 00:07:44,780 +diagnosis كل ما زاد عدد الفحوصات كل ما زاد دقة + +111 +00:07:44,780 --> 00:07:49,280 +التعرف لعل لو عشان نكون في microbe احنا مابنعرفه + +112 +00:07:49,280 --> 00:07:52,360 +أساسا يعني environmental microbe بيسمي ال microbe + +113 +00:07:52,360 --> 00:07:57,980 +بنعرفه وبالتالي لسنا بيصاد هنا انه نعمل تعريف غير + +114 +00:07:57,980 --> 00:08:02,980 +دقيق بدنا تعريف دقيق E ركزنا عليها ولا ماركزنا + +115 +00:08:02,980 --> 00:08:07,430 +عليها ال antibiotic sensitivityالـ Staphylococcus + +116 +00:08:07,430 --> 00:08:11,650 +aureus من الميكروبات المشهورة مشهورة المعنى آخر + +117 +00:08:11,650 --> 00:08:16,310 +يعني موجودة في مصاف النجوم في عالم الـ + +118 +00:08:16,310 --> 00:08:19,150 +Antimicrobial resistance من أوائل الميكروبات + +119 +00:08:19,150 --> 00:08:23,390 +أربعينية القرن الماضي البنسالين تم اكتشافه كان هو + +120 +00:08:23,390 --> 00:08:27,430 +ال magic لكل magic drug لكل Staphylococcal + +121 +00:08:27,430 --> 00:08:31,310 +infection بعد خمساشر سنة بدأنا نلاحظ resistance + +122 +00:08:31,310 --> 00:08:38,380 +طورنا ال penicillin وفي الآخرحصلنا على وضع مذير + +123 +00:08:38,380 --> 00:08:41,060 +للغاية شايفين الأسماء الأخيرة اللي موجودة على + +124 +00:08:41,060 --> 00:08:45,060 +اللوحة اللي هي penicillin, meticillin و vancobicin + +125 +00:08:45,060 --> 00:08:49,120 +اللي هون هي penicillin resistant بنسبة 95% ل 99% + +126 +00:08:49,120 --> 00:08:55,180 +في غزة عندنا هنا بنسبة من 95% ل 99% ال meticillin + +127 +00:08:55,180 --> 00:08:57,680 +وصلت ال resistance rate بعتها لأكتر من 40% + +128 +00:09:02,680 --> 00:09:06,680 +بواد الظهور ل Vancomycin-resistant Staphylococcus + +129 +00:09:06,680 --> 00:09:12,660 +aureus دراسة قبل أقل من شهر طلعت تلاتة و نص فالمية + +130 +00:09:12,660 --> 00:09:16,080 +يعني معناه الحاجة إن أنا فيها عندي مشكلة حقيقية + +131 +00:09:16,080 --> 00:09:18,880 +يعني هايجي يوم من الأيام لأنا استطيع أن أعالج + +132 +00:09:18,880 --> 00:09:21,040 +Staphylococcus aureus بال available drugs اللي + +133 +00:09:21,040 --> 00:09:24,380 +موجودة في الأسواق طبعا ال available drugs اللي هي + +134 +00:09:24,380 --> 00:09:30,560 +يمكن استخدامها استخدام للأدمالصورة اللي قدامنا هي + +135 +00:09:30,560 --> 00:09:33,200 +دي بتوضح ال hemolysis اللي حكينا عنه في المحاضرة + +136 +00:09:33,200 --> 00:09:36,920 +السابقة اللي هو beta .. beta hemolysis complete + +137 +00:09:36,920 --> 00:09:40,200 +hemolysis .. complete hemolysis الصورة هي بتوضح ان + +138 +00:09:40,200 --> 00:09:42,280 +ال .. ال .. مش ال hemolysis تعفو عنه ال manitol + +139 +00:09:42,280 --> 00:09:46,400 +salt agar هذا بده وقفة ويردت نتركز عنه شوية ال + +140 +00:09:46,400 --> 00:09:50,700 +manitol salt agar هو عبارة عن selective and + +141 +00:09:50,700 --> 00:09:54,120 +differential كلكوا بتعرف إيش أنا selective و إيش + +142 +00:09:54,120 --> 00:09:57,360 +أنا differentialالان كيف صار selective في هذا + +143 +00:09:57,360 --> 00:10:03,880 +الوساط؟ اضيف له 7.5% Sodium Chloride 7.5% Sodium + +144 +00:10:03,880 --> 00:10:09,520 +Chloride عارفين ايش يعني 7.5؟ يعني لما نحضر لتر + +145 +00:10:09,520 --> 00:10:14,980 +يعني 75 gram ده لتر يعني الملوحة تبعتها اعلى من + +146 +00:10:14,980 --> 00:10:21,320 +ملوحة البحر بضائفين تقريبا اعلى بقفل ضعف ملوحة + +147 +00:10:21,320 --> 00:10:25,860 +البحرمين ممكن ينمو في هذه ال concentration من ال + +148 +00:10:25,860 --> 00:10:29,260 +Sonium chloride لستفيلو الكوكاية؟ يبجأ المناعة + +149 +00:10:29,260 --> 00:10:34,280 +تنمو الميكروبات، الأخرى، مش بس ال Stripto، حتى + +150 +00:10:34,280 --> 00:10:37,280 +اللي امتلو بكترياسي المناعة تنموها لأنك لن تستطيع + +151 +00:10:37,280 --> 00:10:42,620 +تنمو في 7.5 يبجأ الوضع تصبح selective لمين؟ + +152 +00:10:42,620 --> 00:10:46,340 +للاستفيلو الكوكاية كيف صار ال differential؟ أضافنا + +153 +00:10:46,340 --> 00:10:52,510 +عليه Manetol ومع ال Manetol pH indicatorالان لون + +154 +00:10:52,510 --> 00:10:56,850 +الوسط الأصلي بنات شفين اللون الزهري هذا هو ده + +155 +00:10:56,850 --> 00:11:01,810 +اللون الأصلي للوسط لما نمنا عليه البكتيريا مين + +156 +00:11:01,810 --> 00:11:04,910 +اللي هنمو؟ ال Staphylococci اللي عندها مقدرة اللي + +157 +00:11:04,910 --> 00:11:10,790 +هتنمو عليها أعلى من 7.5% Sodium Chloride طيب ال + +158 +00:11:10,790 --> 00:11:14,010 +Staphylococci في منها نوعين نوع بيعمل Menitol + +159 +00:11:14,010 --> 00:11:17,230 +Fermentation زي ال Staphylococcus aureus شفت صورة + +160 +00:11:17,230 --> 00:11:19,730 +.. شفتوا التلاتة فتخلصوا اتحكيهم قبل شوية؟ منهم + +161 +00:11:19,730 --> 00:11:24,490 +Menitol Fermentationوباقي الستفيلو كوكاي are non + +162 +00:11:24,490 --> 00:11:30,110 +manitol fermenter إذا ال manitol fermenter بكتيريا + +163 +00:11:30,110 --> 00:11:33,930 +نمت على هذا الوسط هتخفض ال pH as a result of the + +164 +00:11:33,930 --> 00:11:38,010 +acid produced by manitol fermentation انخفاض ال pH + +165 +00:11:38,010 --> 00:11:45,290 +هيحول لون الوسط من زهري إلى أصفرإذا بتلاحظوا في + +166 +00:11:45,290 --> 00:11:48,690 +المثلثات اللي موجودة قدامكوا باللون الأصفر هذي دي + +167 +00:11:48,690 --> 00:11:52,450 +لا علاج على إن البكتيريا .. على إن البكتيريا منطال + +168 +00:11:52,450 --> 00:11:55,350 +.. خلّيني أكون متواضعين دايما في التعرف على + +169 +00:11:55,350 --> 00:11:59,530 +البكتيريا نحاول نوصف وصف دقيق، هي منطال fermented + +170 +00:11:59,530 --> 00:12:03,490 +إذا كنا عاملينها كتاليز، بنقول عنها كتاليز بصدر، + +171 +00:12:03,490 --> 00:12:06,270 +إذا كنا عاملين جرام استنقلها، هتكون جرام + +172 +00:12:10,450 --> 00:12:14,070 +يبقى الاحتمال الاكبر هتكون Staphylococcus aureus + +173 +00:12:14,070 --> 00:12:17,530 +اعتقد ان هذا الصورة واضحة، لذا هذا بسط رائع جدا + +174 +00:12:17,530 --> 00:12:23,490 +لعزل البكتيريا من بيئات تحتوي على أنواع اخرى من + +175 +00:12:23,490 --> 00:12:27,970 +البكتيريا، بيمنعلي ال others و بعرفني ل + +176 +00:12:27,970 --> 00:12:32,330 +Staphylococcus aureus، طب ناخدله تطبيقهناك بعض + +177 +00:12:32,330 --> 00:12:35,930 +الأغضية بنات، بعض الأغضية، من غير مسموح أن يكون + +178 +00:12:35,930 --> 00:12:40,770 +فيها Staphylococcus aureus إلا بعدد أقل من 100، من + +179 +00:12:40,770 --> 00:12:44,190 +100 لكل gram من الغذاء، فكيف أدي عادل + +180 +00:12:44,190 --> 00:12:47,550 +Staphylococcus aureus؟ بعدها على وساط selective + +181 +00:12:47,550 --> 00:12:52,790 +and differential زي المنطول، salt، عجب، ليش؟ لأنه + +182 +00:12:52,790 --> 00:12:59,850 +بيبنعل الميكروبات الأخرىو بيناميلها و بيميزها بلون + +183 +00:12:59,850 --> 00:13:01,830 +الأصفر اللي هو نتجة عنه اللي مانت هو ال + +184 +00:13:01,830 --> 00:13:05,250 +fermentation إذا من وضعات كتير بهم سبق لنا في + +185 +00:13:05,250 --> 00:13:11,010 +النظري أو في العمل فحص ال DNA's بندنظر للصورة + +186 +00:13:11,010 --> 00:13:14,610 +الأسفل هي الشائعة اللي هتستخدموها تركزوا معايا بس + +187 +00:13:14,610 --> 00:13:20,850 +شوية بالأول عشان نشرح الفكرة بتلعبوا ال DNA انتوا + +188 +00:13:20,850 --> 00:13:26,250 +ال DNA ايه؟في بعض الأغذية بنسميها DNA-rich foods + +189 +00:13:26,250 --> 00:13:34,150 +من ضمنها البقليات Serials الآن بيحطوا كمية من ال + +190 +00:13:34,150 --> 00:13:39,430 +soy beans اللي هي أحد البقليات هنا في الوسط يبقى + +191 +00:13:39,430 --> 00:13:44,290 +وسط مبني على soy beans مليان DNA مليان DNA الوسط + +192 +00:13:44,290 --> 00:13:46,810 +اللي لما كنتوا تستخدموه في المعمل لو طلعتوا على ال + +193 +00:13:46,810 --> 00:13:49,290 +Miller-Hinton أو على ال nutrient exam اللي بتلاقوه + +194 +00:13:49,290 --> 00:13:54,600 +شفافtransparent لإيش؟ لأنه مأخوذ من مواد ذائبة + +195 +00:13:54,600 --> 00:13:58,660 +والمواد الذائبة مابتعملاش terminity مابتعملاش + +196 +00:13:58,660 --> 00:14:03,160 +terminity ذائبة كلية الآن لو اضطررت على الوسط + +197 +00:14:03,160 --> 00:14:06,420 +العالي الطبيعي حتى يبقى شفاف زرعنا على البكتيريا + +198 +00:14:06,420 --> 00:14:11,600 +اللى بنافحصهاشكل نقطة مش ذراعية عادية نقطة اعملنا + +199 +00:14:11,600 --> 00:14:15,780 +نقطة و المستعمرة بدت تكبر و بعد 24 ساعة طلعنا على + +200 +00:14:15,780 --> 00:14:19,020 +الطبق زي ما هو ماقدرنا نعرف ان البكتيريا بتنتج DNA + +201 +00:14:19,020 --> 00:14:24,960 +او ماتنتجش DNA كيبنا نعرف ضفنا point واحد او واحد + +202 +00:14:24,960 --> 00:14:30,800 +مربع عفوا HCl ايش بيعمل HCl ال HCl بيعمل + +203 +00:14:30,800 --> 00:14:35,850 +denaturation لل proteinلما يعمل .. أفضل لل DNA .. + +204 +00:14:35,850 --> 00:14:40,090 +لما يعمل denaturation لل DNA بيعمل turbidity في + +205 +00:14:40,090 --> 00:14:46,810 +الوسط، لاحظوا الوسط هنا بطل clear، صح؟ وين فيه + +206 +00:14:46,810 --> 00:14:52,890 +clear؟ حوالين المستعمر هذه وحوالين المستعمر هذه، + +207 +00:14:52,890 --> 00:14:56,350 +لكن حوالين المستعمر هذه فيه clear؟ طب اللي فيها + +208 +00:14:56,350 --> 00:15:00,560 +clearليش فيها clear؟ لأنه مش فيها DNA و اراها ال + +209 +00:15:00,560 --> 00:15:05,020 +DNA تكسّر بفعل ال DNA's اللي قدرته البداية وصل + +210 +00:15:05,020 --> 00:15:09,760 +الصورة لأنه بيقدر نميز DNA's بُصلف عن DNA's + +211 +00:15:09,760 --> 00:15:14,580 +negative في وسط اسمه DNA's أجار باستخدام واحد + +212 +00:15:14,580 --> 00:15:19,000 +normal HCl متى بعد ما تخلص عملية ال incubation + +213 +00:15:19,000 --> 00:15:21,780 +انتوا عملتوا ال startشان دول لسه اللي لسه في + +214 +00:15:21,780 --> 00:15:22,340 +المعمل؟ + +215 +00:15:28,350 --> 00:15:31,850 +الألف أمياليزي فكرته أنه نزرع البكتيريا على وطط + +216 +00:15:31,850 --> 00:15:37,290 +فيه starch و بعد 24 ساعة بنعمل flooding للطبق بال + +217 +00:15:37,290 --> 00:15:41,830 +IOD's ال IOD's مش بيعمل بيرضبط مع ال starch بيكوّل + +218 +00:15:41,830 --> 00:15:45,390 +لون أزرق زي اللي زي المشهورة اللي أخدناها زمان + +219 +00:15:45,390 --> 00:15:49,310 +المنطقة اللي خالية من ال starch حضرها colorless + +220 +00:15:49,310 --> 00:15:54,800 +نفس الفكرةو أربع جنسها و بعدين نظفنا اللى هو one + +221 +00:15:54,800 --> 00:15:59,500 +normal HCL صار turbidity حول المستعمرة negative ما + +222 +00:15:59,500 --> 00:16:03,040 +صار turbidity حول المستعمرة الفحص positive الصور + +223 +00:16:03,040 --> 00:16:06,400 +اللى فوق تقنية بيستخدموا فيها indicator مختلف + +224 +00:16:06,400 --> 00:16:11,380 +فبالتالي ال interpretation بتكون مختلفة كمان نقطة + +225 +00:16:11,380 --> 00:16:14,220 +مهمية دلوقت لجوكاس اوريس او لبعض انواع من + +226 +00:16:14,220 --> 00:16:16,680 +البكتيريا اللى بتستخدم مركب + +227 +00:16:21,620 --> 00:16:25,720 +البوتاسيوم تليوريد مُركّب بصير له reduction من بعض + +228 +00:16:25,720 --> 00:16:28,700 +البكتيريا reduction يعني utilization يعني استخدام + +229 +00:16:28,700 --> 00:16:33,060 +مش كل البكتيريا تقدر تعمله reduction few bacteria + +230 +00:16:33,060 --> 00:16:36,860 +can produce this substance أو use this substance + +231 +00:16:36,860 --> 00:16:41,180 +منهم من الـ Staphylococcus aureus لما بتعمله + +232 +00:16:41,180 --> 00:16:44,680 +reduction ويكون في الوسط هو ويكون colorless بصير + +233 +00:16:44,680 --> 00:16:49,840 +لونه أسود فالمستعمرات بصير لونها أسود فبالتالي + +234 +00:16:49,840 --> 00:16:53,230 +برضه بميز مين؟الستافيلوكوكاس أو رياسة عن + +235 +00:16:53,230 --> 00:16:56,270 +الميكروبات الأخرى بلون المستعمر اللي هو اللون + +236 +00:16:56,270 --> 00:17:00,450 +الأسود اللي أنا بوصلكوا إياه ده رسالة إنه في أوساط + +237 +00:17:00,450 --> 00:17:04,410 +selective غير ال manitol salt agar وفكرة أخرى ده + +238 +00:17:04,410 --> 00:17:08,370 +اختلف عن ال manitol salt agar لكن معتمد على مين؟ + +239 +00:17:08,370 --> 00:17:11,570 +على ال potassium telluride ال manitol salt agar + +240 +00:17:11,570 --> 00:17:15,290 +بقى معتمد على مين؟ على ال sodium chloride المسبة + +241 +00:17:15,290 --> 00:17:20,030 +العالى اللي هي 7.5%هذا فحص جديد مش هتاخده في + +242 +00:17:20,030 --> 00:17:24,550 +المعمل، فياريت تطلعوا عليه كويس دايما أبدا بعد + +243 +00:17:24,550 --> 00:17:27,650 +نقطع شهود احنا في ال micro biology في معلومات + +244 +00:17:27,650 --> 00:17:32,470 +بتصير مسلنات بنحاول أنه نعطيكوا بعض ال exceptions + +245 +00:17:32,470 --> 00:17:35,810 +جرعات من ال exceptions، كويس أحسن من أنه نعطيكوا + +246 +00:17:35,810 --> 00:17:38,750 +ال exception في نفس اليوم لما كنا نقول + +247 +00:17:38,750 --> 00:17:43,210 +Staphylococcus و Stryptococcus عنوانهم ground + +248 +00:17:43,210 --> 00:17:46,250 +mustard coccyte احنا كنا الصحيح مانقولش كل الحقيقة + +249 +00:17:47,460 --> 00:17:52,580 +في كمان مجموعة .. في كمان مجموعة .. في كمان مجموعة + +250 +00:17:52,580 --> 00:17:57,340 +اسمها micrococcus micrococcus يبقى micrococcus, + +251 +00:17:57,520 --> 00:18:01,740 +staphylococcus و streptococcus ما المشترك بين ال + +252 +00:18:01,740 --> 00:18:06,100 +staphylococcus و ال micrococcus الكتاليزي انهم ال + +253 +00:18:06,100 --> 00:18:09,580 +ground boaster كوكساي و الكتاليزي بوستر كبنا ميز + +254 +00:18:09,580 --> 00:18:12,320 +بينهم هذا الفحص اللي قدامكوا اسمه ال isostaphine + +255 +00:18:13,730 --> 00:18:17,230 +Lysostafin واضح من اسمه إنه بيعمل lice لمين؟ + +256 +00:18:17,230 --> 00:18:22,510 +للاستاف، كل الاستاف بتدوب، كده إيش يعني بتدوب؟ + +257 +00:18:22,510 --> 00:18:25,950 +يعني بتموت و بتتحلل، بتموت و بتتحلل، أصلا ال + +258 +00:18:25,950 --> 00:18:29,150 +Benzaline عارفين كيف بيقتل البكتيريا اللي بيقتل + +259 +00:18:29,150 --> 00:18:32,990 +الاستافين الجوكاساوريا برضه بتتحللأصلا اكتشافه + +260 +00:18:32,990 --> 00:18:37,470 +البنسيلين من قبل Alexander Fleming كان بطريقة هذه + +261 +00:18:37,470 --> 00:18:40,430 +فطر نمى جنب ال .. ال .. الـ cephalopoccus aureus + +262 +00:18:40,430 --> 00:18:45,150 +دول المستعمرات اللي أصلا كانت موجودة فالبكتيريا + +263 +00:18:45,150 --> 00:18:48,950 +ممكن إذا ال cell wall تبعها ات .. اتحلل و أنتوا + +264 +00:18:48,950 --> 00:18:51,550 +عارفين البنسيلين مش تعملان أيه؟ على ال cell wall + +265 +00:18:51,550 --> 00:18:54,930 +طب كيف بيناعرف ان ال .. ال .. اتحلل .. البكتيريا + +266 +00:18:54,930 --> 00:18:57,830 +ولا اتحللت ايش؟ طلع على انبوبة و هذا .. هذا يعني + +267 +00:18:57,830 --> 00:19:01,910 +نقطة كتير مهمة بنستخدمها احنافي ال comparisons + +268 +00:19:01,910 --> 00:19:09,230 +بنجيب مثلا ورقة مطبوعة تمام و بنحط أمبوبتين قدام + +269 +00:19:09,230 --> 00:19:14,730 +الورقة و بنقرأ الكلام المطبوع من خلال الأنابيب في + +270 +00:19:14,730 --> 00:19:17,470 +هذا الحالة ما بنقرأش احنا قاعدين هنا نقرأ وضوح + +271 +00:19:17,470 --> 00:19:21,350 +الخطوط فقط ما بنقرأش كلام نقرأ وضوح الخطوط مين + +272 +00:19:21,350 --> 00:19:26,830 +أوضح؟ واضح انه هنا الخط واضح جدا بينما هنا يعني ال + +273 +00:19:26,830 --> 00:19:29,370 +turbidity هنا أعلى من ال turbidity هنا + +274 +00:19:45,260 --> 00:19:50,300 +مؤشر على أن البكتيريا اللي نميها هنا لم تتحلل ماذا + +275 +00:19:50,300 --> 00:19:53,280 +يعني ذلك؟ انها resistant ماذا يعني ذلك؟ + +276 +00:19:53,280 --> 00:20:00,680 +micrococcus فوق البكتيريا فقدت تماما من الأنبوبة + +277 +00:20:01,950 --> 00:20:06,190 +وبتالي هى sensitive وبتالي هى stiff وصلت الصورة + +278 +00:20:06,190 --> 00:20:10,490 +هذا فحص كتير مهم لانه بيميز لبن two organisms + +279 +00:20:10,490 --> 00:20:16,430 +بيشبهوا بعض بدرجة كبيرة او درجات الشبه غير ان هم + +280 +00:20:16,430 --> 00:20:19,590 +جرام بوستد كوكساي وكيتاليز بوستد كمان على الطبق + +281 +00:20:19,590 --> 00:20:24,050 +they may look similar يعني الاستافيلو كوكس + +282 +00:20:24,050 --> 00:20:28,070 +والمايكرو كوكس ممكن يكونوا أشكال مستعملات قريبة من + +283 +00:20:28,070 --> 00:20:33,810 +بعضهم البعض فواجب علينا ان نحن نميز بيننجي لـ .. + +284 +00:20:33,810 --> 00:20:40,290 +ال .. ال .. ال .. ال rapid tests في المعمل + +285 +00:20:40,290 --> 00:20:44,330 +هتشتغلوا اندول بأنبوبة و Methyl Red في أنبوبة و + +286 +00:20:44,330 --> 00:20:46,810 +Vagos Blitzkrieg في أنبوبة و Citrate في أنبوبة و + +287 +00:20:46,810 --> 00:20:50,450 +Motility في أنبوبة يعني لو بدي أعمل عشرين فحص أنا + +288 +00:20:50,450 --> 00:20:53,690 +بدي عشرين أنبوبة بديراك هالجدي و أنا بيب تقريبا + +289 +00:20:53,690 --> 00:20:58,970 +عشرة صندوق طولها أو أكتر يعني عشان أعمل مجموعة .. + +290 +00:20:58,970 --> 00:21:02,530 +م .. م .. فحصات لمجموعة بكتيريا بعب ال incubator + +291 +00:21:05,330 --> 00:21:09,890 +فالشركات لجأت إلى ما يسمى بال miniaturization يعني + +292 +00:21:09,890 --> 00:21:15,630 +لكبير نخليه لصغير زي ما بنعمل مجسمات لعمارة + +293 +00:21:15,630 --> 00:21:18,850 +الجدران احنا عملنا مجسمات لفحوطات صغيرة بس مجسمات + +294 +00:21:18,850 --> 00:21:24,270 +حقيقية و فعالة شغالة تمام شايفين الشريط هذا اللي + +295 +00:21:24,270 --> 00:21:28,810 +قدامنا اسمه ABI وانسوا الباقي ABI Analytical + +296 +00:21:28,810 --> 00:21:32,930 +Profile Index من شركة فرنسية مشهورة اسمها Biomero + +297 +00:21:34,600 --> 00:21:40,360 +entered مجموعة من ال strips هذه لمجموعات مختلفة من + +298 +00:21:40,360 --> 00:21:45,440 +البكتيري يعني مثلا عنا ABI-20E للانتروبكترياسي + +299 +00:21:45,440 --> 00:21:50,040 +والنانثارمينتر عنا ABI-staph للستافيرو كوكس عنا + +300 +00:21:50,040 --> 00:21:54,340 +ABI-strip للستريبتو كوكس عنا ABI-anaerobes + +301 +00:21:54,340 --> 00:21:58,260 +للبكتيرى اللى هوائية عنا ABI-nyseria تبقى فينا + +302 +00:21:58,260 --> 00:22:03,270 +أنواع مختلفة من ايه؟ من ال ABI ال ABI هو مسمىللـ + +303 +00:22:03,270 --> 00:22:07,890 +system نفسه لكن ال system موزع على أنواع مختلفة من + +304 +00:22:07,890 --> 00:22:11,810 +اللي هو البكتيريا طيب يالا بدنا نحفظ ال positive و + +305 +00:22:11,810 --> 00:22:17,990 +ال negative تبعات هذولة بتقدروا تحفظهموا؟ تقدروا؟ + +306 +00:22:17,990 --> 00:22:23,170 +طيب لما أجيبلكوا كمان 200 .. 200 .. 200 بكتيريا ب + +307 +00:22:23,170 --> 00:22:28,650 +20 فحص هتكون العملية يعني معقدة فانا مضطر أن ألجأ + +308 +00:22:28,650 --> 00:22:30,650 +إلى ال internet قرر أن يكون هناك internet + +309 +00:22:34,650 --> 00:22:41,530 +القاعة في برنامج اسمه ID backed سجله اسمه ID + +310 +00:22:41,530 --> 00:22:42,090 +backed + +311 +00:22:48,000 --> 00:22:53,960 +الفايل اللي مش موجود انا هنزلكه نسخة على الفيسبوك + +312 +00:22:53,960 --> 00:22:57,520 +لكن عشان تقدر تنزله على ويندوز حديث هذا المصمم كان + +313 +00:22:57,520 --> 00:23:01,600 +لويندوز اكس بي لازم تتطلعوا على اليوتيوب هذا عشان + +314 +00:23:01,600 --> 00:23:05,700 +تقدروا تعرفوا كيف بنعمل عملي التوافق للبرنامج وكيف + +315 +00:23:05,700 --> 00:23:07,140 +بنشغله هذا انا قعدت + +316 +00:23:18,290 --> 00:23:23,850 +هو المفروض انه بنزل بكل سهولة من الانترنت بعد + +317 +00:23:23,850 --> 00:23:24,330 +الشكل + +318 +00:23:32,000 --> 00:23:35,380 +عادة من كلكم قبولها بتنزيل الملفات وتشغيلها أو + +319 +00:23:35,380 --> 00:23:38,580 +تعمل set up يعني next next مافيش أي مشكلة في + +320 +00:23:38,580 --> 00:23:41,680 +الموضوع بس تعرفون بتخزنوا الملف تبعكم و بعدين + +321 +00:23:41,680 --> 00:23:44,720 +تعملوا set up المشكلة في set up في برامج من النوع + +322 +00:23:44,720 --> 00:23:50,840 +هذا الدعم الفني توقف عند ال xp و التالي بتدعمش + +323 +00:23:50,840 --> 00:23:55,440 +برامج اللي هم ال .. ال .. ال vista و ال seven و ال + +324 +00:23:55,440 --> 00:23:58,520 +eight في ال windows عشان تقدر تشتغل عليها لازم + +325 +00:23:58,520 --> 00:24:00,660 +نعمل حركة الحين بنشوفها كمان شوية + +326 +00:24:04,090 --> 00:24:08,310 +هنا بس عاملا بنختار ال location لتنزيل الملف ملف + +327 +00:24:08,310 --> 00:24:24,990 +صغير جدا لا يستغل ثواني في التحميل ناخده + +328 +00:24:24,990 --> 00:24:28,870 +نحطه على ال D هاي البرنامج + +329 +00:24:39,130 --> 00:24:47,550 +استكشاف مشاكل التوافق واصلاحها او خصائص تشوفت + +330 +00:24:47,550 --> 00:24:55,370 +التوافق؟ نختار تشغيل في وضع التوافق على ويندوز + +331 +00:24:55,370 --> 00:24:59,710 +جديد مثل ويندوز اكس + +332 +00:24:59,710 --> 00:25:05,570 +بي توإذا ماعملش الحركة دى مش هيشتغل البرنامج مش + +333 +00:25:05,570 --> 00:25:12,350 +هيشتغل البرنامج الآن خلاص هو جاهز للتشغيل بنشغله + +334 +00:25:12,350 --> 00:25:18,850 +كمسئول ك administrator يعني + +335 +00:25:18,850 --> 00:25:24,970 +هاي + +336 +00:25:24,970 --> 00:25:28,690 +set up زى ما حكالكوا next next مافيش حاجة special + +337 +00:25:28,690 --> 00:25:34,390 +في الموضوعو خلال ثانية واحدة تقريبا بيكتمل لغتات + +338 +00:25:34,390 --> 00:25:43,070 +تبعته هاي + +339 +00:25:43,070 --> 00:25:50,910 +و الآن صار في قائمة البرنامج طبعا + +340 +00:25:50,910 --> 00:25:54,890 +البرنامج فيه إمكانيتين فيه إمكانية استخدام التعرف + +341 +00:25:54,890 --> 00:25:56,190 +قائمة التعرف + +342 +00:26:00,040 --> 00:26:04,480 +و فيه هو أصلا databases قواعد بيانات مجهزة و مجهزة + +343 +00:26:04,480 --> 00:26:09,100 +زي ال ABI 10 ال ABI 10 إذا بنفتح بهذه الطريقة + +344 +00:26:09,100 --> 00:26:12,880 +بيعطينا boxes شايفين الصناديق هذه بنعبيها ال + +345 +00:26:12,880 --> 00:26:14,980 +positive و ال negative تبعنا اللي احنا حصلنا عليه + +346 +00:26:14,980 --> 00:26:17,820 +في المعمل من ال strip positive negative negative + +347 +00:26:17,820 --> 00:26:20,940 +positive حسب النتائج اللي طلعت معاناوبضغطة واحدة + +348 +00:26:20,940 --> 00:26:25,020 +enter بيعطينا اسم ال microbe ويبقونا جداش نسبة + +349 +00:26:25,020 --> 00:26:30,900 +التعرف بدقة على هذا ال microbe مهم جدا انه نعرف + +350 +00:26:30,900 --> 00:26:35,560 +كيف نتعامل مع هذه البرامج لإنها جزء من المستقبل + +351 +00:26:35,560 --> 00:26:41,680 +تبعنا في ال microbe لحظة ال green واصل part + +352 +00:26:41,680 --> 00:26:44,760 +لتقريبا 99% فبقى انا احكي ال identification تبعت + +353 +00:26:44,760 --> 00:26:49,280 +هي قوية ومعرفة على انها vibrio choleraباقي انا + +354 +00:26:49,280 --> 00:26:55,580 +اذكر كلمة واحدة هذه أجهزة برامج غبيه سريعه احنا + +355 +00:26:55,580 --> 00:26:57,680 +اللي بنلقنها نعم احنا اللي بنعطيها المعلومات + +356 +00:26:57,680 --> 00:27:02,820 +وبترجلنا بس بترجلناها بسرعة فإذا انت شغل غلطو + +357 +00:27:02,820 --> 00:27:05,860 +اعطته اي حاجة انا دخلت من عندى positive negative + +358 +00:27:05,860 --> 00:27:09,020 +positive و اعطانى اجابة عسب قواعد البيانات اللى + +359 +00:27:09,020 --> 00:27:12,760 +عنده فلازم نشتغل صح عشان لما ادخل صح يعطينا هو + +360 +00:27:12,760 --> 00:27:17,420 +برضه ايضا تعريف صح هذا احد البرامج اللى اسمه بنا + +361 +00:27:17,420 --> 00:27:23,300 +ID بقى سهل جدا فى التعامل معاه ويمكن اضافة قواعد + +362 +00:27:23,300 --> 00:27:27,020 +بيانات من عندنا احنا نعمل قواعد بيانات خاصة فينا + +363 +00:27:27,020 --> 00:27:30,100 +وندخلها يعني لو انا اتفهم ان انا اتطورت system + +364 +00:27:30,100 --> 00:27:34,740 +جديدمش ال ABI-20 عملت 10 فحصات بدل 20 فحص سبعة + +365 +00:27:34,740 --> 00:27:38,520 +فحصات بدل 20 فحص ممكن أدخلهم أنا أعمل قاعدة بيانات + +366 +00:27:38,520 --> 00:27:42,100 +و أدخلهم وصيلة استخدامها في التعرف على ال micro + +367 +00:27:42,100 --> 00:27:48,420 +organism طيب نشوف اللي .. اللي قلنا عنه ال PEP1 + +368 +00:27:48,420 --> 00:27:54,580 +أنا + +369 +00:27:54,580 --> 00:27:58,640 +بحب دايما أضيف كلمة bacteria عشان مايجيبوش هدا + +370 +00:27:58,640 --> 00:27:59,960 +التانية غير متوقعة + +371 +00:28:03,160 --> 00:28:06,480 +بروبولاستيك هو اختصار البروبولاستيك identification + +372 +00:28:06,480 --> 00:28:07,960 +of bacteria for windows + +373 +00:28:16,120 --> 00:28:19,740 +من اجمل .. من اجمل برامج لل .. لل researchers مش + +374 +00:28:19,740 --> 00:28:22,700 +ال practical صحيح لل .. لل .. لل عاملين لكن اللي + +375 +00:28:22,700 --> 00:28:25,880 +بيحب يتعرف على الميكروبات المجهولة من اي source من + +376 +00:28:25,880 --> 00:28:28,920 +اي مصدر سواء بيئي او طبية و كده بيستخدم هذا + +377 +00:28:28,920 --> 00:28:31,920 +البرامج انا بانصح في طلابي الملستير اللي بيستخدموا + +378 +00:28:31,920 --> 00:28:37,100 +بكتيريا غير .. او مجهولة هم مش عارفينها يعني + +379 +00:28:37,100 --> 00:28:40,720 +بيدوروا على خاصية فيها مش على اسمها و بدهم لما + +380 +00:28:40,720 --> 00:28:45,740 +لاقوا الخاصية يتعرفوا على الاسم بعد ذلك فممكن ناخد + +381 +00:28:46,380 --> 00:28:50,100 +نعمل download و ندور على المصفوفات اللي هي قواعد + +382 +00:28:50,100 --> 00:28:53,660 +البيانات و عنده قائمة محترمة جدا من المصفوفات كلها + +383 +00:28:53,660 --> 00:28:58,420 +for free يعني برنامج for free بيساعدنا في التعرف + +384 +00:28:58,420 --> 00:29:02,170 +على ال micro organismهنا اللي خدعناه ان نتكلم عن + +385 +00:29:02,170 --> 00:29:05,370 +البرامج اللي مستخدمة في التعرف على البكتيريا هو + +386 +00:29:05,370 --> 00:29:10,330 +وجود أنظمة زي ال ABI صحيح سهلت علينا لكن في نفس + +387 +00:29:10,330 --> 00:29:14,690 +الوجود ذات اللي على الذاكرة البشرية وبتالي اضطررنا + +388 +00:29:14,690 --> 00:29:18,830 +نلجأ للبرامج المحاوظبة اللي بتساعدنا بشكل كبير في + +389 +00:29:18,830 --> 00:29:20,770 +التعرف على هذه الميكروفر + +390 +00:29:25,460 --> 00:29:28,120 +أحنا يعني طول الفترة السابقة أتكلم على + +391 +00:29:28,120 --> 00:29:31,340 +Staphylococcus aureus المكورة الأنقضية الذهبية + +392 +00:29:31,340 --> 00:29:37,920 +إلها أخوات مهمين زي Staphylococcus epidermidis و + +393 +00:29:37,920 --> 00:29:44,420 +Staphylococcus saprophytic لكن اللي بدأ أتكلم عنه + +394 +00:29:44,420 --> 00:29:50,340 +الآن أنه بكفي أنه نخدم حق ل Staphylococcus + +395 +00:29:50,340 --> 00:29:54,680 +epidermidis و Staphylococcus saprophyticكنا نقول + +396 +00:29:54,680 --> 00:29:57,900 +إن الأورياس هي البعثوجين الوحيد في كل + +397 +00:29:57,900 --> 00:30:01,860 +Staphylococcus بعد فترة كنا لأ الأورياس ال .. ال + +398 +00:30:01,860 --> 00:30:05,260 +Epidermis بتسبب Endocarditis و بتسبب التهيبات ممكن + +399 +00:30:05,260 --> 00:30:09,060 +في صممات القلب السابروفايتكاس اللي جاية التهياب + +400 +00:30:09,060 --> 00:30:11,800 +منه سيستايتس اللي هي التهياب المثاني التهياب + +401 +00:30:11,800 --> 00:30:16,100 +المثاني طب الآن لو دورته على جوجل و كتبته + +402 +00:30:16,100 --> 00:30:19,200 +Coagulase Negative Staphylococcus و مين بينتمي على + +403 +00:30:19,200 --> 00:30:22,080 +Coagulase Negative Staphylococcus؟ التنتهي، مصبوف؟ + +404 +00:30:23,270 --> 00:30:28,330 +هتلاجوا أنه وصلوا مراتب في بعض الأحيان أعلى من الـ + +405 +00:30:28,330 --> 00:30:31,370 +Staphylococcus aureus في نسبة احداثهم للأمراض + +406 +00:30:31,370 --> 00:30:37,850 +المستشفيةالـ Coagulase Negative Staphylococci نظرة + +407 +00:30:37,850 --> 00:30:40,350 +مختلفة عن السابق يعني الـ Coagulase Negative + +408 +00:30:40,350 --> 00:30:43,550 +Staphylococci أصبحت تنافس الـ Coagulase Positive + +409 +00:30:43,550 --> 00:30:46,910 +Staphylococci وأنا بقولكوا أكتر من دراسة منها في + +410 +00:30:46,910 --> 00:30:50,050 +غزهان اللي جيناه بالفعل في بعض الأقسام في + +411 +00:30:50,050 --> 00:30:53,610 +المستشفيات الـ Coagulase Negative Staphylococci + +412 +00:30:53,610 --> 00:30:57,090 +كانت أعلى من اللي هو الـ Staphylococcus aureus فهي + +413 +00:30:57,090 --> 00:31:03,030 +في صعودوالصعود تبعها ناجم عن إنه هي تمتلك مؤهلات + +414 +00:31:03,030 --> 00:31:07,470 +وزادت مؤهلاتها أكتر من أحصار ال resistance لل + +415 +00:31:07,470 --> 00:31:10,810 +antibiotic وبالتالي انتشارها في المستشفيات بيكون + +416 +00:31:10,810 --> 00:31:14,730 +واسع أنطق Major component اسمها Epidermis على + +417 +00:31:14,730 --> 00:31:17,450 +الجذب موجودة فهي major component of skin flora + +418 +00:31:17,450 --> 00:31:21,870 +opportunistic infection لكن ال opportunistic كانت + +419 +00:31:21,870 --> 00:31:26,870 +بتزيد تذكروا دايما المستشفى بيه ألمين؟ للمرضى + +420 +00:31:26,870 --> 00:31:30,270 +للمرضى يعني لمين؟ده اللي ال immune system عندهم + +421 +00:31:30,270 --> 00:31:33,750 +immunocompromised وبالتالي الميكروبات ال + +422 +00:31:33,750 --> 00:31:36,610 +opportunistic قادر ان يتعمل infection عند هذا + +423 +00:31:36,610 --> 00:31:39,770 +الناس ال heart valves وقلنا فيها ان ال + +424 +00:31:39,770 --> 00:31:42,310 +endocarditis يعني من الأمراض المشهورة لهذه + +425 +00:31:42,310 --> 00:31:45,790 +البكتيريا ال identification ما بتعملاش يملحظ على + +426 +00:31:45,790 --> 00:31:49,530 +blood agar does not ferment manitol non pigmented + +427 +00:31:49,530 --> 00:31:55,680 +و QMAs طبعا نجات لحظوا النقاطمالكتوبرت فيرماتيشي + +428 +00:31:55,680 --> 00:31:59,000 +نيجاتيف و Coagulase نيجاتيف في كمان نقطة تالتة + +429 +00:31:59,000 --> 00:32:03,920 +DNA's نيجاتيف DNA's نيجاتيف لإن ما تلاقى ال + +430 +00:32:03,920 --> 00:32:09,640 +Staphylococcus aureus التلاتة بسيطة نيجي للميكروب + +431 +00:32:09,640 --> 00:32:13,040 +التاني اللي هو ال Sabrophytic و ال Sabrophytic + +432 +00:32:13,040 --> 00:32:17,400 +الصحيح يعني إرتباطه من لحد الآن في نوع واحد من + +433 +00:32:17,400 --> 00:32:20,160 +الأمراض اللي هو ال adrenaline tract infection و + +434 +00:32:20,160 --> 00:32:25,700 +بالأخرى ال cystitis اللي هي التهاب المثالطبعا من + +435 +00:32:25,700 --> 00:32:30,680 +ميزاته انه هو stuff كل صفات ال stuff عنده ما عدا + +436 +00:32:30,680 --> 00:32:34,680 +ال coagulase نجاتف طبعا و ال menitol fermentation + +437 +00:32:34,680 --> 00:32:39,380 +نجاتف ايضا و ال DNA نجاتفطب هذا بيصير هو و ال + +438 +00:32:39,380 --> 00:32:42,460 +Staphylococcus epidermidis في نفس الخانة كيبنا + +439 +00:32:42,460 --> 00:32:47,600 +ملزم عن بعض بيفحص اسمه Novobiocin ال Novobiocin هو + +440 +00:32:47,600 --> 00:32:49,880 +يبقى عن ال antibiotic يعني يبقى عن ال antibiotic + +441 +00:32:49,880 --> 00:32:53,640 +بنستخدمه في ال differentiation between saprophytic + +442 +00:32:53,640 --> 00:32:59,820 +acid resistant و ال epidermis بتكون sensitive طبعا + +443 +00:32:59,820 --> 00:33:03,870 +الصورة اللي قدامنا نعرض الصورةبتوضح ان القُرص + +444 +00:33:03,870 --> 00:33:07,050 +الانتيباتيكي اللي هو نوغروفايوسين و حواليه zone of + +445 +00:33:07,050 --> 00:33:10,650 +inhibition منطقة خالية من النمو ده لإنه البكتيريا + +446 +00:33:10,650 --> 00:33:14,230 +اللي هنا sensitive بينما في الجزء التاني من الطبق + +447 +00:33:14,230 --> 00:33:18,130 +مافيش zone of inhibition وبالتالي البكتيريا resist + +448 +00:33:18,130 --> 00:33:21,670 +طيب + +449 +00:33:21,670 --> 00:33:24,490 +نيجي لجزء التاني من الموضوع اللي هو + +450 +00:33:28,700 --> 00:33:32,860 +بنطلقها على تنتين في أكتر من السلاسل يعني لو كانوا + +451 +00:33:32,860 --> 00:33:40,340 +تنتين برضه بنسميهم streptococci صور + +452 +00:33:40,340 --> 00:33:43,100 +توضحية هذا الصور اللي بيقولنا صورة توضحية مش صورة + +453 +00:33:43,100 --> 00:33:46,280 +حقيقية هي عبارة عن رسم من تحت ال microscope لكن + +454 +00:33:46,280 --> 00:33:51,980 +بتوضح لنا إن ال chains طولها + +455 +00:33:51,980 --> 00:33:56,080 +مختلف قد تكون مكوّنة من تنتين بالفعلو هذا حسب ال + +456 +00:33:56,080 --> 00:34:00,160 +handling ال specimen يعني لو أنا أخدت أنبوبة و + +457 +00:34:00,160 --> 00:34:06,740 +زرعت فيها stirptococcus by genes أنبوبة liquid و + +458 +00:34:06,740 --> 00:34:11,820 +ماعملتلاش أي disturbance ماحركتاش خالص بعد 24 ساعة + +459 +00:34:11,820 --> 00:34:16,820 +لو يطلعت عليها بيلاجيها عملة النمو تبعها يشبه ما + +460 +00:34:16,820 --> 00:34:19,680 +يسمى بال cotton ball cotton ball يعني زي كرات + +461 +00:34:19,680 --> 00:34:25,590 +بالقطنكورة القطن من أيش كورة القطن؟ تخيلوا سلسلة + +462 +00:34:25,590 --> 00:34:30,810 +جالة تطول، إيه بتطول؟ في الآخر بتبقى لها مسكة حلوة + +463 +00:34:30,810 --> 00:34:36,830 +لك و لا تنحني؟ تنحني فبصير تلف و تشربك بع بعضها و + +464 +00:34:36,830 --> 00:34:40,390 +بتعملي زي إيه؟ زي الكورة، لو أخدت قطرة بهدوء من + +465 +00:34:40,390 --> 00:34:44,990 +أوحة تحت الميجروسكوب هلاجي very long .. very long + +466 +00:34:44,990 --> 00:34:49,780 +chainsيبقى طول السلسلة معتمدة على وين نمت + +467 +00:34:49,780 --> 00:34:52,600 +البكتيريا، إيش الظروف اللي اتعرضت إلها، وعملتي ال + +468 +00:34:52,600 --> 00:34:56,260 +handling، ال handling اللي هي و أنا بضرع فيها، أو + +469 +00:34:56,260 --> 00:34:58,220 +عفوا مش بضرع فيها، بعمل general staining، مش بعمل + +470 +00:34:58,220 --> 00:35:01,720 +mixing أنا، ال mixing هذا هو برضه بيكسر اللي هو ال + +471 +00:35:01,720 --> 00:35:07,240 +.. ال .. البكتيريامذكركم بال beta alpha gamma + +472 +00:35:07,240 --> 00:35:10,620 +hemolysis احنا في الاستخدام الكوكاس اولى استخدامنا + +473 +00:35:10,620 --> 00:35:12,900 +صفة ال beta hemolysis مرة واحدة اذا كنا beta + +474 +00:35:12,900 --> 00:35:16,480 +hemolytic او non-beta hemolytic بس بدكم صحيح ال + +475 +00:35:16,480 --> 00:35:21,740 +hemolysis اكتر استخدام له في الstriptococciيعني + +476 +00:35:21,740 --> 00:35:27,080 +أقولكوا كمان تعبير أدق قد نصف البكتيريا في الـ + +477 +00:35:27,080 --> 00:35:29,700 +streptococcus بالـ hemolysis تبعها، عفاش ما بنقول + +478 +00:35:29,700 --> 00:35:33,040 +Alpha hemolytic streptococci Beta hemolytic + +479 +00:35:33,040 --> 00:35:38,160 +streptococci Gamma hemolytic streptococci يبقى + +480 +00:35:38,160 --> 00:35:41,480 +عملية تصنيف الـ streptococci دخل فيها مين؟ الـ + +481 +00:35:41,480 --> 00:35:43,640 +hemolysis على الـ blood vessels، يعني ده فيها صفة + +482 +00:35:43,640 --> 00:35:48,380 +من الصفات المهمة جدا في الميكروبيل دي أرفك و تدفأ + +483 +00:35:48,380 --> 00:35:51,440 +نقلب، و هذه الصفة شاركت مع مين؟مع ال + +484 +00:35:51,440 --> 00:35:54,540 +Staphylococcus Gram-positive chains or pairs + +485 +00:35:54,540 --> 00:35:59,840 +كتاليز نيجاتيف وبين بسين Staphylococci are كتاليز + +486 +00:35:59,840 --> 00:36:05,140 +بوسطف مش معنى ال Staphylococci وهي facultative عن + +487 +00:36:05,140 --> 00:36:10,600 +أيروب كتاليز بوسطف ليش ال Stryptococci ما تكون + +488 +00:36:10,600 --> 00:36:12,960 +زيها كتاليز بوسطف ما هي facultative عن أيروب و + +489 +00:36:12,960 --> 00:36:17,940 +بتتحمل وجود ال oxygenبقول إن النظام الموجود فيها + +490 +00:36:17,940 --> 00:36:22,280 +بيختلف عن النظام الموجود في الـ Staphylococci في + +491 +00:36:22,280 --> 00:36:26,500 +الـ Staphylococci فيها SOD .. إيش ال SOD؟ Super + +492 +00:36:26,500 --> 00:36:31,620 +Oxide Desmutase زائد كيتاليز بينما في ال + +493 +00:36:31,620 --> 00:36:36,740 +Striptococci فيها SOD زائد Peroxidase خدوا بالكوا + +494 +00:36:37,110 --> 00:36:41,950 +البروكسديس والكتاليز يقوموا بنفس الوظيفة تحويل ال + +495 +00:36:41,950 --> 00:36:48,530 +H2O2 إلى ميه زائد أكسجين بس في الكتاليز الأكسجين + +496 +00:36:48,530 --> 00:36:53,810 +بيطلع كموليكلر أكسجينفي البروكسيدايز الأكسجين + +497 +00:36:53,810 --> 00:36:57,670 +بيستخدم مباشرة في ال reduction لمركبات تانية + +498 +00:36:57,670 --> 00:37:00,990 +فبيظهرش على أنه molecular أكسجين بجانب الفحص لو + +499 +00:37:00,990 --> 00:37:05,230 +حطينا بكتيريا عندها بيروكسيدايز على هيدروجين + +500 +00:37:05,230 --> 00:37:09,330 +بيروكسايد هل هيطلع bubbles؟ مش هيطلع bubbles لأن + +501 +00:37:09,330 --> 00:37:12,310 +مافيش فيها كتاليز فيها بيروكسيدايز + +502 +00:37:15,510 --> 00:37:18,210 +اللوحة الصعبة اللي هنوجب في عندها محاضرتنا في + +503 +00:37:18,210 --> 00:37:21,790 +الآخر لانه مش هنقدر نخلصها في المحاضرة في الوقت + +504 +00:37:21,790 --> 00:37:25,570 +المتبقى فانديها اسمها Stryptomycin و ال + +505 +00:37:25,570 --> 00:37:30,030 +Stryptomycin two types مكتوب عليهم واحد + +506 +00:37:30,030 --> 00:37:34,350 +Stryptomycin S واحد اسمه Stryptomycin O تعالوا + +507 +00:37:34,350 --> 00:37:38,070 +نطلع مع بعض على الصفورة و نشوف ايش يعني + +508 +00:37:38,070 --> 00:37:38,990 +Stryptomycin + +509 +00:37:45,050 --> 00:37:50,590 +O و Structurizing S + +510 +00:37:50,590 --> 00:37:55,490 +من أين جاءت أحرف هذه الكلمات من أين جاءت؟ تركزوا + +511 +00:37:55,490 --> 00:38:06,950 +معايا الاتنين لهم خاصية معينة تجاه الأكسجين هذا + +512 +00:38:06,950 --> 00:38:10,890 +الأول Oxygen Labia + +513 +00:38:13,400 --> 00:38:18,340 +يعني بيتدمر، بيتأثر بالـ Oxygen بينما التاني + +514 +00:38:18,340 --> 00:38:23,420 +Stable الاحرف + +515 +00:38:23,420 --> 00:38:30,140 +Oxygen ل Bi و هذه Oxygen A Stable طبعا التسمية + +516 +00:38:30,140 --> 00:38:35,780 +ممكن تكون مهمة، لكن برضه مش هي اللي المقصودة عنها + +517 +00:38:35,780 --> 00:38:41,860 +الـ Structural ICO لما + +518 +00:38:41,860 --> 00:38:45,230 +بدخل هذاالاسم الإنسان من ضمن البكتيريا اللي + +519 +00:38:45,230 --> 00:38:50,170 +بتنتجه، اسمنا بيكوّله Antibodies، إيش بنسميه + +520 +00:38:50,170 --> 00:38:56,110 +Antibodies؟ Antistreptolysin، او .. إذا + +521 +00:38:56,110 --> 00:39:00,030 +streptolysin هو مين؟ هو الاندجين، وال Antibody + +522 +00:39:00,030 --> 00:39:04,310 +اسمه Antistreptolysin، مين اللي بنتجه أساسا؟ + +523 +00:39:09,130 --> 00:39:12,390 +The streptococcus biogenes number one cause of + +524 +00:39:12,390 --> 00:39:17,590 +bacterial tonsillitis فاكرين + +525 +00:39:17,590 --> 00:39:21,910 +تونسولايتس؟ فيه هدافيكو يعني هرب منها في يوم من + +526 +00:39:21,910 --> 00:39:26,210 +الأيام خاصة في سن الطفولة معظمنا وصيب بيها مرارا + +527 +00:39:26,210 --> 00:39:30,890 +وتكرارا مرارا وتكرارا يبجى بتوقع أنه دخل عندنا ميم + +528 +00:39:30,890 --> 00:39:36,960 +SO واتكون عندنا ASOيعني لو فحصناك و الأن كلكوا + +529 +00:39:36,960 --> 00:39:40,500 +هلاجة أنكوا ASO هذا لا يعني أنكوا الأن مصابين + +530 +00:39:40,500 --> 00:39:46,060 +بالاستخدام وبالتالي اضطرينا أن نلجأ إلى حيلة العد + +531 +00:39:46,060 --> 00:39:51,040 +أو قياس التركيز فضفنا حرف ال T في الآخر صار اسم + +532 +00:39:51,040 --> 00:39:57,540 +الفحص ASOT و T معناها title و title معناها + +533 +00:39:57,540 --> 00:40:02,340 +concentrationوبالتالي الفحص بطل يقول positive + +534 +00:40:02,340 --> 00:40:07,080 +نيجاتين صار يجيس يعني بيقيس كمية اللي هو ال + +535 +00:40:07,080 --> 00:40:10,900 +antibodies لل Streptolysin وبالتالي اسم الفحص الآن + +536 +00:40:10,900 --> 00:40:17,660 +اسمه Anti Streptolysin O طيب بيجيس تركيز هل هناك + +537 +00:40:17,660 --> 00:40:20,720 +علاقة بين التركيز وبين وجود إصابة حالية أو إصابة + +538 +00:40:20,720 --> 00:40:25,620 +سابقة نعم يوجد إذا كان إصابة سابقة غالبا التركيز + +539 +00:40:25,620 --> 00:40:32,110 +بيكون أقل من 200 انتقاش نجنة per mlإذا كان إصابة + +540 +00:40:32,110 --> 00:40:40,330 +حالية غالبا فيكون أكتر من 200 يبقى الآن صراحة نعرف + +541 +00:40:40,330 --> 00:40:44,130 +الميز ما بين إصابة حالية وإصابة صادفة لإنه كان + +542 +00:40:44,130 --> 00:40:49,530 +بالأول صعب صعب جدا علينا نعرف لإن كل الناس أو معظم + +543 +00:40:49,530 --> 00:40:53,030 +الناس أصيبوا في يوم من الأيام بالستيكوجاست + +544 +00:40:54,620 --> 00:40:57,560 +ووصف واضحة الصورة هذه أن الـ Antistryptol هو .. + +545 +00:40:57,560 --> 00:40:59,640 +واخدوا بالكم من أحد البروحانات المشهورة جدا في + +546 +00:40:59,640 --> 00:41:03,740 +قطاع غزة للتعارف على أن مسبب اللواز هو + +547 +00:41:03,740 --> 00:41:08,740 +Stryptococcus Bionica كمان شغل مهم جدا أنا قولت + +548 +00:41:08,740 --> 00:41:13,880 +فيه تايتر، صح؟ التايتر لما بنجيسه، احنا بنعطيه في + +549 +00:41:13,880 --> 00:41:18,300 +التقرير للطبيب، بنعطي التقرير، بنقول أن التايتر + +550 +00:41:18,300 --> 00:41:23,280 +800 مثلا، هو 200، 400، 800، 1600، اللي هو cereal + +551 +00:41:23,280 --> 00:41:27,780 +dilutionفكنا له 800 مثلا الطبيب هنا لازم ياخد باله + +552 +00:41:27,780 --> 00:41:31,360 +من انه ال concentration عالية والاسبوع الماضي + +553 +00:41:31,360 --> 00:41:37,220 +تذكروا تحدثنا عن بعض الأمراض المرتبطة بال immunity + +554 +00:41:37,220 --> 00:41:43,500 +بمعنى آخر ان الميكروبات بتنتج أنتجينات شبيهة + +555 +00:41:43,500 --> 00:41:48,040 +بأنتجناتناال antibodies اللي تنتج مش بس بتهاجم + +556 +00:41:48,040 --> 00:41:52,440 +البكتيريا بتهاجم برضه و من ضمن أسوأ الميكروفات + +557 +00:41:52,440 --> 00:41:55,820 +اللي هي ال streptococcus بيو جينز تمام؟ وبالتالي + +558 +00:41:55,820 --> 00:41:59,000 +لما بيلاقي title عالي يبقى هذا المرض بيكون under + +559 +00:41:59,000 --> 00:42:02,880 +observation طيلة فترة للعلاج حتى يمثل مع ال + +560 +00:42:02,880 --> 00:42:07,560 +complications اللي احنا تحدثنا عنها فحص مهم فحص + +561 +00:42:07,560 --> 00:42:11,060 +شائع عرفنا اليوم المصدر تبعه اللي هو ال + +562 +00:42:11,060 --> 00:42:15,630 +streptolysinواللي هو الخاص بمين بالـ streptococcus + +563 +00:42:15,630 --> 00:42:21,450 +biogenes طيب فينا اللي هو ال .. ال .. ال biogenic + +564 +00:42:21,450 --> 00:42:24,170 +exotoxins وهو عبارة عن erythrogenic كلمة + +565 +00:42:24,170 --> 00:42:26,450 +erythrogenic يعني واضح من انه .. انه بي .. بي .. + +566 +00:42:26,450 --> 00:42:30,210 +بيستهدف مين اللي هو بيستهدف ال .. ال red blood + +567 +00:42:30,210 --> 00:42:34,190 +cell ال hyaluronidase اللي قلنا بيفكفك الخلايا عن + +568 +00:42:34,190 --> 00:42:40,390 +بعضها البعض ال streptokinase ماروا معانا؟ kinases + +569 +00:42:40,390 --> 00:42:41,970 +عكس مين؟ + +570 +00:42:44,690 --> 00:42:47,830 +عكس الكواغوليس، عكس الكواغوليس، قولنا الكواغوليس + +571 +00:42:47,830 --> 00:42:52,370 +ده ما بيحول الفيبرونيجن إلى فيبرين، هذا بيحول + +572 +00:42:52,370 --> 00:42:55,750 +الفيبرونيجن أو بيبام ال lysis لفيبرونيجن، بيبام ال + +573 +00:42:55,750 --> 00:42:59,590 +lysis لعفوة لما الفيبرين نقلت، وبالتالي بعيق عملية + +574 +00:42:59,590 --> 00:43:02,650 +مين قولنا؟ عملية ال healing للجروح، يعني الجروح ما + +575 +00:43:02,650 --> 00:43:07,620 +بتشفى، ما بتتكون جلطةالان البكتيريا موجودة و بتنتج + +576 +00:43:07,620 --> 00:43:11,260 +streptokinase و قولنالكوا أصبح streptokinase هذا + +577 +00:43:11,260 --> 00:43:16,420 +بالظبط سلعة مهمة دوائية تستخدم في علاج الجذبات + +578 +00:43:16,420 --> 00:43:23,260 +للمرضى اللي بيصير عندهم اللي هو الجذبات في ال + +579 +00:43:23,260 --> 00:43:27,020 +streptodoranase وهو عبارة عن deoxyribonuclease + +580 +00:43:27,020 --> 00:43:29,980 +يعني على غرار اللي هو ال dianase تبع ال + +581 +00:43:29,980 --> 00:43:35,060 +staphylococcus streptodoranase اسمهوطبعا هذا برضه + +582 +00:43:35,060 --> 00:43:42,020 +له أهمية في عملية الحصول أو تكسير المادة + +583 +00:43:42,020 --> 00:43:44,820 +النووية وبتاع الحصول عليها القواعد كغذاء + +584 +00:43:44,820 --> 00:43:52,420 +لالبكتيريا ف kill in proteinالـ M-protein المفروض + +585 +00:43:52,420 --> 00:43:55,700 +تكون فاكرينه الأصناع من ضمن من ضمن ال virulence من + +586 +00:43:55,700 --> 00:44:00,820 +ضمن ال virulence factors ويقولنا عنه antifagocytic + +587 +00:44:00,820 --> 00:44:06,740 +antifagocytic يعني بيمنع الفاجو سيتوسز وبالتالي هو + +588 +00:44:06,740 --> 00:44:11,760 +وسيلة جيدة من قبل البكتيريا لي حماية نفسها من اللي + +589 +00:44:11,760 --> 00:44:16,240 +حماية الفاجو سيتوسز تذكروا بتكلم عن biogenic + +590 +00:44:16,240 --> 00:44:20,900 +infection biogenic infection يعني ال bus ايش ال + +591 +00:44:20,900 --> 00:44:21,040 +bus + +592 +00:44:26,430 --> 00:44:32,870 +النترو فيلز هي الفاجو سايتس فبالتالي بيعطي قدرة + +593 +00:44:32,870 --> 00:44:38,150 +للبكتيريا انه يقتل الفاجو سايتس مش الفاجو سايتس هي + +594 +00:44:38,150 --> 00:44:42,990 +تقتل البكتيريا لانه بيهرب من الفاجو سايتس طبعا + +595 +00:44:42,990 --> 00:44:47,810 +غالبا ال immunity اللي بتنتج انها بتكون نتجة ضد + +596 +00:44:47,810 --> 00:44:51,970 +مين ضد اللي هو ال protein M يعني لو واحد جسمه كوّن + +597 +00:44:51,970 --> 00:44:54,790 +أجسام مضطرة ل protein M تمام هي اللي بتعطيه + +598 +00:44:54,790 --> 00:44:59,450 +immunityبرضه هو مسئول عن ال strain variation يعني + +599 +00:44:59,450 --> 00:45:03,210 +البكتيريا ممكن تغير ال protein M تبعها وبالتالي + +600 +00:45:03,210 --> 00:45:09,370 +تدخل في protein M1 و بعدين M2 M3 M4 وبالتالي + +601 +00:45:09,370 --> 00:45:15,030 +بعطيها هي ما يسمى ب strain variation تنوع السلالات + +602 +00:45:15,030 --> 00:45:21,810 +فكل مرة بيدخل الميكروب ب strain جديد أنا عندي ل M1 + +603 +00:45:21,810 --> 00:45:27,850 +هو دخل ل M2 من ف M1 من ف M1 صح؟رقم تلاتة ال + +604 +00:45:27,850 --> 00:45:31,410 +reinfection هو المسئول عن ال reinfection مدام هو + +605 +00:45:31,410 --> 00:45:34,070 +اللي بيعمل strain variation المفروض ان انا وصاب + +606 +00:45:34,070 --> 00:45:38,070 +مرة واحدة و يتكوّل عندي مناعة تمام و انت هالموضوع + +607 +00:45:38,070 --> 00:45:42,630 +لكن انا وصاب مرة تلو المرة بسبب ورود M protein + +608 +00:45:42,630 --> 00:45:46,310 +لانه أخلف ال strains و هو بتاعي سهل عاملت بيه ال + +609 +00:45:46,310 --> 00:45:50,910 +reinfectionكابسول برضه في بعض ال .. ال .. ال .. + +610 +00:45:50,910 --> 00:45:53,950 +الستبتوكوكاية إلها كابسول و هي عبارة عن anti + +611 +00:45:53,950 --> 00:45:56,870 +-phagocytic غالبا ال strings اللي إلها كابسول + +612 +00:45:56,870 --> 00:46:01,670 +بتكون ميوكاين، من أشهر الستبتوكوكاية اللي إلها .. + +613 +00:46:01,670 --> 00:46:03,470 +من أشهر الستبتوكوكاية اللي إلها كابسول + +614 +00:46:10,920 --> 00:46:14,880 +الـ streptococci مجموعة ضخمة، ضخمة ضخمة كبيرة في + +615 +00:46:14,880 --> 00:46:18,860 +منها بناكلوها احنا مع اللبن، يعني بيستخدموها ل .. + +616 +00:46:18,860 --> 00:46:23,500 +اسمها streptococcus lactis streptococcus lactis + +617 +00:46:23,500 --> 00:46:27,280 +إنها بتعمل ال .. ال .. ال lactic acid من الحليب + +618 +00:46:27,280 --> 00:46:33,480 +ففي منها pathogenic وفي منها non pathogenic فتم + +619 +00:46:33,480 --> 00:46:37,540 +تصنيفها بطريقتين، طريقة ال homolysis فاكرينها؟ + +620 +00:46:37,540 --> 00:46:41,710 +Alpha, Beta, Gammaهو الطريقة التانية هال يلا + +621 +00:46:41,710 --> 00:46:45,090 +انبسطوا الآن العالمة اللي موجودة بكورة اسمها علمة + +622 +00:46:45,090 --> 00:46:49,850 +اسمها Rebecca Lansfield Rebecca Lansfield أنضت + +623 +00:46:49,850 --> 00:46:53,930 +حياتها في تصنيف لستريبتو كوكاي based on a C + +624 +00:46:53,930 --> 00:46:56,530 +-Polysaccharide موجود في ال cell wall تبع لستريبتو + +625 +00:46:56,530 --> 00:46:59,790 +كوكا يعني صنفت البكتيريا based on antigenic + +626 +00:46:59,790 --> 00:47:02,990 +structure C-Polysaccharide موجود في من ال cell + +627 +00:47:02,990 --> 00:47:08,870 +wall وهو بقى عن surface antigen الان + +628 +00:47:09,410 --> 00:47:12,530 +لما جينا نجمع كل ال microbiota اللى اسمها stirt + +629 +00:47:12,530 --> 00:47:17,150 +coccus اللى جينا انه فيه بينطبق عليها ال system + +630 +00:47:17,150 --> 00:47:20,990 +تبع Rebecca Lansfield وفيه بينطبقش عليها فاش صموها + +631 +00:47:20,990 --> 00:47:25,990 +groupable و non groupable groupable يعني .. يعني + +632 +00:47:25,990 --> 00:47:30,430 +يمكن تصنيفها بتصنيف Rebecca Lansfield و التانية + +633 +00:47:30,430 --> 00:47:34,550 +non groupable معناها تحكي انه لا تمتلكلاتمتلك الـ + +634 +00:47:34,550 --> 00:47:38,510 +Antigenات اللي بلد عليها Rebecca Lansfield اللي هو + +635 +00:47:38,510 --> 00:47:43,710 +تصنيفها حصل منها Non-Growable من أهم المجموعات A, + +636 +00:47:43,870 --> 00:47:47,930 +B و D الـ C و الـ G و ال F أقل الأهمية لكن اللي هم + +637 +00:47:47,930 --> 00:47:51,330 +الـ A و ال B و ال D هم اللي هندرسهم خلال المحاضرة + +638 +00:47:51,330 --> 00:47:54,210 +اللي جاية و هي بس بعد السؤال الأخرى في محاضرتنا + +639 +00:47:54,210 --> 00:47:57,450 +الـ Non-Growable تشمل واحد من الميكروبات المهم جدا + +640 +00:47:57,450 --> 00:48:01,850 +اللي هي Steptococcus pneumoniaو إلها شبيه مورود في + +641 +00:48:01,850 --> 00:48:06,050 +الفيلم بشكل كبير اسمه Stratococcus Mutans و بتسبب + +642 +00:48:06,050 --> 00:48:10,710 +Dental Chaos او تسوص اللي هو الاسم باكن كل حاجة + +643 +00:48:10,710 --> 00:48:12,970 +انهينا محاضرتنا و ان شاء الله نكمل مرة القاهرة + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vbNOwwvDE6k_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vbNOwwvDE6k_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..61e8720b35fc86171129cdf58da948f0dbaf8b77 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vbNOwwvDE6k_postprocess.srt @@ -0,0 +1,2424 @@ +1 +00:00:20,500 --> 00:00:23,820 +بسم الله الرحمن الرحيم الموضوع الجامعه هيكون عن + +2 +00:00:23,820 --> 00:00:27,660 +السيرمونيلا ان شاء الله وبينختم الجزء الأول من ال + +3 +00:00:27,660 --> 00:00:30,100 +gram negative بس الله هنتقل الجزء التاني اللي + +4 +00:00:30,100 --> 00:00:34,580 +متعلق بال Vibrio و ال pseudomonas السيرمونيلا + +5 +00:00:34,580 --> 00:00:39,940 +بتسبب تلت أشكال من الأمراض الصحية ممكن أن ألخصهم + +6 +00:00:39,940 --> 00:00:45,820 +في three categories رقم 1 gastroenteritis وهي تشبه + +7 +00:00:52,040 --> 00:00:54,900 +والنوع التاني اللي هو ال enteric fevers اللي هو + +8 +00:00:54,900 --> 00:00:58,660 +بينتمي له ال tayfoid و الفيرة tayfoid fever و + +9 +00:00:58,660 --> 00:01:02,820 +الشكل التالت بتلخص فيه septicemia و ال septicemia + +10 +00:01:02,820 --> 00:01:08,480 +هي زي اي gram negative سبتسيميا اخرى زي ما نقول + +11 +00:01:08,480 --> 00:01:11,980 +مثلا لزوجة وراس ايه الجنوزة سبتسيميا ايه شيريشيا + +12 +00:01:11,980 --> 00:01:16,000 +كولاعي سبتسيمياطبعا هذا لا يقلل من الخطوة لأن في + +13 +00:01:16,000 --> 00:01:19,520 +الآخر ال gram negative septicemia كل كُبتها مافيش + +14 +00:01:19,520 --> 00:01:24,780 +مضارة ان ال endotoxin لما سيل ريليز اما من خلال + +15 +00:01:24,780 --> 00:01:29,020 +قتل الجسم للبكتيريا او من استخدام ال antibiotics + +16 +00:01:29,020 --> 00:01:32,640 +لقتل البكتيريا بينتج عنها ما يسمى ال gram negative + +17 +00:01:32,640 --> 00:01:37,520 +septic shock لكن هاي الأثار الأشكال من disease + +18 +00:01:37,520 --> 00:01:39,680 +كمان مرة gastroenteritis + +19 +00:01:47,910 --> 00:01:51,450 +السيرمونيلة زيها زي الانتروبكترياسي فيها O أنتجين + +20 +00:01:51,450 --> 00:01:59,670 +وكونها موطعي فيها H أنتجين والبعض منها بيحتوي على + +21 +00:01:59,670 --> 00:02:07,050 +capsule ال capsule هنا تسمى V-I أنتجين + +22 +00:02:10,040 --> 00:02:13,920 +هو الـ A-antigen ما عادة قلناه السبب هو انه بيتلخص + +23 +00:02:13,920 --> 00:02:16,580 +في 3 في 3 أنواع من الانتجينات اللي هم ال O-antigen + +24 +00:02:16,580 --> 00:02:19,660 +وهو common لكل ال bacterium و لكل الجراح نيجاتي و + +25 +00:02:19,660 --> 00:02:24,180 +ال H-antigen فقط لتلك المتحركة منها و ال I-antigen + +26 +00:02:24,180 --> 00:02:28,240 +كمان اللي هو ال V-I-antigen لتلك التي تمتلك اللي + +27 +00:02:28,240 --> 00:02:32,630 +هو ال capsuleطب انا قولت لتلك المتحركة منها، هل + +28 +00:02:32,630 --> 00:02:36,110 +هناك أنواع من السلمونيلا غير متحركة؟ طب احنا كل ما + +29 +00:02:36,110 --> 00:02:40,090 +بنقول انه السلمونيلا بنميزها عن الشيجلة بال + +30 +00:02:40,090 --> 00:02:43,870 +motility، بقى معناه ال motile سلمونيلا، non motile + +31 +00:02:43,870 --> 00:02:48,250 +شيجلة، هل هناك non motile سلمونيلا؟ نعم هناك two + +32 +00:02:48,250 --> 00:02:50,810 +species of non motile، يعني دايما في exceptions، + +33 +00:02:50,810 --> 00:02:55,850 +دايما في exceptionsالحدث هنا بيقول انه ال .. ال .. + +34 +00:02:55,850 --> 00:02:59,090 +ال serotyping او التعرف الدقيق على السيمونيلا + +35 +00:02:59,090 --> 00:03:03,290 +لايتم بشكل روتيني في المختبرات نظرا لإنه بيتكلم عن + +36 +00:03:03,290 --> 00:03:06,250 +two thousand serotypes وبتالي بيتم في ما يسمى ب + +37 +00:03:06,250 --> 00:03:10,560 +reference laboratories يعني مختبرات مرجعيةمختبرات + +38 +00:03:10,560 --> 00:03:15,640 +مرجعية بيكون فيها monovalent antiserum لكل سيرو + +39 +00:03:15,640 --> 00:03:19,860 +طاعد من المردين بس احنا ممكن نخزن ما يسمى بال + +40 +00:03:19,860 --> 00:03:23,740 +polyvalent و ال polyvalent salmonella antiserum + +41 +00:03:23,740 --> 00:03:28,600 +الغلط منها مش تحديد هوية السلمونيلا بقدر ما هو + +42 +00:03:28,600 --> 00:03:32,080 +معرفة ما إذا كان الميكروب أصلا سلمونيلا ولا مش + +43 +00:03:32,080 --> 00:03:34,760 +سلمونيلا يعني تعريف عام للسلمونيلا + +44 +00:03:36,990 --> 00:03:40,150 +طبعا ال salmonella مرد بتصنيفات عديدة مش هنقود + +45 +00:03:40,150 --> 00:03:42,750 +فيها ال .. في هذا الفصل هنقود فيها الفصل القادم ان + +46 +00:03:42,750 --> 00:03:45,830 +شاء الله نتكلم عن ال salmonella بشكل مفصل أكتر لكن + +47 +00:03:45,830 --> 00:03:49,770 +genetically معظم .. معظم أنواع ال salmonella تنتمي + +48 +00:03:49,770 --> 00:03:53,430 +إلى one species على الرغم من أسماء العديدة + +49 +00:03:53,430 --> 00:03:57,030 +المتنوعة في ال salmonella إلا أنه فينا one species + +50 +00:03:57,030 --> 00:04:02,690 +اسمها salmonella entericمثلا من السلمونيلا + +51 +00:04:02,690 --> 00:04:05,950 +انتليكيا بنلاقي في أصناف سلمونيلا انترادايدس ومن + +52 +00:04:05,950 --> 00:04:10,810 +الإسم انترادايدس تمال انترايدس أو gastroenteritis + +53 +00:04:10,810 --> 00:04:15,590 +يعني إسهال ومنها many serotypes في سلمونيلا كوليرة + +54 +00:04:15,590 --> 00:04:19,350 +سويس والكلرة سويس سويس معناها أكلامك الله خنزير + +55 +00:04:19,350 --> 00:04:24,140 +كوليرة بتعرف الكوليرةفكانت هذه البكتيريا عُزلة من + +56 +00:04:24,140 --> 00:04:28,140 +condition سمية أو اصطفت تفق الاصطلاح عن انه + +57 +00:04:28,140 --> 00:04:31,220 +cholera الخنازير، cholera الخنازير، لما عزلوا + +58 +00:04:31,220 --> 00:04:34,640 +الميكروم لجوا انه Salmonella Fasamua، Salmonella + +59 +00:04:34,640 --> 00:04:37,560 +cholera Fasamua، لكن هذه البكتيريا بتعملنا + +60 +00:04:37,560 --> 00:04:41,790 +septicemia يعني، بتعملنا septicemiaالسلمونيلا + +61 +00:04:41,790 --> 00:04:44,530 +طايفي اللى بتسيب طبعا الtyphoid فى سلمونيلا بارا + +62 +00:04:44,530 --> 00:04:47,770 +طايفي والبارا طايفي نفسها بتكون a,b ولا c يعنى فى + +63 +00:04:47,770 --> 00:04:53,230 +أسماء كثيرة لكن فى الآخر تتصنف they all belong to + +64 +00:04:53,230 --> 00:04:59,730 +one species اللى سلمونيلا انتلج سلمونيلا انتلج طيب + +65 +00:05:00,560 --> 00:05:04,420 +الشجلة أذكركوا بجملة من الجملة اللي دراسناها man + +66 +00:05:04,420 --> 00:05:10,960 +is the only non-natural host for شجلة واتفقنا لو + +67 +00:05:10,960 --> 00:05:15,200 +قدرنا بطريقة ما نصل لكل البشر المصابين بالشجلة + +68 +00:05:15,200 --> 00:05:19,740 +وعالجناهم نتخلص من الشجلة إلى الأبد لكن السينامولة + +69 +00:05:19,740 --> 00:05:25,180 +الحال مختلف طبعا كمان مرة ال speciesSpecific بمعنى + +70 +00:05:25,180 --> 00:05:29,260 +ال salmonella تايفي تشبث الشجلة بهذا الحصوص man is + +71 +00:05:29,260 --> 00:05:33,740 +the only known host لكن ال other salmonella للأسف + +72 +00:05:33,740 --> 00:05:38,540 +استطاعت أن تجد آلاف الأنواع من ال reservoirs وأنا + +73 +00:05:38,540 --> 00:05:44,040 +ممكن أحكي تلكوا قبل هيك السلاحف التماسيح الزواحف + +74 +00:05:44,040 --> 00:05:47,780 +اللي من اللي جيها من البيوت الدواجن مثل الدجاج + +75 +00:05:47,780 --> 00:05:50,820 +والبط والأوز والأشياء اللي بتتعامل معاها كغداء + +76 +00:05:50,820 --> 00:05:55,290 +تمام بتربيها كغداءحتى البيض قلنا قد يحتوي على + +77 +00:05:55,290 --> 00:05:59,470 +سالمونيلا فلو فكرنا نتخلص من السالمونيلا العملية + +78 +00:05:59,470 --> 00:06:03,170 +هتكون mission impossible يعني مهمة مستحيلة مهمة + +79 +00:06:03,170 --> 00:06:06,750 +شبه مستحيلة لإنه في reservoir كتيرة منها بنعرفه + +80 +00:06:06,750 --> 00:06:12,780 +ومنها المازلنا بنكتشف باستمرار reservoir جديدمن ال + +81 +00:06:12,780 --> 00:06:17,940 +violence factors اللي بتأهل السلمونيلا تكون هالاسم + +82 +00:06:17,940 --> 00:06:21,920 +اللامع اللي انا اضطريت اقولكه جملة مرة و بدا + +83 +00:06:21,920 --> 00:06:25,260 +اعيدلكيها مرة تانية the presence of any zero type + +84 +00:06:25,260 --> 00:06:28,440 +of salmonella in food rendered that food unfit for + +85 +00:06:28,440 --> 00:06:33,830 +human consumptionمؤشر على أن هذه الجملة بحيث ذاتها + +86 +00:06:33,830 --> 00:06:38,930 +هي تشريعات وقوانين في كل دولة العالم، أي إذا بيصنع + +87 +00:06:38,930 --> 00:06:42,830 +أو بيصنع جمهور يجب أن يكون خالي من مين؟ من السمنة، + +88 +00:06:42,830 --> 00:06:48,630 +تخيلوا التابعات الملقاة على هذا القرارالمصانع إيش + +89 +00:06:48,630 --> 00:06:51,630 +بديها تعمل؟ المزارع اللي بتودّيها للمصانع إيش + +90 +00:06:51,630 --> 00:06:54,950 +بديها تعمل؟ المزارع و الخضروات تبعها إيش بديها + +91 +00:06:54,950 --> 00:06:58,590 +تعمل؟ ممنوع يستخدم مخلفات دواجن فيها سلمونيلا في + +92 +00:06:58,590 --> 00:07:02,390 +التسمين، المصنع لازم يعطينا دواجن خالي من مين من + +93 +00:07:02,390 --> 00:07:09,250 +السلمونيلا، يبقى العملية هتكون إرهاق لكل العالم، + +94 +00:07:09,250 --> 00:07:14,010 +حتى يعطونا منتج خالي من مين من اسمهتماني هي + +95 +00:07:14,010 --> 00:07:19,890 +السلمونيلا التي أرقط العالم كله عندها اندوكسين + +96 +00:07:19,890 --> 00:07:22,910 +زيها زي أي gram negative بسلاي وانتوا عارفين انه + +97 +00:07:22,910 --> 00:07:27,570 +necessary for survival البكتيريا كابسول البعض زي + +98 +00:07:27,570 --> 00:07:34,130 +ال type and some paratype اللي شبه بالtype عندها + +99 +00:07:34,130 --> 00:07:39,330 +أدهسين منها ما هو متعلق بالفمبريا ومنها ما هو غير + +100 +00:07:39,330 --> 00:07:40,330 +متعلق بالفمبريا + +101 +00:07:48,680 --> 00:07:52,940 +النوع الأول اللي يضحك على البكتيريا ويقولها يعني + +102 +00:07:52,940 --> 00:07:58,800 +يقرأ phagocyte يعني take me in النوع التاني بيخلي + +103 +00:07:58,800 --> 00:08:04,760 +الفاجوزوم مايرتبطش بالليزوزوم يبقى فيندي نوعين من + +104 +00:08:04,760 --> 00:08:08,200 +ال type 3 secretion system على فكرة في type 6 + +105 +00:08:08,200 --> 00:08:14,600 +secretion system ابتشرفوها حديثةبشبهو بما يسمى + +106 +00:08:14,600 --> 00:08:19,440 +الخنجر المسبوم اللي loaded with spring اللي مضغوط + +107 +00:08:19,440 --> 00:08:23,840 +بزنبرك، زنبرك لو ضغطنا بفلت بطير، تعرفوا في + +108 +00:08:23,840 --> 00:08:29,270 +أفريقيا في صيد بسهم انبوببكون طويل و في طرفه فيه + +109 +00:08:29,270 --> 00:08:33,010 +سهم، امام؟ السهم بكون معمور على شكل قمة من ورا، + +110 +00:08:33,010 --> 00:08:36,490 +فلو بننفخ، بننفخ في القمة احنا، فبيعمل blow و .. + +111 +00:08:36,490 --> 00:08:40,610 +فبينطلق مسافة لبسها بيها و بصيب الآخرين البكتيريا + +112 +00:08:40,610 --> 00:08:44,090 +عندها system type VI, secretion system يشبه هذا + +113 +00:08:44,090 --> 00:08:49,690 +النوع، بينضغط و بينطلق مرة واحدة عند الملانسة، + +114 +00:08:49,690 --> 00:08:55,260 +ملامسة بكتيريا أخرى أو ملامسة host cellفالواضح ان + +115 +00:08:55,260 --> 00:08:58,440 +احنا مابنعرفش كل إيش عن البكتيريا لسه بنتعلم و + +116 +00:08:58,440 --> 00:09:01,120 +جديد و اننا اكتشفنا اللي هو ال type 6 secretion + +117 +00:09:01,120 --> 00:09:05,340 +system اللي حاكينا عنه طيب من المميزات ايضا في ان + +118 +00:09:05,340 --> 00:09:09,160 +حاسمة outer membrane proteins او ال OMB وهذه + +119 +00:09:09,160 --> 00:09:11,760 +involved in the ability of semella to survive + +120 +00:09:11,760 --> 00:09:15,460 +inside macrophages يعني إيه علاقة بمخزن البكتيريا + +121 +00:09:15,460 --> 00:09:19,480 +في بقاء الماكروفاج الفلاجلة أو كل كبتارة فوق انه + +122 +00:09:19,480 --> 00:09:23,600 +الفلاجلةOrgan of motility بيساعد البكتيريا الى + +123 +00:09:23,600 --> 00:09:29,020 +ادهاب الى طعام او الهروب من خطر وهذا يعني تخيلوا + +124 +00:09:29,020 --> 00:09:33,520 +لو بدنا نقول invasive بدنا نقول invasive ما معناه + +125 +00:09:33,520 --> 00:09:38,160 +invasive غزو الغزو في انتقال من مكان الى مكان مين + +126 +00:09:38,160 --> 00:09:42,300 +اسهل ان يعمل invasion متحرك ولا غير متحرك اكيد + +127 +00:09:42,300 --> 00:09:46,700 +متحرك مع العلم ان غير متحرك ايضا بيعمل invasion زى + +128 +00:09:46,700 --> 00:09:51,020 +مثلا قلنالكوا في بكتير تستغلال .. ال .. الاكتين + +129 +00:09:51,020 --> 00:09:54,160 +وبتتحرك من .. بالظبط بتتحرك من خلال اللي هو ال .. + +130 +00:09:54,160 --> 00:09:58,780 +الاكتين والبكتيريا غريبة في أطوارها وفي قدراتها + +131 +00:09:58,780 --> 00:10:02,700 +وفي قدرتها على استغلال الموارد اللي موجودة يعني في + +132 +00:10:02,700 --> 00:10:07,400 +.. يعني مثلا بكتيريا زي مثلا بكتيريا الطاوم معروف + +133 +00:10:07,400 --> 00:10:11,180 +عنها بتنتقلش إلا من خلال .. إلا من خلال حشرات اللي + +134 +00:10:11,180 --> 00:10:17,200 +هي البراغين تبعة الأرس الجردان، تمام؟لكن خلال + +135 +00:10:17,200 --> 00:10:23,000 +مرورها في الدسم لو وصلت لل lungs بتقدر تكتسب صفات + +136 +00:10:23,000 --> 00:10:26,640 +معينة نوية في ال lungs اتاهلها تنتقل من إنسان إلى + +137 +00:10:26,640 --> 00:10:30,920 +إنسان دون المرور بالحشرة .. دون المرور بالحشرة .. + +138 +00:10:30,920 --> 00:10:35,300 +إذا المفهوم أو ال concept إن البكتيريا تستطيع أن + +139 +00:10:35,300 --> 00:10:39,940 +تستغل الموارد وتتغير بتغير البيئة الموجودة حولها + +140 +00:10:39,940 --> 00:10:45,320 +.. كمان مثالال bacillus anthracis عُزِلت من المرضى + +141 +00:10:45,320 --> 00:10:51,020 +دائما لها كابسول لما بنزرعها على بلايز تطلع بدون + +142 +00:10:51,020 --> 00:10:56,180 +كابسول لو ضفنا على بلايز calcium carbonate بطلع + +143 +00:10:56,180 --> 00:11:01,740 +لها كابسول يبقى بعض الأحيان صفات البكتيريا بتتأثر + +144 +00:11:01,740 --> 00:11:06,960 +بالغذاء أو بال environment المحيطة بيه طيب + +145 +00:11:08,110 --> 00:11:11,250 +الانتروتوكسين والانتروتوكسين لنفهم أنه هذه بكتيريا + +146 +00:11:11,250 --> 00:11:13,550 +.. هذه بكتيريا السمنلة .. بتكلم عن السمنلة بشكل + +147 +00:11:13,550 --> 00:11:17,670 +عام، عن الطائفة و البراطايفة و الاندروديلس و الكل، + +148 +00:11:17,670 --> 00:11:22,270 +أن البعض منها فقط بيمتلك الاندو .. الاندوتوكسين، + +149 +00:11:22,270 --> 00:11:25,530 +بس البعض الآخر بيمتلك برضه إكزوتوكسين من صفاته أنه + +150 +00:11:25,530 --> 00:11:31,110 +انتروتوكسينفي ال midterm جينا سؤال جينا فرق بين ال + +151 +00:11:31,110 --> 00:11:34,190 +endotoxin و ال exotoxin طبعا هذه السؤال اعتقد انه + +152 +00:11:34,190 --> 00:11:37,390 +اللي ماجاوبش عليه يعني بده يقعد معانا السنة اللي + +153 +00:11:37,390 --> 00:11:39,230 +جاينه و يقعد معانا السنة اللي جاينه ان شاء الله + +154 +00:11:39,230 --> 00:11:43,450 +حارب يعنى لأنه very simple و أحفظناه احنا في + +155 +00:11:43,450 --> 00:11:50,290 +المحاضرة جون ال endotoxin is exclusive لل gram + +156 +00:11:50,290 --> 00:11:54,520 +negative بس ال exotoxin مش exclusive لحدهابتنتجوا + +157 +00:11:54,520 --> 00:11:57,220 +الـ Gram Negative وبتنتجوا ال Gram Positive ومن + +158 +00:11:57,220 --> 00:11:59,820 +ضمن ال Gram Negative بتنتجوا اللي هي ال Salmonella + +159 +00:11:59,820 --> 00:12:03,560 +لما نصبح من الصفات المهمة The ability of the + +160 +00:12:03,560 --> 00:12:07,520 +Salmonella to capture Iron عند المقدر على التقاط + +161 +00:12:07,520 --> 00:12:11,040 +ال Iron وهذا نقول عنها من ال Virulence Factor اللي + +162 +00:12:11,040 --> 00:12:14,020 +فيها نوع من ال competition بينها وبين ال host لأن + +163 +00:12:14,020 --> 00:12:16,880 +ال host بده ال Iron وانا بده ال Iron مين بياخده + +164 +00:12:16,880 --> 00:12:20,200 +Iron أكتر هو اللي بيكون مسيطر على ال .. ال .. + +165 +00:12:20,200 --> 00:12:26,530 +الوضع في ال .. في ال infectionالانتليك أوتيفايتفر + +166 +00:12:26,530 --> 00:12:30,270 +ناخد فكرة عنه يالا نركز معايا أنا بلاش الصبورة + +167 +00:12:30,270 --> 00:12:35,190 +المرة هادى باختصار شديد جدا ماء ملوث ماء ملوث + +168 +00:12:35,190 --> 00:12:40,770 +شربناه فيه سالمونيلا السالمونيلا جزء منها بيموت في + +169 +00:12:40,770 --> 00:12:44,530 +ال .. في ال .. في ال .. في الستمك لإن ال harsh + +170 +00:12:44,530 --> 00:12:49,010 +environment في الستمك من low pH قادر على قتل كثير + +171 +00:12:49,010 --> 00:12:52,790 +من الميكروبات ومن ضمنها السالمونيلالان احنا نشرب + +172 +00:12:52,790 --> 00:12:55,190 +ميه كتير او غداء كتير غالبا اش اللي بيصير عندنا في + +173 +00:12:55,190 --> 00:12:58,630 +المعيدة بيصير لها dilution وجود غداء كمان بيعمل + +174 +00:12:58,630 --> 00:13:03,770 +protection لمين؟ لالميكرو Organism توجود غداء في + +175 +00:13:03,770 --> 00:13:06,910 +المعيدة بيعمل protection البعض منها بيمر سهل ما + +176 +00:13:06,910 --> 00:13:10,510 +الى ال intestinal tract بيصي ال intestinal tract + +177 +00:13:10,510 --> 00:13:14,950 +قصة من الله بتقول لبعضها او لحالها بتقول هذا + +178 +00:13:14,950 --> 00:13:19,600 +المكان ليس مناسبا للمعيشة لكنخلّيكوا شاب انتوا هنا + +179 +00:13:19,600 --> 00:13:23,020 +و انا و فلان و فلان بيقدروا نطلع نستكشف الوضع + +180 +00:13:23,020 --> 00:13:27,220 +اخترقوا جدار الأمعاة تروحوا على ال lymph node ال + +181 +00:13:27,220 --> 00:13:30,240 +lymph node احنا متفقين فيها macrophage الماكروفاج + +182 +00:13:30,240 --> 00:13:33,960 +المفروض وظيفته تقتل من البكتيريا لكن طول وجهها + +183 +00:13:33,960 --> 00:13:37,640 +بيبقوا السنغلة تلمقل على intracellular survival + +184 +00:13:37,640 --> 00:13:43,640 +فوين في ماكروفاج تحتك بالماكروفاج عشان هي تاكلها و + +185 +00:13:43,640 --> 00:13:47,400 +تتكثر جوها لإن ال ماكروفاج كمان بتوفرنا وسيلة نقلة + +186 +00:13:48,020 --> 00:13:51,660 +من مكان إلى مكان بسؤولة بعيدا عن جهاز المناع الآخر + +187 +00:13:51,660 --> 00:13:58,080 +من Antibodies وخلافه الآن ال macrophage تقمت + +188 +00:13:58,080 --> 00:14:01,040 +البكتيريا لكن البكتيريا تكاترت جوا ال macrophage + +189 +00:14:01,040 --> 00:14:05,230 +فال lymph node وظيفتها ألغيت تمام؟البكتيريا وصلت + +190 +00:14:05,230 --> 00:14:08,730 +في الدم لأن رحلة البكتيريا مبسوطة البكتيريا ال + +191 +00:14:08,730 --> 00:14:12,690 +salmonella بتجه دفقات من القلب دفقات بتعرفين كيف + +192 +00:14:12,690 --> 00:14:17,490 +القلب بيضخ الدم فال salmonella بتتمرجح معاه مع ال + +193 +00:14:17,490 --> 00:14:22,670 +circulation و قد تصل إلى أماكن مختلفة و متنوعة في + +194 +00:14:22,670 --> 00:14:26,810 +الدسم لكن هي لها أماكن مفضلة اللي هم ال + +195 +00:14:26,810 --> 00:14:29,910 +reticuloendothelial system زي ال spleen زي ال bone + +196 +00:14:29,910 --> 00:14:33,450 +marrow زي اللي قدر تحب تفضل .. بتفضل أماكنها دي + +197 +00:14:33,450 --> 00:14:38,340 +ليش؟لأنه فيها macro phage فيها macro phages و هي + +198 +00:14:38,340 --> 00:14:41,660 +قلنا بتدور على ال macro phage عشان تاكلها و تتكوّن + +199 +00:14:41,660 --> 00:14:47,190 +عضريا جواالان احنا اتأكدنا ان البكتيريا كانت + +200 +00:14:47,190 --> 00:14:50,870 +مولودة في الانتستين و ذهبت الى الدم و بعض ال + +201 +00:14:50,870 --> 00:14:54,650 +organs الأخرى و من ثم اختفت من الدم بظهور مين؟ + +202 +00:14:54,650 --> 00:14:58,250 +بظهور ال antibodies لكن ما زالت مولودة في ال + +203 +00:14:58,250 --> 00:15:00,370 +organs و ما زالت مولودة في ال intestine او + +204 +00:15:00,370 --> 00:15:03,430 +الintestine ليش الكلمة ده مهم؟ لنقعد في الجثة لما + +205 +00:15:03,430 --> 00:15:06,810 +نيجي نعمل diagnosis أفضل diagnosis هو blood + +206 +00:15:06,810 --> 00:15:10,350 +culture إذا بنعمل early diagnosis هو blood culture + +207 +00:15:11,000 --> 00:15:14,340 +و إذا بدنا نعمل follow-up لل treatment إنه نشوف + +208 +00:15:14,340 --> 00:15:17,920 +مثلا عندنا شخصنا المريض و عرفنا إنه عنده + +209 +00:15:17,920 --> 00:15:23,460 +salmonella type و أعطينا drug of choice الآن بعد + +210 +00:15:23,460 --> 00:15:28,480 +انتهاء الفترة العلاجية بدنا نتأكد إنه ما ضايل + +211 +00:15:28,480 --> 00:15:32,680 +بكتيريابناخدش عينة دم مرة أخرى، بناخد عينة stool، + +212 +00:15:32,680 --> 00:15:37,500 +بناخد عينة stool إذا لل diagnosis blood culture و + +213 +00:15:37,500 --> 00:15:40,700 +لل follow-up لل treatment بناخد إيه؟ stool culture + +214 +00:15:40,700 --> 00:15:44,640 +ممنوع نوقف علاج للمريض إلا لما يكون ال stool + +215 +00:15:44,640 --> 00:15:48,300 +culture بتبعته إيه؟ negative ليش؟ بدناش يتحول ل + +216 +00:15:48,300 --> 00:15:52,200 +career فاكرين ت avoid marry؟ بدناش نرجع و نكوّن ت + +217 +00:15:52,200 --> 00:15:55,880 +avoid marry أخرين في المجتمع فودنا نعالج المريض و + +218 +00:15:55,880 --> 00:16:00,680 +ما نحوله لإيه؟ ل .. اللي هو career مرة أخرىمن أهم + +219 +00:16:00,680 --> 00:16:03,260 +العوامل اللي بتقدي لانتشار السلمونية اللي هو ال + +220 +00:16:03,260 --> 00:16:06,840 +poor sanitary conditions وال poor sanitary + +221 +00:16:06,840 --> 00:16:11,760 +conditions معناها مستوى نظافة عامة ضايق خدوا + +222 +00:16:11,760 --> 00:16:15,130 +بالكوافي حالة اسمها personal hygiene و في حالة + +223 +00:16:15,130 --> 00:16:17,990 +اسمها poor sanitation ال personal hygiene هو + +224 +00:16:17,990 --> 00:16:22,550 +النظافة الشخصية قد نكون نحن نظيفين شخصيا تمام لكن + +225 +00:16:22,550 --> 00:16:25,410 +ال infrastructures اللي حوالينا زي ما يطلع بعض + +226 +00:16:25,410 --> 00:16:29,550 +الحيوانات في الشوارع بلاج القمامة او كده القطط + +227 +00:16:29,550 --> 00:16:32,430 +اللي لعب فيها الكلاب اللي لعب فيها مادة الحاجة ان + +228 +00:16:32,430 --> 00:16:36,310 +انا مستوى النظافة الشخصية اندي كويس لكن مستوى ال + +229 +00:16:36,310 --> 00:16:39,450 +sanitation condition في البلد بيكون poor و هذا + +230 +00:16:39,450 --> 00:16:44,320 +بيقدى الى انه يتلوث ال ..المياه هو بالتالي إمكانية + +231 +00:16:44,320 --> 00:16:49,820 +الانتقال بتكون عالية جدا ال Typhoid Lasmotherapy + +232 +00:16:49,820 --> 00:16:53,200 +ماينفعش إنه نسيبه و نقول هو self-limiting لا هو مش + +233 +00:16:53,200 --> 00:16:58,200 +self-limited لازم نعطيه antibioticsو ال + +234 +00:16:58,200 --> 00:17:00,740 +Antibiotics اللي بيكتبوها هنا جملة essential يعني + +235 +00:17:00,740 --> 00:17:04,660 +ضروري و ضروري بانجوسين أنه نعمل sensitivity لل + +236 +00:17:04,660 --> 00:17:08,680 +isolate بمعنى أن السلمونيلا أيضا من الميكروبات + +237 +00:17:08,680 --> 00:17:12,560 +اللي عندها المقرا تكون resistance الفاكسين + +238 +00:17:12,560 --> 00:17:15,760 +بيستخدموا نوعين من الفاكسين في نوع مش مذكور قدامنا + +239 +00:17:15,760 --> 00:17:18,640 +على الصبورة اللي هو life attenuated برضه بكتيريا + +240 +00:17:18,640 --> 00:17:24,180 +مضعفةاو .. او killed و بيستخدموا ايضا هو ال V.I. + +241 +00:17:24,460 --> 00:17:29,360 +كابسولات antigen ك vaccine و هو protective ايش + +242 +00:17:29,360 --> 00:17:34,480 +يعني protective؟ يعني بيحمي .. لإنه ال capsule أحد + +243 +00:17:34,480 --> 00:17:39,540 +أهم ال virulence factor و بالتالي ممكن تعمل + +244 +00:17:39,540 --> 00:17:45,260 +protection ل .. ل .. ل .. المرضالنوع التاني أو + +245 +00:17:45,260 --> 00:17:47,340 +الشكل التاني من السلمونولاز اللي هو ال + +246 +00:17:47,340 --> 00:17:50,420 +gastroenteritis و اللي بتسببه غالبا بقولنا + +247 +00:17:50,420 --> 00:17:56,760 +انترادايدس سلمونولا انترادايدس نبدأ من تحت self + +248 +00:17:56,760 --> 00:18:01,390 +limitingيعني ايش self-limiting؟ زي ال .. زي ال + +249 +00:18:01,390 --> 00:18:06,170 +influenza زي الرشح ما هو self-limiting بي .. بيصل + +250 +00:18:06,170 --> 00:18:10,070 +ال peak و بعدين بينزل لحاله self-limiting بيطيب + +251 +00:18:10,070 --> 00:18:13,810 +لحاله هاي condition ب bacteria ال infection + +252 +00:18:13,810 --> 00:18:18,790 +wherein antibiotics may be unnecessary يعني حتى لو + +253 +00:18:18,790 --> 00:18:21,770 +شخصنا لحالة مش ضروري نعالجها بال antibiotics لكن + +254 +00:18:21,770 --> 00:18:26,050 +نعالج الشقين اللي فاكرينهم بنوعهم electrolyte و + +255 +00:18:26,050 --> 00:18:30,830 +rehydrationإن نرجعله الأملح و نرجعله الملح، ده مش + +256 +00:18:30,830 --> 00:18:33,390 +داعي أنه .. مين اللي .. عن مين .. عن طريق مين + +257 +00:18:33,390 --> 00:18:39,790 +بينتقل؟ poultry, eggs و no human reservoir يعني مش + +258 +00:18:39,790 --> 00:18:42,810 +من .. مش من البناء الدمين و أنما من وين من ال + +259 +00:18:42,810 --> 00:18:45,290 +poultry و ال eggs، هدول ال most common هدول ال + +260 +00:18:45,290 --> 00:18:48,390 +most .. إيه بقى poultry؟ poultry معناها الدواجن، + +261 +00:18:48,390 --> 00:18:51,550 +دواجن بكل أشكالة مش الدجاج بس، البط و الوز، أي + +262 +00:18:51,550 --> 00:18:54,230 +حاجة بتأكل من الطيور، حمام حتى ممكن يستخدموا في + +263 +00:18:54,230 --> 00:18:55,650 +هذا ال .. في هذا الإطار + +264 +00:18:59,870 --> 00:19:03,490 +ال symptoms may include nausea, vomiting, diarrhea + +265 +00:19:03,490 --> 00:19:06,370 +و ال diarrhea بتكون non bloody يعني مابيش فيها + +266 +00:19:06,370 --> 00:19:09,890 +براز مدمم ال salmonella enteritis is the most + +267 +00:19:09,890 --> 00:19:13,510 +common isolate أكتر عذر نازلها من حالات salmonella + +268 +00:19:13,510 --> 00:19:18,970 +gastroenteritis هي salmonella enteritis و بيتميزوا + +269 +00:19:18,970 --> 00:19:21,770 +أنا قولت salmonella enterica salmonella enterica + +270 +00:19:21,770 --> 00:19:27,060 +غير salmonella enteritisال incubation period قد + +271 +00:19:27,060 --> 00:19:31,460 +تكون as short as 8 to 48 hours خدوا بالك و هذا + +272 +00:19:31,460 --> 00:19:35,520 +food infection ولا food poisoning؟ food infection + +273 +00:19:35,520 --> 00:19:40,780 +لأن هنا الميكروب يتكاتر جوا و بيعمل ال disease في + +274 +00:19:40,780 --> 00:19:43,480 +الداخل غير عن ال Staphylococcus اللي احنا خدنا + +275 +00:19:43,480 --> 00:19:47,700 +التكسين برا لكن mostly ال incubation period ممكن + +276 +00:19:47,700 --> 00:19:54,060 +تتراوح من ايه؟ من اتنين لخمس أيام اذا الأعراض تبدأ + +277 +00:19:54,060 --> 00:19:58,980 +فترةو ديزا بعد تناول الطعام أو الشراب الملوث بتمان + +278 +00:19:58,980 --> 00:20:00,520 +إلى أربعين واربعين ساعة + +279 +00:20:03,050 --> 00:20:06,650 +الشكل التالت من ال salmonellosis اللى هو ال + +280 +00:20:06,650 --> 00:20:10,350 +septicemia و اللى قولنا اكتر مايكروب بيعزل منه + +281 +00:20:10,350 --> 00:20:14,330 +اللى هو salmonella cholera suis لكن it could be + +282 +00:20:14,330 --> 00:20:17,110 +caused by any type of salmonella يعني كتير من ال + +283 +00:20:17,110 --> 00:20:22,010 +salmonella ممكن تسبب septicemia لكن ال cholera + +284 +00:20:22,010 --> 00:20:26,370 +suis هي أشهر عزلة نازلها من ال septicemiaو انا + +285 +00:20:26,370 --> 00:20:29,250 +كمان مرة بأكد على انه كلمة six email تعني انه + +286 +00:20:29,250 --> 00:20:33,170 +المرض سهل بالعكس خطير جدا وحتى في علاجه يجب أن + +287 +00:20:33,170 --> 00:20:36,510 +نكون حذرين جدا و يكون المريض under observation + +288 +00:20:36,510 --> 00:20:42,040 +خشية اللي هو ال ground negative septic shockأه في + +289 +00:20:42,040 --> 00:20:45,780 +بسمة مجموعة مرحوظة هنا أن ال disease is + +290 +00:20:45,780 --> 00:20:49,020 +characterized by limited fever يعني في حمّة متقطة + +291 +00:20:49,020 --> 00:20:52,640 +و no gastrointestinal involvement أو of little + +292 +00:20:52,640 --> 00:20:55,560 +يعني يا إما مافيش gastrointestinal involvement + +293 +00:20:55,560 --> 00:20:57,980 +مافيش مغلطس ولا اسئلة ولا أي أعراض + +294 +00:20:57,980 --> 00:21:01,380 +gastrointestinal أو بتكون قليلة جدا أو خفيفة يعني + +295 +00:21:02,770 --> 00:21:05,610 +الـ Immunity ل السيلمونيلة تعيفي الـ VI capsular + +296 +00:21:05,610 --> 00:21:08,950 +antigen وحكينا عنه وقولنا عنه protective ال + +297 +00:21:08,950 --> 00:21:12,450 +diagnosis بيشبه إلى حد كبير الشجلة و احنا اتفقنا + +298 +00:21:12,450 --> 00:21:16,990 +على انه كده هم لما بنتكلم عن typhoid او + +299 +00:21:16,990 --> 00:21:20,850 +angastroenteritis بنتكلم عن مين هنا؟ عن الستول و + +300 +00:21:20,850 --> 00:21:23,650 +بدلا من انتكلم عن الستول يبقى عزل السيلمونيلة بنفس + +301 +00:21:23,650 --> 00:21:27,230 +الطريقة اللي عزلنا فيها الشجلة و انا مش هتكلم عن + +302 +00:21:27,230 --> 00:21:31,090 +التفاصيل الصحية هتخليها لل diagnostic اكتر في + +303 +00:21:31,090 --> 00:21:34,370 +كيفية عزل التنال لكنفي ال enteric fever طلعوا + +304 +00:21:34,370 --> 00:21:37,670 +العينات ال typhoid ال enteric fever اللي هو ال + +305 +00:21:37,670 --> 00:21:41,230 +typhoid و ال parathyroid blood bone marrow stool + +306 +00:21:41,230 --> 00:21:44,010 +urine يعني في عينات مختلفة من ضمن هذا ال stool + +307 +00:21:44,010 --> 00:21:50,350 +food poisoning stool vomits حتى القيق اللي ممكن + +308 +00:21:50,350 --> 00:21:55,540 +يكون عينة و ناخدها و نعزل منهابقايا ال food اللي + +309 +00:21:55,540 --> 00:21:58,820 +هو suspected food كلمة suspected اللي هو الغذاء + +310 +00:21:58,820 --> 00:22:03,920 +المشتبه بيه و الغذاء المشتبه بيه قد يكون يعني + +311 +00:22:03,920 --> 00:22:07,880 +unconsumed او leftover بواقي + +312 +00:22:10,260 --> 00:22:13,280 +طبعا انا في عملتي اللي انا بدرس food microbiology + +313 +00:22:13,280 --> 00:22:16,240 +في مدارس المدستير فبنعلمهم كيف يعملوا + +314 +00:22:16,240 --> 00:22:19,180 +investigation investigation اللي هي عملية تحقيق + +315 +00:22:19,180 --> 00:22:22,500 +وعملية التحقيق في تساوم الغذاء هي عملية تحقيق في + +316 +00:22:22,500 --> 00:22:25,780 +جرائم يعني كأنهم بتحقق في جريمة فبعض الأحيان + +317 +00:22:25,780 --> 00:22:32,950 +الدالج أنه نروح على أكياس القمامةوانطلع منها .. + +318 +00:22:32,950 --> 00:22:36,490 +انطلع منها ال .. ما .. ما .. ما تم استخدامه .. ما + +319 +00:22:36,490 --> 00:22:41,770 +تم استخدامه من بقايا الغذاء تبع ال .. ال .. الناس + +320 +00:22:41,770 --> 00:22:47,730 +اللي قصيبوا به التسمة فكلمة left over food left + +321 +00:22:47,730 --> 00:22:53,250 +over food او suspected food هي عملية مهمة جدا في + +322 +00:22:53,250 --> 00:23:00,590 +اللي هو العينة ال septicemia فيها نهار جدكان عينة + +323 +00:23:00,590 --> 00:23:02,810 +.. عينة واحدة اللي البلاد ضد البكتيريا واللي + +324 +00:23:02,810 --> 00:23:05,810 +موجودة دلوقتي فقط في الدم، مع أنها مش .. مش زي في + +325 +00:23:05,810 --> 00:23:08,270 +الـ Type-IVA اللي موجودة في الانتستين وموجودة في + +326 +00:23:08,270 --> 00:23:10,390 +ال reticulum and arterial system خلتنا نتكلم عن + +327 +00:23:10,390 --> 00:23:15,270 +مين؟ عن بواب مرصح وعن البلاد و .. وكونها بتعمل + +328 +00:23:15,270 --> 00:23:19,070 +circulation يمكن ممكن تصير كمان إيه؟ urinary tract + +329 +00:23:19,070 --> 00:23:24,500 +infectionعملية التعرف بتتم من خلال المزرعة و ال + +330 +00:23:24,500 --> 00:23:28,460 +biochemical identification الفحص الأخير اللي هو C + +331 +00:23:28,460 --> 00:23:33,600 +اسمه Vidal test Vidal مع أنها مكتوبة W لكن طول + +332 +00:23:33,600 --> 00:23:38,060 +الفظ V زي Volkswagen اللي هي السيارة المشهورة + +333 +00:23:38,060 --> 00:23:44,360 +الألمانية ال W عنده طول الفظ بحرف اللي هو V اسمها + +334 +00:23:44,360 --> 00:23:53,100 +يعني Vidal testالـ Vidal Test هو عبارة عن + +335 +00:23:53,100 --> 00:24:02,660 +سيرولوجيكال تست لتكتشف موجودة عضلات تايفي و بارا + +336 +00:24:02,660 --> 00:24:10,140 +تايفي تايفي و بارا تايفي طيب إيش الانتجارات اللي + +337 +00:24:10,140 --> 00:24:11,580 +بندور عليها في ال .. أو ال antibodies اللي بندور + +338 +00:24:11,580 --> 00:24:15,300 +عليها في ال .. في ال .. في ال Vidal؟ بندور على ال + +339 +00:24:15,300 --> 00:24:21,970 +O و ال Hطيب ال O و ال H للسلمونيلة تايفي هي اتنين + +340 +00:24:21,970 --> 00:24:24,630 +يبقى الفيديو اللي بكشف عن اتنين للسلمونيلة تايفي + +341 +00:24:24,630 --> 00:24:30,250 +اللي هم ال O و H بيسموهم O T و H T او T H و T O + +342 +00:24:30,250 --> 00:24:38,310 +تايفي O و تايفي H تمام و بنكشف عن السلمونيلة بره + +343 +00:24:38,310 --> 00:24:44,670 +تايفي A و بره تايفي B و بره تايفي Cنفس الكلام اللي + +344 +00:24:44,670 --> 00:24:50,210 +حكيته على الطيفي ان في عندي O و في عندي H بتكلم عن + +345 +00:24:50,210 --> 00:24:56,130 +ال A و ال B و ال C في الفرعة طيفي فبقول الفيديو + +346 +00:24:56,130 --> 00:25:04,430 +اللي بيكشف عن T O T H طيفي O طيفي H A O A H B O B + +347 +00:25:04,430 --> 00:25:09,870 +H C O C H اكمل انتي و بعدين سهرون؟ ستة و اتنين + +348 +00:25:09,870 --> 00:25:15,010 +تمانية تمانية في غزة ال Cبقولك هي بتنتقل على طريق + +349 +00:25:15,010 --> 00:25:19,250 +الخنازير فمش موجودة فبستخدموها فقط تبيكشفوه عن + +350 +00:25:19,250 --> 00:25:23,390 +تلاتة تايفي و عن بارا تايفي A و بارا تايفي B كل + +351 +00:25:23,390 --> 00:25:27,230 +واحد منهم two antigens وبتالي six antigens للكشف + +352 +00:25:27,230 --> 00:25:30,910 +عن six antibodies مورودة في جسم الإنسان طبعا + +353 +00:25:30,910 --> 00:25:33,130 +الفيديو اللي عليه أعلن استفاهام كثيرة من بعض + +354 +00:25:33,130 --> 00:25:37,510 +الباحثين أنه useless لكن مجرد هو أحد الفعاليات + +355 +00:25:37,510 --> 00:25:40,970 +المهمة جدا في قطة غزة للتعرف على ال infection + +356 +00:25:40,970 --> 00:25:48,150 +بالسنوات تايفي والبارا تايفينجي لتحص مابقوا + +357 +00:25:48,150 --> 00:25:51,290 +ماشوفوهش طول سنة تانية، ماشوفوهوش لسه اللي هو + +358 +00:25:51,290 --> 00:25:56,010 +triple sugar iron اجل، و هذا بده شرح و بده تركيز + +359 +00:25:56,010 --> 00:26:00,070 +كويس عشان نستوى، طبعا هو هتشرح في المعمل و + +360 +00:26:00,070 --> 00:26:05,310 +هتشتغلوا بالمعمل، شغل بيه، لكن لبقى انه نستوي منه + +361 +00:26:08,970 --> 00:26:12,530 +في المنطقة هذه القنبوبة هذه القنبوبة اللي هي ال + +362 +00:26:12,530 --> 00:26:16,170 +control يعني البكتيريا اللي مش مزرعة اللي مش مزرعة + +363 +00:26:16,170 --> 00:26:22,190 +عبارة عن أيش مكوّنة من أيش من slant و butt يارتن + +364 +00:26:22,190 --> 00:26:28,790 +هحط على لوحة slant و butt الآن بتزرع البكتيريا عن + +365 +00:26:28,790 --> 00:26:32,450 +طريق حقنها في ال butt و من ثم زيجزاج على ال slant + +366 +00:26:32,450 --> 00:26:33,390 +كويس؟ + +367 +00:26:37,270 --> 00:26:41,450 +الجزء الصفلي هذا بنسميه بط واللي قلوي بنسميه slant + +368 +00:26:41,450 --> 00:26:46,170 +الديب لما يكون الأنبوبة كلها مافيش فيها slant + +369 +00:26:46,170 --> 00:26:53,050 +مافيش فيها slant اتفقنا على انه بنعمل step و بعدين + +370 +00:26:53,050 --> 00:26:56,030 +زيك زيك على ال surface طب ايش فيها الوصفة اسم + +371 +00:26:56,030 --> 00:26:58,510 +الوصفة triple sugar iron agar في تلات سكريات تلات + +372 +00:26:58,510 --> 00:27:03,860 +سكرياتاحفظها مثلا على أصابع يعني لجلوكوز لكتوز + +373 +00:27:03,860 --> 00:27:07,800 +ساكروز على أصابع لأن هذا أصبع صغير تمام؟ كمية + +374 +00:27:07,800 --> 00:27:14,160 +لجلوكوز هي الأقل وال لكتوز و الساكروز كميتهم واحدة + +375 +00:27:14,160 --> 00:27:19,900 +تمام؟ هذا point واحد في المية لجلوكوز والتاني واحد + +376 +00:27:19,900 --> 00:27:25,900 +في المية واحد واحد اظن يعني أكتر أضعف لجلوكوز طيب + +377 +00:27:25,900 --> 00:27:29,510 +إيش الحكمة من ذلك؟ هنشوفها كمان شويةحطينا في ال + +378 +00:27:29,510 --> 00:27:34,710 +media pH Indicator pH Indicator فاكرين البكتيريا + +379 +00:27:34,710 --> 00:27:38,350 +اللي زرعناها ستاب و عملنا زجزاج و حطيناها في ال + +380 +00:27:38,350 --> 00:27:44,330 +incubator بعد 24 ساعة اكتشفنا ان البكتيريا بتخمر + +381 +00:27:44,330 --> 00:27:49,270 +الجلوكوز فقط كيف عرفنا ان البكتيريا بتخمر الجلوكوز + +382 +00:27:49,270 --> 00:27:55,710 +عرفنا عن طريق كمية الأسنين اللي انتجتكمية ال acid + +383 +00:27:55,710 --> 00:28:00,450 +تتنسب مع مين اتذكروا ال glucose كان هو الأصل فإذا + +384 +00:28:00,450 --> 00:28:06,410 +تحول فقط الجزء السفلي زي في الموبا لقم تلاتة إلى + +385 +00:28:06,410 --> 00:28:10,030 +اللون الأصفر اعرفوا أن فقط ال glucose هو اللي + +386 +00:28:10,030 --> 00:28:17,780 +اتخمرلكن لو البكتيريا خمرت لجلوكوز و أحد السكرين + +387 +00:28:17,780 --> 00:28:21,180 +او كلا هما .. أحد السكرين هما اللاكتروز او السكروز + +388 +00:28:21,180 --> 00:28:25,260 +او التلان مع بعض يعني التلانة كلهم اتخمروا فحلاجي + +389 +00:28:25,260 --> 00:28:29,560 +ان الأنبوبة كلها من أولها لأخرها يصار صفر تحولت + +390 +00:28:29,560 --> 00:28:32,240 +إلى اللون الأصفر نتيجة طبعا كمية ال acid اللي هو + +391 +00:28:32,240 --> 00:28:35,580 +الكمية يبقى لما اشوف أنبوبة كلها صفر عايش مع + +392 +00:28:35,580 --> 00:28:40,480 +الحكيملأ اسمع الكلام اللي بدي أحكيه كويس عشان أنا + +393 +00:28:40,480 --> 00:28:46,660 +أكون دقيق معاكم أول حاجة بأكدها أنه خمرت الجلوكوز + +394 +00:28:46,660 --> 00:28:49,700 +هذا مؤكد لأنه مش مستحيل تخمر اللبتوس من دون ما + +395 +00:28:49,700 --> 00:28:55,240 +تخمر الجلوكوز، إذا خمرت مين قلنا؟ الجلوكوزالقادة + +396 +00:28:55,240 --> 00:29:00,460 +التانية يا إما .. يا إما خمرت اللاكتوز لحاله أو + +397 +00:29:00,460 --> 00:29:04,280 +الساكروس لحاله أو التنرمابا، مافقدرش أي ذنب، بس + +398 +00:29:04,280 --> 00:29:10,060 +المؤكد إن خمرت اللاكتوز واحد السكرين أو كلهم، طيب، + +399 +00:29:10,060 --> 00:29:13,720 +اللوحة مرة تانية، شايفين الصورة من تحت من هنا؟ فيه + +400 +00:29:13,720 --> 00:29:14,400 +فراغ، في الأمام + +401 +00:29:17,510 --> 00:29:22,450 +مؤشرك أنت لدي البكتيريا غاز فالغاز رفع ال media + +402 +00:29:22,450 --> 00:29:25,830 +إلى أعلى فبقدر أقول إن البكتيريا هنا خمرت من ال + +403 +00:29:25,830 --> 00:29:30,130 +glucose و أحد ال sucrine أو كلا هما و أنت جد غاز و + +404 +00:29:30,130 --> 00:29:35,550 +غاز بالمظلوم حرف ال G عفوا طيب في الأنبوبة رقم + +405 +00:29:35,550 --> 00:29:39,730 +أربعة لأ في الأنبوبة رقم اتنين عفوا بلاحظ إن + +406 +00:29:39,730 --> 00:29:43,390 +البكتيريا نامي على ال slant هنا تمام لكن مافيش + +407 +00:29:43,390 --> 00:29:47,800 +تغيير في اللون لا فوق ولا تحتيجي البكتيريا non + +408 +00:29:47,800 --> 00:29:53,500 +-glucose fermenter تذكروا ال enterobacteriasi كلها + +409 +00:29:53,500 --> 00:29:57,500 +glucose fermenter والبعض الآخر منها lactose حتى + +410 +00:29:57,500 --> 00:30:01,520 +كمان فال .. فال .. فال enterobacteriasi على الأقل + +411 +00:30:01,520 --> 00:30:04,120 +لازم تعطي .. على الأقل لازم تعطيني yellow but + +412 +00:30:04,120 --> 00:30:07,720 +الجزء الصفر لازم تكون ايه؟ أصفر يعني السيرمونولا + +413 +00:30:07,720 --> 00:30:11,460 +تبعتنا اللي هي non-lactose fermenter لازم تكون + +414 +00:30:11,460 --> 00:30:18,270 +حمره من فوق و من تحت ايه؟ صفرK أحمر A أصفر + +415 +00:30:18,270 --> 00:30:24,670 +فبنسميها K على A K alkaline يعني و A أسفر ف K على + +416 +00:30:24,670 --> 00:30:29,630 +A وصلت الصورة K على A السلمونيلا الان في جمال نقطة + +417 +00:30:29,630 --> 00:30:35,650 +اسم الوضع triple sugar iron agar وضعنا iron في ال + +418 +00:30:35,650 --> 00:30:39,250 +media ليش وضعنا iron؟ في بعض البكتيريا بنات تنتج + +419 +00:30:39,250 --> 00:30:46,410 +H2S as a resultبنتج H2S، الآن الـ H2S غاز عادي من + +420 +00:30:46,410 --> 00:30:50,810 +اللون، مالوش لون، عشان أن در أكشفه حطتله حديد + +421 +00:30:50,810 --> 00:30:53,550 +بيرتلط مع الحديد تكون لون أسود + +422 +00:31:03,820 --> 00:31:09,740 +كيف قلت A وهي لونها أسود؟ كيف قلت A وهي لونها + +423 +00:31:09,740 --> 00:31:16,960 +أسود؟ كيف قلت A وهي لونها أسود؟ كيف قلت A وهي + +424 +00:31:16,960 --> 00:31:23,260 +لونها أسود؟ كيف قلت A وهي لونها أسود؟ كيف قلت A + +425 +00:31:23,260 --> 00:31:25,380 +وهي لونها أسود؟ كيف قلت A وهي لونها أسود؟ كيف قلت + +426 +00:31:25,380 --> 00:31:26,660 +قلت A وهي لونها أسود؟ كيف قلت A وهي لونها أسود؟ + +427 +00:31:26,660 --> 00:31:28,340 +كيف قلت A وهي لونها أسود؟ كيف قلت A وهي لونها + +428 +00:31:28,340 --> 00:31:30,520 +أسود؟ كيف قلت A وهي لونها أسود؟ كيف قلت A وهي + +429 +00:31:30,520 --> 00:31:30,580 +لونها أسود + +430 +00:31:33,880 --> 00:31:37,520 +الـTriple Sugar Iron Agar من أرواح الفحصات البيو + +431 +00:31:37,520 --> 00:31:39,840 +كيميائية اللي تعطينا initial differentiation يعني + +432 +00:31:39,840 --> 00:31:42,960 +انا مثلا لو شفت اللموبة هذه البكتيريا كانت عندي + +433 +00:31:42,960 --> 00:31:46,040 +gram negative بس ال lie وشفت اللموبة هذه بقول عن + +434 +00:31:46,040 --> 00:31:49,880 +البكتيريا non-fermented يا أسينيتوباكتات فاكرين + +435 +00:31:49,880 --> 00:31:53,360 +الـnon-fermented؟ يا أسينيتوباكتات يا Sodomonas + +436 +00:31:53,360 --> 00:31:57,220 +بميز منهم بفحص صغير باخد قُلص أكسيدايزر بحطه على + +437 +00:31:57,220 --> 00:31:59,620 +الـTriple Sugar Iron Agar والله إذا purple فهي + +438 +00:31:59,620 --> 00:32:03,000 +Sodomonas إذا non-purple فهي أسينيتوباكتات + +439 +00:32:05,620 --> 00:32:14,000 +سيتعرف على البكتيريا خارقنا بفحصات بطيطة طيب احد + +440 +00:32:14,000 --> 00:32:17,500 +الميكروبات اللطيفة اسمها Eclipsella والصورة اللي + +441 +00:32:17,500 --> 00:32:22,820 +قدامكوا بتوضح انها Eclipsella ايش الصورة بتقول؟ + +442 +00:32:22,820 --> 00:32:26,060 +بتقول ان البكتيريا عادي نامية على الماكنكي نامية و + +443 +00:32:26,060 --> 00:32:28,020 +lactose fermenter كيف انا عرفت ان انا lactose + +444 +00:32:28,020 --> 00:32:33,310 +fermenter؟ لان هو المستعمرات pink زهنيطيب كيف عرفت + +445 +00:32:33,310 --> 00:32:38,230 +إنها encapsulated؟ المستعمرات ميكويد و large لأن + +446 +00:32:38,230 --> 00:32:43,530 +البكتيريا حجم إلا حجم و ال capsule ضعف أو ضعفاية + +447 +00:32:43,530 --> 00:32:47,750 +حجم البكتيريا فالمستعمر اللي فيها مليون هتعطي حجم + +448 +00:32:47,750 --> 00:32:51,990 +محدد لو قرنناها بال equalize و كانت تلت مليون أو + +449 +00:32:51,990 --> 00:32:56,970 +خمس مليونبتكون الحاجة دايما ايه؟ أكبر و لإنه + +450 +00:32:56,970 --> 00:33:00,410 +الكابسول مكوّلة من polysaccharide و سمينا ميوكو + +451 +00:33:00,410 --> 00:33:03,670 +بوليساكراد حتى فهي بتكون ميوكويت كورنس + +452 +00:33:08,130 --> 00:33:11,850 +كليبسيلا نيومونيا براديا الأشهر أطلاق كليبسيلا + +453 +00:33:11,850 --> 00:33:14,870 +نيومونيا في طبع سبيسيس أخرى زي ال + +454 +00:33:14,870 --> 00:33:19,290 +rhinosceleromatis وكليبسيلا أوزوني أوزوني يعني + +455 +00:33:19,290 --> 00:33:22,710 +واضح أنه العلاقة بالأوزون و rhino .. rhino يعني + +456 +00:33:22,710 --> 00:33:26,130 +الأنف و sclera .. sclera اللي هي الشيء اللي ممكن + +457 +00:33:26,130 --> 00:33:30,520 +تعمل infection بالعينMay cause primary pneumonia + +458 +00:33:30,520 --> 00:33:33,620 +كلمة primary pneumonia عكس secondary فاكرين + +459 +00:33:33,620 --> 00:33:36,700 +secondary؟ مين بجي يسبب secondary pneumonia؟ + +460 +00:33:36,700 --> 00:33:39,760 +streptococcus pneumonia سببت secondary pneumonia + +461 +00:33:39,760 --> 00:33:43,540 +due to viral infection صح ولا لأ فانت تعمل ال + +462 +00:33:43,540 --> 00:33:45,980 +primary in pneumonia ممكن تعمل ال urinary tract + +463 +00:33:45,980 --> 00:33:51,120 +infection one infection bacteremia and meningitis + +464 +00:33:51,120 --> 00:33:55,060 +في كان دراسة عملناها قبل فترة عن الكليبسيلا + +465 +00:33:55,060 --> 00:34:00,820 +نيومونيا في ال ice creamوجدنا إنه نسبة كبيرة من ال + +466 +00:34:00,820 --> 00:34:07,160 +ice cream اللي بيباع في المحلات في قطاع غزة بيحتوي + +467 +00:34:07,160 --> 00:34:11,360 +على clips اللي ال pneumonia طبعا كان الغرض من + +468 +00:34:11,360 --> 00:34:14,280 +الدراسة أن نشوف مدى مقاومتها لاجئنا أن مقاومتها + +469 +00:34:14,280 --> 00:34:18,500 +كويسة مش نمش نمش مش عاطلة مش زي المستشفيات لكن في + +470 +00:34:18,500 --> 00:34:21,560 +الآخر بيوجدها في ال ice cream و أشهر خطيرة هذا + +471 +00:34:21,560 --> 00:34:25,440 +بيدل على أن ال processing للمنتجات + +472 +00:34:27,170 --> 00:34:29,930 +هتاكلوا عيسى كريم بعدها كده؟ اه انا بتاكل اليوم + +473 +00:34:29,930 --> 00:34:33,190 +طيب + +474 +00:34:33,190 --> 00:34:37,070 +بيعمل + +475 +00:34:37,070 --> 00:34:39,110 +ال .. ال .. ال .. ال Klebsiella اللي قلنا عنها + +476 +00:34:39,110 --> 00:34:42,670 +انقطعها اه Klebsiella rhinoscelerometer + +477 +00:35:02,680 --> 00:35:07,480 +الأنف بيفتح دل الأنف اذا عندنا كلبسلة و طبعا في + +478 +00:35:07,480 --> 00:35:08,280 +كلبسلة + +479 +00:35:13,630 --> 00:35:19,510 +اللي الآن بتعتبر super bug ماهي super bug؟ مصطلح + +480 +00:35:19,510 --> 00:35:23,610 +بيطلقوه في العامة .. العامين على ميكروب اللي له + +481 +00:35:23,610 --> 00:35:27,990 +قدرات خارقة على مقاومة كل الموضوعات الحيوية أحد + +482 +00:35:27,990 --> 00:35:32,430 +أشهر ال super bugs الآن في العالم كليبسيلة و + +483 +00:35:32,430 --> 00:35:37,490 +بيسموها carbamium resistant klebsiella + +484 +00:35:40,780 --> 00:35:46,040 +من بارح اعتقد انا نزلت post على ال facebook من .. + +485 +00:35:46,040 --> 00:35:50,400 +من الهند، كاتبان New York Times عن نسبة الوفيات + +486 +00:35:50,400 --> 00:35:54,980 +اللي زادت بين ال infants في الهند، في الهند، + +487 +00:35:54,980 --> 00:35:59,520 +كنتيجة على إصابتهم بست سيمية ب super bug، ب super + +488 +00:35:59,520 --> 00:36:03,900 +bug، من أشهر ال ..وحتى انها تغزها موجود ان + +489 +00:36:03,900 --> 00:36:08,100 +كيبسيلانيومونيا that are multiple drug resistant + +490 +00:36:08,100 --> 00:36:11,980 +ومنها كربابنيم resistant او كربابنيميز producer + +491 +00:36:11,980 --> 00:36:16,100 +ففي خطورة كبيرة من هذه البكتيرا نيجي للبكتيرا + +492 +00:36:16,100 --> 00:36:20,160 +الجميلة اللى هى ال protease اللى تعرف الدوائر + +493 +00:36:20,160 --> 00:36:25,460 +تبعتها اللى هى عملة ولمش نبينها دوائر نبينهاطبعا + +494 +00:36:25,460 --> 00:36:27,960 +كيف حصلنا على طبقها ده احنا بنجيب ال eclipse اللي + +495 +00:36:27,960 --> 00:36:30,700 +هو بنزرعها في منتصف الطبق في منتصف الطبق لما نعيش + +496 +00:36:30,700 --> 00:36:34,700 +زيك زاك بس بنحطها كنقطة في منتصف الطبق و بنسيبها + +497 +00:36:34,700 --> 00:36:40,780 +24 ساعة البكتيريا بتنمو تنمو و بتتكاثر في المنطقة + +498 +00:36:40,780 --> 00:36:45,700 +فبيخلص الغدا .. بيخلص الغدا فبتروحوا هي عاملة + +499 +00:36:45,700 --> 00:36:53,240 +quorum sensing ننتقلننتقل الى ان نبدأ ننتج او + +500 +00:36:53,240 --> 00:36:57,040 +نستخلص الماء او نعمل secretion ال water فبصير فين + +501 +00:36:57,040 --> 00:37:02,160 +زي قطرة ماء على المستعمرة بتسبح فيها الميكروبتان + +502 +00:37:02,160 --> 00:37:05,000 +وميناركو الموطنة التي بقت البكتيريا هي swimming + +503 +00:37:05,000 --> 00:37:08,120 +وبالتالي الأجار solid مابتقدرش تمشي عليه ماشي + +504 +00:37:08,120 --> 00:37:13,920 +فمضطرة تنتج ال water فتسبح فيه احد ميزات الأجار + +505 +00:37:13,920 --> 00:37:19,480 +انه بياخد الميهفمش هتنتقل لمسافة فويلة لكن غطت + +506 +00:37:19,480 --> 00:37:25,740 +مساحة لم تكن قد وصلتها من قبل فبتبتد كعتر بيخلص + +507 +00:37:25,740 --> 00:37:29,600 +اللي بيبقى نفس الجثة quorum sensing انتقال فبتشوف + +508 +00:37:29,600 --> 00:37:35,660 +الحواف تبعت الدوائر هي حواف جفاف الماء تمام؟ في + +509 +00:37:35,660 --> 00:37:40,180 +المنطقة وانتقال إلى مرحلة جديدة لكن هيك الببتيريا + +510 +00:37:40,180 --> 00:37:41,420 +غطت كل + +511 +00:37:47,270 --> 00:37:50,250 +يعني لو انا عملتلكوا في ال .. في ال .. في المعمل + +512 +00:37:50,250 --> 00:37:53,570 +unknown، إيش an unknown؟ مش معروف يعني بيبتلكوا + +513 +00:37:53,570 --> 00:37:55,870 +بكتيريا و بيبتلكوا هذه unknown، بيكونوا اتعرفوا + +514 +00:37:55,870 --> 00:37:57,790 +عليها و هذا الكلام بيتم في ال diagnostic في آخر + +515 +00:37:57,790 --> 00:38:01,370 +الفصل الآن + +516 +00:38:01,370 --> 00:38:04,630 +حاططوا لكوا mixture لإنه مش .. مش منطقي لإن انتوا + +517 +00:38:04,630 --> 00:38:06,730 +اكبرتوا تكونوا في ال diagnostic و تشتغلوا عليا .. + +518 +00:38:06,730 --> 00:38:10,220 +عليا بكتيريا واحدة، فحاططوا لكوا mixtureاللي إيجا + +519 +00:38:10,220 --> 00:38:14,080 +من ال mixture تبعهم فيه Protease سيكون هناك صعوبة + +520 +00:38:14,080 --> 00:38:18,620 +كبيرة جدا لهم إنهم يفصلوا ال microbe التاني أو + +521 +00:38:18,620 --> 00:38:21,940 +التالت عن مين عن ال Protease لأن ال Protease هتغطي + +522 +00:38:21,940 --> 00:38:26,120 +على كل المستعمرات اللي موجودة فهنضطر نلجأ إلى ما + +523 +00:38:26,120 --> 00:38:31,700 +يسمى بال selective media selective media أو + +524 +00:38:31,700 --> 00:38:34,780 +selective measures يعني نحط Antibiotics معروفة + +525 +00:38:34,780 --> 00:38:37,600 +بتقتل ال Protease عشان نعزل مين الميكروبيت التاني + +526 +00:38:42,230 --> 00:38:46,490 +في كمان .. في كمان فكرة كويسة عشان أقول إن الأجار + +527 +00:38:46,490 --> 00:38:50,410 +بحب الميه بنزيد نسبة الأجار في ال .. في الميديا + +528 +00:38:50,410 --> 00:38:53,210 +بدل من نخليها .. هي واحد و نص معروف عنها بنخليها + +529 +00:38:53,210 --> 00:38:57,330 +تلت و نص فلان تستطيع البكتيريا .. حد كل مكان بتجنت + +530 +00:38:57,330 --> 00:39:01,710 +في الميه الأجار يعني بياخدها و بيخلصنا منها ال + +531 +00:39:01,710 --> 00:39:05,370 +Protis أبنات من الميكروبات برضه الشريرة يعني من + +532 +00:39:05,370 --> 00:39:09,990 +الميكروبات المشهورة إنها بتعمل Persistent Wound + +533 +00:39:09,990 --> 00:39:13,920 +Infection and Burn Infectionكلمة swarming اللي هي + +534 +00:39:13,920 --> 00:39:17,680 +الظاهرة اللي شفتوها هذه طبعا هذه الظاهرة غالبا ما + +535 +00:39:17,680 --> 00:39:21,100 +بنشوفهاش على المقوم كي بنشوفها وين؟ على ال non + +536 +00:39:21,100 --> 00:39:24,120 +-selective media زي النوتريند أجاري و زي ال vlog + +537 +00:39:24,120 --> 00:39:29,920 +أجر بعض ال species Brutius vulgaris, Brutius + +538 +00:39:29,920 --> 00:39:35,660 +mirabilis و Brutius myoxifasciens myoxifasciens + +539 +00:39:35,660 --> 00:39:40,360 +يبقى فينا mirabilis, vulgaris and myoxifasciens + +540 +00:39:40,990 --> 00:39:45,110 +طبعا ال antigenic structure في عندها O-antigen و H + +541 +00:39:45,110 --> 00:39:47,610 +-antigen لإنه كلها motile و أكبر دي على ال + +542 +00:39:47,610 --> 00:39:50,410 +motility بتاعتها اللي هي ال swarming اللي بيبقى + +543 +00:39:50,410 --> 00:39:55,450 +عنها جابر شوية النقطة التالتة دي بدها شرح ممكن خمس + +544 +00:39:55,450 --> 00:40:02,710 +دقائق نصفر على بعض و نتحملها سمعتوا + +545 +00:40:02,710 --> 00:40:03,790 +براكتسيا؟ + +546 +00:40:12,820 --> 00:40:15,720 +Intracellular ماذا يعني interest obligate interest + +547 +00:40:15,720 --> 00:40:19,460 +obligate يعني + +548 +00:40:19,460 --> 00:40:24,140 +لن تستطيعوا أنكوا تناموها لأ + +549 +00:40:24,140 --> 00:40:28,040 +لن تستطيعوا أنكوا تناموها وين في الأطباق في + +550 +00:40:28,040 --> 00:40:31,560 +الأطباق بدا cell culture بدا cell culture يبقى في + +551 +00:40:31,560 --> 00:40:35,060 +صعوبة في تنميتها في صعوبة في تنميتها هي بتعمل مرض + +552 +00:40:35,060 --> 00:40:43,490 +مشهور جدا اسمه typhus feverعشان نشخص مش typhoid + +553 +00:40:43,490 --> 00:40:49,710 +عشان نشخص ال typhus ممكن نكشف عنه عن ال antibodies + +554 +00:40:49,710 --> 00:40:53,250 +في جسم الإنسان لإنه عزل البكتيريا صعب جدا يبقى + +555 +00:40:53,250 --> 00:40:57,210 +نجاب شوية فممكن نكشف عن الأجسام المضادة طب عشان + +556 +00:40:57,210 --> 00:40:59,850 +نكشف عن الأجسام المضادة بيانتجين الانتجين هو نفس + +557 +00:40:59,850 --> 00:41:04,810 +البكتيريا عشان نميها برا برضه expensive فاكرين + +558 +00:41:04,810 --> 00:41:07,450 +كلمة cross reaction؟ مش cross كنت بتبقى عشان cross + +559 +00:41:07,450 --> 00:41:11,560 +reaction اللي هو ال antibody الواحدقد يتفاعل مع + +560 +00:41:11,560 --> 00:41:20,120 +two antigens حكيناها الظاهرة بشكل كبير الان + +561 +00:41:20,120 --> 00:41:25,140 +البروتياس لقوا انها بتعمل cross reaction مع مين؟ + +562 +00:41:25,140 --> 00:41:32,200 +مع الركيزيا فاستخدمنا البروتياس كانتجينات للكشف عن + +563 +00:41:32,200 --> 00:41:35,780 +ال antibodies بتاعة مين؟ بتاعة الركيزيا الان + +564 +00:41:35,780 --> 00:41:38,840 +الركيزيا بسبب الtyphus الtyphus في بره هم الtyphus + +565 +00:41:38,840 --> 00:41:42,470 +صح؟الليبروتياس اللي علاقة اللي ماضيها ده مانعاش + +566 +00:41:42,470 --> 00:41:45,510 +علاقة لها من قريب ولا من بعيد العلاقة اللي احنا + +567 +00:41:45,510 --> 00:41:48,630 +بنحكيها الآن ان احنا بنستخدم بعض السلالات من + +568 +00:41:48,630 --> 00:41:51,750 +الليبروتياس ده الكشف عن ال antibodies تبع + +569 +00:41:51,750 --> 00:41:56,430 +الركيدسيا ويسمى هذا الفحص wheel Felix reaction + +570 +00:41:56,430 --> 00:42:00,450 +wheel Felix reaction مع علاقة الليبروتياس بلا + +571 +00:42:00,450 --> 00:42:05,370 +reaction فقط هيدستخدم كانتجينات للكشف عنها ليش + +572 +00:42:05,370 --> 00:42:08,390 +استخدمناها لإن وجينا فيها cross reaction مع + +573 +00:42:08,390 --> 00:42:13,030 +الركيدسياطبعا ال strains المشهورة عنها اللي هم ال + +574 +00:42:13,030 --> 00:42:17,870 +Ox-19 واسم الفحص أصلا common-share سموه Ox-19 + +575 +00:42:17,870 --> 00:42:22,350 +هدرسوه في serology ان شاء الله في المعامل Ox-19 و + +576 +00:42:22,350 --> 00:42:26,850 +Ox-K و Ox-2 إذن تلاتة من الأنتجينات اللي بنستخدم + +577 +00:42:26,850 --> 00:42:31,370 +فيهم ال Protis أرجو أنه الكل يعرف أن ال Protis هنا + +578 +00:42:31,370 --> 00:42:34,670 +ليس لها علاقة بالTyphus وإنما استخدمت كوسيلة + +579 +00:42:34,670 --> 00:42:38,890 +للكيشف عنه اللي هو ال antibodyالـ Clinical + +580 +00:42:38,890 --> 00:42:41,930 +Manifestation of Pluteus زيها زي أي انتروبكتريات + +581 +00:42:41,930 --> 00:42:44,570 +بتسبب تقريبا نفس الأمراض اللي بتسبب ال E.coli و E + +582 +00:42:44,570 --> 00:42:48,310 +.eclipsilla لكن most commonly فيه ال urinary tract + +583 +00:42:48,310 --> 00:42:51,390 +infection و تمال wound infection و برضه هي + +584 +00:42:51,390 --> 00:42:56,990 +hospital مع ثوجين و قد تمتلك resistance عالي و انا + +585 +00:42:56,990 --> 00:43:00,050 +بذكر قصة كان حاكيتكوا يا جمادة احد المرضى اللي عمل + +586 +00:43:00,050 --> 00:43:05,270 +skin grafts skin graft يعني زراعة جلب كان متعرض + +587 +00:43:05,270 --> 00:43:10,760 +لحدث و يزرع جلبالان بعض .. بتعرفين الزراعة عبارة + +588 +00:43:10,760 --> 00:43:17,020 +عن جراحة لأ لأ من نفس الشخص هو يعني autograph من + +589 +00:43:17,020 --> 00:43:21,960 +نفس الشخص مافيش فيها rejection لكن جلد و مجروح و + +590 +00:43:21,960 --> 00:43:25,080 +بالتالي فيه إمكانية التعرض ل infection فأصيب بي + +591 +00:43:25,080 --> 00:43:28,720 +infection و طلعت protest و أتسب ال infection في + +592 +00:43:28,720 --> 00:43:35,040 +المستشفيات الداخل في الصين الداخلهذا بدو يتخلص من + +593 +00:43:35,040 --> 00:43:38,800 +القصة هاد يروح المستشفيه يعمل مزرعة لاجوا عنده ال + +594 +00:43:38,800 --> 00:43:42,200 +protease عاملونا sensitivity testing resistant لكل + +595 +00:43:42,200 --> 00:43:46,180 +ال antibiotics اجي عنا ع الجامعة نصيبه يومها كان + +596 +00:43:46,180 --> 00:43:50,100 +عندنا جايينا طلبية من كل ال antibiotics اللي ممكن + +597 +00:43:50,100 --> 00:43:53,420 +يحلم فيها حدا في قطاعنا من الأقراس تلات ال + +598 +00:43:53,420 --> 00:43:56,640 +antibiotics اللي بناها في حصن تحصناه لكل ال + +599 +00:43:56,640 --> 00:43:58,960 +antibiotics اللي مطور في رعينا طلع عنده واحد + +600 +00:43:58,960 --> 00:44:01,080 +sensitive واحد intermediate والباقي كله + +601 +00:44:06,590 --> 00:44:09,530 +عارف نجيبهم من الغارية قصد من الكلام أن ال + +602 +00:44:09,530 --> 00:44:13,110 +protease أيضا من السللاء اللي عندها مفضلة تكوين + +603 +00:44:13,110 --> 00:44:18,530 +resistance عالية هناك بنكون هنا الجزء اللي هو ال + +604 +00:44:18,530 --> 00:44:22,250 +.. ال .. ال .. الأول من ال gram-mated bacilli + +605 +00:44:22,250 --> 00:44:25,130 +المرة اللي جاي هتكلم ان شاء الله عن ال pseudomonas + +606 +00:44:25,130 --> 00:44:27,130 +karygenosa و عن اللي هو ال virus + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vu8Y--lCMY4_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vu8Y--lCMY4_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..657d5e8c83ae4d199e8ea0055d440f542e89f368 --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/vu8Y--lCMY4_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 4575, "start": 20.57, "end": 45.75, "text": "بسم الله الرحمن الرحيم نكمل ان شاء الله من حيث ما انتهينا بمراجعة سريعة على ال physical agents وكان اول physical agent هو ال heat واتفق على ان 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ال", "probability": 0.96484375}, {"start": 333.52, "end": 333.96, "word": " pyrimidines", "probability": 0.9462890625}, {"start": 333.96, "end": 334.16, "word": " هم", "probability": 0.9443359375}, {"start": 334.16, "end": 334.3, "word": " اللي", "probability": 0.988525390625}, {"start": 334.3, "end": 334.56, "word": " بشكل", "probability": 0.9864908854166666}, {"start": 334.56, "end": 335.06, "word": " القوارعة", "probability": 0.569091796875}, {"start": 335.06, "end": 335.32, "word": " تبعت", "probability": 0.7760416666666666}, {"start": 335.32, "end": 336.02, "word": " مين؟", "probability": 0.6673177083333334}, {"start": 336.02, "end": 336.06, "word": " ال", "probability": 0.79150390625}, {"start": 336.06, "end": 336.38, "word": " DNA", "probability": 0.1485595703125}, {"start": 336.38, "end": 338.18, "word": " طب", "probability": 0.85302734375}, {"start": 338.18, "end": 338.26, "word": " ال", "probability": 0.8798828125}, {"start": 338.26, "end": 338.42, "word": " UV", 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00:00:31,110 +اول physical agent هو ال heat واتفق على ان ال heat + +4 +00:00:31,110 --> 00:00:34,530 +ممكن تقسم إلى قسمين القسم الاول كان بيتكلم معانا + +5 +00:00:34,530 --> 00:00:37,470 +wet heat واخدنا مثل على ال wet heat اللي هي ال + +6 +00:00:37,470 --> 00:00:40,270 +boiling و ال boiling زي ال sodium بيكربونات او + +7 +00:00:40,270 --> 00:00:44,890 +بيكربونات ال sodium اللي هي العامية ال carbon اللي + +8 +00:00:44,890 --> 00:00:49,140 +معناها carbon احناوالصنف التاني كان هو ال + +9 +00:00:49,140 --> 00:00:51,340 +pasteurization وشوفنا أشكال مختلفة من ال + +10 +00:00:51,340 --> 00:00:56,840 +pasteurization وشوفنا إنه زيادة عشر درجات مئوية من + +11 +00:00:56,840 --> 00:01:01,680 +62 ل 72 قدرت الزمن من 30 دقيقة إلى 15 ثانية + +12 +00:01:02,670 --> 00:01:07,110 +واتكلمنا ايضا عن نوع اخر منه سميناه ultra-high + +13 +00:01:07,110 --> 00:01:11,830 +treatment pasteurization لكن ولا واحدة من الأول لا + +14 +00:01:11,830 --> 00:01:13,650 +الأولى ولا التانية كانوا بيقدوا الى ال + +15 +00:01:13,650 --> 00:01:16,910 +sterilization لكن التالتة اللى هى كانت steam under + +16 +00:01:16,910 --> 00:01:21,490 +pressure كانت اللى هى ال autoclave أدى الى اللى هو + +17 +00:01:21,490 --> 00:01:25,930 +التعقيم وقلنا at 121Degrees Integrate for 15 + +18 +00:01:25,930 --> 00:01:29,390 +minutes to 20 minutes at 15 psi لو كنا pound per + +19 +00:01:29,390 --> 00:01:35,230 +square inch صنفة تانية كانت من الحلقة الأولى اللي + +20 +00:01:35,230 --> 00:01:37,690 +شكله اللي قدامكوا اللي هو جهاد ال Autoclave بشكل + +21 +00:01:37,690 --> 00:01:42,880 +مبسط عبارة عن heater إذا شايفين تحت هناهذا ال + +22 +00:01:42,880 --> 00:01:48,800 +heater مغمور بالماء و فوق الماء في حاجز هذا حاجز + +23 +00:01:48,800 --> 00:01:53,180 +مثقب يعني يسمح البخار الماء بالخروج و بنضع عليه + +24 +00:01:53,180 --> 00:01:56,020 +الأشياء اللي بنعقمها يعني لو بنعقم إشي معين مثلا + +25 +00:01:56,020 --> 00:02:00,920 +media أو glassware بنحطهم على هذا السطح و بنغلق + +26 +00:02:00,920 --> 00:02:06,100 +بإغلاق محكم يعنيلأن احنا بنتكلم عمين عن بخار ماء + +27 +00:02:06,100 --> 00:02:10,640 +وبالتالي البخار الماء لازم يضلوا في ال container + +28 +00:02:10,640 --> 00:02:13,720 +نفسه وما يتسيرش له تسريب عشان يعطي الحرارة اللي + +29 +00:02:13,720 --> 00:02:17,360 +احنا بدنا يهاليها 121 درجة، مئوية لكي نشكل ضغط + +30 +00:02:17,360 --> 00:02:20,660 +بالأحرار و يترفع لنا الحرارة الشكل اللي أمامكوا + +31 +00:02:20,660 --> 00:02:25,120 +هذا هو الشكل الحقيقي laptop view of an autoclave، + +32 +00:02:25,120 --> 00:02:28,680 +هذا هتشوفوه في المختبر ان شاء الله عندنالحظوا + +33 +00:02:28,680 --> 00:02:36,060 +الغطاء اللى فوق هنا يعني بدنا نبدل قوة كبيرة عشان + +34 +00:02:36,060 --> 00:02:43,080 +نغلق الجهاز اغلاق محكم الشراط اللى بقى بنا هنا هى + +35 +00:02:43,080 --> 00:02:47,220 +أزرار للتشغيل وساعات من خلالها بنعرف الضغط وبنعرف + +36 +00:02:47,220 --> 00:02:53,660 +ال time وبنعرف ال temperature طبعا ال modern + +37 +00:02:53,660 --> 00:02:58,870 +autoclave اتطورت شويةصار فيها إمكانية أنه .. يعني + +38 +00:02:58,870 --> 00:03:01,410 +automated cycles يعني إيش .. إيش .. إيش يعني + +39 +00:03:01,410 --> 00:03:04,910 +automated cycles؟ إحنا بنبرمجها .. بقى .. بنقوله + +40 +00:03:04,910 --> 00:03:07,690 +cycle one أو cycle two حسب البرمج اللي موجودة و + +41 +00:03:07,690 --> 00:03:12,930 +خلاص نترك ال .. ال .. الجهاز لوحده بيجوم هو بيرفع + +42 +00:03:12,930 --> 00:03:16,690 +درجة الحرارة 121 درجة معوية و بيبدأ بعد ذلك يعد + +43 +00:03:16,690 --> 00:03:21,350 +لما ت .. تصل الحرارة 121 بيبدأ يعد الزمن اللي أنا + +44 +00:03:21,350 --> 00:03:24,690 +مخصصه له أو مبرمجه له في ال cycle خمس عشر أو عشرين + +45 +00:03:24,690 --> 00:03:30,300 +دقيقةلو درجة الحرارة نزلت عن .. طبعا بفصل على 121 + +46 +00:03:30,300 --> 00:03:35,780 +الحرارة بتفصل لو نزلت عن 121 بعود بشتغل مرتين لو + +47 +00:03:35,780 --> 00:03:40,420 +ارتفعت عن 121 بفصل وبالتالي بيظل محافظ على درجة + +48 +00:03:40,420 --> 00:03:45,440 +معددة للزمن اللي احنا حدد في كمال modification انه + +49 +00:03:45,440 --> 00:03:48,160 +.. طب احنا بدنا نكون ولا wet wet يعني الأشياء + +50 +00:03:48,160 --> 00:03:53,640 +بتطلع من الرطبة في autoclaves زودت من ما يسمى + +51 +00:04:09,040 --> 00:04:12,260 +الصينف التاني كان من ال .. من ال physical agent + +52 +00:04:12,260 --> 00:04:15,300 +اللي هو radiation و بنقسمه لقسمين برضه، القسم + +53 +00:04:15,300 --> 00:04:18,860 +الأول بتكلم على ionizing radiation والتاني عن non + +54 +00:04:18,860 --> 00:04:23,000 +-ionizing radiationالأولى بيمثلها اللى هو ال .. ال + +55 +00:04:23,000 --> 00:04:27,260 +.. ال .. ال X-ray و التانى بيمثلها ال ultraviolet + +56 +00:04:27,260 --> 00:04:32,380 +light ناخد ال ultraviolet light ال UV يعني من ال + +57 +00:04:32,380 --> 00:04:36,640 +.. ال .. ال .. الإشعاعات اللى أنتوا بتعرفوا أنها + +58 +00:04:36,640 --> 00:04:40,800 +مؤضية للإنسان و بالذات مؤضية للعين ده ال corner of + +59 +00:04:40,800 --> 00:04:45,400 +the eye و مؤضية للجلد و بتسمعوا أنه ثقب ال ozone + +60 +00:04:47,320 --> 00:04:50,540 +سبب بإنه كمية كبيرة من ال UV اللي جاي من الشمس + +61 +00:04:50,540 --> 00:04:53,880 +تدخل الأرض، يعني كان هو يحدث ال UV هذه، فنسبة ال + +62 +00:04:53,880 --> 00:04:57,280 +skin cancer زادت، يعني معناه الحكي إنه أنا ماجعش + +63 +00:04:57,280 --> 00:05:01,340 +أستخدم ال UV بيننا، يعني زي ال neon أو الإضاءة + +64 +00:05:01,340 --> 00:05:04,340 +اللي موجودة هنا، و أحنا بنشتغل في المستشفى أو في + +65 +00:05:04,340 --> 00:05:07,600 +المطبخ، تكون شغالة أحنا بنشتغل معاها، لأ لإنها + +66 +00:05:07,600 --> 00:05:10,480 +مؤدية للعين و بنا مؤدية للجلد و ممكن تعمل بنا + +67 +00:05:10,480 --> 00:05:18,220 +cancerشوفوا ال wavelength المحدد 250 ل 260 نانومتر + +68 +00:05:18,220 --> 00:05:21,860 +هذا ال wavelength الأفضل اللي بيكون فيه قتل على ما + +69 +00:05:21,860 --> 00:05:24,540 +يكون عند هذا ال wavelength والسبب بتكونوا تعرفوا + +70 +00:05:24,540 --> 00:05:30,420 +ليش السبب السبب إنه هذا ال wavelength هو الأفضل لل + +71 +00:05:30,420 --> 00:05:33,080 +absorbance لل purines و ال pyrimidines بتعرفوا ال + +72 +00:05:33,080 --> 00:05:35,060 +purines و ال pyrimidines هم اللي بشكل القوارعة + +73 +00:05:35,060 --> 00:05:40,610 +تبعت مين؟ ال DNA طب ال UV إيش بتاعة مع ال UV؟ال UV + +74 +00:05:40,610 --> 00:05:45,650 +بتشوف اتنين ثايمين جنبها، ثايمين اللي هو ال T، + +75 +00:05:45,650 --> 00:05:49,050 +بتروحوا يعملوا cross link بينهم، ال UV بتعمل cross + +76 +00:05:49,050 --> 00:05:55,170 +link بينهم، فالان هم مش ثايمين، هم ثايمين دايمةالـ + +77 +00:05:55,170 --> 00:05:58,710 +DNA polymerase أو الـ RNA polymerase ما بيقف يقرأ + +78 +00:05:58,710 --> 00:06:03,050 +إلا الأحرف الأربع تبعونه أدنين، جوانين، سايتسين + +79 +00:06:03,050 --> 00:06:06,630 +وثايمين الآن لما بشوف التانين مع بعض مربوطين بعض + +80 +00:06:06,630 --> 00:06:11,390 +انهم ثايمين أو ان أصلهم ثايمين طب يقفز عنهم طب إذا + +81 +00:06:11,390 --> 00:06:14,450 +قفز عنهم مش ستصبح لنا deletion طب إذا صار لنا + +82 +00:06:14,450 --> 00:06:18,450 +deletion مش ستصبح لنا frame shift mutation طيب + +83 +00:06:18,450 --> 00:06:22,490 +تخيلوا الآن كل ما زادت الزمن زمن التعرض ال explore + +84 +00:06:22,490 --> 00:06:27,980 +timeكلما زاد فرصة من الصوفيا الناه ال mutation طب + +85 +00:06:27,980 --> 00:06:30,680 +إذا صار ال mutation في أكتر من مكان وصار ال + +86 +00:06:30,680 --> 00:06:33,980 +mutation في essential genes، يعني جينات أساسية أو + +87 +00:06:33,980 --> 00:06:37,480 +رئيسية لحياة الخلية، يبقى إيش يصير في الخلية الآن؟ + +88 +00:06:37,480 --> 00:06:43,300 +بتموت، هيك عرفنا كيف ال UV بتقتل، إذا كلما زاد زمن + +89 +00:06:43,300 --> 00:06:49,520 +التعرض كلما زاد فرصة موت، اللي هو البكليطبعا معروف + +90 +00:06:49,520 --> 00:06:53,500 +أن قدرة تأثيره على قتل ال vegetative cells أعلى + +91 +00:06:53,500 --> 00:06:59,320 +بكثير من قدرة قتله على ال spores و قد نحتاج يا + +92 +00:06:59,320 --> 00:07:04,000 +بنات عشرة أضعاف الجرعة اللي نحتاجها لمين؟ لل + +93 +00:07:04,000 --> 00:07:06,960 +vegetative cell، يعني ال spores are more resistant + +94 +00:07:06,960 --> 00:07:10,560 +than vegetative cells for UV كده + +95 +00:07:14,830 --> 00:07:19,230 +في كمان ميكانيزم لكن أقل همية للـUV إنه ممكن تضيف + +96 +00:07:19,230 --> 00:07:22,570 +هيدروكسيل جروب لقواعد نيتروجينية وبالتالي بتعمل + +97 +00:07:22,570 --> 00:07:28,300 +برضه destruction لمين؟ للـDNAال .. ال .. ال .. ال + +98 +00:07:28,300 --> 00:07:29,180 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +99 +00:07:29,180 --> 00:07:29,220 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +100 +00:07:29,220 --> 00:07:29,700 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +101 +00:07:29,700 --> 00:07:29,800 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +102 +00:07:29,800 --> 00:07:30,260 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +103 +00:07:30,260 --> 00:07:35,580 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +104 +00:07:35,580 --> 00:07:37,620 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +105 +00:07:37,620 --> 00:07:38,260 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +106 +00:07:38,260 --> 00:07:38,260 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +107 +00:07:38,260 --> 00:07:39,760 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +108 +00:07:39,760 --> 00:07:41,860 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +109 +00:07:41,860 --> 00:07:41,860 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +110 +00:07:41,860 --> 00:07:48,220 +.. ال .. ال .. ال .. ال .. ال .. + +111 +00:07:48,220 --> 00:07:54,180 +ال .. + +112 +00:07:54,220 --> 00:07:58,380 +بيكونوا تعرفوا ان ال repair system هذا فيه منه + +113 +00:07:58,380 --> 00:08:02,820 +error free ايش يعني error free؟ يعني زي ال + +114 +00:08:02,820 --> 00:08:07,720 +mechanic الشاطر بيرجع الخراب كما هو، يعني بصلحه، + +115 +00:08:07,720 --> 00:08:13,940 +ده صحيح، خالي من الخطأ وفي قالية أخرى اسمها error + +116 +00:08:13,940 --> 00:08:21,300 +prone يعني قد يصحي الخطأ بخطأ أخريعني مش دايما + +117 +00:08:21,300 --> 00:08:25,440 +التصحيح ايه بيكون دقيق وإلا ما كان .. وإلا ما كان + +118 +00:08:25,440 --> 00:08:28,000 +مصر عندنا ميوتين بالمرة مدهن .. مدهن فيه repair + +119 +00:08:28,000 --> 00:08:32,440 +mechanism وفي repair mechanism بتتم من خلال الضوء + +120 +00:08:32,440 --> 00:08:36,560 +بيسموها photo repair وفي mechanism لل repair بتتم + +121 +00:08:36,560 --> 00:08:40,400 +في الظلام وبيسميها dark repair يعني في photo + +122 +00:08:40,400 --> 00:08:44,320 +reactivation وفي عندنا اللي هو ال dark repair لكن + +123 +00:08:44,320 --> 00:08:51,500 +بتدريفهم شغل ان ال UV بي ..واقعها وإشكالياتها + +124 +00:08:51,500 --> 00:08:59,380 +المتعلقة بال .. بال .. بال skin damage و بال eye + +125 +00:08:59,380 --> 00:09:04,660 +damage في الحالتين هذا منع استخدام .. منع استخدام + +126 +00:09:04,660 --> 00:09:09,540 +ال UV بكثرة في الطب تعالوا ناخد بعض الأمثلة على + +127 +00:09:09,540 --> 00:09:14,340 +استخدامات ال UV في الطب واحد operating room + +128 +00:09:14,340 --> 00:09:20,740 +disinfection أو sterilizationعن طريق وضع نيونات + +129 +00:09:20,740 --> 00:09:27,720 +لكن هل بنقدر نشتغل و النيونات هي مضارة متى بنشتغل + +130 +00:09:27,720 --> 00:09:33,380 +بعد الانتهاء بمعنى آخر قبل البدء بعمليات جديدة + +131 +00:09:33,380 --> 00:09:37,860 +ممكن نعقم الغرفة عن طريق ال UV light في كمان + +132 +00:09:37,860 --> 00:09:42,060 +استخدام مش مذكور على اللوحة اللي هو في ال water + +133 +00:09:42,060 --> 00:09:47,440 +disinfection تخيلوا معايا الوضع التاليمجرة مائي + +134 +00:09:47,440 --> 00:09:52,480 +سمك الماء فيه يعني عمق الماء بمعنى أخر فيه رقيق + +135 +00:09:52,480 --> 00:10:00,100 +جدا و بيمر عبر مجرة مائي طويل كله مغطى ب UV Lights + +136 +00:10:00,100 --> 00:10:05,660 +فخلينا نقول إن الماء بتعرض لمسافة طويلة كلمة مسافة + +137 +00:10:05,660 --> 00:10:08,480 +ما عند الحكي عنه إن الماء اللي هنا لما يقطع + +138 +00:10:08,480 --> 00:10:14,310 +المسافة بده إيه؟ بده زمن ففيه زمن تعرض معروففممكن + +139 +00:10:14,310 --> 00:10:18,610 +برضه نستخدم ال UV for water disinfection، water + +140 +00:10:18,610 --> 00:10:21,310 +disinfection، في المعانل بتعرفوا اللي أنا في ال + +141 +00:10:21,310 --> 00:10:23,950 +law hood، هذه ال .. ال .. ال .. ال safety cabinet، + +142 +00:10:23,950 --> 00:10:28,470 +بيكون فيها برضه UV، بعد ما بنخلص شغلنا، بننزل ال + +143 +00:10:28,470 --> 00:10:32,010 +.. ال plastic shield، بنشغل ال UV، حتى نعقم ال + +144 +00:10:32,010 --> 00:10:35,720 +area اللي كنا نشتغل فيهاإذا ال UV له استخدامات + +145 +00:10:35,720 --> 00:10:40,360 +جيدة في الطب لكن كمان مرة كان ممكن ممتاز لو احنا + +146 +00:10:40,360 --> 00:10:44,580 +قدرنا نشتغل بال UV موجود بس للأسف لإنه بدورنا + +147 +00:10:44,580 --> 00:10:50,200 +ماقدرنا اليوم في على فكرة تقنية شبيهة طلعوا blue + +148 +00:10:50,200 --> 00:10:56,060 +light عنده مقدر على قتل البكتيريا و لا يؤذي + +149 +00:10:56,060 --> 00:11:00,480 +الإنسان فاعتقد إن هذه التقنية هتحلل مشاكل كبيرة في + +150 +00:11:00,480 --> 00:11:03,720 +ال infection control في المستشفى لسه ما شاعت + +151 +00:11:08,870 --> 00:11:12,630 +طيب ال X-ray احنا قولنا ال X-ray عبارة عن high + +152 +00:11:12,630 --> 00:11:16,730 +energy radiation + +153 +00:11:16,730 --> 00:11:22,870 +that can have penetration power أكتر من اللي هو ال + +154 +00:11:22,870 --> 00:11:26,430 +UV و بتقتل البكتيريا عن طريق إنتاج لـ free + +155 +00:11:26,430 --> 00:11:30,510 +radicals ليش عن ال free radicals؟ للشقوق الحرة ال + +156 +00:11:30,510 --> 00:11:36,360 +H و ال OHالان ال H و ال OH لما بيرتبطوا مع جزئية + +157 +00:11:36,360 --> 00:11:40,740 +الماء او OH بيرتبط مع OH بايش بيطلعوا؟ H2O2 يعني + +158 +00:11:40,740 --> 00:11:44,240 +hydrogen peroxide و كل مرة بداش نعيد نفس الاسطوانة + +159 +00:11:44,240 --> 00:11:48,920 +و نقولكوا ان ال H2O2 هو a very powerful oxidizing + +160 +00:11:48,920 --> 00:11:52,200 +agent و أخدناه من ضمن ال disinfectants من ضمن ال + +161 +00:11:52,200 --> 00:11:54,760 +chemical disinfectants اللي بيأثروا على ال protein + +162 +00:11:54,760 --> 00:12:01,540 +و على ال DNA الان كمان مرة ال spores + +163 +00:12:06,550 --> 00:12:11,510 +مثل اثران من ال vegetative cells بال X-ray قد يعود + +164 +00:12:11,510 --> 00:12:15,690 +السبب في ذلك إلى عدم احتواء ال spores الماء، طبعا + +165 +00:12:15,690 --> 00:12:20,190 +ماهي علاقة الماء في الموضوع؟ الماء من ال .. ومش هو + +166 +00:12:20,190 --> 00:12:25,690 +المنتج لمن؟ الشقوق الحرة قلنا؟ ال H2 ال OH والH + +167 +00:12:25,690 --> 00:12:29,830 +جايين من الماء فبالتالي مافيش ماء يجيب ومافيش شقوق + +168 +00:12:29,830 --> 00:12:34,280 +حرة كافية لقتل اللي هو ال sporesفي معلومة كتير + +169 +00:12:34,280 --> 00:12:37,340 +كويسة بتقول ان الـ sulfhydryl containing compounds + +170 +00:12:37,340 --> 00:12:40,000 +such as amino acids cysteine can protect DNA from + +171 +00:12:40,000 --> 00:12:46,520 +free radical attacks وجود بروتينات تحتوي على + +172 +00:12:46,520 --> 00:12:51,760 +cysteine او او amino acids تحتوي على sulfhydryl + +173 +00:12:51,760 --> 00:12:58,360 +group بيساعد ال DNA في الحماية من مين؟ من ال X-ray + +174 +00:12:58,360 --> 00:13:02,920 +هذا الظهر هو اللي شفوهاشفوها فيه انه فيه بكتيريا + +175 +00:13:02,920 --> 00:13:06,760 +اسمها radio .. radio .. radio bacteria هذه الظاهرة + +176 +00:13:06,760 --> 00:13:12,560 +.. هذه الظاهرة لما درسوا ال .. ال .. التركيب تبع + +177 +00:13:12,560 --> 00:13:16,780 +الخلية وردوا انه بالفعل فيها plenty of self .. + +178 +00:13:16,780 --> 00:13:19,960 +self-hydrating containing اللي هو amino acids + +179 +00:13:22,980 --> 00:13:29,200 +في قلية أخرى لل killing بال X-Ray وهو تكسير ال + +180 +00:13:29,200 --> 00:13:31,700 +covalent bonds في ال DNA نفسه يعني ال DNA نفسه + +181 +00:13:31,700 --> 00:13:36,920 +بتكسر من ال high energy اللي طالعة من ال X-Ray لكن + +182 +00:13:36,920 --> 00:13:41,520 +بقول إن القلية دي أقل أهمية أيضا من القلية رقم 1 + +183 +00:13:41,520 --> 00:13:47,180 +اللي هي عبر ال free radicals ال X-Ray ممكن تستخدم + +184 +00:13:47,180 --> 00:13:52,150 +في الطب في تعقيم ال heat sensitive itemsHeat + +185 +00:13:52,150 --> 00:13:55,770 +sensitive items يعني مركبات أو مكونات تتأثر بمين + +186 +00:13:55,770 --> 00:13:58,970 +بالحرارة، كلمة تتأثر بالحرارة يعني بصير لها damage + +187 +00:13:58,970 --> 00:14:02,330 +بالحرارة، such as sutures بتوع الخيوط اللي + +188 +00:14:02,330 --> 00:14:06,970 +بيستخدموها في تغريز الجراحية and surgical gloves + +189 +00:14:06,970 --> 00:14:11,330 +اللي هي الجوانتي الجراحية المعاقبة أيضا and + +190 +00:14:11,330 --> 00:14:14,570 +plastic items such as syringes، بس بدكن تتذكروا + +191 +00:14:14,570 --> 00:14:22,060 +شغلةإنه clean first التعقيم زمان كان مش مشكلة إنه + +192 +00:14:22,060 --> 00:14:26,000 +يكون الأشياء مش نظيفة ما أحنا بحنعقن لكن لما + +193 +00:14:26,000 --> 00:14:29,700 +اكتشفنا إنه ال remains .. ال remains يعني بقايا + +194 +00:14:29,700 --> 00:14:34,360 +البكتيريا الميتة أو الفطريقة الميتة قد تسبب مرض + +195 +00:14:34,360 --> 00:14:37,660 +للإنسان زي ال endotoxin زي ال libotic acid زي + +196 +00:14:37,660 --> 00:14:41,180 +الbiptidoglycan بعض ال toxins الأخرى اللي سميناها + +197 +00:14:41,180 --> 00:14:47,430 +pyrogens فبتالي كنا clean firstالنوع التالت أو صنف + +198 +00:14:47,430 --> 00:14:49,730 +التالت من ال .. من ال physical agent اللي هو ال + +199 +00:14:49,730 --> 00:14:53,250 +filtration و ال filtration is a mechanical removal + +200 +00:14:53,250 --> 00:14:59,390 +of microorganism by passing through a membrane + +201 +00:14:59,390 --> 00:15:06,290 +filter، membrane filter يعني منقي أو مرشح مكون من + +202 +00:15:06,290 --> 00:15:13,290 +غلاف أو من غشاق بسيط، رقيق، المسامات تبعته ما + +203 +00:15:13,290 --> 00:15:17,170 +بتفوض البكتيريا أو تشتل بأي تانيابتعب الكهربية + +204 +00:15:17,170 --> 00:15:21,150 +الساكنة .. الكهربية الساكنة قد تجعل الأشياء + +205 +00:15:21,150 --> 00:15:27,130 +الصغيرة تلتثق ب .. ببعضها البعض فممكن يكون فيروسات + +206 +00:15:27,130 --> 00:15:31,110 +حجمها أصغر من ال .. من ال poor size تبع ال .. ال + +207 +00:15:31,110 --> 00:15:34,510 +membrane لكن ما تمرش عبره لإنه التثقت بعبر + +208 +00:15:34,510 --> 00:15:38,390 +الكهربية الساكنة بال membrane ال poor size صغير + +209 +00:15:38,390 --> 00:15:42,030 +بيحدث و في نفس الوقت في خاصية ما قلنا كمان مراياش + +210 +00:15:42,030 --> 00:15:47,100 +اللي هي ال electrostatic attractionمن أشهر ال .. + +211 +00:15:47,100 --> 00:15:49,460 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +212 +00:15:49,460 --> 00:15:49,880 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +213 +00:15:49,880 --> 00:15:49,920 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +214 +00:15:49,920 --> 00:15:50,300 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +215 +00:15:50,300 --> 00:15:52,520 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +216 +00:15:52,520 --> 00:15:57,640 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +217 +00:15:57,640 --> 00:15:58,740 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +218 +00:15:58,740 --> 00:16:01,700 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +219 +00:16:01,700 --> 00:16:01,700 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +220 +00:16:01,700 --> 00:16:01,700 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +221 +00:16:01,700 --> 00:16:01,700 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +222 +00:16:01,700 --> 00:16:01,700 +.. ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال .. + +223 +00:16:01,700 --> 00:16:11,000 +ال .. ال .. ال .. ال .. ال .. ال .. ال .. ال + +224 +00:16:15,040 --> 00:16:40,700 +خلّينا نحاول نرسم جهاز صغير تخيلوا + +225 +00:16:40,700 --> 00:16:42,700 +معي هنا مفتوح من الطرفين + +226 +00:16:45,530 --> 00:16:50,350 +جالس على كأس برضه مفتوح لكنه قاعدة بنحط عليها ال + +227 +00:16:50,350 --> 00:16:53,850 +filter تمام؟ + +228 +00:16:53,850 --> 00:16:59,450 +الآن طبعا بنشبكهم مع بعض بطريقة معينة إذا بمغناطيس + +229 +00:16:59,450 --> 00:17:03,130 +أو بمشبك فبنكونوا ركبين فوق بعض و غير منافذين + +230 +00:17:03,130 --> 00:17:09,910 +للماء تمام؟ بنصب عية الماء مثلا 100 مل عبر ال + +231 +00:17:09,910 --> 00:17:14,120 +filter و لأن المسامات صغيرة أجدوا ستنهساعة، + +232 +00:17:14,120 --> 00:17:18,580 +ساعتين، يمكن طول النهار ماينزلش عشرة ملي فمضطرين + +233 +00:17:18,580 --> 00:17:27,540 +احنا نعمل عملية خلخل الهواء هنا عبر vacuum شفت + +234 +00:17:27,540 --> 00:17:31,820 +خلخل الهواء عبر عملية اللي هو التفريغ فالسائل + +235 +00:17:31,820 --> 00:17:37,760 +بيضطر ينزل الآن هنا أي بكتيريا كانت مورودة في + +236 +00:17:37,760 --> 00:17:44,460 +المواصل تلاني على ال membrane filterبنفكر الغطاء + +237 +00:17:44,460 --> 00:17:48,060 +وبناخد ال membrane بملقة معقمة وبنحطه على طبق + +238 +00:17:48,060 --> 00:17:54,420 +تمام؟ وين فيه بكتيريا هتكون مستعمرة كل مستعمرة انا + +239 +00:17:54,420 --> 00:17:57,620 +افترضت انها نشعت من مين؟ من خلية واحدة وبالتالي + +240 +00:17:57,620 --> 00:18:03,600 +انا بقدر اعد و بقول كان فيه 250 مستعمرة او وحدة + +241 +00:18:03,600 --> 00:18:08,320 +بناء مستعمرة ايش سميناها هم؟ هم مين؟ Hormin Unit + +242 +00:18:08,320 --> 00:18:14,330 +وحدة بناء مستعمرة في كل 100 MLمن اللي هو العين هذا + +243 +00:18:14,330 --> 00:18:20,770 +فحص معتبر وبيعتمد عليه جزء كبير من مختبرات الصحة + +244 +00:18:20,770 --> 00:18:24,250 +العامة مختبرات الصحة العامة في منها قسم بس بيكون + +245 +00:18:24,250 --> 00:18:29,630 +بيرتجلوها لميها الشرب بيجي عينات من كل قطاع غزة من + +246 +00:18:29,630 --> 00:18:34,430 +كل بلدية قطاع غزة مرتين تقريبا في الشهر وبيفحصها + +247 +00:18:34,430 --> 00:18:38,520 +عشان يشوف عدد البكتيريا اللي موجودة فيهاصالح للشرب + +248 +00:18:38,520 --> 00:18:41,940 +و لا غير صالح للشرب طبعا إذا كان فيه بكتيريا من + +249 +00:18:41,940 --> 00:18:46,780 +نوع Coliform فهو يكون غير صالح للشرب الفكرة + +250 +00:18:46,780 --> 00:18:49,500 +التانية اللي بنشتغلها و بنستخدم فيها ال filtration + +251 +00:18:49,500 --> 00:18:54,940 +لما طالباتنا في البحث أو طلالنا في البحث بيشتغلوا + +252 +00:18:54,940 --> 00:19:00,340 +مركب و بيدموا يشوفوا هذا المركب مدى قدرته على قتل + +253 +00:19:00,340 --> 00:19:03,160 +الميكروبات احنا خايفين ان المركب أساسا هم بيشتغلوا + +254 +00:19:03,160 --> 00:19:07,670 +فيه بيحضروا فيه يكون ملورطب نروح بالداخل و عبر + +255 +00:19:07,670 --> 00:19:10,410 +membrane filter بس المرة هذه بطريقة مختلفة شوية + +256 +00:19:10,410 --> 00:19:19,670 +بتاعة فوق ال .. السيرنجة هاد ال needle و على الطرف + +257 +00:19:19,670 --> 00:19:27,850 +التاني هناك بيكون سيرنج ال + +258 +00:19:27,850 --> 00:19:32,150 +membrane filter بيكون في plastic container مفتوح + +259 +00:19:32,150 --> 00:19:36,440 +للطرفين طرف بيلاسق فيه ال needleوطلب من اللذك فيه + +260 +00:19:36,440 --> 00:19:39,320 +اللي هو الـ syringe الان هي المحلول تبعنا هنا في + +261 +00:19:39,320 --> 00:19:41,760 +المنطقة هذه طبعا كلكم بتعرفوا أن الـ syringe + +262 +00:19:41,760 --> 00:19:46,040 +بينفكها و ال needle هي تكون معقّمة لكن ال needle + +263 +00:19:46,040 --> 00:19:51,200 +دخلت في ميعاق غير معقّمة بس الـ syringe مُعقّمة + +264 +00:19:51,200 --> 00:19:55,800 +ايه الفاصل بينهم؟ ال membrane filter سحبنا السائل، + +265 +00:19:55,800 --> 00:19:59,680 +السائل مر قبل ال filter كل البكتيريا حُجزت فالان + +266 +00:19:59,680 --> 00:20:04,460 +اللي موجود في الـ syringeالمرة دي سافرت ل .. سافرت + +267 +00:20:04,460 --> 00:20:08,980 +لإيش؟ للتعقيد لو بدي أعمل urea solution وانتوا + +268 +00:20:08,980 --> 00:20:12,780 +قعدتوا .. قعدتوا في المختبر درستوا ال .. ال + +269 +00:20:12,780 --> 00:20:15,260 +biochemical test و أحد ال biochemical test ال + +270 +00:20:15,260 --> 00:20:19,340 +urease ال urease فحص معتمد على إنه الإنزين بيكسر + +271 +00:20:19,340 --> 00:20:24,200 +urea أو بيكسرهاش ال urea لو حطيناها بال autoclave + +272 +00:20:24,200 --> 00:20:28,280 +بتتكسر لحالها it's sensitive material فهنضطر لما + +273 +00:20:28,280 --> 00:20:32,140 +نحضركوا ال media ان أعقملكوا ال urea عبر مين + +274 +00:20:32,430 --> 00:20:37,370 +الفيلتر يمكن استخدامه كتستنج او كعملية + +275 +00:20:43,790 --> 00:20:49,150 +في ختم المحاضرة بدنا نذكر أهم النقاط اللي ذكرت في + +276 +00:20:49,150 --> 00:20:52,230 +المحاضرة كلمة sterilization و disinfection ميزنا + +277 +00:20:52,230 --> 00:20:55,650 +بينهم تمييز دقيق absolute والتانية مش absolute + +278 +00:20:55,650 --> 00:20:59,930 +التانية بتقولنا إيه؟ بتقولنا اللي هي disinfection + +279 +00:20:59,930 --> 00:21:02,850 +بتقولنا إنه reducing the number إيش يعني reducing + +280 +00:21:02,850 --> 00:21:07,820 +the number؟تقليل العدد بحيث ان ال infection is not + +281 +00:21:07,820 --> 00:21:10,480 +likely to occur، ايش يعني is not likely to occur؟ + +282 +00:21:10,480 --> 00:21:13,100 +يعني ما يصير .. في احتمالية حدوث ال infection + +283 +00:21:13,100 --> 00:21:16,400 +بتكون قليلة، احتمالية حدوث ال infection بتكون + +284 +00:21:16,400 --> 00:21:19,840 +قليلة، هنا في فرق ما بين ال .. ال sterilization و + +285 +00:21:19,840 --> 00:21:24,780 +ايه؟ ال disinfectionاتكلمنا عن نوعين من ال .. ال + +286 +00:21:24,780 --> 00:21:27,560 +.. ال disinfectants، واحد سمينا antiseptic، واحد + +287 +00:21:27,560 --> 00:21:31,320 +سمينا disinfectant بحسب اللي هو الاستخدام، واشهر + +288 +00:21:31,320 --> 00:21:35,540 +واحد كان اللي هو ال 70% Ethanol، وعدتنين بيشتغل، + +289 +00:21:35,540 --> 00:21:39,200 +على ال .. in animate object أو على اللي هو ال .. + +290 +00:21:39,200 --> 00:21:44,420 +ال animate objectالـ Killing للميكروبات بيكون + +291 +00:21:44,420 --> 00:21:48,480 +proportional ل ال concentration اللي هي ال + +292 +00:21:48,480 --> 00:21:51,180 +concentration والطعم ايش سميناهم؟ ال concentration + +293 +00:21:51,180 --> 00:21:57,380 +والطعم مع بعض سميناهم ال dos اللي هي الجراس اتفقنا + +294 +00:21:57,380 --> 00:22:02,020 +على انه كمان القتل من قبل ال chemical بينبغي تحت + +295 +00:22:02,020 --> 00:22:05,580 +واحد من تلت ميكانزمات الأولى cell membrane limits + +296 +00:22:05,580 --> 00:22:10,460 +protein modification DNA modification + +297 +00:22:26,960 --> 00:22:30,840 +الوحدة الأخيرة اللي قدامكم هي good summary لكل + +298 +00:22:30,840 --> 00:22:33,200 +الأشياء اللي درسناها ونكمل ان شاء الله المرة + +299 +00:22:33,200 --> 00:22:33,540 +القادمة + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/xg8iBc9OMFs_postprocess.srt b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/xg8iBc9OMFs_postprocess.srt new file mode 100644 index 0000000000000000000000000000000000000000..2f12ece9b0e2d2f8aeb217cf75f8f1bb0e009a2d --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/xg8iBc9OMFs_postprocess.srt @@ -0,0 +1,2444 @@ +1 +00:00:21,190 --> 00:00:22,970 +بسم الله الرحمن الرحيم الحمد لله وبرحمة الله + +2 +00:00:22,970 --> 00:00:24,130 +وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله + +3 +00:00:24,130 --> 00:00:24,950 +وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله + +4 +00:00:24,950 --> 00:00:25,610 +وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله + +5 +00:00:25,610 --> 00:00:27,190 +وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله + +6 +00:00:27,190 --> 00:00:35,790 +وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة + +7 +00:00:35,790 --> 00:00:42,420 +الله وبرحمة الله وبرحمة اللهال food poisoning بشكل + +8 +00:00:42,420 --> 00:00:45,460 +عام وال infection وال salmonella وفي كان موضوع + +9 +00:00:45,460 --> 00:00:51,380 +بتكلم عن ال botulism وإيش كان موضوع ال .. الكفين + +10 +00:00:51,380 --> 00:00:55,920 +برضه أكل برضه شرب يعني فاليوم مقعدانا كلها متعلقة + +11 +00:00:55,920 --> 00:00:58,440 +في هذا اللي ضال وحتى تصوروا سبسنة اللي هو علاقة + +12 +00:00:58,440 --> 00:01:03,800 +بيه إلك السلام عليكم أنا طالبة دوعسي حوال اليوم + +13 +00:01:03,800 --> 00:01:08,200 +موضوعنا راح يكون عن pseudomonas botida المسمى + +14 +00:01:08,200 --> 00:01:14,700 +بكتير الكفاينيعود اكتشاف هذه البكتيريا إلى ال E + +15 +00:01:14,700 --> 00:01:20,640 +.coli منذ زمن بعيد قام بعض العلماء بهندسة لل E + +16 +00:01:20,640 --> 00:01:25,900 +.coli بيجعلها تضمن على الكوفين واستخدموها في إزالة + +17 +00:01:25,900 --> 00:01:29,480 +ال contamination من ال waste water وايضا استخدموها + +18 +00:01:29,480 --> 00:01:33,180 +في ال treatment للأزمة و ال chronic lung disease + +19 +00:01:34,900 --> 00:01:40,240 +وفي عام 2011 تم اكتشاف بكتيريا مضمنة على الكوفيين + +20 +00:01:40,240 --> 00:01:45,140 +لكن ليست هي ال E.coli التي تم هندستها منذ زمن بعيد + +21 +00:01:45,140 --> 00:01:51,480 +وفي عام 2013 قاموا بنقل جين ال pseudomonas putida + +22 +00:01:51,480 --> 00:01:56,420 +إلى ال E.coli لجعلها تضمن على الكوفيين وذلك يعود + +23 +00:01:56,420 --> 00:02:02,500 +لأن ال E.coli هي العمود الفقري للبيولوجيا الحيويةو + +24 +00:02:02,500 --> 00:02:07,940 +ذلك يعود لأنها سريعة التضاعف وسهلة التعامل في + +25 +00:02:07,940 --> 00:02:14,160 +المختبر هذه البكتيريا تتميز ب characteristic بأنها + +26 +00:02:14,160 --> 00:02:18,020 +normal microbiota in soil أي أنها flora في التربة + +27 +00:02:18,020 --> 00:02:23,460 +وهي gram negative basilii فهي تمتلك long rod shape + +28 +00:02:23,460 --> 00:02:27,760 +كما هو مبين في ال slides وهي إما تمتلك one + +29 +00:02:27,760 --> 00:02:32,050 +flagellum or more flagellumوأيضا هي non + +30 +00:02:32,050 --> 00:02:36,610 +-dysporiformic هي aerobic هي mesophilic أي أنها ال + +31 +00:02:36,610 --> 00:02:41,790 +optimal growth لديها ما بين 25 إلى 37 درجة سيليزيا + +32 +00:02:41,790 --> 00:02:47,910 +وهي chemotheriotropic, fluorescent and oxytase + +33 +00:02:47,910 --> 00:02:54,350 +positive ويوجد لهذه البكتيريا other strains غير + +34 +00:02:54,350 --> 00:03:01,070 +الكفالينثيوبرومين وبراجسانثين ومثالي براجسانثين + +35 +00:03:01,070 --> 00:03:06,210 +وهو ثيوفيلين وكل من هذه المركبات يشكل نسبة من + +36 +00:03:06,210 --> 00:03:12,690 +الكفاين فالبراجسانثين يشكل 84% وثيوبرومين 12% + +37 +00:03:12,690 --> 00:03:20,310 +وثيوفيلين 4% ويوجد لكل من هذه المركبات + +38 +00:03:20,310 --> 00:03:27,820 +مميزات تميزها عنغيرها فان فإن البرفسانتيز يزود + +39 +00:03:27,820 --> 00:03:32,500 +الليبولايسيز والجيوسرول و ال free fatty acid في ال + +40 +00:03:32,500 --> 00:03:36,900 +blood plasma وثيوبرومين بيعمل ديالاك ل ال blood .. + +41 +00:03:36,900 --> 00:03:40,260 +لل blood vessels and decrease urine volume و أيضا + +42 +00:03:40,260 --> 00:03:47,410 +هذا يوجد في العند الكوكابين وهو حبات بون الكاكاو + +43 +00:03:47,410 --> 00:03:52,450 +والثيوفيلين الذي هو عبارة عن 7-methylxanthine + +44 +00:03:52,450 --> 00:03:56,830 +بيعمل releasing relaxation ل ال smooth muscles و + +45 +00:03:56,830 --> 00:04:03,830 +يستخدم في الترابي للأزيمةوهذا احدى مسارات تصنيع + +46 +00:04:03,830 --> 00:04:08,930 +الكفايين، تصنيع الكفايين لديه تلت مسارات، هذا واحد + +47 +00:04:08,930 --> 00:04:16,510 +منهم، بداية يتم تحويل 7-methyl-hexanothene إلى un + +48 +00:04:16,510 --> 00:04:20,150 +-methyl، إلى ثيوبرومين عن طريق الـun-methyl + +49 +00:04:20,150 --> 00:04:24,250 +-nucleidase ومن ثم يتم تصنيع الكفايين، اللذي هو + +50 +00:04:24,250 --> 00:04:28,910 +عبارة عن 137-triethylhexanothene + +51 +00:04:31,250 --> 00:04:37,330 +هذه البكتيريا يمكن لها أن تعيش في بكتيريا في وسط + +52 +00:04:37,330 --> 00:04:42,250 +فقط يوجد فيه الكفايين، وتقسر الكفايين إلى carbon + +53 +00:04:42,250 --> 00:04:46,290 +dioxide و أمونيا، ال carbon dioxide كمصدر للكربون + +54 +00:04:46,290 --> 00:04:52,870 +والأمونيا كمصدر للنيكروجينو هذه البكتيريا المصدر + +55 +00:04:52,870 --> 00:04:58,450 +الوحيد لها لل carbon و ال nitrogen هو الكفايين و + +56 +00:04:58,450 --> 00:05:01,030 +ال carbon و ال nitrogen من المركبات الأساسية + +57 +00:05:01,030 --> 00:05:05,530 +لتصنيع أي خلية و من ضمنها الخلية البكتيرية لدى + +58 +00:05:05,530 --> 00:05:10,290 +البكتيريا Sodomonas Botida و هذه البكتيريا يمكن + +59 +00:05:10,290 --> 00:05:15,870 +تمييتها في ال enrichment cultureالتي هي تمتلك + +60 +00:05:15,870 --> 00:05:21,090 +essential growth factor ويقول الباحثين أن هذه + +61 +00:05:21,090 --> 00:05:24,730 +البكتيريا سيتعدى استخدامها في ال environmental + +62 +00:05:24,730 --> 00:05:30,710 +remendations أي في المعالجة البيئيةمن استخدامات + +63 +00:05:30,710 --> 00:05:35,950 +هذه البكتيريا تستخدم cavital sensors or biosensors + +64 +00:05:35,950 --> 00:05:42,770 +هذا المصطلح يعني قياس مستوى أي شيء أو شيء معين في + +65 +00:05:42,770 --> 00:05:47,870 +ال bioscience فهي تستخدم لقياس الكفايين في ال + +66 +00:05:47,870 --> 00:05:50,250 +privilege اللي هي المشروبات اللتي تحتوي على + +67 +00:05:50,250 --> 00:05:53,850 +الكفايين و لقياس أيضا ال contamination area + +68 +00:05:53,850 --> 00:05:57,730 +المناطق الملوثة بالكفايين و أيضا نستخدم هذه + +69 +00:05:57,730 --> 00:06:02,260 +البكتيريا فيDetoxification لبعض الـ Candery Output + +70 +00:06:02,260 --> 00:06:05,920 +لتصنيع الكفيه و أيضا تستخدم في ال bio production + +71 +00:06:05,920 --> 00:06:09,460 +للصم الثيرابيث مثل ال asthma و ال pulmonary + +72 +00:06:09,460 --> 00:06:16,180 +disease وتستخدم لتقوم بتحفيز ال ethanol على تصنيع + +73 +00:06:16,180 --> 00:06:22,180 +ال bio fuel وهو الوقود الإحفوريولو جينا نتصور ما + +74 +00:06:22,180 --> 00:06:26,900 +لهذه البكتيريا من فائدة في إزالة العديد من ملوثات + +75 +00:06:26,900 --> 00:06:30,920 +الكافيين الذي أكد البحر تون على أن أكثر ملوثات + +76 +00:06:30,920 --> 00:06:35,630 +المياه العادمة هو مخلفات الكافيينفلو جينا ننظر من + +77 +00:06:35,630 --> 00:06:41,010 +زاوية صغيرة على كل شخص مننا كم يخلف في اليوم من + +78 +00:06:41,010 --> 00:06:45,410 +مخلفات الكفاين فالكفاين موجود في العديد من + +79 +00:06:45,410 --> 00:06:48,630 +المشروبات و العديد من الأطعمة و أيضا في بعض + +80 +00:06:48,630 --> 00:06:53,310 +الأدوية فهو موجود بالنسبة للمشروبات في الشاي في + +81 +00:06:53,310 --> 00:06:57,130 +القهوة في الناس كافة في المشروبات الطاقة و أيضا في + +82 +00:06:57,130 --> 00:07:00,410 +المشروبات السودا فمن منا لا يتناول خلال اليوم شيء + +83 +00:07:00,410 --> 00:07:04,360 +من هذه المشروباتو أيضا توجد في الأطعمة مثل ال + +84 +00:07:04,360 --> 00:07:08,300 +chocolate و غيرها و بالنسبة لل therapy فهي موجودة + +85 +00:07:08,300 --> 00:07:15,460 +في جميع أدويات ال pulmonary disease فلو جينا نتصور + +86 +00:07:15,460 --> 00:07:20,440 +مال هذه البكتيريا من فائدة في إزالة أكبر عبق في + +87 +00:07:20,440 --> 00:07:26,810 +المياه العادمة فسيكون لها فائدة كبيرة و شكرابسم + +88 +00:07:26,810 --> 00:07:29,450 +الله الرحمن الرحيم وصلاة و سلام على أشهر الإنسانين + +89 +00:07:29,450 --> 00:07:31,730 +أنا طالب السجن الجودر و روح أقدملكم اليوم باسم + +90 +00:07:31,730 --> 00:07:33,470 +الله presentation عن البطوليزم + +91 +00:07:37,140 --> 00:07:46,500 +البوتيوليزم هو عبارة + +92 +00:07:46,500 --> 00:07:49,680 +عن مرض يؤدي إلى ارتخاء العضلات بسبب نيروتوكسين + +93 +00:07:49,680 --> 00:07:52,980 +يسمى البوتيلونيوم توكسين الذي يسبب بوتيوليزم + +94 +00:07:52,980 --> 00:07:57,060 +الكلسردينبوتيلونيوم هو المسبب للبوتيوليزم وعنا + +95 +00:07:57,060 --> 00:08:00,360 +صورة بتوضح الكلسردينبوتيلونيوم اللى هنقض على بعض + +96 +00:08:00,360 --> 00:08:02,920 +الخصائص عند كلسردينبوتيلونيوم + +97 +00:08:04,360 --> 00:08:06,740 +الكلستراديوميتالونيوم is the name of bacteria + +98 +00:08:06,740 --> 00:08:09,540 +camouflified in soil and has and have a + +99 +00:08:09,540 --> 00:08:12,760 +characteristic anaerobic, gram-positive, rodent + +100 +00:08:12,760 --> 00:08:18,020 +-shell bacteria of form sparHow Clostridium + +101 +00:08:18,020 --> 00:08:21,380 +Botulinum cause Botulism لنيروتوكسين Synthesis N + +102 +00:08:21,380 --> 00:08:24,440 +secreted by Clostridium Botulinum bacteria cause + +103 +00:08:24,440 --> 00:08:28,000 +Botulism. This toxin blocking الacetylcholine for + +104 +00:08:28,000 --> 00:08:31,860 +motor nerve ending أي أن التوكسين الذي يكون + +105 +00:08:31,860 --> 00:08:34,940 +بإفرازه Clostridium Botulinum تعمل إيقاف لعمل + +106 +00:08:34,940 --> 00:08:38,220 +نيروتوكسين ومن المعروف أن نيروتوكسين عبارة عن نقل + +107 +00:08:38,220 --> 00:08:42,080 +عصبي فتعمل إيقاف للacetylcholine وهتظهر أن بعض + +108 +00:08:42,080 --> 00:08:46,350 +السيمبتوزم الان هتأخذهاعنا أنواع لإيوتيوليزم، فى + +109 +00:08:46,350 --> 00:08:48,630 +عندنا ال foodborne petulism، فى عندنا ال want + +110 +00:08:48,630 --> 00:08:52,230 +petulism و فى عندنا ال infant petulism، بالنسبة لل + +111 +00:08:52,230 --> 00:08:55,270 +foodborne petulism، النوع من أنواع ال petulism + +112 +00:08:55,270 --> 00:08:59,550 +يحدث عنده مثلا هو البشر الإنسان وشبة مليئة بال .. + +113 +00:08:59,550 --> 00:09:03,600 +بالنيروتوكسين اللى يسببه الوكلسرد والمتلونينأول + +114 +00:09:03,600 --> 00:09:07,740 +ظهور كان لهذا النوع من المرض في كندا لما صار في + +115 +00:09:07,740 --> 00:09:11,540 +عنا تخمر في بعض أنواع العلب السمك و ايضا في نيو + +116 +00:09:11,540 --> 00:09:15,740 +يورك لما صار في عنا فساد لكمية كبيرة من التوفويا + +117 +00:09:15,740 --> 00:09:21,200 +نوع من أنواع الجبنة هذه الصورة بتبين ان بعد ما + +118 +00:09:21,200 --> 00:09:24,600 +الانسان تناول واجبة من محتوي على النيو يوركتوكسين + +119 +00:09:24,600 --> 00:09:28,740 +اللي ده سببه الكلسترود المتلونيوم و تبين استقرار + +120 +00:09:28,740 --> 00:09:30,960 +النوع من أنواع البكتيريا في المعدة + +121 +00:09:33,970 --> 00:09:37,150 +الـ Wound Botulism is caused by neurotoxin + +122 +00:09:37,150 --> 00:09:40,330 +produced within a wound that is infected with the + +123 +00:09:40,330 --> 00:09:42,590 +Clostridium Botulorum، أيضا المسبب ليه الـ Wound + +124 +00:09:42,590 --> 00:09:46,770 +Botulism هيكون Clostridium Botulorumأما بالنسبة + +125 +00:09:46,770 --> 00:09:49,690 +لإنفد بوتولوزم a core when infant in consume the + +126 +00:09:49,690 --> 00:09:52,670 +spore مالمعروف انه الانفد معتمد ده فيه ردائها على + +127 +00:09:52,670 --> 00:09:55,550 +الحليب لكن كيف يعني يدخل ال gluceridylmethylamine + +128 +00:09:55,550 --> 00:09:59,210 +الانفد نفسه يقوم باستهلاك الاسبور، هذه الاسبور + +129 +00:09:59,210 --> 00:10:02,370 +هتدخل لإنسان اللي لا الانفد وتصبح دقية وتقوم + +130 +00:10:02,370 --> 00:10:04,790 +بتكوينه من ال neurotoxin اللي هيأثر على + +131 +00:10:04,790 --> 00:10:08,790 +الacetylcholine ويبدأ يشغل بشغل عنا برضه هنا صورة + +132 +00:10:08,790 --> 00:10:13,160 +بتبين لبعض الأعراف من مرض البوتولوزمناخد ال + +133 +00:10:13,160 --> 00:10:15,860 +botulism & symbiotism اول اشي فى عنا ال double + +134 +00:10:15,860 --> 00:10:18,280 +vision فى عنا ال blood vision يعني فى اللى هيأثر + +135 +00:10:18,280 --> 00:10:21,680 +على الرؤية ممكن ان يصبح double ال vision يعني + +136 +00:10:21,680 --> 00:10:24,620 +الصورة اللى قدامه بيشوفها اكثر من صورة سورتين مثلا + +137 +00:10:24,620 --> 00:10:28,180 +او ممكن تشوف الصورة ضبابية او اللى ضربك فى الإيلد + +138 +00:10:28,180 --> 00:10:31,060 +يعني انخفاض شف من العين difficult speech difficult + +139 +00:10:31,060 --> 00:10:34,240 +swallowing صعوبة في التحدث صعوبة في التنفس ال dry + +140 +00:10:34,240 --> 00:10:36,300 +mouth و ال muscles weakness بشكل عام هيصير فى عنا + +141 +00:10:36,300 --> 00:10:38,920 +بقيم احنا حركينا paralysis هصير فى عنا ضعف فيه + +142 +00:10:38,920 --> 00:10:42,120 +العضلاتو كذلك ال constipation ممكن ان الاكولان + +143 +00:10:42,120 --> 00:10:46,000 +مجوللكن ال sympathism ولا ال infant botulism هناك + +144 +00:10:46,000 --> 00:10:51,200 +بعض من ال sympathism تختصب بال infant botulism من + +145 +00:10:51,200 --> 00:10:54,020 +أمثل على ال sympathism هي ال lethargic، ال weak، + +146 +00:10:54,020 --> 00:10:57,580 +ال floppy، ال feed poorly و ال weak cry و ال poor + +147 +00:10:57,580 --> 00:11:00,540 +muscle stone و عادة أول sympathism ممكن يظهر في ال + +148 +00:11:00,540 --> 00:11:03,920 +infant botulism هي ال consumations عادة بيبين على + +149 +00:11:03,920 --> 00:11:06,640 +ال infant بشكل عام البكاء عنده يكون بشكل ضعيف + +150 +00:11:06,640 --> 00:11:10,880 +ارتخاء في العضلات بشكل كامل لل infanthow soon do + +151 +00:11:10,880 --> 00:11:13,580 +sympathism appear؟ in food bomb, spiritual + +152 +00:11:13,580 --> 00:11:17,680 +sympathism عادة تاخد من 18 ل 6 و 3 ساعة في الظهور + +153 +00:11:17,680 --> 00:11:21,120 +لكن ممكن أن المرض يظهر بفترة أكثر من ذلك يعني + +154 +00:11:21,120 --> 00:11:24,060 +بحدود 10 أيام ممكن تظهر و ممكن يكون لنا بفترة أقل + +155 +00:11:24,060 --> 00:11:27,140 +من ذلك يعني حوالي 6 اش لنا ساعة يعني و ذلك بناء + +156 +00:11:27,140 --> 00:11:30,460 +على الواجبة اللي كان يعني اقتراولها و بناء على + +157 +00:11:30,460 --> 00:11:32,760 +الكوليستردوميتالونيوم و النيروتوكسين المحتوية في + +158 +00:11:32,760 --> 00:11:36,970 +الواجبةThese are all symptoms of the muscle + +159 +00:11:36,970 --> 00:11:39,610 +paralysis that is caused by the bacterial notoxin. + +160 +00:11:39,690 --> 00:11:42,750 +If untreated, this symptom may be gross paralysis. + +161 +00:11:42,890 --> 00:11:46,690 +يعني في الحالة اللتي لم يتم فيها العلاج لمرض + +162 +00:11:46,690 --> 00:11:50,130 +البطوليزم، هيصير فعلا paralysis في كل أنحاء الجسم + +163 +00:11:50,130 --> 00:11:53,790 +وفي أن عضلات في جسم الإنسان ممكن تختصب ال + +164 +00:11:53,790 --> 00:11:56,730 +paralysis مثلا ذاك فعلا ممكن يكونوا فعلا ال arms، + +165 +00:11:56,730 --> 00:11:58,890 +ال leg، ال tongue و ال breathing muscles اللي هي + +166 +00:11:58,890 --> 00:12:02,500 +عضلات التنفسهنا عندنا فيهنا صورة بتبين ان شخص بعلم + +167 +00:12:02,500 --> 00:12:05,740 +المرض البرتوليزم و تقريبا يعني شخص يقض عيناه بشكل + +168 +00:12:05,740 --> 00:12:08,820 +كامل اول ما بدأ يعني تقريبا اول سيبتوت ان تظهر + +169 +00:12:08,820 --> 00:12:12,220 +عليه هي ابتخاء لجرفن العين اللي هي ال dropping في + +170 +00:12:12,220 --> 00:12:17,580 +ال a penHow to is botulism diagnosis؟ ال patient + +171 +00:12:17,580 --> 00:12:19,720 +history و ال physical examinations اللى بتظهر على + +172 +00:12:19,720 --> 00:12:23,060 +ال patient ممكن ماتعبرش بشكل كامل عن إنه مريض أو + +173 +00:12:23,060 --> 00:12:25,960 +ممكن لتجزم بإنه مريض و يعانى من ال botulism لأنه + +174 +00:12:25,960 --> 00:12:29,000 +معروف فيه يعني الأعراض و ممكن الأعراض تتشابه مع + +175 +00:12:29,000 --> 00:12:32,180 +أمراض أخرى، لذلك بنتجة في عملية ال diagnosis لسم + +176 +00:12:32,180 --> 00:12:35,220 +ال botulism بال brain scan، بال spinal fluid + +177 +00:12:35,220 --> 00:12:39,630 +examination، ال nerve conductions و other testsبعد + +178 +00:12:39,630 --> 00:12:42,730 +ما عرفنا .. بعد ما عملنا diagnostic لشخص واتأكدنا + +179 +00:12:42,730 --> 00:12:45,510 +ان الشخص بيعني من مرض ابوتيريزم لازم تتعمل ال + +180 +00:12:45,510 --> 00:12:49,210 +treatment أول الشيء ممكن ان ال food poor و ال one + +181 +00:12:49,210 --> 00:12:52,950 +ابوتيريزم تحدثوا عملية ال treatment بال antitoxin + +182 +00:12:52,950 --> 00:12:57,290 +اللي يعمل عملية ال blocking لل neurotoxin و + +183 +00:12:57,290 --> 00:13:05,330 +بالتالي يتوقف ال neurotoxin العمل و يتوقفمن الأنثى + +184 +00:13:05,330 --> 00:13:07,470 +على الانتدوكسين اللى ممكن نستخدمه يالى Trivalent + +185 +00:13:07,470 --> 00:13:10,650 +وLipid Trivalent، هذه الانتدوكسين تُعطى ليه ال + +186 +00:13:10,650 --> 00:13:13,710 +patient بتحفظ عملية فم تجند ال patient، في هذه + +187 +00:13:13,710 --> 00:13:16,370 +الحالة هيتخلص ال patient من ال contamination food + +188 +00:13:16,370 --> 00:13:18,750 +اللى كان بتناولها و اللى بتحتوى على الليورتوكسين، + +189 +00:13:18,750 --> 00:13:21,740 +وفي هذه الحالةبعض انواع للعقاقية في هذه الحالة + +190 +00:13:21,740 --> 00:13:25,600 +يعني ستمشي فيها المريض شكرا In the name of Allah + +191 +00:13:25,600 --> 00:13:27,880 +and mercifully praise be to Allah lord of the + +192 +00:13:27,880 --> 00:13:29,980 +world and peace to be upon our beloved prophet + +193 +00:13:29,980 --> 00:13:32,840 +Muhammad I am Renate Ibrahim Abedin and today I + +194 +00:13:32,840 --> 00:13:35,420 +want to speak about biofilms at the first of my + +195 +00:13:35,420 --> 00:13:37,960 +speech I want to give you a brief definition about + +196 +00:13:37,960 --> 00:13:40,860 +biofilms biofilms are communities of + +197 +00:13:40,860 --> 00:13:43,280 +microorganisms in a matrix that joins them + +198 +00:13:43,280 --> 00:13:45,840 +together into living or inert substrates so + +199 +00:13:45,840 --> 00:13:50,280 +aggregation of microorganisms called biofilmsWhere + +200 +00:13:50,280 --> 00:13:53,380 +we found biofilm؟ Biofilm found in nature. We + +201 +00:13:53,380 --> 00:13:57,180 +found biofilm on teeth as a blaze or submerged on + +202 +00:13:57,180 --> 00:13:59,980 +rocks in a stream. There is a number of pathogens + +203 +00:13:59,980 --> 00:14:02,320 +that have been studied for the formation of + +204 +00:14:02,320 --> 00:14:06,320 +biofilm, cephalococcus aureus, cephalococcus + +205 +00:14:06,320 --> 00:14:09,760 +mutans, salmonella typhi, enterococcus vicaris and + +206 +00:14:09,760 --> 00:14:14,130 +pseudomonas eugenosaالشخصية التي تم اكتشافها الـ + +207 +00:14:14,130 --> 00:14:17,990 +Bi-Film هي أن الـ Submerged Bi-Film يبدو أنه يخلق + +208 +00:14:17,990 --> 00:14:22,030 +مشروعات مثل المشروعات التي يتم تقريرها من خلال + +209 +00:14:22,030 --> 00:14:24,430 +قناعات مليئة بالماء. الـ Floating Bi-Films يخلقون + +210 +00:14:24,430 --> 00:14:27,070 +شكل أو بيليكل اللي يتعامل بالهواء مع التعامل اللي + +211 +00:14:27,070 --> 00:14:28,030 +يتعامل مع التعامل اللي يتعامل مع التعامل اللي + +212 +00:14:28,030 --> 00:14:28,690 +يتعامل مع التعامل اللي يتعامل مع التعامل اللي + +213 +00:14:28,690 --> 00:14:29,390 +يتعامل مع التعامل اللي يتعامل مع التعامل اللي + +214 +00:14:29,390 --> 00:14:32,430 +يتعامل مع التعامل اللي يتعامل مع التعامل اللي + +215 +00:14:32,430 --> 00:14:38,000 +يتعامل مع التعامل اللي يتعالأول هو التواصل أو + +216 +00:14:38,000 --> 00:14:40,960 +الانشطاء من التصميم الفيلم البيعي، التعامل بين + +217 +00:14:40,960 --> 00:14:44,840 +الجلدين مع بعضها أو في الأعلى، ثم التجارب + +218 +00:14:44,840 --> 00:14:49,660 +الفيلمية، ثم تصميم الجلدين لإنشطة إضافية، مصممة + +219 +00:14:49,660 --> 00:14:53,600 +الفيلم البيعي مختلفة جدًا بسبب الانشطاء المحددة في + +220 +00:14:53,600 --> 00:14:59,060 +الفيلم والوضع البيعيالأول هو التواصل، سيكون هناك + +221 +00:14:59,060 --> 00:15:02,440 +التواصل بين الميكروغنسما بين بعضنا البعض أو بين + +222 +00:15:02,440 --> 00:15:07,480 +المجتمع، ثم الميكروغنسما يتواصلون ويصبحون مجموعة + +223 +00:15:07,480 --> 00:15:13,440 +بوليساكل عيدرية أكتر ثم الأخير هو التواصل، التواصل + +224 +00:15:13,440 --> 00:15:16,500 +يعني أن كل جزء من البيوفيلم يتم تجريه من هذا + +225 +00:15:16,500 --> 00:15:19,460 +البيوفيلم ويسبب فيه بيوفيلم جديد في المناطق + +226 +00:15:19,460 --> 00:15:23,550 +الجديدةلقد رأينا قبل ذلك أن البلاي يساعدون في + +227 +00:15:23,550 --> 00:15:27,810 +الانهيزة أو الانهيزة الانهيزة الكونجية كبيرة تسرق + +228 +00:15:27,810 --> 00:15:29,490 +انهيزة الانهيزة الانهيزة الانهيزة الانهيزة + +229 +00:15:29,490 --> 00:15:30,070 +الانهيزة الانهيزة الانهيزة الانهيزة الانهيزة + +230 +00:15:30,070 --> 00:15:31,070 +الانهيزة الانهيزة الانهيزة الانهيزة الانهيزة + +231 +00:15:31,070 --> 00:15:35,490 +الانهيزة الانهيزة + +232 +00:15:36,650 --> 00:15:40,330 +كل شخص لديه لغته الخاصة، هذه الصورة جميلة جدًا + +233 +00:15:40,330 --> 00:15:43,510 +وقالت لنا أن الخلقات تتكلم مع بعضها مع بعضها، لكن + +234 +00:15:43,510 --> 00:15:47,110 +هل هم يتكلمون مثلنا؟ لا، هم يتكلمون مع بعضها مع + +235 +00:15:47,110 --> 00:15:47,750 +بعضها مع بعضها مع بعضها مع بعضها مع بعضها مع بعضها + +236 +00:15:47,750 --> 00:15:50,250 +مع بعضها مع بعضها مع بعضها مع بعضها مع بعضها مع + +237 +00:15:50,250 --> 00:15:50,330 +بعضها مع بعضها مع بعضها مع بعضها مع بعضها مع بعضها + +238 +00:15:50,330 --> 00:15:50,690 +مع بعضها مع بعضها مع بعضها مع بعضها مع بعضها مع + +239 +00:15:50,690 --> 00:15:52,890 +بعضها مع بعضها مع بعضها مع بعضها مع بعضها مع بعضها + +240 +00:15:52,890 --> 00:15:55,710 +مع بعضها مع بعضها مع بعضها مع بعضها مع بعضها مع + +241 +00:15:55,710 --> 00:16:05,770 +بعضها مع بعضها مع بعضها مع بعض + +242 +00:16:06,260 --> 00:16:08,840 +الصدموناس الريجينوزة تم صنع بيوفيلم يسمى + +243 +00:16:08,840 --> 00:16:13,720 +سيستكفيبروسيز بيوفيلم أعطى مقاومة للأنتيباتيكية + +244 +00:16:13,720 --> 00:16:18,320 +سأشرح هذا الموضوع لاحقًا إبصمانية الأنتيباتيكية + +245 +00:16:18,320 --> 00:16:23,720 +لمقاومة الفاجوسيتوسيز كيف يتم إعطاء بيوفيلم مقاومة + +246 +00:16:23,720 --> 00:16:27,940 +للأنتيباتيكيةبيتالاكتميز ينتج بكتيريا تزيين + +247 +00:16:27,940 --> 00:16:30,940 +تنزينات في الوصول للتعامل مع الانتيبايتك التعامل + +248 +00:16:30,940 --> 00:16:32,080 +مع الانتيبايتك التعامل مع الانتيبايتك التعامل مع + +249 +00:16:32,080 --> 00:16:32,880 +الانتيبايتك التعامل مع الانتيبايتك التعامل مع + +250 +00:16:32,880 --> 00:16:34,600 +الانتيبايتك التعامل مع الانتيبايتك التعامل مع + +251 +00:16:34,600 --> 00:16:39,380 +الانتيبايتك التعامل مع الانتيبايتك التعامل مع + +252 +00:16:39,380 --> 00:16:40,900 +الانتيبايتك التعامل مع الانتيبايتك التعامل مع + +253 +00:16:40,900 --> 00:16:42,760 +الانتيبايتك التعامل مع الانتيبايتك التعامل مع + +254 +00:16:42,760 --> 00:16:45,040 +الانتيبايتك التعامل مع الانتيبايتك التعامل مع + +255 +00:16:45,040 --> 00:16:51,260 +الانتيبايت + +256 +00:16:57,010 --> 00:16:59,870 +بسم الله الرحمن الرحيم أنا الطالبة شروق الانتسا + +257 +00:16:59,870 --> 00:17:03,490 +هاردلكم اليوم عن الموضوع هيكون عن البكتيريا اللي + +258 +00:17:03,490 --> 00:17:08,030 +بتصير في مرضى الفشل الكلوي في وحدة الغزيل الكلوي + +259 +00:17:08,030 --> 00:17:15,500 +في المستشفى يعنى الفشل الكلوي هو عبارة عنان بيكون + +260 +00:17:15,500 --> 00:17:21,300 +المريض انه بطل الكلية تعمل بشكل المناسب وبيضطروا + +261 +00:17:21,300 --> 00:17:26,320 +انهم يعملوله غسيل كلة او استبدال لكلة او زرق كلية + +262 +00:17:26,320 --> 00:17:31,700 +ويتم تحدد الفشل الكلوي من الخفاض نسبة المعدل + +263 +00:17:31,700 --> 00:17:35,640 +الترشيح في الدم بيصير يطلع دم في ال .. في ال urine + +264 +00:17:35,640 --> 00:17:41,580 +او انه ب .. او انه بيبطل الشخص انه يروح يعني انه + +265 +00:17:41,580 --> 00:17:42,380 +على ال .. + +266 +00:17:45,300 --> 00:17:48,380 +بتصير عملية العلاج انه لما يصير عندهم .. يعني + +267 +00:17:48,380 --> 00:17:52,280 +يعملوله غسيل كلوي عن طريق انه بيركبوا يعني + +268 +00:17:52,280 --> 00:17:56,380 +البوبتان في الجسم في ال .. اما في الرقبة او في ال + +269 +00:17:56,380 --> 00:18:00,840 +central line للجسم وبتسمع عليها تصفية الفيات من + +270 +00:18:00,840 --> 00:18:04,800 +الدم عن طريق الجهة السنية، فهذه بعض الصور مش خاصة + +271 +00:18:04,800 --> 00:18:05,280 +في غسلها + +272 +00:18:09,620 --> 00:18:12,160 +Istaphylococcus aureus هي المكونات المنقوضية + +273 +00:18:12,160 --> 00:18:16,160 +الذهبية اللى بتصيب ال .. الاشخاص هدولة من قلة + +274 +00:18:16,160 --> 00:18:20,140 +الاهتمام اللى موجودة بالمستشفيات هى gram positive + +275 +00:18:20,140 --> 00:18:23,100 +coccal bacteria, facultative anaerobic, large + +276 +00:18:23,100 --> 00:18:25,980 +yellow colony or rash media, antibiotic + +277 +00:18:25,980 --> 00:18:30,380 +resistance, bacteriological culture of the nose + +278 +00:18:30,380 --> 00:18:34,480 +and skin as a normal flora هى بتكون موجودة كnormal + +279 +00:18:34,480 --> 00:18:40,020 +flora فى الجسم و تم عزلها من ..التانية ما عزلها من + +280 +00:18:40,020 --> 00:18:44,240 +قنوف العملين في المستشفيات و هي اللي تم الكشف عنها + +281 +00:18:44,240 --> 00:18:46,880 +في هذه ال .. ال media بال birth marker medium + +282 +00:18:46,880 --> 00:18:53,380 +لتربتكاز ساي و بال brain heart infusion توليدين + +283 +00:18:53,380 --> 00:18:59,160 +blue agar تربتفان yeast extract agar وهي بعض الصور + +284 +00:18:59,160 --> 00:19:00,500 +الأطباق اللي تم الكشف عنها في ال + +285 +00:19:06,520 --> 00:19:10,900 +Cephalococcus aureus هي عبارة عن ال .. انها منتشرة + +286 +00:19:10,900 --> 00:19:13,840 +بالأنوف المرضى .. الأنوف العاملين في التقام الطب + +287 +00:19:13,840 --> 00:19:17,720 +في شمال .. في شمال الطرف الغربية تم الكشف عنها + +288 +00:19:17,720 --> 00:19:21,720 +وكانت موجودة على المعاطف ال .. معاطف العاملين في + +289 +00:19:21,720 --> 00:19:27,120 +التقام الطب وفي كل يعني مجاري العامل الصحي، في قد + +290 +00:19:27,120 --> 00:19:33,310 +الواحدةالـ cepilococcus aureus، resistance to + +291 +00:19:33,310 --> 00:19:38,090 +methicillin من ال .. تم عزلها من أولوف المرضى and + +292 +00:19:38,090 --> 00:19:42,790 +medical staff in the dialysis unit in northwest .. + +293 +00:19:42,790 --> 00:19:49,140 +بالطفولة الغربية كانتمقاومة للميتسيلين وهذا مساعد + +294 +00:19:49,140 --> 00:19:52,940 +ال MSR .. MRSA colonization among patients and + +295 +00:19:52,940 --> 00:19:56,560 +stop in healthcare risk factor in addition of a .. + +296 +00:19:56,560 --> 00:19:59,240 +in addition to evaluating the relationship between + +297 +00:19:59,240 --> 00:20:04,000 +a pregnancy MRSA in the nose of employees in + +298 +00:20:04,000 --> 00:20:08,600 +healthcare and pollution caught same type ماعش + +299 +00:20:10,540 --> 00:20:14,380 +هذه الطبق كان قاوم .. يعني نمت على طبق موجود في + +300 +00:20:14,380 --> 00:20:17,620 +متسلم، + +301 +00:20:17,620 --> 00:20:22,160 +هذه بعض ال .. ال disease اللي بتصيب الأشخاص في ال + +302 +00:20:22,160 --> 00:20:23,020 +hemodialysis unit + +303 +00:20:27,020 --> 00:20:34,020 +وكمان هذا العظام الصديدي بيصيب home وهو بعض المرضى + +304 +00:20:34,020 --> 00:20:38,520 +يعني الأشخاص اللي بيكونوا معرضين أكتر من غير home + +305 +00:20:38,520 --> 00:20:41,360 +هم مرضى الدأ السكري، أنيميا المنجالية، مرضى الفشل + +306 +00:20:41,360 --> 00:20:44,220 +الكلوي، مرضى ال aids، مرضى السرطان، وادمين الكحلية + +307 +00:20:44,220 --> 00:20:46,960 +من المخدرات يعني، مرضى الفشل الكلوي هو من الأشخاص + +308 +00:20:46,960 --> 00:20:53,940 +المعرضين للمرض هذا، وهي بعض الصورو الأعراض بيكون + +309 +00:20:53,940 --> 00:20:57,780 +ألم و تورم و حمرار في مفصل واحد فقط تبدأ بشكل + +310 +00:20:57,780 --> 00:21:03,540 +مفاجئ و يكون مصاحب لها حمى ارتفاع درجة الحرارة بما + +311 +00:21:03,540 --> 00:21:06,080 +ان ال Staphylococcus aureus يعني هي مقاومة + +312 +00:21:06,080 --> 00:21:12,420 +للمضادات الحيوية فتنتقل من شخص لآخر و زيادة سيعة + +313 +00:21:12,420 --> 00:21:17,800 +في انتشار السلالات المقاومة للمضادات الحيوية تشكل + +314 +00:21:17,800 --> 00:21:20,260 +تهديد كبير على المرضى الموجودين + +315 +00:21:22,770 --> 00:21:25,430 +خوانات بعض الصور يعني أماكن موجودة و إن هم موجودين + +316 +00:21:25,430 --> 00:21:31,330 +في كل مكان بما إن ال ..istapilococcus aureus + +317 +00:21:31,330 --> 00:21:34,810 +مقاومة للمضادات الحيوية لازم يعني يجب على كل + +318 +00:21:34,810 --> 00:21:38,870 +العامل في الطقم الطبي و المرضى إن هم يعني يتبعوا + +319 +00:21:38,870 --> 00:21:42,190 +الخطوات التالية preventing istapilococcus aureus + +320 +00:21:42,190 --> 00:21:46,710 +is a transmission يعني إنه clean your hand take + +321 +00:21:46,710 --> 00:21:50,690 +care of infection keep your lines and cloth clean + +322 +00:21:51,690 --> 00:21:54,870 +don't share personal care items، يعني مانتشاركش أي + +323 +00:21:54,870 --> 00:22:02,110 +أغراض مع الأخرين، هذه خطوات أنه نغسل الصح ده يعني، + +324 +00:22:02,110 --> 00:22:06,550 +الأيد النظيفة تنقذ الأرواح، انه نعالج الاستفيل + +325 +00:22:06,550 --> 00:22:09,590 +الكوكاس أورياس بما أنها مقاومة للاستفيلمقاومة + +326 +00:22:09,590 --> 00:22:12,370 +للمضادات الحيوية، فلازم احنا نحط من ال toxin اللي + +327 +00:22:12,370 --> 00:22:20,410 +بتفرزه عن طريق انه ال oxasilane, sphalosforin يعني + +328 +00:22:20,410 --> 00:22:23,530 +ال sphalosforin و ال clindamycin، هاد ممكن نحط من + +329 +00:22:23,530 --> 00:22:31,490 +ال toxin اللي بتفرزه و فيه انه المضادات + +330 +00:22:31,490 --> 00:22:35,970 +اللي لازم انه على طاقم الطبي انهإنهم يعملوا يوم + +331 +00:22:35,970 --> 00:22:41,030 +غسيل لل .. للأيدي كما كان في جامعة كيتو اليابانية، + +332 +00:22:41,030 --> 00:22:44,270 +جامعة كيتو اليابانية حملة عالمية لغسل الأيدي عشان + +333 +00:22:44,270 --> 00:22:48,530 +انه تبنيها منظمة الصحة العالمية تحت شعار إنه نقض + +334 +00:22:48,530 --> 00:22:52,290 +مرضانا بغسل أيدينا و لازم إنه العاملين في الكادر + +335 +00:22:52,290 --> 00:22:57,190 +الطبي يهتموا بالنظافة و .. و ياخدوا دورات و توعيات + +336 +00:22:57,190 --> 00:22:57,730 +صحية + +337 +00:23:00,710 --> 00:23:03,810 +السلام عليكم ورحمة الله وبركاته معاكم طالبه رجاء + +338 +00:23:03,810 --> 00:23:07,330 +السيام رح نحكي ان شاء الله اليوم رح نتخصص في ال + +339 +00:23:07,330 --> 00:23:11,570 +microbiology رح نختصر موضوع ال food microbiology + +340 +00:23:11,570 --> 00:23:12,210 +ان شاء الله + +341 +00:23:15,050 --> 00:23:17,250 +أول إشي العنوان اللي عندنا لذكرها .. اول إشي + +342 +00:23:17,250 --> 00:23:20,610 +العنوان الأول اللي مر علينا ال food microbiology و + +343 +00:23:20,610 --> 00:23:23,990 +رح نحكي عن تعريفها و عندنا المصطرح التاني اللي + +344 +00:23:23,990 --> 00:23:26,790 +هنكون ان شاء الله بعد هاد ال presentation عندنا + +345 +00:23:26,790 --> 00:23:30,110 +خلفية عندنا ال foodborne infection و هنختصب ال + +346 +00:23:30,110 --> 00:23:34,330 +salmonellosis وهي عندنا الصورة هنا بتوضح صورة + +347 +00:23:34,330 --> 00:23:39,670 +البكتيريا ال salmonella و صورة إلها على الطبقرح + +348 +00:23:39,670 --> 00:23:42,690 +نبدأ بتعريف ال food microbiology ال food + +349 +00:23:42,690 --> 00:23:44,410 +microbiology اللي احنا ندرس اللي هي ال + +350 +00:23:44,410 --> 00:23:47,790 +microorganism اللي موجودة بال food ممكن ان هي تعمل + +351 +00:23:47,790 --> 00:23:52,110 +عندنا contamination لل food رح ال .. الشكل عام ال + +352 +00:23:52,110 --> 00:23:56,820 +microorganism اللي موجودة بال foodممكن تكون ضارة و + +353 +00:23:56,820 --> 00:23:59,720 +ممكن تكون مفيدة نبدأ من ناحية انها تكون مفيدة احنا + +354 +00:23:59,720 --> 00:24:03,520 +بنستخدم البكتيريا اللى بتحولنا ال liquid ال liquid + +355 +00:24:03,520 --> 00:24:06,660 +milk تحولنا ل yogurt و طبعا احنا عندنا لها ده في + +356 +00:24:06,660 --> 00:24:08,980 +هذا الوقت عندنا أطعمة كتيرة ممكن تكون بال + +357 +00:24:08,980 --> 00:24:11,960 +strawberry ممكن تكون بال banana ممكن تكون بال + +358 +00:24:11,960 --> 00:24:14,640 +chocolate و ايضا عندنا ال yeast من ال + +359 +00:24:14,640 --> 00:24:18,550 +microorganismاللي احنا بنستغل عملية ال + +360 +00:24:18,550 --> 00:24:23,470 +fermentation تبعتها و بتستعينها الخبز اللي هو ال + +361 +00:24:23,470 --> 00:24:29,230 +bread ايضا عندنا ال micro organism ممكن تكون ضارة + +362 +00:24:29,230 --> 00:24:32,270 +ممكن تعمل عندنا foodborne infection و ممكن تعمل + +363 +00:24:32,270 --> 00:24:35,930 +عندنا spoilage اللي هو فساد للاطعمة راح نتركها + +364 +00:24:35,930 --> 00:24:38,870 +لصور اللي هي من ناحية المفيدة لل micro organism + +365 +00:24:38,870 --> 00:24:41,350 +عندنا ال bread اللي هي من خلال ال yeast من خلال + +366 +00:24:41,350 --> 00:24:44,500 +عملية ال fermentationو ال .. و ال .. و ال .. و ال + +367 +00:24:44,500 --> 00:24:48,580 +liquid milk بتحول عندنا ل yogurt و طبعا عندنا منتج + +368 +00:24:48,580 --> 00:24:51,140 +جاهز بتعمل تسويقه في الأسواق + +369 +00:24:53,710 --> 00:24:56,090 +ال foodborne اللى هى ال disease اللى هى الأمراض + +370 +00:24:56,090 --> 00:24:58,770 +اللى بتكون عندنا راح نختص اليوم بالحديث ان شاء + +371 +00:24:58,770 --> 00:25:02,370 +الله عن ال salmonellosis وعندنا الطرق ال slide + +372 +00:25:02,370 --> 00:25:05,730 +اللى وراها راح تخرد عندنا طرق اللى احنا ممكن نحافظ + +373 +00:25:05,730 --> 00:25:09,910 +على الطعام بشكل آمن، نأمن على صحة الإنسان وهدى أهم + +374 +00:25:09,910 --> 00:25:12,750 +شغلها الصورة الأولى طبعا عندنا ال smoking اللى + +375 +00:25:12,750 --> 00:25:16,690 +عادة بتكون معروفة عندنا بتكون لل fish عندنا الهنف + +376 +00:25:16,690 --> 00:25:19,530 +ال low temperature لل vegetables لأنواع مختلفة من + +377 +00:25:19,530 --> 00:25:21,930 +ال vegetables و ال fruit و ال meat و ال ground + +378 +00:25:21,930 --> 00:25:25,240 +meat أيضاعنا الصورة اللى على الناحية التانية اللى + +379 +00:25:25,240 --> 00:25:28,000 +هى ال salting بين قوسين اللى هى المخللات اللى + +380 +00:25:28,000 --> 00:25:32,260 +مشهورة عنها مشهورة عنها ممكن نتطلع لشغلت تانية + +381 +00:25:32,260 --> 00:25:35,580 +اللى هى ال salting لل egg بين قوسين المخلل اللى + +382 +00:25:35,580 --> 00:25:39,640 +بعض الشعوب بتحب هذه الشغلات و بتكون عندها مشهورة + +383 +00:25:39,640 --> 00:25:42,480 +تتناولها أثناء الفطور أو أثناء العشاء + +384 +00:25:53,530 --> 00:25:57,030 +أيضا عندنا ال mountain british ممكن تكون ايضا لالي + +385 +00:25:57,030 --> 00:26:01,890 +فروز المواضح هنا والسوتل بما يعرف بالمربع و لدى + +386 +00:26:01,890 --> 00:26:05,190 +التشفيف عندنا كتير او نباتات ورقيه ممكن احنا + +387 +00:26:05,190 --> 00:26:09,090 +نجففها مثل الملوخيه والبعمل + +388 +00:26:10,930 --> 00:26:13,270 +ال group لل micro organism، مجموعة ال micro + +389 +00:26:13,270 --> 00:26:16,670 +organism اللي احنا فعلا ممكن نلاقيها بال food، اول + +390 +00:26:16,670 --> 00:26:19,790 +واحدة ال molds و ال yeast و البكتيريا و البرازيل و + +391 +00:26:19,790 --> 00:26:22,510 +ال internet virus، راح نتركك نتحدث عن البكتيريا + +392 +00:26:22,510 --> 00:26:27,340 +لإنها ال common في ال foodعنا مجموعة جدوى اللى + +393 +00:26:27,340 --> 00:26:29,900 +بوضح انها مجموعة من البكتيريا اللى ممكن نلاقيها + +394 +00:26:29,900 --> 00:26:33,380 +بال .. بال food البستيلا ال serious و بتعمل عينها + +395 +00:26:33,380 --> 00:26:36,520 +toxic و بنلاقيها موجودة فى الصوص و موجودة فى + +396 +00:26:36,520 --> 00:26:39,140 +البودن ال Campylobacter اللى هى بتعمل علينا + +397 +00:26:39,140 --> 00:26:42,540 +infection بتكون موجودة فى الملك و بال product نحكي + +398 +00:26:42,540 --> 00:26:46,220 +عن الملك خاصة بيكون مش مغلى و مش imposter فعشان + +399 +00:26:46,220 --> 00:26:50,740 +كده لازم نحزن ان احنا نغلى الحليب جيدا او نشتريه + +400 +00:26:50,740 --> 00:26:53,480 +من ايه يكون ال imposterراح ندرك الحديث عن + +401 +00:26:53,480 --> 00:26:56,460 +السالمونيلا Glostridium تعمل عنا توكسيك بتكون + +402 +00:26:56,460 --> 00:26:58,980 +موجودة في ال meat و ال beats عادة ال beats اللي + +403 +00:26:58,980 --> 00:27:03,380 +بتكون مطبوخة بطريقة غير جيدة لشلي شاكولاي و + +404 +00:27:03,380 --> 00:27:11,140 +species معينة منها 0157H7 و 0157MMبتكون موجودة + +405 +00:27:11,140 --> 00:27:14,600 +عندنا في ال ground meat اللي هي اللحمة المفرومة و + +406 +00:27:14,600 --> 00:27:17,340 +الجوسس لو كانت محضرة بطريقة غير جيدة أو إنه + +407 +00:27:17,340 --> 00:27:20,580 +الفواكه كانت مقصودة أيضا بطريقة غير جيدة ال + +408 +00:27:20,580 --> 00:27:23,480 +hysteria أيضا بتعمل عندنا infection و بتكون موجودة + +409 +00:27:23,480 --> 00:27:28,590 +في ال dilemma أو في ال hot dog النقانقأيضا عندنا + +410 +00:27:28,590 --> 00:27:31,190 +برضه الشيكية اللى بتعمل عندنا toxic و بتكون موجودة + +411 +00:27:31,190 --> 00:27:34,150 +في السلط و ال vegetables لو كانت .. لو كانت اللى + +412 +00:27:34,150 --> 00:27:38,670 +هو السلط غير .. المكونات السلط غير مغسولة بطريقة + +413 +00:27:38,670 --> 00:27:42,210 +جيدة راح نرجع للسالمون اللى هيتركز حديثنا عن + +414 +00:27:42,210 --> 00:27:44,950 +السالمون اللى بتعمل عندنا infection و بتكون موجودة + +415 +00:27:44,950 --> 00:27:48,470 +في نبات الزهرة لو كان مطبوخ بطريقة غير صحيحة أو مش + +416 +00:27:48,470 --> 00:27:52,430 +بتوفرله درجة الحرارة المناسبة تكون موجود في ال egg + +417 +00:27:52,430 --> 00:27:57,230 +و في ال raw meat و في ال .. ال chickenعينه ايضا + +418 +00:27:57,230 --> 00:28:01,230 +الهفادلسليد اللي اتعرض لل food borne disease اللي + +419 +00:28:01,230 --> 00:28:04,070 +هي اول اشي عن طريقه بتعمل عندنا infection او عن + +420 +00:28:04,070 --> 00:28:08,610 +طريق ال food borne intoxication في ال infection من + +421 +00:28:08,610 --> 00:28:10,950 +النوع في ال infection بتكون البكتيريا البنتوجين + +422 +00:28:10,950 --> 00:28:14,330 +احنا بنتناولها اما في ال intoxication قبل ما احنا + +423 +00:28:14,330 --> 00:28:20,390 +نتناول الطعام بتكون ال toxic موجود في الطعامطبعا + +424 +00:28:20,390 --> 00:28:23,590 +ممكن ال .. ال infection يكون ناتجة عن بكتيريا و + +425 +00:28:23,590 --> 00:28:27,570 +بتعمل عندنا cholera و salmonellosis أو بتكون ناتجة + +426 +00:28:27,570 --> 00:28:30,870 +عن اللي هو ال virus مثلا ال .. ال hepatitis + +427 +00:28:32,470 --> 00:28:35,410 +الثمونيلة نجم من نجام ال food microbiology بشكل + +428 +00:28:35,410 --> 00:28:38,490 +عربي هى غنية عن التعريف اللى هى عندنا gram + +429 +00:28:38,490 --> 00:28:44,110 +negative و gram negative و non sperm sperm form و + +430 +00:28:44,110 --> 00:28:47,170 +فالتتفان أوروبوكوموتاي بتعطينا positive ل .. + +431 +00:28:47,170 --> 00:28:50,430 +positive ل ال .. ل ال stress test و ايضا عندنا + +432 +00:28:50,430 --> 00:28:53,210 +اللى هى بتعمل gelicose fermentation و produce ال + +433 +00:28:53,210 --> 00:28:57,310 +H2S gas و هى بتدينا negative في الurease و + +434 +00:28:57,310 --> 00:29:01,490 +الindole testالسلمونيلة عادة بتلمع في درجات + +435 +00:29:01,490 --> 00:29:05,350 +الحرارة المتوسطة ولكنها حساسة لدرجة الحرارة + +436 +00:29:05,350 --> 00:29:09,010 +العالية لذلك يمكن القضاء عليها من خلال عملية ال + +437 +00:29:09,010 --> 00:29:14,360 +pasteurization و أيضا حساسة لل layer pHلا لو بي .. + +438 +00:29:14,360 --> 00:29:17,380 +لا لو بيقعدش قالنا شغلة مهمة جدا في ال + +439 +00:29:17,380 --> 00:29:20,640 +salmonellosis أنه ممكن تنمو في الطعام تنمي growth + +440 +00:29:20,640 --> 00:29:23,900 +إليه في الطعام بدون ما يتأثر على ال quality تبقى + +441 +00:29:23,900 --> 00:29:28,120 +الطعام وهدى شغلة مهمة جدا أو ممكن إحنا نعزلها أيضا + +442 +00:29:28,120 --> 00:29:32,980 +من ال water من ال water أو من ال soil من التربة أو + +443 +00:29:32,980 --> 00:29:38,500 +ال water اللى بتكون ليها contamination مقمع السوجو + +444 +00:29:38,500 --> 00:29:41,460 +عندها القدرة على ال invade و ال .. و ال damage للي + +445 +00:29:41,460 --> 00:29:44,900 +بتكون هي بتدخل عادة للجهاز الهضمي بتدخل لل .. + +446 +00:29:44,900 --> 00:29:48,600 +للأمعاء الدقيقة لل small intestine و بتعمل من خلال + +447 +00:29:48,600 --> 00:29:52,640 +انه .. انه production لل thermostable cytotoxic + +448 +00:29:52,640 --> 00:29:58,580 +factor بتوجد عادة باعداد كبيرة في ال food حتى تعمل + +449 +00:29:58,580 --> 00:29:59,340 +انه infection + +450 +00:30:02,820 --> 00:30:05,400 +هذه الصورة ده ان هي عالى تعريفه يعني راح تحكيلنا، + +451 +00:30:05,400 --> 00:30:08,460 +توضحنا بشكل عام، عين ال .. ال contamination food + +452 +00:30:08,460 --> 00:30:11,540 +دي لازم احنا اتنا ولاه، راح تدخل عادة ليه للجهاز + +453 +00:30:11,540 --> 00:30:14,100 +اللي هضمه، روح تختص في الصور في ال .. في ال small + +454 +00:30:14,100 --> 00:30:18,020 +intestine و تعمل lying و attachment فيها، و lying + +455 +00:30:18,020 --> 00:30:20,620 +و attachment، هي ده ال symptoms الأعراض عادة ان + +456 +00:30:20,620 --> 00:30:25,820 +بتدهرش مبكرا، بتدهر بعد من 12 ل 72 ساعة لانها + +457 +00:30:25,820 --> 00:30:29,930 +بتوقت حتى till oneو عادة الأعراض اللي بتشمالي هي + +458 +00:30:29,930 --> 00:30:33,670 +vomiting، دياريا، abdominal cramp ممكن يكون عندنا + +459 +00:30:33,670 --> 00:30:37,170 +قشريرة و هده حسب اللي هو عمره .. حسب الكمية اللي + +460 +00:30:37,170 --> 00:30:40,230 +كانت موجودة بالطعام و حسب عمر الإنسان اللي .. اللي + +461 +00:30:40,230 --> 00:30:44,630 +اتناول الطعام و حسب أيضا ال immune system عندهمال + +462 +00:30:44,630 --> 00:30:47,650 +illness range تختلف في المراجعة بس عادة إنها + +463 +00:30:47,650 --> 00:30:51,170 +اجتمعت إنه بتكون لمدة .. تستمر لمدة أسبوع و عادة + +464 +00:30:51,170 --> 00:30:54,450 +الإنسان من شفه منها إن الجسم بيحتويها بدون ما ياخد + +465 +00:30:54,450 --> 00:30:57,990 +أي treatment إلا إذا بحالات خاصة لو كان في عنده + +466 +00:30:57,990 --> 00:31:00,950 +severe diarrhea ممكن يدوه antibiotic .. antibiotic + +467 +00:31:00,950 --> 00:31:04,190 +اللي هو ال .. ال detection لل .. السلمون اللي في + +468 +00:31:04,190 --> 00:31:07,790 +ال food نعمل على pre enrichment للسمبل و بعدين في + +469 +00:31:07,790 --> 00:31:11,630 +نيوترانت أجار و بعدين عملنا سجلة selective الرشمةو + +470 +00:31:11,630 --> 00:31:15,210 +بعدين نعملها selective differential أجار و هاد + +471 +00:31:15,210 --> 00:31:19,710 +طبعا متأكدناش أنها موجودة بالظبط بال biochemical و + +472 +00:31:19,710 --> 00:31:23,350 +ال surgical test ليه نتأكد إن فعلا هي هاد بكتيريا + +473 +00:31:23,350 --> 00:31:26,530 +ال salmonella لأنه الأجار مابديناش نتيجة مؤكدة + +474 +00:31:26,530 --> 00:31:31,390 +ممكن نعتمد احنا عليهاهذه صور .. صور من مختبرات + +475 +00:31:31,390 --> 00:31:34,070 +الصحة كيف بياخدوا عينات، بياخدوا عينات ان القطر ما + +476 +00:31:34,070 --> 00:31:38,430 +بتنفحصها، الشعوب بتهتم ان تكون الصحة مناسبة + +477 +00:31:38,430 --> 00:31:42,170 +للإنسان و الصحة و سلامة الغذاء مناسبة، هي انا برضه + +478 +00:31:42,170 --> 00:31:45,490 +العاملين و انا في عينات اللي هي samples من ال food + +479 +00:31:45,490 --> 00:31:50,890 +بتنفحصهاLearn ways to prevent Salmonella infection + +480 +00:31:50,890 --> 00:31:54,610 +دي أهم شغلة و بدنا نأكد إنه الخطوات بسيطة وسهلة + +481 +00:31:54,610 --> 00:31:58,290 +جدا إحنا ممكن نتجنب فيها اللي هو ال Salmonella و + +482 +00:31:58,290 --> 00:32:01,750 +بنأكد على الحكمة اللي دايما كنا نكتبها اللي يدرها + +483 +00:32:01,750 --> 00:32:04,870 +الموقعية خير من قطار أيلاج + +484 +00:32:20,100 --> 00:32:24,240 +أنت تقنعني؟هي السلمونية لما تدخل جسم الإنسان و + +485 +00:32:24,240 --> 00:32:26,880 +يصير الواحد في عنده إصابة و فيها الإتناولة من خلال + +486 +00:32:26,880 --> 00:32:30,140 +الطعام و طبعا بتتم اللي هو مضاعفتها و نموها في + +487 +00:32:30,140 --> 00:32:33,600 +الأمعاق الدقيقة في جسم الإنسان و بتتسق فيها و + +488 +00:32:33,600 --> 00:32:38,820 +بتسببله ال infection هنا .. هنا بتوضح الصورة + +489 +00:32:38,820 --> 00:32:42,600 +لخطوات سيئة اللي ممكن إحنا نتبعها في المنزل هي مش + +490 +00:32:42,600 --> 00:32:45,940 +فاصلة عندنا ال raw .. ال raw food عن اللي هو اللي + +491 +00:32:45,940 --> 00:32:48,920 +ممكن إحنا نتناول القطر ما بتكون نية عن القطر .. + +492 +00:32:48,920 --> 00:32:50,500 +هنا برضه خطأ في التحضير + +493 +00:32:53,040 --> 00:32:56,800 +خطأ بسيطة و ممكن احنا نتجنبها، برضه هذه الصورة + +494 +00:32:56,800 --> 00:33:00,280 +بتوضح الأخطاء نتجنبها، انا ممكن يكون البحذر الطعام + +495 +00:33:00,280 --> 00:33:03,680 +يكون carrier أو الأدوات برضه بتكون مش نظيفة عشان + +496 +00:33:03,680 --> 00:33:05,320 +هيك لازم نحافظ على ال personal hygiene + +497 +00:33:09,810 --> 00:33:13,590 +لازم نعرف من وين بناكل و مين اللي بيحضرنا الأكل و + +498 +00:33:13,590 --> 00:33:17,110 +طبعا تغسلي الأيدي بالماء والصبور قبل تحضير الطعام + +499 +00:33:17,110 --> 00:33:20,390 +و بعد تحضير الطعام هنا لازم نفصل الطعام منه و + +500 +00:33:20,390 --> 00:33:24,010 +نجلتن و نطيم طريقة الحفظ اللي هو طريقة جيدة هنا + +501 +00:33:24,010 --> 00:33:26,430 +غسل البيت إيش مهم؟ هذه شغلة إحنا ما نسيها عندنا + +502 +00:33:26,430 --> 00:33:30,370 +تقريبا غسل + +503 +00:33:30,370 --> 00:33:33,890 +و أنا طبعا لازم غسل الأسطح .. غسل الأسطح اللي هو + +504 +00:33:33,890 --> 00:33:39,130 +بعد و قبل تحضير الطعاموشغلة برضه المهمة ان احنا + +505 +00:33:39,130 --> 00:33:42,470 +السكينة اللى بنستخدمها لتقطيع اللحمة او المكونات + +506 +00:33:42,470 --> 00:33:45,970 +النية مانستخدمهاش لتقطيع اللي هو الطعام المراد + +507 +00:33:45,970 --> 00:33:50,070 +تحضيره او تقديمه على الصفراء خلّيني أخرجك من ال + +508 +00:33:50,070 --> 00:33:50,390 +slide + +509 +00:33:53,440 --> 00:33:56,440 +هنا طبعا إنه لازم تكون الطعام محدد بدرجة حرارة + +510 +00:33:56,440 --> 00:33:59,480 +مناسبة وهنا اللي بشتغلوا في تحضير الطعام أوي إنه + +511 +00:33:59,480 --> 00:34:02,240 +بتم التغليف بطريقة جيدة وكلهم بتبقى personal + +512 +00:34:02,240 --> 00:34:06,160 +hygiene وهنا غسيل جيدا مش إنه بس نغسلها ونحطها تحت + +513 +00:34:06,160 --> 00:34:10,000 +الميه وغسيل الأيدي إش مهم جدا خطوة وسيطة وسهلة + +514 +00:34:10,000 --> 00:34:13,940 +ولكن لأ اللي يجب عدم التهاوان بها وهذا طبعا إش مهم + +515 +00:34:13,940 --> 00:34:17,380 +السورة إن إحنا السكينة اللي بنقطع فيها الرأو اللي + +516 +00:34:17,380 --> 00:34:20,870 +هوfood زي ال chicken مانعطلش في نفس السكينة و في + +517 +00:34:20,870 --> 00:34:23,710 +نفس المكان لازم نغسل يدوّد جيدا بالمي والسبوك و + +518 +00:34:23,710 --> 00:34:28,330 +بعدين نستخدمها في تبقي ال vegetables و بنأكد انه + +519 +00:34:28,330 --> 00:34:32,950 +ترها مقايا خيرا من قنطاري علاج و يعطيكوا العزيزي + +520 +00:34:32,950 --> 00:34:35,470 +السلام عليكم و مرحبا بكم جميعا و بركاته انا الطالب + +521 +00:34:35,470 --> 00:34:38,950 +علينا البلعوة بدأ احكيها عن موضوع كتير من المهم من + +522 +00:34:38,950 --> 00:34:43,580 +اليومهو موجود في كل مكان، أفواههم لا تخلوا منه + +523 +00:34:43,580 --> 00:34:48,700 +أبدا و بما أن زمبيبنا حكت قبل شوية عن الأكل و + +524 +00:34:48,700 --> 00:34:54,320 +الطريقة الصحية لتناول الغذاء و يجب أن نتطرق لما + +525 +00:34:54,320 --> 00:34:59,020 +بعد الأكل و هو تنظيف الأسماع و تجنب ما يسمى + +526 +00:34:59,020 --> 00:35:05,860 +بالدينة الكارثةهي تقريبا مشكلة متواجدة عند جميع + +527 +00:35:05,860 --> 00:35:11,920 +الناس، عند كل إنسان، فاحنا لازم نحكي عن مسببته + +528 +00:35:11,920 --> 00:35:18,420 +وقليل حدوثه، فأنا اخترت هذا الموضوع لهذا الشرطة، + +529 +00:35:18,420 --> 00:35:21,940 +فمسببته هي كائنات دقيقة + +530 +00:35:25,120 --> 00:35:32,600 +تسمى ب Stryptococcus Mutans و Lactobacillus + +531 +00:35:32,600 --> 00:35:39,440 +Acidobacillus رح تستغرب شوية بس ساعدنا بنشوفه + +532 +00:35:39,440 --> 00:35:46,300 +بداية بدنا نحكي عن صفات كل كائن لحد فال + +533 +00:35:46,300 --> 00:35:49,780 +Stryptococcus Mutans هو غالي عن التعريف احنا + +534 +00:35:49,780 --> 00:35:55,600 +اتعرفنا عليه بشكلسريع قبل هيك يعني، فمن صفات الـ + +535 +00:35:55,600 --> 00:35:58,640 +streptococcus mutant هو facultative anaerobic، + +536 +00:35:58,640 --> 00:36:04,500 +ground positive، هو موجود بشكل رئيسي، بالفهم هو + +537 +00:36:04,500 --> 00:36:08,180 +المسبب الرئيسي كمان لتصوص الأسنان، الدين تلكاس أو + +538 +00:36:08,180 --> 00:36:08,960 +الدين تلتكان + +539 +00:36:11,640 --> 00:36:14,320 +الlactobacillus هو اللي ممكن تستغربوا شوية ان انا + +540 +00:36:14,320 --> 00:36:18,340 +هحكيلكوا انه موجود بالفم برضه، احنا عرفنا قبل هيك + +541 +00:36:18,340 --> 00:36:24,980 +انه موجود في البطانيا الجوفا لل .. لل .. للحلق، ده + +542 +00:36:24,980 --> 00:36:27,960 +انا كانت زميلة قبل هيك، وكان كمان موجود في ال + +543 +00:36:27,960 --> 00:36:28,900 +gastrointestinal + +544 +00:36:35,250 --> 00:36:38,790 +فبنحكي عن صفاته بداية ان ال luck to pass الناس هو + +545 +00:36:38,790 --> 00:36:45,370 +grab positive، facultative على أوروبا على أوروباهو + +546 +00:36:45,370 --> 00:36:48,950 +موجود حاكينا إنه بشكل أساسي موجود في ال + +547 +00:36:48,950 --> 00:36:53,410 +gastrointestinal tract هو يتواجد ك normal flora + +548 +00:36:53,410 --> 00:36:59,170 +لكن في حال تواجده بالفم هو يصبح مضر بطريقة غير + +549 +00:36:59,170 --> 00:37:05,310 +مباشرة هو اسمه lactobacillus يعني بيحول ال lactose + +550 +00:37:05,310 --> 00:37:10,840 +إلى lactic acid فبيخفض ال pHلما يخفض ال pH هيسبب + +551 +00:37:10,840 --> 00:37:16,900 +يعني نمو الكائنات بتعيش عن درجة pH معينة فهيسبب ال + +552 +00:37:16,900 --> 00:37:25,860 +dental caress يعني بطريقة غير مباشرة راح + +553 +00:37:25,860 --> 00:37:29,640 +نحكي عن ال principle بشكل سريع يعني مش هطول عليكوا + +554 +00:37:30,250 --> 00:37:34,250 +رح نحكي على ال principle كيف رح يصير الهنفة كارثة، + +555 +00:37:34,250 --> 00:37:39,090 +فبعد تناول وشبة من الغذاء مليئة بال carbohydrate + +556 +00:37:39,090 --> 00:37:44,470 +والسكريات نتيجة + +557 +00:37:44,470 --> 00:37:50,410 +عدم التنظيف المستمر رح يتركم هذا .. رح يتركم على + +558 +00:37:50,410 --> 00:37:54,690 +الأسنان يعني بقايا من هذا الطعام، مع مرور الوقت رح + +559 +00:37:54,690 --> 00:38:01,260 +يتكون ما يسمى بالهنفة ال blackراح يتعمل ما يسمى + +560 +00:38:01,260 --> 00:38:07,520 +بالـ Dental Brachium تكون على شكل biofilm تتكوّن + +561 +00:38:07,520 --> 00:38:14,160 +من أكثر من 600 كائن بتتنافس على الحياة في وسط + +562 +00:38:14,160 --> 00:38:20,580 +مناسب و لائم تتوفر فيه pH مناسبة، Oxygen مناسب و + +563 +00:38:20,580 --> 00:38:22,180 +غذاء مناسب أيضا + +564 +00:38:29,270 --> 00:38:33,510 +تستطيع هذه البكتيريا المنافسة على المعيشة بحيث + +565 +00:38:33,510 --> 00:38:38,950 +تصبح لها .. تصبح بسيط لدرجة تكون قادرة على الهيمنة + +566 +00:38:38,950 --> 00:38:43,710 +و المنافسة على البقاء و الاستمرار فتسب .. فتقوم + +567 +00:38:43,710 --> 00:38:49,670 +هذه البكتيريا بعد الهيمنة تقوم بخضم الساكراز إلى + +568 +00:38:49,670 --> 00:38:55,550 +lactic acid باستخدام انزين لجلوكوز، glucan، ساكراز + +569 +00:38:55,820 --> 00:39:02,160 +فيسبب بما يسمى بالـ mineralized tooth enamel تسبب + +570 +00:39:02,160 --> 00:39:07,760 +بتحلل في طبقات السن فتضع في الطبقات مقاومة ما يسمى + +571 +00:39:07,760 --> 00:39:13,030 +بالاستيكيل مما أدى إلى tooth decayأنا حكيتلكوا إنه + +572 +00:39:13,030 --> 00:39:16,830 +موضوع صغير و مش راح أطول عليكم كتير فيه، هذه من + +573 +00:39:16,830 --> 00:39:21,470 +ضمن ال principle برضه، بس قبل ما أنهي، أنا حكيت ال + +574 +00:39:21,470 --> 00:39:25,870 +principle، حكيت عن .. عن خصائص الفكتيريا، قبل ما + +575 +00:39:25,870 --> 00:39:30,510 +أنهي، لفت نظري موضوع كتير صغير يعني بس مهم، كانوا + +576 +00:39:30,510 --> 00:39:35,810 +يحذرونا منه، و الرسول كمان أهانى عنه إن احنا ننفق + +577 +00:39:35,810 --> 00:39:41,800 +.. هو يعني خارج الإطارداخله يعني، نخانة عنده + +578 +00:39:41,800 --> 00:39:45,820 +الرسول يمون يعني، حكينا إنه لا تنفخوا بالإناء أو + +579 +00:39:45,820 --> 00:39:49,020 +.. لا تنفخوا .. لا تنفخوا بالإناء، احنا الناس + +580 +00:39:49,020 --> 00:39:52,020 +بيحكوا ما تنفخوش على الطعام الساخن، ما سألت نفسي + +581 +00:39:52,020 --> 00:39:56,880 +كلش يعني؟أنا كتير يعني هذا .. أه احنا بعد هذا ال + +582 +00:39:56,880 --> 00:39:59,840 +course أنا قدرت أني أفسر هذه الشغلة و أفهم ليش + +583 +00:39:59,840 --> 00:40:02,180 +كانوا يحكوا أنها ماتنفخوا يعني على الأكل الساخن + +584 +00:40:02,180 --> 00:40:07,020 +فهمت أنه بعد ما ننفخ و حسب مقارنة يعني بعد ما ننفخ + +585 +00:40:07,020 --> 00:40:11,680 +على الطعام الساخن راح تخرج ال normal flora من الفم + +586 +00:40:12,040 --> 00:40:16,960 +رح تتفاعد مع الأكل الساخن بطريقة معينة يعني، بعد + +587 +00:40:16,960 --> 00:40:20,180 +ما نرجع ناطلوه، احنا حكينا بعد انتقال ال normal + +588 +00:40:20,180 --> 00:40:24,760 +flora لمكان أخر غير مكان هتطبيه تصبح ضارة، بالتالي + +589 +00:40:24,760 --> 00:40:28,700 +هي هتصبح ضارة بطريقة فظيعة، هي هتسبب مرض السكري + +590 +00:40:28,700 --> 00:40:32,120 +وكمان قرحة في المعيدة. شكرا، ان شاء الله كنت + +591 +00:40:32,120 --> 00:40:37,090 +مستفيدةفي الغتام بالصحيح أولا اتقدم بالعزيزي الشكر + +592 +00:40:37,090 --> 00:40:41,290 +للطالبات اللي شاركوا في هذه ال .. ال .. الأروض، + +593 +00:40:41,290 --> 00:40:47,590 +البعض منهم كان يعني طبعا هو متوتر، البعض منهم كان + +594 +00:40:47,590 --> 00:40:53,070 +بتكلم اللغة الإنجليزية و هو لاسمعها يعنيفلكن بشكل + +595 +00:40:53,070 --> 00:40:57,750 +عام المواضيع متوفرة عليها الصحيح وجيدة ويعني أتمنى + +596 +00:40:57,750 --> 00:41:00,710 +من إنه بالفعل يعني يستفيد منها لإن هذه عبارة عن + +597 +00:41:00,710 --> 00:41:04,970 +أنشطة لها منهجية تقوي الشخص، في كانت اللي هو + +598 +00:41:04,970 --> 00:41:10,600 +إنجليزية كمان كويتا ومترددة للصحيحففيه أننا .. فيه + +599 +00:41:10,600 --> 00:41:13,280 +أننا أقصد من ال .. من .. من المواضيع اللي خارج + +600 +00:41:13,280 --> 00:41:17,700 +إطار المنهج هي مواضيع قد نستفيد منها أكتر من منهج + +601 +00:41:17,700 --> 00:41:21,760 +أدان فاستمروا على ذلك ال .. ال .. النهج في اختيار + +602 +00:41:21,760 --> 00:41:27,180 +مواضيع مميزة، البعض منكم يستطيع أن يحول موضوع إلى + +603 +00:41:27,180 --> 00:41:31,500 +مقال يمكن له أن ينشر، يعني مثلا ال botcherism في + +604 +00:41:31,500 --> 00:41:32,840 +الأطفال + +605 +00:41:34,700 --> 00:41:42,080 +الأطفال لماذا لا ينصح ليعطاء العسل للرقبة؟ + +606 +00:41:42,080 --> 00:41:46,720 +هذا موضوع من المواضيع اللي بتساهم في موضوع ال + +607 +00:41:46,720 --> 00:41:50,290 +infant botulismالـ Caffeine-degrading bacteria + +608 +00:41:50,290 --> 00:41:54,090 +موضوع attractive و could be برضه كمان transformed + +609 +00:41:54,090 --> 00:41:59,370 +فنقصد ان الموضوع كله رغم اني درست بعض العناوين لكن + +610 +00:41:59,370 --> 00:42:03,670 +في الآخر انا شخصيا استمتعت و استفدت و لجعت في + +611 +00:42:03,670 --> 00:42:06,050 +الخلف فاشكركم والسلام عليكم و رحمة الله و بركاته + diff --git a/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/xg8iBc9OMFs_raw.json b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/xg8iBc9OMFs_raw.json new file mode 100644 index 0000000000000000000000000000000000000000..eb2e6472c2fa450d627616fbc266fc7e8cb3799d --- /dev/null +++ b/PL9fwy3NUQKwbfQ5wapadJXYQLPZzCQDJa/xg8iBc9OMFs_raw.json @@ -0,0 +1 @@ +{"segments": [{"id": 1, "seek": 4027, "start": 21.19, "end": 40.27, "text": "بسم الله الرحمن الرحيم الحمد لله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله وبرحمة الله", "tokens": [3555, 38251, 21984, 34892, 5016, 27842, 34892, 5016, 32640, 21542, 2304, 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We found biofilm on teeth as a blaze or submerged on rocks in a stream. 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"text": "تسمى ب Stryptococcus Mutans و Lactobacillus Acidobacillus رح تستغرب شوية بس ساعدنا بنشوفه بداية بدنا نحكي عن صفات كل كائن لحد فال Stryptococcus Mutans هو غالي عن التعريف احنا اتعرفنا عليه بشكل", "tokens": [2655, 38251, 7578, 4724, 745, 627, 662, 905, 905, 1149, 18517, 599, 4032, 441, 578, 996, 326, 373, 301, 5097, 327, 996, 326, 373, 301, 12602, 5016, 6055, 14851, 17082, 25513, 13412, 2407, 10632, 4724, 3794, 8608, 995, 22488, 8315, 4724, 1863, 8592, 38688, 3224, 4724, 28259, 10632, 47525, 8315, 8717, 5016, 4117, 1829, 18871, 20328, 5172, 9307, 28242, 9122, 16373, 1863, 5296, 24401, 6156, 6027, 745, 627, 662, 905, 905, 1149, 18517, 599, 31439, 32771, 6027, 1829, 18871, 16712, 3615, 16572, 5172, 1975, 5016, 8315, 1975, 2655, 3615, 28480, 8315, 47356, 4724, 8592, 28820], "avg_logprob": -0.23795573273673654, "compression_ratio": 1.5621621621621622, "no_speech_prob": 2.384185791015625e-07, "words": [{"start": 2125.12, "end": 2125.86, "word": "تسمى", "probability": 0.87060546875}, 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