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""" |
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This dataset contains example data for running through the multiplexed imaging data pipeline in |
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Ark Analysis: https://github.com/angelolab/ark-analysis. |
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Dataset Fov renaming: |
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TMA2_R8C3 -> fov0 |
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TMA6_R4C5 -> fov1 |
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TMA7_R5C4 -> fov2 |
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TMA10_R7C3 -> fov3 |
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TMA11_R9C6 -> fov4 |
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TMA13_R8C5 -> fov5 |
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TMA17_R9C2 -> fov6 |
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TMA18_R9C2 -> fov7 |
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TMA21_R2C5 -> fov8 |
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TMA21_R12C6 -> fov9 |
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TMA24_R9C1 -> fov10 |
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""" |
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import datasets |
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import pathlib |
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_CITATION = """\ |
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@InProceedings{huggingface:dataset, |
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title = {Ark Analysis Example Dataset}, |
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author={Angelo Lab}, |
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year={2022} |
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} |
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""" |
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_DESCRIPTION = """\ |
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This dataset contains 11 Field of Views (FOVs), each with 22 channels. |
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""" |
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_HOMEPAGE = "https://github.com/angelolab/ark-analysis" |
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_LICENSE = "https://github.com/angelolab/ark-analysis/blob/main/LICENSE" |
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_URL_DATA = { |
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"image_data": "./data/image_data.zip", |
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"cell_table": "./data/segmentation/cell_table.zip", |
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"deepcell_output": "./data/segmentation/deepcell_output.zip", |
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"example_pixel_output_dir": "./data/pixie/example_pixel_output_dir.zip", |
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"example_cell_output_dir": "./data/pixie/example_cell_output_dir.zip", |
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"spatial_lda": "./data/spatial_analysis/spatial_lda.zip", |
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"post_clustering": "./data/post_clustering.zip" |
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} |
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_URL_DATASET_CONFIGS = { |
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"segment_image_data": {"image_data": _URL_DATA["image_data"]}, |
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"cluster_pixels": { |
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"image_data": _URL_DATA["image_data"], |
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"cell_table": _URL_DATA["cell_table"], |
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"deepcell_output": _URL_DATA["deepcell_output"], |
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}, |
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"cluster_cells": { |
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"image_data": _URL_DATA["image_data"], |
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"cell_table": _URL_DATA["cell_table"], |
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"deepcell_output": _URL_DATA["deepcell_output"], |
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"example_pixel_output_dir": _URL_DATA["example_pixel_output_dir"], |
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}, |
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"post_clustering": { |
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"image_data": _URL_DATA["image_data"], |
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"cell_table": _URL_DATA["cell_table"], |
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"deepcell_output": _URL_DATA["deepcell_output"], |
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"example_cell_output_dir": _URL_DATA["example_cell_output_dir"], |
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}, |
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"fiber_segmentation": { |
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"image_data": _URL_DATA["image_data"], |
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}, |
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"LDA_preprocessing": { |
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"image_data": _URL_DATA["image_data"], |
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"cell_table": _URL_DATA["cell_table"], |
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}, |
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"LDA_training_inference": { |
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"image_data": _URL_DATA["image_data"], |
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"cell_table": _URL_DATA["cell_table"], |
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"spatial_lda": _URL_DATA["spatial_lda"], |
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}, |
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"neighborhood_analysis": { |
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"image_data": _URL_DATA["image_data"], |
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"cell_table": _URL_DATA["cell_table"], |
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"deepcell_output": _URL_DATA["deepcell_output"], |
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}, |
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"pairwise_spatial_enrichment": { |
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"image_data": _URL_DATA["image_data"], |
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"cell_table": _URL_DATA["cell_table"], |
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"deepcell_output": _URL_DATA["deepcell_output"], |
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"post_clustering": _URL_DATA["post_clustering"], |
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} |
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} |
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class ArkExample(datasets.GeneratorBasedBuilder): |
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"""The Dataset consists of 11 FOVs""" |
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VERSION = datasets.Version("0.0.4") |
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BUILDER_CONFIGS = [ |
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datasets.BuilderConfig( |
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name="segment_image_data", |
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version=VERSION, |
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description="This configuration contains data used by notebook 1 - Segment Image Data.", |
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), |
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datasets.BuilderConfig( |
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name="cluster_pixels", |
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version=VERSION, |
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description="This configuration contains data used by notebook 2 - Pixel Clustering (Pixie Pipeline #1).", |
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), |
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datasets.BuilderConfig( |
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name="cluster_cells", |
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version=VERSION, |
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description="This configuration contains data used by notebook 3 - Cell Clustering (Pixie Pipeline #2).", |
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), |
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datasets.BuilderConfig( |
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name="post_clustering", |
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version=VERSION, |
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description="This configuration contains data used by notebook 4 - Post Clustering.", |
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), |
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datasets.BuilderConfig( |
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name="fiber_segmentation", |
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version=VERSION, |
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description="This configuration contains data used by the Fiber Segmentation Notebook.", |
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), |
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datasets.BuilderConfig( |
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name="LDA_preprocessing", |
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version=VERSION, |
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description="This configuration contains data used by the Spatial LDA - Preprocessing Notebook." |
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), |
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datasets.BuilderConfig( |
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name="LDA_training_inference", |
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version=VERSION, |
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description="This configuration contains data used by the Spatial LDA - Training and Inference Notebook." |
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), |
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datasets.BuilderConfig( |
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name="neighborhood_analysis", |
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version=VERSION, |
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description="This configuration contains data used by the Neighborhood Analysis Notebook." |
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), |
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datasets.BuilderConfig( |
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name="pairwise_spatial_enrichment", |
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version=VERSION, |
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description="This configuration contains data used by the Pairwise Spatial Enrichment Notebook." |
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) |
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] |
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def _info(self): |
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if self.config.name in [ |
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"segment_image_data", |
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"cluster_pixels", |
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"cluster_cells", |
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"post_clustering", |
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"fiber_segmentation", |
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"LDA_preprocessing", |
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"LDA_training_inference", |
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"neighborhood_analysis", |
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"pairwise_spatial_enrichment", |
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]: |
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features = datasets.Features( |
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{f: datasets.Value("string") for f in _URL_DATASET_CONFIGS[self.config.name].keys()} |
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) |
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else: |
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ValueError(f"Dataset name is incorrect, options include {list(_URL_DATASET_CONFIGS.keys())}") |
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return datasets.DatasetInfo( |
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description=_DESCRIPTION, |
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features=features, |
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homepage=_HOMEPAGE, |
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license=_LICENSE, |
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citation=_CITATION, |
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) |
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def _split_generators(self, dl_manager): |
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urls = _URL_DATASET_CONFIGS[self.config.name] |
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data_dirs = {} |
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for data_name, url in urls.items(): |
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dl_path = pathlib.Path(dl_manager.download_and_extract(url)) |
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data_dirs[data_name] = dl_path |
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return [ |
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datasets.SplitGenerator( |
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name=self.config.name, |
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gen_kwargs={"dataset_paths": data_dirs}, |
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), |
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] |
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def _generate_examples(self, dataset_paths): |
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yield self.config.name, dataset_paths |
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