sentence: They were then air-dried , cross-linked by Spectrolinker ™ XL-1000 ( 120 mJ / cm ) , and detected with anti-5hmC ( Active motif , Cat # 39769 , 1:5,000 ) or anti-5mC ( Eurogentec , Cat # BI-MECY-0100 , 1:2,500 ) antibodies .