sentence: The 25 μ l ddPCR reactions contained Supermix for Probes ( BioRad Laboratories ) , forward primer ( AdV : 700 nmol l , GGA CGC CTC GGA GTA CCT GAG ; PyV : 600 nmol l , AGT CTT TAG GGT CTT CTA CCT TT ) , reverse primer ( AdV : 700 nmol l , ACG GTG GGG TTT CTG AAC TTG TT ; PyV : 600 nmol l , GGT GCC AAC CTA TGG AAC AG ) , 250 nmol l probe ( AdV : 6FAM-CTGGTGCAGTTCGCCCGTGCCA-BHQ ; PyV : 6FAM-TCATCACTGGCAAACAT-MGB ) and 5 μ l of sample .