sentence: FigTree v. 1.2.3 software was used to display the generated tree.To elucidate structural differences between the three clusters of AIG1 family proteins in strain HM-1 , entire sequences of the 28 AIG1 family proteins were aligned using MAFFT ( v7.351b ) [ 82 ] , and visualized using MSAViewer [ 83 ] or NCBI Multiple Sequence Alignment Viewer 1.6.0.3D-models of AIG1 domains in EHI_176590 , EHI_129470 , and EHI_022500 were constructed using FORTE [ 31 ] , with Modeller ( 9v8 ) based on alignments between the query and GIMAPs , AIG1 domain containing protein from mammals ( PDB ID codes 2XTP ( GIMAP2 ) , and 3ZJC ( GIMAP7 ) .