sentence: Analysis of the LCB profiles by HPLC following methanolic acid hydrolysis of protonemata and gametophores and extraction of liberated LCBs demonstrated that all sbh mutant lines contained essentially undetectable amounts of t18 :0 , and both sbh protonemata and gametophores were highly enriched in d18 :0 ( Figures 2 and 3 ) .