sentence: Six 10 cm plates of HCT116 cells were used for each condition and samples of the PSE fractions [ 200 μ l of SN1 , 200 μ l of SN2 , and 100 μ l of SN3 ( taken to 200 μ l with SN3 buffer ) ] were ultracentrifuged at 155,000 × g in a TLA-100 rotor for 120 min at 4 ° C. Pellets were resuspended in the same original buffer and volume .