sentence: A fully phosphorothiolated 18 bp LNA / DNA mixmer ( Exiqon ) ( sequence provided in Supplementary Table 5 ) containing 7 LNA bases predicted to block the binding of miR-151-3p to the target E2f6 3 ′ UTR site without inducing RNAse H cleavage was cotransfected in MEF cells at 50 nM using Trans IT-TKO ( Mirus Bio ) .