sentence: In order to clarify the phylogenetic relationships of the newly-sequenced Nakaseomyces and 17 other Saccharomycotina , we used two alternative phylogenomics approaches , namely i ) a Maximum Likelihood ( ML ) analysis of a concatenated alignment of 603 protein families that have one-to-one orthologs in all the species considered , and ii ) a super-tree approach based on the analysis of 4,965 individual gene trees , which finds the species topology that is most parsimonious in terms of implied duplication events in all the individual gene trees [ 20 ] .