sentence: RNA associated with fibrillarin in each experimental condition from the four independent experiments was analyzed by RT-qPCR.For RT-qPCR analysis in Fig. 7b – g , transfections with TFIP11 siRNA ( siTFIP11 # 1 or siTFIP11 # 2 ) or control siRNA ( siCtr ) or mock transfections were independently performed three times .