sentence: Frameshift correction tools such as FrameBot ( 31 ) , HMM-Frame ( 32 ) , and FragGeneScan ( 33 ) are available to correct these sequencing artifacts and also predict protein-coding regions in short reads.The low performance of the profile-based approach ( HMM ) versus that of BLASTx ( Table 1 ) is presumably attributable to the lower sensitivity of the former with ( i ) short sequences , ( ii ) sequencing errors creating frameshifts , and ( iii ) a reduced fraction of highly conserved amino acid residues specific to the protein of interest , as suggested previously ( 32 , 34 ) .