sentence: The lines of evidence that show TpbA represses c-di-GMP production in P. aeruginosa that we found are ( i ) inactivating tpbA increases c-di-GMP ( Fig. S3 ) ; ( ii ) inactivating tpbA increases biofilm formation ( Fig. 1 ) , EPS production ( Fig. 3B ) , and pellicle formation , and c-di-GMP stimulates biofilm formation [ 4 ] , EPS production [ 6 ] , and pellicle formation [ 4 ] ; ( iii ) inactivating tpbA increases expression of the pel locus ( seen via the whole-transcriptome analysis and RT-PCR ) , and c-di-GMP activates expression of pelA [ 6 ] ; ( iv ) inactivating tpbA increases aggregation ( Fig. 4 ) and expression of adhesins ( PA4625 ) , and c-di-GMP stimulates adhesion [ 40 ] ; ( v ) inactivating tpbA decreases motility ( abolishing swarming and decreasing swimming in the tpbA mutant , Fig. 2AB ) , and c-di-GMP decreases swarming [ 40 ] ; ( vi ) inactivating tpbB ( encodes a GGDEF-motif protein that produces c-di-GMP [ 4 ] ) suppresses the phenotypes observed in the tpbA mutant , and ( vii ) expression of tpbA and tpbB in trans complements aggregation / biofilm formation and aggregation , respectively .