sentence: Cells were grown in rich medium ( YPAD or YPAGal ) to log phase and MMS ( at indicated concentrations ) was added to the half of each culture , which were further incubated until the saturation point was reached ( 24 hours for wild-type , ubp10 Δ and ubp10 Δ rev3 Δ strains in Figure S14 to 48 hours for wild-type , GAL1 ,10 : UBP10 , rev3 Δ and GAL1 ,10 : UBP10 rev3 Δ strains in Figure S6 ) .