sentence: The following fly strains were used : ( 1 ) w , ( 2 ) VASA-GFP / CyO ; Pr Dr / TM3 , Sb , ( 3 ) VASA-GFP / CyO ; smg / TM3 , Sb , ( 4 ) Df ( 3L ) Scf-R6 / TM3 , Sb ( Bloomington Stock # 4500 ) , ( 5 ) smg / TM3 ( Chen et al. 2014 ) , and ( 6 ) w ; P [ FLAG.HA.AGO2 ] 2 ( Bloomington Stock # 33242 ) .