sentence: Both fusion proteins can be cleaved with HRV 3C protease to release NMT.The recombinant plasmid encoding a His-tagged LdNMT was introduced into E. coli BL21 ( DE3 ) pRareS , and expression was achieved by growth in an autoinduction medium41 with overnight growth at 18 ° C. Cell lysates were prepared by sonication in a buffer containing protease inhibitors , bovine pancreatic DNaseI , and a nonionic surfactant , Triton X-100 [ 0.1 % ( v / v ) ] .