sentence: The three independent RNA samples from the two experimental conditions were then analyzed by RT-qPCR using specific primers.Transfections with TFIP11 siRNA ( siTFIP11 # 1 or siTFIP11 # 2 ) , Coilin siRNA ( siCoilin ) , control siRNA ( siCtr ) or mock transfections ( no siRNA ) followed by western blots of relevant proteins were independently performed 3 times .