sentence: The full length ORF of CaHSL1 , CaWRKY40 or ( with or without the termination codon ) were cloned to the entry vector pDONR207 by BP reaction , then to various destination vectors including pEarleyGate201 , pEarleyGate103 ( containing a GFP protein tag for subcellular localization ) or pEarleyGate202 [ containing a FLAG protein tag for chromatin immunoprecipitation ( ChIP ) analysis ] by LR reaction using a gateway cloning technique ( Invitrogen , Carlsbad , CA , United States ) .