sentence: Using Western blot analyses with antibodies to well-known RBFs of the 40S and 60S preribosome maturation ladder ( for a scheme , see Supplementary Fig. 1a ; for subcellular localization and other known characteristics of RBFs , see Supplementary Fig. 1b ) , we demonstrated that this method can efficiently extract both 40S ( TBL3 , FBL , ENP1 , RRP12 , TSR1 , LTV1 , RIO2 , NOB1 ) and 60S ( PES1 ) RBFs .