sentence: However , the strain with Dke1 Y21W ( Q3082 ) only produced 28.8 ± 1.0 mg / L acetylacetone , and the production reduced dramatically to 7.0 ± 0.4 mg / L when Dke1 S17D was used in strain Q3081.Meanwhile , 3D structure analysis of the wild-type Dke1 was performed based on its crystal structure ( PDB ID : 3BAL ) .