sentence: Three + / Δ NMT : : HYG clones ( H1 , H4 and H16 ) were independently transfected with pTEX NEO ( neomycin ) NMT7 to generate clones overexpressing NMT from the pTEX plasmid ( H1 , H4 , H16 + / Δ NMT : : HYG [ pTEX NEO NMT ] ) .