sentence: The target genes ( cscB , flv3 , sps ) were ordered as synthetic DNA fragments ( GenScript , US ) , and expressed in Synechocystis from an autonomously replicating pDF-lac2 plasmid [ 29 ] under the isopropyl β - D-1-thiogalactopyranoside ( IPTG ) - inducible promoter P and RBS from Synechocystis cpcB .