sentence: In vitro translation reactions were assembled in a total volume of 12.5 μ l that includes 100 ng of luciferase mRNA ( 0.19 pmol ) , 50 ng of recombinant SBP2 ( amino acids 399 – 846 ) , 8.25 μ l of nuclease-treated rabbit reticulocyte lysate ( RRL ) ( Promega ) , complete amino acid mixture and RNA guard as described in ( 37 ) .