sentence: HeLa cells were transfected with plasmids carrying wild-type ( wt ) or mutant ACBD3 ( FQ , mut1 , mut2 , mut3 ) , wt or mutant PI4KB ( I A , D A ) , Golgi-targeting EGFP ( pEGFP-GalT ) , or kinase-dead PI4KB ( PI4KB-KD ) as a negative control .