sentence: Fold changes and statistical calculations stated in the text were calculated with REST2009 as this program consistently demonstrated higher stringency for significance than the 2 method with t-test.We observed significant down-regulation of insulin-like growth factor II ( IGF2 , fold change = 18.52 , p = 0.003 , Figure 2A ) in our poor-responder cohort ( DFI < 100 ) .