sentence: After isolation , RNAsecure ( ThermoFisher AM7006 ) was added to the RNA and samples heated for 10 min at 60 ° C. Samples cooled down on ice and then placed in − 80 ° C.The quality and quantity of total RNA samples were assessed and analyzed using the Total RNA 6000 Nano Chip for the Agilent 2100 Bioanalyser .