sentence: Genomic DNA was isolated from the clones and used as a template for PCR with primers JG402 / JG407 for HP0381 , JG378 / JG383 for HP1369 , JG384 / 389 for HP1471 , JG390 / JG395 for HP1522 , and JG396 / JG401 for HP0464 to screen for deletion of the target gene.To construct mutants in which the homopolymeric tract had been replaced a strategy using replacement of the wild-type allele with rpsL-cat with a PCR amplicon encoding the mutant allele was pursued .