sentence: Interestingly , lariatins also have some of these conserved residues such as the Phe residue at position − 6 and the Gly residue at position − 8. Other Gram-positive bacteria-producing lasso peptides sviceucin , siamycin I and aborycin similarly contain these residues of Thr-2 , Tyr / Phe-6 and Gly-84525 , indicating that these residues in the leader peptide sequences have been conserved cross phylum.Furthermore , the production analysis of the lariatin variants demonstrated that Gly1 , Arg7 Glu8 , and Trp9 seem to be needed for maturation of the precursor peptides by the biosynthetic machinery ( Figs 3 and S9 ) .