sentence: The membrane was blocked with 5 % non fat dry milk in TBST { Tris-buffered saline ( 10 mM Tris , 100 mM NaCl ) , containing 0.1 % Tween-20 } and incubated overnight ( 4 ° C ) with primary antibodies ( FasL , Fas , caspases 8, 3 , PARP1 , p53 , E2F1 , PUMA , ATF6 , XBP1 , CREB2 , GADD153 , BECN1 , LC3B , p62 ) using indicated dilutions ( Table S1 ) .