sentence: DIP media was changed every 24 h , and the differentiation process was complete by 72 h. For iCRT14 treatment , undifferentiated HC11 cells were treated with 5 µ M iCRT14 ( Tocris ) for 48 h. Levels of β - casein ( Santa Cruz sc-17971 1:1000 ) , total β - catenin ( Cell Signaling # 9582 1:5000 ) , and phospho - β - catenin ( Cell Signaling # 5651 1:1000 ) were assessed by immunoblot .