sentence: At these culturing conditions ∼ 80 – 90 % of the L. major parasites exhibited an aflagellated rounded morphology and displayed similar characteristics of previously described axenic amastigotes including , but not limited to doubling time ( i.e ., ∼ 24 h ) , clustered growth patterns , agglutination response to PNA lectin , protease activity and protein expression profiles [ 46 ] – [ 49 ] .