sentence: With plants , attempts also have been made by electron microscopy and live-cell imaging to observe the Golgi after BFA removal ( Ritzenthaler et al ., 2002 ; Langhans et al ., 2007 ; Schoberer et al ., 2010 ) , but time-dependent regeneration processes of the Golgi stacks have not been sufficiently described.In the present study , we performed multicolor time-lapse live-cell imaging with short intervals , which enabled us to analyze detailed behaviors of multiple Golgi membrane proteins in the same cells with the passage of time .