sentence: Pol II promoter ( CMV ) based constructs ( pEZX-MR04 ) overexpressing precursor mmu-miR-151 and hsa-miR-124-3 were obtained from Gene Copoeia.Plasmids expressing FLAG tagged human Argonautes ( Ago2 , Ago1 ) and EGFP were obtained from Add gene ( plasmids 10822 , 10820 , 10825 ) .