sentence: The overexpression of either the wild-type or non phosphorylatable Cav-1 was carried out by transfection of pCAGGS / Cav-1 ( provided by F. Galbiati , University of Pittsburgh , USA ) or pEGFPN1 / Cav-1 ( Y14F ) ( provided by D. Maggi , University of Genova , Italy ) constructs , respectively .