sentence: The exact amino acid composition of SUR1-MRP1 chimeric constructs and mutants were as follows : SUR1S1M = SUR1 ( M1-V1313 ) + MRP1 ( V1261-F1341 ) + SUR1 ( R1394-K1582 ) ; SUR1S2M = SUR1 ( M1-P1336 ) + MRP1 ( P1284-L1300 ) + SUR1 ( V1352-K1582 ) ; SUR1S3M = SUR1 with mutations N1338S , I1345V , S1351C , and V1352L ; SUR1S4M = SUR1 with mutations K1337S , D1341Q , K1344R , Q1346E , and Q1348R ; SUR1 = SUR1 with mutations Q1342V , I1347F , and L1350Y , SUR1S2M = SUR1S2M with mutations V1290Q , F1295I , and Y1298L , SUR1 = Q1342I , I1347I , and L1350I , SUR1 = Q1342A , I1347A , and L1350A , SUR1 = Q1342G , I1347G , and L1350G , SUR2A = E1305I , I1310I , and L1313I , SUR2A = E1305A , I1310A , and L1313A , SUR2A = E1305G , I1310G , and L1313G.After amplification and linearization , plasmid DNAs were transcribed in vitro using the T7 mMessage mMachine Kit ( Life Technologies , Saint Aubin , France ) to produce cRNAs for later Xenopus oocyte microinjection .