sentence: Genomic DNA was enriched from two mgn mutant females by region specific extraction ( RSE ) [ 22 ] , [ 72 ] for two overlapping regions : 1 ) A 319.8 kb interval on chromosome 19 ( 32,557,347 – 32,877,103 ; Zv7 / danRer5 ) where the molecular lesion associated with the mgn phenotype was predicted to be based on meiotic mapping ; and 2 ) A 613.9 kb interval on chromosome 19 ( 32,326,938 – 32,942,969 ; Zv7 / danRer5 ) that extended the 319.8 kb region on both sides of macf1 to examine flanking genes and identify SNPs .