sentence: The pBtoxis ( NC_010076 ) was used as reference for the SNPs analysis of pT0124-4 , pT0131-4 , pT0137-4 , and pT0139-4 using Geneious v 10.1.3 , “ Find SNPs / InDels ” tool , with minimum coverage of 10 , minimum variance frequence 0.75 .