sentence: Genomes were annotated using DNAMaster v.5.23.6 ( cobamide2.pitt.edu ) and PECAAN v.20210526 ( discover.kbrinsgd.org ) with analysis by Glimmer v.3.02 , Genemark v.3.25 , NCBI BLAST v.2.9.0 , tRNAscan-SE v.2.0 , Aragorn v.1.2.38 , and HHpred v.3.2.0 informing decisions about gene location and function prediction during annotation .