sentence: The membrane was blocked with 5 % skim milk and incubated at 4 ° C overnight with the following primary antibodies : phospho ‐ adenosine monophosphate protein kinase ( AMPK ) ‐ T172 , AMPK , p ‐ Raptor ‐ S792 , Raptor , p ‐ mTOR ‐ S2448 , mTOR , p ‐ p70S6K ‐ T389 , p70S6K , β ‐ actin ( 1:1000 ; Cell Signaling Technology , Danvers , MA , USA ) and caspase ‐ 3 ( 1:200 ; Santa Cruz Biotechnology , Santa Cruz , CA , USA ) .