sentence: The cDNAs of SINV C ( nt 7647-8449 ) , 6KE1 ( nt 9900-11474 ) , E3E2 ( nt 8449-9900 ) , 6kE1 Δ TMD ( nt 9900-11283 ) , E1 Δ FD ( nt 10693-11474 ) , and 6K ( nt 9900-10064 ) with EcoRI adapter sequences were obtained via reverse transcription-polymerase chain reaction ( RT-PCR ) of the SINV genomic RNA .