sentence: Trypomastigote form Trypanosoma cruzi Y strain ( 1.5 × 10 cells / mL ) was added into wells containing BMMOs in two different ways : ( Treatment 1 ) trypomastigotes and triazole-linked compounds at a final concentration of 250 μ M were plated at the same time ; and ( Treatment 2 ) trypomastigotes and triazole-linked compounds at a final concentration of 250 μ M were first pre-incubated for 1 h , then the parasites were washed to remove free compound and were plated to the wells containing BMMOs .