sentence: To generate the RPL22 overexpression constructs , the respective RPL22 ORFs were PCR-amplified from genomic DNA from intronless strains ( KAY61 strain and primers FN12 + FN13 for RPL22A ; KAY67 strain and primers FN14 + FN15 for RPL22B ) , digested with NcoI / BamHI , and cloned into plasmid pVTU260 digested with the same enzymes .