sentence: Expression of recombinant proteins in Escherichia coli BL21-Rosetta-DE3 was induced with 0.1 mM isopropyl β - d-thiogalactoside for 4 h at 37 ° C ( ZZ-Kuk , ZZ-Kuk-C567S , ZZ-Kuk - Δ N185 , and ZZ-Kuk - Δ N437 ) or for 4 h at 18 ° C ( GFP-LaminDm0 Δ N , GFP-NLS-C-term , and GFP-NLS-CaaX ) .