sentence: Macrophages derived from bone marrow ( BMMOs ) were obtained as described previously.43 Briefly , total bone marrow cells were cultured in RPMI 1640 medium ( Sigma – Aldrich ) , supplemented with 10 % Fetal Bovine Serum ( Cutilab ) and 30 % L-929 cell-conditioned media at 37 ° C under 5 % CO atmosphere .