sentence: Triggered by the observations of Ralle et al. ( 2004 ) and Prufert et al. ( 2004 ) , we have investigated the relation of farnesylated NM proteins with the NM and the phospholipid bilayer of the membrane , focusing on the C-terminal part containing the CaaX motif.From our structure – function analysis in the fly embryo , we confirmed that both the coiled coil motif and the CaaX motif are required for Kuk NM localization and activity , as reported previously ( Brandt et al ., 2006 ) .