Update dash_plotly_QC_scRNA.py

dash_plotly_QC_scRNA.py

@@ -405,7 +405,8 @@ def update_graph_and_pie_chart(batch_chosen, s_chosen, g2m_chosen, condition1_ch
     expression_means = dff_long.lazy().group_by(["batch", "Gene"]).agg(pl.mean("Mean expression")).collect()
     
     # Calculate the percentage total expressed
-    dff_long2 = dff_pre.melt(id_vars="batch", variable_name="Gene").group_by(pl.col(["batch","Gene","value"])).len()
+    dff_long1 = dff_pre.melt(id_vars="batch", variable_name="Gene")
+    dff_long2 = dff_long1.group_by(pl.col(["batch","Gene","value"])).len()
     dff_long3 = dff_long2.filter(value = 0).group_by(["batch", "Gene"]).agg(pl.sum("len").alias("total"))
     dff_long4 = dff_long2.group_by(["batch", "Gene"]).agg(pl.sum("len").alias("total"))
     dff_5 = dff_long4.join(dff_long3, on=["batch","Gene"], how="outer")