import os
import gradio as gr
from prediction import run_image_prediction
import torch
import torchvision.transforms as T
from celle.utils import process_image
from PIL import Image
from matplotlib import pyplot as plt
from celle_main import instantiate_from_config
from huggingface_hub import hf_hub_download
from omegaconf import OmegaConf

class model:
    def __init__(self):
        self.model = None
        self.model_name = None

    def gradio_demo(self, model_name, sequence_input, nucleus_image, protein_image):
        device = torch.device("cuda" if torch.cuda.is_available() else "cpu")
        
        if self.model_name != model_name:
            self.model_name = model_name
            model_ckpt_path = hf_hub_download(repo_id=f"HuangLab/{model_name}", filename="model.ckpt")
            model_config_path = hf_hub_download(repo_id=f"HuangLab/{model_name}", filename="config.yaml")
            hf_hub_download(repo_id=f"HuangLab/{model_name}", filename="nucleus_vqgan.yaml")
            hf_hub_download(repo_id=f"HuangLab/{model_name}", filename="threshold_vqgan.yaml")
            
            
            # Load model config and set ckpt_path if not provided in config
            config = OmegaConf.load(model_config_path)
            if config["model"]["params"]["ckpt_path"] is None:
                config["model"]["params"]["ckpt_path"] = model_ckpt_path

            # Set condition_model_path and vqgan_model_path to None
            config["model"]["params"]["condition_model_path"] = None
            config["model"]["params"]["vqgan_model_path"] = None
            
            base_path = os.getcwd()

            os.chdir(os.path.dirname(model_ckpt_path))

            # Instantiate model from config and move to device
            self.model = instantiate_from_config(config.model).to(device)
            self.model = torch.compile(self.model,mode='max-autotune')
            
            os.chdir(base_path)


        if "Finetuned" in model_name:
            dataset = "OpenCell"

        else:
            dataset = "HPA"
        
        to_tensor = T.ToTensor()
        
        nucleus_tensor = to_tensor(nucleus_image)

        if protein_image:
            protein_tensor = to_tensor(protein_image)
            stacked_images = torch.stack([nucleus_tensor, protein_tensor], dim=0)
            processed_images = process_image(stacked_images, dataset)
            nucleus_image = processed_images[0].unsqueeze(0)
            protein_image = processed_images[1].unsqueeze(0)
            protein_image = protein_image > 0
            protein_image = 1.0 * protein_image
        else:
            protein_image = torch.ones((256, 256))

        threshold, heatmap = run_image_prediction(
            sequence_input=sequence_input,
            nucleus_image=nucleus_image,
            model=self.model,
            device=device,
        )

        # Plot the heatmap
        plt.imshow(heatmap.cpu(), cmap="rainbow", interpolation="bicubic")
        plt.axis("off")

        # Save the plot to a temporary file
        plt.savefig("temp.png", bbox_inches="tight", dpi=256)

        # Open the temporary file as a PIL image
        heatmap = Image.open("temp.png")

        return (
            T.ToPILImage()(nucleus_image[0, 0]),
            T.ToPILImage()(protein_image),
            T.ToPILImage()(threshold),
            heatmap,
        )

base_class = model()

with gr.Blocks(theme='gradio/soft') as demo:
    gr.Markdown("## Inputs")
    gr.Markdown("Select the prediction model. **Note the first run may take ~1-2 minutes, but will take 2-3 seconds afterwards.**")
    gr.Markdown(
        "```CELL-E_2_HPA_480``` is a good general purpose model for various cell types using ICC-IF."
    )
    gr.Markdown(
        "```CELL-E_2_HPA_Finetuned_480``` is finetuned on OpenCell and is good more live-cell predictions on HEK cells."
    )
    with gr.Row():
        model_name = gr.Dropdown(
            ["CELL-E_2_HPA_480", "CELL-E_2_HPA_Finetuned_480"],
            value="CELL-E_2_HPA_480",
            label="Model Name",
        )
    with gr.Row():
        gr.Markdown(
            "Input the desired amino acid sequence. GFP is shown below by default."
        )

    with gr.Row():
        sequence_input = gr.Textbox(
            value="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK",
            label="Sequence",
        )
    with gr.Row():
        gr.Markdown(
            "Uploading a nucleus image is necessary. A random crop of 256 x 256 will be applied if larger. We provide default images in [images](https://huggingface.co/spaces/HuangLab/CELL-E_2/tree/main/images)"
        )
        gr.Markdown("The protein image is optional and is just used for display.")

    with gr.Row().style(equal_height=True):
        nucleus_image = gr.Image(
            type="pil",
            label="Nucleus Image",
            image_mode="L",
        )

        protein_image = gr.Image(type="pil", label="Protein Image (Optional)")

    with gr.Row():
        gr.Markdown("## Outputs")

    with gr.Row():
        gr.Markdown("Image predictions are show below.")

    with gr.Row().style(equal_height=True):
        nucleus_image_crop = gr.Image(type="pil", label="Nucleus Image", image_mode="L")

        protein_threshold_image = gr.Image(
            type="pil", label="Protein Threshold Image", image_mode="L"
        )

        predicted_threshold_image = gr.Image(
            type="pil", label="Predicted Threshold image", image_mode="L"
        )

        predicted_heatmap = gr.Image(type="pil", label="Predicted Heatmap")
    with gr.Row():
        button = gr.Button("Run Model")

        inputs = [model_name, sequence_input, nucleus_image, protein_image]

        outputs = [
            nucleus_image_crop,
            protein_threshold_image,
            predicted_threshold_image,
            predicted_heatmap,
        ]

        button.click(base_class.gradio_demo, inputs, outputs)

demo.launch(enable_queue=True)