updated

cas9on.py

@@ -106,11 +106,15 @@ def process_gene(gene_symbol, model_path):
     if transcripts:
         for transcript in transcripts:
             transcript_id = transcript['id']
+            chr = transcript.get('seq_region_name', 'unknown')
+            start = transcript.get('start', 0)
+            strand = transcript.get('strand', 'unknown')
             gene_sequence = fetch_ensembl_sequence(transcript_id)
+
             if gene_sequence:
-                gRNA_sites = find_crispr_targets(gene_sequence)
+                gRNA_sites = find_crispr_targets(gene_sequence, chr, start, strand)
                 if gRNA_sites:
-                    formatted_data = format_prediction_output(gRNA_sites, transcript_id, model_path)
+                    formatted_data = format_prediction_output(gRNA_sites, model_path)
                     all_data.extend(formatted_data)
 
     return all_data