fix

cas9on.py

@@ -208,19 +208,18 @@ def create_bigwig(df, bigwig_path):
         df = pd.DataFrame(df, columns=["Chr", "Start Pos", "End Pos", "Strand", "Transcript", "Exon", "Target", "gRNA", "Prediction"])
 
     # Calculate chromosome sizes as the maximum end position per chromosome
-    # Ensure the sizes are integers
     chrom_sizes = df.groupby('Chr')['End Pos'].max().astype(int).to_dict()
 
     with pyBigWig.open(bigwig_path, "w") as bw:
-        # Add chromosome sizes to the header, ensuring sizes are integers
+        # Add chromosome sizes to the header
         bw.addHeader([(chr, size) for chr, size in chrom_sizes.items()])
 
-        # Iterate over unique chromosomes and add entries for each
+        # Iterate over the DataFrame and add entries for each chromosome
         for chrom in df['Chr'].unique():
             chrom_df = df[df['Chr'] == chrom]
-            bw.addEntries(
-                chrom,
-                chrom_df['Start Pos'].astype(int).tolist(),
-                ends=chrom_df['End Pos'].astype(int).tolist(),
-                values=chrom_df['Prediction'].astype(float).tolist()
-            )
+            starts = chrom_df['Start Pos'].astype(int).tolist()
+            ends = chrom_df['End Pos'].astype(int).tolist()
+            values = chrom_df['Prediction'].astype(float).tolist()
+
+            if starts and ends and values:
+                bw.addEntries(chrom, starts, ends=ends, values=values)