#ref: https://huggingface.co/blog/AmelieSchreiber/esmbind
import gradio as gr

import os
# os.environ["CUDA_VISIBLE_DEVICES"] = "0"
#import wandb
import numpy as np
import torch
import torch.nn as nn
import pickle
import xml.etree.ElementTree as ET
from datetime import datetime
from sklearn.model_selection import train_test_split
from sklearn.utils.class_weight import compute_class_weight
from sklearn.metrics import (
    accuracy_score, 
    precision_recall_fscore_support, 
    roc_auc_score, 
    matthews_corrcoef
)
from transformers import (
    AutoModelForTokenClassification,
    AutoTokenizer,
    DataCollatorForTokenClassification,
    TrainingArguments,
    Trainer
)

from peft import PeftModel

from datasets import Dataset
from accelerate import Accelerator
# Imports specific to the custom peft lora model
from peft import get_peft_config, PeftModel, PeftConfig, get_peft_model, LoraConfig, TaskType

from plot_pdb import plot_struc

def suggest(option):
    if option == "Plastic degradation protein":
        suggestion = "MGSSHHHHHHSSGLVPRGSHMRGPNPTAASLEASAGPFTVRSFTVSRPSGYGAGTVYYPTNAGGTVGAIAIVPGYTARQSSIKWWGPRLASHGFVVITIDTNSTLDQPSSRSSQQMAALRQVASLNGTSSSPIYGKVDTARMGVMGWSMGGGGSLISAANNPSLKAAAPQAPWDSSTNFSSVTVPTLIFACENDSIAPVNSSALPIYDSMSRNAKQFLEINGGSHSCANSGNSNQALIGKKGVAWMKRFMDNDTRYSTFACENPNSTRVSDFRTANCSLEDPAANKARKEAELAAATAEQ"
    elif option == "Default protein":
        #suggestion = "MAPLRKTYVLKLYVAGNTPNSVRALKTLNNILEKEFKGVYALKVIDVLKNPQLAEEDKILATPTLAKVLPPPVRRIIGDLSNREKVLIGLDLLYEEIGDQAEDDLGLE"
        suggestion = "MAVPETRPNHTIYINNLNEKIKKDELKKSLHAIFSRFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGT" 
    elif option == "Antifreeze protein":
        suggestion = "QCTGGADCTSCTGACTGCGNCPNAVTCTNSQHCVKANTCTGSTDCNTAQTCTNSKDCFEANTCTDSTNCYKATACTNSSGCPGH"
    elif option == "AI Generated protein":
        suggestion = "MSGMKKLYEYTVTTLDEFLEKLKEFILNTSKDKIYKLTITNPKLIKDIGKAIAKAAEIADVDPKEIEEMIKAVEENELTKLVITIEQTDDKYVIKVELENEDGLVHSFEIYFKNKEEMEKFLELLEKLISKLSGS"
    elif option == "7-bladed propeller fold":
        suggestion = "VKLAGNSSLCPINGWAVYSKDNSIRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTVKDRSPHRTLMSCPVGEAPSPYNSRFESVAWSASACHDGTSWLTIGISGPDNGAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTVMTDGPSNGQASYKIFKMEKGKVVKSVELDAPNYHYEECSCYPNAGEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGVFGDNPRPNDGTGSCGPVSSNGAYGVKGFSFKYGNGVWIGRTKSTNSRSGFEMIWDPNGWTETDSSFSVKQDIVAITDWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKESTIWTSGSSISFCGVNSDTVGWSWPDGAELPFTIDK"
    else:
        suggestion = ""
    return suggestion
    
# Helper Functions and Data Preparation
def truncate_labels(labels, max_length):
    """Truncate labels to the specified max_length."""
    return [label[:max_length] for label in labels]

def compute_metrics(p):
    """Compute metrics for evaluation."""
    predictions, labels = p
    predictions = np.argmax(predictions, axis=2)
    
    # Remove padding (-100 labels)
    predictions = predictions[labels != -100].flatten()
    labels = labels[labels != -100].flatten()
    
    # Compute accuracy
    accuracy = accuracy_score(labels, predictions)
    
    # Compute precision, recall, F1 score, and AUC
    precision, recall, f1, _ = precision_recall_fscore_support(labels, predictions, average='binary')
    auc = roc_auc_score(labels, predictions)
    
    # Compute MCC
    mcc = matthews_corrcoef(labels, predictions) 
    
    return {'accuracy': accuracy, 'precision': precision, 'recall': recall, 'f1': f1, 'auc': auc, 'mcc': mcc} 

def compute_loss(model, inputs): 
    """Custom compute_loss function."""
    logits = model(**inputs).logits
    labels = inputs["labels"]
    loss_fct = nn.CrossEntropyLoss(weight=class_weights)
    active_loss = inputs["attention_mask"].view(-1) == 1
    active_logits = logits.view(-1, model.config.num_labels)
    active_labels = torch.where(
        active_loss, labels.view(-1), torch.tensor(loss_fct.ignore_index).type_as(labels)
    )
    loss = loss_fct(active_logits, active_labels)
    return loss

# Define Custom Trainer Class
# Since we are using class weights, due to the imbalance between non-binding residues and binding residues, we will need a custom weighted trainer.
class WeightedTrainer(Trainer):
    def compute_loss(self, model, inputs, return_outputs=False): 
        outputs = model(**inputs)
        loss = compute_loss(model, inputs) 
        return (loss, outputs) if return_outputs else loss

# Predict binding site with finetuned PEFT model
def predict_bind(base_model_path,PEFT_model_path,input_seq):
    # Load the model
    base_model = AutoModelForTokenClassification.from_pretrained(base_model_path)
    loaded_model = PeftModel.from_pretrained(base_model, PEFT_model_path)

    # Ensure the model is in evaluation mode
    loaded_model.eval()
    
    # Tokenization
    tokenizer = AutoTokenizer.from_pretrained(base_model_path) 

    # Tokenize the sequence
    inputs = tokenizer(input_seq, return_tensors="pt", truncation=True, max_length=1024, padding='max_length')

    # Run the model
    with torch.no_grad():
        logits = loaded_model(**inputs).logits

    # Get predictions
    tokens = tokenizer.convert_ids_to_tokens(inputs["input_ids"][0])  # Convert input ids back to tokens
    predictions = torch.argmax(logits, dim=2)

    binding_site=[]
    pos = 0
    # Print the predicted labels for each token
    for token, prediction in zip(tokens, predictions[0].numpy()):
        if token not in ['<pad>', '<cls>', '<eos>']:
            pos += 1
            print((pos, token, id2label[prediction]))
            if prediction == 1:
                print((pos, token, id2label[prediction]))
                binding_site.append([pos, token, id2label[prediction]])
                
    return binding_site

# fine-tuning function
def train_function_no_sweeps(base_model_path):   #, train_dataset, test_dataset):
    
    # Set the LoRA config
    config = {
        "lora_alpha": 1, #try 0.5, 1, 2, ..., 16
        "lora_dropout": 0.2,
        "lr": 5.701568055793089e-04,
        "lr_scheduler_type": "cosine",
        "max_grad_norm": 0.5,
        "num_train_epochs": 1,  #3, jw 20240628
        "per_device_train_batch_size": 12,
        "r": 2,
        "weight_decay": 0.2,
        # Add other hyperparameters as needed
    }

    base_model = AutoModelForTokenClassification.from_pretrained(base_model_path, num_labels=len(id2label), id2label=id2label, label2id=label2id)
    
    # Tokenization
    tokenizer = AutoTokenizer.from_pretrained(base_model_path) #("facebook/esm2_t12_35M_UR50D")
    
    train_tokenized = tokenizer(train_sequences, padding=True, truncation=True, max_length=max_sequence_length, return_tensors="pt", is_split_into_words=False)
    test_tokenized = tokenizer(test_sequences, padding=True, truncation=True, max_length=max_sequence_length, return_tensors="pt", is_split_into_words=False)
    
    train_dataset = Dataset.from_dict({k: v for k, v in train_tokenized.items()}).add_column("labels", train_labels)
    test_dataset = Dataset.from_dict({k: v for k, v in test_tokenized.items()}).add_column("labels", test_labels)
    
    # Convert the model into a PeftModel
    peft_config = LoraConfig(
        task_type=TaskType.TOKEN_CLS, 
        inference_mode=False, 
        r=config["r"], 
        lora_alpha=config["lora_alpha"], 
        target_modules=["query", "key", "value"], # also try "dense_h_to_4h" and "dense_4h_to_h"
        lora_dropout=config["lora_dropout"], 
        bias="none" # or "all" or "lora_only" 
    )
    base_model = get_peft_model(base_model, peft_config)

    # Use the accelerator
    base_model = accelerator.prepare(base_model)
    train_dataset = accelerator.prepare(train_dataset)
    test_dataset = accelerator.prepare(test_dataset)

    model_name_base = base_model_path.split("/")[1]
    timestamp = datetime.now().strftime('%Y-%m-%d_%H')
    save_path = f"{model_name_base}-lora-binding-sites_{timestamp}"
    
    # Training setup
    training_args = TrainingArguments(
        output_dir=save_path, #f"{model_name_base}-lora-binding-sites_{timestamp}",
        learning_rate=config["lr"],
        lr_scheduler_type=config["lr_scheduler_type"],
        gradient_accumulation_steps=1,
        max_grad_norm=config["max_grad_norm"],
        per_device_train_batch_size=config["per_device_train_batch_size"],
        per_device_eval_batch_size=config["per_device_train_batch_size"],
        num_train_epochs=config["num_train_epochs"],
        weight_decay=config["weight_decay"],
        evaluation_strategy="epoch",
        save_strategy="epoch",
        load_best_model_at_end=True,
        metric_for_best_model="f1",
        greater_is_better=True,
        push_to_hub=True,  #jw 20240701  False,
        logging_dir=None,
        logging_first_step=False,
        logging_steps=200,
        save_total_limit=7,
        no_cuda=False,
        seed=8893,
        fp16=True,
        #report_to='wandb'
        report_to=None,
        hub_token = HF_TOKEN, #jw 20240701
    )

    # Initialize Trainer
    trainer = WeightedTrainer(
        model=base_model,
        args=training_args,
        train_dataset=train_dataset,
        eval_dataset=test_dataset,
        tokenizer=tokenizer,
        data_collator=DataCollatorForTokenClassification(tokenizer=tokenizer),
        compute_metrics=compute_metrics,
        
    )

    # Train and Save Model
    trainer.train()

    return save_path

# Constants & Globals
HF_TOKEN = os.environ.get("HF_token")
print("HF_TOKEN:",HF_TOKEN)

MODEL_OPTIONS = [
    "facebook/esm2_t6_8M_UR50D",
    "facebook/esm2_t12_35M_UR50D",
    "facebook/esm2_t33_650M_UR50D",
]  # models users can choose from

PEFT_MODEL_OPTIONS = [
    "wangjin2000/esm2_t6_8M-lora-binding-sites_2024-07-02_09-26-54",
    "AmelieSchreiber/esm2_t12_35M_lora_binding_sites_v2_cp3",
]  # finetuned models 


# Load the data from pickle files (replace with your local paths)
with open("./datasets/train_sequences_chunked_by_family.pkl", "rb") as f:
    train_sequences = pickle.load(f)

with open("./datasets/test_sequences_chunked_by_family.pkl", "rb") as f:
    test_sequences = pickle.load(f)

with open("./datasets/train_labels_chunked_by_family.pkl", "rb") as f:
    train_labels = pickle.load(f)

with open("./datasets/test_labels_chunked_by_family.pkl", "rb") as f:
    test_labels = pickle.load(f)

max_sequence_length = 1000

# Directly truncate the entire list of labels
train_labels = truncate_labels(train_labels, max_sequence_length)
test_labels = truncate_labels(test_labels, max_sequence_length)

# Compute Class Weights
classes = [0, 1]  
flat_train_labels = [label for sublist in train_labels for label in sublist]
class_weights = compute_class_weight(class_weight='balanced', classes=classes, y=flat_train_labels)
accelerator = Accelerator()
class_weights = torch.tensor(class_weights, dtype=torch.float32).to(accelerator.device)

# Define labels and model
id2label = {0: "No binding site", 1: "Binding site"}
label2id = {v: k for k, v in id2label.items()}

'''
# debug result
dubug_result = saved_path  #predictions  #class_weights
'''

demo = gr.Blocks(title="DEMO FOR ESM2Bind")

with demo:
    gr.Markdown("# DEMO FOR ESM2Bind")
    #gr.Textbox(dubug_result)
    
    with gr.Column():
        gr.Markdown("## Select a base model and a corresponding PEFT finetune model")

        with gr.Row():   
            with gr.Column(scale=5, variant="compact"):
                base_model_name = gr.Dropdown(
                    choices=MODEL_OPTIONS,
                    value=MODEL_OPTIONS[0],
                    label="Base Model Name",
                    interactive = True,
                )  
                PEFT_model_name = gr.Dropdown(
                    choices=PEFT_MODEL_OPTIONS,
                    value=PEFT_MODEL_OPTIONS[0],
                    label="PEFT Model Name",
                    interactive = True,
                )
            with gr.Column(scale=5, variant="compact"): 
                    name = gr.Dropdown(
                        label="Choose a Sample Protein", 
                        value="Default protein", 
                        choices=["Default protein", "Antifreeze protein", "Plastic degradation protein",  "AI Generated protein", "7-bladed propeller fold", "custom"]
                    )
        gr.Markdown(
                "## Predict binding site and Plot structure for selected protein sequence:"
                )
        with gr.Row():
            with gr.Column(variant="compact", scale = 8):  
                input_seq = gr.Textbox(
                    lines=1,
                    max_lines=12,
                    label="Protein sequency to be predicted:",
                    value="MAVPETRPNHTIYINNLNEKIKKDELKKSLHAIFSRFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGT",
                    placeholder="Paste your protein sequence here...",
                    interactive = True,
                ) 
                text_pos = gr.Textbox(
                    lines=1,
                    max_lines=12,
                    label="Sequency Position:",
                    placeholder=
                    "012345678911234567892123456789312345678941234567895123456789612345678971234567898123456789912345678901234567891123456789",
                    interactive=False,
                )
            with gr.Column(variant="compact", scale = 2):
                    predict_btn = gr.Button(
                        value="Predict binding site", 
                        interactive=True, 
                        variant="primary",
                    )
                    plot_struc_btn = gr.Button(value = "Plot ESMFold Predicted Structure ", variant="primary")
        with gr.Row():  
            with gr.Column(variant="compact", scale = 5):
                output_text = gr.Textbox(
                    lines=1,
                    max_lines=12,
                    label="Output",
                    placeholder="Output",
                )   
            with gr.Column(variant="compact", scale = 5):  
                finetune_button = gr.Button(
                    value="Finetune Pre-trained Model", 
                    interactive=True, 
                    variant="primary",
                )
        with gr.Row(): 
            output_viewer = gr.HTML()
            output_file = gr.File(
                label="Download as Text File",
                file_count="single",
                type="filepath",  
                interactive=False,
            )  
            
    # select protein sample
    name.change(fn=suggest, inputs=name, outputs=input_seq)   

    # "Predict binding site" actions
    predict_btn.click(
        fn = predict_bind,
        inputs=[base_model_name,PEFT_model_name,input_seq], 
        outputs = [output_text],
    )
    
    # "Finetune Pre-trained Model" actions
    finetune_button.click(
        fn = train_function_no_sweeps,
        inputs=[base_model_name],
        outputs = [output_text],
    )
           
    # plot protein structure
    plot_struc_btn.click(fn=plot_struc, inputs=input_seq, outputs=[output_file, output_viewer])

    
demo.launch()