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Indirect and Sandwich ELISA 
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Western Blotting 
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Solid-Phase Synthesis of Proteins 
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Introduction to Nucleic Acids 
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Composition of Nucleic Acids 
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Nucleosides and Nucleotides
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Watson-Crick Model of DNA
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Meselson and Stahl Experiment 
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Melting and Annealing of DNA 
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Stem-Loop Structure of RNA 
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DNA Polymerase and Catalysis of Phosphodiester Bond
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Circular DNA, RNA Genes and Viruses 
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Types of RNA 
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RNA Polymerase 
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Promoter and Termination Sites 
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Central Dogma and Genetic Code 
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Start and Stop Codons 
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Exons and Introns of Eukaryotic mRNA
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Introduction to Biotechnology
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Restriction Enzymes and Palindromic Sequences 
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Southern and Northern Blotting
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Sanger Sequencing of DNA 
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Sanger Sequencing of DNA (Part II) 
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Amplifying DNA with Polymerase Chain Reaction 
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Plasmids and Recombinant DNA technology 
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Lambda Phages as Vectors 
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Building and Screening Genomic Libraries 
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Cassette Mutagenesis and Gene Deletions 
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Site-Directed Mutagenesis
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Synthesizing cDNA with Reverse Transcriptase 
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Gene Transfection 
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Heme Group of Hemoglobin and Myoglobin 
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Oxygen Binding Curve for Myoglobin and Hemoglobin
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Hemoglobin vs Myoglobin as Oxygen Carrier 
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T-state and R-state of Hemoglobin
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Concerted and Sequential Model for Hemoglobin
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Effect of 2,3-BPG on Hemoglobin 
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Fetal Hemoglobin and 2,3 BPG 
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The Bohr Effect and Hemoglobin
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The Bohr Effect and Hemoglobin (Part II) 
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Transport of Carbon Dioxide and Chloride Shift 
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Haldane Effect 
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High Altitude and 2,3 BPG
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Alpha Hemoglobin and Stabilizing Protein 
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Sickle-Cell Anemia 
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Properties of Enzymes 
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Enzymes’ Effect on Activation Energy and Free Energy 
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Gibbs Free Energy and Spontaneity
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Enzymes Stabilize Transition State 
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Properties of Active Sites, Lock-and-Key Model and Induced Fit Model 
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Effect of Enzymes on Rate Law and Rate Constant 
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Effect of Enzymes on Rate Law and Rate Constant (Part II) 
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Derivation of Michaelis-Menten Equation
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Derivation of Michaelis Menten-Equation Part II 
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Michaelis menten Equation 
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Michaelis Constant 
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Maximal Velocity and Turnover Number of Enzymes 
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Catalytic Efficiency of Enzymes 
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Catalytic Efficiency of Enzymes Part II 
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Sequential and Ping Pong Reactions 
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Mechanisms of Enzyme Catalysis 
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Introduction to Proteases
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Chymotrypsin and Covalent Catalysis 
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Reaction Mechanism of Chymotrypsin
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Specificity of Serine Proteases
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Cysteine, Apsratyl and metalloproteases
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Carbonic Anhyrdase
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NMP Kinases 
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Irreversible and Reversible Inhibition
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Irreversibile and Reversible Inhibition Part II 
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Enzyme Kinetics of Reversible Inhibition
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Enzyme Kinetics of Reversible Inhibition (Part II) 
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Lineweaver Burke Plot and Reversible Inhibition
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Lineweaver Burke Plot and REversible Inhibition Part II 
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Group Specific Affinity Labels and Suicide Inhibitors 
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Transition State Analogs and Catalytic Antibodies 
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Structure and Function of Penicillin 
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Enzyme Regulation 
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ATCase Allosteric Regulation
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Structure of ATCase 
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Structure of ATCase Part II
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Cooperatively and Allosteric Effectors of ATCase 
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Covalent Modification and Phosphorylation 
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Covalent Modification and Phosphorylation Part II 
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Protein Kinase A 
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Proteolytic Activation 
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Proteolytic Activation of Digestive Enzymes 
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Inhibition of Digestive Enzymes 
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Inhibition of Digestive Enzymes 
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Activation of Coagulation Cascade 
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Factor VIII and Hemophilia A 
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Inhibition of Coagulation Cascade 
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Isozymes 
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Introduction to Carbohydrates 
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Aldoses, Ketoses Fischer Projections and Epimers 
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Cyclic Form of Carbohydrates 
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Stability of Glucose Anomie’s 
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Modification of Carbohydrates 
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Disaccharides (maltose, lactose, and sucrose) 
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Polysaccharides 
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