Enzyme
stringlengths 4
59
| Organism
stringlengths 1
70
| Substrate
stringlengths 1
86
| km
float64 0
1.4M
⌀ | Unit_Km
stringclasses 65
values | Kcat
stringlengths 1
16
| Unit_Kcat
stringclasses 134
values | Kcat/Km
stringlengths 1
21
| Unit_Kcat/Km
stringclasses 335
values | Commentary[Temp]
stringclasses 165
values | Commentary[pH]
stringclasses 225
values | Commentary[Mutant]
stringlengths 1
93
| Commentary[Cosubstrate]
stringlengths 1
89
| pubmed id
float64 1.5M
35.5M
⌀ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
DmsABC | Escherichia coli | pyridine N-oxide | 0.18 | mM | 190 | s^-1 | 1100 | s^-1mM^-1 | M147I | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 7.6 | s^-1 | M147I | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.11 | mM | 370 | s^-1 | 3300 | s^-1mM^-1 | T148S | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.024 | mM | 96 | s^-1 | 4000 | s^-1mM^-1 | T148S | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 21 | s^-1 | T148S | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.076 | mM | 68 | s^-1 | 900 | s^-1mM^-1 | R149C | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.12 | mM | 95 | s^-1 | 810 | s^-1mM^-1 | R149C | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 12 | s^-1 | R149C | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.038 | mM | 14 | s^-1 | 370 | s^-1mM^-1 | G167N | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.076 | mM | 99 | s^-1 | 1300 | s^-1mM^-1 | G167N | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 1.9 | s^-1 | G167N | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | null | mM | 23 | s^-1 | 23300 | s^-1mM^-1 | A178Q | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | null | mM | 10.0 | s^-1 | >10000 | s^-1mM^-1 | A178Q | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 1.9 | s^-1 | A178Q | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.019 | mM | 19 | s^-1 | 1000 | s^-1mM^-1 | Q179I | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.034 | mM | 36 | s^-1 | 1100 | s^-1mM^-1 | Q179I | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 2.2 | s^-1 | Q179I | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.039 | mM | 210 | s^-1 | 5500 | s^-1mM^-1 | A181T | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.24 | mM | 940 | s^-1 | 4000 | s^-1mM^-1 | A181T | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 39 | s^-1 | A181T | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.033 | mM | 110 | s^-1 | 3400 | s^-1mM^-1 | G190V | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.12 | mM | 260 | s^-1 | 2100 | s^-1mM^-1 | G190V | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 16 | s^-1 | G190V | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.038 | mM | 60 | s^-1 | 1600 | s^-1mM^-1 | G190D | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.15 | mM | 190 | s^-1 | 1200 | s^-1mM^-1 | G190D | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 10 | s^-1 | G190D | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.028 | mM | 47 | s^-1 | 1600 | s^-1mM^-1 | W191G | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.048 | mM | 99 | s^-1 | 2100 | s^-1mM^-1 | W191G | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 8.2 | s^-1 | W191G | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 1.1 | mM | 55 | s^-1 | 50 | s^-1mM^-1 | R217Q | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 3.8 | mM | 120 | s^-1 | 33 | s^-1mM^-1 | R217Q | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 6.3 | s^-1 | R217Q | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.019 | mM | 24 | s^-1 | 1300 | s^-1mM^-1 | W357Y | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.028 | mM | 33 | s^-1 | 1200 | s^-1mM^-1 | W357Y | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 7.2 | s^-1 | W357Y | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.024 | mM | 46 | s^-1 | 1900 | s^-1mM^-1 | W357C | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.095 | mM | 100 | s^-1 | 1100 | s^-1mM^-1 | W357C | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | Me2SO | null | 4.6 | s^-1 | W357C | DMNH2 | 9,492,325 |
|||||
DmsABC | Escherichia coli | Me2SO | 0.023 | mM | 17 | s^-1 | 760 | s^-1mM^-1 | W357F | BVH^+ | 9,492,325 |
||
DmsABC | Escherichia coli | pyridine N-oxide | 0.048 | mM | 41 | s^-1 | 850 | s^-1mM^-1 | W357F | BVH^+ | 9,492,325 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | 9,10-phenanthrenequinone | 2 | μM | 171 | min^-1 | 86 | min^-1 μM^-1 | 25 | 6.0 | Wild-type | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 9,10-phenanthrenequinone | 14 | μM | 87 | min^-1 | 6.2 | min^-1 μM^-1 | 25 | 6.0 | Y55F | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 9,10-phenanthrenequinone | 26 | μM | 87 | min^-1 | 3.3 | min^-1 μM^-1 | 25 | 6.0 | Y55S | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 9,10-phenanthrenequinone | 56 | μM | 0.7 | min^-1 | 0.01 | min^-1 μM^-1 | 25 | 6.0 | D50N | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 9,10-phenanthrenequinone | 12 | μM | 0.9 | min^-1 | 0.08 | min^-1 μM^-1 | 25 | 6.0 | D50E | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 9,10-phenanthrenequinone | 44 | μM | 2.3 | min^-1 | 0.05 | min^-1 μM^-1 | 25 | 6.0 | H117A | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | Acenaphthenequinone | 3 | μM | 60 | min^-1 | 20 | min^-1 μM^-1 | 25 | 6.0 | Wild-type | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | Acenaphthenequinone | 5 | μM | 30 | min^-1 | 6.0 | min^-1 μM^-1 | 25 | 6.0 | Y55F | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | Acenaphthenequinone | 7 | μM | 30 | min^-1 | 4.3 | min^-1 μM^-1 | 25 | 6.0 | Y55S | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 1-Acenaphthenone | 1,000 | μM | 0.3 | min^-1 | 0.0003 | min^-1 μM^-1 | 25 | 6.0 | Wild-type | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 1-Acenaphthenone | 1,000 | μM | 0.3 | min^-1 | 0.0003 | min^-1 μM^-1 | 25 | 6.0 | Y55F | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 1-Acenaphthenone | 1,000 | μM | 0.3 | min^-1 | 0.0003 | min^-1 μM^-1 | 25 | 6.0 | Y55S | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | (+)(1S)-Camphorquinone | 1,000 | μM | 0.6 | min^-1 | 0.0006 | min^-1 μM^-1 | 25 | 6.0 | Wild-type | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | (+)(1S)-Camphorquinone | 1,000 | μM | 0.6 | min^-1 | 0.0006 | min^-1 μM^-1 | 25 | 6.0 | Y55F | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | (+)(1S)-Camphorquinone | 1,000 | μM | 0.6 | min^-1 | 0.0006 | min^-1 μM^-1 | 25 | 6.0 | Y55S | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 1-(R,S)-Acenaphthenol | 1,000 | μM | 0.3 | min^-1 | 0.0003 | min^-1 μM^-1 | 25 | 9.0 | Wild-type | NADP+ (2.3 mM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 1-(R,S)-Acenaphthenol | 1,000 | μM | 0.3 | min^-1 | 0.0003 | min^-1 μM^-1 | 25 | 9.0 | Y55F | NADP+ (2.3 mM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 1-(R,S)-Acenaphthenol | 1,000 | μM | 0.3 | min^-1 | 0.0003 | min^-1 μM^-1 | 25 | 9.0 | Y55S | NADP+ (2.3 mM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | Androsterone | 3 | μM | 30 | min^-1 | 10 | min^-1 μM^-1 | 25 | 9.0 | Wild-type | NADP+ (2.3 mM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | Androsterone | 1,000 | μM | 0.3 | min^-1 | 0.0003 | min^-1 μM^-1 | 25 | 9.0 | Y55F | NADP+ (2.3 mM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | Androsterone | 1,000 | μM | 0.3 | min^-1 | 0.0003 | min^-1 μM^-1 | 25 | 9.0 | Y55S | NADP+ (2.3 mM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | Androstanedione | 3 | μM | 30 | min^-1 | 10 | min^-1 μM^-1 | 25 | 6.0 | Wild-type | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | Androstanedione | 1,000 | μM | 0.3 | min^-1 | 0.0003 | min^-1 μM^-1 | 25 | 6.0 | Y55F | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | Androstanedione | 1,000 | μM | 0.3 | min^-1 | 0.0003 | min^-1 μM^-1 | 25 | 6.0 | Y55S | NADPH (180 μM) | 9,692,994 |
3α-hydroxysteroid dehydrogenase | Rat liver | 9,10-phenanthrenequinone | 40 | μM | 4.36 | μmol/min/mg | 25 | 6.0 | Wild-type | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | 9,10-phenanthrenequinone | 40 | μM | 1.78 | μmol/min/mg | 25 | 6.0 | Y55F | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | 9,10-phenanthrenequinone | 40 | μM | 1.55 | μmol/min/mg | 25 | 6.0 | Y55S | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | acenaphthenequinone | 100 | μM | 3.79 | μmol/min/mg | 25 | 6.0 | Wild-type | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | acenaphthenequinone | 100 | μM | 0.61 | μmol/min/mg | 25 | 6.0 | Y55F | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | acenaphthenequinone | 100 | μM | 0.59 | μmol/min/mg | 25 | 6.0 | Y55S | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | (R,S)-camphorquinone | 800 | μM | 2.54 | μmol/min/mg | 25 | 6.0 | Wild-type | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | (R,S)-camphorquinone | 800 | μM | 0.003 | μmol/min/mg | 25 | 6.0 | Y55F | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | (R,S)-camphorquinone | 800 | μM | 0.02 | μmol/min/mg | 25 | 6.0 | Y55S | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | naphthalene-1,2-dione | 40 | μM | 0.263 | μmol/min/mg | 25 | 6.0 | Wild-type | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | menadione | 40 | μM | 0.024 | μmol/min/mg | 25 | 6.0 | Wild-type | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | naphthalene-1,4-dione | 40 | μM | 0.047 | μmol/min/mg | 25 | 6.0 | Wild-type | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | 1-acenaphthenone | 400 | μM | 0.06 | μmol/min/mg | 25 | 6.0 | Wild-type | NADPH | 9,692,994 |
||
3α-hydroxysteroid dehydrogenase | Rat liver | 1-acenaphthenol | 400 | μM | 0.15 | μmol/min/mg | 25 | 9.0 | Wild-type | NADP+ | 9,692,994 |
||
β-glycosidase | Sulfolobus solfataricus | 2,4-DNP-β-Glc | 0.17 | mM | 275 | s^-1 | 1617 | s^-1mM^-1 | 65°C | 6.5 | Wild type | None | 9,860,840 |
β-glycosidase | Sulfolobus solfataricus | 2,4-DNP-β-Glc | 1.64 | mM | 428 | s^-1 | 261 | s^-1mM^-1 | 65°C | 6.5 | Wild type | 2 M sodium azide | 9,860,840 |
β-glycosidase | Sulfolobus solfataricus | 2,4-DNP-β-Glc | 0.61 | mM | 367 | s^-1 | 602 | s^-1mM^-1 | 65°C | 6.5 | Wild type | 2 M sodium formate | 9,860,840 |
β-glycosidase | Sulfolobus solfataricus | 2,4-DNP-β-Glc | 1.21 | mM | 9.6 | s^-1 | 8 | s^-1mM^-1 | 65°C | 6.5 | Glu387Ala | 2 M sodium azide | 9,860,840 |
β-glycosidase | Sulfolobus solfataricus | 2,4-DNP-β-Glc | 0.18 | mM | 110 | s^-1 | 611 | s^-1mM^-1 | 65°C | 6.5 | Glu387Gly | 2 M sodium azide | 9,860,840 |
β-glycosidase | Sulfolobus solfataricus | 2,4-DNP-β-Glc | 0.13 | mM | 42 | s^-1 | 323 | s^-1mM^-1 | 65°C | 6.5 | Glu387Gly | 2 M sodium formate | 9,860,840 |
β-glycosidase | Sulfolobus solfataricus | 2-NP-β-Glc | 1.01 | mM | 538 | s^-1 | 533 | s^-1mM^-1 | 65°C | 6.5 | Wild type | None | 9,860,840 |
β-glycosidase | Sulfolobus solfataricus | 2-NP-β-Glc | 0.98 | mM | 480 | s^-1 | 490 | s^-1mM^-1 | 65°C | 6.5 | Wild type | 2 M sodium azide | 9,860,840 |
β-glycosidase | Sulfolobus solfataricus | 2-NP-β-Glc | 0.5 | mM | 425 | s^-1 | 850 | s^-1mM^-1 | 65°C | 6.5 | Wild type | 2 M sodium formate | 9,860,840 |
β-glycosidase | Sulfolobus solfataricus | 2-NP-β-Glc | 0.8 | mM | 15 | s^-1 | 19 | s^-1mM^-1 | 65°C | 6.5 | Glu387Gly | 2 M sodium azide | 9,860,840 |