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33123853 | Experiences of using an activating spinal orthosis in women with osteoporosis and back pain in primary care. | Women with osteoporosis and back pain took part in focus group interviews and described their experiences of using and handling an activating spinal orthosis. The women described the back orthosis as being like a "close friend", a support in everyday life and a reminder to maintain a good posture. The purpose of this study was to describe and gain a deeper understanding of the views of older women with osteoporosis and back pain seeking primary care regarding their use and handling of an activating spinal orthosis. We chose a qualitative method whereby information was gathered via focus group interviews and analysed using inductive content analysis. Women who previously participated in a randomised controlled trial and wore an activating spinal orthosis for 6 months were asked. Out of 31 women, 18 agreed to participate. Five focus group interviews were conducted. The analysis resulted in an overall theme in which the experiences of wearing the spinal orthosis were described as follows: "A well-adapted spinal orthosis could develop into a long-lasting friendship that provided support and help in daily life". The overall theme was based on three main categories: impact on daily life, individual adaptation and personal relationship. The main categories were well differentiated from each other but had an interdependency. All three categories involved cases in which the spinal orthosis was perceived as relieving symptoms and making daily life easier, as well as when it was perceived as being hard to manage and provided no symptom relief. In older women with osteoporosis and back pain, an activating spinal orthosis could be perceived as being a "close friend" and a support in everyday life. To facilitate acceptance of the spinal orthosis, it was important for it to be well adapted and for follow-ups to be carried out regularly. | 2020 Oct 29 | Archives of osteoporosis | No DOI | [
{
"LastName": "Alin",
"FirstName": "Christina Kaijser",
"Affiliation": "Department of Neurobiology, Care Sciences and Society, Division of Family Medicine and Primary Care, Karolinska Institutet, Solna, Sweden. christina.kaijser.alin@ki.se."
},
{
"LastName": "Frisendahl",
"FirstName": "Nathalie",
"Affiliation": "Department of Neurobiology, Care Sciences and Society, Division of Physiotherapy, Solna, Sweden."
},
{
"LastName": "Kronhed",
"FirstName": "Ann-Charlotte Grahn",
"Affiliation": "Rehab Väst, Local Health Care Services in the West of Östergötland, Mjölby, Sweden."
},
{
"LastName": "Salminen",
"FirstName": "Helena",
"Affiliation": "Department of Neurobiology, Care Sciences and Society, Division of Family Medicine and Primary Care, Karolinska Institutet, Solna, Sweden."
}
] | No |
36065632 | Mega-sized pericentromeric blocks of simple telomeric repeats and their variants reveal patterns of chromosome evolution in ancient Cycadales genomes. | Simple telomeric repeats composed of six to seven iterating nucleotide units are important sequences typically found at the ends of chromosomes. Here we analyzed their abundance and homogeneity in 42 gymnosperm (29 newly sequenced), 29 angiosperm (one newly sequenced), and eight bryophytes using bioinformatics, conventional cytogenetic and molecular biology approaches to explore their diversity across land plants. We found more than 10 000-fold variation in the amounts of telomeric repeats among the investigated taxa. Repeat abundance was positively correlated with increasing intragenomic sequence heterogeneity and occurrence at non-telomeric positions, but there was no correlation with genome size. The highest abundance/heterogeneity was found in the gymnosperm genus Cycas (Cycadaceae), in which megabase-sized blocks of telomeric repeats (i.e., billions of copies) were identified. Fluorescent in situ hybridization experiments using variant-specific probes revealed canonical Arabidopsis-type telomeric TTTAGGG repeats at chromosome ends, while pericentromeric blocks comprised at least four major telomeric variants with decreasing abundance: TTTAGGG>TTCAGGG >TTTAAGG>TTCAAGG. Such a diversity of repeats was not found in the sister cycad family Zamiaceae or in any other species analyzed. Using immunocytochemistry, we showed that the pericentromeric blocks of telomeric repeats overlapped with histone H3 serine 10 phosphorylation signals. We show that species of Cycas have amplified their telomeric repeats in centromeric and telomeric positions on telocentric chromosomes to extraordinary high levels. The ancestral chromosome number reconstruction suggests their occurrence is unlikely to be the product of ancient Robertsonian chromosome fusions. We speculate as to how the observed chromosome dynamics may be associated with the diversification of cycads. | 2022 Nov | The Plant journal : for cell and molecular biology | No DOI | [
{
"LastName": "Vozárová",
"FirstName": "Radka",
"Affiliation": "Department of Molecular Epigenetics, Institute of Biophysics, Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65, Brno, Czech Republic."
},
{
"LastName": "Wang",
"FirstName": "Wencai",
"Affiliation": "Science and Technology Innovation Centre, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China."
},
{
"LastName": "Lunerová",
"FirstName": "Jana",
"Affiliation": "Department of Molecular Epigenetics, Institute of Biophysics, Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65, Brno, Czech Republic."
},
{
"LastName": "Shao",
"FirstName": "Fengqing",
"Affiliation": "Science and Technology Innovation Centre, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China."
},
{
"LastName": "Pellicer",
"FirstName": "Jaume",
"Affiliation": "Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, UK."
},
{
"LastName": "Leitch",
"FirstName": "Ilia J",
"Affiliation": "Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, UK."
},
{
"LastName": "Leitch",
"FirstName": "Andrew R",
"Affiliation": "School of Biological and Chemical Sciences, Queen Mary University of London, London, E1 4NS, UK."
},
{
"LastName": "Kovařík",
"FirstName": "Aleš",
"Affiliation": "Department of Molecular Epigenetics, Institute of Biophysics, Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65, Brno, Czech Republic."
}
] | No |
29363717 | Inhibition of microRNA-19b promotes ovarian granulosa cell proliferation by targeting IGF-1 in polycystic ovary syndrome. | The purpose of the present study was to investigate the functional role of microRNA (miR)-19b in polycystic ovary syndrome (PCOS) and try to elucidate its underlying mechanisms. Expression of miR‑19b and insulin‑like growth factor 1 (IGF-1) was examined in ovarian cortexes [(from 18 women with PCOS and 10 who did not have PCOS (non‑PCOS)] and KGN cells. Cell proliferation assays (cell viability and colony formation assay) were performed following overexpression or inhibition of miR‑19b and IGF‑1 or following insulin treatment in KGN cells. Expression levels of the cell cycle-associated protein cyclin D1 and cyclin‑dependent kinase (CDK) 1 were analyzed following overexpression or inhibition of miR-19b and IGF-1. Potential miR‑19b targets were identified by bioinformatics. Luciferase assay, reverse transcription‑quantitative polymerase chain reaction and western blotting were performed to determine whether IGF‑1 was a target of miR‑19b. miR‑19b expression was significantly decreased in the PCOS ovarian cortex and KGN cells and its identified target, IGF‑1, was upregulated. miR‑19b overexpression inhibited cell proliferation at G2/M phrase. Overexpression of IGF‑1 promoted cell viability and colony formation ability in KGN cells. The expression of cyclin D1 and CDK1 was statistically increased by inhibition of miR‑19b and overexpression of IGF‑1. High concentrations of insulin decreased levels of miR‑19b, stimulated KGN cell proliferation, and elevated IGF‑1 levels. Inhibition of miR‑19b promoted ovarian granulosa cell proliferation by targeting IGF‑1 in PCOS. Insulin decreased the expression levels of miR‑19b and stimulated cell proliferation. The present study suggested that overexpression of miR‑19b may be a potential therapeutic approach for PCOS. | 2018 Apr | Molecular medicine reports | No DOI | [
{
"LastName": "Zhong",
"FirstName": "Zhuohui",
"Affiliation": "Department of General Gynaecology, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong 510623, P.R. China."
},
{
"LastName": "Li",
"FirstName": "Fang",
"Affiliation": "Department of Obstetrics and Gynecology, Eighth People's Hospital of Guangzhou, Guangzhou, Guangdong 510060, P.R. China."
},
{
"LastName": "Li",
"FirstName": "Yingying",
"Affiliation": "Department of General Gynaecology, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong 510623, P.R. China."
},
{
"LastName": "Qin",
"FirstName": "Shuang",
"Affiliation": "Department of General Gynaecology, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong 510623, P.R. China."
},
{
"LastName": "Wen",
"FirstName": "Canliang",
"Affiliation": "Department of General Gynaecology, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong 510623, P.R. China."
},
{
"LastName": "Fu",
"FirstName": "Yiyuan",
"Affiliation": "Department of General Gynaecology, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong 510623, P.R. China."
},
{
"LastName": "Xiao",
"FirstName": "Qing",
"Affiliation": "Department of General Gynaecology, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong 510623, P.R. China."
}
] | Yes |
27127137 | A Prospective Observational Study of Adoptive Immunotherapy for Cancer Using Zoledronate-Activated Killer (ZAK) Cells - An Analysis for Patients with Incurable Pancreatic Cancer. | Adoptive immunotherapy (AIT) using autologous zoledronate-activated killer (ZAK) cells has been performed for developing a novel modality of cancer treatment. In this study, data series from incurable pancreatic cancer were analyzed. Patients were treated with AIT using intravenous administration of ZAK cells every 3 to 4 weeks in combination with standard chemotherapy and possible clinical benefits were examined. Seventy-five patients were treated. A median overall survival (OS) time of 6.7 months was achieved for all patients and 13.1 months for those treated 5 times or more, that increased to 14.6 and 18.3 months, respectively, when the previous treatment period of chemotherapy alone was included in the analysis. The disease control rate was 58.5 %. Multivariate regression analysis showed a significant positive correlation between the survival and baseline value of lymphocyte percentage in white blood cell counts (p=0.031). The data suggest that AIT using ZAK cells in combination with chemotherapy is safe and feasible and may be effective in prolonging survival for patients with incurable pancreatic cancer. The lymphocyte percentage at baseline may be a good biomarker for predicting the survival benefit of ZAK cell AIT. | 2016 May | Anticancer research | No DOI | [
{
"LastName": "Yamaguchi",
"FirstName": "Yoshiyuki",
"Affiliation": "Department of Clinical Oncology, Kawasaki Medical School and Hospital, Kurashiki, Okayama, Japan shogo@med.kawasaki-m.ac.jp."
},
{
"LastName": "Katata",
"FirstName": "Yousuke",
"Affiliation": "Department of Clinical Oncology, Kawasaki Medical School and Hospital, Kurashiki, Okayama, Japan."
},
{
"LastName": "Okawaki",
"FirstName": "Makoto",
"Affiliation": "Department of Clinical Oncology, Kawasaki Medical School and Hospital, Kurashiki, Okayama, Japan."
},
{
"LastName": "Sawaki",
"FirstName": "Akira",
"Affiliation": "Department of Clinical Oncology, Kawasaki Medical School and Hospital, Kurashiki, Okayama, Japan."
},
{
"LastName": "Yamamura",
"FirstName": "Masahiro",
"Affiliation": "Department of Clinical Oncology, Kawasaki Medical School and Hospital, Kurashiki, Okayama, Japan."
}
] | Yes |
36057977 | Long-read HiFi sequencing of NUDT15: Phased full-gene haplotyping and pharmacogenomic allele discovery. | To determine the phase of NUDT15 sequence variants for more comprehensive star (*) allele diplotyping, we developed a novel long-read single-molecule real-time HiFi amplicon sequencing method. A 10.5 kb NUDT15 amplicon assay was validated using reference material positive controls and additional samples for specimen type and blinded accuracy assessment. Triplicate NUDT15 HiFi sequencing of two reference material samples had nonreference genotype concordances of >99.9%, indicating that the assay is robust. Notably, short-read genome sequencing of a subset of samples was unable to determine the phase of star (*) allele-defining NUDT15 variants, resulting in ambiguous diplotype results. In contrast, long-read HiFi sequencing phased all variants across the NUDT15 amplicons, including a *2/*9 diplotype that previously was characterized as *1/*2 in the 1000 Genomes Project v3 data set. Assay throughput was also tested using 8.5 kb amplicons from 100 Ashkenazi Jewish individuals, which identified a novel NUDT15 *1 suballele (c.-121G>A) and a rare likely deleterious coding variant (p.Pro129Arg). Both novel alleles were Sanger confirmed and assigned as *1.007 and *20, respectively, by the PharmVar Consortium. Taken together, NUDT15 HiFi amplicon sequencing is an innovative method for phased full-gene characterization and novel allele discovery, which could improve NUDT15 pharmacogenomic testing and subsequent phenotype prediction. | 2022 Nov | Human mutation | No DOI | [
{
"LastName": "Scott",
"FirstName": "Erick R",
"Affiliation": "Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA."
},
{
"LastName": "Yang",
"FirstName": "Yao",
"Affiliation": "Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA."
},
{
"LastName": "Botton",
"FirstName": "Mariana R",
"Affiliation": "Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA."
},
{
"LastName": "Seki",
"FirstName": "Yoshinori",
"Affiliation": "Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA."
},
{
"LastName": "Hoshitsuki",
"FirstName": "Keito",
"Affiliation": "School of Pharmacy, University of Pittsburgh, Pennsylvania, Pittsburgh, USA."
},
{
"LastName": "Harting",
"FirstName": "John",
"Affiliation": "Pacific Biosciences, Menlo Park, California, USA."
},
{
"LastName": "Baybayan",
"FirstName": "Primo",
"Affiliation": "Pacific Biosciences, Menlo Park, California, USA."
},
{
"LastName": "Cody",
"FirstName": "Neal",
"Affiliation": "Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA."
},
{
"LastName": "Nicoletti",
"FirstName": "Paola",
"Affiliation": "Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA."
},
{
"LastName": "Moriyama",
"FirstName": "Takaya",
"Affiliation": "Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, Tennessee, USA."
},
{
"LastName": "Chakraborty",
"FirstName": "Shreyasee",
"Affiliation": "Pacific Biosciences, Menlo Park, California, USA."
},
{
"LastName": "Yang",
"FirstName": "Jun J",
"Affiliation": "Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, Tennessee, USA."
},
{
"LastName": "Edelmann",
"FirstName": "Lisa",
"Affiliation": "Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA."
},
{
"LastName": "Schadt",
"FirstName": "Eric E",
"Affiliation": "Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA."
},
{
"LastName": "Korlach",
"FirstName": "Jonas",
"Affiliation": "Pacific Biosciences, Menlo Park, California, USA."
},
{
"LastName": "Scott",
"FirstName": "Stuart A",
"Affiliation": "Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA."
}
] | No |
36868858 | Multiple and Dissociable Effects of Sensory History on Working-Memory Performance. | Behavioral reports of sensory information are biased by stimulus history. The nature and direction of such serial-dependence biases can differ between experimental settings; both attractive and repulsive biases toward previous stimuli have been observed. How and when these biases arise in the human brain remains largely unexplored. They could occur either via a change in sensory processing itself and/or during postperceptual processes such as maintenance or decision-making. To address this, we tested 20 participants (11 female) and analyzed behavioral and magnetoencephalographic (MEG) data from a working-memory task in which participants were sequentially presented with two randomly oriented gratings, one of which was cued for recall at the end of the trial. Behavioral responses showed evidence for two distinct biases: (1) a within-trial repulsive bias away from the previously encoded orientation on the same trial, and (2) a between-trial attractive bias toward the task-relevant orientation on the previous trial. Multivariate classification of stimulus orientation revealed that neural representations during stimulus encoding were biased away from the previous grating orientation, regardless of whether we considered the within-trial or between-trial prior orientation, despite opposite effects on behavior. These results suggest that repulsive biases occur at the level of sensory processing and can be overridden at postperceptual stages to result in attractive biases in behavior.SIGNIFICANCE STATEMENT Recent experience biases behavioral reports of sensory information, possibly capitalizing on the temporal regularity in our environment. It is still unclear at what stage of stimulus processing such serial biases arise. Here, we recorded behavior and neurophysiological [magnetoencephalographic (MEG)] data to test whether neural activity patterns during early sensory processing show the same biases seen in participants' reports. In a working-memory task that produced multiple biases in behavior, responses were biased toward previous targets, but away from more recent stimuli. Neural activity patterns were uniformly biased away from all previously relevant items. Our results contradict proposals that all serial biases arise at an early sensory processing stage. Instead, neural activity exhibited mostly adaptation-like responses to recent stimuli. | 2023 Apr 12 | The Journal of neuroscience : the official journal of the Society for Neuroscience | No DOI | [
{
"LastName": "Hajonides",
"FirstName": "Jasper E",
"Affiliation": "Oxford Centre for Human Brain Activity, Wellcome Centre for Integrative Neuroimaging, University of Oxford, Oxford, OX3 7JX, United Kingdom."
},
{
"LastName": "van Ede",
"FirstName": "Freek",
"Affiliation": "Department of Applied and Experimental Psychology, Vrije Universiteit Amsterdam, 1081 BT, Amsterdam, Netherlands."
},
{
"LastName": "Stokes",
"FirstName": "Mark G",
"Affiliation": "Oxford Centre for Human Brain Activity, Wellcome Centre for Integrative Neuroimaging, University of Oxford, Oxford, OX3 7JX, United Kingdom."
},
{
"LastName": "Nobre",
"FirstName": "Anna C",
"Affiliation": "Oxford Centre for Human Brain Activity, Wellcome Centre for Integrative Neuroimaging, University of Oxford, Oxford, OX3 7JX, United Kingdom."
},
{
"LastName": "Myers",
"FirstName": "Nicholas E",
"Affiliation": "School of Psychology, University of Nottingham, Nottingham, NG7 2RD, United Kingdom nicholas.myers@nottingham.ac.uk."
}
] | No |
22028792 | Plexin-B1 activates NF-κB and IL-8 to promote a pro-angiogenic response in endothelial cells. | The semaphorins and their receptors, the plexins, are proteins related to c-Met and the scatter factors that have been implicated in an expanding signal transduction network involving co-receptors, RhoA and Ras activation and deactivation, and phosphorylation events. Our previous work has demonstrated that Semaphorin 4D (Sema4D) acts through its receptor, Plexin-B1, on endothelial cells to promote angiogenesis in a RhoA and Akt-dependent manner. Since NF-κB has been linked to promotion of angiogenesis and can be activated by Akt in some contexts, we wanted to examine NF-κB in Sema4D treated cells to determine if there was biological significance for the pro-angiogenic phenotype observed in endothelium. Using RNA interference techniques, gel shifts and NF-κB reporter assays, we demonstrated NF-κB translocation to the nucleus in Sema4D treated endothelial cells occurring downstream of Plexin-B1. This response was necessary for endothelial cell migration and capillary tube formation and protected endothelial cells against apoptosis as well, but had no effect on cell proliferation. We dissected Plexin-B1 signaling with chimeric receptor constructs and discovered that the ability to activate NF-κB was dependent upon Plexin-B1 acting through Rho and Akt, but did not involve its role as a Ras inhibitor. Indeed, inhibition of Rho by C3 toxin and Akt by LY294002 blocked Sema4D-mediated endothelial cell migration and tubulogenesis. We also observed that Sema4D treatment of endothelial cells induced production of the NF-κB downstream target IL-8, a response necessary for angiogenesis. Finally, we could show through co-immunofluorescence for p65 and CD31 that Sema4D produced by tumor xenografts in nude mice activated NF-κB in vessels of the tumor stroma. These findings provide evidence that Sema4D/Plexin-B1-mediated NF-κB activation and IL-8 production is critical in the generation a pro-angiogenic phenotype in endothelial cells and suggests a new therapeutic target for the anti-angiogenic treatment of some cancers. | 2011 | PloS one | No DOI | [
{
"LastName": "Yang",
"FirstName": "Ying-Hua",
"Affiliation": "Department of Oncology and Diagnostic Sciences, University of Maryland Dental School, Baltimore, Maryland, United States of America."
},
{
"LastName": "Zhou",
"FirstName": "Hua",
"Affiliation": ""
},
{
"LastName": "Binmadi",
"FirstName": "Nada O",
"Affiliation": ""
},
{
"LastName": "Proia",
"FirstName": "Patrizia",
"Affiliation": ""
},
{
"LastName": "Basile",
"FirstName": "John R",
"Affiliation": ""
}
] | Yes |
26793144 | Temporal Dynamics of the Integration of Intention and Outcome in Harmful and Helpful Moral Judgment. | The ability to integrate the moral intention information with the outcome of an action plays a crucial role in mature moral judgment. Functional magnetic resonance imaging (fMRI) studies implicated that both prefrontal and temporo-parietal cortices are involved in moral intention and outcome processing. Here, we used the event-related potentials (ERPs) technique to investigate the temporal dynamics of the processing of the integration between intention and outcome information in harmful and helpful moral judgment. In two experiments, participants were asked to make moral judgments for agents who produced either negative/neutral outcomes with harmful/neutral intentions (harmful judgment) or positive/neutral outcomes with helpful/neutral intentions (helpful judgment). Significant ERP differences between attempted and successful actions over prefrontal and bilateral temporo-parietal regions were found in both harmful and helpful moral judgment, which suggest a possible time course of the integration processing in the brain, starting from the right temporo-parietal area (N180) to the left temporo-parietal area (N250), then the prefrontal area (FSW) and the right temporo-parietal area (TP450 and TPSW) again. These results highlighted the fast moral intuition reaction and the late integration processing over the right temporo-parietal area. | 2015 | Frontiers in psychology | No DOI | [
{
"LastName": "Gan",
"FirstName": "Tian",
"Affiliation": "State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal UniversityBeijing, China; Department of Psychology, Zhejiang Sci-Tech UniversityHangzhou, China."
},
{
"LastName": "Lu",
"FirstName": "Xiaping",
"Affiliation": "State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal UniversityBeijing, China; Center for Collaboration and Innovation in Brain and Learning Sciences, Beijing Normal UniversityBeijing, China."
},
{
"LastName": "Li",
"FirstName": "Wanqing",
"Affiliation": "State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal UniversityBeijing, China; Center for Collaboration and Innovation in Brain and Learning Sciences, Beijing Normal UniversityBeijing, China."
},
{
"LastName": "Gui",
"FirstName": "Danyang",
"Affiliation": "State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal UniversityBeijing, China; Center for Collaboration and Innovation in Brain and Learning Sciences, Beijing Normal UniversityBeijing, China."
},
{
"LastName": "Tang",
"FirstName": "Honghong",
"Affiliation": "State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal UniversityBeijing, China; Center for Collaboration and Innovation in Brain and Learning Sciences, Beijing Normal UniversityBeijing, China."
},
{
"LastName": "Mai",
"FirstName": "Xiaoqin",
"Affiliation": "Department of Psychology, Renmin University of China Beijing, China."
},
{
"LastName": "Liu",
"FirstName": "Chao",
"Affiliation": "State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal UniversityBeijing, China; Center for Collaboration and Innovation in Brain and Learning Sciences, Beijing Normal UniversityBeijing, China."
},
{
"LastName": "Luo",
"FirstName": "Yue-Jia",
"Affiliation": "Institute of Affective and Social Neuroscience, Shenzhen UniversityShenzhen, China; Collaborative Innovation Center of Sichuan for Elder Care and Health, Chengdu Medical CollegeChengdu, China."
}
] | No |
22917544 | Quantitative analysis of the mutagenic potential of 1-aminopyrene-DNA adduct bypass catalyzed by Y-family DNA polymerases. | N-(Deoxyguanosin-8-yl)-1-aminopyrene (dG(AP)) is the predominant nitro polyaromatic hydrocarbon product generated from the air pollutant 1-nitropyrene reacting with DNA. Previous studies have shown that dG(AP) induces genetic mutations in bacterial and mammalian cells. One potential source of these mutations is the error-prone bypass of dG(AP) lesions catalyzed by the low-fidelity Y-family DNA polymerases. To provide a comparative analysis of the mutagenic potential of the translesion DNA synthesis (TLS) of dG(AP), we employed short oligonucleotide sequencing assays (SOSAs) with the model Y-family DNA polymerase from Sulfolobus solfataricus, DNA Polymerase IV (Dpo4), and the human Y-family DNA polymerases eta (hPolη), kappa (hPolκ), and iota (hPolι). Relative to undamaged DNA, all four enzymes generated far more mutations (base deletions, insertions, and substitutions) with a DNA template containing a site-specifically placed dG(AP). Opposite dG(AP) and at an immediate downstream template position, the most frequent mutations made by the three human enzymes were base deletions and the most frequent base substitutions were dAs for all enzymes. Based on the SOSA data, Dpo4 was the least error-prone Y-family DNA polymerase among the four enzymes during the TLS of dG(AP). Among the three human Y-family enzymes, hPolκ made the fewest mutations at all template positions except opposite the lesion site. hPolκ was significantly less error-prone than hPolι and hPolη during the extension of dG(AP) bypass products. Interestingly, the most frequent mutations created by hPolι at all template positions were base deletions. Although hRev1, the fourth human Y-family enzyme, could not extend dG(AP) bypass products in our standing start assays, it preferentially incorporated dCTP opposite the bulky lesion. Collectively, these mutagenic profiles suggest that hPolk and hRev1 are the most suitable human Y-family DNA polymerases to perform TLS of dG(AP) in humans. | 2012 Sep 1 | Mutation research | No DOI | [
{
"LastName": "Sherrer",
"FirstName": "Shanen M",
"Affiliation": "Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA."
},
{
"LastName": "Taggart",
"FirstName": "David J",
"Affiliation": ""
},
{
"LastName": "Pack",
"FirstName": "Lindsey R",
"Affiliation": ""
},
{
"LastName": "Malik",
"FirstName": "Chanchal K",
"Affiliation": ""
},
{
"LastName": "Basu",
"FirstName": "Ashis K",
"Affiliation": ""
},
{
"LastName": "Suo",
"FirstName": "Zucai",
"Affiliation": ""
}
] | No |
34796915 | Roburic acid attenuates osteoclastogenesis and bone resorption by targeting RANKL-induced intracellular signaling pathways. | Excessive activity of osteoclasts contributes to skeletal diseases such as osteoporosis and osteolysis. However, current drugs targeting osteoclast have various deficiencies, placing natural compounds as substitutions of great potential. Roburic acid (RA) is a triterpenoid exacted from Radix Gentianae Macrophyllae, which exhibits inhibitory effects on inflammation and oxidation. By employing an in vitro osteoclastogenesis model, this study investigates the effects and mechanisms of RA on intracellular signaling induced by receptor activator of nuclear factor-κB ligand (RANKL). As expected, RA at a concentration scope from 1 to 10 μM dampened the osteoclast differentiation of bone marrow macrophages (BMMs) but without cell toxicity. Interestingly, RA showed no effect on osteoblastogenesis in vitro. Furthermore, RA mitigated F-actin ring formation, hydroxyapatite resorption, and gene expression in osteoclasts. Mechanistically, RA suppressed TNF receptor-associated factor 6 (TRAF6), the crucial adaptor protein following RANKL-RANK binding. On the one hand, RA downregulated the nuclear factor-κB (NF-κB) activity, extracellular regulated protein kinases (ERK) phosphorylation, and calcium oscillations. On the other hand, RA upregulated the antioxidative response element (ARE) response and the protein expression of heme oxygenase (HO)-1. These upstream alterations eventually led to the suppression of the nuclear factor of activated T cells 1 (NFATc1) activity and the expression of proteins involved in osteoclastogenesis and bone resorption. Furthermore, by using an ovariectomized (OVX) mice model, RA was found to have therapeutic effects against bone loss. On account of these findings, RA could be used to restrain osteoclasts for treating osteoporosis and other osteolytic diseases. | 2022 Mar | Journal of cellular physiology | No DOI | [
{
"LastName": "Wang",
"FirstName": "Gang",
"Affiliation": "Department of Orthopaedics, First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China."
},
{
"LastName": "Chen",
"FirstName": "Kai",
"Affiliation": "School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia."
},
{
"LastName": "Ma",
"FirstName": "Chao",
"Affiliation": "Guangzhou University of Chinese Medicine, Guangzhou, China."
},
{
"LastName": "Wang",
"FirstName": "Chao",
"Affiliation": "School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia."
},
{
"LastName": "Chen",
"FirstName": "Delong",
"Affiliation": "School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia."
},
{
"LastName": "He",
"FirstName": "Jianbo",
"Affiliation": "School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia."
},
{
"LastName": "Liu",
"FirstName": "Yuhao",
"Affiliation": "Department of Orthopaedics, First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China."
},
{
"LastName": "Jiang",
"FirstName": "Tao",
"Affiliation": "Department of Orthopaedics, Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, China."
},
{
"LastName": "Yuan",
"FirstName": "Jinbo",
"Affiliation": "School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia."
},
{
"LastName": "Chen",
"FirstName": "Leilei",
"Affiliation": "Department of Orthopaedics, Third Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China."
},
{
"LastName": "He",
"FirstName": "Wei",
"Affiliation": "Department of Orthopaedics, Third Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China."
},
{
"LastName": "Xu",
"FirstName": "Jiake",
"Affiliation": "School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia."
}
] | No |
26897589 | Rock porosity quantification by dual-energy X-ray computed microtomography. | Porous media investigation by X-ray microtomography allows obtaining valuable quantitative and qualitative information, while preserving sample integrity. Modern X-ray nanotomography or Synchrotron radiation systems may distinguish structures sized only hundreds of nanometers. However, pores sized less than a few microns (microporosity) may be undetectable due to the system's spatial resolution and noise in microfocus sources, compromising the quality of the measurement. In this study a dual-energy methodology was developed to generate density-based images from two scans made at two different voltages (80kV and 130KV) with a microfocus bench-top microtomography system. The images obtained were quantized in 256 gray levels, where the lowest value (zero) corresponded to voids and the highest value (255) corresponded to the densest regions mapped. From density images and single energy images, porosity was evaluated and compared. Results indicate that density images present better results than single energy images when both are compared with porosity obtained by the helium injection method. In addition, images acquired in dual-energy show good agreement with the sample's real density values. | 2016 Apr | Micron (Oxford, England : 1993) | No DOI | [
{
"LastName": "Teles",
"FirstName": "A P",
"Affiliation": "Nuclear Engineering Program, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: ateles@con.ufrj.br."
},
{
"LastName": "Lima",
"FirstName": "I",
"Affiliation": "Nuclear Engineering Program, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil."
},
{
"LastName": "Lopes",
"FirstName": "R T",
"Affiliation": "Nuclear Engineering Program, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil."
}
] | No |
36435122 | The persistent, bioaccumulative, toxic, and resistance (PBTR) risk assessment framework of antibiotics in the drinking water sources. | Antibiotics are emerging pollutants largely considered to have a lower risk based on persistent, bioaccumulative, toxic (PBT) risk assessments. However, an increasing number of studies have illustrated that antibiotics are responsible for the global increase in antimicrobial resistance (AMR), which suggests that the risk of antibiotics has been largely underestimated by using PBT risk assessment. Here, we designed an integrated innovation risk assessment framework of persistent, bioaccumulative, toxic, and resistance (PBTR) that accounts for antibiotic resistance to better represent the antibiotic environmental risk. This novel antibiotic risk assessment framework was further verified via application to 39 target antibiotics in the 23 drinking water sources of the lower Yangtze River (LYR), China, during the normal and flood seasons. In contrast with the PBT assessment, single toxicity assessment and single resistance assessment, in the PBTR assessment, 7 of 39 target antibiotics with bacterial insensitivity were observed to represent a more prominent risk, as were the sites sampled during the flood season with low concentrations but high pollution loads, which confirmed that the sensitivity of PBTR risk assessment was instructive. The PBTR risk assessment for the screened priority antibiotics contributes not only representative data but also an innovative approach for identifying resistance risks. Using the positive matrix factorization (PMF) model, the sources of priority antibiotics can be predicted and thus supported the corresponding policy. Overall, this study first constructed a PBTR risk assessment framework, then applied it to facilitate the accurate management of antibiotic pollution at the basin level. | 2023 Jan 15 | Journal of environmental management | No DOI | [
{
"LastName": "Guo",
"FirstName": "Xinyan",
"Affiliation": "Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Science, Ministry of Ecology and Environment of the People's Republic of China, Nanjing, 210042, China."
},
{
"LastName": "Ni",
"FirstName": "Ni",
"Affiliation": "Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Science, Ministry of Ecology and Environment of the People's Republic of China, Nanjing, 210042, China."
},
{
"LastName": "Shi",
"FirstName": "Mali",
"Affiliation": "Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Science, Ministry of Ecology and Environment of the People's Republic of China, Nanjing, 210042, China."
},
{
"LastName": "Zhang",
"FirstName": "Xiaohui",
"Affiliation": "Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Science, Ministry of Ecology and Environment of the People's Republic of China, Nanjing, 210042, China."
},
{
"LastName": "Yuan",
"FirstName": "Qingbin",
"Affiliation": "State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China."
},
{
"LastName": "Wang",
"FirstName": "Na",
"Affiliation": "Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Science, Ministry of Ecology and Environment of the People's Republic of China, Nanjing, 210042, China. Electronic address: wangna@nies.org."
},
{
"LastName": "Zhang",
"FirstName": "Shenghu",
"Affiliation": "Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Science, Ministry of Ecology and Environment of the People's Republic of China, Nanjing, 210042, China. Electronic address: zsh@nies.org."
},
{
"LastName": "Luo",
"FirstName": "Yi",
"Affiliation": "State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China."
}
] | Yes |
26737409 | A novel ultra-wideband 80 GHz FMCW radar system for contactless monitoring of vital signs. | In this paper an ultra-wideband 80 GHz FMCW-radar system for contactless monitoring of respiration and heart rate is investigated and compared to a standard monitoring system with ECG and CO(2) measurements as reference. The novel FMCW-radar enables the detection of the physiological displacement of the skin surface with submillimeter accuracy. This high accuracy is achieved with a large bandwidth of 10 GHz and the combination of intermediate frequency and phase evaluation. This concept is validated with a radar system simulation and experimental measurements are performed with different radar sensor positions and orientations. | 2015 | Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference | No DOI | [
{
"LastName": "Wang",
"FirstName": "Siying",
"Affiliation": ""
},
{
"LastName": "Pohl",
"FirstName": "Antje",
"Affiliation": ""
},
{
"LastName": "Jaeschke",
"FirstName": "Timo",
"Affiliation": ""
},
{
"LastName": "Czaplik",
"FirstName": "Michael",
"Affiliation": ""
},
{
"LastName": "Köny",
"FirstName": "Marcus",
"Affiliation": ""
},
{
"LastName": "Leonhardt",
"FirstName": "Steffen",
"Affiliation": ""
},
{
"LastName": "Pohl",
"FirstName": "Nils",
"Affiliation": ""
}
] | No |
17635921 | Nicotine-induced activation of AMP-activated protein kinase inhibits fatty acid synthase in 3T3L1 adipocytes: a role for oxidant stress. | Recent studies suggest that the AMP-activated protein kinase (AMPK) acts as a major energy sensor and regulator in adipose tissues. The objective of this study was to investigate the role of AMPK in nicotine-induced lipogenesis and lipolysis in 3T3L1 adipocytes. Exposure of 3T3L1 adipocytes to smoking-related concentrations of nicotine increased lipolysis and inhibited fatty acid synthase (FAS) activity in a time- and dose-dependent manner. The effects of nicotine on FAS activity were accompanied by phosphorylation of both AMPK (Thr(172)) and acetyl-CoA carboxylase (ACC; Ser(79)). Nicotine-induced AMPK phosphorylation appeared to be mediated by reactive oxygen species based on the finding that nicotine significantly increased superoxide anions and 3-nitrotyrosine-positive proteins, exogenous peroxynitrite (ONOO(-)) mimicked the effects of nicotine on AMPK, and N-acetylcysteine (NAC) abolished nicotine-enhanced AMPK phosphorylation. Inhibition of AMPK using either pharmacologic (insulin, compound C) or genetic means (overexpression of dominant negative AMPK; AMPK-DN) abolished FAS inhibition induced by nicotine or ONOO(-). Conversely, activation of AMPK by pharmacologic (nicotine, ONOO(-), metformin, and AICAR) or genetic (overexpression of constitutively active AMPK) means inhibited FAS activity. Notably, AMPK activation increased threonine phosphorylation of FAS, and this effect was blocked by adenovirus encoding dominant negative AMPK. Finally, AMPK-dependent FAS phosphorylation was confirmed by (32)P incorporation into FAS in adipocytes. Taken together, our results strongly suggest that nicotine, via ONOO(-) activates AMPK, resulting in enhanced threonine phosphorylation and consequent inhibition of FAS. | 2007 Sep 14 | The Journal of biological chemistry | No DOI | [
{
"LastName": "An",
"FirstName": "Zhibo",
"Affiliation": "Vascular Biology Laboratory, Department of Surgery, Graduate School of Medicine, University of Tennessee, Knoxville, Tennessee 37922."
},
{
"LastName": "Wang",
"FirstName": "Hong",
"Affiliation": "Division of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73104."
},
{
"LastName": "Song",
"FirstName": "Ping",
"Affiliation": "Division of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73104."
},
{
"LastName": "Zhang",
"FirstName": "Miao",
"Affiliation": "Division of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73104."
},
{
"LastName": "Geng",
"FirstName": "Xuemei",
"Affiliation": "Vascular Biology Laboratory, Department of Surgery, Graduate School of Medicine, University of Tennessee, Knoxville, Tennessee 37922."
},
{
"LastName": "Zou",
"FirstName": "Ming-Hui",
"Affiliation": "Vascular Biology Laboratory, Department of Surgery, Graduate School of Medicine, University of Tennessee, Knoxville, Tennessee 37922; Division of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73104. Electronic address: ming-hui-zou@ouhsc.edu."
}
] | Yes |
24423289 | Clinical experience in the screening and management of a large kindred with familial isolated pituitary adenoma due to an aryl hydrocarbon receptor interacting protein (AIP) mutation. | Germline AIP mutations usually cause young-onset acromegaly with low penetrance in a subset of familial isolated pituitary adenoma families. We describe our experience with a large family with R304* AIP mutation and discuss some of the diagnostic dilemmas and management issues. The aim of the study was to identify and screen mutation carriers in the family. Forty-three family members participated in the study. The study was performed in university hospitals. We conducted genetic and endocrine screening of family members. We identified 18 carriers of the R304* mutation, three family members with an AIP-variant A299V, and two family members who harbored both changes. One of the two index cases presented with gigantism and pituitary apoplexy, the other presented with young-onset acromegaly, and both had surgery and radiotherapy. After genetic and clinical screening of the family, two R304* carriers were diagnosed with acromegaly. They underwent transsphenoidal surgery after a short period of somatostatin analog treatment. One of these two patients is in remission; the other achieved successful pregnancy despite suboptimal control of acromegaly. One of the A299V carrier family members was previously diagnosed with a microprolactinoma; we consider this case to be a phenocopy. Height of the unaffected R304* carrier family members is not different compared to noncarrier relatives. Families with AIP mutations present particular problems such as the occurrence of large invasive tumors, poor response to medical treatment, difficulties with fertility and management of pregnancy, and the finding of AIP sequence variants of unknown significance. Because disease mostly develops at a younger age and penetrance is low, the timing and duration of the follow-up of carriers without overt disease requires further study. The psychological and financial impact of prolonged clinical screening must be considered. Excellent relationships between the family, endocrinologists, and geneticists are essential, and ideally these families should be managed in centers with specialist expertise. | 2014 Apr | The Journal of clinical endocrinology and metabolism | No DOI | [
{
"LastName": "Williams",
"FirstName": "Fred",
"Affiliation": "Regional Center for Endocrinology and Diabetes (F.W., S.H., A.B.A.), Royal Victoria Hospital, Belfast BT12 6BA, Northern Ireland, United Kingdom; Department of Medical Genetics (L.B., P.J.M.), Belfast Health and Social Care Trust, Belfast BT9 7AB, Northern Ireland, United Kingdom; Department of Endocrinology (H.S.C., H.L.S., S.A.A., M.K.), Barts and London School of Medicine, Queen Mary University of London, London EC1A 6BQ, United Kingdom; North East Thames Regional Genetics Service (A.V.K.), Great Ormond Street Hospital, London WC1N 3JH, United Kingdom; Department of Endocrinology (S.M.O.), St James University Hospital, Leeds LS9 7TF, United Kingdom; Department of Radiology (J.E.), St Bartholomew Hospital, London EC1A 7BE, United Kingdom; and Department of Endocrinology (N.A.), Royal Belfast Hospital for Sick Children, Belfast, BT12 6BA, United Kingdom."
},
{
"LastName": "Hunter",
"FirstName": "Steven",
"Affiliation": ""
},
{
"LastName": "Bradley",
"FirstName": "Lisa",
"Affiliation": ""
},
{
"LastName": "Chahal",
"FirstName": "Harvinder S",
"Affiliation": ""
},
{
"LastName": "Storr",
"FirstName": "Helen L",
"Affiliation": ""
},
{
"LastName": "Akker",
"FirstName": "Scott A",
"Affiliation": ""
},
{
"LastName": "Kumar",
"FirstName": "Ajith V",
"Affiliation": ""
},
{
"LastName": "Orme",
"FirstName": "Stephen M",
"Affiliation": ""
},
{
"LastName": "Evanson",
"FirstName": "Jane",
"Affiliation": ""
},
{
"LastName": "Abid",
"FirstName": "Noina",
"Affiliation": ""
},
{
"LastName": "Morrison",
"FirstName": "Patrick J",
"Affiliation": ""
},
{
"LastName": "Korbonits",
"FirstName": "Márta",
"Affiliation": ""
},
{
"LastName": "Atkinson",
"FirstName": "A Brew",
"Affiliation": ""
}
] | No |
35756422 | Excimer Laser Corneal Refractive Surgery in the Clinic: A Systematic Review and Meta-analysis. | To systematically evaluate the efficacy, safety, recovery speed, and long-term visual quality of excimer laser corneal refractive surgery and to provide evidence-based medicine for the promotion and use of excimer laser corneal refractive surgery. Randomized controls on excimer laser refractive surgery in Web of science, PubMed, EMBASE, ScienceDirect, Cochrane Library, China Knowledge Network (CNKI), China VIP Database, Wan Fang Database, and China Biomedical Literature Database (CBM) were searched by the computer. Randomized controlled trial (RCT) data were extracted independently by two researchers, and the risk of bias of each included RCT was assessed according to the Cochrane Handbook 5.1.0 criteria. Meta-analysis of the collected data was performed using RevMan5.4 statistical software. In the end, 9 high-quality literatures were included, with a total of 4366 samples, and meta-analysis was used. There was no significant difference in uncorrected visual acuity WMD after excimer laser keratorefractive surgery, but there was a statistically significant difference in WMD in the safety of excimer laser keratorefractive surgery. The results of uncorrected visual acuity (close) indicated the following: Chi[2] = 13.56, DF = 5, P = 0.02, and I[2] = 100%; the results of uncorrected visual acuity (distance) indicated the following: Chi[2] = 34.44, DF =5 (P < 00000), and I[2] = 85%; the results of best corrected visual acuity (myopia) indicated the following: Chi[2] = 0.65, DF = 3, P = 088 > 0.05, and I[2] = 0%; the results of best corrected visual acuity (hyperopia) indicated the following: Chi[2] = 1.80, DF = 3, P = 0.61 > 0.05, and I[2] = 0%. Excimer laser corneal refractive surgery is safe and effective, with faster recovery and better long-term visual acuity treatment effect. However, more studies and follow-up with higher methodological quality and longer intervention time are needed for further validation. | 2022 | Computational and mathematical methods in medicine | No DOI | [
{
"LastName": "Zhang",
"FirstName": "Huang",
"Affiliation": "Department of Ophtalmology, Zhongshan Torch Development Zone People's Hospital, Zhongshan 528437, Guangdong Province, China."
},
{
"LastName": "Li",
"FirstName": "Mingming",
"Affiliation": "Department of Ophtalmology, Zhongshan Torch Development Zone People's Hospital, Zhongshan 528437, Guangdong Province, China."
},
{
"LastName": "Cen",
"FirstName": "Zhimin",
"Affiliation": "Department of Ophtalmology, Zhongshan Torch Development Zone People's Hospital, Zhongshan 528437, Guangdong Province, China."
}
] | Yes |
38983991 | Isolated Volar Radioulnar Joint Dislocation With Associated Ulnar Styloid Fracture. | Isolated volar dislocation of the distal radioulnar joint is a rare occurrence and is commonly missed. The mechanism of injury typically involves hypersupination. True lateral radiographs are difficult to obtain as patients are usually limited with wrist pronation and supination, resulting in a high miss rate. We describe a 32-year-old male who presented to the emergency department (ED) with pain and swelling of the posteromedial aspect of the right wrist after punching a wall one hour prior to presentation. Examination revealed soft tissue tenderness and mild edema at the right distal ulna with an associated deformity, best visualized at the volar aspect of the right wrist. Active range of motion was limited with right wrist flexion and extension, secondary to pain and edema. Right wrist supination and pronation strength and range of motion were limited due to the patient's tenderness on examination. Peripheral nerve function and vascular examination were normal. Initial radiographs of the right hand, wrist, and forearm did not reveal a fracture or dislocation. A musculoskeletal computed tomography (CT) scan of the right hand and wrist revealed an avulsion fracture of the ulnar styloid with volar displacement of the ulna. Analgesia was achieved with an ultrasound-guided ulnar nerve block, and the right wrist was successfully reduced. This report highlights the difficulty in obtaining a diagnosis of an isolated volar dislocation of the distal radioulnar joint. We recommend obtaining a musculoskeletal CT scan in the setting of an inconclusive radiograph and incongruent physical examination. Analgesia can also be achieved with an ulnar nerve block under ultrasound guidance. | 2024 Jun | Cureus | No DOI | [
{
"LastName": "Israelyan",
"FirstName": "Arman",
"Affiliation": "Emergency Department, Kaiser Permanente Modesto Medical Center, Modesto, USA."
},
{
"LastName": "Chiang",
"FirstName": "James",
"Affiliation": "Emergency Department, Kaiser Permanente Modesto Medical Center, Modesto, USA."
},
{
"LastName": "Cooper",
"FirstName": "Kassandra C",
"Affiliation": "Emergency Department, Kaiser Permanente Modesto Medical Center, Modesto, USA."
},
{
"LastName": "Lew",
"FirstName": "Valerie L",
"Affiliation": "Emergency Department, Kaiser Permanente Modesto Medical Center, Modesto, USA."
},
{
"LastName": "Murphey",
"FirstName": "Gary C",
"Affiliation": "Emergency Department, Kaiser Permanente Modesto Medical Center, Modesto, USA."
},
{
"LastName": "Durant",
"FirstName": "Edward",
"Affiliation": "Emergency Department, Kaiser Permanente Modesto Medical Center, Modesto, USA."
}
] | No |
33198521 | Impact of perceived contraceptive policy changes on long acting reversible contraception dispensing trends. | We observed the long-term trend of Long Acting Reversible Contraception (LARC) usage before and after the 2016 presidential election. We observed the rate of LARC dispensed at a university student health centre in the 18 months preceding and 27 months following the 2016 U.S. presidential election which posed threat to contraception access. We applied a segmented regression model using two linear regression line segments to evaluate whether there is a time point where the trend of LARC dispensing changed. We fit the regression models with a breakpoint at month 0 (election month) and 3 months with a Locally Estimated Scatterplot Smoothing (LOESS) estimate with parameters obtained by estimating simple linear regression models separately below and above the breakpoint '0'. There were a total of 2067 LARC methods dispensed from May 2015 to February 2019. The average number of LARC methods dispensed before November 2016 was 38/month and increased to 51/month post-presidential election. The LARC dispense rate significantly increased each month (0.38, 1.74; 95% confidence level, p < 0.05) until a breakpoint at 6 months (standard error 4.11) post-election followed by slower decrease (-0.59/month, 95% confidence level: -1.37, 0.20; p=not significant). Our study is the first to report long-term trends (27 months post-election) in LARC uptake amidst the public discourse that suggested the end of a policy that provided LARC insurance coverage. Although this observational study cannot suggest causality, the findings could reflect actions taken to prevent unintended pregnancy in response to the event of uncertain national policy. | 2021 Feb | The European journal of contraception & reproductive health care : the official journal of the European Society of Contraception | No DOI | [
{
"LastName": "Sridhar",
"FirstName": "Aparna",
"Affiliation": "Department of Obstetrics and Gynecology, University of California Los Angeles, Los Angeles, CA, USA."
},
{
"LastName": "Friedman",
"FirstName": "Sarah R",
"Affiliation": "Department of Urology, University of California Los Angeles, Los Angeles, CA, USA."
},
{
"LastName": "Sim",
"FirstName": "Myung-Shin K",
"Affiliation": "University of California Los Angeles General Internal Medicine & Health Services Research, Los Angeles, CA, USA."
},
{
"LastName": "Troung",
"FirstName": "William",
"Affiliation": "University of California Los Angeles Arthur Ashe Student Health & Wellness Center, Los Angeles, CA, USA."
},
{
"LastName": "Elias",
"FirstName": "Sam",
"Affiliation": "University of California Los Angeles Arthur Ashe Student Health & Wellness Center, Los Angeles, CA, USA."
}
] | No |
29536729 | Precision-Guided Nanospears for Targeted and High-Throughput Intracellular Gene Delivery. | An efficient nonviral platform for high-throughput and subcellular precision targeted intracellular delivery of nucleic acids in cell culture based on magnetic nanospears is reported. These magnetic nanospears are made of Au/Ni/Si (∼5 μm in length with tip diameters <50 nm) and fabricated by nanosphere lithography and metal deposition. A magnet is used to direct the mechanical motion of a single nanospear, enabling precise control of position and three-dimensional rotation. These nanospears were further functionalized with enhanced green fluorescent protein (eGFP)-expression plasmids via a layer-by-layer approach before release from the underlying silicon substrate. Plasmid functionalized nanospears are guided magnetically to approach target adherent U87 glioblastoma cells, penetrating the cell membrane to enable intracellular delivery of the plasmid cargo. After 24 h, the target cell expresses green fluorescence indicating successful transfection. This nanospear-mediated transfection is readily scalable for the simultaneous manipulation of multiple cells using a rotating magnet. Cell viability >90% and transfection rates >80% were achieved, which exceed conventional nonviral intracellular methods. This approach is compatible with good manufacturing practices, circumventing barriers to the translation and clinical deployment of emerging cellular therapies. | 2018 May 22 | ACS nano | No DOI | [
{
"LastName": "Xu",
"FirstName": "Xiaobin",
"Affiliation": "California NanoSystems Institute , University of California, Los Angeles , Los Angeles , California 90095 , United States."
},
{
"LastName": "Hou",
"FirstName": "Shuang",
"Affiliation": "California NanoSystems Institute , University of California, Los Angeles , Los Angeles , California 90095 , United States."
},
{
"LastName": "Wattanatorn",
"FirstName": "Natcha",
"Affiliation": "California NanoSystems Institute , University of California, Los Angeles , Los Angeles , California 90095 , United States."
},
{
"LastName": "Wang",
"FirstName": "Fang",
"Affiliation": "California NanoSystems Institute , University of California, Los Angeles , Los Angeles , California 90095 , United States."
},
{
"LastName": "Yang",
"FirstName": "Qing",
"Affiliation": "California NanoSystems Institute , University of California, Los Angeles , Los Angeles , California 90095 , United States."
},
{
"LastName": "Zhao",
"FirstName": "Chuanzhen",
"Affiliation": "California NanoSystems Institute , University of California, Los Angeles , Los Angeles , California 90095 , United States."
},
{
"LastName": "Yu",
"FirstName": "Xiao",
"Affiliation": "California NanoSystems Institute , University of California, Los Angeles , Los Angeles , California 90095 , United States."
},
{
"LastName": "Tseng",
"FirstName": "Hsian-Rong",
"Affiliation": "California NanoSystems Institute , University of California, Los Angeles , Los Angeles , California 90095 , United States."
},
{
"LastName": "Jonas",
"FirstName": "Steven J",
"Affiliation": "California NanoSystems Institute , University of California, Los Angeles , Los Angeles , California 90095 , United States."
},
{
"LastName": "Weiss",
"FirstName": "Paul S",
"Affiliation": "California NanoSystems Institute , University of California, Los Angeles , Los Angeles , California 90095 , United States."
}
] | Yes |
36277037 | The Effectiveness and Safety of Mesenchymal Stem Cells in the Treatment of Osteoarthritis: A Systematic Review and Meta-analysis of 28 Randomized Controlled Trials. | To evaluate the effectiveness and safety of mesenchymal stem cells (MSCs) in the treatment of osteoarthritis (OA). Chinese databases (such as CNKI and SinoMed) and English databases (such as PubMed and Embase) were searched to collect randomized controlled trials (RCTs) of MSCs in the treatment of OA. The retrieval time is from inception to October 10, 2021. The literature was strictly selected according to the inclusion and exclusion criteria, data was extracted, and the quality was evaluated. RevMan 5.3 software was used for meta-analysis. STATA was used to evaluate publication bias. The registration number of this systematic review and meta-analysis is CRD42021277145. A total of 28 RCTs involving 1494 participants were included. The primary outcomes showed that MSCs may reduce WOMAC pain and VAS at the 3rd-month follow-up [WOMAC pain: -3.81 (-6.95, -0.68), P = 0.02. VAS: -1.11 (-1.53, -0.68), P < 0.00001], and the effect lasts for at least 12 months [WOMAC pain: -4.29 (-7.12, -1.47), P = 0.003. VAS: -1.77 (-2.43, -1.12), P < 0.00001]. MSCs may also reduce WOMAC stiffness and physical function at the 6th-month follow-up [WOMAC stiffness: -1.12 (-2.09, -0.14), P = 0.03. WOMAC physical function: -4.40 (-6.84, -1.96), P = 0.0004], and the effect lasts for at least 12 months [WOMAC stiffness: -0.99 (-1.95, -0.03), P = 0.04. WOMAC physical function: -3.26 (-5.91, -0.61), P = 0.02]. The improvement of WOMAC pain, VAS, WOMAC stiffness, and WOMAC physical function may be clinically significant. Meanwhile, after the MSC injection, Lequesne had been reduced compared with the control group [-4.49 (-8.21, -0.77), P = 0.002]. For adverse events, there is no significant difference in the safety of MSC injection and the control group [1.20 (0.97, 1.48), P = 0.09]. The quality of WOMAC physical function and adverse events were moderate. Based on current evidence, MSCs may be a safety therapy that have a good curative effect in the treatment of OA, the onset time is no later than 3 months, and the time to maintain the curative effect is no less than 12 months. However, these results should be generalized with caution due to the generally low quality of evidence and RCTs. | 2022 | Stem cells international | No DOI | [
{
"LastName": "Long",
"FirstName": "Zhiyong",
"Affiliation": "Department of Rehabilitation Medicine, Guangzhou Panyu Central Hospital, Guangzhou, China."
},
{
"LastName": "Zhang",
"FirstName": "Mingsheng",
"Affiliation": "Department of Rehabilitation Medicine, South China Hospital of Shenzhen University, Shenzhen, China."
},
{
"LastName": "Zhang",
"FirstName": "Tianqing",
"Affiliation": "Hunan University of Chinese Medicine, Changsha, Hunan, China."
},
{
"LastName": "Zeng",
"FirstName": "Liuting",
"Affiliation": "Hunan University of Chinese Medicine, Changsha, Hunan, China."
},
{
"LastName": "Yang",
"FirstName": "Kailin",
"Affiliation": "Hunan University of Chinese Medicine, Changsha, Hunan, China."
},
{
"LastName": "Yang",
"FirstName": "Tiejun",
"Affiliation": "Hunan University of Chinese Medicine, Changsha, Hunan, China."
},
{
"LastName": "Yu",
"FirstName": "Ganpeng",
"Affiliation": "Hunan University of Chinese Medicine, Changsha, Hunan, China."
},
{
"LastName": "Li",
"FirstName": "Jun",
"Affiliation": "Hunan University of Chinese Medicine, Changsha, Hunan, China."
},
{
"LastName": "Wu",
"FirstName": "Yang",
"Affiliation": "Hunan University of Chinese Medicine, Changsha, Hunan, China."
},
{
"LastName": "Chen",
"FirstName": "Hua",
"Affiliation": "Hunan University of Chinese Medicine, Changsha, Hunan, China."
}
] | No |
17282114 | On-line estimation of propofol pharmacodynamic parameters. | A novel advisory system, the Anesthesia Advisory Display (AAD) has been recently developed. It displays information about actual and future predictions of anesthetic drug concentrations mapped to clinical end-points such as BIS, basing on the actual infusion rate combined with population pharmacokinetic and interaction pharmacodynamic (PKPD) models. According to the large variability observed among patients in clinical end-points to the same dosing history, a population model could fail in predicting the real patient's behavior: an "individualized" model is then necessary. An on-line estimation of the model's parameter for the single patients has been developed, basing on the extended Kalman filter algorithm. The method has been tested on data of 40 patients from a previous clinical study and the prediction of the individual models have been compared with the population model's. | 2005 | Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference | No DOI | [
{
"LastName": "Sartori",
"FirstName": "Valentina",
"Affiliation": "Automatic Control Laboratory, Swiss Federal Institute of Technology (ETH), CH - 8092 Zurich, Switzerland, sartori@control.ee.ethz.ch."
},
{
"LastName": "Schumacher",
"FirstName": "Peter",
"Affiliation": ""
},
{
"LastName": "Bouillon",
"FirstName": "Thomas",
"Affiliation": ""
},
{
"LastName": "Luginbuehl",
"FirstName": "Martin",
"Affiliation": ""
},
{
"LastName": "Morari",
"FirstName": "Manfred",
"Affiliation": ""
}
] | No |
28462251 | Can Improving Knowledge of Antibiotic-Associated Adverse Drug Events Reduce Parent and Patient Demand for Antibiotics? | According to the Centers for Disease Control and Prevention, at least 2 million people are infected and 23,000 die each year in the United States as a result of antibiotic-resistant bacterial infections. Antibiotic use is the most important factor contributing to antibiotic resistance and overuse is common, especially for upper respiratory tract infections. There is a perception among the public, as well as some health care providers, that antibiotics are harmless. We conducted formative research to explore patient and parent knowledge and attitudes relating to antibiotic use and adverse drug events (ADEs). Six computer-assisted telephone focus groups were conducted in October and November 2010 with adult patients and mothers of young children. The focus groups were developed to engage participants in discussion about their knowledge and attitudes regarding antibiotic resistance and ADEs associated with antibiotic use. Nearly all mothers were familiar with the possibility of "side effects" with prescription medications, including antibiotics. However, very few mothers were familiar with severe antibiotic-associated ADEs and nearly all felt strongly that this information should be shared with parents at the time a prescription is recommended or written for their child. Adult participants did not believe that the potential for ADEs was a significant issue for adults and most reported never discussing the potential for adverse events with their provider. Parents were receptive to appropriate antibiotic use messaging around ADEs. We learned that ADE messages did not resonate with adults in the same way they did with mothers of young children. | 2015 Jan-Dec | Health services research and managerial epidemiology | No DOI | [
{
"LastName": "Roberts",
"FirstName": "Rebecca M",
"Affiliation": "Get Smart: Know When Antibiotics Work program, Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA."
},
{
"LastName": "Albert",
"FirstName": "Alison P",
"Affiliation": "Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA."
},
{
"LastName": "Johnson",
"FirstName": "Darcia D",
"Affiliation": "Get Smart: Know When Antibiotics Work program, Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA."
},
{
"LastName": "Hicks",
"FirstName": "Lauri A",
"Affiliation": "Get Smart: Know When Antibiotics Work program, Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA."
}
] | No |
29312489 | Melatonin represses oral squamous cell carcinoma metastasis by inhibiting tumor-associated neutrophils. | Tumor-associated neutrophils (TANs) promote metastasis of multiple cancers, including oral squamous cell carcinoma (OSCC). Melatonin (Mel) reportedly exerts anti-metastatic effects on OSCC. However, little is known about the anti-OSCC effects of Mel involved in TANs. In this study, intensive infiltration of TANs was positively associated with advanced stage, lymphatic metastasis, and poor prognosis of OSCC. Moreover, Mel reduced the survival and migration of OSCC-associated neutrophils. Mechanistically, Mel suppressed the TAN release of C-X-C motif chemokine ligand 8, C-C motif chemokine ligand 2 (CCL2), CCL4, and matrix metalloproteinase-9 by blockage of p38 MAPK and Akt signaling. Mel-fostered TANs decreased the migration and invasion of OSCC cells and reduced tube formation in vitro. Additionally, Mel-hampered pro-motility and pro-angiogenesis effects of TANs were dependent on MMP-9 suppression in OSCC. Overall, The beneficial roles of melatonin in retarding OSCC metastasis were implicated with inhibition of TANs. | 2017 | American journal of translational research | No DOI | [
{
"LastName": "Lu",
"FirstName": "Haibin",
"Affiliation": "Department of Oral and Maxillofacial Surgery, Zhongshan Hospital Affiliated to Dalian UniversityDalian, 116001 Liaoning, China."
},
{
"LastName": "Wu",
"FirstName": "Baolei",
"Affiliation": "Department of Oral and Maxillofacial Surgery, No.3 Hospital of People's Liberation ArmyBaoji 721004, Shannxi, China."
},
{
"LastName": "Ma",
"FirstName": "Ge",
"Affiliation": "Department of Oral and Maxillofacial Surgery, No.3 Hospital of People's Liberation ArmyBaoji 721004, Shannxi, China."
},
{
"LastName": "Zheng",
"FirstName": "Deyu",
"Affiliation": "Department of Oral and Maxillofacial Surgery, No.3 Hospital of People's Liberation ArmyBaoji 721004, Shannxi, China."
},
{
"LastName": "Song",
"FirstName": "Ruijuan",
"Affiliation": "Department of Oral and Maxillofacial Surgery, No.3 Hospital of People's Liberation ArmyBaoji 721004, Shannxi, China."
},
{
"LastName": "Huang",
"FirstName": "Erjiang",
"Affiliation": "Department of Oral and Maxillofacial Surgery, No.3 Hospital of People's Liberation ArmyBaoji 721004, Shannxi, China."
},
{
"LastName": "Mao",
"FirstName": "Ming",
"Affiliation": "Department of Oral and Maxillofacial Surgery, No.3 Hospital of People's Liberation ArmyBaoji 721004, Shannxi, China."
},
{
"LastName": "Lu",
"FirstName": "Bin",
"Affiliation": "State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical UniversityXi'an 710032, Shannxi, China."
}
] | No |
36017389 | Deconstruction of the Prevention of Knee Osteoarthritis by Swimming Based on Data Mining Technology. | With the continuous development of big data and the continuous improvement of people's living standards, increasingly attention is paid to physical health. Swimming in this sport is effective in preventing the occurrence of arthritis. This paper analyzes the prevention and exploration of arthritis and relies on the traditional method of retrieving clinical literature on the treatment of knee osteoarthritis with traditional Chinese medicine and internal medicine, which requires a lot of manpower and material resources. At this time, the role of data mining technology is brought into play. This article analyzes the prevention of arthritis by swimming. If you rely on the traditional retrieval of clinical literature on the treatment of knee osteoarthritis with traditional Chinese medicine and internal medicine, you will find a lot of disordered data. It takes a lot of manpower and material resources to sort out the summary, and at this time, the role of data mining (DM) technology is brought into play. In this paper, the relevant information of the literature that meets the requirements is established in an Excel database, and the data of the relevant information is entered. Through sorting and analysis, the TCM syndrome types of knee osteoarthritis are summarized. Then, DM technology was used to carry out statistical analysis of frequency and prescription, to summarize the distribution characteristics of the corresponding knee osteoarthritis, TCM syndrome types, and the weight of each syndrome type, and to make a preliminary discussion at the same time. Finally, it is concluded that there are better prevention methods for arthritis in the research methods of traditional Chinese medicine. DM technology has been increasingly applied to all aspects of traditional Chinese medicine. DM technology has improved its research efficiency by 38% and achieved great results, which will play a greater role in promoting the research process of TCM syndrome. | 2022 | BioMed research international | No DOI | [
{
"LastName": "Yin",
"FirstName": "Jianxia",
"Affiliation": "Swimming Teaching and Research Office, Xi'an Physical Education University, Xi'an, 710068 Shaanxi, China."
},
{
"LastName": "Li",
"FirstName": "Qing",
"Affiliation": "Swimming Teaching and Research Office, Xi'an Physical Education University, Xi'an, 710068 Shaanxi, China."
},
{
"LastName": "Song",
"FirstName": "Yao",
"Affiliation": "Competition Training Division, Xi'an Physical Education University, Xi'an, 710068 Shaanxi, China."
}
] | Yes |
31344658 | lncRNA MALAT1 Accelerates Wound Healing of Diabetic Mice Transfused with Modified Autologous Blood via the HIF-1α Signaling Pathway. | Impaired wound healing is a debilitating complication of diabetes. The long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been recognized to be differentially expressed in various diseases. However, its underlying mechanism in diabetes has not been fully understood. Notably, we aim to examine the expression of MALAT1 in diabetic mice and its role in wound healing involving the hypoxia-inducible factor-1α (HIF-1α) signaling pathway with a modified autologous blood preservative solution reported. A mouse model of diabetes was established. MALAT1 was identified to promote the activation of the HIF-1α signaling pathway and to be enriched in autologous blood through modified preservation, which might facilitate the improvement of physiological function of blood cells. Through gain- or loss-of-function approaches, viability of fibroblasts cultured in high glucose, wound healing of mice, and collagen expression in wound areas were enhanced by MALAT1 and HIF-1α. Taken together, the present study demonstrated that the physiological status of mouse blood was effectively improved by modified autologous blood preservation, which exhibited upregulated MALAT1, thereby accelerating the fibroblast activation and wound healing in diabetic mice via the activation of the HIF-1α signaling pathway. The upregulation of MALAT1 activating the HIF-1α signaling pathway provides a novel insight into drug targets against diabetes. | 2019 Sep 6 | Molecular therapy. Nucleic acids | No DOI | [
{
"LastName": "Liu",
"FirstName": "Xiao-Qian",
"Affiliation": "Department of Anesthesiology, Gongli Hospital, The Second Military Medical University, Shanghai 200135, P.R. China."
},
{
"LastName": "Duan",
"FirstName": "Li-Shuang",
"Affiliation": "Department of Anesthesiology, Yijishan Hospital, Wannan Medical College, Wuhu 241000, P.R. China."
},
{
"LastName": "Chen",
"FirstName": "Yong-Quan",
"Affiliation": "Department of Anesthesiology, Yijishan Hospital, Wannan Medical College, Wuhu 241000, P.R. China."
},
{
"LastName": "Jin",
"FirstName": "Xiao-Ju",
"Affiliation": "Department of Anesthesiology, Yijishan Hospital, Wannan Medical College, Wuhu 241000, P.R. China."
},
{
"LastName": "Zhu",
"FirstName": "Na-Na",
"Affiliation": "Department of Anesthesiology, Gongli Hospital, The Second Military Medical University, Shanghai 200135, P.R. China."
},
{
"LastName": "Zhou",
"FirstName": "Xun",
"Affiliation": "Department of Anesthesiology, Gongli Hospital, The Second Military Medical University, Shanghai 200135, P.R. China."
},
{
"LastName": "Wei",
"FirstName": "Han-Wei",
"Affiliation": "Department of Anesthesiology, Gongli Hospital, The Second Military Medical University, Shanghai 200135, P.R. China."
},
{
"LastName": "Yin",
"FirstName": "Lei",
"Affiliation": "Department of Anesthesiology, Gongli Hospital, The Second Military Medical University, Shanghai 200135, P.R. China."
},
{
"LastName": "Guo",
"FirstName": "Jian-Rong",
"Affiliation": "Department of Anesthesiology, Gongli Hospital, The Second Military Medical University, Shanghai 200135, P.R. China. Electronic address: gjrgjr929@163.com."
}
] | Yes |
29326613 | French Translation and Validation of Three Scales Evaluating Stigma in Mental Health. | The concept of stigma refers to problems of knowledge (ignorance), attitudes (prejudice), and behavior (discrimination). Stigma may hinder access to care, housing, and work. In the context of implementation of programs such as "housing first" or "individual placement and support" in French speaking regions, validated instruments measuring stigma are necessary. "Attitudes to Mental Illness 2011" is a questionnaire that includes three scales measuring stigma through these three dimensions. This study aimed to translate, adapt, and validate these three scales in French. The "Attitudes to Mental Illness 2011" questionnaire was translated into French and back-translated into English by an expert. Two hundred and sixty-eight nursing students completed the questionnaire. Content validity, face validity, internal validity, and convergent validity were assessed. Long-term reliability was also estimated over a three-month period. Experts and participants found that the questionnaire's content validity and face validity were appropriate. The internal validities of the three scales were also considered adequate. Convergent validity indicated that the scales did indeed measure what they were supposed to. Long-term stability estimates were moderate; this pattern of results suggested that the construct targeted by the three scales is adequately measured but does not necessarily represent stable and enduring traits. Because of their good psychometric properties, these three scales can be used in French, either separately, to measure one specific dimension of stigma, or together, to assess stigma in its three dimensions. This would seem of paramount importance in evaluating campaigns against stigma since it allows measures to be adapted according to campaign goals and the target population. | 2017 | Frontiers in psychiatry | No DOI | [
{
"LastName": "Garcia",
"FirstName": "Carla",
"Affiliation": "Community Psychiatry Unit, Department of Psychiatry, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland."
},
{
"LastName": "Golay",
"FirstName": "Philippe",
"Affiliation": "Community Psychiatry Unit, Department of Psychiatry, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland."
},
{
"LastName": "Favrod",
"FirstName": "Jérôme",
"Affiliation": "Community Psychiatry Unit, Department of Psychiatry, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland."
},
{
"LastName": "Bonsack",
"FirstName": "Charles",
"Affiliation": "Community Psychiatry Unit, Department of Psychiatry, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland."
}
] | No |
37780543 | Prognosis of limb‑salvage treatment of osteosarcoma in adolescent patients: a meta‑analysis. | To evaluate the effectiveness of limb-salvage treatment for osteosarcoma in adolescent patients, a comprehensive search on PubMed, Embase and Cochrane Library was conducted. Studies with a clear diagnosis of osteosarcoma were included and duplicate publications, studies without full text or incomplete information, those with an inability to extract data, divergent definitions of exposure, animal experiments, reviews, and systematic reviews were excluded. The data were analyzed using STATA 15.1. The findings of the present study revealed that overall survival (OS) and progression-free survival (PFS) of patients with osteosarcoma in the limb-salvage treatment group were significantly longer than those in the amputation treatment group [hazard ratio (HR)=0.71; 95% confidence interval (CI): 0.63-0.80; P=0.000 vs. HR=0.60; 95% CI: 0.48-0.76; P=0.000]. Additionally, the five-year OS rate for patients in the limb-salvage treatment group was higher than that in the amputation group [odds ratio (OR)=4.48; 95% CI: 2.74-7.31; P=0.000]. However, the local recurrence rate was notably higher in the limb-salvage treatment group compared with the amputation treatment group (OR=2.68; 95% CI: 1.50-4.77; P=0.001). Furthermore, the results indicated no significant difference in distant metastasis rates between the limb-salvage treatment group and the amputation treatment group (OR=0.32; 95% CI: 0.10-1.06; P=0.062). In conclusion, the present meta-analysis underscores the potential of limb-salvage therapy for adolescent patients with osteosarcoma. The OS and PFS of patients undergoing limb-salvage surgery are longer than those of amputation, with a higher five-year OS rate and a similar rate of distant metastasis. However, the local recurrence rate of limb-salvage surgery is significantly higher than that of amputation. | 2023 Nov | Oncology letters | No DOI | [
{
"LastName": "Wang",
"FirstName": "Lirong",
"Affiliation": "Department of Nursing, The People's Hospital of YueChi County, Guang'an, Sichuan 638373, P.R. China."
},
{
"LastName": "Fan",
"FirstName": "Yong",
"Affiliation": "Department of Orthopedic Surgery, The People's Hospital of RenShou County, Meishan, Sichuan 620500, P.R. China."
},
{
"LastName": "Zhou",
"FirstName": "Yu",
"Affiliation": "Department of Orthopedic Surgery, The People's Hospital of RenShou County, Meishan, Sichuan 620500, P.R. China."
},
{
"LastName": "Zhong",
"FirstName": "Gang",
"Affiliation": "Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China."
}
] | No |
39035091 | Variable embodiment of stance-taking and footing in simultaneous interpreting. | Previous research has argued that consecutive interpreters constitute laminated speakers in the sense that they engage with different kinds of footing at once, representing another's point of view through their words in another language. These multiple roles also play out in their gesturing, as they sometimes indicate deictically who is the source of the ideas and stances they are expressing (the principal). Simultaneous interpreters, though, often work in an interpreting booth; they are often not seen by the audience, yet many of them gesture, sometimes frequently. How are simultaneous interpreters using gesture in relation to stance-taking and footing? We consider the case of simultaneous interpreters rendering popular science lectures between (both to and from) Russian (their L1) and either English or German (their L2). Though only hearing the audio of the lectures, the interpreters produced many gestures, which were analyzed for their function. Some representational and deictic gestures appeared to clearly involve the interpreter as the principal (writing numbers with one's finger to help remember them or pointing to two places on the desk to keep track of two different quantities mentioned). Other representational and deictic gestures are ambiguous as to whether they are enacting what the interpreter may have imagined what the lecturer did or whether they arose out of the interpreter's own thinking for speaking (e.g., tracing the form of a bird being mentioned or pointing to an empty space when the lecturer was referring to a graph). Pragmatic gestures, showing one's stance toward the topic of the talk, were the most ambiguous as to the footing, reflecting how the interpreter may be engaged in fictive interaction with their imagined audience. Self-adapters, however, more clearly involve the interpreter as the principal, as such actions are known to support cognitive focussing and self-soothing. In sum, we see varying degrees of clarity as to whose stance and principal footing simultaneous interpreters are expressing bodily as laminated speakers. The variable ambiguity can be attributed to the nature of gesture as a semiotic system, the functions of which are more often dependent on co-occurring speech than vice versa. | 2024 | Frontiers in psychology | No DOI | [
{
"LastName": "Cienki",
"FirstName": "Alan",
"Affiliation": "Department of Language, Literature, and Communication, Faculty of Humanities, Vrije Universiteit Amsterdam, Amsterdam, Netherlands."
}
] | No |
29675576 | Gintonin Mitigates MPTP-Induced Loss of Nigrostriatal Dopaminergic Neurons and Accumulation of α-Synuclein via the Nrf2/HO-1 Pathway. | Gintonin, a ginseng-derived glycolipoprotein isolated from ginseng, has been shown to be neuroprotective in several neurological disorders such as Alzheimer's disease models and depressive-like behaviors. In this study, we sought to investigate the potential protective mechanisms of gintonin in an in vivo MPTP and in vitro MPP[+]-mediated Parkinson's disease (PD) model. We hypothesized that activation of nuclear factor erythroid 2-related factor 2/heme oxygenase-1 (Nrf2/HO-1, potential therapeutic targets for neurodegeneration) with gintonin could abrogate PD-associated neurotoxicity by modulating the accumulation of α-synuclein, neuroinflammation, and apoptotic cell death in an MPTP/MPP[+] models of PD. Our in vivo and in vitro findings suggest that the neuroprotective effects of gintonin were associated with the regulation of the Nrf2/HO-1 pathway, which regulated the expression of proinflammatory cytokines and nitric oxide synthase and apoptotic markers in the substantia nigra and striatum of the mice. Moreover, the neuroprotective effects of gintonin were also associated with a reduction in α-synuclein accumulation in the mouse substantia nigra and striatum. The neuroprotective effects of gintonin were further validated by analyzing the effects of gintonin on MPP[+]-treated SH-SY5Y cells, which confirmed the protective effects of gintonin. It remains for future basic and clinical research to determine the potential use of gintonin in Parkinson's disease. However, to the best of our knowledge, marked alterations in biochemical and morphological setup of midbrain dopaminergic pathways by gintonin in MPTP mice model have not been previously reported. We believe that gintonin might be explored as an important therapeutic agent in the treatment of PD. | 2019 Jan | Molecular neurobiology | No DOI | [
{
"LastName": "Jo",
"FirstName": "Min Gi",
"Affiliation": "Division of Life Science and Applied Life Science (BK21 plus), College of Natural Sciences, Gyeongsang National University, Jinju, 52802, Republic of Korea."
},
{
"LastName": "Ikram",
"FirstName": "Muhammad",
"Affiliation": "Division of Life Science and Applied Life Science (BK21 plus), College of Natural Sciences, Gyeongsang National University, Jinju, 52802, Republic of Korea."
},
{
"LastName": "Jo",
"FirstName": "Myeung Hoon",
"Affiliation": "Division of Life Science and Applied Life Science (BK21 plus), College of Natural Sciences, Gyeongsang National University, Jinju, 52802, Republic of Korea."
},
{
"LastName": "Yoo",
"FirstName": "Lang",
"Affiliation": "Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea."
},
{
"LastName": "Chung",
"FirstName": "Kwang Chul",
"Affiliation": "Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea."
},
{
"LastName": "Nah",
"FirstName": "Seung-Yeol",
"Affiliation": "Ginsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine, Konkuk University, Seoul, 05029, Republic of Korea."
},
{
"LastName": "Hwang",
"FirstName": "Hongik",
"Affiliation": "Center for Neuroscience, Division of Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea."
},
{
"LastName": "Rhim",
"FirstName": "Hyewhon",
"Affiliation": "Center for Neuroscience, Division of Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea. hrhim@kist.re.kr."
},
{
"LastName": "Kim",
"FirstName": "Myeong Ok",
"Affiliation": "Division of Life Science and Applied Life Science (BK21 plus), College of Natural Sciences, Gyeongsang National University, Jinju, 52802, Republic of Korea. mokim@gnu.ac.kr."
}
] | Yes |
18969134 | Analytical determination of homocysteine: a review. | The determination of homocysteine has gained high interest within the biomedical community over recent years as the molecule is an important biomarker for a wide range of diseases. The current report gives a brief overview into the biological significance of homocysteine and a rigorous account of the existing detection protocols for homocysteine in biological fluids, as well as trying to anticipate possible future trends in the development of rapid, low-cost and sensitive assays for its detection. | 2003 Aug 29 | Talanta | No DOI | [
{
"LastName": "Nekrassova",
"FirstName": "Olga",
"Affiliation": "Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK."
},
{
"LastName": "Lawrence",
"FirstName": "Nathan S",
"Affiliation": ""
},
{
"LastName": "Compton",
"FirstName": "Richard G",
"Affiliation": ""
}
] | No |
35795536 | Improved CEEMDAN, GA, and SVR Model for Oil Price Forecasting. | Accurate prediction of crude oil prices (COPs) is a challenge for academia and industry. Therefore, the present research developed a new CEEMDAN-GA-SVR hybrid model to predict COPs, incorporating complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN), a genetic algorithm (GA), and support vector regression machine (SVR). First, our team utilized CEEMDAN to realize the decomposition of a raw series of COPs into a group of comparatively simpler subseries. Second, SVR was utilized to predict values for every decomposed subseries separately. Owing to the intricate parametric settings of SVR, GA was employed to achieve the parametric optimisation of SVR during forecast. Then, our team assembled the forecasted values of the entire subseries as the forecasted values of the CEEMDAN-GA-SVR model. After a series of experiments and comparison of the results, we discovered that the CEEMDAN-GA-SVR model remarkably outperformed single and ensemble benchmark models, as displayed by a case study finished based on a time series of weekly Brent COPs. | 2022 | Journal of environmental and public health | No DOI | [
{
"LastName": "Lu",
"FirstName": "Yichun",
"Affiliation": "School of International Business, Guangxi University, Nanning 530004, China."
},
{
"LastName": "Luo",
"FirstName": "Junyin",
"Affiliation": "School of Economics, Beijing Technology and Business University, Beijing, China."
},
{
"LastName": "Cui",
"FirstName": "Yiwen",
"Affiliation": "School of Public Administration, Hohai University, Nanjing, China."
},
{
"LastName": "He",
"FirstName": "Zhengbin",
"Affiliation": "School of Economics, Beijing Technology and Business University, Beijing, China."
},
{
"LastName": "Xia",
"FirstName": "Fengchun",
"Affiliation": "School of Electrical Engineering, Southwest Minzu University, Chengdu, China."
}
] | Yes |
39007694 | Novel Carbon Quantum Dots Precisely Trigger Ferroptosis in Cancer Cells through Antioxidant Inhibition Synergistic Nanocatalytic Activity. | High levels of glutathione (GSH) are an important characteristic of malignant tumors and a significant cause of ineffective treatment and multidrug resistance. Although reactive oxygen species (ROS) therapy has been shown to induce tumor cell death, the strong clearance effect of GSH on ROS significantly reduces its therapeutic efficacy. Therefore, there is a need to develop new strategies for targeting GSH. In this study, novel carbon quantum dots derived from gentamycin (GM-CQDs) were designed and synthesized. On the basis of the results obtained, GM-CQDs contain sp[2] and sp[3] carbon atoms as well as nitrogen oxygen groups, which decrease the intracellular levels of GSH by downregulating SLC7A11, thereby disrupting redox balance, mediating lipid peroxidation, and inducing ferroptosis. Transcriptome analysis demonstrated that GM-CQDs downregulated the expression of molecules related to GSH metabolism while significantly increasing the expression of molecules related to ferroptosis. The in vivo results showed that the GM-CQDs exhibited excellent antitumor activity and immune activation ability. Furthermore, because of their ideal biological safety, GM-CQDs are highly promising for application as drugs targeting GSH in the treatment of malignant tumors. | 2024 Jul 24 | ACS applied materials & interfaces | No DOI | [
{
"LastName": "Jin",
"FirstName": "Nianqiang",
"Affiliation": "Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, Guangdong 510280, People's Republic of China."
},
{
"LastName": "Wang",
"FirstName": "Zilin",
"Affiliation": "Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, People's Republic of China."
},
{
"LastName": "Yin",
"FirstName": "Chengcheng",
"Affiliation": "Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, Liaoning 110002, People's Republic of China."
},
{
"LastName": "Bu",
"FirstName": "Wenhuan",
"Affiliation": "School and Hospital of Stomatology, Jilin University, Changchun, Jilin 130000, People's Republic of China."
},
{
"LastName": "Jin",
"FirstName": "Nuo",
"Affiliation": "Department of Tissue Engineering, Center of 3D Printing & Organ Manufacturing, School of Fundamental Sciences, China Medical University, Shenyang, Liaoning 110001, People's Republic of China."
},
{
"LastName": "Ou",
"FirstName": "Lingling",
"Affiliation": "Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, Guangdong 510280, People's Republic of China."
},
{
"LastName": "Xie",
"FirstName": "Wenqiang",
"Affiliation": "Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, Guangdong 510280, People's Republic of China."
},
{
"LastName": "He",
"FirstName": "Jiankang",
"Affiliation": "Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, Guangdong 510280, People's Republic of China."
},
{
"LastName": "Lai",
"FirstName": "Xuan",
"Affiliation": "Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, Guangdong 510280, People's Republic of China."
},
{
"LastName": "Shao",
"FirstName": "Longquan",
"Affiliation": "Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, Guangdong 510280, People's Republic of China."
}
] | No |
35310589 | An Algorithm for Time Prediction Signal Interference Detection Based on the LSTM-SVM Model. | Interference detection is an important part of the electronic defense system. It is difficult to detect interference with the traditional method of extracting characteristic parameters for interference generated at the same frequency as the original signal. Aiming at this special time-frequency overlapping interference signal, this paper proposes an interference detection algorithm based on the long short-term memory-support vector machines (LSTM-SVM) model. LSTM is used for the time series prediction of the received signal. The difference between the predicted signal and the received signal is used as the feature sample, and the SVM algorithm is used to classify the feature samples to obtain the recognition rate of whether the sample has interference. The LSTM-SVM model is compared with the gate recurrent unit-support vector machines (GRU-SVM) model, and the comparison results are visualized using a confusion matrix. The simulation results show that this LSTM-SVM model algorithm cannot only detect the existence of the interference signal but also can determine the specific position of the interference signal in the received waveform, and the detection performance is better than the GRU-SVM model. | 2022 | Computational intelligence and neuroscience | No DOI | [
{
"LastName": "Xiao",
"FirstName": "Ningbo",
"Affiliation": "School of Electronics and Information, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, China."
},
{
"LastName": "Song",
"FirstName": "Zuxun",
"Affiliation": "School of Electronics and Information, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, China."
}
] | Yes |
18364932 | Experiments with a genetic algorithm for structural design of cemented doublets with prespecified aberration targets. | The authors use a genetic algorithm in the structural design of cemented doublets in accordance with a prespecified set of Gaussian characteristics and primary aberration targets. Actual glasses are taken as variables. Suitable modifications in the algorithm are sought with which to explore the solution space in search of the global as well as useful local optima. Some illustrative examples of structural design are given. | 2001 Dec 1 | Applied optics | No DOI | [
{
"LastName": "Banerjee",
"FirstName": "S",
"Affiliation": ""
},
{
"LastName": "Hazra",
"FirstName": "L",
"Affiliation": ""
}
] | No |
31802647 | Physcion 8-O-β-glucopyranoside inhibits testicular germ cell tumors through regulating microRNA-199a. | Testicular germ cell tumors (TGCTs) are frequently diagnosed solid tumors in young adult males. The present study aimed to investigate the anti-tumor function of Physcion 8-O-β-Glucopyranoside (PG) in TGCTs, and to explore the underlying anti-tumor mechanism of PG in TGCTs. Cell viability was measured by a cell counting kit-8 (CCK-8) assay, and the cell cycle distribution was analyzed by flow cytometry. Cell apoptosis was measured using TUNEL staining and flow cytometry. The lactate dehydrogenase (LDH) release, glucose consumption, lactate production, and ATP generation were determined using a Flex Bioanalyzer. The expression of miR-199a was measured by qRT-PCR, and the expression of marker proteins were analyzed by western blotting. Moreover, a xenograft mouse model was used for investigating PG's antitumor effect in vivo. The results showed that PG significantly inhibited NTERA2 and NCCIT cell proliferation, blocked the cell cycle, and induced cell apoptosis. Furthermore, PG suppressed LDH release, glucose consumption, lactate production, and ATP generation in NTERA2 and NCCIT cells. The results of investigating the molecular mechanism of PG in TGCTs showed that miR-199a was decreased in TGCTs but was increased by PG in a dose-dependent manner. MiR-199a knockdown significantly increased NTERA2 and NCCIT cell proliferation, decreased the apoptotic cell rates, and promoted LDH release, glucose consumption, lactate production, and ATP generation. The effects of PG on TGCTs were reversed by miR-199a silencing. In addition, we found that PG significantly inhibited tumor growth in vivo. In conclusion, our study revealed that PG modulated cell proliferation, the cell cycle, apoptosis, and glucose metabolism in TGCTs through regulating miR-199a. These findings indicate a novel therapeutic strategy and a potential biomarker for TGCT treatment. | 2020 Dec | Anatomical record (Hoboken, N.J. : 2007) | No DOI | [
{
"LastName": "Dou",
"FirstName": "Xiangyang",
"Affiliation": "Department of Urology, Yan'an University Affiliated Hospital, Yan'an, China."
},
{
"LastName": "Wang",
"FirstName": "Meihua",
"Affiliation": "Department of Hematology and Immunology, Yan'an University Affiliated Hospital, Yan'an, China."
},
{
"LastName": "Zhang",
"FirstName": "Tao",
"Affiliation": "Department of Urology, Ankang in Shaanxi Province Chinese Traditional Medicine Hospital, Ankang, China."
},
{
"LastName": "Yao",
"FirstName": "Jiapei",
"Affiliation": "Department of Urology, First Affiliated Hospital of Kunming Medical University, Kunming, China."
}
] | Yes |
11719801 | Superconductivity in CaCuO2 as a result of field-effect doping. | Understanding the doping mechanisms in the simplest superconducting copper oxide-the infinite-layer compound ACuO2 (where A is an alkaline earth metal)-is an excellent way of investigating the pairing mechanism in high-transition-temperature (high-Tc) superconductors more generally. Gate-induced modulation of the carrier concentration to obtain superconductivity is a powerful means of achieving such understanding: it minimizes the effects of potential scattering by impurities, and of structural modifications arising from chemical dopants. Here we report the transport properties of thin films of the infinite-layer compound CaCuO2 using field-effect doping. At high hole- and electron-doping levels, superconductivity is induced in the nominally insulating material. Maximum values of Tc of 89 K and 34 K are observed respectively for hole- and electron-type doping of around 0.15 charge carriers per CuO2. We can explore the whole doping diagram of the CuO2 plane while changing only a single electric parameter, the gate voltage. | 2001 Nov 22 | Nature | No DOI | [
{
"LastName": "Schön",
"FirstName": "J H",
"Affiliation": "Bell Laboratories, Lucent Technologies, 600 Mountain Avenue, Murray Hill, New Jersey 07974-0636, USA. hendrik@lucent.com"
},
{
"LastName": "Dorget",
"FirstName": "M",
"Affiliation": ""
},
{
"LastName": "Beuran",
"FirstName": "F C",
"Affiliation": ""
},
{
"LastName": "Zu",
"FirstName": "X Z",
"Affiliation": ""
},
{
"LastName": "Arushanov",
"FirstName": "E",
"Affiliation": ""
},
{
"LastName": "Deville Cavellin",
"FirstName": "C",
"Affiliation": ""
},
{
"LastName": "Laguës",
"FirstName": "M",
"Affiliation": ""
}
] | Yes |
33603662 | Anthocyanin Protects Cardiac Function and Cardiac Fibroblasts From High-Glucose Induced Inflammation and Myocardial Fibrosis by Inhibiting IL-17. | Diabetic cardiomyopathy (DCM) is one of the major causes of death in diabetic patients. Its pathogenesis involves inflammation and fibrosis that damages the heart tissue and impairs cardiac function. Interleukin (IL)-17, a pro-inflammatory cytokine that plays an important role in a variety of chronic inflammatory processes can serve as an attractive therapeutic target. Anthocyanin, a water-soluble natural pigment, possesses impressive anti-inflammatory activity. However, its role in DCM is unclear. Hence, we investigated the protective effect of anthocyanin on the cardiovascular complications of diabetes using a mouse type 1 diabetes mellitus model induced by streptozotocin. Cardiac function and structural alterations in diabetic mice were tested by echocardiography, hematoxylin and eosin staining, and Masson trichrome staining. Immunohistochemistry was performed to evaluate the distribution and deposition of IL-17 and collagen I and III from the left ventricular tissues of diabetic mice. Cell viability was measured using the methyl thiazolyl tetrazolium assay. Protein levels of IL-17, tumor necrosis factor α, IL-1β, and IL-6 were determined using enzyme-linked immunosorbent assay. IL-17 and collagen I and III were detected by western blotting and immunofluorescence, and their mRNA levels were quantified using quantitative reverse transcription PCR. We observed that anthocyanin lowered blood glucose, improved cardiac function, and alleviated inflammation and fibrosis in the heart tissue of diabetic mice. Meanwhile, anthocyanin reduced the expression of IL-17 in high-glucose-treated cardiac fibroblasts and exhibited an anti-inflammatory effect. Deposition of collagen I and III was also decreased by anthocyanin, suggesting that anthocyanin contributes to alleviating myocardial fibrosis. In summary, anthocyanin could protect cardiac function and inhibit IL-17-related inflammation and fibrosis, which indicates its therapeutic potential in the treatment of diabetes mellitus-related complications. | 2020 | Frontiers in pharmacology | No DOI | [
{
"LastName": "Yue",
"FirstName": "Er",
"Affiliation": "Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, China."
},
{
"LastName": "Yu",
"FirstName": "Yahan",
"Affiliation": "Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, China."
},
{
"LastName": "Wang",
"FirstName": "Xinyao",
"Affiliation": "Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, China."
},
{
"LastName": "Liu",
"FirstName": "Bing",
"Affiliation": "Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, China."
},
{
"LastName": "Bai",
"FirstName": "Yunlong",
"Affiliation": "Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, China."
},
{
"LastName": "Yang",
"FirstName": "Baofeng",
"Affiliation": "Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, China."
}
] | No |
36631030 | Erratum: Retraction of Figure 1 in the Article "Immediate Hypersensitivity Reactions Induced by Triamcinolone in a Patient With Atopic Dermatitis". | This corrects the article on p. e87 in vol. 33, PMID: 29542298. | 2022 Nov 28 | Journal of Korean medical science | No DOI | [
{
"LastName": "Son",
"FirstName": "Jee Hee",
"Affiliation": "Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, Korea."
},
{
"LastName": "Park",
"FirstName": "Sook Young",
"Affiliation": "Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, Korea."
},
{
"LastName": "Cho",
"FirstName": "Yong Se",
"Affiliation": "Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, Korea."
},
{
"LastName": "Chung",
"FirstName": "Bo Young",
"Affiliation": "Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, Korea."
},
{
"LastName": "Kim",
"FirstName": "Hye One",
"Affiliation": "Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, Korea."
},
{
"LastName": "Park",
"FirstName": "Chun Wook",
"Affiliation": "Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, Korea. dermap@hanmail.net."
}
] | No |
23303244 | Accuracy of noninvasive haemoglobin measurement by pulse oximetry depends on the type of infusion fluid. | Measurement of blood haemoglobin concentration by pulse oximetry could be of value in determining when erythrocytes should be transfused during surgery, but the effect of infusion fluids on the results is unclear. To study the effect of crystalloid and colloid fluid on the accuracy (bias) and precision of pulse oximetry haemoglobin estimation to indicate the venous haemoglobin concentration in volunteers. Open interventional crossover study. Single university hospital. Ten male volunteers aged 18-28 (mean 22) years. Each volunteer underwent three infusion experiments on separate days and in random order. The infusions were Ringer's acetate (20 ml kg), hydroxyethyl starch 130/0.4 (10 ml kg) and a combination of both. At the end of the infusions of Ringer's acetate, pulse oximetry haemoglobin concentration had decreased more than the true haemoglobin concentration (15 vs. 8%; P < 0.005; n = 10) whereas starch solution decreased pulse oximetry haemoglobin concentration less than true haemoglobin concentration (7 vs. 11%; P < 0.02; n = 20). The same differences were seen when the fluids were infused separately and when they were combined. The overall difference between all 956 pairs of pulse oximetry haemoglobin concentration and true haemoglobin concentrations (the bias) averaged only -0.7 g l whereas the 95% prediction interval was wide, ranging from -24.9 to 23.7 g l. In addition to the choice of infusion fluid, the bias was strongly dependent on the volunteer (each factor, P < 0.001). The bias of measuring haemoglobin concentration by pulse oximetry is dependent on whether a crystalloid or a colloid fluid is infused. ClinicalTrials identifier: NCT01195025. | 2013 Feb | European journal of anaesthesiology | No DOI | [
{
"LastName": "Bergek",
"FirstName": "Christian",
"Affiliation": "Section for Anaesthesia, Linköping University, Linköping, Sweden."
},
{
"LastName": "Zdolsek",
"FirstName": "Joachim H",
"Affiliation": ""
},
{
"LastName": "Hahn",
"FirstName": "Robert G",
"Affiliation": ""
}
] | Yes |
24431811 | Percutaneous Radiofrequency Rhizotomy in Treatment of Trigeminal neuralgia: A Prospective Study. | Trigeminal neuralgia (TN) often called as "Tic douloureux" is a syndrome characterized by paroxysmal facial pain, is one of the most painful and debilitating craniofacial pain disorders. The controversy regarding the etiology and treatment of TN still exists. To evaluate the effectiveness of percutaneous radiofrequency rhizotomy (PRR) for TN, after failure of pharmacological management. A nonrandomized, non comparative, descriptive, in vivo study of 15 patients with TN of maxillary and mandibular divisions of trigeminal nerve and patients with pain refractory to pharmacological management were done. It was performed as an O.P.D procedure. A routine follow up was done in all cases for 1 year. In a total of 15 patients, 8 female and 7 male patients were enrolled for the study. Early pain relief (immediately, postoperatively to 6 month) classified as excellent or good (successful), occurred in 12 of 15 patients (80 %). Fair or poor pain relief (unsuccessful) occurred in three patients (20 %).There was no mortalities and no major morbidity. It was found that surgical treatment with PRR is a safe and effective way to manage patients with TN in whom pharmacologic therapy is either ineffective or not tolerated, with low side effects which are well tolerated. | 2013 Mar | Journal of maxillofacial and oral surgery | No DOI | [
{
"LastName": "Nanjappa",
"FirstName": "Madan",
"Affiliation": "Department of Oral & Maxillofacial Surgery, V. S. Dental College & Hospital, K R Road, VV Puram, Bangalore, 560004 India."
},
{
"LastName": "Kumaraswamy",
"FirstName": "S V",
"Affiliation": "Department of Oral & Maxillofacial Surgery, V. S. Dental College & Hospital, K R Road, VV Puram, Bangalore, 560004 India."
},
{
"LastName": "Keerthi",
"FirstName": "R",
"Affiliation": "Department of Oral & Maxillofacial Surgery, V. S. Dental College & Hospital, K R Road, VV Puram, Bangalore, 560004 India."
},
{
"LastName": "Ashwin",
"FirstName": "D P",
"Affiliation": "Department of Oral & Maxillofacial Surgery, V. S. Dental College & Hospital, K R Road, VV Puram, Bangalore, 560004 India."
},
{
"LastName": "Gopinath",
"FirstName": "A L",
"Affiliation": "Department of Oral & Maxillofacial Surgery, V. S. Dental College & Hospital, K R Road, VV Puram, Bangalore, 560004 India."
},
{
"LastName": "Reyazulla",
"FirstName": "M A",
"Affiliation": "Department of Oral & Maxillofacial Surgery, V. S. Dental College & Hospital, K R Road, VV Puram, Bangalore, 560004 India."
},
{
"LastName": "Hemavathi",
"FirstName": "K B",
"Affiliation": "Department of Oral & Maxillofacial Surgery, V. S. Dental College & Hospital, K R Road, VV Puram, Bangalore, 560004 India."
}
] | No |
7543632 | Autolymphocyte therapy. III. Effective adjuvant adoptive cellular therapy with in vivo anti-tumor specificity against murine melanoma and carcinoma using ex-vivo-activated memory T-lymphocytes. | Autolymphocyte therapy (ALT) is adoptive cellular therapy of neoplastic disease based upon ex vivo activation of lymphocytes by either the supernatant derived from a previously prepared one-way mixed lymphocyte culture (MLC) or using low doses of the mitogenic monoclonal antibody OKT3 and a mixture of previously prepared cytokines (T3CS). We have previously demonstrated that nonspecific ex vivo activation of splenocytes from murine tumor-bearing hosts (TBH) using an MLC-supernatant or T3CS without the use of tumor antigen results in the expansion of the CD44+ (memory) T-cell subset. These CD44+ T-cells are the principal mediators of anti-tumor specificity in the ALT-cell population in advanced metastatic murine tumors and are able to protect against tumor challenge in healthy syngeneic mice (HSM). To determine if ALT is effective in an adjuvant setting, C57BL/6J splenocytes from HSM and TBH with B16 melanoma or Lewis lung (3LL) carcinoma were activated ex vivo using T3CS. Mice were implanted with either B16 melanoma or 3LL carcinoma and then underwent surgical excision of tumor. Tumor-excised mice (TEM) then received small numbers (10(6)) of ALT-cells derived from 3LL-TBH or B16-TBH splenocytes, HSM-derived ALT-cells, fresh splenocytes derived from 3LL-TBH or B16-TBH, or CD44-depleted ALT-cells. Significant anti-tumor activity as shown by prolonged survival (Day 100), cure of disease, and rejection of a local and systemic tumor rechallenge was demonstrated in 3LL-TEM that received B16-derived ALT-cells.(ABSTRACT TRUNCATED AT 250 WORDS) | 1995 Aug | The Journal of surgical research | No DOI | [
{
"LastName": "Gold",
"FirstName": "J E",
"Affiliation": "Department of Medicine, Mount Sinai Hospital, New York, New York, USA."
},
{
"LastName": "Masters",
"FirstName": "T R",
"Affiliation": ""
},
{
"LastName": "Osband",
"FirstName": "M E",
"Affiliation": ""
}
] | Yes |
19298132 | The oncopathic potency of Clostridium perfringens is independent of its alpha-toxin gene. | Hypoxia in solid tumors is a major obstacle in conventional treatment because of inefficient delivery of therapeutic agents to the lesions, but offers the potential for anaerobic bacterial colonization that can lead to tumor destruction. We have previously reported a recombinant Clostridium perfringens (Cp) strain constructed by deletion of the superoxide dismutase (sod) gene and insertion of the Panton-Valentine leukocidin (PVL) gene, Cp/sod(-)/PVL, which showed elevated oxygen sensitivity, tumor selectivity, and oncopathic potency in an orthotopic model of pancreatic cancer in immune-competent and syngeneic mice, and that led to substantial prolongation of animal survival. A major limitation to Cp/sod(-)/PVL in clinical applications is that it expresses phospholipase C (plc), the alpha-toxin and the major virulence determinant in Cp that is causative in the development of gas gangrene. In this study, the plc gene in Cp/sod(-)/PVL was knocked out to create Cp/plc(-)/sod(-)/PVL, which was shown to be incapable of inducing gas gangrene in mice. Intravenous injection of Cp/plc(-)/sod(-)/PVL spores led to a significant survival advantage in tumor-bearing mice with the same efficacy as Cp/sod(-)/PVL, indicating that the oncopathic potency of Cp is independent of a functional plc gene. The treatment also did not lead to an attenuated immune response to a subsequent pathogen challenge, indicating that a systemic immune-suppressive effect in the host is absent. Consequently, Cp/plc(-)/sod(-)/PVL is a novel oncopathic bacterial agent for the effective treatment of pancreatic cancer and other poorly vascularized tumors, with a substantially enhanced safety profile, which is essential for the development of translational studies in the future. | 2009 Jul | Human gene therapy | No DOI | [
{
"LastName": "Li",
"FirstName": "Zhiyu",
"Affiliation": "Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA."
},
{
"LastName": "Fallon",
"FirstName": "John",
"Affiliation": ""
},
{
"LastName": "Mandeli",
"FirstName": "John",
"Affiliation": ""
},
{
"LastName": "Wetmur",
"FirstName": "James",
"Affiliation": ""
},
{
"LastName": "Woo",
"FirstName": "Savio L C",
"Affiliation": ""
}
] | Yes |
38301006 | Local activation of CA1 pyramidal cells induces theta-phase precession. | Hippocampal theta-phase precession is involved in spatiotemporal coding and in generating multineural spike sequences, but how precession originates remains unresolved. To determine whether precession can be generated directly in hippocampal area CA1 and disambiguate multiple competing mechanisms, we used closed-loop optogenetics to impose artificial place fields in pyramidal cells of mice running on a linear track. More than one-third of the CA1 artificial fields exhibited synthetic precession that persisted for a full theta cycle. By contrast, artificial fields in the parietal cortex did not exhibit synthetic precession. These findings are incompatible with precession models based on inheritance, dual-input, spreading activation, inhibition-excitation summation, or somato-dendritic competition. Thus, a precession generator resides locally within CA1. | 2024 Feb 2 | Science (New York, N.Y.) | No DOI | [
{
"LastName": "Sloin",
"FirstName": "Hadas E",
"Affiliation": "Sagol School of Neuroscience, Tel Aviv University, Tel Aviv 6997801, Israel."
},
{
"LastName": "Spivak",
"FirstName": "Lidor",
"Affiliation": "Sagol School of Neuroscience, Tel Aviv University, Tel Aviv 6997801, Israel."
},
{
"LastName": "Levi",
"FirstName": "Amir",
"Affiliation": "Sagol School of Neuroscience, Tel Aviv University, Tel Aviv 6997801, Israel."
},
{
"LastName": "Gattegno",
"FirstName": "Roni",
"Affiliation": "Sagol School of Neuroscience, Tel Aviv University, Tel Aviv 6997801, Israel."
},
{
"LastName": "Someck",
"FirstName": "Shirly",
"Affiliation": "Sagol School of Neuroscience, Tel Aviv University, Tel Aviv 6997801, Israel."
},
{
"LastName": "Stark",
"FirstName": "Eran",
"Affiliation": "Sagol School of Neuroscience, Tel Aviv University, Tel Aviv 6997801, Israel."
}
] | No |
32038319 | Toward A Greater Understanding of the Ways Family-Based Treatment Addresses the Full Range of Psychopathology of Adolescent Anorexia Nervosa. | Family-based treatment (FBT) for anorexia nervosa (AN) is an empirically supported treatment for this disorder. Derived from several different schools of family therapy, it is a highly focused approach that initially targets weight restoration under parental management at home. However, the view that manualized FBT is solely a behavioral therapy directing parents to refeed their children AN with the single purpose of weight gain is a common but misleading over simplification of the therapy. Indeed, weight restoration is the main goal only in phase 1 of this 3-phase treatment. When practiced with fidelity and skill, FBT's broadest aim is to promote adolescent development without AN thoughts and behaviors interfering and disrupting these normal processes. Although weight restoration is a key starting point in FBT, the entire course of treatment takes into consideration the ongoing impact of starvation, cognitions, emotions, and behaviors on adolescent development. These factors associated with maintaining low weight are viewed in FBT as interfering with the adolescent being able to take up the tasks of adolescence and thus must be overcome before fully turning to those broader adolescent tasks. In addition, FBT recognizes that adolescence takes place in the context of family and community and respects the importance of learning in a home environment both for weight gain as well as related developmental tasks to have a lasting effect. Specifically, in this article we describe how the current FBT manualized approach addresses temperament/personality traits, emotional processing, cognitive content and process, social communication and connections, psychiatric comorbidity, and family factors. This report makes no claim to superiority of FBT compared to other therapies in addressing these broader concerns nor does it add interventions to augment the current manual to improve FBT. | 2019 | Frontiers in psychiatry | No DOI | [
{
"LastName": "Lock",
"FirstName": "James",
"Affiliation": "Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, United States."
},
{
"LastName": "Nicholls",
"FirstName": "Dasha",
"Affiliation": "Division of Psychiatry, Department of Brain Sciences, Imperial College London, London, United Kingdom."
}
] | No |
33299470 | Multichannel Saliency Detection Based on Visual Bionics. | Inspired by the visual properties of the human eyes, the depth information of visual attention is integrated into the saliency detection to effectively solve problems such as low accuracy and poor stability under similar or complex background interference. Firstly, the improved SLIC algorithm was used to segment and cluster the RGBD image. Secondly, the depth saliency of the image region was obtained according to the anisotropic center-surround difference method. Then, the global feature saliency of RGB image was calculated according to the colour perception rule of human vision. The obtained multichannel saliency maps were weighted and fused based on information entropy to highlighting the target area and get the final detection results. The proposed method works within a complexity of O(N), and the experimental results show that our algorithm based on visual bionics effectively suppress the interference of similar or complex background and has high accuracy and stability. | 2020 | Applied bionics and biomechanics | No DOI | [
{
"LastName": "Cheng",
"FirstName": "Lidan",
"Affiliation": "College of Optoelectronics Science and Engineering, Soochow University, Suzhou Jiangsu Province 215000, China."
},
{
"LastName": "Li",
"FirstName": "Tianyi",
"Affiliation": "College of Optoelectronics Science and Engineering, Soochow University, Suzhou Jiangsu Province 215000, China."
},
{
"LastName": "Zha",
"FirstName": "Shijia",
"Affiliation": "College of Optoelectronics Science and Engineering, Soochow University, Suzhou Jiangsu Province 215000, China."
},
{
"LastName": "Wei",
"FirstName": "Wei",
"Affiliation": "College of Optoelectronics Science and Engineering, Soochow University, Suzhou Jiangsu Province 215000, China."
},
{
"LastName": "Gu",
"FirstName": "Jihua",
"Affiliation": "College of Optoelectronics Science and Engineering, Soochow University, Suzhou Jiangsu Province 215000, China."
}
] | No |
37341483 | Topology and function of translocated EspZ. | EspZ and Tir are essential virulence effectors of enteropathogenic Escherichia coli (EPEC). EspZ, the second translocated effector, has been suggested to antagonize host cell death induced by the first translocated effector, Tir (translocated intimin receptor). Another characteristic of EspZ is its localization to host mitochondria. However, studies that explored the mitochondrial localization of EspZ have examined the ectopically expressed effector and not the more physiologically relevant translocated effector. Here, we confirmed the membrane topology of translocated EspZ at infection sites and the involvement of Tir in confining its localization to these sites. Unlike the ectopically expressed EspZ, the translocated EspZ did not colocalize with mitochondrial markers. Moreover, no correlation has been found between the capacity of ectopically expressed EspZ to target mitochondria and the ability of translocated EspZ to protect against cell death. Translocated EspZ may have to some extent diminished F-actin pedestal formation induced by Tir but has a marked effect on protecting against host cell death and on promoting host colonization by the bacteria. Taken together, our results suggest that EspZ plays an essential role in facilitating bacterial colonization, likely by antagonizing cell death mediated by Tir at the onset of bacterial infection. This activity of EspZ, which occurs by targeting host membrane components at infection sites, and not mitochondria, may contribute to successful bacterial colonization of the infected intestine. IMPORTANCE EPEC is an important human pathogen that causes acute infantile diarrhea. EspZ is an essential virulence effector protein translocated from the bacterium into the host cells. Detailed knowledge of its mechanisms of action is, therefore, critical for better understanding the EPEC disease. We show that Tir, the first translocated effector, confines the localization of EspZ, the second translocated effector, to infection sites. This activity is important for antagonizing the pro-cell death activity conferred by Tir. Moreover, we show that translocated EspZ leads to effective bacterial colonization of the host. Hence, our data suggest that translocated EspZ is essential because it confers host cell survival to allow bacterial colonization at an early stage of bacterial infection. It performs these activities by targeting host membrane components at infection sites. Identifying these targets is critical for elucidating the molecular mechanism underlying the EspZ activity and the EPEC disease. | 2023 Aug 31 | mBio | No DOI | [
{
"LastName": "Haritan",
"FirstName": "Nir",
"Affiliation": "Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem, Israel."
},
{
"LastName": "Bouman",
"FirstName": "Etan Amse",
"Affiliation": "Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem, Israel."
},
{
"LastName": "Nandi",
"FirstName": "Ipsita",
"Affiliation": "Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem, Israel."
},
{
"LastName": "Shtuhin-Rahav",
"FirstName": "Raisa",
"Affiliation": "Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem, Israel."
},
{
"LastName": "Zlotkin-Rivkin",
"FirstName": "Efrat",
"Affiliation": "Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem, Israel."
},
{
"LastName": "Danieli",
"FirstName": "Tsafi",
"Affiliation": "The Protein Production Facility, Wolfson Centre for Applied Structural Biology, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem, Israel."
},
{
"LastName": "Melamed-Book",
"FirstName": "Naomi",
"Affiliation": "Bioimaging Unit, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem, Israel."
},
{
"LastName": "Nir-Keren",
"FirstName": "Yael",
"Affiliation": "The Protein Production Facility, Wolfson Centre for Applied Structural Biology, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem, Israel."
},
{
"LastName": "Aroeti",
"FirstName": "Benjamin",
"Affiliation": "Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem, Israel."
}
] | No |
16138565 | Association of angiotensin converting enzyme gene polymorphisms with left ventricular hypertrophy. | The angiotensin converting enzyme gene (ACE) is of much interest as a candidate gene conferring an individual's genetic susceptibility to left ventricular hypertrophy (LVH). LVH has long been thought to be an end point of essential hypertension (EH), rather than a separate entity, though it is influenced by a unique set of hormonal, vascular and genetic factors. In this study, we attempted to determine whether two representative polymorphisms of the ACE gene, ACE I/D and 2350 G>A, known to be associated with EH and to have a highly significant influence on plasma ACE levels, could implicate ACE as a quantitative trait locus (QTL) for LVH. We carried out a retrospective, case-control study of the two ACE polymorphisms amongst 180 nationals (50 LVH patients and 130 controls) from the United Arab Emirates (Emirati)--an ethnic group characterized by an absence of alcohol intake and cigarette smoking--for putative correlations with LVH. Clinical diagnoses of LVH were based on echocardiographic and ECG criteria. ACE I/D and 2350 G>A genotypes were determined by polymerase chain reaction (PCR) and restriction digestion. Univariate and multivariate logistic regression analyses revealed an association between ACE polymorphisms and LVH. Haplotype analysis further supported this finding. The ACE I/D and ACE 2350 G>A polymorphisms were in strong linkage disequilibrium and were independently associated with LVH, suggesting that ACE is likely to be a QTL for LVH. In conclusion, This is the first association study of the ACE 2350 G>A polymorphism with LVH; the results showed that this polymorphism, along with ACE I/D, is associated with LVH. | 2005 Apr | Hypertension research : official journal of the Japanese Society of Hypertension | No DOI | [
{
"LastName": "Saeed",
"FirstName": "Mohammad",
"Affiliation": "From the Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan."
},
{
"LastName": "Saleheen",
"FirstName": "Danish",
"Affiliation": ""
},
{
"LastName": "Siddiqui",
"FirstName": "Sammer",
"Affiliation": ""
},
{
"LastName": "Khan",
"FirstName": "Aisha",
"Affiliation": ""
},
{
"LastName": "Butt",
"FirstName": "Zahid A",
"Affiliation": ""
},
{
"LastName": "Frossard",
"FirstName": "Philippe M",
"Affiliation": ""
}
] | Yes |
38288673 | Engineering, applications, and future perspectives of GPCR-based genetically encoded fluorescent indicators for neuromodulators. | This review explores the evolving landscape of G-protein-coupled receptor (GPCR)-based genetically encoded fluorescent indicators (GEFIs), with a focus on their development, structural components, engineering strategies, and applications. We highlight the unique features of this indicator class, emphasizing the importance of both the sensing domain (GPCR structure and activation mechanism) and the reporting domain (circularly permuted fluorescent protein (cpFP) structure and fluorescence modulation). Further, we discuss indicator engineering approaches, including the selection of suitable cpFPs and expression systems. Additionally, we showcase the diversity and flexibility of their application by presenting a summary of studies where such indicators were used. Along with all the advantages, we also focus on the current limitations as well as common misconceptions that arise when using these indicators. Finally, we discuss future directions in indicator engineering, including strategies for screening with increased throughput, optimization of the ligand-binding properties, structural insights, and spectral diversity. | 2024 Mar | Journal of neurochemistry | No DOI | [
{
"LastName": "Rohner",
"FirstName": "Valentin Lu",
"Affiliation": "Institute of Pharmacology and Toxicology, University of Zürich, Zürich, Switzerland."
},
{
"LastName": "Lamothe-Molina",
"FirstName": "Paul J",
"Affiliation": "Institute of Pharmacology and Toxicology, University of Zürich, Zürich, Switzerland."
},
{
"LastName": "Patriarchi",
"FirstName": "Tommaso",
"Affiliation": "Institute of Pharmacology and Toxicology, University of Zürich, Zürich, Switzerland."
}
] | No |
35704072 | Nickel oxide nanoparticles synthesis using plant extract and evaluation of their antibacterial effects on Streptococcus mutans. | Dental decay is known in the world as the most common human infectious disease. Ascending process of dental caries index in the world shows the failure of oral disease prevention. Streptococcus mutans bacteria cause acid damage and tooth decay by producing acid over time. Nanomaterials with suitable functionality, high permeability, extremely large surface area, significant reactivity, unique mechanical features, and non-bacterial resistance can be considered as promising agents for antimicrobial and antiviral applications. In this study, nickel oxide (NiO) nanoparticles with size range from 2 to 16 nm containing Stevia natural sweetener were eco-friendly synthesized via a simple method. Additionally, their various concentrations were evaluated on S. mutans bacteria by applying the broth dilution method. The results demonstrated that these spherical NiO nanoparticles had efficient bacteriostatic activity on this gram-positive coccus. | 2022 Jul | Bioprocess and biosystems engineering | No DOI | [
{
"LastName": "Moghadam",
"FirstName": "Nastaran Chokhachi Zadeh",
"Affiliation": "Department of Pediatric Dentistry, Boston University Henry M. Goldman School of Dental Medicine, Boston, MA, USA."
},
{
"LastName": "Jasim",
"FirstName": "Saade Abdalkareem",
"Affiliation": "Medical Laboratory Techniques Department, Al-Maarif University College, Al-Anbar-Ramadi, Iraq."
},
{
"LastName": "Ameen",
"FirstName": "Fuad",
"Affiliation": "Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia."
},
{
"LastName": "Alotaibi",
"FirstName": "Dalal H",
"Affiliation": "Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh, 11545, Saudi Arabia."
},
{
"LastName": "Nobre",
"FirstName": "Marcos A L",
"Affiliation": "São Paulo State University (Unesp), School of Technology and Sciences, Presidente Prudente, SP, 19060-900, Brazil."
},
{
"LastName": "Sellami",
"FirstName": "Hanen",
"Affiliation": "Water Research and Technologies Center (CERTE), Borj-Cedria Technopark, University of Carthage, 8020, Soliman, Tunisia."
},
{
"LastName": "Khatami",
"FirstName": "Mehrdad",
"Affiliation": "Antibacterial Materials R&D Centre, China Metal New Materials (Huzhou) Institute, Huzhou, Zhejiang, China. mehrdad7khatami@gmail.com."
}
] | Yes |
34912014 | A brief comparative examination of tangent hyperbolic hybrid nanofluid through a extending surface: numerical Keller-Box scheme. | A novel hybrid nanofluid was explored in order to find an efficient heat-transmitting fluid to replace standard fluids and revolutionary nanofluids. By using tangent hyperbolic hybrid combination nanoliquid with non-Newtonian ethylene glycol (EG) as a basis fluid and a copper (Cu) and titanium dioxide (TiO2) mixture, this work aims to investigate the viscoelastic elements of the thermal transferring process. Flow and thermal facts, such as a slippery extended surface with magnetohydrodynamic (MHD), suction/injection, form factor, Joule heating, and thermal radiation effects, including changing thermal conductivity, were also integrated. The Keller-Box method was used to perform collective numerical computations of parametric analysis using governing equivalences. In the form of graphs and tables, the results of TiO2-Cu/EG hybrid nanofluid were compared to those of standard Cu/EG nanofluid in important critical physical circumstances. The entropy generation study was used to examine energy balance and usefulness for important physically impacting parameters. Detailed scrutiny on entropy development get assisted with Weissenberg number, magnetic parameter, fractional volumes, injection parameter, thermal radiation, variable thermal conductivity, Biot number, shape variation parameter, Reynolds and Brinkman number. Whereas the entropy gets resisted for slip and suction parameter. In this case, spotted entropy buildup with important parametric ranges could aid future optimization. | 2021 Dec 15 | Scientific reports | No DOI | [
{
"LastName": "Jamshed",
"FirstName": "Wasim",
"Affiliation": "Department of Mathematics, Capital University of Science and Technology (CUST), Islamabad, 44000, Pakistan. wasiktk@hotmail.com."
},
{
"LastName": "Prakash",
"FirstName": "M",
"Affiliation": "Department of Mathematics, Dr. N.G.P. Institute of Technology, Coimbatore, 641048, India."
},
{
"LastName": "Devi",
"FirstName": "S Suriya Uma",
"Affiliation": "Department of Mathematics, KPR Institute of Engineering and Technology, Coimbatore, 641407, India."
},
{
"LastName": "Ibrahim",
"FirstName": "Rabha W",
"Affiliation": "IEEE: 94086547, 59200, Kuala Lumpur, Malaysia."
},
{
"LastName": "Shahzad",
"FirstName": "Faisal",
"Affiliation": "Department of Mathematics, College of Arts and Sciences, Prince Sattam Bin Abdulaziz University, Wadi Aldawaser, 11991, Saudi Arabia."
},
{
"LastName": "Nisar",
"FirstName": "Kottakkaran Sooppy",
"Affiliation": "Department of Mathematics, College of Arts and Sciences, Prince Sattam Bin Abdulaziz University, Wadi Aldawaser, 11991, Saudi Arabia."
},
{
"LastName": "Eid",
"FirstName": "Mohamed R",
"Affiliation": "Department of Mathematics, Faculty of Science, New Valley University, Al-Kharga, 72511, Al-Wadi Al-Gadid, Egypt."
},
{
"LastName": "Abdel-Aty",
"FirstName": "Abdel-Haleem",
"Affiliation": "Department of Physics, College of Sciences, University of Bisha, P.O. Box 344, Bisha, 61922, Saudi Arabia."
},
{
"LastName": "Khashan",
"FirstName": "M Motawi",
"Affiliation": "Department of Basic Sciences, Common First Year, King Saud University, Riyadh, 11451, Saudi Arabia."
},
{
"LastName": "Yahia",
"FirstName": "I S",
"Affiliation": "Laboratory of Nano-Smart Materials for Science and Technology (LNSMST), Department of Physics, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, Saudi Arabia."
}
] | Yes |
33584671 | Vaccination and Infection of Swine With Salmonella Typhimurium Induces a Systemic and Local Multifunctional CD4(+) T-Cell Response. | The gram-negative facultative intracellular bacteria Salmonella Typhimurium (STM) often leads to subclinical infections in pigs, but can also cause severe enterocolitis in this species. Due to its high zoonotic potential, the pathogen is likewise dangerous for humans. Vaccination with a live attenuated STM strain (Salmoporc) is regarded as an effective method to control STM infections in affected pig herds. However, information on the cellular immune response of swine against STM is still scarce. In this study, we investigated the T-cell immune response in pigs that were vaccinated twice with Salmoporc followed by a challenge infection with a virulent STM strain. Blood- and organ-derived lymphocytes (spleen, tonsils, jejunal and ileocolic lymph nodes, jejunum, ileum) were stimulated in vitro with heat-inactivated STM. Subsequently, CD4[+] T cells present in these cell preparations were analyzed for the production of IFN-γ, TNF-α, and IL-17A by flow cytometry and Boolean gating. Highest frequencies of STM-specific cytokine-producing CD4[+] T cells were found in lamina propria lymphocytes of jejunum and ileum. Significant differences of the relative abundance of cytokine-producing phenotypes between control group and vaccinated + infected animals were detected in most organs, but dominated in gut and lymph node-residing CD4[+] T cells. IL-17A producing CD4[+] T cells dominated in gut and gut-draining lymph nodes, whereas IFN-γ/TNF-α co-producing CD4[+] T cells were present in all locations. Additionally, the majority of cytokine-producing CD4[+] T cells had a CD8α[+]CD27[-] phenotype, indicative of a late effector or effector memory stage of differentiation. In summary, we show that Salmonella-specific multifunctional CD4[+] T cells exist in vaccinated and infected pigs, dominate in the gut and most likely contribute to protective immunity against STM in the pig. | 2020 | Frontiers in immunology | No DOI | [
{
"LastName": "Schmidt",
"FirstName": "Selma",
"Affiliation": "Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Sassu",
"FirstName": "Elena L",
"Affiliation": "University Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Vatzia",
"FirstName": "Eleni",
"Affiliation": "Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Pierron",
"FirstName": "Alix",
"Affiliation": "Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Lagler",
"FirstName": "Julia",
"Affiliation": "Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Mair",
"FirstName": "Kerstin H",
"Affiliation": "Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Stadler",
"FirstName": "Maria",
"Affiliation": "Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Knecht",
"FirstName": "Christian",
"Affiliation": "University Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Spergser",
"FirstName": "Joachim",
"Affiliation": "Institute of Microbiology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Dolezal",
"FirstName": "Marlies",
"Affiliation": "Platform for Bioinformatics and Biostatistics, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Springer",
"FirstName": "Sven",
"Affiliation": "Ceva Innovation Center GmbH, Dessau-Roßlau, Germany."
},
{
"LastName": "Theuß",
"FirstName": "Tobias",
"Affiliation": "Ceva Innovation Center GmbH, Dessau-Roßlau, Germany."
},
{
"LastName": "Fachinger",
"FirstName": "Vicky",
"Affiliation": "Ceva Innovation Center GmbH, Dessau-Roßlau, Germany."
},
{
"LastName": "Ladinig",
"FirstName": "Andrea",
"Affiliation": "University Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Saalmüller",
"FirstName": "Armin",
"Affiliation": "Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria."
},
{
"LastName": "Gerner",
"FirstName": "Wilhelm",
"Affiliation": "Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria."
}
] | No |
23316116 | Pilot Implementation and Preliminary Evaluation of START:AV Assessments in Secure Juvenile Correctional Facilities. | The Short-Term Assessment of Risk and Treatability: Adolescent Version (START:AV) is a new structured professional judgment guide for assessing short-term risks in adolescents. The scheme may be distinguished from other youth risk assessment and treatment planning instruments by its inclusion of 23 dynamic factors that are each rated for both vulnerability and strength. In addition, START:AV is also unique in that it focuses on multiple adverse outcomes-namely, violence, self-harm, suicide, unauthorized leave, substance abuse, self-neglect, victimization, and general offending-over the short-term (i.e., weeks to months) rather than long-term (i.e., years). This paper describes a pilot implementation and preliminary evaluation of START:AV in three secure juvenile correctional facilities in the southern United States. Specifically, we examined the descriptive characteristics and psychometric properties of START:AV assessments completed by 21 case managers on 291 adolescent offenders (250 boys and 41 girls) at the time of admission. Results provide preliminary support for the feasibility of completing START:AV assessments as part of routine practice. Findings also highlight differences in the characteristics of START:AV assessments for boys and girls and differential associations between the eight START:AV risk domains. Though results are promising, further research is needed to establish the reliability and validity of START:AV assessments completed in the field. | 2012 | International journal of forensic mental health | No DOI | [
{
"LastName": "Desmarais",
"FirstName": "Sarah L",
"Affiliation": "North Carolina State University, Psychology, Raleigh, NC United States."
},
{
"LastName": "Sellers",
"FirstName": "Brian G",
"Affiliation": ""
},
{
"LastName": "Viljoen",
"FirstName": "Jodi L",
"Affiliation": ""
},
{
"LastName": "Cruise",
"FirstName": "Keith R",
"Affiliation": ""
},
{
"LastName": "Nicholls",
"FirstName": "Tonia L",
"Affiliation": ""
},
{
"LastName": "Dvoskin",
"FirstName": "Joel A",
"Affiliation": ""
}
] | No |
22895988 | Interventions for tobacco use prevention in Indigenous youth. | Tobacco use in Indigenous populations (people who have inhabited a country for thousands of years) is often double that in the non-Indigenous population. Addiction to nicotine usually begins during early adolescence and young people who reach the age of 18 as non-smokers are unlikely to become smokers thereafter. Indigenous youth in particular commence smoking at an early age, and a disproportionate burden of substance-related morbidity and mortality exists as a result. To evaluate the effectiveness of intervention programmes to prevent tobacco use initiation or progression to regular smoking amongst young Indigenous populations and to summarise these approaches for future prevention programmes and research. The Cochrane Tobacco Addiction Group Specialised Register was searched in November 2011, with additional searches run in MEDLINE. Online clinical trial databases and publication references were also searched for potential studies. We included randomized and non-randomized controlled trials aiming to prevent tobacco use initiation or progression from experimentation to regular tobacco use in Indigenous youth. Interventions could include school-based initiatives, mass media, multi-component community level interventions, family-based programmes or public policy. Data pertaining to methodology, participants, interventions and outcomes were extracted by one reviewer and checked by a second, whilst information on risk of bias was extracted independently by a combination of two reviewers. Studies were assessed by qualitative narrative synthesis, as insufficient data were available to conduct a meta-analysis. The review process was examined by an Indigenous (Aboriginal) Australian for applicability, acceptability and content. Two studies met all of the eligibility criteria for inclusion within the review and a third was identified as ongoing. The two included studies employed multi-component community-based interventions tailored to the specific cultural aspects of the population and were based in Native American populations (1505 subjects in total). No difference was observed in weekly smoking at 42 months follow-up in the one study assessing this outcome (skills-community group versus control: risk ratio [RR] 0.95, 95% CI 0.78 to 1.14; skills-only group versus control: RR 0.86, 95% CI 0.71 to 1.05). For smokeless tobacco use, no difference was found between the skills-community arm and the control group at 42 weeks (RR 0.93, 95% CI 0.67 to 1.30), though a significant difference was observed between the skills-only arm and the control group (RR 0.57, 95% CI 0.39 to 0.85). Whilst the second study found positive changes for tobacco use in the intervention arm at post test (p < 0.05), this was not maintained at six month follow-up (change score -0.11 for intervention and 0.07 for control). Both studies were rated as high or unclear risk of bias in seven or more domains (out of a total of 10). Based on the available evidence, a conclusion cannot be drawn as to the efficacy of tobacco prevention initiatives tailored for Indigenous youth. This review highlights the paucity of data and the need for more research in this area. Smoking prevalence in Indigenous youth is twice that of the non-Indigenous population, with tobacco experimentation commencing at an early age. As such, a significant health disparity exists where Indigenous populations, a minority, are over-represented in the burden of smoking-related morbidity and mortality. Methodologically rigorous trials are needed to investigate interventions aimed at preventing the uptake of tobacco use amongst Indigenous youth and to assist in bridging the gap between tobacco-related health disparities in Indigenous and non-Indigenous populations. | 2012 Aug 15 | The Cochrane database of systematic reviews | No DOI | [
{
"LastName": "Carson",
"FirstName": "Kristin V",
"Affiliation": "Clinical Practice Unit, The Queen Elizabeth Hospital, Adelaide, South Australia, Australia. kristin.carson@health.sa.gov.au."
},
{
"LastName": "Brinn",
"FirstName": "Malcolm P",
"Affiliation": ""
},
{
"LastName": "Labiszewski",
"FirstName": "Nadina A",
"Affiliation": ""
},
{
"LastName": "Peters",
"FirstName": "Matthew",
"Affiliation": ""
},
{
"LastName": "Chang",
"FirstName": "Anne B",
"Affiliation": ""
},
{
"LastName": "Veale",
"FirstName": "Antony",
"Affiliation": ""
},
{
"LastName": "Esterman",
"FirstName": "Adrian J",
"Affiliation": ""
},
{
"LastName": "Smith",
"FirstName": "Brian J",
"Affiliation": ""
}
] | Yes |
35211192 | Effects of Neonatal Feeding Patterns on Infant Health. | Early and reasonable feeding mode can not only ensure the good nutritional condition of infants but also enhance the infant immunity and resistance, which has a certain role in promoting health and preventing the occurrence of chronic diseases in adulthood. In this study, the effect of neonatal feeding methods on the infant body, the influence of early feeding on the growth rate, and development of infants aged 0-3. From January 2016 to June 2019, a total of 600 infants who have been systematically managed in our hospital since birth were selected. All newborns were 37-40 weeks old, weighing 2350-4100 g, without congenital diseases. They were grouped according to feeding methods. 194 infants were exclusively breastfed, 32.3%; 207 people were artificially fed, 34.5%; 199 people were mixed fed, 33.2%. The Kaup index method was used to judge the nutritional status. Developmental Screening Test for ages 0 to 6 (DST) and Bailey Infant Development Scale (BIDS) were used to rate the intellectual and behavioral development of infants and children. According to the growth and development reference standard of 0 ~ 4-year-old children recommended by who in 2006, the age weight standard deviation score (WAZ), age length (height) standard deviation score (HAZ), and body (long) height-weight standard deviation score (WHZ) were performed, and the statistical analysis was performed with SPSS24.0 software. The height, weight, intelligence, and motor development of infants in the three groups at 1 month, 3 months, 6 months, 12 months, 24 months, and 36 months were analyzed and compared, and the test results were recorded in detail. There was no significant difference in 3-month-old WAZ and HAZ among the three groups (P > 0.05). Within 6 months, the WAZ and HAZ values of the pure breastfeeding group and mixed feeding group were higher than those of the artificial feeding group (P < 0.05). From 6 months to 12 months, the WAZ and HAZ values of the artificial feeding group were better than those of artificial feeding group and mixed feeding group (P < 0.05). The scores of MI, DQ, PDI, and MDI in the mixed feeding group within 12-36 months were slightly higher than those in the breastfeeding group and significantly better than those in the artificial feeding group (P < 0.05). The prevalence of the breastfeeding group was 22.2% (43/194) and mixed feeding group was 19.8% (39/199), which was lower than that of the artificial feeding group 57.8% (120/207), P < 0.01. The number of people with normal psychological development was 88.7% (172/194) in the breastfeeding group and 88.9% (177/199) in the mixed feeding group, which was higher than 76.3% (158/207) in the artificial feeding group (P < 0.01). When there is little colostrum secretion in the first 2-3 days of delivery, appropriate addition of formula milk can reduce the incidence of diseases in newborn infants. Breast milk is the most perfect natural food. The immune substance in breast milk is an important element for infants to resist diseases. The communication between mother and child is the first condition for the development of infant mental health. | 2022 | Applied bionics and biomechanics | No DOI | [
{
"LastName": "Xiaoli",
"FirstName": "Shi",
"Affiliation": "Neonatal Care Unit of Pediatric Internal Medicine, Laizhou People's Hospital, Yantai, 261400 Shandong, China."
},
{
"LastName": "Weiyan",
"FirstName": "Hao",
"Affiliation": "Neonatal Care Unit of Pediatric Internal Medicine, Laizhou People's Hospital, Yantai, 261400 Shandong, China."
},
{
"LastName": "Li",
"FirstName": "Dandan",
"Affiliation": "Pharmaceutical Engineering, Pharmaceutical Engineering Part, Jiangsu Ocean University, 222005 Lianyungang, Jiangsu, China."
}
] | Yes |
35192413 | Acting Like a Baby Boomer? Birth-Cohort Differences in Adults' Personality Trajectories During the Last Half a Century. | Society and developmental theory generally assume that there are wide generational differences in personality. Yet evidence showing historical change in the levels of adult Big Five traits is scarce and particularly so for developmental change. We tracked adult trajectories of personality in 4,732 participants (age: M = 52.93 years, SD = 16.69; 53% female) from the Seattle Longitudinal Study (born 1883-1976) across 50 years. Multilevel models revealed evidence for historical change in personality: At age 56, later-born cohorts exhibited lower levels of maturity-related traits (agreeableness and neuroticism) and higher levels of agency-related traits (extraversion and openness) than earlier-born cohorts. Historical changes in agreeableness and neuroticism were more pronounced among young adults, but changes in openness were less pronounced. Cohort differences in change were rare and were observed only for agreeableness; within-person increases were more pronounced among later-born cohorts. Our results yield the first evidence for historical change in the Big Five across adulthood and point to the roles of delayed social-investment and maturity effects. | 2022 Mar | Psychological science | No DOI | [
{
"LastName": "Brandt",
"FirstName": "Naemi D",
"Affiliation": "Department of Psychology, University of Hamburg."
},
{
"LastName": "Drewelies",
"FirstName": "Johanna",
"Affiliation": "Department of Psychology, Humboldt University of Berlin."
},
{
"LastName": "Willis",
"FirstName": "Sherry L",
"Affiliation": "Department of Psychiatry and Behavioral Sciences, University of Washington."
},
{
"LastName": "Schaie",
"FirstName": "K Warner",
"Affiliation": "Department of Psychiatry and Behavioral Sciences, University of Washington."
},
{
"LastName": "Ram",
"FirstName": "Nilam",
"Affiliation": "Department of Communication, Stanford University."
},
{
"LastName": "Gerstorf",
"FirstName": "Denis",
"Affiliation": "Department of Psychology, Humboldt University of Berlin."
},
{
"LastName": "Wagner",
"FirstName": "Jenny",
"Affiliation": "Department of Psychology, University of Hamburg."
}
] | No |
30114636 | Analgesic effects of TLR4/NF-κB signaling pathway inhibition on chronic neuropathic pain in rats following chronic constriction injury of the sciatic nerve. | Chronic neuropathic pain (CNP) is attributed to a lesion or disease of the somatosensory system, may be derived from the peripheral and central system. Recent study revealed that spinal cord stimulation attenuated CNP by inhibiting TLR4/NF-κB signaling pathway. The present study focuses on the potential analgesic effects of TLR4/NF-κB signaling pathway on CNP in a rat model of chronic constriction injury (CCI). We successfully established the rat model of CCI by Bennett method, and then inhibited the TLR4/NF-κB signaling pathway in rat models. Next, we measured the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) 0D, 2D, 6D, 8D and 12D after operation respectively. MTS510 100 mg/kg, an inhibitor of TLR4, was intrathecal injected into rats after 6D, 8D and 12D after operation. The experiment lasted for 12 days, and then the rats were sacrificed to collect the spinal cord tissues. Protein and mRNA expression levels of toll-like receptor 4 (TLR4), nuclear factor-kappaB (NF-κB), glial cell line-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP) and nerve growth factor (NGF) were detected by western blot analysis and RT-qPCR, respectively. Immunohistochemistry was performed to detect GDNF, GFAP and NGF expression. With the prolongation of MTS510 treatment time, MWT and TWL were increased and finally, the MWT and TWL were close to the baseline level. The levels of TLR4, NF-κB, GDNF, and GFAP as well as NGF increased in rats treated with CCI + Immunoglobulin G1 (IgG1) or CCI + MTS510, suggesting the model establishment was successful. Besides, with the prolongation of MTS510 treatment time, the protein level and mRNA expression of NF-kB, GDNF, GFAP and NGF decreased in rats treated with CCI + IgG1 or CCI + MTS510. Moreover, the GDNF, GFAP and NGF expression in spinal cord tissue in rats treated with CCI + IgG1 or CCI + MTS510 increased obviously, while the GDNF, GFAP and NGF expression decreased in spinal cord tissue in rats treated with CCI + IgG1 or CCI + MTS510 after MTS510 treatment. Collectively, this study defines the role of TLR4 and NF-κB, and inhibition of TLR4/NF-κB signaling pathway might contribute to the alleviation of CNP and improvement of MWT and TWL in a rat model of CCI. Additionally, the results obtained from the study provided a promising basis that could aid as an experimental basis for the potential treatment of TLR4/NF-κB signaling pathway. | 2018 Nov | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | No DOI | [
{
"LastName": "Xu",
"FirstName": "Longhe",
"Affiliation": "Department of Anesthesiology, Hainan Branch of Chinese PLA General Hospital, Sanya, 572013, PR China."
},
{
"LastName": "Liu",
"FirstName": "Yaobo",
"Affiliation": "Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, 215123, PR China."
},
{
"LastName": "Sun",
"FirstName": "Yuhui",
"Affiliation": "Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, 215123, PR China."
},
{
"LastName": "Li",
"FirstName": "Hao",
"Affiliation": "Department of Anesthesiology, Hainan Branch of Chinese PLA General Hospital, Sanya, 572013, PR China."
},
{
"LastName": "Mi",
"FirstName": "Weidong",
"Affiliation": "Center of Anesthesiology & Operation, Chinese PLA General Hospital, Beijing, 100853, PR China."
},
{
"LastName": "Jiang",
"FirstName": "Yuge",
"Affiliation": "Department of Anesthesiology, Hainan Branch of Chinese PLA General Hospital, Sanya, 572013, PR China. Electronic address: dovejiang@hotmail.com."
}
] | Yes |
31339375 | Synergistic effects of polyaniline and pulsed electromagnetic field to stem cells osteogenic differentiation on polyvinylidene fluoride scaffold. | Repairing the lost or damaged mandible is very difficult and time-consuming, so there is a great hope for tissue engineering to accelerate it. At the present study, electrospinning was applied to fabricate polyvinylidene fluoride (PVDF) and PVDF-polyaniline (PANI) composite scaffolds. In addition, extremely low frequency pulsed electromagnetic field (PEMF) was applied for treating the stem cells derived from dental pulp (DPSCs) when cultured on the nanofibrous scaffolds. Osteoinductive property of the fabricated PVDF, PVDF-PANI scaffold at the presence and absence of the PEMF was investigated by evaluating the common osteogenic differentiation markers in seeded-DPSCs on the scaffold. Results demonstrated that cell attachment, protein adsorption and cells viability were increased when PEMF was applied. In addition, ALP activity, calcium content, osteogenic genes and protein evaluations confirmed that PEMF could significantly increase osteoinductivity of the PVDF while composite with PANI. According to the results, the use of polymers with piezoelectricity and conductivity features plus PEMF exposure has a promising potential to improve the current treatment methods in bone and mandibular defects. | 2019 Dec | Artificial cells, nanomedicine, and biotechnology | No DOI | [
{
"LastName": "Mirzaei",
"FirstName": "Ali",
"Affiliation": "a Cellular and Molecular Research Center, Yasuj University of Medical Sciences , Yasuj , Iran."
},
{
"LastName": "Saburi",
"FirstName": "Ehsan",
"Affiliation": "c Medical Genetics and Molecular Medicine Department, School of Medicine, Mashhad University of Medical Sciences , Mashhad , Iran."
},
{
"LastName": "Enderami",
"FirstName": "Seyed Ehsan",
"Affiliation": "d Immunogenetics Research Center, Department of Medical Biotechnology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran."
},
{
"LastName": "Barati Bagherabad",
"FirstName": "Matineh",
"Affiliation": "g Department of Modern Sciences and Technologies, Faculty of Medicine, Mashhad University of Medical Science , Mashhad , Iran."
},
{
"LastName": "Enderami",
"FirstName": "Seyedeh Elnaz",
"Affiliation": "h Department of Stem Cell and Regenerative Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB) , Tehran , Iran."
},
{
"LastName": "Chokami",
"FirstName": "Mahmood",
"Affiliation": "i Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences , Karaj , Iran."
},
{
"LastName": "Shapouri Moghadam",
"FirstName": "Abbas",
"Affiliation": "j Department of Immunogenetics, Bu-Ali Research Institute, Mashhad University of Medical Sciences , Mashhad , Iran."
},
{
"LastName": "Salarinia",
"FirstName": "Reza",
"Affiliation": "k Student Research Committee, School of Medicine, North Khorasan University of Medical Sciences , Bojnurd , Iran."
},
{
"LastName": "Ardeshirylajimi",
"FirstName": "Abdolreza",
"Affiliation": "l Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences , Tehran , Iran."
},
{
"LastName": "Mansouri",
"FirstName": "Vahid",
"Affiliation": "m Faculty of Paramedical Sciences, Proteomics Research Center, Shahid Beheshti University of Medical Sciences , Tehran , Iran."
},
{
"LastName": "Soleimanifar",
"FirstName": "Fatemeh",
"Affiliation": "i Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences , Karaj , Iran."
}
] | No |
36061357 | Identification of Candidate Therapeutic Target Genes and Profiling of Tumor-Infiltrating Immune Cells in Pancreatic Cancer via Integrated Transcriptomic Analysis. | Pancreatic cancer (PC) has a dismal prognosis despite advancing scientific and technological knowledge. The exploration of novel genes is critical to improving current therapeutic measures. This research is aimed at selecting hub genes that can act as candidate therapeutic target genes and as prognostic biomarkers in PC. Gene expression profiles of datasets GSE101448, GSE15471, and GSE62452 were extracted from the GEO database. The "limma" package was performed to select differentially expressed genes (DEGs) between PC and normal tissue samples in each dataset. Robust rank aggregation (RRA) algorithm was conducted to integrate multiple expression profiles and identify robust DEGs. GO analysis and KEGG analysis were conducted to identify the functional correlation of the DEGs. The CIBERSORT algorithm was conducted to estimate the immune cell composition of each tissue sample. STRING and Cytoscape were used to establish the protein-protein interaction (PPI) network. The cytoHubba plugin in Cytoscape was performed to identify hub genes. Survival analysis based on hub gene expression was performed with clinical information from TCGA database. 566 robust DEGs (338 upregulated genes and 226 downregulated genes) were identified. Tumor tissue had a higher infiltration of resting dendritic cells and tumor-associated macrophages (TAM), including M0, M1, and M2 macrophages, while infiltration levels of B memory cells, plasma cells, T cells CD8, T follicular helper cells, and NK cells in normal tissue were relatively higher. GO terms and KEGG pathway analysis results revealed enrichment in tumor-associated pathways, including the extracellular matrix organization, cell-substrate adhesion cytokine-cytokine receptor interaction, calcium signaling pathway, and glycine, serine, and threonine metabolism, to name a few. Finally, FN1, MSLN, PLAU, and VCAN were selected as hub genes. High expression of FN1, MSLN, PLAU, and VCAN in PC significantly correlated with poor prognosis. Integrated transcriptomic analysis was used to provide new insights into PC pathogenesis. FN1, MSLN, PLAU, and VCAN may be considered as novel biomarkers of PC. | 2022 | Disease markers | No DOI | [
{
"LastName": "Ding",
"FirstName": "Wei",
"Affiliation": "Department of Hepatobiliary & Pancreatic Surgery, Weifang People's Hospital, No. 151 of Guangwen Street, Weifang, 261041 Shandong Province, China."
},
{
"LastName": "Wang",
"FirstName": "Yuxu",
"Affiliation": "Department of Hepatobiliary & Pancreatic Surgery, Weifang People's Hospital, No. 151 of Guangwen Street, Weifang, 261041 Shandong Province, China."
},
{
"LastName": "Ma",
"FirstName": "Yongbiao",
"Affiliation": "Department of Hepatobiliary & Pancreatic Surgery, Weifang People's Hospital, No. 151 of Guangwen Street, Weifang, 261041 Shandong Province, China."
},
{
"LastName": "Lin",
"FirstName": "Li",
"Affiliation": "Department of Hepatobiliary & Pancreatic Surgery, Weifang People's Hospital, No. 151 of Guangwen Street, Weifang, 261041 Shandong Province, China."
},
{
"LastName": "Li",
"FirstName": "Manjiang",
"Affiliation": "Department of Hepatobiliary & Pancreatic Surgery, Weifang People's Hospital, No. 151 of Guangwen Street, Weifang, 261041 Shandong Province, China."
}
] | Yes |
37421585 | Prevalence of Positivity for Diabetes-Associated Autoantibodies in Individuals with Type 2 Diabetes and Their Further Characterisation: Cross-sectional Study from Slovakia. | Individuals initially diagnosed with type 2 diabetes (T2D) might exhibit positivity for diabetes-associated autoantibodies (DAA +). We investigated the prevalence of DAA positivity in a group of individuals with T2D who were referred to a tertiary diabetes centre within a pre-specified period of time. We aimed to identify characteristics linked with DAA positivity by comparing DAA + individuals with their DAA-negative counterparts. This was a cross-sectional study into which all T2D patients referred to the National Institute of Endocrinology and Diabetology, Ľubochňa, Slovakia, between 1 January and 30 June 2016 were included. Data on > 70 participants' characteristics, including antibodies against glutamic acid decarboxylase (anti-GAD65), insulinoma-associated antigen IA-2 (IA-2A) and insulin (IAA), were collected. Six hundred and ninety-two individuals (387, 55.6% female) with a median (range) age of 62 (24-83) years, HbA1c of 8.9 (5.0-15.7)% [74 (31-148 mmol/mol)] and diabetes duration of 13.0 (0-42) years were analysed. One hundred and forty-five (145/692, 21.0%) tested positive for at least one DAA; 136/692 (19.7%) were positive for anti-GAD65, 21/692 (3.0%) were positive for IA-2A and 9/692 (1.3%) were positive for IAA. Only 84.9% of the DAA + individuals aged > 30 years at the time of diabetes diagnosis met the current diagnostic criteria for latent autoimmune diabetes of adults (LADA). DAA + differed from DAA - individuals in multiple characteristics, including the incidence of hypoglycaemia. Several pathological processes linked with distinct types of diabetes can develop in parallel, including insulin resistance and autoimmune insulitis. In this single-centre cross-sectional study from Slovakia, we report a higher than previously published prevalence of DAA positivity in a group of individuals with a formal diagnosis of T2D. | 2023 Sep | Diabetes therapy : research, treatment and education of diabetes and related disorders | No DOI | [
{
"LastName": "Rončáková",
"FirstName": "Mariana",
"Affiliation": "National Institute of Endocrinology and Diabetology, Ľubochňa, Slovakia."
},
{
"LastName": "Davani",
"FirstName": "Arash",
"Affiliation": "National Institute of Endocrinology and Diabetology, Ľubochňa, Slovakia."
},
{
"LastName": "Mikušová",
"FirstName": "Veronika",
"Affiliation": "National Institute of Endocrinology and Diabetology, Ľubochňa, Slovakia."
},
{
"LastName": "Ságová",
"FirstName": "Ivana",
"Affiliation": "National Institute of Endocrinology and Diabetology, Ľubochňa, Slovakia."
},
{
"LastName": "Novodvorský",
"FirstName": "Peter",
"Affiliation": "Diabetes Centre, Institute for Clinical and Experimental Medicine (IKEM), Prague, Czech Republic."
},
{
"LastName": "Martinka",
"FirstName": "Emil",
"Affiliation": "National Institute of Endocrinology and Diabetology, Ľubochňa, Slovakia. martinka@nedu.sk."
}
] | No |
35812721 | The anti-COVID-19 drug Favipiravir: Degradation, Method development, Validation, NMR/LC-MS characterization, and In-vitro safety evaluation. | It is critical to characterize the degradation products of therapeutic drugs to determine their safety as these degradation products may possess fatal effects on the human physiological system. Favipiravir (FVP), a novel anti-Covid-19 drug, that is recently used all over the world with a great impact on humanity was our target to explore more about its toxicity, the margins of its safety, and its degradants in different degradation conditions. The goal of this study is to identify, characterize, and confirm the structures of FVP oxidative and alkaline breakdown products, as well as to assess their safety utilizing in-vitro SRB cytotoxicity assay on normal human skin fibroblasts (NHSF) cell lines. After oxidative and alkaline degradation of FVP, one degradation product was produced in each condition which was isolated from FVP using flash chromatography, characterized by [1]HNMR and LC-MS/MS techniques. A reversed-phase Thermo Fischer Hypersil C18 column (4.6 × 150 mm, 5 m) was used to achieve HPLC chromatographic separation. Acetonitrile-5 mM potassium dihydrogen phosphate (pH 2.5) (50:50, v/v) was employed as the mobile phase, with a flow rate of 1 mL/min. At 332 nm, the column effluent was measured. Over the concentration range of 0.5-100 µg/mL, the calibration curve was linear. The intra-day and inter-day relative standard deviations were less than 2%, and good percentage recoveries were obtained that fulfilled the acceptance criteria of the International Conference on Harmonization (ICH) recommendations. The Plackett-Burman design was used to assess the robustness. Each degradant was isolated single using Flash chromatography and methylene chloride: methanol gradient mobile phase. The chemical structures of the degradation products have been confirmed and compared to the intact FVP using [1]H-NMR, and Mass spectroscopy. A postulated mechanism of the degradation process has been depicted and the degradants fragmentation pattern has been portrayed. In addition, the in vitro SRB cytotoxicity assay to evaluate the safety profile of FVP and the degradation end products showed their high safety margin in both conditions with IC50 ˃100 µg/ml with no signs of toxicity upon examination of the treated NHSF cells under the optical microscope. | 2022 | Chemicke zvesti | No DOI | [
{
"LastName": "Abdallah",
"FirstName": "Inas A",
"Affiliation": "Department of Analytical Chemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, 32897 Egypt."
},
{
"LastName": "El-Behairy",
"FirstName": "Mohammed F",
"Affiliation": "Department of Organic and Medicinal Chemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, 32897 Egypt."
},
{
"LastName": "Ahmed",
"FirstName": "Rasha M",
"Affiliation": "Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Misr International University, Cairo, Egypt."
},
{
"LastName": "Fayed",
"FirstName": "Marwa A A",
"Affiliation": "Department of Pharmacognosy, Faculty of Pharmacy, University of Sadat City, Sadat City, 32897 Egypt."
}
] | No |
28163799 | Psychological well-being among religious and spiritual-identified young gay and bisexual men. | Religiosity and spirituality are often integral facets of human development. Young gay and bisexual men (YGBM), however, may find themselves at odds when attempting to reconcile potentially conflicting identities like religion and their sexual orientation. We sought to explore how different components of religiosity (participation, commitment, spiritual coping) are linked to different markers of psychological well-being (life purpose, self-esteem, and internalized homophobia). Using data collected in Metro Detroit (N = 351 ages 18-29 years; 47% African American, 29% Non-Latino White, 8% Latino, 16% Other Race), we examined how components of religiosity/spirituality were associated with psychological well-being among religious/spiritual-identified participants. An overwhelming majority (79.5%) identified as religious/spiritual, with most YGBM (91.0%) reporting spirituality as a coping source. Over three quarters of our religious/spiritual sample (77.7%) reported attending a religious service in the past year. Religious participation and commitment were negatively associated with psychological well-being. Conversely, spiritual coping was positively associated with YGBM's psychological well-being. Programs assisting YGBM navigate multiple/conflicting identities through sexuality-affirming resources may aid improve of their psychological well-being. We discuss the public health potential of increasing sensitivity to the religious/spiritual needs of YGBM across social service organizations. | 2016 Mar | Sexuality research & social policy : journal of NSRC : SR & SP | No DOI | [
{
"LastName": "Meanley",
"FirstName": "Steven",
"Affiliation": "The Center for Sexuality & Health Disparities, University of Michigan School of Public Health, Ann Arbor, MI."
},
{
"LastName": "Pingel",
"FirstName": "Emily S",
"Affiliation": "The Center for Sexuality & Health Disparities, University of Michigan School of Public Health, Ann Arbor, MI."
},
{
"LastName": "Bauermeister",
"FirstName": "José A",
"Affiliation": "The Center for Sexuality & Health Disparities, University of Michigan School of Public Health, Ann Arbor, MI."
}
] | No |
34938353 | Analysis of Anesthesia Effect of Dexmedetomidine in Clinical Operation of Replantation of Severed Finger. | Brachial plexus block commonly used in finger replantation has the advantages of simple operation, small side effects, and stable circulation, but it has inherent problems such as imperfect block range, slow onset of anesthesia, and short maintenance time of anesthesia. In order to explore the reliable clinical anesthesia effect, this paper uses experimental investigation methods to study the effect of dexmedetomidine in clinical surgery of replantation of severed fingers. Moreover, this paper uses comparative test methods, uses statistical methods to process test data, and uses intuitive methods to display test results. Finally, this paper verifies the reliability of dexmedetomidine in replantation of severed finger through comparative analysis and verifies that the anesthesia method proposed in this paper has certain user satisfaction through parameter survey. | 2021 | Computational and mathematical methods in medicine | No DOI | [
{
"LastName": "Xu",
"FirstName": "Tongsheng",
"Affiliation": "Wuxi 9th People's Hospital, Wuxi, China."
},
{
"LastName": "Chen",
"FirstName": "Xiaodong",
"Affiliation": "Wuxi 9th People's Hospital, Wuxi, China."
},
{
"LastName": "Li",
"FirstName": "Xin",
"Affiliation": "Wuxi 9th People's Hospital, Wuxi, China."
},
{
"LastName": "Wang",
"FirstName": "Mingzhu",
"Affiliation": "Wuxi 9th People's Hospital, Wuxi, China."
},
{
"LastName": "Wang",
"FirstName": "Meng",
"Affiliation": "Wuxi 9th People's Hospital, Wuxi, China."
}
] | Yes |
15030484 | Induction of endothelial apoptosis by 4-hydroxyhexenal. | Lipid peroxidation and its products such as 4-hydroxy-2-nonenal (HNE) and 4-hydroxyhexenal (HHE) are known to affect redox balance during aging and various degenerative processes, including vascular dysfunction. Deterioration of the endothelial cells that line the vascular wall is known to be an underlying cause of vascular dysfunction. At present, little is known about the mechanism by which HHE induces endothelial cell death (i.e. apoptosis), although HNE-induced apoptotic cell death has been reported. The aim of this study was to determine whether apoptosis induced by HHE in endothelial cells involves peroxynitrite (ONOO(-)). Our results show that in endothelial cells HHE triggers apoptotic cell death by inducing apoptotic Bax coupled with a decrease in anti-apoptotic Bcl-2. Results show that HHE induces reactive oxygen species (ROS), nitric oxide, and ONOO(-) generation, leading to redox imbalance. Furthermore, the antioxidant N-acetyl cysteine, ROS scavenger, and penicillamine, an ONOO(-) scavenger, were found to block HHE-mediated apoptosis. We used confocal laser microscopy to estimate the ability of these inhibitors to attenuate HHE-induced intracellular ONOO(-) levels thus confirming the oxidative mediation of apoptosis in endothelial cells. These findings strongly suggest that accumulated HHE triggers reactive species-mediated endothelial apoptosis, leading to vascular dysfunction as well as vascular aging. During aging, increased lipid peroxidation and its associated production of HHE may exacerbate the weakened redox balance, leading to various chronic degenerative processes including vascular dysfunction. | 2004 Apr | European journal of biochemistry | No DOI | [
{
"LastName": "Lee",
"FirstName": "Ji Young",
"Affiliation": "Genetic Engineering Research Institute, Pusan National University, Busan, Korea."
},
{
"LastName": "Je",
"FirstName": "Jeong Hwan",
"Affiliation": ""
},
{
"LastName": "Kim",
"FirstName": "Dae Hyun",
"Affiliation": ""
},
{
"LastName": "Chung",
"FirstName": "Sang Woon",
"Affiliation": ""
},
{
"LastName": "Zou",
"FirstName": "Yani",
"Affiliation": ""
},
{
"LastName": "Kim",
"FirstName": "Nam Deuk",
"Affiliation": ""
},
{
"LastName": "Ae Yoo",
"FirstName": "Mie",
"Affiliation": ""
},
{
"LastName": "Suck Baik",
"FirstName": "Hyung",
"Affiliation": ""
},
{
"LastName": "Yu",
"FirstName": "Byung Pal",
"Affiliation": ""
},
{
"LastName": "Chung",
"FirstName": "Hae Young",
"Affiliation": ""
}
] | No |
39038016 | Immunohistochemistry-based investigation of MYC, BCL2, and Ki-67 protein expression and their clinical impact in diffuse large B-cell lymphoma in upper Northern Thailand. | Diffuse large B-cell lymphoma (DLBCL) is an aggressive type of non-Hodgkin lymphoma (NHL) that accounts for approximately 25-40% of all NHL cases. The objective of this study was to investigate the protein expression, clinical impact, and prognostic role of MYC, BCL2, and Ki-67 in Thai DLBCL patients. A retrospective analysis was conducted on 100 DLBCL patients diagnosed between January 2018 and December 2019. Immunohistochemistry was used to assess the expression of MYC, BCL2, and Ki-67. The study revealed a significant association between extranodal involvement and positive cases of MYC and BCL2. MYC expressions were associated with Ki-67 expression, while BCL2 positivity was associated with the non-germinal center B-cell (non-GCB) subtype. However, there were no significant differences in the three-year overall survival (OS) and three-year progression-free survival (PFS) rates when using cut-off points of ≥ 40% for MYC, ≥ 50% for BCL2, and ≥ 70% for Ki-67. Notably, DLBCL cases with co-expression of MYC and BCL2 exhibited significantly inferior three-year OS compared to other cases (0% vs. 53%; p = 0.020). Multivariate analysis identified age ≥ 60 years and Eastern Cooperative Oncology Group (ECOG) performance status as independent prognostic factors. In conclusion, MYC, BCL2, and Ki-67 expression can serve as prognostic biomarkers; however, their prognostic value may vary based on the specific cut-off values used. Therefore, determining the appropriate threshold for each biomarker based on individual laboratory analyses and clinical outcomes is crucial. | 2024 | PloS one | No DOI | [
{
"LastName": "Yimpak",
"FirstName": "Phuttirak",
"Affiliation": "Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."
},
{
"LastName": "Bumroongkit",
"FirstName": "Kanokkan",
"Affiliation": "Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."
},
{
"LastName": "Tantiworawit",
"FirstName": "Adisak",
"Affiliation": "Department of Internal Medicine, Division of Hematology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."
},
{
"LastName": "Rattanathammethee",
"FirstName": "Thanawat",
"Affiliation": "Department of Internal Medicine, Division of Hematology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."
},
{
"LastName": "Aungsuchawan",
"FirstName": "Sirinda",
"Affiliation": "Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."
},
{
"LastName": "Daroontum",
"FirstName": "Teerada",
"Affiliation": "Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."
}
] | No |
33576589 | New catalogue of conditions that may require pediatric palliative care. | Pediatric palliative care applies to the conditions specified in the Regulation of the Minister of Health. The previous studies have proved that the conditions' catalogue is incomplete and inadequate. The aim of the study was to verify completeness and adequacy of the Minister of Health's catalogue and to develop a new catalogue based on available epidemiological data concerning the Polish population. The data of the Statistics Poland on underlying causes of death of children (0-17 years old) and young adults (18-29 years old) who died in 1999-2018 were analyzed. Besides, the study includes data collected by the Warsaw Hospice for Children Foundation on diagnoses of 739 patients treated in 1994-2019, including 519 deceased. The catalogue developed on that basis was compared with the Minister of Health's catalogue. The new catalogue of conditions that may require pediatric palliative care in Poland and the additional criteria of patient recruitment were developed. The incompleteness and inadequacy of the Minister of Health's catalogue were demonstrated. The Regulation of the Minister of Health necessitates an amendment regarding the list of medical conditions entitling children to guaranteed palliative care and hospice services. The Minister of Health should introduce standards and medical procedures for pediatric palliative care. Patients should be recruited on the basis of recognized standards by physicians and not officials of the National Health Fund using incomplete and inadequate Minister of Health's catalogue of medical conditions. | 2020 | Przeglad epidemiologiczny | No DOI | [
{
"LastName": "Dangel",
"FirstName": "Tomasz",
"Affiliation": "The Warsaw Hospice for Children Foundation, Tadeusz Dąbrowski Research and Training Cente."
},
{
"LastName": "Wojciechowska",
"FirstName": "Urszula",
"Affiliation": "National Cancer Registry, Maria Sklodowska-Curie National Research Institute of Oncology."
}
] | No |
23320932 | Hormonal contraception and HIV acquisition - what is the evidence? What are the policy and operational implications? | Family planning (FP) is essential in achieving the United Nations Millennium Development Goals. We critically review the evidence on HIV acquisition among women using hormonal contraception, and discuss the policy and operational implications. Longitudinal studies conducted in sub-Saharan Africa published between 2008 and 2012, as well as key policy documents related to contraception and HIV were reviewed. Findings on hormonal contraception and HIV acquisition conducted in sub- Saharan Africa are inconsistent. While in the large scale studies no statistically significant association between oral contraceptive use and HIV acquisition was found, results for injectables were mixed. Potential biases, such as those resulting from self-selection, related to the observational study design and main confounders such as condom use, sexual activity and contraceptive use are discussed. It is currently not possible to conclude whether the use of hormonal contraceptives is associated with a greater risk of acquiring HIV, or not. The use of male or female condoms for dual protection should be promoted in FP programmes. While there is need for further research on a broader range of contraceptive methods and HIV transmission, studies documenting acceptability of currently less used/more recent contraceptive methods are also warranted. | 2013 Feb | The European journal of contraception & reproductive health care : the official journal of the European Society of Contraception | No DOI | [
{
"LastName": "Delvaux",
"FirstName": "Thérèse",
"Affiliation": "Department of Public Health, Institute of Tropical Medicine, Antwerp, Belgium. tdelvaux@itg.be"
},
{
"LastName": "Buvé",
"FirstName": "Anne",
"Affiliation": ""
}
] | No |
35965630 | Radiation Exposure of Crystalline Bodies during Interventional Therapy and Related Research Status. | The aim of this study was to estimate the exposure dose level of ocular corpuscles during the common interventional procedures of interventional radiology workers. This article retrospectively analyzed the general instruments and equipment of interventional therapy, the general methods and steps of interventional therapy, and the influence of X-ray of interventional surgery. The related contents of eye protection for interventional radiology workers were summarized. This study found that there was a significant linear correlation between the equivalent dose of lens and the exposure time. The tube voltage, tube current, and the size of fluoroscopic field of vision also have an impact on the radiation of workers during surgical exposure. Interventional surgery is difficult and easy. The exposure time of difficult surgery is relatively long, so the dose received by interventional staff is large. The intervention staff should improve their professional knowledge, be proficient in the operation technology, and try to reduce the exposure without affecting the diagnosis and treatment effect. During the intervention operation, the eye substance of the operator is exposed to a certain dose of radiation, and the wearing rate of protective lead glasses of the intervention staff is very low. Therefore, we must pay attention to the protection of the eyes, strengthen the protection management, and reduce the exposure dose of the intervention staff as much as possible. | 2022 | Contrast media & molecular imaging | No DOI | [
{
"LastName": "Chen",
"FirstName": "Yahong",
"Affiliation": "Catheter Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130031, China."
},
{
"LastName": "Zhao",
"FirstName": "Dong",
"Affiliation": "Catheter Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130031, China."
},
{
"LastName": "Zhong",
"FirstName": "Hongwei",
"Affiliation": "Catheter Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130031, China."
},
{
"LastName": "Zhang",
"FirstName": "Yiheng",
"Affiliation": "Catheter Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130031, China."
},
{
"LastName": "An",
"FirstName": "Donghong",
"Affiliation": "Catheter Room, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130031, China."
}
] | Yes |
34785905 | SNHG8 is Upregulated in Esophageal Squamous Cell Carcinoma and Directly Sponges microRNA-411 to Increase Oncogenicity by Upregulating KPNA2 [Retraction]. | [This retracts the article DOI: 10.2147/OTT.S214881.]. | 2021 | OncoTargets and therapy | No DOI | [] | No |
36713878 | Electrolyte imbalances and dehydration play a key role in Batrachochytrium salamandrivorans chytridiomycosis. | One of the most important emerging infectious diseases of amphibians is caused by the fungal pathogen Batrachochytrium salamandrivorans (Bsal). Bsal was recently discovered and is of global concern due to its potential to cause high mortality in amphibians, especially salamander species. To date, little has been reported on the pathophysiological effects of Bsal; however, studies of a similar fungus, B. dendrobatidis (Bd), have shown that electrolyte losses and immunosuppression likely play a key role in morbidity and mortality associated with this disease. The goal of this study was to investigate pathophysiological effects and immune responses associated with Bsal chytridiomycosis using 49 rough-skinned newts (Taricha granulosa) as the model species. Taricha granulosa were exposed to a 1 × 107 per 10 mL dose of Bsal zoospores and allowed to reach various stages of disease progression before being humanely euthanized. At the time of euthanasia, blood was collected for biochemical and hematological analyses as well as protein electrophoresis. Ten standardized body sections were histologically examined, and Bsal-induced skin lesions were counted and graded on a scale of 1-5 based on severity. Results indicated that electrolyte imbalances and dehydration induced by damage to the epidermis likely play a major role in the pathogenesis of Bsal chytridiomycosis in this species. Additionally, Bsal-infected, clinically diseased T. granulosa exhibited a systemic inflammatory response identified through alterations in complete blood counts and protein electrophoretograms. Overall, these results provide foundational information on the pathogenesis of this disease and highlight the differences and similarities between Bsal and Bd chytridiomycosis. | 2022 | Frontiers in veterinary science | No DOI | [
{
"LastName": "Sheley",
"FirstName": "Wesley C",
"Affiliation": "Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, Knoxville, TN, United States."
},
{
"LastName": "Gray",
"FirstName": "Matthew J",
"Affiliation": "Center for Wildlife Health, University of Tennessee Institute of Agriculture, Knoxville, TN, United States."
},
{
"LastName": "Wilber",
"FirstName": "Mark Q",
"Affiliation": "Center for Wildlife Health, University of Tennessee Institute of Agriculture, Knoxville, TN, United States."
},
{
"LastName": "Cray",
"FirstName": "Carolyn",
"Affiliation": "Division of Comparative Pathology, University of Miami Miller School of Medicine, Miami, FL, United States."
},
{
"LastName": "Carter",
"FirstName": "E Davis",
"Affiliation": "Center for Wildlife Health, University of Tennessee Institute of Agriculture, Knoxville, TN, United States."
},
{
"LastName": "Miller",
"FirstName": "Debra L",
"Affiliation": "Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, Knoxville, TN, United States."
}
] | No |
33575214 | MRD Tailored Therapy in AML: What We Have Learned So Far. | Acute myeloid leukemia (AML) is a heterogeneous clonal disease associated with a dismal survival, partly due to the frequent occurrence of relapse. Many patient- and leukemia-specific characteristics, such as age, cytogenetics, mutations, and measurable residual disease (MRD) after intensive chemotherapy, have shown to be valuable prognostic factors. MRD has become a rich field of research where many advances have been made regarding technical, biological, and clinical aspects, which will be the topic of this review. Since many laboratories involved in AML diagnostics have experience in immunophenotyping, multiparameter flow cytometry (MFC) based MRD is currently the most commonly used method. Although molecular, quantitative PCR based techniques may be more sensitive, their disadvantage is that they can only be applied in a subset of patients harboring the genetic aberration. Next-generation sequencing can assess and quantify mutations in many genes but currently does not offer highly sensitive MRD measurements on a routine basis. In order to provide reliable MRD results, MRD assay optimization and standardization is essential. Different techniques for MRD assessment are being evaluated, and combinations of the methods have shown promising results for improving its prognostic value. In this regard, the load of leukemic stem cells (LSC) has also been shown to add to the prognostic value of MFC-MRD. At this moment, MRD after intensive chemotherapy is most often used as a prognostic factor to help stratify patients, but also to select the most appropriate consolidation therapy. For example, to guide post-remission treatment for intermediate-risk patients where MRD positive patients receive allogeneic stem cell transplantation and MRD negative receive autologous stem cell transplantation. Other upcoming uses of MRD that are being investigated include: selecting the type of allogeneic stem cell transplantation therapy (donor, conditioning), monitoring after stem cell transplantation (to allow intervention), and determining drug efficacy for the use of a surrogate endpoint in clinical trials. | 2020 | Frontiers in oncology | No DOI | [
{
"LastName": "Ngai",
"FirstName": "Lok Lam",
"Affiliation": "Department of Hematology, Amsterdam UMC, Cancer Center Amsterdam, Vrije Universiteit, Amsterdam, Netherlands."
},
{
"LastName": "Kelder",
"FirstName": "Angèle",
"Affiliation": "Department of Hematology, Amsterdam UMC, Cancer Center Amsterdam, Vrije Universiteit, Amsterdam, Netherlands."
},
{
"LastName": "Janssen",
"FirstName": "Jeroen J W M",
"Affiliation": "Department of Hematology, Amsterdam UMC, Cancer Center Amsterdam, Vrije Universiteit, Amsterdam, Netherlands."
},
{
"LastName": "Ossenkoppele",
"FirstName": "Gert J",
"Affiliation": "Department of Hematology, Amsterdam UMC, Cancer Center Amsterdam, Vrije Universiteit, Amsterdam, Netherlands."
},
{
"LastName": "Cloos",
"FirstName": "Jacqueline",
"Affiliation": "Department of Hematology, Amsterdam UMC, Cancer Center Amsterdam, Vrije Universiteit, Amsterdam, Netherlands."
}
] | No |
27532124 | Prognostic Role of Secretory Clusterin in Multiple Human Malignant Neoplasms: A Meta-Analysis of 26 Immunohistochemistry Studies. | Secretory clusterin (sCLU) is a potential prognostic tumour biomarker, but results of different sCLU studies are inconsistent. We conducted this meta-analysis to evaluate the precise predictive value of sCLU. Qualified studies were identified by performing online searches in PubMed, EMBASE, and Web of Science. The selected articles were divided into three groups based on scoring method for clusterin detection. Pooled hazard ratios (HRs) with 95% confidence interval (CI) for patient survival and disease recurrence were calculated to determine the correlation between sCLU expression and cancer prognosis. Heterogeneity was assessed using I2 statistics, and specific heterogeneity in different groups was analysed. Elevated sCLU was significantly associated with recurrence-free survival in groups 1 and 3 (group 1: pooled HR = 1.35, 95% CI = 1.01 to 1.79; group 3: pooled HR = 1.80, 95% CI = 1.22 to 2.65). However, clusterin expression was not associated with overall survival in all three groups. Results showed that only the heterogeneity of group 2 was very strong (p = 0.013, I2 = 76.3%), in which the specimens were scored through sCLU staining intensity only. sCLU is a potential biomarker for tumour prognosis, and IHC methods can be more standardised if both intensity and staining proportion are considered. | 2016 | PloS one | No DOI | [
{
"LastName": "Zhang",
"FirstName": "Jianzhong",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Miao",
"FirstName": "Chenkui",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Xu",
"FirstName": "Aiming",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Zhao",
"FirstName": "Kai",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Qin",
"FirstName": "Zhiqiang",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Li",
"FirstName": "Xiao",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Liang",
"FirstName": "Chao",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Hua",
"FirstName": "Yibo",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Chen",
"FirstName": "Wei",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Zhang",
"FirstName": "Chao",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Liu",
"FirstName": "Yiyang",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Su",
"FirstName": "Shifeng",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Wang",
"FirstName": "Zengjun",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
},
{
"LastName": "Liu",
"FirstName": "Bianjiang",
"Affiliation": "State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing. 210029, China."
}
] | Yes |
34427689 | [Rare superinfection in a COVID-19 patient-A chronology]. | After a resuscitation situation a SARS-CoV‑2 sample from a 55-year-old man who had been in the hospital for elective ablation for atrial fibrillation was tested positive. The patient history revealed that there had been a previous confirmed contact with a COVID-19 positive patient. The patient developed the complete set of symptoms of COVID-19 pneumonia with extensive intensive care treatment. After about 2 weeks of treatment, weaning had to be stopped due to the deterioration of the severe septic condition of the patient and he showed microbiological evidence of a superinfection with Cryptococcus neoformans and later Leclercia adecarboxylata. The patient was treated successfully and survived the disease. | 2022 Jan | Der Anaesthesist | No DOI | [
{
"LastName": "Gamon",
"FirstName": "E",
"Affiliation": "Klinik für Anästhesiologie, Intensivmedizin und Notfallmedizin, Klinikum Links der Weser, Bremen, Deutschland. e.gamon@mail.de."
},
{
"LastName": "Tammena",
"FirstName": "D",
"Affiliation": "Klinik für Anästhesiologie, Intensivmedizin und Notfallmedizin, Klinikum Links der Weser, Bremen, Deutschland."
},
{
"LastName": "Wattenberg",
"FirstName": "M",
"Affiliation": "Klinik für Anästhesiologie, Intensivmedizin und Notfallmedizin, Klinikum Links der Weser, Bremen, Deutschland."
},
{
"LastName": "Augenstein",
"FirstName": "T",
"Affiliation": "Klinik für Anästhesiologie, Intensivmedizin und Notfallmedizin, Klinikum Links der Weser, Bremen, Deutschland."
}
] | No |
38616090 | Nanocellulose-mediated bilayer hydrogel actuators with thermo-responsive, shape memory and self-sensing performances. | Inspired by creatures, abundant stimulus-responsive hydrogel actuators with diverse functionalities have been manufactured for applications in soft robotics. However, constructing a shape memory and self-sensing bilayer hydrogel actuator with high mechanical strength and strong interfacial bonding still remains a challenge. Herein, a novel bilayer hydrogel with a stimulus-responsive TEMPO-oxidized cellulose nanofibers/poly(N-isopropylacrylamide) (TOCN/PNIPAM) layer and a non-responsive TOCN/polyacrylamide (TOCN/PAM) layer is proposed as a thermosensitive actuator. TOCNs as a nano-reinforced phase provide a high mechanical strength and endow the hydrogel actuator with a strong interfacial bonding. Due to the incorporation of TOCNs, the TOCN/PNIPAM hydrogel exhibits a high compressive strength (~89.2 kPa), elongation at break (~170.7 %) and tensile strength (~24.0 kPa). The prepared PNIPAM/TOCN/PAM hydrogel actuator performs the roles of an encapsulation, jack, temperature-controlled fluid valve and temperature-control manipulator. The incorporation of Fe[3+] further endows the bilayer hydrogel actuator with a synergistic performance of shape memory and temperature-driven, which can be used as a temperature-responsive switch to detect ambient temperature. The PNIPAM/TOCN/PAM-Fe[3+] conductive hydrogel can be assembled into a flexible sensor and generate sensing signals when driven by temperature changes to achieve real-time feedback. This research may lead to new insights into the design and manufacturing of intelligent flexible soft robots. | 2024 Jul 1 | Carbohydrate polymers | No DOI | [
{
"LastName": "Ma",
"FirstName": "Yuanyuan",
"Affiliation": "Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, Joint International Research Lab of Lignocellulosic Functional Materials, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China."
},
{
"LastName": "Lu",
"FirstName": "Ya",
"Affiliation": "Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, Joint International Research Lab of Lignocellulosic Functional Materials, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China."
},
{
"LastName": "Yue",
"FirstName": "Yiying",
"Affiliation": "College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China. Electronic address: yue@njfu.edu.cn."
},
{
"LastName": "He",
"FirstName": "Shuijian",
"Affiliation": "Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, Joint International Research Lab of Lignocellulosic Functional Materials, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China."
},
{
"LastName": "Jiang",
"FirstName": "Shaohua",
"Affiliation": "Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, Joint International Research Lab of Lignocellulosic Functional Materials, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China."
},
{
"LastName": "Mei",
"FirstName": "Changtong",
"Affiliation": "College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China."
},
{
"LastName": "Xu",
"FirstName": "Xinwu",
"Affiliation": "College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China."
},
{
"LastName": "Wu",
"FirstName": "Qinglin",
"Affiliation": "School of Renewable Natural Resources, Louisiana State University, Baton Rouge, LA 70803, United States."
},
{
"LastName": "Xiao",
"FirstName": "Huining",
"Affiliation": "Department of Chemical Engineering, University of New Brunswick, 15 Dineen Drive, Fredericton, NB E3B 5A3, Canada."
},
{
"LastName": "Han",
"FirstName": "Jingquan",
"Affiliation": "Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, Joint International Research Lab of Lignocellulosic Functional Materials, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China. Electronic address: hjq@njfu.edu.cn."
}
] | No |
34582105 | Evaluation of atezolizumab immunogenicity: Efficacy and safety (Part 2). | Antibody therapeutics can be associated with unwanted immune responses resulting in the development of anti-drug antibodies (ADA). Optimal methods to evaluate the potential effects of ADA on clinical outcomes in oncology are not well established. In this study, we assessed efficacy and safety, based on ADA status, in patients from over 10 clinical trials that evaluated the immune checkpoint inhibitor atezolizumab as a single agent or as combination therapy for several types of advanced cancers. ADA can only be observed post randomization, and imbalances in baseline prognostic factors can confound the interpretation of ADA impact. We applied methodology to account for the confounding effects of baseline clinical characteristics and survivorship bias on efficacy. Adjusted meta-analyses revealed that despite numerical differences in overall survival and progression-free survival between ADA-positive and ADA-negative patients from some studies, ADA-positive patients from studies with an overall treatment effect derived benefit from atezolizumab, compared with their adjusted controls. Based on large, pooled populations from atezolizumab monotherapy or combination studies, unadjusted descriptive analyses did not identify a clear relationship between ADA status and frequency or severity of adverse events. Data also suggested that any ADA impact is not driven by neutralizing activity. Collectively, this exploratory analysis suggests that the potential for ADA development should not impact treatment decisions with atezolizumab. | 2022 Jan | Clinical and translational science | No DOI | [
{
"LastName": "Peters",
"FirstName": "Solange",
"Affiliation": "Oncology Department - CHUV, Lausanne University, Lausanne, Switzerland."
},
{
"LastName": "Galle",
"FirstName": "Peter R",
"Affiliation": "Department of Internal Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany."
},
{
"LastName": "Bernaards",
"FirstName": "Coen A",
"Affiliation": "Product Development Biometrics, Genentech, Inc, South San Francisco, California, USA."
},
{
"LastName": "Ballinger",
"FirstName": "Marcus",
"Affiliation": "Product Development Oncology, Genentech, Inc, South San Francisco, California, USA."
},
{
"LastName": "Bruno",
"FirstName": "René",
"Affiliation": "Clinical Pharmacology, Genentech-Roche, Marseille, France."
},
{
"LastName": "Quarmby",
"FirstName": "Valerie",
"Affiliation": "BioAnalytical Sciences, Genentech Inc, South San Francisco, California, USA."
},
{
"LastName": "Ruppel",
"FirstName": "Jane",
"Affiliation": "BioAnalytical Sciences, Genentech Inc, South San Francisco, California, USA."
},
{
"LastName": "Vilimovskij",
"FirstName": "Alexandr",
"Affiliation": "Product Development Safety, F Hoffmann-La Roche Ltd, Basel, Switzerland."
},
{
"LastName": "Wu",
"FirstName": "Benjamin",
"Affiliation": "Department of Clinical Pharmacology, Genentech Inc, South San Francisco, California, USA."
},
{
"LastName": "Sternheim",
"FirstName": "Nitzan",
"Affiliation": "Product Development Regulatory, Genentech Inc, South San Francisco, California, USA."
},
{
"LastName": "Reck",
"FirstName": "Martin",
"Affiliation": "Lung Clinic Grosshansdorf, Airway Research Center North, German Center for Lung Research, Grosshansdorf, Germany."
}
] | No |
30622470 | Identification of Pharmacokinetic Markers for Guanxin Danshen Drop Pills in Rats by Combination of Pharmacokinetics, Systems Pharmacology, and Pharmacodynamic Assays. | This paper reported a feasibility study strategy of identifying pharmacokinetic (PK) markers for a cardiovascular herbal medicine, Guanxin Danshen drop pill (GDDP). First, quantification analysis revealed the constituent composition in the preparation by high-performance liquid chromatography (HPLC). Subsequently, physiochemical property calculation predicted the solubility and intestinal permeability of the constituents in the preparation. Furthermore, HPLC-MS analysis ascertained the absorbable ingredients and their PK properties in rat plasma. The main effective substances from the ingredients absorbed into blood and their cardiovascular effects were also predicted by systems pharmacology study, and were further confirmed by in vivo protective effects on isoprenaline-induced myocardial injury in mice. Finally, the ingredients with high content, representative structure feature, favorable PK properties, high relevant degree to myocardial ischemia (MI) issues, and validated therapeutic effects were considered as the PK markers for the preparation. Ginsenosides Rg1, Rb1, and tanshinone (TS) IIA were identified originally as PK markers for representing PK properties of GDDP. In addition, integrated PK studies were carried out according to previous reports, viz. drug concentration sum method and the AUC weighting method, to understand the in vivo process of GDDP comprehensively. The present study maybe provide a reference approach to identify PK markers for cardiovascular herbal medicines. | 2018 | Frontiers in pharmacology | No DOI | [
{
"LastName": "Yao",
"FirstName": "Hong",
"Affiliation": "Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, China."
},
{
"LastName": "Huang",
"FirstName": "Xiaomei",
"Affiliation": "Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, China."
},
{
"LastName": "Xie",
"FirstName": "Yunjiao",
"Affiliation": "Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, China."
},
{
"LastName": "Huang",
"FirstName": "Xuliang",
"Affiliation": "Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, China."
},
{
"LastName": "Ruan",
"FirstName": "Yijun",
"Affiliation": "Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, China."
},
{
"LastName": "Lin",
"FirstName": "Xinhua",
"Affiliation": "Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, China."
},
{
"LastName": "Huang",
"FirstName": "Liying",
"Affiliation": "Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, China."
},
{
"LastName": "Shi",
"FirstName": "Peiying",
"Affiliation": "Department of Traditional Chinese Medicine Resource and Bee Products, Bee Science College, Fujian Agriculture and Forestry University, Fuzhou, China."
}
] | No |
17016400 | Trauma systems. | Trauma is one of the major causes of death and disability in modern society, particularly for the young. Organized trauma systems reduce mortality and morbidity from trauma. An effective trauma system addresses all aspects of trauma care, from prevention to rehabilitation. Well-developed trauma systems are currently available only to a minority of the world's population. Trauma systems in developed nations have much potential for improvement. | 2001 Apr | Current opinion in anaesthesiology | No DOI | [
{
"LastName": "Davis",
"FirstName": "M J",
"Affiliation": "University of New South Wales, Intensive Care Unit, Liverpool Hospital, Liverpool, Sydney, Australia."
},
{
"LastName": "Parr",
"FirstName": "M J",
"Affiliation": ""
}
] | No |
39035471 | Strengths and weaknesses of the South-South Learning Exchange: a qualitative analysis of experts' perspectives. | South-South learning exchange (SSLE) refers to an interactive learning process where peers exchange knowledge and experience to work towards a beneficial change. Despite organizations having recently increased the opportunity to run SSLEs, the SSLE support mechanisms and processes are not well documented in the scientific literature. This study explored experts' perspectives on SSLEs, strengths, weaknesses and mechanisms leading to sustainable outcomes. We conducted a qualitative study using semi-structured interviews on experiences of participants and organizers of SSLEs. Data were collected between 1st September 2021 to 26th November 2021. All data were digitally recorded, transcribed verbatim, and analysed. In the analysis, we adopted an inductive approach derived from thematic analysis. Sixteen experts, who have participated in or facilitated one or more SSLE, were interviewed. The experts' accounts demonstrated an appreciation of participants' empowerment, positive peer-to-peer "mind change" and convincing and powerful hands-on learning of this approach as strengths in the implementation of the SSLE. Being resource heavy, participant and donor reluctance and absence of a validated methodology emerged as main weaknesses of the South-South learning approach, which could impair the effectiveness of this scheme. The strengths of SSLEs are anchored in the theories of experiential and social learning, highlighting SSLE's potential to create an environment that enhances knowledge exchange. the study highlights the challenges SSLE initiatives face. In particular, these include limited commitment and funds, limited evidence of impact, disparate approaches, and the absence of standardized guidelines and evaluation practices. | 2023 | Gates open research | No DOI | [
{
"LastName": "Triulzi",
"FirstName": "Isotta",
"Affiliation": "Institute of Management, Scuola Superiore Sant'Anna, Pisa, 56127, Italy."
},
{
"LastName": "Kabra",
"FirstName": "Rita",
"Affiliation": "Department of Sexual and Reproductive Health and Research, World Health Organization, Geneva, 1211, Switzerland."
},
{
"LastName": "Allagh",
"FirstName": "Komal Preet",
"Affiliation": "Consultant, Department of Sexual and Reproductive Health and Research, World Health Organization, Geneva, 1211, Switzerland."
},
{
"LastName": "Kiarie",
"FirstName": "James",
"Affiliation": "Department of Sexual and Reproductive Health and Research, World Health Organization, Geneva, 1211, Switzerland."
}
] | No |
20053117 | Growth and differentiation of the larval mosquito midgut. | Factors affecting larval growth and nutrition have consequences on adult fecundity. Since the mosquito larval midgut is the primary organ of digestion and nutrient absorption, factors that affect the growth and development of the midgut may have potential consequences on the reproductive potential of the adult. To gain a better understanding of mosquito midgut development the growth and metamorphic remodeling of the Aedes aegypti L. and Culex pipiens L. (Diptera: Culicidae) midguts were investigated. Cytological evidence was obtained suggesting that, in both the anterior and posterior Ae. aegypti larval midgut, diploid regenerative cells give rise to new endoreplicating cells that significantly contribute to the growth and metabolism of the midgut. This hypothesis was supported by BrdU incorporation studies showing that diploid cells, as well as large and small endoreplicating cells, synthesize DNA during the 2(nd), 3(rd) and 4(th) instars. Cytological studies of the Cx. pipiens larval midgut suggest that anterior midgut growth in this species is primarily by cell enlargement. To study metamorphic remodeling of the midgut, DNA synthesis in Ae. aegypti 4(th) instar midguts was followed by using 5-bromo-2-deoxyuridine (BrdU) incorporation. During the 24 hr period after the last larval-larval molt both endoreplicating and diploid cells incorporate BrdU. After the critical weight is achieved, endoreplicating cell BrdU incorporation gradually ceases while diploid cells continue to replicate. The period of maximum diploid cell incorporation correlated with the period of maximum ecdysone titer. | 2009 | Journal of insect science (Online) | No DOI | [
{
"LastName": "Ray",
"FirstName": "Kathryn",
"Affiliation": "Biology Department, Brooklyn College, 2900 Bedford Ave, Brooklyn, NY 11210, USA. Kray@brooklyn.cuny.edu"
},
{
"LastName": "Mercedes",
"FirstName": "Maria",
"Affiliation": ""
},
{
"LastName": "Chan",
"FirstName": "Doris",
"Affiliation": ""
},
{
"LastName": "Choi",
"FirstName": "Chi Yan",
"Affiliation": ""
},
{
"LastName": "Nishiura",
"FirstName": "James T",
"Affiliation": ""
}
] | No |
36452989 | Management of biologics in pregnant, lactating patients with inflammatory bowel disease and the impact on neonatal vaccination: A systematic review of clinical practice guidelines and consensus statements. | The management of biological agents during pregnancy poses challenges as maternal and infant safety must be addressed. This study aims to compare the recommendations of existing guidelines on managing the use of biologics during pregnancy, lactation for patients with inflammatory bowel disease, and the influence on neonatal vaccination. The PubMed, EMBASE, China National Knowledge Infrastructure, Wanfang database, China Science and Technology Journal Database and China Biomedical Database were systematically searched from the inception date to 11 May 2022, to screen all relevant guidelines. Quality assessment was performed using the guideline methodology reporting tool AGREE II. Fourteen guidelines and consensus statements with detailed recommendations were included. All guidance documents cover management comments during pregnancy, and most consider that biologics can be given safely during pregnancy but require suspension at the right time to protect the foetus. However, the roles of vedolizumab and ustekinumab are disputed. Five documents guide lactation and the use of most biologics during lactation is safe, but no guidelines recommend vedolizumab. Six papers provide recommendations for newborns' vaccination, suggesting a delay in infants' live vaccination schedule if their mothers are treated with biologics. Our study concluded that future guidelines could consider incorporating newer, more robust evidence to update recommendations. The development of future guidelines needs to consider the involvement of multidisciplinary experts, adequately report on the evidence retrieval process, and provide strategies for implementation. Besides, more research is needed to explore the use of biologics during pregnancy and lactation in patients with inflammatory bowel disease. | 2022 Dec | Journal of clinical pharmacy and therapeutics | No DOI | [
{
"LastName": "Ding",
"FirstName": "Ning",
"Affiliation": "Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, China."
},
{
"LastName": "Zhao",
"FirstName": "Luqing",
"Affiliation": "Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, China."
},
{
"LastName": "Zhu",
"FirstName": "Lingfei",
"Affiliation": "Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China."
},
{
"LastName": "Sun",
"FirstName": "Weijia",
"Affiliation": "Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China."
},
{
"LastName": "Li",
"FirstName": "Danyan",
"Affiliation": "Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, China."
},
{
"LastName": "Li",
"FirstName": "Jiake",
"Affiliation": "Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China."
},
{
"LastName": "Zhang",
"FirstName": "Jun",
"Affiliation": "Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China."
},
{
"LastName": "Zhang",
"FirstName": "Shengsheng",
"Affiliation": "Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, China."
}
] | No |
36730809 | Expression Analysis of CoHMGS in Cornus officinalis and Subcellular Localization of the Enzyme It Encodes. | Cornus officinalis is a perennial deciduous tree or shrub. Its mature fruits are extracted and used in Traditional Chinese Medicine, called Shanzhuyu. The characteristic active components of C. officinalis include loganin and morroniside, which belong to iridoid glycosides. 3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) is a key enzyme in the cytoplasmic mevalonate pathway providing the precursor molecules isopentenyl pyrophosphate and dimethylallyl pyrophosphate for isoprenoid biosynthesis such as sterols, triterpenes, and their derivatives such as iridoid glycosides. Different concentrations of methyl jasmonate (MeJA) and ethephon (ETH) solutions were sprayed on C. officinalis seedlings, and the effect of hormones on CoHMGS gene expression was detected by real-time fluorescence quantitative PCR. The quantitative real-time PCR results showed that 750 mg/L ETH treatment had the most significant induction effect on CoHMGS gene expression. The HPLC analysis of extracts revealed that the treatment could also significantly increase the content of morroniside and loganin in the leaves of C. officinalis. By use of a CoHMGS-green fluorescent protein (GFP) fusion construct for heterologous expression in tobacco, laser scanning confocal microscopy revealed a cytoplasmic localization. This preliminary study of the CoHMGS gene could prepare the ground for more precisely elucidating the synthesis of secondary metabolite in C. officinalis. | 2023 Feb | DNA and cell biology | No DOI | [
{
"LastName": "Zhang",
"FirstName": "Jiaqi",
"Affiliation": "Agricultural College, Henan University of Science and Technology, Luoyang, China."
},
{
"LastName": "Wang",
"FirstName": "Yaoyao",
"Affiliation": "Agricultural College, Henan University of Science and Technology, Luoyang, China."
},
{
"LastName": "Liu",
"FirstName": "Xiaoran",
"Affiliation": "Agricultural College, Henan University of Science and Technology, Luoyang, China."
},
{
"LastName": "Liu",
"FirstName": "Hao",
"Affiliation": "Agricultural College, Henan University of Science and Technology, Luoyang, China."
},
{
"LastName": "Zhao",
"FirstName": "Xingli",
"Affiliation": "Agricultural College, Henan University of Science and Technology, Luoyang, China."
},
{
"LastName": "Lv",
"FirstName": "Shufang",
"Affiliation": "Agricultural College, Henan University of Science and Technology, Luoyang, China."
},
{
"LastName": "Xu",
"FirstName": "Huawei",
"Affiliation": "Agricultural College, Henan University of Science and Technology, Luoyang, China."
},
{
"LastName": "Zhang",
"FirstName": "Hongxiao",
"Affiliation": "Agricultural College, Henan University of Science and Technology, Luoyang, China."
},
{
"LastName": "Hou",
"FirstName": "Xiaogai",
"Affiliation": "Agricultural College, Henan University of Science and Technology, Luoyang, China."
},
{
"LastName": "Hou",
"FirstName": "Dianyun",
"Affiliation": "Agricultural College, Henan University of Science and Technology, Luoyang, China."
}
] | No |
34510600 | Clinical endometritis with Trueperella pyogenes reduces reproductive performance and milk production in dairy cows. | The aim of this study was to identify the impact of Trueperella pyogenes in cows with clinical endometritis (CE) on reproductive performance and milk production in affected cows. In total, 230 lactating Holstein dairy cows from six commercial dairy herds were sampled once between 28 and 33 days post-partum. Cows included in the present study did not receive antibiotic or anti-inflammatory treatments prior to the experimental period. Clinical endometritis (CE) was characterized as cow with vaginal mucus score = 3 (>50% of purulent vaginal discharge) and >18% polymorphonuclear neutrophilic leukocyte (PMNL). The body condition scores (BCS) and milk production were evaluated at the time of enrolment. The identification of isolated bacteria was carried out through the analysis of MALDI-TOF MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry). According to uterine health, three groups of dairy cows were formed: healthy control cows without T. pyogenes (n = 147), CE cows with T. pyogenes (n = 22) and CE cows without T. pyogenes (n = 61). CE cows with T. pyogenes had lower BCS, milk production and conception at first AI (p < .01) than CE cows without T. pyogenes and control cows. Furthermore, CE cows with T. pyogenes had higher (p < .01) service per pregnancy and had greater (p < .01) days to get pregnant than CE cows without T. pyogenes and control cows. This study demonstrates that CE cows with T. pyogenes had impaired reproductive performance and milk production when compared to cows without CE and CE cows without T. pyogenes. This information can contribute to a strategic treatment in cows affected by clinical endometritis, favouring the rational use of antibiotics on dairy farms. | 2021 Dec | Reproduction in domestic animals = Zuchthygiene | No DOI | [
{
"LastName": "Paiano",
"FirstName": "Renan B",
"Affiliation": "Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil."
},
{
"LastName": "Bonilla",
"FirstName": "Jeannine",
"Affiliation": "Departamento de Engenharia de Alimentos, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, Brazil."
},
{
"LastName": "Moreno",
"FirstName": "Andrea M",
"Affiliation": "Departamento de Medicina Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil."
},
{
"LastName": "Baruselli",
"FirstName": "Pietro S",
"Affiliation": "Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil."
}
] | No |
33638698 | Protective and therapeutic effects of the flavonoid "pinocembrin" in indomethacin-induced acute gastric ulcer in rats: impact of anti-oxidant, anti-inflammatory, and anti-apoptotic mechanisms. | Peptic ulcer including gastric and duodenal ulcers is a common gastro-intestinal disorder worldwide, associated with a significant mortality due to bleeding and perforation. Numerous efforts are being exerted to look for natural drugs that lack the potential side effects but still keep beneficial effects for treatment and/or prevention of gastric ulcer. Pinocembrin (PINO) is a natural flavonoid retaining anti-microbial, anti-oxidant, and anti-inflammatory activities. The present study was conducted to investigate the protective and therapeutic effects of PINO against indomethacin (INDO)-induced gastric ulcer in rats and the possible underlying mechanisms. PINO (25 and 50 mg/kg) promoted mucus secretion, decreased ulcer index, and inhibited histopathological changes induced by INDO. Further investigation of possible mechanisms showed that PINO significantly attenuated INDO-induced oxidative and inflammatory responses in both doses when administrated before or after ulcer induction. PINO downregulated mRNA expression level of p38-mitogen-activated protein kinase (p38-MAPK) which subsequently inhibited NF-κB activation and inflammatory cytokine release including tumor necrosis factor-α (TNF-α) and interleukin-1beta (IL-1β). Additionally, PINO inhibited apoptotic activity which was confirmed by downregulation of caspase-3 transcription. The current results demonstrated the promising therapeutic activity of PINO against INDO-induced gastric ulcer due to-at least partly-its anti-oxidant, anti-inflammatory, and anti-apoptotic effects. | 2021 Jul | Naunyn-Schmiedeberg's archives of pharmacology | No DOI | [
{
"LastName": "El-Demerdash",
"FirstName": "Aya A",
"Affiliation": "Department of Pharmacology and Toxicology, Faculty of Pharmacy, Badr University in Cairo (BUC), Cairo, 11829, Egypt."
},
{
"LastName": "Menze",
"FirstName": "Esther T",
"Affiliation": "Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt."
},
{
"LastName": "Esmat",
"FirstName": "Ahmed",
"Affiliation": "Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt. ahmed.esmat@pharma.asu.edu.eg."
},
{
"LastName": "Tadros",
"FirstName": "Mariane G",
"Affiliation": "Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt."
},
{
"LastName": "Elsherbiny",
"FirstName": "Doaa A",
"Affiliation": "Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt."
}
] | Yes |
26819737 | Effect of switching basal insulin regimen to degludec on quality of life in Japanese patients with type 1 and type 2 diabetes mellitus. | Maintainance of a stable basal insulin level is important for glycemic control in treatment of diabetes mellitus. Recently introduced insulin degludec has the longest duration of action among basal insulin formulations. The purpose of this study was to assess changes in quality of life (QOL) associated with switching the basal insulin regimen to degludec in patients with type 1 and type 2 diabetes mellitus. This 24-week open-label intervention study included type 1 (n = 10) and type 2 (n = 20) diabetes mellitus patients, with adequately controlled hemoglobin A1c (HbA1c), who had received insulin glargine or detemir for at least 6 months. The primary outcome was change of QOL from baseline, as assessed by the Diabetes Therapy-Related QOL (DTR-QOL) application, after switching from glargine or detemir to degludec. HbA1c and other parameters were also assessed as secondary outcomes. QOL and HbA1c in patients with type 1 diabetes mellitus were unchanged during this study. In patients with type 2 diabetes mellitus, HbA1c did not change, but total DTR-QOL score was significantly improved from baseline after switching to degludec. The DTR-QOL Factor 2, "Anxiety and dissatisfaction with treatment", was significantly improved in patients with type 2 diabetes mellitus and especially in the subgroup receiving basal supported oral therapy (BOT). Switching of the basal insulin regimen from glargine or detemir to degludec significantly improved the QOL of patients with type 2 diabetes mellitus who were receiving BOT, by reducing mental stress or anxiety about their treatment. | 2015 | Journal of pharmaceutical health care and sciences | No DOI | [
{
"LastName": "Okada",
"FirstName": "Morihiro",
"Affiliation": "Department of Pharmacy, Japan Community Healthcare Organization Kanazawa Hospital, Ha-15 Oki-machi, Kanazawa, 920-8610 Japan ; Department of Medicinal Informatics, Graduate School of Medical Sciences, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-8641 Japan."
},
{
"LastName": "Okada",
"FirstName": "Masae",
"Affiliation": "Department of Pharmacy, Japan Community Healthcare Organization Kanazawa Hospital, Ha-15 Oki-machi, Kanazawa, 920-8610 Japan."
},
{
"LastName": "Nishigami",
"FirstName": "Jun",
"Affiliation": "Department of Pharmacy, Japan Community Healthcare Organization Kanazawa Hospital, Ha-15 Oki-machi, Kanazawa, 920-8610 Japan."
},
{
"LastName": "Yamaaki",
"FirstName": "Naoto",
"Affiliation": "Department of Internal Medicine, Japan Community Healthcare Organization Kanazawa Hospital, Ha-15 Oki-machi, Kanazawa, 920-8610 Japan."
},
{
"LastName": "Furukawa",
"FirstName": "Kenji",
"Affiliation": "Department of Internal Medicine, Japan Community Healthcare Organization Kanazawa Hospital, Ha-15 Oki-machi, Kanazawa, 920-8610 Japan."
},
{
"LastName": "Ohyama",
"FirstName": "Kiminori",
"Affiliation": "Department of Internal Medicine, Japan Community Healthcare Organization Kanazawa Hospital, Ha-15 Oki-machi, Kanazawa, 920-8610 Japan."
},
{
"LastName": "Shimada",
"FirstName": "Tsutomu",
"Affiliation": "Department of Medicinal Informatics, Graduate School of Medical Sciences, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-8641 Japan ; Department of Hospital Pharmacy, University Hospital, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-8641 Japan."
},
{
"LastName": "Sai",
"FirstName": "Yoshimichi",
"Affiliation": "Department of Medicinal Informatics, Graduate School of Medical Sciences, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-8641 Japan ; Department of Hospital Pharmacy, University Hospital, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-8641 Japan."
}
] | No |
37368032 | Isoliquiritigenin attenuates high glucose-induced proliferation, inflammation, and extracellular matrix deposition in glomerular mesangial cells by suppressing JAK2/STAT3 pathway. | To investigate the effect of isoliquiritigenin (ISL) on high glucose (HG)-induced glomerular mesangial cells (GMCs) proliferation, extracellular matrix (ECM) deposition and inflammation, and the underlying mechanisms. Mouse GMCs (SV40-MES-13) were cultured in HG medium, with or without ISL. The proliferation of GMCs was determined by MTT assay. The production of proinflammatory cytokines was detected by qRT-PCR and ELISA. The expression of connective tissue growth factor (CTGF), TGF-β1, collagen IV, and fibronectin was measured by qRT-PCR and western blot. The phosphorylation of JAK2 and STAT3 was examined by western blot. Next, JAK2 inhibitor AG490 was applied to HG-exposed GMCs. The levels of JAK2/STAT3 phosphorylation and pro-fibrotic markers were analyzed by western blot, and the secretion of TNF-α and IL-1β was evaluated by ELISA. GMCs were treated with HG, HG plus ISL or HG plus ISL, and recombinant IL-6 (rIL-6) which is a JAK2 activator. The levels of JAK2/STAT3 activation, ECM formation, and proinflammatory cytokines secretion were determined by western blot and ELISA, respectively. In mouse GMCs, ISL successfully repressed HG-induced hyperproliferation; production of TNF-α and IL-1β; expression of CTGF, TGF-β1, collagen IV, and fibronectin; and activation of JAK2/STAT3. Similar to ISL, AG490 was able to reverse the inflammation and ECM generation caused by HG. Moreover, rIL-6 impeded the amelioration of ISL on HG-induced adverse effects. Our study demonstrated that ISL displayed preventive effects on HG-exposed GMCs through inhibiting JAK2/STAT3 pathway and provided an insight into the application of ISL for diabetic nephropathy (DN) treatment. | 2024 Jan | Naunyn-Schmiedeberg's archives of pharmacology | No DOI | [
{
"LastName": "Zhang",
"FirstName": "Ziyuan",
"Affiliation": "Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang, China."
},
{
"LastName": "Deng",
"FirstName": "Shufen",
"Affiliation": "Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang, China."
},
{
"LastName": "Shi",
"FirstName": "Qiwen",
"Affiliation": "Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang, China. qshi@zjut.edu.cn."
}
] | No |
21753854 | Selective killing of cancer cells by a small molecule targeting the stress response to ROS. | Malignant transformation, driven by gain-of-function mutations in oncogenes and loss-of-function mutations in tumour suppressor genes, results in cell deregulation that is frequently associated with enhanced cellular stress (for example, oxidative, replicative, metabolic and proteotoxic stress, and DNA damage). Adaptation to this stress phenotype is required for cancer cells to survive, and consequently cancer cells may become dependent upon non-oncogenes that do not ordinarily perform such a vital function in normal cells. Thus, targeting these non-oncogene dependencies in the context of a transformed genotype may result in a synthetic lethal interaction and the selective death of cancer cells. Here we used a cell-based small-molecule screening and quantitative proteomics approach that resulted in the unbiased identification of a small molecule that selectively kills cancer cells but not normal cells. Piperlongumine increases the level of reactive oxygen species (ROS) and apoptotic cell death in both cancer cells and normal cells engineered to have a cancer genotype, irrespective of p53 status, but it has little effect on either rapidly or slowly dividing primary normal cells. Significant antitumour effects are observed in piperlongumine-treated mouse xenograft tumour models, with no apparent toxicity in normal mice. Moreover, piperlongumine potently inhibits the growth of spontaneously formed malignant breast tumours and their associated metastases in mice. Our results demonstrate the ability of a small molecule to induce apoptosis selectively in cells that have a cancer genotype, by targeting a non-oncogene co-dependency acquired through the expression of the cancer genotype in response to transformation-induced oxidative stress. | 2011 Jul 13 | Nature | No DOI | [
{
"LastName": "Raj",
"FirstName": "Lakshmi",
"Affiliation": "Cutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, USA."
},
{
"LastName": "Ide",
"FirstName": "Takao",
"Affiliation": ""
},
{
"LastName": "Gurkar",
"FirstName": "Aditi U",
"Affiliation": ""
},
{
"LastName": "Foley",
"FirstName": "Michael",
"Affiliation": ""
},
{
"LastName": "Schenone",
"FirstName": "Monica",
"Affiliation": ""
},
{
"LastName": "Li",
"FirstName": "Xiaoyu",
"Affiliation": ""
},
{
"LastName": "Tolliday",
"FirstName": "Nicola J",
"Affiliation": ""
},
{
"LastName": "Golub",
"FirstName": "Todd R",
"Affiliation": ""
},
{
"LastName": "Carr",
"FirstName": "Steven A",
"Affiliation": ""
},
{
"LastName": "Shamji",
"FirstName": "Alykhan F",
"Affiliation": ""
},
{
"LastName": "Stern",
"FirstName": "Andrew M",
"Affiliation": ""
},
{
"LastName": "Mandinova",
"FirstName": "Anna",
"Affiliation": ""
},
{
"LastName": "Schreiber",
"FirstName": "Stuart L",
"Affiliation": ""
},
{
"LastName": "Lee",
"FirstName": "Sam W",
"Affiliation": ""
}
] | Yes |
9924225 | Prophylactic antiemetic therapy with granisetron in women undergoing thyroidectomy. | We have evaluated the efficacy and safety of granisetron, a selective 5-hydroxytryptamine type-3 receptor antagonist, for the prevention of postoperative nausea and vomiting (PONV) in women undergoing thyroidectomy. In a prospective, randomized, placebo-controlled, double-blind study, 100 ASA I patients, aged 30-57 yr, received placebo or granisetron at three different doses (20, 40 or 100 micrograms kg-1) (n = 25 each), i.v., immediately before induction of anaesthesia. A standard general anaesthetic technique was used. A complete response, defined as no PONV and no need for another rescue antiemetic during the first 3 h after anaesthesia, was seen in 36%, 44%, 92% and 92% of patients who received placebo, granisetron 20 micrograms kg-1, 40 micrograms kg-1 and 100 micrograms kg-1, respectively; corresponding values during the next 21 h after anaesthesia were 40%, 44%, 88%, and 88% (P < 0.05; overall Fisher's exact probability test). There were no clinically important adverse events in any group. We conclude that granisetron 40 micrograms kg-1 was an effective antiemetic for the prevention of PONV after thyroidectomy. Increasing the dose to 100 micrograms kg-1 provided no further benefit. | 1998 Oct | British journal of anaesthesia | No DOI | [
{
"LastName": "Fujii",
"FirstName": "Y",
"Affiliation": "Department of Anaesthesiology, University of Tsukuba Institute of Clinical Medicine, Ibaraki, Japan."
},
{
"LastName": "Saitoh",
"FirstName": "Y",
"Affiliation": ""
},
{
"LastName": "Tanaka",
"FirstName": "H",
"Affiliation": ""
},
{
"LastName": "Toyooka",
"FirstName": "H",
"Affiliation": ""
}
] | Yes |
35155572 | Modeling the MreB-CbtA Interaction to Facilitate the Prediction and Design of Candidate Antibacterial Peptides. | Protein-protein interactions (PPIs) have emerged as promising targets for PPI modulators as alternative drugs because they are essential for most biochemical processes in living organisms. In recent years, a spotlight has been put on the development of peptide-based PPI inhibitors as the next-generation therapeutics to combat antimicrobial resistance taking cognizance of protein-based PPI-modulators that interact with target proteins to inhibit function. Although protein-based PPI inhibitors are not effective therapeutic agents because of their high molecular weights, they could serve as sources for peptide-based pharmaceutics if the target-inhibitor complex is accessible and well characterized. The Escherichia coli (E. coli) toxin protein, CbtA, has been identified as a protein-based PPI modulator that binds to the bacterial actin homolog MreB leading to the perturbation of its polymerization dynamics; and consequently has been suggested to have antibacterial properties. Unfortunately, however, the three-dimensional structures of CbtA and the MreB-CbtA complex are currently not available to facilitate the optimization process of the pharmacological properties of CbtA. In this study, computer modeling strategies were used to predict key MreB-CbtA interactions to facilitate the design of antiMreB peptide candidates. A model of the E. coli CbtA was built using the trRosetta software and its stability was assessed through molecular dynamics (MD) simulations. The modeling and simulations data pointed to a model with reasonable quality and stability. Also, the HADDOCK software was used to predict a possible MreB-CbtA complex, which was characterized through MD simulations and compared with MreB-MreB dimmer. The results suggest that CbtA inhibits MreB through the competitive mechanism whereby CbtA competes with MreB monomers for the interprotofilament interface leading to interference with double protofilament formation. Additionally, by using the antiBP software to predict antibacterial peptides in CbtA, and the MreB-CbtA complex as the reference structure to determine important interactions and contacts, candidate antiMreB peptides were suggested. The peptide sequences could be useful in a rational antimicrobial peptide hybridization strategy to design novel antibiotics. All-inclusive, the data reveal the molecular basis of MreB inhibition by CbtA and can be incorporated in the design/development of the next-generation antibacterial peptides targeting MreB. | 2021 | Frontiers in molecular biosciences | No DOI | [
{
"LastName": "Awuni",
"FirstName": "Elvis",
"Affiliation": "Department of Biochemistry, School of Biological Sciences, CANS, University of Cape Coast, Cape Coast, Ghana."
}
] | No |
30886965 | An evaluation of gout visits in the United States for the years 2007 to 2011. | This study analyzed visits for and factors associated with gout and gout medication treatment trends for the years 2007-2011 in the United States given the introduction of febuxostat, the first new treatment option for gout in over 40 years, which was introduced to the market in 2009. This study was a retrospective, cross-sectional, observational study of patients age 20 and older seen by providers who participated in the National Ambulatory Medical Care Survey (NAMCS), the National Hospital Ambulatory Medical Care Survey Outpatient Department (NHAMCS-OPD) or Emergency Department (NHAMCS-ED) in the United States. The outcome of interest was visits for gout diagnosis and visits where a gout medication was prescribed. Approximately 1.2% of visits had a diagnosis of gout. There was a significant increase in the percentage of visits with a diagnosis of gout in years 2009-2011 compared to 2007-2008, which remained after adjusting for covariates of interest. Groups more likely to have a visit with gout included those ≥65 and 45-64 (both as compared to those 20-44), the African-American and 'Other' race groups (as compared to Caucasians) and those on a diuretic. Groups less likely to have a visit with gout included females, Hispanic/Latinos, those with insurance type of 'Other' and Medicaid (both as compared to private insurance) and visits to a hospital emergency setting (as compared to physician's office visits). Although there was a significant increase in visits where gout is diagnosed across study years, the overall percentage of visits with a gout diagnosis is low in the US population. Treatment trends over the study years has remained consistent, with the introduction of febuxostat appearing to have little impact for the study years through 2011. | 2018 | BMC rheumatology | No DOI | [
{
"LastName": "Castro",
"FirstName": "Kristen E",
"Affiliation": "Campbell University College of Pharmacy & Health Sciences, 180 Main Street PO Box 1090, Buies Creek, NC 27506 USA."
},
{
"LastName": "Corey",
"FirstName": "Kaitlyn D",
"Affiliation": "Campbell University College of Pharmacy & Health Sciences, 180 Main Street PO Box 1090, Buies Creek, NC 27506 USA."
},
{
"LastName": "Raymond",
"FirstName": "Diana L",
"Affiliation": "Campbell University College of Pharmacy & Health Sciences, 180 Main Street PO Box 1090, Buies Creek, NC 27506 USA."
},
{
"LastName": "Jiroutek",
"FirstName": "Michael R",
"Affiliation": "Campbell University College of Pharmacy & Health Sciences, 180 Main Street PO Box 1090, Buies Creek, NC 27506 USA."
},
{
"LastName": "Holland",
"FirstName": "Melissa A",
"Affiliation": "Campbell University College of Pharmacy & Health Sciences, 180 Main Street PO Box 1090, Buies Creek, NC 27506 USA."
}
] | No |
38763432 | 10 weeks low intensity treadmill exercise intervention ameliorates motor deficits and sustains muscle mass via decreasing oxidative damage and increasing mitochondria function in a rat model of Parkinson's disease. | Parkinson's disease (PD) is characterized by loss of dopamine neurons in the brain, which leads to motor dysfunction; excessive inflammation induces neuronal death. This study aimed to determine the most effective exercise modality to improve motor dysfunction in PD by comparing three different exercise regimens (low-intensity treadmill, high-intensity treadmill, and swimming). The rat model for PD was established through stereotaxic surgery, inducing unilateral 6-OHDA (6-hydroxydopamine) lesions. The low-intensity treadmill regimen exerted better protective effects on neurological and motor functions in a rat model of unilateral 6-OHDA-induced PD compared to high-intensity treadmill and swimming. The most suitable exercise regimen and the optimal duration of daily exercise (15 or 30 min) on motor activity and oxidative stress parameters were evaluated. Comparison of 15 and 30 min low-intensity treadmill regimens (10 m/min) revealed 30 min daily exercise was the optimal duration and had more favorable impacts on neurological and motor function. Furthermore, we assessed the neuroprotective effects of exercising for 15 and 30 min per day for either four or ten weeks; 30 min of daily exercise for ten weeks improved mitochondrial function, the antioxidant defense system, neurotrophic factors, and muscle mass, and thereby provided protection against dopaminergic neuron loss, and motor dysfunction in rats with 6-OHDA-induced PD. 30 min of daily low-intensity treadmill exercise over 10 weeks resulted in heightened mitochondrial function in both muscle and brain tissues, therefore, yielded a neuroprotective effect against the loss of dopaminergic neurons and motor dysfunction in PD rats. | 2024 Aug 1 | Life sciences | No DOI | [
{
"LastName": "Tung",
"FirstName": "Yu-Tang",
"Affiliation": "Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan; Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan; Advanced Plant and Food Crop Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan. Electronic address: peggytung@nchu.edu.tw."
},
{
"LastName": "Liao",
"FirstName": "Yi-Chi",
"Affiliation": "Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition, Taipei Medical University, Taipei 110, Taiwan. Electronic address: ma48109004@tmu.edu.tw."
},
{
"LastName": "Yeh",
"FirstName": "Tu-Hsueh",
"Affiliation": "Department of Neurology, Taipei Medical University Hospital, Taipei 110, Taiwan; Department of Neurology, College of Medicine and Taipei Neuroscience Institute, Taipei Medical University, Taipei 110, Taiwan. Electronic address: do2739@tmu.edu.tw."
},
{
"LastName": "Tsao",
"FirstName": "Shu-Ping",
"Affiliation": "Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan. Electronic address: d343105004@tmu.edu.tw."
},
{
"LastName": "Chang",
"FirstName": "Chun-Chao",
"Affiliation": "Division of Gastroenterology and Hepatology, Department of Internal Medicine 110, Taipei Medical University Hospital, Taipei, Taiwan; Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. Electronic address: Chunchao@tmu.edu.tw."
},
{
"LastName": "Shih",
"FirstName": "Wei-Ting",
"Affiliation": "Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition, Taipei Medical University, Taipei 110, Taiwan. Electronic address: ma48110003@tmu.edu.tw."
},
{
"LastName": "Huang",
"FirstName": "Hui-Yu",
"Affiliation": "Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition, Taipei Medical University, Taipei 110, Taiwan; Neuroscience Research Center, Taipei Medical University, Taipei 110, Taiwan; TMU Research Center for Digestive Medicine, Taipei Medical University, Taipei 110, Taiwan. Electronic address: maggieh323@tmu.edu.tw."
}
] | No |
32738155 | Immunohistochemical characterization of immune cell infiltration in paediatric and adult Langerhans cell histiocytosis. | Langerhans cell histiocytosis (LCH) is an inflammatory myeloid neoplasia commonly affecting children with frequent somatic mutations in MAPK pathway genes including BRAF[V600E] and MAP2K1. Some studies suggest that LCH cells can recruit and modulate inflammatory cells, which could provide reciprocal survival signals. To characterize the immune profile of infiltrating inflammatory cells, and to clarify their participation in LCH pathogenesis, a detailed immunohistochemical analysis was performed. Fifteen (10 children, 5 adults) LCH cases were assessed through macrophage (CD68 and CD163), mature dendritic cell (mDC; CD83 and CD208), regulatory T cell (Treg; CD4, CD25 and FOXP3) and cytotoxic lymphocyte (CL; CD56, CD57, perforin and granzyme B) immunomarkers. Moreover, lymphocytic and LCH markers were also analysed. All cases were S100, CD1a, CD207 and CD4-positive. Bcl-2 and cyclin D1 expression was observed in 13 of 15 cases. In the immune microenvironment, M2-polarized macrophages and Tregs were the predominant cell populations, followed by significantly (P < .005) smaller levels of mDCs and CLs. Additionally, the number of CD3 + cells was significantly higher than that of CD20 + cells. In the CD3 + cell population, there were a significantly higher number of CD4 + cells than CD8 + cells. While there were no differences when comparing the paediatric and adult populations, FOXP3 + cells were significantly higher in patients with multisystem involvement and treated with chemotherapy, than single-site cases and those without chemotherapy. Our results suggest that M2-polarized macrophages and Treg infiltration can promote LCH development and survival, probably through pro-tumoral, immunosuppressive and/or cytokine-mediated mechanisms. This work highlights the need for further exploration of immune-targeted therapy for LCH. | 2020 Dec | Scandinavian journal of immunology | No DOI | [
{
"LastName": "Paredes",
"FirstName": "Silvia Elena Yacarini",
"Affiliation": "Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of Sao Paulo (FORP/USP), Ribeirão Preto, Brazil."
},
{
"LastName": "Almeida",
"FirstName": "Luciana Yamamoto",
"Affiliation": "Department of Clinical Medicine, Hematology Division, Ribeirão Preto Medical School (FMRP/USP), University of São Paulo, Ribeirão Preto, Brazil."
},
{
"LastName": "Trevisan",
"FirstName": "Glauce Lunardelli",
"Affiliation": "Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School (FMRP/USP), University of São Paulo, Ribeirão Preto, Brazil."
},
{
"LastName": "Polanco",
"FirstName": "Xiomara Beatriz Jimenez",
"Affiliation": "Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of Sao Paulo (FORP/USP), Ribeirão Preto, Brazil."
},
{
"LastName": "Silveira",
"FirstName": "Heitor Albergoni",
"Affiliation": "Oral Pathology, Department of Stomatology, Public Oral Health and Forensic Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (FORP/USP), Ribeirão Preto, Brazil."
},
{
"LastName": "Vilela Silva",
"FirstName": "Evânio",
"Affiliation": "Oral Pathology, Department of Stomatology, Public Oral Health and Forensic Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (FORP/USP), Ribeirão Preto, Brazil."
},
{
"LastName": "Segato",
"FirstName": "Raquel Assed Bezerra",
"Affiliation": "Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of Sao Paulo (FORP/USP), Ribeirão Preto, Brazil."
},
{
"LastName": "da Silva",
"FirstName": "Léa Assed Bezerra",
"Affiliation": "Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of Sao Paulo (FORP/USP), Ribeirão Preto, Brazil."
},
{
"LastName": "Chahud",
"FirstName": "Fernando",
"Affiliation": "Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School (FMRP/USP), University of São Paulo, Ribeirão Preto, Brazil."
},
{
"LastName": "León",
"FirstName": "Jorge Esquiche",
"Affiliation": "Oral Pathology, Department of Stomatology, Public Oral Health and Forensic Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (FORP/USP), Ribeirão Preto, Brazil."
}
] | No |
30989694 | Vinculin promotes gastric cancer proliferation and migration and predicts poor prognosis in patients with gastric cancer. | Vinculin is a highly conserved protein involved in cell proliferation, migration, and adhesion. However, the effects of vinculin on gastric cancer (GC) remain unclear. Therefore, we aimed to explore the functional role of vinculin in GC, as well as its underlying mechanism. Expression of vinculin in patients with GC was analyzed by real-time polymerase chain reaction, Western blot analysis, and immunohistochemistry. Overall survival was evaluated by the Kaplan-Meier method with the log-rank test. The relationship between vinculin and clinicopathological characteristics of patients with GC was further identified. In addition, we assessed the expression of vinculin in GC cell lines. Besides, vinculin was suppressed or overexpressed by transfection with small interfering (si-vinculin) or pcDNA-vinculin and then cell viability, cell apoptosis, and/or migration was respectively examined by the 3-(4, 5-dimethylthiazole-2-yl)-2, 5-biphenyl tetrazolium bromide assay, flow cytometer, and scratch assay, respectively. Moreover, the cell cycle- and apoptosis-related proteins were detected by Western blot analysis. The expression of vinculin was significantly increased in the GC tissues and cells compared with the nontumor tissues or cells. Vinculin protein positive staining was mainly located in the cell membrane and cytoplasm. Moreover, vinculin was significantly associated with Tumor Node Metastasis (TNM) and poor differentiation. Patients with high vinculin levels had significantly worse overall survival than those with low levels. Suppression of vinculin significantly decreased cell viability and migration and promoted cell apoptosis. However, overexpression of vinculin statistically increased cell viability but had no effects on cell apoptosis. Vinculin promotes GC proliferation and migration and predicts poor prognosis in patients with GC. | 2019 Aug | Journal of cellular biochemistry | No DOI | [
{
"LastName": "Zhang",
"FirstName": "Mingming",
"Affiliation": "Department of Gastroenterology, The Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao, Shandong, China."
},
{
"LastName": "Liu",
"FirstName": "Pei",
"Affiliation": "Department of Infectious Diseases, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China."
},
{
"LastName": "Xu",
"FirstName": "Famei",
"Affiliation": "Department of Pathology, Zibo Central Hospital, Zibo, Shandong, China."
},
{
"LastName": "He",
"FirstName": "Yuanlong",
"Affiliation": "Department of Gastroenterology, The Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao, Shandong, China."
},
{
"LastName": "Xie",
"FirstName": "Xiangjun",
"Affiliation": "Department of Gastroenterology, The Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao, Shandong, China."
},
{
"LastName": "Jiang",
"FirstName": "Xiangjun",
"Affiliation": "Department of Gastroenterology, The Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao, Shandong, China."
}
] | Yes |
28620927 | Bendamustine for relapsed blastic plasmacytoid dendritic cell leukaemia. | Optimal treatment of blastic plasmacytoid dendritic cell neoplasm (BPDCN), a rare entity of dismal prognosis previously described as CD4+/CD56+ hematodermic malignancies, is not defined. We report five cases of relapsed BPDCN treated with bendamustine hydrochloride, a well-tolerated bifunctional drug acting as an alkylating and antimetabolite agent. All patients were above the age of 50 years and in advanced disease (early first relapse in two, subsequent relapse in three; multi-organ involvement in four; previous intensive chemotherapy in five; and stem cell transplantation in four). Four patients were evaluable for response. Two failed therapy, one died from tumor lysis syndrome after rapid blast clearance from blood, and one reached and maintained complete remission for 7 months. Bendamustine should be further evaluated in BPDCN. Copyright © 2015 John Wiley & Sons, Ltd. | 2017 Jun | Hematological oncology | No DOI | [
{
"LastName": "Bétrian",
"FirstName": "Sarah",
"Affiliation": "Hematology Department, Institut Universitaire du Cancer Toulouse-Oncopole, Toulouse, France."
},
{
"LastName": "Guenounou",
"FirstName": "Sarah",
"Affiliation": "Hematology Department, Institut Universitaire du Cancer Toulouse-Oncopole, Toulouse, France."
},
{
"LastName": "Luquet",
"FirstName": "Isabelle",
"Affiliation": "Hematology Laboratory, Institut Universitaire du Cancer Toulouse-Oncopole, Toulouse, France."
},
{
"LastName": "Demur",
"FirstName": "Cécile",
"Affiliation": "Hematology Laboratory, Institut Universitaire du Cancer Toulouse-Oncopole, Toulouse, France."
},
{
"LastName": "Huynh",
"FirstName": "Anne",
"Affiliation": "Hematology Department, Institut Universitaire du Cancer Toulouse-Oncopole, Toulouse, France."
},
{
"LastName": "Ysebaert",
"FirstName": "Loïc",
"Affiliation": "Hematology Department, Institut Universitaire du Cancer Toulouse-Oncopole, Toulouse, France."
},
{
"LastName": "Recher",
"FirstName": "Christian",
"Affiliation": "Hematology Department, Institut Universitaire du Cancer Toulouse-Oncopole, Toulouse, France."
},
{
"LastName": "Huguet",
"FirstName": "Françoise",
"Affiliation": "Hematology Department, Institut Universitaire du Cancer Toulouse-Oncopole, Toulouse, France."
}
] | No |
33890786 | Marine Macroalgal Polygalactan-Built Nanoparticle Construct for Osteogenesis. | Naturally derived polysaccharide biopolymer-based nanoparticles with their size and drug release potentials have appeared as promising biomaterials for osteogenic differentiation. A metallic nanoparticle (GS-AgNP) prepared from a sulfated polygalactan characterized as →3)-2-O-methyl-O-6-sulfonato-β-d-galactopyranosyl-(1 → 4)-2-O-methyl-3,6-anhydro-α-d-galactopyranose-(1→ isolated from the marine macroalga Gracilaria salicornia exhibited a prospective osteogenic effect. Upon treatment with the studied GS-AgNP, alkaline phosphatase activity (88.9 mU/mg) was significantly elevated in human mesenchymal osteoblast stem cells (hMSCs) compared to that in the normal control (33.7 mU/mg). A mineralization study of GS-AgNPs demonstrated an intense mineralized nodule formation on the hMSC surface. A fluorescence-activated cell sorting study of osteocalcin and bone morphogenic protein-2 (BMP-2) expression resulted in an increased population of osteocalcin (78.64%) and BMP-2-positive cells (46.10%) after treatment with GS-AgNPs (250 μg/mL) on M2 macrophages. A time-dependent cell viability study of GS-AgNPs exhibited its non-cytotoxic nature. The studied polygalactan-built nanoparticle could be developed as a promising bioactive pharmacophore against metabolic bone disorder and the treatment for osteogenesis therapy. | 2021 May 10 | Biomacromolecules | No DOI | [
{
"LastName": "Chakraborty",
"FirstName": "Kajal",
"Affiliation": "Marine Bioprospecting Section of Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North P.O., P.B. No. 1603, Cochin 682018, Kerala State, India."
},
{
"LastName": "Antony",
"FirstName": "Tima",
"Affiliation": "Marine Bioprospecting Section of Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North P.O., P.B. No. 1603, Cochin 682018, Kerala State, India."
},
{
"LastName": "Dhara",
"FirstName": "Shubhajit",
"Affiliation": "Marine Bioprospecting Section of Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North P.O., P.B. No. 1603, Cochin 682018, Kerala State, India."
}
] | Yes |
27420918 | Long-Term Outcomes of Hepatectomy for Bilateral Hepatolithiasis with Three-Dimensional Reconstruction: A Propensity Score Matching Analysis. | Bilateral hepatolithiasis tends to show recurrent attacks, which may lead to liver damage and bile duct cancer. This study aimed to assess long-term outcome of hepatectomy for bilateral hepatolithiasis based on three-dimensional reconstruction. A total of 258 bilateral hepatolithiasis patients were enrolled in this retrospective study from January 2008 to October 2014. There are 74 individuals in Three-Divisional Visualization System (MI-3DVS) group (group A) and 74 individuals in non-MI-3DVS group (group B) after propensity score matching (PSM), respectively. Then, complication incidence, stone clearance, recurrence, and recurrence-free survival rates were compared between the two groups. Multivariate Cox analysis was used to test impact of MI-3DVS on recurrence-free survival (RFS). The Median (Range) volumes of intraoperative blood loss were 300 (30-1050) and 400 (50-1800) mL in group A and B, respectively (P = .037). Meanwhile, immediate clearance (77% versus 53%, P = .002) and final clearance (95% versus 80%, P = .007) rates were higher in group A compared with values obtained for group B. Multivariate Cox analysis showed non-MI-3DVS (HR = 2.00, 95% confidence interval [CI] = 1.02-3.95, P = .045) and patients with bilateral cirrhosis (Hazard Ratio [HR] = 10.60, 95% CI = 4.45-25.25, P < .001) were independent predictors of RFS. Compared with conventional radiological technology, MI-3DVS-based hepatectomy resulted in higher stone clearance rate of bilateral hepatolithiasis patients. Non-MI-3DVS and patients with bilateral cirrhosis were independent predictors for recurrence-free survival. | 2016 Sep | Journal of laparoendoscopic & advanced surgical techniques. Part A | No DOI | [
{
"LastName": "Guan",
"FirstName": "Tianpei",
"Affiliation": "Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University , Guangzhou, China ."
},
{
"LastName": "Fang",
"FirstName": "Chihua",
"Affiliation": "Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University , Guangzhou, China ."
},
{
"LastName": "Mo",
"FirstName": "Zhikang",
"Affiliation": "Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University , Guangzhou, China ."
},
{
"LastName": "Xiang",
"FirstName": "Nan",
"Affiliation": "Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University , Guangzhou, China ."
},
{
"LastName": "Yang",
"FirstName": "Jian",
"Affiliation": "Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University , Guangzhou, China ."
},
{
"LastName": "Zeng",
"FirstName": "Ning",
"Affiliation": "Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University , Guangzhou, China ."
}
] | Yes |
19964763 | Pulse-clamp technique for single neuron stimulation electrode characterization. | Miniaturized electrodes, structures and devices are necessary to achieve high target selectivity during stimulation in single neuron networks, while significant charge transfer is still demanded. A reliable test method is required to evaluate charge injection capability for high resolution neural stimulation applications that demand both a large amount of charge injection and a small electrode size. A circuit designed for the pulse-clamp technique was employed to characterize the electrode charge-storage capability of microelectrodes of sizes smaller than 300 microm in diameter. The circuit allows different electrodes and surface modifications to be quickly and accurately compared. Pulse-clamp measurements are performed on planar microelectrodes in 154 mM phosphate buffered saline (PBS) solution with 400 micros long pulses at charges up to 40 nC. The pulse-clamp and cyclic voltammetry results of sputtered iridium oxide film (SIROF) electrodes of different sizes show charge losses of less than 3% and a superior reversible charge injection capability compared to platinum microelectrodes of the same size, even at higher charge density levels. | 2009 | Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference | No DOI | [
{
"LastName": "van Ooyen",
"FirstName": "Andre",
"Affiliation": "Institute of Materials in Electrical Engineering I, RWTH Aachen University, 52074 Aachen, Germany. vanooyen@iwe1.rwth-aachen.de"
},
{
"LastName": "Zagolla",
"FirstName": "Volker G",
"Affiliation": ""
},
{
"LastName": "Ulrich",
"FirstName": "Christian",
"Affiliation": ""
},
{
"LastName": "Schnakenberg",
"FirstName": "Uwe",
"Affiliation": ""
}
] | No |
19031646 | In vitro comparative study of manual and mechanical rotary instrumentation of root canals using computed tomography. | This in vitro study compared, using computed tomography (CT), the amount of dentin removed from root canal walls by manual and mechanical rotary instrumentation techniques. Forty mandibular incisors with dental crown and a single canal were selected. The teeth were randomly assigned to two groups, according to the technique used for root canal preparation: Group I - manual instrumentation with stainless steel files; Group II - mechanical instrumentation with RaCe rotary nickel-titanium instruments. In each tooth, root dentin thickness of the buccal, lingual, mesial and distal surfaces in the apical, middle and cervical thirds of the canal was measured (in mm) using a multislice CT scanner (Siemens Emotion, Duo). Data were stored in the SPSS v. 11.5 and SigmaPlot 2001 v. 7.101 softwares. After crown opening, working length was determined, root canals were instrumented and new CT scans were taken for assessment of root dentin thickness. Pre- and post-instrumentation data were compared and analyzed statistically by ANOVA and Tukey's post-hoc test for significant differences (p=0.05). Based on the findings of this study, it may be concluded that regarding dentin removal from root canal walls during instrumentation, neither of the techniques can be considered more effective than the other. | 2007 | Brazilian dental journal | No DOI | [
{
"LastName": "Limongi",
"FirstName": "Orlando",
"Affiliation": "School of Dentistry, Lutheran University of Brazil, Canoas, RS, Brazil."
},
{
"LastName": "de Albuquerque",
"FirstName": "Diana Santana",
"Affiliation": ""
},
{
"LastName": "Baratto Filho",
"FirstName": "Flares",
"Affiliation": ""
},
{
"LastName": "Vanni",
"FirstName": "José Roberto",
"Affiliation": ""
},
{
"LastName": "de Oliveira",
"FirstName": "Elias P Motcy",
"Affiliation": ""
},
{
"LastName": "Barletta",
"FirstName": "Fernando Branco",
"Affiliation": ""
}
] | No |
17365020 | Anti-inflammatory activity of 4-arylcoumarins from endophytic Streptomyces aureofaciens CMUAc130 in murine macrophage RAW 264.7 cells. | This research was undertaken to test the in vitro anti-inflammatory action of 5,7,4'-trimethoxy-4-phenylcoumarin and 5,7-dimethoxy-4-phenylcoumarin produced by Streptomyces aureofaciens CMUAc130. The effects of the two coumarins were investigated on the formation of NO, PGE2, and TNF-alpha and also on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells. The data obtained were consistent with the modulation of iNOS enzyme expression. A similar effect was also observed when LPS-induced PGE2 release and COX-2 expression were tested. The inhibitory effects were shown in concentration-dependent manners. The 5,7,4'-Trimethoxy-4-phenylcoumarin and 5,7-dimethoxy-4-phenylcoumarin also mildly but significantly reduced the formation of TNF-alpha. | 2007 | Immunological investigations | No DOI | [
{
"LastName": "Taechowisan",
"FirstName": "Thongchai",
"Affiliation": "Department of Microbiology, Faculty of Science, Silpakorn University, Nakorn, Pathom, Thailand. tthongch@su.ac.th"
},
{
"LastName": "Tuntiwachwuttikul",
"FirstName": "Pittaya",
"Affiliation": ""
},
{
"LastName": "Lu",
"FirstName": "Chunhua",
"Affiliation": ""
},
{
"LastName": "Shen",
"FirstName": "Yuemao",
"Affiliation": ""
},
{
"LastName": "Lumyong",
"FirstName": "Saisamorn",
"Affiliation": ""
},
{
"LastName": "Taylor",
"FirstName": "Walter C",
"Affiliation": ""
}
] | Yes |
35478990 | Long Noncoding RNA TFAP2A-AS1 Suppressed Hepatitis B Virus Replication by Modulating miR-933/HDAC11. | Studies have shown that long noncoding RNAs (lncRNAs) play crucial roles in multiple tumor types and regulate various biological processes. The present study tried to study lncRNA TFAP2A-AS1 in HBV infection hepatocellular carcinoma. The level of TFAP2A-AS1 and miR-933 in HCC cell and samples were detected by qRT-PCR assay. Luciferase reporter gene assay was carried out to study the mechanism of TFAP2A-AS1 and miR-933. Cell proliferation was measured by CCK-8 assay. HBV DNA replication was detected by RT-qPCR. We firstly demonstrated that TFAP2A-AS1 was downregulated in HCC cell lines and HBV-infected HCC samples compared with nontumor tissues. However, miR-933 was upregulated in HCC cell lines and HBV-infected HCC samples compared with nontumor tissues, and miR-933 was negatively associated with the expression of TFAP2A-AS1 in HBV-correlated HCC samples. TFAP2A-AS1 and HDAC11 expression was decreased and miR-933 was upregulated in the HBV-infected cell HepG2.2.15. TFAP2A-AS1 acted as a sponge for miR-933 and HDAC11 was one direct target gene for miR-933. Overexpression of TFAP2A-AS1 suppressed cell growth, HBV DNA replication, HbeAg, and HbsAg expression, while knockdown of TFAP2A-AS1 enhanced cell proliferation, HBV DNA replication, HbeAg, and HbsAg expression in HepG2.2.15 cell. In addition, ectopic expression of miR-933 promoted cell growth, HBV DNA replication, HbeAg, and HbsAg expression in HepG2.2.15 cell. TFAP2A-AS1 suppressed HBV replication and infection through regulating HDAC11. These data demonstrated that TFAP2A-AS1 acted crucial roles in the modulation of HbeAg and HbsAg expression and HBV replication and may be one potential target for HBV infection treatment. | 2022 | Disease markers | No DOI | [
{
"LastName": "Cheng",
"FirstName": "Yu",
"Affiliation": "Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin, 150001 Heilongjiang, China."
},
{
"LastName": "Shi",
"FirstName": "Weiwu",
"Affiliation": "Department of integrated internal medicine, Shanghai Donghai Senior Nursing Hospital, Shanghai, China."
},
{
"LastName": "Cui",
"FirstName": "Xudong",
"Affiliation": "Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin, 150001 Heilongjiang, China."
},
{
"LastName": "Sun",
"FirstName": "Lei",
"Affiliation": "Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin, 150001 Heilongjiang, China."
},
{
"LastName": "Nan",
"FirstName": "Yi",
"Affiliation": "Traditional Chinese Medicine College of Ningxia Medical University, Yinchuan 750004, China."
},
{
"LastName": "Yao",
"FirstName": "Hong",
"Affiliation": "Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin, 150001 Heilongjiang, China."
},
{
"LastName": "Fan",
"FirstName": "Jian",
"Affiliation": "Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin, 150001 Heilongjiang, China."
},
{
"LastName": "Zhu",
"FirstName": "LiYing",
"Affiliation": "Department of Infectious Disease, The Second Hospital of Harbin Medical University, Harbin, 150000 Heilongjiang, China."
},
{
"LastName": "Yu",
"FirstName": "Lei",
"Affiliation": "Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin, 150001 Heilongjiang, China."
}
] | Yes |
30049709 | Novel Campylobacter concisus lipooligosaccharide is a determinant of inflammatory potential and virulence. | The pathogenicity of Campylobacter concisus, increasingly found in the human gastrointestinal (GI) tract, is unclear. Some studies indicate that its role in GI conditions has been underestimated, whereas others suggest that the organism has a commensal-like phenotype. For the enteropathogen C. jejuni, the lipooligosaccharide (LOS) is a main driver of virulence. We investigated the LOS structure of four C. concisus clinical isolates and correlated the inflammatory potential of each isolate with bacterial virulence. Mass spectrometric analyses of lipid A revealed a novel hexa-acylated diglucosamine moiety with two or three phosphoryl substituents. Molecular and fragment ion analysis indicated that the oligosaccharide portion of the LOS had only a single phosphate and lacked phosphoethanolamine and sialic acid substitution, which are hallmarks of the C. jejuni LOS. Consistent with our structural findings, C. concisus LOS and live bacteria induced less TNF-α secretion in human monocytes than did C. jejuni Furthermore, the C. concisus bacteria were less virulent than C. jejuni in a Galleria mellonella infection model. The correlation of the novel lipid A structure, decreased phosphorylation, and lack of sialylation along with reduced inflammatory potential and virulence support the significance of the LOS as a determinant in the relative pathogenicity of C. concisus. | 2018 Oct | Journal of lipid research | No DOI | [
{
"LastName": "Brunner",
"FirstName": "Katja",
"Affiliation": "Infection, Immunity and Inflammation Programme, University College London Great Ormond Street Institute of Child Health, London, United Kingdom."
},
{
"LastName": "John",
"FirstName": "Constance M",
"Affiliation": "Center for Immunochemistry, Veterans Affairs Medical Center, San Francisco, CA."
},
{
"LastName": "Phillips",
"FirstName": "Nancy J",
"Affiliation": "Department of Pharmaceutical Chemistry, University of California, San Francisco, CA."
},
{
"LastName": "Alber",
"FirstName": "Dagmar G",
"Affiliation": "Infection, Immunity and Inflammation Programme, University College London Great Ormond Street Institute of Child Health, London, United Kingdom."
},
{
"LastName": "Gemmell",
"FirstName": "Matthew R",
"Affiliation": "Center for Genome-Enabled Biology and Medicine, School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom."
},
{
"LastName": "Hansen",
"FirstName": "Richard",
"Affiliation": "Department of Paediatric Gastroenterology, Royal Hospital for Children, Glasgow, United Kingdom."
},
{
"LastName": "Nielsen",
"FirstName": "Hans L",
"Affiliation": "Department of Infectious Diseases Aalborg University Hospital, Aalborg, Denmark."
},
{
"LastName": "Hold",
"FirstName": "Georgina L",
"Affiliation": "St George and Sutherland Clinical School, University of New South Wales, Sydney, Australia."
},
{
"LastName": "Bajaj-Elliott",
"FirstName": "Mona",
"Affiliation": "Infection, Immunity and Inflammation Programme, University College London Great Ormond Street Institute of Child Health, London, United Kingdom."
},
{
"LastName": "Jarvis",
"FirstName": "Gary A",
"Affiliation": "Center for Immunochemistry, Veterans Affairs Medical Center, San Francisco, CA gary.jarvis@ucsf.edu."
}
] | No |
35819585 | Audio-Visual Automatic Speech Recognition Towards Education for Disabilities. | Education is a fundamental right that enriches everyone's life. However, physically challenged people often debar from the general and advanced education system. Audio-Visual Automatic Speech Recognition (AV-ASR) based system is useful to improve the education of physically challenged people by providing hands-free computing. They can communicate to the learning system through AV-ASR. However, it is challenging to trace the lip correctly for visual modality. Thus, this paper addresses the appearance-based visual feature along with the co-occurrence statistical measure for visual speech recognition. Local Binary Pattern-Three Orthogonal Planes (LBP-TOP) and Grey-Level Co-occurrence Matrix (GLCM) is proposed for visual speech information. The experimental results show that the proposed system achieves 76.60 % accuracy for visual speech and 96.00 % accuracy for audio speech recognition. | 2023 Sep | Journal of autism and developmental disorders | No DOI | [
{
"LastName": "Debnath",
"FirstName": "Saswati",
"Affiliation": "Department of Computer Science and Engineering, Alliance University, Bangalore, Karnataka, India."
},
{
"LastName": "Roy",
"FirstName": "Pinki",
"Affiliation": "Department of Computer Science and Engineering, National Institute of Technology, Silchar, Assam, India."
},
{
"LastName": "Namasudra",
"FirstName": "Suyel",
"Affiliation": "Department of Computer Science and Engineering, National Institute of Technology Patna, Patna, Bihar, India. suyelnamasudra@gmail.com."
},
{
"LastName": "Crespo",
"FirstName": "Ruben Gonzalez",
"Affiliation": "Universidad Internacional de La Rioja, Logroño, Spain."
}
] | Yes |
Subsets and Splits