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33615468 | Misoprostol versus expectant management in women with incomplete first-trimester miscarriage after failed primary misoprostol treatment: A randomized clinical trial. | To compare the effectiveness and safety of repeat misoprostol versus expectant management in women with first-trimester incomplete miscarriage who have been initially treated with misoprostol. The study was an open-labeled randomized controlled trial including women with an incomplete first-trimester miscarriage after administration of misoprostol. The participants were randomly assigned to vaginal misoprostol or expectant management using a computer-generated table of random numbers. The primary outcome was the number of women with a complete miscarriage at 1 week. Eighty-eight women (44 women in each group) were analyzed. The rate of complete miscarriage at 1 week was significantly higher in the misoprostol group than the expectant management group-29 (69.0%) versus 7 (16.7%) (P < 0.001), respectively. Women in the misoprostol group were more satisfied (7.00 ± 0.77 vs 4.57 ± 1.61, P < 0.001) but reported more pain (7.95 ± 1.85 vs 5.26 ± 1.08, P < 0.001) than women in the expectant group. The misoprostol group reported more adverse effects than the expectant management group (P < 0.001). In women with an incomplete first-trimester miscarriage who were initially treated with misoprostol, repeat administration of misoprostol was more effective than expectant management for achieving complete miscarriage at 1 week. However, misoprostol was associated with more adverse effects. Clinicaltrials.gov: NCT03148561. | 2021 Sep | International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics | No DOI | [
{
"LastName": "Ali",
"FirstName": "Mohammed K",
"Affiliation": "Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Egypt."
},
{
"LastName": "Emam",
"FirstName": "Samar M",
"Affiliation": "Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Egypt."
},
{
"LastName": "Abdel-Aleem",
"FirstName": "Mahmoud A",
"Affiliation": "Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Egypt."
},
{
"LastName": "Sobh",
"FirstName": "Ahmed M A",
"Affiliation": "Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Egypt."
}
] | Yes |
38412071 | The identification of metabolites from gut microbiota in coronary heart disease via network pharmacology. | Although the gut microbial metabolites exhibit potential effects on coronary heart disease (CHD), the underlying mechanism remains unclear. In this study, the active gut microbial metabolites acting on CHD and their potential mechanisms of action were explored through a network pharmacological approach. We collected a total of 208 metabolites from the gutMgene database and 726 overlapping targets from the similarity ensemble approach (SEA) and SwissTargetPrediction (STP) database, and ultimately identified 610 targets relevant to CHD. In conjunction with the gutMGene database, we identified 12 key targets. The targets of exogenous substances were removed, and 10 core targets involved in CHD were eventually retained. The microbiota-metabolites-targets-signalling pathways network analysis revealed that C-type lectin receptor signalling pathway, Lachnospiraceae, Escherichia, mitogen-activated protein kinase 1, prostaglandin-endoperoxidase synthase 2, phenylacetylglutamine and alcoholic acid are notable components of CHD and play important roles in the development of CHD. The results of molecular docking experiments demonstrated that AKT1-glycocholic acid and PTGS2-phenylacetylglutamine complexes may act on C-type lectin receptor signalling pathways. In this study, the key substances and potential mechanisms of gut microbial metabolites were analysed via network pharmacological methods, and a scientific basis and comprehensive idea were provided for the effects of gut microbial metabolites on CHD. | 2024 Dec | Artificial cells, nanomedicine, and biotechnology | No DOI | [
{
"LastName": "Zhou",
"FirstName": "Hao-Ming",
"Affiliation": "Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xi'an, China."
},
{
"LastName": "Yang",
"FirstName": "Xin-Yu",
"Affiliation": "Department of Pharmacy, Beijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use, Beijing Shijitan Hospital, Capital Medical University, Beijing, China."
},
{
"LastName": "Yue",
"FirstName": "Shi-Jun",
"Affiliation": "Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xi'an, China."
},
{
"LastName": "Wang",
"FirstName": "Wen-Xiao",
"Affiliation": "International Joint Research Center on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province, Hebei University of Chinese Medicine, Shijiazhuang, China."
},
{
"LastName": "Zhang",
"FirstName": "Qiao",
"Affiliation": "Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xi'an, China."
},
{
"LastName": "Xu",
"FirstName": "Ding-Qiao",
"Affiliation": "Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xi'an, China."
},
{
"LastName": "Li",
"FirstName": "Jia-Jia",
"Affiliation": "Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xi'an, China."
},
{
"LastName": "Tang",
"FirstName": "Yu-Ping",
"Affiliation": "Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xi'an, China."
}
] | No |
29390191 | Inducible Knockdown of Endothelial Protein Tyrosine Phosphatase-1B Promotes Neointima Formation in Obese Mice by Enhancing Endothelial Senescence. | Protein tyrosine phosphatase-1B (PTP1B) is a negative regulator of receptor tyrosine kinase signaling. In this study, we determined the importance of PTP1B expressed in endothelial cells for the vascular response to arterial injury in obesity. Morphometric analysis of vascular lesions generated by 10% ferric chloride (FeCl3) revealed that tamoxifen-inducible endothelial PTP1B deletion (Tie2.ER[T2]-Cre × PTP1B[fl/fl]; End.PTP1B knockout, KO) significantly increased neointima formation, and reduced numbers of (endothelial lectin-positive) luminal cells in End.PTP1B-KO mice suggested impaired lesion re-endothelialization. Significantly higher numbers of proliferating cell nuclear antigen (PCNA)-positive proliferating cells as well as smooth muscle actin (SMA)-positive or vascular cell adhesion molecule-1 (VCAM1)-positive activated smooth muscle cells or vimentin-positive myofibroblasts were detected in neointimal lesions of End.PTP1B-KO mice, whereas F4/80-positive macrophage numbers did not differ. Activated receptor tyrosine kinase and transforming growth factor-beta (TGFβ) signaling and oxidative stress markers were also significantly more abundant in End.PTP1B-KO mouse lesions. Genetic knockdown or pharmacological inhibition of PTP1B in endothelial cells resulted in increased expression of caveolin-1 and oxidative stress, and distinct morphological changes, elevated numbers of senescence-associated β-galactosidase-positive cells, and increased expression of tumor suppressor protein 53 (p53) or the cell cycle inhibitor cyclin-dependent kinase inhibitor-2A (p16INK4A) suggested senescence, all of which could be attenuated by small interfering RNA (siRNA)-mediated downregulation of caveolin-1. In vitro, senescence could be prevented and impaired re-endothelialization restored by preincubation with the antioxidant Trolox. Our results reveal a previously unknown role of PTP1B in endothelial cells and provide mechanistic insights how PTP1B deletion or inhibition may promote endothelial senescence. Absence of PTP1B in endothelial cells impairs re-endothelialization, and the failure to induce smooth muscle cell quiescence or to protect from circulating growth factors may result in neointimal hyperplasia. | 2019 Mar 1 | Antioxidants & redox signaling | No DOI | [
{
"LastName": "Jäger",
"FirstName": "Marianne",
"Affiliation": "1 Center for Cardiology, Cardiology I, University Medical Center Mainz, Mainz, Germany."
},
{
"LastName": "Hubert",
"FirstName": "Astrid",
"Affiliation": "1 Center for Cardiology, Cardiology I, University Medical Center Mainz, Mainz, Germany."
},
{
"LastName": "Gogiraju",
"FirstName": "Rajinikanth",
"Affiliation": "1 Center for Cardiology, Cardiology I, University Medical Center Mainz, Mainz, Germany."
},
{
"LastName": "Bochenek",
"FirstName": "Magdalena L",
"Affiliation": "1 Center for Cardiology, Cardiology I, University Medical Center Mainz, Mainz, Germany."
},
{
"LastName": "Münzel",
"FirstName": "Thomas",
"Affiliation": "1 Center for Cardiology, Cardiology I, University Medical Center Mainz, Mainz, Germany."
},
{
"LastName": "Schäfer",
"FirstName": "Katrin",
"Affiliation": "1 Center for Cardiology, Cardiology I, University Medical Center Mainz, Mainz, Germany."
}
] | No |
35014678 | A pyridinium‑type fullerene derivative suppresses primary effusion lymphoma cell viability via the downregulation of the Wnt signaling pathway through the destabilization of β‑catenin. | Primary effusion lymphoma (PEL) is defined as a rare subtype of non‑Hodgkin's B cell lymphoma, which is caused by Kaposi's sarcoma‑associated herpesvirus (KSHV) in immunosuppressed patients. PEL is an aggressive type of lymphoma and is frequently resistant to conventional chemotherapeutics. Therefore, the discovery of novel drug candidates for the treatment of PEL is of utmost importance. In order to discover potential novel anti‑tumor compounds against PEL, the authors previously developed a pyrrolidinium‑type fullerene derivative, 1,1,1',1'‑tetramethyl [60]fullerenodipyrrolidinium diiodide (derivative #1), which induced the apoptosis of PEL cells via caspase‑9 activation. In the present study, the growth inhibitory effects of pyrrolidinium‑type (derivatives #1 and #2), pyridinium‑type (derivatives #3 and #5 to #9) and anilinium‑type fullerene derivatives (derivative #4) against PEL cells were evaluated. This analysis revealed a pyridinium‑type derivative (derivative #5; 3‑ 5'‑(etho xycarbonyl)‑1',5'‑dihydro‑2'H‑[5,6]fullereno‑C60‑Ih‑[1,9‑c]pyrrol‑2'‑yl]‑1‑methylpyridinium iodide), which exhibited antitumor activity against PEL cells via the downregulation of Wnt/β‑catenin signaling. Derivative #5 suppressed the viability of KSHV‑infected PEL cells compared with KSHV‑uninfected B‑lymphoma cells. Furthermore, derivative #5 induced the destabilization of β‑catenin and suppressed β‑catenin‑TCF4 transcriptional activity in PEL cells. It is known that the constitutive activation of Wnt/β‑catenin signaling is essential for the growth of KSHV‑infected cells. The Wnt/β‑catenin activation in KSHV‑infected cells is mediated by KSHV latency‑associated nuclear antigen (LANA). The data demonstrated that derivative #5 increased β‑catenin phosphorylation, which resulted in β‑catenin polyubiquitination and subsequent degradation. Thus, derivative #5 overcame LANA‑mediated β‑catenin stabilization. Furthermore, the administration of derivative #5 suppressed the development of PEL cells in the ascites of SCID mice with tumor xenografts derived from PEL cells. On the whole, these findings provide evidence that the pyridinium‑type fullerene derivative #5 exhibits antitumor activity against PEL cells in vitro and in vivo. Thus, derivative #5 may be utilized as a novel therapeutic agent for the treatment of PEL. | 2022 Mar | Oncology reports | No DOI | [
{
"LastName": "Kadota",
"FirstName": "Ayano",
"Affiliation": "Department of Cell Biology, Kyoto Pharmaceutical University, Yamashinaku, Kyoto 607‑8412, Japan."
},
{
"LastName": "Moriguchi",
"FirstName": "Misato",
"Affiliation": "Department of Cell Biology, Kyoto Pharmaceutical University, Yamashinaku, Kyoto 607‑8412, Japan."
},
{
"LastName": "Watanabe",
"FirstName": "Tadashi",
"Affiliation": "Department of Cell Biology, Kyoto Pharmaceutical University, Yamashinaku, Kyoto 607‑8412, Japan."
},
{
"LastName": "Sekine",
"FirstName": "Yuichi",
"Affiliation": "Department of Cell Biology, Kyoto Pharmaceutical University, Yamashinaku, Kyoto 607‑8412, Japan."
},
{
"LastName": "Nakamura",
"FirstName": "Shigeo",
"Affiliation": "Department of Chemistry, Nippon Medical School, Musashino, Tokyo 180‑0023, Japan."
},
{
"LastName": "Yasuno",
"FirstName": "Takumi",
"Affiliation": "Department of Pharmaceutical Sciences, Faculty of Pharmacy, Keio University, Tokyo 105‑8512, Japan."
},
{
"LastName": "Ohe",
"FirstName": "Tomoyuki",
"Affiliation": "Department of Pharmaceutical Sciences, Faculty of Pharmacy, Keio University, Tokyo 105‑8512, Japan."
},
{
"LastName": "Mashino",
"FirstName": "Tadahiko",
"Affiliation": "Department of Pharmaceutical Sciences, Faculty of Pharmacy, Keio University, Tokyo 105‑8512, Japan."
},
{
"LastName": "Fujimuro",
"FirstName": "Masahiro",
"Affiliation": "Department of Cell Biology, Kyoto Pharmaceutical University, Yamashinaku, Kyoto 607‑8412, Japan."
}
] | No |
36275902 | Glutathione Peroxidase 4 Is a Predictor of Diabetic Kidney Disease Progression in Type 2 Diabetes Mellitus. | Diabetic kidney disease (DKD) represents a heavy burden in type 2 diabetes mellitus (T2DM). Ferroptosis plays an important role in DKD, and it thus provides new perspectives to pursue more related biomarkers to assess the disease severity and prognosis. Glutathione peroxidase 4 (GPX4) is the mainstay in regulating ferroptosis. The current study investigated the predictive value of kidney GPX4 expression level in DKD progression. We measured GPX4 levels in kidney paraffin sections of 85 biopsy-proven DKD patients by immunohistochemistry staining. The associations between the GPX4 level and clinicopathological parameters as well as renal outcomes were analyzed. GPX4 is mainly expressed in kidney tubulointerstitium, especially in tubular epithelial cells of DKD patients. The GPX4 expression level was significantly lower in DKD patients than healthy controls. Besides, GPX4 level significantly correlated with proteinuria (r = -0.42, p < 0.001), urinary albumin-to-creatinine ratio (uACR) (r = -0.40, p < 0.01), serum creatinine (Scr) (r = -0.59, p < 0.001), estimated glomerular filtration rate (eGFR) (r = 0.66, p < 0.001), and the percentage of sclerosed glomeruli (r = -0.42, p < 0.001) in renal specimens. During follow-up, the GPX4 level positively correlated with eGFR slope (r = 0.48, p < 0.001), and GPX4-low patients showed a significantly higher probability of developing end-stage kidney disease (ESKD) compared with GPX4-high patients (p < 0.01). Moreover, after adjusting for other potential predictors, the GPX4 level was still an independent predictor of developing ESKD (HR 2.15, 95% CI 1.08 to 4.28, p < 0.05). Kidney tubulointerstitial GPX4 expression level was associated with the disease severity and progression of DKD. | 2022 | Oxidative medicine and cellular longevity | No DOI | [
{
"LastName": "Wang",
"FirstName": "Yi-Hui",
"Affiliation": "Renal Division, Department of Medicine, Peking University First Hospital, Institute of Nephrology, Peking University, Key Laboratory of Renal Disease, Ministry of Health of China, Key Laboratory of Chronic Kidney Disease Prevention and Treatment (Peking University), Ministry of Education, Beijing 100034, China."
},
{
"LastName": "Chang",
"FirstName": "Dong-Yuan",
"Affiliation": "Renal Division, Department of Medicine, Peking University First Hospital, Institute of Nephrology, Peking University, Key Laboratory of Renal Disease, Ministry of Health of China, Key Laboratory of Chronic Kidney Disease Prevention and Treatment (Peking University), Ministry of Education, Beijing 100034, China."
},
{
"LastName": "Zhao",
"FirstName": "Ming-Hui",
"Affiliation": "Renal Division, Department of Medicine, Peking University First Hospital, Institute of Nephrology, Peking University, Key Laboratory of Renal Disease, Ministry of Health of China, Key Laboratory of Chronic Kidney Disease Prevention and Treatment (Peking University), Ministry of Education, Beijing 100034, China."
},
{
"LastName": "Chen",
"FirstName": "Min",
"Affiliation": "Renal Division, Department of Medicine, Peking University First Hospital, Institute of Nephrology, Peking University, Key Laboratory of Renal Disease, Ministry of Health of China, Key Laboratory of Chronic Kidney Disease Prevention and Treatment (Peking University), Ministry of Education, Beijing 100034, China."
}
] | No |
36468336 | Laminin matrix adhesion regulates basal mammary epithelial cell identity. | Mammary epithelium is a bilayered ductal network composed of luminal and basal epithelial cells, which together drive the growth and functional differentiation of the gland. Basal mammary epithelial cells (MECs) exhibit remarkable plasticity and progenitor activity that facilitate epithelial expansion. However, their activity must be tightly regulated to restrict excess basal cell activity. Here, we show that adhesion of basal cells to laminin α5-containing basement membrane matrix, which is produced by luminal cells, presents such a control mechanism. Adhesion to laminin α5 directs basal cells towards a luminal cell fate, and thereby results in a marked decrease of basal MEC progenitor activity in vitro and in vivo. Mechanistically, these effects are mediated through β4-integrin and activation of p21 (encoded by CDKN1A). Thus, we demonstrate that laminin matrix adhesion is a key determinant of basal identity and essential to building and maintaining a functional multicellular epithelium. | 2022 Dec 1 | Journal of cell science | No DOI | [
{
"LastName": "Englund",
"FirstName": "Johanna I",
"Affiliation": "Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki FI-00014, Finland."
},
{
"LastName": "Bui",
"FirstName": "Hien",
"Affiliation": "Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki FI-00014, Finland."
},
{
"LastName": "Dinç",
"FirstName": "Defne D",
"Affiliation": "Institute of Biomedicine, Cancer Laboratory FICAN west, University of Turku, Turku FI-20014, Finland."
},
{
"LastName": "Paavolainen",
"FirstName": "Oona",
"Affiliation": "Institute of Biomedicine, Cancer Laboratory FICAN west, University of Turku, Turku FI-20014, Finland."
},
{
"LastName": "McKenna",
"FirstName": "Tomás",
"Affiliation": "Department of Cell and Molecular Biology (CMB), Karolinska Institutet, Stockholm SE-171 77, Sweden."
},
{
"LastName": "Laitinen",
"FirstName": "Suvi",
"Affiliation": "Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki FI-00014, Finland."
},
{
"LastName": "Munne",
"FirstName": "Pauliina",
"Affiliation": "Finnish Cancer Institute, FICAN South Helsinki University Hospital & Translational Cancer Medicine, Medical Faculty, University of Helsinki, Helsinki FI-00014, Finland."
},
{
"LastName": "Klefström",
"FirstName": "Juha",
"Affiliation": "Finnish Cancer Institute, FICAN South Helsinki University Hospital & Translational Cancer Medicine, Medical Faculty, University of Helsinki, Helsinki FI-00014, Finland."
},
{
"LastName": "Peuhu",
"FirstName": "Emilia",
"Affiliation": "Institute of Biomedicine, Cancer Laboratory FICAN west, University of Turku, Turku FI-20014, Finland."
},
{
"LastName": "Katajisto",
"FirstName": "Pekka",
"Affiliation": "Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki FI-00014, Finland."
}
] | No |
26496463 | Carboplatin and Etoposide in Heavily Pretreated Patients with Progressive High-Grade Glioma. | Treatment of recurrent anaplastic glioma and glioblastoma remains a particular challenge in neurooncology. The lack of controlled trials results in poor evidence for all therapeutic options. Upon recurrence, many patients are treated with bevacizumab or one of the frequently used nitrosoureas such as lomustine. However, patients who still present in overall good condition after failure of multiple lines of therapy may ask for additional therapy. Here, we report our experience with the use of carboplatin in combination with etoposide as fourth- or fifth-line therapy in patients with progressive high-grade glioma. The median Karnofsky performance status at the beginning of treatment was 80%. The median progression-free survival (PFS) was 2.5 months. PFS at 6 months was 0%. Administration of carboplatin and etoposide was associated with grade 3 or 4 hematotoxicity in 8 of 12 patients. Carboplatin in combination with etoposide has an unfavorable risk-benefit profile in heavily pretreated glioma patients. | 2014 | Chemotherapy | No DOI | [
{
"LastName": "Tonder",
"FirstName": "Michaela",
"Affiliation": "Department of Neurology, University Hospital Zurich, University of Zurich, Zurich, Switzerland."
},
{
"LastName": "Weller",
"FirstName": "Michael",
"Affiliation": ""
},
{
"LastName": "Eisele",
"FirstName": "Günter",
"Affiliation": ""
},
{
"LastName": "Roth",
"FirstName": "Patrick",
"Affiliation": ""
}
] | No |
30139377 | Endothelial progenitor cell-derived exosomes, loaded with miR-126, promoted deep vein thrombosis resolution and recanalization. | Deep vein thrombosis (DVT) is caused by blood clotting in the deep veins. Thrombosis resolution and recanalization can be accelerated by endothelial progenitor cells. In this report, we investigated the effects of miR-126-loaded EPC-derived exosomes (miR-126-Exo) on EPCs function and venous thrombus resolution. In vitro promotional effect of miR-126-Exo on the migration and tube incorporation ability of EPCs was investigated via transwell assay and tube formation assay. In addition, a mouse venous thrombosis model was constructed and treated with miR-126-Exo to clarify the therapeutic effect of miR-126-Exo by histological analysis. Lastly, this study predicted a target gene of miR-126 using target prediction algorithms and confirmed it by luciferase activity assay, RT-qPCR, and Western blot. Transwell assay and tube formation assay indicated that miR-126-Exo could enhance the migration and tube incorporation ability of EPCs. Moreover, in vivo study manifested enhanced thrombus organization and recanalization after miR-126-Exo treatment. Meanwhile, we identified that Protocadherin 7 as a target gene of miR-126. To sum up, our results demonstrated that EPC-derived exosomes loaded with miR-126 significantly promoted thrombus resolution in an animal model of venous thrombosis, indicating exosomes as a promising potential vehicle carrying therapeutic molecules for DVT therapy. | 2018 Aug 23 | Stem cell research & therapy | No DOI | [
{
"LastName": "Sun",
"FirstName": "Jiacheng",
"Affiliation": "Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Soochow University, Suzhou, 215000, China."
},
{
"LastName": "Zhang",
"FirstName": "Zhiwei",
"Affiliation": "Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China."
},
{
"LastName": "Ma",
"FirstName": "Teng",
"Affiliation": "Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Soochow University, Suzhou, 215000, China."
},
{
"LastName": "Yang",
"FirstName": "Ziying",
"Affiliation": "Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Soochow University, Suzhou, 215000, China."
},
{
"LastName": "Zhang",
"FirstName": "Jinlong",
"Affiliation": "Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Soochow University, Suzhou, 215000, China."
},
{
"LastName": "Liu",
"FirstName": "Xuan",
"Affiliation": "Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Soochow University, Suzhou, 215000, China."
},
{
"LastName": "Lu",
"FirstName": "Da",
"Affiliation": "Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Soochow University, Suzhou, 215000, China."
},
{
"LastName": "Shen",
"FirstName": "Zhenya",
"Affiliation": "Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Soochow University, Suzhou, 215000, China. zhenyashen@sina.cn."
},
{
"LastName": "Yang",
"FirstName": "Junjie",
"Affiliation": "Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, 35294, Alabama, USA. junjieyang2009@gmail.com."
},
{
"LastName": "Meng",
"FirstName": "Qingyou",
"Affiliation": "Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215000, China. mengqy@163.com."
}
] | Yes |
36111165 | Irisin Ameliorates Oxidative Stress-Induced Injury in Pancreatic Beta-Cells by Inhibiting Txnip and Inducing Stat3-Trx2 Pathway Activation. | Lipotoxicity can lead to beta-cell dysfunction and apoptosis because it induces oxidative stress. Recent studies have found that Irisin prevents pancreatic beta-cell dysfunction induced by palmitic acid (PA). However, an association between the protection against oxidative stress conferred by Irisin and beta-cell dysfunction has not been fully elucidated. In this study, we observed that Irisin treatment prevented INS-1 cell apoptosis induced by PA treatment and preserved the insulin-secreting function of INS-1 cells in vitro. These effects probably resulted from the Irisin-induced decrease in intracellular ROS levels triggered by PA treatment. In addition, PA treatment induced oxidative stress partially by inhibiting the activation of thioredoxin 2 (Trx2) through its increase of thioredoxin-interacting protein (Txnip) expression. However, Irisin administration blocked the increase in Txnip expression, which reversed the PA-induced inactivation of Trx2. Irisin also increased the nuclear translocation of Stat3, and the inhibition of Stat3 by siRNAs blocked Irisin-induced Trx2 expression, indicating that both Txnip and Stat3 are involved in Irisin-induced activation of Trx2. Furthermore, blockade of Stat3 by siRNAs led to the decreased gene expression of MafA and Ins and to cessation of glucose-induced insulin secretion that had been enhanced by Irisin. In vivo, HFD treatment led to reduced glucose tolerance and an increase in the level of the oxidative marker malondialdehyde (MDA) compared to that in the control group. However, these effects were ameliorated by Irisin injection due to the inhibition of beta-cell apoptosis and the activation of Trx2, probably through Txnip inhibition and Stat3 activation. In conclusion, our results reveal a possible mechanism for Irisin-induced beta-cell protection, which is mediated through Txnip inhibition and activation of the Stat3-Trx2 pathway. | 2022 | Oxidative medicine and cellular longevity | No DOI | [
{
"LastName": "Liu",
"FirstName": "Chongxiao",
"Affiliation": "Department of Endocrinology, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China."
},
{
"LastName": "Zhou",
"FirstName": "Jianhua",
"Affiliation": "Department of Endocrinology, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China."
},
{
"LastName": "Xu",
"FirstName": "Yanhong",
"Affiliation": "Department of Endocrinology, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China."
},
{
"LastName": "Gong",
"FirstName": "Sa",
"Affiliation": "Department of Endocrinology, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China."
},
{
"LastName": "Zhu",
"FirstName": "Yi",
"Affiliation": "Department of Endocrinology, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China."
},
{
"LastName": "Zhang",
"FirstName": "Hongli",
"Affiliation": "Department of Endocrinology, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China."
},
{
"LastName": "Dong",
"FirstName": "Yan",
"Affiliation": "Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China."
},
{
"LastName": "Zhao",
"FirstName": "Bingxia",
"Affiliation": "Center of Minimally Invasive Treatment for Tumor, Department of Medical Ultrasound, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, China."
},
{
"LastName": "Li",
"FirstName": "Xiaohua",
"Affiliation": "Department of Endocrinology, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China."
}
] | No |
18333205 | The role of liver transplantation in the treatment of hilar cholangiocarcinoma. | Surgical resection or liver transplantation (LTx) are the only available treatments that offer a potential for long-term survival or cure in cases of hilar cholangiocarcinoma. Hilar resection in combination with partial hepatectomy and caudate lobectomy is regarded as the current treatment of choice. Overall 5-year survival rates range from 9% to 28%, and reach as high as 24-43% in R0 resections. Five-year survival rates in the very limited experience with LTx in hilar cholangiocarcinoma are not dramatically worse than those after resection. However, hilar cholangiocarcinoma is not at present an accepted indication for LTx given both the good results of LTx for benign diseases and the dramatic organ shortage. When compared with the prognosis of other gastrointestinal tumours, these survival rates are encouraging in the setting of an otherwise unresectable malignancy. As such, and considering the fact that it may represent the only possibility for cure, the general exclusion of patients with cholangiocarcinomas as candidates for LTx does not seem to be justified. Furthermore, recent advances in multimodal tumour therapy seem to be most promising in combination with LTx. Prospective studies are required to elucidate the influence of better patient selection and the role of multimodal treatments on the outcome of LTx in hilar cholangiocarcinoma. If the encouraging data achieved with neoadjuvant therapy prior to LTx are confirmed by further studies, we foresee that renewed interest in LTx for hilar cholangiocarcinoma could arise. | 2005 | HPB : the official journal of the International Hepato Pancreato Biliary Association | No DOI | [
{
"LastName": "Lang",
"FirstName": "Hauke",
"Affiliation": "Klinik für Allgemein- und Transplantationschirurgie, Universitätsklinikum Essen, Essen, Germany. hauke.lang@uni-essen.de"
},
{
"LastName": "Sotiropoulos",
"FirstName": "Georgios C",
"Affiliation": ""
},
{
"LastName": "Kaiser",
"FirstName": "Gernot M",
"Affiliation": ""
},
{
"LastName": "Molmenti",
"FirstName": "Ernesto P",
"Affiliation": ""
},
{
"LastName": "Malagó",
"FirstName": "Massimo",
"Affiliation": ""
},
{
"LastName": "Broelsch",
"FirstName": "Christoph E",
"Affiliation": ""
}
] | No |
31184227 | Ras-ERK signalling represses H1.4 phosphorylation at serine 36 to promote non-small-cell lung carcinoma cells growth and migration. | Recent papers suggest that oncogenic Ras participate in regulating tumour cells proliferation and metastasis. This work linked Ras with H1.4 modification in non-small-cell lung carcinoma (NSCLC), to better understand the oncogenic effects of Ras. A plasmid for expressing Ras mutated at G13D and T35S was transfected into NCI-H2126 and A549 cells. Phosphorylation of H1.4S36 was determined by immunoblotting. Effects of phosphorylation of H1.4 at serine (S) 36 (H1.4S36ph) on NCI-H2126 and A549 cells were tested by MTT assay, soft-agar colony formation assay, flow cytometry and transwell assay. Chromatin-immunoprecipitation (ChIP) and RT-qPCR were conducted to measure the effects of H1.4S36ph on Ras downstream genes. The catalyzing enzymes participate in H1.4S36 phosphorylation were further studied. We found that Ras-ERK signalling repressed the phosphorylation of H1.4 at S36. H1.4S36ph functioned as a tumour suppressor, as its overexpression repressed NCI-H2126 and A549 cells viability, colony formation, S-phase arrest, migration and invasion. H1.4S36ph was able to mediate the transcription of Ras downstream genes. Ras-ERK signalling repressed H1.4S36ph through degradation of PKA, and the degradation was mediated by MDM2. In conclusion, Ras-ERK signalling repressed H1.4 phosphorylation at S36 to participate in NSCLC cells growth, migration and invasion. Ras-ERK signalling repressed H1.4S36ph through MDM2-dependent degradation of PKA. This study provides a novel explanation for Ras-ERK's tumour-promoting function. Highlights: H1.4S36 phosphorylation is repressed by Ras-ERK activation; H1.4S36ph inhibits the phenotype of NSCLC cells; H1.4S36ph regulates the transcription of Ras downstream genes; Ras-ERK represses H1.4S36ph by MDM2-dependent degradation of PKA. | 2019 Dec | Artificial cells, nanomedicine, and biotechnology | No DOI | [
{
"LastName": "Shi",
"FirstName": "Shaomin",
"Affiliation": "a Department of Respiratory, China-Japan Union Hospital of Jilin University , Changchun , China."
},
{
"LastName": "Zhang",
"FirstName": "Jingzhe",
"Affiliation": "b Department of Orthopedics, China-Japan Union Hospital of Jilin University , Changchun , China."
},
{
"LastName": "Liu",
"FirstName": "Meihan",
"Affiliation": "c Department of Ultrasound, China-Japan Union Hospital of Jilin University , Changchun , China."
},
{
"LastName": "Dong",
"FirstName": "Hang",
"Affiliation": "b Department of Orthopedics, China-Japan Union Hospital of Jilin University , Changchun , China."
},
{
"LastName": "Li",
"FirstName": "Ning",
"Affiliation": "a Department of Respiratory, China-Japan Union Hospital of Jilin University , Changchun , China."
}
] | Yes |
38609272 | Quantifying payloads of antibody‒drug conjugates using a postcolumn infused-internal standard strategy with LC‒MS. | Antibody‒drug conjugates (ADCs) are innovative biopharmaceutics consisting of a monoclonal antibody, linkers, and cytotoxic payloads. Monitoring circulating payload concentrations has the potential to identify ADC toxicity; however, accurate quantification faces challenges, including low plasma concentrations, severe matrix effects, and the absence of stable isotope-labeled internal standards (SIL-IS) for payloads and their derivatives. Previous studies used structural analogs as internal standards, but different retention times between structural analogs and target analytes may hinder effective matrix correction. Therefore, a more flexible approach is required for precise payload quantification. We developed an LC‒MS/MS method incorporating a postcolumn-infused internal standard (PCI-IS) strategy for quantifying payloads and their derivatives of trastuzumab emtansine, trastuzumab deruxtecan, and sacituzumab govitecan, including DM1, MCC-DM1, DXd, SN-38, and SN-38G. Structural analogs (maytansine, Lys-MCC-DM1, and exatecan) were selected as PCI-IS candidates, and their accuracy performance was evaluated based on the percentage of samples within 80%-120% quantification accuracy. Compared to the approach without PCI-IS correction, exatecan enhanced the accuracy performance from 30-40%-100% for SN-38 and DXd, while maytansine and Lys-MCC-DM1 showed comparable accuracy for DM1 and MCC-DM1. This validated PCI-IS analytical method showed superior normalization of matrix effect in all analytes compared to the conventional internal standard approach. The clinical application of this approach showed pronounced differences in DXd and SN-38 concentrations before and after PCI-IS correction. Moreover, only DXd concentrations after PCI-IS correction were significantly higher in patients with thrombocytopenia (p = 0.037). This approach effectively addressed the issue of unavailability of SIL-IS for novel ADC payloads and provided more accurate quantification, potentially yielding more robust statistical outcomes for understanding the exposure-toxicity relationship in ADCs. It is anticipated that this PCI-IS strategy may be extrapolated to quantify payloads and derivatives in diverse ADCs, thereby providing invaluable insights into drug toxicity and fortifying patient safety in ADC usage. | 2024 May 15 | Analytica chimica acta | No DOI | [
{
"LastName": "Cheng",
"FirstName": "Chih-Ning",
"Affiliation": "School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan."
},
{
"LastName": "Liao",
"FirstName": "Hsiao-Wei",
"Affiliation": "Department of Pharmacy, National Yang Ming Chiao Tung University, Taipei, Taiwan."
},
{
"LastName": "Lin",
"FirstName": "Ching-Hung",
"Affiliation": "Department of Oncology, National Taiwan University Hospital, Taipei, Taiwan; Department of Medical Oncology, National Taiwan University Hospital, Cancer Center Branch, Taipei, Taiwan."
},
{
"LastName": "Chang",
"FirstName": "Wen-Chi",
"Affiliation": "Department of Medical Research, National Taiwan University Hospital, Taipei, Taiwan."
},
{
"LastName": "Chen",
"FirstName": "I-Chun",
"Affiliation": "Department of Oncology, National Taiwan University Hospital, Taipei, Taiwan; Department of Medical Oncology, National Taiwan University Hospital, Cancer Center Branch, Taipei, Taiwan."
},
{
"LastName": "Lu",
"FirstName": "Yen-Shen",
"Affiliation": "Department of Oncology, National Taiwan University Hospital, Taipei, Taiwan."
},
{
"LastName": "Kuo",
"FirstName": "Ching-Hua",
"Affiliation": "School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan; The Metabolomics Core Laboratory, Centers of Genomic and Precision Medicine, National Taiwan University, Taiwan; Department of Pharmacy, National Taiwan University Hospital, Taipei, Taiwan. Electronic address: kuoch@ntu.edu.tw."
}
] | No |
37522061 | Aggressive intraosseous lipoma of the scapula: A case report. | The details of the pathogenesis of intraosseous lipomas are not fully elucidated, although most cases do not require surgical treatment. The present report describes the case of a 79-year-old female patient diagnosed with intracapsular lipoma who also exhibited an extraosseous extension. Chest computed tomography revealed an abnormal shadow or a mass in the right scapula and destruction of the glenoid bone. Magnetic resonance imaging revealed a high-intensity mass on T1-weighted and T2-weighted images in the same area. Marginal resection of the mass was performed. The histopathology confirmed that the mass was a lipoma. No postoperative recurrence was observed. Oncologists must be aware that lipoma arising within the scapula may extend outside the bone. | 2023 Aug | Experimental and therapeutic medicine | No DOI | [
{
"LastName": "Oka",
"FirstName": "Naohiro",
"Affiliation": "Department of Orthopedic Surgery, Kushimoto Municipality Hospital, Kushimoto-Cho, Wakayama 649-3510, Japan."
},
{
"LastName": "Hashimoto",
"FirstName": "Kazuhiko",
"Affiliation": "Department of Orthopedic Surgery, Kushimoto Municipality Hospital, Kushimoto-Cho, Wakayama 649-3510, Japan."
},
{
"LastName": "Shinyashiki",
"FirstName": "Yu",
"Affiliation": "Department of Orthopedic Surgery, Kushimoto Municipality Hospital, Kushimoto-Cho, Wakayama 649-3510, Japan."
},
{
"LastName": "Nishimura",
"FirstName": "Shunji",
"Affiliation": "Department of Orthopedic Surgery, Kindai University Hospital, Osaka-Sayama, Osaka 589-8511, Japan."
},
{
"LastName": "Akagi",
"FirstName": "Masao",
"Affiliation": "Department of Orthopedic Surgery, Kindai University Hospital, Osaka-Sayama, Osaka 589-8511, Japan."
}
] | No |
30276125 | The Death of the Kennedy Terminal Ulcer. | The concept of the Kennedy Terminal Ulcer (KTU) has been ubiquitous in attempting to explain the development of pressure based tissue injuries in patients with actual or presumed terminal conditions. The concept is problematic in that it uses factors other than pressure to explain the development and progression of pressure based tissue injuries, specifically the presence of a terminal condition. Based on the most current understanding of how pressure based tissue injuries develop and progress, the concept of The Kennedy Terminal Ulcer appears to be without physiologic basis and based solely on observation. Since systemic factors affect all tissues with relative equality, the development of a single locus of injury must logically be based on a single locus of cause and affect. The presumption that a single locus of injury will develop in an arbitrary location based on a systemic set of factors is untenable. A new concept called Miller Pressure Equivalent Injuries is proposed to refute the concept of a single pressure based tissue injury developing based solely on terminal systemic factors and why these previously presumed terminal condition associated pressure based injuries occur. | 2016 | The journal of the American College of Clinical Wound Specialists | No DOI | [
{
"LastName": "Miller",
"FirstName": "Michael S",
"Affiliation": "CEO and Medical Director - Miller Care Group, Indianapolis, IN, USA."
}
] | No |
38235305 | Applying a participatory systems and value approach in a transdisciplinary exercise: on assessing the impact of training and education initiatives. | Participatory systems approaches are readily used in multi- and inter-disciplinary exploration of shared processes, but are less-commonly applied in trans-disciplinary efforts eliciting principles that generalise across contexts. The authors were charged with developing a transdisciplinary framework for prospectively or retrospectively assessing initiatives to improve education and training within a multifaceted organisation. A common System Impact Model (SIM) was developed in a series of workshops involving thirty participants from different disciplines, clinical specialisms, and organisations. The model provided a greater understanding of the interrelationships between factors influencing the benefits of education and training and development as seen from various stakeholder perspectives. It was used to create a system for assessing the impact of initiatives on service-users/patients, trainees, and organisations. It was shown to enable a range of participants to connect on common challenges, to maximise cross-, multi-, and inter-disciplinary learning, and to uncover new strategies for delivering value, as system designers. | 2023 | Health systems (Basingstoke, England) | No DOI | [
{
"LastName": "Akinluyi",
"FirstName": "Emmanuel A",
"Affiliation": "Medical Physics Department, Guy's & St Thomas' NHS Foundation Trust, London, UK."
},
{
"LastName": "Greenough",
"FirstName": "Anne",
"Affiliation": "Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK."
},
{
"LastName": "Ison",
"FirstName": "Keith",
"Affiliation": "Medical Physics Department, Guy's & St Thomas' NHS Foundation Trust, London, UK."
},
{
"LastName": "Clarkson",
"FirstName": "P John",
"Affiliation": "Engineering Design Centre, Department of Engineering, University of Cambridge, Cambridge, UK."
}
] | No |
35723795 | LncRNA UCA1 epigenetically suppresses APAF1 expression to mediate the protective effect of sevoflurane against myocardial ischemia-reperfusion injury. | Myocardial ischemia-reperfusion injury (MI/RI) is a leading cause of death globally. Whereas some long noncoding RNAs (lncRNAs) are known to participate in the progression of MI/RI, the role of urothelial carcinoma associated 1 (UCA1) in conjunction with sevoflurane treatment remains largely unknown. H9C2 cardiomyocytes were subjected to hypoxia/reoxygenation (H/R) to establish an in vitro MI/RI model, and sevoflurane was then added. Cell viability, apoptosis, SOD activity, and MDA levels were measured. Levels of inflammatory cytokines and methylation of apoptosis protease-activating factor 1 (APAF1) were determined. Interactions among lncRNA UCA1, enhancer of zeste homologue 2 (EZH2), DNA methyltransferase-1 (DNMT1), and APAF1 were analyzed. After H/R treatment, the viability of H9C2 cardiomyocytes decreased and apoptosis rate, oxidative stress factor levels, inflammatory cytokine levels, and apoptosis-related protein levels all increased. Sevoflurane treatment reversed these changes. LncRNA UCA1 knockdown attenuated the therapeutic effect of sevoflurane on H/R-treated cardiomyocytes, and silencing of APAF1 reversed this role of UCA1 knockdown. Moreover, lncRNA UCA1 recruited DNMT1 through EZH2, thus promoting methylation of the APAF1 promoter region. LncRNA UCA1 recruits DNMT1 to promote methylation of the APAF1 promoter through EZH2, thus strengthening the protective effect of sevoflurane on H/R-induced cardiomyocyte injury. | 2022 Oct | Functional & integrative genomics | No DOI | [
{
"LastName": "Jin",
"FirstName": "Guanjun",
"Affiliation": "Department of Anesthesiology, Ningbo First Hospital, No. 90, Xianxue Street, Haishu District, Ningbo, 315010, Zhejiang, China."
},
{
"LastName": "Zheng",
"FirstName": "Jungang",
"Affiliation": "Department of Anesthesiology, Ningbo First Hospital, No. 90, Xianxue Street, Haishu District, Ningbo, 315010, Zhejiang, China."
},
{
"LastName": "Zhang",
"FirstName": "Yiwei",
"Affiliation": "Department of Anesthesiology, Ningbo First Hospital, No. 90, Xianxue Street, Haishu District, Ningbo, 315010, Zhejiang, China."
},
{
"LastName": "Yang",
"FirstName": "Zhaodong",
"Affiliation": "Department of Anesthesiology, Ningbo First Hospital, No. 90, Xianxue Street, Haishu District, Ningbo, 315010, Zhejiang, China."
},
{
"LastName": "Chen",
"FirstName": "Yijun",
"Affiliation": "Department of Anesthesiology, Ningbo First Hospital, No. 90, Xianxue Street, Haishu District, Ningbo, 315010, Zhejiang, China. Chenyijun5246@163.com."
},
{
"LastName": "Huang",
"FirstName": "Changshun",
"Affiliation": "Department of Anesthesiology, Ningbo First Hospital, No. 90, Xianxue Street, Haishu District, Ningbo, 315010, Zhejiang, China. caixi51@163.com."
}
] | No |
37315729 | Protease-Activated Receptor 2 (PAR2) Expressed in Sensory Neurons Contributes to Signs of Pain and Neuropathy in Paclitaxel Treated Mice. | Chemotherapy-induced peripheral neuropathy (CIPN) is a common, dose-limiting side effect of cancer therapy. Protease-activated receptor 2 (PAR2) is implicated in a variety of pathologies, including CIPN. In this study, we demonstrate the role of PAR2 expressed in sensory neurons in a paclitaxel (PTX)-induced model of CIPN in mice. PAR2 knockout/wildtype (WT) mice and mice with PAR2 ablated in sensory neurons were treated with PTX administered via intraperitoneal injection. In vivo behavioral studies were done in mice using von Frey filaments and the Mouse Grimace Scale. We then examined immunohistochemical staining of dorsal root ganglion (DRG) and hind paw skin samples from CIPN mice to measure satellite cell gliosis and intra-epidermal nerve fiber (IENF) density. The pharmacological reversal of CIPN pain was tested with the PAR2 antagonist C781. Mechanical allodynia caused by PTX treatment was alleviated in PAR2 knockout mice of both sexes. In the PAR2 sensory neuronal conditional knockout (cKO) mice, both mechanical allodynia and facial grimacing were attenuated in mice of both sexes. In the DRG of the PTX-treated PAR2 cKO mice, satellite glial cell activation was reduced compared to control mice. IENF density analysis of the skin showed that the PTX-treated control mice had a reduction in nerve fiber density while the PAR2 cKO mice had a comparable skin innervation as the vehicle-treated animals. Similar results were seen with satellite cell gliosis in the DRG, where gliosis induced by PTX was absent in PAR cKO mice. Finally, C781 was able to transiently reverse established PTX-evoked mechanical allodynia. PERSPECTIVE: Our work demonstrates that PAR2 expressed in sensory neurons plays a key role in PTX-induced mechanical allodynia, spontaneous pain, and signs of neuropathy, suggesting PAR2 as a possible therapeutic target in multiple aspects of PTX CIPN. | 2023 Nov | The journal of pain | No DOI | [
{
"LastName": "Kume",
"FirstName": "Moeno",
"Affiliation": "Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, Texas."
},
{
"LastName": "Ahmad",
"FirstName": "Ayesha",
"Affiliation": "Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, Texas."
},
{
"LastName": "DeFea",
"FirstName": "Kathryn A",
"Affiliation": "PARMedics Incorporated, Temecula, CA."
},
{
"LastName": "Vagner",
"FirstName": "Josef",
"Affiliation": "Bio5 Research Institute, University of Arizona, Tucson, Arizona."
},
{
"LastName": "Dussor",
"FirstName": "Gregory",
"Affiliation": "Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, Texas."
},
{
"LastName": "Boitano",
"FirstName": "Scott",
"Affiliation": "Bio5 Research Institute, University of Arizona, Tucson, Arizona; Asthma and Airway Disease Research Center, University of Arizona Health Sciences, Tucson, Arizona; Department of Physiology, University of Arizona Health Sciences, Tucson, Arizona."
},
{
"LastName": "Price",
"FirstName": "Theodore J",
"Affiliation": "Department of Neuroscience and Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, Texas."
}
] | No |
33125145 | High mobility group box 1 induces bone pain associated with bone invasion in a mouse model of advanced head and neck cancer. | Advanced head and neck cancer (HNC) can invade facial bone and cause bone pain, thus posing a significant challenge to the quality of life of patients presenting with advanced HNC. The present study was designed to investigate HNC bone pain (HNC‑BP) in an intratibial mouse xenograft model that utilized an HNC cell line (SAS cells). These mice develop HNC‑BP that is associated with an expression of phosphorylated ERK1/2 (pERK1/2), which is a molecular indicator of neuron excitation in dorsal root ganglia (DRG) sensory neurons. Our experiments demonstrated that the inhibition of high mobility group box 1 (HMGB1) by short hairpin (shRNA) transduction, HMGB1 neutralizing antibody, and HMGB1 receptor antagonist suppressed the HNC‑BP and the pERK1/2 expression in DRG. It was also observed that HNC‑derived HMGB1 increased the expression of the acid‑sensing nociceptor, transient receptor potential vanilloid 1 (TRPV1), which is a major cause of osteoclastic HNC‑BP in DRG. Collectively, our results demonstrated that HMGB1 originating in HNC evokes HNC‑BP via direct HMGB1 signaling and hypersensitization for the acid environment in sensory neurons. | 2020 Dec | Oncology reports | No DOI | [
{
"LastName": "Nakamura",
"FirstName": "Tomoya",
"Affiliation": "Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700‑8525, Japan."
},
{
"LastName": "Okui",
"FirstName": "Tatsuo",
"Affiliation": "Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700‑8525, Japan."
},
{
"LastName": "Hasegawa",
"FirstName": "Kazuaki",
"Affiliation": "Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700‑8525, Japan."
},
{
"LastName": "Ryumon",
"FirstName": "Shoji",
"Affiliation": "Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700‑8525, Japan."
},
{
"LastName": "Ibaragi",
"FirstName": "Soichiro",
"Affiliation": "Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700‑8525, Japan."
},
{
"LastName": "Ono",
"FirstName": "Kisho",
"Affiliation": "Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700‑8525, Japan."
},
{
"LastName": "Kunisada",
"FirstName": "Yuki",
"Affiliation": "Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700‑8525, Japan."
},
{
"LastName": "Obata",
"FirstName": "Kyoichi",
"Affiliation": "Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700‑8525, Japan."
},
{
"LastName": "Masui",
"FirstName": "Masanori",
"Affiliation": "Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700‑8525, Japan."
},
{
"LastName": "Shimo",
"FirstName": "Tsuyoshi",
"Affiliation": "Division of Reconstructive Surgery for Oral and Maxillofacial Region, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061‑0293, Japan."
},
{
"LastName": "Sasaki",
"FirstName": "Akira",
"Affiliation": "Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama 700‑8525, Japan."
}
] | No |
38997398 | Mimicking classical noise in ion channels by quantum decoherence. | The mechanism of selectivity in ion channels is still an open question in biology. Recent studies suggest that the selectivity filter may exhibit quantum coherence, which could help explain how ions are selected and conducted. However, environmental noise causes decoherence and loss of quantum effects. It is hoped that the effect of classical noise on ion channels can be modeled using the framework provided by quantum decoherence theory. In this paper, the behavior of the ion channel system was simulated using two models: the Spin-Boson model and the stochastic Hamiltonian model under classical noise. Additionally, using a different approach, the system's evolution was modeled as a two-level Spin-Boson model with tunneling, interacting with a bath of harmonic oscillators, based on decoherence theory. We investigated under what conditions the decoherence model approaches and deviates from the noise model. Specifically, we examined Gaussian noise and Ornstein-Uhlenbeck noise in our model. Gaussian noise shows a very good agreement with the decoherence model. By examining the results, it was found that the Spin-Boson model at a high hopping rate of potassium ions can simulate the behavior of the system in the classical noise approach for Gaussian noise. | 2024 Jul 12 | Scientific reports | No DOI | [
{
"LastName": "Seifi",
"FirstName": "Mina",
"Affiliation": "Research Group on Foundations of Quantum Theory and Information, Department of Chemistry, Sharif University of Technology, P.O. Box 11365-9516, Tehran, Iran."
},
{
"LastName": "Soltanmanesh",
"FirstName": "Ali",
"Affiliation": "Research Group on Foundations of Quantum Theory and Information, Department of Chemistry, Sharif University of Technology, P.O. Box 11365-9516, Tehran, Iran."
},
{
"LastName": "Shafiee",
"FirstName": "Afshin",
"Affiliation": "Research Group on Foundations of Quantum Theory and Information, Department of Chemistry, Sharif University of Technology, P.O. Box 11365-9516, Tehran, Iran. shafiee@sharif.edu."
}
] | No |
22347651 | Exercise-induced right ventricular outflow tract tachycardia in a patient with isolated left ventricular noncompaction. | Isolated left ventricular noncompaction is a hereditary cardiomyopathy in which a variety of supraventricular and ventricular arrhythmias could be observed. We report a patient with exercise-induced ventricular tachycardia with left bundle branch block morphology that had characteristics of an idiopathic ventricular tachycardia who was subsequently diagnosed as left ventricular noncompaction. Successful remission of arrhythmia was ensured after the introduction of oral beta-blocker therapy. | 2011 | ISRN cardiology | No DOI | [
{
"LastName": "Güvenç",
"FirstName": "Tolga Sinan",
"Affiliation": "Kafkas University Faculty of Medicine, Department of Cardiology, 5 Kars, Turkey."
},
{
"LastName": "Ilhan",
"FirstName": "Erkan",
"Affiliation": ""
},
{
"LastName": "Alper",
"FirstName": "Ahmet Taha",
"Affiliation": ""
},
{
"LastName": "Eren",
"FirstName": "Mehmet",
"Affiliation": ""
}
] | No |
36960858 | High‑risk pathological subtype associated FAM83A‑AS1 promotes malignancy and glycolysis of lung adenocarcinoma via miR‑202‑3p/HK2 axis. | According to the diverse cellular morphology, lung adenocarcinoma (LUAD) was classified into five pathological subtypes, referred to as follows: High‑risk group (micropapillary and solid), intermediate‑risk group (acinar and papillary) and low‑risk group (epidic). Nevertheless, little is known about the biological function of long non‑coding RNA (lncRNA) in the molecular determination of LUAD histologic patterns. Screening the transcriptional expression data from TCGA‑LUAD, the differentially expressed lncRNA across the divergent pathological subtypes were explored. Pan‑cancer analysis revealed the characteristic of FAM83A‑AS1, which was also confirmed in the LUAD tissues. The function of FAM83A‑AS1 was uncovered through the in vitro assays. RNA immunoprecipitation and dual‑luciferase reporter assays were performed to explore the molecular mechanisms of FAM83A‑AS1. In the present study, it was identified that the expression of FAM83A‑AS1 was increased from the low‑risk group to the high, which was associated with a poorer prognosis and higher risk of recurrence. Pan‑cancer analysis revealed that FAM83A‑AS1 was positively correlated with high tumor mutational burden. Additionally, FAM83A‑AS1 promoted cell migration, invasion and growth of LUAD cancer cells. Mechanistically, FAM83A‑AS1 sponged miR‑202‑3p to regulate the expression of hexokinase II (HK2) in post‑transcription, which facilitated the malignancy and glycolysis. The present study uncovered the biological roles of FAM83A‑AS1/miR‑202‑3p/HK2 axis in regulating malignancy and glycolysis of LUAD, which provided novel avenues to addressing the determination of histologic patterns. | 2023 May | Oncology reports | No DOI | [
{
"LastName": "Xiong",
"FirstName": "Xinkui",
"Affiliation": "Department of Thoracic Surgery, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaiyin, Huaian, Jiangsu 223001, P.R. China."
},
{
"LastName": "Zhang",
"FirstName": "Lei",
"Affiliation": "Department of Medical Oncology, Xuzhou Central Hospital, Quanshan, Xuzhou 221000, P.R. China."
},
{
"LastName": "Zang",
"FirstName": "Bao",
"Affiliation": "Department of Thoracic Surgery, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaiyin, Huaian, Jiangsu 223001, P.R. China."
},
{
"LastName": "Xu",
"FirstName": "Dafu",
"Affiliation": "Department of Thoracic Surgery, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaiyin, Huaian, Jiangsu 223001, P.R. China."
},
{
"LastName": "Chen",
"FirstName": "Chen",
"Affiliation": "Department of Thoracic Surgery, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaiyin, Huaian, Jiangsu 223001, P.R. China."
},
{
"LastName": "Dong",
"FirstName": "Gaochao",
"Affiliation": "Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Nanjing, Jiangsu 210000, P.R. China."
},
{
"LastName": "Xia",
"FirstName": "Wenjie",
"Affiliation": "Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Nanjing, Jiangsu 210000, P.R. China."
},
{
"LastName": "Wu",
"FirstName": "Yanhu",
"Affiliation": "Department of Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China."
}
] | No |
35938715 | Sex-dependent role of Pannexin 1 in regulating skeletal muscle and satellite cell function. | The development and regeneration of skeletal muscle are mediated by satellite cells (SCs), which ensure the efficient formation of myofibers while repopulating the niche that allows muscle repair following injuries. Pannexin 1 (Panx1) channels are expressed in SCs and their levels increase during differentiation in vitro, as well as during skeletal muscle development and regeneration in vivo. Panx1 has recently been shown to regulate muscle regeneration by promoting bleb-based myoblast migration and fusion. While skeletal muscle is largely influenced in a sex-specific way, the sex-dependent roles of Panx1 in regulating skeletal muscle and SC function remain to be investigated. Here, using global Panx1 knockout (KO) mice, we demonstrate that Panx1 loss reduces muscle fiber size and strength, decreases SC number, and alters early SC differentiation and myoblast fusion in male, but not in female mice. Interestingly, while both male and female Panx1 KO mice display an increase in the number of regenerating fibers following acute injury, the newly formed fibers in male Panx1 KO mice are smaller. Overall, our results demonstrate that Panx1 plays a significant role in regulating muscle development, regeneration, and SC number and function in male mice and reveal distinct sex-dependent functions of Panx1 in skeletal muscle. | 2022 Oct | Journal of cellular physiology | No DOI | [
{
"LastName": "Freeman",
"FirstName": "Emily",
"Affiliation": "Molecular Biomedicine Program, Children's Hospital of Eastern Ontario Research Institute, Ottawa, Ontario, Canada."
},
{
"LastName": "Langlois",
"FirstName": "Stéphanie",
"Affiliation": "Molecular Biomedicine Program, Children's Hospital of Eastern Ontario Research Institute, Ottawa, Ontario, Canada."
},
{
"LastName": "Scott",
"FirstName": "Kaylee",
"Affiliation": "Molecular Biomedicine Program, Children's Hospital of Eastern Ontario Research Institute, Ottawa, Ontario, Canada."
},
{
"LastName": "Ravel-Chapuis",
"FirstName": "Aymeric",
"Affiliation": "Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada."
},
{
"LastName": "Jasmin",
"FirstName": "Bernard J",
"Affiliation": "Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada."
},
{
"LastName": "Cowan",
"FirstName": "Kyle N",
"Affiliation": "Molecular Biomedicine Program, Children's Hospital of Eastern Ontario Research Institute, Ottawa, Ontario, Canada."
}
] | No |
37972689 | COPD Exposed to Air Pollution: A Path to Understand and Protect a Susceptible Population. | Air pollution poses a risk to the respiratory health of individuals with COPD. Long- and short-term exposures to higher levels of particulate-rich air pollution are associated with increased COPD exacerbation, hospitalization, and mortality, collectively implicating air pollution as a cause of adverse COPD-related outcomes. This review summarizes the evidence for COPD as a phenotype that confers susceptibility for adverse health outcomes in the face of common air pollution. We highlight how typical contributors to compromised urban air quality, including that from traffic, wildfire smoke, and indoor biomass combustion, adversely affect the COPD patient population. Evidence underscoring the burden of ongoing air pollution exposure on patients with COPD is discussed. We then detail the detrimental impact of that exposure on COPD pathophysiology, which in turn increases the patient's susceptibility. We specifically propose that indoor air is a particularly rational target for increased monitoring and remediation to protect patients with COPD. Because COPD is a heterogeneous disease with several endotypes, future intervention studies need to better include control populations, to highlight COPD-specific risks and identify subpopulations within patients with COPD who will benefit the most from improved indoor air quality. Regulatory efforts must continue to broadly lower emission standards to protect this susceptible population from the negative health impacts of air pollution. | 2024 Apr | Chest | No DOI | [
{
"LastName": "Ryu",
"FirstName": "Min Hyung",
"Affiliation": "Air Pollution Exposure Laboratory, Division of Respiratory Medicine, The University of British Columbia, Vancouver, BC, Canada."
},
{
"LastName": "Murphy",
"FirstName": "Shane",
"Affiliation": "Air Pollution Exposure Laboratory, Division of Respiratory Medicine, The University of British Columbia, Vancouver, BC, Canada."
},
{
"LastName": "Hinkley",
"FirstName": "Madison",
"Affiliation": "Air Pollution Exposure Laboratory, Division of Respiratory Medicine, The University of British Columbia, Vancouver, BC, Canada."
},
{
"LastName": "Carlsten",
"FirstName": "Chris",
"Affiliation": "Air Pollution Exposure Laboratory, Division of Respiratory Medicine, The University of British Columbia, Vancouver, BC, Canada; Legacy for Airway Health and Centre for Lung Health, Vancouver Coastal Health Research Institute, Vancouver, BC, Canada. Electronic address: carlsten@mail.ubc.ca."
}
] | No |
35115946 | Traditional Chinese Medicine Targeting Heat Shock Proteins as Therapeutic Strategy for Heart Failure. | Heart failure (HF) is the terminal stage of multifarious heart diseases and is responsible for high hospitalization rates and mortality. Pathophysiological mechanisms of HF include cardiac hypertrophy, remodeling and fibrosis resulting from cell death, inflammation and oxidative stress. Heat shock proteins (HSPs) can ameliorate folding of proteins, maintain protein structure and stability upon stress, protect the heart from cardiac dysfunction and ameliorate apoptosis. Traditional Chinese medicine (TCM) regulates expression of HSPs and has beneficial therapeutic effect in HF. In this review, we summarized the function of HSPs in HF and the role of TCM in regulating expression of HSPs. Studying the regulation of HSPs by TCM will provide novel ideas for the study of the mechanism and treatment of HF. | 2021 | Frontiers in pharmacology | No DOI | [
{
"LastName": "Wang",
"FirstName": "Yanchun",
"Affiliation": "Shenyang the Tenth People's Hospital, Shenyang, China."
},
{
"LastName": "Wu",
"FirstName": "Junxuan",
"Affiliation": "Shunde Hospital of Guangzhou University of Chinese Medicine, Foshan, China."
},
{
"LastName": "Wang",
"FirstName": "Dawei",
"Affiliation": "Shunde Hospital of Guangzhou University of Chinese Medicine, Foshan, China."
},
{
"LastName": "Yang",
"FirstName": "Rongyuan",
"Affiliation": "The Second Clinical School of Medicine, Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine-Zhuhai Hospital, Zhuhai, China."
},
{
"LastName": "Liu",
"FirstName": "Qing",
"Affiliation": "The Second Clinical School of Medicine, Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine-Zhuhai Hospital, Zhuhai, China."
}
] | No |
38352628 | Pleurotus ostreatus is a promising candidate of an edible 3D printing ink: Investigation of printability and characterization. | The 3D printing (3DP) technology shows great potential in the food industry, but the development of edible ink is currently insufficient. Pleurotus ostreatus (P. ostreatus) emerges as a novel promising candidate. In this study, a mixed ink was obtained by incorporating butter into P. ostreatus. The effects of different ratios of P. ostreatus and butter, as well as the influence of ink steaming were investigated on 3D printed products. The results indicated that all inks of the P. ostreatus system exhibited positive shear-thinning behavior, and the system maintained stable intermolecular hydrogen bonding when P. ostreatus powder concentration was 40 % (w/v). Furthermore, the L* value of the system was elevated for butter adding. The system with steaming exhibited superior stabilized molecular structure compared to the native system, particularly with a steaming duration of 5 min, showcasing its outstanding supporting capacity. This study suggests that P. ostreatus is a promising candidate in 3DP for the development of an edible ink that promotes innovation and nutritional food. | 2024 | Current research in food science | No DOI | [
{
"LastName": "Liu",
"FirstName": "Rui",
"Affiliation": "College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, 210023, China."
},
{
"LastName": "Hu",
"FirstName": "Qiuhui",
"Affiliation": "College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, 210023, China."
},
{
"LastName": "Ma",
"FirstName": "Gaoxing",
"Affiliation": "College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, 210023, China."
},
{
"LastName": "Pei",
"FirstName": "Fei",
"Affiliation": "College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, 210023, China."
},
{
"LastName": "Zhao",
"FirstName": "Liyan",
"Affiliation": "College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China."
},
{
"LastName": "Ma",
"FirstName": "Ning",
"Affiliation": "College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, 210023, China."
},
{
"LastName": "Yang",
"FirstName": "Fan",
"Affiliation": "College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, 210023, China."
},
{
"LastName": "Liu",
"FirstName": "Xiao",
"Affiliation": "College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China."
},
{
"LastName": "Su",
"FirstName": "Anxiang",
"Affiliation": "College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, 210023, China."
}
] | No |
36718218 | Loss of Lactate/Proton Monocarboxylate Transporter 4 Induces Ferroptosis via the AMPK/ACC Pathway and Inhibition of Autophagy on Human Bladder Cancer 5637 Cell Line. | Ferroptosis and autophagy have an important role in the occurrence and development of cancer, and lactate in cells and microenvironment is one of the influencing factors of ferroptosis and autophagy. The lactate/proton monocarboxylate transporter 4 (MCT4), which is expressed in the cell membrane, regulates the transport of intracellular lactic acid and lactate. The knockout of MCT4 can affect intracellular and extracellular lactic acid levels, thereby affecting the growth, proliferation, and metastasis of tumor cells via regulation of the oxidative stress in cells. However, whether MCT4 affects ferroptosis and autophagy in bladder cancer cells remains unclear. Colony formation assay and bladder cancer xenograft animal model were used to assess the effect of MCT4 on the growth in 5637 cells. Reactive oxygen species (ROS) assay, lipid ROS assay, lipid peroxidation assay (MDA), and transmission electron microscopy were performed to assess the level of lipid peroxidation in 5637 cells. RNA-sequence, RT-PCR, and Western Blot were used to analyze the mechanism of MCT4 of ferroptosis and autophagy. AdPlus-mCherry-GFP-LC3B reporter system was used to detect the effect of MCT4 on autophagy in 5637 cells, and the effect of knockdown of MCT4 on apoptosis was analyzed by flow cytometry. The mRNA level of MCT4 was significantly upregulated in patients with bladder cancer, which was associated with a poor prognosis. In vivo and in vitro studies demonstrated that knockdown of MCT4 could inhibit the proliferation of bladder cancer cells. Furthermore, knockdown of MCT4 led to the significant increase of ROS and MDA levels in 5637 cells and ferroptosis in 5637 cells induced by ferroptosis inducers including RSL3 (APExBIO) and erastin (APExBIO) via inhibition of AMPK-related proteins. Moreover, knockdown of MCT4 inhibited autophagy in 5637 cells, while siMCT4 promoted inhibition of autophagy by CQ (an autophagy inhibitor), which increased the level of apoptosis. This study confirmed that knockdown of MCT4 could affect oxidative stress and induce ferroptosis and inhibition of autophagy, thus suggesting that MCT4 may be a potential target for the treatment of bladder cancer. | 2023 | Journal of oncology | No DOI | [
{
"LastName": "Dong",
"FirstName": "Siqi",
"Affiliation": "Department of Andrology, The Second Hospital of Dalian Medical University, Dalian116000, China."
},
{
"LastName": "Zheng",
"FirstName": "Lei",
"Affiliation": "Department of Andrology, The Second Hospital of Dalian Medical University, Dalian116000, China."
},
{
"LastName": "Jiang",
"FirstName": "Tao",
"Affiliation": "Department of Andrology, The Second Hospital of Dalian Medical University, Dalian116000, China."
}
] | No |
26965986 | CYP2D6 Is Inducible by Endogenous and Exogenous Corticosteroids. | Although cytochrome P450 (CYP) 2D6 has been widely considered to be noninducible on the basis of human hepatocyte studies, in vivo data suggests that it is inducible by endo- and xenobiotics. Therefore, we investigated if the experimental conditions routinely used in human hepatocyte studies may be a confounding factor in the lack of in vitro induction of CYP2D6. Sandwich cultured human hepatocytes (SCHH) were preincubated with or without dexamethasone (100 nM) for 72 hours before incubation with 1μM endogenous (cortisol or corticosterone) or exogenous (dexamethasone or prednisolone) corticosteroids. At 72 hours, CYP2D6 mRNA, protein, and activity were quantified by real-time quantitative polymerase chain reaction, quantitative proteomics, and formation of dextrorphan from dextromethorphan, respectively. In the absence of supplemental dexamethasone, CYP2D6 activity, mRNA, and protein were significantly and robustly (>10-fold) induced by all four corticosteroids. However, this CYP2D6 induction was abolished in cells preincubated with supplemental dexamethasone. These data show, for the first time, that CYP2D6 is inducible in vitro but the routine presence of 100 nM dexamethasone in the culture medium masks this induction. Our cortisol data are in agreement with the clinical observation that CYP2D6 is inducible during the third trimester of pregnancy when the plasma concentrations of cortisol increase to ∼1μM. These findings, if confirmed in vivo, have implications for predicting CYP2D6-mediated drug-drug interactions and call for re-evaluation of regulatory guidelines on screening for CYP2D6 induction by xenobiotics. Our findings also suggest that cortisol may be a causative factor in the in vivo induction of CYP2D6 during pregnancy. | 2016 May | Drug metabolism and disposition: the biological fate of chemicals | No DOI | [
{
"LastName": "Farooq",
"FirstName": "Muhammad",
"Affiliation": "Department of Pharmaceutics, University of Washington, Seattle, Washington."
},
{
"LastName": "Kelly",
"FirstName": "Edward J",
"Affiliation": "Department of Pharmaceutics, University of Washington, Seattle, Washington."
},
{
"LastName": "Unadkat",
"FirstName": "Jashvant D",
"Affiliation": "Department of Pharmaceutics, University of Washington, Seattle, Washington jash@uw.edu."
}
] | Yes |
35531757 | Lidocaine vs. tramadol vs. placebo wound infiltration for post-cesarean section pain relief: a randomized controlled trial. | In low-income settings, postoperative pain relief could be challenging as a high patient/nurse ratio limits pain assessment and adequate analgesics administration. The multi-center prospective double-blinded parallel randomized controlled trial was done to compare lidocaine, tramadol, and placebo (saline) intraoperative wound infiltration to relieve post-cesarean section wound pain during the first 24 h. Ninety-nine cases were equally randomized into three groups, each containing 33 pregnant women undergoing cesarean section under general anesthesia. During operation, the wound was infiltrated subcutaneously with 20 mL of 2% lidocaine solution in the first group, 2 mg/kg tramadol in the second group, and saline in the third group. The primary outcome was to assess the postoperative pain at 2, 4, 6, 12, and 24 h by the Yes-No-Don't Know (YNDK) Scale, while the secondary outcome was to assess the need for further postoperative analgesia. Wound infiltration with lidocaine or tramadol was effective in pain relief, and both were superior to placebo. Wound infiltration with tramadol was superior to lidocaine in pain relief at 2 h and up to 24 h. Wound infiltration with tramadol has a more prolonged pain relief effect than lidocaine in post-cesarean section pain relief in patients performing cesarean section under general anesthesia lasting up to 24 h, and both are superior to placebo in pain relief. | 2022 Oct 26 | Journal of perinatal medicine | No DOI | [
{
"LastName": "Hussein",
"FirstName": "Ahmed",
"Affiliation": "Department of Obstetrics & Gynecology, October 6th University, Giza, Egypt."
},
{
"LastName": "Torky",
"FirstName": "Haitham",
"Affiliation": "Department of Obstetrics & Gynecology, October 6th University, Giza, Egypt."
},
{
"LastName": "Aly",
"FirstName": "Rania",
"Affiliation": "Department of Obstetrics & Gynecology, Al-Galaa Teaching Hospital, Cairo, Egypt."
},
{
"LastName": "Abdel-Rasheed",
"FirstName": "Mazen",
"Affiliation": "Department of Reproductive Health Research, National Research Centre, Giza, Egypt."
},
{
"LastName": "El-Baz",
"FirstName": "Ashraf",
"Affiliation": "Department of Obstetrics & Gynecology, Al-Galaa Teaching Hospital, Cairo, Egypt."
},
{
"LastName": "Mahmoud",
"FirstName": "Hossam",
"Affiliation": "Department of Obstetrics & Gynecology, Al-Galaa Teaching Hospital, Cairo, Egypt."
},
{
"LastName": "Sileem",
"FirstName": "Sileem",
"Affiliation": "Department of Obstetrics & Gynecology, Al-Azhar University, Assiut, Egypt."
},
{
"LastName": "Badawy",
"FirstName": "Mahmoud",
"Affiliation": "Department of Obstetrics & Gynecology, Al-Azhar University, Assiut, Egypt."
},
{
"LastName": "Sayd",
"FirstName": "Zainab",
"Affiliation": "Department of Obstetrics & Gynecology, Al-Azhar University, Assiut, Egypt."
},
{
"LastName": "Dief",
"FirstName": "Osama",
"Affiliation": "Department of Obstetrics & Gynecology, Al-Azhar University, Cairo, Egypt."
},
{
"LastName": "Elsadek",
"FirstName": "Ahmed",
"Affiliation": "Department of Obstetrics & Gynecology, Al-Azhar University, Cairo, Egypt."
},
{
"LastName": "Marie",
"FirstName": "Heba",
"Affiliation": "Department of Obstetrics & Gynecology, Cairo University, Cairo, Egypt."
},
{
"LastName": "Abo-Louz",
"FirstName": "Ashraf",
"Affiliation": "Department of Obstetrics & Gynecology, October 6th University, Giza, Egypt."
}
] | Yes |
39014082 | Identification of a pro-protein synthesis osteosarcoma subtype for predicting prognosis and treatment. | Osteosarcoma (OS) is a heterogeneous malignant spindle cell tumor that is aggressive and has a poor prognosis. Although combining surgery and chemotherapy has significantly improved patient outcomes, the prognosis for OS patients with metastatic or recurrent OS has remained unsatisfactory. Therefore, it is imperative to gain a fresh perspective on OS development mechanisms and treatment strategies. After studying single-cell RNA sequencing (scRNA-seq) data in public databases, we identified seven OS subclonal types based on intra-tumor heterogeneity. Subsequently, we constructed a prognostic model based on pro-protein synthesis osteosarcoma (PPS-OS)-associated genes. Correlation analysis showed that the prognostic model performs extremely well in predicting OS patient prognosis. We also demonstrated that the independent risk factors for the prognosis of OS patients were tumor primary site, metastatic status, and risk score. Based on these factors, nomograms were constructed for predicting the 3- and 5-year survival rates. Afterward, the investigation of the tumor immune microenvironment (TIME) revealed the vital roles of γδ T-cell and B-cell activation. Drug sensitivity analysis and immune checkpoint analysis identified drugs that have potential application value in OS. Finally, the jumping translocation breakpoint (JTB) gene was selected for experimental validation. JTB silencing suppressed the proliferation, migration, and invasion of OS cells. Therefore, our research suggests that PPS-OS-related genes facilitate the malignant progression of OS and may be employed as prognostic indicators and therapeutic targets in OS. | 2024 Jul 16 | Scientific reports | No DOI | [
{
"LastName": "Yi",
"FirstName": "Chengfeng",
"Affiliation": "Department of Orthopaedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China."
},
{
"LastName": "Liu",
"FirstName": "Jun",
"Affiliation": "Department of Orthopaedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China."
},
{
"LastName": "Zhao",
"FirstName": "Shibing",
"Affiliation": "Department of Orthopaedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China."
},
{
"LastName": "Gong",
"FirstName": "Deliang",
"Affiliation": "Department of Orthopaedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China."
},
{
"LastName": "Xu",
"FirstName": "Bohan",
"Affiliation": "Department of Orthopaedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China."
},
{
"LastName": "Li",
"FirstName": "Ao",
"Affiliation": "Department of Orthopaedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China."
},
{
"LastName": "Bian",
"FirstName": "Erbao",
"Affiliation": "Department of Orthopaedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China. bianerbao@ahmu.edu.cn."
},
{
"LastName": "Tian",
"FirstName": "Dasheng",
"Affiliation": "Department of Orthopaedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China. tiands1212@126.com."
}
] | No |
38950371 | Capturing alterations of intracellular-extracellular lactate distribution in the brain using diffusion-weighted MR spectroscopy in vivo. | While the intracellular-extracellular distribution of lactate has been suggested to play a critical role in the healthy and diseased brain, tools are lacking to noninvasively probe lactate in intracellular and extracellular spaces. Here, we show that, by measuring the diffusion of lactate with diffusion-weighted magnetic resonance (MR) spectroscopy in vivo and comparing it to the diffusion of purely intracellular metabolites, noninvasive quantification of extracellular and intracellular lactate fractions becomes possible. More specifically, we detect alterations of lactate diffusion in the APP/PS1 mouse model of Alzheimer's disease. Data modeling allows quantifying decreased extracellular lactate fraction in APP/PS1 mice as compared to controls, which is quantitatively confirmed with implanted enzyme-microelectrodes. The capability of diffusion-weighted MR spectroscopy to quantify extracellular-intracellular lactate fractions opens a window into brain metabolism, including in Alzheimer's disease. | 2024 Jul 9 | Proceedings of the National Academy of Sciences of the United States of America | No DOI | [
{
"LastName": "Malaquin",
"FirstName": "Sophie",
"Affiliation": "Laboratoire des Maladies Neurodégénératives, Université Paris-Saclay, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), CNRS, Molecular Imaging Research Center (MIRCen), Fontenay-aux-Roses 92260, France."
},
{
"LastName": "Lerchundi",
"FirstName": "Rodrigo",
"Affiliation": "Laboratoire des Maladies Neurodégénératives, Université Paris-Saclay, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), CNRS, Molecular Imaging Research Center (MIRCen), Fontenay-aux-Roses 92260, France."
},
{
"LastName": "Mougel",
"FirstName": "Eloïse",
"Affiliation": "Laboratoire des Maladies Neurodégénératives, Université Paris-Saclay, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), CNRS, Molecular Imaging Research Center (MIRCen), Fontenay-aux-Roses 92260, France."
},
{
"LastName": "Valette",
"FirstName": "Julien",
"Affiliation": "Laboratoire des Maladies Neurodégénératives, Université Paris-Saclay, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), CNRS, Molecular Imaging Research Center (MIRCen), Fontenay-aux-Roses 92260, France."
}
] | No |
38863817 | Realization of a green-emitting pyrosilicate-structured Er(3+)-activated Y(2)Si(2)O(7) phosphor: a systematic study of opto-electronic characteristics and thermal stability for lighting applications. | A series of green-emitting Y2-x Si2O7:xEr[3+] phosphors (x = 1-7 mol%) have been successfully synthesized using a straightforward gel-combustion method facilitated by urea. X-ray diffraction analysis provided specific patterns for samples, confirming a consistent triclinic phase across erbium-doped structures compared to undoped structures. Studies using TEM and EDX were conducted to identify the surface-related characteristics and chemical composition of the synthesized nanophosphor, respectively. The band gap was determined to be 5.55 eV and 5.80 eV for the host material and optimal sample, respectively. The primary peak of excitation, observed at 379 nm, represents the highly sensitive electric dipole transition from the [4]I15/2 state to the [4]G11/2 level, suggesting that the prepared phosphors could effectively absorb NUV light for activation. The PL profiles of Y2-x Si2O7:xEr[3+] (x = 1-7 mol%) phosphors demonstrate characteristic emissions at 409 nm ([2]H9/2 → [4]I15/2), 522 nm ([2]H11/2 → [4]I15/2), 553 nm ([4]S3/2 → [4]I15/2) and 662 nm ([4]F9/2 → [4]I15/2). In accordance with Dexter's theory, luminescence quenching observed at a concentration of 4 mol% Er[3+] is attributed to dipole-quadrupole interactions. The optimal sample demonstrates excellent thermal stability, indicated by its luminescence at different temperatures and activation energy of 0.2641 eV. Additionally, the CIE, color purity and CCT values of the fabricated nanomaterials make it ideal for use in lighting applications. | 2024 Jun 6 | RSC advances | No DOI | [
{
"LastName": "Kumar",
"FirstName": "Pawan",
"Affiliation": "Department of Chemistry, Maharshi Dayanand University Rohtak-124001 Haryana India devjakhar@gmail.com."
},
{
"LastName": "Singh",
"FirstName": "Devender",
"Affiliation": "Department of Chemistry, Maharshi Dayanand University Rohtak-124001 Haryana India devjakhar@gmail.com."
},
{
"LastName": "Singh",
"FirstName": "Sitender",
"Affiliation": "Department of Chemistry, Maharshi Dayanand University Rohtak-124001 Haryana India devjakhar@gmail.com."
},
{
"LastName": "Kumar",
"FirstName": "Harish",
"Affiliation": "Department of Chemistry, School of Chemical Sciences, Central University of Haryana Mahendergarh-123031 India."
},
{
"LastName": "Kumar",
"FirstName": "Ramesh",
"Affiliation": "Department of Chemistry, Kurukshetra University Kurukshetra-136119 Haryana India."
}
] | No |
35747487 | Lockdown cardiomyopathy: from a COVID-19 pandemic to a loneliness pandemic. | Social distancing/isolation is vital for infection control but can adversely impact on mental health. As the spread of COVID-19 is contained, mental health issues will surface with particular concerns for elderly, isolated populations. We present a case of Takotsubo cardiomyopathy related to lockdown anxiety. | 2021 | The British journal of cardiology | No DOI | [
{
"LastName": "Sekar",
"FirstName": "Baskar",
"Affiliation": "Cardiology Interventional Fellow Morriston Cardiac Centre, Morriston Hospital, Swansea, SA6 SNL."
},
{
"LastName": "Kurdi",
"FirstName": "Hibba",
"Affiliation": "Cardiology Fellow Morriston Cardiac Centre, Morriston Hospital, Swansea, SA6 SNL."
},
{
"LastName": "Smith",
"FirstName": "David",
"Affiliation": "Consultant Cardiologist Morriston Cardiac Centre, Morriston Hospital, Swansea, SA6 SNL."
}
] | No |
22221691 | Interference of patent blue dye with pulse oximetry readings, methemoglobin measurements, and blue urine in sentinel lymph node mapping: a case report and review of the literature. | Patent blue (PB) dye has been successfully used worldwide in breast and cervix surgeries with few complications. Interference of oxyhemoglobin saturation reading by pulse oximetry (SpO(2)) and methemoglobinemia, from injection of PB dye, have rarely been reported in breast and cervix surgeries. We report here the first case of interference of SpO(2) reading, advent of methemoglobinemia, and blue urine from the use of PB dye, which occurred concurrently in a female undergoing bilateral modified radical mastectomy. The unexpected events might be a consequence of excessive administration of PB dye. We also reviewed the published discourses in literature on the adverse effects of PB dye. | 2011 Dec | Acta anaesthesiologica Taiwanica : official journal of the Taiwan Society of Anesthesiologists | No DOI | [
{
"LastName": "Lai",
"FirstName": "Hou-Chuan",
"Affiliation": "Department of Anesthesiology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan."
},
{
"LastName": "Hsu",
"FirstName": "Huan-Ming",
"Affiliation": ""
},
{
"LastName": "Cherng",
"FirstName": "Chen-Hwan",
"Affiliation": ""
},
{
"LastName": "Lin",
"FirstName": "Shinn-Long",
"Affiliation": ""
},
{
"LastName": "Wu",
"FirstName": "Ching-Tang",
"Affiliation": ""
},
{
"LastName": "Yu",
"FirstName": "Jyh-Cherng",
"Affiliation": ""
},
{
"LastName": "Yeh",
"FirstName": "Chun-Chang",
"Affiliation": ""
}
] | No |
37231965 | CSF Aβ40 Levels Do Not Correlate with the Clinical Manifestations of Alzheimer's Disease. | Cerebrospinal fluid (CSF) biomarker quantification provides physicians with a reliable diagnosis of Alzheimer's disease (AD). However, the relationship between their concentration and disease course has not been clearly elucidated. This work aimed to investigate the clinical and prognostic significance of Aβ40 CSF levels. A retrospective cohort of 76 patients diagnosed with AD using a decreased Aβ42/Aβ40 ratio was subclassified into hyposecretors (Aβ40 <7,755 pg/mL), normosecretors (Aβ40 7,755-16,715 pg/mL), and hypersecretors (Aβ40 >16,715 pg/mL). Potential differences in AD phenotype, Montreal Cognitive Assessment (MoCA) scores, and Global Deterioration Scale (GDS) stages were assessed. Correlation tests for biomarker concentrations were also performed. Participants were classified as hyposecretors (n = 22, median Aβ40 5,870.500 pg/mL, interquartile range [IQR] 1,431), normosecretors (n = 47, median Aβ40 10,817 pg/mL, IQR 3,622), and hypersecretors (n = 7, 19,767 pg/mL, IQR 3,088). The distribution of positive phosphorylated Tau (p-Tau) varied significantly between subgroups and was more common in the normo- and hypersecretor categories (p = 0.003). Aβ40 and p-Tau concentrations correlated positively (ρ = 0.605, p < 0.001). No significant differences were found among subgroups regarding age, initial MoCA score, initial GDS stage, progression to the dementia stage, or changes in the MoCA score. In this study, we found no significant differences in clinical symptoms or disease progression in AD patients according to their CSF Aβ40 concentration. Aβ40 was positively correlated with p-Tau and total Tau concentrations, supporting their potential interaction in AD pathophysiology. | 2022 | Neuro-degenerative diseases | No DOI | [
{
"LastName": "Garcia Castro",
"FirstName": "Jesús",
"Affiliation": "Department of Neurology, Hospital Universitario La Paz, Hospital La Paz Institute for Health Research - IdiPAZ, Madrid, Spain, jesusgcastro14@gmail.com."
},
{
"LastName": "Méndez Del Sol",
"FirstName": "Helena",
"Affiliation": "Department of Clinical Analysis, Hospital Universitario La Paz, Madrid, Spain."
},
{
"LastName": "Rodríguez Fraga",
"FirstName": "Olaia",
"Affiliation": "Department of Clinical Analysis, Hospital Universitario La Paz, Madrid, Spain."
},
{
"LastName": "Hernández Barral",
"FirstName": "María",
"Affiliation": "Department of Neurology, Hospital Universitario La Paz, Hospital La Paz Institute for Health Research - IdiPAZ, Madrid, Spain."
},
{
"LastName": "Serrano López",
"FirstName": "Soledad",
"Affiliation": "Department of Neurology, Hospital Universitario La Paz, Hospital La Paz Institute for Health Research - IdiPAZ, Madrid, Spain."
},
{
"LastName": "Frank García",
"FirstName": "Ana",
"Affiliation": "Department of Neurology, Hospital Universitario La Paz, Hospital La Paz Institute for Health Research - IdiPAZ, Madrid, Spain."
},
{
"LastName": "Martín Montes",
"FirstName": "Ángel",
"Affiliation": "Department of Neurology, Hospital Universitario La Paz, Hospital La Paz Institute for Health Research - IdiPAZ, Madrid, Spain."
}
] | No |
37661201 | Comparative metabolomic and transcriptomic analysis of Saccharomyces cerevisiae W303a and CEN.PK2-1C. | Saccharomyces cerevisiae is a health microorganism closely related to human life, especially in food and pharmaceutical industries. S. cerevisiae W303a and CEN.PK2-1C are two commonly used strains for synthetic biology-based natural product production. Yet, the metabolomic and transcriptomic differences between these two strains have not been compared. In this study, metabolomics and transcriptomics were applied to analyze the differential metabolites and differential expression genes (DEGs) between W303a and CEN.PK2-1C cultured in YPD and SD media. The growth rate of W303a in YPD medium was the lowest compared with other groups. When cultured in YPD medium, CEN.PK2-1C produced more phenylalanine than W303a; when cultured in SD medium, W303a produced more phospholipids than CEN.PK2-1C. Transcriptomic analysis revealed that 19 out of 22 genes in glycolysis pathway were expressed at higher levels in CEN.PK2-1C than that in W303a no matter which media were used, and three key genes related to phenylalanine biosynthesis including ARO9, ARO7 and PHA2 were up-regulated in CEN.PK2-1C compared with W303a when cultured in YPD medium, whereas seven DEGs associated with phospholipid biosynthesis were up-regulated in W303a compared with CEN.PK2-1C when cultured in SD medium. The high phenylalanine produced by CEN.PK2-1C and high phospholipids produced by W303a indicated that CEN.PK2-1C may be more suitable for synthesis of natural products with phenylalanine as precursor, whereas W303a may be more appropriate for synthesis of phospholipid metabolites. This finding provides primary information for strain selection between W303a and CEN.PK2-1C for synthetic biology-based natural product production. | 2023 Sep 4 | World journal of microbiology & biotechnology | No DOI | [
{
"LastName": "Zhang",
"FirstName": "Meihong",
"Affiliation": "The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China."
},
{
"LastName": "Zhang",
"FirstName": "Jinjia",
"Affiliation": "The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China."
},
{
"LastName": "Hou",
"FirstName": "Maoqi",
"Affiliation": "The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China."
},
{
"LastName": "Zhao",
"FirstName": "Shujuan",
"Affiliation": "The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. zhaoshujuan@126.com."
}
] | No |
35655371 | Comparison of the soleus and plantaris muscles in humans and other primates: Macroscopic neuromuscular anatomy and evolutionary significance. | In humans, the soleus is more developed compared to other primates and has a unique architecture composed of anterior bipennate and posterior unipennate parts, which are innervated by different nerve branches. The anterior part of the human soleus was proposed to be important for bipedalism, however, the phylogenetic process resulting in its acquisition remains unclear. Providing insights into this process, the anterior part of the soleus was suggested to be closely related to the plantaris based on the branching pattern of their nerve fascicles. To reveal the phylogeny of the soleus and plantaris in primates, the innervation patterns of the posterior crural muscles were compared among a wide range of species. From their branching pattern, posterior crural muscles could be classified into superficial and deep muscle groups. The anterior part of the soleus and plantaris both belonged to the deep muscle group. In all the examined specimens of ring-tailed lemurs and chimpanzees, as well as in one out of two specimens of siamang, the nerve branches corresponding to those innervating the anterior part of the human soleus were found. The muscular branches innervating the anterior part of the soleus and plantaris formed a common trunk or were connected in all the specimens. These results indicate that the anterior part of the soleus is closely related to the plantaris across different species of primates. In turn, this suggests that the anterior part of the soleus is maintained among primates, and especially in humans, where it develops as the characteristic bipennate structure. | 2023 Feb | Anatomical record (Hoboken, N.J. : 2007) | No DOI | [
{
"LastName": "Sakuraya",
"FirstName": "Tohma",
"Affiliation": "Department of Rehabilitation Sciences, Kobe University Graduate School of Health Sciences, Kobe, Japan."
},
{
"LastName": "Sekiya",
"FirstName": "Shin-Ichi",
"Affiliation": "Faculty of Nursing, Niigata College of Nursing, Joetsu, Japan."
},
{
"LastName": "Emura",
"FirstName": "Kenji",
"Affiliation": "Faculty of Health Care Sciences, Himeji Dokkyo University, Himeji, Japan."
},
{
"LastName": "Sonomura",
"FirstName": "Takahiro",
"Affiliation": "Department of Anatomy, Division of Oral Structure, Function and Development, Asahi University School of Dentistry, Mizuho, Gifu, Japan."
},
{
"LastName": "Hirasaki",
"FirstName": "Eishi",
"Affiliation": "Section of Evolutionary Morphology, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan."
},
{
"LastName": "Arakawa",
"FirstName": "Takamitsu",
"Affiliation": "Department of Rehabilitation Sciences, Kobe University Graduate School of Health Sciences, Kobe, Japan."
}
] | No |
38031195 | Chronic myeloid leukemia with sleep-related painful erections as a first symptom: a case report. | Sleep-related painful erections are characterized by deep penile pain that occurs during erections in the rapid eye movement stage of sleep. This case presents a 43-year-old Chinese Han patient with sleep-related painful erections. Turgid painful erections (4-5 episodes of tumescence) during the sleep hours caused pain. Further, blood testing revealed an abnormal increase in white blood cells (123 × 10[9]/L). The patient was diagnosed with chronic myeloid leukemia by bone marrow biopsy, BCR::ABL1 fusion gene testing, and Philadelphia chromosome. However, the sleep-related painful erections have dramatically decreased in frequency of erectile pain after chemotherapy for Chronic myeloid leukemia in our case. We considered that the occurrence of sleep-related painful erections was related to chronic myeloid leukemia and the case might be secondary sleep-related painful erections. | 2023 Nov 30 | Journal of medical case reports | No DOI | [
{
"LastName": "Han",
"FirstName": "Yao-Dong",
"Affiliation": "Department of Hematology, The First People's Hospital of Lanzhou, Lanzhou, 730050, China."
},
{
"LastName": "Chen",
"FirstName": "Hong-Jie",
"Affiliation": "Department of Urology, The First People's Hospital of Lanzhou, Lanzhou, 730050, China. cyr2000816@sina.com."
}
] | No |
37274462 | MicroRNAs associated with postoperative outcomes in patients with limited stage neuroendocrine carcinoma of the esophagus. | Esophageal neuroendocrine carcinoma (E-NEC) is an aggressive disease with a poor prognosis. The present study aimed to assess the role of surgery in the treatment of patients with resectable E-NEC, and identify a microRNA (miRNA/miR) signature in association with positive postoperative outcomes. Between February 2017 and August 2019, 36 patients with E-NEC who underwent curative surgery at the Japan Neuroendocrine Tumor Society partner hospitals were enrolled in the study. A total of 16 (44.4%) patients achieved disease-free survival (non-relapse group), whereas 20 (55.6%) patients developed tumor relapse (relapse group) during the median follow-up time of 36.5 months (range, 1-242) after surgery with a 5-year overall survival rate of 100 and 10.8%, respectively (P<0.01). No clinicopathological parameters, such as histological type or TNM staging, were associated with tumor relapse. Microarray analysis of 2,630 miRNAs in 11 patients with sufficient quality RNA revealed 12 miRNAs (miR-1260a, -1260b, -1246, -4284, -612, -1249-3p, -296-5p, -575, -6805-3p, -12136, -6822-5p and -4454) that were differentially expressed between the relapse (n=6) and non-relapse (n=5) groups. Furthermore, the top three miRNAs (miR-1246, -1260a and -1260b) were associated with overall survival (P<0.01). These results demonstrated that surgery-based multidisciplinary treatment is effective in a distinct subpopulation of limited stage E-NEC. A specific miRNA gene set is suggested to be associated with treatment outcome. | 2023 Jul | Oncology letters | No DOI | [
{
"LastName": "Okumura",
"FirstName": "Tomoyuki",
"Affiliation": "Department of Surgery and Science, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama 930-0194, Japan."
},
{
"LastName": "Fujii",
"FirstName": "Tsutomu",
"Affiliation": "Department of Surgery and Science, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama 930-0194, Japan."
},
{
"LastName": "Terabayashi",
"FirstName": "Kenji",
"Affiliation": "Department of Mechanical and Intellectual Systems Engineering, Faculty of Engineering, University of Toyama, Toyama 930-8555, Japan."
},
{
"LastName": "Kojima",
"FirstName": "Takashi",
"Affiliation": "Department of Gastroenterology and Gastrointestinal Oncology, National Cancer Center Hospital East, Kashiwa, Chiba 277-8577, Japan."
},
{
"LastName": "Takeda",
"FirstName": "Shigeru",
"Affiliation": "Department of Gastroenterological, Breast and Endocrine Surgery, Graduate School of Medicine, Yamaguchi University, Ube, Yamaguchi 755-8505, Japan."
},
{
"LastName": "Kashiwada",
"FirstName": "Tomomi",
"Affiliation": "Department of Medical Oncology, Division Hematology, Respiratory Medical and Oncology, Saga University, Saga 849-8501, Japan."
},
{
"LastName": "Toriyama",
"FirstName": "Kazuhiro",
"Affiliation": "Department of Gastroenterology, Aichi Cancer Center Hospital, Nagoya, Aichi 464-8681, Japan."
},
{
"LastName": "Hijioka",
"FirstName": "Susumu",
"Affiliation": "Department of Gastroenterology, Aichi Cancer Center Hospital, Nagoya, Aichi 464-8681, Japan."
},
{
"LastName": "Miyazaki",
"FirstName": "Tatsuya",
"Affiliation": "Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan."
},
{
"LastName": "Yamamoto",
"FirstName": "Miho",
"Affiliation": "Department of Gastroenterological Surgery, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan."
},
{
"LastName": "Tanabe",
"FirstName": "Shunsuke",
"Affiliation": "Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan."
},
{
"LastName": "Shirakawa",
"FirstName": "Yasuhiro",
"Affiliation": "Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan."
},
{
"LastName": "Furukawa",
"FirstName": "Masayuki",
"Affiliation": "Department of Hepato-Biliary-Pancreatology, National Hospital Organization Kyushu Cancer Center, Fukuoka 811-1395, Japan."
},
{
"LastName": "Honma",
"FirstName": "Yoshitaka",
"Affiliation": "Department of Head and Neck, Esophageal Medical Oncology, National Cancer Center Hospital, Tokyo 104-0045, Japan."
},
{
"LastName": "Hoshino",
"FirstName": "Isamu",
"Affiliation": "Division of Gastroenterological Surgery, Chiba Cancer Center, Chiba 260-8717, Japan."
},
{
"LastName": "Nabeya",
"FirstName": "Yoshihiro",
"Affiliation": "Division of Gastroenterological Surgery, Chiba Cancer Center, Chiba 260-8717, Japan."
},
{
"LastName": "Yamaguchi",
"FirstName": "Hironori",
"Affiliation": "Department of Clinical Oncology, Jichi Medical University, Shimotsuke, Tochigi 329-0498, Japan."
},
{
"LastName": "Uemoto",
"FirstName": "Shinji",
"Affiliation": "President's Office, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan."
},
{
"LastName": "Shimada",
"FirstName": "Yutaka",
"Affiliation": "Department of Nanobio Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan."
},
{
"LastName": "Matsubara",
"FirstName": "Hisahiro",
"Affiliation": "Department of Frontier Surgery, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan."
},
{
"LastName": "Ozawa",
"FirstName": "Soji",
"Affiliation": "Department of Gastroenterological Surgery, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan."
},
{
"LastName": "Makuuchi",
"FirstName": "Hiroyasu",
"Affiliation": "Department of Gastroenterological Surgery, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan."
},
{
"LastName": "Imamura",
"FirstName": "Masayuki",
"Affiliation": "Neuroendocrine Tumor Center, Kansai Electric Power Hospital, Osaka 553-0003, Japan."
}
] | No |
23343946 | Interaction of nutrition and infections globally: an overview. | The interaction of nutrition and infections is known by experience by generations of medical doctors. Before the era of antibiotics, diet was an integral part of the management of infections. Now, it is necessary to take a fresh look at this interaction as the understanding of immune response has expanded considerably. Comparatively little research has addressed the impact of nutrition interventions on the management of infectious diseases. Most observations of the interaction between nutrition and infections are epidemiological in character. This holds especially true for measles as well as for tuberculosis. In AIDS, the deterioration of the nutritional status is an indicator of disease progression. Infections in undernourished children are a common cause of death, and taking this finding into account helps to reduce the case fatality rate in severely malnourished patients. Regarding the immune response, cellular as well as soluble components are affected by deficiencies of single nutrients or general undernutrition. The immunosuppressive effect of undernutrition starts during intrauterine life already: maternal nutrition status has been shown to impact on immune function in adult animals. Recent research suggests that not only undernutrition but also caloric overnutrition impacts on immune response to infections and immunization. This is partly due to the chronic inflammatory activity of the adipose tissue and partly due to neuroendocrine alterations. Infectious diseases also impact on the nutritional status, either specifically or through unspecific mechanisms, such as anorexia, tachypnea, and vomiting. | 2012 | Annals of nutrition & metabolism | No DOI | [
{
"LastName": "Krawinkel",
"FirstName": "M B",
"Affiliation": "Institute of Nutritional Sciences, Justus-Liebig University, Giessen, Germany."
}
] | No |
26862298 | Carbamylated Erythropoietin: A Prospective Drug Candidate for Neuroprotection. | Carbamylated erythropoietin (cEpo), which is neuroprotective but lacks hematopoietic activity, has been attracting rising concerns. However, the cellular and molecular mechanisms involved in the process of neuroprotection of cEpo are not well known. Based on several recent reports, the neuroprotective effects of cEpo are illustrated, and signaling pathways involved in the different effects of erythropoietin and cEpo are discussed. These newly reported researches may shed new light on the development and application of cEpo, a prospective drug candidate for neuroprotection. | 2015 | Biochemistry insights | No DOI | [
{
"LastName": "Chen",
"FirstName": "Jianmin",
"Affiliation": "School of Basic Medical Sciences, Chengdu Medical College, Chengdu, China."
},
{
"LastName": "Yang",
"FirstName": "Zheng",
"Affiliation": "School of Basic Medical Sciences, Chengdu Medical College, Chengdu, China."
},
{
"LastName": "Zhang",
"FirstName": "Xiao",
"Affiliation": "School of Basic Medical Sciences, Chengdu Medical College, Chengdu, China."
}
] | No |
1967819 | Thymopoietin, a thymic polypeptide, regulates nicotinic alpha-bungarotoxin sites in chromaffin cells in culture. | The identity of the neuronal nicotinic alpha-bungarotoxin (alpha-BGT) site, which appears to be distinct from the functional nicotinic receptor, is unclear. Recent work in our laboratory has shown that the thymus-derived polypeptide thymopoietin potently and specifically interacts at the nicotinic alpha-BGT site in brain. The present results show that thymopoietin also interferes with the binding of 125I-alpha-BGT to chromaffin cells in culture; a dose-dependent inhibition in binding was observed, with an IC50 of 10(-8) M. To assess the long term effect(s) of thymopoietin in nervous tissue, chromaffin cells were exposed to the polypeptide for varying periods of time. Incubation of the cells in culture with thymopoietin (10(-9) to 3 x 10(-7) M) for 2 to 7 days resulted in an approximate 3-fold increase in alpha-BGT binding. Saturation analysis indicated this was due to an increase in the Bmax. The thymopoietin-induced increase in binding could be reversed with nicotine: thus, the sites can be regulated by a nicotinic receptor ligand. Although thymopoietin potently interacted at the nicotinic alpha-BGT receptor, nicotinic receptor responsiveness was not affected after short or long term exposure to the peptide. Neither basal nor nicotinic receptor-stimulated tyrosine hydroxylase activity was altered by thymopoietin. As well, resting and acetylcholine-evoked noradrenaline release remained similar to control after exposure of the cells to the polypeptide. These results indicate that the thymic polypeptide thymopoietin specifically interacts with the nicotinic alpha-BGT receptor population and, furthermore, can regulate the toxin binding sites in chromaffin cells in culture. | 1990 Jan | Molecular pharmacology | No DOI | [
{
"LastName": "Quik",
"FirstName": "M",
"Affiliation": "Department of Pharmacology, McGill University, Montreal, Quebec, Canada."
},
{
"LastName": "Afar",
"FirstName": "R",
"Affiliation": ""
},
{
"LastName": "Geertsen",
"FirstName": "S",
"Affiliation": ""
},
{
"LastName": "Audhya",
"FirstName": "T",
"Affiliation": ""
},
{
"LastName": "Goldstein",
"FirstName": "G",
"Affiliation": ""
},
{
"LastName": "Trifaró",
"FirstName": "J M",
"Affiliation": ""
}
] | Yes |
26095777 | Development, metamorphosis, morphology, and diversity: The evolution of chordate muscles and the origin of vertebrates. | Recent findings that urochordates are the closest sister-group of vertebrates have dramatically changed our understanding of chordate evolution and vertebrate origins. To continue to deepen our understanding of chordate evolution and diversity, in particular the morphological and taxonomical diversity of the vertebrate clade, one must explore the origin, development, and comparative anatomy of not only hard tissues, but also soft tissues such as muscles. Building on a recent overview of the discovery of a cardiopharyngeal field in urochordates and the profound implications for reconstructing the origin and early evolution of vertebrates, in this study we focus on the broader comparative and developmental anatomy of chordate cephalic muscles and their relation to life history, and to developmental, morphological and taxonomical diversity. We combine our recent findings on cephalochordates, urochordates, and vertebrates with a literature review and suggest that developmental changes related to metamorphosis and/or heterochrony (e.g., peramorphosis) played a crucial role in the early evolution of chordates and vertebrates. Recent studies reviewed here supported de Beer's "law of diversity" that peramorphic animals (e.g., ascidians, lampreys) are taxonomically and morphologically less diverse than nonperamorphic animals (e.g., gnathostomes), probably because their "too specialized" development and adult anatomy constrain further developmental and evolutionary innovations. Developmental Dynamics 244:1046-1057, 2015. © 2014 Wiley Periodicals, Inc. | 2015 Sep | Developmental dynamics : an official publication of the American Association of Anatomists | No DOI | [
{
"LastName": "Diogo",
"FirstName": "Rui",
"Affiliation": "Department of Anatomy, Howard University College of Medicine, Washington, DC."
},
{
"LastName": "Ziermann",
"FirstName": "Janine M",
"Affiliation": "Department of Anatomy, Howard University College of Medicine, Washington, DC."
}
] | Yes |
29412697 | miR-155 Affects Osteosarcoma MG-63 Cell Autophagy Induced by Adriamycin Through Regulating PTEN-PI3K/AKT/mTOR Signaling Pathway. | Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) plays a positive regulatory role on cell autophagy through inhibiting PI3K/AKT/mammalian target of rapamycin (mTOR) signaling pathway. miR-155 plays a critical role in osteosarcoma occurrence and chemoresistance. Bioinformatics analysis revealed the targeted binding site between miR-155 and the 3'-UTR (untranslated region) of PTEN mRNA. This study investigated the role of miR-155 in regulating osteosarcoma cell autophagy, chemosensitivity to Adriamycin (ADM), and PTEN-PI3K/AKT/mTOR signaling pathway. Dual luciferase reporter gene assay confirmed the relationship between miR-155 and PTEN. MG-63 cells and drug-resistant MG-63/ADM cells were treated by ADM to compare miR-155, PTEN, p-AKT, p-mTOR, and Beclin-1 expressions. Cell apoptosis was tested by flow cytometry. MG-63/ADM cells were divided into five groups, including anti-miR-NC, anti-miR-155, pSicoR-blank, pSicoR-PTEN, and anti-miR-155+pSicoR-PTEN group. miR-155 targeted suppressed PTEN expression. miR-155, p-AKT, and p-mTOR significantly increased, while PTEN and Beclin-1 obviously reduced in MG-63/ADM cells compared with MG-63 cells. ADM treatment markedly elevated miR-155, p-AKT, and p-mTOR expressions, whereas reduced PTEN level. Beclin-1 was slightly upregulated, and autophagy and apoptosis levels were low. Anti-miR-155 and/or pSicoR-PTEN significantly enhanced PTEN and Beclin-1 expressions, cell apoptosis, and autophagy induced by ADM and declined p-AKT and p-mTOR levels. miR-155 targeted suppressed PTEN expression, enhanced PI3K/AKT/mTOR signaling pathway, inhibited cell apoptosis and autophagy induced by ADM, and reduced sensitivity to ADM. | 2018 Feb | Cancer biotherapy & radiopharmaceuticals | No DOI | [
{
"LastName": "Wang",
"FirstName": "Lin",
"Affiliation": "Department of Orthopedics Institute, Affiliated Dongfeng Hospital, Hubei University of Medicine , Shiyan, China ."
},
{
"LastName": "Tang",
"FirstName": "Bing",
"Affiliation": "Department of Orthopedics Institute, Affiliated Dongfeng Hospital, Hubei University of Medicine , Shiyan, China ."
},
{
"LastName": "Han",
"FirstName": "Heng",
"Affiliation": "Department of Orthopedics Institute, Affiliated Dongfeng Hospital, Hubei University of Medicine , Shiyan, China ."
},
{
"LastName": "Mao",
"FirstName": "Dan",
"Affiliation": "Department of Orthopedics Institute, Affiliated Dongfeng Hospital, Hubei University of Medicine , Shiyan, China ."
},
{
"LastName": "Chen",
"FirstName": "Jie",
"Affiliation": "Department of Orthopedics Institute, Affiliated Dongfeng Hospital, Hubei University of Medicine , Shiyan, China ."
},
{
"LastName": "Zeng",
"FirstName": "Yun",
"Affiliation": "Department of Orthopedics Institute, Affiliated Dongfeng Hospital, Hubei University of Medicine , Shiyan, China ."
},
{
"LastName": "Xiong",
"FirstName": "Min",
"Affiliation": "Department of Orthopedics Institute, Affiliated Dongfeng Hospital, Hubei University of Medicine , Shiyan, China ."
}
] | Yes |
20169920 | Abnormal morphology of skeletal muscles in meat-type chickens--ultrastructural observations. | In order to determine the effect of different production systems on muscle ultrastructure in meat-type chickens, we examined m. gastrocnemius and m. pectoralis superficialis in two lines of chickens (Anak Titan and Isa 215) raised in three different technological systems (indoors in a conventional facility, indoors with limited outdoor access and outdoors with an umbrella roof). Our previous study showed some abnormalities in the histological structure of these muscles. We hypothesized that electron microscopy, having a strategic position in muscle examination, would provide insight into changes in muscle tissue revealed by light microscopy. The results of the study indicate that the muscles examined undergo ultrastructural alterations regardless of the muscle type, chicken line and production system. The abnormalities observed in the present study were found to affect many aspects of fiber ultrastructure impairing function of the myofibrillar apparatus--the structure of mitochondria and sarcoplasmic reticulum, defects of the sarcolemma as well as the appearance of remnants resulting from fiber disintegration. Abnormal responses were found primarily in myofibrils and mitochondria, and--to a lesser extent--in other structures of muscle fibers. We suggest that the majority of the changes observed may lead to muscle damage followed by pathology. The severity of these changes was particularly evident in the muscles of chickens of the Isa 215 line (highly selected) kept outdoors with an umbrella roof. The observations point to a dependence of the above changes on the line of chickens and rearing conditions. Therefore, the limited potential of highly selected broilers to adapt to different environmental conditions should be taken into account while selecting a new production technology. | 2009 | Polish journal of veterinary sciences | No DOI | [
{
"LastName": "Polak",
"FirstName": "M",
"Affiliation": "Department of Poultry Science, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Poland."
},
{
"LastName": "Przybylska-Gornowicz",
"FirstName": "B",
"Affiliation": ""
},
{
"LastName": "Faruga",
"FirstName": "A",
"Affiliation": ""
}
] | No |
20976297 | Synthesis, X-Ray Structure, and Characterization of Catena-bis(benzoate)bis{N,N-bis(2-hydroxyethyl)glycinate}cadmium(II). | The reaction of N, N-bis(2-hydroxyethyl)glycine (bicine; bicH(3)) with Cd(O(2)CPh)(2) · 2H(2)O in MeOH yielded the polymeric compound [Cd(2)(O(2)CPh)(2)(bicH(2))(2)](n)(1). The complex crystallizes in the tetragonal space group P4(1)2(1)2. The lattice constants are a = b = 12.737(5) and c = 18.288(7) Å. The compound contains chains of repeating {Cd(2)(O(2)CPh)(2)(bicH(2))(2)} units. One Cd(II) atom is coordinated by two carboxylate oxygen, four hydroxyl oxygen, and two nitrogen atoms from two symmetry-related 2.21111 (Harris notation) bicH(2) (-) ligands. The other Cd(II) atom is coordinated by six carboxylate oxygen atoms, four from two bicH(2) (-) ligands and two from the monodentate benzoate groups. Each bicinate(-1) ligand chelates the 8-coordinate, square antiprismatic Cd(II) atom through one carboxylate oxygen, the nitrogen, and both hydroxyl oxygen atoms and bridges the second, six-coordinate trigonal prismatic Cd(II) center through its carboxylate oxygen atoms. Compound 1 is the first structurally characterized cadmium(II) complex containing any anionic form of bicine as ligand. IR data of 1 are discussed in terms of the coordination modes of the ligands and the known structure. | 2010 | Bioinorganic chemistry and applications | No DOI | [
{
"LastName": "Katsoulakou",
"FirstName": "Eugenia",
"Affiliation": "Department of Chemistry, University of Patras, 265 04 Patras, Greece."
},
{
"LastName": "Konidaris",
"FirstName": "Konstantis F",
"Affiliation": ""
},
{
"LastName": "Raptopoulou",
"FirstName": "Catherine P",
"Affiliation": ""
},
{
"LastName": "Psyharis",
"FirstName": "Vassilis",
"Affiliation": ""
},
{
"LastName": "Manessi-Zoupa",
"FirstName": "Evy",
"Affiliation": ""
},
{
"LastName": "Perlepes",
"FirstName": "Spyros P",
"Affiliation": ""
}
] | No |
32495864 | Circ_0061140 promotes metastasis of bladder cancer through adsorbing microRNA-1236. | The purpose of this study was to investigate the expression characteristics of circular RNA circ_0061140 in bladder cancer (BCa), and to further explore its effects on invasiveness and migration capacity of BCa cells, as well as its possible potential mechanism. Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to examine the expression level of circ_0061140 in tumor tissue samples and paracancerous ones collected from 42 patients with BCa, and the interplay between circ_0061140 level and the clinical indicators, as well as the prognosis of BCa patients were analyzed. Meanwhile, qRT-PCR was also used to verify circ_0061140 expression in BCa cell lines. In addition, a circ_0061140 knockdown model was constructed using Lentiviral in BCa cell lines, including T24 and 253j, and the effect of circ_0061140 on BCa cell functions and its underlying mechanisms were explored using Cell Counting Kit-8 (CCK-8), transwell, and cell wound healing assays. qPCR results showed that the expression level of circ_0061140 in tumor tissues of BCa patients was remarkably higher than that in adjacent tissues, and the difference was statistically significant. Compared with patients with low expression of circ_0061140, patients with high expression of circ_0061140 had worse prognosis and higher incidence of lymph node or distant metastasis. Compared with those in the negative control group, the proliferation and invasion, as well as the metastasis ability of BCa cells in the sh-circ_0061140 group, were remarkably attenuated. In addition, bioinformatics and Luciferase reporter gene assay demonstrated that circ_0061140 can specifically bind to microRNA-1236. At the same time, the results of qPCR revealed that the expression levels of circ_0061140 and microRNA-1236 were negatively correlated in the tumor tissues of BCa patients. Finally, cell recovery experiment indicated that silencing microRNA-1236 reversed the impact of the knockdown of circ_0061140 on the ability of BCa cells to proliferate and invade, suggesting that the two may regulate each other. Circ_0061140 level was found remarkably elevated in BCa tissues, as well as in cell lines, which was closely relevant to the incidence of lymph node or distant metastasis of BCa patients. In addition, circ_0061140 may enhance the proliferation rate and invasion ability of BCa cells through the modulation of microRNA-1236. | 2020 May | European review for medical and pharmacological sciences | No DOI | [
{
"LastName": "Feng",
"FirstName": "F",
"Affiliation": "Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. guanlin.wu@charite.de."
},
{
"LastName": "Chen",
"FirstName": "A-P",
"Affiliation": ""
},
{
"LastName": "Wang",
"FirstName": "X-L",
"Affiliation": ""
},
{
"LastName": "Wu",
"FirstName": "G-L",
"Affiliation": ""
}
] | Yes |
37634076 | The Impact of Visceral Adiposity on Testosterone Levels in American Adult Men: A Cross-Sectional Analysis. | BACKGROUND Testosterone decline and deficiency importantly affect men's health, and may be associated with excessive deposition of visceral adipose tissue. This study was conducted to explore the association between visceral adiposity index (VAI) and testosterone level. MATERIAL AND METHODS A total of 1551 participants from the NHANES 2013-2013 cycle and 2015-2016 cycle were selected for our analyses. The VAI index was calculated based on waist circumference (WC), body mass index (BMI), triglyceride (TG), and high-density lipoprotein cholesterol (HDL-c), and serum testosterone was measured by isotope dilution liquid chromatography tandem mass spectrometry. Multivariable adjusted linear and logistic regression were utilized to investigate the associations between VAI index and testosterone level and testosterone deficiency, respectively. Additionally, subgroup analyses were performed to identify sensitive populations. RESULTS A total of 1551 participants with mean VAI index of 1.95±0.08 were eligible for our analysis. After adjusting for all potential cofounders, men with higher VAI index displayed a lower level of total testosterone level (ß: -11.74, 95% CI: -17.33, -6.15, P<0.0001), and higher risk of testosterone deficiency (OR: 1.24, 95% CI: 1.09, 1.40, P=0.0022). Comparing to VAI quartile 1, quartile 4 showed the most decreased testosterone level (ß: -94.59, 95% CI: -130.04, -59.14, P<0.0001), and highest risk of testosterone deficiency (OR: 5.07, 95% CI: 2.41,10.63, P<0.0001). Subgroup analysis demonstrated that VAI index was strongly related to testosterone level and testosterone deficiency in aged and obese men. CONCLUSIONS Men with higher VAI index displayed lower testosterone levels and higher risk of testosterone deficiency, especially in aged men and obese men. | 2023 Aug 27 | Medical science monitor : international medical journal of experimental and clinical research | No DOI | [
{
"LastName": "Su",
"FirstName": "Mingqin",
"Affiliation": "Department of Pathology, The Second People's Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical University, Hefei, Anhui, China (mainland)."
},
{
"LastName": "Wei",
"FirstName": "Hongquan",
"Affiliation": "Department of Pathology, The Second People's Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical University, Hefei, Anhui, China (mainland)."
},
{
"LastName": "Chen",
"FirstName": "Lijun",
"Affiliation": "Department of Pathology, The Second People's Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical University, Hefei, Anhui, China (mainland)."
},
{
"LastName": "Guan",
"FirstName": "Yuxiang",
"Affiliation": "Department of Pathology, The Second People's Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical University, Hefei, Anhui, China (mainland)."
},
{
"LastName": "Dong",
"FirstName": "Wei",
"Affiliation": "Department of Pathology, The Second People's Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical University, Hefei, Anhui, China (mainland)."
},
{
"LastName": "Zhao",
"FirstName": "Min",
"Affiliation": "Department of Pathology, The Second People's Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical University, Hefei, Anhui, China (mainland)."
}
] | No |
38489356 | Efficient learning of mixed-state tomography for photonic quantum walk. | Noise-enhanced applications in open quantum walk (QW) has recently seen a surge due to their ability to improve performance. However, verifying the success of open QW is challenging, as mixed-state tomography is a resource-intensive process, and implementing all required measurements is almost impossible due to various physical constraints. To address this challenge, we present a neural-network-based method for reconstructing mixed states with a high fidelity (∼97.5%) while costing only 50% of the number of measurements typically required for open discrete-time QW in one dimension. Our method uses a neural density operator that models the system and environment, followed by a generalized natural gradient descent procedure that significantly speeds up the training process. Moreover, we introduce a compact interferometric measurement device, improving the scalability of our photonic QW setup that enables experimental learning of mixed states. Our results demonstrate that highly expressive neural networks can serve as powerful alternatives to traditional state tomography. | 2024 Mar 15 | Science advances | No DOI | [
{
"LastName": "Wang",
"FirstName": "Qin-Qin",
"Affiliation": "CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei 230026, China."
},
{
"LastName": "Dong",
"FirstName": "Shaojun",
"Affiliation": "Institute of Artificial Intelligence, Hefei Comprehensive National Science Center, Hefei 230031, China."
},
{
"LastName": "Li",
"FirstName": "Xiao-Wei",
"Affiliation": "Department of Physics, Southern University of Science and Technology, Shenzhen 518055, China."
},
{
"LastName": "Xu",
"FirstName": "Xiao-Ye",
"Affiliation": "CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei 230026, China."
},
{
"LastName": "Wang",
"FirstName": "Chao",
"Affiliation": "Institute of Artificial Intelligence, Hefei Comprehensive National Science Center, Hefei 230031, China."
},
{
"LastName": "Han",
"FirstName": "Shuai",
"Affiliation": "Yangtze Delta Region Industrial Innovation Center of Quantum and Information Technology, Suzhou 215100, China."
},
{
"LastName": "Yung",
"FirstName": "Man-Hong",
"Affiliation": "Institute for Quantum Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China."
},
{
"LastName": "Han",
"FirstName": "Yong-Jian",
"Affiliation": "CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei 230026, China."
},
{
"LastName": "Li",
"FirstName": "Chuan-Feng",
"Affiliation": "CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei 230026, China."
},
{
"LastName": "Guo",
"FirstName": "Guang-Can",
"Affiliation": "CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei 230026, China."
}
] | No |
35602636 | Tackling Explicit Material from Online Video Conferencing Software for Education Using Deep Attention Neural Architectures. | The spread of the COVID-19 pandemic affected all areas of social life, especially education. Globally, many states have closed schools temporarily or imposed local curfews. According to UNESCO estimations, approximately 1.5 billion students have been affected by the closure of schools and the mandatory implementation of distance learning. Although rigorous policies are in place to ban harmful and dangerous content aimed at children, there are many cases where minors, mainly students, have been exposed relatively or unfairly to inappropriate, especially sexual content, during distance learning. Ensuring minors' emotional and mental health is a priority for any education system. This paper presents a severe attention neural architecture to tackle explicit material from online education video conference applications to deal with similar incidents. This is an advanced technique that, for the first time in the literature, proposes an intelligent mechanism that, although it uses attention mechanisms, does not have a square complexity of memory and time in terms of the size of the input. Specifically, we propose the implementation of a Generative Adversarial Network (GAN) with the help of a local, sparse attention mechanism, which can accurately detect obscene and mainly sexual content in streaming online video conferencing software for education. | 2022 | Computational intelligence and neuroscience | No DOI | [
{
"LastName": "Yang",
"FirstName": "Yongzhao",
"Affiliation": "Zhengzhou Preschool Education College, Zhengzhou, Henan 450000, China."
},
{
"LastName": "Xu",
"FirstName": "Shasha",
"Affiliation": "Zhengzhou Preschool Education College, Zhengzhou, Henan 450000, China."
}
] | Yes |
24558751 | The role of neuromediators and cytokines in the pathogenesis of acute traumatic brain injury. | The aim of the study was to investigate quantification of serum serotonin levels and compare the results with inflammatory markers in patients with acute traumatic brain injury. We investigated cytokine, serotonin, psychovegetative status in 72 patients with traumatic brain injury of mild to moderate severity. Increase of serotonin, depending on the severity correlates with the antiinflammatory cytokine markers. Quantitative content of serum serotonin levels may serve as a method of differential diagnosis of brain concussion and contusion. | 2013 | Zhurnal voprosy neirokhirurgii imeni N. N. Burdenko | No DOI | [
{
"LastName": "Selianina",
"FirstName": "N V",
"Affiliation": ""
},
{
"LastName": "Karakulova",
"FirstName": "Iu V",
"Affiliation": ""
},
{
"LastName": "Eroshina",
"FirstName": "O A",
"Affiliation": ""
}
] | No |
18391527 | Synaptonemal complex protein SYCP3 of the rat: evolutionarily conserved domains and the assembly of higher order structures. | SYCP3 is a major structural protein component of vertebrate synaptonemal complexes as well as an important determinant of male fertility, at least in mammals. The elucidation of SYCP3 polymerization properties would provide important information towards our understanding as to how synaptonemal complexes are assembled and disassembled during meiotic prophase. To this end we have investigated the possible contribution of different SYCP3 domains to the assembly of higher order structures. We observed that the evolutionarily conserved domains of the molecule (i.e. the alpha-helix together with the two flanking motifs CM1 and CM2) are not only necessary but also sufficient for SYCP3 polymerization. The relevance of these results for reproduction biology is underscored by recent studies showing that the deletion of the very end of the alpha-helix and CM2 leads to meiosis disruption and infertility in humans. | 2007 | Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation | No DOI | [
{
"LastName": "Baier",
"FirstName": "A",
"Affiliation": "Department of Cell and Developmental Biology, Biocenter, University of Wurzburg, Wurzburg, Germany."
},
{
"LastName": "Alsheimer",
"FirstName": "M",
"Affiliation": ""
},
{
"LastName": "Volff",
"FirstName": "J-N",
"Affiliation": ""
},
{
"LastName": "Benavente",
"FirstName": "R",
"Affiliation": ""
}
] | No |
28487778 | Cl II Malocclusion Treatment, Using the Modified Twin Block Appliance Coordinated with Fixed Orthodontics in a Postmenarche Patient. | Functional appliances have been used for treatment of Class II patients for a long time. The main objective of therapy with functional appliances is to induce supplementary lengthening of the mandible by stimulating increased growth at the condylar cartilage. The Twin Block appliance is one of the most commonly used functional appliances. The aim of this paper is to present a case report of mandibular deficiency treatment with Twin Block appliance in a female patient whose sexual maturation (one and a half years after menarche) and cervical vertebral maturation stage indicated the end of the growth peak. The treatment started with bonding 0.022 in MBT prescription brackets on the upper arch in order to align upper teeth and create a symmetric overjet. When reaching alignment, a modified Twin Block was given to the patient for 8 months. Final coordination was achieved with fixed appliances in both arches. At the end of the treatment, profile of the patient improved, crowding was relieved, and Cl I relationship with normal overjet and overbite was achieved. | 2017 | Case reports in dentistry | No DOI | [
{
"LastName": "Aminian",
"FirstName": "Amin",
"Affiliation": "Kerman Oral and Dental Disease Research Center, Kerman University of Medical Sciences, Kerman, Iran."
},
{
"LastName": "Sarvareh Azimzadeh",
"FirstName": "Shahriar",
"Affiliation": "Kerman Oral and Dental Disease Research Center, Kerman University of Medical Sciences, Kerman, Iran."
},
{
"LastName": "Rahmanian",
"FirstName": "Elina",
"Affiliation": "Kerman Oral and Dental Disease Research Center, Kerman University of Medical Sciences, Kerman, Iran."
}
] | No |
32280643 | Association between Parvovirus B19 and anemia in HIV-infected patients. | Background: Human parvovirus B19 (B19V) can cause anemia in some patients, including those with compromised immunity system. There are a few studies on molecular epidemiology of B19V and its association with anemia in Iran. Therefore, the aim of this study was to determine the B19V DNA, IgM, IgG, genotyping, and viral load in HIV patients in different groups of pregnant women, general population, injection drug users (IDU), and Elite controllers. Also, the possible association of B19V with anemia was studied. Methods: In this case-control study, B19V DNA, anti-B19V IgM, anti-B19V IgG, viral load, and hemoglobin level were assessed in 113 HIV positive patients and 72 healthy controls. Also, CD4+ T cell counts and HIV load were measured in the patients' group. All statistical analyses were done using STATA 14.2 software (Stata Corporation, College Station, Texas, USA). P value < 0.05 was considered statistically significant. Results: Among HIV patients, 19 (16.8%) cases had B19V DNA, 3 (2.7%) had B19V IgM, and 7 (6.2%) had B19V IgG. In control group, the prevalence of B19V DNA, IgM, and IgG was 6 (8.33%), 7(9.7%), and 19 (26.4%), respectively. In subpopulations based on transmission routes, general population had the highest B19V IgG and DNA positivity prevalence and viral load level. There was no significant association between B19V antibodies and DNA with anemia. Conclusion: The results demonstrated that B19V infection cannot be considered as a high-risk factor for anemia in adult HIV patients. However, further studies are needed to determine the exact role of B19V infection in HIV patients. | 2019 | Medical journal of the Islamic Republic of Iran | No DOI | [
{
"LastName": "Nouri",
"FirstName": "Majid",
"Affiliation": "Golestan Hospital Research Center, Tehran, Iran."
},
{
"LastName": "Kamakifar",
"FirstName": "Parvin",
"Affiliation": "Department of Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran."
},
{
"LastName": "Khodabandehlou",
"FirstName": "Niloofar",
"Affiliation": "Department of Internal Medicine, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran."
},
{
"LastName": "Sadri Nahand",
"FirstName": "Javid",
"Affiliation": "Department of Medical Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran."
},
{
"LastName": "Tavakoli",
"FirstName": "Ahmad",
"Affiliation": "Department of Medical Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran."
},
{
"LastName": "Norooznezhad",
"FirstName": "Fatemeh",
"Affiliation": "Infectious Diseases Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran."
},
{
"LastName": "Sorayyayi",
"FirstName": "Saba",
"Affiliation": "Department of Clinical Biochemistry, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran."
},
{
"LastName": "Babaei",
"FirstName": "Farhad",
"Affiliation": "Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran."
},
{
"LastName": "Mostafaei",
"FirstName": "Shayan",
"Affiliation": "Medical Biology Research Center, Institute of Health and Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran."
},
{
"LastName": "Moghoofei",
"FirstName": "Mohsen",
"Affiliation": "Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran."
}
] | No |
38737069 | New Polyether Macrocycles as Promising Antitumor Agents-Targeted Synthesis and Induction of Mitochondrial Apoptosis. | A series of previously unknown aromatic polyether macrodiolides containing a cis,cis-1,5-diene moiety in the molecule were synthesized in 47-74% yields. Macrocycle compounds were first obtained by intermolecular esterification of aromatic polyether diols with α,ω-alka-nZ,(n+4)Z-dienedioic acids mediated by N-(3-(dimethylamino)propyl)-N'-ethylcarbodiimide hydrochloride (EDC·HCl) and 4-(dimethylamino)pyridine (DMAP). For the synthesized compounds, studies of cytotoxicity on tumor (Jurkat, K562, U937), conditionally normal (HEK293) cell lines, and normal fibroblasts were carried out. CC50 was determined, and the therapeutic selectivity index of cytotoxic action (SI) in comparison with normal fibroblasts was evaluated. With the involvement of modern methods of flow cytometry for the most promising macrocycles, their effect on mitochondria and the cell cycle was investigated. It was found that a new macrocycle exhibits pronounced apoptosis-inducing activity toward Jurkat cells and can retard cell division by blocking at the G1/S checkpoint. Also, it was shown that the synthesized macrodiolides influence mitochondria due to their high ability to penetrate the mitochondrial membrane. | 2024 May 7 | ACS omega | No DOI | [
{
"LastName": "Islamov",
"FirstName": "Ilgiz I",
"Affiliation": "Institute of Petrochemistry and Catalysis, Russian Academy of Sciences, 141 Prospekt Oktyabrya, Ufa 450075, Russian Federation."
},
{
"LastName": "Dzhemileva",
"FirstName": "Lilya U",
"Affiliation": "N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt, 47, Moscow 119991, Russian Federation."
},
{
"LastName": "Gaisin",
"FirstName": "Ilgam V",
"Affiliation": "Institute of Petrochemistry and Catalysis, Russian Academy of Sciences, 141 Prospekt Oktyabrya, Ufa 450075, Russian Federation."
},
{
"LastName": "Dzhemilev",
"FirstName": "Usein M",
"Affiliation": "N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt, 47, Moscow 119991, Russian Federation."
},
{
"LastName": "D Yakonov",
"FirstName": "Vladimir A",
"Affiliation": "N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt, 47, Moscow 119991, Russian Federation."
}
] | No |
35992549 | Clinical Features of Dermatomyositis/Polymyositis with Anti-MDA5 Antibody Positivity. | This paper aims to investigate the clinical and laboratory test characteristics of patients with anti-MDA5 antibody-positive PM/DM by analyzing the clinical characteristics, laboratory test results, and 1-year survival rate of patients with anti-MDA5 antibody-positive PM/DM in polymyositis (PM) and dermatomyositis (DM). To further investigate the impact of positive anti-MDA5 antibodies on the prognosis of PM/DM patients. According to the anti-MDA5 antibody test results, 18 cases with positive anti-MDA5 antibodies were in the positive group and 46 cases with negative anti-MDA5 antibodies were in the negative group. The clinical manifestations, laboratory tests, treatment protocols, and prognostic risk factors were collected for both groups. The chi-square test, Mann-Whitney method, Fisher test, t-test, Kaplan-Meier method, and Log-rank test were used for statistical analysis. Anti-MDA5 antibody positivity was more common in patients with DM/CADM. With no statistically significant differences in age and sex ratio between the two groups, The differences in erythrocyte sedimentation rate (ESR), ferritin (Fer), and creatine kinase (CK) levels in the positive group were statistically significant compared with the negative group. Clinically, the positive group was more prone to arthralgia, skin rash, and interstitial pneumonia. | 2022 | Contrast media & molecular imaging | No DOI | [
{
"LastName": "Chen",
"FirstName": "Qiuhe",
"Affiliation": "Department of Rheumatology and Immunology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China."
},
{
"LastName": "Qian",
"FirstName": "Long",
"Affiliation": "Department of Rheumatology and Immunology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China."
}
] | No |
19932130 | From single neuron-firing to consciousness--towards the true solution of the binding problem. | The human brain consists of millions of neural nerve cells being interconnected and firing in parallel in order to process information. A fundamental question is how this parallel neuron-firing can result in a unified experience. This is the so-called binding problem--a problem that is one of today's key questions about brain function and that has puzzled researchers for decades. This article gives a review about the last 50 years of research in this area. It explains what the binding problem is, what classes of binding problems exist, and what the potential solutions suggested so far look like. | 2010 Jun | Neuroscience and biobehavioral reviews | No DOI | [
{
"LastName": "Velik",
"FirstName": "Rosemarie",
"Affiliation": "Tecnalia-Fatronik, Biorobotics, Paseo Mikeletegi 7-Parque Tecnológico, E-20009 Donostia-San Sebastián, Spain. velik.rosi@gmx.at"
}
] | Yes |
31190357 | LncRNA-ROR alleviates hypoxia-triggered damages by downregulating miR-145 in rat cardiomyocytes H9c2 cells. | Chronic hypoxic heart disease (CHD) is a common clinical type of congenital heart disease. Long noncoding RNA regulator of reprogramming (lncRNA-ROR) exerts an important regulating effect in cardiovascular diseases. In our study, we explored the effect of lncRNA-ROR and the possible mechanisms against hypoxia-caused apoptosis in H9c2 cells. H9c2 cells were exposed to hypoxia (1% O2) to construct the in vitro model of CHD. The level of lncRNA-ROR and microRNA (miRNA/miR)-145 was detected. To upregulate the level of lncRNA-ROR and miR-145, transfection was carried out. Western blot assay was performed to quantified protein expression. The interaction of lncRNA-ROR with miR-145 was verified by RIP and Dual-luciferase reporter assays. The expression of p53 and Bax was largely elevated and Bcl-2 was suppressed by hypoxia induction. We found that lncRNA-ROR was elevated by hypoxia. LncRNA-ROR overexpression was able to relieve the damages of H9c2 cells induced by hypoxia through rescuing viability, suppressing apoptosis, and blocking Cytochrome c release. miR-145 was suppressed by overexpressed lncRNA-ROR and the combination of miR-145 mimic was able to abolish the protective effect of lncRNA-ROR. Moreover, we found that lncRNA-ROR activated Ras/Raf/MEK/ERK and PI3K/AKT transduction cascades by suppressing miR-145. Besides, lncRNA-ROR directly targeted miR-145 and negatively modulated the level of miR-145. Our present study revealed that lncRNA-ROR protected H9c2 cells against hypoxia-caused damages by regulation of miR-145 through activating Ras/Raf/MEK/ERK and PI3K/AKT. | 2019 Dec | Journal of cellular physiology | No DOI | [
{
"LastName": "Wang",
"FirstName": "Pengxi",
"Affiliation": "3rd Department of Cardiology, Changyi People's Hospital, Changyi, China."
},
{
"LastName": "Yuan",
"FirstName": "Yanran",
"Affiliation": "Department of Children's Healthcare and Rehabilitation, Jining No.1 People's Hospital, Jining, China."
}
] | Yes |
34306585 | The Effect of lncRNA SNHG3 Overexpression on Lung Adenocarcinoma by Regulating the Expression of miR-890. | The lncRNA small nucleolar host gene 3 (SNHG3) was discovered to play an important role in the occurrence and development of lung adenocarcinoma (LUAD). However, the underlying molecular mechanism of SNHG3 in LUAD remains unclear. In the present study, SNHG3 expression levels in LUAD tissues and cell lines were analyzed using reverse transcription-quantitative PCR. The effects of SNHG3 on the proliferation, apoptosis, migration, and invasion of LUAD cells were determined using Cell Counting Kit-8, colony formation, flow cytometry, wound healing, and Transwell chamber assays, respectively. The specific underlying mechanism of SNHG3 in LUAD was investigated using bioinformatics analysis and a dual luciferase reporter assay. The results revealed that SNHG3 expression levels were downregulated in LUAD tissues and cell lines. Functionally, SNHG3 overexpression suppressed the proliferation, migration, and invasion of LUAD cells, while promoting apoptosis. Mechanistically, microRNA- (miR-) 890 was identified as a potential target of SNHG3, and its expression was negatively regulated by SNHG3. Notably, SNHG3 was found to promote LUAD progression by targeting miR-890. In conclusion, the findings of the present study revealed that lncRNA SNHG3 promoted the occurrence and progression of LUAD by regulating miR-890 expression. | 2021 | Journal of healthcare engineering | No DOI | [
{
"LastName": "Kang",
"FirstName": "Baojie",
"Affiliation": "Department of Respiratory, Weifang Yidu Central Hospital, Weifang City, Shandong, China."
},
{
"LastName": "Qiu",
"FirstName": "Caihong",
"Affiliation": "Department of Respiratory, Weifang Yidu Central Hospital, Weifang City, Shandong, China."
},
{
"LastName": "Zhang",
"FirstName": "Ying",
"Affiliation": "Department of ICU, Zibo Central Hospital, Zibo City, Shandong, China."
}
] | Yes |
17716070 | Attempted and completed suicide in adolescence. | Suicide is the third leading cause of death in adolescence, and medically serious suicide attempts occur in approximately 3% of adolescents. This review examines a number of risk factors that contribute to suicidal behavior. A prior suicide attempt is one of the best predictors of both a repeat attempt and eventual completed suicide. Depression, disruptive behavior disorders, and substance-use disorders also place adolescents at high risk for suicidal behavior, with comorbidity further increasing risk. Research on families indicates that suicidal behavior is transmitted through families. Groups at high risk for suicidal behavior include gay, lesbian, and bisexual youths, incarcerated adolescents, and homeless/runaway teens. Although abnormalities in the serotonergic system have not been consistently linked to suicidal behavior, genetic and neurobiologic studies suggest that impulsive aggression may be the mechanism through which decreased serotonergic activity is related to suicidal behavior. Findings from prevention and intervention studies are modest and indicate the need for substantially more theory-driven treatment research. | 2006 | Annual review of clinical psychology | No DOI | [
{
"LastName": "Spirito",
"FirstName": "Anthony",
"Affiliation": "Center for Alcohol and Addiction Studies and Department of Psychiatry and Human Behavior, Brown University, Providence, Rhode Island 02912, USA. Anthony_Spirito@brown.edu"
},
{
"LastName": "Esposito-Smythers",
"FirstName": "Christianne",
"Affiliation": ""
}
] | No |
38989452 | Spontaneous biases enhance generalization in the neonate brain. | Inductive generalization is adaptive in novel contexts for both biological and artificial intelligence. Spontaneous generalization in inexperienced animals raises questions on whether predispositions (evolutionarily acquired biases, or priors) enable generalization from sparse data, without reinforcement. We exposed neonate chicks to an artificial social partner of a specific color, and then looked at generalization on the red-yellow or blue-green ranges. Generalization was inconsistent with an unbiased model. Biases included asymmetrical generalization gradients, some preferences for unfamiliar stimuli, different speed of learning, faster learning for colors infrequent in the natural spectrum. Generalization was consistent with a Bayesian model that incorporates predispositions as initial preferences and treats the learning process as an update of predispositions. Newborn chicks are evolutionarily prepared for generalization, via biases independent from experience, reinforcement, or supervision. To solve the problem of induction, biological and artificial intelligence can use biases tuned to infrequent stimuli, such as the red and blue colors. | 2024 Jul 19 | iScience | No DOI | [
{
"LastName": "Wang",
"FirstName": "Shuge",
"Affiliation": "School of Biological and Behavioural Sciences, Queen Mary University of London, London, UK."
},
{
"LastName": "Vasas",
"FirstName": "Vera",
"Affiliation": "School of Biological and Behavioural Sciences, Queen Mary University of London, London, UK."
},
{
"LastName": "Freeland",
"FirstName": "Laura",
"Affiliation": "School of Biological and Behavioural Sciences, Queen Mary University of London, London, UK."
},
{
"LastName": "Osorio",
"FirstName": "Daniel",
"Affiliation": "School of Life Sciences, University of Sussex, Brighton, UK."
},
{
"LastName": "Versace",
"FirstName": "Elisabetta",
"Affiliation": "School of Biological and Behavioural Sciences, Queen Mary University of London, London, UK."
}
] | No |
34931138 | Prevention Effects of Chain Management on Pressure Ulcers of Hospitalized Patients. | The study focused on the preventive effects of the chain management model on pressure ulcers in the operating room. Sqoop big data collection module is used to collect patient information from various hospital information systems in a distributed manner. The data were from the clinical data center of the Zhongshan Hospital Xiamen University General Hospital, and 268 patients were selected as the research subjects. A chain management model is constructed, concerning the preventive measures, the management of each link, the perioperative pressure ulcer management, and the reporting of pressure ulcers. Then, the two groups were compared for the SAS and SDS scores before and after nursing, the pressure ulcer sites, pressure ulcer reporting rate, pressure ulcer staging, and nursing satisfaction. The results show that it is not that more collection modules will lead to better cluster performance and that the execution delay is caused by MapReduce requiring the JAVA virtual machine, and after reaching a certain point, the increase in the number of tasks will slow down the process, and as data size increases, DataNote has an expanded capability to analyze data. After nursing treatment, the SAS and SDS scores of the two groups of patients were significantly lower than before treatment (P < 0.05). The pressure ulcers were mainly distributed in the forehead, mandible, cheeks, front chest, and knees in the two groups, and the difference between the two groups was statistically significant (P < 0.05). The total satisfaction of the observation group was 93.28%, and the total satisfaction of the control group was 92.54%. The patients' satisfaction with the chain management model was higher than that of conventional nursing. | 2021 | Journal of healthcare engineering | No DOI | [
{
"LastName": "Yao",
"FirstName": "Jiao",
"Affiliation": "Zhongshan Hospital Xiamen University, Xiamen 361004, China."
},
{
"LastName": "Zhao",
"FirstName": "Jie",
"Affiliation": "The First Affiliated Hospital of Xiamen University, Xiamen 361001, China."
},
{
"LastName": "Chen",
"FirstName": "Tao",
"Affiliation": "Zhongshan Hospital Xiamen University, Xiamen 361004, China."
},
{
"LastName": "Zeng",
"FirstName": "Xuehui",
"Affiliation": "Zhongshan Hospital Xiamen University, Xiamen 361004, China."
}
] | Yes |
35462472 | Improved Metabolic Pathways of Glycolysis, Glycogen Synthesis, the Urea Cycle, and Cytochrome Peroxidase Oxidative Reabsorption in a Miniature Bioreactor. | Bioreactor-based bioartificial liver support systems have had limited success in a translational setting and at preclinical stages. None of the existing systems monitor the metabolic pathways of glycolysis, glycogen synthesis, the urea cycle, and cytochrome peroxidase oxidative reabsorption. Herein, we designed a bioreactor that mimics the human liver microenvironment in vivo and monitors different hepatic metabolic pathways in order to help establish in vitro culture conditions for improved glycolysis, glycogen synthesis, the urea cycle, cytochrome peroxidase oxidative reabsorption and improved hepatic functions in a miniature bioartificial liver. An abnormality in such pathways negatively influences survivability and hepatic functions, including spontaneous liver regeneration. We investigated the metabolic functions of primary mouse adult hepatocytes cultured in a three-dimensional configuration under direct oxygenation conditions (5%, 10%, 20%, and 40% O2) for 14 days in the bioreactor. We analyzed the expression of the genes of hepatic metabolic pathways, such as glycolysis (glucokinase, phosphofructokinase, and pyruvate kinase), glycogen synthesis (glycogen synthetase, UTP glucose-1-phosphate uridylylisomerase, phosphoglucomutase, and glycogen phosphorylase), the urea cycle (arginase, ornithine carbomoyltransferase, fumarate hydratase), oxidative reabsorption (peroxidase), and cytochrome peroxides (catalase and superoxide dismutase), and compared it with the level in vivo. The metabolic mini-map was used to represent the above-mentioned metabolic genes. Increased urea secretion under normoxia and hyperoxia conditions (20% and 40% O2, respectively) was observed, while albumin secretion was decreased in hyperoxic cultures. Lactate formation was up to 15 mg/L[-g]/h[-h]/10[6] cells, 2 mg/L[-g]/h[-h]/10[6] cells, and 0.2 mg/L[-c]/h[-h]/10[6] cells in 5%, 20%, and 40% O2 conditions, respectively while glucose consumption was enhanced under hypoxic conditions (5% and 10% O2). Cellular membrane integrity was estimated by lactate dehydrogenase assay and was found to be negligible in only 20% and 40% O2 conditions. The expression of the phase II enzyme UDP-glucuronosyltransferase was only upregulated in 20% oxygenation. Taken together, 20% O2 was found to be an optimal condition for the long-term culture (up to 14 days) of hepatocytes that promoted the expression of genes in metabolic pathways such as glycolysis, glycogen synthesis, the urea cycle, and cytochrome peroxidase oxidative reabsorption, and improved hepatic functions in a miniature bioreactor for bioartificial liver construction. | 2022 Apr 25 | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | No DOI | [
{
"LastName": "Giri",
"FirstName": "Shibashish",
"Affiliation": "Center for Biotechnology and Biomedicine, Department of Cell Techniques and Applied Stem Cell Biology, University of Leipzig, Leipzig, Germany, shibashish.giri@bbz.uni-leipzig.de; shibashishgermany@gmail.com."
},
{
"LastName": "Schmidt-Heck",
"FirstName": "Wolfgang",
"Affiliation": "Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection, Hans Knöll-Institute, Jena, Germany."
},
{
"LastName": "Bader",
"FirstName": "Augustinus",
"Affiliation": "Center for Biotechnology and Biomedicine, Department of Cell Techniques and Applied Stem Cell Biology, University of Leipzig, Leipzig, Germany."
}
] | No |
28586150 | Analysis of viral infection and biomarkers in patients with acute exacerbation of chronic obstructive pulmonary disease. | To investigate viral infection in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) in Shanghai, and to analyze the clinical characteristics and biomarkers in viral infection. This study included all consecutive patients who were admitted for a diagnosis of AECOPD during June 2013 to May 2015. Thirty-one stable COPD patients and 31 healthy controls were also recruited. Oropharyngeal samples were assessed, PCR for respiratory viruses were performed. Patients were divided into AECOPD virus-positive (+) group and AECOPD virus-negative (-) group according to viral detection. Luminex was used to detect the concentrations of inflammatory cytokines in the serum. A total of 264 patients were included with a mean age of 75 ± 0.5 years. There were 72 patients (27.3%) identified with viral positive, of whom two patients were detected with double viral infections (FluA + FluB and RSVA + HRV, respectively). The rate of viral detection was associated with season, highest in winter. Comparisons of clinical characteristics showed no significant differences between AECOPD virus+ group and AECOPD virus- group. However, serum concentrations of interferon-inducible protein-10 (IP-10) and interferon-gamma (IFN-γ) in virus+ AECOPD patients were significantly higher than those in the virus- AECOPD, stable COPD and healthy control groups (P < .05). Viral infection was an important pathogen in AECOPD patients; the most common viruses included FluA, HRV and FluB. It was very difficult to diagnose the viral infection according to clinical characteristics. The increased of serum IP-10 and IFN-γ levels might be value to indicate viral infection in AECOPD. | 2018 Mar | The clinical respiratory journal | No DOI | [
{
"LastName": "Yin",
"FirstName": "Tiping",
"Affiliation": "Department of Respiratory Medicine, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China."
},
{
"LastName": "Zhu",
"FirstName": "Zhaoqin",
"Affiliation": "Department of Pathogen Diagnosis and Biosafety, Shanghai Public Health Clinical Center, Shanghai, China."
},
{
"LastName": "Mei",
"FirstName": "Zhoufang",
"Affiliation": "Department of Respiratory Medicine, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China."
},
{
"LastName": "Feng",
"FirstName": "Jingjing",
"Affiliation": "Department of Respiratory Medicine, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China."
},
{
"LastName": "Zhang",
"FirstName": "Wanju",
"Affiliation": "Department of Pathogen Diagnosis and Biosafety, Shanghai Public Health Clinical Center, Shanghai, China."
},
{
"LastName": "He",
"FirstName": "Yanchao",
"Affiliation": "Department of Respiratory Medicine, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China."
},
{
"LastName": "Shi",
"FirstName": "Jindong",
"Affiliation": "Department of Respiratory Medicine, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China."
},
{
"LastName": "Qian",
"FirstName": "Ling",
"Affiliation": "Department of Respiratory Medicine, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China."
},
{
"LastName": "Liu",
"FirstName": "Yi",
"Affiliation": "Department of Pathogen Diagnosis and Biosafety, Shanghai Public Health Clinical Center, Shanghai, China."
},
{
"LastName": "Huang",
"FirstName": "Qihui",
"Affiliation": "Department of Respiratory Medicine, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China."
},
{
"LastName": "Hu",
"FirstName": "Yunwen",
"Affiliation": "Department of Pathogen Diagnosis and Biosafety, Shanghai Public Health Clinical Center, Shanghai, China."
},
{
"LastName": "Jie",
"FirstName": "Zhijun",
"Affiliation": "Department of Respiratory Medicine, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China."
}
] | No |
37221388 | Management of Mucinous Cystic Neoplasms of the Liver. | Mucinous cystic neoplasms of the liver (MCN-L) including biliary cystadenomas (BCA) and biliary cystadenocarcinomas (BCAC) are rare cystic lesions that comprise less than 5% of all liver cysts and affect only a small subset of individuals. We herein review the current evidence regarding the clinical presentation, imaging characteristics, tumor markers, pathological findings, clinical management, and prognosis of MCN-L. A comprehensive review of the literature was performed using MEDLINE/Pubmed and Web of Science databases. In PubMed, the terms "biliary cystadenoma," "biliary cystadenocarcinoma," and "non parasitic hepatic cysts" were queried to identify the most recent data on MCN-L. US imaging, CT, and MRI, as well as consideration of clinicopathological features, are required to appropriately characterize and diagnose hepatic cystic tumors. BCA are premalignant lesions and cannot be reliably differentiated from BCAC based on imaging alone. As such, both types of lesions should be treated with margin-negative surgical resection. Following surgical resection, recurrence is fairly low among patients with BCA and BCAC. Despite having worse long-term outcomes than BCA, the prognosis following surgical resection of BCAC still remains more favorable than other primary malignant liver tumors. MCN-L are rare cystic liver tumors that include BCA and BCAC, which can be difficult to differentiate based on imaging alone. Surgical resection remains the mainstay of management for MCN-L with recurrence being generally uncommon. Future multi-institutional studies are still required to better understand the biology behind BCA and BCAC to improve the care of patients with MCN-L. | 2023 Sep | Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract | No DOI | [
{
"LastName": "Aziz",
"FirstName": "Hassan",
"Affiliation": "Department of Surgery, University of Iowa, Iowa City, IA, USA."
},
{
"LastName": "Hamad",
"FirstName": "Ahmad",
"Affiliation": "Department of Surgery, The Ohio State University Wexner Medical Center and James Cancer Hospital and Solove Research Institute, 395 W. 12Th Avenue, Suite 670, Columbus, OH, USA."
},
{
"LastName": "Afyouni",
"FirstName": "Shadi",
"Affiliation": "Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, USA."
},
{
"LastName": "Kamel",
"FirstName": "Ihab R",
"Affiliation": "Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, USA."
},
{
"LastName": "Pawlik",
"FirstName": "Timothy M",
"Affiliation": "Department of Surgery, The Ohio State University Wexner Medical Center and James Cancer Hospital and Solove Research Institute, 395 W. 12Th Avenue, Suite 670, Columbus, OH, USA. tim.pawlik@osumc.edu."
}
] | No |
35571727 | Effect of Micropower Vacuum Dressing on Promoting Wound Healing in Patients with I-II Diabetic Foot. | Discuss the effectiveness and value of micropower vacuum dressing (MVD) in promoting the healing of I-II grades diabetic foot wounds. Sixty patients diagnosed with diabetic foot ulcers and Wagner grades I-II were selected and randomly divided into the control group and experimental group, with 30 cases in each group. The control group was covered with conventional treatments and petrolatum gauze dressings, and the experimental group was treated with MVD on the basis of conventional reatments. The therapeutic effects of the two groups were observed, including healing rate, ulcer area reduction rate, ulcer healing time, dressing change times, ulcer recurrence rate, adverse events, and so on. The healing rate (100%) of the experimental group was higher than that of the control group (56.7%); the wound reduction rate was higher than that of the control group (P < 0.05); the healing time, the number of dressing changes, and the 1-month recurrence rate were all low in the control group (P < 0.05). The incidence of adverse reactions in the experimental group (6.7%) was lower than that in the control group (46.7%) (P < 0.05). MVD has significant effects in the treatment of I-II grades diabetic foot wounds and has few adverse reactions. It is an effective new method that can promote the growth of granulation tissue and epithelium and promote wound healing. | 2022 | Evidence-based complementary and alternative medicine : eCAM | No DOI | [
{
"LastName": "Chen",
"FirstName": "Cunren",
"Affiliation": "Department of Endocrinology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan, China."
},
{
"LastName": "Wang",
"FirstName": "Xixiong",
"Affiliation": "Department of Breast Surgery, Boao Yiling Life Care Center, Qionghai 571400, Hainan, China."
},
{
"LastName": "Liang",
"FirstName": "Changli",
"Affiliation": "Department of Endocrinology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan, China."
},
{
"LastName": "Liu",
"FirstName": "Haiwei",
"Affiliation": "Department of Endocrinology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan, China."
}
] | Yes |
37675581 | Skin Perfusion After Hemostatic Net: An Anatomic and Radiologic Study in a Cadaver Model. | The hemostatic net is a recent technique initially developed to reduce the occurrence of postoperative hematomas following facelift procedures. Its applications have expanded to include skin redraping, deep plane fixation, and other areas beyond the face. However, no experimental study has investigated its effect on skin blood supply. The aim of this study was to analyze facial skin vascularization after applying a hemostatic net to fresh cadavers. Fourteen hemifaces from fresh adult cadavers were examined. The study model involved a deep plane facelift procedure with the use of a hemostatic net. The first step, involving 4 hemifaces, included dissections and two-/three-dimensional angiographies by digital microangiography and computed tomography scan, respectively. The purpose was to evaluate the influence of the hemostatic net on vascular perfusion. The second step involved a sequential dye perfusion study performed on 10 other hemifaces that underwent facelift procedures with the hemostatic net to determine its impact on skin perfusion. The anatomic and radiologic techniques enabled visualization of skin, and showed the arterial system reaching the subdermal vascular plexus and branching between the vascular territories, without interference from the net. The sequential dye perfusion study showed staining after injection in each facelift flap, with comparable coloration distributions before and after the application of the net. The hemostatic net did not affect the skin blood supply, correlating with no clinical increases in ischemia and necrosis rates in the facelift flap. This study provides additional evidence supporting the safety of the hemostatic net in clinical practice. | 2024 Feb 15 | Aesthetic surgery journal | No DOI | [
{
"LastName": "Henry",
"FirstName": "Guillaume",
"Affiliation": ""
},
{
"LastName": "Auersvald",
"FirstName": "Andre",
"Affiliation": ""
},
{
"LastName": "Auersvald",
"FirstName": "Luiz A",
"Affiliation": ""
},
{
"LastName": "Ospital",
"FirstName": "Caroline",
"Affiliation": ""
},
{
"LastName": "Boucher",
"FirstName": "Fabien",
"Affiliation": ""
},
{
"LastName": "Mojallal",
"FirstName": "Ali",
"Affiliation": ""
}
] | No |
38634324 | Transition to suicide ideation and attempt among emergency room patients. | Suicidal thoughts and behaviours (STB) represent a persistent and serious public health problem, and suicide is among the leading causes of death worldwide. We focus on predictors of transition rates and time courses through the STB spectrum among psychiatric emergency room (PER) patients. We aimed to investigate (a) whether currently suicidal patients had prior referrals to the PER, (b) for which reason they were previously referred to the PER and (c) the timing of this referral. We performed a retrospective study spanning 20 years with 24 815 PER referrals. Descriptive statistics of patients' sociodemographic and clinical characteristics are provided and expressed as weighted proportions and means. Logistic regression was used to identify risk profiles of patients who had a higher chance of being referred for reasons of STB given their PER history. Multiple imputation and data weighting techniques were implemented. STB among PER patients was persistent and led to repeated referrals (up to five times more likely), often within a short period (18% <1 month). Those previously referred for ideation/plan had 66% higher risk of making the transition to suicide attempt, with 25% making this transition within a month after previous referral. This is similar to the transition from depressed mood to suicide ideation/plan. STBs in PER patients are persistent and lead to repeated referrals, often within a short period, including transitions to more severe forms of STB. | 2024 Apr 18 | BJPsych open | No DOI | [
{
"LastName": "Yurdadön",
"FirstName": "Claudio",
"Affiliation": "KU Leuven, Leuven, Belgium."
},
{
"LastName": "Bruffaerts",
"FirstName": "Ronny",
"Affiliation": "KU Leuven, Leuven, Belgium."
},
{
"LastName": "Sabbe",
"FirstName": "Marc",
"Affiliation": "KU Leuven, Leuven, Belgium."
},
{
"LastName": "Demyttenaere",
"FirstName": "Koen",
"Affiliation": "KU Leuven, Leuven, Belgium."
},
{
"LastName": "Peeters",
"FirstName": "Elke",
"Affiliation": "Department Medical Affairs, Janssen-Cilag NV, Beerse, Belgium."
},
{
"LastName": "Gericke",
"FirstName": "Franco",
"Affiliation": "KU Leuven, Leuven, Belgium."
},
{
"LastName": "Voorspoels",
"FirstName": "Wouter",
"Affiliation": "KU Leuven, Leuven, Belgium; and UZ Leuven, Leuven, Belgium."
}
] | No |
36690167 | Exercise may alleviate age-related spatial memory impairment by rescuing β-adrenergic receptor dysregulation via both G protein-dependent and β-arrestin-dependent mechanisms in rat hippocampus. | Hippocampal-dependent memory abilities including spatial memory decline with age. Exercise improves memory decline in aging brain, but, the precise mechanisms are still unknown. Learning and memory are recently hypothesized to be mediated by a β-arrestin (βArr)-dependent β-adrenergic pathway. Hence, we examined the effect of 8 weeks of treadmill exercise on hippocampal expression of β-adrenergic receptors (β-ARs; members of the G protein-coupled receptor family), and βArrs as well as spatial learning and memory in aged male rats to determine whether β-AR/βArr pathway could be involved in age-related memory decline. A total of 24 young (3-month-old) and aged (18-month-old) male Wistar rats were divided into young control, aged sedentary, and aged + exercise (n = 8 for each). Western blot for β1- and β2-ARs as well as βArr1 and βArr2 was performed. Spatial learning and memory were evaluated with the Morris water maze. The results showed significant up-regulation of β1-ARs as well as significant down-regulation of β2-AR and βArrs (βArr1 and βArr2) in the hippocampus of aged rats. Spatial memory, but not spatial learning, was impaired in aging, and treadmill exercise improved it. Notably, the improvement in spatial memory was accompanied by amelioration of β-ARs dysregulation and increase in βArr2 levels after exercise. There was a negative association between the expression of βArr2 and β1-AR, but not β2-AR, such that an increase in βArr2 by exercise was associated with reduced β1-AR expression, suggesting βArr2 may contribute to posttranslational down-regulation of β1-ARs. These data suggest that both G protein-dependent and β-arrestin-dependent β-AR pathways may regulate spatial learning and memory in aging brain. | 2023 Apr 1 | Brain research | No DOI | [
{
"LastName": "Chodari",
"FirstName": "Leila",
"Affiliation": "Neurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran; Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran."
},
{
"LastName": "Derafshpour",
"FirstName": "Leila",
"Affiliation": "Neurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran."
},
{
"LastName": "Jafari",
"FirstName": "Abbas",
"Affiliation": "Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran."
},
{
"LastName": "Ghasemi",
"FirstName": "Maedeh",
"Affiliation": "Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran."
},
{
"LastName": "Mehranfard",
"FirstName": "Nasrin",
"Affiliation": "Neurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran. Electronic address: mehranfar.n@umsu.ac.ir."
}
] | No |
35157688 | Vitamin D and diabetes. | Vitamin D insufficiency and diabetes mellitus are two important aspects of endocrine care. Both these conditions are prevalent across the world, and pose a significant challenge to public health. In this article, we explore the various interactions between vitamin D and glucose metabolism. | 2021 Jan | JPMA. The Journal of the Pakistan Medical Association | No DOI | [
{
"LastName": "Kalra",
"FirstName": "Sanjay",
"Affiliation": "Department of Endocrinology, Bharti Hospital, Karnal, India."
},
{
"LastName": "Aggarwal",
"FirstName": "Sameer",
"Affiliation": "Department of Endocrinology, Apex Hospital, Rohtak, India."
}
] | No |
19604131 | Label-free optical biosensor for probing integrative role of adenylyl cyclase in G protein-coupled receptor signaling. | Adenylyl cyclase is considered as an integrator for receptor signaling. However, its integrative role in receptor signaling is largely studied at the level of point of contacts in complex pathways. Here we used forskolin as a pharmacological probe and the resonant waveguide grating (RWG) biosensor to examine the signal integration of G protein-coupled receptors (GPCRs) at the cyclase-cyclic AMP-PKA module. The biosensor is a refractive index sensitive optical biosensor that is capable of detecting ligand-induced dynamic mass redistribution in cells without labels and cellular manipulations. Stimulation of seven cell lines with forskolin led to distinct optical responses, indicative of distinct expressions and/or organization of cyclase isoforms. The forskolin response in A431 was sensitive to the activities of protein kinase A, Rho kinase, and MAP kinases. Desensitization assays showed that the forskolin pretreatment heterologously desensitized G(s) signaling, partially attenuated G(q) signaling, but had complicate impacts on G(i) signaling. This study documents the integrative role of adenylyl cyclase in GPCR signaling and the power of forskolin as a pharmacological probe to differentiate receptor signaling using the label-free biosensor cellular assays. | 2009 | Journal of receptor and signal transduction research | No DOI | [
{
"LastName": "Tran",
"FirstName": "Elizabeth",
"Affiliation": "Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, New York, USA."
},
{
"LastName": "Fang",
"FirstName": "Ye",
"Affiliation": ""
}
] | No |
34659696 | Application of Intelligent Monitoring of Percutaneous Partial Oxygen Pressure in Evaluating the Evolution of Scar Hyperplasia. | The study aimed to investigate the dynamic changes of percutaneous partial oxygen pressure during the development and evolution of a hypertrophic scar. Twenty cases of hypertrophic scar patients at different stages were selected. A percutaneous oxygen monitor was used to measure oxygen partial pressure in the scar and normal skin tissue at 14, 30, 60, and 90 days after surgery. The changes of oxygen partial pressure, tissue structure, HIF-1α, and VEGF expression in the scar tissue were observed, and the correlation was analyzed. In the scar maturation process, with the prolongation of time, the partial oxygen pressure in the tissue increased gradually. The expression intensity of HIF-1α and VEGF decreased gradually, HIF-1α was positively correlated with VEGF (r = 0.98, P < 0.01), there was a negative correlation between oxygen partial pressure and HIF-1 α expression (r = -0.92, P < 0.01), and it was negatively correlated with VEGF (r = -0.88, P < 0.01). TcPO2 measurement can be used to assess scar maturity; HIF-1 α and VEGF may play an essential role in regulating partial oxygen pressure in the scar tissue. | 2021 | Journal of healthcare engineering | No DOI | [
{
"LastName": "Qi",
"FirstName": "Wanle",
"Affiliation": "Department of Burn and Plastic Surgery, Qinghai Provincial People's Hospital, Xining, Qinghai 810000, China."
},
{
"LastName": "Zhuo",
"FirstName": "Mejia",
"Affiliation": "Department of Burn and Plastic Surgery, Qinghai Provincial People's Hospital, Xining, Qinghai 810000, China."
},
{
"LastName": "Tian",
"FirstName": "Yan",
"Affiliation": "Department of Burn and Plastic Surgery, Qinghai Provincial People's Hospital, Xining, Qinghai 810000, China."
},
{
"LastName": "Dawa",
"FirstName": "Zhuoma",
"Affiliation": "Department of Burn and Plastic Surgery, Qinghai Provincial People's Hospital, Xining, Qinghai 810000, China."
},
{
"LastName": "Bao",
"FirstName": "Junjie",
"Affiliation": "Department of Burn and Plastic Surgery, Qinghai Provincial People's Hospital, Xining, Qinghai 810000, China."
},
{
"LastName": "An",
"FirstName": "Yanan",
"Affiliation": "Department of Burn and Plastic Surgery, Qinghai Provincial People's Hospital, Xining, Qinghai 810000, China."
}
] | Yes |
34307679 | Identifying COVID-19-Specific Transcriptomic Biomarkers with Machine Learning Methods. | COVID-19, a severe respiratory disease caused by a new type of coronavirus SARS-CoV-2, has been spreading all over the world. Patients infected with SARS-CoV-2 may have no pathogenic symptoms, i.e., presymptomatic patients and asymptomatic patients. Both patients could further spread the virus to other susceptible people, thereby making the control of COVID-19 difficult. The two major challenges for COVID-19 diagnosis at present are as follows: (1) patients could share similar symptoms with other respiratory infections, and (2) patients may not have any symptoms but could still spread the virus. Therefore, new biomarkers at different omics levels are required for the large-scale screening and diagnosis of COVID-19. Although some initial analyses could identify a group of candidate gene biomarkers for COVID-19, the previous work still could not identify biomarkers capable for clinical use in COVID-19, which requires disease-specific diagnosis compared with other multiple infectious diseases. As an extension of the previous study, optimized machine learning models were applied in the present study to identify some specific qualitative host biomarkers associated with COVID-19 infection on the basis of a publicly released transcriptomic dataset, which included healthy controls and patients with bacterial infection, influenza, COVID-19, and other kinds of coronavirus. This dataset was first analysed by Boruta, Max-Relevance and Min-Redundancy feature selection methods one by one, resulting in a feature list. This list was fed into the incremental feature selection method, incorporating one of the classification algorithms to extract essential biomarkers and build efficient classifiers and classification rules. The capacity of these findings to distinguish COVID-19 with other similar respiratory infectious diseases at the transcriptomic level was also validated, which may improve the efficacy and accuracy of COVID-19 diagnosis. | 2021 | BioMed research international | No DOI | [
{
"LastName": "Chen",
"FirstName": "Lei",
"Affiliation": "School of Life Sciences, Shanghai University, shanghai 200444, China."
},
{
"LastName": "Li",
"FirstName": "Zhandong",
"Affiliation": "College of Food Engineering, Jilin Engineering Normal University, Changchun 130052, China."
},
{
"LastName": "Zeng",
"FirstName": "Tao",
"Affiliation": "Bio-Med Big Data Center, CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, shanghai 200031, China."
},
{
"LastName": "Zhang",
"FirstName": "Yu-Hang",
"Affiliation": "Channing Division of Network Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA."
},
{
"LastName": "Feng",
"FirstName": "KaiYan",
"Affiliation": "Department of Computer Science, Guangdong AIB Polytechnic College, Guangzhou 510507, China."
},
{
"LastName": "Huang",
"FirstName": "Tao",
"Affiliation": "Bio-Med Big Data Center, CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, shanghai 200031, China."
},
{
"LastName": "Cai",
"FirstName": "Yu-Dong",
"Affiliation": "School of Life Sciences, Shanghai University, shanghai 200444, China."
}
] | Yes |
36193074 | TTC7B Is a Novel Prognostic-Related Biomarker in Glioma Correlating with Immune Infiltrates and Response to Oxidative Stress by Temozolomide. | Gliomas are one of the most prevalent malignant brain tumors. Hence, identifying biological markers for glioma is imperative. TTC7B (Tetratricopeptide Repeat Domain 7B) is a gene whose role in cancer in currently identified. To this end, we examined the TTC7B expression as well as its prognostic significance, biological roles, and immune system impacts in patients with glioma. We evaluated the function of TTC7B in GBM and LGG through the published CGGA (Chinese Glioma Genome Atlas) and TCGA (The Cancer Genome Atlas) databases. CIBERSORT and TIMER were used to analyze the link between TTC7B and immune cells, while R was used for statistical analysis. In addition, Transwell analysis, including migration and invasion assays, was performed to identify the relationship between TTC7B and temozolomide. Low expression of TTC7B was observed in GBM and LGG. 1p/19q codeletion, IDH mutation, chemotherapy, and grade were found to have a significant correlation with TTC7B. Besides, low TTC7B expression was linked with low overall survival (OS) in both GBM and LGG. In the Cox analysis, TTC7B was found to independently function as a risk element for OS of patients with glioma. Furthermore, CIBERSORT analysis demonstrated a positive link between TTC7B and multiple immune cells, especially activated NK cells. Transwell analysis, including migration and invasion assays, revealed that temozolomide reduced the migration and invasion capacity of glioma cells and increased the expression of TTC7B. In all, TTC7B could serve as a promising prognostic indicator of LGG and GBM, and is closely associated with immune infiltration and response to oxidative stress by temozolomide. | 2022 | Oxidative medicine and cellular longevity | No DOI | [
{
"LastName": "Chen",
"FirstName": "Zhenhua",
"Affiliation": "Department of Neurosurgery, Affiliated Hospital 2 of Nantong University and First People's Hospital of Nantong City, North Haierxiang Road 6#, Nantong 226001, China."
},
{
"LastName": "Cui",
"FirstName": "Shasha",
"Affiliation": "Nantong Health College of Jiangsu Province, East Zhenxing Road 288#, Nantong 226010, China."
},
{
"LastName": "Dai",
"FirstName": "Yong",
"Affiliation": "Department of Neurosurgery, Affiliated Hospital 2 of Nantong University and First People's Hospital of Nantong City, North Haierxiang Road 6#, Nantong 226001, China."
},
{
"LastName": "Lu",
"FirstName": "Chunfeng",
"Affiliation": "Department of Neurosurgery, Affiliated Hospital 2 of Nantong University and First People's Hospital of Nantong City, North Haierxiang Road 6#, Nantong 226001, China."
},
{
"LastName": "Zhang",
"FirstName": "Huan",
"Affiliation": "Department of Neurosurgery, Affiliated Hospital 2 of Nantong University and First People's Hospital of Nantong City, North Haierxiang Road 6#, Nantong 226001, China."
},
{
"LastName": "Zhao",
"FirstName": "Wei",
"Affiliation": "Department of Neurosurgery, Affiliated Hospital 2 of Nantong University and First People's Hospital of Nantong City, North Haierxiang Road 6#, Nantong 226001, China."
},
{
"LastName": "Yan",
"FirstName": "Hongyan",
"Affiliation": "Department of Neurosurgery, Affiliated Hospital 2 of Nantong University and First People's Hospital of Nantong City, North Haierxiang Road 6#, Nantong 226001, China."
},
{
"LastName": "Zhang",
"FirstName": "Yi",
"Affiliation": "Department of Neurosurgery, Affiliated Hospital 2 of Nantong University and First People's Hospital of Nantong City, North Haierxiang Road 6#, Nantong 226001, China."
}
] | Yes |
20041017 | Pediatric migraine. | Migraine is the most common cause of acute recurrent headaches in children. The pathophysiological concepts have evolved from a purely vascular etiology to a neuroinflammatory process. Clinical evaluation is the mainstay of diagnosis and should also include family history. Investigations help to rule out secondary causes. The role of new drugs in treatment of migraine is discussed and trials are quoted from literature. Indications for starting prophylaxis should be evaluated based on frequency of attacks and influence on quality of life. For management of acute attacks of migraine both acetaminophen and ibuprofen are recommended for use in children. Many drugs like antiepileptic drugs (AED), calcium channel blockers, and antidepressants have been used for prophylaxis of migraine in children. The data for use of newer drugs for migraine in children is limited, though AEDs are emerging a popular choice. Biofeedback and other nonmedicinal therapies are being used with promising results. | 2009 | International journal of pediatrics | No DOI | [
{
"LastName": "Shah",
"FirstName": "Ubaid Hameed",
"Affiliation": "Apollo Centre for Advanced Pediatrics, Indraprastha Apollo Hospital, New Delhi, India."
},
{
"LastName": "Kalra",
"FirstName": "Veena",
"Affiliation": ""
}
] | No |
38909463 | Primary and oxidative source analyses of consumed VOCs in the atmosphere. | Consumed VOCs are the compounds that have reacted to form ozone and secondary organic aerosol (SOA) in the atmosphere. An approach that can apportion the contributions of primary sources and reactions to the consumed VOCs was developed in this study and applied to hourly VOCs data from June to August 2022 measured in Shijiazhuang, China. The results showed that petrochemical industries (36.9 % and 51.7 %) and oxidation formation (20.6 % and 35.6 %) provided the largest contributions to consumed VOCs and OVOCs during the study period, whereas natural gas (5.0 % and 7.6 %) and the mixed source of liquefied petroleum gas and solvent use (3.1 % and 4.2 %) had the relatively low contributions. Compared to the non-O3 pollution (NOP) period, the contributions of oxidation formation, petrochemical industries, and the mixed source of gas evaporation and vehicle emissions to the consumed VOCs during the O3 pollution (OP) period increased by 2.8, 3.8, and 9.3 times, respectively. The differences in contributions of liquified petroleum gas and solvent use, natural gas, and combustion sources to consumed VOCs between OP and NOP periods were relatively small. Transport of petrochemical industries emissions from the southeast to the study site was the primary consumed pathway for VOCs emitted from petrochemical industries. | 2024 Sep 5 | Journal of hazardous materials | No DOI | [
{
"LastName": "Cui",
"FirstName": "Yaqi",
"Affiliation": "State Environmental Protection Key Laboratory of Urban Ambient Air Particulate Matter Pollution Prevention and Control & Tianjin Key Laboratory of Urban Transport Emission Research, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China; CMA-NKU Cooperative Laboratory for Atmospheric Environment-Health Research, Tianjin 300350, China."
},
{
"LastName": "Liu",
"FirstName": "Baoshuang",
"Affiliation": "State Environmental Protection Key Laboratory of Urban Ambient Air Particulate Matter Pollution Prevention and Control & Tianjin Key Laboratory of Urban Transport Emission Research, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China; CMA-NKU Cooperative Laboratory for Atmospheric Environment-Health Research, Tianjin 300350, China. Electronic address: lbsnankai@foxmail.com."
},
{
"LastName": "Yang",
"FirstName": "Yufeng",
"Affiliation": "State Environmental Protection Key Laboratory of Urban Ambient Air Particulate Matter Pollution Prevention and Control & Tianjin Key Laboratory of Urban Transport Emission Research, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China; CMA-NKU Cooperative Laboratory for Atmospheric Environment-Health Research, Tianjin 300350, China."
},
{
"LastName": "Kang",
"FirstName": "Sicong",
"Affiliation": "Beijing Make Environment Science & Technology Co., Ltd., Beijing 100083, China."
},
{
"LastName": "Wang",
"FirstName": "Fuquan",
"Affiliation": "Beijing Make Environment Science & Technology Co., Ltd., Beijing 100083, China."
},
{
"LastName": "Xu",
"FirstName": "Man",
"Affiliation": "Shijiazhuang Environmental Prediction Center, Shijiazhuang 050022, China."
},
{
"LastName": "Wang",
"FirstName": "Wei",
"Affiliation": "Shijiazhuang Environmental Prediction Center, Shijiazhuang 050022, China."
},
{
"LastName": "Feng",
"FirstName": "Yinchang",
"Affiliation": "State Environmental Protection Key Laboratory of Urban Ambient Air Particulate Matter Pollution Prevention and Control & Tianjin Key Laboratory of Urban Transport Emission Research, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China; CMA-NKU Cooperative Laboratory for Atmospheric Environment-Health Research, Tianjin 300350, China."
},
{
"LastName": "Hopke",
"FirstName": "Philip K",
"Affiliation": "Department of Public Health Sciences, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA; Institute for a Sustainable Environment, Clarkson University, Potsdam, NY 13699, USA."
}
] | No |
16912408 | Mechanical regulation of secondary chondrogenesis. | The development of the skull is characterised by its dependence upon epigenetic influences. One of the most important of these is secondary chondrogenesis, which occurs following ossification within certain membrane bone periostea, as a result of biomechanical articulation. We have studied the genesis, character and function of the secondary chondrocytes of the quadratojugal of the chick between embryonic days 11 and 14. Analysis of gene expression revealed that secondary chondrocytes formed coincident with Sox9 upregulation from a precursor population expressing Cbfa1/Runx2: a reversal of the normal sequence. Such secondary chondrocytes rapidly acquired a phenotype that is a compound of prehypertrophic and hypertrophic chondrocytes, exited from the cell cycle and upregulated Ihh. Pulse and pulse/chase experiments with BrdU confirmed the germinal region as the highly proliferative source of the secondary chondrocytes, which formed by division of chondrocyte-committed precursors. By blocking Hh signalling in explant cultures we show that the enhanced proliferation of the germinal region surrounding the secondary chondrocytes derives from this Ihh source. Additionally, in vitro studies on membrane bone periosteal cells (nongerminal region) demonstrated that these cells can also respond to Ihh, and do so both by enhanced proliferation and precocious osteogenesis. Despite the pro-osteogenic effects of Ihh on periosteal cell differentiation, mechanical articulation of the quadratojugal/quadrate joint in explant culture revealed a negative role for articulation in the regulation of osteocalcin by germinal region descendants. Thus, the mechanical stimulus that is the spur to secondary chondrocyte formation appears able to override the osteogenic influence of Ihh on the periosteum, but does not interfere with the cell cycle-promoting component of Hh signalling. | 2006 | Biorheology | No DOI | [
{
"LastName": "Archer",
"FirstName": "Charles W",
"Affiliation": "Connective Tissue Biology Laboratories, School of Biosciences, Cardiff University, Museum Avenue, Cardiff, Wales, UK."
},
{
"LastName": "Buxton",
"FirstName": "Paul",
"Affiliation": ""
},
{
"LastName": "Hall",
"FirstName": "Brian K",
"Affiliation": ""
},
{
"LastName": "Francis-West",
"FirstName": "Philippa",
"Affiliation": ""
}
] | Yes |
36994183 | UPL5 modulates WHY2 protein distribution in a Kub-site dependent ubiquitination in response to [Ca(2+)](cyt)-induced leaf senescence. | The translocation of proteins between various compartments of cells is the simplest and most direct way of an/retrograde communication. However, the mechanism of protein trafficking is far understood. In this study, we showed that the alteration of WHY2 protein abundance in various compartments of cells was dependent on a HECT-type ubiquitin E3 ligase UPL5 interacting with WHY2 in the cytoplasm, plastid, and nucleus, as well as mitochondrion to selectively ubiquitinate various Kub-sites (Kub 45 and Kub 227) of WHY2. Plastid genome stability can be maintained by the UPL5-WHY2 module, accompany by the alteration of photosystem activity and senescence-associated gene expression. In addition, the specificity of UPL5 ubiquitinating various Kub-sites of WHY2 was responded to cold or CaCl2 stress, in a dose [Ca[2+]]cyt-dependent manner. This demonstrates the integration of the UPL5 ubiquitination with the regulation of WHY2 distribution and retrograde communication between organelle and nuclear events of leaf senescence. | 2023 Mar 17 | iScience | No DOI | [
{
"LastName": "Lan",
"FirstName": "Wei",
"Affiliation": "Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China."
},
{
"LastName": "Ma",
"FirstName": "Weibo",
"Affiliation": "Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China."
},
{
"LastName": "Zheng",
"FirstName": "Shuai",
"Affiliation": "Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China."
},
{
"LastName": "Yang",
"FirstName": "Ping",
"Affiliation": "Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China."
},
{
"LastName": "Qiu",
"FirstName": "Yuhao",
"Affiliation": "Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China."
},
{
"LastName": "Lin",
"FirstName": "Wenfang",
"Affiliation": "Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China."
},
{
"LastName": "Ren",
"FirstName": "Yujun",
"Affiliation": "Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China."
},
{
"LastName": "Miao",
"FirstName": "Ying",
"Affiliation": "Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China."
}
] | Yes |
35192423 | Molecular mechanisms for ABCA1-mediated cholesterol efflux. | The maintenance of cellular cholesterol homeostasis is essential for normal cell function and viability. Excessive cholesterol accumulation is detrimental to cells and serves as the molecular basis of many diseases, such as atherosclerosis, Alzheimer's disease, and diabetes mellitus. The peripheral cells do not have the ability to degrade cholesterol. Cholesterol efflux is therefore the only pathway to eliminate excessive cholesterol from these cells. This process is predominantly mediated by ATP-binding cassette transporter A1 (ABCA1), an integral membrane protein. ABCA1 is known to transfer intracellular free cholesterol and phospholipids to apolipoprotein A-I (apoA-I) for generating nascent high-density lipoprotein (nHDL) particles. nHDL can accept more free cholesterol from peripheral cells. Free cholesterol is then converted to cholesteryl ester by lecithin:cholesterol acyltransferase to form mature HDL. HDL-bound cholesterol enters the liver for biliary secretion and fecal excretion. Although how cholesterol is transported by ABCA1 to apoA-I remains incompletely understood, nine models have been proposed to explain this effect. In this review, we focus on the current view of the mechanisms underlying ABCA1-mediated cholesterol efflux to provide an important framework for future investigation and lipid-lowering therapy. | 2022 Jun | Cell cycle (Georgetown, Tex.) | No DOI | [
{
"LastName": "Chen",
"FirstName": "Lei",
"Affiliation": "Department of Cardiology, The Second Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China."
},
{
"LastName": "Zhao",
"FirstName": "Zhen-Wang",
"Affiliation": "Institute of Cardiovascular Disease, Key Lab for Arteriosclerology of Hunan Province, Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic Disease, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang Medical School, University of South China, Hengyang, Hunan, China."
},
{
"LastName": "Zeng",
"FirstName": "Peng-Hui",
"Affiliation": "Department of Clinical Laboratory, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China."
},
{
"LastName": "Zhou",
"FirstName": "Ying-Jie",
"Affiliation": "Department of Clinical Laboratory, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China."
},
{
"LastName": "Yin",
"FirstName": "Wen-Jun",
"Affiliation": "Department of Clinical Laboratory, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China."
}
] | No |
36167949 | Evaluation of Levetiracetam Dosing Strategies for Seizure Prophylaxis Following Traumatic Brain Injury. | Although levetiracetam has been increasingly used as an alternative to phenytoin for early posttraumatic seizure prophylaxis following traumatic brain injury (TBI), an optimal dosing strategy has not been elucidated. The objective of this study is to determine whether different dosing strategies of levetiracetam are associated with the incidence of early posttraumatic seizures when used as prophylaxis following TBI. This retrospective single-center cohort study included admitted patients ≥ 18 years of age with a diagnosis of TBI and receiving levetiracetam for early posttraumatic seizure prophylaxis between July 1, 2013, and September 1, 2019. The primary outcome of this study was to evaluate three different dosing strategies of levetiracetam (≤ 1000 mg/day, 1500 mg/day, and ≥ 2000 mg/day) and associated rates of early posttraumatic seizures. Secondary outcomes were to summarize absolute total daily maintenance doses of levetiracetam among patients who experienced early posttraumatic seizures compared with those who did not, to determine the impact of three different dosing strategies on hospital length of stay and in-hospital mortality, and to assess patient-specific variables on the occurrence of posttraumatic seizures. Overlap propensity score weighting was used to address the potential for confounding. Of the 1287 patients who received levetiracetam for early posttraumatic seizure prophylaxis during the study time frame, 866 patients met eligibility criteria and were included in the study cohort (289 patients in the ≤ 1000 mg/day group, 137 patients in the 1500 mg/day group, and 440 patients in the ≥ 2000 mg/day group). After weighting, the cumulative incidence of early posttraumatic seizure was 2.9% in the ≤ 1000 mg/day group, 8.8% in the 1500 mg/day group, and 9% in the ≥ 2000 mg/day group. The 1500 mg/day and ≥ 2000 mg/day levetiracetam groups had a 209% and 216% increase in the subdistribution hazard of early posttraumatic seizures compared with the ≤ 1000 mg/day levetiracetam group, respectively, but these differences were not statistically significant. In conclusion, the results of this study demonstrate no statistically significant difference in the cumulative incidence of early posttraumatic seizures within 7 days of TBI between three different levetiracetam dosing strategies. After weighting, the ≤ 1000 mg/day levetiracetam group had the lowest rates of early posttraumatic seizures, death without seizure, and in-hospital mortality. | 2023 Apr | Neurocritical care | No DOI | [
{
"LastName": "Ohman",
"FirstName": "Kelsey",
"Affiliation": "Department of Pharmacy, Duke University Hospital, Durham, NC, USA. Kelsey.ohman@duke.edu."
},
{
"LastName": "Kram",
"FirstName": "Bridgette",
"Affiliation": "Department of Pharmacy, Duke University Hospital, Durham, NC, USA."
},
{
"LastName": "Schultheis",
"FirstName": "Jennifer",
"Affiliation": "Department of Pharmacy, Duke University Hospital, Durham, NC, USA."
},
{
"LastName": "Sigmon",
"FirstName": "Jana",
"Affiliation": "Department of Pharmacy, Harris Health System, Harris County, Houston, TX, USA."
},
{
"LastName": "Kaleem",
"FirstName": "Safa",
"Affiliation": "Department of Neurology, NewYork-Presbyterian Weill Cornell Medical Center, New York, NY, USA."
},
{
"LastName": "Yang",
"FirstName": "Zidanyue",
"Affiliation": "Department of Biostatistics and Bioinformatics, Duke University Hospital, Durham, NC, USA."
},
{
"LastName": "Lee",
"FirstName": "Hui-Jie",
"Affiliation": "Department of Biostatistics and Bioinformatics, Duke University Hospital, Durham, NC, USA."
},
{
"LastName": "Vatsaas",
"FirstName": "Cory",
"Affiliation": "Department of Trauma and Critical Care Surgery, Duke University Hospital, Durham, NC, USA."
},
{
"LastName": "Komisarow",
"FirstName": "Jordan",
"Affiliation": "Department of Neurosurgery, Duke University Hospital, Durham, NC, USA."
}
] | No |
37596959 | The social life of natural experiments in epidemiology and public health. | Over the twentieth century, the concept of the natural experiment has become increasingly prominent across a variety of disciplines, albeit most consequentially in epidemiology and public health. Drawing on an analysis of the scientific and medical literature, we explore the social life of the natural experiment, tracing its changing use, meaning and uptake to better understand the work done by the concept. We demonstrate how the natural experiment became central to the identity of post-war epidemiology as the discipline professionalised, turned its attention to the prevention of chronic disease and took centre stage in the field of public health. We then turn to its growing significance amid the rise of evidence-based medicine, and the new meanings natural experiments came to take on in the context of concerns about policy and evidence. Finally, we turn to the newest iteration of the natural experiment in the COVID-19 era, which saw an explosion of studies drawing on the term, albeit in ways that reveal more about the underlying politics of health than the method itself. Throughout, we illustrate that the concept of the natural experiment has always been fundamentally social and political and tied to disciplinary claims-making about evidence and what should count as such. | 2024 Feb | Sociology of health & illness | No DOI | [
{
"LastName": "Herrick",
"FirstName": "Clare",
"Affiliation": "Department of Geography, King's College London, London, UK."
},
{
"LastName": "Bell",
"FirstName": "Kirsten",
"Affiliation": "Department of Global Health and Social Medicine, King's College London, London, UK."
}
] | No |
34480950 | Feasibility of using monocyte-derived dendritic cells obtained from cryopreserved cells for DC-based vaccines. | The study of the effect of cryopreservation on the functionality of monocyte-derived dendritic cells (MDDCs) and dendritic cells (DCs) is essential for their use in different clinical applications such as DCs-based vaccines. Its full maturation and its optimal functionality are crucial for DCs based immunotherapy. In this study, we compared MDDCs derived from fresh and cryopreserved PBMCs in the aspects of phenotype and its effect on T cells at the level of proliferation and cytokine secretion. We pulsed MDDCs obtained from fresh and cryopreserved PBMCs with two different stimuli, CEF and SEA, and the expression maturation markers and cytokine secretion were analyzed. Our results showed that the cryopreservation had no effects in the phenotype of the MDDCs obtained, cell viability, maturation markers expression and/or cytokines secretion, independently whether MDDCs had been generated from fresh or cryopreserved PBMCs. Thus, this study suggests that the use of cryopreserved cells is a good method to keep the cells before use in immunotherapy, avoiding the variability within same individual due to severe blood draws. Even so, the interpretation and comparison of different results should be done considering the different cryopreservation techniques and assays, and their effects on PBMCs, specifically on MDDC and DC cells. | 2021 Nov | Journal of immunological methods | No DOI | [
{
"LastName": "Usero",
"FirstName": "Lorena",
"Affiliation": "AIDS Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) - Hospital Clinic, University of Barcelona, Barcelona, Spain. Electronic address: usero@clinic.cat."
},
{
"LastName": "Miralles",
"FirstName": "Laia",
"Affiliation": "AIDS Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) - Hospital Clinic, University of Barcelona, Barcelona, Spain. Electronic address: lmiralle@clinic.cat."
},
{
"LastName": "Esteban",
"FirstName": "Ignasi",
"Affiliation": "Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain. Electronic address: iesteban@clinic.cat."
},
{
"LastName": "Pastor-Quiñones",
"FirstName": "Carmen",
"Affiliation": "Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain. Electronic address: capastor@clinic.cat."
},
{
"LastName": "Maleno",
"FirstName": "Maria José",
"Affiliation": "AIDS Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) - Hospital Clinic, University of Barcelona, Barcelona, Spain. Electronic address: mjmaleno@clinic.cat."
},
{
"LastName": "Leal",
"FirstName": "Lorna",
"Affiliation": "Infectious Diseases Service and AIDS Research Group, IDIBAPS - Hospital Clinic, University of Barcelona, Barcelona, Spain. Electronic address: laleal@clinic.cat."
},
{
"LastName": "García",
"FirstName": "Felipe",
"Affiliation": "Infectious Diseases Service and AIDS Research Group, IDIBAPS - Hospital Clinic, University of Barcelona, Barcelona, Spain. Electronic address: fgarcia@clinic.cat."
},
{
"LastName": "Plana",
"FirstName": "Montserrat",
"Affiliation": "AIDS Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) - Hospital Clinic, University of Barcelona, Barcelona, Spain. Electronic address: mplana@clinic.cat."
}
] | No |
35154374 | Bereavement in critical care: A narrative review and practice exploration of current provision of support services and future challenges. | This special article outlines the background to bereavement in critical care and scopes the current provision and evidence for bereavement support following death in critical care. Co-authored by a family member and former critical care patient, we aim to draw out the current challenges and think about how and where support can be implemented along the bereavement pathway. We draw on the literature to examine different trajectories of dying in critical care and explore how these might impact bereavement, highlighting important points and risk factors for complicated grief. We present graphic representation of the critical junctures for bereavement in critical care. Adjustment disorders around grief are explored and the consequences for families, including the existing evidence base. Finally, we propose new areas for research in this field. | 2021 Nov | Journal of the Intensive Care Society | No DOI | [
{
"LastName": "Pattison",
"FirstName": "Natalie A",
"Affiliation": "East and North Herts NHS Trust, Hertfordshire, UK; University of Hertfordshire, Hertfordshire, UK; the Florence Nightingale Foundation, London, UK."
},
{
"LastName": "White",
"FirstName": "Catherine",
"Affiliation": "ICU Steps, London, UK."
},
{
"LastName": "Lone",
"FirstName": "Nazir I",
"Affiliation": "University of Edinburgh School of Molecular Genetic and Population Health Sciences, Edinburgh, UK."
}
] | No |
38069549 | Ubiquitin signalling in Drosophila innate immune responses. | Cells respond to invading pathogens and danger signals from the environment by adapting gene expression to meet the need for protective effector molecules. While this innate immune response is required for the cell and the organism to recover, excess immune activation may lead to loss of homeostasis, thereby promoting chronic inflammation and cancer progression. The molecular basis of innate immune defence is comprised of factors promoting survival and proliferation, such as cytokines, antimicrobial peptides and anti-apoptotic proteins. As the molecular mechanisms regulating innate immune responses are conserved through evolution, the fruit fly Drosophila melanogaster serves as a convenient, affordable and ethical model organism to enhance understanding of immune signalling. Fly immunity against bacterial infection is built up by both cellular and humoral responses, where the latter is regulated by the Imd and Toll pathways activating NF-κB transcription factors Relish, Dorsal and Dif, as well as JNK activation and JAK/STAT signalling. As in mammals, the Drosophila innate immune signalling pathways are characterised by ubiquitination of signalling molecules followed by ubiquitin receptors binding to the ubiquitin chains, as well as by rapid changes in protein levels by ubiquitin-mediated targeted proteasomal and lysosomal degradation. In this review, we summarise the molecular signalling pathways regulating immune responses to pathogen infection in Drosophila, with a focus on ubiquitin-dependent control of innate immunity and inflammatory signalling. | 2024 Oct | The FEBS journal | No DOI | [
{
"LastName": "Aalto",
"FirstName": "Anna L",
"Affiliation": "Faculty of Science and Engineering, Cell Biology, Åbo Akademi University, Turku, Finland."
},
{
"LastName": "Luukkonen",
"FirstName": "Veera",
"Affiliation": "Faculty of Science and Engineering, Cell Biology, Åbo Akademi University, Turku, Finland."
},
{
"LastName": "Meinander",
"FirstName": "Annika",
"Affiliation": "Faculty of Science and Engineering, Cell Biology, Åbo Akademi University, Turku, Finland."
}
] | No |
37851176 | The romantic history of signaling pathway discovery in cell death: an updated review. | Cell death is a fundamental physiological process in all living organisms. Processes such as embryonic development, organ formation, tissue growth, organismal immunity, and drug response are accompanied by cell death. In recent years with the development of electron microscopy as well as biological techniques, especially the discovery of novel death modes such as ferroptosis, cuprotosis, alkaliptosis, oxeiptosis, and disulfidptosis, researchers have been promoted to have a deeper understanding of cell death modes. In this systematic review, we examined the current understanding of modes of cell death, including the recently discovered novel death modes. Our analysis highlights the common and unique pathways of these death modes, as well as their impact on surrounding cells and the organism as a whole. Our aim was to provide a comprehensive overview of the current state of research on cell death, with a focus on identifying gaps in our knowledge and opportunities for future investigation. We also presented a new insight for macroscopic intracellular survival patterns, namely that intracellular molecular homeostasis is central to the balance of different cell death modes, and this viewpoint can be well justified by the signaling crosstalk of different death modes. These concepts can facilitate the future research about cell death in clinical diagnosis, drug development, and therapeutic modalities. | 2024 Sep | Molecular and cellular biochemistry | No DOI | [
{
"LastName": "Wang",
"FirstName": "Lei-Yun",
"Affiliation": "Department of Pharmacy, Traditional Chinese and Western Medicine Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, People's Republic of China."
},
{
"LastName": "Liu",
"FirstName": "Xing-Jian",
"Affiliation": "Oujiang Laboratory, Key Laboratory of Alzheimer's Disease of Zhejiang Province, Institute of Aging, Wenzhou Medical University, Wenzhou, 325000, Zhejiang, People's Republic of China."
},
{
"LastName": "Li",
"FirstName": "Qiu-Qi",
"Affiliation": "Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, People's Republic of China."
},
{
"LastName": "Zhu",
"FirstName": "Ying",
"Affiliation": "Department of Pharmacy, Traditional Chinese and Western Medicine Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, People's Republic of China."
},
{
"LastName": "Ren",
"FirstName": "Hui-Li",
"Affiliation": "Department of Pharmacy, Traditional Chinese and Western Medicine Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, People's Republic of China."
},
{
"LastName": "Song",
"FirstName": "Jia-Nan",
"Affiliation": "Oujiang Laboratory, Key Laboratory of Alzheimer's Disease of Zhejiang Province, Institute of Aging, Wenzhou Medical University, Wenzhou, 325000, Zhejiang, People's Republic of China."
},
{
"LastName": "Zeng",
"FirstName": "Jun",
"Affiliation": "Department of Thoracic Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, People's Republic of China."
},
{
"LastName": "Mei",
"FirstName": "Jie",
"Affiliation": "Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, People's Republic of China."
},
{
"LastName": "Tian",
"FirstName": "Hui-Xiang",
"Affiliation": "Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, People's Republic of China. tianhuixiang99@163.com."
},
{
"LastName": "Rong",
"FirstName": "Ding-Chao",
"Affiliation": "Department of Orthopaedic Surgery, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, Guangdong, People's Republic of China. rongdc95@163.com."
},
{
"LastName": "Zhang",
"FirstName": "Shao-Hui",
"Affiliation": "Department of Pharmacy, Traditional Chinese and Western Medicine Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, Hubei, People's Republic of China. zshtjmu@hotmail.com."
}
] | No |
37303681 | Inhibitory effects of Ganoderma lucidum triterpenoid on the growth and metastasis of hepatocellular carcinoma. | To investigate the inhibitory effects and mechanisms of triterpenoids from Ganoderma lucidum (G. lucidum triterpenoids) on the growth and metastasis of hepatocellular carcinoma (HCC) both in vitro and in vivo. In in-vitro experiments, the inhibitory effects of G. lucidum triterpenoids on human HCC SMMC-7721 cell lines were investigated by observing the proliferation, apoptosis, migration and invasion phenotypes of the cell line and assessing the cell cycles as well as the cell apoptosis and proliferation. In in-vivo experiments, nude mouse SMMC-7721 tumor models were established and divided into control group, treatment group A (low concentration group) and treatment group B (high concentration group) according to the treatment models received. Magnetic resonance imaging (MRI) was performed 3 times on each mouse model to calculate their tumor volumes. The liver and kidney functions of the models were evaluated. Tissues harvested from their solid organs were subjected to HE staining, and the tumor tissues were subjected to HE staining and immunohistochemical staining (E-cad, Ki-67, and Tunel), respectively. i. In in-vitro experiments, G. lucidum triterpenoids could inhibit the growth of human HCC SMMC-7721 cell lines via regulating their proliferation and apoptosis phenotype. ii. In in-vivo experiments, the comparison of tumor volumes of mouse models obtained from the second and third MIR scanning was found to be statistically significant between the control group and treatment group A (P<0.05); and statistically significant differences were also found in the tumor volumes from the second and third MRI scanning between the control group and treatment group B (P<0.05). iii. No significant acute injuries or adverse effects were observed in the liver or kidney of the nude mice. G. lucidum triterpenoids could inhibit the growth of tumor cells via blocking their proliferation, accelerating apoptosis, and inhibiting migration and invasion, without marked toxic effects on normal organs and tissues in the body. | 2023 | American journal of translational research | No DOI | [
{
"LastName": "Ding",
"FirstName": "Zhuyuan",
"Affiliation": "Department of Imaging, Ningbo First Hospital Ningbo 315000, Zhejiang, China."
},
{
"LastName": "Zhou",
"FirstName": "Ziyi",
"Affiliation": "Department of Radiology, Maternal and Child Health Hospital of Hubei Province Wuhan 430070, Hubei, China."
},
{
"LastName": "Cheng",
"FirstName": "Xinge",
"Affiliation": "Department of Imaging, Ningbo First Hospital Ningbo 315000, Zhejiang, China."
},
{
"LastName": "Wang",
"FirstName": "Houli",
"Affiliation": "Department of Imaging, Ningbo First Hospital Ningbo 315000, Zhejiang, China."
},
{
"LastName": "Liu",
"FirstName": "Jiayi",
"Affiliation": "Department of Radiology, The Second Xiangya Hospital, Central South University Changsha 410011, Hu'nan, China."
},
{
"LastName": "Cai",
"FirstName": "Yeyu",
"Affiliation": "Department of Radiology, The Second Xiangya Hospital, Central South University Changsha 410011, Hu'nan, China."
},
{
"LastName": "Liu",
"FirstName": "Huan",
"Affiliation": "Department of Radiology, The Second Xiangya Hospital, Central South University Changsha 410011, Hu'nan, China."
},
{
"LastName": "Lv",
"FirstName": "Min",
"Affiliation": "Department of Radiology, The Second Xiangya Hospital, Central South University Changsha 410011, Hu'nan, China."
},
{
"LastName": "Pan",
"FirstName": "Yuning",
"Affiliation": "Department of Imaging, Ningbo First Hospital Ningbo 315000, Zhejiang, China."
},
{
"LastName": "Xiao",
"FirstName": "Enhua",
"Affiliation": "Department of Radiology, The Second Xiangya Hospital, Central South University Changsha 410011, Hu'nan, China."
}
] | No |
36751379 | Safety of Helium-based Plasma Technology for Coagulation of Soft Tissue: A Retrospective Review. | The subdermal application of energy using a helium-based plasma radiofrequency (RF) device has been shown to improve skin laxity. Helium-based plasma RF technology (Renuvion; Apyx Medical, Clearwater, FL) utilizes RF to ionize helium into an electrically conductive plasma capable of coagulating and contracting soft tissue with high precision and minimal thermal spread. This study provides information on the early use of the new generation of electrosurgical generator (APYX-RS3) containing a feature that allows for quantification of the amount of energy delivered to tissue during treatments. To collate procedure details, treatment settings, and safety data in patients treated with a helium-based plasma device for soft tissue coagulation. A retrospective review was conducted of patients aged ≥ 18 years who underwent treatment with a helium-based plasma RF device (Renuvion) for soft tissue coagulation. Demographic data, procedure details, and adverse events were collected. Chart review identified 47 patients with an average age of 45 years and an average BMI of 25.8 kg/m[2]. The amount of energy (J) delivered per treatment area was greatest for abdomen, buttocks, and thighs, with an average of 13.7 kJ, 13.5 kJ, and 10.6 kJ, respectively. No serious, unexpected, or device-related AEs were reported. The use of the generator that quantifies the energy (joules) being applied during the procedure allows the provider to understand and optimize their energy usage. While further research is needed to establish the safety and efficacy of the device for skin tightening, this study provides important information regarding energy application. | 2022 | Aesthetic surgery journal. Open forum | No DOI | [
{
"LastName": "Shridharani",
"FirstName": "Sachin M",
"Affiliation": "Associate clinical professor Department of Plastic Surgery, Washington University St. Louis School of Medicine, St. Louis, MO, USA."
},
{
"LastName": "Kennedy",
"FirstName": "MacKenzie L",
"Affiliation": "Director of clinical research at a private clinic, New York, NY, USA."
}
] | No |
33511133 | Identification of Key Histone Modifications and Their Regulatory Regions on Gene Expression Level Changes in Chronic Myelogenous Leukemia. | Chronic myelogenous leukemia (CML) is a type of cancer with a series of characteristics that make it particularly suitable for observations on leukemogenesis. Research have exhibited that the occurrence and progression of CML are associated with the dynamic alterations of histone modification (HM) patterns. In this study, we analyze the distribution patterns of 11 HM signals and calculate the signal changes of these HMs in CML cell lines as compared with that in normal cell lines. Meanwhile, the impacts of HM signal changes on expression level changes of CML-related genes are investigated. Based on the alterations of HM signals between CML and normal cell lines, the up- and down-regulated genes are predicted by the random forest algorithm to identify the key HMs and their regulatory regions. Research show that H3K79me2, H3K36me3, and H3K27ac are key HMs to expression level changes of CML-related genes in leukemogenesis. Especially H3K79me2 and H3K36me3 perform their important functions in all 100 bins studied. Our research reveals that H3K79me2 and H3K36me3 may be the core HMs for the clinical treatment of CML. | 2020 | Frontiers in cell and developmental biology | No DOI | [
{
"LastName": "Zhang",
"FirstName": "Lu-Qiang",
"Affiliation": "Laboratory of Theoretical Biophysics, School of Physical Science and Technology, Inner Mongolia University, Hohhot, China."
},
{
"LastName": "Fan",
"FirstName": "Guo-Liang",
"Affiliation": "Laboratory of Theoretical Biophysics, School of Physical Science and Technology, Inner Mongolia University, Hohhot, China."
},
{
"LastName": "Liu",
"FirstName": "Jun-Jie",
"Affiliation": "Laboratory of Theoretical Biophysics, School of Physical Science and Technology, Inner Mongolia University, Hohhot, China."
},
{
"LastName": "Liu",
"FirstName": "Li",
"Affiliation": "Laboratory of Theoretical Biophysics, School of Physical Science and Technology, Inner Mongolia University, Hohhot, China."
},
{
"LastName": "Li",
"FirstName": "Qian-Zhong",
"Affiliation": "Laboratory of Theoretical Biophysics, School of Physical Science and Technology, Inner Mongolia University, Hohhot, China."
},
{
"LastName": "Lin",
"FirstName": "Hao",
"Affiliation": "Key Laboratory for Neuro-Information of Ministry of Education, Center for Informational Biology, School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, China."
}
] | No |
38631255 | Metal tolerance protein CsMTP4 has dual functions in maintaining zinc homeostasis in tea plant. | Plants have evolved a series of zinc (Zn) homeostasis mechanisms to cope with the fluctuating Zn in the environment. How Zn is taken up, translocated and tolerate by tea plant remains unknown. In this study, on the basis of RNA-Sequencing, we isolated a plasma membrane-localized Metal Tolerance Protein (MTP) family member CsMTP4 from Zn-deficient tea plant roots and investigated its role in regulation of Zn homeostasis in tea plant. Heterologous expression of CsMTP4 specifically enhanced the tolerance of transgenic yeast to Zn excess. Moreover, overexpression of CsMTP4 in tea plant hairy roots stimulated Zn uptake under Zn deficiency. In addition, CsMTP4 promoted the growth of transgenic Arabidopsis plants by translocating Zn from roots to shoots under Zn deficiency and conferred the tolerance to Zn excess by enhancing the efflux of Zn from root cells. Transcriptome analysis of the CsMTP4 transgenic Arabidopsis found that the expression of Zn metabolism-related genes were differentially regulated compared with wild-type plants when exposed to Zn deficiency and excess conditions. This study provides a mechanistic understanding of Zn uptake and translocation in plants and a new strategy to improve phytoremediation efficiency. | 2024 Jun 5 | Journal of hazardous materials | No DOI | [
{
"LastName": "Li",
"FirstName": "Qinghui",
"Affiliation": "National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, PR China; Joint International Research Laboratory of Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, PR China."
},
{
"LastName": "Zhang",
"FirstName": "Xuyang",
"Affiliation": "National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, PR China; Joint International Research Laboratory of Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, PR China."
},
{
"LastName": "Zhao",
"FirstName": "Peiling",
"Affiliation": "National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, PR China; Joint International Research Laboratory of Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, PR China."
},
{
"LastName": "Chen",
"FirstName": "Yuqiong",
"Affiliation": "National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, PR China; Joint International Research Laboratory of Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, PR China."
},
{
"LastName": "Ni",
"FirstName": "Dejiang",
"Affiliation": "National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, PR China; Joint International Research Laboratory of Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, PR China."
},
{
"LastName": "Wang",
"FirstName": "Mingle",
"Affiliation": "National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, PR China; Joint International Research Laboratory of Germplasm Innovation and Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: wangmingle@mail.hzau.edu.cn."
}
] | No |
37266321 | Development of Novel Herbal Compound Formulations Targeting Neuroinflammation: Network Pharmacology, Molecular Docking, and Experimental Verification. | Neuroinflammation plays an important role in the onset and progression of neurodegenerative diseases. The multicomponent and multitarget approach may provide a practical strategy to address the complex pathological mechanisms of neuroinflammation. This study aimed to develop synergistic herbal compound formulas to attenuate neuroinflammation using integrated network pharmacology, molecular docking, and experimental bioassays. Eight phytochemicals with anti-neuroinflammatory potential were selected in the present study. A compound-gene target-signaling pathway network was constructed to illustrate the mechanisms of action of each phytochemical and the interactions among them at the molecular level. Molecular docking was performed to verify the binding affinity of each phytochemical and its key gene targets. An experimental study was conducted to identify synergistic interactions among the eight phytochemicals, and the associated molecular mechanisms were examined by immunoblotting based on the findings from the network pharmacology analysis. Two paired combinations, andrographolide and 6-shogaol (AN-SG) (IC50 = 2.85 μg/mL), and baicalein-6-shogaol (BA-SG) (IC50 = 3.28 μg/mL), were found to synergistically (combination index <1) inhibit the lipopolysaccharides (LPS)-induced nitric oxide production in microglia N11 cells. Network pharmacology analysis suggested that MAPK14, MAPK8, and NOS3 were the top three relevant gene targets for the three phytochemicals, and molecular docking demonstrated strong binding affinities of the phytochemicals to their coded proteins. Immunoblotting suggested that the AN-SG and BA-SG both showed prominent effects in inhibiting inducible nitric oxide synthase (iNOS) (p < 0.01 and p < 0.05, respectively) and MAPKp-p38 (both p < 0.05) compared with those induced by the LPS stimulation only. The AN-SG combination exhibited greater inhibitions of the protein expressions of iNOS (p < 0.05 vs. individual components), which may partly explain the mechanisms of the synergy observed. This study established a practical approach to developing novel herbal-compound formulations using integrated network pharmacology analysis, molecular docking, and experimental bioassays. The study provides a scientific basis and new insight into the two synergistic combinations against neuroinflammation. | 2023 | Evidence-based complementary and alternative medicine : eCAM | No DOI | [
{
"LastName": "Liu",
"FirstName": "Yang",
"Affiliation": "NICM Health Research Institute, Western Sydney University, Westmead, NSW 2145, Australia."
},
{
"LastName": "Chang",
"FirstName": "Dennis",
"Affiliation": "NICM Health Research Institute, Western Sydney University, Westmead, NSW 2145, Australia."
},
{
"LastName": "Zhou",
"FirstName": "Xian",
"Affiliation": "NICM Health Research Institute, Western Sydney University, Westmead, NSW 2145, Australia."
}
] | No |
37937602 | Dietary phytate induces subclinical mechanical allodynia in mice. | Neuropathic pain is a condition with varying origins, including reduced dietary micronutrient intake. Phytate is a polyphosphate found in seeds and grains that can act as an antinutrient due to the ability of sequester essential divalent metals. Here we tested whether moderate dietary phytate intake could alter nociceptive pain. We subjected weaning mice to a chow supplemented with 1% phytate for eight weeks. Body weight gain, glycemic responses, food ingestion, water ingestion, and liver and adipose tissue weights were not altered compared to controls. We observed a decreased mechanical allodynia threshold in the intervention group, although there were no changes in heat- or cold-induced pain. Animals consuming phytate showed reduced spinal cord tumor necrosis factor (TNF), indicating altered inflammatory process. These data provide evidence for a subclinical induction of mechanical allodynia that is independent of phytate consumption in animals with otherwise normal phenotypic pattern. | 2023 | Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas | No DOI | [
{
"LastName": "Matias",
"FirstName": "D O",
"Affiliation": "Laboratório de Biotecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil."
},
{
"LastName": "Sisnande",
"FirstName": "T",
"Affiliation": "Laboratório de Biotecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil."
},
{
"LastName": "Martins",
"FirstName": "A F",
"Affiliation": "Laboratório de Estudos em Farmacologia Experimental, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil."
},
{
"LastName": "Amaral",
"FirstName": "M J do",
"Affiliation": "Programa de Pós-Graduação em Química Biológica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil."
},
{
"LastName": "Santos",
"FirstName": "B L R",
"Affiliation": "Laboratório de Estudos em Farmacologia Experimental, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil."
},
{
"LastName": "Miranda",
"FirstName": "A L P",
"Affiliation": "Laboratório de Estudos em Farmacologia Experimental, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil."
},
{
"LastName": "Lima",
"FirstName": "L M T R",
"Affiliation": "Laboratório de Biotecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil."
}
] | No |
30815186 | Trust and Sharing in an Interprofessional Environment: A Thematic Analysis From Child Development Support Work in the Community. | Health information technology (HIT) could aid collaboration in the complex, interprofessional space of child development. Trust between stakeholders is necessary to support collaboration, but extant research provides little guidance on designing HIT that promotes trust within interprofessional collaborations. We analyzed interview data obtained from a heterogeneous group of stakeholders (n = 46) including parents and various service providers to explore trust relationships in the child development space. Our thematic analysis revealed that stakeholders assess the trustworthiness of others based on perceived competence, benevolence, integrity, relevance to work, and source of the data. Stakeholders also based trust on the type of data shared, electronic system features or functions, perceived risks and benefits of sharing information, and made trust calculations based on multiple factors. Our research identifies multiple aspects of trust that should be considered in designs for collaborative HIT systems. | 2018 | AMIA ... Annual Symposium proceedings. AMIA Symposium | No DOI | [
{
"LastName": "Mikles",
"FirstName": "Sean P",
"Affiliation": "Biomedical Informatics and Medical Education, University of Washington, Seattle, WA."
},
{
"LastName": "Haldar",
"FirstName": "Shefali",
"Affiliation": "Biomedical Informatics and Medical Education, University of Washington, Seattle, WA."
},
{
"LastName": "Lin",
"FirstName": "Shih-Yin",
"Affiliation": "Biomedical Informatics and Medical Education, University of Washington, Seattle, WA."
},
{
"LastName": "Kientz",
"FirstName": "Julie A",
"Affiliation": "Human Centered Design and Engineering, University of Washington, Seattle, WA."
},
{
"LastName": "Turner",
"FirstName": "Anne M",
"Affiliation": "Biomedical Informatics and Medical Education, University of Washington, Seattle, WA."
}
] | No |
37310260 | Structural and Biophysical Analysis of Adeno-Associated Virus Serotype 2 Capsid Assembly Variants. | Adeno-associated virus (AAV) is a nonenveloped single-stranded DNA (ssDNA) icosahedral T=1 virus being developed as a vector for clinical gene delivery systems. Currently, there are approximately 160 AAV clinical trials, with AAV2 being the most widely studied serotype. To further understand the AAV gene delivery system, this study investigates the role of viral protein (VP) symmetry interactions on capsid assembly, genome packaging, stability, and infectivity. A total of 25 (seven 2-fold, nine 3-fold, and nine 5-fold symmetry interface) AAV2 VP variants were studied. Six 2-fold and two 5-fold variants did not assemble capsids based on native immunoblots and anti-AAV2 enzyme-linked immunosorbent assays (ELISAs). Seven of the 3-fold and seven of the 5-fold variants that assembled capsids were less stable, while the only 2-fold variant that assembled had ~2°C higher thermal stability (Tm) than recombinant wild-type AAV2 (wtAAV2). Three of the 3-fold variants (AAV2-R432A, AAV2-L510A, and N511R) had an approximately 3-log defect in genome packaging. Consistent with previous reports of the 5-fold axes, the region of the capsid is important for VP1u externalization and genome ejection, and one 5-fold variant (R404A) had a significant defect in viral infectivity. The structures of wtAAV2 packaged with a transgene (AAV2-full) and without a transgene (AAV2-empty) and one 5-fold variant (AAV2-R404A) were determined by cryo-electron microscopy and three dimensional (3D)-image reconstruction to 2.8, 2.9, and 3.6 Å resolution, respectively. These structures revealed the role of stabilizing interactions on the assembly, stability, packaging, and infectivity of the virus capsid. This study provides insight into the structural characterization and functional implications of the rational design of AAV vectors. IMPORTANCE Adeno-associated viruses (AAVs) have been shown to be useful vectors for gene therapy applications. Consequently, AAV has been approved as a biologic for the treatment of several monogenic disorders, and many additional clinical trials are ongoing. These successes have generated significant interest in all aspects of the basic biology of AAV. However, to date, there are limited data available on the importance of the capsid viral protein (VP) symmetry-related interactions required to assemble and maintain the stability of the AAV capsids and the infectivity of the AAV capsids. Characterizing the residue type and interactions at these symmetry-driven assembly interfaces of AAV2 has provided the foundation for understanding their role in AAV vectors (serotypes and engineered chimeras) and has determined the residues or regions of the capsid that can or cannot tolerate alterations. | 2023 Jul 27 | Journal of virology | No DOI | [
{
"LastName": "Bennett",
"FirstName": "Antonette",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "Gargas",
"FirstName": "Joseph",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "Kansol",
"FirstName": "Austin",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "Lewis",
"FirstName": "Jordyn",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "Hsi",
"FirstName": "Jane",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "Hull",
"FirstName": "Joshua",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "Mietzsch",
"FirstName": "Mario",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "Tartaglia",
"FirstName": "Lawrence",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "Muzyczka",
"FirstName": "Nicholas",
"Affiliation": "Department of Molecular Genetics and Microbiology and Powell Gene Therapy Center, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "Bhattacharya",
"FirstName": "Nilakshee",
"Affiliation": "Biological Science Imaging Resource, Department of Biological Sciences, Florida State University, Tallahassee, Florida, USA."
},
{
"LastName": "Chipman",
"FirstName": "Paul",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "Agbandje-McKenna",
"FirstName": "Mavis",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
},
{
"LastName": "McKenna",
"FirstName": "Robert",
"Affiliation": "Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA."
}
] | No |
22076676 | Metabolic remodeling precedes mitochondrial outer membrane permeabilization in human glioma xenograft cells. | Glioma cancer cells adapt to changing microenvironment and shift from mitochondrial oxidative phosphorylation to aerobic glycolysis for their metabolic needs irrespective of oxygen availability. In the present study, we show that silencing MMP-9 in combination with uPAR/cathepsin B switch the glycolytic metabolism of glioma cells to oxidative phosphorylation (OXPHOS) and generate reactive oxygen species (ROS) to predispose glioma cells to mitochondrial outer membrane permeabilization. shRNA for MMP-9 and uPAR (pMU) as well as shRNA for MMP-9 and cathepsin B (pMC) activated complexes of mitochondria involved in OXPHOS and inhibited glycolytic hexokinase expression. The decreased interaction of hexokinase 2 with mitochondria in the treated cells indicated the inhibition of glycolysis activation. Overexpression of Akt reversed the pMU- and pMC-mediated OXPHOS to glycolysis switch. The OXPHOS un-coupler oligomycin A altered the expression levels of the Bcl-2 family of proteins; treatment with pMU or pMC reversed this effect and induced mitochondrial outer membrane permeabilization. In addition, our results show changes in mitochondrial pore transition to release cytochrome c due to changes in the VDAC-Bcl-XL and BAX-BAK interaction with pMU and pMC treatments. Taken together, our results suggest that pMU and pMC treatments switch glioma cells from the glycolytic to the OXPHOS pathway through an inhibitory effect on Akt, ROS induction and an increase of cytosolic cytochrome c accumulation. These results demonstrate the potential of pMU and pMC as therapeutic candidates for the treatment of glioma. | 2012 Feb | International journal of oncology | No DOI | [
{
"LastName": "Ponnala",
"FirstName": "Shivani",
"Affiliation": "Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL 61605, USA."
},
{
"LastName": "Chetty",
"FirstName": "Chandramu",
"Affiliation": ""
},
{
"LastName": "Veeravalli",
"FirstName": "Krishna Kumar",
"Affiliation": ""
},
{
"LastName": "Dinh",
"FirstName": "Dzung H",
"Affiliation": ""
},
{
"LastName": "Klopfenstein",
"FirstName": "Jeffrey D",
"Affiliation": ""
},
{
"LastName": "Rao",
"FirstName": "Jasti S",
"Affiliation": ""
}
] | Yes |
35399838 | Can Information Intervention Enhance Consumers' Purchase Intentions of Organic Agricultural Products? A Choice Experiment Based on Organic Milk. | Despite the current rapid growth of organic agriculture, the problem of low demand for organic agricultural products persists in China, and the consumption space warrants improvement. Exploring consumers' preferences for organic agricultural products and increasing their purchase intentions are of utmost significance to promote organic agricultural production. Thus, this study takes organic milk, which accounts for 58% of China's organic processed agricultural products in sales, as the research object, and uses a choice experiment to investigate the influence of consumers on the purchase intention of organic milk under the intervention of environmental protection information and quality and safety information. The main research results revealed that both environmental protection information and quality and safety information have significantly increased consumers' willingness to purchase and that quality and safety information has increased more than environmental protection information. | 2022 | Journal of healthcare engineering | No DOI | [
{
"LastName": "Dou",
"FirstName": "Chang",
"Affiliation": "School of Economics and Management, Northeast Agricultural University, Harbin, Heilongjiang 150030, China."
},
{
"LastName": "Cui",
"FirstName": "Lihang",
"Affiliation": "School of Economics and Management, Northeast Agricultural University, Harbin, Heilongjiang 150030, China."
},
{
"LastName": "Li",
"FirstName": "Cuixia",
"Affiliation": "School of Economics and Management, Northeast Agricultural University, Harbin, Heilongjiang 150030, China."
}
] | Yes |
23724090 | In vivo antioxidant and antiulcer activity of Parkia speciosa ethanolic leaf extract against ethanol-induced gastric ulcer in rats. | The current study was carried out to examine the gastroprotective effects of Parkia speciosa against ethanol-induced gastric mucosa injury in rats. Sprague Dawley rats were separated into 7 groups. Groups 1-2 were orally challenged with carboxymethylcellulose (CMC); group 3 received 20 mg/kg omeprazole and groups 4-7 received 50, 100, 200 and 400 mg/kg of ethanolic leaf extract, respectively. After 1 h, CMC or absolute ethanol was given orally to groups 2-7. The rats were sacrificed after 1 h. Then, the injuries to the gastric mucosa were estimated through assessment of the gastric wall mucus, the gross appearance of ulcer areas, histology, immunohistochemistry and enzymatic assays. Group 2 exhibited significant mucosal injuries, with reduced gastric wall mucus and severe damage to the gastric mucosa, whereas reductions in mucosal injury were observed for groups 4-7. Groups 3-7 demonstrated a reversal in the decrease in Periodic acid-Schiff (PAS) staining induced by ethanol. No symptoms of toxicity or death were observed during the acute toxicity tests. Treatment with the extract led to the upregulation of heat-shock protein 70 (HSP70) and the downregulation of the pro-apoptotic protein BAX. Significant increases in the levels of the antioxidant defense enzymes glutathione (GSH) and superoxide dismutase (SOD) in the gastric mucosal homogenate were observed, whereas that of a lipid peroxidation marker (MDA) was significantly decreased. Significance was defined as p<0.05 compared to the ulcer control group (Group 2). | 2013 | PloS one | No DOI | [
{
"LastName": "Al Batran",
"FirstName": "Rami",
"Affiliation": "Center of Studies for Periodontology, Faculty of Dentistry, University Technology MARA (UiTM), Shah Alam, Selangor, Malaysia."
},
{
"LastName": "Al-Bayaty",
"FirstName": "Fouad",
"Affiliation": ""
},
{
"LastName": "Jamil Al-Obaidi",
"FirstName": "Mazen M",
"Affiliation": ""
},
{
"LastName": "Abdualkader",
"FirstName": "Abdualrahman Mohammed",
"Affiliation": ""
},
{
"LastName": "Hadi",
"FirstName": "Hamid A",
"Affiliation": ""
},
{
"LastName": "Ali",
"FirstName": "Hapipah Mohd",
"Affiliation": ""
},
{
"LastName": "Abdulla",
"FirstName": "Mahmood Ameen",
"Affiliation": ""
}
] | Yes |
35688164 | Efficacy and safety of intramuscular administration of tixagevimab-cilgavimab for early outpatient treatment of COVID-19 (TACKLE): a phase 3, randomised, double-blind, placebo-controlled trial. | Early intramuscular administration of SARS-CoV-2-neutralising monoclonal antibody combination, tixagevimab-cilgavimab, to non-hospitalised adults with mild to moderate COVID-19 has potential to prevent disease progression. We aimed to evaluate the safety and efficacy of tixagevimab-cilgavimab in preventing progression to severe COVID-19 or death. TACKLE is an ongoing, phase 3, randomised, double-blind, placebo-controlled study conducted at 95 sites in the USA, Latin America, Europe, and Japan. Eligible participants were non-hospitalised adults aged 18 years or older with a laboratory-confirmed SARS-CoV-2 infection (determined by RT-PCR or an antigen test) from any respiratory tract specimen collected 3 days or less before enrolment and who had not received a COVID-19 vaccination. A WHO Clinical Progression Scale score from more than 1 to less than 4 was required for inclusion and participants had to receive the study drug 7 days or less from self-reported onset of mild to moderate COVID-19 symptoms or measured fever. Participants were randomly assigned (1:1) to receive either a single tixagevimab-cilgavimab 600 mg dose (two consecutive 3 mL intramuscular injections, one each of 300 mg tixagevimab and 300 mg cilgavimab) or placebo. Randomisation was stratified (using central blocked randomisation with randomly varying block sizes) by time from symptom onset, and high-risk versus low-risk of progression to severe COVID-19. Participants, investigators, and sponsor staff involved in the treatment or clinical evaluation and monitoring of the participants were masked to treatment-group assignments. The primary endpoints were severe COVID-19 or death from any cause through to day 29, and safety. This study is registered with ClinicalTrials.gov, NCT04723394. Between Jan 28, 2021, and July 22, 2021, 1014 participants were enrolled, of whom 910 were randomly assigned to a treatment group (456 to receive tixagevimab-cilgavimab and 454 to receive placebo). The mean age of participants was 46·1 years (SD 15·2). Severe COVID-19 or death occurred in 18 (4%) of 407 participants in the tixagevimab-cilgavimab group versus 37 (9%) of 415 participants in the placebo group (relative risk reduction 50·5% [95% CI 14·6-71·3]; p=0·0096). The absolute risk reduction was 4·5% (95% CI 1·1-8·0; p<0·0001). Adverse events occurred in 132 (29%) of 452 participants in the tixagevimab-cilgavimab group and 163 (36%) of 451 participants in the placebo group, and were mostly of mild or moderate severity. There were three COVID-19-reported deaths in the tixagevimab-cilgavimab group and six in the placebo group. A single intramuscular tixagevimab-cilgavimab dose provided statistically and clinically significant protection against progression to severe COVID-19 or death versus placebo in unvaccinated individuals and safety was favourable. Treating mild to moderate COVID-19 earlier in the disease course with tixagevimab-cilgavimab might lead to more favourable outcomes. AstraZeneca. | 2022 Oct | The Lancet. Respiratory medicine | No DOI | [
{
"LastName": "Montgomery",
"FirstName": "Hugh",
"Affiliation": "Department of Medicine, University College London, London, UK."
},
{
"LastName": "Hobbs",
"FirstName": "F D Richard",
"Affiliation": "Nuffield Department of Primary Care Health Sciences, University of Oxford, Oxford, UK."
},
{
"LastName": "Padilla",
"FirstName": "Francisco",
"Affiliation": "Centro de Investigación en Cardiología y Metabolismo, Guadalajara, Jalisco, Mexico."
},
{
"LastName": "Arbetter",
"FirstName": "Douglas",
"Affiliation": "Biometrics, Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Boston, MA, USA."
},
{
"LastName": "Templeton",
"FirstName": "Alison",
"Affiliation": "Biometrics, Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Cambridge, UK."
},
{
"LastName": "Seegobin",
"FirstName": "Seth",
"Affiliation": "Biometrics, Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Cambridge, UK."
},
{
"LastName": "Kim",
"FirstName": "Kenneth",
"Affiliation": "ARK Clinical Research, Long Beach, CA, USA."
},
{
"LastName": "Campos",
"FirstName": "Jesus Abraham Simón",
"Affiliation": "Köhler & Milstein Research/Hospital Agustín O'Horán, Mérida, Yucatán, Mexico."
},
{
"LastName": "Arends",
"FirstName": "Rosalinda H",
"Affiliation": "Clinical Pharmacology and Safety Sciences, BioPharmaceuticals Research and Development, AstraZeneca, Cambridge, UK."
},
{
"LastName": "Brodek",
"FirstName": "Bryan H",
"Affiliation": "Development Operations, BioPharmaceuticals Research and Development, AstraZeneca, Gaithersburg, MD, USA."
},
{
"LastName": "Brooks",
"FirstName": "Dennis",
"Affiliation": "Patient Safety, Chief Medical Office, BioPharmaceuticals Research and Development, AstraZeneca, Gaithersburg, MD, USA."
},
{
"LastName": "Garbes",
"FirstName": "Pedro",
"Affiliation": "Clinical Pharmacology and Quantitative Pharmacology, Clinical Development, Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Gaithersburg, MD, USA."
},
{
"LastName": "Jimenez",
"FirstName": "Julieta",
"Affiliation": "Clinical Pharmacology and Quantitative Pharmacology, Clinical Development, Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Gaithersburg, MD, USA."
},
{
"LastName": "Koh",
"FirstName": "Gavin C K W",
"Affiliation": "Clinical Development, Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Cambridge, UK."
},
{
"LastName": "Padilla",
"FirstName": "Kelly W",
"Affiliation": "Clinical Development, Late-stage Development, Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Durham, NC, USA."
},
{
"LastName": "Streicher",
"FirstName": "Katie",
"Affiliation": "Translational Medicine, Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Gaithersburg, MD, USA."
},
{
"LastName": "Viani",
"FirstName": "Rolando M",
"Affiliation": "Clinical Pharmacology and Quantitative Pharmacology, Clinical Development, Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Gaithersburg, MD, USA."
},
{
"LastName": "Alagappan",
"FirstName": "Vijay",
"Affiliation": "Late-stage Development, Respiratory and Immunology, BioPharmaceuticals Research and Development, AstraZeneca, Gaithersburg, MD, USA."
},
{
"LastName": "Pangalos",
"FirstName": "Menelas N",
"Affiliation": "Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Cambridge, UK."
},
{
"LastName": "Esser",
"FirstName": "Mark T",
"Affiliation": "Vaccines and Immune Therapies, BioPharmaceuticals Research and Development, AstraZeneca, Gaithersburg, MD, USA. Electronic address: mark.esser@astrazeneca.com."
}
] | No |
31132346 | Comparison of unintended pregnancy at 12 months between two contraceptive care programs; a controlled time-trend design. | To compare unintended pregnancy rates at 12 months between women receiving structured contraceptive counseling plus usual contraceptive care and women receiving structured contraceptive counseling, healthcare provider education and cost support for long-acting reversible contraceptive (LARC) methods. Using a controlled time-trend study design, we first enrolled 502 women receiving structured contraceptive counseling in addition to usual care ("Enhanced Care") and subsequently enrolled 506 women receiving counseling plus healthcare provider education and cost support for LARC methods ("Complete CHOICE") at three federally qualified health centers (FQHCs). Cost support included funds to health centers for "on-the-shelf" LARC methods and no-cost LARC methods for uninsured women. Participants completed in-person baseline surveys and follow-up surveys by telephone at 3, 6 and 12 months. We used Kaplan-Meier survival function to estimate 12-month unintended pregnancy rates and Cox proportional-hazards regression to compare unintended pregnancy rates between the two groups. We imputed pregnancy outcomes for women lost to follow-up (9%) prior to 12 months. "Complete CHOICE" participants were less likely to report an unintended pregnancy at 12 months compared to "Enhanced Care"; 5.3 vs. 9.8 pregnancies per 100 women-years (p=.01). After adjusting for confounders (recruitment site, race, age and federal poverty level), women in "Complete CHOICE" had a 40% lower risk of unintended pregnancy at 12 months (adjusted hazard ratio 0.60; 95% confidence interval 0.37-0.99). Contraceptive provision that includes cost support and healthcare provider education in addition to patient counseling reduced unintended pregnancy at 12 months compared to counseling plus usual contraceptive care. A program of contraceptive care that includes comprehensive counseling; healthcare provider education; cost support; and on-the-shelf, long-acting reversible contraception can reduce unintended pregnancy compared to contraceptive counseling in addition to usual health center care in the FQHC setting. | 2019 Sep | Contraception | No DOI | [
{
"LastName": "Madden",
"FirstName": "Tessa",
"Affiliation": "Division of Family Planning, Department of Obstetrics and Gynecology, Washington University in St. Louis School of Medicine, 4901 Forest Park Avenue, Mailstop: 8064-37-1005, St. Louis, MO 63108, USA. Electronic address: maddent@wustl.edu."
},
{
"LastName": "Paul",
"FirstName": "Rachel",
"Affiliation": "Division of Clinical Research, Department of Obstetrics and Gynecology, Washington University in St. Louis School of Medicine, 4901 Forest Park Avenue, Mailstop: 8064-37-1005, St. Louis, MO 63108, USA."
},
{
"LastName": "Maddipati",
"FirstName": "Ragini",
"Affiliation": "Division of Clinical Research, Department of Obstetrics and Gynecology, Washington University in St. Louis School of Medicine, 4901 Forest Park Avenue, Mailstop: 8064-37-1005, St. Louis, MO 63108, USA."
},
{
"LastName": "Buckel",
"FirstName": "Christina",
"Affiliation": "Division of Clinical Research, Department of Obstetrics and Gynecology, Washington University in St. Louis School of Medicine, 4901 Forest Park Avenue, Mailstop: 8064-37-1005, St. Louis, MO 63108, USA."
},
{
"LastName": "Goodman",
"FirstName": "Melody",
"Affiliation": "College of Global Public Health, New York University, 715 Broadway, 10th Floor, New York, NY 10003, USA."
},
{
"LastName": "Peipert",
"FirstName": "Jeffrey F",
"Affiliation": "Department of Obstetrics and Gynecology, 550 University Blvd, University Hospital 2440, Indiana University School of Medicine, Indianapolis, IN 46202, USA."
}
] | No |
35571489 | Identification of MBOAT2 as an Unfavorable Biomarker Correlated with KRAS Activation and Reduced CD8(+) T-Cell Infiltration in Pancreatic Cancer. | Limited research on the role of membrane-bound O-acyltransferase domain-containing 2 (MBOAT2) in cancer biology exists. In particular, the underlying role of MBOAT2 and its potential mechanisms in pancreatic cancer have not yet been explored. Further study of MBOAT2 could provide new ideas about the carcinogenesis and treatment of pancreatic cancer (PC). In the current study, the potential biological and clinical significances of MBOAT2 were explored by bioinformatics analysis. Real-time quantitative polymerase chain reaction and western blot analysis were performed to determine the level of MBOAT2 in pancreatic ductal adenocarcinoma (PDAC) cell lines. MTT, colony formation, and Transwell assays and flow cytometry of cell cycle were performed to analyze PDAC cell proliferation, migration, and cycle progression. The potential relationship between MBOAT2 level and tumor immunity was analyzed using the ESTIMATE algorithm, CIBERSORT algorithm, and single-sample gene set enrichment analysis. The level of MBOAT2 was remarkably upregulated in most tumors, especially pancreatic tumors, and was positively correlated with a greater rate of tumor recurrence, higher histologic grade, and worse overall survival. MBOAT2 overexpression was also closely correlated with the mutation status and expression level of driver genes, especially KRAS. Meanwhile, functional enrichment analysis demonstrated that MBOAT2 might be involved in cell-cell communication; cell cycling; the Ras signaling pathway; and immune-related biological functions such as the leukocyte activation involved in T-cell-receptor signaling pathway, the inflammatory response, and antigen processing and presentation. Furthermore, in vitro experiments demonstrated that MBOAT2 overexpression accelerated PC cell proliferation and migration. MBOAT2 overexpression also enhanced CDK2 and CCNA2 expression, leading to cell cycle progression from the G1 phase to the G2 phase. Lastly, MBOAT2 overexpression reduced the infiltration level of CD8[+] T-cells, plasmacytoid dendritic cells, and activated dendritic cells but triggered a high type-2 T helper/type-1 T helper cell ration (Th2/Th1 ration) in PC. Our findings suggest that MBOAT2 is a potential protooncogene in PDAC that predicts a poor prognosis and is related to KRAS activation and inferior infiltration of CD8[+] T-cells in PC. | 2022 | Journal of oncology | No DOI | [
{
"LastName": "Li",
"FirstName": "Zhenchong",
"Affiliation": "School of Medicine, South China University of Technology, Guangzhou, 510006 Guangdong Province, China."
},
{
"LastName": "Zhuang",
"FirstName": "Hongkai",
"Affiliation": "Department of Hepatobiliary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510080, China."
},
{
"LastName": "Chen",
"FirstName": "Xinming",
"Affiliation": "Department of Hepatobiliary Surgery, Shenshan Medical Hospital, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Shanwei 516600, China."
},
{
"LastName": "Zhang",
"FirstName": "Yue",
"Affiliation": "Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China."
},
{
"LastName": "Ma",
"FirstName": "Zuyi",
"Affiliation": "Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China."
},
{
"LastName": "Wang",
"FirstName": "Shujie",
"Affiliation": "Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China."
},
{
"LastName": "Yan",
"FirstName": "Qian",
"Affiliation": "School of Medicine, South China University of Technology, Guangzhou, 510006 Guangdong Province, China."
},
{
"LastName": "Zhou",
"FirstName": "Zixuan",
"Affiliation": "School of Medicine, South China University of Technology, Guangzhou, 510006 Guangdong Province, China."
},
{
"LastName": "Huang",
"FirstName": "Shanzhou",
"Affiliation": "School of Medicine, South China University of Technology, Guangzhou, 510006 Guangdong Province, China."
},
{
"LastName": "Zhang",
"FirstName": "Chuanzhao",
"Affiliation": "School of Medicine, South China University of Technology, Guangzhou, 510006 Guangdong Province, China."
},
{
"LastName": "Hou",
"FirstName": "Baohua",
"Affiliation": "School of Medicine, South China University of Technology, Guangzhou, 510006 Guangdong Province, China."
}
] | No |
35913414 | De novo SIX2 activation in human kidneys treated with neonatal kidney stem/progenitor cells. | During development, nephron structures are derived from a SIX2+ stem cell population. After 36 weeks of gestation, these cells are exhausted, and no new nephrons are formed. We have previously described a non-invasive strategy to isolate and expand the native SIX2+ kidney stem cells from the urine of preterm neonates, named neonatal kidney stem/progenitor cells (nKSPC). Here, we investigated the safety and feasibility of administering nKSPC into human kidneys discarded for transplantation during normothermic machine perfusion (NMP) and evaluated the regenerative and immunomodulatory potential of nKSPC treatment. We found that nKSPC administration during NMP is safe and feasible. Interestingly, nKSPC induced the de novo expression of SIX2 in proximal tubular cells of the donor kidneys and upregulated regenerative markers such as SOX9 and VEGF. This is the first time that SIX2 re-expression is observed in adult human kidneys. Moreover, nKSPC administration significantly lowered levels of kidney injury biomarkers and reduced inflammatory cytokine levels via the tryptophan-IDO-kynurenine pathway. In conclusion, nKSPC is a novel cell type to be applied in kidney-targeted cell therapy, with the potential to induce an endogenous regenerative process and immunomodulation. | 2022 Dec | American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons | No DOI | [
{
"LastName": "Arcolino",
"FirstName": "Fanny Oliveira",
"Affiliation": "Department of Development and Regeneration, Cluster Woman and Child, Laboratory of Paediatric Nephrology, KU Leuven, Leuven, Belgium."
},
{
"LastName": "Hosgood",
"FirstName": "Sarah",
"Affiliation": "Department of Surgery, University of Cambridge, Addenbrookes Hospital, Cambridge, UK."
},
{
"LastName": "Akalay",
"FirstName": "Sara",
"Affiliation": "Department of Development and Regeneration, Cluster Woman and Child, Laboratory of Paediatric Nephrology, KU Leuven, Leuven, Belgium."
},
{
"LastName": "Jordan",
"FirstName": "Nina",
"Affiliation": "Department of Surgery, University of Cambridge, Addenbrookes Hospital, Cambridge, UK."
},
{
"LastName": "Herman",
"FirstName": "Jean",
"Affiliation": "Department of Microbiology, Immunology and Transplantation, Laboratory of Molecular Immunology, Rega Institute,KU Leuven, Leuven, Belgium."
},
{
"LastName": "Elliott",
"FirstName": "Tegwen",
"Affiliation": "Department of Surgery, University of Cambridge, Addenbrookes Hospital, Cambridge, UK."
},
{
"LastName": "Veys",
"FirstName": "Koenraad",
"Affiliation": "Department of Development and Regeneration, Cluster Woman and Child, Laboratory of Paediatric Nephrology, KU Leuven, Leuven, Belgium."
},
{
"LastName": "Vermeire",
"FirstName": "Kurt",
"Affiliation": "Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, KU Leuven, Leuven, Belgium."
},
{
"LastName": "Sprangers",
"FirstName": "Ben",
"Affiliation": "Department of Microbiology, Immunology and Transplantation, Laboratory of Molecular Immunology, Rega Institute,KU Leuven, Leuven, Belgium."
},
{
"LastName": "Nicholson",
"FirstName": "Michael",
"Affiliation": "Department of Surgery, University of Cambridge, Addenbrookes Hospital, Cambridge, UK."
},
{
"LastName": "van den Heuvel",
"FirstName": "Lambertus",
"Affiliation": "Department of Development and Regeneration, Cluster Woman and Child, Laboratory of Paediatric Nephrology, KU Leuven, Leuven, Belgium."
},
{
"LastName": "Levtchenko",
"FirstName": "Elena",
"Affiliation": "Department of Development and Regeneration, Cluster Woman and Child, Laboratory of Paediatric Nephrology, KU Leuven, Leuven, Belgium."
}
] | No |
36351074 | Genetic Ancestry Inference from Cancer-Derived Molecular Data across Genomic and Transcriptomic Platforms. | Genetic ancestry-oriented cancer research requires the ability to perform accurate and robust genetic ancestry inference from existing cancer-derived data, including whole-exome sequencing, transcriptome sequencing, and targeted gene panels, very often in the absence of matching cancer-free genomic data. Here we examined the feasibility and accuracy of computational inference of genetic ancestry relying exclusively on cancer-derived data. A data synthesis framework was developed to optimize and assess the performance of the ancestry inference for any given input cancer-derived molecular profile. In its core procedure, the ancestral background of the profiled patient is replaced with one of any number of individuals with known ancestry. The data synthesis framework is applicable to multiple profiling platforms, making it possible to assess the performance of inference specifically for a given molecular profile and separately for each continental-level ancestry; this ability extends to all ancestries, including those without statistically sufficient representation in the existing cancer data. The inference procedure was demonstrated to be accurate and robust in a wide range of sequencing depths. Testing of the approach in four representative cancer types and across three molecular profiling modalities showed that continental-level ancestry of patients can be inferred with high accuracy, as quantified by its agreement with the gold standard of deriving ancestry from matching cancer-free molecular data. This study demonstrates that vast amounts of existing cancer-derived molecular data are potentially amenable to ancestry-oriented studies of the disease without requiring matching cancer-free genomes or patient self-reported ancestry. The development of a computational approach that enables accurate and robust ancestry inference from cancer-derived molecular profiles without matching cancer-free data provides a valuable methodology for genetic ancestry-oriented cancer research. | 2023 Jan 4 | Cancer research | No DOI | [
{
"LastName": "Belleau",
"FirstName": "Pascal",
"Affiliation": "Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York."
},
{
"LastName": "Deschênes",
"FirstName": "Astrid",
"Affiliation": "Cancer Center, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York."
},
{
"LastName": "Chambwe",
"FirstName": "Nyasha",
"Affiliation": "Institute of Molecular Medicine, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, New York."
},
{
"LastName": "Tuveson",
"FirstName": "David A",
"Affiliation": "Cancer Center, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York."
},
{
"LastName": "Krasnitz",
"FirstName": "Alexander",
"Affiliation": "Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York."
}
] | No |
Subsets and Splits